LOCUS       DAXHSJ010000001        58572 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000001 DAXHSJ010000000
VERSION     DAXHSJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 58572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58572
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.001"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(23..715)
                     /locus_tag="ACFM8U_000001"
     CDS             complement(23..715)
                     /locus_tag="ACFM8U_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6333323.1"
                     /translation="MFHAFILRIYTMIKNISKLINHAVLIGFIPNIVFAYPIFAENPA
                     SYQNIHVRSWKARRDSGIVKQDLDFSCGAASIATLLNNFYGRHYSEAEILDKMDKTQM
                     RASFDDMQRIMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGINGETV
                     LLADPSLGHVSMSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNPVRQTR
                     FTVEQIQMRQKR"
     gene            complement(1021..1578)
                     /locus_tag="ACFM8U_000002"
     CDS             complement(1021..1578)
                     /locus_tag="ACFM8U_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333324.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLEIWHGDRVFMH"
     gene            complement(1823..1981)
                     /locus_tag="ACFM8U_000003"
     CDS             complement(1823..1981)
                     /locus_tag="ACFM8U_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333325.1"
                     /translation="MKPPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(1978..2820)
                     /locus_tag="ACFM8U_000004"
     CDS             complement(1978..2820)
                     /locus_tag="ACFM8U_000004"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6333326.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3059..3979
                     /gene="pyrB"
                     /locus_tag="ACFM8U_000005"
     CDS             3059..3979
                     /gene="pyrB"
                     /locus_tag="ACFM8U_000005"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC6333327.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3989..4447
                     /gene="pyrI"
                     /locus_tag="ACFM8U_000006"
     CDS             3989..4447
                     /gene="pyrI"
                     /locus_tag="ACFM8U_000006"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC6333328.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4512..5177
                     /locus_tag="ACFM8U_000007"
     CDS             4512..5177
                     /locus_tag="ACFM8U_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333329.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALDLGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5240..6457)
                     /locus_tag="ACFM8U_000008"
     CDS             complement(5240..6457)
                     /locus_tag="ACFM8U_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6333330.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWSRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6641..7144
                     /gene="def"
                     /locus_tag="ACFM8U_000009"
     CDS             6641..7144
                     /gene="def"
                     /locus_tag="ACFM8U_000009"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC6333331.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLIADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDTVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            7231..8157
                     /gene="fmt"
                     /locus_tag="ACFM8U_000010"
     CDS             7231..8157
                     /gene="fmt"
                     /locus_tag="ACFM8U_000010"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC6333332.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSKS
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8236..9495
                     /gene="rsmB"
                     /locus_tag="ACFM8U_000011"
     CDS             8236..9495
                     /gene="rsmB"
                     /locus_tag="ACFM8U_000011"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC6333333.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9461..10060
                     /locus_tag="ACFM8U_000012"
     CDS             9461..10060
                     /locus_tag="ACFM8U_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC6333334.1"
                     /translation="MVFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLGAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLLLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10060..12177
                     /locus_tag="ACFM8U_000013"
     CDS             10060..12177
                     /locus_tag="ACFM8U_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6333335.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLSGNMGRVLEHY
                     AGSFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPIPKDVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTRL
                     FNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGRLKTFNKAAEQ
                     ILGLSLVPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVQVEYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTETIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMMAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVKTYA"
     gene            12170..13447
                     /locus_tag="ACFM8U_000014"
     CDS             12170..13447
                     /locus_tag="ACFM8U_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC6333336.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKCASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13538..14725
                     /gene="dprA"
                     /locus_tag="ACFM8U_000015"
     CDS             13538..14725
                     /gene="dprA"
                     /locus_tag="ACFM8U_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC6333337.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRASARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRTLSGKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDSPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEA
                     AAGGTAHGGILDKMGFDPVHPDVLAEQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14751..15212
                     /locus_tag="ACFM8U_000016"
     CDS             14751..15212
                     /locus_tag="ACFM8U_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC6333338.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15284..17590
                     /gene="topA"
                     /locus_tag="ACFM8U_000017"
     CDS             15284..17590
                     /gene="topA"
                     /locus_tag="ACFM8U_000017"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC6333339.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNSKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLSNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCTNYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17663..18358)
                     /locus_tag="ACFM8U_000018"
     CDS             complement(17663..18358)
                     /locus_tag="ACFM8U_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333340.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(18378..18929)
                     /locus_tag="ACFM8U_000019"
     CDS             complement(18378..18929)
                     /locus_tag="ACFM8U_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333341.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19048..19428
                     /locus_tag="ACFM8U_000020"
     CDS             19048..19428
                     /locus_tag="ACFM8U_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFC6333342.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVLQINDVDTNSGEFAIYTAQDASVKLDLLKMEL
                     KHCKEMLEHKDKEIELLRKLTETV"
     gene            19517..20086
                     /gene="rsmD"
                     /locus_tag="ACFM8U_000021"
     CDS             19517..20086
                     /gene="rsmD"
                     /locus_tag="ACFM8U_000021"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC6333343.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRSFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20209..20460
                     /locus_tag="ACFM8U_000022"
     CDS             20209..20460
                     /locus_tag="ACFM8U_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC6333344.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            20558..20641
                     /locus_tag="ACFM8U_000023"
     tRNA            20558..20641
                     /locus_tag="ACFM8U_000023"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20592..20594,aa:Tyr,seq:gta)
     gene            20672..20745
                     /locus_tag="ACFM8U_000024"
     tRNA            20672..20745
                     /locus_tag="ACFM8U_000024"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20704..20706,aa:Gly,seq:tcc)
     gene            20755..20829
                     /locus_tag="ACFM8U_000025"
     tRNA            20755..20829
                     /locus_tag="ACFM8U_000025"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20787..20789,aa:Thr,seq:ggt)
     gene            20878..22062
                     /gene="tuf"
                     /locus_tag="ACFM8U_000026"
     CDS             20878..22062
                     /gene="tuf"
                     /locus_tag="ACFM8U_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6333345.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            22069..22144
                     /locus_tag="ACFM8U_000027"
     tRNA            22069..22144
                     /locus_tag="ACFM8U_000027"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22102..22104,aa:Trp,seq:cca)
     gene            22259..22537
                     /gene="secE"
                     /locus_tag="ACFM8U_000028"
     CDS             22259..22537
                     /gene="secE"
                     /locus_tag="ACFM8U_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC6333346.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            22539..23075
                     /gene="nusG"
                     /locus_tag="ACFM8U_000029"
     CDS             22539..23075
                     /gene="nusG"
                     /locus_tag="ACFM8U_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC6333347.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            23176..23610
                     /gene="rplK"
                     /locus_tag="ACFM8U_000030"
     CDS             23176..23610
                     /gene="rplK"
                     /locus_tag="ACFM8U_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC6333348.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            23610..24305
                     /gene="rplA"
                     /locus_tag="ACFM8U_000031"
     CDS             23610..24305
                     /gene="rplA"
                     /locus_tag="ACFM8U_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC6333349.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKTTRVAVFAQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            24535..25035
                     /gene="rplJ"
                     /locus_tag="ACFM8U_000032"
     CDS             24535..25035
                     /gene="rplJ"
                     /locus_tag="ACFM8U_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC6333350.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            25093..25464
                     /gene="rplL"
                     /locus_tag="ACFM8U_000033"
     CDS             25093..25464
                     /gene="rplL"
                     /locus_tag="ACFM8U_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC6333351.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            25655..29833
                     /gene="rpoB"
                     /locus_tag="ACFM8U_000034"
     CDS             25655..29833
                     /gene="rpoB"
                     /locus_tag="ACFM8U_000034"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC6333352.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            29990..34165
                     /gene="rpoC"
                     /locus_tag="ACFM8U_000035"
     CDS             29990..34165
                     /gene="rpoC"
                     /locus_tag="ACFM8U_000035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC6333353.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            34457..34714
                     /locus_tag="ACFM8U_000036"
     CDS             34457..34714
                     /locus_tag="ACFM8U_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333354.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGNRMGMPINIG"
     gene            34711..34860
                     /locus_tag="ACFM8U_000037"
     CDS             34711..34860
                     /locus_tag="ACFM8U_000037"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6333355.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            35039..35410
                     /gene="rpsL"
                     /locus_tag="ACFM8U_000038"
     CDS             35039..35410
                     /gene="rpsL"
                     /locus_tag="ACFM8U_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC6333356.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            35528..35998
                     /gene="rpsG"
                     /locus_tag="ACFM8U_000039"
     CDS             35528..35998
                     /gene="rpsG"
                     /locus_tag="ACFM8U_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC6333357.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            36017..38122
                     /gene="fusA"
                     /locus_tag="ACFM8U_000040"
     CDS             36017..38122
                     /gene="fusA"
                     /locus_tag="ACFM8U_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC6333358.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFIYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            38210..39394
                     /gene="tuf"
                     /locus_tag="ACFM8U_000041"
     CDS             38210..39394
                     /gene="tuf"
                     /locus_tag="ACFM8U_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6333359.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            39412..39723
                     /gene="rpsJ"
                     /locus_tag="ACFM8U_000042"
     CDS             39412..39723
                     /gene="rpsJ"
                     /locus_tag="ACFM8U_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC6333360.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            40014..41066
                     /locus_tag="ACFM8U_000043"
     CDS             40014..41066
                     /locus_tag="ACFM8U_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFC6333361.1"
                     /translation="MFYSQVNKGKMMSLLNVFKDILNNKELNSEERHDLERAVKILQD
                     THVNILITGATGCGKSSTINALFSIDQATGKSDGLVKEVAKVGVGVDPETMDIAKYEL
                     ENMTIWDSPGLGDGEKVDQKHRRNIINKLLEKDSNGNAVIDLVLVLLDGGSRDLGTSY
                     ELINNVIINTLKKSGEGRTDRLLVAINQCDMAMKGRNWDAEAGRPNAALENFLEEKVC
                     STRKRIQEATGVTVNPIYFSAGFKSEDEEQRPYNITRLLRYIITAMPSEKRVIVAEKI
                     NVEEIERDTSRRKQEERQEIDSSIGSALEKILGAAGGVVKDVVSTIASGVGTLIGGAA
                     KAIGGAISTVASFFGW"
     gene            41075..41758
                     /locus_tag="ACFM8U_000044"
     CDS             41075..41758
                     /locus_tag="ACFM8U_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFC6333362.1"
                     /translation="MHRYRISEIGQKVTAAGFRHLDVLLVGATGVGKSSTLNALFGEQ
                     KAKVGTGVDPETQLVESYMLNDVLRFWDSAGLGDGKEADRAHRQKLIDVLSKTYTHSD
                     GQWGWIDLVFVILDGSSRDLGTAYDLLRDVILKMIDPDRVIVAINQADMAMKGRYWDK
                     VLHQPQPNLQQFLDEKAESVQKRILEATGLQISKPVYYSAYENYHLKEIMDAVINHIP
                     VCRRKMHTP"
     gene            41977..42621
                     /gene="rplC"
                     /locus_tag="ACFM8U_000045"
     CDS             41977..42621
                     /gene="rplC"
                     /locus_tag="ACFM8U_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC6333363.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            42621..43241
                     /gene="rplD"
                     /locus_tag="ACFM8U_000046"
     CDS             42621..43241
                     /gene="rplD"
                     /locus_tag="ACFM8U_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC6333364.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            43238..43552
                     /gene="rplW"
                     /locus_tag="ACFM8U_000047"
     CDS             43238..43552
                     /gene="rplW"
                     /locus_tag="ACFM8U_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC6333365.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            43558..44391
                     /gene="rplB"
                     /locus_tag="ACFM8U_000048"
     CDS             43558..44391
                     /gene="rplB"
                     /locus_tag="ACFM8U_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC6333366.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            44398..44676
                     /gene="rpsS"
                     /locus_tag="ACFM8U_000049"
     CDS             44398..44676
                     /gene="rpsS"
                     /locus_tag="ACFM8U_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC6333367.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            44685..45014
                     /gene="rplV"
                     /locus_tag="ACFM8U_000050"
     CDS             44685..45014
                     /gene="rplV"
                     /locus_tag="ACFM8U_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC6333368.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            45024..45716
                     /gene="rpsC"
                     /locus_tag="ACFM8U_000051"
     CDS             45024..45716
                     /gene="rpsC"
                     /locus_tag="ACFM8U_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC6333369.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            45700..46116
                     /gene="rplP"
                     /locus_tag="ACFM8U_000052"
     CDS             45700..46116
                     /gene="rplP"
                     /locus_tag="ACFM8U_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC6333370.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            46116..46307
                     /gene="rpmC"
                     /locus_tag="ACFM8U_000053"
     CDS             46116..46307
                     /gene="rpmC"
                     /locus_tag="ACFM8U_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC6333371.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            46307..46570
                     /gene="rpsQ"
                     /locus_tag="ACFM8U_000054"
     CDS             46307..46570
                     /gene="rpsQ"
                     /locus_tag="ACFM8U_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC6333372.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            46792..47160
                     /gene="rplN"
                     /locus_tag="ACFM8U_000055"
     CDS             46792..47160
                     /gene="rplN"
                     /locus_tag="ACFM8U_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC6333373.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            47172..47495
                     /gene="rplX"
                     /locus_tag="ACFM8U_000056"
     CDS             47172..47495
                     /gene="rplX"
                     /locus_tag="ACFM8U_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC6333374.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            47505..48044
                     /gene="rplE"
                     /locus_tag="ACFM8U_000057"
     CDS             47505..48044
                     /gene="rplE"
                     /locus_tag="ACFM8U_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC6333375.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            48047..48352
                     /gene="rpsN"
                     /locus_tag="ACFM8U_000058"
     CDS             48047..48352
                     /gene="rpsN"
                     /locus_tag="ACFM8U_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC6333376.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            48368..48760
                     /gene="rpsH"
                     /locus_tag="ACFM8U_000059"
     CDS             48368..48760
                     /gene="rpsH"
                     /locus_tag="ACFM8U_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC6333377.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            48774..49307
                     /gene="rplF"
                     /locus_tag="ACFM8U_000060"
     CDS             48774..49307
                     /gene="rplF"
                     /locus_tag="ACFM8U_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC6333378.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            49321..49674
                     /gene="rplR"
                     /locus_tag="ACFM8U_000061"
     CDS             49321..49674
                     /gene="rplR"
                     /locus_tag="ACFM8U_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC6333379.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            49693..50211
                     /gene="rpsE"
                     /locus_tag="ACFM8U_000062"
     CDS             49693..50211
                     /gene="rpsE"
                     /locus_tag="ACFM8U_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC6333380.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            50204..50389
                     /gene="rpmD"
                     /locus_tag="ACFM8U_000063"
     CDS             50204..50389
                     /gene="rpmD"
                     /locus_tag="ACFM8U_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC6333381.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            50391..50825
                     /gene="rplO"
                     /locus_tag="ACFM8U_000064"
     CDS             50391..50825
                     /gene="rplO"
                     /locus_tag="ACFM8U_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC6333382.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            50837..52147
                     /gene="secY"
                     /locus_tag="ACFM8U_000065"
     CDS             50837..52147
                     /gene="secY"
                     /locus_tag="ACFM8U_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC6333383.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            52152..52370
                     /gene="infA"
                     /locus_tag="ACFM8U_000066"
     CDS             52152..52370
                     /gene="infA"
                     /locus_tag="ACFM8U_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC6333384.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            52391..52504
                     /gene="rpmJ"
                     /locus_tag="ACFM8U_000067"
     CDS             52391..52504
                     /gene="rpmJ"
                     /locus_tag="ACFM8U_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC6333385.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            52570..52932
                     /gene="rpsM"
                     /locus_tag="ACFM8U_000068"
     CDS             52570..52932
                     /gene="rpsM"
                     /locus_tag="ACFM8U_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC6333386.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            52952..53347
                     /gene="rpsK"
                     /locus_tag="ACFM8U_000069"
     CDS             52952..53347
                     /gene="rpsK"
                     /locus_tag="ACFM8U_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC6333387.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            53367..53987
                     /gene="rpsD"
                     /locus_tag="ACFM8U_000070"
     CDS             53367..53987
                     /gene="rpsD"
                     /locus_tag="ACFM8U_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC6333388.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            54013..54999
                     /gene="rpoA"
                     /locus_tag="ACFM8U_000071"
     CDS             54013..54999
                     /gene="rpoA"
                     /locus_tag="ACFM8U_000071"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC6333389.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            55023..55391
                     /gene="rplQ"
                     /locus_tag="ACFM8U_000072"
     CDS             55023..55391
                     /gene="rplQ"
                     /locus_tag="ACFM8U_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC6333390.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            55538..56251
                     /gene="minC"
                     /locus_tag="ACFM8U_000073"
     CDS             55538..56251
                     /gene="minC"
                     /locus_tag="ACFM8U_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC6333391.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            56279..57094
                     /gene="minD"
                     /locus_tag="ACFM8U_000074"
     CDS             56279..57094
                     /gene="minD"
                     /locus_tag="ACFM8U_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC6333392.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            57098..57361
                     /gene="minE"
                     /locus_tag="ACFM8U_000075"
     CDS             57098..57361
                     /gene="minE"
                     /locus_tag="ACFM8U_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC6333393.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            57365..58285
                     /gene="oxyR"
                     /locus_tag="ACFM8U_000076"
     CDS             57365..58285
                     /gene="oxyR"
                     /locus_tag="ACFM8U_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC6333394.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPQMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(58282..58473)
                     /locus_tag="ACFM8U_000077"
     CDS             complement(58282..58473)
                     /locus_tag="ACFM8U_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333395.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
BASE COUNT        15451 a        13125 c        15278 g        14718 t
ORIGIN      
        1 taaatgggca aaaatcggtt tattatcgtt tttgccgcat ttggatttgt tctaccgtaa
       61 aacgtgtttg acgaacggga ttcttattaa aaaacatctg atttctaaca aaatcagtat
      121 tttttggcac gatggctaaa atttttcctt ccatttcgcc atcacgtgtt ttccatgcgc
      181 tcaagaattg tgatttgctc attgagacgt gccccagcga tggatcagcc agcaaaacag
      241 tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat tttaaataca
      301 caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa ccttgtgctt
      361 caaaacccag ttcgggcatt atgcgttgca tatcatcaaa agaagcacgc atttgggttt
      421 tatccatttt gtccaagatt tccgcttcag aataatgtct gccataaaaa ttattcagta
      481 acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact atgccggaat
      541 ctcgccgtgc tttccaactc cgtacatgga tgttttggta agaagcgggg ttttcagcaa
      601 atatagggta agcaaaaaca atatttggga taaagccaat taaaaccgca tggtttatta
      661 atttagaaat attttttatc atagtatata tgcgtagaat aaacgcatga aacataaaaa
      721 aataaaatca tatgcaattt tattgcatga ataaatatga ataaaataga taatgatggg
      781 agtaaatacg ccatgtattt tggaagttta aatttattaa taataaaata atttatcgta
      841 gcgcaaataa atcccaaaat tggaacgatt agaaaaaaaa ttaatacacc catcatcatg
      901 caaactctat attaataaat agctaactaa ttctattgtg gaaattagtt agctaactaa
      961 aagttattaa tgattatttc cgagaattga ctgcattgtt ggcagcattg gccccaaaac
     1021 ctagtgcatg aatacccggt ctccatgcca aatttccagc caatccgcct ccggcagcag
     1081 cagccagccc tgttttgccg ctactcctgt tgccgcaccg attcctgccg cagtagccgc
     1141 gccttgcgca gttcctaatt taccatgatt atacaaatta gcaccatgat actcccatgc
     1201 acctaatgca ccgccaaaag cagcggctgc aataatggga acaaattcac cttgtgtttc
     1261 tttcatttca gcctgtgata attgaattgc tttcacattt tggctgtcaa aaacttggct
     1321 gtctaaattt tgcgccatta caggtgtaat catcatagcc attacagttg caattttcgt
     1381 tgcgctggtt tgcacataaa taggattagc aaattcgctt tgattgcgtt cagtgttgat
     1441 gtagctaata ctgctttcta gtttgaattt acccttgtca gtcaataatt cttccaaact
     1501 taaaggtaag tcagcataag caatttggct ggcaacagtc aaaataaaat ctattagaca
     1561 tttgtgtttt tgcatcattt cgtttgattt tctagttttt gagaatgata caaagttttt
     1621 ttacaaagta aagagtcact ctgaaaaaca ttttttcatt ataaatcaaa atattgatag
     1681 aataaatagc gagcatcgat tcacggtgta ctttagtgca aagcgtacaa cgcgagcctg
     1741 aaccaccagc cgcaacagga aaagaaagcc gatagagtgc aatgcttgcc aacgtgcaag
     1801 cgagcttgca agaacgcttg gctcaacgag agcaggcaag acagaaagca gaaagcagga
     1861 taggagcggt aacgcaaagg tctcgggctt tgatttcgcc gtaaaccctg ctgccgcctt
     1921 gtccggaaag ggtgcaggcg gcgagtgccg acagggtgca gatggggagg gggggtttca
     1981 tttggggtcg caacggaagt ggtatgcgca gatttcaaaa ccgtttttga aatacaggcg
     2041 gtgcgcgtcg gcacggtcgt ggttgacgtg gacgttgagg tggattttgg ttacccctgt
     2101 ttccgcgccg attttgcgga cttcttccaa aaggcgcgag gcgtagcctt tgcggcggct
     2161 ttgcggcagg gtaacgatgt catcgatgtg gatgtggcgg ccgctggcga gggtgcaggc
     2221 ttcgcggaag ccgcagacgg cgacggcatt gtgtttgcct tcttcaaaaa tacccagcag
     2281 gcggtagcct tgggggcgtt ggactttgtt gatctgttcg gtaaagcggt tgatgtcggt
     2341 cagggcggaa cgcaaaacgc tcaaggctgc aaaggcggtg gcggtgtcgt ccgcgccgat
     2401 ttcgcgcaaa acgtaggatg cgcccgaggc ggtctgttcc tgtgctttct cggcggcgtg
     2461 tttttcttcg attgcctgtg ccagcatgac gtgttcgtcg gcagggttgt tttgtccgcc
     2521 ctgttcgcgt tcttcgagca gggctttgca gtcgatgacg cgcaggtcgt tgtcggcggc
     2581 aaagtccatc aggaagcgga acatttgggg gttgtcggtt tccagttttt tatcgacggc
     2641 gacgcagcgg atgttgtcca ccagcatggg gcgcacccat ttcgaatagg aaagcctgtg
     2701 gtctttggtg aacgaggaca gaatgccgca caggcgttcc gcccagtcgc tgggacggaa
     2761 aatcttgccg gaactcgttg tgccgtggat gacgacttcg taggggttgc agactaacat
     2821 ggcggcttcc tgaaaagaaa tgtctggcgc gattatacct tatgcttatg cgggcgtgtt
     2881 tggatatgcc gtctgaaaag tacgggattc gtgcggtaaa actttgcggc ggcaaatgtg
     2941 cgataatacg cgccgtattg ccgcttttgc gaagctgttc cgcaaacata cgggcggcgt
     3001 ggacgacgta taaccggata cccgcctgac gcgggttttt tacggaaggg gggcaaaaat
     3061 gcctaatccg ctttacagac agcatatcat ctccatttcg gatttgtcgc gcgaacagtt
     3121 ggaatgcctg cttcagacgg cattgaagct gaaggcgcat ccgcgcggcg atttgttgga
     3181 aggcaaactt atcggttcgt gtttctttga accgtccacg cgcacgaggc tgtcgtttga
     3241 aacggcggtg cagcggctgg gcggcaaggt catcggcttt tcggacggcg cgaataccag
     3301 tgccaaaaaa ggcgagacgc ttgccgatac cgcccgcatc atttccggct acaccgacgc
     3361 aatcatccaa cgccacccca aagacggcgc ggcgcgcgtg gcagcggagt tttcgcgcgt
     3421 ccccgtcatc aacgccggcg acggcaccaa ccagcacccc agccagacct tgttggatct
     3481 ggtaacgatt tatgaaacgc aggggcgttt ggacaagctc aaaatcgcca tggcgggcga
     3541 cttgaaatac gggcgcaccg tgcattcgct ttgtcaggcg ttgaaacgct ggggctgcga
     3601 atttgccttt gtttcgccgc ccagcctcgc gatgcccgac tacattaccg aagagttgga
     3661 cgaagccggc tgccgatacc gtatcctcgg tagtttggaa gaagcggcgg aatgggcgga
     3721 tatcctgtat atgacccgcg tccagcgcga acgtttcgac gaacaggaat ttgccaaaat
     3781 ccaaggcaaa ttcaatctcg acgcatccat gcttgcccgc gccaaaccga acctgcgcgt
     3841 gctgcaccct ttgccgcgcg tggacgaaat ccatcccgat gtcgatgcta cgccgcatgc
     3901 ctattatttc gagcaggcga ccaacggcgt ttatgcgcgt atggcgatat tgtcgctggt
     3961 gttgaacgaa gaagtgtgag gaaccgatat ggaaaccccg aaactcagtg tcgaagccat
     4021 tgaaaaaggt acggttatcg accatattcc cgccggcagg gggctgacca tcctgcgcca
     4081 gttcaaactt ttgcactacg gcaacgcggt aaccgtgggc ttcaacctgc ccagcaaaac
     4141 ccaaggcagc aaagacatca tcaaaatcaa aggcgtgtgc ttggacgaca aagccgccga
     4201 ccgcctcgcc ctgttcgccc ccgaagcggt ggtcaatacc atcgacaatt tcaaggtcgt
     4261 gcagaagcgg catttgaacc tgcccgatga aattgccgaa gtgttccgtt gcccgaacac
     4321 gaattgcgcc ggccacggcg agccggtcaa aagccggttt tatgttaaaa aacacaacgg
     4381 gcagacgcgg ctgaaatgcc attactgcga aaaaacctac agccgggatt cggtggcgga
     4441 agcctgacgg attcccttaa accgagcggg cggcatttcg tctgccgctt gttttgccaa
     4501 tctgaaatgg aatgatgatg cacgcttctg tccaaagccg tttcgcaccg atactttatg
     4561 ttttgatttt ctttgccggt tttttgaccg cgcaaatttg gttcaatcag aaagcctata
     4621 ctgaagagct gcctccgctt ctgtccgcat tgtccgccgt cgcgctggtg tggctggcgt
     4681 gggcgttcgt gtcggcgcgt tcaaaggcta aggcggaaaa gttctaccgc gaaaaaatga
     4741 tacagaacga aagcatacac cccgtcctgc acgcttcttt gcaacacttg gaacacaagc
     4801 cgcaaatgct cgccctgctg gtcaaaaacc acggcaaagg gatggcggaa caggtcaggt
     4861 tcaaggcgga agtgctgccc gacgacgaag acgcgcgcac gattgccgcc gagttggcaa
     4921 aaatggatat gtttgcattg gggacggacg cggtcgcctc gggcgaaacc tatggacgcg
     4981 tgttcgccga tattttcgag ttgtcggcgg ctttggaagg gcgcgcgttc aaaggaatgt
     5041 tgaaactgac ggcggaatat aaaaacatct tcggcgatgc ctgccgttcg gaaacggcgt
     5101 tggatttggg cgcgctcaat caggcgttgc aggagatttc aaaaacatcg gaaaagtcca
     5161 aacggatatt ttattgaaga tggaaaaatg ccgtctgaaa cggaaggtgt ttcagacggc
     5221 atttttgtcg gatgattaat tattcggagc ggttgaagcc aaacttcacg cggctgcggc
     5281 cctgatccgg tatattgtcc aaatcgcgtc ccggattggc ggcggtgtcg cctacggaaa
     5341 tatcggagat gttttccaaa atgatggcgg acgacaggtg ttcggaggtg cggtaaacca
     5401 ttgccaagcc cacttcttcg gcaggagtgg aaatcagttc gacggtatcc ctgcttttga
     5461 aattgttgga gaggtcgacc tgcatcgttt tcttgcgttt gtagaggctc aaaaccgtgc
     5521 ctttgtccaa accgtccgcc tcgcctttgt cgatggtgat ggtttgaaac tggccggcaa
     5581 tccttgtgcc ttcaaacacg gaaacgattt tagcctgaac cgggcgggac ggttcgtgcg
     5641 gcatcatgtt gaagcggtcg gtgtcttccg gcattttcat caggtagtcg ccctgctgta
     5701 tttcggaaat ggcggtttcg accaccagcg gctgtatcga aggggtgcgc agcggggtaa
     5761 tcaaaggatg ggtgcgggta tggtattcgt tgtctttcgg ccgttctcca gcctgtttcg
     5821 agcgttgttc gaggacagag tcggtatagt cgagggagcg cacgatgccg ctgaatgcga
     5881 cttcctgccc gaggaattta cccgtatccg gatcggtgat gtttttattg attcggtagg
     5941 tcaggtagcg gcccggctct ttcaggcctt tggtgtaaac cctggtgcct ttggtgtaca
     6001 gcagcctgcc ttccgggccc gagagcaggc gcggcgcggc agcggtttct ttgcgggaaa
     6061 cgatttgcgg atgccgcata aagatgcggt agaagttgac atcgatggcg ggaataccgt
     6121 atccggacac ttccttatcc ggactcattt tgacgacggg gatgccgtct gtctgttcca
     6181 agccgaggcg cggttcgccg tcaacgtggc gcaacaccaa tacctggtcc ggataaatca
     6241 ggtcgggatt gtggatttga tcccggttcg cgtcccacag gcggctccat tgccacgggc
     6301 tgtacaggta tttgcccgaa atgccccaca gggtgtcgcc ctgtttgacc gtgtagcgtt
     6361 ccggcgcgtt cgggcgcacc tccaaatttg ccgccaaagt ttgtgttgag aatgccatac
     6421 ctgccgcgca gagcagggtt ataatacgac gttgcataac cgttcccctt atctgataaa
     6481 tttcggtttg tcttgcttga ttgggttgga aaaagcggcg gcagcccctc gggatgtgcc
     6541 gcgtgataaa aaatgttccg cattttaaca tcgaattatc cgcaccatca cggtaattat
     6601 gaaaaacagg cggcgtatcc gccgaaggaa agagaaaatt atggctttat tgaatatctt
     6661 gcaatatccc gacgagcgtc tgcacacggt ggcaaagcct gtcgaacaag tcgacgagcg
     6721 catccggaag ctgattgccg atatgtttga aacgatgtac gaatcgcgcg gcatcgggct
     6781 ggcggcgacg caggtcgatg tgcacgagcg cgtggtcgtg atggatttga ccgaagaccg
     6841 cagcgaaccg cgcgtgttca tcaaccccgt catcgttgaa aaagacggcg aaaccactta
     6901 cgaagagggc tgcctgtccg tgccgggcat ttacgacacc gtaacccgcg ccgaacgcgt
     6961 caaggtcgag gctttgaacg aaaaaggcga aaagttcacg ctggaggcgg acggcttgct
     7021 ggcgatttgc gtgcagcacg agttggacca cctgatgggc atcgtgtttg tcgaacgcct
     7081 gtcgcagctc aagcagggac gcatcaaaac caagctgaaa aaacgtcaga aacatacgat
     7141 ttgacccttt tgccgtgccg tctgaacgct gcaaagtttt cagacggcac ggtcttgtcc
     7201 gacaacctta cgcatacgca ggaatatgct atgaaagtca tctttgccgg cacgcccgat
     7261 tttgccgccg ccgccttaaa agccgttgcc gccgccggtt ttgaaatccc gctggtgctg
     7321 acccagcccg accgtccgaa agggcgcggt atgcaactga ccgccccgcc cgtcaaacaa
     7381 gccgcgctgg aactcggttt gcgcgtggca cagcccgaaa aactgcgtaa caacgccgaa
     7441 gccctgcaaa tgctcaaaga ggtcgaggca gacgtaatgg tggttgccgc ctacggtttg
     7501 attctgccgc aggaagtgtt ggatacgccg aaacacggct gcctcaacat ccacgcttcg
     7561 ctgttacccc gttggcgtgg cgcggcgccg attcaacgcg cgattgaagc cggcgatgcc
     7621 gagacaggcg tgtgtattat gcagatggac atcggtttgg acaccggcga tgtggtcagc
     7681 gaacaccgct acgccatcca accgaccgat accgccaacg aagtccacga cgcgctgatg
     7741 gaaatcggtg cggcggcggt tgttgccgat ttgcaacagc ttcaaagcaa aggccgtctg
     7801 aacgcggtca aacagcccga agaaggtgtt acttacgccc aaaaactaag caaagaagag
     7861 gcgcgtatcg attggagcaa aagcgcggcg gttatcgaac gcaaaatccg cgccttcaac
     7921 cccgtgcctg ccgcgtgggt tgagtatcag ggcaagccga tgaaaatccg gcgcgccgaa
     7981 gtggtggcgc aacaaggcgc ggcaggcgaa gtgttgtcct gttcggcgga cggtttggtc
     8041 gttgcctgcg gcgaaaacgc gctgaagatt accgaattgc agcctgccgg cggcaggcgg
     8101 atgaatatcg cggcgtttgc agcaggacgg catatcgaag cagggacgaa gctgtaaatc
     8161 ccttcagacg gcattccgat ccgcaaacgg gaatgccgcc tgaaaccatc agtcgaagaa
     8221 agcgaatcac ataatatgag tatggcactt gcccaaaaac ttgccgccga cagcattgcg
     8281 gcggttgccg aaggacgcaa ccttcaggac gtgttggcgc aaatccgcac cgcgcatccc
     8341 gaccttacgg cgcaggaaaa cggcgcgttg caggacatcg cctacggctg ccagcgttat
     8401 ttgggcagtt tgaaacatat gctcgcgcag atgctgaaaa agccgattgg caatccgcag
     8461 ctcgaaagtc tgcttttggc ggcgttgtac caactgcatt acacgcgcaa cgcgccgcat
     8521 gctgtggtca acgaagctgt tgaaagcatc gccaaaatcg gacgcgggca gtaccgttcg
     8581 tttgccaacg cggttttgcg ccgctttttg cgcgaacgcg acaagcttgc ggcttcctgt
     8641 aaaaaagacg atgtggcgaa acacaacctg ccgctgtggt gggtggctta cctgaaaaac
     8701 cattatccga aacactggca caacatcact gccgcgctgc aatcccatcc gccgatgacg
     8761 ttgcgcgtca accgccgaca cggcaatgcc gaaagctatt tggaaaaact ggtggcggaa
     8821 ggtatcgcgg ctaaggcgtt ggacgaatat gcggttacgt tggaagaagc cgtgccggta
     8881 aaccgtctgc ccggtttttc agacggcatt gtttcggtac aggacttcgg cgcgcagcag
     8941 gcggcgtatc tccttaaccc gaaagacggc gaacggattt tggacgcgtg cgccgcgccg
     9001 ggcggcaaga cggggcatat tttggaactg gcggattgcc gcgttaccgc cttggacatc
     9061 gatgcaggcc gtctgaagcg tgtggaggac aatatcgccc gtttgggctt tcagacggca
     9121 tcggcggcgt gtgccgatgc gcgggatttg gcggcgtggt atgatggaaa gcctttcgac
     9181 gcaatccttg ccgacgtgcc atgcaccgct tccggcgtgg cgcggcgcaa tcccgacgtg
     9241 aaatggctgc gccgtccgac cgacgcgcta aaaaccgccc gccagcagga agccctgctg
     9301 gacgcacttt ggcagacgct gaaacagggc ggcagaatgc tgcttgccac ctgttccgtg
     9361 ttcgtcgaag aaaacgacgg gcaattgcaa aaattcctca accgccatgc cgatgccgaa
     9421 ccgatcgaat cgcgggtgct tttaccgaac aaacaccaag atggttttta ttacgcgctt
     9481 attcaaaagc attaaacaat ggcttgtgtt gctgccgatg ctttccgttt tgccggacgc
     9541 ggcggcggag gggatagatg tgagccgcgc cgaagcgagg ataaccgacg gcgggcagct
     9601 ttccatcagc agccgcttcc aaaccgagct gcccgaccag ctccaacagg cgttgcgccg
     9661 gggcgtgccg ctcaacttta ccttaagctg gcagctttcc gccccgataa tcgcttctta
     9721 tcggtttaaa ttggggcaac tgattggcga tgacgacaat attgactaca aactgagttt
     9781 ccatccgctg accaaccgct accgcgttac cgtcggcgcg ttttcgacag actacgacac
     9841 tttgggtgcg gcattgcgtg cgaccggcgc ggttgccaac tggaaagtcc tgaacaaagg
     9901 cgcgctgtcc ggtgcggaag caggggaaac caaggcggaa atccgcctgc tgctgtccac
     9961 ttcaaaactg cccaagcctt ttcaaatcaa cgcattgact tctcaaaact ggcatttgga
    10021 ttcgggttgg aaacctttaa acatcatcgg gaacaaataa tgcgccgctt cctaccgatc
    10081 gcagccatat gcgccgtcgt cctgttgtat ggactgacgg cggcaaccgg cagcaccagt
    10141 tcgctggcgg attatttctg gtggatagtc gcattcagcg caatgctgct gctggtgttg
    10201 tccgccgttt tggcacgtta tgtcatattg ctgttgaaag acaggcgcga cggcgtattc
    10261 ggttcgcaga ttgccaaacg cctttccggg atgttcacgc tggtcgccgt attgcccggc
    10321 gtgtttctgt tcggcgtttc cgcacagttc atcaacggta cgattaattc gtggttcggc
    10381 aacgacaccc acgaagccct cgaacgcagc ctcaatttga gcaagtccgc attgaatctg
    10441 gcggcagaca atgccctcgg caatgccgtt cctgtgcaga tagacctcat cggcgcggcc
    10501 tccctatccg gcaatatggg cagggtgctg gaacattacg ccggcagctt tacccagttg
    10561 tccctttaca atgccgcaag cggcaaaatc gaaaaaagca tcaacccgca caagctcgat
    10621 cagccgtttc cggataaggc gcgttgggaa aaaatccaac aggcgggttc ggtcagggat
    10681 ttggaaagca taggcggcgt attgtacgcg cagggctggc tgtcggcggg tacgcacaac
    10741 gggcgcgatt acgccctgtt tttccgccag ccgattccca aagacgtggc agaggatgcc
    10801 gtcttaatcg aaaaggcgcg ggcgaaatat gccgagttga gttacagcaa aaaaggtttg
    10861 cagacctttt tcctggcaac cctgctgatt gcctcgctgc tgtcgatttt tcttgcactg
    10921 gtgatggcgc tgtatttcgc ccgccgtttc gtcgaacccg tcctatcgct tgccgagggg
    10981 gcgaaggcgg tggcgcaagg cgatttcagc caaacccgtc ctgttttacg caatgatgag
    11041 ttcgggcgtt tgaccagact gttcaaccat atgaccgagc agctttccat cgccaaagaa
    11101 gcagacgagc gcaacaggag gcgcgaagaa gccgccagac attatctcga atgcgtgctg
    11161 gaggggctga ccacgggcgt ggtggtgttt gacgaacaag gccgtctgaa aacctttaac
    11221 aaagcggcgg aacagatttt gggcttgtcg ctcgtgccgt tgtggggcag cagccggcac
    11281 ggttggcacg gcgtttcggc gcagcagtcc ctgcttgccg aagtgtttgc cgccatcggc
    11341 gcggcggcag gtacggacaa accggtccag gtggaatatg ccgcgccgga cgatgccaaa
    11401 atcctgctgg gcaaggcgac ggtattgccc gaagacaacg gcaacggcgt ggtgatggtg
    11461 attgacgaca tcaccgtatt gatacacgcg caaaaagaag cggcgtgggg cgaagtggcc
    11521 aaacgtctgg cacacgaaat ccgcaatccg ctcacgccca tccagctttc cgccgaacgg
    11581 ctggcgtgga aattgggcgg gaagctggac gagcaggacg cgcaaatcct gacgcgttcg
    11641 accgaaacca tcatcaaaca ggtggcggcg ttaaaagaaa tggtcgaagc attccgcaat
    11701 tatgcgcgcg cgccgtcgct caaattggaa aatcaggatt tgaacgcctt aatcggcgat
    11761 gtgttggcat tgtatgaggc cggcccgtgc cggtttgcgg cggagcttgc cggcgaaccg
    11821 ctgatgatgg cggcggatac gaccgctatg cggcaggtgc tgcacaatat tttcaaaaat
    11881 gccgccgaag cggcggaaga agccgatgtg cccgaagtca gggtaaaatc ggaagcgggg
    11941 caggacggac ggattgtcct gacggtttgc gacaacggca aggggttcgg caaggaaatg
    12001 ctgcacaacg ccttcgagcc gtatgtaacg gacaaaccgg cgggaacggg attgggtctg
    12061 cctgtggtga aaaaaatcat tgaagaacac ggcggccgca tcagcctgag caatcaggat
    12121 gcgggtggcg cgtgtgtcag aatcatcttg ccaaaaacgg taaaaactta tgcgtagcag
    12181 cgatatttta attgtagacg acgaaatcgg catccgcgac ctgctgtcgg aaatcctgca
    12241 ggacgaaggt tattcggtcg cattggcgga aaacgccgaa gaggcgcgca agctgcgcca
    12301 tcaggcgcgc cccgcgatgg tgctgctgga tatttggatg cctgattgcg acggcatcac
    12361 ccttttgaag gagtgggcga aaaacgggca gctcaatatg ccggtggtga tgatgagcgg
    12421 gcatgccagc atcgataccg ccgtggaagc caccaaaatc ggcgcgatcg attttttgga
    12481 aaaaccgatt tccctgcaaa agctgctgtc tgccgtcgaa aacgcgttga agtacggtgc
    12541 ggcgcaaacc gaaacggggc ctgtattcga caagctgggc aacagtgcgg cgattcagga
    12601 aatgaaccgt gaggtagggg ctgcggtgaa atgtgcctct cccgtacttt tgacgggcga
    12661 ggcgggttcg ccgtttgaaa cggtggcacg ctatttccat aaaaacggta cgccgtgggt
    12721 cagcccggca agggtcgaat atctgatcga tatgccgatg gaactgttgc agaaggcgga
    12781 gggcggcgtt ttgtatgtcg gcgacatcgc ccagtacagc cgcaacatcc aagccggtat
    12841 tgcctttatt gtcggaaagg cggaacaccg ccgcgtcagg gtggtcgcat cgggcagcag
    12901 ggcggcaggt tcagacggca ttgcctgcga ggaaaagctg gcggaactgc tgtcggaatc
    12961 ggtcgtccgt attccgccgc tgcgtatgca gcatgaagac attcccttcc tgatacaggg
    13021 gattgcctgc aatgtggcgg aaagccaaaa gattgcgcct gcctcattca gtgaagaggc
    13081 acttgccgca ttgacccgtt acgactggcc gggaaatttc gaccaactgc aaagcgtcgt
    13141 tgcaacgctg ttgttggagg cggacggaca ggaaatcggc gcaggggcgg tttcttccct
    13201 tttggggcag aatgtgcctg ccgagggagc ggaagatatg gtgggcgggt ttaatttcaa
    13261 cctgcccctg cgcgaattga gggaggaggt ggagcggcgt tatttcgagt accacatcgc
    13321 ccaagaaggt cagaatatga gcaaagtggc gcagaaagtc ggtttggaac gcacgcacct
    13381 ttaccgcaaa ctcaaacagc tcggcatcgg cgtttcgcgc cgggcggggg aaaaaaccga
    13441 agaataggcc cggacggccg gttttccggc tgcgggcttt tgttttcaga cggcatttgg
    13501 tgcaaatgcc gtctgaaatc gtaaggggac ggattttatg acagaggacg aacgtttcgc
    13561 gtggctgcaa ttggcgttta cgccctatat cggcgcggaa agcttcctgc tgctgctgcg
    13621 ccgtttcggc agcgcgtcaa acgccctgtc cgcaccggcg gaacaggtcg ccccggcggt
    13681 gcggcacaag caggcggccg aggcttggcg caatgcggaa aaacgcgcct cggcgcggca
    13741 ggcggcagaa gcggcgttgg aatgggaaat gcgggacgga tgccgtctga tgctgcttca
    13801 ggatgaagat ttccccgaaa tgctgacgca ggggctgacc gcgccgccgg ttttgttttt
    13861 gcgcggcaac gtgcggctgc tgcacaagcc ttccgccgcc atcgtcggca gccgccatgc
    13921 cacgccgcag gcgatgcgga ttgccaaaga tttcggtagg acgttgtctg ggaaaggcat
    13981 tcccgttgtg tcgggtatgg cttcgggcat cgataccgcc gcccatcagg gtgcgttgca
    14041 ggcagaaggc ggcaccatcg ccgtgtgggg gacgggcata gaccgcattt atccgccgtc
    14101 caataaaaac cttgcctatg aaatcgccga aaaagggctg attgtcagcg agttccccat
    14161 cggcacgcgc ccgtatgccg gcaactttcc gcgccgcaac cgcctgattg ccgcgctgtc
    14221 gcaggttacg ctggtggttg aagctgcgtt ggaatccggt tcgctgatta ccgccggcct
    14281 ggcggcagaa atggggcgcg aagtgatggc ggtacccggt tcgatagaca gtccacacag
    14341 caaaggctgc cacaaactga ttaaagacgg cgcaaaattg gtggaatgcc tggacgacat
    14401 cctgaacgaa tgcccggggc tattgcaaaa tacgggtgct tcatcatatt ctataaataa
    14461 ggatacgccc gacacgggac gccgcgccgt tcagacggca tacgccccgc cgcctgccgc
    14521 aaaaatgccg tctgaagcgg cggcaggcgg cacagcccac ggcggcatac tggataaaat
    14581 gggtttcgac cccgtccatc ccgacgtgct tgccgagcag cttgctatgc ctgccgcaga
    14641 tttgtatgcc gcactgttgg aattggaatt ggacggcagc gttgccgcaa tgcccggcgg
    14701 cagataccag cgtatccgaa cttgaacgca ctttatatta aggaacacga atgaccgaag
    14761 tcatcgccta cctcatcgaa catttccaag atttcgatac ctgtccgccg cccgaagact
    14821 tgggtatgct gcttgaagaa gcgggtttcg atacgatgga aatcggcaac accctgatga
    14881 tgatggaagt attgctcaac agctccgaat tttccgccga acctgccgac agcggcgcat
    14941 tgcgcgtgta cagcaaagaa gaaaccgaca acctgccgca ggaagtgatg gggctgatgc
    15001 agtatctgat tgaagaaaaa gccgtcagct gcgaacagcg ggaaatcatc atccacgcgc
    15061 tcatgcacat tccgggcgac gaaattaccg tagataccgc caaagtgctg accctgctgc
    15121 ttttatgggc aaacaagagc gagctgcccg tgttggtcgg cgacgagctg atgagcgcgc
    15181 ttttactcga caacaaaccc acgatgaact gaagcggctt cagacggccc gcccgaggcc
    15241 gtctgaaacg ccggcatcaa aaccaccatc cagagaacga caaatggcga aaaacctatt
    15301 aatcgtcgaa tccccgtcca aagccaaaac cctgaaaaaa tatttgggcg gcgattttga
    15361 aatccttgca tcctacggac acgtccgcga cctcgtcccc aaaagcggcg cggtcgatcc
    15421 cgacaacggc tttgcgatga aataccaatt gattagccgc aacagcaaac acgtcgatgc
    15481 catcgttgcc ggtgccaaag aagctgaaaa catctacctc gccaccgacc cggataggga
    15541 aggcgaagcc atttcctggc atcttttgga aatcctcaaa tccaaacgcg gcttgaaaaa
    15601 catcaagccg cagcgtgtcg tgttccacga aatcaccaaa aacgccgtgc tcgatgccgt
    15661 tgcccatccg cgcgaaatcg aaatggactt ggtcgatgca caacaagccc gccgtgcttt
    15721 ggactatttg gtcggcttta acctttcgcc attgttatgg aaaaaaatcc gccgaggttt
    15781 gagcgcgggg cgtgtgcaaa gccctgcttt gcgcctgatt tgtgagcgcg aaaacgaaat
    15841 ccgcgcgttt gaagcgcagg aatattggac ggtgcatctc gacagccaca aaggccgcag
    15901 caagttcacc gccaaactcg cccaatacaa cggcgcgaaa ctcgaacaat tcgacctgtc
    15961 gaacgaagcc gcgcaagccg acgtattgaa agaactcgaa ggcaaagaag ccgtcgttac
    16021 cgccattgaa aagaaaaagc gcagccgcaa tcctgccgcg ccgttcacca cttctaccat
    16081 gcagcaggat gccgtgcgca aactcggctt caccaccgac cgcaccatgc gtaccgccca
    16141 acagctttat gaaggtatag acgtagggca gggcgcgatc ggtctgatta cctatatgcg
    16201 taccgacagc gtgaatttgg cggatgaagc gttaaccgaa atccgccatt acattgaaaa
    16261 caaaatcggc aaagaatatc tgccgagtgc cgccaaacaa tacaaaacca aatccaaaaa
    16321 cgcccaagaa gcccacgaag ccatccgtcc gacttccgtg taccgcacgc ccgaaagcgt
    16381 caaacccttc ctgagcgcag accagttcaa actctatcaa atgatttggc agcgtaccgt
    16441 cgcctgtcag atgacgcctg ccaaattcga ccaaaccacc gtcgatatta ccgtcggcaa
    16501 aggcgtattc cgcgtaaccg gacaagtgca aaccttcgca ggcttcttga gcgtttatga
    16561 agaaagcagc gacgatgaag aaggcgaaga cagcaaaaaa ctgcccgaaa tgagcgaagg
    16621 cgataaattg cccgtggaca aactctacgg cgaacaacac tttaccactc cgccgccgcg
    16681 ctacaacgaa gccacgctgg ttaaagccct cgaagaatac ggcatcggcc gcccctcgac
    16741 ctacgccagc atcatctcca cgctcaaaga ccgcgaatac gttacccttg agcaaaaacg
    16801 cttcatgccc accgacacag gcgacatcgt caacaaattc ctgaccgaac acttcgccca
    16861 atacgtcgat taccacttca ccgccaaact cgaagaccag cttgacgaaa ttgccgacgg
    16921 caaacgccaa tggattccct tgatggacaa attctggaaa ccgttcatca aacaagtgga
    16981 agaaaaagaa ggcatcgaac gcgccaaatt taccacgcag gaacttgatg aaacctgccc
    17041 gaaatgcggc gaacacaaac tgcaaatcaa attcggcaaa atgggtcgtt ttgttgcgtg
    17101 tgccggttat cccgagtgca gctacacgcg caacgtcaac gaaactgccg aagaagccgc
    17161 cgagcgcatc gccaaagccg aggctgaaca agccgaactc gacggacgcg aatgtcctaa
    17221 atgtggtggt cgcctagtgt acaaatacag ccgaaccggc agcaaattca tcggctgcac
    17281 caactacccc aaatgcaaac acgtcgagcc gctggaaaaa ccgaaagata ccggcgtcca
    17341 gtgtccgcaa tgcaaaaaag gcaacctcgt cgagcgcaaa tcccgctacg gcaaactgtt
    17401 ttacagttgc agcacctatc ccgactgtaa ctacgccaca tggaacccac ccgttgcaga
    17461 aagctgtcca aaatgcgcat ggccggtttt gaccatcaaa accaccaaac gctggggtgt
    17521 agaaaaagtc tgcccacaaa aagaatgcgg ctggaaagaa cagattgaac cgcccgcgcc
    17581 gaaggagtaa gattaggttg gtttgaaaga gaaaaggtcg tctgaaaaat tttcagacga
    17641 cctttgcttt tctgtgattg gtttatttga atccgcgtgt tgttttaaag tccgataaaa
    17701 tccggttcat ttcaggcgca aacaaggcga tgtaatcgta agatagaccg cgactggcac
    17761 tgggatgggg aaagcagacg acttcgcaat cttcaaacga ttggaatttg acattgaaac
    17821 gtgtaccgtc aaattctttt tgcaccgtct ccagcggttt ggtctgctta ccgaccaact
    17881 gctcgaagcg tggcagtaca ttttggttgt tcagaaaatc cgccaacctg ctgcccatga
    17941 agaggatgac tttcggacgc agtttttcga tgtggtagag aaaattatcg atgtgctcgg
    18001 gttgtgtgaa cttgtctgga ttgtcgatag tgttgccctg tgtagcagcc cagttggttt
    18061 gaaccaggga tttttcaaat gcaccgccca atccattttc gtctaagggg tgtccccaca
    18121 tttcaaacca attttttatc gtattatcgt aacgccactt ttttgcctgc tctccgaaat
    18181 agagggattt gtttgcaaat gtatggtcga ttttgttttc agggagtttg tattcacctg
    18241 ctacataagc agcctcatcg gctttactcc aaccccattc atagccacaa atcattaagc
    18301 catgtttgtc gttgtagcct ttgaacaggc tgttgctcaa attcaaatcc ttcatcatga
    18361 actcttcctt ttaaaattta agagcgattg acttcaatgt ttttagatgg ggtggaaaaa
    18421 tccttgtgta ggcaacataa attcaataaa tttcttgata attcgaaacc tactaatagc
    18481 gcacctataa aagctttttc attacgttca gcatgacggt cacgtcgttc atatttttta
    18541 cgcttgctgt tccctgttat tacagctaag ccaagtgata tggcgagaat tgcccaaaca
    18601 attgtactta ataacaattt tccccatact atcaataagg aaagaaaaaa ccttttagta
    18661 ttagatcgat aggttataat ccatgcccat gaaaatgcaa taagagttat acataagata
    18721 caagctgaca ggatttcttt atttttaatt aaataactta gagcgatgag gatgacaggt
    18781 gtcaagaaaa taatagttac atcccgatag ctataaaaga aaactgccct attttgaatg
    18841 tggagatgtg cacagaatcc aatatagcta aggataatag ttaatataat aaaaaaagac
    18901 caccaagggt gaagagatag gaattccatg ttttcccttt ttttgtaaaa aggaaaaaat
    18961 cttatctaat agttaattgc ctaaccagcc agaagaagtt taaaatctat cccaataatt
    19021 caaccatcta tacagaaagt tcagcttatg gaaacccacg aaaaaatccg cctgatgcgc
    19081 gaattgaata aatggtccca ggaggatatg gcggaaaagc tggcgatgtc ggcaggcggg
    19141 tatgccaaaa tcgaacgagg cgaaacgcag ttgaatatcc cgcgtttgga gcagttggcg
    19201 cagattttca aaattgatat gtgggacttg ctcaaatcgg gcggcggcgg gatggtgttg
    19261 cagattaacg atgtggatac caacagcggg gaatttgcaa tctataccgc tcaggatgca
    19321 tcggtaaagc tggatttgtt aaaaatggaa ttaaaacact gtaaagaaat gttggaacac
    19381 aaagacaaag aaatcgagct gctccgcaag ctgaccgaaa ccgtttaaac agatatgccg
    19441 tctgaaaaaa gttttcagac ggcatattct ttgacaggtc ttgtataata ccgtttgaac
    19501 ttacaggttt ttgattatgg cggcaggcaa acataccaaa cacagtaacc gggtacgcat
    19561 tatcggcggg caatgccggg gcaggaaatt aagtttcaca tccgccgacg gactgcgtcc
    19621 gacacccgac agcgtgcgtg aaaagctgtt taactggctg ggacaggatt tgacgggtaa
    19681 aacggttttg gatctcttcg gaggcagcgg cgcactcggt atagaagccg cttcgcgcaa
    19741 cgccaaacgc gtgctgattt cggataacaa ccgccaaacc gtgcagacct tgcagaaaaa
    19801 cagtcgcgaa ctgggtttgg ggcaggtgca aatcgtctgt tcagacggca tcgcatattt
    19861 gaagaccgta tccgaacagt ttgatgttgt ctttcttgac ccgccgtttg catggcagga
    19921 ctggcaaatc ctgtttgatg ccttgaagcc gtgcctgaac ccccggtcat tcgtctatct
    19981 cgaggcgggt acgctgccgg atattcccga ttggctgacg gaatatagag aggggaaatc
    20041 ggggcagagt acatttgaat taagggtttt ccaagtggct gaataatatg cgctttgata
    20101 atcatttccg agttgtaaac attcgtttgc aaccgtccgg ttcaaaaaaa ccttgtgcta
    20161 taatccgcgc ccgcccggtt ttgataattt agtggaaaag gaaaagaaat gtcgcttttt
    20221 attaccgacg agtgcatcaa ctgcgacgta tgcgaacccg aatgccccaa tgatgccatc
    20281 tcgcaaggcg aggaaattta cgaaatcaac cccaacctct gcacgcagtg cgtcggacac
    20341 tacgatgaac cgcagtgcca gcaggtttgc ccggtggact gcatcctgat tgacgaagaa
    20401 catcccgaaa cgcatgacga gttgatggcg aaatacgaaa agattatcca gtttaaataa
    20461 attcttcttt aaaacatcaa attatgtctg ttttgaaata aaatcaaaaa aaaacttgac
    20521 ggaaaagcaa gccgctaata aactaacgtt ctcttttgga gggattcccg agcggtcaaa
    20581 gggggcagac tgtaaatctg ttgcgaaagc ttcgaaggtt cgaatccttc tccctccacc
    20641 aaaattctta cttggggcag tagcgagtaa tgcgggtgta gctcaatggt agagcagaag
    20701 ccttccaagc ttacggtgag ggttcgattc ccttcacccg ctccaaacaa ttaggcccat
    20761 gtagctcaga ggtagagcac tcccttggta agggagaggt cggcagttca aatctgccca
    20821 tgggcaccat ccctcgatta ttcatttctt taaggcttag atatatagga tattgccatg
    20881 gctaaggaaa aattcgaacg tagcaaaccg cacgtaaacg ttggcaccat cggtcacgtt
    20941 gaccatggta aaaccaccct gactgccgct ttgactacta ttttggctaa aaaattcggc
    21001 ggtgctgcaa aagcttacga ccaaatcgac aacgcacccg aagaaaaagc acgcggtatt
    21061 accattaaca cctcgcacgt ggaatacgaa accgaaaccc gccactacgc acacgtagac
    21121 tgcccggggc acgccgacta cgttaaaaac atgattaccg gtgccgcaca aatggacggt
    21181 gcaatcttgg tatgttccgc agccgacggc cctatgccgc aaacccgcga acacatcctg
    21241 ctggcccgcc aagtaggcgt accttacatc atcgtgttca tgaacaaatg cgacatggtc
    21301 gacgatgccg agctgttgga actggttgaa atggaaatcc gcgacctgct gtccagctac
    21361 gacttccccg gcgacgactg cccgattgta caaggttccg cactgaaagc cttggaaggc
    21421 gatgccgctt acgaagagaa aatcttcgaa ttggctgctg cattggacag ctacatcccg
    21481 actcccgagc gtgccgtgga caaacctttc ttgttgccta tcgaagacgt attctctatt
    21541 tccggtcgtg gtacagtagt aaccggccgt gtagagcgcg gtatcatcca cgttggtgac
    21601 gagattgaaa tcgtcggtct gaaagaaacc caaaaaacca cttgtaccgg tgttgaaatg
    21661 ttccgcaaac tgctggacga aggtcaggcg ggcgacaacg taggcgtatt gctgcgcggt
    21721 accaaacgtg aagacgtgga acgcggtcag gtattggcca aaccgggtac tatcactcct
    21781 cacaccaagt tcaaagcaga agtgtacgta ttgagcaaag aagagggtgg ccgccatacc
    21841 ccgttcttcg ccaactaccg tccgcaattc tacttccgta ccaccgacgt aaccggcgcg
    21901 gttactttgg aagaaggtgt ggaaatggta atgccgggtg agaacgtaac cattaccgta
    21961 gaactgattg cgcctatcgc tatggaagaa ggcctgcgct ttgcgattcg cgaaggcggc
    22021 cgtaccgtgg gtgccggcgt ggtttcttct gttatcgctt aagtttagag gccaatagct
    22081 caattggtag agtatcggtc tccaaaaccg agggttgggg gttcgagacc ctcttggcct
    22141 gccaaataaa aaattaaccg gccttgtgtc ggttaatttt tttgtatttg ttatttagta
    22201 aactctcttg ccatttacat ggattgagaa tagacagatg ctatgatgga taaataatat
    22261 gacagaacat acgcctgaaa aaaagaacgt taaagtggat caactggttg ttcaagataa
    22321 agaatctgca tctaattccg gtaaggaagg gttttttgca tatttctcaa attcttggtc
    22381 cgaattcaaa aaggtggttt ggcctaagcg tgaagatgct gtcagaatga ctgtatttgt
    22441 tatagtgttt gttgctgtgc tttctatatt tatctatgcg gcagatacag caatttcgtg
    22501 gttatttttt gatgtattgc tgagaaggga aggttgagat gtcgaaaaaa tggtatgttg
    22561 tacaggcgta ttcggggttt gagaagaatg tccaacgaat attggaagag cgcattgccc
    22621 gtgaggagat gggagattat ttcggacaaa ttctggtgcc tgtagagaaa gttgttgata
    22681 tccgcaatgg tcgtaagact attagtgaaa gaaagtcata tcctggttat gtgctagttg
    22741 agatggaaat gacagatgac tcttggcatc ttgtaaaaag caccccccgt gtttccggtt
    22801 ttattggagg gagggctaat agacctacgc cgattagtca gagagaggct gaaattattt
    22861 tacagcaggt tcagaccggc atagagaagc cgaaaccaaa agttgaattt gaggtcggtc
    22921 aacaggttcg tgtaaatgaa gggccgtttg cggattttaa cggggtggtt gaggaggtca
    22981 attatgaacg gaataagtta cgcgtgtctg ttcagatatt tggtagagaa acacccgttg
    23041 agctggagtt cagccaggtt gaaaagatta actgattttt atacttgaaa aaaaagcaat
    23101 aagaggatag aatcaaaaat taacttgggg agcggaaatg gttccgcgtc ttacccgttt
    23161 ttaggagtcc gttaagtggc aaagaaaatt atcggctata ttaaactgca aattcctgca
    23221 ggtaaagcca atccatctcc tccggttggt cctgctttgg gtcagcgcgg tttgaatatt
    23281 atggaatttt gtaaggcatt taatgctgca acccaaggta tggagcctgg cttaccgatt
    23341 ccagttgtga ttactgcatt tgcagataaa tcattcacat ttgtgatgaa aaccccgcca
    23401 gcttctatct tgttgaaaaa ggctgctggt ctgcaaaaag gtagttctaa tcctctgacc
    23461 aacaaagtgg gtaaattgac ccgtgctcag ttggaagaaa ttgctaaaac taaagagcct
    23521 gatttgactg ctgctgactt ggatgcggct gtccgtacta tagcaggttc tgctcgctca
    23581 atgggcttgg atgtagaggg tgttgtataa tggctaaagt atctaaacgc ttgaaagctc
    23641 ttcgctcttc tgtggaagcc aataaattat atgcaattga tgaagcaatt gctttggtaa
    23701 aaaaagcagc gactgctaaa tttgacgagt ctgttgacgt atctttcaac ttgggcgttg
    23761 atccgcgtaa atctgaccaa gttatccgtg gttcggtcgt tctgcctaaa ggcaccggta
    23821 agacaacccg tgtggctgta tttgctcaag gtgcaaatgc agaagctgct aaagaagctg
    23881 gtgcagatat cgtcggtttc gaagatttgg ctgctgaaat caaagcaggc aatctgaact
    23941 ttgacgttgt tattgcttct cccgatgcaa tgcgtattgt tggtcaattg ggtaccatct
    24001 tgggccctcg tggtctgatg ccaaacccta aagtaggtac ggttacccct aatgttgccg
    24061 aagcagtaaa aaatgcaaaa gcaggtcaag tgcagtaccg taccgacaaa gcaggtattg
    24121 ttcatgcaac gattggtcgt gcttcattcg ctgaagctga tttgaaagag aactttgatg
    24181 cgttgctgga tgctatcgtt aaagccaagc ctgctgccgc taaaggtcag tatctgaaaa
    24241 aagttgctgt gtccagcacc atgggtttgg gtattcgcgt tgatacatca agcgtaaata
    24301 actaatctta aggaatgttc aagcagtttg gttttctggg ctgcttgaat ttgggctact
    24361 taaaattaag tagatgtcca agaccgtagg gatcgtaaga tttaatcgta actgccctac
    24421 gcagacggta gtcctgaaac acattgcaag attgcttgta agatgtcttt ttaggttacc
    24481 gcgctggtgg gatatcgttt tggtatcctg tttataaaca gtgggaggta gaccttgagt
    24541 ctcaatattg aaaccaagaa agtggcggtc gaggaaatta gcgcggcaat tgctaatgct
    24601 caaaccctcg tagtcgctga atatcgcggt atcagtgttt ccagtatgac tgagcttcgt
    24661 gcgaatgcac gtaaagaagg cgtttatttg cgcgttctga aaaatacttt ggctcgtcgt
    24721 gcagtgcaag gtacttcatt tgcagaattg gccgatcaaa tggttggtcc gttggtttac
    24781 gctgcttctg aagatgctgt tgctgctgct aaagtgttgc accaattcgc gaaaaaagat
    24841 gacaaaattg tcgttaaagc cggttcttac aatggcgaag taatgaatgc tgctcaggtt
    24901 gctgagttgg cttctattcc gagccgcgaa gagctgttgt ccaagctgtt gttcgttatg
    24961 caagctcctg tatcgggctt tgcgcgcggt ttggctgctt tggcagagaa aaaagccggc
    25021 gaagaagccg cttaatcgat tttgtttctg ttaatcaatt attttttaat acaatatttg
    25081 gagtaaaata gcatggctat tactaaagaa gacattttgg aagcagttgg ttctttgacc
    25141 gtaatggaat tgaacgactt ggttaaagct tttgaagaaa aatttggtgt ttctgctgct
    25201 gctgttgcag ttgcaggtcc tgctggtgcc ggtgctgccg atgctgaaga aaaaaccgaa
    25261 tttgatgtcg ttttggcttc tgccggcgat caaaaagtcg gcgtgattaa agttgtccgt
    25321 gcaattaccg gtttgggtct gaaagaagct aaagacatcg ttgacggcgc acctaaaacc
    25381 attaaagagg gtgtttctaa agctgaagcc gaagacatcc aaaaacaact ggaagaagca
    25441 ggcgctaaag tcgaaatcaa ataatttgat gcttcttatg aaggctggca gttttctgcc
    25501 agccttattt tgcttcttaa aataaacatc aagtattgtt tacatttatt tgcacagttt
    25561 ttatcaagtc attgcaaata aatgtaaata tcagattgat gcgtgccgtt gctttcagac
    25621 ggcctattat tgaaaattac ttttcggagt gtatatgagc tattcgttta ccgagaaaaa
    25681 acgtatccgt aagagttttg caaagcggga aaatgttttg gaagttcctt tcctgctggc
    25741 aacccaaatt gattcttatg cgaaattttt gcaactggaa aatgcttttg acaaacgtac
    25801 cgatgacggt ctgcaggcag catttaattc tattttcccg attgtgagcc ataacggtta
    25861 tgcgcgattg gagtttgtgc attacacatt gggcgagcct ttgttcgata ttcccgaatg
    25921 tcagttgcgc ggaatcactt atgcagcccc cttgcgcgcg cgtatccgtt tggtgatttt
    25981 ggataaggaa gcatctaaac cgacggtaaa agaagttcgt gaaaacgaag tgtatatggg
    26041 cgaaattccg ttgatgaccc cgagcggttc ttttgtgatt aacggcacag agcgtgtgat
    26101 tgtttcccag ttgcaccgtt cgcccggcgt gtttttcgag catgacaaag gtaagacgca
    26161 ctcttccggc aaattgttat tctccgcccg catcattccc taccgtggtt catggttgga
    26221 ttttgaattt gatccgaaag atttgctgta tttccgtatc gaccgccgcc gtaaaatgcc
    26281 ggtaacgatt ttgttgaagg ctttgggcta caacaatgag caaatcttgg atattttcta
    26341 cgacaaagaa acgttctatt tgtcttcaaa cggtgttcaa accgatttgg tcgcaggccg
    26401 tctgaaaggc gaaactgcca aggtcgatat cttggataaa gaaggcaatg tattggttgc
    26461 caaaggtaag cgcattactg cgaaaaatat ccgtgatatt accaatgcag gcctgacccg
    26521 tttggatgta gaaccagaaa gcctgctggg caaagcattg gctgccgatc tgattgattc
    26581 ggaaaccggc gaggtattgg cttctgccaa tgatgaaatt acagaagagt tgttggccaa
    26641 atttgatatc aacggcgtaa aagaaattac gaccctttac atcaatgagt tggatcaggg
    26701 tgcttatatc tccaatacct tgcgtacgga tgagactgcc ggccggcagg cggctcgtgt
    26761 tgcgatttac cgtatgatgc gtccgggcga accgcccact gaagaggcgg tcgagcaatt
    26821 gtttaaccgc ttgttcttca gtgaagacag ctacgatttg tcccgcgtag gccgtatgaa
    26881 atttaatacg cgcacatacg aacaaaaact gtccgaagcc caacaaaact cttggtacgg
    26941 tcgcctgctg aacgaaacgt ttgccggtgc tgccgacaaa ggcggttatg tcctgagcgt
    27001 cgaagatatt gtcgcctcga ttgcgacttt ggtcgagttg cgtaacggcc atggcgaagt
    27061 ggacgatatc gatcacttgg gcaaccgccg agtacgttcg gtaggcgagc tgactgaaaa
    27121 ccaattccgt agcggtttgg cccgtgtgga acgtgccgta aaagaacgtt tgaatcaggc
    27181 ggaatcagaa aacttgatgc cgcacgattt gattaatgca aaacctgttt ctgccgctat
    27241 taaagaattc ttcggctcca gccaattgag tcagtttatg gatcagacca atcccttgtc
    27301 tgaagtaacc cataaacgcc gcgtatccgc attgggtccg ggcggtttga cccgcgaacg
    27361 tgcaggattt gaggtgcggg acgtgcatcc gacccactac ggtcgcgtat gtccgattga
    27421 aacgcctgaa ggtccgaaca tcggtttgat caactcattg tccgtttatg cgcgcaccaa
    27481 tgattacggt ttcttggaaa cgccttaccg ccgcgttatc gacggcaaag taaccgagga
    27541 aatcgattac ttgtctgcca tcgaagaagg ccgctatgtg attgcacagg cgaatgccga
    27601 tttggattca gatggcaatc tgattggcga tttggttacc tgtcgtgaaa aaggcgaaac
    27661 cattatggca acgcccgacc gcgtccaata tatggacgtg gcaactggtc aagtggtatc
    27721 cgttgcagca tccctgattc cattcttgga acatgatgac gcgaaccgcg cattgatggg
    27781 tgccaacatg caacgtcagg cagtgccttg cttgcgtcct gaaaaaccga tggtcggtac
    27841 cggtatcgag cgttccgttg ccgttgactc tgctactgca atcgttgccc gccgaggcgg
    27901 cgtggtcgag tatgtcgatg ccaaccgcgt tgtgatccgt gtccatgacg acgaagcgac
    27961 tgccggtgaa gtgggtgtcg atatttacaa cttggttaaa ttcacccgtt ccaaccagtc
    28021 taccaatatc aatcagcgtc ctgccgtcaa agccggcgat gttttgcaac gcggcgattt
    28081 ggtggccgac ggcgcgtcca ccgatttggg cgaattggct ttgggtcaaa atatgaccat
    28141 cgccttcatg ccgtggaacg gttacaacta cgaagactcg attctgattt ccgaaaaagt
    28201 ggctgcggac gaccgctata cttcgattca cattgaggaa ttgaatgtcg ttgcccgcga
    28261 tactaagctg ggtgcggaag acattacccg cgatattccg aacttgtccg agcgtatgca
    28321 aaaccgtttg gacgaatccg gtatcgttta catcggtgcg gaagtagaag ccggcgatgt
    28381 gttggtaggc aaggtaacgc ctaaaggcga aacccagctg acgccggaag aaaaactgct
    28441 gcgcgccatc ttcggtgaaa aagcatctga cgtaaaagat acttcattgc gtatgcctac
    28501 cggcatgagc ggtaccgtta tcgacgttca agtcttcact cgtgaaggta ttcaacgcga
    28561 caaacgcgcc caatccatta tcgattccga gttgaaacgc taccgtttgg atttgaacga
    28621 ccaattgcgt attttcgaca acgacgcatt cgaccgtatc gaacgtatga ttgtgggtca
    28681 aaaagccaat ggtggtccga tgaagctggc taaaggcagc gaaattacta ccgaatattt
    28741 ggcgggtctg ccgagcaggc acgattggtt cgatatccgt ctgaccgatg aagatttggc
    28801 caagcagttg gaactgatta aagtgagcct gcaacaaaaa cgcgaagaag cggacgagtt
    28861 atacgaaatc aagaagaaaa aactgaccca aggcgacgaa ttgcaacccg gcgtacaaaa
    28921 aatggtgaaa gtttttatcg ccatcaaacg ccgtctgcaa gccggcgaca aaatggcggg
    28981 ccgccacggt aacaaaggcg tggtatcgcg cattctgcca gtggaagaca tgccttacat
    29041 ggcggacggc cgtccggtag acatcgtact gaacccattg ggcgtacctt cccgtatgaa
    29101 catcggtcag attttggaag ttcacttggg ttgggcagca aaaggtatcg gcgagcgtat
    29161 cgaccgtatg ctgaaagagc aacgcaaagc aggcgagttg cgcgagttct tgaacaaact
    29221 ctacaacggc agcggcaaga aagaagattt ggatgccctg actgatgaag aaatcatcga
    29281 actggcctcc aacctgcgta aaggtgcatc tttcgcctct cctgtattcg acggtgcgaa
    29341 agagtctgaa atccgcgaaa tgttgaactt ggcttatccg agcgacgatc ctgaggttga
    29401 aaaactgggc ttcaacgaca gtaaaaccca aatcacgctg tatgacggcc gttcaggcga
    29461 agcatttgac cgcaaggtta cagtcggtgt gatgcactat ctgaaactgc accacttggt
    29521 tgacgaaaaa atgcacgcgc gttctaccgg tccgtacagt ctggttaccc agcagccttt
    29581 gggcggtaaa gcccagttcg gcggccaacg tttcggcgag atggaggttt gggcattgga
    29641 agcatacggc gcggcataca cgctgcaaga gatgctgact gtgaagtctg acgacgtgaa
    29701 cggccgtacc aaaatgtacg aaaacatcgt caaaggcgaa cacaaaatcg atgccggtat
    29761 gcccgagtcc ttcaacgtat tggtcaaaga gattcgctca ctgggcttgg atatcgattt
    29821 ggaacgttac taaacaaaag ttttcagacg gcctttcagg gtcgtctgaa aaagtggttt
    29881 cagaataaga atgaagcaat cggcatttag gccgtctgaa atcaaaagta ccgtttcccc
    29941 atatcgaaaa tccgccatgc ggtaaaaata cttccttcaa ggagcaaaaa tgaatttgtt
    30001 gaacttattt aatccgttgc aaactgccgg catggaagaa gagtttgatg ccattaaaat
    30061 cggtattgcc tctcccgaaa ccatccgctc atggtcttat ggcgaagtca aaaaacctga
    30121 aaccatcaac taccgtacgt tcaaacctga gcgcgacggt ttgttctgtg ccaaaatctt
    30181 tggcccggtc aaagactacg aatgcttgtg cggaaaatac aaacgcttga aatttaaagg
    30241 cgtaacgtgt gaaaaatgcg gcgtggaagt aaccctgtcc aaagtgcgcc gcgaacgcat
    30301 gggtcatatc gaattggctg cgcccgtcgc acatatttgg ttcttaaaat ccctgccttc
    30361 ccgcttgggt atggtgttag acatgacttt gcgcgacatc gagcgcgtat tgtactttga
    30421 agcatttgtg gtaaccgatc ccggtatgac tccgctgcaa cgccgccaat tgctgactga
    30481 agacgattac tacaacaagc tggacgaata cggcgacgac ttcgatgcca aaatgggtgc
    30541 agaaggtatc cgcgaattgt tgcgtaccct gaatgtagcg ggcgaaatcg aaatcctgcg
    30601 ccaagagttg gaatcgactg gttccgacac caaaatcaaa aaaatcgcca aacgcttgaa
    30661 agtattggaa gccttccatc gttccggtat gaaactggaa tggatgatta tggatgtgct
    30721 gccggtattg ccgcctgatt tgcgtccgtt ggttccattg gatggtggtc gttttgccac
    30781 ttccgatttg aacgatttgt accgccgcgt tattaaccgt aacaaccgtc tgaaacgtct
    30841 gttggaactg cacgcgcctg acatcatcgt ccgcaacgaa aaacgtatgt tgcaagaagc
    30901 ggtcgactcg ctgttggata acggccgtcg cggtaaagcc atgaccggcg ccaacaaacg
    30961 tccgctgaaa tcattggctg acatgattaa aggtaaaggc ggccgcttcc gtcaaaacct
    31021 gctgggtaaa cgtgtggact actctggtcg ttccgtgatt accgtaggcc cgtacctgcg
    31081 tctgcaccaa tgcggtctgc cgaaaaaaat ggctttggaa ctgttcaaac cgttcatttt
    31141 ccacaaattg gaaaaacaag gtttggcctc taccgttaaa gcagcgaaaa aattggtaga
    31201 acaagaagtg ccggaagtat gggacatctt ggaagaagtc attcgcgaac atccgattat
    31261 gctgaaccgt gcgccgaccc tgcaccgttt gggtattcaa gcgttcgaac ctatcctgat
    31321 tgaaggtaaa gcgattcagt tgcacccatt ggtgtgtgcc gcatttaacg ccgactttga
    31381 cggtgaccaa atggctgtac acgtcccatt gagcttggaa gcacaaatgg aggcacgcac
    31441 gctgatgctg gcttcaaaca acgtattgtc tccggccaac ggcgaaccga ttatcgtacc
    31501 ttcccaagac atcgtattgg gcctgtacta catgacccgc gaccgtatca atgccaaagg
    31561 cgagggcagc ctgtttgccg atgtgaaaga agtgcatcgc gcataccata ccaaacaggt
    31621 tgaactgggt acgaaaatta ccgtacgtct gcgcgaatgg gtgaaaaacg aagcaggtga
    31681 gtttgagcct gtcgttaacc gttacgaaac aaccgtcggc cgtgcattgt tgagcgaaat
    31741 cctgccgaaa ggcctgccgt ttgaatatgt caacaaagcg ttgaagaaaa aagaaatttc
    31801 taaactgatt aacgcatcgt tccgcctgtg cggcttgcgc gatacggtta tctttgctga
    31861 ccacctgatg tacaccggtt tcggatttgc ggcaaaaggc ggtatttcca ttgccgttga
    31921 cgatatggaa attccaaaag aaaaagcggc cttgctggct gaagccaatg ccgaggttaa
    31981 agaaatcgaa gaccaatacc gtcaaggttt ggttaccaac ggcgaacgct acaacaaggt
    32041 ggtcgatatt tggggtcgtg ccggcgataa gattgctaaa gcgatgatgg acaacttgtc
    32101 caaacaaaaa gttatcgacc gtgacggcaa cgaagtcgat caagagtcat tcaactccat
    32161 ttatatgatg gcggactccg gtgcccgtgg ttctgcagct cagattaaac agttgtccgg
    32221 tatgcgtggc ttgatggcta aacctgacgg ctcgattatt gaaacgccga ttacctcaaa
    32281 cttccgtgaa ggtctgaccg tattgcaata ctttattgcg acccacggtg cgcgtaaggg
    32341 tttggcggat accgcattga aaaccgcgaa ctccggttac ctgactcgtc gtctggtaga
    32401 tgtaactcaa gatttggtcg ttgttgaaga cgattgcggt acttcagacg gctttgtcat
    32461 gaaggcagtg gtacaaggcg gtgatgtgat tgaagcattg cgcgatcgta ttttgggtcg
    32521 tgttaccgcg tctgacgttg tcgatccgtc aagtggcgaa accttggttg aagccggtac
    32581 gttgctgact gaaaaactgg tggatatgat cgaccaatcc ggtgtcgatg aagtcaaagt
    32641 ccgtacgccg attacttgta aaacccgtca cggcctgtgt gcacactgtt acggtcgtga
    32701 cttggcacgc ggcaaactgg ttaacgccgg tgaggcagtc ggtgtgattg ctgcacaatc
    32761 cattggcgaa ccgggtaccc agttgaccat gcgtacgttc cacatcggtg gtgcggcatc
    32821 ccgtgcggca gcagccagcc aagtggaagc caaatccaac ggtacggcac gattcagtag
    32881 ccagatgcgc tacgttgcca acaacaaagg cgagttggtt gtcatcggcc gttcttgtga
    32941 agtcgtgatt cacgacgata tcggccgtga acgcgaacgc cacaaagtac cttacggtgc
    33001 catcctgctg gtacaagacg gtatggccat taaagccggt caaaccttgg caacctggga
    33061 tccgcatacc cgtccgatga tcaccgaaca tgcaggtatg gtgaaatttg agaacgtgga
    33121 agagggtgtt accgttgcga aacaaactga cgatgtaacc ggtttgtcca ctctagtagt
    33181 gattgacggt aaacgtcgtt cctctagtgc ttctaaactg ctgcgtccga ctgtaaaatt
    33241 gttggatgaa aacggcgtgg aaatctgtat tcccggtacg tccactccgg tttcgatggc
    33301 attccccgtt ggtgcggtga ttaccgtacg cgaaggtcag gaaatcggta aaggcgacgt
    33361 attggcgcgt atcccgcaag cctcttccaa aacccgcgac attaccggtg gtctgccgcg
    33421 cgttgccgaa ttgtttgaag cacgcgtgcc gaaagatgcc ggtatgttgg cggaaattac
    33481 cggtaccgtt tccttcggca aagagaccaa aggcaagcaa cgtctgattg ttactgacgt
    33541 ggacggtgta gcatacgaga ccttgatttc caaagagaaa caaattctgg tacacgacgg
    33601 tcaagtggta aaccgcggtg aaaccatcgt ggacggagct gttgatccgc atgatattct
    33661 gcgtttgcaa ggtatcgaag cactggcacg ctacattgtc caagaggtgc aagaggttta
    33721 ccgtctgcaa ggtgtgaaga tttctgataa acacatcgaa gtcatcatcc gtcaaatgtt
    33781 gcgccgtgtg aacattgcgg atgccggcga aaccgggttc attaccggag agcaggtcga
    33841 acgcggcgat gtgatggcgg ccaatgaaaa agctttggaa gaaggcaaag agccggcacg
    33901 ttacgaaaac gtattgctgg gtattaccaa agcctccctg tccaccgaca gcttcatttc
    33961 tgccgcatcg ttccaagaaa cgacccgtgt tctgaccgaa gccgcgatta tgggcaaaca
    34021 agacgaactg cgcggtttaa aagagaacgt catcgtcggc cgcctgattc ctgccggtac
    34081 cggtttgact tatcaccgca gccgtcatca gcaatggcaa ggggtagagc aggagactgc
    34141 tgaaacccaa gtaacggatg aataatcttc ggcgcgttta ttcaatacaa aaccgcaagc
    34201 tttaagcttg cggtttttct ttgtccgatt aaggcaaaaa caagcgtttt cgtcattttg
    34261 aggcgtgtgg attattcctt aggtattttc gggccggaga ccaacgaggt ggcgggtgtc
    34321 gtcggtacgt ccggagacca aaataacttt gccagggatg ttggtttcgg cggtcaaaaa
    34381 aagtagcgtc ttaatgtttt ccatttaaac aaatgtcgtc tgaaacttca gacggcattt
    34441 cctttaaaaa ataaatatga aacccagaaa tctctttttt gcaggctgcc tgctgacttc
    34501 ggcgacgttt gccgaggata tcggcgtacc tgtcgaactg attaacgtcg gtaatcggat
    34561 tgcgatgccg tctgaagggg aaagcctcgc cctcctgccg tttgccgagg atgtaccgcc
    34621 ggttcgcgat gcaatgccgt ctgaagttcc taaaagcgcg gcaggcggcg atgttcgggg
    34681 taaccggatg ggaatgccga ttaacatcgg atgagcgcgg ctttatggca taaaaaactg
    34741 tcgtggaaag gatttacacc ccaaataaat ttccgttaca acaagatcaa cagcaatatg
    34801 cccgcctttt attcgcgcag cggcaaggaa cggtttgtca gtatagaaaa aacgtattga
    34861 cagtattttc ttcagtcgtc cgactgattg tgagggatgt cggtaaatat ttatcggcaa
    34921 acaagaaaat catctttctt cttgtcgtta tgcttgactg tatacttgca ataaaaatat
    34981 aattccactc ttgccgacat ggtgtcggca agtatttaac tcaacaggac gagaaaatat
    35041 gccaactatc aaccaattgg tacgcaaagg ccgtcaaaag cccgtgtacg taaacaaagt
    35101 gcccgcactg gaagcctgcc cgcaaaaacg cggcgtgtgc acccgtgtat acacgactac
    35161 ccctaaaaaa cctaactctg cattgcgtaa agtatgtaaa gtccgcctga ccaacggttt
    35221 tgaagtcatt tcatacatcg gcggcgaagg ccacaacctg caagagcaca gtgtcgtatt
    35281 gattcgcggc ggtcgtgtaa aagacttgcc aggtgtgcgt taccacactg tacgcggttc
    35341 tttggatact gcaggtgtta aagaccgtaa acaagcccgt tccaaatatg gtgctaagcg
    35401 tcctaaataa ttactgggac ttaaataggc acgtcggccg cctaagcaga acaacggccg
    35461 agtaagtgaa tactcaattg ggtattcatg ggaatagacc cgactgaata gattaaagga
    35521 aattaaaatg ccaagacgta gagaagtccc caagcgcgac gtactgccag atcctaaatt
    35581 cggcagcgtc gagttgacca aattcatgaa cgtattgatg attgacggta aaaaatccgt
    35641 tgccgagcgt atcgtttacg gtgcgttgga acagattgag aaaaaaaccg gcaaagtagc
    35701 aatcgaagta tttaacgaag ccattgcaaa cgccaaacct atcgtggaag tgaaaagccg
    35761 ccgtgtaggt ggtgcaaact accaagttcc tgttgaagtt cgtccttcac gccgtttggc
    35821 tttggcaatg cgctgggttc gcgatgcggc ccgcaaacgt ggtgagaaat ccatggacct
    35881 gcgtttggca ggcgaattga ttgatgcgtc cgaaggccgt ggcggtgcgt tgaaaaaacg
    35941 tgaagaagta caccgtatgg ctgaagccaa caaagcattc tctcacttcc gtttctaatt
    36001 ttgaaaggct aataaaatgg ctcgtaagac cccgatcagc ctgtaccgta acatcggtat
    36061 ttccgcccat atcgatgcgg gtaaaaccac gacgacagaa cgtattttgt tctatactgg
    36121 tttgacccac aagctgggcg aagtgcatga cggtgcggct actaccgact acatggaaca
    36181 agagcaagag cgcggtatta ccattacctc cgctgccgtt acttcctact ggtccggtat
    36241 ggcgaaacaa ttccccgagc accgcttcaa catcatcgac accccgggac acgttgactt
    36301 taccgtagag gtagagcgtt ctatgcgtgt attggacggc gcggtaatgg tttactgcgc
    36361 ggtgggcggt gttcaacccc aatctgaaac cgtatggcgg caagccaaca aataccaagt
    36421 gccgcgcttg gcgtttgtca ataaaatgga ccgtcagggt gccaacttct tccgcgttgt
    36481 cgagcaaatg aaaacccgtt tgcgcgcaaa ccctgtacct atcgtcattc cggttggtgc
    36541 ggaagacaac ttcagcggtg tggttgattt gttgaaaatg aaatccatca tttggaatga
    36601 agccgataaa ggtacaacct ttatctatgg cgatattcct gccgaattgg tcgaaactgc
    36661 cgaagaatgg cgtcaaaata tgattgaagc cgcagccgaa gccagcgaag aactgatgga
    36721 caaatactta ggcggcgacg agctgaccga agaagaaatc gtaggcgcgt tgcgtcaacg
    36781 tactttggca ggcgaaattc agcctatgct gtgtggttct gcatttaaaa acaaaggtgt
    36841 tcaacgtatg ttggacgcag ttgtagaatt gctgccagct cctaccgata ttcctccggt
    36901 tcaaggtgtc aacccgaata ccgaggaagc cgacagccgt caagccagcg atgaagagaa
    36961 attctctgca ttggcgttca aaatgttgaa cgacaaatac gtcggtcagc tgacctttat
    37021 ccgcgtttac tcaggcgtag taaaatccgg cgataccgta ttgaactccg taaaaggcac
    37081 tcgcgaacgt atcggtcgtt tggtacaaat gactgccgca gaccgtactg aaatcgaaga
    37141 agtacgcgcc ggcgacatcg cagccgctat tggtctgaaa gacgttacta ccggtgaaac
    37201 cttgtgtgcg gaaagcgcgc cgattatctt ggaacgtatg gaattccccg agccggtaat
    37261 ccatattgcc gttgagccga aaaccaaagc cgaccaagag aaaatgggta tcgccctgaa
    37321 ccgcttggct aaagaagacc cttctttccg tgtccgtaca gacgaagaat ccggtcaaac
    37381 cattatttcc ggtatgggtg agctgcactt ggaaattatt gttgaccgta tgaaacgcga
    37441 attcggtgtg gaagcaaata tcggtgcacc tcaagtggct taccgtgaaa ctatccgcaa
    37501 agccgttaaa gctgaataca aacacgcgaa acaatccggt ggtaaaggtc aatatggtca
    37561 cgttgtgatt gaaatggaac ctatggaacc ggggggcgaa ggttacgagt ttatcgatga
    37621 aattaaaggt ggcgtgattc ctcgcgaatt cattccgtct gtcgataaag gtatccgcga
    37681 tacattgcct aacggtatcg ttgccggcta tcctgtagtt gatgtacgta tccgtctggt
    37741 attcggttct taccatgatg tcgactcttc ccaattggca tttgaattgg ccgcttctca
    37801 agcgtttaaa gaaggtatgc gtcaagcatc tcctgccctg cttgagccga ttatggcagt
    37861 tgaagtggaa actccggaag aatacatggg cgacgtaatg ggcgacttga accgccgtcg
    37921 cggtgttgta ttgggtatgg atgatgacgg tatcggcggt aaaaaagtcc gtgccgaagt
    37981 acctctggca gaaatgttcg gttattcgac cgacctgcgt tctgcaaccc aaggccgcgc
    38041 tacttactct atggagttca agaaatattc tgaagctcct gcccacatag ctgctgctgt
    38101 aactgaagcc cgtaaaggct aatcaggcaa ataggtcgtc tgaaaggctg aaatgatttt
    38161 tcagacggca ttgttcttta atcgatcttt aatgtaaagg aattagctca tggctaagga
    38221 aaaattcgaa cgtagcaaac cgcacgtaaa cgttggcacc atcggtcacg ttgaccatgg
    38281 taaaaccacc ctgactgccg ctttgactac tattttggct aaaaaattcg gcggtgctgc
    38341 aaaagcttac gaccaaatcg acaacgcacc cgaagaaaaa gcacgcggta ttaccattaa
    38401 cacctcgcac gtagaatacg aaaccgaaac ccgccactac gcacacgtag actgcccggg
    38461 ccacgccgac tacgttaaaa acatgattac cggtgctgcc caaatggacg gcgcgatctt
    38521 ggtatgttcc gcagctgacg gtcctatgcc gcaaacccgc gaacacatcc tgttggctcg
    38581 ccaagtaggt gtaccttaca tcatcgtatt catgaacaaa tgcgacatgg tagacgatgc
    38641 cgagctgttg gaactggttg aaatggaaat tcgtgacttg ttgtcaagct acgacttccc
    38701 aggcgacgac tgcccaatcg tacaaggttc tgcactgaaa gctttggaag gtgatgctgc
    38761 ttacgaagaa aaaatcttcg aattggctgc tgccttggac agctacatcc caacacctga
    38821 gcgtgctgtg gacaaacctt tcttgttgcc tatcgaagac gtattctcta tctctggtcg
    38881 tggtacagta gtaaccggtc gcgtagagcg cggtatcatc cacgttggtg acgagatcga
    38941 aatcgtaggt ctgaaagaaa cccaaaaaac cacttgtacc ggcgttgaaa tgttccgcaa
    39001 actgctggac gaaggtcaag caggtgacaa cgtaggcgta ttgctgcgtg gtactaaacg
    39061 tgaagacgta gagcgtggtc aagtattggc taaaccgggt accatcactc ctcacaccaa
    39121 attcaaagca gaagtatacg tactgagcaa agaagagggt ggtcgtcaca ctccattttt
    39181 cgctaactac cgtccacaat tctacttccg tactaccgac gtaactggtg cagttacttt
    39241 ggaagaaggc gtagaaatgg taatgccagg tgagaacgta accattactg tagaactgat
    39301 tgcgccaatc gctatggaag aaggtctgcg ctttgcgatt cgcgaaggtg gccgtaccgt
    39361 aggtgcgggc gtggtttctt ctgtaatcgc ttaattgagg gatattgata aatggcaaac
    39421 caaaaaatcc gtatccgcct gaaagcttat gattacgccc tgattgaccg ttctgcacaa
    39481 gaaatcgttg aaactgccaa acgcactggt gctgttgtta aaggtccgat tcctttgcca
    39541 actaaaatcg aacgtttcaa cattctgcgt tctccgcacg tgaacaaaac ttcccgtgaa
    39601 caattggaaa tccgcaccca cttgcgcctg atggacatcg tggattggac cgacaaaact
    39661 accgatgcgc tgatgaagct ggatttgccg gccggtgttg atgtagaaat caaagtccaa
    39721 taattagggc tgttgaaaat ccccaagcaa ttaatgcttg gggatttttt atgttatgcc
    39781 gtctgaaaat atgcggcggc aaacagattt aaggcaaaac aacaaaatca aaaaacagtg
    39841 cggtcggcga aatcggctta ttttgtgcct caagaatttt cagacggcat tttttagtga
    39901 acaaaacagg atatttttta tccatagcct tccgaaggct tgcccccccc cgaagaaagg
    39961 tagaatcggg cttggtttgg gcagaagcat ccatctgatt ctaaggatgg atgatgtttt
    40021 attctcaagt aaataaagga aagatgatga gcttacttaa tgtctttaaa gatattttaa
    40081 acaacaaaga attgaattcg gaagaacggc atgatttgga gcgggcggtt aagatcttgc
    40141 aggatacgca tgtcaatatt ttgattaccg gagccaccgg ttgcggaaaa tcatccacca
    40201 tcaatgcctt gttcagcata gatcaggcaa cgggaaagag tgatggacta gtaaaggaag
    40261 tcgcaaaagt aggcgtgggt gtagatcctg aaactatgga tattgccaaa tatgaattgg
    40321 agaatatgac gatttgggat agtccgggct tgggcgacgg agagaaggta gaccaaaagc
    40381 accggcgcaa tatcatcaat aagctgctgg agaaagacag taacggcaat gccgtcatcg
    40441 acttggtttt ggtgctgctt gacggcggct ccagagactt ggggacatca tacgaactga
    40501 ttaacaatgt aatcatcaat acattgaaga agagtgggga aggccgtaca gaccggttgc
    40561 tggttgcgat taatcagtgt gatatggcga tgaaggggcg gaattgggat gcggaagccg
    40621 gccgtccgaa cgccgccttg gaaaactttt tggaggagaa agtgtgttcg acacgcaagc
    40681 gcattcagga agctaccggc gttacggtca atccgattta tttttcagca ggctttaaga
    40741 gtgaggatga ggagcagcgt ccctataaca taacccgcct gttgcgttac atcattacgg
    40801 ctatgccgtc tgaaaaacgg gtgattgtgg cagagaagat taatgtggaa gaaatcgaac
    40861 gagataccag ccgccgcaaa caggaggagc ggcaagaaat cgacagtagt atcggcagtg
    40921 cgctggagaa aatattgggg gctgcgggag gcgtggttaa agatgttgtt tcaacgatag
    40981 caagcggtgt cgggacgctg atcggcggtg ctgcaaaagc gattggaggg gcgattagca
    41041 cggttgcttc tttttttggc tggtaggagc gcatatgcat cgttaccgta tttctgaaat
    41101 cgggcaaaaa gtcacagcag ccggtttccg tcatttggat gtcttgcttg tcggtgcgac
    41161 aggtgtcggc aagtcctcaa ccttaaatgc cttgtttggc gaacaaaaag ccaaggtagg
    41221 cacaggcgtg gatcccgaaa cgcaattggt cgaaagctat atgctgaacg atgtgttgcg
    41281 gttttgggac agcgcaggtt tgggcgatgg gaaagaagcc gaccgcgccc atcggcaaaa
    41341 actgattgat gtcctgtcta aaacctatac tcattcggat gggcagtggg gctggataga
    41401 tttggtgttc gttatccttg acggcagctc ccgcgatttg ggtacggcct atgatttgtt
    41461 gagggatgtt atccttaaaa tgattgatcc ggatagggtt attgttgcaa tcaatcaggc
    41521 ggatatggcg atgaaaggac gttattggga taaggtgctg catcagccgc agccgaattt
    41581 gcaacagttt ctagacgaga aggccgaatc ggtacaaaaa aggattctgg aagcaaccgg
    41641 tttgcagatt tccaaacccg tttactattc ggcatacgaa aactaccatc tcaaagaaat
    41701 aatggatgca gtcatcaacc atatcccggt ttgtcggaga aagatgcata cgccgtgatg
    41761 gtcggatacc gactccttta aaagcatccg accctatgcc gtctgaagat tcaaaacgct
    41821 tcagacggca tattgaagat atttttgata tttttgttga tatttctttg acttgtcaga
    41881 tataatgtct gacttggtac attcgtacca agtttaactt tgtctgaaag acaggccaat
    41941 cgtagcctgt ccctttactt taaaaggaaa ataatcatga ctttaggtct ggttggacgc
    42001 aaagttggta tgacccgcgt gttcgacgaa cagggtgttt ctgttccggt aaccgttttg
    42061 gatatgtctg ccaaccgcgt tacacaagta aaatccaaag atactgacgg ctatactgcc
    42121 gttcaagtta cctttggtca gaaaaaagcc aatcgtgtca acaaagccga agccgggcac
    42181 tttgcaaaag caggtgttga agccggtcgc ggtttgattg agtttgcttt gactgaagaa
    42241 aaactggctg aattgaaagc tggtgacgaa atcaccgttt ctatgtttga agtcggtcaa
    42301 ctggtcgatg taaccggtac ctctaaaggt aaaggtttct ccggcacgat caaacgtcat
    42361 aacttcggtg cccaacgtac ttcccacggt aactcccgtt ctcaccgtgt tccaggctct
    42421 atcggtatgg cgcaagaccc gggtcgcgtg ttccccggta aacgcatggc cggccaatac
    42481 ggcaacacca aagcaactgt tcaaaaattg gaagttgtcc gtgttgatgc agaacgccaa
    42541 ctgctgttgg ttaagggtgc tgttccgggt gcggtcaaca gcgatgttgt agttcgtccc
    42601 agcgtgaaag taggtgcgta atggaattga aagtaattga cgctaaagga caagtttcag
    42661 gcagcctgtc tgtttctgat gctttgttcg cccgcgaata caatgaagcg ttggttcatc
    42721 agctggtaaa tgcctacttg gcaaacgccc gctctggtaa ccgtgctcaa aaaacccgtg
    42781 ccgaagtaaa acactcaacc aaaaaaccat ggcgtcaaaa aggtaccggc cgcgcccgtt
    42841 ccggtatgac ttcatctccg ctgtggcgta aaggtggtcg cgcgttcccg aacaaacccg
    42901 acgaaaactt cactcaaaaa gtaaaccgca aaatgtaccg tgccggtatg gcgactattc
    42961 tgtcccaatt gactcgtgac gagcgtttgt ttgcgattga ggcgttgact gctgaaactc
    43021 ctaaaaccaa agtttttgct gaacaagtga aaaatctggg tctggagcaa gtgttgtttg
    43081 taaccaaaca gctcgacgag aatgtttact tggcttcacg caacttgcca aacgtgttgg
    43141 ttttggaagc tcaacaagtt gatccttaca gcttgctgcg ttacaaaaaa gtaatcatca
    43201 ctaaagatgc agttgcacaa ttagaggagc aatgggtatg aatcaacaac gtttgactca
    43261 agtgattttg gcacctatcg tttctgaaaa aagcaacgta ttggctgaaa aacgcaacca
    43321 aatgacgttt aaagtcttgg caaatgcaac caaacctgaa atcaaagctg ctgttgagct
    43381 gctgtttggt gttcaagttg cttctgtaac taccgttaca actaaaggca aaactaagcg
    43441 ttttggtcgt actttgggcc gccgcagcga tgttaaaaaa gcttatgtaa gcttggctgc
    43501 cggtcaagag ttggatttgg aagccgctgc tgcagctgca gataaggaat aaacaaaatg
    43561 gcaatcgtta aaatgaagcc gacctctgca ggccgtcgcg gcatggttcg cgtggtaaca
    43621 gaaggtttgc acaaaggtgc accttatgcg cccttgcttg aaaagaaaaa ttctactgcc
    43681 ggccgtaaca ataatggtca tatcaccacc cgtcacaaag gcggcggtca taaacaccat
    43741 taccgtgttg tagactttaa acgtaacaaa gacggcattc ctgccaaagt agagcgtatc
    43801 gaatacgatc ctaaccgtac tgccttcatc gcactgttgt gctatgcaga cggcgagcgt
    43861 cgttacatca ttgctcctcg cggtattcaa gccggtgctg tattggtttc cggtgctgaa
    43921 gctgccatca aagtaggcaa caccctgccg attcgcaaca ttcccgttgg tacgactatc
    43981 cactgtatcg aaatgaaacc cggtaaaggt gctcaaatcg cacgttctgc cggtgcttct
    44041 gcggtattgc tggctaaaga aggcgcgtac gctcaagtcc gtctgcgctc tggcgaagtc
    44101 cgtaaaatca acgtaaattg ccgtgcgacc attggtgaag ttggtaacga agagcaaagc
    44161 ctgaaaaaaa tcggtaaagc cggtgccaac cgttggcgcg gtattcgtcc gaccgtacgc
    44221 ggtgttgtca tgaatcctgt cgatcacccg catggtggtg gtgaaggccg tacgggcgag
    44281 gcccgcgaac cggtcagccc atggggtact cctgctaaag gctaccgcac tcgtaataac
    44341 aaacgcacgg ataacatgat tgttcgtcgc cgttactcaa ataaaggtta atttagtatg
    44401 gctcgttcat tgaaaaaagg cccatatgta gacctgcatt tgctgaaaaa agtagatgct
    44461 gctcgcgcaa gcaacgacaa acgcccgatt aaaacctggt ctcgtcgttc taccattctg
    44521 cctgatttta tcggtctgac cattgctgtg cacaacggcc gcacccatgt gcctgtgttt
    44581 atcagcgaca atatggttgg tcataaatta ggcgaattct cattgacccg tacctttaaa
    44641 ggccacttgg ccgataaaaa ggctaaaaag aaataaggtg aatcatgaga gtaaatgcac
    44701 aacataaaaa tgcccgtatc tctgctcaaa aggctcgttt ggtagctgat ttgattcgtg
    44761 gtaaagacgt tgcccaagct ttgaatattt tggctttcag tcctaaaaaa ggtgccgagc
    44821 tgattaaaaa agtattggag tcagctattg ctaatgccga gcacaataac ggtgcggaca
    44881 ttgatgaact gaaagtggta actatctttg ttgacaaagg cccaagcttg aaacgttttc
    44941 aagctcgcgc caaaggtcgc ggtaaccgca tcgaaaaaca aacttgtcat atcaatgtga
    45001 cagtgggtaa ctaaggaaaa gctatgggac aaaagattaa ccctacaggc tttcgcctgg
    45061 cggtaactaa agactgggct tcaaaatggt ttgctaaaag caccgacttt tctactgttt
    45121 tgaagcagga tatcgatgtt cgcaattatt tgcgtcaaaa attggccaat gcttcggttg
    45181 gtcgagtggt tattgaacgc cctgcaaaat ctgcacgcat taccattcac tccgctcgtc
    45241 cgggtgtggt tataggtaaa aaaggtgagg atatcgaggt tttgaaacgt gacttgcaag
    45301 tcttgatggg tgtacctgtt catgtaaata ttgaagagat tcgccgtcct gagttggatg
    45361 ctcaaattat tgctgacggt attgcccagc agttggaaaa gcgcgttcaa ttccgtcgtg
    45421 ctatgaaacg agcaatgcaa aatgcaatgc gttctggtgc taaaggcatt aagattatga
    45481 cttcaggccg tctgaatggt gcggatattg cccgtagcga atggtatcgt gaaggtcgcg
    45541 tgccactgca tactttacgt gcaaatgtag attatgcaac cagcgaagcg cacaccacat
    45601 atggtgtatt gggtctgaaa gtttgggttt atacggaagg caatattaaa tcttccaaac
    45661 ctgaacatga gagtaaacaa agaaaggcag gtagacgtaa tgctgcagcc aactagactg
    45721 aaataccgta agcaacaaaa gggtcgcaat actggcatcg ctactcgcgg taataaggta
    45781 agtttcggtg agttcggctt gaaagccgta ggtcgtggtc gtttgactgc ccgtcaaatc
    45841 gaagctgctc gtcgtgcaat gacccgtcat atcaaacgtg gtggtcgtat ttggattcgt
    45901 gtattccctg ataaaccgat tactgaaaag cctattcaag ttcgtatggg tggcggtaaa
    45961 ggtaacgtgg aatattacat tgccgaaatt aaaccaggta aagtgttgta tgaaatggat
    46021 ggcgttccag aggaactggc tcgtgaagca ttcgagttgg ctgctgccaa attgcctatt
    46081 cctacaacct ttgtagtaag acaggtgggt caataatgaa agcaaatgaa ttgaaagaca
    46141 aatccgttga gcagttgaat gcagatttgt tggacttgtt gaaagctcag tttggcttac
    46201 gtatgcaaaa cgctaccggt caattaggca aaccaagtga attgaaacgt gtacgtcgcg
    46261 atattgctcg tattaaaacc gttttaactg aaaaaggtgc taagtaatga gcgaaactaa
    46321 aaatgttcgt actttgcaag gcaaagtagt aagcgacaaa atggataaaa ccgtaacagt
    46381 attggttgag cgtaaagtaa aacatccgct gtatggtaag attattcggt tatctactaa
    46441 aatccatgcc catgatgaaa ataatcaata tggaattggt gatgtggttg ttatatcgga
    46501 atcccgtcca ttgtcaaaaa ctaaatcttg ggttgtcagt gagctggttg agaaagcacg
    46561 ttctatttaa gaattaaagc aacgtgcttg gaatgggaaa cgaagtattg cagcaaattt
    46621 aatttgcgtg taaacttcgt ttcctgtctt tcagtttctt ctggaagttt cttccctttc
    46681 ggggtccaag actggtttac ttgaaccgca aggtttcatt taataagcag cggctttgct
    46741 gtaagttatc tgaaagtggt aaattaagtt ggttaattta aaggtaataa catgattcaa
    46801 atgcagacca tcttagatgt ggctgataac tctggtgcgc gtcgcgtaat gtgtatcaag
    46861 gtattgggcg gatctaagcg tcgctacgct tctgttggcg atattattaa agtggcagtt
    46921 aaagatgcgg ctccgcgtgg ccgtgtcaaa aaaggcgatg tatataatgc ggtagttgtt
    46981 cgtactgcta agggtgtacg tcgtcctgat ggtgcgttaa ttaaattcga taacaatgcc
    47041 gccgtgttac tgaataataa acttgaacct ttgggtactc gtatctttgg tccggtaacc
    47101 cgtgaattgc gtactgagcg atttatgaaa atcgtttcat tggcacctga agtattataa
    47161 ggaatggcac gatgaataaa atcattaaag gcgatagggt tgtagtaatt gctggtaagg
    47221 ataaaggtaa gcagggtcaa gtagttcgag tgttgggtga taaagttgtt gttgagggcg
    47281 ttaatgttgt aaaacgccat caaaaaccta atccaatgcg tggcattgag ggcggtatta
    47341 ttactaaaga aatgcctttg gatatttcta atatcgcaat cctgaatccg gaaactaata
    47401 aagcggaccg tgttggtatt aagctgattg aaaatgaagg caaagttaaa cgcgttcgtt
    47461 tcttcaaatc aaatggctct atcattgggg cataaggaga taacatggct cggttgagag
    47521 agttttataa agagacagtt gttcctgaat tggttaaaca atttggttac aaatcagtaa
    47581 tggaagtccc gcgtattgaa aaaattacct tgaatatggg tgtgggtgag gctgttgctg
    47641 ataaaaaagt tatggaacat gctgtttccg atttagagaa aattgccggt caaaaaccgg
    47701 ttgttactgt tgcccgtaaa tctatcgcag gttttaaaat ccgtgataac tatccggttg
    47761 gttgcaaagt aacattgcgt cgtgatcaaa tgtttgaatt cttggatcgt ttgattacta
    47821 ttgcattacc tcgcgtacgt gacttccgtg gtgtgagcgg taaatcattt gatggccgtg
    47881 gcaattacaa tatgggtgtt cgtgagcaaa ttatttttcc ggaaattgaa tacgataaaa
    47941 ttgatgcttt gcgtggtttg aatattacta ttactactac agcaaaaacc gatgaggaag
    48001 cgaaagcttt attgtcattg tttaaatttc cgttcaaagg ataatcatgg ctaagaaagc
    48061 acttattaat cgtgatctga aacgtcaagc tttggctaaa aaatatgcgg ctaaacgcgc
    48121 ggcaattaaa gcggtaatca atgattcgaa tgcaactgag gaagagcgtt ttgaggctcg
    48181 tttgaggttt caatccattc ctcgtaatgc ggcacctgtg cgtcaacgtc gtcgttgtgc
    48241 tttgacaggt cgccctcgtg gtactttccg taaatttggt ttgggtcgta ttaaaatccg
    48301 tgaaatcgcc atgcgtggcg aaattccggg tgttgttaaa gccagctggt aataggagta
    48361 attaagaatg agtatgcatg atcctatttc cgatatgttg actcgtatcc gcaatgcgca
    48421 acgtgctaat aaagcagcag ttgcaatgcc ttcttcaaaa ttaaagtgtg ctattgcaaa
    48481 ggtattgaaa gaagaaggat atattgagga cttcgcagtt tcatctgacg taaagtctat
    48541 attggaaatt caattaaaat actatgcagg tcgtcctgta attgaacaaa tcaagcgtgt
    48601 atctcgcccc ggtttgcgta tttataaagc gtctagtgag attccaagtg ttatgaatgg
    48661 cttgggtatt gctattgtta gtacttctaa aggtgtaatg actgatcgta aagcacgttc
    48721 tcaaggtgtt ggtggtgagt tgttatgcat tgtagcctag tggaggaaaa gaaatgtcac
    48781 gtgtcgcaaa aaacccagtg actgttcccg ctggtgtaga agtaaaattt ggaacagagg
    48841 cattagttat taagggtaag aacggtgaat tgtcttttcc tttgcattct gatgtagcca
    48901 ttgaatttaa tgatggcaaa ttgacttttg ttgcgaataa cagcagtaaa caagcaaatg
    48961 caatgtctgg tactgctcgc gcattagtca gcaatatggt taaaggtgtt tcagaaggtt
    49021 ttgagaaaag attgcaattg ataggtgtgg gttatcgtgc tcaagcacaa ggtaaaatct
    49081 tgaatctgtc tttgggtttt tctcatccga tcgtatatga aatgcctgaa ggtgtctccg
    49141 ttcaaactcc tagccaaaca gagattgttt taaccggctc ggataaacaa gttgttggtc
    49201 aagttgctgc tgagattcgt gcgttccgtg ctcctgagcc ttataaaggt aaaggtgttc
    49261 gctatgtagg cgaagtagtg gtgatgaaag aagccaagaa aaaataattg aggttcacta
    49321 atggataaac atacaacccg actccgtcgt gcacgcaaaa cccgtgctcg tattgcggac
    49381 ttgaaaatgg taagattatg tgtgttccga agcaataatc atatttatgc tcaagtaatt
    49441 agtgctgaag gtgataaagt attggctcaa gcctctacat tggaagctga ggtgcgcggt
    49501 agtctgaaat ctggaagcaa tgttgaagca gctgcaatag ttggtaaacg tatcgctgag
    49561 aaggctaaag cagcaggtgt agaaaaggtt gcttttgatc gttcaggttt ccaatatcac
    49621 ggtcgtgtga aggctttggc tgaagctgct cgtgaaaatg gtttaagctt ctaaatattt
    49681 ggagactttc agatggcaaa acatgaaatt gaagaacgcg gtgacggtct gattgaaaag
    49741 atggtcgcag ttaaccgtgt aaccaaagta gttaaaggtg gtcgcattat ggctttctct
    49801 gcgctaactg ttgttggtga tggagatggt cgtattggta tgggtaaagg taagtcaaaa
    49861 gaagtgcctg ttgcagtcca aaaggcgatg gatcaagcac gacgctctat gattaaggta
    49921 ccattaaaaa atggtacgat tcatcatgag gttattggtc gacatggtgc tactaaagta
    49981 tttatgcagc ctgctaaaga gggcagtggc gtaaaagccg gtggacctat gcgtttggtt
    50041 tttgatgcta tgggcattca taatatctcc gccaaagtgc acggatctac taacccatat
    50101 aatatcgtac gtgcaacatt agatggcttg tctaagttgc atactcctgc tgatatcgca
    50161 gccaaacgtg gcttgacagt ggaagacatt ttgggagtta accatggctg aacaaaaaaa
    50221 gattagggtt acattggtta aaagcctgat tggtacaatt gaatctcatc gtgcatgtgc
    50281 acgcggttta ggtttgcgtc gtcgcgagca tacggtagag gttttagata cccctgaaaa
    50341 ccgtggtatg attaataaaa tcagctactt gttgaaagtg gagtcttgat atgtttttga
    50401 atacaattca acctgctgtt ggtgctacgc atgctggtcg tcgtgttgga cgcggtattg
    50461 gtagtggtct tggcaaaacg ggtggtcgtg gtcataaagg tcaaaagagc cggtctggtg
    50521 ggtttcataa ggtgggtttc gagggtggtc aaatgccctt gcaacgacgc ctccctaaaa
    50581 gaggttttaa atctttaaca gcatcagcta atgcacagct tcgtttaagt gaactggaat
    50641 caattgctgt taatgagatt gatattttgg tcttaaagca agcgggtctg attgcatcta
    50701 cagtctctaa tgttaaagtt attgcttctg gtgaaatttc taaggcagtt gctttgaagg
    50761 gtattaaagt taccaaaggt gcgagagctg ctatcgaggc tgttggtggt aagattgaaa
    50821 tgtaaggttt aatattgtgg ctaatcaaca aacgtcatca ggttcatcca aatttggaga
    50881 tcttaagaaa cgtcttttgt ttctatttgg agcattgatt gtttttcgaa ttggtgccca
    50941 tatacccgta cctggagttg atgctgttgc tttagctaaa ttatacgaaa gcgctggaaa
    51001 cggcatcctg ggaatattga atatgttttc cggtgggtcg ttagagcgct ttagtatatt
    51061 tgcaatagga attatgccat atatttcagc ttctattatt gtacagctcg cttctgaaat
    51121 tttgccatca ttgaaggctt taaaaaaaga aggggaggct ggtagaaagg taattacgaa
    51181 atatactagg tatggtactg ttttgttagc aattcttcaa agtctaggtg ttgcatcttt
    51241 cgtatttcag caaggaattg ttgtaacaag ttcatttgag tttcatgttt ccacggtagt
    51301 ttctttggta acgggaacca tgtttcttat gtggcttggg gagcaaatta ctgaaagggg
    51361 tatcgggaac ggtatttctt taatcattac ggcaggtatt gcttcaggta ttccttcggg
    51421 tattgcaaag ctggttacac tgacgaacca aggttctatg agcatgctta cggcgttgtt
    51481 tattgtattt ggtgccttat tattaattta tttggttgta tactttgaaa gtgcacagcg
    51541 gaagattcct attcattatg caaaacgcca gtttaatggt agggcgggta gtcaaaatac
    51601 gcatatgcct ttcaagttga atatggctgg tgttattccc ccaatttttg cttccagtat
    51661 tattctattt ccatctactc ttttaggttg gtttggttcg gctgatacaa atagtgtttt
    51721 gcacaaaata gctggattgt tacaacacgg tcaattgctg tatatggctt tatttgcagc
    51781 gacagttatt ttcttttgtt atttttatac ggctttggtt tttagcccta aagaaatggc
    51841 agagaattta aaaaagagtg gtgcttttgt tcctgggatt agacctggtg agcagacctc
    51901 taggtattta gaaaaagttg tattacgttt gacattgttt ggagctcttt atattacaac
    51961 tatttgttta attccagagt tcttaactac ggttttaaat gtaccttttt atttgggtgg
    52021 cacgtctttg ttgattctag ttgttgtaac gatggatttt agtacacaaa taaattcgta
    52081 taggcttact caacagtatg ataagttaat gactcgttca gaaatgaaat cattttctcg
    52141 gaaatagaat tatggcgaaa gaagatacta tccaaatgca aggtgaaatt cttgaaactt
    52201 tacctaatgc aacatttaaa gtaaaacttg agaatgacca tattgtattg ggtcatattt
    52261 ctgggaagat gcggatgcat tacattcgta tttctccggg agataaggtc acagtagagc
    52321 tgacacctta tgatctaact agggctcgaa tcgttttcag agcaagataa accaataaaa
    52381 ggaaaataaa atgcgtgtac aaccatctgt taagaaaatt tgccgaaatt gcaagattat
    52441 tcgtcgaaat cgtgtagttc gtgtaatttg tactgatctc cgtcacaaac agcgtcaagg
    52501 ttaatggaat atttctttta atgtgattct gtgatatagt gacacacttt gccctaaaaa
    52561 ggaaaaaata tggctcgtat tgcaggggta aatatcccta ataacgcaca catcgtaatt
    52621 ggtcttcagg ctatttacgg tattggtgct actcgtgcta aattgatttg tgaggctgca
    52681 aatattgcgc ctgatactaa agcaaaagat ttggacgaga ctcaattaga tgctttgcgt
    52741 gaccaagttg ccaagtatga agtagaaggt gatttgcgtc gtgaggtaac tatgagtatc
    52801 aagcgattga tggacatggg ctgctatcgt ggcttccgtc atcgtcgcgg cttaccatgc
    52861 cgcggtcaac gcactcgtac aaatgcgcgt acccgcaaag gtccgcgtaa agcgattgct
    52921 ggtaagaaat aaattttaag gaattttatt aatggctaaa gcaaacacag cttcacgtgt
    52981 acgtaaaaaa gtacgtaaaa ccgtgagtga gggtattgtg cacgttcatg catctttcaa
    53041 caataccatc attacaatca ctgaccgtca aggcaatgcg ttgtcttggg ctacctctgg
    53101 cggcgctggt tttaaaggtt ctcgtaaaag tacaccattt gcagcacaag ttgcagcaga
    53161 agcagctggt aaagttgccc aagagtatgg cgttaaaaat ttagaggttc gtattaaagg
    53221 tccaggtcca ggtcgtgaat cctctgtacg tgctttgaat gctcttggtt tcaagattac
    53281 cagcattact gacgttaccc cgttgcctca taacggttgc cgtccgccta aaaaacgtcg
    53341 tatttaatat tggagtgatt tgaaacatgg cacgttatat tggccctaaa tgtaagttgg
    53401 cacgtcgcga aggtacggat ttgtttttga agagtgcgcg ccgctctttg gattctaaat
    53461 gtaaaattga ttccgctccc ggtcagcatg gtgcaaaaaa accgcgtttg tcagactatg
    53521 gtttgcagtt gcgtgaaaaa caaaaaatcc gccgtattta tggcgtatta gaacgtcagt
    53581 tccgtcgtta tttcgcagaa gctgatcgtc gtaaaggttc taccggcgag ttgctgttgc
    53641 agttgctgga atctcgtttg gataatgtcg tttatcgtat gggtttcggt tctacccgag
    53701 ctgaagcaag acagcttgtt tctcataagg cgatagttgt gaatggacaa gttgtcaata
    53761 ttccttcttt ccaagtgaaa gctggtgatg ttgtctcagt tcgtgaaaaa gccaaaaaac
    53821 aggtacgtat tcaagaagca ttgggtttgg caactcaaat cggcttgccg ggttgggttt
    53881 ctgtagatgc ggataaactt gagggtgtgt tcaaaaacat gccggatcgc tcggaattga
    53941 ccggtgatat taatgaacag ctggtggtag agttctactc taaataatgc tagctcagtg
    54001 agggacagtt aaatgcagaa tagcacaacc gaatttttga aacctcgtca aattgatgta
    54061 aatacttttt ctgcaactcg tgcaaaagta tctatgcagc catttgaacg tggtttcggt
    54121 cataccttag gtaatgcttt gcgccgtatc ttactgtcat ccatgaatgg ttttgctcct
    54181 actgaagtag ctattgccgg tgtattacac gaatattcta ctgttgatgg tattcaggaa
    54241 gatgttgttg acattttgct gaatattaaa ggtattgtgt ttaaactcca tggtcgtagc
    54301 caagttcaac ttgtgttgaa gaaatcaggt tcaggtgtcg tatctgccgg tgatattgag
    54361 ttgccgcatg atgtagaaat tctgaatcct ggtcatgtca tttgtcattt ggctgataac
    54421 ggtcaaattg agatggaaat taaagtagag caaggtcgtg gttatcaatc tgtttcaggt
    54481 cgtcaggtag ttcgtgatga gaaccgtcag attggtgcaa tccagttgga tgcgagcttt
    54541 tcgcccatca gccgtgttag ctttgaggtt gaacctgcac gtgtagagca gcggacggat
    54601 cttgataagt tggttttgga tatcgaaacc gacggttcta ttgatcctga ggaagctgta
    54661 cgcagtgcgg cacgtatttt gattgatcag atgtctattt ttgctgattt gcagggtacg
    54721 cctgtggagg aggttgaaga aaaagcacct cctatcgacc ctgttctttt gcgtccggtg
    54781 gatgatctgg aattgacagt acgttcagct aattgtttga aagctgagga tatttattat
    54841 attggcgatt tgattcaacg cactgaaacc gagcttctta aaacgccgaa tttgggacgt
    54901 aaatctttga atgagattaa ggaagtattg gcatctaaag gtttgacact gggttctaag
    54961 ttggaagcat ggccacctgt aggcttggaa aagccttaat gaagaattaa aggataattg
    55021 atatgcgtca tcgtaatggc aatcgcaaat taaaccgtac cagcagtcat cgtgctgcaa
    55081 tgctgcgtaa tatggcgaat tcattattga ctcacgaaac tattgtaaca actctgccta
    55141 aggccaagga attgcgccgt gtagtagagc cgttgattac attgggtaaa aagccgtcat
    55201 tggcaaaccg ccgtttggca tttgaccgta ctcgcgaccg tgatgttgta gtaaaactgt
    55261 ttggcgattt gggtcctcgt tttactgctc gtaacggtgg ttatgttcgg gtgttgaaat
    55321 acggattccg taaaggtgat aatgcacctc tggcactggt tgaattggtt gacaaaccgg
    55381 ctgctgagta attttagtca tataacgcca tctgccgaaa agcaggtggc gttatttttg
    55441 caatatctga taggtaatag ggtattggct atcatgttta aaatattaat tgaatagcta
    55501 aggtttgcgc ggtaaactta catcattaaa aaattctatg atggtttata taatgaatgc
    55561 cttcgatata aagtcgacaa agatggacgt attgtctata tctttgcata cgtcagactt
    55621 gtttgatttg gaagatgtgc tggtcaaatt gggcaagaag tttcaagagt ctggtgttgt
    55681 tccatttgtg ctggatgttc aagagtttga ttatcccgag tctttggatc ttgctgcatt
    55741 ggtttcgttg ttttcaaggc atggtatgca aattttgggt ctgaagcatt ctaatgaacg
    55801 ttgggctgct gcggctatga agtatcattt gctgttttgt ctgtctcatt cggaaaatgt
    55861 taaagaactg ggtcaggttg aggtgcagaa cacggaggat ggtcagaaag caaggaaaac
    55921 agtattgatt acatcccctg tccgtaccgg tcagcaggtt tatgccgaag atggcgattt
    55981 gattgttacg ggggcggtca gccagggggc ggaattgatt gcggatggca atatacatat
    56041 ttatgcgccg atgagggggc gtgctttggc cggtgccaag ggtgatactt ctgcccgcat
    56101 atttatccac tccatgcagg cagaactggt ttctgtggcg ggtatttacc gtaattttga
    56161 acaggatttg ccgaaccatc tgcacaagca gccggtacag atattgttgc aggataaccg
    56221 attggttatc agtgcaattg gctcagagta attgtttgat atttaaaaag gaaatattgt
    56281 ggcaaaaatt attgtagtaa cttcaggtaa gggcggtgtc ggtaaaacga ctaccagtgc
    56341 cagtattgcg acaggtttgg cattacgcgg atataaaact gcggtaattg attttgatgt
    56401 gggtttgcgt aacctcgacc tcattatggg ttgcgagcgt cgtgtcgttt atgacctgat
    56461 caatgtcatt cagggggagg cgacgctcaa ccaagctttg attaaagata aaaattgtga
    56521 aaacctgttt attttgccgg cttcccagac tcgggataaa gacgctttga cacgcgaggg
    56581 cgtagaaaaa gtgatgcagg agctgtccgg caagaaaatg ggctttgagt atattatttg
    56641 cgactctcct gccggtattg agcagggtgc attgatggcg ttgtattttg ctgatgaagc
    56701 cattgtaacg accaatcctg aggtttccag tgtgcgtgac tccgacagga ttttgggaat
    56761 tttgcaaagc aaatcccata aggcagagca aggcggttcg gttaaagaac atctgttgat
    56821 tacgcgttat tctcccgaac gtgtggcaaa aggcgaaatg ctgtctgtac aggatatttg
    56881 cgatattctg catattcctt tgctgggtgt gattcctgaa tcccaaaacg tcttgcaggc
    56941 atccaattcc ggagaaccgg tcatccatca ggacagcgtg gcggcttccg aggcatataa
    57001 ggacgttatt gcccgtcttt tgggcgagaa ccgtgaaatg cgtttcttgg aagctgagaa
    57061 aaaaagcttc ttcaaacgtc tgtttggagg ataaggtatg tcattaatcg aatttttatt
    57121 cggcagaaag cagaaaacgg caaccgttgc ccgcgaccgc cttcaaatca tcattgccca
    57181 agagcgcgcc caagaaggtc aggctccgga ttacctgccg actttacgta aagagttgat
    57241 ggaagtcctg tccaaatatg tgaatgtttc attagacaat atccgtattt cccaggaaaa
    57301 acaggatggt atggatgtgc ttgagttgaa cattactttg ccggaacaga aaaaggtata
    57361 ggacatgacc ttaaccgaat tgcggtacat cgtcgcagtc gcccaagaac gtcatttcgg
    57421 cagggcggcg cggcgttgtt ttgtcagcca gcccactttg tctattgcca ttaagaaatt
    57481 ggaagaagag cttgccgtct ctttgtttga ccggagcagc aacgatatta ttacgaccga
    57541 ggcgggggaa cgtatcgttg cacaggcgcg taaggtgttg gaagaggcag agcttatcag
    57601 gcatttggca aatgaagaac aaaacgagct ggagggtgcg ttcaaactcg ggctgatttt
    57661 tacggttgcg ccgtacctgc tgccgaaact gattgtctcg ttgcgccgta ctgcaccgaa
    57721 aatgcctttg atgttggaag agaattacac gcatactttg accgagtcgc tcaaacgcgg
    57781 ggacgttgac gcgattatcg ttgccgaacc gtttcaagag ccgggcattg ttaccgaacc
    57841 cttgtatgac gaaccgtttt tcgtgattgt cccgaaaggg cattcatttg aggaactgga
    57901 tgccgtttcg ccccagatgc tgggtgagga gcaggttttg ctgctgacgg aaggcaactg
    57961 tatgcgggat caggtactct caagctgttc cgaattggcg gcgaaacaac gcatacaggg
    58021 gttgaccaat acattgcagg gcagctcgat caatacaatc cgccatatgg tcgccagcgg
    58081 tttggcaatc agcgtgttac cggcaaccgc gctgaccgaa aacgatcata tgctgttcag
    58141 cattattccg tttgaaggta cgccgccaag ccggcgggtc gtattggcgt accgccgcaa
    58201 ttttgtccgt ccgaaggcgt tgtcggcgat gaaggcggcg attatgcagt cgcagcttca
    58261 cggggtaagt tttatccgcg actaggcgca ggcattgttt tcaaaacgcc atttccctga
    58321 gccgacaaca cggtatgcca agatattgcc gtcatcatcg attttgagta tagcatcgcc
    58381 acggaaactg ccgtcctgaa gatattcgac ttttgcatca ctgtgaatgt tttcatcagt
    58441 gccgatgcaa tgccatgtat agtggattaa caaaaaccag tacggcgttg cctcaccttg
    58501 ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta
    58561 taaaagaggc cg
//
LOCUS       DAXHSJ010000002        31914 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000002 DAXHSJ010000000
VERSION     DAXHSJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31914
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.002"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..176)
                     /locus_tag="ACFM8U_000078"
     CDS             complement(<1..176)
                     /locus_tag="ACFM8U_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6333396.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCKPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIP"
     gene            complement(564..839)
                     /locus_tag="ACFM8U_000079"
     CDS             complement(564..839)
                     /locus_tag="ACFM8U_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6333397.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(836..2119)
                     /locus_tag="ACFM8U_000080"
     CDS             complement(836..2119)
                     /locus_tag="ACFM8U_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6333398.1"
                     /translation="MTVSKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWTEVFVITSLFMLPGLLMTLFLAREPVLPLAVSKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGMWMLKIGVNKALWLFGAVQAITVLGFVWLAGFGHFDTVGTSERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFRLCFILALPGMLLLLKVAPWNGEKTRDAGR"
     gene            complement(2269..3687)
                     /gene="glnA"
                     /locus_tag="ACFM8U_000081"
     CDS             complement(2269..3687)
                     /gene="glnA"
                     /locus_tag="ACFM8U_000081"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC6333399.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3712..3921
                     /locus_tag="ACFM8U_000082"
     CDS             3712..3921
                     /locus_tag="ACFM8U_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333400.1"
                     /translation="MAKYAERGGLTQSRHDFTTPMPSETGFASNFKPENIVSDGKIVN
                     NMLRFVCSKAECPPFCPNADKTDAV"
     gene            3999..4808
                     /gene="aroE"
                     /locus_tag="ACFM8U_000083"
     CDS             3999..4808
                     /gene="aroE"
                     /locus_tag="ACFM8U_000083"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC6333401.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHRQFALQEGVEIEYGRICADIG
                     SFAQAVLTFFGTGGCGANVTVPFKQEAFDLADEHSERASAAGAVNTLILLKNGKLRGD
                     NTDGLGLVGDIVKVQHTEIEGKNILLLGAGGAVRGVIPVLLAQNPARIVIANRTRAKA
                     EEVAARFGIEAVSPDSLAGGFDIIVNGTSGGLSGQIPDIPPDIFQNCALAYDMVYGEA
                     AKPFLDFARQSGAKQTADGLGMLVGQAAASYALWRGFKPDIRPVIEYMKAL"
     gene            4810..5511
                     /gene="mtgA"
                     /locus_tag="ACFM8U_000084"
     CDS             4810..5511
                     /gene="mtgA"
                     /locus_tag="ACFM8U_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC6333402.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVPLDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGEVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLSKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LKRMGSAELPEDDTD"
     gene            complement(5713..5788)
                     /locus_tag="ACFM8U_000085"
     tRNA            complement(5713..5788)
                     /locus_tag="ACFM8U_000085"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5753..5755),aa:Phe,seq:gaa)
     gene            complement(5979..6713)
                     /gene="lptB"
                     /locus_tag="ACFM8U_000086"
     CDS             complement(5979..6713)
                     /gene="lptB"
                     /locus_tag="ACFM8U_000086"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC6333403.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGENFKY"
     gene            complement(6787..7317)
                     /gene="lptA"
                     /locus_tag="ACFM8U_000087"
     CDS             complement(6787..7317)
                     /gene="lptA"
                     /locus_tag="ACFM8U_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC6333404.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQGDSRQPIQIEADQGSLNQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRNDKGEQFMKADGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVIIQPSSTQKSE"
     gene            complement(7298..7879)
                     /gene="lptC"
                     /locus_tag="ACFM8U_000088"
     CDS             complement(7298..7879)
                     /gene="lptC"
                     /locus_tag="ACFM8U_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC6333405.1"
                     /translation="MKIRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVKTEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGLLNFSSKVKATIYDTKDM"
     gene            complement(7876..8412)
                     /locus_tag="ACFM8U_000089"
     CDS             complement(7876..8412)
                     /locus_tag="ACFM8U_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC6333406.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPCGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(8585..9559)
                     /locus_tag="ACFM8U_000090"
     CDS             complement(8585..9559)
                     /locus_tag="ACFM8U_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFC6333407.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMYDLLAARIV"
     gene            9655..10710
                     /gene="tal"
                     /locus_tag="ACFM8U_000091"
     CDS             9655..10710
                     /gene="tal"
                     /locus_tag="ACFM8U_000091"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC6333408.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALEILVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKTAYQDWEQYFTAPKFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVDEARARLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            10852..11142
                     /locus_tag="ACFM8U_000092"
     CDS             10852..11142
                     /locus_tag="ACFM8U_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333409.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLIGRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALIHSLK"
     gene            11159..12046
                     /gene="gluQRS"
                     /locus_tag="ACFM8U_000093"
     CDS             11159..12046
                     /gene="gluQRS"
                     /locus_tag="ACFM8U_000093"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC6333410.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSHRYALYEETLCRLQTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPAPQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITTP"
     gene            complement(12114..13124)
                     /gene="dusA"
                     /locus_tag="ACFM8U_000094"
     CDS             complement(12114..13124)
                     /gene="dusA"
                     /locus_tag="ACFM8U_000094"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC6333411.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYLARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDAMLLKDNDGGLILEAWEEVERANT
                     RE"
     gene            complement(13155..13620)
                     /gene="dtd"
                     /locus_tag="ACFM8U_000095"
                     /pseudo
     CDS             complement(13155..13620)
                     /gene="dtd"
                     /locus_tag="ACFM8U_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
     gene            complement(13694..14452)
                     /locus_tag="ACFM8U_000096"
     CDS             complement(13694..14452)
                     /locus_tag="ACFM8U_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6333412.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQEQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(14523..15389)
                     /locus_tag="ACFM8U_000097"
     CDS             complement(14523..15389)
                     /locus_tag="ACFM8U_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6333413.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(15438..15716)
                     /locus_tag="ACFM8U_000098"
     CDS             complement(15438..15716)
                     /locus_tag="ACFM8U_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6333414.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(15716..16006)
                     /locus_tag="ACFM8U_000099"
     CDS             complement(15716..16006)
                     /locus_tag="ACFM8U_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC6333415.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(16009..16539)
                     /locus_tag="ACFM8U_000100"
     CDS             complement(16009..16539)
                     /locus_tag="ACFM8U_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC6333416.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            16847..19522
                     /gene="tspA"
                     /locus_tag="ACFM8U_000101"
     CDS             16847..19522
                     /gene="tspA"
                     /locus_tag="ACFM8U_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
                     /protein_id="HFC6333417.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETV
                     AETPDFNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENVTKTVA
                     EIPDFNATADDLSALLQPSEVPAVEENVTETVAETSDFHTAADDLSALLQPFETPAVE
                     KFEAPAIEEKAAETVDKTPDFHTVADDLSALLQPAEVPAVEENAAEITLETPDSNTSE
                     ADALPDFLKDGEEETVDWSIYLSEENIPNNADTGFPSESVGSDAPSEAKYDLAEMYLE
                     IGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(19695..20111)
                     /gene="gloA"
                     /locus_tag="ACFM8U_000102"
     CDS             complement(19695..20111)
                     /gene="gloA"
                     /locus_tag="ACFM8U_000102"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC6333418.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(20135..21304)
                     /gene="lapB"
                     /locus_tag="ACFM8U_000103"
     CDS             complement(20135..21304)
                     /gene="lapB"
                     /locus_tag="ACFM8U_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC6333419.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(21315..21629)
                     /locus_tag="ACFM8U_000104"
     CDS             complement(21315..21629)
                     /locus_tag="ACFM8U_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC6333420.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            21939..22937
                     /gene="ilvE"
                     /locus_tag="ACFM8U_000105"
     CDS             21939..22937
                     /gene="ilvE"
                     /locus_tag="ACFM8U_000105"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC6333421.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(23181..23966)
                     /gene="fabI"
                     /locus_tag="ACFM8U_000106"
     CDS             complement(23181..23966)
                     /gene="fabI"
                     /locus_tag="ACFM8U_000106"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC6333422.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(24121..24942)
                     /gene="dapD"
                     /locus_tag="ACFM8U_000107"
     CDS             complement(24121..24942)
                     /gene="dapD"
                     /locus_tag="ACFM8U_000107"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC6333423.1"
                     /translation="MSLQNIIETAFENRADINPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEILNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPTGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(25109..26752)
                     /gene="pgi"
                     /locus_tag="ACFM8U_000108"
     CDS             complement(25109..26752)
                     /gene="pgi"
                     /locus_tag="ACFM8U_000108"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6333424.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYVDGRDVLPEIRRELNRALEFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETGCHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPVTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            27109..28341
                     /gene="pilG"
                     /locus_tag="ACFM8U_000109"
     CDS             27109..28341
                     /gene="pilG"
                     /locus_tag="ACFM8U_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC6333425.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            28415..29275
                     /locus_tag="ACFM8U_000110"
     CDS             28415..29275
                     /locus_tag="ACFM8U_000110"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC6333426.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            29277..29909
                     /gene="coaE"
                     /locus_tag="ACFM8U_000111"
     CDS             29277..29909
                     /gene="coaE"
                     /locus_tag="ACFM8U_000111"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC6333427.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            29902..30111
                     /locus_tag="ACFM8U_000112"
     CDS             29902..30111
                     /locus_tag="ACFM8U_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC6333428.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(30196..31872)
                     /gene="pilB"
                     /locus_tag="ACFM8U_000113"
     CDS             complement(30196..31872)
                     /gene="pilB"
                     /locus_tag="ACFM8U_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC6333429.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         7492 a         8978 c         8175 g         7269 t
ORIGIN      
        1 gggataacca ttttggtgcc ctgaaaatca aatgtaaccg tatgttcaaa tcctgtcatt
       61 ggctcgggat tgttgaaact ggtttgttca ttaacaggtt tacatgaggt catagtaaaa
      121 acactcccaa taaacaaaat aaaaagttga taaatagtag tagcatatgg tctcataatt
      181 tcaagcctag aaattagtta aagaatatat tcatggtaga tactaactct ttaagttgac
      241 atattgtgcg atatcgaggg ctgtaccata ttttcccatc gggtcttccc cgttctttat
      301 atgagcggca ggcatcctca taaaagaagg gataccgata aggaaaaata cctcggcata
      361 aaaaatatac tgattaatca atacaaatcc aacaaacagt ttatcatttt cttttctgtt
      421 ggcgtataaa aaatactgat atatgccttc actttcatag ggcgacgctt tatgtaccgt
      481 cctgtgtgtt gaaacatcgg ttttccttac cgcctgccct gcttttccct gattgctgcc
      541 aactgccgtc cgaacttgaa ccgtcaggtt tcagacggca ttttttctgc ctgttcccac
      601 aatttccgtt tttgtgcggc ggtcagtttt ttgacggcga tttcttgttt gagcgcggtg
      661 cctttatcca tcccgcctgc aacgatacgc atcgctaccg gtttgaatac gcgggtatat
      721 ttcgcgcctt ttccggctgt gtgggcggca agccgctgtt gcggattcgg gctgatgccg
      781 caatagaacg cgctgttttc acacaatatc aggtaaacgc tccagtttga cgcgttcatc
      841 tgcctgcatc ccgagttttc tccccgttcc aaggcgcaac tttcagcaac aacagcatac
      901 ccggcagggc aagtatgaaa cacagccgga aaaacggtac ataaccgagc cattcgatca
      961 gataaccggc aaaggaattg atgaccgtac ggggaacagc ggacagactg gtaaacagcg
     1021 cgagctgcgt tgctgtaaac gcgggattgg tctcgcgcgc catatacgat acgaacgccg
     1081 ccgtccccaa ccccacgccg actgcctctg cgccgataac tgccgccagc atcagcctct
     1141 cgcttgtacc gactgtgtcg aaatgtccga acccggccag ccatacaaac cccaaaaccg
     1201 ttattgcctg caccgcgccg aatagccaca aggctttgtt cacgccgatt ttcagcatcc
     1261 acatgccgcc caagatacct gccgccactg ccggccacag tcctgcattt ttggcaatca
     1321 aaccgatgtc ggtcttgctg aaacccatat ccagataaaa cggcgttgcc aacgcggttg
     1381 ccatactgtc gccgagtttg taaaggaaga taaacagcag cacgcataca gccgaagcga
     1441 tgcccttgcg tgtaaaaaat tctttaaacg gctctaccac ggtctgtttc aacgttttag
     1501 aaacagcaag cggcaacacg ggttcgcgcg cgagaaatag cgtcatcaga agaccgggca
     1561 gcataaacag cgaagtgatg acaaacacct ccgtccacgg catcctgtct gccaacacca
     1621 aactcaatga accgggaatc agggcggcaa cccgataggc gttcacatga accgagttgc
     1681 ccaaacccaa ttcttcgtcc gacaaaatct cgcgcctgaa cgcatccaat acaatatcct
     1741 gactggcgga aaaaaaagca acaagcaccg acaagccagc aatcaacggc agatgattac
     1801 gggggtttaa aaaggcatat gccgccaaag ccgccagcaa ccctgcctgc gtcaacagca
     1861 tccacccgcg ccgccgcccc aaaacgggca gcctgaccgc gtccatcagc ggggaccaca
     1921 aaaatttcca agtaaacggc agaccgatta acgccatcag cccgatgctc ttcaaatcca
     1981 cctgctcgct gcgtaaccat gccggaatca ggttaatcag aaagtacagc ggcagccccg
     2041 aggtaaaccc cgtaaaaata cagacaagca tatttcggga aaagatttgc ccgataaaac
     2101 ctgatttcga tacagtcatg gttcaaacag ccaagaaata aaagcctatt gtagcaaata
     2161 tcgcccaacc gctgaaaaac accgcccctg ctccgccgtt ttgccgttca aatgcaaaat
     2221 gccgtctgaa actttgttca gacggcattt ctactgaaac ctgagagttt acaggctgta
     2281 atacatttca aattccagcg gatgcggtgc catgcggatg cggcggacat cttcctcttt
     2341 aaaggcgatg tagctgtcga tccagtcttt gctgaacacg ccgccgcgca ggaggaattc
     2401 gtggtcggct ttgagggcgg ccagtgcttc ttccaaagag gcgcaaacgg tagggacgag
     2461 cgcgtcttct tccggcggca ggtcgtagag gtttttatcg gcagggtcgc ccggatggat
     2521 tttgttttga ataccgtcca aaccggccat caacaaagcg gcgaacgcca agtatgggtt
     2581 ggcggtcggg tcggggaaac gcgcttcgat gcggcgcgct ttgctgctgt tcacagacgg
     2641 aatacggatg gacgcggaac ggtttttggc ggagtaggcc agtttggtcg gcgcttcgaa
     2701 gtgcggcacg aggcgtttgt aggagttggt ggacggattg gtaatcgcgt tcagggcttt
     2761 ggcgtgtttg atgatgccgc cgatgtagta gagcgcggtg tcgctcaagc cggcatagcc
     2821 gtcgcctgcg aacaggtttt gaccgtcttt ccaaatggat tggtgaacgt gcataccgct
     2881 gccgttgtcg cccataatcg gtttgggcat aaaggtggct gttttgccga agttgtgggc
     2941 aacgttttga atcacatatt tcatgtcttg ggtttggtcg gcgcgtttga ccaaggtggc
     3001 gaaacgcgtg ccgatttcca tttggctgcc ggtaccgact tcactgtggt ggacttcgac
     3061 ttcgatgccg agttcttcca aaatgtttac catcgcggag cgcaggtctt ggccgcaatc
     3121 aatcggcgcg acgggggcgt aaccgccttt gacggcaggg cggtggccgg tgttttgacc
     3181 gtccatgtgc agaccgcttg cccatgcgcc gctttcggac gtgatttcgt aacgggtttt
     3241 gtgcatgtcg gtttcaaatt ctacgccgtc gaagacgaag aattcaggct cggggccgaa
     3301 gtatgccgtg tcgccgatgc cggaagattt caaataggcc tcggcgcggc gtgcgatgga
     3361 gcgcgggtcg cggtcgtaac cctgaccgtc ggcagggtcg atgacgtcgc aggtaatgac
     3421 gacggtaaca tcatcataaa aaggatcgac gaaggctgta gacgcatcgg gacgcagctg
     3481 catatcggaa gcctgaatgc ctttccagcc gccgatagac gaaccgtcaa acgcctgacc
     3541 gttttcgaac cactcttcgg ggtcttccaa cacgatgcgc gcaggcacgg taaagtggtg
     3601 ctgcttgcct ttggtatcgg taaagcgcaa atcgacaaag cgggcttcgc tttcttcaat
     3661 caattttacg gcgtttttaa tggacatttt tcagctcctg gaaaaaggga aatggcaaaa
     3721 tatgcggaac gcggcggctt gacgcaaagt cggcacgatt ttaccacgcc gatgccgtct
     3781 gaaaccggtt ttgcgtcaaa tttcaagccg gaaaatatag tttcagacgg caagattgtc
     3841 aacaatatgc ttcggtttgt ctgttccaaa gcggaatgcc cgcccttttg tccgaatgcc
     3901 gacaaaacag atgccgtctg aaagcagttc agacggcatc gttcccatct tcgcgccgcc
     3961 gttataaaat gaccgccccg ccttgatgga atatgactat gcacgccctc ccccgctacg
     4021 ccgttttcgg caaccccgtt gcacacagta aatcgccgca aatccatcgg cagtttgccc
     4081 ttcaggaagg cgttgaaatc gaatacggac gcatttgcgc cgacatcggc agttttgcac
     4141 aggcggtttt gacatttttt ggaacaggcg gttgcggggc aaacgtaacc gtcccgttca
     4201 agcaggaagc gtttgacttg gcggacgaac attccgaacg cgcatcggcg gcaggcgcgg
     4261 tcaatacgct gattttgctg aaaaacggaa aactgcgcgg cgacaatacc gacggtttgg
     4321 gcttggtcgg cgatatagtg aaggtgcaac acacagaaat tgaaggcaaa aatatcctat
     4381 tgctcggcgc gggcggcgcg gtgcgcggcg tgattcccgt attgctggcg caaaatcccg
     4441 cccgcatcgt catcgccaac cggacgcgtg ccaaagcgga agaagttgca gcgcgtttcg
     4501 gcatcgaagc cgtctctcct gacagtttgg caggcggttt cgacatcatc gtcaacggca
     4561 catccggcgg tttgagcggt cagattcccg atattccgcc cgatattttt caaaactgcg
     4621 cgcttgccta cgatatggtg tacggcgaag cggcaaaacc gtttttggat tttgcccggc
     4681 aatcgggcgc gaaacaaacc gccgacggac tgggtatgct ggtcgggcag gcggcggctt
     4741 cctacgccct ctggcgcggt ttcaaaccgg acatccgccc cgtcatcgaa tatatgaaag
     4801 ccctgtaata tgttccgcat cgtcaaatgg ctgattgccc tgcccgtcgg catctttatc
     4861 tttttcaatg cctatgtgta cggcaacatc atcacctacc gcgccgtcgc gccgcatcgg
     4921 actgccttta tgtcgatgcg gatgaagcag tttgaacaag aagggcgcga tgttcctctg
     4981 gattaccgct ggatgcccta caaccgcatt tcggtcaacc tgaaaaaagc cctgattgct
     5041 tccgaagatg cccgtttcgc cggacacggc ggcttcgatt ggggcggcat tcaaaacgcc
     5101 atcaggcgca accggaacag cggcgaagtg aaggcgggcg gatcgaccat cagccagcag
     5161 cttgccaaaa acctgttttt gaacgaaagc cgcagctata tccgcaaagg cgaagaagcc
     5221 gcgattactg cgatgatgga agccgttacc gacaaagaca ggatttttga actgtattta
     5281 aactcaatcg aatggcacta cggcgtgttt ggtgcggaag ccgcgtcccg gtatttttat
     5341 caaatacccg ccgccaaact gagcaaacag caggcggcga aactgactgc ccgcgtcccc
     5401 gccccgctct actacgccga ccatccgaaa agcaaacggc ttcgcaacaa aaccaatatc
     5461 gtgctcaaac gtatgggttc ggcagaattg cctgaagacg acacggactg agtgccgccg
     5521 cgatataaaa tgccgtctga aatatcattc agacggcata tgctttctgc ctttaccctc
     5581 ttctccaaaa cgcaacgggg cggtcattat catttttgcc cttctacata acggtcagac
     5641 acaaaacgtg tccgacacgg acaatttccc aaaaacggca ggcaaaaaaa taaccgcttc
     5701 aaagcggttt tttggtggcc agaggcggaa tcgaaccgcc gacacacgga ttttcaatcc
     5761 gttgctctac caactgagct atctggcctt tgtctgtctc attaagagat gcgtattaaa
     5821 ccaaattacg ggacaacacg caagcggatt tgtcgatatt ttttacccaa ccgactaagt
     5881 cgatgtttta agtttaaatt aatttcacag aaaaatagtg caaatcggtt ttttgccgtt
     5941 tgttcggcga tgttctgctt ttcagacggc aaaacgcttc aatatttgaa gttctcaccc
     6001 agataaacag aacgaacctg ttcgtttccg accaaatcat caggttttcc cgatgccagc
     6061 accgtgccgt ctgaaataat gtaggcgcgg tcgcagatgc tgagggtttc gcgtacgttg
     6121 tggtcggtaa tcagtacgcc gatgccgcgc gatttgagaa atccgatgat tttttggatg
     6181 tcgatgacgg cgatgggatc gacgccggca aaaggctcgt ccaacaaaat aaaatgcggt
     6241 ttcatggcaa gtacgcgggc gatttcgacg cgtcggcgtt cgccgcccga caaagatggg
     6301 gcaggattat tgcgcaaacg ctcgatattc aggtcggcaa gcagtttttc aacttcgccg
     6361 tcgatgcggc ttttgtcttt ggtactgatt tctaaaatgg cgcggatgtt ttgttcgacg
     6421 gtcattttac ggaaaataga ggcttcctgc ggtaggtagc cgacacccag gcgggcgcgt
     6481 tcgtgtatgg gcaggtgacg caattcctgc ccgtccagca tcacgctgcc cgcatcggcg
     6541 gcaatgagtc caacaatcat gtagaagctg gtggttttac ccgcaccgtt gggcccgagc
     6601 aggccgacga cttccccgct ttcgatttca agggagaagc ttttaacgac ttggcgtttt
     6661 ttaaaactct tttgcaggtt ttgaacaaca aggcggctga cgtttgcgct cataaatatc
     6721 tcttcaatat cttcggtcgg catatgccgt cggaaccggg tttatgtttc agacggcatt
     6781 ttgggattat tcggattttt gcgtactcga aggctggatg atgacgctga ccctgccgga
     6841 tttggaagcg gattttgcgc cggattttgt actgccgctg atggtataga cttcggtttt
     6901 ggtgttgtag gtaatcaccg cgccttcggc gacatcgccg ccgcgctgta ctttggcatt
     6961 accggttaag actacggtgc tgcctgcaga cgaataagca acgttgtttg cctgaccgcg
     7021 caccgtccct ttgcccccgt ccaacgtttg gctgaagcgt acgggcgagc cgtcggcctt
     7081 cataaactgt tcgcctttgt cattacgcgt tacgttgacg cgggcggcgg aaatattgag
     7141 cgtaccctgt ctgatgacaa cgtttccgct aaatgtagtg ctttggttgg cttgattgag
     7201 agaaccttgg tcggcctcaa tctgaatagg ctgcctgctg tcgccttgaa gggcaaaagc
     7261 gggagatgcc gaaaaaaatg ctattaaaac aaatagctta catatctttt gtatcataaa
     7321 tcgtggcttt cactttagat gagaagttca acaggcctgt tttgtggtcg taagtcatgc
     7381 cgcccgcctg cccgtgcgac gcaccgtatt gaaagctgac gggcgtatcg gtttgggcat
     7441 attgagattc ggtatcgacg tgcagctttt cggttttaac tttacccgcc tgccgtttgc
     7501 catcggcggt tttggtcagc acaacgttgt ttttaaaaag aacctgtttg ttttcggtat
     7561 ggtaaacggc ttcatcgctg ccgacttcgt acaacaacct gccttcttgg aagaacacga
     7621 gatgcggcga atcaaaatgg atgtcgctgc tttcgggaaa ctgtttcgcg cctttcgcgc
     7681 tcaaatgttc ttttaagtat ccctgttcgt caaaccgcct gccatccaat ccgtccattg
     7741 tgtattgcgg ttcgtcggga ttgagcctga cttcttcaat ctcgacttcg ctgatgcgcc
     7801 ccaaccatgc cgacaggctg cccaaggcaa ccgccaatat caatgggaac gcaattccgt
     7861 accgccatct tattttcatt tgatgtactc gttcaaagcc gcgcccaaag tcccttgcgc
     7921 ctgcataatc aggtcgcaca cttcgcgcac cgcgcccgca ccgcaggggc gtttggcaat
     7981 ataggcggcg tgttgcagcg taaaccaatg cgcgtcgggg acggcaaccg gcaatccgca
     8041 acgcaccatc accggcaaat cgaccacgtc gtcgccgaca aaggcacact cggcttcttc
     8101 cacgccaacc tgagcgcgca gctcttcata cgcggcgcgt ttgtccgaaa tgcctttgaa
     8161 gtagtaattt atgcccaact gtttgacgcg gatgccgacg gagggcgcgt cccgacctgt
     8221 gataatcgca gtctgcacgc cgcttgcctg aagcattttc agaccgtgtc cgtccagtgt
     8281 gtgaaacgat ttgatttctt cgccgttatc gcggataaag atgcgcccgt cggtcaaaac
     8341 gccgtccaca tccaatatca gcagtttgat tttggcggcg cgcgcctgta attcgggaga
     8401 aattgcctgc atgggatttc cttgttttca gacggcacat tttagcacaa atgccgtctg
     8461 aaacggctgc aacgccttat atagtggatt aacaaaaatc aggacaaggc gacgaagccg
     8521 cagacagtac agatagtacg gcaaggcaag gcgaggcaac gctgtactgg tttttgttaa
     8581 tccactatac aatccgtgcc gccagcaggt cgtacatatt cagcgcgcca atcagtacgc
     8641 cgtcggcatc agtaaccaga agcccattca catggtttgc ctgcatgact ttcagggctt
     8701 cggtggcgag gcgttcggcg gagatggttt taggatgcgt atgcatgact tcgtctatcg
     8761 aaagaccggt aaaattgtcg cattcttgaa acaggcggcg caaatcgccg tcggtgaata
     8821 cgcctttcag acggccttgc ccgtccgtta ccgccagcat ccccagccct ttttcactca
     8881 tgctgacgat ggcttctttc aagggcgtgc cgagtcggac ggcgggcagg ccgccgcctt
     8941 tgtgcataat gtcggcaacg cgcaaaagta ggcgtttgcc gaggctgccg gcaggatggc
     9001 tcaaggcgaa atcgtcgggc gtgaacgcgc gtgcgcgcag caggacgacc gccaacgcat
     9061 cgcccaaagc catgacggcg gtggtgctgg tggtcggggc aagccccagc gggcaggctt
     9121 ctttggaaac cgacgccgtg atgtggatgt cggcatggcg cgccatggtt gaatcggggc
     9181 gggcggtgat gcagacaagc gtgatgtctt tgcgtttgag cgcggggatg atggcggcga
     9241 tttcgtcgct ttcgccggaa ttggaaatcg cgacgaccac gtcgttgtcc acaatcatgc
     9301 ccaaatcgcc gtgtgccgct tccgcagggt ggacgaaaaa cgcgggcgtg ccggtcgagg
     9361 ccatggttgc cgccattttg cgcccgatat gtcccgactt gcccatgccc gtgataacaa
     9421 ccctgccctt gcagtgcaac aacgcgtctg ccgcaaggac gaagttttcg tccaattccg
     9481 ctgcaatttc gcgcaagcct tccgcttcgg tgtgcaacac ttcgcgcgcc cagtcgagat
     9541 attttccgtt ttctgccatt gccatccgcc tttgctgtaa caaaactacc caaaatttcc
     9601 gaatcggggt agcataagca ttcaaacccc gccatccatc caaaggaatc agaaatgact
     9661 attttatcgg acgttaaagc attaggacaa caaatctggc tggacaacct ttcccgctcg
     9721 ctcgtgcaaa gcggcgaatt ggcgcaaatg ctgaaacaag gcgtgtgcgg cgtaacttcc
     9781 aatcccgcca tcttccaaaa agccttcgcc ggcgacgcgc tttacgccga tgaaatcgcc
     9841 gccctcaagc agcaaaacct cagccccaaa caacgctacg aaaccatggc ggttgccgat
     9901 gtgcgtgccg cctgcggcgt ttgccttgcc gaacacgaat ccaccggcgg caaaaccggt
     9961 ttcgtcagcc tcgaagtttc gcccgaattg tccaaagacg cgcaaggcac ggtagaagaa
    10021 gcgcgccgcc tctacgccgc catcgggtgc aaaaacgcga tgattaaagt gcctgccacc
    10081 gacgcaggca tcgatgcgct cgaaatcctc gtttcagacg gcatcagcgt aaacctcacg
    10141 ctgctgttct cacgcgccca aaccctcaaa gcctacgccg cctacgcgcg cggcattgcc
    10201 aaacgcttgg cagccggaca aagcgttgcc catatccacg tcgtcgccag cttcttcatc
    10261 tcccgtgtgg acggcgcgct ggacacaacg ctgcccgacc acctcaaagg caaaatcgcc
    10321 atcgcccttg ccaaaaccgc ctatcaagat tgggaacaat atttcaccgc ccccaaattt
    10381 gccgcactgg aagcccaagg cgcaaaccgc gtgcagcttt tatgggcatc taccggcgtg
    10441 aaaaacccag cctatcccga cacgctctac gttgacagcc tgatcggcgc gcacaccgtc
    10501 aacaccgttc ccgatgccac gctcaaagcc tttatcgacc acggcacagc caaagccacg
    10561 ctgaccgaag gcgtggacga agcacgggcg cggctcgctg aaattgccgc gctcggcatc
    10621 gatgtcgaaa ccttggcggc gcgtttgcag gaagacggtt tgaaacagtt tgaagaagcg
    10681 tttgaaaaac tgctcgcacc gttggtttaa acttttcatc cccgatgccg tctgaaaggc
    10741 tttcagacgg catcgccctt ttgaccggac acagtgggtt caccctaaaa atattaatca
    10801 acaagaagtt aatgcacgac attacccacc aacacaaaag gaaatagaat catggcaaaa
    10861 gcactcgaaa tcatttcacc caatgaaatt tattcagatc taatttttaa ggatccggta
    10921 cctccccata ctatatatac agaattgatg aaactgattg gtagaaatgc aagtaatgag
    10981 agaatcgcag atagagcctt cgattttttc tcgtccggcc ttatggatgg ttcagctacc
    11041 gaagagcaat ataatgctct atatgattta accttgcttg aagagcccgg gatggaactg
    11101 aataaagatg agataatggc tttgattcat agtcttaaat aaaggggctg taccggatat
    11161 gtacacagga cgtttcgccc ccagcccgac cgggctgctc cacatcggct cgctgctgac
    11221 cgccgccgct tcctatgccg atgcacgctc aaacggcggc aaatggctgg tccgcatgga
    11281 agacctcgat ccgccgcgcg aaatgccggg ggcggcaagc catatcctgc acacgcttga
    11341 ggcattcgga ttcgagtggg acggagaagt cgcctatcag agccaccgtt acgccctgta
    11401 tgaagaaacc ctatgccgtc tgcaaaccgc cggactggtc tatccctgcc attgcagccg
    11461 caaagactgg caggccgggg caaggcgggg cgcagacgga ttcgtctata acggacgttg
    11521 ccgccacccc ggccaacgcc ctgcaccgca aggcaaacag ccggcgtggc gcatccgcgt
    11581 ccccgaccgc attatcggtt tttcagacgg catcgtcggc ggttacgccc aaaacctcgc
    11641 ccgcgacatc ggcgatttcg tcctgcttcg tgcagacggt tactgggcat accaactcgc
    11701 cgtcgttgcc gacgatgccg aacagggcgt tacccacatc gtccgcggac aagacctgct
    11761 cgtttccact ccgcgccaaa tctatttgca gcagtgtttg ggcgttccga caccgcaata
    11821 tgcccacctg ccgctgctga ccaacgcaca agggcagaaa tggtcgaaac agacgctcgc
    11881 ccccgcattg gatttaaacc gccgcgaaca actcctccgc caagtgttcc gttacctcaa
    11941 gctgcccgaa gcaccggaaa ccgaccgccc tgccgaactg ctcgactggg cggtggcaca
    12001 ctgggatatg gacaaagtgc cgaaacacgc cattaccacc ccctaaacac cgcccgacaa
    12061 acgcacaaat gccgtctgaa actttcagac ggcatttcac atacagcccc gccctattcg
    12121 cgcgtatttg cccgttcgac ttcttcccac gcttcgagaa tcaggccgcc gtcgttgtct
    12181 ttcaagagca tcgcgtcgga aagcatacgc cgccaagtcc gcgcaccttt caaaccgtgc
    12241 atcagcccga ggctgtggcg gacgatgtgg cgcaagattg tgccgcgtcc ggcttggatc
    12301 tgggcttggc tgtatgtgta gagacgctgc accaaatcgg cgtattcaat cgggctgcgg
    12361 gtatcgccgt aaaacagcct gtcccattcg cgcatcacca tcgggttgtg gtacgcctcg
    12421 cgcccgacca tcacgccgtc aacgtgttgc aggtgtccgg cgattgcttc gttggtggtg
    12481 atgccgccgt tgatgatgat ttccagcccg ggaaactcct gcttgaggcg gtaaacgtaa
    12541 tcgtatttca acgggggaac gtcgcggttt tctttggggg aaagaccgtc cagccaagcg
    12601 ttgcgggcgt ggacgataaa ggttttgcag gcggttttgt cgcgcagcgt gccgacgaaa
    12661 tcggcaacgg tttggtattc ggtctgcctg tccacaccga tgcggtgttt gacggtaacg
    12721 ggaatcttga ccgcatcctg catggcgttg aggcagtcgg caaccagccc gacttcgttc
    12781 atcagacacg cgccgaacga gcctttctgc acgcgcggac tggggcagcc gcagttgagg
    12841 ttgacctcgt tgtaaccgta tgcctcggcg gctttggcgg ctttcgccaa atcggacgga
    12901 tcgctgccgc ccagttgcag ggcgacgggc tgctcgcctt cgttgaacat caaaaagcgg
    12961 tctttgtcgc cgtaaacaat cgcaccggca ttgaccattt cgctgtacag ccaagtattt
    13021 cgggtaatct ggcgggcaag gtaacggtag tgcctgtccg tccagtcgag catcggggcg
    13081 acggaaaggc ggcgcggggg aagggtttgc gtgttgtcgt tcattgtcga tatttcacca
    13141 ttgcggattc aatttcaatc cggaacaacc ttcatttttg gggaaatccg cgtcatgaaa
    13201 gagtccagca gtatggttac cggcccgtcg ttacagagcg acacctgcat atgcgtgcgg
    13261 aaacgccctg tttcgacatg aatcccgtgt ccgcgcaaca gttccgccgt tcgcaggtgc
    13321 ggcttgggaa aacgaaggcc gccgcccgct tgccgcatcg gcataaagtg taaactgcga
    13381 caccagcagc accgcgccgc cgacatcttt caaagacagg ttcaacttgc ccgcttcgtc
    13441 ttcaaacacg cgcaaatggg cgattttgtc ggcgatatag cgtgcatctt tttctgtgtc
    13501 gctatgcgtt acaccgagta gcacgacgaa tccgccgtcg attttgccgc aggtttccgt
    13561 gccgttttcg gacacgacat ccacctttgc acctaccgtt ttctgtatga ccgcacgcat
    13621 aagccgtcct ccttacctga acgtgcaatg ccgtctgaaa aggcttcaga cggcatcggt
    13681 attggaaatg ccattacggt ttgacaccgt tttcttccaa aatggcatcg attttcaagc
    13741 gggctttttc ctgtctcaaa ccttgttcca actggtttct gaccaactcg aaaggctgcg
    13801 cgtcggggtt tttcccgacc tcgctgagtt tgaacagata atagcgttcg cccaatttga
    13861 ccggatcgcg ggtaacgtcg ccccgattca tcgctgcaaa ctgcgaagcc agcggctcgg
    13921 gaagctgctg cgccataatg aaaccgtcaa acgcctgctc gtcgttcgga taacgcttca
    13981 tcagcccttc aaaagacagc cctttgagca ggagctgctg ctcctgacgc gcctcctctt
    14041 cggttgcgaa gctgacctgc tgcaatttga tcatgcggat ttgccgctca taaaactgac
    14101 gcagtgcgct ttcggaaacc gtttccgaac gttccagaaa acggacgtac tcctcggcat
    14161 aaaaagacgc ttcggcgatt ttaaagcggt tttggacatc cttatcctta tccaaacctt
    14221 ccttcaatgc cctgtttttc aaaacttcca aagtttgcag ccgacggacg gcatcgtttc
    14281 ggattgcctg cccgtccggt ttttgggact gctccgcatg ccggtctgcc tgctgcatga
    14341 tctgcgccac cagcgtatcc accaaagccg ggtcgatttc gggtgctttg gctgccgcaa
    14401 aacctgccaa cattgcagca ataactgcgg cagcggtttt tttctgtttc atggtattcc
    14461 ctgtcctgcg cggcggcaaa acgcctgtct gaacgtcttg ccgccatatc ccgttttcag
    14521 aattattttg caggtttgat gtttgccttg cccaacagtg caccgacggc acggtcaatc
    14581 cgttccgcct gaaggttgcc cgcaatctgt cctttcattt catcaaaaga aggcactttt
    14641 acctcgcggc tgtcgttgac ataataaacg ccgtagaaat cgccgttttt cagcggcgtt
    14701 gccgtaaatt cgcctttttt caagtcctta attgcctgat aaagcggcgg aacaccctgt
    14761 tccaaatctt tcagcggcac atatccgacc ggcgcaccgg tctgtttggt acggtcgttg
    14821 agggaatatt gtttcaagac ggcatcgaaa ccttttttcg ccttcaagtc ggcaaccgct
    14881 ttttttgcat tttcttcctt gtcggtcagg atttcgccca actggacttc ctgcgtacct
    14941 ttgtaaaaac cgctgatatt gtcatatgcg gcttttactt cctgctcgga aaccggttgg
    15001 gttttggcga tatgcaatgc gtatgcctcg ccgttcaagc catattttac cgcctgccaa
    15061 acggttttga aggacggttt cttgtcgtcg cccgactttt tcgcttcggc acgcaatttg
    15121 gcaagcgcat ttttaaactc tgccgaccgg tcgagtttca ggcgtttcac ttcctgtgcg
    15181 accacggtat tgaccacttc gttttccagc agggattggc gcagttgcgg cgtgtcttcg
    15241 gcacggctgt tttccgcacg gaatgcggca acctgcgcat cgatgacgga actgtcgatt
    15301 ttctgaccgt tgacggttgc cagcgtttgg gcaaacaggc tgccggaaca ggcaagcagt
    15361 gcaacggaag tcaggatttt tgctttcata atcttctctc tttaaatttt aaattgaaat
    15421 gttcgggcag acttcattca gtccgccgta tggaaatact cgtcaggggt agccgcccgg
    15481 atgccgagcg cgtgaatcat gccgcctgaa aacaaatctt tgagcagcga tttgaccgtt
    15541 ttctggcggt tcaggcggct tacgccttca aaacggccgc taacgaccaa aacggcataa
    15601 tgtccgccgc cggtattgcc cgcgtgtcct ttgtgcagat ggctctcatc gccgatttcc
    15661 aacaccagcg gatcgagcgt ctgcaggcgt tcgcggatca aatcgacggc cggcattatt
    15721 tgaacaccgc tttaaacggc ttgatcagca cttcgctgta cacgcctgca tgaacatagg
    15781 gatcgtcttc cgcccacgcc tctgccgcat ccaaagactc gaactgcgcc acaatcaaac
    15841 tgcccgaaac gcggttggag tcctccggca aaggattcgg gcctgccgtc aacagccggc
    15901 cttccgactt cagcgtctcc agccgtttga ggtgttcggg acgtgccgcc atacgcgcct
    15961 cgtgcacatc ctccccgtct gttgccagca acataaaata ttccacagtc aatcctcctt
    16021 tttcagacag gtactcagat aaataccctg aataataaag aaaacaagca tcagtgcagt
    16081 cgagccgaac attttatagt tgacccattg cgactcgaac cgggtaaaca caaaccagtt
    16141 ggcgataccc ataaaaatca ggaaaccgac ccacatatac gtcaatttcg cccatacggc
    16201 atccggaagc tgaatctccc tgccgatact cgccttcaag ccgtttttac cggcgaggtg
    16261 gctgccccac aggaacagcg cgcccagcca aaacaaaacg ctcggcttcc acataatgaa
    16321 gcggctgtcg cccaaaacaa tggttgcgcc gccgaatacc acaatcagca ccaatccgac
    16381 ccactgcatc gtatccagct ttttatattt ccaatacaga aaagccgcct gaaccacacc
    16441 ggcaaccaag gcaaccgccg tagcggcaat catatttttg gtaacggtat aagtagcaaa
    16501 aaacaagatg acagacaaaa ggtcgctgac gaatttcata tccaaatgag aaagcaaatc
    16561 aaataaacgc taatcataca ggcagaaccc tgtttttgca cctgcaaaac caacaaattt
    16621 tgttttgatt gatgcaaata tgcacagatg tttttgaaaa aagatggaga tatgtcataa
    16681 tttgtaaaaa cggcgaatga ttgccgttta aaatgtggcg gcggtcggta cattcacata
    16741 ttaaatgccc gccggccgac tgccccgccg atgcccgatg atgacgaaat ttacagactg
    16801 tacgcggtca aaccgtattc agccgccaac ccacagggga tacatcttga aaaacaacag
    16861 acaaatcaaa ctgattgccg cctccgtcgc agttgccgca tcctttcagg cacatgctgg
    16921 actgggcgga ctgaatatcc agtccaacct tgacgaaccc ttttccggca gcattaccgt
    16981 aaccggcgaa gaagccaaag ccctgctagg cggcggcagc gttaccgttt ccgaaaaagg
    17041 cctgaccgcc aaagtccaca agttgggcga caaagccgtc attgccgttt cttccgaaca
    17101 ggcagtccgc gatcccgtct tggtgttccg catcggcgca ggcgcacagg tacgcgaata
    17161 caccgccatc ctcgatcctg tcggctactc gcccaaaacc aaatctgcac tttcagacgg
    17221 caagacacac cgcaaaaccg ctccgacagc agagtcccaa gaaaatcaaa acgccaaagc
    17281 cctccgcaaa accgataaaa aagacagcgc gaacgcagcc gtcaaaccgg catacaacgg
    17341 caaaacccat accgtccgca aaggcgaaac ggtcaaacag attgccgccg ccatccgccc
    17401 gaaacacctg acgctcgaac aggttgccga tgcgctgctg aaggcaaacc caaatgtttc
    17461 cgcacacggc agactgcgtg cgggcagcgt gcttcacatt ccgaatctga acaggatcaa
    17521 agcggaacaa cccaaaccgc aaacggcgaa acccaaagcc gaaaccgcat ccatgccgtc
    17581 cgaaccgtcc aaacaggcaa cggtagagaa accggttgaa aaacctgaag caaaagttgc
    17641 cgcgcccgaa gcaaaagcgg aaaaaccggc cgttcgaccc gaacctgtac ccgctgcaaa
    17701 tactgccgca tcggaaaccg ctgccgaatc cgccccccaa gaagccgccg cttctgccat
    17761 cgacacgccg accgacgaaa ccggtaacgc cgtttccgaa cctgtcgaac aggtttctgc
    17821 cgaagaagaa accgaaagcg gactgtttga cggtctgttc ggcggttcgt acaccttgct
    17881 gcttgccggc ggaggcgcgg cattaatcgc cctgctgctg cttttgcgcc ttgcccaatc
    17941 caaacgcgcg cgccgtaccg aagaatccgt ccctgaggaa gagcctgacc ttgacgacgc
    18001 agcagacgac ggcatagaaa tcacctttgc cgaagtcgaa actccggcaa cgcccgaacc
    18061 cgctccgaaa aacgatgtaa acgacacact tgccttagat ggggaatctg aagaagagtt
    18121 atcggcaaaa caaacgttcg atgtcgaaac cgatacgcct tccaaccgca tcgacttgga
    18181 tttcgacagc ctggcagccg cgcaaaacgg cattttatcc ggcgcactta cgcaggatga
    18241 agaaacccaa aaacgcgcgg atgccgattg gaacgccatc gaatccacag acagcgtgta
    18301 cgagcccgag accttcaacc cgtacaaccc tgtcgaaatc gtcatcgaca cgcccgaacc
    18361 ggaatctgtc gcccaaactg ccgaaaacaa accggaaacc gtcgataccg atttctccga
    18421 caacctgccc tcaaacaacc atatcggcac agaagaaaca gcttccgcaa aacctgcctc
    18481 accctccgga ctggcaggct tcctgaaggc ttcctcgccc gaaaccatct tggaaaaaac
    18541 agttgccgaa gtccaaacac cggaagagtt gcacgatttc ctgaaagtgt acgaaaccga
    18601 tgccgtcgcg gaaactgcgc ctgaaacgcc cgatttcaac gccgccgcag acgatttgtc
    18661 cgcattgctt caacctgccg aagcaccgtc cgttgaggaa aatataacgg aaaccgttgc
    18721 cgaaacaccc gacttcaacg ccaccgcaga cgatttgtcc gcattacttc aaccttctga
    18781 agtacctgcc gttgaggaaa atgcagcgga aaccgttgcc gatgatttgt ccgcactgtt
    18841 gcaacctgct gaagcaccgg ccgttgagga aaatgtaacg aaaaccgttg ccgaaatacc
    18901 tgatttcaac gccaccgcag acgatttgtc cgcattactt caaccttctg aagtacctgc
    18961 cgttgaggaa aatgtaacgg aaactgttgc cgaaacgtcc gacttccaca ccgccgcaga
    19021 cgatttgtcc gcgctgctgc aaccttttga aacacctgcc gttgagaaat ttgaagcacc
    19081 ggctattgaa gaaaaagcag cggaaaccgt tgataaaacg cccgacttcc acaccgttgc
    19141 cgacgatttg tccgcgctgc tgcaacctgc tgaagtacct gccgttgagg aaaatgcagc
    19201 ggaaatcact ttggaaacgc ctgattccaa cacctctgag gcagacgctt tgcccgactt
    19261 cctgaaagac ggcgaggagg aaacggtaga ttggagcatc tacctctcgg aagaaaatat
    19321 cccaaataat gcagataccg gtttcccttc ggaatctgta ggttctgacg cgccttccga
    19381 agcgaaatac gaccttgccg aaatgtatct cgaaatcggc gaccgcgatg ccgctgccga
    19441 gacagtgcag aaattgctgg aagaagcgga aggcgacgta ctcaaacgtg cccaagcatt
    19501 ggcgcaggaa ttgggtattt gattcccaac tgccctttcg cagatcaagg atgccgtttc
    19561 agacggcatc tttttttgcc ttatcggtgt aacggataaa gtttgaaccg gcacaggctc
    19621 aaacagcagg tcgacggcaa caaaatgccg tctgaaaccc ctaaaggctt cagacggcat
    19681 tggcggcggc ggtatcaggc agtttgatag gcaaccgaat cgtcgccgct tttcttttga
    19741 atgaactcga ttttgtatcc gtcggggtct tcgacgaagg ctatcacggt tgtgccgtgt
    19801 ttcatcgggc cggcttcgcg gacgacgttt ccgccctgcc gcttcacacg ttcgcaggct
    19861 tcgtaggcat cgtccacttc aaccgcgatg tgtccgtagg cgttgcccaa gtcgtatcgt
    19921 tccgtatccc agttgtgcgt cagttccaaa accgtgctgt cggtttcatc gccgtaaccg
    19981 acgaaggcaa gggtaaatct gccttcggga taatcttttc ggcggagcag tttcataccc
    20041 aaaacgtttt ggtagaaatc gagggatttt tcgagattgc ccacgcggag catagtatgg
    20101 agtaagcgca tttttgtgtt cctctcggta gtggttaaac ttcgattttg tttggcgtaa
    20161 acgtctgcca tttgttgcag gcaggacaat gccagaaaaa gacttgtgat ttgaagtggc
    20221 agtttcggca ccggtacatc acgctgcgct gtagctgccg tccgataacc gaacgcatca
    20281 tgtcggcatc ggctttccaa gccggattca tatcgctgag tttcaaaccg agcaggcggt
    20341 acacgccgtt aaggtcgggc ttgcggcgga caagctcgac ggcggtttgc gcggcttctt
    20401 tctcgccctt aagcagcagg gatttctcgt acacgacatt gatcaggtca agttcgggaa
    20461 acgtctgcat atatcctgtc agacggttca agccttcttc aggttttccc tgcgcggcat
    20521 aggcttcgta aagcttctcg ccgaccatgc tcaagtatgc atggttttgc tgctcgatgg
    20581 cggcataggc ttcgacggcg gcagggaaat tgccttgtcg gtgttcgatg tcgcccaaaa
    20641 tcatgttggc gcgggtgcat tttttgttgg cttcgagtgc cttgccgaca ttgaaacgcg
    20701 cggcatcgaa attggacttg aacagcgcgg cttgggcaag ttcgcaatag aactgtgcaa
    20761 tctcaaactg ataggtctgc tcgtcatggc tgagcagccg ggcggtttca accgcttttt
    20821 cccaatccct gtcctgttgg tagatattga gcaggtgctg tctggcttca cgcgccattt
    20881 caccgtcttg cagccccaaa aaaatctgtt cggcacggtc aaccaacccc gcactttgat
    20941 agttttgcgc caattcaaat aatacgcgcg cacgcttggc tccggttgta tcgggagagt
    21001 caagcaatgt ttggtgcata ttgatggctt tgtcgttttc gccacgctga cggtaaagct
    21061 tgcctagggt gaggttcagg tcgtatgact gaggctgctg atcgatgacc tctgccaatt
    21121 ccctggcggc acgtccgctg ttgcggtcaa ccaaggcatc cagactttta taaaatcccg
    21181 acggtatgct ttttgcctgc tttaatacag tcttcatatc cacgcgggcg gcaaaccagc
    21241 ccatcgcgaa gaaaacgggc aaaaggataa tcggcagcag gataatccac aattcattgt
    21301 ccatatcggc tttctcaagg ctgtttggca gattcgggcg cattttgcgc cggtggtgcg
    21361 gtcagctcct tccccgtcaa acgcgcattt ttctttactt cggcacgcaa cctgccgttc
    21421 tcgccacgta acgacaacaa ccgtccgaac aaggcaaaca ttccaaaaac aataccgact
    21481 acaaatgcgc cgaacaatac gacaatcagc ggcaaatcga atttttgccc cggcaggtag
    21541 gaaaaggtaa cggcatccgt attaatgaca gcaagcagca ggaagagcag caggataatg
    21601 attttgatga cggtatagat aagtttcatg tttgctccgt aaataaaaac gccgcccgga
    21661 cagcttcgca ctctgaacca agattgcgct agtttaaacg atttgcacgg tttcggatag
    21721 gatgcggcag cgtgttcgga cgacatacgg agtatgcgat tgccgacaat tttaccaaat
    21781 acaccgttcc tttccatttg aaaaataacg gattggacac cgcatcgaca gaaaaacccg
    21841 ccgcgcactt gtcaaaaccc tgttggcagg cgtatcttta caatcttcaa attcaaaccg
    21901 ttcattgaaa catatcagaa taagaaaggc tttacatcat gagcagaccc gtacccgccg
    21961 tattcggcag cgtttttcac agtcaaatgc ccgtcctcgc ctaccgcgaa ggcaaatggc
    22021 agccgaccga atggcaatct tcgcaagacc tcaccctcgc acccggcgcg cacgccctgc
    22081 actacggcag cgaatgcttt gaaggcttga aagccttccg tcaggcagac ggcaaaatcg
    22141 tgctgttccg tccgactgcc aacatcgcgc gtatgcggca aagtgcggac attttgcacc
    22201 tgccccgccc cgaaacccaa gcctatctcg atgctttggt cgaactggtc aaacgcgccg
    22261 ccgatgaaat tcccgatgcg cctgccgccc tgtacctgcg tccgacctta atcggtaccg
    22321 atcccgttat cggcaaagcc ggttctcctt ccgaaaccgc cctgctgtat attttggctt
    22381 cccccgtcgg cgactatttc aaagtcggct cgcccgttaa aatcttggtg gaaaccgaac
    22441 acatccgctg cgccccgcac atgggccgcg taaaatgcgg cggcaactac gcttccgcca
    22501 tgcactgggt gctgaaggcg aaagccgaat atggcgcaaa tcaagtcctg ttctgcccga
    22561 acggcgacgt gcaggaaacc ggcgcgtcca actttatcct gattaacggc gatgaaatca
    22621 ttaccaaacc gctgaccgac gaatttttgc acggcgtaac ccgcgattcc gtactgacgg
    22681 ttgccaaaga tttgggctat accgtcagcg aacgcaattt cacggttgac gaactcaaag
    22741 ctgcggtgga aaacggtgcg gaggcgattc tgaccggtac ggcagccgtc atctcgcccg
    22801 ttacttcctt cgtcatcggc ggcaaagaaa tcgaagtgaa aagccaagaa cgcggctatg
    22861 ccatccgtaa ggcgattacc gacatccagt atggtttggc ggaagacaaa tacggctggc
    22921 tggttgaagt gtgctgatgc tttaaataaa aaatgccgtc tgaaacccgt ttggcgtttc
    22981 agacggcatt ttcgcatccg aaccgtttcc gctgcacctg cagcaagtcg gcacaaaggc
    23041 aatcggttaa aacaagcgtc cgcatttccc atcccgcctg ccgtaagtcg ggcatttctc
    23101 tagaaatacg cttcagacgg caaaccgccg cccgaaaccg atatgcggta cggacggcgc
    23161 acggatttga aaacggcgga ttatccctct gtgctcaagg cattgatgct gtaaccgccg
    23221 tcaacgtaag tgatttcgcc ggtaatgccg gacgacaggt cggacagcag gaaggcggcg
    23281 gtattgccga cttcttcgat ggtaacgttg cggcggagcg gattgtgggc ggcgacgtgt
    23341 cccaagagtt tgccgaaatc ggcgatgccg gaggcggcaa gcgttttgat cgggcctgcg
    23401 gaaatgccgt tgcagcggat gccttctttg cccagacagg cggcggtaaa acggatgccc
    23461 gcctcaaggc tggcttttgc catacccatc acgttgtaat tcggaatcgc gcgcaccgcg
    23521 cccaagtagc tcagggcaac gatggcggag tttctgccgc gcatcatcgg acgggcggct
    23581 tttgccaatg cgggcaggct gtatgcggaa atttcgtgtg cggtgttgaa cgcttcgcgg
    23641 ctgatgctgt cgaggaagtc gccgctcaag gcttctttcg gcgcaaaacc gatggaatgc
    23701 accaaaccgt ccaagccgtc ccaatgtttg cccaaatcgg cgaacacttg gttgatttcg
    23761 tcgtcgctgg cgacatcgca gcggaacacg agttcggaat ccaattccgc cgccattttg
    23821 cggacgcgct cttccagttt gtccacaacg taggtaaacg ccagttccgc gccttgttcg
    23881 cggcaggctt tggcgatgcc gtaagcgatg gaacgctcgg aaatcatgcc ggtaatcaga
    23941 atttttttgc cttgcagaaa acccattttg tatccttcaa atagtgttcg gttcgttcta
    24001 aaccgttgat tatagcaaat tgtccctgtt tctgtgtttt cacgttgcag catgcaaacg
    24061 gcaatgccgt ccgaagcgga tttcagacgg catcggacgt ttcaaatacg gtttaaggca
    24121 tcagatgccg cgcaacaatt cgttcacgct ggttttcgca cgggtttgcg cgtccacgcg
    24181 tttgacgatg acggcgcagt aaaggctgtg gctgccgtct ttggaaggca tactgccgga
    24241 cacgacgacc gaacctgtcg gtacgcggcc ttggtagatt tcgccggtgg tgcggtcgaa
    24301 gattttggtg gattgaccga tgaacacgcc catcgaaatc acgctgcctt cttcgacaat
    24361 cacgccctca acgatttcag aacgcgcacc gatgaagcag ttgtcttcaa taatggtcgg
    24421 gctggcttgc aggggttcga gtacgccgcc gatgccgaca ccgccgctca agtggacgtt
    24481 tttgccgatt tgcgcgcaag agccgacggt cgcccaagta tcgaccatcg caccttcgtc
    24541 aacgtatgcg ccgatgttga cgtaagacgg catcagcacg acatttttcg ccacaaagct
    24601 gccgcgtcgg gcaaccgcac ccggaacggc gcggaagcct gcgtttttga actcgtcttc
    24661 agaccagtcg gcaaacttgg tcggcacttt gtcgaagtat ttgttcacac cgtcgttgag
    24721 gatttcgttg tcttggatgc ggaaggacag caacacggct tttttcgccc attcgttaac
    24781 tttccattcg cccacgccca agcgttcggc gacgcgcagt ttgccggaat cgagttggcg
    24841 gatggtttcc aacacggctt ctttaacttc gggagtaacg gtggtcggat taatgtccgc
    24901 gcggttttca aaggcggttt cgataatgtt ttgcaaagac ataatgtttc cttatgtgag
    24961 atgtttcgtg ggacgcttgc cggtattttc aagcagtatg ttcatttgaa atgaatgcgc
    25021 tattgtgcca cgcaaacgca tattcgtcag caatacgcgg gcggttgccg aacattggcg
    25081 tttcggaatc gggcgggcgg cagtaccttt atgccgcacc gccgcctttc agacggcatt
    25141 cgcggtaaaa cgccatcagc ccttcggtcg aggcatcgtg tacggacgtg ccgccttcca
    25201 gttcgccgat gatggttttc gccaactgtt tgccgtattc caccccccac tgatcgaagg
    25261 ggttgacgtt ccatatcgcg ccttggacga aggttttgtg ttcgtaagcc gccatcagca
    25321 tacccaaatt gtagggcgtg aggcggtcga gcaggatgct gttgctgggg cggttgccag
    25381 ggaactcttt gtgcggcgca aggcgttcgc gttccgcttc gggcaaatct gccagttcgg
    25441 cgcgtgcttc gtccaaggtt ttgcccttca tcagggcttc cgcttgggca aaggcgttgg
    25501 caacggtaaa acggctgcgt ccgtcctcca cgccctgcgc cgtcaccggg acgataaagt
    25561 cgcagggaat caggcgcgtg ccttgatgaa gcagttggaa ataggcgtgc tggcagttga
    25621 ccccttcacc gccgaacacg atgccgcccg ttttgcacac ggcgggactg ccgtctgaag
    25681 cgcggctttt gcccaaactc tccatatcga gttggttcag ccacgccggc agcaggcgca
    25741 ggttgtggct gtacggaacg gcggtctgcc cgtccgcgtg ttggaaattg ttataccaca
    25801 cggcaatcag tgccatcaga acgggaatgt tgcggcgcgg cggcgtgtgg aaaaaatggc
    25861 tgtccatcgc gtgcgccccc gccaacaact cgcggaaacg cgccccgccg accgcaacca
    25921 tcacgggcaa accgacgggc gaccagacgg aatagcgtcc gcccacccag tcgtacatcg
    25981 caaacacgcg ttccgccgca atgccgaaac tttgcgccgc ttccgtatcg gcagataccg
    26041 cgcaaaaatg acatcccgtt tccgattccg agaaccccgc accacgatac caagccttga
    26101 ccgcctctgc attgagcagg gtttcaggcg ttttgaagga cttgctggca acgcaaaaca
    26161 ctgtcgtttc ggggttcaga cggcataaaa ccgcatccag gcaggcagga tcggcgttgg
    26221 cggcaaaatg gacggcgata tgccgtctga acggctcaag tgcctgcacg cacattgccg
    26281 gcccgaggtc ggatccgcct atgccgatgt ggacaaaatc cgtaatccgt ttcccagtta
    26341 tcccctgata cgaaccgtcg tccaaactgt gtgcaaactc caacgcacga tttaactcgc
    26401 ggcggatttc gggcaacacg tccctgccgt ccacataaac ggcatccgca ccgtcgggca
    26461 ggcgcaaagc cgtatgcagc gcggcacgcc cctcgctgcc gttgactttc gcacccgtcc
    26521 gcaaagcacg cattttccct tccaaatccg ccgtctccgc aagacggcag agcagttgca
    26581 gcgtatcttc gcccaaacgg tttttgctgt aatcgaacaa catcccgtcc aaacgctcat
    26641 gcatacgctc aaaacggtcg ggttcgcagg caaagcggtc gcgcaaaagg acatgacgcg
    26701 tatcctgata atggcgttcg agcgcatacc atgcacgggt aaaagcattc atctgttttc
    26761 cttgattttt cagaaccgga ttaaaatgta gcagaatgta gtttaacaaa cggcagcggc
    26821 tttggcgaat ctccagaaca ccgcccgccg cgtccaatag caccttattt ccatatggcg
    26881 gaaatgccct ccggccggca tttgacccgc aacaatatcc tgcaagattt attgtgtacg
    26941 cataaatgcc ggacagccgc ctaaataagg tataatcgca tccgattctg tcccgtttgc
    27001 cgcccgcagg cgggatgtcg gtggttttca tcttaccggg aacacgccgg tactgttttt
    27061 taacgatgtt tcttacattt tattccaatt actttacggg gctagaatat ggctaaaaac
    27121 ggaggatttt ctttgttcgc aaagaaagaa aaacgcttta tctttgaagg caggcattcc
    27181 gcctccgaca aactggtcaa cggcgaagta tccgcgttta ccgaagaaga ggcgcgcaaa
    27241 aaactggcaa aacgcggcat ccgcccgttg cagattactc gtgtgaaaac aagctccaag
    27301 cgcaaaatca cacaagaaga catcaccgtt ttcacccgcc agctgtccac gatgattaaa
    27361 gcgggcctgc cgctgatgca ggcatttgaa atcgtggcgc gcggacacgg caacccgtct
    27421 atgacggaaa tgctgatgga aatccgaggc gaagtggaac agggcagctc gttgagccgc
    27481 gcattctcaa accacccgaa atatttcgac cgcttctact gcaacctggt tgcggcaggc
    27541 gaaacgggcg gcgtattgga aagcctgctg gacaaattgg caatttacaa agaaaaaacc
    27601 caggctatcc gcaaaaaggt aaaaaccgca ctgacctatc cggtatccgt catcgccgtc
    27661 gccatcggtt tggtattcgt gatgatgatt ttcgtactgc ccgcctttaa agaagtttac
    27721 gccaatatgg gcgcggagct tcccgcactg acccaaacag tgatggatat gtccgacttt
    27781 ttcgtctcat acggctggat ggtgctgatc gcactgggct ttgccatata cggcttcctt
    27841 aaattgaagg cgcgttcgat taaaatccaa cggcgtatgg atgccatact gctgcgtatg
    27901 ccgattttcg gagacattgt ccgcaaaggg acgattgccc gctggggcag gacgacagcg
    27961 acgctgattg cggcaggcgt gcctttggtc gatgtattgg actccactgc cggcgcggcg
    28021 ggcaatttaa tctatgaaga agccacccgg gaaatccgta cgcgcgtcat ccaaggtttg
    28081 tctatgactt cggggatgcg tgcgacggaa ctgttcccca atatgatgtt gcagatgtct
    28141 tccatcggcg aggaatcggg ttctttggac gatatgctca acaaagccgc cgaattttac
    28201 gaagacgagg tggacaatgc ggtcggcagg ctgtccgcta tgatggagcc gatcattatc
    28261 gtgattttag gcttggtcat cggcacgctt ctggtcgcca tgtatctgcc gctgtttaat
    28321 ttgggcaacg tggtcgcctg atttgccgca cagatccggc gcggattcgc tctgcgccgg
    28381 tttgtttttg ttttgaatac attgagaaca aaatatgtct gatttgtctg tattgtcgcc
    28441 gtttgccgtg cctttggcag cggtgttcgg gctgctggtc ggaagtttct taaacgtcgt
    28501 catttaccgc gtgccggtca tgatggaacg cggctggacg gtatttgcca aagaatattt
    28561 aaacctgccg ctgaccgaag aggaaagccg taccttcaac ctgatgaaac cggattcctg
    28621 ctgtcccaaa tgccgcgtgc cgatacgcgc gtggcagaac atcccgattg tcagctacct
    28681 gctcctgcgc ggcaaatgcg cttcctgcca aaccaaaatc agcatacgtt atcccttaat
    28741 cgagctgctg accggcgtat tgttcgggct ggttgcctgg caatacggct ggtcttggat
    28801 tacattgggc ggtttgatac tgaccgcgtt tctgatttcc ctgaccttta tcgatgcgga
    28861 cacccaatac ctgcccgact cgatgacact gcccttgatt tggctggggc tgatatttaa
    28921 tttggacggc ggcttcgtgc ctttgcagtc tgccgtttta ggtgcggttg ccggctatgg
    28981 ttcgatatgg cttttatgcg cggtatataa actgattaaa ggcgaaatcg gtatgggcaa
    29041 cggagatttc aaactgattg ccgcattggg cgcgtggctc ggcatatccg cattgcccgt
    29101 gctgattttt gtttcctcgc tgatcggttt ggtcgcggca atcgttatgc gcgtcgccaa
    29161 ggggcagcat tttgccttcg gccccgcact gacagtttcg ggctggataa tttttacggc
    29221 aaacgattcc gtatggcggg cggtcaactg gtggctgacc catccggtgc tgtaagatga
    29281 cggtatgggt cgggctgacc ggcggaatcg gcagcggcaa atcggcagcc gcgcaatgtt
    29341 ttgccgattt gggcgtaccg cgtatcgatg cggatgcggc ggcgcactcg ctgacggctt
    29401 cagacggcat cgccctgccg gaaatcaggc ggctgttcgg cgacaccgtt ttcgacacac
    29461 agggtttgtt gcggcgcgac atattgcgta aagaaatctt tgcctcccca tcgcgaaaag
    29521 ccttgctcga atccgtgatg ttgccgctga ttttctcaga aatcaaaaaa cagcaagaaa
    29581 cctttaccga tgcggcttac ggcattgtcg aaattccgct gctgacggaa aagcgtcaat
    29641 ttatcagcct gatacggcgt gtcctgacca taagtgcccc tgtggaaaaa cgtatcggca
    29701 gggtgatggc ccgcagcggg ctgacgcgcg gcgaggtggc ggccgtcatc agccatcagg
    29761 catccgaatc cgaacgcctg ctgcttgcag acgatgtgct gctcaatgac ggcagcctca
    29821 aaagcctgcg tgagaaaaca atgcgcctgc acgcgtttta ttcagggatt ttcgcctcaa
    29881 aaccaacaca aggaaaacac aatgactgaa tcgcggcaaa cacgccttca agtcaaatgt
    29941 ccgacctgtc aaacagcagt agtatggaaa cccgaaaacg cattccgccc cttctgttcg
    30001 caacgctgca aactgatcga cttgggcgga tgggcagacg ggaaatatac ggtttccggc
    30061 caaacggaaa gtttgccgga aatatccgaa cccgacatgg cataccgctg accgccccgc
    30121 cttcccggca aacaccctga aagtcaaatg ccgtctgaaa caaacacgct tcagacggca
    30181 ttttcattct caaacctaat cgttggtatt tgccgttacc tcttccaatg aagtaatgcc
    30241 ctgcataact ttcaaaatac cggcccggcg caaatccacc ataccctcct tataggcaac
    30301 gtccaaaata tccacttccg taccgttgtt cataatcaca cgctgcattt cttcgctgat
    30361 gggcataacc tcatacacgc ccgcacgccc cttataaccc tgcccccggc aacggtcgca
    30421 accgacggcg cggtaaagtt tccaatcttt tgcaaggtcc tcatcggtga agccgacttc
    30481 cttcaaagca gaggcagacg ggcgttccac ttcctgtttg cagctcgaac acagcctgcg
    30541 taaaagacgc tgcgccataa tcaggctgac cgaactggca atattaaacg gcgcgacacc
    30601 catattcagc atacgtgaca acgtcgccgg cgcattattg gtgtgcaggg tggaaaacac
    30661 catatgccct gtttgtgccg ccttaatcgc aatatcggca gtttccaaat cacgaatctc
    30721 accgaccata atgatgtccg ggtcctgacg caggaaagac ttcaaagcag cggcaaaagt
    30781 caggccctgc ttatcattga cgttaacctg attgatgccc ggcaggttaa tctcggcagg
    30841 gtcttccgcc gttgcaatat ttaccgactc cgtattcaaa atattcaaac aggtatagag
    30901 cgacaccgtc ttacccgaac ccgtcggacc ggttaccagc accatcccgt agggacggtg
    30961 aatcgcttcc aacaacaatt ttttctgaaa cggctcaaaa ccgagctggt cgatgttcaa
    31021 agacgcggca tcggaattca aaatccgcat cacgaccttt tcgccaaaca gcgtcggcaa
    31081 tgtgctgaca cggaaatcga caggcttgcc gcccttttga aaggtcagct gcatcctgcc
    31141 gtcctgcggt atccgttttt cggaaatgtc caaacgcgac attaccttaa tccgggaagc
    31201 aagctgcccc cttaccgcaa tgggcggctg aaccacctca cggagctgcc cgtccacacg
    31261 gaaacggata cgggcattgt gttcgtaaaa ctcgaaatgg atgtccgatg ccccgctgcg
    31321 caaggcatcc gacaaagtct tatggataaa cctcggaaca gggccgtctt ctgcctcctc
    31381 gttgtcgata tacagggtgt ggctttcctc ttcctcctgc ccctccccaa gctcctgaag
    31441 cagcgatgtc gaacgcgaac ccacccaatc gagcaaaccc gccaactggt catcctcgac
    31501 aatgaccaat tcaacctcaa tccctgcggc agaaacggtt ttctgaattt gcggcatctg
    31561 tgtcggatcg gaaaccgcaa aaaatacttt gtcgccccga cggaaaaccg gcacacagtg
    31621 gaactccacc atctgctcct ccgtcaacac ccccatcagc accctgtggc gcggataatg
    31681 acgcaaatca agaatcgaat aactgaacac cctcgcaatc aatgccgcaa gcgacttggg
    31741 cgaaatgaca ccgtctgaaa acagcatcgg caacacttcc ttacccgcct gcgactcatt
    31801 gtagtaatgc tcggcctgct caacagtaac cacctggttt tgaaccagaa tcctcagcaa
    31861 acctacgctc atacgacctt atccccaaat ttattcattg ttatacctgt acag
//
LOCUS       DAXHSJ010000003        30118 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000003 DAXHSJ010000000
VERSION     DAXHSJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.003"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(161..1534)
                     /locus_tag="ACFM8U_000114"
     CDS             complement(161..1534)
                     /locus_tag="ACFM8U_000114"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC6333430.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKDYVLTLLFLKYVSDKHKQGGGMIELPAGTTFDDIVKLKNTADIGDR
                     LNKIIAQIAEANDLKGVIDVADFNDEDKLGKAKEMIDRLSRLVGIFEKLNLSSNQAED
                     DDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSKIIGISADCPSSTSVYDPTCGSGSL
                     LLKAAAQAGSQISLYGQEKDVATASLARMKMILHNNETAEINTGTPCPIRLSVMKTTG
                     LRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAIILPHGVLFRGNAEARIRTE
                     LLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQFAEEGTNQVISGGSVFMID
                     ASRGFIKDGNKNRLREQDIHKIIDTFTKQEEIPRYSRMVHLSEIAAQDYNLNLPRYID
                     SGEVEDL"
     gene            complement(1531..2190)
                     /gene="ung"
                     /locus_tag="ACFM8U_000115"
     CDS             complement(1531..2190)
                     /gene="ung"
                     /locus_tag="ACFM8U_000115"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC6333431.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            2271..2678
                     /locus_tag="ACFM8U_000116"
     CDS             2271..2678
                     /locus_tag="ACFM8U_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC6333432.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            2693..3640
                     /locus_tag="ACFM8U_000117"
     CDS             2693..3640
                     /locus_tag="ACFM8U_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC6333433.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(3747..4817)
                     /locus_tag="ACFM8U_000118"
     CDS             complement(3747..4817)
                     /locus_tag="ACFM8U_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC6333434.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            5002..5277
                     /locus_tag="ACFM8U_000119"
     CDS             5002..5277
                     /locus_tag="ACFM8U_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC6333435.1"
                     /translation="MTEQENKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            5279..5902
                     /gene="lipB"
                     /locus_tag="ACFM8U_000120"
     CDS             5279..5902
                     /gene="lipB"
                     /locus_tag="ACFM8U_000120"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC6333436.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLVGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            5895..6863
                     /gene="lipA"
                     /locus_tag="ACFM8U_000121"
     CDS             5895..6863
                     /gene="lipA"
                     /locus_tag="ACFM8U_000121"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC6333437.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRECEF"
     gene            6926..8668
                     /locus_tag="ACFM8U_000122"
     CDS             6926..8668
                     /locus_tag="ACFM8U_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333438.1"
                     /translation="MDQQPVIEIAEFPHDNKLWRIDGLGKIFSQLKNNNNLFIEVYLR
                     PVNFHDNSVYNFAKFDSSGREIPEYNQLKKIPVNIGLLPILRVGSLWIKGKLQNNNAV
                     TDIATLKNIEINANTVEIINSNYKIKYLDSSGREQECKLLLQKNFATDAKVPFSCFKI
                     QNENYKNGIIIPVIEIIRFYYACSTRLAHLTFSNPDEITKISYAIDNETDKVVIKLPQ
                     QYTDQEAFILARILTSNIALSGLFQIKNSLIKIGVNEQPTTLLSTNFPFINPTNLMVR
                     GIKIVDRFLVTEICSCSAPFPDISVDRNNDNRKGDKKTDIPDDKKKEYRRGGQTPSLK
                     GNSTIQNQYESVNCVSTLQFNLNINCFSDLIGKKIEKPQKPFNEYKAQKLAINEKINV
                     TSLGVDLGTWSQTTIVPASISTFNTQSEKQNRDALAATFDNSVRAIEHLNEEYSDIQA
                     TIYMSSSRACEFVPLTDSAHKAQWSYLVSKTKTRRKVLLGKIEYKDKTFWLIEIQARK
                     SEHFVTAIIYFEAIDETKYLTQLLYVLAKQRGIWDKVPKNSSLQIITLKHTHKDIKSY
                     AQKIHKKISNFILK"
     gene            8940..10064
                     /locus_tag="ACFM8U_000123"
     CDS             8940..10064
                     /locus_tag="ACFM8U_000123"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFC6333439.1"
                     /translation="MKNRDTPHKKFINPTEYSLFAEEEQYDFELMIENQIKEHEKEID
                     YYVTDYPIETLVAKLDRQELFIPDYQRKNIWEKHRKSRFIESVLIGLPIPFLFFWQDP
                     STGNMEVVDGAQRLTALSEFIHGNLKLSYLEKLIYLNGQTYHQLLESRQKIFKNKPIR
                     GVVLSNKTDKEARLDLFERLNTGSKNATPAEIRRGLLQGAFYDMVETLSEDKTFNELA
                     KISKNMQDMGEREEFITRFFAYGEYLDHYSANVRKFIFDYTEIMNEEFAKNPNMRATY
                     EQKFHEIMAFFKEKYPIGFKRDGKGQSSRTRFEGMAVSAYLAEKENPGILAQVTPKEC
                     VDIFDSKEFIEVTGSDGANATKKLHARLFFAKNAFKRNHP"
     gene            10061..10813
                     /locus_tag="ACFM8U_000124"
     CDS             10061..10813
                     /locus_tag="ACFM8U_000124"
                     /inference="COORDINATES: protein motif:HMM:NF037890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAE_28990/MAE_18760 family HEPN-like nuclease"
                     /protein_id="HFC6333440.1"
                     /translation="MNSVKSMMESREEEINLYMDLLKYIHDSTQHGLPALQNRNGVEY
                     QLENTHLHILTASIYMLLYNYIEAVVTQSLNTLICYIQNERMPITQFSEPIQKEWLIG
                     GLNLNDAKASVETRLGNGLKMLKHFGDTQKIDIKISKGGGNFNDDEIFKLAKKLQVNM
                     CIDDETHRQLRQAGNITYESTRKFQDTCMKEITRERNKLAHGEITFQECGRNRTISDL
                     MAIKDTTFRYMHLFTDAFTEYLMQKKYLKPTV"
     gene            complement(10797..11819)
                     /locus_tag="ACFM8U_000125"
     CDS             complement(10797..11819)
                     /locus_tag="ACFM8U_000125"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6333441.1"
                     /translation="MNIEAVDLFCGAGGLTAGLLRAGIKVKAGYDIDAACAYAYEYNN
                     KGAKFILQDVETVTGDQIQAHYSKDAVRLLAGCAPCQPFSTYNQGRDVRNDKKWPLLY
                     AFSRLIHEVQPELVTMENVPDVVKHEVYQDFVNNLKQQGYQVWAEKVLCADYGVPQSR
                     RRHVLLASRIGNIQMVPPTHSNCHVTVRQAIGHLPPLAAGKQDTIDVLHRCSSMSARN
                     LERIRASRPGGTWMDWPEDLRAACHRKKKGQSYKRIYGRMEWDKPAPTMTTLCIGFGN
                     GRFGHPEQDRAISLREAALFQSFPLDYQFTQETDTPIRQISRMIGNAVPVLLGEAIGN
                     SFKLLV"
     gene            complement(11880..12905)
                     /locus_tag="ACFM8U_000126"
     CDS             complement(11880..12905)
                     /locus_tag="ACFM8U_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HFC6333442.1"
                     /translation="MILPAHPKPYPDELFTSWLVRLAHANGLKVQTFCHLLFPNYEIW
                     NRDIDRHTPDWLVNTLAEKTGCSTIRIKTTTLDSLQGRLFDKNNFSGQLTWINSLKAN
                     HRKRDNNAIVYCPLCLATDHETYFRKSWRIALYTFCPIHQIMMHDCCPKCGIYIAFHR
                     QDLGKPNLQRFTELKYCWNCQYDLSNSPCENVIFFNDEIRLQWQNWLNQIANLDYLFN
                     QSEKEQLKILHHFLAITTSKKLAPDLYSYLCKQIHQIPVQLERSRRLSWESRSLAERH
                     STIHACLWLIQNFPKNLFKAWQDKAVRYNHLLKDFNDCPQSFQEIILKMNRNVHKELY
                     YSELIYY"
     gene            complement(12902..13795)
                     /locus_tag="ACFM8U_000127"
     CDS             complement(12902..13795)
                     /locus_tag="ACFM8U_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HFC6333443.1"
                     /translation="MNYPHLSPKAKEQLKYSDIERIEYIRSPRWIGYPQAQQVLVKLE
                     DLLSFPKQNRMPNMLLVGDTNNGKTMLIKHFLRQHPAHDNPHGNGIIAPVVIIQAPPI
                     PDESRFYNAILDLLFAPYKTHDRTDKKLAQVLHLLKYVNTKMLIIDEIHHILSGSLNK
                     QKTFLNVIKYLGNELQIPIVGVGIKDAYRAIQTDAQLANRFEPAVLPRWELNKDFMRL
                     LMSFERMLPLSQPSDLHEISLATKLYGMSEGYIGELSRLLTQAAVAAIQQQTECINET
                     ILNSINWISPSQRKRQLDKLR"
     gene            complement(13792..15654)
                     /locus_tag="ACFM8U_000128"
     CDS             complement(13792..15654)
                     /locus_tag="ACFM8U_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFC6333444.1"
                     /translation="MKATLNVNVGAMVLFQNKICVITAVLELETYQLRDSVTGADYQA
                     KLADLSTVQSAELTHLDSIPEQKWQLAQQHFYVIEPLIRNPKRSKSDVQARAAECGVH
                     ISTVYRWLKSYKESGVLTALTRKQRSDSGKTQLSDDVEQLIRQVLEVDYLSTQRKSIQ
                     KIIREITRRCHKQNLPAPHANTIRSRIKNIAPQIKIAKRLSHKAAEMKFSPLQGSFPN
                     ADYPLSVVQIDHTKLDIILVDDIYRKPIGRPWITLAIDVFSRMVTGFYVSFDPPSALS
                     TGLCLAHSILSKESWLAKHGVKGRWPVWGLPRKIHLDNAKEFRGKVLEKACKQYGIEI
                     EWRPVARPHFGGHIERLLGTILDEVHGLTGTTFANTQQRKDYASESKAALTLTEFETW
                     LTLFICDVYHQRTHSGLGISPLAKWKSGVLGDDVSLGTGLPDKVVDETLLRLTFMPFE
                     MRTVQQYGVVIHKITYWHDVLRPWIAATNPTNPRQARQFIFRIDPRDLSVIWFYDPDL
                     SMYFPIPYRNSSNPVISIWELREIQRQLKNEQKQNIDENMIFTAYGRMRELEQQAQGK
                     TKAIRRAQQRRQLDQRTRASLPMPQIDSRNIISSENEENMFDDIQPFDDLDDLS"
     gene            complement(15651..16298)
                     /locus_tag="ACFM8U_000129"
     CDS             complement(15651..16298)
                     /locus_tag="ACFM8U_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HFC6333445.1"
                     /translation="MPIRKIPKNYRNITGIAPYNKAIGIAAYESSLERDFLTLLEFDS
                     NVQHFEVQPITIEWFDPAGKKHIYTPDVLVHYQNDVIIYEVKYRSDLRKNWCELKPKF
                     QAALHFCKLHGWHFKLITELEIHTDYLCNVRFLLPYQQNGLSGEYSEAYMTLLDESLR
                     ELKKSTPKQLIQQTFQDEYNQAKLLPVLWYLIAERKIGADLNQPLTMQSAIWFKK"
     gene            16510..16668
                     /locus_tag="ACFM8U_000130"
     CDS             16510..16668
                     /locus_tag="ACFM8U_000130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333446.1"
                     /translation="MEFTAKATAVAGIIKPGTPRQSEERFPAPAAPFDVNNINLNCPC
                     RLKALQTA"
     gene            17021..17485
                     /gene="bfr"
                     /locus_tag="ACFM8U_000131"
     CDS             17021..17485
                     /gene="bfr"
                     /locus_tag="ACFM8U_000131"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6333447.1"
                     /translation="MQGNQAVLDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMVRAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            17513..17986
                     /gene="bfr"
                     /locus_tag="ACFM8U_000132"
     CDS             17513..17986
                     /gene="bfr"
                     /locus_tag="ACFM8U_000132"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6333448.1"
                     /translation="MKGDRLVIRELNKNLGLLLITINQYFLHARILKNWGFKELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(18026..18325)
                     /locus_tag="ACFM8U_000133"
     CDS             complement(18026..18325)
                     /locus_tag="ACFM8U_000133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333449.1"
                     /translation="MLPIICFRMNVKVKHLLLRILLHIKAKKKGWRQPHPNTHTHQEE
                     RGKIKTDWETANKQSRFHIGIIKQKPLSSIDSNQFRFDSQTGKEKRHTRLYGYAV"
     gene            18312..18632
                     /locus_tag="ACFM8U_000134"
     CDS             18312..18632
                     /locus_tag="ACFM8U_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6333450.1"
                     /translation="MMGNKLTLPAELPAEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            18681..21239
                     /gene="glnD"
                     /locus_tag="ACFM8U_000135"
     CDS             18681..21239
                     /gene="glnD"
                     /locus_tag="ACFM8U_000135"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC6333451.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKAKRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGGIGELCESVRDDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLT"
     gene            21386..22849
                     /gene="guaB"
                     /locus_tag="ACFM8U_000136"
     CDS             21386..22849
                     /gene="guaB"
                     /locus_tag="ACFM8U_000136"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC6333452.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREIILNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(23187..25562)
                     /gene="rnr"
                     /locus_tag="ACFM8U_000137"
     CDS             complement(23187..25562)
                     /gene="rnr"
                     /locus_tag="ACFM8U_000137"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC6333453.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQF
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAIKVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAGKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            25651..25737
                     /locus_tag="ACFM8U_000138"
     tRNA            25651..25737
                     /locus_tag="ACFM8U_000138"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25685..25687,aa:Leu,seq:cag)
     gene            25773..25859
                     /locus_tag="ACFM8U_000139"
     tRNA            25773..25859
                     /locus_tag="ACFM8U_000139"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25807..25809,aa:Leu,seq:cag)
     gene            25898..25988
                     /locus_tag="ACFM8U_000140"
     tRNA            25898..25988
                     /locus_tag="ACFM8U_000140"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25932..25934,aa:Ser,seq:gga)
     gene            complement(26387..28198)
                     /gene="typA"
                     /locus_tag="ACFM8U_000141"
     CDS             complement(26387..28198)
                     /gene="typA"
                     /locus_tag="ACFM8U_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC6333454.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKHTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(28316..29206)
                     /locus_tag="ACFM8U_000142"
     CDS             complement(28316..29206)
                     /locus_tag="ACFM8U_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC6333455.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDTLPFAVIAGFPEIMLDTADSLAKRQD
                     INAWVVGCGLGTGRAAVGTLAGILTEHTDKLVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADIIKESAGIAAGL
                     LAGEIAPAARWLRNRITEST"
     gene            29270..29926
                     /locus_tag="ACFM8U_000143"
     CDS             29270..29926
                     /locus_tag="ACFM8U_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC6333456.1"
                     /translation="MKNKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFVLFLLVFRLCWGIWGSDTARFSRFVQGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAVH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
BASE COUNT         8154 a         7594 c         6748 g         7622 t
ORIGIN      
        1 gggaaagatt tgcaatcgat tccgttgtat agtggtaaag cggccatcgt gttcggcaaa
       61 caactcgttt ttcatacggc ctaaaacttg ccaataggct ttcaatgcgt ccatatcgtg
      121 cgcaggtatg ccgccatcca taaagatagg cggcgagatt ttacaggtct tcgacttctc
      181 cgctgtcgat atagcgaggc agattaaggt tgtaatcttg tgctgcgatt tcgcttaaat
      241 gcaccatacg gctgtaacgg ggaatttctt cctgttttgt gaaggtgtcg atgattttgt
      301 gaatgtcttg ttcgcgcaga cggtttttgt tgccgtcttt aataaagccg cgcgatgcgt
      361 caatcataaa cacgctgccg ccgctgataa cttggtttgt tccctcttcg gcaaattggg
      421 cggtttgggc gtgttctttg tcgatgacga tgatgcaggc aggaatgccc gtgccgtaaa
      481 acaggttggc aggcagcccg ataatgcctt taataaggtc aaggttaagc aattccgtgc
      541 gaatgcgcgc ttcggcattg ccgcgaaaca gcacaccgtg cggaagaata atcgcacctt
      601 tgccgcttgg tttcaggctt ttgagcagat gcagcaaaaa ggcgtaatcg ccgttttttt
      661 cgggcgggat aaggcggatt ggcaacggca aaatcgaagg tcttaagccc gtcgttttca
      721 tcacggaaag acgaatcgga caaggtgttc cggtgttgat ttcggcggtt tcgttgttgt
      781 gcaaaatcat tttcatacgg gcaagggacg cggttgccac atctttttcc tgaccgtaaa
      841 ggctgatttg gctgccggct tgggcggcgg ctttgagcaa cagcgaaccc gagccgcagg
      901 tcgggtcata aacgctggtg ctggacgggc aatctgcgct gattccgata atcttcgaga
      961 cttcggcagg cgtgtaaaac tgccctttgg atttgcctga ctcggtcgca aaatggcgca
     1021 tcaggtattc gtaggcatca cctaacaaat cgtcgtcttc ggcttggttg gaagaaaggt
     1081 tgagcttttc aaaaatgcca acaagcctgc tcaaacggtc gatcatctcc ttagctttac
     1141 ccagtttgtc ttcgtcgttg aagtcggcaa cgtcgatcac gccttttaag tcgttggctt
     1201 cggcaatttg ggcgatgatc ttattcaggc gatcgccgat gtcggcggtg tttttgagtt
     1261 tgacgatgtc gtcaaaagtc gtaccggcgg gcagctcaat catgccgccg ccttgcttgt
     1321 gtttgtcgga aacgtatttt aaaaacaata gcgtaagaac atagtcttta taaaggctcg
     1381 catccatacc gccgcgcaac tcgtcgcagc ccgcccaaag tgatgcataa agctgatttt
     1441 tttttgattg ccataaggtt ttgcctaata aatggaaatt acaaatcaaa cgcgatttta
     1501 acacgccggc ggcaacggct atatcggcat tcacagcttc cagtttatcg gatcgatacc
     1561 gtgccggctc aaatagctgt ttgcctgtga aaaatggcgg cagccgaaaa aaccgcgata
     1621 tgccgacaga ggagacggat gcggtgcggt caatatcaaa tggttttgac tgtctatcag
     1681 tttccttttt tgttgtgcat acccacccca cagcataaag acaagatgct tgcggtgtgt
     1741 cgcaagctgc ctgatgacgg tatcggtaaa gcgttcccag cctaaaaggg cgtgcgaatg
     1801 cgcctgtcct gcacgcaccg tcaaaaccgt gttcagaagt aatacgccct gctccgccca
     1861 cgctgtcagg cagccgtgcg cgggtatgga aaagccttcg atgtcggttt ccaattcttt
     1921 gtagatattg agtaaagacg gcggtatgcg gataccctgc cggacggaaa atgccaaacc
     1981 gtgcgcctgc cctgccccgt gatacggatc ttgtccgaga atgacggctt tgacccggtc
     2041 gaacgctgtc aggcggaatg cgttgaacac atccgccgcc ggcggataga tgatttgtcc
     2101 cgacaaacgt tcctgcctga ctgcatttaa aatttcctga aaatacggct gctgtttttc
     2161 gccgccgagt gcatcgtgcc aagtgtccat atctgcccta tcctttcaaa ttcggctata
     2221 ctgctgatat tgcgttattt tgtcaaacga cgcgctttgt ggggaaatgt atgactgtat
     2281 ggtttgttgc cgctgttgcc gtcttaatca tcgaattatt gacgggaacg gtttatcttt
     2341 tggttgtcag cgcggctttg gcgggttcgg gcattgctta cgggctgacc ggcagcacgc
     2401 ctgccgccgt cttgaccgcc gctctgcttt ccgcgctggg tatttggttc gtacacgcca
     2461 aaaccgccgt tagaaaagtt gaaacggatt catatcagga tttggatgcc ggacaatatg
     2521 tcgaaatcct ccgacacaca ggcggcaacc gttacgaagt tttttatcgc ggtacgcact
     2581 ggcaggcgca aaatacgggg caagaagagc ttgaaccagg aacgcgcgcc ctaatcgtcc
     2641 gcaaggaagg caaccttctt atcatcgcaa aaccttaaca ctcggaggaa ttatggaatt
     2701 tttcattatc ttgttggcag ccgttgccgt tttcggtttc aaatcctttg ttgtcatccc
     2761 acaacaggaa gtccacgttg tcgaaaggct cgggcgtttc catcgcgccc tgacggccgg
     2821 tttgaatatt ttgattccct ttatcgaccg cgttgcctac cgccattcgc tgaaagaaat
     2881 ccctttagac gtacccagcc aggtctgcat cacgcgcgac aatacgcagc tgactgttga
     2941 cggcatcatc tatttccaag taaccgaccc caaactcgcc tcatacggtt cgagcaacta
     3001 catcatggca attacccagc ttgcccaaac gacgctgcgt tccgttatcg ggcgtatgga
     3061 gttggacaaa acgtttgaag aacgcgacga aatcaacagt actgttgttg cggctttgga
     3121 cgaggcggcc ggggcttggg gtgtgaaggt tttgcgttat gagattaaag acttggttcc
     3181 gccgcaagaa atccttcgct caatgcaggc gcaaattact gccgaacgcg aaaaacgcgc
     3241 ccgtatcgcc gaatccgaag gtcgtaaaat cgaacaaatc aaccttgcca gtggtcagcg
     3301 cgaagccgaa atccaacaat ccgaaggcga ggctcaggct gcggtcaatg cgtcaaatgc
     3361 cgagaaaatc gcccgcatca accgcgccaa aggtgaagcg gaatccttgc gccttgttgc
     3421 cgaagccaat gccgaagcca tccgtcaaat tgccgccgcc cttcaaaccc aaggcggcgc
     3481 ggatgcggtc aatctgaaga ttgcggaaca atacgtcgcc gcgttcaaca atcttgccaa
     3541 agaaagcaat acgctgatta tgcccgccaa tgttgccgac atcggcagcc tgatttctgc
     3601 cggtatgaaa attatcgaca gcagcaaaac cgccaaataa gccgtgatga aaatgccgtc
     3661 tgaagctctg ttttcggggt tcagacggca tttttattta aacccgggca aggttgtatt
     3721 ccgcttcaaa tatgtttcgg aaccgcctac ctgccccggt aaaaactgcc ggcaaaatat
     3781 ttctgcacgc cctcgcgaag caagaccaag gttacggcgg ccaaaggcaa tccggccaac
     3841 attccgacaa agcccatcag ctgcccgaac gccatcagcg aaaagataac ccaaaacggc
     3901 gacagcccga tacggtctcc cacgattttc ggcgtaatga aaaaactttc gagaaactgt
     3961 cctacggcaa aaaccgccca aaccgatagg atgccgttcc acgaaccgaa ctggagcaag
     4021 gcggcgacgg ttgccagcag cagtcctgta aacgcgccca aatagggaac aaaaaccaaa
     4081 atgccggcaa gcataccgat ggcaaacccc gaatccagcc cgaccagcac caaccccaag
     4141 ccgtaaacca aacccataat cagcatcacc agaagctgcc cgcgcaaaaa ttcgcccaat
     4201 acctcgttca aattgcctgt aatgcgcgta taagcaccgg caaaacgcct cggaaccagt
     4261 ttggcaatgc cgcacgacca ccgctgccaa tccagcagga aatagtaaag cagcaaggga
     4321 agcagcagca ggttgccgat actgctgaca atattgccgc cctgcctcat caaaacggga
     4381 aaccacgcct taagcgcgtt gctcaactct cccgtatgcg cctgaagcca cgcaataata
     4441 gatgcctgat cgatttccac atatccgccg attgtatttt tcaaccacgg cagcagcgtg
     4501 ttctgcataa aaccgattaa ttggggcagg cgcgatgcca aattgttgaa ctgcccgacc
     4561 agcataggga cgataatcaa caataatgcc aacaacaaaa tcaaggaaaa caccatcaca
     4621 gacatcgaag cggatgcacg gttcaaaccc tttttctgca accattcgac caaagggtcc
     4681 aatacatacg ccagcaccgc cgcaaccgca aacggagtca aagtatcgcc gagcgcgaaa
     4741 accagccaaa ccaaggcggc aaacgccgca ccggcaccca tccacggctt gatgccccgc
     4801 cctttcctcc gatacataaa aaccaccctt ctaaaaataa tattgcgcgc cagtatagca
     4861 gaaccgccgc gccgtcaaaa aagcctccgc cttcccgata gcctgttatc gcggtttagg
     4921 ctaaaataac gcacatccga tgccgtctga aaggcaaacc cgccttcaga cggcataccg
     4981 tacacgataa ggcagcacat catgaccgaa caagaaaaca aaacatccct catcgaattt
     5041 ccctgcacct tcccattgaa agtaatgggc gcggtgcatc ctgagttcga gcaggcagta
     5101 ttggacaccg tccgtctcca cgcccctgac acgcaggctc accacattac cacgcgtccg
     5161 agcagcaaag gcaactatac cggcgccacc gtacaggtaa aggttgaaaa ccaagaacaa
     5221 ttggacaaca tctaccgcgc gctgacttcg cacgaactgg tcaaagtggt actttgagat
     5281 gaaaatcata cataaaggtt tggttgaata tctgccgact tttgaggcaa tgaaaacctt
     5341 taatgccgga cgtaatgccg acaccgaaga cgaactctgg gttgtcgagc atccccccgt
     5401 gttcacacaa ggactggtgg gaaaacccga acacctgctg attcgcgacg acattcccgt
     5461 cgtccaaatc gaccggggcg ggcagattac ctatcacggg cccgggcaat tggtcgttta
     5521 tacgatgatt gatttcaaac ggcgcaaaac cagcgtccgc aacatcgttt ccgcgcttga
     5581 aaacagcatc atcgccacat tggcagaata cggcatcgaa gcggcggcag accccaaacg
     5641 ccccggcgtt tatgtcggag aacgcaaaat cgcctcactg gggctgcgta tcaaaaacgg
     5701 ctccgtctat cacgggcttg cgctcaacgt caatatggat ttaagcccgt ttacccacat
     5761 caacccctgc ggctacgccg gtatggaaat gacgcaaatc gcggattttg tccaaccctg
     5821 ccccacgccg gacgaagtcg ccgccaaact taccgcacac cttgaaacac aattcacacc
     5881 gaaagcagac aacaatgagt gaaatcaaaa ccgacgaccc caaacgcggc atcaaactca
     5941 gaggcgcgga caaaaccgcc cgcatcccca tcaaagtcgt cccccttcag gaaaaattaa
     6001 aaaagcccga atggatacgc gccaaacttc catcgcgcaa attctttgaa atcaaagaca
     6061 ttttgcgcga acaaaaaatg cacaccgttt gcgaggaagc ctcctgcccc aacatcggcg
     6121 aatgcttcag caaaggcacg gcgaccttca tgattatggg cgacatctgc acccgccgct
     6181 gcccgttctg cgacgtggga cacggccgcc cgaatatgct cgaccccgac gaaccgaaaa
     6241 acctcgccga atccgtcaaa gccatgaacc tgcgttacgt cgtcatcacc tccgtcgacc
     6301 gcgacgacct gcgcgacggc ggcgcacagc atttcgccga ctgcatcaaa gccatccgcg
     6361 aaaccagccc gaacaccaaa atcgaaatcc tcgtccccga cttccgagga cgcttggaca
     6421 tcgcactcaa aatccttgcc gaaaccccgc ccgacgtgat gaaccacaac ctagaaaccc
     6481 atccgagcct gtacagaaaa gcccgtcccg gtgccaatta tcaacattct ttagacttat
     6541 taaaacgtta taaagaaatg atgccgcaca tcccgaccaa atccggcatc atggtcggct
     6601 tgggcgaaac agacgaagac gtgcgtgaaa ttatgcgcga tatgcgggcg cacaatatcg
     6661 aaatgattac catcggacag tacctccagc cttcagacgg acacctgccc gtcctgcgct
     6721 acgttacgcc cgagcagttc aaaatctttg aaaaagaagc atacgaactg ggcttcagca
     6781 atgccgccat cggcgcgatg gtacgttcca gctaccacgc cgacgaacag gctgccgaag
     6841 ccttacgcga atgtgaattc tgacgctatt tgagactgaa ctacgctatt tgcgacttta
     6901 taacgctaag ttcgactggg caaatatgga tcaacaacca gttattgaaa ttgccgaatt
     6961 cccacatgat aataaattgt ggcgaattga tgggttaggg aagatttttt cccaattgaa
     7021 aaataacaat aacctgttta ttgaagtcta tttacgccca gttaactttc acgataattc
     7081 tgtatataac tttgctaaat ttgatagttc tggtagagaa attccagaat ataatcaatt
     7141 gaaaaagatt cctgttaata taggcttgct tcctatttta agagtgggat ctctttggat
     7201 aaaaggaaaa cttcaaaaca ataatgcagt tacagatata gccactctta aaaatattga
     7261 aataaatgca aatacagtag aaataattaa tagcaattac aaaataaaat atttagattc
     7321 aagcggaaga gaacaagagt gcaaactatt gttgcagaaa aattttgcaa cggatgcaaa
     7381 agtacctttt tcttgcttca aaattcaaaa tgaaaattac aaaaatggaa taattattcc
     7441 agtaatagaa attatccgtt tttactatgc ctgttcaact agactagctc atttaacatt
     7501 ttctaatcca gacgaaatta caaaaatctc atatgcgata gataatgaaa cagacaaggt
     7561 agtcatcaaa ttaccacagc aatatacaga tcaagaagcc tttattttag caagaatatt
     7621 gacttcaaat attgctttat ctggactctt tcagattaaa aattccctaa taaaaatagg
     7681 agttaatgaa caaccaacta cattattatc taccaatttc ccatttatca atccaaccaa
     7741 tttaatggtt cgcggtatta aaattgttga tagattctta gttacggaga tttgttcttg
     7801 ttcggctcct tttcccgata tttctgttga taggaataat gacaatcgaa agggagataa
     7861 aaaaacagat atacctgatg ataaaaagaa agaatatcga agagggggac aaactcctag
     7921 tctcaaggga aattccacta ttcaaaatca atatgaatca gtcaattgcg tatcaacatt
     7981 acaatttaac ttaaacataa attgcttttc agatttgatc ggtaaaaaga ttgaaaaacc
     8041 ccaaaagccc tttaatgaat acaaagcaca gaaacttgcc ataaatgaaa aaattaatgt
     8101 aactagctta ggagtggatt taggtacatg gagccaaact acaattgtgc ctgcgtccat
     8161 tagcactttt aatacccaat ctgaaaaaca aaatagggat gccttagctg cgacttttga
     8221 caattccgta cgtgctattg aacatttgaa tgaagaatat tcagatattc aggcaactat
     8281 ctacatgtct agctctcgag cttgtgagtt tgttccatta actgactcag cccataaagc
     8341 ccaatggtcg tatcttgttt ccaaaaccaa aacaaggcgt aaggtattat taggaaaaat
     8401 tgagtacaaa gataaaacat tttggttgat tgaaattcaa gctcggaaaa gtgagcattt
     8461 tgttacggcg attatttatt ttgaggcaat tgacgagaca aagtatttga ctcaattatt
     8521 atatgtctta gcaaaacagc ggggaatttg ggataaagtt ccaaaaaact ccagtttgca
     8581 aattattact ctaaagcata cgcataaaga tatcaagagc tacgctcaaa aaatacacaa
     8641 aaaaatatca aattttatct taaaataaat tagttagtaa tataactaat ctagttataa
     8701 attaccaaaa tacttcaggc tgatactttt atataaaatt taagttacct actctcttat
     8761 tgataaggct ggtggtgtcg taactactat ataggagtaa tggttcccat ctgctaacat
     8821 aaatgttaag gtaagagtaa attctaaaga cacaaaagaa aacggttctt ttgatatttc
     8881 aaggttatgt ttttaatcta taacataata ttaacaattt ctatttttag gtattcaaaa
     8941 tgaaaaatcg agatactccg cataaaaaat ttatcaaccc tacggaatat agtctatttg
     9001 cagaagaaga acaatatgat tttgaattaa tgattgaaaa tcagataaaa gaacatgaaa
     9061 aagaaattga ttactatgta actgattatc ccatagaaac actagttgct aaattagatc
     9121 gacaagaatt gtttattcca gactaccaga gaaaaaacat ttgggaaaaa catcgtaaaa
     9181 gtaggttcat tgagtctgtt ttaattggct tacctatccc atttttattt ttttggcagg
     9241 atccttcaac tggaaatatg gaagtggtag atggtgcaca acgactaaca gcattgtctg
     9301 aatttattca tggcaattta aaattatcct atttggaaaa gcttatttat cttaatggcc
     9361 aaacttatca ccaactccta gaaagtcgtc aaaagatttt caaaaataaa cctatccgag
     9421 gcgtagtact tagtaataaa acagacaaag aagctcgact tgacttattt gaacgtctta
     9481 atacaggtag caaaaatgct acacctgcag aaattcgtcg aggtctgctt caaggagcat
     9541 tttacgatat ggttgagact ttatctgaag ataaaacatt taatgaatta gcaaaaatta
     9601 gcaagaatat gcaggatatg ggggagcgag aagaattcat tactcgcttt tttgcctatg
     9661 gtgagtattt agaccattat tctgctaatg ttcgtaaatt tatctttgat tatacagaaa
     9721 tcatgaatga agaatttgct aaaaatccaa acatgcgtgc gacttacgaa caaaaattcc
     9781 atgaaattat ggcttttttc aaagaaaaat atccaattgg ttttaaaaga gatggaaagg
     9841 gtcagtcttc tcgtactcgt ttcgaaggca tggctgtttc agcttatctc gctgaaaagg
     9901 aaaatccagg cattttagct caagttaccc caaaggagtg cgttgatatt tttgacagta
     9961 aagaattcat tgaagtaact ggtagtgatg gtgcaaatgc cactaaaaag ctacatgcta
    10021 gattattttt tgctaaaaat gcctttaaac ggaatcatcc atgaactcag taaaatctat
    10081 gatggaaagt cgtgaagagg aaataaacct atatatggat ctgctaaagt atatccatga
    10141 tagtactcaa catggtttac cagctttaca aaatagaaat ggtgttgaat atcaattgga
    10201 aaatactcat ctccatatcc ttaccgctag tatctatatg ttactatata actacattga
    10261 ggctgttgtt acccaaagcc taaatacttt aatttgctat attcaaaatg aaaggatgcc
    10321 tattacccaa ttttcagaac ccatccaaaa agaatggcta ataggagggt taaaccttaa
    10381 tgatgcaaaa gctagtgtag aaacacgatt aggtaatgga ctcaaaatgc ttaaacattt
    10441 cggtgataca caaaaaatag atattaagat ttctaaagga ggaggaaatt ttaatgatga
    10501 cgaaatattt aagcttgcaa agaaacttca agttaatatg tgtatcgatg acgaaactca
    10561 ccgccaatta cggcaagcag gaaatattac ctatgaaagt acgcgcaaat ttcaagatac
    10621 ttgtatgaaa gaaataaccc gtgaacgaaa taaacttgct catggagaaa tcacctttca
    10681 agaatgtggt agaaaccgaa caatatctga tttaatggca ataaaagaca ctactttccg
    10741 ctatatgcat ttatttactg atgcatttac tgaatatcta atgcagaaga aatatctcaa
    10801 accaacagtt taaaactatt tccgattgcc tcacctagca atacaggaac agcattacca
    10861 atcattcgac taatttggcg aattggagta tcagtttctt gagtgaactg gtaatctaga
    10921 ggaaaacttt ggaataaggc tgcctccctt aaagaaattg cacggtcttg ttccggatgc
    10981 ccaaagcgtc cattgccgaa gccaatacat aaagttgtca ttgttggggc gggcttgtcc
    11041 cattccatac gaccataaat tcgtttataa gactgtcctt tttttttgcg atgacatgcg
    11101 gctcgtaaat cttctggcca atccatccat gttcctcctg gacgagaagc acgaatccgt
    11161 tctagattac gagctgacat tgatgaacac cgatgtaata catctatagt atcttgtttg
    11221 cctgctgcca gtggcggcaa gtgtccaata gcctgacgaa ctgttacatg gcaatttgaa
    11281 tgcgttggag gaaccatctg aatattaccg atacgtgatg caagtaatac gtgtctacgt
    11341 cgtgattgag ggactccata atctgcacat aatacttttt cagcccaaac ttggtatccc
    11401 tgttgtttta aattatttac aaaatcttgg tatacctcat gttttacaac atctggcaca
    11461 ttctccatag tcacaagctc tggttgcact tcatgaatca agcgagaaaa agcgtatagt
    11521 aatggccatt ttttgtcatt acgaacatca cgaccttgat tgtaagtaga aaatggctga
    11581 caaggagcac aaccagctaa taatctaact gcatcttttg aatagtgcgc ctgaatctga
    11641 tctccagtaa ctgtttccac atcttgtaat ataaattttg cacctttatt gttatactcg
    11701 tacgcatatg cacaagctgc atcaatatca tatccagctt tcactttaat tcctgctcga
    11761 agtaaacctg ctgtcaaccc acctgctcca caaaataaat ctactgcttc aatgttcatt
    11821 tttcaactca tatttttgat ataaggatta tatccttaac tttgattgga gtcgagcttt
    11881 taatagtata taagctctga atagtacagt tccttatgaa cgtttctatt catttttaaa
    11941 attatttcct gaaaactttg ggggcaatca ttaaaatcct tcaataaatg gttataacgg
    12001 acggctttat cttgccatgc tttaaataga ttttttggaa aattttgtat taaccacaaa
    12061 caagcatgaa tagtactgtg tcgttccgcc aaactgcgac tttcccaact cagtcgacga
    12121 gaacgttcta attgaactgg aatctgatgt atttgcttgc acaaataaga gtacaaatcc
    12181 ggtgcaagct tctttgatgt agtaatcgcc aaaaagtgat gcagaatttt tagttgttct
    12241 ttttcagatt ggttaaaaag ataatcaaga ttagcaattt ggtttaacca attttgccat
    12301 tgcagcctga tttcatcatt aaagaaaata acattctcac aaggactgtt gctcaagtca
    12361 tattgacaat tccaacaata tttcaactct gtaaagcgct gcaaattagg tttacctaaa
    12421 tcttgtcggt gaaaagcaat atatatgcca catttaggac agcaatcatg catcatgatt
    12481 tgatgtattg gacaaaatgt atatagggct atgcgccatg attttcggaa atatgtttca
    12541 tggtcagtag ctagacatag cgggcaataa acaatagcat tattatcgcg tttacggtga
    12601 ttggctttaa ggctgtttat ccacgtcaat tgtcctgaaa aattgttttt atcaaataat
    12661 cgaccttgta ggctatctaa tgtggttgtt ttgattcgaa tggttgaaca gcctgttttt
    12721 tcagccaatg tattgaccaa ccagtcagga gtatggcgat ctatatccct attccatatc
    12781 tcataattag gaaatagcaa atgacagaag gtttgtactt tcaacccgtt tgcatgtgct
    12841 aagcgaacca accatgatgt aaacaattca tcaggataag gtttcggatg agcgggtaaa
    12901 atcatctgag tttatccaat tgacgtttac gttggcttgg actaatccag ttgattgaat
    12961 ttaagattgt ttcgttaata cattcagttt gttgttgaat ggcggctaca gcagcttgtg
    13021 tcagcaaacg agaaagctct ccaatatatc cttcactcat gccatataac tttgtagcca
    13081 acgaaatttc atgtaaatcc gacggctgac taagcggcag catccgttca aagctcatca
    13141 ataaacgcat aaaatcctta tttaattccc aacgaggaag caccgcaggt tcaaaacggt
    13201 tggccaactg tgcatcagtt tgaatggctc gatatgcgtc tttaatacct acacctacaa
    13261 tcggaatttg taattcattt cctaagtatt taattacatt taaaaacgtt ttctgcttat
    13321 tcaaactccc ggaaaggata tggtggattt catcaataat caacatttta gtattaacgt
    13381 atttgagcaa atgaagcact tgggcaagtt ttttatctgt cctgtcatga gttttatagg
    13441 gggcaaacag taaatctaaa atagcattgt aaaaacggct ttcatcaggt ataggcggtg
    13501 cttgaataat cactactggt gcgataatac catttccatg tggattatca tgggctggat
    13561 gttgccgcaa aaaatgttta atcaacatgg ttttaccgtt attagtatca ccaaccaaca
    13621 acatattagg catacggttt tgtttcggga aacttagcaa atcttccagt tttaccaaaa
    13681 cctgttgagc ctgcggataa cctatccaac gaggggaacg tatgtattcg atgcgctcaa
    13741 tatcgctata ctttagttgt tcttttgctt tgggagaaag gtgaggataa ttcatgataa
    13801 atcgtccaaa tcatcaaaag gctgaatatc atcaaacata ttttcctcat tttcagacga
    13861 tataatattc ctcgaatcaa tttgaggcat aggtaaagat gctcgagtac gttgatctaa
    13921 ttggcgacgt tgttgagcgc gacggatagc tttagtctta ccttgagctt gctgttccaa
    13981 ttctcgcatt cgtccgtatg ctgtaaaaat catgttctca tcaatattct gtttctgctc
    14041 attttttaat tgtctctgaa tctctcgtaa ctcccaaatg ctgataactg gattcgaact
    14101 gttccgataa ggtattggaa aatacatcga taaatctggg tcataaaacc aaatcacgct
    14161 taaatcacgt ggatctatgc gaaaaatgaa ctggcgagcc tgtctaggat tagtcggatt
    14221 agttgctgca atccatggtc gtaaaacgtc atgccaataa gtgattttat ggataactac
    14281 cccatattgc tgcaccgttc gcatttcaaa cggcataaag gtcaagcgca atagtgtctc
    14341 atcaaccact ttatcaggta atcctgttcc taaagaaaca tcatctccta atacacctga
    14401 tttccatttg gctaacggtg agatccccaa tccagaatga gtacgctgat gataaacatc
    14461 acaaataaat agtgttaacc acgtttcaaa ttcagttagc gttaatgccg ccttgctttc
    14521 ggaagcataa tcttttctct gttgtgtatt tgcaaaagta gtaccagtta aaccgtggac
    14581 ttcatccaaa atcgtaccta gtaaacgctc aatgtgtcct ccgaaatgag gacgtgccac
    14641 aggcctccac tcaatttcaa taccgtattg tttacacgcc ttttccagca ctttgccacg
    14701 gaattctttt gcattatcca aatgaatttt gcgtggcaaa ccccacacag gccaccgccc
    14761 tttcactcca tgttttgcca accagctttc cttagacaaa atactatgtg ctaaacacaa
    14821 acctgtagat aatgcacttg gtggatcgaa tgacacataa aatcccgtaa ccattcgact
    14881 gaacacatca atcgccagcg taatccacgg cctaccaata ggcttacgat atatatcgtc
    14941 aactaaaata atgtctaact ttgtatgatc tatttgtacc actgaaagcg gataatcggc
    15001 atttggaaag ctaccttgta aaggagaaaa tttcatttct gctgctttat ggcttagtcg
    15061 tttagcaatt ttaatttgag gcgcaatatt tttgattcga gaacgaatgg tattagcatg
    15121 aggagctggt aaattttgtt tatggcaacg ccgtgtaatt tctctaatga ttttctgtat
    15181 tgatttacgt tgtgtgctta aataatccac ttccaatact tgcctgataa gctgttctac
    15241 atcatcagaa agctgtgttt taccactatc actacgctgc ttacgtgtta aagcagtcag
    15301 aacgccgctt tccttataac tttttaacca gcgataaacc gtactgatat gtacaccgca
    15361 ttcggcagct ctcgcttgaa catcagattt actacgtttt ggatttcgga taagtggctc
    15421 aattacatag aaatgttgtt gagctaattg ccatttttgc tcaggaatac tatctaagtg
    15481 ggttaattca gcactctgga ctgttgatag atccgctaat tttgcttggt aatctgcacc
    15541 ggttacacta tcgcgtaact gatatgtttc taactccagg actgctgtaa ttacacagat
    15601 tttgttttga aacaaaacca ttgcaccgac attcacattt aaagttgctt tcatttttta
    15661 aaccatatag cggattgcat agtgagtggt tgatttaaat ctgcgccaat ttttcgttct
    15721 gcaattaagt accacaatac aggcaataat tttgcttgat tgtattcatc ctgaaaagtc
    15781 tgttgaataa gctgtttagg tgtggatttt ttcaattcgc gtaacgattc atccaataaa
    15841 gtcatatatg cttcgctgta ttcgccagat aagccatttt gttgatatgg caataaaaag
    15901 cgtacattgc ataaataatc agtatgaatc tctaactctg taatcagttt aaaatgccaa
    15961 ccgtgtaatt tgcaaaaatg taaggcagcc tgaaatttag gtttgagttc acaccagttt
    16021 ttacgtaaat cgctacgata ttttacttcg taaataatta catcattttg ataatgaacc
    16081 aaaacatcag gggtatagat atgcttcttt cctgctgggt caaaccactc aatcgtgata
    16141 ggctgtactt cgaaatgctg cacattagaa tcaaattcca gcagagttaa aaaatcacgc
    16201 tccaaagatg actcataagc tgcaatgcct atagctttgt tatatggagc gatgcctgta
    16261 atatttcgat agtttttagg aattttacgg attggcatag ttttatatgt gtagtagaaa
    16321 aaggattaaa aggaagacaa tcttaatgag ttaatgccct ttatcaataa cccaaactca
    16381 attagctttc ttataattta gtctcaaata acaatattat tttcaaatct taatgcaaaa
    16441 tttttgctct cagtcgcaaa taacgttatt agaagtataa gattttgcat attctagtcg
    16501 caaacaacgt tggaattcac agcgaaagcc acggcggttg cgggcatcat taaacccgga
    16561 acaccgcgcc aaagtgaaga acgattccct gcgcctgccg cgccgtttga tgtaaacaac
    16621 atcaatttga attgtccatg ccgtctgaaa gcccttcaga cggcatgatt tattcccaaa
    16681 atccgataat ctcaaaagaa tctgtcagtt aatgagaacg attcattata aattttgata
    16741 aaaaaccgtg ctctatttgt tttttgctat ggttaataag tgtaaatgtt ctttattgat
    16801 gagatggatt cttagtttta gcagcaaaaa aaaacgataa gaaacagagc attgatttgg
    16861 aaggcatcgg ggaatacgcc ctatttcttt tgataattgt ttgtatttta aatagaaaat
    16921 ttgtcatgcc gacagctttt gcatacaatg cacgcatccc gaacagtgct tcatccactg
    16981 tttaaccaca cgaaacccaa attaagaaaa gagaaatatt atgcaaggca atcaagcagt
    17041 tttagattac atgaacgaat tgctgtccgg tgagctggca gcacgcgacc aatacttcat
    17101 ccactcccgt ctctactccg aatggggcta caccaaactt tttgaacgtc tcaatcacga
    17161 gatggaagaa gaaaccacac acgccgaaga ctttatccgc cgcatcctga tgctgggcgg
    17221 tacgccgaaa atggtacgcg ccgaactgaa tatcggcacg gacgtggttt cctgcctcaa
    17281 agccgacctg caaaccgaat atgaagtacg tgatgcgttg aaaaaaggca tcaaactgtg
    17341 cgaagaggct caagactatg ttacgcgcga cctgatggtt gcccaactga aagataccga
    17401 agaagaccac gcacactggt tggaacagca gctgcgcctg atcgagctga ttggcgaagg
    17461 caactactac caaagccaac tgtaagctgt attcggaata aaaggagtcc gtatgaaagg
    17521 cgaccgtctg gttatccgcg agctaaacaa aaacttaggc ttgctgctga taaccatcaa
    17581 ccaatatttc cttcacgccc gtattttgaa aaactggggc tttaaagaac tgggcgaaca
    17641 tttcttcaaa cagtccatcg ttgaaatgaa agctgcagac gatttgatcg agcgcattct
    17701 gttcctcgaa ggcctgccga acctgcaaga actgggcaaa ctgttgattg gtgaatctac
    17761 cgaagaaatc attgcctgcg atttgaccaa agaacaggaa aaacacgaag ccctgcttgc
    17821 tgccatcgct acagcagaag cgcaacagga ttacgtcagc cgtgatttgt tggaaaaaca
    17881 aaaagacacc aacgaagaac acatcgactg gcttgaaacc cagcaggaac tgatcggcaa
    17941 aattggtttg ccgaactacc tgcaaacagc ggcgcaagag gactaaaaca caaaccactg
    18001 ccaaatatag cagtcccctg catcatcaga cggcatatcc gtataaacgg gtatgccgtt
    18061 tttctttgcc ggtttgcgaa tcaaatctaa attgatttga atcaatactc gataacggtt
    18121 tttgtttaat aataccgata tgaaatctag attgcttgtt tgctgtttcc caatctgtct
    18181 tgattttacc tctttcctct tgatgtgtgt gtgtgtttgg gtgtggctgc cgccacccct
    18241 tttttttggc ttttatgtga agtaaaatcc gtaacagcaa atgcttaact ttaacattca
    18301 tccggaagca aatgatgggc aacaaattga ctctgcctgc cgaactgcct gccgaacagg
    18361 atttgcgggc ggtattggca tacaatatgc ggcttttccg cgtgaacaag ggttggtcgc
    18421 aagaagaatt ggcgcggcaa tgcggtttgg acaggactta tgtgtcggca gtcgaacgca
    18481 aacgctggaa catcgccctg tcgaatatcg aaaaaatggc ggcggctttg ggcgtggcgg
    18541 cgtatcagtt gctgctgccg ccgcaggaac ggttgaacct gatgaccaat tccgccgata
    18601 cccgacaaat gccgtctgaa agcggtattt gaccgccgaa ccatccgtct ccttttttcc
    18661 cgccaatcga aagaataaaa atgcctgcaa acctgtcttc agcactggaa accttcaaac
    18721 agcagcgcga tgccgccgaa gcgcattatt tgaaagccaa gcgcgtgtcg gtatttttca
    18781 gagaatacac ggcggcagtc gaaaccttac tggcggcatt gtgggtagaa cattttcaaa
    18841 acagcgcgtt atgcctgatg gcggtgggcg gcttcgggcg cggcgaactg tatccctgtt
    18901 cggatgtgga tttggcggtt gtctcccctg ccccgctttc agacggcatt caggaacaga
    18961 ttgcccggtt tgttcaaacc ctatgggact gcaaactgat gccgtctgta aaaagcggcg
    19021 gcattggcga actgtgcgaa agcgtgcgcg acgatattac gggggacacg gcgtttttag
    19081 aggctaggtt tttgtttggc aaccgccaaa cggcggataa actagcggaa aaaatgaacg
    19141 cgcagcgcaa tgtggcggcg tttgtcgagg caaaactggt ggagatggaa caccgccacg
    19201 ccaaatcgca aggctcaggg gcggtattgg aaccaaacat caaaagctgt ccgggcggtc
    19261 tgcgcgacat ccacaccctg ctttggatag cgaaggcgca aggcttggcg accgacctgc
    19321 ccgatctgct caaacagcgg attttgacgc gtgccgaagc cggtatgctt tcgcacggct
    19381 accgccgcct cgcccacatc cgcatccatc tgcatttaaa cgccaatcgc gccgaagacc
    19441 gcctgctgtt cgatttgcag ccgcaagtcg ccgaaagcat gggttatgaa ggcttgaacc
    19501 tccgccgcca aagcgaagaa ctgatgcgcg tgttttaccg cgcgattaaa accgtcaaac
    19561 aactgggcgg catcctcacg cctatgctgc aaagccgcgt ttcctccacc ccgttgcgcg
    19621 ttaccctgcg gattgacgac gactacatcc aagtcaacaa ccaaatcgcc gcgcggcaca
    19681 ccgatatttt tttcagacgg cccgaacaca ttttcaaaat cgtcgaaatc atgcagcagc
    19741 gcaacgatat taccgcgctc gaaccgcaaa ccctgcgcgc atggtggggc gcgacgcgca
    19801 aaatcaaccg cagcttctac caaaattctg aaaaccgcca ccgcttcgcc ggctttttcc
    19861 gcaacggcaa cggactgacc caaaccctgc gcttcctcaa cctctacggc gtattgggac
    19921 gctacctgcc cgcgtgggaa aaaatcgtcg gcctgctcca gcacgacctg ttccacattt
    19981 atcccgtgga cgaccacatc ctcgccgtcg tccgcaacgt ccgccgcctt gccttggata
    20041 tgcacagcca cgagctgccc tatgcctccg cgctgatgca gtcctttgaa aaacaagaca
    20101 tcctctatct cgccgccttc ttccacgaca tcgccaaagg acgcggcggc gaccacgcca
    20161 tacaaggcat cgcagacgcg cgccaatttg ccgctgacca cttcctgacc ggagaagaaa
    20221 gcgacctgct cgcctggctg gtcgaaaacc acctgctgat gtctgccgtc gcccaaaaag
    20281 aagacatcca agaccccgac gtactcgatg ctttctgcaa acgcgtccaa acccacgaac
    20341 gcctcagcgc gctctacctt ctgaccattt ccgacatacg cggcaccaat cccaagctgt
    20401 ggaacgcatg gcgcgccagc ctgctggaaa gcctcttcca tgccgccgga cgctacctta
    20461 caggcaacgg cggcaacccg cacaccctct tcggccgccg ccggcaggaa gccgccgact
    20521 tactcacccg cgccgccgtc cccgaaaaac agcagaaaaa actatggaac gcgctcggtt
    20581 ccgcctactt cgcccgccac cagtcccgcg aaatcctgtg gcacgccgcc aacctagtcc
    20641 acgactttga aacccccatc gtccgcagcc gcatcctgcc caaaagcgac agctttcaag
    20701 tcatggtttt catgcccaac ggcccgcgcc tgttcgcccg cctctgccgc atcttcagcc
    20761 gccacggctt cgatatcctc gccgcccgcg cctttattac cgaacacgac tacatcctcg
    20821 acaccttcat cgtgcaaatc ccctcgcagc acgctcccga agactacccc gacatccaaa
    20881 gcgcgctcga agccgaactc aacagcttta tccacggaca caccgttgcc gaaatctcca
    20941 gccatagccg ccgcatcagc cgccgcagcc gctacatgcc gatcgcgcca agcatcacca
    21001 tcacccccga agaagactat ccggactggt actccgtcga aattaccgcc gtcaaccgcc
    21061 ccttcctgct cgccgatatg gcggaagtct ttttcgccca caacgtcagc ctgcgctatg
    21121 ccaaaatctc cacactggat gaacgcgccg aagacagttt taccgttttt agccccgacc
    21181 tgaaaaaccc aaaaatccag tcctcattga agcaggcttt gctggaacag ttgacataag
    21241 caccctgccg gttttcagac ggtatcccgc cgccgcgccg tacgctgtct gaaggcgggg
    21301 gatattgcaa acaatataaa ctgtcaagcc gccggtaacg tgctataatg ccgtgcatct
    21361 tgcacaaact gaaagaccga gcattatgcg tatcgtagaa aaagcctata ctttcgacga
    21421 tgttttgttg gttcccgcac attcgaccgt gctgccgcga gacgttaaac ttcaaaccaa
    21481 gctcacccgc gaaatcatac tcaacctccc cctgctttcc gccgcgatgg acactgttac
    21541 cgaggcgcgc ctcgccattt cgatggcaca ggaaggcggc atcggcatca tccataaaaa
    21601 tatgccgccc gaaatgcagg cgcgcgccgt ttccaaagtg aaacgccacg aaagcggcgt
    21661 ggtcaaagac cccgtaaccg ttgcgccgac aacgctcatc cgcgaagtct tggaaatgcg
    21721 tgcgcagcgc aaacgcaaaa tgtccggcct gcccgtcgtt gaaaacggca aagtcgtcgg
    21781 catcgtaacc aaccgcgacc tgcgttttga aaaccgcgtc gatttgcccg tttccgccat
    21841 tatgaccccg cgcgaacgtc tggttaccgt ccccgaaggc acaagcatag acgaagcgcg
    21901 cgaactgatg cacacgcaca aagtcgagcg cgttttggtt ctgaacgaaa aagacgaact
    21961 caaaggtctg attaccgtca aagatatttt aaaaaccacc gagtttccca acgccaacaa
    22021 agactccgaa ggccgtctgc gcgtcggtgc ggcagtcggc accggcggcg ataccgaaga
    22081 gcgcgtcaaa gccttggttg aggccggcgt ggacgtgatt gtcgtcgata ccgcccacgg
    22141 gcacagccaa ggcgtgattg atcgcgttcg ctgggtcaaa gaaacctatc cgcacatcca
    22201 agtcatcggc ggcaacatcg ccactgccaa agccgcattg gatttggtcg ccgccggcgc
    22261 ggatgccgtc aaagtcggta tcggtccggg atcgatttgc accacccgta tcgtggcagg
    22321 cgtgggcgtg ccgcaactga ccgccattca caacgttgcc gaagccctca aaggcacggg
    22381 cgttccgctg attgccgacg gcggcatccg cttctccggc gacatcgcca aagccctcgc
    22441 cgcaggcgcg tacagcgtca tgctcggcgg tatgtttgca ggcacggaag aagcgccggg
    22501 cgaaatcgaa ctctaccaag gccgctcata caaatcctat cgcggtatgg gttccttggg
    22561 cgcgatgagc caaggttctg ccgaccgcta cttccaagac aaaaccgaca gcaccgacaa
    22621 atacgtcccc gaaggcatcg aaggccgcgt gccttacaaa ggcccgatcg tgaatatcat
    22681 ccaccaactg accggcggtc tgcgttccag tatgggttac ttgggttgcg ccaatattgc
    22741 cgaaatgcac gaaaaagccg aatttgtgga aatcacttcc gcaggtatga gcgaatcgca
    22801 cgttcacgac gttcaaatca ctaaagaagc accgaactac caccgctgat ttgaacagcc
    22861 tttcaggaca aaatgccgtc tgaagccctg ttttcaggtt tcagacggca ttttttgtcc
    22921 gttcaaggta agccgcacgg gctgattgcg cggacgggca aagcaattcg gacggacacg
    22981 ccgaaagaat gaaaatgccg tctgaacgcc gtttgcgccc cgtctgccga ataccgccgg
    23041 cctttaaaac ggcggtggaa ataaacgcaa tctttcaata cgggcgtttc gattggcctg
    23101 aaatgatgcg ccggccgcca tatcaaaaaa tcgggcggcg gcttcgggaa agtatgcccc
    23161 gccctcccca agccaccccg tcagcattat gatttgcctt tccgtttttt taccttaatc
    23221 ggaaccggtt tctttgcctt tttccccgat tctgcggcgg cagacgcgcc ccttcccctt
    23281 actttgccgc ctcgggctgt ttttttcccg gcggcggttt tcggcttccc tttccccgcc
    23341 gtccctgccg gtttggcaga cgcggatgat ttaactttcc gccctctgcc gctcccctcg
    23401 gcaatcagga caaaatcgat ttttccatca tccaaatcgg cacgggccac tttaatggca
    23461 accctgtcgc ccatattgaa acggatgcca ctgcgttcac cttcgattgc catgatttcg
    23521 gggcggaagt tgaaatagtc ttcgcccaaa tcgctgatat gcaccagacc gtcgatatgg
    23581 atatcgtcca aagtgacaaa tattccaaaa tttgccaccc cggaaatttt gccttcaaat
    23641 atttcgccga ctttgtcacg catatagtag gttttcagcc agttttccac gtcgcggctg
    23701 gcgtcgtcgg cacggcgctc acagaacgag gtatgcacgc ccaaagcctg ccagcttttt
    23761 tttggcgtgt aggtttgctg attcaacacg gctttgatgg cgcggtgtac ggtcaggtcg
    23821 ggatagcggc ggatgggcga ggtgaagtgg gcgtatgctt cgtaggcaag accaaagtgt
    23881 ccgtcgcaat gcggttcgta aaccgcctgt tgcatggagc gcaacatcat gacttgcagc
    23941 aattcggcat ccggcctgcc tttgaactgt ccggcaagcg cggcatagtc tttcggcgac
    24001 gggttgtcgc cgccgccaag ttgaagcccc aacagaccga actgctcgcg caaggcggcg
    24061 agtttttcgg gcgtgggccc caaatggttg cggaacaatg cggtatgctt gtttttcaac
    24121 agaaaatccg ctgcgcaaac gtttgccgcc aacatacatt cttcaatcag cttgtgggca
    24181 tcgttgcgga caacggggac gattttttca attttaccgt tgtcgtcgaa aagcatttgg
    24241 gtttcgatgc tgtcaaactc caccgccccg cgttcgaaac gctttttctg aaggattttg
    24301 aagagtttgt aaagcgtgtc gatttgggct ttgaacggat gcccgatgcc gcctgaaagc
    24361 catttccaaa cttggttgta ggtcaggcgg gcatgagagc gcatcacggc ggggtagaag
    24421 cggtattctt taatattgcc cgcgtaagtg ataaccatat cgcacaccat acacaaacgc
    24481 tcgacattag gattgagcga gcagatgccg ttggacaggt tttccggcaa catgggaatc
    24541 acgcggcgcg ggaagtaaac actggtgctg cgttcctgag cgtccgtgtc gatagcgtca
    24601 tcggggcgga cataatggct gacatcggca atcgccacga ccagacggta attgcgtccg
    24661 attttttcgg caaacaccgc atcgtcaaaa tctcgagccg tttcgccgtc aatcgttacc
    24721 aaaggcaggt cgcgcaaatc gacgcggcct ttcaaatcgc ttttgcgtac atggtcggga
    24781 atttttttca cggctttggc acacgcttca ctgaattggt gcggcaaatg atgcttgcgc
    24841 acggcaattt caatctccat gccgctgtcg gcataatcgc ccaaaacttc gatgattttt
    24901 gccactgccg gccggttttg ctcaggataa acctcaattt cgccgacgat gacctgaccg
    24961 gattcaggtt tgaaacgcgc cacgccgtcc ggttccaata cgatgctttg gttcagacgc
    25021 ttgtcttccg gctccaaaat cgccacgccc ctatccatat agaaacggcc gaccactttg
    25081 ctttgcgcgc gttcgacaat atccagaact gtcccttcgc ggcggcccct acggtccatg
    25141 ccggcaggac gaacagtgac aatatcgccg tgcataatgc cgcgcatctg gcgttcgtac
    25201 aaaacaaaat caccgtcttt ggcgggcgtg agcggcacgg cgaaaccgaa gccgtctttg
    25261 tgcgcctcga cacggcattt gaccaaatcc aatttgtccg ccgcgcaaac cgcgccccgg
    25321 cggttgatta aaacctgacc gtcccgcgcc atcgccttca gacggcgttc gaaaaagaca
    25381 tactcgtctt ccgtaatcga cagctcgcgc gcaagcgatt cgatttttga aggcacacct
    25441 ttgcgctcca acaattcgat tatccattcc cgactgggca aaggatgttc ataacgctgt
    25501 ttttcacgac ttaaaaacgg gtctttttcc cgtaaattta aagatttaat atttttattc
    25561 atttggactg ttgacattct ctttatgaac tatataatag cgacttcttt gcggcaggac
    25621 actgtaccgc aaaacagcaa ataaagcaaa gcccgggtgg cggaattggt agacgcgcta
    25681 gcttcaggtg ctagtatcct cacgggtgtg gaagttcgag tcttctcccg ggcaccaaat
    25741 caaatgcttt gctttatttc aatatttaga ttgcccgggt ggcggaattg gtagacgcgc
    25801 cagcttcagg tgctggtatc ctcacgggtg tggaagttcg agtcttctcc cgggcaccaa
    25861 ataataaatt atcccgattg ggataatttc cgccattaga gaggtggatg agtggtttaa
    25921 gtcgcacgcc tggaaagcgt gtatacgtga atagcgtatc gagggttcga atcccttcct
    25981 ctctgccaaa tacaaatccc aaacttttac agtttgggat tttttgttat tcaaacaata
    26041 atttcattgg gaaaacctgt tattgtctat aatcgtcatc aaccgtttac aactatccgt
    26101 taccgttttt ttatggtacg ttttatcctc ttcctcagcc tgtaaggact caaaaggcgt
    26161 atcccttcaa acggcattat tcgacatgct gccgggaagc cgaacccgat accgaaacgc
    26221 gacggggcga tatgccgctg gaaaaatggc ggcagtttct tgattgggta ggaaaaacgc
    26281 cgtctgaaac agcttcaaga agccgcaaag cccgtccgaa taaaaaatgc cgtctgaaat
    26341 ttttcagacg gcatcgcgta tcatttaaac attaatccag taaacatcaa tccagctttt
    26401 taaagtggcg gcggcgttcc aattcgctca agtaacgctt gcgcagacgg atggattgcg
    26461 gcgtgatttc aacgagttcg tcatcgtcga taaactcaac cgcaccttcc agcgtcagct
    26521 taatcggcgt ggtcaggcga acggcttcgt cggtaccgct ggcacggatg ttggtaagtt
    26581 ttttgccttt gagcgggttg accaccaaat cgttgtcgcg actgtggatg ccgataatca
    26641 taccttcgta gattttgtcg ttgggcgata cgaacatacg gccgcggtct tccagattcc
    26701 acaaggcgta agcgaccgcc tcgccctgct cttgggacac cagcacgccg ttgtggcggc
    26761 cgggcatatc gggtttgacg ggcgcgtaat cgtcgaacac gtggctcatc agtccgacac
    26821 cgcgcgtcaa ggtcatgaat tcgccttgga aaccgatcaa gccgcgcgct ggaatatggt
    26881 attcgaggcg ggtgcgtccg ttgccgtcgc tttccatatt agtcagttcg ccacggcggc
    26941 ggccgagttc ttccattacc gcgccttggt tgtcgtcggg tacgtccacg gtcaggtttt
    27001 cataaggttc gcatttttga ccgtcgatgt cgcggtacac gacgcgcggt ttgccgacgg
    27061 cgagttcgta gccttcgcgg cgcatatttt ccaacaaaat ggtcaggtgc agctcgccgc
    27121 gtccggatac gcggaacacg tcggcatcgg cggtgtcttc cacgcgcagg gcgacgttgg
    27181 tcagcaattc tttttgcagg cggtcgcgga tttggcggct ggttacgaat ttgccttccg
    27241 tacccgccag cgggctggtg ttgaccataa agtccatcgt cagcgtcggt tcgtccacgc
    27301 tcaacatcgg caggcctttg gggttgtctt tgtcggtgat ggttacgccg atgccgatgt
    27361 cttcgatacc ggaaataatc acgatgtcgc cggcttcggc ttcttcaagc ggtacgcgtt
    27421 ccaagccttt gaaacccaaa agctggttga tgcggccttg ggcgatttgc tgctcgtggt
    27481 tcatgacggc aacggtttgg ccgggtttga tgcgtccgtt caagatacga ccgatgccga
    27541 ggcggccggt gtagttgtcg tagtcgagtt gggaaatttg cagttgcagc ggctcgtccg
    27601 cgctgccgct cggtgcgggc gtgtgtttta agatggtgtc gaacagcggg cgcatatcgc
    27661 tgctctcgtc ggtttcttcc agcttggcaa agccgctcaa acctgaagcg taaacaatcg
    27721 ggaaatccaa ctgctcgtcg gtcgcgccca agttgtcgaa cagctcgaaa gtttggtcga
    27781 taacccagct cggacgtgca gacggcttgt cgattttatt gatgacgaca atcggtttca
    27841 gccccaaagc caaggctttt ttggtcacga aacgggtttg cggcatcggg ccttcctgcg
    27901 cgtccaccaa caagacgacg cagtccacca tccccaaaac gcgctctact tcgccgccga
    27961 agtcggcgtg tcccggcgta tcgacgatat tgatgtggta gccttcgtaa tcgatggcgg
    28021 tgtttttggc gaggatggtg atgccgcgtt ctttttcgag gtcgttgctg tccatcacgc
    28081 gctcgtcaac ctgctggttg gcgcggaatg tgccggattg gcgcagcagt tggtcgacca
    28141 atgtggtttt gccgtggtcg acgtgggcga tgatggcgat gttgcggatt tgtttcataa
    28201 ttaatgtttt tcaaaaactg taagagataa ctgcgcatta taccacgctt taccggacag
    28261 tatttgggaa atgaagatgc cgtctgaagc ccttgccttt cagacggcat cttcattacg
    28321 tactttcagt tatccggtta cgcagccacc ttgccgccgg ggcgatttcc cctgccaaca
    28381 gccctgccgc aatgcctgcc gattctttta tgatatccgc cgccgcgccg tgcagccacg
    28441 cgcccgcgca ggcggcttca aaaaccggca cgccctgtgc gagcagactg ccgatgatgc
    28501 cgcccaatac gtcgccactg cccgccgttg ccaatcccgc gttgccactt tcgttgacat
    28561 agatttccgt atcggacgcg gcaaccaatg ttttgtgccc ctttaaaacc acggttgcgc
    28621 cgaaaattgc ccctatcttc ctcactgccg ccgtccgatc cgcctgaacc tgtgcaaccg
    28681 tcgttccaag caggcgcgcg gcttcggcgg ggtgtggcgt taaaatcagg tttttacacc
    28741 cgcgtgccag atttcgggtt tcggcatcgg ttgataatat gttcagcgcg tccgcatcca
    28801 aaaggacgag cttgtccgtg tgttccgtca aaattccggc aagcgttccg accgccgccc
    28861 tacctgtacc caatccacaa ccgacaaccc aggcgtttat atcttgacgt ttggccaaac
    28921 tgtccgccgt atccagcata atctcgggaa aaccggcaat aacggcaaaa ggtagcgtat
    28981 cctgattgaa acccgcccac actttgccgc agccgagata cattgccgcc gatgccgcca
    29041 atacgggcgc gccgctcatc cctgccgatc cgccgactac ggcgagcgtg ccgaaagtcc
    29101 ctttgtgcga atcttccgca cgggctttga aaacatgggg aaaacgcaat gccgtctgaa
    29161 gcatccggcg gcggctctcg cctgaaagat gaaaaacggg aaacattgcc cctccaatcg
    29221 gttaaaataa ggctcgaaaa caaaccgatt ctaccgccgg aaaccgatta tgaaaaacaa
    29281 aaccaaagtc tgggacctcc ccacccgcct tttccactgg ctgcttgccg cgtccctgcc
    29341 ctttatgtgg tatagcgcga aagccggcgg cgatatgctg caatggcaca cgcgcgtcgg
    29401 gctgttcgtc cttttcctgc tcgtatttcg cctctgctgg ggcatttggg gcagcgatac
    29461 cgcccgtttt tcccgtttcg tccaaggctg ggcaggcata cgcggctatc tgaaaaacgg
    29521 tattcccgaa cacatccagc ccggacacaa ccccttgggc gcactgatgg tcgtcgcgct
    29581 tctggccgcc gtgtcgttcc aagtcggcac cgggcttttt gccgccgatg aaaacacctt
    29641 cagcaccaac ggctacctca accatttggt ttccgaacat acgggcagcc ttatgcggaa
    29701 aatccacctc aactttttca agctgctcgc cgttttttcc gcagtccaca tcgctgctgt
    29761 cgccgcatac cgcgtgttca aaaagaaaaa cctcgtcctc ccgatgataa ccggcttcaa
    29821 atacatcgaa ggcaaaacct caatccgctt tgccggcaaa gccgcgcttg ccgccgcatt
    29881 atcggttgcc gcgcttgccg cagccgccat cctgctcctg tcctgaaacc cgacatcaat
    29941 gccgtctgaa acccttttcg cccttcggac ggcatcccct ctccgcgcaa acggtctgaa
    30001 tgcccgaaac ggcttcaggc cgtttttccg tcccaaaccg ttttatagtg gattaacaaa
    30061 aatcaggaca aggagacgaa gccgcagaca gtacggatag tacagaaccg atttactt
//
LOCUS       DAXHSJ010000004        29112 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000004 DAXHSJ010000000
VERSION     DAXHSJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.004"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(92..880)
                     /locus_tag="ACFM8U_000144"
     CDS             complement(92..880)
                     /locus_tag="ACFM8U_000144"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6333457.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFAGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAESIVPVLQNWARALKTDGLLFFTCFGRDTLAELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYAPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(882..1625)
                     /gene="bioH"
                     /locus_tag="ACFM8U_000145"
     CDS             complement(882..1625)
                     /gene="bioH"
                     /locus_tag="ACFM8U_000145"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC6333458.1"
                     /translation="MPDAAKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTADEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTHDADGIIGRILPDLAR
                     CGTPSALQEALDAAERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRHLKGSRL
                     VVMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            1624..2349
                     /locus_tag="ACFM8U_000146"
     CDS             1624..2349
                     /locus_tag="ACFM8U_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC6333459.1"
                     /translation="MNFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCAADLAAYR
                     TDAANSCPLCFRHIQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIHALKHLADLSM
                     VQPLADLMMQNPPDRLADECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWRIL
                     PRHTVFRHHRPPQSTLKGSERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKQSDANRICCWTLARTPMKK"
     gene            2414..2878
                     /gene="trmL"
                     /locus_tag="ACFM8U_000147"
     CDS             2414..2878
                     /gene="trmL"
                     /locus_tag="ACFM8U_000147"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC6333460.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTAHPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            2889..3011
                     /locus_tag="ACFM8U_000148"
     CDS             2889..3011
                     /locus_tag="ACFM8U_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6333461.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            3008..3727
                     /locus_tag="ACFM8U_000149"
     CDS             3008..3727
                     /locus_tag="ACFM8U_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC6333462.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTTSADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            3781..4107
                     /locus_tag="ACFM8U_000150"
     CDS             3781..4107
                     /locus_tag="ACFM8U_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6333463.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            4189..4773
                     /gene="ruvA"
                     /locus_tag="ACFM8U_000151"
     CDS             4189..4773
                     /gene="ruvA"
                     /locus_tag="ACFM8U_000151"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC6333464.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            4773..4910
                     /locus_tag="ACFM8U_000152"
     CDS             4773..4910
                     /locus_tag="ACFM8U_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333465.1"
                     /translation="MPPEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            4936..6792
                     /locus_tag="ACFM8U_000153"
     CDS             4936..6792
                     /locus_tag="ACFM8U_000153"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6333466.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAY
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VNDGMATLSKPHTILDKPRALPLNVPQGAIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGITQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            6797..7720
                     /gene="rsgA"
                     /locus_tag="ACFM8U_000154"
     CDS             6797..7720
                     /gene="rsgA"
                     /locus_tag="ACFM8U_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC6333467.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETRAASPERLAFLQGITDELPG"
     gene            7747..7836
                     /locus_tag="ACFM8U_000155"
                     /pseudo
     CDS             7747..7836
                     /locus_tag="ACFM8U_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            7945..8208
                     /locus_tag="ACFM8U_000156"
     CDS             7945..8208
                     /locus_tag="ACFM8U_000156"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC6333468.1"
                     /translation="MSKWRGFNRKGNTMKKNAPKSFEEALSRLESLTQAMQGEMPLED
                     ALAAYQEGNELVRYCQTKLAQVEQKLQVLDVDGLKELNLESDE"
     gene            8192..9088
                     /locus_tag="ACFM8U_000157"
     CDS             8192..9088
                     /locus_tag="ACFM8U_000157"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC6333469.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHVQYDEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLDGARAYAYQLTDEAVSLL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(9197..9496)
                     /locus_tag="ACFM8U_000158"
     CDS             complement(9197..9496)
                     /locus_tag="ACFM8U_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC6333470.1"
                     /translation="MTASMYILLLLALIFANAPFLTTRLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(9608..11053)
                     /gene="nuoN"
                     /locus_tag="ACFM8U_000159"
     CDS             complement(9608..11053)
                     /gene="nuoN"
                     /locus_tag="ACFM8U_000159"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC6333471.1"
                     /translation="MNWSDLNLMPAMPEVALLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDDECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(11063..12559)
                     /locus_tag="ACFM8U_000160"
     CDS             complement(11063..12559)
                     /locus_tag="ACFM8U_000160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC6333472.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIYGAIHNPHVAEM
                     KDINCREFAILAILAVVVLGMGLYPQAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(12669..13832)
                     /locus_tag="ACFM8U_000161"
     CDS             complement(12669..13832)
                     /locus_tag="ACFM8U_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC6333473.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIRKVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKVKSWADEIAMKSIPVSEDWL
                     EEYQEAEINYKKSTVSQSLMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKSLIKQ
                     CAAGKIDNDEFYVEFNKLWSWKSENKGEGVGNRFQDQTWKRTGKSSDFRKLCIAIQSV
                     FDAPLAERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEKNK
                     FKSAKGASEGSRYIDLSMKLRALLAMQSEIKNLAELDVLVQAEYRDSTDIDWE"
     gene            complement(14016..16040)
                     /gene="nuoL"
                     /locus_tag="ACFM8U_000162"
     CDS             complement(14016..16040)
                     /gene="nuoL"
                     /locus_tag="ACFM8U_000162"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC6333474.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLFVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPVYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(16089..16430)
                     /locus_tag="ACFM8U_000163"
     CDS             complement(16089..16430)
                     /locus_tag="ACFM8U_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333475.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            complement(16554..17129)
                     /gene="fic"
                     /locus_tag="ACFM8U_000164"
     CDS             complement(16554..17129)
                     /gene="fic"
                     /locus_tag="ACFM8U_000164"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6333476.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            complement(17163..17468)
                     /gene="nuoK"
                     /locus_tag="ACFM8U_000165"
     CDS             complement(17163..17468)
                     /gene="nuoK"
                     /locus_tag="ACFM8U_000165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC6333477.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDGLKG"
     gene            complement(17465..18136)
                     /locus_tag="ACFM8U_000166"
     CDS             complement(17465..18136)
                     /locus_tag="ACFM8U_000166"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC6333478.1"
                     /translation="MTFQLILFYIFAVIILYGALKTVIAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            complement(18172..18879)
                     /locus_tag="ACFM8U_000167"
     CDS             complement(18172..18879)
                     /locus_tag="ACFM8U_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333479.1"
                     /translation="MEGLINALKYLAKHEPIDNFEEIRIRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSIGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            complement(18910..19389)
                     /gene="nuoI"
                     /locus_tag="ACFM8U_000168"
     CDS             complement(18910..19389)
                     /gene="nuoI"
                     /locus_tag="ACFM8U_000168"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC6333480.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(19470..20546)
                     /gene="nuoH"
                     /locus_tag="ACFM8U_000169"
     CDS             complement(19470..20546)
                     /gene="nuoH"
                     /locus_tag="ACFM8U_000169"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC6333481.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGVWMISPLNLWK"
     gene            complement(20549..22810)
                     /gene="nuoG"
                     /locus_tag="ACFM8U_000170"
     CDS             complement(20549..22810)
                     /gene="nuoG"
                     /locus_tag="ACFM8U_000170"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC6333482.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMANRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     TEMANAPKQAVLLLNVEPEIDTVDGSKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRVSSTQKDFQTASSRLVRVGGVGIYHTDAIVRRSVPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGAGVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(23092..23403)
                     /locus_tag="ACFM8U_000171"
     CDS             complement(23092..23403)
                     /locus_tag="ACFM8U_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6333483.1"
                     /translation="MRNHPLTRQPAVYILANGKNGTLYIGVTMNLPERVWQHKNHVNI
                     DGFTARYDVHDLVWYQFFENMPEAVAKEKTMKKWRREWKIKLIEEQNPEWLDLSGVLF
                     V"
     gene            complement(23647..24084)
                     /locus_tag="ACFM8U_000172"
     CDS             complement(23647..24084)
                     /locus_tag="ACFM8U_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333484.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            complement(24084..25385)
                     /gene="nuoF"
                     /locus_tag="ACFM8U_000173"
     CDS             complement(24084..25385)
                     /gene="nuoF"
                     /locus_tag="ACFM8U_000173"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC6333485.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            complement(25775..26248)
                     /gene="nuoE"
                     /locus_tag="ACFM8U_000174"
     CDS             complement(25775..26248)
                     /gene="nuoE"
                     /locus_tag="ACFM8U_000174"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC6333486.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGISPAQAYEVATFYNMYNLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVILVNNHSMCGFMTEEAIEKKLAELE"
     gene            complement(26248..27504)
                     /gene="nuoD"
                     /locus_tag="ACFM8U_000175"
     CDS             complement(26248..27504)
                     /gene="nuoD"
                     /locus_tag="ACFM8U_000175"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC6333487.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVTPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCVDWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(27494..28087)
                     /locus_tag="ACFM8U_000176"
     CDS             complement(27494..28087)
                     /locus_tag="ACFM8U_000176"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC6333488.1"
                     /translation="MASIQNLYETVIGVLGDQASKVISALGEITVECLPEHYVSIMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(28100..28582)
                     /locus_tag="ACFM8U_000177"
     CDS             complement(28100..28582)
                     /locus_tag="ACFM8U_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC6333489.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(28573..28929)
                     /locus_tag="ACFM8U_000178"
     CDS             complement(28573..28929)
                     /locus_tag="ACFM8U_000178"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC6333490.1"
                     /translation="MLSAYFPVFVFILIGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
BASE COUNT         7358 a         8033 c         7115 g         6606 t
ORIGIN      
        1 cactatacat cggtatttcc tttatttctg tcggttttca cagacagatg ccgtctgaaa
       61 cctggaaacg gcttcagacg gcatctgcac atcatctctt cggaaaaaac tgcaccacgt
      121 tctccccttg cggcagcatc agttttttca cggcgtgtcc gtacaccgtt tccagcgtaa
      181 tgcccaaacc gccttcccgt tcaaatattg tcccgacaca cgaacgcgcg gcgttttcat
      241 cgttccacgc catcgcccgc caaacgccca gcgtgtccat atccgcccaa aacgtttccg
      301 cctttttgta atccaacacc agcttcgccg tatcggtaac gggggcgtaa aagccgtttt
      361 cagcaagcat atcgcccaag tcgtgcatat cgggaaacat cacgctgcgg ctttcaatgc
      421 cgttttcttt cagacggcat ttcagttccg ccaaggtatc tcgcccgaag caggtaaaaa
      481 acagcagccc gtccgtcttc agcgcgcgcg cccaattttg cagcacgggg acgatgcttt
      541 ccgccgccaa cagtccgaga ttcgaccaca acatatcggc acacgcttcg ggcagcggcg
      601 cggtcgggga ttggcagtgc tgcaccacgc ccttacccgc aaacctttgc caaaaaccgc
      661 ctttgcgcgc ggctgccgcc gccgccaaaa aatccgcacg ggaatcgtat tcttcaaata
      721 ccgcctgcgg atagcgtttc gccagcaggc tgcggctgat gtccgcatcc gccccggcaa
      781 gcaggatatg tttgggcgcg ttgcggacga gtgtcaggcg ttggtcggca tattcggcga
      841 gatggcggtg aacctgccag catttgtcct gcggattcat gtcaaacccc ttcgacaaag
      901 tcgcggtaca gcgcggcaaa cgcttccgca tggctcaaaa acggcgcgtg tgccgccttt
      961 tccatcacaa ccagcctgct tcctttcaaa tgccgatgca gatactcgcc catgcgcggc
     1021 ggcgtaatcg cgtctttgct accgaacacc agcagtaccg gaacatctat cttgtccaac
     1081 aaatgccgcg catccgccct ttccgccgcg tccaaagctt cctgtaaggc ggagggtgtg
     1141 ccgcagcgcg ccaaatcggg caggattctg cctatgattc cgtccgcatc atgcgtatgc
     1201 aaaagctgta attgtagaaa ctgtttgata tgtttggcat aatccgttcg gaacgcgccg
     1261 accattttgc ccaatgcagg cgcggcaagc ccttcgggat agtcttcgtc agccgtcagt
     1321 cgggcgaaac tcgccgtcag gcagagcgaa cggactttgt cgggatggcg cgccgccaga
     1381 tacagcgcga ccaatccgcc gagcgaccag ccgagaatgt cggcgggcgt atcgatttgt
     1441 gcggcaacag cgtcggcggc tgcctcaata tcgaagggtt gcgcgaaggg cgcgtcgccg
     1501 tgtccgggca aatcgacggc ggacaccggc cacgttgcag gcagacgggg catcaggtcg
     1561 tcgaacacgt ggcggttcgc cccccacccg tgtatcaggt aaactttttt ggcagcatca
     1621 ggcatgaatt ttctctcttg ttggcggcgc atcgcagacg cgcccactat caggcgttgc
     1681 gtattatgcc acggttcgtc cggcgtttca gacggcatct gcgccggctg cgcggcggat
     1741 ttggcggcat accgcaccga cgcggcaaac agctgccccc tgtgtttcag acacatccaa
     1801 ggcggcgcgg tgtgcggcgg ctgtcagaaa aaaccgcccg cattcgaccg gatgtgggct
     1861 tcgctgcatt acgaaccgcc cgtcagcaat atgatacacg cgctgaagca cttggctgat
     1921 ttgagcatgg tgcagccgct ggcagacctg atgatgcaga atccgcccga ccggcttgca
     1981 gacgaatgtt tcgatttcgt cctgcccgta ccgctaagca gggagcggct gctgcaacgc
     2041 gggttcaacc aaagtgaaag catcgtcggg ttgttggcgc aacgctacgg ctggcggata
     2101 ctgccccgac acaccgtatt ccgacaccac cgcccgccgc aaagcacgct caaaggcagc
     2161 gaacggcggc gaaacatcaa aaacgccttt gaaatccgca caccgatacc ggaaaactgt
     2221 aatattttgt taatcgacga tgtctttacc accggcgcga cgctggacga attggcaaag
     2281 acgctgaaac aatcggacgc aaaccgaatc tgctgctgga cgctggcacg cacgccaatg
     2341 aaaaaataac taaatttttt gacaccccat ccgccttgtg gcaaggttac gcgcctataa
     2401 gtgattgata tttatgttta ccatcgtttt ataccagccg gaaatccctc cgaacacggg
     2461 caacatcatc cgcctgtgcg ccaataccgg cgcggatttg caccttgtca aaccgctcgg
     2521 cttcccattg gattccgcca aaatgaaacg cgccgggctc gactaccacg aattcgccag
     2581 cctgacggtg tacgaaaact tcgacgactg cctcaaggca ctcgcaggca ggcgcatttt
     2641 cgccctgacc accaaaggca cggcgcaccc cgatgaaacc gcgtttcaaa aaggcgacgt
     2701 tttactgttc gggccggaaa cgcgcggact gcctgccgac atcctcgaca gcctgcccgc
     2761 cgcgcaaaaa atccgcctgc cgatgcagcc cggcagccgg agtatgaacc tttccaatac
     2821 cgttgccgtg attctctttg aagcgtggcg gcaacacggt tacgcaggcg gcgtttgaac
     2881 gcaagttcat gccgtctgaa aacctatccg gacacattcc gaaccgccgt ccgcactgta
     2941 cggcggttcg gaacgtatcc ggcgggcatg atgcccattc gtcttcaact caagaacgga
     3001 acacgttttg actttaaccc gaaaaaccct tttccttctc accgccgcat tcggcacaca
     3061 ctcccttcag acgacatccg ccgacgcagt ggtcaaggca gaaaaactgc acgcctccgc
     3121 caaccgcagc tacaaagtcg ccggaaaacg ctacacgccg aaaaaccaag tcgccgaatt
     3181 cacgcaaacc ggcaacgcct cgtggtacgg cggtaggttt cacgggcgca aaacttccgg
     3241 cggagaacga tacgatatga acgcctttac cgccgcccac aaaaccctgc ccatccccag
     3301 ctatgtgcgc gtaaccaaca ccaaaaacgg caaaagcgtc atcgtccgcg tcaacgaccg
     3361 cggccccttc cacggcaacc gcatcatcga cgtatccaaa gccgccgcgc aaaaattggg
     3421 ctttgtcagc caagggacgg cacacgtcaa aatcgaacaa atcgtcccgg gccaatccgc
     3481 accggttgcc gaaaacaaag acatctttat cgacttgaaa tctttcggta cggaacacga
     3541 agcacaagcc tatctgaacc aagccgccca aaacttcgcc gtttcgtcat cgggtacgaa
     3601 cctctcggtt gaaaaacgcc gttacgaata cgtcgtcaaa atgggaccgt ttgcctcgca
     3661 ggaacgcgcc gccgaagccg aagctcaggc gcgcggtatg gttcgggcgg tattgactgc
     3721 cggttgatgt cccgccttta cgtctttcaa acaccaccag atacttcgca ggagcaaaac
     3781 atgaacttca aacgccttct cttgaccgcc gccgcaaccg cactgatggg catttccgcc
     3841 cccgcactcg cccaccacga cggacatggc gatgacgacc acggacacgc cgcacaccaa
     3901 cacaacaaac aagacaaaat catcagccgc gcccaagccg aaaaagcagc gttggcgcgt
     3961 gtcggcggca aaatcaccga catcgatctc gaacacgaca acggccgtcc gcactatgat
     4021 gtcgaaatcg tcaaaaacgg acaggaatac aaagtcgttg tcgatgcccg taccggccgc
     4081 gtgatttcct cccgccgcga cgactgaatt tgatacaatc cgtgccgtct gaagcccgaa
     4141 ccggtttcag acggcatttt gcacccgaca cttcaggatt cggcacacat gatcagcaga
     4201 ctgaccggca aactggttga aaaaaaccct ccgcaaatcg tcatcgatgt caacggtgtc
     4261 ggctatgagg ccgacgtatc gatgcagacc ttctacaacc tgccgcccgt gggtgaaagc
     4321 gtacaactgt ttacccagct tatcattcgg gaagacgcac atcttttatt tggttttgcc
     4381 actgcggaag aacgcaagac cttccgccaa ctgattaagg tcggcggcat cggcgcgaaa
     4441 acggctttgg gcattttgtc agcgatgacg gcagacgagc tggcgcgggc ggttgccgaa
     4501 gaagatgtca aacgcctgtc ttccgcgccg ggaatcggca aaaaaaccgc cgaacgtatg
     4561 gtcttggaac tgcgcggcaa gctggtcgcg catacggtaa cggacgggct gtttgccgcc
     4621 gcacccgccg ccgacgaaac ggaagacatc gtcagcacgc tgcttgcgct gggttacagc
     4681 gaacgcgaag caaaagcggc ggtcaaaggc gttccggagg ggacggacgt gggcgaaggc
     4741 gtgcgccttg ctttgaaaaa cctgctgaaa taatgccgcc tgaaggcggc gcggcgtttg
     4801 ccctgacgaa accctgcgtt tccgcctcgc gctgtcttcc cgacggcttt tcccgtaaaa
     4861 tggcgttatt gtccttcctt tcagacggca ttgtgtttga tgccgtctga aactgtttta
     4921 ccgaaatcga aaacaatgtt gaacaaaata ttttcctggt tcgagtcccg aatcgaccct
     4981 tatcccgaag ccgccccgaa aacgccagaa aaaggcttgt ggcggtttgt ctggagcagc
     5041 atggccggcg tgcggaaatg gatagccgcc ctggctgcgc tgaccgccgg catcggcatt
     5101 atggaagccc tggtttttca atttatgggc aaaatcgtgg agtggctcgg caaatacgcg
     5161 cccgccgaac tgtttgccga aaaaggttgg gaactggcgg caatggcggc gatgatggtg
     5221 ttttcggtcg tgtgggcgta tgccgcgtcc aacgtgcgcc tgcaaaccct tcagggcgtg
     5281 ttccctatgc gcctgcgctg gaacttccac cgcctgatgc tgaaccaaag cctcggtttt
     5341 tatcaggacg aatttgccgg acgcgtgtcc gccaaagtca tgcagaccgc gctggcgttg
     5401 cgcgacgcgg tgatgacggt tgccgatatg gtcgtttatg tgtcggtgta tttcattacc
     5461 tccggcgtga ttctcgcctc gctcgactca tggctgctgc tgccctttat cggctggatt
     5521 gtcggtttcg cttcggtgat gcgcctgctg attcccaaat tggggcaaac cgccgcatgg
     5581 caggcggatg cccgctcgct gatgaccggc cgcattaccg atgcctattc caatatcgcc
     5641 accgtcaaac tcttctccca cggcgcgcgt gaagccgcct atgccaagca gtcgatggaa
     5701 gaatttatgg ttacggtgcg cgcccaaatg cggctggcga cgctgctgca ttcgtgcagc
     5761 ttcatcgtca acacctccct gaccctctcc accgccgcac tgggcatctg gctttggcac
     5821 aacggacagg tcggcgtggg cgcggtcgcc accgccaccg cgatggcgtt gcgcgtcaac
     5881 gggctgtcgc aatacattat gtgggaatcc gcgcggctgt ttgaaaacat cggcaccgtc
     5941 aacgacggta tggcaaccct gtccaaaccg cacaccatcc tcgacaagcc ccgggcactg
     6001 ccgctgaacg tgccgcaagg cgcaatcaaa tttgaacaca tcgatttctg ctacgaagcc
     6061 ggcaaaccgc tgctcaacgg cttcaacctc accatccgcc cgggcgaaaa agtcggcttg
     6121 atcggacgca gcggcgcggg caaatccacc atcgtcaacc tgcttttgcg cttctacgaa
     6181 ccgcaaagcg gcacggtttc gatcgacggg caggacataa gcggcatcac ccaagaatct
     6241 ttacgcgccc aaatcggttt ggtcacgcaa gatacctcgc tgctgcaccg ttccgtgcgc
     6301 gacaacatta tttacggccg ccccgacgcg accgatgccg aaatggtttc cgccgccgaa
     6361 cgcgccgaag ccgccggctt catccccgaa ctttccgatg ccaaagggcg gcgcggctat
     6421 gacgcgcacg tcggcgaacg cggcgtgaaa ctctccggcg ggcaacgcca gcgcatcgcc
     6481 attgcccgcg tgatgctcaa agacgcgccc atcctgctgc tcgacgaagc caccagcgcg
     6541 ctcgattccg aagtcgaagc cgccatccag gaaagcctcg acaaaatgat ggacggcaaa
     6601 accgtcatcg ccatcgccca ccgcctctcc accatcgccg caatggacag gctcgtcgtc
     6661 ctcgacaaag gccgcatcat cgaagaaggc acacacgccg aactcctcga aaaacgcggg
     6721 ctttacgcca aactctgggc gcaccagagc ggcggcttcc tcaacgaaca cgtcgagtgg
     6781 cagcacgact gaaccgatgc cgtccgaaca cccgttttca gacggcattt ccacacccaa
     6841 ccccaaagaa accatgaacg acaccgccca aattaccgcc agctacggcc gccgctacat
     6901 tgtccgcacg cccgacggca caacctacga agccagcacc cgcaaaaaac gcgtcgattt
     6961 cgcctgcggt gaccgcgtcc gcatcagccc cgtcaacgcc gaacaagttg tgattgaaga
     7021 ttttttaccg cgccaaagcc tgctctaccg ccaagacgcg tggaaaacca aactcattgc
     7081 cgccaacgtt acccaactcc tcatcgtaac cgccgccgtc ccgagtccga gcgtgcggct
     7141 gctacagcgc gccctgcttg ccgccgaagc cgccggcatc cgcgccgtca tcgtcctgaa
     7201 caaagccgac ctgcccgaaa ccgccctttg gcgcgaaaaa ctcaaattct acgaaacgct
     7261 gggttatccc gtcatcgaaa cccgcgcact ggaaaacgcc ggcagcctgc gccccgccct
     7321 gcaagggcac agcaacatcc tgctcgggca gagcggtatg ggcaaatcca ccctgaccaa
     7381 cgcccttttg ggcagccaaa ccgcccgcac cggcgacatt tccgccgcac tcgactcggg
     7441 aaaacacacc accacccacg cccggcttta tgatttgaac ggcgaaaccc aactcatcga
     7501 ctccccgggt ttgcaagaat ttggtttaca ccacctccaa gccgccgatt tgccgcgcta
     7561 tttccccgat ttccgccacc ttgtcgggca atgccgcttc cacaactgca cccaccgcgc
     7621 cgaacccggc tgcgccttca aagccgccgc cgaaaccagg gcggcaagcc ccgaacgcct
     7681 cgcctttttg cagggcatca ccgacgaact gcccgggtaa cgccttgccg tttgcaggac
     7741 aaaacaatgc cgtctgaaac cggattcggg tttcagacgg catccgtttt tcaaaaatgc
     7801 tacaatccgc tttttaccgg aacatccgaa actatgttcc aacacgcagg acgacactta
     7861 aagcaccgcc ctatgtgtcg tcctgatttg gaagggctta cacccctccc aaataaagtc
     7921 tgattctacc gccccgaagg acagatgtcc aagtggcggg gtttcaaccg aaaaggaaac
     7981 acgatgaaga aaaacgcccc gaaatcgttt gaagaagcct tgtcgcgcct tgaatcgctg
     8041 acacaagcta tgcagggcga aatgcccttg gaagacgcgc ttgccgccta tcaggaaggc
     8101 aacgagctgg tcagatactg ccaaaccaag ctggcgcaag tcgaacagaa attacaggtt
     8161 ttggatgtgg acgggctgaa ggagttgaac cttgaatccg acgaatgatt tgaaagcgtg
     8221 gcaacagagg gcgcaggcac agacagagct gctgctggaa cgctttttgc cgtctgaaaa
     8281 cgaaatcccg cacacgctgc acgaagcgat gcgctatgcg gctttggacg gcggcaagcg
     8341 tctgcgtccg atgctggtgc tggcggcttc ggaattgggc ggagccgtgg cggatgcggt
     8401 cggacaggca atggcggcaa tcgaaatgat ccacgtctat tctttggttc acgacgatat
     8461 gccggcgatg gacaacgaca gcctgcggcg cggcaaaccg acttgccatg tgcaatacga
     8521 cgaagccacc gcgctactgg tcggcgacgc tttgcaaacc caagccttcg acgtgttaag
     8581 ccgtccgaca gaactgcccg cagcacgcca gttggcaatg ttgtcggtgt tggcgaaagc
     8641 gggcggcagc gcgggcatgg cgggcggtca ggcaatcgat ttggcgaatg tcggcaaaca
     8701 aatggttcaa gccgatttgg aacagatgca cagcctgaaa acgggtgcgc taatccgtgc
     8761 ggcggtttta ttgggggcga tgtcctgccc gaatctgtct gaagacagtc tggcggtatt
     8821 ggacgattat gcggcaaaac tggggctggc gttccaagtc attgacgatg tgttggattg
     8881 tgaagcggac acggcgactt tgggcaaaac ggcgggcaaa gacgcagaca acgacaagcc
     8941 gacttatgta aaactgatgg gcttggacgg tgcgcgggca tacgcgtacc aactgaccga
     9001 cgaagcagtc tcgctgctcg aacccttcgg cgacaaagcc ctgcgcctgc ggcagttggc
     9061 agaatttgca gtcgcccgca aatattaaaa ccggcgtata tcgccggctg aaacgtgccg
     9121 gcacagcgat gccggcctgc acttgaatga aacaaatgcc gtctgaaaag gtttcagacg
     9181 gcatttttat gcttgtctat tccctgttgc gcgtgtgcca aaaataccgc cacacgaaac
     9241 agggaaacgc aaaaatcagg tacaggcaga cgacggtggc gtaaaactcc caaccctgat
     9301 cgtgtaccgc tcccgcacgg gattcgagga tgtaggcaag aacggcggtc agcgcgaaac
     9361 ctgccgccag ctcgatcaag tggtgcccga aatgtttgcg cttgagcggt acgatgccga
     9421 acagcctggt cgtgaggaag ggggcgttgg caaaaatcaa ggcaagcagc aaaaggatgt
     9481 acatggatgc ggtcatggcg ggctttctgt ttgggcattg tcggcggcga tgccgtctga
     9541 actgcgttgc ggaacggcat atcttaacaa aaacggcagc ctctgacacg gctgccgttg
     9601 cggcggctta cagcgtgttt tccaacgcct tggcgcacca gtcgataacg gtttgcggca
     9661 taatgcccca cagcaccaac agcagcgcgt tgacgctcaa aaggaatttt gcggcattgt
     9721 tgccgccgac ggtgggacgg gcgcggccgg attcgtcaaa atacatgact ttgaccacgc
     9781 gcaggtagta gaacgcgcca atcagcgaca tcacgacggc aaatacggac aaccaaacat
     9841 agccttgttt taagagtgcc ataatcacgc cgaatttggc gtaaaagccc atcagcggcg
     9901 gaatgcccgc catagagaac ataaccagca gcatcaaaaa ggcaagccat acgcggtgtt
     9961 ggttcaaccc tgccaaatcg ctgatgtttt cgcactcgtc gtccccgtcc gacaacacca
    10021 tcaacacgcc gaaccctgcc gccgccatca gcgcgtaggt aatggcgtaa tagaggcccg
    10081 ccgcaaagcc gaccgcgccc gccataaacg ccaacaggat gaaacccata tgcgatacgg
    10141 tggaataggc gagcatacgt ttgatattgg tctgcatgat ggcggcaagg ttgccgacca
    10201 gcagcgaggc ggcggcaagc agggcaaaca tcagagacca gtcatgatgc acggttccca
    10261 gcccggtaac gaggatgcgg aaagcgaaaa cgacggcggc gattttcggg gcagtgccga
    10321 ccaaggcggt aacagaagtg ggcgcgccgt gatacacgtc gggcacccac atatggaacg
    10381 gcaccgcgcc gagtttgaac gcaacggcga cgacaataaa caccaaaccc agtttcaaca
    10441 gccattcatt ggcttcttca ttgaaggaag aggcgagcac gccggcaaat tccagcgaac
    10501 cggttgcgcc gtaaaccata gaaataccgt agagcagcag gccggatgcc agcgcgccca
    10561 aaacaaaata tttcaaggcg gcttcggcgg caaagccgga atcgcggcgc agggcaatca
    10621 gggcgtaaag ggcaagcgac aagagttcca aaccgatata ggcagttaaa aaatgccccg
    10681 cgctcaccat cacgctcatg cccaagaggg caaacagcga cagggtgtaa aactcgcctt
    10741 tgaaaatacc gcgcacttgg ttgtagggct tggcataaac aaacagggca aacgtcaagg
    10801 catataaaac catttttgcc aaacgcgaca taccgtctgc aatatacatg ccgttgaacg
    10861 aagacgtgct gccctgttcc caaacggcaa gctgcaccac agccgtaacc gccaccgttg
    10921 ccaacgcgcc gtaatgcgtc caacggcgtt tgtcatcact gacccacaag tctgccagca
    10981 acaataacac cagcagcgac agcagcgcga cttcgggcat ggcgggcatt aaattcaaat
    11041 cagaccagtt catttacaca cctcaaatct tgctttgtgc cacatgggca atcaaatcgt
    11101 ttgccgcctg atgcaccact tcgataaacg cttgcggata cagacccata cccaatacca
    11161 caaccgccaa aatcgccaga atcgcaaatt cgcggcagtt gatgtctttc atttcggcaa
    11221 cgtgcggatt gtggatcgcg ccgtaaatca cgcgcttgta catccacagg gtataagacg
    11281 caccgtaaat caaggtcata gccgccagcg cgccgaccca gaaattcact ttgaccgcac
    11341 ccataatcac cataaactcg cccacaaagc cggaagtcgc aggcagaccg gcgttcgcca
    11401 taccgaacaa catcataaac gccgcaaact taggcatcac attgaccacg ccgccataat
    11461 cggcaatatt gcgggtatgc aggcggtcgt acatcacacc gatgcacata aacatcgccg
    11521 ccgacacgaa accgtgggaa atcatctgaa tgaccgcgcc tttcaatgcc cagtcgtcca
    11581 actgcccgtc aacaaacaaa aacatcccaa gcgttacaaa acccatatgg ctgatggacg
    11641 aatacgccac cagttttttc atatcggttt gcaccaaagc caccataccg atataaatca
    11701 cggcaatcag actcaatacg atgatcacgg gggcaaaata gcgtgccgca tccggcataa
    11761 tcggcaggat aaagcgcaag aaaccatacg cacccagttt cagcgtaatc gccgccaaca
    11821 ccatcgaacc gccggtcggc gcttcaacgt gggcatccgg cagccaagtg tgtacgggga
    11881 acatcggcac ttttacggca aacgacagga agaacgccgc aaacaaaagc tgctgtacgc
    11941 ccaacggaat ctgtttaatg ttttggaaat cgacaataga gaagctgcct gtttgatagt
    12001 acaggtaaac catagcaacc agcatcagga gcgaacccat cagcgtatag aggaacagct
    12061 tgaccgacgc atagacgcgg cgcgggccgc cccatacacc gataatcagg tacagcggaa
    12121 tcagcatacc ctcgaagaac acataaaaca gaatcgcatc ctgcgcggca aacgcaccgt
    12181 taatcaaacc cgacatcatc aggaatgccg ccatatactg cgccgggcgt ttctgaataa
    12241 cttcccaacc cgccagcacc accaacagcg taataaacgc attcaagatg ataaagagca
    12301 ctgaaatacc gtccacgccc aatgcatagt tgattttcag cagcggaatc cactcgtgga
    12361 actcggtaaa ttgatagccg ccgctcaaac ggtcgaaacc ggtaaacagg ggcagcgtta
    12421 ccaagaaacc ggcaagcgca cccataaagg caagcacacg cgccagcggc gcacggctgt
    12481 ccttaccggt cgacaaaacc agcacacctg cggcgatggg tatccatatt gccaagctga
    12541 gtaggtagtt ggaaaacata gtggttaacc tgtggttaaa ataaaaatat cgtagtgcgg
    12601 gttagtttgt cagacgacgt ttcaggaggt cgtctgaaaa agggatgaat actctctccc
    12661 ctctttactc actcccaatc aatatctgta ctatcgcgat attctgcctg cactaaaaca
    12721 tctaattcag ccaaattttt tatttctgac tgcattgcta gtaaagctct taacttcata
    12781 cttaaatcaa tatatctaga gccttcactt gctccttttg cagacttaaa tttatttttt
    12841 tctaaaaatt ttttaatagg agcgtttaac actggatatc tatctggata ttcttgacaa
    12901 agcatttcag aaaaaactga accacgagtg gaaactccac attcttttag ccaatcaatt
    12961 tgcttggcaa caacattatc tctctcagca agtggtgcat caaaaactga ttggatagca
    13021 atgcataatt ttctaaaatc tgaagacttt ccggtccttt tccacgtttg atcttgaaat
    13081 cgattaccta ccccttctcc tttattttcg cttttccaag accataattt attaaattcc
    13141 acataaaact catcattgtc tatctttcct gcagcacatt gcttaataag actttttaat
    13201 tgattacaca cagtttgatg gtttctcatc tgttttctcc gtgctgcaat cagttcttga
    13261 ttataatttg gcatttccat aaataactta tccatcaatg attggctaac agttgatttc
    13321 ttataattaa tttcagcttc ctgatactcc tcaagccaat cttccgatac tgggatagat
    13381 ttcatcgcaa tttcatcagc ccaagatttg acttttataa aatcctgctc ggaaatttta
    13441 gtcaaaatat ttgcttcata attgctatta aacgcagcat gagtcaaatt tgaagaaccg
    13501 attaaagcat aatattcatt cttatcattc ttccaaaata cagctttagg atgaaaaccc
    13561 tgaatgcctt gagctaccat cagtgtgaat ttcaaatggg acggaagcca tttcaacact
    13621 ttccgaatag cctctttttt agacaatccg aaatcttgtc caaaaataaa tttaaactct
    13681 tgcaatttgc tattgagctt attttttaca tcccaatgag tgagatacgc agagaaaata
    13741 tacagctcta ctgcctctga aacagctttc tcatatattg aaatcagttc cttctcattt
    13801 ggtgtatctg aatgtaaaaa taactgtgtc atcccttttg actcctctat atttgttgag
    13861 attgcctaaa aaatttagga aacagataat ttgcatgttg gctatttcac gcctgttggg
    13921 taccattttg ttccaccctt tgacttaatg cccacttctc aaacttttca gacgacctct
    13981 acaaaactat gaacaggtcg tctgaaactc cgcctttatc tgaacaatcc ccagaaggtc
    14041 atgccgagca gtaccaatac gccgaatacc atagcggcag cgtaagtgta gataaagccg
    14101 gtttgggctt tacgcacttg cgcggcaatc gcgccgacca gtttggcaga gccgttgacg
    14161 atgccgttat cgataatggc ggtatcgccg actttccaga agaaggtacc cagtgcgcgc
    14221 gtgcctttag cgaagacgtt gaaatacagg gcgtcgaggt agtatttgtt ttcaaacaaa
    14281 acgtaaaccg gacggaacgt ctgtgcaatt ttcgctggca ggtgcggcag tttgacgtac
    14341 aaaagccatg cgctcaacac gcctgcgata gcaaggtaga gtacgggcga atgcaggctg
    14401 tgcgacacca ttgccaatgc gccgtggaac tcttccttca tgatgtgcat ggtcggatgc
    14461 gcgtcggcgt tgacgaaaat cacatctttg aagaaatcgc cgtaaagcat aggctcgatg
    14521 gcaacgtagc cgatgatgac ggacggaatg gcaagcaaaa tcaaaggcag ggtaacgaac
    14581 agcgggcttt cgtgcggatt gtcgtttttg cccaagccgt gatgttcttc gccatgtccg
    14641 tctgaatggt gttcgggcag gctgcgccat ttctcttcgc cgtggaacac cataaagtat
    14701 tggcggaacg cgtaaaacgc ggtaacgaac acgctggcaa ggacggcaaa ataggcaaag
    14761 ccgctgccgg gaagggtgct gtatttcgcc gcttcgataa tcgaatcttt ggagtaaaag
    14821 ccggagaaga acggcgtacc aatcagcgac aagttaccga tcaacatggt caaccaagta
    14881 atcggcatgt attttttcag gttgcccatg tggcgcatgt cttggtcgtg atgcataccg
    14941 ataatcgcgc tacctgcggc caagaacaac aaggctttaa agaaagcgtg ggtcatcaca
    15001 tggaacatcg ccacggaata ggcagacgcg cccagagcca cggtcatgta gcccagttgc
    15061 gacagggtag aatacgcaac cacacgtttg atgtcgtttt gaatcacgcc caaaaaaccc
    15121 ataaacaggg cagtaatcgc accgattacc atgatgacag acagcgcagt gctgctcatc
    15181 tcataaatcg gcgacatgcg cgacaccatg aacagacccg ccgtaaccat cgtcgcggcg
    15241 tgaatcaatg cggaaatcgg ggtcgggcct tccatcgaat cgggcagcca gacatgcagc
    15301 gggaattgag ccgatttacc cattgcgccc acaaacaaga gcaaacaagt tacggtaatc
    15361 aaagaccatt ccacaccggg gaaaagctgg atggtggcat tttggacgtt gggcagatag
    15421 gcgaatacgt cctgatagcg caggctgccg ccgaaatagg caagcaccaa gccgataccg
    15481 agcaaaaagc cgaagtcgcc gacacggttg atcaaaaagg ctttcaggtt ggcaaatgtt
    15541 gcgcttggac gtttgaaata gaaaccgatc aagagatacg acaccaaacc cacagcttcc
    15601 cagccgaaga agagctggat gaagttgttg ctcataatga gcatcaacat gctgaaagta
    15661 aacaaagaaa tgtagctgaa gaagcgttgg taaccgactt tttcatcgtg catatagccg
    15721 atggtataga tgtgtaccat caacgatacg ccggtgacca cgaccatcat catcgccgtc
    15781 atcgtatcga ccaagaagcc gacggagaaa tccaaaccgc ccatcgtcag ccaggtatag
    15841 acgttttcgt caaacttggc gcggctgccg tcgataaagc cccacagcac ataagccgac
    15901 aacacggcag acaccgccac accgagtatc gtaaccgtat gcgcgccggc acgtccgatt
    15961 ttgttgccga acaaacccgc gatcagtgag cctgccaaag gcacaagggc aatgatgaga
    16021 tataaagtca tgtcgttcat ttgattgaat ccgattgatt taaaaatcta tgtttgtttc
    16081 gtacaaaatt acttcggaaa aacaaatcca acacgctcca atcgtttgcg tgccacagct
    16141 aattgctctt cagtaaataa atcacaccac ggcttttgta acaccagata ttccatactg
    16201 tatttcaagg cgtggactcc gccaccactc aaaatcagct ctgtaaaacc ggtctgagtc
    16261 ttcttttccc ccgtactcaa taatttatcc gccgcctctt taccaccaaa ttcatttaca
    16321 atttgtaaaa atcgtgtcgc cttgtaaggt tgcggcaaat tcaaagcctc ctgataaata
    16381 tttaacatgg ctttatgaaa ttcttgttct aactgatttt tatccatcat tcttcttcca
    16441 atatttcaga ccggattatt cttacccaga atttcttttc tcatccgctc ccgtctgatc
    16501 acctaccaaa tcaggtcgtc tgaaacaatc tgaaatcgct tttcagacga ccttcagcct
    16561 ttttcatacc cttcgtaata atatgactgc tcgatacctt taaagatgat ttcacggttg
    16621 tccacatcgt cggtcaggtt gtcctttaac aaaaagcgca gttctaaatc gttgacgggg
    16681 ctgcgttcca tcgcctgcaa atacagggtt ttgcttacat tttgccagtt cacgactttt
    16741 ttcaggtttt tcttcagcac caaatccagc cagatgcggg tacttctgcc attaccctcc
    16801 aaaaacggat gggcaatgtt catttcaaca tatttggcga tgatttcttc aaaagtccgc
    16861 tcgggcatct gctcgatttt aaccaaagcc tcttttaaat acatggcgtt ggcaaaacga
    16921 aaaccgcctt tggaaatgtt gtcttccctg atttgacccg caaaatcata taagccgccg
    16981 aacaggtaac ggtgaatctg ttgcaggccc gcggtggtac cgacttcgat acggtcgatg
    17041 tcgccgcttt caaacaggcg gcgggcatta tgcaggcttt gttcgtctat ggatttcatc
    17101 gtttttccta tcgggttttc agacggcatc ggtgtcttgt caggtctcga cccaacctac
    17161 ctttaccctt tcaacccatc caaatcggca acattaatcg tttgtcggtt gcggtacacc
    17221 aataccataa tcgccaaacc gatggcggat tcggcagcgg caacggtcaa tacgaagaat
    17281 acgaaaattt gtccggcagt atcgcccaaa tgttgcgaga aggcgataaa gttgaagttc
    17341 accgccaaga gcatcagctc aatggacatc aacaatacca gcacgttttt gcggttcata
    17401 aagataccca ttgcgctgat accgaacagg agcgcaccca ataccagata atgcgtcaag
    17461 gtaatcatgc tttgccctcc ccttccggct tgaggccgtc tgaaacttcg ctttcttcgg
    17521 cagattcgac ttgcggtttg accgcttcca ttttcaccag acgcatacgg ccttgatcgg
    17581 cgcgtacttt aacttggtct gcagggtcca tacgtttcgg attaaccgtt ttacggtgaa
    17641 ccagcgcaat cgccgccacc atacccaaca gcagcaatac cgcagccaat tcaaacggca
    17701 acagatagtc ggtataaata cggctgccca aatcgcggat attgttgtaa tcggccggaa
    17761 tgtctttcat caggccaaat gcggcaaggt cggttttcgg gttgaccaaa atcagaatca
    17821 gggcaaccgc caacaacgtg ccgaccacac cggcaacagg cgcatgccgc cagaaaccgg
    17881 cacgcatttc ttcaatgtcg atgttcaaca tcatcacgac gaacaggaac aacaccatca
    17941 cggcgccgac gtaaaccacc accagcgtaa cgcccaaaaa ctcagcctgc atcagcatcc
    18001 aaagcatcgc gctcacgcag aaggtcagca ccagatgcaa agcggcgtga acggggtttt
    18061 tagcgatgac ggttttgagc gcgccataga gaattatcac tgcaaaaata taaaataaaa
    18121 tcagttggaa agtcatagtc tatgctttct ttattaatca aaaatatggt ttcaagcagt
    18181 ctgaaaatcc tggttacgca aacaatcaaa cttaatcagt tgatccatat ccgcacaaat
    18241 ccacgttgtc gcaattttga ataacaacat ttctatgtga tacggttttg cttgtttacc
    18301 ataaacttgt ttcgccaaat cttgtaacaa tccacaatat tgatgaaacg aaacgtctgg
    18361 tttccgataa tttggtttga tatcagaaaa atgaaacaat acgttcatat ttcggatttc
    18421 tagttcccta tttctacctt gtggctgaaa aaacaaatct gcatttgtaa atttaaacaa
    18481 caaccaattc aacgaaaaaa tagcgcgtga atcataaatg gcgaatcgct cacaatctat
    18541 aaaagaagcc aatttagata atgatgaaat tttcaacaaa ccaatactca attttcccga
    18601 attaagttcc gataaaaatt gacgaataag ctggctattt tcagcagatt gtttaaaact
    18661 tttaatccca ccccattcac ggataatcca ataatgcgtc ttcaagctat catcattttg
    18721 taattttggg gataaatttt cttttaaaaa aatattttgt tcaaaattac ttaatccact
    18781 tggcaactca atcggactat ttctaattct aatttcttca aaattatcta ttggctcatg
    18841 tttggctaaa tatttcaatg cattaatcag tccttccata tttaaagcct tccaagaagc
    18901 ccaaaagcat taacgatacg gcgcgtcagc ggctttacgt ttggcgattt cggcttcgta
    18961 tttgtcgccg attgccaaga gaatcggctt ggtcatgtgc aagtcgcctt ttttctcgcc
    19021 gtggtattca aaaatatggg tttctacaat cgcatcggtc gggcaggcct cttcgcagaa
    19081 accgcagaag atgcacttgg tcaggtcgat gtcgtaacgc ttggtgcggc gggtgccgtc
    19141 ttcgcgttct tccgattcga tgttaatcgc cattgccgga cacaccgcct cgcacaattt
    19201 acacgcgatg cagcgttcct cgccgttcgg ataccgccgc tgcgcgtgca gaccgcggaa
    19261 acgcacggat tgcggcgttt tctcttcggg aaaataaatt gtgtctttgc gggcgaaaaa
    19321 gtttttgagc gttacgccca tgccttttac caactcgccg agcagaaagg tttttactaa
    19381 gttagccata ttatgttccc tcaaaacagg gatttcgtta ggtattcaaa atcgctttgt
    19441 tcagacggcc tcaagatgcc gtctgaaact tatttccaca aattcagcgg tgaaatcatc
    19501 cacacgccca aaatcacgat gtaggcgaag ccgatcggaa tcagcacttt ccagcccaaa
    19561 cgcatgattt ggtcgtaacg gtagcgtggg aaagttgcac ggatccacag ataccagtac
    19621 agcaccgccg ccattttcac gaacatccag aatgcggaag gcgtaccgat aaagccccag
    19681 ctttgcggga acggagacag ccagccgccg agaaacatca gcgatgtcag cgcggcaatc
    19741 agaatcatga aaatgtattc ggcaaggaag aacagcgcga atgcgaagcc ggaatattca
    19801 acgtggtgtc cggcaacgat ttcggactcg ccctctgcca cgtcaaacgg tgcgcggttg
    19861 gtttcggcaa cggcggaaat cagatagacg atgaagattg ggaacagcgg cagccagttc
    19921 cacgagaata cggaaccgcc cgcgatacct tttgcctgcg cggcaacgat gtcggagaag
    19981 ttcatgctgc ccgacaccat cacgacacac accagcgcgg cactcatggc aatctcgtag
    20041 gaaatgcttt gtgcggaagc acgcattgca cccaagaacg aatatttgga gttggaagcc
    20101 cagcccgcga tgatcacgcc gtaaaccgac agcgaggtaa tcatcagaat gtacaaaaga
    20161 ccgatattga tgttggtcag cacccattct tcgttgaacg gaatcactgc ccatgccgcg
    20221 aaagacggag caagcgacat aatcgggccg atatagaaca gggctttgtt tgacagcttc
    20281 ggacgggtta cctctttaaa caagagtttg aacacgtcgg caaacggctg aatcagaccc
    20341 catgggccgg ttacgttcgg accgacgcga agctgcataa agccgatgac tttacgttca
    20401 aaataagtca ggtaggcgac ggtcagaata agcggaatca gaataatcac gattttgacg
    20461 atgacggata ccaccaagcc tacggtgatg cccaaatcgc ccagaccgag cgttgcggca
    20521 aagaggtttt ggaaccattc ctgcataatc aagctcccgc cagttcaata gtgtccatca
    20581 acgcacccag cgcggcattt tcggtatgca gcggcagatg caccacgttt tcaggcaaac
    20641 cggcatcggc tttgactgca accgatacgc ctgcgccgtt ttgtttggcg acggcggttt
    20701 gtccgtcttg caggcccaag cgtgccaatg tgttcggatt tacacgcgcg gcaggcacgg
    20761 cggcatggct ggtttcttgc aacggtacgg aacggcgcac gatggcatcg gtgtgataga
    20821 tgcccactcc gcccacgcgc acgaggcggc ttgatgccgt ctgaaaatct ttttgagtcg
    20881 aagacacgcg gttgtccagt ttggacggca ggctttccgc gtccagcgcg tctttcagaa
    20941 tcgcagcggt atcgtgatat tcaaaacctt tcaggtcaaa caggttgccc aatacgcgca
    21001 acactttcca cagcggacgc gagtcgccga agccttgtac cacgccgtgg aaggattgca
    21061 gacgaccttc catattgatg aagctgcccg aggtttcggt aaacggcgca atcggcaaca
    21121 acacgtcgca cacatccagc agcgtttcgc tgacaaacgg tgtaaacgcc atcacgcttt
    21181 tcgcctgttt caacgcggcc acggcttttg aaccgtcaac cgtatcgatt tccggctcga
    21241 cgttgagcag caagactgcc tgtttcggcg cgtttgccat ttcggtaacg ctctcacctg
    21301 agttcacgcc caatacgtcc gcaccgacgc tgttggcggc ttgcggcaaa atacccagta
    21361 ccgcgccggt tgcatccgcc agctcttgcg cggcggcata aatggcggcg taatcaggat
    21421 ggttttgcac ttccgcgccc aaaatcaccg ctgctttttc agcgtttttc aggctggcgg
    21481 taacggcgtg ttccgcattg gcagacaggt ttttcagacg gcctgcccac tcgtcgggat
    21541 gtgcggcttc ttgagagaga agcggcatca acaactcttc tttactgccg gccaatacac
    21601 tcaacgccat acggtctttg gcggcgcggc gcaggcgggc ggtcaggagc ggctgttctt
    21661 tgcgcaagtt cgcgccgact accaatacgg catcgttgtc agccaaagat tcaatgcttt
    21721 gtcccaacca ttgcgcacct ttaatgccgt ctgaaagacg tttgtcttgt tggcgcagac
    21781 gggtggcaaa gtttttaaca cccaaaccgt cggcgagttt tttggccaga tacagctctt
    21841 caaccgtatt catcgggttc gcccaaatgc cgacttggtt ttggttgccg tctttggcga
    21901 tacattcaat cgcgctgcgg acatattcca acgcggtttt ccaatccacg tccatccact
    21961 cgccaccctg tttgattttc gggtttttca gacggctttc gtgatacagg ccttcatagg
    22021 cgaaacggtc gcggtcggac aaccagcatt cgttgatggc ttcgttttcc aacggcaaca
    22081 ctcggcggac ggtatggtct ttggtttgca caatcaaatt gctgcccaaa gcgtcatggg
    22141 cggaaacgga tttgcggcgg ttcaattccc aagtacgcgc gttgaaacgg aatggtttgc
    22201 tggttaatgc gccgacagga cacaaatcga tgacgttgcc cgacaattcg gtttccaccg
    22261 ctttgccgat aaaaggcatg atttcggaat gttcgccgcg attcgccatc gcgatttcct
    22321 gcaaaccggc gatttcttcg gtgaatcgca cgcaacgggt acagtggata cagcggctca
    22381 tctcctcggc ggaaaccaaa ggacccatgt ccttgccgac gacggaacgt ttttcttcgg
    22441 tatagcggct ggtggttttg ccgtagccca ccgccaaatc ctgcaactgg cattcgccgc
    22501 cttggtcgca ggtcggacaa tcaagcggat ggttgatgag caggaactcc atcacgcctt
    22561 cctgcgcctc ccgcgccttc gccgaatgcg tgcgcacaat catgccgtcc gttaccggcg
    22621 tagcgcaggc gggcagcggt ttgggggctt tttccacgtc caccagacac atacggcagt
    22681 tggcggcgat ggaaagtttt ttgtggtaac agaaatgcgg aatataagta ccgagcttgt
    22741 gcgcggcttc aatgaccgtc gcgccctgct ccacagatac ctgtttaccg tcgatttcga
    22801 tttgtaacat ggttcgttcc tagttacggg tatttgataa ataatctttt atggaggtcg
    22861 tctgaagaat gggtttcaga cggccttttg agtttcattg acaatcaata ctgtttaaaa
    22921 cgtttactgc caccttgccg tcattcccgc gtaggcggga atccattctt gggttttcgg
    22981 taactgtttt caaacattgg tttcttaact tttgcggtga ttcccgccgt agcctgtctg
    23041 cgcgtaggtg gggctggaat aacgacagtt tttaaagttc cagaaataaa actaaacaaa
    23101 caacacgccc gacaagtcca accattcagg attttgttct tcaatcagtt taatcttcca
    23161 ttcacgtcgc cattttttca tcgttttttc tttggcaact gcttcaggca tattctcaaa
    23221 aaactgatac caaactaaat catgcacatc atatcgagca gtaaagccat caatattgac
    23281 atggtttttg tgctgccaaa ccctttccgg caaattcatg gtaacaccga tatagagtgt
    23341 tccattttta ccgtttgcca aaatatatac ggcaggttgt ctagtaagcg gatggtttcg
    23401 tattcatttt tcctgcaata taaacaacaa cttttttaat atcatttaca accgttttca
    23461 tctgaaaaca tctgcctact cccgccgtca ttcccgcgta ggcgggaatc cattcttggg
    23521 ttttcggcaa ctgcttttca aatatcggtt tctttagatt ccactatgga ttcccgccta
    23581 cgcgggaatg acggcaaagt taaattttta gcattttgct tttaaccaat ataaaaccaa
    23641 ctaaaactat aactcattta ttatattttt aaatgactta aataactctt taacagcacg
    23701 ctcattgtct tcatctgtag gctcaaactt ttcacaccaa ttaattatat ttacatcgcc
    23761 atgcaataac tctgcataac atcctactgc atcgcaacta ccaccccgtg tccttgacgt
    23821 gccaaaaata aaatcacaat tatgcgaata aagtttgtat aaatttctcc taacctcgta
    23881 ttcattatca ccagatgaat aaaaaccaat cctctgacct tcatgatcaa atactgcaac
    23941 aaaatctaag ccgtttccat gtctttcaaa aaagactaga aatttagaaa atttccgaca
    24001 aatctgatta aaaagcgtat tgagtgtcgt actcttacct ttgttggctg caccgtatag
    24061 aataaaaatt ttagcttcca ccattaccac cctccccact tatgctcttt catcggtccg
    24121 ccgtgttcga tgtaatgcac aaactcatca cggaaatgct tggtaaagct gcggacgggg
    24181 aaaacggcgg catcggcaag ggcgcagatg gtgcggcctg ccatttgatt gccgacggaa
    24241 tccagcaaat ccaaatcttc cattttgcct ttgccttcta cgatgcggtg gacgatgcgg
    24301 tagagccagc ccgtaccttc tcgacaaggc gtacattgac cgcaagactc gtcatagtag
    24361 aagtagctca gacgctcaag ggctttcacc atgcacacgt cttcgtccat gacgataatc
    24421 gcgccggaac ccaacatcga gcctgcttta gaaatcgagt catagtccat attggtctgc
    24481 atcatgatat cggcaggcaa tacaggcgcg gacgaaccgc cgggaataac ggccttgagt
    24541 tttttgccgc cgcgcatacc gcctgccatt ttcaagactt cggcaaacgg tgtgcccaat
    24601 ggcacttcat agttgcccgg acgctcgaca tggccggaaa tacagaataa tttggtaccg
    24661 cctgcattcg gaataccttt atcggcaaat gcctgtccac cgtcacggat aatgaatgga
    24721 acggaggaga acgtttcagt attgttgatg gtagtcggtt tgccgtacag gccgaacgaa
    24781 gcagggaatg gcggcttaaa gcgcggctgg ccttttttgc cttccaacga ttcgagcaat
    24841 gcggtttctt cgccgcagat gtatgcgccg tagccgtggt gggcgaagag ttcgaattca
    24901 aaatccgaac ccaaaatatt tttacccaaa aagcctgcgg cacgcgcctg ctccaaagcg
    24961 gcctcaaagc gttggtagcc ttcaaaaatt tcgccgtgaa tgtagttgta gcccgctttc
    25021 gcgcccatcg cgtaaccggc aataatcata ccttcaatca gggcatgcgg attgaacatg
    25081 atgatgtcgc ggtctttaaa cgtacctggt tcgccttcgt cggtgttgca aaccacatat
    25141 ttttcgcccg ggaaagaacg gggcataaag ctccatttca aaccggtcgg gaagcccgca
    25201 ccgccacgac cgcgcaagcc ggaggttttg acttcgtcaa tcacatcggt ttgcgagatg
    25261 ttttcggaca gaattttacg cagggcggta tagccgccgc gtttgacgta ttcgtccaat
    25321 gtccagcaat cgggattggc ggtatccact tggtcaaaaa tcacgcctga ttggtaaata
    25381 gccatttttg gtgtgcctgt ttgttttcgt atcggttgcg gccgctgttt cagacgacct
    25441 taagatgtct ttgtgtatcg gcttgtaacg tcgtctgaaa taaaatctag tttatcaaat
    25501 cgatattttc agacgacctt taatcgtttt tgttggatct cgaccacttg cttgtctgtc
    25561 gtcattcccg cgcaggcggg aatccatcct caatggtaaa caatgtctta ttaaattcag
    25621 aaaccgaatc ttaccggtgg attcccgcct gcgcgggaat gacggcattt cggtatttca
    25681 gtagggcgga ttcttaaatc cgacattttg cctttttacc cactctgtcg gatacaagta
    25741 tccgacctac atttaaatcg tcgtttcaga cgacctactc caactccgcc agtttcttct
    25801 caatcgcctc ttcagtcata aagccgcaca tgctgtggtt gttgaccagt atcaccggcg
    25861 cgtcgccgca cgcgcccata cattcgcctt cgacaagggt aaatttgccg tcgggcgtgg
    25921 tttcgccgta gccgataccg agtttttgtt tgaggtattc gccggtagcc ataccgccgc
    25981 gcagggcgca gggcaggttg gtgcagacgg tcagtttgta tttgccgaca ggctcaaggt
    26041 tgtacatatt gtagaaagta gcgacttcgt aggcttgtgc aggtgagatg ccgatatagt
    26101 ctgcaacaaa ggcgatggtt tcgggggcga gccagccttt ttcggtttgt gcaatacgca
    26161 atgcgcccat aatggcagag cggcgttggt cggcaggata ttttgccaac tcaatatcaa
    26221 tttgttttaa ggattctgcg gataacatta tcggtcaacc tccccgaata cgatgtcctg
    26281 cgtaccgatg atggcaacga cgtcggcgag catgtggcct tttgccattt cgtccatgcc
    26341 ttgcaggtgg gcgaagccag gtgcgcggat tttcaggcgg tagggtttgt tcgcgccgtc
    26401 tgaaatgatg taaacgccga actcgccttt cggatgttcg acagcggtgt aagtctcgcc
    26461 ctcgggaacg tgcataccct cggtaaagag tttgaaatgg tgaatcaggt cttccatacc
    26521 tgttttcatt tcggtacgtt tgggcggggc gaatttgtgg ttggtggtaa tgaccggacc
    26581 gggattgacg cgcagccagt caacgcattg tttgatgatg cgtacggatt ggcgcatttc
    26641 ttccatgcgg cagaggtagc ggtcgtagca gtcgccgttc acaccgacgg gaatgtcgaa
    26701 atccattttg tcgtacactt cgtaaggctg tgtcttacgc acatcccatt ccacgccgga
    26761 accgcgcaac atcacgccgg taaagccttt ttgcatggca cgctcgggag tgacaacgcc
    26821 gatgccgacg gtacgctgtt tccaaatacg gttgtcggtc aggagggttt cgagtgtatc
    26881 gatgtttttt gggaagcgtt cgcagaaggc gtcgataaag tcgagcatag tgccttcacg
    26941 ggattcgttg agctgcttca atactttggc gttgcggaat ttgctgctct cgtatttggg
    27001 catgaagtcg ggcaggtcgc ggtaaacgcc gccgggacgg aagtaggctg catgcatacg
    27061 tgcgccggac acggcttcgt acaagtccat cagctcttcg cggtcgcgga aggcgtagag
    27121 gatggcggtc atcgcgccga tgtcgaatgc gtgcgaaccg atgcccatca ggtgattgag
    27181 gatgcgcgtt acttcggcga acatcacgcg aatatactgg gcgcggatag gtacatcgat
    27241 accggcaagt ttttctactg ccaaacaata cgcctgctca ttgaccatca tggaaacgta
    27301 gtccaagcga tccatatagg gcagggcttg cagataggtt ttggtttcag ccagtttttc
    27361 agtaccgcgg tgcaggaggc cgatatgagg gtcggcacgg acgatggttt cgccctccaa
    27421 ctccaaaatc atacgcaata cgccgtgcgc cgcagggtgt tgcgggccga agttgatggt
    27481 gtagtttctt aatttattgg ccaccgtagt tctcctcacg gacgatacgc ggcgtgatct
    27541 cgcgcggctc aatggtaaca ggttggtaaa tcacgcgttt ttgctcttcg tcgtaacgca
    27601 tttccacata gccggaaatc gggaagtctt tgcggaacgg atgtccgaca aagccgtaat
    27661 cggtcaggat gcggcgcaag tccgggtggt tgttgaacat gatgccgtac atatcgaagg
    27721 cttcgcgttc gtaccaatcc gcgctgttgt aaatatcgac tacggattca actacaggga
    27781 agtcgtcgtc tgaaacccag acgcgtacgc ggatgcgttg attgttttta acggaaagca
    27841 gctgactgac gacggcaaag cgtttgccct gccatgcttc gtttttgtaa gtgctgtaat
    27901 cgacaccgca caagtcaacc agaagctcga aatgcaactc ttcatggtcg cgcagcgcgg
    27961 tcatgattga aacatagtgt tcgggcagac actcgacggt aatctcgccc aaagctgaaa
    28021 tgactttgct tgcctgatcg ccaagcacgc cgatgacggt ctcgtataag ttttgaatgc
    28081 ttgccatatc ttcctcccct tactcgtcac gcgcgatggt ggaagtgcgc ttgatttttt
    28141 gttggagctg aatcaggccg taaatcaggg cttccgcagt cggcggacaa cccggcacat
    28201 aaacatctac cggcacgacg cggtcggcac cgcgcacaac ggaataagaa tagtgataat
    28261 agccgccgcc gttggcacat gagcccatag acaataccca gcgcggctcg gcgagctggt
    28321 cgtacactcg gcgcagggcg ggcgccattt tattggtgag cgtacccgcc acaatcatca
    28381 ggtcggcctg acggggggac ggacggaaaa taatgccgaa acggtcaagg tcgtaacgcg
    28441 ccatacccgc gtgcatcatt tccacggcgc agcaggccaa gccgaaagta accggccaca
    28501 acgaaccggt acgcatatag ttcaacaccg tatccgcgct ggtggtgatg aaaccttttt
    28561 tcaaaacgcc ttctattccc attccagcgc accttttttc cattcgtaaa caaagcctac
    28621 cgtcagaacg acgataaaca ccagcataga ccagaagccg tatgcgccca aatctttgaa
    28681 caccaccgcc cacggcagca taaacgcaac ctccaaatca aacaggatga agaggatggc
    28741 gacgaggtaa tagcgcacat caaacttcat ccttgcgttt tcaaaggctt caaaaccgca
    28801 ttcgtaaggc gcgtcttttt cggcgtagtg gcgtttcggg cctaaaatcg tgccgagcag
    28861 gataaacagc acgccggccg cgaggccgat gaggataaag acaaagacgg gaaagtaagc
    28921 ggacaacatg gttacaccca aatccgttaa caaaatttct acaataattt cgtattttag
    28981 cgaatttcaa aaaccattaa aaggtaaata tcggcaaaac gcccaaaaaa acccaataaa
    29041 tacaatcatg ttatgataac gattcttatt tgattttata aggtcatata aatttttaca
    29101 ccgtaattcg tg
//
LOCUS       DAXHSJ010000005        26276 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000005 DAXHSJ010000000
VERSION     DAXHSJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.005"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(12..1307)
                     /locus_tag="ACFM8U_000179"
     CDS             complement(12..1307)
                     /locus_tag="ACFM8U_000179"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6333491.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVAGAAAEAGAQIFEQSPMTRIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1433..2320)
                     /locus_tag="ACFM8U_000180"
     CDS             complement(1433..2320)
                     /locus_tag="ACFM8U_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6333492.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2320..3285)
                     /locus_tag="ACFM8U_000181"
     CDS             complement(2320..3285)
                     /locus_tag="ACFM8U_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6333493.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3301..4425)
                     /gene="potA"
                     /locus_tag="ACFM8U_000182"
     CDS             complement(3301..4425)
                     /gene="potA"
                     /locus_tag="ACFM8U_000182"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC6333494.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4620..4727)
                     /locus_tag="ACFM8U_000183"
                     /pseudo
     CDS             complement(4620..4727)
                     /locus_tag="ACFM8U_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4806..5075)
                     /gene="rpsO"
                     /locus_tag="ACFM8U_000184"
     CDS             complement(4806..5075)
                     /gene="rpsO"
                     /locus_tag="ACFM8U_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC6333495.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5298..6233)
                     /gene="secF"
                     /locus_tag="ACFM8U_000185"
     CDS             complement(5298..6233)
                     /gene="secF"
                     /locus_tag="ACFM8U_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC6333496.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6237..8090)
                     /gene="secD"
                     /locus_tag="ACFM8U_000186"
     CDS             complement(6237..8090)
                     /gene="secD"
                     /locus_tag="ACFM8U_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC6333497.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDIIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8302..8568)
                     /gene="yajC"
                     /locus_tag="ACFM8U_000187"
     CDS             complement(8302..8568)
                     /gene="yajC"
                     /locus_tag="ACFM8U_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC6333498.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8696..9769
                     /locus_tag="ACFM8U_000188"
     CDS             8696..9769
                     /locus_tag="ACFM8U_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC6333499.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACTGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKIFRL
                     DNDTAISTGSLSAAHFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDKSG
                     RLNLHEADFGWLTHKIIQTASGCPGKVVSVLEGGYTLESLSKSAAEHIRVLAGLGKSE
                     TAAAYQAELDHNREHVPPDPNPA"
     gene            10024..11088
                     /locus_tag="ACFM8U_000189"
     CDS             10024..11088
                     /locus_tag="ACFM8U_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC6333500.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVVWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEDVDDIKVGQHVVVEPYIVHD
                     DVPTGPGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            11229..11552
                     /locus_tag="ACFM8U_000190"
     CDS             11229..11552
                     /locus_tag="ACFM8U_000190"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC6333501.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            11624..11947
                     /locus_tag="ACFM8U_000191"
     CDS             11624..11947
                     /locus_tag="ACFM8U_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC6333502.1"
                     /translation="MDNCIFCKIAAKDILAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            11994..12197
                     /gene="tatA"
                     /locus_tag="ACFM8U_000192"
     CDS             11994..12197
                     /gene="tatA"
                     /locus_tag="ACFM8U_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC6333503.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            12201..12887
                     /gene="tatB"
                     /locus_tag="ACFM8U_000193"
     CDS             12201..12887
                     /gene="tatB"
                     /locus_tag="ACFM8U_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC6333504.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTVSDGISDVMPSERSYASTETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            12901..13671
                     /gene="tatC"
                     /locus_tag="ACFM8U_000194"
     CDS             12901..13671
                     /gene="tatC"
                     /locus_tag="ACFM8U_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC6333505.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKISVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            13724..14329
                     /locus_tag="ACFM8U_000195"
     CDS             13724..14329
                     /locus_tag="ACFM8U_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC6333506.1"
                     /translation="MNALYLGSNSPRRMEILTQLGYQVVKLPADINETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPHSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGSIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            14345..14698
                     /locus_tag="ACFM8U_000196"
     CDS             14345..14698
                     /locus_tag="ACFM8U_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC6333507.1"
                     /translation="MNRQTAYLLASFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            14688..16502
                     /locus_tag="ACFM8U_000197"
     CDS             14688..16502
                     /locus_tag="ACFM8U_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC6333508.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDIKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            16692..17369
                     /gene="misR"
                     /locus_tag="ACFM8U_000198"
     CDS             16692..17369
                     /gene="misR"
                     /locus_tag="ACFM8U_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC6333509.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            17383..18789
                     /gene="misS"
                     /locus_tag="ACFM8U_000199"
     CDS             17383..18789
                     /gene="misS"
                     /locus_tag="ACFM8U_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC6333510.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SVDGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            18907..21333
                     /locus_tag="ACFM8U_000200"
     CDS             18907..21333
                     /locus_tag="ACFM8U_000200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6333511.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFDSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            21378..21623
                     /gene="rpsP"
                     /locus_tag="ACFM8U_000201"
     CDS             21378..21623
                     /gene="rpsP"
                     /locus_tag="ACFM8U_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC6333512.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            21639..22148
                     /gene="rimM"
                     /locus_tag="ACFM8U_000202"
     CDS             21639..22148
                     /gene="rimM"
                     /locus_tag="ACFM8U_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC6333513.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            22148..22897
                     /gene="trmD"
                     /locus_tag="ACFM8U_000203"
     CDS             22148..22897
                     /gene="trmD"
                     /locus_tag="ACFM8U_000203"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC6333514.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            22913..23278
                     /gene="rplS"
                     /locus_tag="ACFM8U_000204"
     CDS             22913..23278
                     /gene="rplS"
                     /locus_tag="ACFM8U_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC6333515.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            23599..24324
                     /locus_tag="ACFM8U_000205"
     CDS             23599..24324
                     /locus_tag="ACFM8U_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC6333516.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINTVQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCNGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            24359..25234
                     /locus_tag="ACFM8U_000206"
     CDS             24359..25234
                     /locus_tag="ACFM8U_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC6333517.1"
                     /translation="MNLYDLLLAPFTEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            25311..26231
                     /locus_tag="ACFM8U_000207"
     CDS             25311..26231
                     /locus_tag="ACFM8U_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFC6333518.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEESGHHHDHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALIKA
                     DPEGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEF
                     IAPQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKL
                     YSDALGSAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         6783 a         7885 c         6044 g         5564 t
ORIGIN      
        1 gcctgcgacg cttaacggct gtctttccac tgataatatt tcgagccgag gaaagaaccg
       61 agtttgcgca aaaacggttg caggataata ttcggctggc gcaaccgctc aaacggcttc
      121 agacggcatt cgtcccctaa aattgcttcg gcaaccgcca gacctgcaat gcctgttatc
      181 gccatcccgt gtccggaata accttgtgca taaaaaacat ttgcggccaa acgtccgaaa
      241 tgcgggacaa ggttggcggt aatgtcgcac tccccgcccc acgaatattc gattttgaca
      301 tcgtcaagct gcggaaaaac tttaagcata tcttggcgga caagctcggt catacgcgca
      361 ggattgtcga taaactcgtt atccttaccg ccgaaaagca gtctgccgtc cgcgctgagg
      421 cggtaataat ccaaaatatg gcggttgtcg catactgcca tattgttacg gataagccct
      481 tttgcgcgcg cccccaaggg ttcggtcgca ataataaagg tgctaacggc aatcgccttg
      541 cgttccaaag gctggaatat cgggttcaaa cctgcatagg tattgacggc atacaccaga
      601 tttttgcatt cgacgctgcc ttcgggcgtg taaaccagcc aaccgttttg atgcggttcg
      661 atgcgcgtca tcggggactg ctcgaaaatc tgcgcgccgg cttcggcagc ggcaccggca
      721 acacccaacg tgtaattgag cggatgcagg tgtccggata aggggtcgaa ttgtgcccct
      781 tggtacatat cgctgtcaag ctgctgtttc aactcggctt tatcccaaag ttgataatga
      841 ctcgcaccgt aatgccgttg ggcgtgttca tgccactgct gcaactcttc ccaatgctgc
      901 ggacggacgg caaccgtggc ataaccgcgc tgccaatcgc aatcaatggc atgtttgcgg
      961 acgcgttcgt ccaccagttc gaccgcctgc aaagactgtt gccaaaacca ttgcgcctgc
     1021 tccaagccga cctgtttttc aatttccccc ataccgcagg cgtaatcgct gataacctgc
     1081 ccgccactcc gtcccgacgc gccgaaaccg atacgcgcgg cttccaacac aaccgtttca
     1141 tgtccctgct ccgccaaggg caatgcagtg cacaaaccac tcaatccgcc gccgatgata
     1201 caggtatcgg ttttcagacg gcattgaagt ttcggataaa cagtatgagg attaaccgaa
     1261 ctgaaataat aagaaggcag atattcttga aaatcagggc gaatcattgt gtttgcttta
     1321 tcaggtgtat tttcggacgg aatgatacag gctgtcgggc catatcgtcc aaacagaaaa
     1381 tcggttgaag aaaacaggct gacccagtca tgcggtcagc ctgccttatt aattaatttg
     1441 ctttctcggc agccaatttt tcttggcggt aggcttctgc cgcttctcgg tcacgcttgg
     1501 ttgcctgcct catcatccaa taattgacga tgatgaccaa tgttccgatg atgccgatta
     1561 ggatggtcgc caagacattc atctgcggat cgagacccaa cttgattttg gagaaaatca
     1621 cctgcggcaa tgtggatgaa ccggggccgg agaggaatga ggtaatcacc aaatcatcca
     1681 aagacagggt aatgccgagc agaaagcctg aagcgatggc aggggcaatc aaaggcaaag
     1741 tgatgacaaa aaagattttc agcggacgcg cgcccaaatc cattgcggct tcttcgagcg
     1801 actggtcaag ctcaaccaga cgcgaacgga taacaacggt aatgtacgcc atacacagcg
     1861 tcgtatgtcc gaggaagatg gtgaaaaagc cacgatcgaa gtagagatgt tgtaaccatt
     1921 cgctgccctg caaaaatatc tgtacctgaa taatcagcag cagcatagac agaccggtaa
     1981 tcacgtcggg catcaccata ggtgcggaaa tcataccggc gaacaaggta ctgccgcgaa
     2041 aacgtttaat ccgcgccatc gcatagcctg ccagcgtgcc caaaacgacg gcagcaagcg
     2101 aagacacaac ggcaatccgc agcgacagcc aagcggcttc caagatggtg tcgttttcca
     2161 gcaatgcgcc gtaccacttg gtcgaaaagc cgccccaaac ggttaccagc ttggattcgt
     2221 taaacgaata gatgaccaaa acaaccagcg ggatatacag aaacgccagc gacagtgcca
     2281 acatcagttt caagaaccaa gataatttgg atttctgcat tatttggctc cttcttccaa
     2341 ttcgcggttt tcataatgct gaaacagggc aatcggcacg accagcagcg cgaccatcac
     2401 gacggcgacg gcggaagcca gcggccagtt gttttgatcg aagaacgcct gccacaagac
     2461 tttaccaatc atcaggtttt ccgaaccgcc gaccagctcg ggaatgacga actcgccgac
     2521 agcagggacg aaaaccagca tggagcctgc aataatgccg gttttcgaca aaggcagggt
     2581 aatcgtcaag aacgatttga ccggccccgc gcccaaatcg gaagccgctt caagcaggcg
     2641 gttgtcgagt ttcaccagtt gcgtgtatag cggcagaatc ataaacggca gataggcgta
     2701 aaccatcacc aaattgagcg aaaaggcatt gtagaacaaa tccaaaggct cgctgataat
     2761 acccatttta atcaacaggt tgtttacaat gccgttatgc ccgagcagac ccatccacgc
     2821 atagacgcgc aacaggaacg atgtccaaaa gggcagcata atggcaagca gcaaaccatt
     2881 gcggacagaa ggattggcac gagaaatcgc ataggcggtc ggataaccga ccaacagaca
     2941 aattaccgtc gtagtcagcg cagtcttaat tgaagaccaa taagtcatca gatagatatt
     3001 gctgttttca ctgtcgccga acgggttaag ggtatgccaa aaattttgga aaatatcggc
     3061 atagttttga taactgatgg cgatattcag acgacctaaa tcttcgtcga tgctggtcag
     3121 cggcgtaaac ggcggaatgg cgatttcttg ttcggcaaag ctgattttca gcacgatggc
     3181 gaacggaatc agaaacagca ccaaaagcca aatatacggt acggcaatca ccgcacgctg
     3241 ccccggacgg cggaacagtt tgtttttcag tttattaagg ttcattgcat tccccttaaa
     3301 tcaacggaac aacggagtcg gttggttttc cggccagctg atatagacgg tttcgtccca
     3361 agtcggcggt gtaatgttgc gcacatacca gtaaggggcg gggacttggc ttttgacgac
     3421 gcgcccgttg ccgagcttga tatggtaaat ggcgaagctg cccaaatagg cgatttcttt
     3481 taccgtgcct ttcgcccagt tgtagtcgcc caaatattcg ggtttttctt tatataaatc
     3541 aatatcctct ggtcgaatac taacccaaag gtcctgctcg ctcggaccac ccaaaccgtg
     3601 atcgatgcgg acgtggtttt ccaaaccttc gcattcgata acggcatagt cggcatgatc
     3661 ttcaatcacc acaccgtcaa agatgttggt ttcgccgata aactcggcag tgaagcggct
     3721 gttgggatag tcgtacacgt cgctgggtgt gccgacttgc tgcaactgac cgtcagacat
     3781 aatggcgatg cgggtcgcca tcgtcatcgc ctcttcttgg tcgtgcgtaa ccataataca
     3841 ggttacgccg acttgttcca gcgtattgac caactcaagc tgggtttgtt ggcgcagttt
     3901 tttgtccaat gcaccgaggg gctcatccag cagtagaatt ttcggacgtt ttgccagact
     3961 gcgtgccaaa gcaatgcgct gctgctgacc gccggacaat tggtgcggtt tgcgtttagc
     4021 aaatttggtc atctgaacca ggcggagcat ttcttcgacg cgcgcggcga tttcgccttt
     4081 aggcattttg tcctgtttca gaccgaaggc aatgttttgt tctacggtca tatgcggaaa
     4141 aagcgcgtaa ctttggaaca tcatattgat ggggcgatca tagggtgcaa gtttggtaat
     4201 atcctgacca tcaaggataa tttttccctg attgggactt tccatacccg ccagcatacg
     4261 cagcagtgta gattttccgc tgccggaact gcccaaaagg gcgaagattt cgtgttgata
     4321 aatgtccaag tcgatgttat cgacagcgta attgtcacca aactttttca ccaaaccttg
     4381 gattttgaga taaggtttgg ctgaagacgc agtggttgcg gtcataatgg caatactcca
     4441 ataaaaagac gagtaccggc aaaacggatt ttcgaatggg tgataaaaag ctgtttgatt
     4501 gctggcggga gttaaacgtt tgatgccgtc tgaaactctt gtaaagcgca cgggcagcat
     4561 gaaatggaac aagattccaa agaactttat attatattag tttatgcggt tttcgggcaa
     4621 tatagtggat taaatttaaa ccagtacagc gttgccttgc cttgccgtac tatttgtact
     4681 gtctgcggct tcgtcgcctt gtcctgattt aaatttaatc cactataaat atggatttga
     4741 gcttgtcgga aagcaacaga aaagaaaaca ccgcccattt ttctgggcgg tgtcggaaag
     4801 cgtaattatt tacgcagacc caagcgggta atcaacgcgc gatacgtatc gggctgggta
     4861 cggcgcaagt aggccagcag gcggcggcgt tggctgacca ttttcaacag gccgcgacgg
     4921 ctgtggtggt ctttggggtt ggctttgaag tggggggtca ggtcgttgat gcggaaagtc
     4981 aacagagcga cttgtacttc ggaagagccg gtgtcgcctt ctttgcgttg gaaatcttta
     5041 acgatttgtg ctttttgttc tacggtcagt gccataatgg aaactccaaa aatataagaa
     5101 tccccatagg gattcagaca agtttgccaa gcctgaagac aacggcaaac tccctatgcc
     5161 caagatagga caacgtggca ttatgacaca attcccgcat ttctgcacaa tattttaaga
     5221 ccgtggaagt aaccgttaaa aatgccgtct gaagcattgt ctgcttgaga cggcaatgtt
     5281 cagacggcat atgcgcttca aaccacgatt tcttctttct tcttcggttc tttaccgata
     5341 ttgtcgcggc tcaaaccgaa cattagcaga agcgggctgg caaccaatac ggaagaataa
     5401 atgccgaaca cgatgccgat ggtcaacgcc atagaaaagc cgtgcaaggc cgcaccgccg
     5461 aacaccagca tggatacgac catcgcctcg gtcgaaccgt gggtaatgat ggtgcggctc
     5521 atcgttgcgg taatcgcgtt gtcgatgact tccggcacgg catgtccgcg catcgccggc
     5581 ttgcggaagt tttcacggat acggtcgaag acgacgacgg attcgttcac agaatagccc
     5641 aatacggcaa ggatacccgc caagacggtc agcgaaaatt cccattggaa gaaggcaaag
     5701 cagccgagaa taatcacgat gtcgtgcata ttggcgataa tggcagatac ggcaaaacgc
     5761 cattcaaaac gcatcgacag gtaaataatg atgccgataa cgacaaaacc taaagccatc
     5821 aatccattac ttaccaattc ctcaccaact tgcgggccga taaattcgac ttggcgcaaa
     5881 gtaacgtcgg gactgtcttt tttcagcaaa tccataacct gattggacaa ctgtgcggaa
     5941 gtaacacctt ctttgttcgg caggcggatc atgatgtgtt tgttcgtacc caatgcctga
     6001 acctgtacat cacctatttt cagcgtatcg aggcgttcgc gcatcttatt gacatccgca
     6061 ccctgctgat attggacttc cattaccgtg ccgccggtaa attcgacaga gaaattcaga
     6121 cctctggtaa ccaaaaagaa tacggcggca ataaaggtaa ccaacgaaat aaaggtcgtc
     6181 agtttgccgt agctcataaa cggaatatcg cgtttgattt taaagagttc catagcttac
     6241 tccttgcctc ctgccatttc ggctttcggc ttccacaccg aaccgatgga aatattctgc
     6301 aatttgcgtc tgcgtccata ccacagattg accaacgcac ggaatacgac gacggacgaa
     6361 tacatcgaag tcagaatacc caaacagtgt acgaccgcaa aaccgcgtac cgggccggaa
     6421 ccgaatacca aaagcgcgat accggcaatc agcgaagtca ggttggaatc gacaatggtc
     6481 gcccatgcgt gttggaaacc gagattgatt gcctgctgcg gcggcacgcc ggcacgcaat
     6541 tcttcgcgga tacgttcgtt aatcaagacg ttggagtcga ttgccatacc caaagtcaac
     6601 gccagcgcgg ccatacccgg taacgtcaac gttgcctgca tggcagacaa aataccgatt
     6661 aggaacagta tgttggcact caatgcaatg gtagaaaaga aacccatcag acgatagtaa
     6721 accaccatga atgcagcaac gatggcaaaa ccccataaag tcgaatggaa gcctttttcg
     6781 atgttctcct tacccaaaga cggaccgatg gtacgttctt cgacaatctg catcggcgcg
     6841 gcaagagaac cggcacgcaa cagcaaagac gtatcattgg cttcggctgt cgtcatgctt
     6901 ccggaaattt ccacgcgtcc gccggtaatg gcagtacgga taaccggcgc ggttacaacc
     6961 tcggattttc cttggtcgat caaaaccatc gccatgcgtt tgccgacatt tgcggcagtc
     7021 agttcgccaa aaatgctgcc acccgcgctg tccaagctca gactgacagc aggtgcgccc
     7081 atttggtcga aactcggttg cgcatcgttg atgttgtcgc ccgtcagctc gacctgtttg
     7141 ctgattagca gagtttcggg acgatctccg ccgcttgaaa gcagctcata accgctcggc
     7201 acgttgcctt ccaatgcctc gcgcaacttg gcaggatcgt cctccaccat acgcaattcc
     7261 aaagtcgcgg tacggccgat gatgtctttt gccttggcag tatcctgaac gcccggaagc
     7321 tgcacgacga tacggtctgc acctgactgc tggatgacgg gttcggccac gcccaactcg
     7381 ttcacacggt tgtgcagggt agtgatgttc tgtttgaccg catcggaacg cactttattg
     7441 accgcctctt ccgaaagcgt caagacgata ttgctgccgt ctgaattcag cgttgcttca
     7501 ggaaacagct tgcgcaactg cggcagtgcc ttttgcacat cacctgcatc ctgcaaaggg
     7561 acggtcaggc tgtttccagc ctgacgcacc gtgccgctgc ggattttttc gcggcgcagt
     7621 tcgcggcgga tgtcgcccga ataacgttca aacgttttct gcatcgccgc tttcatatcg
     7681 acctgcatgg tgaaatgcac gccgccgcgc aagtccaaac ccaaaaacat cggattggct
     7741 ttgattttcg ccatccattc ggggctgtcc gccaacaggt tgagcgcggt aatataccct
     7801 tcgcccaaag tgttttcgat gatgtcgcgc gctttaagct gcgtttctgt gtctttgaaa
     7861 cgcactttca gtgaattgtc cacaacaaac atcccgtcgg tctgaatacc tgcgtttttc
     7921 agcgcggcat ccactttgga ttgagtctgt tcgttgatga tgatggcttg tcggttggtc
     7981 gatacctgca cggcgggtgt ttcgccgaat aggttgggca gcgaatacac tgcggcaacc
     8041 gcaatcgtga acacaatcag cagatatttc cataaaggat aacggttcat cattgttcct
     8101 taatggttgg aaccccaccc tttcggtggt gtcggaatcg ggctatttca gaagaggcaa
     8161 aaccccttcc cagccaggca agaccggaag gcggcatctt gaatatgccg ccctgcgtgt
     8221 cggaacatgg tcaagccttc ggttggaatt caaaacaaag tgccgcattc gggctttcca
     8281 gatgcggctt gtcggcacaa atcaatcgac ttttgcggca atcgcattgc gttctacttc
     8341 gacctcgatt tttgtaccct gtccgatatc cacggtaaaa aactgttcgc cgactctggt
     8401 taccttaccc ttgaaacctg ccgccaagac cactttgtcg ccgactttca aggcggcaag
     8461 cattgcctga tgcgctttga atttcttttg ctgcggacgc atgatcagga agtagaacac
     8521 caccataatc aacactaaag gagcaaattg tgcaacagct tgattcataa tttatccgtt
     8581 ctttctaata tggttgaaaa tcgagagggg tatataataa cataagaccg taaacaatat
     8641 atcgggtttg ccgtccgtac cgaccgtata ccgcagcctg cccgtccaca aacccatgtc
     8701 cttgacccgc ctaatcttga aattctatgc actattgcgc ctctttttgg gcaaaaacgc
     8761 ccgcaccgca tggatttcgc atcccgcctg cacagggcac gaacccggcg caaaccatcc
     8821 cgattcgccc gaccgcatcc tctgcatcga gcaggcattg cgccgcgccg gtatttggca
     8881 gcacctccaa accatagagg cggaagaaat cagcgatacg cgcctcgcgc ttgtccactc
     8941 gagcaaatat ctgaaccgtt tggaatcttg cctgccccaa aaaggcaaga ttttccgcct
     9001 ggataacgac actgcaatca gcacaggctc gctgtctgcc gcacactttg ccgccggttc
     9061 ggcagttcag gcagtcgaca tggtcatgaa ccgtaaagca tggcatgcct tttgcgccgc
     9121 ccgcccgccc ggacaccatg cgggcagcgg caaagccggc ggattctgcc tgctgaacaa
     9181 cgttgccgcc ggcgtcatgc atgccattgc cgaataccgc ctgaaacgca ttgccgtcat
     9241 cgatttcgat gtccactacg gcgacggtac ggcagaaata ttcaaagacg atccgcgcat
     9301 cctgtttttc aacctgtttg aaaccgacct ttttcctttc cccgaaaaca acgatatgcc
     9361 cgacggcggc aatatggtgc acctgccctt gccgccggga acgggcagcc gcacattccg
     9421 cgaagccgtc cgcagacagt ggctgccccg gcttgccgca ttcaaacccg aactggtgct
     9481 gctgtcggca ggattcgacg cacaccgtct ggacaaatcg ggcaggctca acctgcacga
     9541 ggcggatttc ggctggctga cacacaaaat tattcaaacg gcatcgggct gcccgggtaa
     9601 ggtcgtatcc gtgctggaag gcggttatac ccttgaatcc ttatcaaaat ccgccgccga
     9661 acacatccgc gtgctggcag gattgggaaa atccgaaacg gccgccgcct atcaggcgga
     9721 attggaccac aacagggaac acgtcccgcc cgatccgaac ccggcataag cacgccggac
     9781 cggccgtttt aaaacaaagg ctatgacggc ataggtatta tttaaatccg attcggtttg
     9841 caggcggtat cattatttga cctgaagcaa aacaagaaac ttttatctgc ttgaaatagg
     9901 ttttttgtag ccaaataaca cttgggcaga ttgaatccgg ctcttaccgg ccgccgtttc
     9961 catcccaacc gccgtccgac atttttcaga cggcattcct acttgaaaac aaggagcgtt
    10021 acaatgaaag cagcacgttt ttacgacaaa ggcgacatcc gcatcgaaga catccccgaa
    10081 ccgaccgtcg cccccggcac cgtcggcatc aatgttgtct ggtgcggcat ctgcggtacc
    10141 gacctgcacg aattcatgga aggcccgatt ttcattccgc cttgcggtca tccgcacccg
    10201 atttccggcg agtccgcacc cgtcacgatg ggacacgagt tctccggcgt ggtctatgcc
    10261 gtcggcgaag acgtggacga catcaaagtc ggccaacacg tcgtggtcga gccttacatc
    10321 gtacacgacg acgtaccgac cggcccgggc agcaactacc acctctctaa agacatgaac
    10381 ttcatcggct tgggcggctg cggaggtggc ttgtctgaaa aaatcgctgt caaacgccgt
    10441 tgggtgcatc cgatttccga caaaatcccg ttggatcaag ccgctttgat cgaaccgctg
    10501 tctgtcggac accacgccta tgtacgcagc ggcgcgaaag caggcgacgt cgcattggtc
    10561 ggcggcgcag gtccgatcgg tttgctgttg gctgccgtgt tgaaagccaa aggcatcaaa
    10621 gtcatcatca ccgagttgag taaagcacgc aaagacaaag cgcgcgaatc cggcgttgcc
    10681 gactacatcc tcgacccgtc cgaagtcgat gttgtcgaag aagtgaaaaa actgaccaac
    10741 ggcgaaggcg tggacgtggc attcgaatgt accagcgtga acaaagtgtt ggatactttg
    10801 gtcgaagcct gcaaacctgc cgccaatttg gttatcgtat ccatctggag ccaccctgca
    10861 accgtcaacg tccacagcgt cgtgatgaaa gaactggatg tgcgcggcac gattgcctac
    10921 tgtaacgacc acgccgaaac catcaaactg gtcgaagaag gcaaaatcaa cctcgagcct
    10981 ttcatcaccc agcgcatcaa gctggacgaa ctggtttccg aaggcttcga gcgtctaatc
    11041 cacaacaacg aatccgccgt taaaattatt gtgagtccaa acctgtaagc aaattacatt
    11101 tgcaacagca caaatgccgt ctgaaacgct cagacggcat tttccaacga agcgcaacag
    11161 gcgtattgcc cgagagcagc cgtttagcct atcattccga acaaacagaa acaaacagaa
    11221 ggaaaatcat gggagattcc gtactatccg ccatccaaca aaccatcacc cagcgaaaat
    11281 ctgccaatcc gtccgaatct tacgtcgcac agctcttgca taagggcgaa gacaaaatcc
    11341 taaaaaaagt gattgaagag gcgggcgaag tgttgatggc atccaaagac aaaaacccgt
    11401 cccacctggt ttacgaagtt gccgacttat ggtttcacac catgattctt ctgacacacc
    11461 acgacctgaa ggcggaagac gtattggacg aacttgcgcg ccgccaaggt ttgtcgggct
    11521 tggccgaaaa agccgctcgc acagaatctt gaatttatat taaaatccgc actttcccac
    11581 attcaatccg tctgaccgct tttcagacgg catcggagcc gttatggaca actgtatttt
    11641 ctgcaaaatc gccgccaaag acattctggc gcaaaccgtc tatgaagacg gcgaaatggt
    11701 ttgtttcaaa gacatcaacc ccgctgctcc ggttcatctg ctgctgattc ccaaagtcca
    11761 tttcgattcg ttggcacacg ccgcgcccga acatcagccc cttttgggaa aaatgatgct
    11821 gaaagttccc gaaatcgcca aagcggcagg actggcagac ggcttcaaaa ccctgatcaa
    11881 caccggaaaa ggcggcggac aagaggtctt ccacctgcat atacacatca tgggcacacc
    11941 cgtataaacc gttatttcac aatcaacccc taatacttac ttaaggatac atcatgggca
    12001 gtttttctct gacgcactgg attatcgtac tgattatcgt cgttttgata ttcggcacca
    12061 aaaaactgcg caacgtcggc aaagacctcg gcggtgcggt tcatgacttc aaacaggggc
    12121 tgaacgaagg cacagacggc aaagaagccc aaaaagacga tgtaatcgaa cacaaaaaag
    12181 acgaagacaa agcgtaattt atgtttgatt tcggtttggg cgagctggtt tttgtcggca
    12241 ttatcgccct gattgtcctc ggccccgaac gcctgcccga ggccgcccgc accgccggac
    12301 ggctcatcgg caggctgcaa cgctttgtcg gcagcgtcaa acaggaattt gacactcaaa
    12361 tcgaactgga agaactaagg aaggcaaagc aggaattcga agctgccgct gctcaggttc
    12421 gagacagcct caaagaaacc ggtacggata tgcagaacag tctgcacgat atttccgacg
    12481 gtctgaagcc ttgggaaaaa ctgcccgaac agcgcacgcc tgctgatttc ggtgtcgatg
    12541 aaaacggcaa tccccttccc gatgcggcaa acaccgtatc agacggcatt tccgacgtta
    12601 tgccgtccga acgttcctac gcttccaccg aaacccttgg ggacagcggg caaaccggca
    12661 gtacagccga acccgcggaa accgaccaag accgtgcatg gcgggaatac ctgactgctt
    12721 ctgccgccgc acccgtcgta cagaccgtcg aagtcagcta tatcgatacc gctgttgaaa
    12781 cccctgttcc gcataccact tcgctgcgta aacaggcaat aagccgcaaa cgcgatttgc
    12841 gtcccaaatc ccgcgccaaa cctaaattgc gcgtccgtaa atcataaaga gagcaatccg
    12901 gtgtccgaaa cacaaaacga acaacccgtc caaccgcttg tcgagcatct catcgagctg
    12961 cgccgccgcc tgatgtggac ggttgtcggc attctcgtct gctttttcgg cctaatgccg
    13021 tttgcccaac aactctatac ttttatcgcc gacccgctga tggcaaacct gcccaaagac
    13081 accagcatga ttgccaccga tgtcatcgca ccgtttttcg tgccggtcaa agttaccctg
    13141 atggcggcat ttttgatttc gctgccgcat acgctctacc aaatctgggc attcgtcgcg
    13201 cccgcactct accaaaacga aaaacgcctg attacgccgc tcgtcctctc cagcgtcagc
    13261 ctgtttttca tcggcatggc atttgcctac tttctggttt tccccgtcat tttcaaattc
    13321 cttgccagcg ttacccctgt cggtgtcaat atggcgacag acatcgacaa atacctatct
    13381 tttatcttag gcatgtttgt tgcgttcggc acaacgtttg aagttcccat cgtcgttatc
    13441 ctgttaacca aaatcagcgt ggtaacaacc ggacagctca aacgcgcccg cccctatgtg
    13501 attgtcggcg cgtttgtcat tgccgccatc atcacgccgc ccgatgtgat ttcacaaacc
    13561 ctgcttgcca ttccgctgat tctcttatac gaagcaggta tttggttcgg acgctttttc
    13621 acgccacgtt cagaacagga tggcgacata cagccgcctg caacaacctg acactatgcc
    13681 gtccgaacct ccgcctcata ccgcaacaga ttaaggaata cctttgaatg ccctctattt
    13741 aggttcaaac agcccgcgcc gaatggaaat cctgacacag ttgggctatc aggtcgtcaa
    13801 gctgcctgcc gacatcaatg aaacggtcag acagaacgaa gaccctgccc gttacgttca
    13861 aaggatggca gaagaaaaaa accgaaccgc cctgaccctc ttttgcgaaa ccaacggcac
    13921 aatgcccgat ttccccctga ttaccgccga cacctgcgtc gtttcagacg gcatcatatt
    13981 aggcaaaccc cactcccaag ccgaagcaat cgaattttta aaccgattgt ccggcaaaca
    14041 acataccgtc ctgactgccg tctgcattca ttatcgcggc aatgcggaaa accgcgtcca
    14101 aaccaaccgc gtcgttttca agcccctgag ttcggaagaa atttccgcct atgtccaaag
    14161 cggcgaaccg atggacaaag ccggtgccta cgccgtacaa ggcataggca gcatctttat
    14221 ccaatctatc gaaggcagct tcagcggcat tatggggctg cccgtttatg aaaccgtttc
    14281 gatgttgcag gatttgggat accgtacccc acccttcatc cgagcctaac ccgtaaagac
    14341 cgccgtgaac agacaaaccg cttacctcct tgcctctttc agcctgatcg cactgataat
    14401 cctgtccctt tcctgggaac tgtggattgc accgttgcgc ccgggcggct cgtggctcgc
    14461 gctcaaagcc ctccccctct gtctgccgct ttcaggcatc ttgaaaaaga aaatctatac
    14521 ttaccaatac agctccatgc tggttctgat ttactttgcc gaagccgtca tgcgcctgtt
    14581 cgacgcctat cccgcagaac agatttgcgc cgcgctttcc gcagtattca gcatcatctt
    14641 cttcatatcc tgcctgtcgt tcgtcaaaca atacaaggaa acaaacaatg cccgctgaaa
    14701 cgaccgtatc cggcgcgcac cccgccgcca aactgccgat ttacatcctg ccctgcttcc
    14761 tttggatagg catcgtcccc tttaccttcg cgctcaaact gaaaccgtcg cccgactttt
    14821 accacgatgc cgccgccgca gccggcctga ttgtcctgtt gttcctcacg gcaggaaaaa
    14881 aactgtttga tatcaaaatc cccgccatca gcttccttct gtttgcaatg gcggcgtttt
    14941 ggtatcttca ggcacgcctg atgaacctga tttaccccgg tatgaacgac atcgtctctt
    15001 ggattttcat cttgctcgcc gtcagcgcgt gggcctgccg gagcttggtc gcacacttcg
    15061 gacaagaacg catcgtgacc ctgtttgcct ggtcgctgct tatcggctcc ctgcttcaat
    15121 cctgcatcgt cgtcatccag tttgccggct gggaagacac ccctctgttt caaaacatca
    15181 tcgtttacag cgggcaaggc gtaatcggac acatcgggca gcgcaacaac ctcggacact
    15241 acctcatgtg gggcatactc gcctccgcct acctcaacgg acaacgaaaa atccccgccg
    15301 ccctcggcgt aatctgcctg attatgcaga ccgccgtttt aggtttggtc aactcgcgca
    15361 ccatcttgac ctacatagcc gccatcgccc tcatccttcc cttctggtat ttccgttcgg
    15421 acaaatccaa caggcggacg atgctcggca tagccgcagc cgtattcctt accgcgctgt
    15481 tccaattttc catgaacacc attctggaaa cctttactgg catccgctac gaaactgccg
    15541 tcgaacgcgt cgccaacggc ggtttcacag acttgccgcg ccaaatcgaa tggaataaag
    15601 cccttgccgc cttccagtcc gccccgatat tcgggcacgg ctggaacagt tttgcccaac
    15661 aaaccttcct catcaatgcc gaacagcaca acatatacga caacctcctc agcaacttgt
    15721 tcacccattc ccacaacatc gtcctccaac tccttgcaga gatgggaatc agcggcacgc
    15781 ttctggttgc cgcaaccctg ctgacgggca ttgccgggct gcttaaacgc cccctgaccc
    15841 ccgcatcgct tttcctaatc tgcacgcttg ccgtcagtat gtgccacagt atgctcgaat
    15901 atcctttgtg gtatgtctat ttcctcatcc ctttcggact gatgctcttc ctgtcccccg
    15961 cagaggcttc agacggcatc gccttcaaaa aagccgccaa tctcggcata ctgaccgcct
    16021 ccgccgccat attcgcagga ttgttgcact tggactggac atacacccgg ctggttaacg
    16081 ccttttcccc cgccactgac gacagtgcca aaaccctcaa ccggaaaatc aacgagttgc
    16141 gctatatttc cgcaaacagt ccgatgctgt ccttttatgc cgacttctcc ctcgtaaact
    16201 tcgccctgcc ggaatacccc gaaacccaga cttgggcgga agaagcaacc ctcaaatcac
    16261 taaaataccg cccccactcc gccacctacc gcatcgccct ctacctgatg cggcaaggca
    16321 aagttgcaga agcaaaacaa tggatgcggg cgacacagtc ctattacccc tacctgatgc
    16381 cccgatacgc cgacgaaatc cgcaaactgc ccgtatgggc gccgctgcta cccgaactgc
    16441 tcaaagactg caaagccttc gccgccgcgc ccggtcatcc ggaagcaaaa ccctgcaaat
    16501 gaccctcgcc ggcggatgcg gataccgccc gaaatgtaaa gctccatgca agacattgca
    16561 aaaaacagca aaccggtagg gaaatacgct atcaaaaaac attgcagccg tgttaagata
    16621 aaccgtcaaa caatcttttc acgccccgcc cgaaacaggg tcggggcata cccttacgaa
    16681 aaggaaacac catgagccgc gtattactcg tagatgacga tgccctgcta accgaactgc
    16741 tgaccgaata cctgagcgcc gaaggtctga acgtccgcag cgttcccgac ggggaagcag
    16801 gcgtacagga aatcctgagc gggcagtacg atgtagtcgt attggattcc atgatgccca
    16861 aaatgaacgg cttggatgtc ttgaaaaacg tacgcgcccg aagcaccgtc cccatcatca
    16921 tgctgaccgc caaaggcgac gacatcgacc gaatcatcgg cttggaaatg ggcgcggacg
    16981 actatgtccc caaaccctgc acaccacgcg aactcttggc acgcatcaat gccatcctgc
    17041 gccgcgcaca acacagcggc gaacagaaca acgcacccaa cagcatctcc gtcagcgatg
    17101 tcgtcctgta ccccgccaaa cgccaggcat ccgtcaaaga catgccgctc gaactgacca
    17161 gcaccgaatt caacctgctc gaagtcctga tgcgccatgc cggacaggta gtcagcaaag
    17221 aaaccctgtc cgtcgaagca ctcgaccgca agctggcaaa attcgaccgc agtatcgacg
    17281 tacacatctc cagcatccgc cacaagttgg gcgatgcctc tctgattcaa accgtacgcg
    17341 gcttgggcta cctgtttgtc aaaaactgaa ataaacagat aaatgaaact gttccaacgc
    17401 atcttcgcca cattttgcgc ggttatcgtc tgtgcaatct ttgtggcgag tttttctttc
    17461 tggctggtgc agaacaccct tgccgaaaac cagttcaacc aacgccgcac catcgaaacc
    17521 actttgatgg gcagcatcat ttccgcattc cgggcacgcg gggacgcggg cgcgcgcgaa
    17581 atcctgacgg aatggaaaga cagccccgtc tcatcgggcg tgtacgttat acagggcgac
    17641 gagaaaaaag atatcctgaa ccggtatatc gacagctata ccatcgaacg cgcccggctt
    17701 ttcgccgccg gacacccgca ttccaacctc gtccatatcg aatacgaccg cttcggcgaa
    17761 gaatacctgt tcttcaccaa agactgggac aaactccaag cccgccgcct gcccagcccc
    17821 ctgttgatcc ccggcctgcc gctcgccccg atttggcacg aactcatcat attgtccttc
    17881 atcatcatcg tcggactgct gatggcatat atcctcgccg gcaacattgc caaacccatc
    17941 agaatcttag gcaacggcat ggacagggtg gcaaacggag aacttgaaac ccgtatctcc
    18001 caacaggtcg acgaccgcga cgacgaattg tcccatcttg ccatccaatt cgacaaaatg
    18061 gtggaaaaac tcgaaaaact cgttgccaaa gaacgccacc tgctccatca cgtctcccat
    18121 gaaatgcgtt ctccccttgc gcgcatgcag gcaattgtcg gactgattca ggcgcagccc
    18181 caaaaacagg agcaatatct caaacggctg gaaggcgaac tgacccgcat ggatacgctg
    18241 gccggggaac tgttaaccct gtcccgtctc gaaacttcca atatggcttt ggaaaaagaa
    18301 agcctgaaac tcctgccctt cctgggcaac ctggtagaag acaatcaaag cattgcccag
    18361 aaaaacggac aaacggttac cctgtccgtc gacggaaaaa tccccgaaaa cacaaccatc
    18421 cttgccaacg aaagctacct gtaccgcgcc ttcgacaacg tcatccgcaa cgccgtcaac
    18481 tacagtcccg aaggcagcac catcctgatc aacatcggac aagaccacaa acactggata
    18541 atcgacgtta ccgacaacgg ccccggcgtg gacgaaatgc agctcccgca catcttcacc
    18601 gctttctacc gtgcagactc cagtgcgaac aaacccggaa caggactggg gcttgcattg
    18661 acccaacata ttattgaaca gcactgcgga aaaatcatcg ccgaaaacat caaaccgaac
    18721 ggtctgcgga tgcgcttcat cctgcccaag aaaaaaaccg gttccaaaac agaaaaaagt
    18781 gcgaactgac cataatgccg tctgaaccgg cttcagacgg cattgcacaa acagttaccc
    18841 caaaacaaac acacagcggg tgcatctcca acaatcacgc acataccgtc cgcaaaggaa
    18901 tatatcatgt cggcacaaac cgatccgggc tactttttca tgcccaacca cattattctg
    18961 ataggcgcga gcgaacgtcc gtacagcctg ggcgaacgcg tactcagcaa cctgctgagt
    19021 acgccctttc aaggaaaaat cacccccgta aacccgcgcc accacaccat agccgggctt
    19081 cccgcctaca ccagcctcaa caaaatcccc ggcaatgcag acctgattat tgccgttacc
    19141 ctacccgaca gttacgacac cctcttcaaa acctgccgca aaaagcagct ccgacacatc
    19201 atcctcatac aggactggga cagcctgtct gccgcagaac tgcacaccgc cgaaactgcc
    19261 atccgcaaac accacggcaa cggactcaac atcaccgcct gcaccaccgc aggcatccaa
    19321 ctgccctcac tcggactcaa catcagtacc caagacggat acgccgcagg ccataccgcc
    19381 atactgaccg gcaatgccgc cgtcagccgt caaatcgaca acatcctgaa caaactccgt
    19441 caaggcacat cccgccacat cagcctgcat cccggcatca gccccatcac atccgccgat
    19501 tggctcaacc gcttcggaca cagcctgcac accaaaaccg ccgtcctaca ccacaaccct
    19561 gaagaggatc agcgcaaact gttcagcgca atccgccaat ttacccgcca tacgccgctg
    19621 attctccaca tcacctgccg cacgacagaa accgaccgtg ccgtactgca ctgcctcgcc
    19681 cgacactgca acttcctcat cagtttcaac gccgacgacc tcgaagccgc actgcgcgcc
    19741 caactgtccg accttccacc cctgtcccga ctcgacatcc tgtccgacac gcctgccgaa
    19801 tggctgaacg cgcacgcgcc aaaaaccctc gccctccact ttcccaacct tccccatcac
    19861 atccgcaacg gacacctgac cggcacaccc acaccctcaa tctgccacga catcgcctca
    19921 cgtcagcttg cccgccccga cacccaagcc gtcctaacca tcctcagtcc ctccggacac
    19981 gaggattaca aaaaaacagc acgcgccctt atccgcctgt ccgaacagac cgccaaaccc
    20041 ctgctcgtca gcagcccctt ttcagacggc ataacacatt tcgacacccc cactcaggca
    20101 atccgcaccc tttcctaccg caacaccgcc gccgccctga aacaggcaca gctcgacatt
    20161 gcaccgccgc agccatgccg tctgaaaaca ccgcaacccc aaaacatcaa aaaagccctt
    20221 gcagcggcaa acccctccct gctcgccgaa gccctgcacc tcccccccta ccggcacacc
    20281 acccataacg ccgtacaatt ccaattcgac agccaccccc tctatggcga catcctgacc
    20341 gcacgctgca acggacaaac cactgccgta ctcccgccgt ttaccacgct cgacagccgc
    20401 caccttgccc gctttgccga actcgacggc acacaaaccc tcgaccagtt cctgcacaca
    20461 ctgaccatca ttcccgaata ccgccaacac attctcggca tcaccctcaa cctaaacggc
    20521 ggacaataca gcagcgattt catcttaaag acacccgaaa cacacgacac gcccaaacgc
    20581 aagaacacag gcaaagccgc ccaaaccctc gaacatgccg ccgcaaaaat gcagagtgcc
    20641 gccgcatacc tgaaacacaa aaacccgaca gccgccgaat ttctccgcca cacaagcgaa
    20701 gccgccgcag aactgctcgg cagcaaaacc gaaaccggag cagccgtacc caacgtactt
    20761 gccccctatc ccgcagcata ccccaaaaca ctgtccctaa gaaacaacac gaccgttacc
    20821 attaccccca ttttgcccga agacgcagaa gccaaacagc agttcgtccg cagcctcggt
    20881 cccgaagcac ggtacacacg cttcatgacc cacaccaacg aactgcccgc agccacgttg
    20941 gcacgcctgt gcaaccccga ttaccactgt gaagccgcat ggacggcaaa ggatgccgac
    21001 agcaacatcg tcgccgtcgt ccgccacagc cgcctgaatc gcaacgaatg cgaattcggc
    21061 atcacactgg cggaacatat gcgcggcagc ggactggcac agaaaatgat ggaactcatc
    21121 atccaaaccg ccgcacagca aggctaccgg actatgagtg ccgacattct caaaaccaat
    21181 acccccatga tcaaacttgc tgaaaaatca ggatttaccc tcaaggaatc ggacaccgaa
    21241 aaaaacctgt accgcgcata tctgaacctt gcggcagaca aaacaacaga aaaaacaaat
    21301 aaaaacttgc gcaccgacca caaaataacc taaaatcggc agtttccata tatccgattt
    21361 tctcaaaagg actcaaaatg gtagttatcc gtttggcacg cggcggctcg aaacaccgcc
    21421 ccttctacaa cgtcatcgtt actgactcac gcagccgccg cgacggccgc ttcatcgaac
    21481 gcgtaggctt ctacaacccc gtagccaacg aaaaacaaga gcgcgtccgc ctcaatgcag
    21541 accgcctgaa ccactggatt gcacaaggcg cgcaagtcag cgactccgtt gcaaaactga
    21601 ttaaagaaca aaaagccgcc taatccgcat ttgccgccat gacagacact caaaaccggg
    21661 tagccatggg ctacatcaaa ggcgtattcg gcataaaagg ctggttgaaa attgccgcca
    21721 acaccgaata ttccgacagc cttttggact accccgagtg gcatttggtc aaggacggca
    21781 aaaccatcag cgttaccctt gaagccggaa aagtcgtcaa cggcgaactc caagtcaaat
    21841 tcgaaggcat aaacgaccgc gacttggcat tctcattgcg cggttacacc atcgaaatac
    21901 cccgtgaagc attcgccccg acagaagaag acgaatacta ctggacagac ttggtcggca
    21961 tgaccgtcgt caacaaagac cataccgttt taggcaaggt aagcaacctg atggaaaccg
    22021 gcgcaaacga cgtattgatg attgacggag aacacgggca gattctgatt ccgttcgttt
    22081 cccaatatat cgaaaccgtc gataccggca gcaagattat tactgccgac tggggtttgg
    22141 actactgatg cttatccagg cagttaccat tttccccgaa atgttcgaca gcattacccg
    22201 ctacggcgta acgggacgcg cgaacagaca gggaatctgg cagtttgaag cagtcaatcc
    22261 ccgaaagttt gccgacaaca gattgggcta catcgacgac cgcccgttcg gcggcggccc
    22321 gggaatgatt atgatggctc cgccgcttca tgcagcaata gaacacgcca aagcacaatc
    22381 ttcccaagct gcaaaagtca tttacctcag cccccaagga aaaccgctga cacacccaaa
    22441 agcggcagaa ctggcagaac ttccgcatct gattctgctg tgcggacgct atgagggaat
    22501 agacgaaaga ctgctgcaaa ccagcgtcga tgaagaaatc agcatcggag acttcgttgt
    22561 ttccggcgga gagcttcccg ccatgatgct gatggatgcg gtattgaggc tcgtacccgg
    22621 cgtattgggc gatatgcagt ctgccgaaca agattcgttc tcaagcggca ttttggactg
    22681 cccccactac accaaaccct tagaatttca aggcatggct gttccggaag tattacgttc
    22741 cggcaatcat ggcttgatag cggaatggcg gttggaacaa tcgctgcgcc gcaccttgga
    22801 gcgcagaccc gatcttttgg aaaagcgcgt tttaatccca aaggaatccc gcctcttaga
    22861 aaccatccgg caagagcaac gggaaatcca atcataattt aggaaaaaaa caatgaacct
    22921 gattcaacag ctcgagcaag aagaaattgc ccgcctgaat aaagaaatcc ccgaattcgc
    22981 accgggcgac accgtagtcg tatccgtacg cgtcgtggaa ggtacccgca gccgtctgca
    23041 agcctacgaa ggcgtggtta ttgcccgtcg caaccgtggt ctgaacagca acttcatcgt
    23101 ccgcaaaatc tccagcggcg aaggtgttga acgtactttc caactgtact ctccgaccgt
    23161 cgaaaaaatc gaagtcaaac gccgtggcga cgtacgccgt gccaaactgt actacctgcg
    23221 cggcctgacc ggtaaagctg cacgcatcaa agaaaaactg cctgcacgca aaggttgatt
    23281 caaaccgttt tcccccaatg ccgtctgaac cttcagacgg catttcttat tgctgcgatg
    23341 ccggcaagtc aacgatatgc ctgcccctca tccaccttga ttaacaatca ctcccgccta
    23401 ttcaagcata atttacagac caaacgttat acagtatcat atcagccata taacgataaa
    23461 cgctttaccg ctttcccttt caaaaccgca aatcatcatg acaccttccc ttttactatc
    23521 aggattgacc ttccgcctca tccttgccct gattgccgta tcccttttat ggggcgttta
    23581 cttttgggca gtatccgcat gagcatcatt gtcgaaaacc tgaccgtcag ctaccgccgc
    23641 cgacctgccg tgcaccatgt ggacattact tttgaagaac atagtatgtg ggcggttttc
    23701 ggtcccaacg gcgcagggaa atccaccttt ctcaaatcct tgatgggatt gcagcctatc
    23761 gatacaggca gcatccggct ggacggattg acccgtcaga acattgccta ccttccccag
    23821 cagtccgaca tcgaccgctc gcagcctatg accgttttcg acttggcggc aatggggcta
    23881 tggtatgaaa tcggtttttt cagaggaata aataccgttc aaaaacaacg cgttcacgaa
    23941 gccttggagc gtgtaggaat gcggcagttt gcccgccgcc aaatcgccca cctctcaaac
    24001 ggacaatttc agcgtgtcct ttttgcccga atgctggttc aaaacgccaa attcctactt
    24061 ctggacgaac ccttcaatgc cgttgatgca cggacaacct acgagcttct cgacgtattg
    24121 caaaaatgtc attgcaacgg acacgctatc attgccgttt tgcacgatta cgaacaagtc
    24181 cgcgcctact ttcccaatac cctgctgctc gcccgagaaa aaattgcggc aggcgcaacc
    24241 gagaccattc tgacagaacc ctacctcgcc caagccaacg ccaaaatgca gcaacaggaa
    24301 agccccgact ggtgcgcctc ataaatactg cccgaaacca cgccccacac cagacaccat
    24361 gaatctctac gacctgctcc ttgccccctt tacagaattc gactttatgc gctacgccct
    24421 cgcatccgtc ttctgcctgt ccctcagtgc cgcacccgtc ggcgtattcc tcgtcatgcg
    24481 ccgtatgagc ctgataggcg acgcattgag ccacgccgtc ctgcccggtg ccgccgtcgg
    24541 ctacatgttt gccggcttga gcctgcccgc catgggtttg ggcggcgtag ccgcaggcat
    24601 gctgatggca ctgcttgccg gactcgtcag ccgcttcacc accctgaaag aagatgccaa
    24661 ctttgccgcc ttttatctca gcagcctcgc catcggtgta gtcctcgtca gcaaaaacgg
    24721 cagcagcgtc gatttgctcc acctcctttt cggctccgta cttgccgtcg atattcctgc
    24781 cctgcaactc atcgccgccg tatccaccct cacactgctt acccttgccg tcatctaccg
    24841 cccgctcgta ctcgaaagca tcgaccccct gttcctcaaa tccgtcggcg gcaaaggcgg
    24901 gctttggcac gtcctctttc tcgtcctggt cgtcatgaac ctcgtatccg gctttcaagc
    24961 cctcggcaca ctcatgtccg tcggactcat gatgctgcca gccattaccg cccgcctatg
    25021 ggcgaagcac atgggcgcac tcatcctcct atccgttctg acagccctgc tgtgcggctt
    25081 gagcggactg cttatttcct accacatcga aattccctcc ggtcccgcca tcatcctctg
    25141 ttgcagcgtc ctttatctct tttccgtcat actcggcaaa gaaggcggca ttctgaccaa
    25201 atggctcaaa aaccaccgcc accacaccac ctgaccccgc aagccgcttg aacaaacagg
    25261 cggcttgttt taccataaca gatgttacgt tatatcacag gagaacagtt atgaaacacc
    25321 tcaaactcac ccttattgcc gcattgctaa ccaccgccgc aactgctgcc cccctgcccg
    25381 tcgtaaccag cttcagcatt ttaggcgacg ttgccaagca aatcggcgga gagcgcgtat
    25441 ccatacaaag tttggtcgga gccaaccaag atacgcacgc ctatcatatg accagcggcg
    25501 acattaaaaa aatccgcagt gcaaaactcg ttctgctcaa cggcttagga cttgaagccg
    25561 ccgacatcca acgcgccgtc aaacagagca aagtatccta tgccgaagcg actaaaggca
    25621 tccaacccct caaagccgaa gaagaaagcg gacaccatca cgaccacgat catgaccatg
    25681 accacgaagg acaccaccac gaccacggcg aatatgaccc ccacgtctgg aacgaccccg
    25741 tccttatgtc cgcctatgcc caaaacgttg ccgaagccct gataaaggcc gaccccgaag
    25801 gcaaagttta ttatcaacaa cgcttgggca actaccaaat gcagctcaaa aaactgcaca
    25861 gcgacgcaca agccgcattt aatgccgtcc ctgccgccaa acgcaaagtc ctgaccgggc
    25921 acgatgcctt ttcctatatg ggcaaacgtt accatatcga attcatcgcc ccgcaaggcg
    25981 tgagcagcga agccgagcct tcagccaaac aagtcgccgc catcatccgg caaatcaaac
    26041 gcgaaggcat caaagccgtc tttaccgaaa acatcaaaga cacccgcatg gttgaccgca
    26101 tcgccaaaga gaccggtgtc aacgtcagcg gaaaactgta ttccgacgca ctcggcagcg
    26161 cgcccgccga cacctacatc ggaatgtacc gccacaacat caaagcctta accaacgcga
    26221 tgaagcaata aaaccgtttg attggtttga atttgaaaag gcaaaaggcc gtctga
//
LOCUS       DAXHSJ010000006        26149 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000006 DAXHSJ010000000
VERSION     DAXHSJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26149
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.006"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            360..650
                     /locus_tag="ACFM8U_000208"
     CDS             360..650
                     /locus_tag="ACFM8U_000208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333519.1"
                     /translation="MPSAQQANCPQKLFFMKNNPQVCTKGNPPYRHPFKTEIPMIFKI
                     KKSYPQKIPPINIIIVLLFKFYILFISLQGNARKSVKASFQKIKELRIVKQV"
     gene            702..2150
                     /gene="gnd"
                     /locus_tag="ACFM8U_000209"
     CDS             702..2150
                     /gene="gnd"
                     /locus_tag="ACFM8U_000209"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC6333520.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDESGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2213..3484
                     /gene="waaA"
                     /locus_tag="ACFM8U_000210"
     CDS             2213..3484
                     /gene="waaA"
                     /locus_tag="ACFM8U_000210"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC6333521.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRTGVPLFLANARLSEKSLN
                     GYLKVRSLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRQVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWRETVEKTLSYEEGGMQMQ
                     ARADGFIAQHRGAGARIAEAVWEAVCGHRGR"
     gene            3525..3974
                     /locus_tag="ACFM8U_000211"
     CDS             3525..3974
                     /locus_tag="ACFM8U_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC6333522.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLPILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4007..4975
                     /locus_tag="ACFM8U_000212"
     CDS             4007..4975
                     /locus_tag="ACFM8U_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC6333523.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKTA"
     gene            5051..5126
                     /locus_tag="ACFM8U_000213"
     tRNA            5051..5126
                     /locus_tag="ACFM8U_000213"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5084..5086,aa:Lys,seq:ttt)
     gene            5345..6598
                     /gene="murA"
                     /locus_tag="ACFM8U_000214"
     CDS             5345..6598
                     /gene="murA"
                     /locus_tag="ACFM8U_000214"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC6333524.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMVIEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6679..7857
                     /locus_tag="ACFM8U_000215"
     CDS             6679..7857
                     /locus_tag="ACFM8U_000215"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC6333525.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMIHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKDIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(7924..8172)
                     /locus_tag="ACFM8U_000216"
     CDS             complement(7924..8172)
                     /locus_tag="ACFM8U_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6333526.1"
                     /translation="MLTPEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNAKAQ"
     gene            complement(8263..9180)
                     /gene="ftsX"
                     /locus_tag="ACFM8U_000217"
     CDS             complement(8263..9180)
                     /gene="ftsX"
                     /locus_tag="ACFM8U_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC6333527.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYAGELG
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9177..9827)
                     /gene="ftsE"
                     /locus_tag="ACFM8U_000218"
     CDS             complement(9177..9827)
                     /gene="ftsE"
                     /locus_tag="ACFM8U_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC6333528.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(9824..10015)
                     /locus_tag="ACFM8U_000219"
     CDS             complement(9824..10015)
                     /locus_tag="ACFM8U_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333529.1"
                     /translation="MPSESASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            complement(10023..10505)
                     /locus_tag="ACFM8U_000220"
     CDS             complement(10023..10505)
                     /locus_tag="ACFM8U_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC6333530.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPTMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10629..10982
                     /gene="arsC"
                     /locus_tag="ACFM8U_000221"
     CDS             10629..10982
                     /gene="arsC"
                     /locus_tag="ACFM8U_000221"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC6333531.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            10979..11635
                     /locus_tag="ACFM8U_000222"
     CDS             10979..11635
                     /locus_tag="ACFM8U_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC6333532.1"
                     /translation="MKKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIIFTGGRSKKGYM
                     TEAEVGRRYALKQGIPPRNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGRDFFNWLFH
                     "
     gene            11828..13222
                     /gene="gltX"
                     /locus_tag="ACFM8U_000223"
     CDS             11828..13222
                     /gene="gltX"
                     /locus_tag="ACFM8U_000223"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC6333533.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFERYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLAQVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNTEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13243..13638
                     /locus_tag="ACFM8U_000224"
     CDS             13243..13638
                     /locus_tag="ACFM8U_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333534.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13642..14133
                     /locus_tag="ACFM8U_000225"
     CDS             13642..14133
                     /locus_tag="ACFM8U_000225"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6333535.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDFGTLLGSAAVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(14256..16850)
                     /gene="mutS"
                     /locus_tag="ACFM8U_000226"
     CDS             complement(14256..16850)
                     /gene="mutS"
                     /locus_tag="ACFM8U_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC6333536.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKSIADIERIAARIAVGNARPRDLAALRDSLFALSEIELSAECSSLLGTLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17294..18298
                     /gene="gap"
                     /locus_tag="ACFM8U_000227"
     CDS             17294..18298
                     /gene="gap"
                     /locus_tag="ACFM8U_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6333537.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18368..18487)
                     /locus_tag="ACFM8U_000228"
                     /pseudo
     CDS             complement(18368..18487)
                     /locus_tag="ACFM8U_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18556..19236)
                     /gene="tsaA"
                     /locus_tag="ACFM8U_000229"
     CDS             complement(18556..19236)
                     /gene="tsaA"
                     /locus_tag="ACFM8U_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC6333538.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEHFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQDIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            19351..19986
                     /locus_tag="ACFM8U_000230"
     CDS             19351..19986
                     /locus_tag="ACFM8U_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC6333539.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            20023..20991
                     /gene="waaC"
                     /locus_tag="ACFM8U_000231"
     CDS             20023..20991
                     /gene="waaC"
                     /locus_tag="ACFM8U_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC6333540.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            21218..21967
                     /locus_tag="ACFM8U_000232"
     CDS             21218..21967
                     /locus_tag="ACFM8U_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC6333541.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDAKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            21978..22913
                     /locus_tag="ACFM8U_000233"
     CDS             21978..22913
                     /locus_tag="ACFM8U_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC6333542.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVTAAAKLGKVDLLVAGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQANAEKLVLTFRATAFDAAAVQGGN
                     AEVINIETTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            22976..23587
                     /locus_tag="ACFM8U_000234"
     CDS             22976..23587
                     /locus_tag="ACFM8U_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC6333543.1"
                     /translation="MITTYKTITSPTQAGFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVSGLIHAYKEATAQALAIAEVIEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILEQDLQLDCRLTVSLSLANYEVCVAAWKNTRQIEVNTEKPFE"
     gene            complement(23639..23956)
                     /locus_tag="ACFM8U_000235"
     CDS             complement(23639..23956)
                     /locus_tag="ACFM8U_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC6333544.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24114..25385
                     /gene="purD"
                     /locus_tag="ACFM8U_000236"
     CDS             24114..25385
                     /gene="purD"
                     /locus_tag="ACFM8U_000236"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC6333545.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFSRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGRLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25419..26021
                     /locus_tag="ACFM8U_000237"
     CDS             25419..26021
                     /locus_tag="ACFM8U_000237"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6333546.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         6272 a         6026 c         7382 g         6469 t
ORIGIN      
        1 tttctgataa attcctaaaa cttaaaattt catcattccc actttcgtgg gaatgacggg
       61 atgtaggttc gtgggaatga cgcggtacag gtttccgtta cgtatcccca tccgaaactt
      121 tacttataca caagcaatcc aaagttatct gactgaaata ttgtgagatt tacccgcaaa
      181 tctcattttt tccaccactt atacacaaat tcggatgcgc actctgccga tatgcagaaa
      241 taagacattc gtgccgacac ctgaccattt ccacacgttc caaacacact tccggcttaa
      301 atctccacag tattcccgtt cttaaagaca tcggcagcca ttcggtaaag aaaagtagga
      361 tgccatctgc acaacaagca aactgtccac aaaaactatt cttcatgaaa aacaatccac
      421 aggtatgcac aaaagggaat ccgccttacc gacacccttt taaaacggaa atcccaatga
      481 tttttaaaat aaaaaaaagt tacccacaga aaattcctcc tatcaatata atcatcgttt
      541 tattatttaa attttatatt ttatttataa gccttcaggg aaacgcacgg aaaagcgtaa
      601 aggcatcttt tcaaaaaatt aaagagttaa ggattgttaa acaagtgtaa atcaggtaga
      661 gttgcggtcg ataaattaaa tctataacgg agcttcctgc aatgaaaggc gatattggcg
      721 taatcggttt ggcggttatg gggcaaaacc tgattttgaa tatgaacgat tgcggattta
      781 aggttgtcgc ctacaaccga acaatcggta aggtggacga atttttaaac ggcgcggcta
      841 agggaacgga tattgtcggc gcgtattccc tgcaagactt ggtcgataag ctggcaaaac
      901 cgagaaaaat catgatgatg gttcgcgcag gttcggtcgt cgacgacttt atcgaacaac
      961 tgctgcccct gttggacgag gaagacatca ttatcgacgg cggcaatgcc aattaccccg
     1021 acacgacacg gcggacacat taccttgccg aaaaagggat tttgtttgtc ggggcgggcg
     1081 tatccggcgg cgaagaaggc gcgcggcgcg ggccgtccat tatgccgggc ggggataagc
     1141 gcgcttggga agccgtcaaa ccgatttttc aggcgattgc cgccaaaacg ccgcagggcg
     1201 aaccgtgttg cgactgggtc ggcaaggacg gcgcggggca ttttgtcaaa atggtgcata
     1261 acggcatcga atacggcgat atgcagttga tttgcgaagc gtaccagttc atgaaagacg
     1321 gcttggggct gtcttacgac gaaatgcacc gcgtgtttgc cgagtggaac aaaaccgagc
     1381 tggattctta tctgattgaa atcacagcgg caattttggg ctataaggac gaaagcggcg
     1441 aaccgctggt cgagaaaatc ctcgatacgg cagggcaaaa aggcacgggc aaatggacgg
     1501 gcatcaatgc tttggatttg ggtattccgt tgacgctgat ttccgaagcg gtattcgccc
     1561 gctgcgtttc gtcgttcaaa gaacagcgcg tgcagaccgg caagctattc gcccgaaccg
     1621 ttacgcccgt cgaaggcggc aaacaagaat gggttgaggc gttgagacag gccctgcttg
     1681 catccaaaat catttcctac gcacaaggct ttatgctgat ccgcgaagcg ggcgaaagct
     1741 acggctggga tttggactac ggcaacactg cgctgctgtg gcgcgagggg tgcatcattc
     1801 gcagcgcatt cttaagcaat atccgcgatg cctatgaaaa caatcccgat ttggtgttct
     1861 tgggtgcgga tccgtatttc aaaaatattt tggaaaactg cctgccggcg tggcgcaagg
     1921 tggttgccaa ggcggtcgaa tgcggcatcc ctatgccatg catggcttcg gcgattacgt
     1981 tcttggacgg ctacacgacc gaacgtctgc ctgccaacct cttgcaggca cagcgcgact
     2041 acttcggcgc acacacttac gagcgcaccg acaaaccgcg cggcgagttt ttccatacca
     2101 actggacggg caagggcgga gataccgctt cgacaaccta cgatatttga ttccgttgcc
     2161 gaaatgccgt ctgaaccgtt cagacggcat ttgttttgca ggaaatagaa agatgttcca
     2221 atggctttat gatgtattgt ggctgcttgc gccgatatgg atacggcgtt atttggacaa
     2281 acgctccgga agtgccccgg catatcgggc gcatcgggac gagcgtttcg gcaagccgta
     2341 tccgaatccc gttaccggag cggtttggat acacgccgtt tcggtcggag aaacgcgtgc
     2401 cgcccagccc ttgatacgcg agttgcggca gcgttttccc gatgcaccgc tgctgatgac
     2461 gcagatgacc ccgacgggac gggaaaccgc gcaagttctg tttcccgatg cgcaatgccg
     2521 ctatcttccg tatgacaaaa aaacgtgggt acggcagttt ttgcgcgaac accgcccgat
     2581 gttcggcatt ttgatggaaa ccgaaatctg gcccaatctg atgaaggaat gccggcgcac
     2641 gggtgtgccg ctgtttttgg cgaatgcgcg gctgtcggaa aaatcgctga acggttatct
     2701 gaaagtccgc agcctgatcc gtcctgccgc cgcttcgctg acgggctgtc tggcacagac
     2761 ggaggcggat gcggcgcggt tggcgaaatt gggcgcggca tccgtgcagg tgtgcggcaa
     2821 taccaaatac gacctgatgc cgtcggaaca gatgaaaacg ctggcggggc agtttgaaaa
     2881 acgcatcgga gaccggcagg ttgccgtgtg cggtagcacg cgcgtttatc ggggcgaaga
     2941 cgaggcggaa aaactgctgg cggcgtggca acaatatcgc ggcgatgcgc tgctggtcgt
     3001 cgtgccgcgc catcccgagc attttcagac ggtatttgaa acggcaaaac gcttcgggtt
     3061 taaggttcag cggcgcagcg acggtttgcc ggtcgaacct gatacgcagg tgtggatagg
     3121 cgacagtatg ggcgagctgt atgcgtatta cttgtgcgcc gatgtcgctt ttgtcggcgg
     3181 cagtctggtc gattcgggct gtcagaacat tatcgaaccg ctttcctgcg gcgttccgac
     3241 gatattcggc ttttcaacct acaatttttc cgaagcctgc cggcacgcct tggcatcggg
     3301 ggcggcggtt cgggttgagt cggcggacgc gtggcgggaa accgttgaaa aaaccttatc
     3361 gtacgaggag ggcggaatgc agatgcaggc gcgcgcggac ggctttatcg cacaacatcg
     3421 cggagcaggc gcgagaatcg ccgaggcggt atgggaagcg gtatgcggac atcgggggcg
     3481 atagtgatac aatccgtatc cgagttttcc ggttggagca tcgtatgagt atttatgccg
     3541 tcgcgcacat cgtccacctg tattgcgcca ttgcctttgt cggcggcgtg ttttttgaag
     3601 tgctggtttt gtccgtcctg catacgggac gggtgtcgtg cgaggcgcgg cgcgaagtgg
     3661 aaaaggccat gtcttaccgc gccgtcaggg tgatgccgtt tgtggtcgga ctgctgttcg
     3721 ccagcggcgt cgtgatggcg gcaaaccgct accttcctat attgggcgaa ccgtttgcca
     3781 cttccttcgg tacgatgctg acgctgaaaa tcctgttggc gttcagcgtg ttggcgcact
     3841 tcgccatcgc cgtcgtcaaa atggcgcgtt ccacactgac ggtcggctgg tcgaaataca
     3901 tacacgccgt cgtctttacc catatgctgc tgattgtctt tttggcaaaa gcgatgtttt
     3961 atatcagctg gtaacacaac tacatttcaa atcaggagat tgaaccatga cagaatatcc
     4021 cggcatcggg tcgccgttgt tttacggcgt tttttttgcg gcagtgctgg tcatgattgc
     4081 cttggatatg ttttcgctga agaaaaacgg cagccataaa gtcggcgtca aagaagcctt
     4141 ggcatggagc ggcttgtggg ttgccgtatc ctgcctgttc gcgggctggc tgtatttcga
     4201 actcgccggc aatctcggct acggcgcggc agtcgcaaaa gaaaaagtat tggaattctt
     4261 taccggctac attttggaaa aatcgttggc agtcgacaat attttcgtgt tcctgatgat
     4321 attcggctac ttcaaagtcg caccgcagtt tcagcaccgc gtgctgctgt acggcgtatt
     4381 gggcgcattg gtattgcgca ccgtcatgat tttcgtcggc gcggcactgg ttcggcagtt
     4441 tgagtggatt ctgtatctgt ttggcgcgtt cctgctctat accggcatac acatgatgaa
     4501 gcccgaaggc gatgagaaag aggatttggc aaacagcagg ctgctgaatg ccgtcaagaa
     4561 agtcgttccg gttggcacgg aatttcacgg cgagaaattt tttaccgtcg aaaacggtaa
     4621 aaaaatcgcc acgccgctgt ttttggtgct agtcatgatt gaattgagcg atgtcgtgtt
     4681 tgccgtggac agcatccccg ccgtctttgc cgttaccacc gatccgttta tcgtgctgac
     4741 ttccaatatt ttcgctattt tgggcttgcg ggcgatgtat ttcctgctgg cggatgtggc
     4801 ggaacgcttt atcttcctga aatacggctt ggcattcgtg ttgggtttta tcggtgtgaa
     4861 aatgctggta atgcattggg tgcatatccc gatttccgtt tcgctgtcgg tcgtgttcgg
     4921 cgcattgggt gcatcgatac tgacctcgtt aatttatacg aaaaaaacag cctgataaat
     4981 aaaatgccgt atgagcgccg acaaaatggt tgacagcatc aggcttgatg tatagaattt
     5041 cgagcttatc gggtcgttag ctcagtcggt agagcagcgg acttttaatc cgttggtcga
     5101 gcgttcgaat cgctcacgac ccaccagata aaacaaaaaa gctgagtttt caaaactcgg
     5161 ctttttttgt cttccgacaa tccttgttcg gaaagcgcgg catttcgtca aattctttgg
     5221 tgcggcttcc gtatttttcc cgaaaatccc ttataattcc gcctctgcaa ccttcatcgg
     5281 gtgatgcttt atgcgttgtc ccgagtattg aaatattaca agtacccacc aggaaagaac
     5341 aaacgtggac aaactgaaaa tctccgcaaa cggcccgctc aacggggaaa taacggtctc
     5401 gggcgcgaaa aacgcggcat tgccgctgat gtgcgcgggt ttgctgacat cgggtacgtt
     5461 gcgcctgaaa aacgtcccta tgctggcaga tgtgaaaacc acgcaaaaac tgcttcaggg
     5521 gatgggcgcg cgcgttctga ccgacaatat cagcgaattt gaaattaacg gcggcacggt
     5581 aaacaatacc tgcgcgcctt acgaattggt acggacgatg cgggcctcga ttttggtgct
     5641 ggggcctact ttggcacgtt tcggcgaggc gcaagtcagc ctgccgggcg gctgcgccat
     5701 cggttcgcgc cccgtcgatc agcatttgaa aggcttggaa gcgatgggtg ctgagattgt
     5761 tatcgaacac ggttacgtca aagccaaagg caaactcaaa ggtacgcgcg tggcgatgga
     5821 tgtcgttacc gtcggcggca cggaaaacct gctgatggcg gcgacgctgg cggaaggtac
     5881 gacggttttg gaaaactgcg ccattgagcc tgaagtggtc gatttggcgg aatgcctggt
     5941 caaaatgggc gcgaaaatca gcggcatcgg tacgtccacg atggttatag aaggggtgga
     6001 cgagctgcac ggctgcgaac acagcgtcgt ccccgaccgg atcgaggcgg ggacgttcct
     6061 gtgcgcggtg gcgataaccg gtggcagggt ggttttgcgg aatgccgcgc cgaaaacgat
     6121 ggaagtggtg ttggacaaac tggttgaggc aggtgcggtg attgaggcgg gcgacgattg
     6181 gatcgccatc gatatgcggc agcgtccgaa ggcggtggac atccgcacgg tcgtccaccc
     6241 cggcttcccc acggatatgc aggcgcagtt tatggcattg aatgccgtgg cggagggaag
     6301 ctgccgcgtg gtggaaacga tttttgaaaa ccgctttatg cacgtccccg agttgaaccg
     6361 gatgggggcg aacatcacaa ccgagggtaa tacggcattt gtgcagggtg tggaacggct
     6421 ttccggcgca gtcgtcaagg cgacggattt gcgtgcgtcc gcaagcctcg ttatcgccgg
     6481 tttggcggcg cgcggcgaaa ccgtggtcga acagatttac cacttggatc gcggttatga
     6541 aaatattgaa aaaaaactcg gcagcgtcgg cgcgaaaatc gagcgcgtat ccggctgatc
     6601 gggttgcgat gccgtctgaa gccttcagac ggcatcaatt gtttctgtat cgcaaacact
     6661 gattgaaaag gactgattat ggcattttta aaactgaccg aacaaaacgt gcagggcaaa
     6721 accgtcctca tccgcgccga tatgaacgtg ccgttcaaag acggcaaaat cagcgacgac
     6781 acccgtatcc gcgcctcgct cgcgtccatc caatactgca tggacaacgg cgcatccgtt
     6841 atcgtgatga tccacttggg tcgcccgact gaaggcgagt tccaccccga agacgacgtt
     6901 gcgcccgttg ccgcgcattt gggcggtctg ttgggcaaag acgtgaaagt attgaacgac
     6961 tggcgcgaaa acaaaccggc tttgaacgcg ggcgatgtcg tgatgctgca aaacgtgcgt
     7021 atcaacaaag gcgagaagaa aaacgatttg gaactgggca aagcctacgc ttctttgtgc
     7081 gacgtgttcg tcaatgacgc gttcggcacc gcccaccgcg cccaagcctc gaccgaagcc
     7141 gtcgctcaag ccgcgcccgt tgcctgcgcc ggcgtattga tggcgggcga actcgacgct
     7201 ttgggcaaag ccctgaaaca gcccgcgcgc ccgatggttg ccatcgttgc cggcagcaaa
     7261 gtgtccacca agctgaccat cctcgaatcg ctggcggaca aagtcgacca actcatcgtc
     7321 ggcggcggta tcgccaacac cttcctgttg gcggaaggta aagacatcgg caaatctttg
     7381 gcggaacacg atttggtgga agaatccaaa aaaattatgg cgaaaatggc ggccaaagga
     7441 ggttctgtgc cgctgccgac cgatgtcgtc gttgccaaag cctttgccgc cgatgccgaa
     7501 gcggtggtaa aagacattgc cgacgttgcc gaagacgaga tgattttgga tatcggcccg
     7561 aaatctgccg ccgcgcttgc cgatttgctt aaagccgccg acacggtcgt ttggaacgga
     7621 ccggtcggcg tgttcgagtt tgaccagttc gcaggcggta cgaaagccct tgccgaagcc
     7681 attgcccaaa gcaaagcgtt ctccattgct ggcggcggcg acacgctggc ggcgattgcc
     7741 aaattcggcg ttaccgaaca aatcggctac atctccaccg gaggtggtgc gttccttgag
     7801 ttcttggaag gcaaagagct gcctgccgta gccgctttgg aaaaacgcgg cgcgtaagcg
     7861 gtttgatata aaaaatgccg tctgagacgt tgtgctgttt ctgacggcat ttttgtgtgg
     7921 cggttattgt gctttggcat tccattctgc cggtgtggcg gctactgaaa tggaaagtgc
     7981 gtgcagttcg ttgctttcca gttgggcttt aagcccgtct ttaatcaggc ggtggcgtgc
     8041 aaggcgtgcc ttgccttcaa attctgatga aacgatgacg gcgaaaaaat ggtgtccgtc
     8101 gccttctact tcgatatgtt cgcattttgc cacgccttca atcatggctt ttacttgttc
     8161 cggggtcagc atggtaggtt ccttgttgga aaaatgcgcc attatactgg caaattgaaa
     8221 tgccgtctga aaacgggttc ggacggcatt tttgacggtg ttttattttt tggctttgaa
     8281 gccgagcagg tgctgggtgg tggcaagcca cgcgccgaat acgcccaacg cgatgacgaa
     8341 gccgaacacc agcccgagtt cgccagcgta gaagaaccgc cagccgatat taagtccgta
     8401 gggtttgaaa atggcatcga ccaatgggcg cacggcagag agcagccaac cgcaaagccc
     8461 caagctgacg gcggcggaaa ggatgctctg ccacatggct tgataaagga aggggcggcg
     8521 gataaacgac gcgggcgcgc ccaagagttt ggtgatttcg atttcttctt ttcggctgag
     8581 gatttgcagt cggatggtgt tgtgcgcgac aaggacgaac gccatcccca gcgtcaggga
     8641 aagaaaccat aagattttac ggatgaattc gttgatttgg tacagtgtct gtacccattc
     8701 cgtatccatg ttggcggatt cgaccatagg cagtttggta atgtctcggt agattgcctg
     8761 catttgggcg ggcgtggttg ccgggtcggg ggtaacgata aagacatccg gcagggggtt
     8821 gccgtcaagc atggaaatca gattttggtc gagattggac tgtaattctt ccaaaccgtc
     8881 ttctttgccg atgaagcgga tgttgtcgag ccgtttgtcg cgcgccagca ggctgcggac
     8941 ggtatcgctg tcgctttgtg cggcggcggt ttccatatag acggtgattt gcggcgactc
     9001 attgagtttg cccaacacgc tttgcccgct ttggatgccc aaatacataa acagcggcag
     9061 ggtcatggcg acggcgagca ttatgagggt aagcagtgtg ccgaaaggtt ggcgcagaag
     9121 ctgtttgagc gcggtgcgcg cggattcgac gtgcagcgag aggtagtgga tgatgctcat
     9181 gcgagtcgtc ctttcgagag gcgcaggatg cggtgtccgt agtccgccat cagggtttcg
     9241 tcatgtgcgg cgacgatgac ggttgttccc gcttcgtgga aggttttgaa caattccata
     9301 atatcgagcg cgtaggcgcg gtcgaggttg gcggagggtt cgtcggcaat cagcaggccg
     9361 ggctggtgaa cgacggcgcg ggcgatgcac aggcgttgtt gttcaccgcc ggagagggtt
     9421 acgggatcgt ccaattctcg ccctttcaag ccgacttttt caatggcgat gcgggcgcgt
     9481 tcttcggctt tgcgcggcgg ataaccgata atccgaagcg gcaggatgac gttttgcagg
     9541 acgttgcggt cgtagaggat tttgtggtct tggaacacga tgccgatgtg ttggcgcata
     9601 aagccgattt ggttgtcgga caatgtgccg aggtcctgcc cgttaaacag gattttgccc
     9661 ctgctcggct tggtgatgcc cgaaatcagc ttgaggacgg tggatttgcc cgaaccggag
     9721 tgtcctgcga taaatatcat ttcgcctttg ttgatttgga agctgacgtt tttcagggct
     9781 tcaaaaccgc cgggataggt tttggaaact tgttcgaaac ggatcatagg aggtcctgta
     9841 aggaatgggc ggcatatcgt ttcgggcggt tgcgtccgca gggatatgcc gtctgaacaa
     9901 taggtaagac agtgccgatt ataaagattc cgcctgtttt tagatagtgg ataagtttgg
     9961 cggataccga aggtcgggcg ggaaaaatgc cgtctgaagc ggattcagac ggcatccggc
    10021 gtttaacggc attttgaatg ggcgagtttg acggcttcgg tcaggctttt ttcgttcact
    10081 tccccggtaa tggtctgcct gtatccgcat ttcggtgctt cgacgacggt aaagggcagt
    10141 acgccgacag tgtttccgta ggttttcata aagtttcggc tgttcgcccc ggtgtaacgc
    10201 caaatcgggt aggaaacagg agtttgtttg aggaagttgc cgatattgtc ggatgtgtcg
    10261 agcgcgatgc cgaccatatc gacgctgcct tttttctgcg ctttgtacca tttggacatg
    10321 gtaggcatct cttttcggca cggaccgcac caggttgccc aaaggttgac gatgcgtacg
    10381 ggggctttga gtgattgcag gctttgtggg gtgttgtctt tccatccggc cagttcgtct
    10441 gccgaggcgg tatgtgcggc aaatgtggcg gcaagggtga ttgcggcaag attcaagtat
    10501 ttcatcgttt tgtcctttgt atgtcgattc gggtattgta aaccgtttgg tacggttgta
    10561 ggcgggatat tgagtaaaac cctgtgtaag ccgataataa ggggattgtc gagaagtaag
    10621 gaaatgtcat gtctgaaatc aaaattttcc acaacccccg ttgcagcaaa tcgcgtgccg
    10681 ccttgtcctt attggaagaa cgcggcattg ctgccgaagt ggtcaaatat ttggatacgc
    10741 cgcccgactt gtccgaattg aaggatattt tcaacaaatt gggcttggag tcggcgcgcg
    10801 ggatgatgcg tgtgaaagat gatttgtaca aggaattggg tttggacaac cctgatttgg
    10861 acaatgacgc gctgctgcgt gccatcgccg atcatcccgc cctgttggag cgtccgattg
    10921 ttttggcgaa cggtaaggcg gctgtaggca ggcctttgga aaatatcgaa gcggtattgt
    10981 gaaaaaacgg ctgttttgca gccgcaacgg cttgcgctat tacctgttgg gcggtttctg
    11041 tttgagtgtt tttccgcttt tgctggtgtt tgcctcatcc gtttgggcgg tttatcggac
    11101 gggcggtcag gttttgccgc cgtatgtccg ggcggatgcc gcgctggtgt tgggcgccgc
    11161 cgcgtgggac aaacgccctt ccccggtttt tcgggaacgc atcaaccacg ccatcgcgct
    11221 ttatcagagc cgcagggtcg gcaaaatcat ttttaccggc ggcaggagta aaaagggtta
    11281 tatgacggag gcggaagtcg ggcggagata cgcgctcaag caaggaattc cgccgcgcaa
    11341 catcctgttt gaaaacactt cgcgcaacac ttatgaaaac ctgaacaaca tccgtccggt
    11401 tttgcgtgcc aacggtattg ccagcgtggt gattgtcagc gatccctacc atttggcgcg
    11461 ggcggcggaa atggcggagg atttgggtgt ccgcgtttat atgtctgcca ccccgaccac
    11521 gcggtttgat gcgggaaata agaaaaaaat ctttatgctg caagagggat atgcgttatc
    11581 cctctaccgt ttggaaaaat gggggcggga ttttttcaat tggctgtttc attgaacctt
    11641 tttttcggac gcaatcccga aggaacccgg tcggcggcgc gtgatacact gttccgcgtg
    11701 ccgaagcatt gaaaacaagg aaaatgccgt ctgaaactta tccgcaggca gggttcggac
    11761 ggcattttta atgcccaagc acggtaaaaa cagtaaaata agccgtttta aagattctgg
    11821 agccatcatg accgtcaaaa cccgtttcgc ccccagcccc accggctacc tgcacattgg
    11881 cggcgttcgc accgccttgt tttcatgggc gtttgcccgc catcacaaag gcgagttcct
    11941 attgcgtatc gaagacaccg acttggcgcg ttccaccgcc gaatccgtca acatcatcct
    12001 cgacggcatg aaatgggtcg gtctcgatta cgataacgcc gacaacgtgg tgtaccaaac
    12061 ccgccgcttc gaacgctata aagaagtcat cgccgaactc ttggcaaaag gcgatgccta
    12121 ctactgctat tgcagcaaag aagaattgga agcgatgcgc gagaaagccg aaaaggaagg
    12181 cacggcgact tacgaccgcc gctggcggcc ggaagcaggc aaaaccctgc ccgaaatccc
    12241 tgccggcgtg caacccgtcg tccgcttcaa aacgcctttg gacggcgtaa ccaagtgggc
    12301 ggacctggtc aaaggcgaaa tttccatccc caacgaagcc ctcgacgacc tgattattgc
    12361 acgcgccgac ggcacaccga cctacaactt ctgcgttgtc gtagacgact acgacatggg
    12421 cgttacccac gttatccgtg gtgacgacca tgtgaacaac accccgaaac aaatcaacat
    12481 attaaaagcc atcggcgcta ccctgcctga atacgggcac ctgccgatga ttctcaacga
    12541 acaaggcaaa aaaatctcca aacgcagcgg cgataccgtt gccattaccg atttcggcgc
    12601 aatgggcatc ctgcccgaag ccatgctcaa ctatctggca cgtttgggct gggcgcacgg
    12661 cgacgacgag ttctttacga tggaacaatt catcgaatgg tttgatttga aagacgtttc
    12721 cccgtcccca agccgtatgg atttgaaaaa actctattgg atcaacggcg aacacatcaa
    12781 aatcacgcct aacggcaaac tcgccgaact cgtcaaaccg cgtctcgctc tgcgcgatat
    12841 ccatgaaact gaaaaacctg cgttggaaga cgtgttggca caggtcaaag accgcgccca
    12901 agatttgaac gccttggcag acgaatgtct gtatttctac aaaaaacaag tccctgccga
    12961 agccgatgtt gccaaacact gggatgacga agcggcggca cgcatgctgc gctttgccga
    13021 acgcctcgag ggtttggaag actggaatac cgaagccatc cacgacctgt tcaaaccttt
    13081 ctgtgacgaa gaaggcatta aaatgggcaa actcggcatg cctttgcgcc tcgctgtctg
    13141 cggcacggcg aagaccccaa gcgtcgatgc cgtattggca ttaatcggca aagaagaagt
    13201 gttgaaacgc atccgcgctt aagttatcca caggcgtggg cgatgccgtc tgatgtcgga
    13261 atgcggcttc agacagcatt caaacggaag ttgaacatga aaaaaatatt gtattttctg
    13321 atgtttgttt tttctacaag cgtatgggcc ggggatgcgg aagacaatct gctcagcatc
    13381 caatccggct atcgtgcctt actgcaaaaa caaaacaatc tggacggaaa aatcatcggg
    13441 atgcagtcgg atttggaaga tgcgcgccgg cgtttgcaga cggctcaagc agacatcgcc
    13501 cgtttggaag cggaaattcc tgcagcaatg gcgcaaaaag cccggcaggc tgaagagctg
    13561 aggcaaatcg gagtgcgttt ggaccatgct tggaatgcgg tttacggcgc agggggaacg
    13621 aaagcgtcgg ggaattgacc gatgaattcc ctctttgtag acaatactgt tttcattaca
    13681 cggctgaaag ccgggcatat cggcaggttg gttcaggcgt tgtttgagga gtggcacgga
    13741 tttgaaccgt ggtcttctgt ggataagatt catgcctatt acggcaggtg tttgaaggat
    13801 gacgaactgc cgctggcatt tgcggctgtg gatgatttcg gaaccctgtt gggttcggct
    13861 gcggtcaagc ggcatgatat ggaaagtttt ccacagtatg aatattggtt gggtgatgtc
    13921 tttgttttac ctgaatatcg cggaaaaggc attggcagga ggctggtcgc ccactgcata
    13981 ggcgcagcgc gttcgctggg gataaagttc ttgtatcttt atacgcctga tgtgcaaata
    14041 ttttatgaat cattcggctg ggcggttgtc gggcgacatt tccataacgg tgaatgggtt
    14101 acggttatgc gtttggatat ggataaggtt taagccgaat ggacaaatgc cgtctgagct
    14161 gttcggacgg catttttatg tttggaaact tcttgtcaga taatgtttat ccacagattt
    14221 ttccatatgg tttgatgctg ttccgacaac ggaaattaag atacggattt gcacaaatct
    14281 ttcagacggt acagttctga caatgcttcg cgcggggtca ggctgtcggg atcgagtttt
    14341 tccaaggctg ctgccaatat accttcaaaa tgtttttcct ctgctttatc cacaaagttg
    14401 cccacattcg gttcatctcc tttttcagac ggcatggtac tgaaaatatc cagttgggga
    14461 cggttcgcgg cggcttggtt ttccagtccg ttcaaatgct tttgggcgga tttcaatgcg
    14521 cgtacaggca ggccggcgag tttggcgacg gcaatgccgt agcttttacc ggcgggaccc
    14581 ggttggattt ggtgcaggaa aacgatgtcc tgtccctgtt cgagcgcgga aaggtgcata
    14641 ttgacggcgg cggcgtgggc ttcgggcagg taggtcagct cgaaatagtg ggtagcaaac
    14701 aggctgaagg atttgttttt ttgcagcagg tgttcggcaa cggcgtgcgc gagggcgagg
    14761 ccgtcgaaag tggaagtacc acgtccgact tcgtccatta aaacaaggct ttgttcggtg
    14821 gcgtgatgca ggatgtaggc ggtttcgctc atttcgacca tgaaagtgga gcggttggag
    14881 gcgaggtcgt ccgatgcgcc gatgcgggtg aagatttgat cgatgggccc gattgtggcg
    14941 gcatcggcag gcacaaaaca gccggtgtgt gccaataaaa caatcagcgc gacttggcgc
    15001 atgtaggtgg atttgccgcc catattgggg ccggtgagca gcatgaggcg gtgtttgtgg
    15061 tcaaggtcgg tgtggttggc ggtgaagtgg cgtacctgct gttcgacaac gggatggcgg
    15121 ccgttttcga tgtggataac cggatagtcg gcaaactcgg ggcggacgaa gttccgctct
    15181 tttgccaagg ctgaaaatgt ggacaacacg tccagcgcgg cggcggcttt ggcggctttt
    15241 tgaagctgcg gcaatgccgt ctgaaggttt ttcaatacgc cgtcaaagag ttgtttttct
    15301 aaggcgaggg cttgctcttg agcagtcagc actttgtctt caaaggcttt caactccggc
    15361 gtgatgaagc gttcggcgtt tttgagggtt tgccggcgtt ggtagtcggc aggtgcttgt
    15421 tcggcttggg ttttggacaa ttcaatgtaa aagccgtgaa cgcggttgaa ctcgactttg
    15481 agtgtggaca aaccggtacg ttcgcgttcc ttggcttcca aatccagcaa aaattcgtcg
    15541 ccatggtttt gaatgcggcg caattcgtcc agttcgggat gaaaaccgtg gttgatgaca
    15601 ttgccgtctt tcagccagac ggaaggttcg ggcaaaatgg cttggcggag ctgttcggct
    15661 gtggataggt tttccgggaa aacggctttg agggttccta agagactgct gcactcggcg
    15721 gacaattcga tttcggacag ggcaaacagg ctgtcgcgca gggcggcgag gtcgcgcggg
    15781 cgggcgttac ccacggcaat acgggcggcg atgcgttcga tgtcggcaat gcttttcaga
    15841 cggcactgga ggggtttgta ttggctttcc agcgcggcaa cggcttcttg gcgcgctcgg
    15901 atgtgggcgc ggttgcgtaa agggtggtgc agccagagag ccaagaggcg gctgcccata
    15961 tgggtagcgc aaaggtcgag cgtggacatc agggtcggcg attttttgcc ggagagggtt
    16021 tgcgtgattt cgagattgcg gcgcgtggcg gcatccatac cgatatattg gctgtcggtt
    16081 tcgagcgaca ggccgtccaa atgttgcggc atcaggtttt gcgtcagacg gatatagttc
    16141 aacagtgcac ctgccgcgcc aatcgcaacg gcgtgttctt tgccgtccaa accgaagccg
    16201 cgcaaatcct ggcagccgaa atattccgtc agcagttttt cccccgcgtc ggcggcaaac
    16261 tgccacgcgt tcaggcgcgt aacacccgat gccgtctgaa gttgcggtgc gtttttactg
    16321 tccggcaaca gaatttccgc cgcctgcagg cgcgccagtt cgtcgtccaa tttatccaca
    16381 gttgtcagct tggttttgaa ttcgccgctt tgcagcgatg cccacgccaa accgatgtat
    16441 tttttgtcgg gggacacggc aacgatgcgg ttggtttcct tgtcttccag caatgcggaa
    16501 tcggtcagcg tgccgggcgt tacgatgcgc acgactttgc gctccaccgg tcctttgccc
    16561 gcgccgactt cgccgacctg ttcgcaaatc gccacgcttt tgcccaactt gaccaggcgc
    16621 gccagatatt gttcggcggc gtgaaacggc acgcctgcca ttttgaccgg ctcgccatcc
    16681 acctgtccgc gcgtggtcag ggtaatatcc aaaagttttg ccgcttctac cgcatcgtcg
    16741 aaaaacatct cgtaaaaatc acccatgcgg taaaacacca gtttgtcggt atgttgcgct
    16801 ttgatgccga ggtattgctg catcattggg gaaacggcgg atttggacat ggggctgcct
    16861 tatcattagg aaaacggtat tgtagcaaaa ggttttgtct tcgacggaat gagactgacc
    16921 gtaagcctgt caataaggtt gtggagacaa gtaggaaaac agcaaagtta tccacggaga
    16981 tattattaag tcaaaaaaca tccacgttta tctgtcctat acggcaagcc ttaaactcaa
    17041 acttatttga cgaaaaggta atgaaaatat tggatatttg caacttatac acaagcagat
    17101 ccgcaatcat caaaatcatc attaaaattt taaattcaaa tattgatggt aaaagttttg
    17161 gaaatctgtg caaacagtaa aattcatcta tatcaaataa gcatcaagta gttagatgta
    17221 atagtggaat gtaactttat ttcagaatac aatacagata ttgaaatttc gtttcacagc
    17281 ttggagtata tacatgagca ttaaagtagc gattaacggt ttcggccgca tcggccgcct
    17341 cgcattgcgt caaattgaaa aagcccacgg catcgaagtc gtcgctgtca acgacctgac
    17401 ccctgccgaa atgctgctgc accttttcaa atacgacagc acgcaaggcc gtttccaagg
    17461 tactgccgaa ttaaaagacg atgccatcgt ggtaaacggc aaagaaatca aagttttcgc
    17521 caatccgaat cccgaagaat tgccttgggg cgagttgggt gtggatgtcg tcctcgaatg
    17581 taccggcttc tttaccaata aaaccaaagc cgaagcacac atcagggccg gcgcgcgcaa
    17641 agtcgtgatt tccgcacccg gcggcaatga tgtgaaaacc gtcgtatatg gcgtaaacca
    17701 agacattttg gacggcagcg aaaccgttat ctccgctgct tcctgcacca ccaactgcct
    17761 tgccccgatg gcggcagtcc tgcaaaagga attcggcgta gtcgaaggcc tgatgaccac
    17821 catccacgcc tacaccggcg accaaaacac ccttgacgcg ccgcaccgca aaggcgattt
    17881 gcgccgcgcc cgcgccgccg cgctcaacat cgtgcccaac agcaccggtg ccgccaaagc
    17941 catcggtctg gttatccccg aattgaacgg caaactcgac ggctccgccc aacgcgtccc
    18001 tgttgccacc ggctcgctga ccgaattggt ttccgtcctc gaacgccctg taaccaaaga
    18061 agaaatcaac gccgcgatga aagcagccgc cagcgaatct tacggctaca acgaagatca
    18121 aatcgtttct tccgacgttg tcggcatcga atacggctcg ctcttcgatg cgacccaaac
    18181 ccgcgtgatg accgtgggcg gcaaacaatt ggtgaaaacc gttgcctggt acgacaacga
    18241 aatgtcctat acctgccaac tcgtccgcac tttggaatac tttgcaggca aaatctaagg
    18301 cttgaacaaa cctgtggata agtaaatgcc gtctgaacac attgcgttca gacggcattg
    18361 tttatctgta tagtggatta aatttaaacc agtacggcgt tccctcgcct tgccgtacta
    18421 tttgtactgt ctgcggcttc gttgccttgt cctgatttaa atttaatccg ctataacttg
    18481 atattgcggc ttatggcgaa caaacaacag ccgtttgttc aactgctatg cggcaaaacc
    18541 ggatttttgc ccgatttaaa gcggggttgg ggaaagatca ataacggttg cacggttttc
    18601 ctcgatttga aatctgactt cgtaatccgc aatattcatc acataaatcc gttcgggaat
    18661 atcctgatag gctgggcgcg gatcttgggc aatgctttgg ctgataaggt ttttggtgtt
    18721 tgcagataaa ttttccgcgc cgatgttttc ctgccaaacg acttccaact ctacgggttt
    18781 gccgctgacg aaaccagatg cggcatcggg tttggattcg acaaagggga tataaggttt
    18841 gatgtccaca atcggtgtgc cgtccagcag gtctgcaccg ctgcaataga ggcggacggg
    18901 tttgccggtt tcgatgcgtt cgagtttcag gagcgagagt ccgagatggt tggggcggtg
    18961 ggggctgcgc gtggcgaaca cgcccatttt ttgtttgccg ccgagccgtg gtgggcgcac
    19021 catttgcgcc cagccttcat ccaatacgcc gtgaaaaata aaacttatcc acacatagtc
    19081 gaagtgttcc agtccgcgta cgctgtctgc ggtaaattcg ggattcagct cgatgcatac
    19141 ttctgcggca gagaccaaac cgggctggcg tgcgatgccg aatttctgtt tgtagggcga
    19201 tcgggcggtg gcgataggga cgatggtgta agtcatgagg attgtggata agtttttcgg
    19261 aaaaacctta tcttatcaca gcctgtttta ccgcttttgg gcagtaattt gttataatac
    19321 agggcatttt aattgcgttg aaatgatgag atgattgttt ccattgatgt tgatgcacaa
    19381 aaaactttta cgcctttgtg tcctaaagag ctgcctgtaa acgaggggca tttgattgtc
    19441 gaagaattga atgcccaagc cgctttggcg gatttgcgcg tgatgacgaa agatgcgcat
    19501 catgcggcag caaaatggct tgtggataac cctgttgata tgttgaagcc gacaggtttg
    19561 cccgatgcgg atttgacttg ggtggctcat gcgatggttg gtacgcgcgg ctatgaattg
    19621 ttggacgggc tgccttgtgc caaagaatac gattattgcg tttggaaagg tgttgatcct
    19681 gaattgcatc cttacggcgc gtgttttcac gatattgagg aaaaactaag cacagggctg
    19741 attgaatggc tgcgttgtca aaatacggat acggttatta tcggtgggtt agctacggat
    19801 tattgtgtta aaacaacggt tttgcagtta ctcaaaggcg gtcgttggca ggttgtcgtc
    19861 aatgaagcgg cttgtcgggg tattgcgccg gaaaccatcg aaacagcatg gcaggaaatg
    19921 cattctgccg gggcaatcat cttaaaaaac gctgatgaaa taaataaata tattaataat
    19981 caataagtta aaattgtttc acgtgaaacc cccttcctaa atatgaaaat tttgcttgtc
    20041 cgcttgtcta gtatgggcga tttaattcac actttgcccg caatcgaaga tttggcgcga
    20101 caatgtcctg atgtagaact gcattggcta tgtgaggctg gatttgcaga tattgcgcgc
    20161 ctgcatccgt ttgtaaaaaa aatccatgtg atgaaatggc ggcaatggcg caaacatctc
    20221 tttcgggctg aaacttggcg ggaaatgggt catctgaaac agactttgcg gcaggaagta
    20281 tttgatttcg tattggacag tcaaggtctg attaaaagcg cgtgtttcgc taaaatggca
    20341 aaatccccga tttgtggttt ggataaaaac agtgcgcgcg agggatgggc tgctttggcg
    20401 tatgtaaaaa catacgctgt accgaaggga aaaaatgccg tttggcgcaa ccgtgaactg
    20461 tttgcccaag tatttgggta tgtaatgccg gaaacgcagg tatttggctt gactgttcct
    20521 gaagcaggcc gtctgaaaaa tttagcgcag ccgtattatg cggctttgca tgcgaccagt
    20581 cgggacagta agttatggcc tgtggaaaac tggcggtcgt tgctgcaaaa gttgaatgaa
    20641 gaacagcaat gcaatattta cctgccttgg ggaaatgaag atgaaaaaac gcgtgccaaa
    20701 caaattgcag acggactgcc gtttgctatt gtgtgcgaca aaataaattt attgcaggca
    20761 gcgtatctgc tgaaatacgc ggtcggaatt gtcggcgtgg ataccggttt gctgcatttg
    20821 gcaaatgcct tggaaaaacc tgtggtcggc atttataccg ataccgatcc gattaaaaca
    20881 ggcgttcagg tttcgccaat tgcaaaaaat ttgggcaata tcgggcagat tccgaccgca
    20941 gatttggttt atcaagcgct gatggattgt gttgcagcag ataaaggcta aagggtcgtc
    21001 taaaactgat atggcggcat gatattttcc aatttattag atacaggaat gtatatccgc
    21061 tagtctgagt aaatgcttta aaaatggtat aatcggcaag cattatccga aagatttttg
    21121 aatgaattcg tttttaagga aaacggattt ggttttatag ggaagaaggt ttggtttcgc
    21181 tggatggtga aagcagcgtt ctttttagga gggttcaatg aaagcactgg tcgcagtaaa
    21241 gcgcgtagtg gactacaacg tcaaagttcg tgtaaaagcc gatggttcgg atgtggatat
    21301 cggcaatgtc aaaatgtcga tgaatccgtt tgacgaaatc gctgtggaag aagccgtccg
    21361 tttgaaagaa gccggaaaag taagcgagat cgtagcggtt tctttgggcg aaaagaaatg
    21421 cgaagaaacg ttgcgtacag ctttggcgat gggtgccgac cgtgcaattc atgttgaaac
    21481 cgatgcgaaa ttggagccgc tggcagttgc caagttgctg aaagccgttg cggacaaaga
    21541 aaatccgcaa attttctttt tgggtaaaca agcgattgat gacgatgcta atcaagtggc
    21601 gcaaatgctg gcagctttgt tgaatgcggc gcaaggtacg tttgcttcaa aagtacaaat
    21661 tgaaggtgat gaagtacaga ttgtgcgcga aattgatggc ggcgaagaaa ccatagcatt
    21721 gaaattgccc gctgttatca gtgcagattt gcgtttgaac gagccgcgct tcgtcaaact
    21781 ccccaatatt atggcggcga agaaaaaacc tttggaaaaa ctggctcctg ccgatttggt
    21841 tgctgacatt tcacctcgtc tgaaaacggt gaaattcgcc gagcctaaag cgcgtcaggc
    21901 aggcgtaaaa gtagcaagcg ttgccgaatt agttgaaaaa ttgaaaaacg aagccaaagt
    21961 gatttgagga gactgaaatg agcgtattga ttattgccga acacgacaac aaacagttga
    22021 atcctgccac tttgcatgct gttaccgctg ccgcaaaact aggcaaagtc gatttattgg
    22081 ttgccggaaa cggcgcatcg tccgtcgtgg agttcgcgaa gcaagtagcg ggagtggaaa
    22141 aagttttggt tgcagatgct gcccattatg ctgaaggttt ggctgaagag ttggctccgc
    22201 tggttgtcaa attggcggcg gattaccgct atgttgcagc aacggcaacc acattcggta
    22261 aaaacctttt gccccgcgta gccgccttat tagacgtacc gcaaatttct gatttgaccg
    22321 agattgtaga taacacgact tttgtacgtc cgatttatgc aggtaatgca tttgaaaccg
    22381 tgcaagccaa tgcggaaaaa ttggtgctga ccttccgtgc gacggctttt gacgcagcgg
    22441 cggtgcaagg tggtaatgct gaggtaatta atattgaaac aacccctgcc caaaacctga
    22501 gtcgttttgt taaccgtcag ctttcccatt ccgatcgtcc cgaactgact caggcaaaag
    22561 tgattgtttc tggtggtcgt gcgttgggta gtgcggaaaa atttaatgaa gtgctgacac
    22621 cgttggcgga tgttttaggt gcggcaatcg gtgcatcccg tgcagcagtt gatgccgaat
    22681 atgcgccaaa cgatgctcaa gtcggacaaa ccggtaaagt agttgcgccg caactctatt
    22741 ttgcaatcgg tatttcaggt gcgattcaac acgttgccgg tatgcaagac agtaaggtga
    22801 ttgttgcaat caataaagat gctgatgcgc cgattttcaa tgtagccgat tacggattgg
    22861 ttggcgattt atttgaaatc gttccacaat taacagaagc attgaaaaat taatgtttca
    22921 cgtgaaactt tcagacgact ttgttattaa tgaggttgtc tgaaaacttt tcaacatgat
    22981 tacgacttat aaaaccatta cttccccgac gcaggctggg tttaaagata aaggcagccg
    23041 ttttatcgca tttgcctatc cgattcggac attggctgat gtgaaaaaat acctcgatcc
    23101 gttaaaggaa gagcaccata aagcacgaca ctggtgttat gcctatcgtt tgggcgtgga
    23161 tggtgtgcaa tttcgtgcca acgatgatgg agagccatca ggaagtgctg gacgaccaat
    23221 tttgggacag attgattcgg tgggtatcac cgatgttttg gtcgtggtcg tccgctattt
    23281 cggtggtact ttattgggcg tttcaggtct gatacatgcg tacaaagagg cgacggctca
    23341 ggcgttggcg attgcggaag tgattgaaaa gaatattgaa aaaaccgttt ggctgaaatg
    23401 tgaatatcca tttttgaatg aagcgatacg catcgccaaa caatatcagg cggatatatt
    23461 ggagcaggat ttgcagttgg attgccgatt gacggtaagt ttgtcgttgg caaattatga
    23521 agtctgtgtt gcggcttgga agaatacacg gcagattgaa gtaaatacgg aaaagccttt
    23581 tgaatgaaag gtcgtctgaa atgctttgtg atggtatttc agacgacctc ttcgctgact
    23641 aatcttcttg tgtatatttg tctaaggcat cgacgctgga gcgcaaatat aaccaagcca
    23701 gttgatggaa ttcgcccagc gcgttccata gcgcatcttc actcataatg ctgtattcgc
    23761 cttccgtacg caaatccagt tctaccccgt cttcaatagc ctgatgacac gcctgatact
    23821 cgtgggtata gaaattgtcc atgtcgcgaa ttaaagtgac gacatatttc caacggcgga
    23881 tgtcttcttc cagttgcggt agtaaatgac accattcgcg gtatttgctt tggatttcat
    23941 caatacgttt ttgcatggtt gcttgtcctt ttttaattta tggataagtt gaataggttg
    24001 cagagtggaa tgaagcggtc ttttgtttga ttctactaaa atttcttcag atgggataaa
    24061 agtccctttg tatcgagttt tcgttaaaag tacatatttg tcaaggagat ggtatgaaac
    24121 tgctggttat cggtaatggc ggtcgcgaac acgcgctggc ttggaaattg gcgcagtcgc
    24181 ctaaagtgga aacagtattt gttgcgcctg gtaatgccgg tacagcgatt gaacccaagt
    24241 tgcaaaacat cgacttgact gcgcatcagg atttgattga attcagtcgt aaagaaaata
    24301 ttgtttttac cgtcgtcggc cctgaagcgc ctttggcggc aggtgttgtg gatgatttcc
    24361 gtgccgcagg actaaaaata ttcggtccga cacaatatgc ggcgcagttg gaaagttcca
    24421 aagatttcgc caaagcattt atggcgaaat acaacattcc gaccgcgcaa tatcaaacct
    24481 ttgaaaacgc cgatgccgca catgattacg tcaatcagaa aggtgcgcct atcgttatca
    24541 aagccgatgg tttggcggca ggtaaaggcg tgattgtggc gatgactttg gatgaagcgc
    24601 atgctgcgat tgatgatatg ctgctggaca acaaaatggg caatgccggc gcgcgtgttg
    24661 tgattgaaga tttcctgcaa ggcgaagaag cgagttttat cgtcatggtt gatggcaata
    24721 atgtactgcc tatggcaacc agccaagacc acaagcgtct tttagacggc gacaaaggtc
    24781 tgaatacggg cggtatgggt gcgtacagcc ctgcgcctgt ggtaacgcct gttgtgtacg
    24841 agcgcgcgat gaatgaaatt attttgccga ccgtagcagg gatgaaagcg gaggggcatg
    24901 agttcaccgg tttcctgtac gcaggtttga tgattgatca aagcggtgca ccctatacga
    24961 ttgagtttaa ctgccgtttc ggcgatcctg aaacccagcc gattatgagc cgactgaaca
    25021 gcgacttatc ggatttggtt gaagcggcaa tagacggcag actcgatagc gtaaccgcgg
    25081 aatggagccc gcaaactgcc gtgggcgtgg tactggcggc gcaaaattac cccgaaacgc
    25141 ccaaaaaagg cgatgttatt tccggtctgg atgctgccaa ccaagtaggc aaagttttcc
    25201 atgcaggtac aacggcaaac gagaaaggtg acgtattgac caacggcgga cgcgtattgt
    25261 gtgttgtggg attgggcgac aatgtggcgc aagcgaaagc taaggcatat ggcgcgttgg
    25321 aaaaaatcag tttcgacggg atgcaatacc gtaaagacat tgccgataaa gcgattaacc
    25381 gttaactaga aagaggatga tgccgtctga agccaagtat gactgatgtg cttcagacgg
    25441 tattttatat tatgttattt ccgagaatta tcgcagcatc agcgcaacgt aagttaagcg
    25501 tttatttgaa aaaaggcggt ttggtcgctt atccgaccga atcttgttat ggcttggggt
    25561 gtcttccgac attagccaaa gcactcggta agttggcgca tttgaaaaaa agaccgcaac
    25621 acaagggtat gattgtcatc gggaatcaat ttgaacaatt gcagccttta cttcaaatgc
    25681 cgtctgaaaa tcttcaggat atgcttagaa aagaatggcc tgcccctaaa acgttcctat
    25741 tatccgccaa atcctgtgtc ttacctgagt tgcgcgggaa acagcgcagc aagttggcgg
    25801 tcagggttcc cgcccatgta ggtgcgcgcc gcttgtgtca ggctttgcaa acgcctttgg
    25861 tctcgacttc gtgcaaccgc gcaggcaaac gcgcgtgtcg gacggagagg gaagtcagaa
    25921 ggcaattcgg acgcgatgtg tggattgttg gtgggagaat cggcaggcag aaatcgccga
    25981 gtcagattat tgacggggaa acaggtaaac gtttgcgtta gatatgggta cttgaatgaa
    26041 aacttttttg catacacaaa gaaaaaattt tggggctgta ctagattagc cctaaatccc
    26101 acaccaatcc tgtaggattt ttagctgccg ggacagtgtg ccgaagtta
//
LOCUS       DAXHSJ010000007        25306 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000007 DAXHSJ010000000
VERSION     DAXHSJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25306
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.007"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            75..4550
                     /gene="mspA"
                     /locus_tag="ACFM8U_000238"
                     /gene_synonym="ausI"
     CDS             75..4550
                     /gene="mspA"
                     /locus_tag="ACFM8U_000238"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HFC6333547.1"
                     /translation="MRFTHTIPFYSVLSTLGLFAVSPAYSSIVRSDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLSGIPMPDFRVSNRQTAIATLVHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAVAGDSGSPLFAFDKHENRWVLAGVLGTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGGSTLQGLNEHITLPIANPSLAPQN
                     DSRHMPSADAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPILSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMENSTSWQFIGNNRQQAAEQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNGPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPVPTSHGMPTA
                     PKTGNFSDMTGEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYDLSGIDLGFIQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPAT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPLKLEGDSRGAFQI
                     HVKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNP
                     LKEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARI
                     DSQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSADICRQVAKAADTNDLTLFETELD
                     TYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNL
                     AELISRSANTAVSEQAAYNTGRQQAERRIDRHLTDPQQQNIWLETGTQQTDYHSGTHR
                     PYQQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENG
                     ALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSN
                     GNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSALS
                     VNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            4782..6236
                     /gene="mgtE"
                     /locus_tag="ACFM8U_000239"
     CDS             4782..6236
                     /gene="mgtE"
                     /locus_tag="ACFM8U_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC6333548.1"
                     /translation="MSIEQTPPNLENNGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(6322..7230)
                     /gene="hslO"
                     /locus_tag="ACFM8U_000240"
     CDS             complement(6322..7230)
                     /gene="hslO"
                     /locus_tag="ACFM8U_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC6333549.1"
                     /translation="MNQTAIDRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIGEDESLVDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            7475..8230
                     /locus_tag="ACFM8U_000241"
     CDS             7475..8230
                     /locus_tag="ACFM8U_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6333550.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            8249..8467
                     /locus_tag="ACFM8U_000242"
     CDS             8249..8467
                     /locus_tag="ACFM8U_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333551.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            8612..8959
                     /locus_tag="ACFM8U_000243"
     CDS             8612..8959
                     /locus_tag="ACFM8U_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC6333552.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRGIIR"
     gene            8956..9648
                     /locus_tag="ACFM8U_000244"
     CDS             8956..9648
                     /locus_tag="ACFM8U_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC6333553.1"
                     /translation="MNEILRQPSVLLFLTLAVYAIAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            9715..10935
                     /gene="argJ"
                     /locus_tag="ACFM8U_000245"
     CDS             9715..10935
                     /gene="argJ"
                     /locus_tag="ACFM8U_000245"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC6333554.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            10995..12404
                     /locus_tag="ACFM8U_000246"
     CDS             10995..12404
                     /locus_tag="ACFM8U_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6333555.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGATVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            12594..13985
                     /gene="hrpA"
                     /locus_tag="ACFM8U_000247"
     CDS             12594..13985
                     /gene="hrpA"
                     /locus_tag="ACFM8U_000247"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC6333556.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKVEEKYQKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKAIAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYSPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPTGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEENFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            14086..15213
                     /locus_tag="ACFM8U_000248"
     CDS             14086..15213
                     /locus_tag="ACFM8U_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFC6333557.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALRENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            15391..16968
                     /gene="lpt3"
                     /locus_tag="ACFM8U_000249"
     CDS             15391..16968
                     /gene="lpt3"
                     /locus_tag="ACFM8U_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC6333558.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFSRYKTSRLLIALFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSRIPAFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNAIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENEMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEANIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPL
                     VLYSLDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCSEGSVTGNLITGD
                     AGSLNIHDGKAEYVYPQ"
     gene            17070..20495
                     /locus_tag="ACFM8U_000250"
     CDS             17070..20495
                     /locus_tag="ACFM8U_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC6333559.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALLIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEIRQLTSSENQGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYATQQPTPSPEREGRGEGKTVAAQTNFSATAANPLPQERE
                     QSASASTFSDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATSTNPLPQEREQSAS
                     ASTFSDDLRPANLQQPSPSPVGEGWGEGKTVATQTNFSATTANPLPNPLPQEGEQSAA
                     ASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQAWLKTAERENPRLLFLSRDDLMQHA
                     AAHITEEQFPKFWQTADGKFELSYRFEPHHPLDGVTLTLPLTVLNRLHAPSLEWLVPG
                     MLREKIQLLIKALPKQIRRICVPVPDFITQFLSQNPDRNAPILPQLAQAIAKTAGDIR
                     ILEQINQDEWAAFRLPEHCYFNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNT
                     QEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLCDTTEAAEQAH
                     RQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGED
                     ELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRL
                     QTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEK
                     NDGLVKQGLPVSDDLTAFKWMIEELRVSLFAQELKTPYPVSVKRLMKMWEDLN"
     gene            complement(20518..20940)
                     /locus_tag="ACFM8U_000251"
     CDS             complement(20518..20940)
                     /locus_tag="ACFM8U_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC6333560.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEILVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAIGRERLNRLVREIKDAV"
     gene            complement(20944..22590)
                     /locus_tag="ACFM8U_000252"
     CDS             complement(20944..22590)
                     /locus_tag="ACFM8U_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HFC6333561.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIKKSERIYQPEFQKESVEPNTENGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLSIDFIDNDDEDLIATDRTSLDWKHPKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIENWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(22604..23659)
                     /locus_tag="ACFM8U_000253"
     CDS             complement(22604..23659)
                     /locus_tag="ACFM8U_000253"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6333562.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPVKYSGKRLTVRDVLGMENGFPKIMAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            23949..24101
                     /locus_tag="ACFM8U_000254"
     CDS             23949..24101
                     /locus_tag="ACFM8U_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333563.1"
                     /translation="MSGIYLPRLFPPHIAERGLLYFQQGKVLDVRKTSAGHYRAEVCG
                     SENYWV"
     gene            24358..24626
                     /locus_tag="ACFM8U_000255"
                     /pseudo
     CDS             24358..24626
                     /locus_tag="ACFM8U_000255"
                     /inference="COORDINATES: protein motif:HMM:NF017953.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(24716..25165)
                     /locus_tag="ACFM8U_000256"
     CDS             complement(24716..25165)
                     /locus_tag="ACFM8U_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6333564.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINTVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         6504 a         7488 c         5762 g         5552 t
ORIGIN      
        1 atccagcaca aaacgggatc ggtgattctt gtccgcaaga atcgttgatt ttctctatta
       61 cacggataat catcatgcgc ttcacacata ccatcccatt ttattccgta ttgtccaccc
      121 tcggtctttt tgccgtttcc cctgcttact catccattgt ccgcagcgat gtcgattacc
      181 aatattttcg cgactttgcc gaaaataaag gcgcgttcgc cgtaggtgca agcaatattt
      241 ccatccaaga caagcaaggc aaaatattag gcagggttct cagcggcatc cccatgcccg
      301 acttccgcgt cagcaaccgg caaaccgcta tcgccaccct ggttcacccc caatacgtca
      361 acagtgtcaa acacaacgtc ggctacggtt ccatacaatt cggcaacgac acccaaaacc
      421 ctgaagaaca agcctatacc taccgcctcg tatcacgcaa cccgcacccg gactacgact
      481 accaccttcc ccgcctcaac aaactggtta ccgaaatctc acctgccgca ctcagcagcg
      541 tacccttgct tggaaacggc cagccaaagg ccaatgccta cctcgatacc gaccgcttcc
      601 catactttgt acgactcggc tcaggcacgc aacaagtccg caaagcagac ggcacgcgta
      661 cacgaaccgc cccggcatac caatacctga ccggcggcac gccgctgaaa gtattggggt
      721 tccaaaacca cggcttactc gtcggcggca gcctgaccga ccaacccctt aacacctacg
      781 cagtcgccgg agacagcggt tcccccctgt tcgccttcga caagcatgaa aaccgctggg
      841 tgcttgcggg cgtactcggc acctacgccg gcttcgacaa cttcttcaac aaatacatcg
      901 tcacgcaacc cgaattcatc cgttccacca tccgccaata cgaaacccgg ctggatgtcg
      961 ggctgaccac caacgaactc atatggcgcg acaacggtaa tggcggcagc accctgcaag
     1021 ggctcaacga acacatcacc ctgcccattg caaacccttc gcttgccccg caaaacgaca
     1081 gcaggcacat gccgtctgca gatgccggca aaacgctcat cctatccagc aggttcgaca
     1141 acaaaacact gatgctggca gacaatatca accaaggcgc aggcgcattg cagttcgaca
     1201 gcaacttcac cgtcgtcggt aaaaaccaca catggcaagg tgcaggcgtt atcgtagccg
     1261 acggcaaacg cgtcttctgg caagtcagca accccaaagg cgaccggctc tccaaactgg
     1321 gcgcaggcac gcttatcgcc aacggacaag gcatcaacca gggcgacatc agcatcgggg
     1381 aaggcactgt cgtactcgcc caaaaagctg cttcagacgg cagcaaacaa gcattcaacc
     1441 aagtcggcat caccagcggc aggggcacgg ccgtcctcgc cgacagccag caaatcaaac
     1501 ccgaaaacct ctatttcggc ttcaggggcg gacggctcga cctcaacggc aacaaccttg
     1561 cctttaccca tatccgccat gcggacggcg gcgcgcaaat cgtcaatcac aaccctgacc
     1621 aagccgcgac actgacgctg accggcaacc ccatcctcag tcccgagcat gtcgagtggg
     1681 tgcaatgggg caaccgtccg caaggcaacg cggcggttta cgaatacatc aacccgcacc
     1741 gcaaccgtcg gaccgactac ttcatactca aacccggcgg caacccgcgc gaatttttcc
     1801 cgttaaatat ggaaaactca acaagctggc aatttatcgg caacaacagg caacaggccg
     1861 ccgaacaagt cgcccaagcc gaaaatgccc gccccgacct aattaccttc ggcggatact
     1921 tgggtgagaa cgcgcaaacg ggcaaagccg caccgagtta cagcaaaacc aatatcgaat
     1981 gggtgaatgg tccaaacacc ccacacaagc cctcgcccta tacgaatacg ccaacccgca
     2041 caggggcagg cgtaccgacg attaccgcct caaagaaagc ggcaaccccg gtgcctactt
     2101 cccatggaat gcctacagca cccaagactg ggaatttctc ggacatgacc ggcgaagcag
     2161 ccatagaaaa aacccgccat atcgcaaatg ccgccgtata cggccggccc gaataccgtt
     2221 acaacggcgc actcaacctg cactatcgtc ccaaacgcac cgacagcacg ctgttgctca
     2281 acggcggcat gaaccttaac ggggaagtct tgattgaggg cggcaatatg attgtgtcag
     2341 gcaggcccgt accccatgcc tacgaccacc aggccaaacg cgaacccgtt cttgaaaacg
     2401 aatggaccga cggcagcttc aaggctgcac ggttcaccct gcgaaaccat gcccgactga
     2461 cggcagggcg caataccgcg catctggacg gcgacataac cgcatacgat ctgtccggca
     2521 tcgacctcgg ctttatccaa ggcaaaacac cggaatgcta ccgctcctac catagcggca
     2581 gcacccactg cacacccaac gccgttttaa aagccgaaaa ctatcgtgca ctacctgcaa
     2641 cgcaagtacg cggcgacatt acccttaacg accgttcaga gctccgcctg ggcaaagcac
     2701 acctgtacgg cagcatccgt gccggcaaag acaccgcagt ccgcatggaa gcagacagca
     2761 actggacact ttcccagtcc agccacaccg gcgcactgac gcttgacggc gcacaaatta
     2821 ccctgaaccc cgatttcgcc aataatacac acaacaaccg cttcaacaca ctgaccgtca
     2881 acggcacact tgacgggttc ggcacattcc gattcctgac cggcatcgtc cgaaaacaaa
     2941 atgccccccc cctcaaactg gaaggggaca gccgcggcgc attccaaatc cacgtcaaaa
     3001 acaccggaca agaacctcaa acaaccgaat cgcttgcact tgtgagcctc aatccgaaac
     3061 acagccacca agcccgattt accctccaaa acggctatgc cgatttgggt gcctaccgct
     3121 acatcctccg caaaaacaac aacggataca gcctgtacaa cccgctcaaa gaggccgaac
     3181 ttcaaattga agccacgcgt gcggaacatg agcgcaacca acaggcatac aaccaattac
     3241 aggcaaccga catcagcaga caggttcaac atgactctga cgcgaccagg caggcactac
     3301 aggcctggca gaacagtcaa accgaacttg cccgcatcga cagccaagtc caatatctgt
     3361 ccgcccaatt gaaacagaca gacccgctga ccggcattct gacgcgtgcc caaaacctgt
     3421 gtgccgcaca aggatacagt gccgatatct gccgtcaggt tgccaaagcc gccgacacga
     3481 acgacctgac actcttcgaa accgaactgg atacgtatat agaacgtgta gaaatggccg
     3541 aatccgaact tgacaaagca cggcaaggcg gcgatgcgca agccgtcgaa acagcccggc
     3601 acgcctacct gaacgcactc aaccgtctgt cccgacaaat ccacagtttg aaaaccggcg
     3661 ttgccggcat ccgtatgccg aacctggccg aactgatcag ccggtcggcc aacaccgccg
     3721 tttccgaaca ggccgcctac aataccggcc ggcaacaagc ggaacgccgc atcgaccgcc
     3781 accttaccga tccgcagcag caaaacatct ggctggaaac cggtacgcaa caaaccgact
     3841 accatagcgg cacacaccgt ccctaccaac aaactaccaa ctatgcacat atcggcatcc
     3901 aaaccggcat caccgaccgt ctcagtgtcg gtacgatttt aaccgatgag cgcacaaaca
     3961 accgttttga tgaaggcgta tccgcccgaa accgcagcaa cggcgcacat ctgttcgtca
     4021 aaggggaaaa cggcgcactc tttgccgcgg cagatttagg ctacagcaac agccgtaccc
     4081 gatttaccga ttatgacggg gctgccgtcc gccgccacgc atgggatgca ggcatcaaca
     4141 ccggcatcaa aatcgatacc ggcatcaacc tcagacccta tgccggcatc cgtataaacc
     4201 gcagcaacgg caaccggtac gtactcgacg gcgcagagat aaacagcccg gcgcaaatcc
     4261 aaaccacatg gcatgccggc atccgtctcg ataaaaccgt cgaactgggt caagccaagc
     4321 tgacccccgc cttcagcagc gattactacc atacccgcca aaacagcggt tccgccctca
     4381 gcgtcaacga ccgtacctta ctgcagcaag ccgcccacgg cacactgcat accctgcaaa
     4441 tcgacgccgg atacaaaggc tggaacgcca aacttcatgc cgcttacggc aaagacagca
     4501 acaccgcccg ccacaaacag gcaggaatca aaataggcta caactggtaa caagccgata
     4561 aaaatgccgt ctggaacccg cgtttcagac ggcatttgcg ttaaaaatag taaaccgttc
     4621 caaaagggag tagaatagtg ccgtttccaa ccctgcgcct gtaccgtcag gcttttatta
     4681 tggaccttcc cagttcgttt ttactgaaca ccccatccga ttcaaacccg caataaccat
     4741 cccgccggaa tgtctccccg cacacggcgg gcggagcatt tatgagcatc gaacaaaccc
     4801 ctccgaacct tgaaaacaac ggtatcgaaa acgatgtaga acgcgtttcc gccgatttcg
     4861 accgtgtcca ctccctctgc gaaatcctcg aacctgcttt tgaacaaatc gaaaacggca
     4921 caccgctcga agacgcgccg ctgcgcgaca agctgaccga gctgaccgtc ctcttgagcg
     4981 agctgcaccc tgccgacgtg gcggcggtat tggaatcgct gccgccgcgc gaacgcaata
     5041 tcgtctggat tttggtcaaa ccggaagacg acggcgaagt attgctggaa gtatccgacg
     5101 cggtgcgcga aacgctgatc gagtcgatgg acaaggacga attgttggcg gcagttgacg
     5161 atttggacgc ggacgaattg gcggaactgg cagacgattt gccgcaccaa gtggtttacg
     5221 aagccttaca gacgcgtgat gaggaagaac gcgcccaagt caaggcggca atgtcctacg
     5281 aagacaacca agtcggtgcg attatggact tcgagttggt cagcatccgc gctgatgtcg
     5341 cctgtgaagt ggtgctgcgc tatctgcgcc gcttcgacag cctgcccgac cataccgaca
     5401 agatttttgt ggtcgatgaa aacgacgtgt tgcagggcgt gctgcccatc cgcaaacttt
     5461 tggtcgccga tcccgaagat ttggtggaaa acgtgatggc gaaagatgtc gtgcgtttcc
     5521 gcgccgaaga cgacgtggaa gaagcggcgc aggcgtttga acgctacgac ttggttaccg
     5581 cgcccgtcgt cgatgaaaac aaaaagctca tcggcaggat taccgttgac gagatggtgg
     5641 acgtaatccg cgaagaatcg gaagcggata tgctgaatat ggcgggtttg caggaagagg
     5701 aagacctgtt cgcccccgtc ttggattccg tcaaaaaccg ctggatgtgg ctcgccgtca
     5761 acctctgcac cgccttcctc gccagccgcg tcatcggcgc gtttgaaggc agcatcgaaa
     5821 aaatcgtcgc actcgccgcg ctgatgccca tcgtcgccgg tatcggcggc aactcgggca
     5881 accagacgat taccatgatt gtccgcgcaa tggcgatggg gcagttgacg ggaatgcagg
     5941 cggggcgctt gttgaaaaaa gaagtcggcg ttgccctagt caacggcatt atctggggaa
     6001 ccgtcatggg cgcggtttca tggctgctct acggcagcct cggcatcggg ctggttatga
     6061 ttgccgcgat gacgctcaac ctcctgctgg cggcaaccgt cggcgtattg attcccgtgg
     6121 taatggaaaa gttcggacgc gaccccgcgc tgggcagctc ggtgctgatt acggcggtaa
     6181 cggattccgg cggcttcctg attttcttgg gtctcgccac cctgttcctg ctttaaatgc
     6241 cgtccggacc cgcgcaaaaa tgccgtctga agcggaagct gcttcagacg gcatttgact
     6301 atttatcctt gttgcacaag attattggac ggtatgccgg ggcagccctt tggcaacgcc
     6361 gaccacatcc tccccgaaca gcgcgttgac atcggtttcg tcaaacacat atttgctgtg
     6421 gcagaaatcg caatcgactt cgatgctgcc ttgttccgcc accacgccgc cgacttcttc
     6481 cccgcccagc atcaacagca tatcgctgac tttgccgcgc gaacaggtgc atgaaaattc
     6541 aaacgtttcc ggctcgaaca cgcgcggcgg cgtttcgtgg aacaagcggt acaaaacatg
     6601 ttgcgcatcc aattctgcca actcgcccgc cgtcagcgtg cgcgccagcg tactgacgtg
     6661 ttcccatgcc tcttcatcca acacctcttc aggcagacgc tgcaccagca gaccgcccgc
     6721 cgcttcatcg cttgcagaca ggacgatgtg cgtatcaagc tgttcggaac gtttcatata
     6781 gttcatcaac atttgcgcga taccgtcgcc ttccaaaggc actacgccct gccagggttc
     6841 gccgtctttg ggctgcagcg tcagcacgaa tacgccgccc tcgcccaaaa gatcgaccaa
     6901 actttcatct tcaccgattt ctgccgcctc gtcccaccgc gcggttgcac ggacggtacg
     6961 gtcggaagtc gcttctgcaa ccagcatttt cagccgcccc tgcccctgaa cctgcacaat
     7021 cagcgtgcct tcgtttttaa ggttgcccga cagcagaacg ccggcagcca gtagctcacc
     7081 caaggcgcgg cggatggcgg cgggatagtt tttctgtttt acaatgtgct gccacacgtt
     7141 ttccagacgg acgtgcagcc cgcgcacggg catatcgtcg aagataaagc gggtacgcac
     7201 atcggcgcgg tcgatggcgg tttgattcat gattttctct gactgattgt tcggatggcg
     7261 gctatatggt tgcggtcggc gcgaaaacaa gacggacggc ggatgcgctt cccaaattat
     7321 caataaatta tataaaaatc aacatattaa ctcaatctaa caagccgttt tttgccaaac
     7381 agccgttttt ttatatacaa tcaacaagat attttcgact gatacagcat aacatcgcac
     7441 ggcggcacga tgcctcctgc gcggaaacac cgatatggat tcttttttca aaccggcagt
     7501 ttgggcggtt ttgtggctga tgtttgccgt ccgccccgcc cttgccgacg agttgaccaa
     7561 cctgctcagc agccgcgagc agattctcag acagtttgcc gaagacgaac agcccgtttt
     7621 acccatcaac cgagcccccg cccggcgggc gggcaatgcc gacgaactca tcggcagcgc
     7681 gatggggctt aacgaacagc ccgttttacc cgtcaaccga gtccccgccc ggcgggcggg
     7741 caatgccgac gaactcatcg gcaacgcgat ggggcttaac gaacagcccg ttttacccgt
     7801 caaccgagcc cccgctcggc gggcgggcaa tgccgacgaa ctcatcggca acgcgatggg
     7861 acttttgggt attgcctacc gctacggcgg cacatcggtt tctaccggtt ttgactgcag
     7921 cggcttcatg cagcacatct ttaaacgcgc catgggcatc aacctgccgc gcacgtcggc
     7981 agaacaggcg cggatgggta cgccggttgc ccgaagcgaa ttgcagcccg gggatatggt
     8041 gtttttccgc acgctcggcg gcagccgcat ttcccatgtc ggactttata tcggcaacaa
     8101 ccgcttcatc cacgcgccgc gcacggggaa aaatatcgaa atcaccagcc tgagccacaa
     8161 atattggagc ggcaaatacg cgttcgcccg ccgggtcaag aaaaacgacc cgtcccgctt
     8221 tctgaactga tttcccaagg aatacgcaat gagtatgccc gaaatgccca aatggtacga
     8281 cgatgacgga cagatcgtgt cctgtaccga aaaggtcaaa gtgatgtccg aaaatatggc
     8341 cgagctgtat cagacggcac aagacgcgtt tgaagacgcg ctgctgatgg gttgcggcga
     8401 acgtcagttg cgcgattacc tgctcgcgct gattgaaggt ttggaaaatc cctaccgcaa
     8461 agtctgaaca cgccccggtt gctgcggcac ggtttatccg tgccgttttt gcgtttgtgc
     8521 gcggcttcgg cttttcagac ggcatatttg acgttatgat taaacagtta acaagattta
     8581 tcacaacgcc gtcaaaagac agacacacaa catgaacatc atccgcgcgc tcctcatcat
     8641 cctcggctgc ctcgccgccg gcgaaaccgc cgttttccta gcaggcatca aactgcccgg
     8701 cagcatcgtc ggcatgggcg tgctgtttgc gcttttgcag gcgggttggg tcaaaacgtc
     8761 ttggctgcaa cagcttaccg acgcgctgat ggcgaatctg acgctgtttc tcgtgccgcc
     8821 ctgcgtggcg gttatcagct atttggattt gattgccgac gattggtttt cgatactggt
     8881 ttccgcctcc gccagcactt tgtgcgtact gctggttaca ggcaaggttc accggtggat
     8941 acggggtatt atccgatgaa cgaaatcctc aggcagccca gcgttctgct tttcctcacg
     9001 cttgccgtgt acgcgattgc gattatcgtg cgcacgcgca cgggtaatat cttctgcaac
     9061 cccgtactcg tcagcactat cgtgctgatt gcctacctga aaatcctcgg tatcgattat
     9121 gcggtgtacc acaacgccgc gcaattcatt gatttttggc taaaacccgc cgtcgtcgtg
     9181 cttgccgtgc cgctctacca aaaccgccgt aaaatcttca accagtggct gcccgtcatc
     9241 gtttcacagc ttgcgggcag cgttacgggc attgttacgg ggatgtattt tgccaaatgg
     9301 ctgggcgcgg aacgcgaagt cgtcctctcg ctcgcgtcca aatctgttac caaccccatc
     9361 gccattgaaa tcacccgctc catcggcggc attcccgcca ttaccgccgc caccgtcatc
     9421 attgccggtc tggtcggaca gattgccggt tacaaaatgc tgaagaacac agtcgtcatg
     9481 ccctcgtccg tgggtatgtc gctcggcacg gcttcgcacg cgatggggat tgccgcctcg
     9541 ctcgaacgca gccgccgcat ggcggcatac gcgggactgg ggctgacgtt caacggcgta
     9601 ctgaccgcgc tgattgcgcc gctgctcatc cccgttttgg gattttgaac ccgtttcaga
     9661 cggcatttca gcccatgctg tctgaacgcc gacacactcg caaggagaac cgttatggct
     9721 gtcaacctga ctgaaaaaac cgccgaacaa ctgcccgaca tcgacggcat tgccctctac
     9781 accgcccaag caggcgtgaa gaagcccggg cataccgacc tgacactgat tgccgtagcc
     9841 gccggcagca ccgtcggtgc agtcttcacg accaaccgtt tctgtgccgc gcccgtccac
     9901 atcgccaaat cgcacctttt cgacgaagac ggcgtgcgcg ccctcatcat caacacgggc
     9961 aacgccaacg cgggtacggg cgcacagggc agaatcgatg ctttggcagt gtgtgccgcc
    10021 gccgcccggc aaatcggctg caaaccgaac caagtgctgc ccttctccac cggcgtgatt
    10081 ctcgaaccgc tgcccgcaga caaaatcatc gccgcgctgc ccaaaatgca gcctgccttc
    10141 tggaacgaag cggcacgcgc catcatgacc accgacaccg tgcccaaagc cgcctcgcgc
    10201 gaaggcaagg tcggcgacaa acacaccgtc cgcgccacgg gcatcgccaa aggctcgggc
    10261 atgattcatc ccaatatggc gaccatgctc ggtttcatcg ccaccgatgc caaagtttcc
    10321 caacccgtcc tccaactgat gacgcaggaa atcgccgacg aaaccttcaa caccatcacc
    10381 gttgacggcg acaccagcac caacgacagc ttcgtcatca tcgccaccgg caaaaacagc
    10441 caaagcgaaa tcgacaacat cgccgacccg cgttacgccc aactcaaaga attgttgtgc
    10501 agcctcgcgc tcgaactcgc ccaagccatc gtccgcgacg gcgaaggtgc gaccaagttc
    10561 atcaccgtcc gcgtcgaaaa cgccaaaacc cgcgacgaag cccgccaagc cgcctacgcc
    10621 gtggcacgtt cgccgctggt caaaaccgcc tttttcgcct ccgaccccaa cctcggcagg
    10681 ctgctcgccg ccatcggtta tgccggcgtt gccgacctcg ataccgacct cgtggaaatg
    10741 tatctcgacg atattttggt tgccgaacac ggtggacgcg ccgcaagcta caccgaagca
    10801 caagggcagg cggtgatgtc gaaggccgaa atcaccgtcc gcatcaagct gcatcgcgga
    10861 caagccgccg ccaccgtcta tacctgcgac ctgtcgcacg gatacgtttc catcaacgcc
    10921 gactaccgtt cctgactcga cacggcttca gacggcatac ataaaatgcc gtctgaaccg
    10981 ccggacaaca taccatgacc tccacattcc cccgccgcct cgcccgcaaa atccgccaaa
    11041 cccgccgcct gtcgcgcaaa agcatcgcct ttctgttcct tttggcaggt tcggcactcg
    11101 tcgccctgac cgcgctgttt tttgcccatc ttgccgattt tgcgctggaa ctgaacgcca
    11161 aactggttca acaatacccg tggttcgcgt gggtcgcgct tcctttgggt ttaccgctta
    11221 ttgcgtggct cacacgcaaa ttcgccccct tcaccgccgg cagcggcatc ccgcaggtca
    11281 tcgcctcact gtcgctgccc tacggcgcac agaaaacgcg gctgatccgc ctcgggcaga
    11341 cgctgctgaa gattccgcta acctttttgg gtatgctgtt cggcgcgtcc atcggacgcg
    11401 aaggtccgtc cgtgcaggtc ggcgcgacag tgatgggcgc gtggggcgcg tggtgcaaga
    11461 aacacggctt ggcattcaaa gggatgcagg aaaacgattt gatggcggcg ggcgcggcgg
    11521 gcggtttggc agccgcgttc aacgcgccgc tggcgggcgt gattttcgcc attgaggaac
    11581 tcgggcgcgg catcatgttg cgctgggaga ggcaaattct tttgggcgtg ctcgcctccg
    11641 gtttcataca ggtcgccatt cagggcaaca acccgtattt ttccggcttc aacggcggcg
    11701 tattggaaca tatctttctg tgggtcgcac tgtccggcct ggtttgcggc gcggcgggcg
    11761 gcctgttcgg acgtttgctc tatcgcggtg cggcggcgtt tgcaccgcgc aagatacgcg
    11821 gcttcatccg caaccgtccg ctgctgctgg cggcactgat ggggctgctg ctcgccctgc
    11881 tcggcacgtt ctaccaaggc aaaacctacg gcactggcta ccacgaagcc gcccaagccc
    11941 tgcacggcat ctacgaagcc cccttcggac tcgccgccgc caaatggctc gccaccgtat
    12001 tcagctattg ggcaggcatt ccgggcggca ttttcactcc ctcgctgacc ataggcgcgg
    12061 ttttgggcga gcatatcgcc gccatcgccg acatatcgca gggtgcaaac atcatcgtcc
    12121 tcatctgcat ggcggcattt ctggcgggcg cgacacaatc cccgattact tccgccgtcg
    12181 tcgtcatgga aatgacgggc ggacaaagcc tgctgttttg gatgctaatt gcctgcattt
    12241 tcgcctcgca ggtttcgcgc cagttttcgc cgcgtccgtt ctaccacgca tcgggaatgc
    12301 gcttccgcca gcgcgtgctt caagaaaccg ccgcccaaac cggcaatgcg cccgcaagac
    12361 cgcaaacagc aaacagcaaa acgggaatgc cgtctgaaaa ttaaaacgcc cccgatcaaa
    12421 cgccggcagc cgccttgatt tgaataccgt tccgccgccg cttgaaattt cagcaacaat
    12481 gccgtctgaa cgacagaatg cggttttcag acggcatttc cccatcccga tattgcctaa
    12541 acaaaaccga agcgtttgct ataattctat tttttaccgc acacgcacca atcatgtttc
    12601 ccgatttctc ccaaaccctc tccaaagacc gccacttcct acgttccgcc ttcaaaaatc
    12661 ccaacaaata cggcggcttg tccaaagtcg aagaaaaata ccaaaaatcg cacgaaatct
    12721 ttttgaagcg tttggctgcc ttgcccaaac ccgaattcga taacaccctg cccgttcacg
    12781 agaagctcga cgaaatcaaa aaagccattg ccgagaatca ggtaacgatt atttgcggcg
    12841 aaaccggttc gggcaagacc actcagttgc ccaagatttg tctggaactc gggcgcgggg
    12901 cggcgggttt gatcgggcat acccagccgc gccgtttggc ggcgcgttcc gtagcagagc
    12961 ggattgccga agagctgaaa tccgaaatcg gcagcgcggt cggctataaa gtgcgcttca
    13021 ccgaccacac ctcgcgcgat gcctgcgtca agctgatgac cgacggcatc ctgctggcgg
    13081 aaacccagac cgaccgttat ctcgccgcct acgacacgat tatcatcgac gaagcgcacg
    13141 agcgcagcct gaacatcgac ttccttttgg gctatttgaa acaactcctg ccgcgccgcc
    13201 ccgatttgaa agtcatcatc acctcggcaa cgatagacgc agaacgcttt tcccaacact
    13261 tcaacggcgc gcccgtttta gaagtgagcg ggcgcaccta tcccgtcgaa atcctctaca
    13321 gcccgctgac cggcaaagac gaagacgacg cagaagtcga gctgaccgac gcgattgtcg
    13381 acgcggcgga cgaattggca cgctacggcg aaggcgatat tttggtgttc ctgccgggcg
    13441 aacgcgaaat ccgcgaagcc gccgaagccc tgcgcaaatc cacgctacgc cgcaacgacg
    13501 aaatcctgcc cctgttcgca cgcctgtcgc acgccgagca gcacaaaatc tttcacccca
    13561 caggcgcgaa acgccgcatt gtgttagcaa ccaacgtcgc cgaaacctcg ctcaccgtgc
    13621 cgggcatcaa atacgtcatc gacaccggcc tcgcacgcgt caaacgctat tccgcacggg
    13681 cgaaagtgga gcagctccat gtcgaaaaaa tctcccaagc cgccgcccgc caacgctccg
    13741 gccgctgcgg ccgcgtctcc gcaggcgtat gtatccgatt gttttcagaa gaaaatttca
    13801 acaaccgccc cgaatttacc gaccccgaaa tcgcccgcag caacctcgcc gccgtcatcc
    13861 tgcgcatggc atcgctgaaa ctcggcgacg tagcggcatt cccattttta gaaatgcccg
    13921 attcgaggta tattaatgat gggtttcagg tgttgctgga attaggggca gtggaggccg
    13981 cctgaaaaca agtaggatat acacttctac ctgacaaaga ccaacaaacg ataaaactac
    14041 ggcacatcta gatgcccaac ctacaacatt gcacacggta aaaacatgaa acacttactc
    14101 atcgattttg aaaacgtcca gccgcaaaac ttagacaaat tactaaccga aaatacccat
    14161 atttggctat ttataggtgt attacacaaa atgttaccta ttagtctggt gcaatcccta
    14221 ctacgtttcg gcgaacgtgt ccatcttgtc cagttacaaa aaacagggaa aaacgcattg
    14281 gatttttacc tgtcctatta cctcggacaa attaccgcca cagaccccaa tgcccaaatc
    14341 ggcatactct cgcgtgatgg aggatacgat gttctggtcg aacatatttt gaaaaaccac
    14401 caggcgaagg gtatcgtgcg cctagccaat atagatgaag tacaacatca gaaaattact
    14461 accgaaccgc cgtcagcatt gcgggaaaac actcctcagc ctgaaaccac cctcaaacca
    14521 cagcaaccat taacttccta tttccaagca gccctaactg cactgcgccg ccccgacgct
    14581 ttccgcccct gccgcctgca taacctgcga caaaatctgc gtaagcatat tttgagtgat
    14641 ttgtttaaag aaaaaaccga tgaagaatgc gaaataacca ctgctaacgt tatcaataaa
    14701 ctcaaagcac aaaacttcat cagcattgat gaacaggaaa ccgtttccta ccatctcagt
    14761 gataatgatt tgttacaaag aatccaacgc catattttaa gccaacgtcc caaaacctac
    14821 gctgattttc aagccgtcgt gcaaaaccga gcagatgcac ttcacttaac agtcggtacc
    14881 aacgacattc aatcctttgc gcgacatttg cgcgaccaaa acctgatccg ccaaaacaat
    14941 gggaaaattg aatatgcacc gtttactgaa cctaaaccac agccaacgcc caagcagcct
    15001 aaaaaaaccg catgggaacc tgatgaaatt atttggaaaa aagtgattgc cgcgttatcg
    15061 ttaaagaacc gtcctaataa aaccaaaact ttacgcaata caatccaggc actcacaaaa
    15121 tccaatgcac aagaaactga caaactgcta caacatttac aagataccca agtcctacgt
    15181 attgacggta ctaaaattgt gtatatcaag taggatgggc atttatgcct gacctacaaa
    15241 accaaaaaca acctaccacc cttaatcaac tccacaaacc cttttcagac aacctcgttt
    15301 tttgaaaaac aatctgtaaa cagatagctg ctgaagaata ccgttgccga gccccaaaac
    15361 ccgtcctgca acttttattg tgaactgccc attatgaaaa aatccctttt cgttctcttt
    15421 ctgtactcat ccctacttac cgccagcgaa atcgcctatc gctttgtatt cggaattgaa
    15481 accttaccgg ctgcaaaaat ggcagaaacg tttgcgctga catttgtgat tgctgcgctg
    15541 tatctgtttt cgcgttataa aacttcgcgg ctgctgattg cgctgttttt cgcgttcagc
    15601 attattgcca acaatgtaca ttatgcggtt tatcaaagtt ggatgacggg tattaactat
    15661 tggctgatgc tgaaagaggt taccgaagtc ggcagtgcgg gcgcgtcgat gttggataag
    15721 ttgtggctgc ctgcgttgtg gggcgtgttg gaagtcatgt tgttttgcag ccttgccaag
    15781 ttccgccgta agacgcattt ttctgccgat atactgtttg ccttcctaat gctgatgatt
    15841 ttcgtgcgtt cgttcgacac gaaacaagag cacggtattt cgcccaaacc gacatacagc
    15901 cgcatcaaag ccaattattt cagcttcggt tattttgtcg gacgcgtgtt gccgtatcag
    15961 ttgtttgatt taagcaggat tcccgccttt aagcagcctg ctccaagcaa aatcgggcag
    16021 ggcagtgttc aaaatatcgt cctgattatg ggcgaaagcg aaagcgcggc gcatttgaag
    16081 ctgtttggct acggacgcga aacttcgccg tttttaaccc ggctgtcgca agccgatttt
    16141 aagccgattg tgaaacaaag ttattccgca ggctttatga cggcagtgtc cctgcccagt
    16201 tttttcaatg cgataccgca cgccaacggc ttggaacaaa tcagcggcgg cgataccaat
    16261 atgttccgcc tcgccaaaga gcagggctat gagacgtatt tttacagcgc gcaggcggaa
    16321 aacgagatgg cgattttgaa cttaatcggt aagaaatgga tagaccatct gattcagccg
    16381 acgcaacttg gctacggcaa cggcgacaat atgcccgatg agaagctgct gccgttgttc
    16441 gacaaaatca atttgcagca gggcaggcat tttatcgtgt tgcaccaacg cggttcgcac
    16501 gccccatacg gcgcattgtt gcagcctcaa gataaagtat tcggcgaagc caatattgtg
    16561 gataagtacg acaacaccat ccataaaacc gaccaaatga ttcaaaccgt attcgagcag
    16621 ctgcaaaagc agcctgacgg caactggctg tttgcctata cctctgatca tggccagtat
    16681 gtgcgccaag atatctacaa tcaaggcacg gtgcagcccg acagctatat tgtgcctctg
    16741 gtgttgtaca gcctggataa ggccgtgcaa caggctgcca accaggcttt tgcgccttgc
    16801 gagattgcct tccatcagca gctttcaacg ttcctaattc acacgttggg ctacgatatg
    16861 ccggtttcag gttgtagcga aggctcagta acgggcaatc tgattacggg cgatgcaggc
    16921 agcttgaaca ttcacgacgg caaggcggaa tatgtttatc cgcaataagc ggcgtgaaaa
    16981 ccaataaaga caaatttaga tgatgtcggg gaagatgccc gaccgacaag actatgcaaa
    17041 atatgaaaaa ccaaatacgc ggatcaggca tggatgcacg atccaacccg gccaatgttt
    17101 cagacggcct gcaaaacagt tcgggccata tcggtaccaa cacgcgctac cgtctgacca
    17161 aactcggcga acaaatggcg cgcctgccca tcgacccgaa aatcgcgcgc attttgttgg
    17221 cggcgaagaa gcacgactgc atggcggaaa tattggtgat tgcgtccgcg ctattgattc
    17281 aagacccgcg cgagcggccg ctggaggcac gcgatgccgc cgccaaggcg catgaacgct
    17341 ttaccgacaa gcaatccgat ttccttgcct acctgaatat ttgggacagt ttccagcgcg
    17401 agcgcgacaa aggtttgtcc aacaagcagc tggtgcaatg gtgccgccaa tatttcctgt
    17461 cgcacctgcg tatgcgcgaa tggcgcgagc tgcaccacca gcttgcccaa accgcgattg
    17521 aaatgggttt gaccaccaag gaagccgctt tcagacgacc tcccgaaatc aggcagctca
    17581 cgtcgtctga aaaccaaggc gaccaagacc tatccgccaa actcaagcaa aaacaactgg
    17641 ataaaaagca acaccgcgcc caaatccgcg ccgccaaaga agcgggctac gaacaaatcc
    17701 accgcgccct gctcactggc cttatcgcca acgtcggcat gaaatcgccc gacggcaacg
    17761 actacaccgg cgcgcgcggc agccgcttcc accttttccc cgcctccgcc ctgttcaaag
    17821 ccaagcccaa atgggtgatg gcggcagaat tggttgaaac cacgcgcctt tacgcgcgcg
    17881 acgtcgccgt tatccagccc gaatggatag agcaggaagc gccgcacctc gtccgctatc
    17941 attatttcga gccgcattgg gaacaaaaac gcggcgaagt cgtcgccagc gaacgcgtga
    18001 cgctttacgg cctgaccgta ttgccgcgcc gccccgtgtc ttacggcaaa gttgcccccg
    18061 aagaagcgcg cgaaatcttt atccgcagcg cgttggtggc gcaggaatgc gatttgaaag
    18121 cggatttttt tgtccacaac aaaaagctga ttaaagaaat taccgaactc gaacacaaat
    18181 cgcgcaagca agacgtgctg gtcgatgacg aagccctgtt tgcgttttat aacgaacgac
    18241 tgcccgaaat ggcttggaaa gacgcgcaag gcagcgtttg gggaagtgaa gattccgtac
    18301 ggattattga atctgacaaa gccgagaggt cgtctgaaaa tgagcgcaac gagtttcgta
    18361 aaaacaagcg taatgggtct cgccaaaatg aaaatcacgg caacaccgta ggttgggttg
    18421 aaaacccaac atcagccgca actgcaaaaa ctgttgggtt tgacaaccca acctacgcta
    18481 ctcagcaacc caccccctcc cccgagaggg agggtcgggg agagggcaaa acagttgccg
    18541 cacaaaccaa cttttccgca accgcagcaa accctctccc gcaggagagg gaacagagtg
    18601 cctcagcttc aacgttttca gacgacctgc gtcctgcaaa tctgcagcaa accgccccct
    18661 cccccgtggg ggagggctgg ggagagggca aaacagttgc cacacaaacc aacttttccg
    18721 caacctcaac aaaccctctc ccgcaggaga gggaacagag tgcctcagct tcaacgtttt
    18781 cagacgacct gcgtcctgca aatctgcagc aaccctctcc ctcccccgtg ggggagggct
    18841 ggggagaggg caaaacagtt gccacccaaa ccaacttctc cgcaaccaca gcaaaccctc
    18901 tccctaaccc tctcccacag gagggggaac agagtgccgc agcttcagcg gtttcaaacg
    18961 accctcaacc ccaaaagcag ccagcacccc aaaaagaccg tctgaaaccc ctccccctcg
    19021 ccgacatccg caccttccaa gcctggctca aaaccgccga acgcgagaac ccgcgcctgc
    19081 tgttcctcag ccgcgacgat ttgatgcaac acgccgccgc acacattacc gaagaacaat
    19141 tccccaaatt ctggcaaacc gcagacggca agttcgaact cagctaccgc ttcgagccgc
    19201 accacccgct cgacggcgtt accctcaccc taccgctgac cgtcctcaac cgcctgcacg
    19261 cgccgtcgct cgaatggctg gtgcccggta tgttgcgcga aaaaatccag ctgctgatta
    19321 aagctctgcc gaagcaaatc cgccgcattt gcgtgcccgt acccgatttc atcacccaat
    19381 ttttaagcca aaaccctgat cgcaacgccc ccatcctgcc ccaacttgcc caagccatcg
    19441 ccaaaaccgc aggcgacatc cgcatactcg agcaaatcaa ccaagacgaa tgggccgcgt
    19501 tcaggctgcc cgaacactgc tatttcaacc tccgcattat cgacgacggc ggacaagagc
    19561 ttgccggcgg ccgcaaactg catgaattgc aacaacagct cggccaagcc gccgccgtta
    19621 ccttccgcga caacacccaa gaattcgagc gcgacaacgt gaccacatgg gacattggca
    19681 ccctgcccga atccatcaaa ttcgcacgcg gcaaacaaca gctcaccggc tacctcggtc
    19741 tgcaaaaaga aaaagacggc cgcatcgccc tgcgcctgtg tgacaccacc gaagctgccg
    19801 aacaggcaca ccgtcaaggc gtcatcgaat tgatgaagct gcaattaaaa gagcaggtaa
    19861 aagacctgaa caaaggcatc caaggcttta cccaagccgc catgctgctc aaacacatca
    19921 acgccgacac cctgcgcgac gacctcaccc aagccgtttg tgaccgcgcc tttatcggcg
    19981 aagacgagct gccgcgcaac gaaaaagcct tcaaagaaca aatcaaacgc gcccgcagcc
    20041 gcctgcccgc cgtcaaagaa gccctcagcc gctacctgca ggaaaccgcc gccgcctacg
    20101 ccgaactgaa cggcaaactc ggcaaacacc cattgaccca ccttctaaga ctacgcctgc
    20161 aaaccctgct cgccgccggc ttcgccaccc gcaccccgtg ggcacaatgg ccgcgcctcc
    20221 ccatctacct caaagccatg accctgcgcc tcgaaaaata cagcagcaac cccgcccgcg
    20281 acgcagcccg cgaagccgat atacaggaat tggaacaaat gtggcaggaa aaaaacgacg
    20341 gtttggtgaa acaaggacta cccgtttcag acgacctcac cgcgtttaaa tggatgatcg
    20401 aagaattgag ggtgtcgctg ttcgcgcagg aattgaagac gccttatccg gtgtcggtga
    20461 agcggttgat gaagatgtgg gaggatctta attaaaggaa gatgccgtct gattttttca
    20521 gacggcatct tttatttccc tgaccaaccg atttaatcgc tccctaccaa tagctttatt
    20581 ttttaattcg cactcccaaa cgacaatcac tttgaaaccg atcttttcca gctcggtttc
    20641 atttttaata tcacgttcgc aattttttgt gattttttcc aaccaaaaat ccgtattgct
    20701 tttcgggata tgtcctttat tacaagaatg cccgtaccaa aaacagccgt gtataaacac
    20761 gactgtttta tatttcggca aaacgatatc cggtttgcct gcatagcgtt tatcgttttt
    20821 ccgataacga aaaccttggg agaaaagaaa tttccttacc aatatttccg gtttggtttc
    20881 tttgctatgg atttttgaca tcacgaaaga acgtttggat gtagtgaaaa tatcggtcat
    20941 aaattacttg gaagatacca ccttcgatcg tttttttgtc tgttccactt ttcttaacaa
    21001 atacgaaacc aagcttatta aatccaaagc atccttatca ttaaaaatac taggagataa
    21061 atttgctctt aactcatgtt gtacaggatt tctaaatcca gtccatgttc cgcatgcaag
    21121 ctgttcaaga ccatcttcta aattttgttc cgccttcgtt ttgttattcg ttaatagaag
    21181 tatagaattt ttactcccga aagcttgttc aatcaattct cttccgtcta tgctatgaag
    21241 acctgtttgc ttctgaacct tatcttcgaa aactttaact gcctcgattg cagcacttaa
    21301 ataatccttc ttttcattgt aaaaatcatt acaggcagta tgcaaatcct gatgcaaatg
    21361 ccgccaatga tagtaaggat attctggaat aatactatgt aaggcagaga ttacttccgc
    21421 ttgctcttta tttgtaagct ccgaatcttc caaacgattc aaaataactt ctactggttc
    21481 cctttccttt tctggcaatt tgtttttcca attttcgata tcaaccccgc tttcaccttt
    21541 cacttcgtca tctttttctt tttttcttct ttttctccaa tcttgcccga tggctcgaac
    21601 gatttcccca aggaaaaccc gtaactttga agttttagga tgcttccaat ccaatgatgt
    21661 cctatctgta gcaattaaat cttcatcatc attatctata aaatcaatac ttaaccatcc
    21721 tgtcagatag gaatagaaat ggctggattc gctatcagtg aaaaactcgg gcatatttac
    21781 cattctgccg ttggcaaaca aggtaatacc tttcatatta ttctttaaag gtttttccgt
    21841 tgtaatgaac ttaccttgta ttaatccgct gtattcatac tgttcgaatt ttgaagataa
    21901 cgatgaaata ttggttgcta gatcctggta ttcccattcg aattgtgggg taacaagatc
    21961 atattttaga tttccatcaa tgattttggg ttccgagccg ttcaagctta ctttgatttt
    22021 aaaatcctga gccggaaaat caaacaagcg ggaaagatgc tccacataat tatctaacgg
    22081 gtatccttgt tttttcgtca gttcggttaa agttatggtt gttccgtttt cggtattggg
    22141 ttcaacagat tctttctgaa actccggttg ataaatacgc tcactttttt taatctctgc
    22201 ataatccaaa gtaaaagtaa cccgttcgtt tccttggata gtagagattt caattttgtt
    22261 gccaagcccg aataatgcca atttaccaag acctttttta cccgttggaa ttcttccgca
    22321 cggggaggct tgtttttctt cccttctgtt ccgaccgatt ctcaaataaa aatcattgat
    22381 ttcatcgaag ctcattccta taccattatc tttaataacg attttatgct ccggtttatc
    22441 gaataaccta acttccactt ccgtagcaca tgcatcgtag gcattcgcta tcaactcggc
    22501 aatcgcagga acagtgtgcg aatacatctt tacacccaaa tgttcgatta cggttggctc
    22561 gaaactcatt ttcaattttt cttcagacat gttttctcct tttctaacat tcgatattat
    22621 caacaattgc cctgcctatt ttttccgcat acatcggagg aacggcatta ccaatcaaac
    22681 gggctatctt gtccctgctg cccgctttaa agacatagtt acgagggaac gattgcaatg
    22741 ttgcaccttc gcgcagggat aacgccctat cttcctcagg atgggcaaac cttccgttgg
    22801 aaatactaaa gaatttggtc gttatggtag gtgcgggctt atcccaccat agcctaccga
    22861 acgtgtcttt aaaactattg tcttttccga taaaacattc cagctgtaat tcgggaatat
    22921 gcgcaaaagc caaacggttt cctccgtttt taggtatcaa agccaatcgt ttcaaattga
    22981 tgtcagataa tcccgcacaa ctatgcatga aatccgtctc gtcttgatgc cctgccataa
    23041 ttttgggaaa gccgttttcc attcccaaaa catcgcgtac cgtaagccgt ttgcccgaat
    23101 acttgactgg ttccagcttt tctttggtta ttctgtttgc aattaacgta aatcttttac
    23161 ggctttgggg aattccgtaa tcagccgtat tgtgaatacc gaaatggacg gtaaatcctt
    23221 tttcttccaa taactttatg aaattatcaa gcctgctttc tttcattctg ctcagaattc
    23281 ccggtacgtt ctctacaaca acatatccgg gattgaaata ttctacaaac cgctgaaact
    23341 ccaaaagcaa actcttggat ttttccgatt ttcttttgtc tgtttgaata acactccaat
    23401 attgacaagg actacaacct ataagaatca aatcatcatc attctttttt aacccgagtt
    23461 ctttttccaa ggtttcaggc tgcaattcaa aaacatcagc ctgtataaat ttggcattta
    23521 tattggcttc atatgtttct ttgcagctgg gttcgtaatc tatcccggct aaaacctgaa
    23581 taccggcgga ttgcattcca tagctcattc cacctccgcc acagaaaaaa tcaaccgcct
    23641 ttaacttact gtttttcatt tctatttaaa taattagact agtgagaact aactttatat
    23701 aacatataat ttattgctta tcaaaaataa tttgagattt tattaaaatt catgcatcta
    23761 aataagcaga acttaatttc ctccaaacga gcggatgtct tcaaatgcac acttcacaga
    23821 aaggtcgtct gaaacccatt ttcagacgac ctttttccac ttcccaaaac aagaattccc
    23881 accccctccc caaacgtcgc ccgaaccagt aaacttctca tatttcaaca actccttgga
    23941 agcaaaccat gtctggtatc tacctacccc gcctattccc gccccatatc gccgaacgcg
    24001 gcctgttgta ttttcagcag ggcaaggttc tcgatgtccg aaaaacttcc gccgggcatt
    24061 atcgggcgga ggtgtgcggt tcggaaaact attgggtata gttgaagctg gatagtgatt
    24121 tgtatattaa agacgaaggc tgcaattgtc cttatatcta agagtgcaaa cataccttaa
    24181 attactatat tgcataggca aaatacaagc ctataacgaa ttggaaacaa aatgccgtct
    24241 gaaaacatct tcagacggca ttataaaatc tgttcacctt ttcagatgag taatgtacac
    24301 ccttatacaa tttttgctac tatgccccat aaatccacgg ctaaagatat ccttattatg
    24361 tcctatgatt tatcgaaacg acttgtaatc ggcttagcat caagtgccct attcgactta
    24421 tccgaatcgg ataatatatt tagaatggaa ggggcagaaa cctataggca atatcagaga
    24481 gaaaaacaaa accatcccct aaaaaaggcg ttgtctttcc atttattaaa aaacttctgt
    24541 caatcaatga aataaaccca aacgacccaa cgattgggtt tattctttta tccagaaaca
    24601 atccagatac agattacgag tcataactat aggcttaata ttacacgatt ttcattccat
    24661 caaggcggaa aaccgcacaa atactgaaac actatcgatc gatttgtaaa caagcctact
    24721 taagtaactt gcagtcctta tcatttcctt taaaataatc cagcccgtca ctacacgaac
    24781 tggcggactt cttgcaaata aaggttacta gattttcatt catcttaata ataaaaggat
    24841 ttttatcttt atctatggct accgccttca acatgaattt actgtctaag gccccgcgcg
    24901 cgattccatt caaacggata caaaagcctt ctgcctcttt aatcggcaaa cttggccatt
    24961 tggtagatgt ttgtttaaat ctcccattct gcagataaaa cttttccata aaatgtgcat
    25021 tttctaacaa ggctgcccgc actgtattta tctttgcttt ctcaacataa ttgcgatagc
    25081 tcggataaac aattaaagca agtacagaca atatcaagac cactgatatt aattcaacca
    25141 gcgtaaaccc ccgattatca gtcattactt tacttccaat aagaacagat tattcaacat
    25201 atttctttga acagacttac tatcccattc agcagtatgc atatttccca ctctattttt
    25261 tatccgcggg cagcactggt ttggccgggc cttttggtgc gggcgc
//
LOCUS       DAXHSJ010000008        25061 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000008 DAXHSJ010000000
VERSION     DAXHSJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25061
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.008"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..253
                     /locus_tag="ACFM8U_000257"
     CDS             <1..253
                     /locus_tag="ACFM8U_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC6333565.1"
                     /translation="ETKFKSRCFGGRSAVLKALYMATVAATRFEPLIRDFYQRLPSEG
                     KPYKVAVTACMRKLLTISNARMRDYFAENGITENGIRTA"
     gene            301..867
                     /locus_tag="ACFM8U_000258"
                     /pseudo
     CDS             301..867
                     /locus_tag="ACFM8U_000258"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            947..1375
                     /locus_tag="ACFM8U_000259"
     CDS             947..1375
                     /locus_tag="ACFM8U_000259"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6333566.1"
                     /translation="MPSCANCTITNSFANTTSALYYLLQLFAAAGYLWTGAAKLQNLP
                     ASAPLHLITLGGMTGGVMMVWLTAGLWHSGFTKLDYPKLCRIAIPILFAAAVSRAVLM
                     NVNPIFFITIPAILTASVFVLYLFTFVPIFRANAFTDDPE"
     gene            complement(1422..3611)
                     /locus_tag="ACFM8U_000260"
     CDS             complement(1422..3611)
                     /locus_tag="ACFM8U_000260"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC6333567.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDAARILSVQTAFSDEPPLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     VKLIENWAEQGWIETTEAAKPVLRSYHGQASHSEFVLNANQQKASDAIQTALGRFRPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQIAVRADALRVADAMEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQVFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQET"
     gene            3750..4532
                     /locus_tag="ACFM8U_000261"
     CDS             3750..4532
                     /locus_tag="ACFM8U_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC6333568.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAAKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(4945..6879)
                     /locus_tag="ACFM8U_000262"
     CDS             complement(4945..6879)
                     /locus_tag="ACFM8U_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC6333569.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSMGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(6945..8123)
                     /locus_tag="ACFM8U_000263"
     CDS             complement(6945..8123)
                     /locus_tag="ACFM8U_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC6333570.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8349..8738
                     /locus_tag="ACFM8U_000264"
     CDS             8349..8738
                     /locus_tag="ACFM8U_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6333571.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRIRLSEVK
                     STLLMNAQNLERYYRQKGTFGNYDQTKLKQNKYFKITLSKVSPDHFTLQAAPDPMTNE
                     GETCIVTLNDGGTLSAAGSGQDCPDFD"
     gene            9021..10067
                     /gene="adhP"
                     /locus_tag="ACFM8U_000265"
     CDS             9021..10067
                     /gene="adhP"
                     /locus_tag="ACFM8U_000265"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC6333572.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKKTGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(10232..13717)
                     /gene="smc"
                     /locus_tag="ACFM8U_000266"
     CDS             complement(10232..13717)
                     /gene="smc"
                     /locus_tag="ACFM8U_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC6333573.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNRQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELQAEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTRQDEAN
                     RIRRELALKQQQLAHAEQTIAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAVAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDDLSGGIKKSLPVQALLNQIQAQPPFQTALHYWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKAHLDGIASELDNLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIAILQEAAAELEHQQQTAAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQEQGREQYAKVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            13719..13928
                     /locus_tag="ACFM8U_000267"
     CDS             13719..13928
                     /locus_tag="ACFM8U_000267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333574.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APNPGSANADAWRGICRIAPPPGAL"
     gene            13965..14687
                     /gene="lpxH"
                     /locus_tag="ACFM8U_000268"
     CDS             13965..14687
                     /gene="lpxH"
                     /locus_tag="ACFM8U_000268"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC6333575.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAARQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIGKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLATKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            14956..16542
                     /locus_tag="ACFM8U_000269"
     CDS             14956..16542
                     /locus_tag="ACFM8U_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC6333576.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(16711..16914)
                     /locus_tag="ACFM8U_000270"
     CDS             complement(16711..16914)
                     /locus_tag="ACFM8U_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC6333577.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            17030..17527
                     /locus_tag="ACFM8U_000271"
     CDS             17030..17527
                     /locus_tag="ACFM8U_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC6333578.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            17609..18802
                     /locus_tag="ACFM8U_000272"
     CDS             17609..18802
                     /locus_tag="ACFM8U_000272"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC6333579.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(19206..20141)
                     /gene="hemC"
                     /locus_tag="ACFM8U_000273"
     CDS             complement(19206..20141)
                     /gene="hemC"
                     /locus_tag="ACFM8U_000273"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC6333580.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            20260..20775
                     /gene="ybeY"
                     /locus_tag="ACFM8U_000274"
     CDS             20260..20775
                     /gene="ybeY"
                     /locus_tag="ACFM8U_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC6333581.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KSGYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            20777..21601
                     /locus_tag="ACFM8U_000275"
     CDS             20777..21601
                     /locus_tag="ACFM8U_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC6333582.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVIIGSLQFTVARADNRRLHTLMATRVK"
     gene            complement(21744..23123)
                     /gene="nhaC"
                     /locus_tag="ACFM8U_000276"
     CDS             complement(21744..23123)
                     /gene="nhaC"
                     /locus_tag="ACFM8U_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC6333583.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHCYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23414..24637)
                     /locus_tag="ACFM8U_000277"
     CDS             complement(23414..24637)
                     /locus_tag="ACFM8U_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC6333584.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         5738 a         7132 c         6477 g         5714 t
ORIGIN      
        1 ggaaaccaaa ttcaaaagcc gctgctttgg cggaaggtct gcggtgctta aggcactgta
       61 tatggctacc gtggcagcga cacgttttga accgcttatt cgggatttct accaacgcct
      121 gccgtccgag ggtaagccgt ataaggttgc cgttacggca tgtatgcgca aactgctgac
      181 gatatcgaat gcccggatgc gtgattattt tgccgaaaac ggtatcaccg aaaacggtat
      241 ccggacggct tgatttgagt tttggtattt ttacccggcg gggtgaaaaa tactgttgct
      301 atgcgcccgt ttttcgtcgg cgcgacagtg cttgccatac tcggcgcgtt ggtgtttttc
      361 atcagccccg atgccgtcgt cctgcaccgc caaatcttct tagaactcat gctacctgcg
      421 gcatacggcg gttttttgac tgccgctttg ttggactgga cgggtttttc gggcaacctg
      481 aaacctgtcg cgactttgat ggcggcatta ttgcttgtta cggctgtttt attgccgttt
      541 ttaccgcaac ttgccgcatt tttcgtcgcc gcctattggc tggtgttgct gctgttctgc
      601 gcacggctga tttggctaga ccgaaacacc gacaacttcg ccctcttaat gctgcttgcc
      661 gcgttcaccg tttttcagac ggcatacgct gcaaccggcg atttgaacct gttgcgcgcg
      721 caagtgcatt tgaatatggc ggcggtgatg ttcgtatccg tgcgcgtcag tattcttttg
      781 ggcacggaag ccctgaaaga atgccgtctg aaagaccctg tttttattcc aaatatcgtt
      841 tataaaaaca tcgccattac tttcctgctc ttgcacgccg ccgccgaact ttggctgcct
      901 gctcaaaccg ccggttttac tgcgcttgcc gtcggcttca tcctgcttgc caagctgcgc
      961 gaactgcacc atcacgaact ccttcgcaaa cactacgtcc gcactatatt acctgctcca
     1021 actttttgcc gccgcaggct atttgtggac aggcgcggcg aaactgcaaa acctgcccgc
     1081 ctccgcgccc ctgcacctga ttaccctcgg cggtatgacg ggcggcgtga tgatggtgtg
     1141 gctgactgcc ggactgtggc acagcggctt taccaaactt gactacccca aactctgccg
     1201 catcgccatc cccattcttt tcgccgccgc cgtttcgcgc gctgttttaa tgaacgtgaa
     1261 cccgatattc ttcatcacca tccccgcgat tctgaccgcc tccgtgtttg tgctttatct
     1321 ctttacgttc gtacctattt ttcgggcgaa cgcgtttaca gacgatccgg aataaaaatg
     1381 ccgtctgaaa cggttttcag acggcatcag attgccaata atcaagtctc ctgcggatcg
     1441 acatccaccg accatctgat tttgccgtcg cggttttgct gcaacacctg cacccacaaa
     1501 ctcacggcgc ggtgcaaatc ctgtcgagat gtcgattcga ggaaaacttg cgcacgttcg
     1561 cgttcggcaa ggcgcaccat cagcatgggg gcggcgccga actgggaaac gcttgcgggc
     1621 aaaagcgggg cgagggtttc tttggcggcg ttgagaaatt ccatcgcatc ggcaacacgc
     1681 agcgcatcgg cgcggacagc aatctgaaaa ccaaagggcg gcatggcgaa catttgccgc
     1741 tcgttcaatt cgttttcggc gaacacagcg tagtcctgcg ctttgacggc ggcgaagacg
     1801 ggatgttcgg gcagttgggt ctgtatcaac accttgccgg gtttgtcggc acgccccgcc
     1861 ctgccggaca cttgcatcag ctcggcaaac agcctttctg gcgcgcgaaa gtccgcgctg
     1921 tacaggctgc cgtcggcgtt caacacgata acaaggttga gccgcgcaaa atcatggcct
     1981 ttggcgagca tctgcgtgcc gaccagaatg tcgatttcgt tgtcggcgat gcggcggtac
     2041 aaatccgccc agtcattttt gtgcgcggtg ctgtccctgt cgacgcggac gacggcggcc
     2101 ttgggcagga aggcacgcag ggtttcttcg acgcgctgcg tgccgtggcc gacggcggtc
     2161 aggtcttgat tgccgcagtc ggggcatttg tacgggatgg gttcgcggtg gtcgcagtgg
     2221 tggcagcgca gttggcgggc gcgttggtgc agcaccattt tggcggagca gttcgggcag
     2281 ccgaaggtat ggccgcagtc gccgcaaaac agcgcgggcg cgaaaccgcg acggttgagg
     2341 tacaccagcg acataccgcc cgcttcaaaa ttctgtttca aaagctgcaa ggcttgcggc
     2401 gagaaaccgt tgtcgagttt cagacggcct acgttgagaa tgtccacttg cggcagttgc
     2461 gcggcggtat gggcgcgttc ggtcagttgc agcaggcggt acgcgccact ttgcgccttg
     2521 tgccagctct ccaagctggg cgtggcactg cccaacacga cggggcagcc gctctgcttc
     2581 gcccgccaca ccgccaaatc gcgggcatga tagcgcaatt cgttgtcttg tttgaacgag
     2641 ccgtcgtgtt cctcatcgac cacaatcagc ccgacatcat ccatcggcgt gaacactgcc
     2701 agccgcgtgc cgatcaccag cttcgcctgc cctaacatcg cgcgcagata atcctgcgtg
     2761 cgcttgcctg ccgccatctg actgtgcaac acggcggtcg gcacgtcggc aaaacggttt
     2821 tccacccgct tcaaaagctg cggcgtgagg ttgatttcgg gcaacagaaa caacacctgc
     2881 cgcccctgag ccaacacttt cgccatcgca tcgaaataca cttcggtctt gccgctgccg
     2941 gtgatgccgt acagcagaaa cggccggaag cgtcccaatg ccgtctgaat ggcatcggaa
     3001 gctttctgct ggttggcatt caacacaaat tcagagtgcg aagcctgccc gtggtacgac
     3061 cttaaaacgg gtttcgccgc ttccgtcgtt tcaatccaac cctgttccgc ccaattttcg
     3121 attaatttca ccgcctgcgc gtttacctgc ttcaacgctg ccatcgtcat ttcgcccgac
     3181 agcagcgcgt cccacaaagc cgcttttttg ttgaaccgcg ccggcggcgg cgtttgcgcc
     3241 ctgcctgctt cgtttaaagc ataaaacaat ggcggctgcg gcatttccac cgcgcgcgtt
     3301 tccttcaaac cctgcggcag tgcggcaaat actgcctgcc ccgtcggata gtggtaataa
     3361 cgcgacgtaa acgccaacaa atcacgccag ctttcgggca gcggcggctc gtccgaaaag
     3421 gccgtctgaa cgctcaaaat ccgcgccgca tccatatcgg gcgcaatatc cgtttcccac
     3481 accatcccga ccacggtttt attgcggaaa ggcacaagca cccgcgttcc cggaggaagc
     3541 ggatcggaat gggaataagt caaaaggccg tctgaaagcg gcacgtttac ggcgatgcgg
     3601 tggtagatca tacaggcagg caaatcattt ttttgcgtat tttaacaggc ggaagctatt
     3661 ttcagacgat aaattgagcc taggcgcaat gcaggcgcaa cagagatttg atacaattcc
     3721 gcctttccaa cacgcgcaat catctttata tgaaaaccaa gttaatcaaa atcttgaccc
     3781 cctttaccgt cctcccgctg ctggcttgcg ggcaaacgcc cgtttccaat gccaacgccg
     3841 aacccgccgc caaagccgag tccgccggca aatccgttgc cgcctctttg aaagcgcgtt
     3901 tggaaaaaac ctattccgcc caagatttga aagtgttgag cgttagcgaa acaccggtca
     3961 aaggcattta cgaagtcgtc gtcagcggca gacagattat ctacaccgat gccgaaggcg
     4021 gctatatgtt cgtcggcgaa ctcatcaaca tcgacacgcg caaaaacctg accgaagaac
     4081 gcgccgccga tttgaacaaa atcgacttcg cctccctgcc tttggacaaa gccatcaaag
     4141 aagtgcgcgg caacggcaag ctgaaagtcg ccgtcttctc cgaccccgat tgtccgttct
     4201 gcaaacgctt ggaacacgag tttgaaaaaa tgaccgacgt gacggtttac agctttatga
     4261 tgcccattgc cggcctgcac cccgatgccg cgcgcaaggc gcaaatctta tggtgtcagc
     4321 ccgaccgcgc caaagcgtgg acggattgga tgcgtaaagg caaattcccg gtcggcggca
     4381 gcatctgcga caatcccgtc gcggaaacca cttccttggg cgaacaattc ggcttcaacg
     4441 gcacgccgac cctcgtcttc cccaacgggc gcagccaaag cggctacagc ccgatgcccc
     4501 aactggagga aatcatccgc aaaaatcaat aatccatccg caaaaaaggc ttggaaaatt
     4561 ccaagccttt tttgcgggaa atgccgcctg aaaaccaatc cgccctgaaa gttagcaagc
     4621 caacaaacac atcctaaccc attgaatgat tgaaaataat aaattgaaca atacgaagcc
     4681 ccgccgccgt tcccacgcgg gcgggaatct ggaatattga gttaccgtga tttatccgaa
     4741 acaagcaaag ccgaacgaac cgaattcccg tctttacggg aatgacggga ctcgagcgtt
     4801 gtgtgcaatc atcgaacccc cgccgccgtc attcccacgc aagtgggaat ctggaacgta
     4861 gaatctaaga aaccgttttt atccaataag tttctgctcc aaaatgaccg gtcctgcagc
     4921 ttcagacggc atctttgtcc aacctcaatc ctgtgccaat gcgtctatcg gattgagttt
     4981 ggctgcttta ttggcaggca taaagccgaa cgcgatgccg attccggtcg aacaggcgac
     5041 cgcgccgatg acggacatgg cggaaatgtc catcgggaag tcggttacaa aatgattgaa
     5101 cacgaggctg acggcggcgg acaaacccac gccgaccaaa ccgccgatga cgcagattaa
     5161 caccgcctca atcaaaaact gctgcaaaat attgccgcgc cgcgcgccga ttgccatccg
     5221 tatgccgatt tctttggtgc gctcggtaac ggacaccagc atgatgttca tcacgccgat
     5281 gccgccgact accaatgaaa tcagggcgat ggaggaaatc agcagcttca tcgtaccggt
     5341 ggtgctttcg actatctgcc tgatgctgtc gctgttgttc atgaagaaat cttccgtgcc
     5401 gtgccgcgct ttgagcagat cggtcagccc tttttcggca acctgggtat tggcattgtc
     5461 tttgattttg acggtgatgg agttggtgtg gctctcgcct gtgatttggt gcatcaccgt
     5521 cgtatagggc gaccaaagca tcagcacgtc ggaattgccg aaagcgtttt cgtctttttt
     5581 catcacgccg atgacggtca aggggcgttt cctgaacaaa atggttttac ccaacggatc
     5641 cgagtccgca aagagtttgt ctttgacatt ttggtcgatg acgacgacct gcgcgtcttc
     5701 tttcacatcg ttttcgtcaa acagccgccc cgtttccagc ttcagtccgc gcacgtcgaa
     5761 atattgttcg cccacgccgt aaagcgaggc ggtcaggtcg gtattgcggt aagtcagcgt
     5821 gccgccgctc gaagtcatgg gcgtggcgga agcaacgtag ctttgtttgg cgatgatttt
     5881 tgcgtcgtct atggtcaggg ttttaatcct gccgctgcgc ctgtcgccaa agccgcgccc
     5941 ggggaagatg ctgatggtgt tcgtccccat cgaactgatg tcttcgagga tttttttctg
     6001 cgaaccgttg cccagtgcga cgacggagac aaccgaagcg ataccgatga tgatgccgag
     6061 catcgtcaga agcgaacgca ttttgtgcgc caatactgct tgcaccgaca ttctgaaggc
     6121 ttcgacaaac tggtcgtaat aaaacgacca cgaggctttt tcctgaatcc tcccgacatt
     6181 gcttgcgggg atttctggat ttttcgaggt gtcggaaatg atttcgccgt cccggatttc
     6241 gatgacgcgg ttggcattgg cggcgatgcc ggggtcgtgc gtgaccataa tgacggtatg
     6301 cccggcttcg tgcagcttat ggatgatttc catcacgttt ttgccgctgg cggtatcgag
     6361 cgcgccggtc ggctcgtcgg cgaagatgat ttctccgccg ttcatcaggg cgcgggcgat
     6421 ggagacgcgc tgctgctgtc cgcccgagag ttcgccgggc ttgttgccct ctttgcttgc
     6481 caaacccaaa tcctgcaaga gtttgtccgc ccgcgcggaa cgctctttgc cgcccgcgcc
     6541 catatagacg gctggcagcg cgacgttatc ccttgcggtc agcgagctta agaggttgta
     6601 gcgttggaag ataaagccga agcgttcgcg gcgcaatgcc gccagttcgt caggctgcat
     6661 tttggcagtt tcgatgccgt cgattcggta cgaaccggaa ccggcggtat ccaaacaacc
     6721 gagtatgttc atcagcgtgg acttgcccga accggactgc ccgatgatgg cgacaaaatc
     6781 gcccttctct atcgacaggc tgatgtcttt caaaatatgg acgcggttct cgccgctgcc
     6841 gaaatagcgg ttgatgtttt tacattcgat caagctcata atattccgta aatcaataac
     6901 aaatgccgtc tgaaaccgtt tccgtgttca gacggcatat tcgtttatcg gcgcggcggg
     6961 ccgcctaggg cgcgttcgcc gctttcctgt tgctcggcgg cggttatttc ggagatgacc
     7021 actttgtccc cctctttcaa cccgcttttt acttcggtat tcatactgtc tctcataccg
     7081 gtccggattt cgcgttccgc cgccttgccg tccgcaccca acacgcgcac aaacgccttg
     7141 ccgccgcgat ttttcacggt cagcgacgga ataatcagca catttttcac gccgtcgatt
     7201 tcaaccgtat tctgcgtcgt catccccgtg gcgagtttgc cgtccggatt cggcacaaac
     7261 gaacgggcat aatagtagac cgcattggaa gccgtatccg tactgctgtt gtaaccgccc
     7321 gacgacatcg tggtcagccc ggggtcgacg ctgtcgagct tcgccttaat cggcgtatcc
     7381 ggttcggaca aaatcgtaaa cgaaatatcc tgccccgcct tcaccttggt aatatcgccc
     7441 tcggcaatct gcattttgtt caacatcata tccagattcg ccaattggac aatcgtcggc
     7501 gtagactgcg ccgcgttcac agtctgcccc tcttccacga gaatcgccac caccgtgccg
     7561 tccatcgttg cggtaatgcg cgtgtagccc aattccgact cggcggtatt gatggaaatt
     7621 ttgctctgtc tgattaaagc cttcagctcg gcaacattgg ctttggcggc ggcaaacgca
     7681 tcctgcgcgc tttccaaatc ctctttggaa gtcgcgtttt ccttccataa cgccgcctga
     7741 cgcttatatt tcttctccgc gctgcccaat gcaatctgtg ccgacaccag cttcgcctga
     7801 tacgtttcca atttggattt ttccgtattg agcgtattgg tctgcgaggt cgaattgatt
     7861 tccgcaatca aatcgccctt tttaacctgt tgcccgagtt tgacataaag tttcttaatc
     7921 tgccccgatg cctgcgcgcc gaccgatacc aggttggacg gcgaaatctc ccctgttgca
     7981 gaaaccgtcc ggctgatgtc gccgcgcctg accgtttccg taatataagc agcctgcggc
     8041 tcgggcttca gataagacca tccgccccaa accgctgccg ccgcgaccgc cgcaacagcc
     8101 gcccatttca tcatttttgc catgttttca attctctgta tgcgtcaatc ccataaatcg
     8161 gaagcggatt ctaccgcaag tccctgcccc atccaaggcg gcaaactgcc gacaataaaa
     8221 ataaccattt gttttaaatg gcttatacaa tctgctttcc cgaaaatttc aatccgttta
     8281 catattccgc catttccgat acggcgttat aatgtcgccg accgacaaac aaatggaaac
     8341 acattgctat gaaaaacgtt caaaaaggct ttacgctgct cgagctgatg attgccgtcg
     8401 ccatcctcgg catcctgacg ctcatcacct acccttccta taaaacctac atccggcgca
     8461 tccgcctgtc ggaagtcaaa tccaccctgc tgatgaacgc gcaaaacttg gagcgttact
     8521 accgccaaaa agggacgttt ggcaactacg accaaaccaa actgaaacaa aacaaatatt
     8581 tcaagattac cttaagcaaa gtcagccccg accacttcac ccttcaggcc gcccccgatc
     8641 cgatgaccaa cgaaggcgaa acctgcatcg ttacgctcaa cgacggcggc acactgtccg
     8701 ccgccggcag cgggcaggac tgcccggact tcgactaatc ctccgccgaa gcccgcaaac
     8761 ccgtaaagat gccgtccgga cacagcttca gacggtatct ttacgggttt gccgtccggt
     8821 ttttcaaact tgaaacctgc cgcccctcat ccggcaaccg ccccgatgcc gcgcctttcc
     8881 aagctgttat aatataacat ataatctata tttttcaaca acataccact tgacctgtga
     8941 gatgcggaag cgtatgctta cccttatatc ggacaacatt ccgtttacag gtttttttgc
     9001 cgtaactaag gagcgcaatc atgaaaatgc aggcagttgt tgtgaataaa aatgtagcgg
     9061 gcgatgtgga aatagtcgaa cgcgaggttc gcccgttgga atacggcgag gcgttggtcg
     9121 aagtcgaata ttgcggcgtg tgccacaccg acctgcacgt tgcggcaggc gactacggcg
     9181 aaaaaccggg ccgcgtgttg ggacacgagg gcatcggttt ggttaaagaa gttgccgacg
     9241 gtgtgaaaaa tctgaaagtc ggcgaccgcg tcagcatcgc ctggctgttc caaagctgcg
     9301 gctcgtgcga atactgcaat accggccgcg aaaccttgtg ccgctctgta ttgaacgcgg
     9361 gctacaccgc cgacggcggt atggcgaccc actgcatcgt gagtgccgat tacgcggtca
     9421 aagtccctga aggtttggat cctgcgcaag cttccagcat tacttgtgcc ggcgtgacca
     9481 cttataaagc cattaaagtt tccagtgttc gtccgggaca gtggattgcc atctacggcg
     9541 cgggcggttt gggcaacttg ggggtccaat acgcgaaaaa agtattcggc gcacacgttg
     9601 tcgccatcga catcaacgac gacaaactgg cgtttgccaa aaaaaccggc gcggatttgg
     9661 ttgtcaacgc cgccaaagag gacgctgcca aagtgattca ggaaaaaaca ggcggcgcac
     9721 acgctgcggt cgtaaccgcc gtatctgctg ccgcattcaa ctctgccgtg aattgcgtcc
     9781 gcgcaggcgg acgtgtggtt gccatcgggc tgccgccgga atcgatggat ttgtccatcc
     9841 cgcgtttggt tttggacggc atcgaagtgg tcggctcttt ggtcggcacg cgcaaagatt
     9901 tggaagaagc cttccaattc ggcgcggaag gtttggttgt gccgaaagtc caactgcgtg
     9961 ctttggatga agcacccgcc attttccaag aaatgcgcga aggcaaaatc accggccgta
    10021 tggtaatcga tatgaaaaaa gaatgcggct gcggccatca ccactgattt gacgtggcag
    10081 tacacatcga aatgccgtct gaacgctgtt cagacggcat tttttatgga tttggatttg
    10141 attttaatct gttctgtttg aaataccgtc tgaaaaccct tatcgcaaca cgtcattaaa
    10201 aacggcaaga ttcagccgtt ctgcaacccg ttcaaacggc ttccgccatt tccaacgcct
    10261 gcttgatgtc cacggcgacg acgcgcgata cgcctttttc ctgcatggtt acaccgacca
    10321 gctgttccgc catttccatg gtcaggcggt tgtgggagat gtagaggaat tgggtttgcg
    10381 ccgacatttc tttgaccagg ttgcagaaac gtgaggtgtt ggcatcgtcc agcggggcat
    10441 cgacttcgtc caaaaggcag aacggagcag ggttgaggct gaacagggcg aacacgaggc
    10501 tcatggcggt gagggctttt tcgccgccgg aaaggaggtg gatggtgctg tttttcttgc
    10561 cgggcgggcg cgccatgatg gacacgccgg cggtcaggag gtcgtcgcct atcattttga
    10621 gggtagcttc gccaccgccg aacagggtcg ggaagaaagt ttggactttg ccgttgacgg
    10681 catcgaaggt ttctttgaaa cgcgctttgg ttttgtcgtc gatttgggcg atggcttctt
    10741 ccaaaagggt gatggctgcc tgcacgtctt cgctttggct gcggtagtag ccgtcgcgtt
    10801 cgcgcgcttc ttcgagttct tgcagggcgg cgaggttgac cgcgccgagt gcttcgattt
    10861 gttgcgaaag gctgccgatg ctgctgttca atactttcgg cgattctttc gccaacgctt
    10921 cgagcgcgtc caaatcggcg gcgcgttcgg tcaggttttg atggtagcgt ttggcgttga
    10981 tcagggcttc ctgctgctgc aacaaggcgg tttgggtggc ggcctgaagc tgcggcagct
    11041 tggtttgcag ggtttgcact ttagcgtatt gctccctgcc ctgttcctga atctgcgcga
    11101 gtttctcttg cacaacaata tattcttcgt ccaaggtctg tacggcttcg cttaattctt
    11161 caagcttgat gtgctgctcg tcgttttgga actcggtttc ataggcgagg gcaagctctt
    11221 gctggcgttc ctgccagtcc agggtttgct gttcaagctg ggcgatttgc tgccggtaat
    11281 tttgtttttg ctggttgagc ttgtggacgg cgacttcggc aagcccgtat tggcggttgg
    11341 cttccaacag ggcaagctgc gcctgtttca gacgaccttg ctgctcttgg cggctgtgcg
    11401 cggcggtttg ctgctggtgt tcgagttcgg cggcggcttc ctgcaaaatg gcgatgtcgt
    11461 ctgaaagccc gtcggacgtg tgttgcaaca cggtctgctc ttccgccaac tgcgccagtt
    11521 cgcgctcgat gtgttcgcgg cggatttgcc cttggttggt gcgcgccaga agttcggcgg
    11581 cgcgttgctg cgcctgactg tattggcgcg tgtgctgctg ttgttgctgc atcaggtttt
    11641 tgtgctgcac ttcggacgag cgcacggcgg cttccgcctg tttgaacgcg gcttcggcgg
    11701 cggagagttc gggggctagg ttgtccagtt cggatgcgat gccgtcgagg tgcgcttttt
    11761 gggcaatcag gctttcctgc gcgggcttgg catagagcag gacgctgacc ttatcgacct
    11821 gatgaccttc gggcgtgagc cagatttggt gtgcgcccaa atcgttttga tgcgcgaggg
    11881 catagctcaa atcgggcgcg cacaatacgc cgtcgagcca gtagtgcaat gccgtctgaa
    11941 acggcggctg cgcttggatt tggttcagca atgcctgtac gggcagggat tttttgatgc
    12001 cgcctgagag gtcgtctgaa agccatgccg cctgcccctg cggcaaaggc tcgggcggca
    12061 cgaaaccttg cggcacggcg cgggcgtgca ggcgttcggc aagaataacg gacaaggcgt
    12121 gctgccactc ggcgggggcg gtaatgtgtt gccacagttg cggcgcgacg gcgtggtcgg
    12181 ttgcctgcca gaagtcggcg gcttcctgct gttgcgacag gatttgcgac aacgcctgct
    12241 gctgcgcctg taaagtgatg tgttgctgct tcaggctttg gaagcggttt gaggccgtct
    12301 gaaaagcctc gcgggcggcg tgtaaagctt cttcggcggc gatgatttgt tcttcgtaat
    12361 gctcttgccg gctttgcaac aaggcggcgg cttcctgcgc ggcggcggtt tcggcttcgt
    12421 cgggcaggtt cagggcttgg ttttcctgct tcagacggcc tttgcgctct tcgtgcttgg
    12481 caatcgtttg ttcggcatgg gcaagctgct gctgcttcaa cgccagttcg cggcggatgc
    12541 ggtttgcctc gtcctgccgg gtttggaagg cggcgttgag cgtggcttgg gcttcttcca
    12601 attcgggcag acgctcttcg tgttcggcaa cctgcatcgc ccattccgcc aactcggttt
    12661 gcttttcttc tgcctgcaac tcgttttcct caagctgcac gcggatttgc tgctgttctt
    12721 gatggatgcg ctgcatctgc gcctgcgccg cctgcttgtc gcgttcgatg cgttggtgca
    12781 ggttttgctg atggcggatt tgttcttcca aacgggcaat ctgctcgcgc aacacgccgc
    12841 gtttgttgct caattcgtgt accgcctgct gctgcgactg ttcggcggtc tgcaaggcgt
    12901 gtacttcgtc gtttaacgcc tgaacctgcg cggcggtttc gtcctgctgc gcctgcaaag
    12961 actgatgctg cgcggtcgcc ttgtcggcgg ctgcaagcga ttgctgccat tgggcgtaat
    13021 cgagcaaatc ctgttgccgg ttcaactgcg cggtcaggga tttgtagcgt tcggcggttt
    13081 ccgcctgttt ctccagcttt tccacctgac gcgccaactc gttctgcaaa tcgcccaaac
    13141 gctgcaaatg ctcgcgcgtg tctttcagac ggccttccgt ctccttgcgg cgttccttat
    13201 atttggacac gcccgccgct tcctcgatat aggcgcgcaa ctcctccggc cgcgcttcga
    13261 tgatgcgcga aatcatcccc tgctcgataa cggcataacc gcgcgcaccc acgcccgtac
    13321 ccaaaaataa atcggtaatg tcgcggcggc gcacggtctg attgttgatg aaataagtcg
    13381 attcgccctg ccgcgtcagc tgccgcttga tgctcacctc ggcatactgt ccccacgcac
    13441 cctgcaaact gtggtcgctg ttgtcaaaca ccagctccac cgaagccctc ggcgcaggac
    13501 ggcgcgtcgc cgcaccgtta aaaatcacgt cctgcatact ctcgccacga agctgcttcg
    13561 ccgaagcctc acccaacacc cagcgcaccg cgtcaatcac attcgacttg ccgcaaccgt
    13621 tcggcccgat aaccgcgaca agctgccccg gaacatgaat cgtggtcggg tcggtaaaag
    13681 atttgaagcc ggagagtttg atgtgggtca ggcgcataat ggtcggaaaa aataaaaaag
    13741 aagcgtattt taacggaaat cccgccgcac caccccatat cttgccggca aaaccttacc
    13801 gcatcccgca cccgcgatgc cgtctgaagc cttcagacgg catttttaac gcgccgaacc
    13861 ccggttccgc caatgccgat gcttggcgcg gcatttgccg tattgccccg ccgccgggcg
    13921 cgttataatc cgccacgaca tccgcccgat ttggaaacct gcctatgaaa cccgcctatt
    13981 tcatttccga cctgcatttg agcgaaaagc agcccgaact gaccgcgctg ttgctgcgtt
    14041 ttttacgttc ttccgccgcc aggcaggcgc gggcggttta cattttgggc gatttgtttg
    14101 atttttgggt gggcgatgac gaagtttccg agttgaatac ttcggttgcg cgggaaatcg
    14161 ggaaattgtc cgacaaaggc gttgccgtgt tcttcgtcag gggcaaccgc gacttcctga
    14221 ttggtcagga tttttgccgg caggcaggca tgacgctgct gccggattac tcggttttgg
    14281 acttgttcgg ctgcaaaacc ctcatctgcc acggcgacac tctgtgtaca gatgacaggg
    14341 cataccaacg tttccgcaaa atcgtgcatc ggaagcggct gcaaaaactg ttcctgatgc
    14401 tgcccctgaa gtggcgcacg cgccttgcca ccaaaatcag gcgtgtcagc aaaatggaaa
    14461 aacaggtcaa gcccgccgat attatggatg tcaatgccgc ctttaccgcg cggcaggtac
    14521 gcgccttcgg tgcggaaagg ctgatacacg gacacaccca ccgcgagcat atccatcacg
    14581 aaaacggctt tacccgcatc gttttgggcg actggcataa cgactatgct tcaatcctcc
    14641 gcgtagacgg tgacggcgcg gtattcgtgc cgctggaaaa atactgaaaa tgccgtctga
    14701 agcctttcgg gcggcttttt ttacacgccc ctttcccaca ctccctccgc cgcatcatca
    14761 ccatccttaa cccgcattaa tagccattat cgataaaatg cttgaccgat tacataagat
    14821 tacgtaaagt gtgcaaacgc ataacaattg gtcttaccaa tttatctttt aaccaaaata
    14881 tcgggtgtac gggatttgaa cgtctttcca aacctcgacg cgccttatga tgcaaatgcc
    14941 taaaaaggat tcgttatggc acttttcctc agcatattcc ccatcgtcct gctgatttgg
    15001 ctgatggtga aaaaaaacag tatgccctcc tacgtcgcgc tgccgattac cgcagtgctg
    15061 atttacgcca tcaaactttt ctacttcgac gatgcgggca tgctgctcaa cgccaccgcc
    15121 gcttccggcc tcgtcaaaac gctcacgccg attaccgtga ttttcggcgc gattatgttc
    15181 aaccgtatga tggaaaccac gggctgcatc gatgtcatcc gcaaatggct ggcgaccatc
    15241 agcccgaacc ccgtagcgca actgatgatt atcggctggg cttttgcctt tatgattgaa
    15301 ggcgcatccg gcttcggcac gcctgccgcg attgccgcgc cgattctgat gagcttgggc
    15361 ttcaacccgt tgaaagtggc gattttcact ttggtgatga actccgtccc cgtctctttc
    15421 ggcgcggtcg gtacgccgac ctggttcggt ttcgcaccgc tgaacctgag tgccgaagac
    15481 atcctcgcca tcggcaggca gaccggcgta atgcacttct tcgcaggttt cgtcatcccc
    15541 gtcatcggct tgggcttcat cgtaccttgg tctgaaatcc gcaaaaactt gggcttcgtc
    15601 gccattgccg tcttctcctg caccattcct tatgtcgcat tggcgatggt caacgaagaa
    15661 ttcccgtcgc tcgtcgccgg cgcgatcggc ctgatggtgt ccgtattcgc cgccaaccaa
    15721 ggctggggct tgagcaaaga ccacgccaaa gacccgaatg ccgaaaaagt gccgttcgcc
    15781 caagtcgcca aagcactcgc ccctttgggt atgctgatcg gcatgctggt ggttacgcgc
    15841 atcaaacagc tcggcatcaa aggcattttg accagcaaag aagaatggtt cagcttccaa
    15901 ctgccgtttg atttgtccaa aatcaccgtc agcgactccc tgacgattac tttcggcaat
    15961 attttcggac aagatgtcag cgcgtcttac caaacgctgt acgtcccggc ttggattccg
    16021 tttgtgctga ccgtttggat ttgcatcctg ctgtataaaa ccaaattcaa agatgcctgg
    16081 acgatttatg ccgtaacttt caatcaaacc aaaaaaccgc tgcttgccct gatgggcgcg
    16141 ctgattatgg ttcagctgat gctggtcggc ggcgacaatt cgatggtgaa aatcatcggt
    16201 aaggaatttg ccgcaatggc gggcgaacac tgggtttatt tctcgccgta tctgggcgcg
    16261 atcggtgcgt tcttctccgg ctccaacacc gtgtccaacc tgaccttcgg accgattcag
    16321 cagcaaatcg ccttggatac cggcctgtcc gttaccctga ttctggcgtt gcagtccgtc
    16381 ggcggcgcga tgggcaatat ggtgtgcctc aacaacatca tcgccgtatg taccgtattg
    16441 gacgtgaaaa attccgaagg tgcgattatc aagaaaaccg ttatcccgat ggcgatttac
    16501 ggcgtgattg ccgtcgtcgc ggcaatgatt ttcttcctct aggcgaaacg ccaccatcca
    16561 tagcggcagc acgcggggaa cggaaaccgc cgttccccgt ccgggcggcg cgtcgggcag
    16621 aatcccggtg ccggaacaag cccgccccgc caaacaaatg ccgtctgaaa ccggaaaagg
    16681 cttcagacgg catttttccg ccgcttgcgt tcaggcgtaa tgatgatgcg gcatcttgcc
    16741 gtcaaggcgg tagcgcgtgc cgcaatacgg gcaggcaacg ctgcccgatt ctccttcgca
    16801 caaaggcaaa aacactctcg gatgcccgtt ccactgctcg ttgtcgggtc ccgagcaata
    16861 cagcggcaga ttttccggca acacggaaat ttcctgcaga ttcagattgt ccatttgatt
    16921 ttcctttgcg tggttggcgt gcgcccattt tacgccatcg gcaggctaaa ggatattttc
    16981 ggcgcaaagc cgcaatccgc tataatcccc acttttcaga cggcatacca tgactgcgct
    17041 tacccttccg gaagacatcc gccaacaaga gccatccgcc ctgctctata ccctcgtttc
    17101 cgcctacctc gaacacaccg cccaaaccgg cgacgaatcc ctctcctgcc tgtccgacga
    17161 ccagcacacg ctgaccgcat tctgctacct cgacagccaa gtcgaagaag gcggcttcgt
    17221 gcaactcatc gcctccggtt acggcgaata tattttccgc aacccgcttg ccgacagcct
    17281 gcgccgctgg aaaatcaaag ccgtgccgaa agtcttggac aaagccaaag ccctctacga
    17341 acaacacggc aaaaccatcg aaacgctcgc cgacggaggc gcagacatcg aatccctgcg
    17401 caaacagttc cccgactttg aagaatggga cggcgcatac tacgaagccg ccgaacaaga
    17461 cctgcccctg cttgcagaac acatacagtc aaactgggaa accttcgccc atatcgggca
    17521 ggcgtgattg cgtctgtttc cagcccgtgt aaaacagcgt aaaatcggca aacccgaatg
    17581 accttccgtc cccatcaagg agcaagctat gttcttcaag cacatcgaag ccgcccccgc
    17641 cgatccgatt ctcggtttgg gcgaagcgtt caaagccgaa acccgccccg aaaaagtcaa
    17701 cctcggcatc ggcgtttata aagacgcatc cggcgcgaca cccctcgtca aagccgtcaa
    17761 agaagccgaa aaacgcctgt tggaaagcga aaccaccaaa aactacctca ccatcgacgg
    17821 cgttgccgac tacaacgcgc aaacccaaat cctgctgttc ggcaaagacc acgaaatcat
    17881 cgccagccgt cgcgccaaaa cagcgcaaag cctcggcggt acgggcgcat tgcgtattgc
    17941 ggccgagttt gccaaacgcc agttgaacgc gcaaaccatc tggatttcca atccgacttg
    18001 gcccaaccac aacgccatcg ccaaagcggt cggtatccaa gacaaacctt atcgttacta
    18061 tgatgccgcc aaacacggtt tggattggga cggcatgatt gaggacttga gccaagcgca
    18121 aaaaggcgac atcgtcctgc tgcacggctg ctgccacaat cctaccggca tcgaccctac
    18181 gcccgaacaa tgggaaactt tggcaaaact ttctgccgaa aaaggctggt tgccgctgtt
    18241 tgactttgcc taccaaggct tcggcaatgg tttggaagaa gatgcctacg gcttgcgcgt
    18301 gttcttgaaa cacaatacag aattgctgat tgccagctct tattccaaaa acttcggtat
    18361 gtacaacgag cgcgtcggcg cgttcacttt ggtggccgaa gatgaagaaa cagcagcccg
    18421 cgcccacagc caagtcaaaa ccatcatccg taccttgtat tccaacccgg cttcacacgg
    18481 tgcgaacacc attgcgctgg tgttgaaaaa tgatgatttg aaagcacaat ggattgccga
    18541 actcgatgaa atgcgcggcc gcatcaaagc catgcgccaa aaatttgtcg agttgctcaa
    18601 agccaaaggt gcaagccaag actttgattt cattatcgaa caaaacggta tgttctcttt
    18661 cagcggtctg acccccgaac aagttgaccg tttgaaaaac gagtttgcca tttatgccgt
    18721 ccgctccggc cgcatcaacg tcgccggcat taccgacgac aacatcgatt atctgtgcga
    18781 aagcatcgtg aaagtcttgt aaaacaaaag cgcatacgaa atgccgtctg aaccttaacc
    18841 agttcagacg gcatctgttt tttgttaaaa aacaataaac cataacaatt tcaaacaagc
    18901 taaaaaacat ctataaaatc aacccaatat gatgaactca aataaaaaat acgaatatca
    18961 tcattctcaa attcaaaatt tgaacgccac agtattatcc gaagccacaa aaacctttcc
    19021 gccgtcattc ccgcgcaggc gggaatccgg gaacccaatg ctgcaagaat tgattagaaa
    19081 caactgaaac cgagcaaact ggattcccgc ctgcgcggga atgacgaaat aaggcgagta
    19141 aaactgatga aacaaattga ttaaaaacaa taacttatat tattttattt aaattttcga
    19201 taaaatcaat tttccgtatt caatactgct ccaatcaatt cccgcgcacc gtcgtccgcc
    19261 aatttcttag cgacagcgcg tccgagcgcg tcggcatatt cggcaggggc ttgcgcgtcc
    19321 gcccgcaaca caaccgaacc gtcgggatga ccgaccaagc cgcgcaaggt cagcaagccg
    19381 ttttcttccg tgcaatatgc cgccagcggc acttggcagc ttccgcccaa agcgcgcgcc
    19441 aacgcgcgtt cagccgtaac gcaggcgtgt gtggtatcgt ggttcaacgg ctttaagact
    19501 tcatacaaat cttcgcgatg cgctgcaatt tcaataccca atgcaccttg tccggcggca
    19561 ggcagactgt cagattccga caaaatcatg cggatgcgtt cgtccaattc cagacgctgc
    19621 aaaccggcgg cggcaaggat aattgcgtcg tattcgccgt tatcgagttt ggacaaacgg
    19681 gtttgcacat tgccgcgcaa aggtttgata accaaatgcg gatagcgcgc acgcaactgg
    19741 gcttcgcggc gcaggctgga tgtaccgacg acagcgcctt tgggcatttc ttccaaacgc
    19801 gcgtattggt tggacacaaa cgcgtcaaac ggattggcgc gttcgccgat ggcggcaagc
    19861 gcgaaacctt caggcaaatc catcggcacg tccttaatcg aatgcaccgc caaatcggcg
    19921 cgcccgtcat ataaagcctg ttccaactct ttgacaaaca agcctttacc gccgactttt
    19981 gacaaagttt tgtccaaaat ctgatcgccg cgcgtggtca tgcccaaaat ctcgacttcg
    20041 caatcgggat acagagcctt cagacggcct tggatatgct ttgcctgcca catagcaagc
    20101 aggctttcgc ggctggcgat gacgagtttt ttcgggttca tgttcaatgc gttcaaagaa
    20161 tggaaacggc gtgaagtcta tcatattttg aacgtttctt cagacggcac cgtcaggtct
    20221 atgttatatt tacacccaca aaacacgaaa cccgccacaa tgaaacgcac caaaaaatac
    20281 cctttcttaa cgttgcagcg gcaacgtttc catttgaact ttgaaaacgc ctcttccgcc
    20341 gccggcatcc cagccgaacg cgatttctac cgctgggcgt ggtctgcctt gaaaagcgga
    20401 taccgccgcg ccgacatcag cctgattctt ctggacgaag aagaagcccg agcctacaac
    20461 cgcgactacc gcggcaaaga ttacgccacc aatgtattga gtttcgcgct caacgaaggc
    20521 gaaatcctgc cctgccaagt ttcggaaaaa ctgtacggcg atttgattat ctgcccgcaa
    20581 gtggttttga aagaagccgc cgaacagggc aaaacgcccg aacggcattt tgcccacctg
    20641 accatacacg gcgttttgca cctgatgggc tacgaccaca tcgaagacga tgaagccgaa
    20701 ataatggaag ccgaagaaat ccgcctgatg ctggcggcag gcttccccaa cccctaccaa
    20761 gaggacgaat attaaaatgg acggtacgca gtcgaaaccc aagtttttcg aacgcctgat
    20821 ctcccgactt gccggcgagc ccgactccgc cgaagacgta ttgaccctgc tgcgccaagc
    20881 gcacgagcag gaagtttttg atgcggatac gcttttaagg ttggaaaaag tcctcgattt
    20941 ttccgatttg gaagtgcgcg atgccatgat tacgcgcagc cgtatgaacg ttttaaaaga
    21001 aaacgacagc atcgaacgca tcaccgccta cgttatcgat accgcccatt cgcgcttccc
    21061 cgtcatcggt gaagacaaag acgaagtttt gggtattttg cacgccaaag acctgctcaa
    21121 atatatgttc aaccccgagc agttccacct caaatcgata ttgcgccctg ccgtcttcgt
    21181 ccccgaaggc aaatcgctga ccgccctttt aaaagagttc cgcgaacagc gcaaccatat
    21241 ggcgatcgtc atcgacgaat acggcggcac gtcgggtttg gtaacttttg aagacatcat
    21301 cgagcaaatc gtcggcgaca tcgaagatga gtttgacgaa gacgaaagcg cggacaacat
    21361 ccacgccgtt tcttccgaac gctggcgcat ccacgcggct accgaaatcg aagacatcaa
    21421 caccttcttc ggtacggaat acagcagcga agaagccgac accattggcg gcctggtcat
    21481 tcaagagttg ggacatctgc ccgtgcgcgg cgaaaaagtc attatcggca gcctgcaatt
    21541 taccgtcgca cgcgccgaca accgccgcct gcatacgctg atggcgaccc gcgtgaagta
    21601 agcagagcct gcccgcaccg ccgtttctgc acagtttagg atgacggtac ggacgttttc
    21661 tgtttcaatc ctccccatcc gccaaacata aaaatgccgt ctgaaaccgg aaaacaggtt
    21721 cagacggcat ttcgcttatc cgcttatttt ttgctcaaag tcagccccgt ccagccgaat
    21781 aacagggtta aagccaaact caaatagcag aaaaaggcat agggcagata ttcccagacc
    21841 ggcacaccca gcgcatggct gataaatacg ccgcacacgc tccacggaac aagcgggttg
    21901 atgaccgtac ccgcgtcttc gagcgtacgc gagaggttgc gggaatgtag tccgagctta
    21961 tcgtaaacgg gtttgaacgt ttcacccgac aacaaaatac tcaaatattg ctcgccgatc
    22021 aggaaattaa ccccgaccga agtcatggca acgctgaatg tggcacgtcc ggcattcgtc
    22081 aaaaagctgc ggacggcatc cagcagggaa ggaatcgcgc ccagcgcaaa cagcagcccg
    22141 cccaaactca tcccgagaat cacgatggtt tgcgtgaaaa acatactttc caagccgccg
    22201 cgcgaaatga gtttggcgat gtctttaaac gcttcgcctt cgagtttgta gccgccgtaa
    22261 aaccacgcgc cgagctgacg cagatcgggc gtgctgtgca gatacgttac ggcaacggcg
    22321 gcaatgacgg taaagagcat agcgaccacg gcattgacgc gcatcaatgc caaaacgacc
    22381 aacagcgcaa acggaatcag cgaatagcag tgcaccaatc ccgtggcttc aagctggctg
    22441 cggaaggatt cgacgctgtt caaatcctgc gcggcgacgc tgggaagaag ccacagcatc
    22501 agtgccgcac tgatgagcca cgcgggaatg gtggtgtaca tcatattttt gatgtgttca
    22561 aacaggtcga taccgacaat ggacgcggaa atgcccgtcg tatcggaaag cggggacatt
    22621 ttgtcgccaa aaaatgcgcc cgaaacaatc gcgcccgccg tcatcgccat atcggcctga
    22681 aacgccgccg ccatacccat aaaggcaacg ccgacagtgg cgcaggtggt caggctgctg
    22741 ccgatggaca cgccgatgac ggaacacagc gcgaaggcgg aaaaataaaa ataagtcggg
    22801 gaaatcagtc cgaaaccgta atacatcagc gtcggaatcg cgccgctcat catcagcgcg
    22861 ctgaccatca gcccgatgaa gaaaaacagg taaatcgcgc ccataccctg attcaacgcg
    22921 cctatcatgc cctgctgcat atcgttgtat ttcaaaccgc gcgccaagcc gtacaaaatc
    22981 agcacgacga tggcggcaat aatggacata tgcggcagcc actccaatga aatgatggta
    23041 tagcccatcg cggcaatcag agcgacgacg acggcaagtg cctcaccgcg cggcatatcg
    23101 agtaaggatt tgaaagcgaa catgattctt tgcccgattt gatataaagt taacggattc
    23161 taacacacca taccgaatcc gcccgacgtt ttcagacggc attttagcgt tttacaaaag
    23221 ccggacagaa acaaaacggc aattttctca atttaaatta atttttttga aatataattt
    23281 tttatttttt tgtttgttga catataagac ttttatggat tgaagtatta aaacctcggc
    23341 acacacgccg caaacaccgg tacgcaaaaa tgccgtctga aagatgttca gacgacattt
    23401 ctcaagaaaa agattatttg tccgccctat atccgtacac cgcaaaaaac acgatataca
    23461 gataacagat gacggaaacg acaaacgaag acatcagggt gtaagtatct gccacccagc
    23521 cctgcacgac aggaacgacc gcaccgccga caatcgcggt acacagtaca ccggaagccg
    23581 cgttggtaaa ttttcccaat cctttggttg ccaaagagaa aatcgtcggg aacataatcg
    23641 agttgaaaaa accgatggca agcagcgacc acatcgccac atccgcattg ccgctacccg
    23701 tcgccatcgc gacggcaagc agtacgaccg cagccgatgc gttaaacgcc aaataacggt
    23761 tgggcgcgaa tttcgccatc accgccgaac cgaggaaacg tccgaccatc gcgccgcccc
    23821 aatagaacga caggtaatgc gcggcagaag catgatccag ccctttcaga taacccaata
    23881 cgttgaccat caacgaaccg atagacacct ccgcgccgac atagcagaaa atgcctgccg
    23941 taccgaacac gagatgtttg tattgccata cgctggtttt gccgtcgtgg ttgtgcgcgc
    24001 tttcctcggc ggcaattttg cgcgcgtcgg gcagccggat cattttcacg aaaacggcaa
    24061 ggataatcag cagccccgcc agtcccaaat agggaatctg tacggaagaa atctgttccg
    24121 ccttgctgac ggtttgggtt gcgtccgcca gaatcaggaa cgcgccgatt tgcggcgcaa
    24181 tggtcgtacc caaagcgtta aacgcctgaa ccagcgtcag tgttgccgat tccttgccgg
    24241 gtttcgccag cagggtaaca taaggattac cggcgacctg aagcagcgtt acgccggaag
    24301 ccaaaataaa caacgcgccc aaaaataccg cgtaagaatg gctacccgca gcaggataaa
    24361 acagcaggca tccgaccgcc gtcagcagaa agccgccgat aacgccgttt ttgtagccga
    24421 ctttgccgac aaaagccccc atcgggatgg acatcaccgc ataggcggta aagaaacaga
    24481 attggatcag catcgcctga acgtaagaca ggtcgaaaat ttctttcaaa tgagggataa
    24541 ggatgtcgtt catgcaggta ataaaaccca tcatgaagaa cagcgtggtc aagacgacca
    24601 atggggacgt atgattgttt tgtgattgcg cagacatgat ttgcctttcg tggtgtcgga
    24661 atattacccg tttcaagaaa atagaaattg acatttctta acggcaatgc aaatcatact
    24721 gatatttgaa cattttgcaa actttatcgg atgcaggcaa tgcgccgatc tgaaattcaa
    24781 gcagcagaaa ttaatatttt acagttgctt gcatagattt taatagattt taaaaaattc
    24841 cggcggactt gcaaaaaccg ttcccaataa aatgaaaatc cgccgccgta tccggaaaat
    24901 tcggaaatgc cgcatcgtgg catcaaaacc ggagcggaaa gaatgtcgta tgcccgacaa
    24961 aaaaggggca tacggctgtg ccgtattttt tgcaccgtcc tccgcccttc ggcgggggcg
    25021 tgaaaatata gtggattaac aaaaatcagg acaaggcgac g
//
LOCUS       DAXHSJ010000009        24735 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000009 DAXHSJ010000000
VERSION     DAXHSJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24735
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.009"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            141..1538
                     /gene="aspA"
                     /locus_tag="ACFM8U_000278"
     CDS             141..1538
                     /gene="aspA"
                     /locus_tag="ACFM8U_000278"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC6333585.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVISNLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLAEKCVKGI
                     TVNREICERYVFNSIGLITYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1735..2610)
                     /locus_tag="ACFM8U_000279"
     CDS             complement(1735..2610)
                     /locus_tag="ACFM8U_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC6333586.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAVLLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFLLAGVLMFACLFAKSVRWRAESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2612..3334)
                     /locus_tag="ACFM8U_000280"
     CDS             complement(2612..3334)
                     /locus_tag="ACFM8U_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC6333587.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGCNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3357..3836)
                     /gene="ybaK"
                     /locus_tag="ACFM8U_000281"
     CDS             complement(3357..3836)
                     /gene="ybaK"
                     /locus_tag="ACFM8U_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC6333588.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3911..4273
                     /locus_tag="ACFM8U_000282"
     CDS             3911..4273
                     /locus_tag="ACFM8U_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6333589.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKKDAATA"
     gene            4308..5171
                     /locus_tag="ACFM8U_000283"
     CDS             4308..5171
                     /locus_tag="ACFM8U_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC6333590.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVDAEL
                     EASAEPLAAQEPSLVFTAECVSDGICTVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVQDSENFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            5269..6228
                     /gene="ttcA"
                     /locus_tag="ACFM8U_000284"
     CDS             5269..6228
                     /gene="ttcA"
                     /locus_tag="ACFM8U_000284"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC6333591.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(6362..6916)
                     /locus_tag="ACFM8U_000285"
     CDS             complement(6362..6916)
                     /locus_tag="ACFM8U_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6333592.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIDGFVIQGGGFTEDLVQKASDKAVANESGNGLKN
                     TAGTVAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNIPVQPVKIRRVVAE"
     gene            complement(6981..7898)
                     /locus_tag="ACFM8U_000286"
     CDS             complement(6981..7898)
                     /locus_tag="ACFM8U_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6333593.1"
                     /translation="MKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEFGEI
                     GIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAHAAS
                     VIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLVASKTDLCT
                     TEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMGFQS
                     QGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGANQV
                     SWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            complement(7959..8084)
                     /locus_tag="ACFM8U_000287"
     CDS             complement(7959..8084)
                     /locus_tag="ACFM8U_000287"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333594.1"
                     /translation="MNLSVVWETVLNVIPAMSADISNLLEQLSIDEEHDNSCHHF"
     gene            complement(8081..8353)
                     /locus_tag="ACFM8U_000288"
     CDS             complement(8081..8353)
                     /locus_tag="ACFM8U_000288"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HFC6333595.1"
                     /translation="MKMQKELSVYLKQILQAAQYIRLYTDKMDYGQFSADTKTVQAVV
                     FNLFLIGENATHILKSYPEFAEETKYLNWIGMRGYCAIGLPMATLK"
     gene            complement(8350..8643)
                     /locus_tag="ACFM8U_000289"
     CDS             complement(8350..8643)
                     /locus_tag="ACFM8U_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFC6333596.1"
                     /translation="MKPSLLLQSRKKEILAVFGKYPLICNPRVFGSVSRGDDTENSDI
                     DLLVDAKTGTTLLDLGGLQEELQKLLGIKVDLLTPDDISAHFRDKVLTEAVAL"
     gene            complement(8688..9164)
                     /locus_tag="ACFM8U_000290"
     CDS             complement(8688..9164)
                     /locus_tag="ACFM8U_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC6333597.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQQ"
     gene            complement(9235..9690)
                     /locus_tag="ACFM8U_000291"
     CDS             complement(9235..9690)
                     /locus_tag="ACFM8U_000291"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC6333598.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKYGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            complement(9815..10930)
                     /locus_tag="ACFM8U_000292"
     CDS             complement(9815..10930)
                     /locus_tag="ACFM8U_000292"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC6333599.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            complement(11044..12141)
                     /locus_tag="ACFM8U_000293"
     CDS             complement(11044..12141)
                     /locus_tag="ACFM8U_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC6333600.1"
                     /translation="MNQTFTLPDTRPYPQNQIKNHLLLNAYQLAHNSSQASRKLSFGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLKNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            complement(12223..13806)
                     /locus_tag="ACFM8U_000294"
     CDS             complement(12223..13806)
                     /locus_tag="ACFM8U_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HFC6333601.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLKPAAAAKAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEALNDPIYVNGFIEWANRHRLAYIGDTDLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDNIRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEAFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQAVIDAVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            complement(13954..14166)
                     /locus_tag="ACFM8U_000295"
     CDS             complement(13954..14166)
                     /locus_tag="ACFM8U_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC6333602.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            14512..14667
                     /locus_tag="ACFM8U_000296"
                     /pseudo
     CDS             14512..14667
                     /locus_tag="ACFM8U_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            14797..16233
                     /locus_tag="ACFM8U_000297"
     CDS             14797..16233
                     /locus_tag="ACFM8U_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC6333603.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSAQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDRENTRRFWLGCGIAASLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLSFVVFNTASPD
                     DAGAVFNALFANASGWNAPQQADMLLLASFAAVMLLYPYLQRAFDGVVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            16244..17227
                     /gene="patB"
                     /locus_tag="ACFM8U_000298"
     CDS             16244..17227
                     /gene="patB"
                     /locus_tag="ACFM8U_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC6333604.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            17224..18417
                     /locus_tag="ACFM8U_000299"
     CDS             17224..18417
                     /locus_tag="ACFM8U_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC6333605.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKIRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRYNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRIA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(18477..18653)
                     /locus_tag="ACFM8U_000300"
     CDS             complement(18477..18653)
                     /locus_tag="ACFM8U_000300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333606.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAVCRRFAPVLPMLEANAGCV
                     RQTVRGVAMPSEAI"
     gene            18704..20257
                     /locus_tag="ACFM8U_000301"
     CDS             18704..20257
                     /locus_tag="ACFM8U_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC6333607.1"
                     /translation="MNRTYANFYGMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTGQSRPDGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(20348..22375)
                     /locus_tag="ACFM8U_000302"
     CDS             complement(20348..22375)
                     /locus_tag="ACFM8U_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC6333608.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFGWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            22664..24670
                     /locus_tag="ACFM8U_000303"
     CDS             22664..24670
                     /locus_tag="ACFM8U_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC6333609.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLHQQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
BASE COUNT         5978 a         7175 c         5940 g         5642 t
ORIGIN      
        1 ccgaaaacaa cgtttcccgt tttcagacgg catacgatat ggacaatttc atccggccgg
       61 tatagtgttc gtcctgcggc ggcatctttc cgatggcgca ttttttcaat attaattatc
      121 cgacttacaa aggtaaaaac atgactgtcc gtatcgaaca cgatttattg ggcgaccgcg
      181 agattcccgc cgaagtgtat tggggcatcc acactctgcg cgccattgaa aactttaaaa
      241 tctccacaca aaaaatttcc gacgtgccgc agtttgtccg cagtatggtg atggtgaaaa
      301 aagcaaccgc gcaggcaaac ggcgaattgg gtgcagtaaa acccgaaatc gccgccgcca
      361 ttgaaaaggc ttgcgacgaa gttctgctga acaaccgctg cctcgaccaa ttcccctccg
      421 acgtgtatca gggcggtgcg ggaacttcgg tcaatatgaa caccaacgaa gtcatctcca
      481 acctcgcatt ggaagccttg ggatacgaaa aaggccgcta cgacatcgtc aatccgatgg
      541 accatgtcaa cgccagccaa tcgaccaacg atgcctatcc cacgggcttc cgccttgccg
      601 tgtattacag catcggcgaa ttgctcgaca aactggccgt cctgaaaaac gcctttgccg
      661 ccaaagccga agagtttaaa gacgttttga aaatgggccg cacccagctt caagatgccg
      721 taccgatgac tacaggacaa gaattccaat ctttccaagt gttgttggaa gaagaaatcc
      781 tcaacctcga ccgcacgcgc caactgctgt tggaagtcaa tttgggcgca acggcaatcg
      841 gcacgggcgt gaacacgccc aaaggctacg ccgaactggt ggtcaaaaaa ctctccgaag
      901 tcagcggctt gccttgcaaa ctgaccgaaa acctgattga ggcgacctcc gactgcggcg
      961 catatgtgat ggtacacggc gcattgaagc gcacggcggt caaactctcc aaaatctgca
     1021 acgacttgcg cctgctctct tccggcccgc gtgccggctt gaaagaaatc aacctgcccg
     1081 aattacaggc cggttcttcc attatgcctg ccaaagtcaa tcccgtgatt cccgaagtgg
     1141 tcaaccaagt ctgcttcaaa gtcatcggca acgacaccac catcactttc gccgccgaag
     1201 caggtcagtt gcaattaaac gtaatggagc cggtcatcgc ccaatgtatg tttgaaacga
     1261 tttccctctt gggcaatgct gcggtcaacc tcgccgaaaa atgcgtcaaa ggcattacgg
     1321 tcaaccgcga aatctgcgag cgttacgttt tcaactccat cggtttgatc acttatctga
     1381 atccgtacat cggccaccac aacggcgact tggtcggcaa aatctgcgcc cagaccggca
     1441 aaggcgtacg cgaggttgtt ttggaacgcg gcctgttgag cgaagaagaa ctcaaccgca
     1501 tcctctctcc tgaaaacctg atgaaccccc atctgtaacg ttgcaggcaa atgccgtctg
     1561 aagcagggat gctgcttcag acggcatttt cggcattcaa ggctgtttta gccgccgccc
     1621 gattccggcg gtttgtcgga aaaagccagt tatttccgtt cgggaaaaat ccagtttatt
     1681 gacaaaaaaa caattactta aaacgcgttt gcgcggtatg cggacacggc gggattatat
     1741 gccgaacagt gccttgacct ccgcccaata aaacaggcag gcgatggaac acagtgccaa
     1801 tacgattccc gccgtattga ttgtgttgat tttttcctta aaaaacagtg cgcccgaaag
     1861 cgtacccaaa acaatcacac cgatattcat accggcaaaa accaaggtcg gattgtcctt
     1921 catcatttgg tgcgcggtga tgtaggttac gatattcata aaattcaaac cgcccaagag
     1981 tatgccgccg accacactct ctgcgcgcca tctgaccgat ttggcaaaca ggcaggcaaa
     2041 catcagcaca cccgccagca aaaatgcaac cagcaggttg cccgcaaatg ccgttccgct
     2101 tttggcaagc tgtttgaaca ggatatcgat aatgccgtaa cctgcccaca cgcccagcag
     2161 caataccgcc tgccgccacg cgcttcctga ttttttgcca ccgctgtgtt tccaaagcag
     2221 gcagaacagt gcggcaaatg cgaggcacag cccgattagt ctgccttcgc tgagtttttc
     2281 gccaaacagc gtcaaggcgg caacaatcgg caaaaacagc gacaaacgct gcgccgcgtc
     2341 ggatttgacg ataccggcgg attctacgga tttgcccata atgacaaata cggacggcag
     2401 cagcacgccc aatgcggcaa acagccacca tgtcggcaaa aatgcgccga tattgccgac
     2461 atccggcttc aataccagca gggtcagtat gaccgcgacc acataattga cggcgaccgc
     2521 ctgcgcgatg tcgatttttt tcttccttgc cattttcagc aaaatggaaa ccgacacgct
     2581 gaacacgatg ctgattaaca ggtaagccat actatatcca tttctcgggt gcaaccgccc
     2641 tgccgccgtt gaagcggata aagccggcga tgatgcggta gaaataccag acgttcgttg
     2701 caataataat cagcacgccg atacccaaaa gggcagtcag cgcacccaaa atataaagcc
     2761 aaaatgtgcg ccaaaaggtt ttaatcaggt attcggtatg tgcggcatag acaatgctgt
     2821 ccaaactgtt ccgtttcata taggcgagaa tcacgccgac tatgggcata taggcgaaaa
     2881 tcacgccgac tatgggcatg aacagcatga ttgcacccaa accgaacagg atgtaggcgg
     2941 cgaggacata gttgcacccg cttccgccgc cggacggttc gtaagtatgg cacgaacgtg
     3001 atgcctgccg ttcaaacgcc tgtctgaacg cctgttcgcg gcgcatctgt tcgcggtaaa
     3061 actgctccct gcgcgcctgt tcgcggcgga atgcctcttc ctgccgcccg cgttcctcat
     3121 gccgtctgaa ggacgcgtcg tattgcatcc gtttcgacag gtcggaaagc gtatcgtaag
     3181 cccgttggat ttctttgaac ttttcttccg ccttcggatt gcccggattg cggtcgggat
     3241 gatatttcat cgccagcttg cggtaggcgc gtttgatttc gtccgctccc gcctgcggcg
     3301 acacgcccaa tacggcatac aaatctttgt ccatattgtt cctttatact ttcccgtcaa
     3361 accgccgcct gtattgtttt cgggttcaaa atatttaaat ctccgggacg gatgccgata
     3421 atgaacccgc gttttccgcc gttgatatag atggtttcca aatccatcac cgactgttcg
     3481 acgtaaatat ccaactttgt ccggatgccg aacggcgttg tgccgccgac cagatagccc
     3541 gtccacttgt ttgcctgtac gggcgtggcg ggttcgatgt gtttcgcacc caaatgccgc
     3601 gccagattgc gggttgaaat ctgcttgtcg ccgtgcatca agacaatcag ccccttaccg
     3661 ttttcatctt gcaaaacaat ggttttaatg accaagtgtt cgtctttgcc gaatagtcgg
     3721 gcaaactgcg ccgtgccgcc gtgttcctca taggcatagg taaaaggttc aaattcgatg
     3781 ccgttttcac gcaaaacgcg cacggcaggt gttatcggat aagtgtgttt actcattttt
     3841 cgtcatgcaa aacatggaaa tcggtcaagc gcaatgttac catatatccc ctttatcgaa
     3901 ggaaaccgat atggatatcc gatatttcgg cacaacgccc cgctattccg aagccgtcgg
     3961 cgcaaacggc ttgattttcc tctccggcat ggttcccgaa aacggtgaaa cggctgccga
     4021 acagactgcc gacgtgcttg cccaaatcga ccgctggctg gcggaatgcg gctcggacaa
     4081 agcgcacgtt ttggatgcgg tcatctatct gcgcgatatg ggcgactatg cggaaatgaa
     4141 cggcgtgtgg gacgcttggg ttgccgccgg cagaacgccc gcccgcgcct gcgtggaagc
     4201 gcggctcgcc cgtccggagt ggcgcgtcga aatcaaaata accgccgtga agaaagatgc
     4261 ggcaaccgca taaacagatt tcctttatat ttcagagtac gcaaatgatg aaaaccttac
     4321 tgcccttttt cggcgtggta cgcgtcagcg gcgaagaccg ccaaaccttc ctgcacggac
     4381 agctttccaa cgacatcaat aatcttcaga ccgggcaggc gtgttatgcg acttacaaca
     4441 cgcccaaagg ccgtgtcatc gccaatatga ttgtcgtcaa ccgaggcggc gatttgctgc
     4501 tgattatggc gcaagacttg cttgaagcaa cggtcaaacg tttgcggatg tttgtgttgc
     4561 gtgccaaagc cgtttttgaa atccttgaag attacgccgt cgacgcggaa ttggaagcat
     4621 ccgccgaacc gcttgccgcg caagaaccca gtctcgtctt taccgccgaa tgcgtttcag
     4681 acggcatctg caccgtcgtc ctgccccatc ggggcatttt gcacatcgcc cccaaaaacg
     4741 ccttgccgcc ttatgatgcc gccgccgaaa acgcgtggcg gctgcacgaa atccgcagcg
     4801 gttacccgtg gatatgcgcg gctaccaaag aaaccgccgt cgcccaaatg ctcaaccagc
     4861 acatcatcgg cggcgtacac ttcaaaaaag gctgctatcc gggacaggaa atcattgccc
     4921 gtgcccagta tcgggggcag gtcaaacgcg gcttggcggt attgtcggga aattcggccg
     4981 tcgaagcagg aaccctgctg acggcagacg gcgaagagac cggcatcgta ctcgacagcg
     5041 ttcaagattc ggaaaacttt accgcccttg ccgtgattaa attctccgcc gcgcaaaaag
     5101 aactgaccgc cccaaacggc agtatcttca aagcggttca tcccttcttt aaaaccgaaa
     5161 atgcagaata atccctctaa tgccgtctga acctttttca gacggcattt taaaaccgcc
     5221 cctgttttcg gttacaatgc cgcatcccca atttttcaaa ccaccgccat gtccaaaaaa
     5281 accaaacaag aactggaaaa caacaaactc agcaaacgcc tgcgccacgc cgtcggcgac
     5341 gcgattaacg atttcaacat gatcgagccg ggcgacaaaa tcatggtctg cctctccggc
     5401 ggaaaagaca gctatgccct cttggacatc ctgcgccagc tccaagccag cgcgccgatt
     5461 gatttccaac tggttgccgt caatctcgac caaaagcagc cgggcttccc cgaagaagta
     5521 ttaccgacct atctcgaaag catcggcgtt ccctacaaaa ttgttgaaga agacacctac
     5581 tccaccgtca aacgcgtatt agacgaaggc aaaacgactt gttcgctgtg cagccgcctg
     5641 cgccgcggca tcctctaccg cactgcaaaa gaattgggct gcaccaaaat cgccttggga
     5701 caccaccgcg acgacatcct cgccaccttg tttttaaata tgttttacgg cggcaaactc
     5761 aaagccatgc cgcccaagct ggtgagcgac aacggcgaac acatcgtcat ccgtccgctg
     5821 gcgtatgtaa aagaaaaaga cttaatcaaa tacgccgaat taaaacaatt ccccatcatc
     5881 ccgtgcaacc tctgcggctc gcaacccaac ctgcaacgcc aagtcatcgg cgatatgctg
     5941 cgcgattggg acaaacgctt ccccggccgc atcgaatcga tgttctccgc cctgcaaaac
     6001 gtcgtccctt cccaccttgc cgataccgaa ctcttcgact ttgtcggatt ggaacgcggt
     6061 caaaccctga aacacggcgg cgatttggcg tttgacagcg aaaaaatgcc cgaacgtttt
     6121 tcagacggca gcgaagaaga cgaaagcgaa atcaaaatcg cgccgcaaaa agccgaacgc
     6181 aaagtcatca acattctggc gaacaagccg aaaacctgcg gcgcgtaacc ccccgccgtc
     6241 aaaaatgccg tctgaagccc gtttggcgtt tcagacggta ttttaaaatc ccgaagtaga
     6301 cacttttcaa ccccaaacgc ctgctttttc cgtttcagct ttctgaaggt ttcccgttat
     6361 attactcggc aacaacgcgg cgaattttga cgggctgtac gggtatgttt tgataaaagc
     6421 cgcgcgtggc ggttttgacg cgggcgattt tggaaacggt gttcatgccg ctttcgaccc
     6481 tgccgaaaac ggtatagccg tattgtccgt ttttgtagtt gagtgaatca ttgtccgcca
     6541 gattgataaa gaattggctg gtggcggaat ccggatcggc cgtccgcgcc atggcgacgg
     6601 taccggcggt gtttttcaag ccgttgccgg attcgttggc aacggcctta tcgcttgcct
     6661 tttgtaccaa gtcttcggta aatccgccac cctggataac gaagccgtcg ataacgcggt
     6721 ggaaaatcgt attgtcgtaa aagccttttc gggcatagcg cacgaaatta gcaacggttt
     6781 tgggggcttt ggactcgtcc aaaaccaaac ggatattgcc catatcggtt tccatcaaaa
     6841 catgggttgc cgccatagac ggcagggaaa ccgccaaaag cagcgcggtt aaaacggttt
     6901 tgaatttggg tttcatcccg tcctcctaaa gccttcagac ggcattttca tttcatatgc
     6961 cgtctgaaag cgcgtgaacg ctattccaat gcgtctttga gcttttgttc gattaaatcc
     7021 gcatcaaacg atttggcaat caattcaaaa cgcgaatcgc gccgccatga gacttggttc
     7081 gcgccccact gtccgtctac ccagttgagc cacacccacg ttcccaatac ttgaaacacg
     7141 cctttggcgc ggacgagtcc ttcggtcatt ttgggcaggt cgttgaaaaa gttggtcagc
     7201 ttttcgccgt cgaaatcgcg tccggcgggg aatgtgaaac cttgcgactg gaagcccatg
     7261 gtgttgtccg gcagggcttt gaggcggtag cgtgattttt cgatgacggg aatatcgagc
     7321 caccggatgt cgagttgtgc gttttggact tcgaccactt tggctttggg cgggaacagt
     7381 tttgccgctt tgtcgtgaaa ttcggcaagc tgttcggtgg tgcataaatc ggttttgctg
     7441 gcgaccaata cgtcgcagat gccgatttgg tctttataca acgcctgttg cgcgtaatcg
     7501 gggttgatga actggcgcgg atcgacaacg gtaaagacgg caccgatttc caaaaggctg
     7561 tccagcggtt tggctttgag ttcgtcgatg acgctggctg cgtgcgccag tccgcttgcc
     7621 tcaatcatca ggcggtcggg cttggcatcg cgcagcattt tctgcacggt tacgcccatt
     7681 tgcgggccgg cggtgcaaca caaacagccg ccggcgattt ctgccacagg gatgccgttg
     7741 tcgctcaata ccgcgccgtc aatgccgatt tcgccgaact cgttgacgat gatgacccat
     7801 ttttcgttcg ggtctttttg cgccatcagg cttttgagcg cagtggtttt gcctgttccc
     7861 aaaaaacctg aaatcaggtg gactttggtt tttttcattt ctatgtgatg tcccacttta
     7921 aaatttgaag atagggtgtt ttaaatgatt aaataatgtt aaaagtgatg gcagctgtta
     7981 tcatgttctt catcaattga caattgttcc agcaaattcg atatatcggc tgacatcgcc
     8041 ggtatgacgt tcaaaaccgt ctcccaaacg acagacaggt tcatttcaaa gtagccatgg
     8101 gcaatcctat tgcgcaatac cccctcatcc ctatccaatt caaatatttg gtttcctcgg
     8161 caaattccgg atacgattta aggatatggg ttgcgttttc accgataagg aacaaattaa
     8221 aaacgactgc ctgcacagtc tttgtgtcgg cagaaaattg cccataatcc attttgtcgg
     8281 tatatagccg gatatattgc gctgcctgca atatctgttt caaatagacc gacaattctt
     8341 tctgcatttt cataatgcga cagcctcagt caacacctta tctctaaagt gggccgagat
     8401 gtcatcgggt gtcagcaaat cgacctttat gcccaagagt ttctgcaatt cctcttgcag
     8461 cccgcccaaa tccaataatg ttgtccctgt ttttgcatcc accaacaaat caatgtcgct
     8521 gttttccgta tcatctccgc gagaaaccga accgaatacc cttggattgc aaatcaatgg
     8581 atatttcccg aaaactgcca atatttcttt ctttctgctt tgcaacaaaa gagacggttt
     8641 cattatctgc tcctttcgaa aggcttatta tcaatgcaaa ccaccgatta ctgctgacat
     8701 tttttacaaa tcccggttaa aacaacgtgt tcttctttca gcgcaaagcc gctttcggca
     8761 acgcctgcgc gcagtgccgc ccactcgtgg ctgagggttt gctcgtccgc cgtgccgcat
     8821 tcggtgcaga ccaaaataaa cgcgctgtgg tgcgcttcgg cttcttcgtg gtcgtggcaa
     8881 tggtcgtcgc actcgtgctg cgcgtggctg cacaaaatat agccgttgac cgccgccact
     8941 ttgtgcaaaa cgccctgctc cgcccaaaaa tcaagggcgc ggtaggcggt aggcggtgca
     9001 agcacgccct cgctttgctg ctgcatctgc gacaagacgt tgtaggcttt aatcacgccg
     9061 ctttgctgca agacaatatc taatacctgc tcgcgcaaag cggttacctg caagccttcg
     9121 ctgcgtgcct gttcgataat tttctgtttg aaatttgttt tcataaattc tctgtttatg
     9181 ccgtctgaac aaccgatacg gcaggaggcg gttttatatt tgtattcaat tgctttattt
     9241 ggaaatcttt tccaacaatg cccgacagcc cgcatccgca agcctgaagg tttcttcaaa
     9301 atcgcccgta taccacggat cggggacatg gtcgtaaccg ctttcgggta tcaggtcggt
     9361 cagcttgaat attttttccg gccgcctgcc gaaggttctt tccaattcgg acaaattctt
     9421 gccgtccatc gcgatgatgc agtcaaacgc cgccgcatcg ctttggcgga ttttgcggct
     9481 ggtaaagcct gaagcatcga taccgtattt tttcaatatc tttgccgtct cgcggtgcat
     9541 atcttcgccg tcgtgccagc ccgatgtccc cgcgctgtcc gcttcaaggg gaatgcccgc
     9601 ttcggcggcg cggcggcgca aaatgtattc cgccatcggc gaacggcaga tgttgccgag
     9661 gcagacaaaa aggattttcg gttttttcat atcccctccc tgttccggcg cgatgccgtc
     9721 tgaagcggaa accctttcag acggcatcgg tcggttaatc gtaaccgcat aaggcagaac
     9781 cagccaaaac atccatctgg aagaaatggt ttggctaaat cttaggcata tttaataagt
     9841 gtccaatatt agaagccgta tgctccaaat agagactggc atttttcaaa ctatcttcta
     9901 aaggttcact tttctccaaa atagaaaagg cagcttggat attttcaaat ggcagggaag
     9961 gcaaatcttc aacgagactg ccacaaatag cgacaacagg aactccgaca ggggttcttt
    10021 ttgctacacc aataggcgct ttccctgcta aactttgacg atctagtctt ccttcaccaa
    10081 cgataaccaa gtcaacatct gacactttct tatcaaagtc aatcaagtcc aggcaggtgt
    10141 caattccaga tacgatactt gcctgagcaa aggcgcacaa accaccagcg atgcctccac
    10201 cagctcctgc tcctttaagt tttaatgttg caggggagac tttttcataa aaatcttgta
    10261 ttgcctgatc tacggcctca aacatagtag aatccaaccc tttttgcttg ccaaacgtat
    10321 aggtcgcacc ttggtgtcca cataagggac tcacaacatc tgctaaaata cgaatgtgaa
    10381 catcttcagg aatttcatag cgattttctg ttgaaacaga agctaggttt agtaaggatt
    10441 gaccgcaaac gggtaaggta tttccatcct catcataaaa ttgataacct aaaccagcag
    10501 caatcccaat acctccatca ttactggccg taccgccaac gccaatatag atttctttaa
    10561 tttcttgact aatgaggtgg cgaatcaatt ctccaatacc acgagtttgg atttgtaatg
    10621 gatttcgttt ctctagcggg atttttccaa gaccaaccaa atcagcaact tcgaataggg
    10681 ctagttgttc tttttgaaaa tagcgcatga cttctttttg tccaaaaggt cctgtcactt
    10741 gaagacattt ttcttctagg tcaagagaat gtcggatagc atctacagtg ccttctcccc
    10801 catcaccgac aggacagagg agacattcca catctgctat cgattgttgg aagcctcttt
    10861 ttattgcttc agctacctgt tgagctgtca agctttcctt aaacgaatcc ggtgcaatta
    10921 caatcttcat atttataatt catcctttcg tttcactcaa ggcacaacac agaatgaaaa
    10981 agtgttgtgc tctttatttt gatttattat ataaatgaga aagcctatca ctactacaaa
    11041 tcactatgcg ctgaaaaacg gattgtgccc ttcccgtttc aatgcttccg catagctcgg
    11101 gatgctttcc tgttcgccca agggattgtg caacaggtaa acgtgttcca cgcggcactc
    11161 cgccatcagg tcgaggaaat tttgctggat gacggcgatg ttgtcggtaa aggaaagctg
    11221 ccacgaaaac acctgcggca cgacttcaaa cgcgccgtcg gtcgcattaa acccgaataa
    11281 aacctgtccg cccgcctttg ccgcagcgac cacgccgacg cgcggactct gcatacggac
    11341 ggcgcgtatc gcgtttgccg caaacacatc catcgccata cgctggcgcg tgtggctgcc
    11401 gtctgaaagc gcgcgtaccg gagaatccgg ggaaagtgga ttggcaaaca gcgtaacgcc
    11461 ttccgatgca agattttcct gccatacctg cattaagggc aggcctgcct gaagaaggtt
    11521 ctgacccaat gccgtctgaa ccttgccgct gccgaaaccc agcgcgtaaa cgacgtggac
    11581 ggactgccct tcgggcaaca gcaaagggcg cggggcgaaa cggcgcaagt gttgcgccgc
    11641 cgcttcggta ttttgtttgg cacgccacca ctcatccggc gcgaccgcgc tcaaatcgga
    11701 agaatgcaca agataaggca gccattgcgt ctcttgcgtc aacgcgcgca ggtgcggata
    11761 gtttttcaaa caggcaaaca atgcctccgt cggcagcttc atctcgattg cccgttcttt
    11821 cttgcagccg gacaccagca caaccggcag ggcgaaccac tgcacttcgc cttctttctc
    11881 gcaatcgagc accgcgttca cactggaaag cagcgcggca taagttccgg catcgggcga
    11941 catcgtcaac gcgagggaaa ggttgatata gtggttttgc tcaagcatcc ccctgatttc
    12001 ggtttgaagc tgcccgaacg agagtttgcg tgaagcctgg gaagaattgt gcgccaactg
    12061 gtaggcattg agcagcaggt ggtttttaat ctgattttgg ggatacgggc gcgtatcggg
    12121 caaggtaaat gtctggttca tatgtgtcgt gtaccgtttt tcgcagtgtg tgtctttcct
    12181 gtttgaaaat atatcggacg gattgccgcc gggacttgcc cgtcaatccg ccgaaacgag
    12241 aaaatgcctg tctgccaagt ctgccaatat ttcttccaca tacacttcgg caggcggatg
    12301 gaatgtcaaa ccgtcgggcg tggtgctgac gatgttcaag ttttcagcaa ccgcatctat
    12361 caccgcctgt ttgctcgtcg gccgcgacaa ttgccgcata atgtacaaat gcccgtatcc
    12421 gaatgagggg gtgctttcgt tgtaacggtt ggcaggccgg acaaacgctt ccgcgccgtg
    12481 ttccacaaag gcggcggcat aatctgtaaa acgcgccggc acataggttt tatggtcttc
    12541 aaaaaatggt ttgcccgcat tgtctgatga aacagaaaaa cgcccgagaa tggtttgcaa
    12601 taaaagctgg gaattgattt tcatacggtc aaattccaga ccgggaaatc ggcgggcgag
    12661 gttttccgca acatcttccg ttttaaacgc ctctccggtt ttcatcacat cgcgtatgcc
    12721 cgaaagcagg gtatcatttt catcaaagtt gattgtttcc cctcttgccg ggcggaaatt
    12781 caaactttct atcacttcga cggcaaccga ctcatcgcgc ctgatattgt ccccgacttc
    12841 ctcacggcat aaaagcgaac ggcggaattg gcggtcggat aaaatatcgc tgtaaaattc
    12901 tttggcaata taatcgcccc ctgccaatgc cagaatccgc tcccgcgtat gctccgccat
    12961 ccaagaaaca aaagacgcgt gcaaatcggt atccccgata tatgcgagcc tgtggcgatt
    13021 agcccattcg atgaagccgt tgacgtaaat cgggtcgtta agcgcctcca tatattcgtg
    13081 tgcgatgtaa taaaaattat gattcaatat tttttgaatc gccggaagtt tgccgccgcc
    13141 gtccaagccc ttgtcgtttt ccaaaatttc cgccagcgcc ttgagcgcgt ccaagccttt
    13201 ccgcgtccgc gcttccaagg gttcttcaag cacatccctg ccggcaaagt acataatttc
    13261 gcgcaactgc tcctgccgtt tccagcccgg gtaaacattg tatgaaatat aggcaatgcc
    13321 gtgttcggtc aggttgttcc ggcaaatcga aaaaattttg tctttaactg cgtcaggcac
    13381 ccacgaccaa atgccgtgga cgatgatata gtcaaacttc ccgaatgact catcgatggt
    13441 caaaatatct ttttcttcca gacgcacatt tttcaagccc attttttcaa tgatggcgtt
    13501 gccctgcgca acctgcctgc cggacaagtc gataccgaca aattccgcat ccgggtaata
    13561 aagagcctgc gtgatgatat tgccgcccat cgaacagccc agctccaaga ccttggcttt
    13621 tgccgctgct gcaggcttca ggcccatcat gcgggcgcga gcctccaagg cgttgatggc
    13681 ggtttgggaa aatgctttgg attcatacat caaatcatca tatgaatttt tgatgttgga
    13741 cacgtccggc acaccgttct ccgttgcagc acgcgcaaag gcggcttttt tctttttgtt
    13801 cggcatattt tgtttgtctg aaggcactat tgcccgcaag tttaaccaat tcatcctacc
    13861 cgttcaacta aatcaaatgc catctgaagg cgcggagcgt acttcagact gcatctggga
    13921 ggcgcgaagg cttcagacgg catttttgcc gctttatttc aacgcggcat catagccgcc
    13981 gtcttcaacg gcttcaatca acgcccctgc atcggtttgc gcggggtcgt atccgacggt
    14041 cgcacttttg ttttcaaggc tgacttcgac gcttgccacg ccttttacgc cttccaatat
    14101 ccgggtaacg cttttgacgc agccgccgca gctcatgccg ccgatgtcga ggataagggt
    14161 ttccatcatt ttcctttcgt tggaactgca atctgacggg cgttattgta agtcggggcg
    14221 tgaacttggg caaacgcgga aacggtgcgg cggtttggaa aaagacggac gcttgcgcat
    14281 aatggcggca attcccatca ggacgacaac aatgaacgct tcgcaaaaac cctggttgag
    14341 catcatcgcc ttggcaatcg gcgcatttat agtggattaa caaaaaccag tacgtcgttg
    14401 cctcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccaag tgaaccggtt
    14461 ccgtactatt tgtactgtct gcggcttcgt tgccttgtcc tgatttttgt taatccacta
    14521 tacaacgtcg gtatcggcag cggcgcactg ttgggacatt gggtaacgca atatgtcggc
    14581 atttcccgta tcggcatcgc cggtatgctt gcggcggcgg ccgggctgtg gctgtgcctg
    14641 aacttgaacc gccatatcca aacctgaccg cgcgatgccg tctgaagccc ctcccgccct
    14701 tcagacggca ttttgattga agaaatatcc acccctgctt aaaataacgg gctttgccgt
    14761 gttttaacgc ctattttctt attccaagga tggtttatgc cgctgctgtc tgtcgagttc
    14821 gcactgttct ttctcgcttt cctgccgatt tactggggct tggcgaaata cccgtccgcc
    14881 caaaacctgc tgcttctggc tgccggtatg ggctggctct accatatcgg ccccgtattt
    14941 gcggcaatca tcgtccttta ttcctcctgc gtgtaccttt tgggcgaact gctccgttcc
    15001 gatcgcgaaa atacgcgccg cttttggctg gggtgcggca ttgccgcctc gctgaccgtc
    15061 ttgggctttt tcaaatattt cgactttttc cggccgctga ttgcccaata tgccggacaa
    15121 ggcggcgcaa tcgacatcct gatgccgctg gggctttcgt attacacctt ccagtcgctc
    15181 gcctatctgg tttactgctt ccgcgccccg cacgccgcgc gtttcgagtg gcacgagctg
    15241 ctgctgcacc tgagtttttt ccccaccgtt acctccggcc cgattatccg cgccgccgca
    15301 ttcaaaagcg cagacggcga gcaggcgggc gcattggcgc aaatccgtac ccgccaaccg
    15361 cgctcacccg tccgccccgc actcgccgtt tccctgattc tgctgggtat tgccaaaaaa
    15421 tggtggctgg cggggatgct ggcggaaaac tgggtgtcgc ccgtatttga aaatcccgcc
    15481 caattcgacg gctggggcgt attgggcggc gtgtacggct ataccttcca actcttttta
    15541 gacttttccg gatattccga tttggttatc ggcatggcga tgctgctggg tttccggctg
    15601 cccaaaaact tctccgcacc gcttcgtgct ttaaacatcc gcgcattttg ggacaaatgg
    15661 cacatcagcc tttccacctg gatacgcgac tacatctaca tccccttggg cggcagcaaa
    15721 aaaggctttt tacggacaca gctcaacctg atggcggcaa tggtactctc aggcatctgg
    15781 cacggctacg gctggaactt cctcatttgg ggcgcgctgc acggcacggc gctggtgctg
    15841 ctcaacacgg gcgatcgcta tttcggacgc aacgcgctat gccgtctgaa ataccttgcg
    15901 ccgctctcgt ggtttgttac cttccatttc gtctgcctta gctttgttgt cttcaatacc
    15961 gcaagccccg acgatgcagg cgcagttttc aatgccctct ttgccaatgc cagcggctgg
    16021 aatgcgccgc aacaggcaga catgctgctg cttgcctcgt ttgcagccgt gatgctgctc
    16081 tacccttacc tgcaacgcgc tttcgacggc gtggtcaaag gtttggaaaa aatcccgatg
    16141 tggctgtggt ttatccccgt ttccgccgtc ctgctgctga ttatcgtcct cgccccctcg
    16201 gggatacccg gctttattta tgccaatttt taagggtttg gacatgaaaa actttctttc
    16261 ccttttcgcc tccatattga tgtctgccct gattgccgtg tggttcagcc aaaaccccat
    16321 caacgcctac tggcagcaga cctaccaccg caacagcccg ctcgaaccgc tcgccgccta
    16381 cggatggtgg cggagcggtg tggcgttgca agaaaacgcc tacgcccttt cagacggcat
    16441 caaagccttc ctgtccggcg aaacgccgcc gacggctcaa gacggcggtt cggcagatat
    16501 gccgtctgaa gccgccgcat cagaagccgt ccctcaaacc ggtgaaacag aatggaaaca
    16561 agacaccgaa gccgccgccg tccgcagcgg cgacaaagtc ttttttgccg gcgactcgct
    16621 gatgcagggc gttgcaccct tcgtgcaaaa aagcctgaaa cagcaatacg gcatcgaatc
    16681 cgtcaacctc agcaaacaaa gcacggggct gtcctacccc tcattcttcg actggccgaa
    16741 aacgattgaa gaaaccctga aaaaacatcc cgaaatcagc gtgctcgccg tcttcctcgg
    16801 cccgaacgac ccgtgggatt tccccgtcgg caaacgatac ctcaaattcg cttccgacga
    16861 atgggcgcaa gaatacctga aacgcgtcga ccgcatcctt gaagccgcac acacgcaccg
    16921 cgtccaagtc gtctggctcg gcatccccta tatgaaaaaa gccaagctcg acgggcagat
    16981 gcgctacctc gacaaactgc tttcggaaca cctggaaggc aaaatcatcc tgattcccac
    17041 cgcgcacacc ctgagcggcg gggaagaccg ctacaccgac tccgtcaacg tcaacggcaa
    17101 acccgtccgc taccgcagca aggacggcat acactttacc gccgaaggac aaaaactgct
    17161 ggcggcaaaa ataatggaaa aaatcgtttt tgaaccaagt acgcaaccat caagtacaca
    17221 gccatgaacc ccaaacacct catcgcattt tccgccctgt tcgccgccac gcaggcagaa
    17281 gccctgcccg tcgcctccgt cagcctcgac accgttaccg tttccccgtc cgccccctac
    17341 accgatacaa acgggctctt gaccgactac ggcaacgccg ccgcctcgcc ttggatgaaa
    17401 aaaatccgat ccgtcgcaca aggcagcggc gagaccttcc gcatcctgca aatcggcgac
    17461 tcgcataccg ccggcgactt ctttaccgac agcctccgca aacgcctgca aaaaacttgg
    17521 ggcgacggcg gcataggctg ggtttacccc gccaacgtca aaggacagcg tatggcggcc
    17581 gtccgctata acggcaactg gcaaagcctc accagcagga acaacaccgg agacttcccg
    17641 ctcggcggca tcctcgccca caccggcagc ggcggcagca tgaccctgac cgcatcggac
    17701 ggcaagaccg gcaaacagcg catctccctg tttgccaaac ccctgctcgc cgaacaaacc
    17761 ctgaccgtca acggcaacac cgtctccgcc aacggcggcg gctggcaggt actggatacc
    17821 cgcgcggcac tgcccttgac catacagacc gaaatgccgt gggacatcgg cttcatcaac
    17881 atcgaaaatc ccgccggcgg cattaccgtt tccgccatgg gcatcaacgg cgcacaattg
    17941 acccattggt cgaaatggcg tgccgaccgt atgaacgacc ttgcccaaac cggcgccgat
    18001 ttggttatcc tttcctacgg caccaacgaa gccttcggcg acaacatcga cattgccgat
    18061 accgaacaga aatggctgga taccgtccgc caaatccgcg acagcctacc tgccgccggc
    18121 atcctcatca tcggcgcgcc cgaatccctg aaaaacacgc tcggcgtatg cggcacacgc
    18181 cccgtccgcc tgaccgaagt ccaacagatg cagcggcgca tcgcccggca gggacaaacc
    18241 atgttctggt cttggcaaaa cgccatgggc ggcatatgca gcatgaaaaa ctggctcaac
    18301 caaggatggg ccgccaaaga cggcgtacac ttctccgcca aaggctaccg gcgcgcggcg
    18361 gaaatgctcg ccgacagcct cgaagaactc gtccgcgccg ctgcaatcag gcaataatcg
    18421 gacaggaggc ggacggtatt tccgcaacag ggggatgccg tctgaaacgc ataccttcat
    18481 attgcttcag acggcatagc caccccgcgc acggtttgcc ggacgcaacc ggcattcgcc
    18541 tcaagcatcg gaaggacggg ggcgaacctc cggcatacgg cgcaaaggcg gcgtttgata
    18601 tgccgtctga aggcaaagat gataaactgc cgccttccgt tttcagacgg catattgttt
    18661 tcaaatgagg gcgttcgccg tccgcaacca taaaggaagt ttcatgaacc ggacttatgc
    18721 caatttctac ggaatgctcg ccgccgcctg ccgcaaaaac ggcaacggca cggcagtgtt
    18781 cgacggcaag gaaaaaaccg cctaccgcgc gctcaagcag gaagccgaag ccgttgcggc
    18841 gtatctgcaa aatatcggcg tgaagttcgg cgacacggtc gcgctggcgg tttccaattc
    18901 cacggaattt attaccgcct atttcgccgt atccgccatc ggcgcggttg ccgtaccgat
    18961 gaacacattt ttgaaaaaca gcgaatacgc gtatatcctg aacgactgca aggcgcgctt
    19021 cctgttcgcc tcggccggcc tgtcaaaaga attggcgggc ttgaaggcgc aaacgcccgt
    19081 cgaaaaaatc atttggacgg gccaaagccg tccggacggc gaaatggcgg aaggcgatgc
    19141 cttttttgaa gacgtgcgcc gcttccccga aaaacccgac ttgggccgcc aaccccggat
    19201 aaatgatttg gcacacatca tctacacctc cggcacgacg gggcatccca aaggtgcgct
    19261 aatcagttac gccaacctgt tcgccaacct gaacggcatc gaacgcatct ttaaaatttc
    19321 caaacgcgac cgctttatcg ttttcctgcc gatgttccac agcttcacgc tgacggctat
    19381 ggtgctgctg ccgatttata tggcgtgttc gattattttg gtcaaatccg ttttcccgtt
    19441 ttccaatgtt ttgaaacaga ccctgctcaa acgcgcgacc gtgtttttgg gcgtacccgc
    19501 gatttacacc gcgatgagca aggcgaaaat cccttggtat ttcagatggt tcaaccgcat
    19561 ccgcctgttt atcagcggcg gcgcgccttt ggcggaacaa accatcctcg atttcaaagc
    19621 caagttcccc cgcgccaaat tgctggaagg ctacggactg agcgaagcct ctcccgtcgt
    19681 cgccgtcaat acgcccgaga ggcaaaaagc ccgcagcgtc ggcatccccc tgcccggttt
    19741 ggaagccaaa gccgtcgatg aagaattggt cgaagtgccg cgcggcgaag tgggcgaact
    19801 gatcgtcagg ggcggttcgg tgatgcgggg ctacctcaat atgcctgccg ccaccgatga
    19861 aaccatcgtc aacggctggt tgaaaacggg cgatttcgtt accatagacg aagacggctt
    19921 tatctttatc gtcgaccgca aaaaagattt gattatttcc aaaggtcaaa acgtctatcc
    19981 gcgcgaaatc gaagaagaaa tctacaaact cgatgccgtc gaagccgccg ccgtcatcgg
    20041 cgtgaaagac cgttatgccg acgaggaaat cgtcgccttc gtccaattga aggaaggtat
    20101 ggatttgggc gagaacgaaa tccgccgcca cctgcgtacc gtgctggcaa atttcaaaat
    20161 ccccaaacaa atccacttta aagacgggct gccgcgcaac gctacgggca aggtattgaa
    20221 acgggtgttg aaggagcagt ttgacggaaa caaatgaacg ccgtgccgtc cgaagccccg
    20281 tccggcaaaa aaaatgcggt gaatctgctt caccgcattt ttttggcaaa cgccggagcg
    20341 aggatattta ctctgccaat tccacctgct cgtgcgccat cagttcctga ccgacatcat
    20401 ccaacaacat caaataacgt tcgtagtttt tcaaaatgtc ggcaatcagc tcgtccttgt
    20461 tcatatactg cacatcgtac ccgacgcgcc cgtcgaaaaa ataagcgtag ggtttgtaag
    20521 ttgtctgatg ccggatatgc ggcagcttgc cgtcgttaat caactggtcg gatacatcct
    20581 gcccgacaga cttaatcccg tacataaaat cgcgcatcgt ctctttccga atgacgaact
    20641 cgattgcggg ctcgtcctga tgaaacatct tatcgacccg gacgctcaag ccgtattctt
    20701 ccgaaagctc ccgttgcaac tcgtgcatag cgggcgatgc ggtatgtttg aggaatttta
    20761 aaatatcctg ctcctgcgtc tggctcatta tctgcaccag ccgttctttc cacttgccgc
    20821 ccgtccaaaa tacactggta gggttaaccc gggtctcaaa atatttctta tccgcactca
    20881 agcctttcca caggctgaaa cacattatca gcatcagcag ggcaaacggc agggaaacaa
    20941 tcagggtcat agactgcagg ttgccgagtc cgcccgagcg catcagcaaa acggcaacgg
    21001 cagacatcag cacgccccac ataaccgcct gccaccgtgg cgcgctcaag cctttgtccc
    21061 gagaggtaat attgttcagg acataaatcc cggaatcggc agaagttaca aaaaacagag
    21121 aaatgaccag caggctgacg atgctcgtca attcgggcag ggggaggtaa ttaaagaatt
    21181 taaaaagcag cgtttccgga gaggaggtca tcttttcgag cattcccccc gcaaccccgt
    21241 cattcagcca aatcgccgta ttgccgaaga cggtaaacca caaaacgccg aacaggccgg
    21301 ggatgagcaa aaccccgaag acaaactcgc ggatggtgcg cccctttgaa atgcgcgcga
    21361 taaacaaacc cacaaacggc gcccaagaac accaccacgc ccaataaagc accgtccaag
    21421 attcaaacca cggcttgtgt tcccgttcgt acgcataagt tttaaaactg aggcgcacca
    21481 gatttccgag gtagttccct atgttgtcgc cgaatgccga caacaggtaa acagtgggac
    21541 ccgccgccaa aacaaaaaac agcagcaaaa acgcaaggct caggttcaac tcgctcaaca
    21601 ccttcacgcc cttccccacg ccggatattg ccgaaacgac ggcgagggac atgacggcgg
    21661 cgataatcaa aacctgcacg ctgaagctgt tttcggcaat ccagcccatt tcctgcaatc
    21721 cggcgcccag ttgcgaagcc ccgaacccca atgtggtgat gatgccgaaa aaagtagcaa
    21781 gcaacgccat aatatcaatg gcatcgccga accttccgga aattttttct ttcaacaggg
    21841 gataaaaaca agaacgcagg gcaagcggca gcttgtagcg gaaaccgaaa taagccaaag
    21901 ccaatgcaat cgtaccgtac accgaccaag cgtgaacgcc ccaatggaac accgtgtgca
    21961 gcaatgcctg ctgctgcctg tgttccggcg tgccggccgt aatgtccgaa aaataatgca
    22021 tcaacggctc tgccacgccg aaaaacatca gacccacgcc catcccggcc gcaaacagca
    22081 tcgccagcca cgacaggaag ccgaattccg gcacatcttc atcccgtccg agcctgatgt
    22141 ttcccaaact gctgaccgag agtatcagca ggaaacccag aaaaatggaa aacgttaaaa
    22201 cataaaacca gccgaactcg gtaaaaatga cttcttttgc ccgatcgagc cacatctgca
    22261 cctgatccgg cacggttaaa accaatacca ccaaaacaca cacaaaaaac aaagtcgtca
    22321 aaataaccat cggattaaat gacgttcggc gttctataaa ttcagacagg gacaaacctt
    22381 ctcactcctt tgttaaaaac agacaaaccc ggtcatcggg caaagcggtc aaaacctgcc
    22441 gacaaaatac cggcttccgg atgacgaaat gcacaaaccg gccgaaattg ttataatcga
    22501 aaaaattaaa aatcaatacg gattattcta tcagattgat ttatcgatat ttattatatc
    22561 attaatatag tttgatttca aaccggcgga aaatgcgccg ccttccgcag gcgcgccctc
    22621 cccgccatcg gggcgtttaa cgccggattg ccgatgcggt acaatggctg atatgaagaa
    22681 aattacccct caaaacctgc gccccctgct ttcggaaagc ttgggacata ccgattttgt
    22741 caacgtcctc aacgcactga ttaaattttt gcgccgtggc ggcaaaaaat gtgcggggga
    22801 gcgtttcgac ctgattatcg acacattcaa acaagatagg gaattgctgt cccgcttcag
    22861 ccggtgtttt tacatctggc tcgcgcaaat acacatttat ccggcactca tcaaactcgg
    22921 catcttctcg cgccacagct ttgcccgaga aatgggcata cgcatctacg aacgcttcag
    22981 cccgtcatat aaagattttg ccaacttggg cgaagtcttc ctttatcttt tccattccga
    23041 aaacgacgac aaatggctgc aaacgctcaa tatccgccaa tggctggttt tatacgaact
    23101 catccggagc cacgccgagc cgtccaaatt gcagacggcg ggcatccgcc ttgccgatgc
    23161 gcgtttgcgc gccatcgaaa tgctgtctgt ctggacggca tccgaagcca tcgaacccga
    23221 cctcatccgc atcgccccgc gcctgctgga agccgattct tccttcgtcg ccctccaacg
    23281 cgaaaccgcc aaactggtcg aacactaccg caacggcacc acgccttacg acaccgccca
    23341 cctcgaagtg atgttcgacc aatgtttcag ccagattgac tatttgcgcc gcaaagggac
    23401 gggcgccggc tccggttcgt cggtcaaagt cgcccacctg ctcgaacggc tccggcagac
    23461 cgtagaccgt ctaaagctgc tcaccgacat ccaaaccggt gccggcaaca gcaaccgcct
    23521 gaccatcgcg ctgatgaact ccctcatcta cgcggcggtc gaacaataca gcacccgcca
    23581 cctgcgccgc agcagcatcc gtatgctcgc ccgcagcatt accgaaaaca aaagccacca
    23641 cggcgaacac tacatcaccc gcaaccgcaa agaatatttc aaaatgttct actcggcggc
    23701 aggcggcggc atcatcatcg ccctgatggc gctgctcaaa atccgcatcg gcacactcgg
    23761 cctcagcccc ttcctgactt ccttgtcggc tgggttcaac tacggcatcg gctttatgat
    23821 catccatatg ctgcactgca ccgtcgccac caaacagccc gcgatgactg ccgccagctt
    23881 tgccgaacag gtcgacctca acgaaggcgg caaagcggtg gacaacaaac tcgccaagct
    23941 cctcatcgac gtatgccgct cccaaagtgt cgccgtcttc ggcaacgttt ccatcgccat
    24001 ccttttggcg tgcgccatat cgttcggcta tgcccatctg caccaacagc ccatactcga
    24061 tgcccacacc gccgcctacc agttcaaatc catagacatc atcgcttacc cgacgctgtg
    24121 gtatgccgcc attgcaggtc tgtggctgtt ctgctccggc atcatcgcag gcttcttcga
    24181 caaccgcgcc gactacctca acctgcgcca acgcctgccc ttcaacccct tgctgcgtaa
    24241 aataatgcgc cccggacccc gccgcgtcct cgccgcctac atccacaaac actacggctc
    24301 actggcaggc aacttcatct tcgggatgct cttgggtatg accggctatt tcggacacct
    24361 cctcgggctg ccgctggaca tccgccacgt cgccttttcc tccgccaacc tcggctatgc
    24421 cgccgtcagc ggcaacgtcg gtttgggcac gttcgtactc ggcattttca gcgtcctcgc
    24481 catcggcctg gtcaacctct gcgtcagctt cagcctcgcc ctcttcgtcg ccctgcgctc
    24541 gcgcggcacg aaaatcggca gcatccgcaa tctgattaag agtttttgga atcagattaa
    24601 aagcaatccc tgcatacttt tcctcccgcc cgccaaagaa cagggacatc ctccttcgga
    24661 caagccttga ccggcaatgc cgtctgaagc gggattcgcc ccgaataccg ccctgatgcg
    24721 ggaaatcccc ataaa
//
LOCUS       DAXHSJ010000010        23910 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000010 DAXHSJ010000000
VERSION     DAXHSJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.010"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(66..1967)
                     /gene="thiC"
                     /locus_tag="ACFM8U_000304"
     CDS             complement(66..1967)
                     /gene="thiC"
                     /locus_tag="ACFM8U_000304"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC6333610.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKHGLPHIRTAWLDE
                     RGDTEILPKLSSEYGTERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
     regulatory      complement(2058..2157)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2307..2383
                     /locus_tag="ACFM8U_000305"
     tRNA            2307..2383
                     /locus_tag="ACFM8U_000305"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2341..2343,aa:Met,seq:cat)
     gene            2572..3447
                     /locus_tag="ACFM8U_000306"
     CDS             2572..3447
                     /locus_tag="ACFM8U_000306"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6333611.1"
                     /translation="MPTVHFTESVSKQDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSPEWAERIKKDWEAGCSESSDGIFLNADGWPDMGRRLQHLARGWHCAGLLDGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVAAMLSENMMHDAQLVTLDAFCRYGLI
                     DVAHPLSEWLDGIRL"
     gene            3456..4394
                     /locus_tag="ACFM8U_000307"
     CDS             3456..4394
                     /locus_tag="ACFM8U_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6333612.1"
                     /translation="MLELNGLCKRFGGKTVADDICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIIRPDGGEIRLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRNGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQNAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(4837..6612)
                     /gene="ptsP"
                     /locus_tag="ACFM8U_000308"
     CDS             complement(4837..6612)
                     /gene="ptsP"
                     /locus_tag="ACFM8U_000308"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC6333613.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     IVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQINSVSVEEEADFKGRK"
     gene            complement(6612..6881)
                     /locus_tag="ACFM8U_000309"
     CDS             complement(6612..6881)
                     /locus_tag="ACFM8U_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC6333614.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFQSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(6950..7387)
                     /locus_tag="ACFM8U_000310"
     CDS             complement(6950..7387)
                     /locus_tag="ACFM8U_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC6333615.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(7468..8031)
                     /locus_tag="ACFM8U_000311"
     CDS             complement(7468..8031)
                     /locus_tag="ACFM8U_000311"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC6333616.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(8100..8924)
                     /locus_tag="ACFM8U_000312"
     CDS             complement(8100..8924)
                     /locus_tag="ACFM8U_000312"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC6333617.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKDQEIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            9015..9647
                     /locus_tag="ACFM8U_000313"
     CDS             9015..9647
                     /locus_tag="ACFM8U_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC6333618.1"
                     /translation="MTLRYEILSVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(9853..11637)
                     /gene="rmuC"
                     /locus_tag="ACFM8U_000314"
     CDS             complement(9853..11637)
                     /gene="rmuC"
                     /locus_tag="ACFM8U_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC6333619.1"
                     /translation="MELMTVLLLLAALLSGVLFTWLLMKSRFQGVLAVLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYEHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRVETDLEESRQTLRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHIDLQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYVAEDEAV"
     gene            complement(11976..12764)
                     /locus_tag="ACFM8U_000315"
     CDS             complement(11976..12764)
                     /locus_tag="ACFM8U_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC6333620.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(12779..14128)
                     /locus_tag="ACFM8U_000316"
     CDS             complement(12779..14128)
                     /locus_tag="ACFM8U_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC6333621.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(14147..14728)
                     /gene="petA"
                     /locus_tag="ACFM8U_000317"
     CDS             complement(14147..14728)
                     /gene="petA"
                     /locus_tag="ACFM8U_000317"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC6333622.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(14851..15600)
                     /locus_tag="ACFM8U_000318"
     CDS             complement(14851..15600)
                     /locus_tag="ACFM8U_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC6333623.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            15731..16660
                     /locus_tag="ACFM8U_000319"
     CDS             15731..16660
                     /locus_tag="ACFM8U_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6333624.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHDLLPQVTDAERDLARIIEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPIWPQIELDIVSGFQADPIGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAKDHPLAAKTVWEAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(16916..17308)
                     /gene="rpsI"
                     /locus_tag="ACFM8U_000320"
     CDS             complement(16916..17308)
                     /gene="rpsI"
                     /locus_tag="ACFM8U_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC6333625.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(17318..17749)
                     /gene="rplM"
                     /locus_tag="ACFM8U_000321"
     CDS             complement(17318..17749)
                     /gene="rplM"
                     /locus_tag="ACFM8U_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC6333626.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(17927..18106)
                     /gene="ssrS"
                     /locus_tag="ACFM8U_000322"
     ncRNA           complement(17927..18106)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFM8U_000322"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(18112..18417)
                     /locus_tag="ACFM8U_000323"
     CDS             complement(18112..18417)
                     /locus_tag="ACFM8U_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC6333627.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(18428..18757)
                     /locus_tag="ACFM8U_000324"
     CDS             complement(18428..18757)
                     /locus_tag="ACFM8U_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333628.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(18812..19801)
                     /locus_tag="ACFM8U_000325"
     CDS             complement(18812..19801)
                     /locus_tag="ACFM8U_000325"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC6333629.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMKEERE
                     NKHGLPGFPFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(19924..22626)
                     /gene="ppc"
                     /locus_tag="ACFM8U_000326"
     CDS             complement(19924..22626)
                     /gene="ppc"
                     /locus_tag="ACFM8U_000326"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC6333630.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESCGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKIIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            22669..23562
                     /locus_tag="ACFM8U_000327"
     CDS             22669..23562
                     /locus_tag="ACFM8U_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6333631.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKLKTEALADRLKRINHTVNVRAVNEKLDGCRL
                     TGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAER"
BASE COUNT         5114 a         6458 c         6436 g         5902 t
ORIGIN      
        1 caagcgaaag aatgccgtct gaagcctttt ttccgggttt cagacggcat tttttgcttg
       61 acgtttcaac tgtaaatctt cgcgcctttt ttgacgaact cgaccgcttt ttcctccatt
      121 ccctgccgtt gggctttttg cttgtcggcg tagtcgcgca cttcctgcgt gattttcatc
      181 gagcagaatt tggggccgca catcgagcag aagtgggcga ttttcgcgcc ttcggcaggc
      241 agggtttcgt cgtggaagct ctcggcacgt tcggggtcga ggcttaagcg gaattggtcg
      301 cgccagcgga attcgaaacg cgccttgctc agggcgttgt cgcgtaattg cgcacccggc
      361 cagcctttgg cgaggtcggc ggcgtgagcg gcgagtttgt aggtaatgat gccggtgcgc
      421 acgtcttctt tgtcgggcag gccgagatgc tctttcgggg tcacgtagca gagcatggcg
      481 gtgccgtacc agccgatatt ggccgcgcct atgcccgagg tgatgtggtc gtagccgggt
      541 gcgatgtcgg taacgagcgg accgagcgtg taaaaaggtg cttcaaagca gtgttgcagc
      601 tcttcggtca tgttttcttt gacgcgttgc agcggtacat gcccggggcc ttcgatcatg
      661 acttgtacgt cgtgtttcca cgctttatcg gtcaattcgc ccaaggtgtg caattcggcg
      721 aactgggatt cgtcgttggc atcggcaatg cagccggggc gcaggccgtc gccgaggctg
      781 aatgatacgt catacgcttt cataatttcg cagatttcgt cgaaatgcgt gtagaggaaa
      841 ttttcccgat gatgtgccaa acaccatttc gccataatcg aaccgccgcg cgagacgatg
      901 ccggtgaggc ggttggcggt catcggcaca taacgcagca acacgcccgc gtgtatggtg
      961 aaatagtcca cgccttgctc cgcctgttcg atgagggtgt cgcggaacaa atcccaagtc
     1021 aaatcttcgg cgatgccgcc ggttttttcc aacgcttggt aaatcggcac ggtgccgatg
     1081 gggacgggcg cgttgcggat aatccattcg cgtgtttcgt ggatgtgcgc gccggtggac
     1141 aaatccataa tcgtgtccgc accccaacgc agcgaccaca ccattttttc gacttcttcg
     1201 gtcaggctgg aggttacggc ggagttgccc aagttgccgt tgattttgac gcggaagttg
     1261 cggccgataa tcatcggttc gagttcgggg tggttgatgt tggcgggaat aatcgcgcgt
     1321 ccggcggcga tttctcggcg cacgaattca ggcgtgattt ggtcgggatg ggtcgggata
     1381 ttcgcgccga aactttgccc tgcgtgttgt ttcaagagct tggcgtattc cggtttttgg
     1441 gacaattcgt ctaattttaa acgttcgcgt atggcgacaa actccatttc gggcgtgata
     1501 atgccttggc gcgcgtagtg aagctgggtt acgttgctgc cgcttttcgc gcggcgcggg
     1561 cgggtgattt ggttgaagcg cagatgggcg gttttcggat cgtgtgcgcg ttcggtgccg
     1621 tattcgctgg agagcttggg caggatttcg gtatcgccgc gttcgtccag ccacgccgtg
     1681 cggatgtgcg gcaaaccgtg tttcaggtcg atgtgcgccg ccggatcgcc gtacacgccg
     1741 ctggtgtcat agacgggaat cggcgggttg gcttccgtgc cttgcgccgt gtaggtgtcg
     1801 tcctgacgga tttcgcgcaa aggcacgcgg atgtcgtcgc ggctgccttg cagatacacg
     1861 cgctccgagt tcggatattt aaagcggatg ccgatgtctt cgctcaagtc ggcaagctcg
     1921 cgcgcttcgt tgccggaagt tttggcggtt ttttttggcg tagtcataaa aaatgctcct
     1981 gttttctcgt ttagaataaa gaaacaggag cgttttgcgt tttcagacgg catttgaaaa
     2041 ccaatgccgt ctgaaaagca gaatccgtga aaactcccca cgcaggtatt atcccgatcg
     2101 ggtgtaaagg gtatttctca gccgcctaaa catcaggcag cacccctgtt tcaatgttaa
     2161 ccaaaattaa atcacgaaca tgaacttttg taaagaaaat aatatttcaa atcaggcata
     2221 aaccgccgga cggcaaaatt ttatgatttt tcgcggaaat aatgtttgac aacataaaaa
     2281 atctgccgta tagtttcatc ttctgacgcg ggatggagca gcatggtagc tcgtcgggct
     2341 cataacccga aggtcgtagg ttcgaatcct gctcccgcaa ccaaatatca aacccctcgg
     2401 ttcaatgccg aggggttttg ttttgcctgt ttcctgtttc ctgccgcctc cgttttttgc
     2461 cggattttcc ctcctgccgc aatatcggaa cggcagcccg ccgtctgttt gcggttgcaa
     2521 attcaggcgg tttggctaca atcttccgca ttgtgttcaa gaaagccgac tatgccgacc
     2581 gtccatttta ccgaatccgt cagcaaacaa gaccttgatg ccctgttcga gtgggcaaaa
     2641 gcaagttacg gtgcagaaag ttgctggaaa acgctgtatc tgaacggtct gcctttgggt
     2701 aacctgtcgc cggaatgggc ggagcgcatc aaaaaagact gggaggcagg ctgctcggag
     2761 tcttcagacg gcattttcct gaatgcggac ggctggccag atatgggcag acgcttgcag
     2821 cacctcgctc gcggttggca ctgtgcggga ctgttggacg gctggcgcaa cgagtgtttc
     2881 gacctgaccg acggcggcgg aaaccccttg ttcacgctcg aacgcgccgc tttccgtccg
     2941 ttcggactgc tcagccgcgc cgtccatctc aacggactga ccgaatcgga cggccgatgg
     3001 catttctgga taggcaagcg cagtccgcac aaagcagtcg atcccgacaa actcgacaat
     3061 attgccgccg gcggtgtttc cggcggcgaa atgccgtctg aagccgtgtg ccgcgaaagc
     3121 agcgaagaag ccggtttaga taaaacgctg tttccgctca tccgcccggt atcgcggctg
     3181 cacagcctgc gccccgtcag ccggggtgtg cacaatgaaa tcctgtatgt gttcgatgcc
     3241 gtcctgcccg aaaccttcct gcctgaaaat caggatggcg aggttgcgtg ttttgagaaa
     3301 atggacatcg gcagcctggt tgccgccatg ttgtcggaaa acatgatgca cgacgcgcaa
     3361 ctggttacgc tggacgcgtt ttgccgttac ggtctgattg atgtcgccca tccgctgtcc
     3421 gagtggctgg acggcatacg tttataggat gcgccatgct tgaactgaac ggactctgca
     3481 aacgcttcgg cggcaaaacg gttgccgacg atatctgcct gactgtcggg cgcggcaaaa
     3541 tactcgccgt tttggggcgg tcgggctgcg gaaaatcaac actgctgaat atgattgcgg
     3601 ggattatccg tccggacggc ggcgaaattc ggctgaacgg ggaaaacatt acccgtatgc
     3661 cgcccgaaaa acgccgtatt tcgctgatgt ttcaagatta cgcgctgttt ccccatatga
     3721 gtgcgctgga aaatgcggca ttcggtttga aaatgcaaaa aatgccgaaa gccgaagccg
     3781 aacgccttgc catggcggca cttgccgaag tcggactgga aaatgaggcg caccgcaagc
     3841 ctgaaaaact ttccggaggc gagaagcaac ggttggcact ggcgcgcgct ttggttgtcc
     3901 gcccttcctt gctgttgctg gacgaatcgt tttccagttt ggacacgcat ttgcgcgacc
     3961 ggctgcgccg catgactgcc gaacgtatcc gaaacggcgg catccctgcc gttttggtaa
     4021 cgcattcgcc cgaagaggcc tgcacggcgg cggacgaaat cgccgtcatg cacgagggga
     4081 aaatccttca atgcggtacg cccgaaacct tggttcaaac gcctgccggc gtgcaggtcg
     4141 cccgactgat gggtttgccc aataccgacg atgaccgcca tattccgcaa aatgcggtgc
     4201 gtttcgacca agacggcatg gagtgccgcg tattatcccg tacctgtttg cccgaatcgt
     4261 tcagcctgtc cgtcctccat ccggaacacg gcatcctgtg gctgaacctc gatatgccgc
     4321 acgccggtga aatatcggga aacgatacgg tacgcatcca tatcgaagac agggaaatcg
     4381 tccgcttccg ctgatgcttc ttaaaaacaa aatgccgtct gaaaaccttt cagacggcat
     4441 tttttaccaa agcagccata ttttttatca gggctgcaaa atttatccga aacaacagaa
     4501 accaccctgc cgtcattccc gcaaaagcgg gaatctagaa cccaacacgg caaaaattta
     4561 tccgaagcga caacaatctt ttcatcgtca ttcccgcgca ggcgggaatc cagaacgtaa
     4621 aatctaaaga aaccgtttta cccgacaagt ttccgtgccg acagacctag attcccgcct
     4681 gcgcgggaat gacggatttt aggtttctga ttttggtttt ctgtttttga gggaatggcc
     4741 gattttgggt ttctgtttcg gttttctatt ttgcaagaat ggcaaaattt cagattgcgg
     4801 gcattgttaa gtatttctat ttttcacctg ccgtatttat ttccgccctt tgaagtcggc
     4861 ttcttcctcg acagacacgc tgttgatctg tttgatcagc ttttccgact tctcttcgtc
     4921 ttcgcagcgg atgactttca caatatcgct ttcgagctgc ccgacattgc tgtgcagaat
     4981 gatgtttttg acgggcagga tgttgttggg gttcatggaa aaacggcgca gccccatacc
     5041 caataaaacg cgggtaaacg cggtatcgcc cgccatctcg ccgcatacgg atacgtcttt
     5101 gtccatgcgg ttggcggtac ggatgacgtg ttgcagcatt ttcagcacgg cgggatggcc
     5161 gggctggtag aggtggctga cgctgtcgtc gccgcgatcg acggacaaga tgtattgaat
     5221 caggtcgttg gtaccgacgg agatgaaatc gaccagtttc aaaatactgc cgacggtcag
     5281 cgcggcagac ggaatttcaa tcatacagcc gatgccgact ttaccgaagg catcgccgcg
     5341 ttcggcaagc tggcgttgcg cggtgtcgag gtggatgagg cactggcgca cttcggatac
     5401 ggaggtaatc atcggccaca tcatgcggac gggaccgtgt accgccgcac ggaggatggc
     5461 gcgcatctgg gtgcggaaca tgaccggttc ggcaaggcac aggcggatgc cggtcatgcc
     5521 cagcgcgggg ttgaggctgc cgttgggcgt gctgtttttc ccgaaccagc gcgggttttt
     5581 gtccacaccc aaatcgactg tccgtatcgt tacgcttttg cccttcattt ttttgacaat
     5641 cgcgctgtac acttcgtact gctcgtcttc agacggcatc gtatcgcggt tcaggtaaag
     5701 aaactcgctg cggaacagcc cgatgccgtc tgcgccgaga ttgtgcagcg gtttcacgtc
     5761 ttcggcggat tctatattgc ccacaagctc gatgcagacc ccgtcagcgg tggcggcggc
     5821 ggtttttttg agcttgttca aatcgcgttt gtggctgcgg tattcgcggg cacggcggcg
     5881 gtattcgttc aacaccgact catccggcgc gataatcaac acgccgttga taccgtccac
     5941 aatgaccgtt tcgccctcgg taatcagttt gcgcgcgttg tgcagcccga cgacggacgg
     6001 gatgtccaag ctcctgccca aaatcgccgt atgcccggtg gggccgccgg catcggtaac
     6061 gaaggcggca atgcgctgct ctttaaacaa aaccgtgtcg gcgggcgaaa ggtcgtttgc
     6121 aatcagcacg atttcgtcaa acaggttgtc ggcaacttcc aactcgttgc cctgcccgat
     6181 caggttgttg tggatgcggc ggacgacttg cagcatatcc tgtttgcgtt cgcgcaaata
     6241 ggcatcgtcc atattgtcga attgggcggc gagtttgtcg ctctgctgct tcaatgccca
     6301 ctcggcgttg attttttgtt cccttaaaat atcgacgggt tcgcgcgaca aggtaacatc
     6361 ggttaagagc atcaggtgta gcgagatgaa cgcgcccaac tcggtcgggg cgttttcggg
     6421 aatcgcgctg cggagctgtt ccaactcttt gcgcgtggct ttgacggcgg catcgaaacg
     6481 ttcggcttcg gcatcggtgt ccgcctccgc aacatcatac tgcggcactt cctccgtacc
     6541 gcgcgcaatc aggtgggcgc aaccgacggc aatgcctttg cccgccgcca cgccgtgcag
     6601 cacgatactc attattcgcc ctcgccgaag tagccgttga ttaagtcggt cagggcgcgc
     6661 atcgcttccg cctcgtccgc gccgtccgtc tccagttcga tgaccgtacc cttggcggcg
     6721 gcgagcatca tcagccccat aatgcttttg ccgttgacgc ggctgtcgtt tttcgtaacc
     6781 cagacttcgc tttgaaactg agacgcggtt tgggtgaatt tgctggaggc acgggcgtga
     6841 agtccgagtt tgttgatgat ttcgatggat tgtttgagca tttcgattcc cgtgttatgt
     6901 atatcggcag cagacgccgt ttaaaatgtt ttcctgccct gccgcttctt cagacggcat
     6961 cgccgctgcg ccggcacacc aaatcttcgg gcgcggacgt gatggcgaaa atgcctttta
     7021 ccgccgcctc cctgacgcat tcggtaaagg cggcaaggtc ttccgccgcc ggcgaatatt
     7081 ggacggcctt aaccatcatc ggcgcgttca gcccggtcaa aatcgccgat ttgttttcgc
     7141 gcacgaggcg gcgggcggca ttgcaggggg tcgcgccgaa aatatcggtc ataatcagca
     7201 cgccgtcgtt gtcgggaaac tcctgaagcg cggcaatggc gttgttgatg atgtcgtctt
     7261 ggtcttccgt cggttgcacg ccgagtatgc ggacgttttc aggcagtccg cccggaaaaa
     7321 aatgatgtgc cagcttgcgg taggcttcgc ctatggtttc gtgtgtgatg attaaaagcc
     7381 ctatcatatt atgcgtcctg ttcctcgttg tcctaccggc gtatgggcgc gatgccgtct
     7441 gaaaggcctt cagacggcat cgcgtcctta ttttccgtcc aatgcgtaaa tctcgcccag
     7501 attgcgccag cagcccgccg catccatgcc gtaaccgaaa acataacggt tcggcacatc
     7561 cagtccgaca taatcggctc ggataggctt gggtttgtca atcagcttgt tggcgaacac
     7621 cgccgcacgg cagcttgccg cacccatttc caaaagtttg gcttgaatgg cggacatcgt
     7681 atgcccttcg tccaaaatat cgtccagcac gacgacgtgc ctgccccgga tttgttccgc
     7741 atcgggcata cgcttccagt tgaacgcgcc gccctccagc ttgtcgccgt aacgggaaac
     7801 gtgaacataa tcaaaatcta agggaaaacg caacagcggc agcaactgcc ccgtaaacac
     7861 caccgcgccg cccatcacgg gcagcagcag cggatatttg ccgcccaaat cgcgcgtaat
     7921 ctcgtccgcc actttttgca gtgcggcacg gcattggcct tggtcgaaca aaagatcagc
     7981 gttttcaagc atcgcctgtg tttcaaggcg tttggtttct aaatcggtca tatgtcggaa
     8041 tcggtcggta aaaggaaaat tataaaccaa agtatcggat gccgtctgaa ctgtcctgct
     8101 cagcggtcgg tacgcacgcg cacaaatgtg gcaaatttcg gcgtgccttt ccgcgtaaag
     8161 ccacggtaac ggtaggtaat cagcgtgccg attttgggcg ggttgtcgcg gtctttgtcc
     8221 ttgaaaccgc tgccgatgcg gaattcgccg tgtcggtttt tgcagccgac cgcgcccagc
     8281 cgtccggcgt ttcgcccttt gccctcatag tgccgcgtta ccgtgcattc gtcgtcgtat
     8341 tggcttttca gcttcaataa ttggctgctc ctgccgccgc tataacggga ttcgggctgg
     8401 cgcagcatca cgccttcgcc gccctgcgct tcgatttgtt ttaaaaagtc catcgcgtgc
     8461 tgccggtcgc gcactttgat ttgcgggatg atggtaatcg gcgcgttcgg atgcgttttc
     8521 agccactgcg ttgcgactgc caaacgttgg tagagattgc cctgcgcctt gggtacgtcg
     8581 aaaacgtgca gacggatgcc gcgccagtct gaagaaacag aacgcacggt agcggaaatc
     8641 tgctcgaact gaccgcgtcc gctatacaat tcgccgtcca aaggataagg cggaaactgc
     8701 gcggtaaagc ctttgggggg cgtaaacgcg tagccctgac ggcttatcag gtgctttccg
     8761 tcccaatagg cgcgcacgcc gtcgagtttc tcgctcattg cccagcctgc aatctcttgg
     8821 tctttgtatt cctgcgccag catcaaatcc gccgcgcctg ctgatgcagg gatgaaaacc
     8881 gccgtaaaaa tcggtatgat gccgccgatt gtcttcttaa tcatctgatt cccccaatat
     8941 caaaacaggc ggcaaaccgc cataaaacaa acggcaaacc cgatgccgtc tgaaaaaccg
     9001 tttaggaaca cgccatgacc ctacgttacg aaatcctctc cgttaccccc ttccgccaaa
     9061 actgcaccct gatttgggac gacgaaagcg gcgaagccgt cctgaccgat gtcggcggcg
     9121 acgtgccgtt cctgctgcaa gcgttggcaa accgcaaact tacgctcacg gcaatctggc
     9181 tgacgcacgg ccatctcgat cacgcgggcg gcgtggtcga aatgttgaaa acgcataaag
     9241 tccctgtcct cgggccgcat ccggacgatg aattcctgct ccaatcgctg ccgcaaacca
     9301 ccgcgcaata cggatttccc gtctcgcccg cctttgcgcc gaaccgttgg ctcgaagaag
     9361 gcgaaacgct cacggtcgga cgctatgcct ttcaagtgct gcatattccg ggccatacgc
     9421 cgggacatat cgtcttttat tgtgccgagg cggaattgct gattgcgggc gacgtgctgt
     9481 tttacgaaac cataggcaga accgattttc cgcgcggcaa ccacgccgac ttaatcaata
     9541 atatccgcaa caaattattc acccttcccg aaaccgtgca agttgtcgcc ggacacgggc
     9601 gtatgacttc catcggacac gaaaaacggc acaatccgtt tttctaaccg acttcactac
     9661 ggtcttcaga cggcgtcatc tgcactgatg ccgtctgaaa cgcaaaaggc tcaggcaacc
     9721 gccgccttgc cggtgtacct cgccggacaa ggctttggta agtacttcaa accaacccca
     9781 aatcagaacc ggtacagtta cgccgcgctt tcggcattcc cgcccctgct gaaacaagct
     9841 tttccgcaca agtcagactg cttcatcttc tgccacatat tccaaagatt ccgacaacgc
     9901 cgttgtttca ttggcacgtt cggtcaaatc gcgtgcaagc tgcttgtttg ccttcacacc
     9961 caacagacgc agtttctcgg cgcggccgac cagattcccg cgcccctcgg caagttgctt
    10021 aaatgccgtc tgaaagctgt tttgcgcctg atcgatgcct ttgccgacgc tttcgagcgt
    10081 ctgtacaaag ccgacaaatt tgtcgtacag cttgccgcct tcgtccgcaa tcgccagcgc
    10141 gttttggttt tgctgctcgt tgcgccaaat attcgccacc gtccgcaaag tcgccagcag
    10201 cgtactgggg ccgaccagca taatccgttt gtcgaaacac tcttggaaca agccagcgtc
    10261 attctgcaac gccaacaggt aggccggttc gacagggata aacataaaga cgaaatccaa
    10321 tgtgttcaca ccttccaaat cggtgtaatc cttcagcgac aagcctttca tgtgtgcacg
    10381 gatgctggca acgtgtgccg ccagttcgcg tgccgccgta tccgcatccg ccgcctgcgt
    10441 gtagcgcaca taagctgtca gcgagacctt ggaatcaatc acaatctgct tgttgtcggg
    10501 caggttgacc aaaacgtcgg gctggaggcg gcgcgtgccg ccgtcttcct cttttcggac
    10561 ggatgccgcc tgaaccacat attcgcgccc tttctggagg ccggaatttt ccaaaaccgt
    10621 ttccagaatc atttcgcccc aattgccctg aaccttgttc tgcgtaccgg tcagcgcgtt
    10681 ggtcagtgcc tttgcctcgc tgtgcagctg cgcattcaat ccttgaagcc gtttcaattc
    10741 gttttccaac gtcagccgct cgcgcgattc tttatcatag gtttgcttga ccaactcgcc
    10801 gaaaccgtgg atgcgttcgt tcaacgggtt caaaacctga tggagctgct ctcggttctg
    10861 ctcggtaaaa cggcggcttt tttcttccaa aatcgtgttg gcaagatttt gaaactgatc
    10921 gctcaaactc ttgcgcgcct cgcccagtaa ggacagcttc tcttcagaag caaggcgttc
    10981 ctgttcgatt tgcgttgcca aacgttcgtt ttcaaccgcc aaaccctgtg ccttttcctg
    11041 caaatcgata tgcgactgcc tcaaccgctc cgcttctgcc tctttttcct gcaaatgggc
    11101 aatctgtttt tcggctgcgg caaaacggtt gcccacatcg gaaaggtcgt tttgcacgtc
    11161 gcggagagtt tggcggcttt cttccaaatc ggtttcgact ctttggcgga tttggcgttc
    11221 cagcgcatat tgatcggcaa acgccttccg ctcctgtccg agctgggttg ccaaacgttc
    11281 gttttcaacc gccaaaccct gcgccttttc ctgcaactcg atatacgact gcttcagacg
    11341 cgccgactcc gcctcttttt cctgcaaatg ggcaatctgc ttttcggctg cggaaaaacg
    11401 gttgcccaaa gcataatttt cgtcttgcaa atgctcgtat ttcccatcca aaacggctaa
    11461 ttccgatgca atttctgcgt gtgcctgttc gacaaaatca catcttgccg ccttttccgc
    11521 caattgcgcg ttcaatacgg ctaatacacc ttgaaaccgg ctcttcatca gtaaccatgt
    11581 aaacaacacg cctgacagca aagccgccag caatagcaac acagtcatca gctccataaa
    11641 tcatcctaat attcaaacat tttcacaccg gacaaaaccg ccgcttattc cgattctacc
    11701 tgtttgttcg acatagctcc aaaaaatata gcggattggc tttaaacctg ttcgacatct
    11761 ccttaccatg ctgcttgcgg tttcagacct tttcctaatt caatatcaat ctgccacaaa
    11821 ccctgattaa gttcccgatg tctgacattt ttagaatgat gccgtctgaa atgttgcagc
    11881 tatgttcaga cggcatacgg attcaggctt ttcaaacggc aggcaaaatg aaaaaagggc
    11941 aaaccctaaa ggatttgccc ttttgttcca aacgcttagt gtacgtcttt ccaatattct
    12001 tttttcagga aatacgccaa aggcagcata accgcaaata ggaaaatcat cacgacatag
    12061 cctatacgtt tgcgttgcag ttgtgcaggt tcgcccatgt acacaaggta attgaccaaa
    12121 tcgcgtacat atgcgtcgta ctctttttgg atcactttgc cgttaggcag acggcggctg
    12181 tgcaaaccgg tagattccca atacagctta ggcttcatct cgccgtgttc gtcttttacc
    12241 ataaccggct gacctttggc atccaactca acggcttgaa cgccttgctg ttcccacaac
    12301 gggtggggca taccgacttt atcgaataca gtattgttcc agccgctcgg acgggtcgga
    12361 tctttataga agccgcgcat ataggcgtaa aggtagtctg cacctttgga acgcgcaatc
    12421 aacgtcaaat cgggcggagc agcaccaaac cattttgccg catctttcgg gttcatcgcc
    12481 gaatgcatga catcgccgac attatcggtg gtaaacatca ggtttttctt gatttcttcg
    12541 tcagtcaaac cgatgtcttt caaacggttg aaacgcatac cgcttgccga gtggcaggac
    12601 aggcaatagt tggtaaagat ttgtgcgccg cgctgcaggc tgacttggtc gcgcaaatcg
    12661 atatcgactt tctcatagtg tgcatgaccg cccgaagcaa atgccgcacc catgggtact
    12721 gccaacagca aggcagcaaa ccaatttttc atagcttgtt tcattttgac tgccctcatc
    12781 agatgttggt tgcaaacagg taagcaccca cggcggtaat gccgacgtaa acaaagaaca
    12841 tgattttttg tttggcggtg ctcatggtta cgcgttcggg aaccggtttg tttttatcca
    12901 gcttggtata gaacggcata cccaggaaga atgcgaaata gacgaaagac aggatgcgcg
    12961 cgaccaaagt acgcgtatcg gttgccacca tcgcgcccaa aatacccaaa ccgataaagg
    13021 aaatgatgaa caagaccaat gcggttttga aaatcggacc gcggtaacgg acggatttca
    13081 cctcgcccct atccagccaa ggcagcaggg caatcaacac gaccgccgcg cccataccga
    13141 ttacacccca aacctgcgtt cccaagaacg agggaatcgc acgcaaaatg gcgtagaacg
    13201 gtgtgaaata ccataccggc gcaatgtgcg gaggtgtttt cagcgcattt gccgcatcga
    13261 agttcggcgc ttcgaggaag tagccgccgc cttccggtgc gaagaacaat acggcacaga
    13321 agacaatcag gaatacaacc acacccaaga tgtctttaac ggtatagtaa ggatggaatg
    13381 ggataccgtc gcgcgggata ccgttttcgt ctttgttttt cttgatttca acgccgtcag
    13441 ggttgttaga acccacttcg tgcagggcga tgatgtgcgc cacaaccaag ccgagcaata
    13501 ccagaggtac cgcgataacg tgcagggcaa agaaacggtt cagggtaacg tcggaaacgt
    13561 tgaagtcgcc gcggatccaa gtggacaaat ccggaccgat aacagggatg gcggagaaca
    13621 ggttaataat tacctgcgca ccccaaaagg acatctgacc ccaaggcagc aggtagccca
    13681 taaaggcttc cgccatcaat gccaagaaaa tcagggagcc gaaaatccac accaattcac
    13741 ggggtttttt gtacgaaccg taaatcaaac cacggaacat gtgcagataa acaacaatga
    13801 agaagaaaga tgcgccggtg gaatgcatgt agcggataat ccaaccgccg gacacgtcgc
    13861 gcatgatgta ctcgactgcg gtaaaggcag caggcagatg gtaggcgtta aggttgccgt
    13921 ccggtttgta gttcatggtc aggaaaatgc cgctgacgat ttgaatcacg aggacaagca
    13981 gggccaaaga gccgaagtag taccaaaaat taaagttttt cggtgcgtag tattgagcca
    14041 aatgctcatt ccacatttta gacaggggga aacgagcgtc catccagcct aacaatgctt
    14101 ttgctttgct attggtttgg tttgccataa ttatcgttcc ttattcttag tcttcgccca
    14161 ccaagatagt tgtgtcgctc aagtatttat atggcgggac aaccaggttg gtcggggcag
    14221 gaacaccttt atatacgcgg ccggccaagt cgaatttcga accgtggcag gggcagaaga
    14281 agccgccttt ccagtctgca cccaaatcgg cgggggcaat gtcgggacgg aaggtgggcg
    14341 agcagcccaa atgggtgcag ataccgatgg cgacaaggat gttcggctta atcgaacggg
    14401 tctcgttttt agcatactcc ggctgctgtt ccgcatcgga attgggatcg gtaagttcgc
    14461 cgttcaggcc tttcaggtct ttaagctgct gatctgtacg gttgagcacc caaatcggtt
    14521 tgccttgcca ctcggcggtc agcagctgac ccgcttcgat tttactgaca tccacctcga
    14581 cggcagcacc ggcggccttg gctttttccg aagggaaaaa actggccaca aacggcgttg
    14641 ccacacccaa tgctgccact ccgcccgcgc cgcaggtcgc gagtgtcagg aaacggcggc
    14701 ggccattgtt gatttcttga ttatccatta ttcagtcgtc ctaatatttt gggaataccg
    14761 agccattaaa cgttgcaatt ttacccagtt tgcagtgata ctcaaagcat tatttaaaat
    14821 aaggtaaagt tttatgatat ttctcaagac tcaagccgga ttgttttcgt caaaatggca
    14881 cacttccaac ccgaaaacct ctgccgccga ttctgccagc gcgcgtacgc cgtaacgttc
    14941 cgtcgcgtga tgccctgccg aaatgaaagc cgtacccgtt tcattggcaa ggtggtattg
    15001 ggcttcggaa atttcccccg tcaaatacag atcgacacct tcgtctattg ccgtctgaaa
    15061 aaacccctgc gccccgccgc tgcaccatgc aacccgtcgg atttcgcgtt cgggtttgcc
    15121 gatgacgaca ggcttacgtt gcaaaactgt ttcaatatgc gccgccaatg cgccgagtgt
    15181 cttggcttgt ttcaggctgc ccgagttgag caggttttgt tcgccgaacc gtttttctgt
    15241 cgcaaaaccc aatctgtcgg cgagttgggc attgttgccc agtgtgggat gtgcatccag
    15301 gggcagatgg tagcctgcca tattgatgtc gtgccgtaac agtgcggcaa tccgttcttt
    15361 tttccaacca gtaacggtcg gcaactcgtt tttccagaac ataccgtgat gtaccaaaag
    15421 caaatctgcc ttctgctcca cagcaaaatc aatcgctgcc ctgcttgccg ttaccgacgt
    15481 aacgattttc ccgatatatt ccctcccttc aacctgcaaa ccgttagggg cgtaatcttt
    15541 aaacaacgct gtctgcaatg tttcattaca ccaagtcaga aaatccctgc gcaataccat
    15601 cttttttcct aatcgcttta aacaagcggg cattctaatc gcaaaatgtc tggaattcac
    15661 atttttccga tttgcacccg catatgaatt attttaatat gcgccggttc aatatgccgt
    15721 ctgaaacctc atggattcca tcatcgaact gcgccacctc aagaccctgc tggcacttga
    15781 agaaaccggc agcgtctccc ttgccgccaa acgggttttc cttacccaat ctgcactttc
    15841 ccaccaaatc cgtatgcttg aaaaccacta cggcgcaccg ttgttcgaac gaaaatccac
    15901 gcccttgcgc tttaccccgg ttggagaaag gctgttacgc ctcgctcacg acctgttgcc
    15961 tcaagttacc gatgcagaac gtgatttggc acgcattata gaaggagagg caggagaatt
    16021 gcggattgcc gtcgaatgcc atacctgttt cgactggctc atgcccgcaa tgggcgaatt
    16081 ccgcccgata tggccccaaa tcgaattgga tatcgtatcg ggattccaag cggaccctat
    16141 cggactgctg ctgcaacacc gcgccgatct tgccattgtt tccgaagcgg aaaaacaaaa
    16201 cggtatttct ttccaaccgc tgtttgccta cgaaatggtc ggcatctgcg ccaaagatca
    16261 cccgcttgcc gccaaaactg tctgggaagc cgaagacttt atcggggaaa ccctgattac
    16321 ttatcccgtt cccgacgaga tgctggattt gcccaaaaaa atcctgattc cgaaaaacat
    16381 caacccgccg cgccgacaca gcgaactgac catcgccatt atccaactgg ttgccagcag
    16441 acgtggcatt gccgcccttc cctattggac agtcatgccc taccttgaaa aaggctatgt
    16501 cgttcaccgg cagattactg ccgacggatt gcaaagcaaa ctgtatgccg ccatccgtac
    16561 cgaagatacg gacaagagct atctgaacaa tttttgccaa atcatacgcg aacgcggttt
    16621 tgcagatttg cccggactga gcgaactgga accggtctga ccccttattc aaccataccc
    16681 ggcagttttt ctattttttc atgtatagtg gattaacaaa aaccagtaca gcgttgcctc
    16741 gccttgccat actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
    16801 ccactatact gtttttgatt tttgcccaat ctgtaatctt tagattgcca atgggaaacc
    16861 gtctactaca aataaaaaaa ccctgcgata agcagggttt tttgaatttc caacattaac
    16921 gtttggagaa ttgttttgca cggcgtgctt tgcgcagacc cggtttttta cgttcgactt
    16981 cgcgggcatc gcgggtaaca aaaccggctt gagacaaggc gggtttcaac gcggcatcga
    17041 agtcgatcag ggcacgggta atgccgtggc ggattgcgcc ggactggccg gtttcgccgc
    17101 cgccaacaac attgactttg atgtcgaaag attcggcgtt ttcagtcaga accaagggtt
    17161 ggcgaacaac cattcggctg gtttcgcgtg cgaagaattc gtcaacggga cggccgttta
    17221 cgatgatttg acctgtacct ttaatcagga atacacgagc cactgaactt ttgcggcggc
    17281 ctgtgccgta gtagtattta ccgttcatgt cgcgtcctta tttcagttcc aaaactttgg
    17341 gttgttgcgc agcatgggcg tgttccgcac ccgcatacac tttcagtttt ttaatcatgg
    17401 cgtaacccaa aggacctttg ggcagcatac cttttacagc ttgttccaaa gcgcggcccg
    17461 ggaattgctc ttgcatttcg cggaaagtgc gttcgtagat accgcctggg aaaccggaat
    17521 ggcggaagta ttttttatct tcgaatttgg caccggttac acgcagtttg tccgcattga
    17581 taacaatgat gtaatcgccg gtatcgacgt ggggggtgta ttcaggtttg tgtttgccac
    17641 gcagacggct ggcgacttcg gccgcaacgc gacccaagac tttgtcttgg gcatcgatga
    17701 cgaaccattc gcgcttcacc tcgtggggtt tcgctgaaaa ggttttcata gtggaaatcc
    17761 aaatagatat agaaagttgt aaattttaaa gacaggattc gattttgtca atcgcattac
    17821 cgcgttacgg aaggattttc cggatttcgg cagactgcat actgcttttt cgggcggggc
    17881 ggcggccaat gtgaaaaacc gcatcgttgc gatgcggttt tgaatgggaa tccccgcgag
    17941 agccgtttcg gccgaatccg cttgaacctt gctgacaagg cggctgcctc gggtagtttc
    18001 gggtgcgtcc gcaaaaggac gctcgcgccc actactgctc ccggcaacct taagcgaact
    18061 tattggttca aaggaatata tgccttcgcg gacaccgcag ggaaaaaggg gttattcctg
    18121 cgccaagcgg gatagtgctt tttggcaggc gttgtccata tcggctattt tacgcgcaaa
    18181 atcgccgatt gccaaatcgc cgccgttcag ggaggttttc aacaggtcgt ggacgacgtt
    18241 gagcgcggcc ataatgacga ttttttcgct gtccgcgacg cgtccgcctt cgcggatggc
    18301 ttcggctttg ccgttgagca ttccgactgc ctgcaacagt gtgtcttttt cttctgccgg
    18361 cgtgttgacg gtcagccggg cgtgcatgac ttcgatgtgg acttgttcga tgttcatcct
    18421 ttaatcctta ttgctgcgtt tcctgccatt gggggaggcg cgctgccagt gcgctgattt
    18481 tttccctgct ctgttcgagc aggctgcggt atcgtgtatt ttcttctgtc aggctgtcga
    18541 ttttgttttg caggtcttct ttgagtttgc cgacttggac gagcagggct tcgctgagtt
    18601 cgtcgacggc ggtttcgtgt tcgagttttt gccgctcgtg cgcccgtttg agttcggcga
    18661 cggtttcttt gaggcggcgg ttttcgctga cgagggtttc gaatttttgt accagcgtat
    18721 aaacgctgct ttcgagtttt tcgatatttt gtttcataac cttacctgtc cgtatgccgt
    18781 ctgaaggctt cagacggcat ctgtctgttg tttattcaaa acgcgcgctg cgttccatca
    18841 gcctttcgac gacctgttgc ggggtcattt ctttgcggat gagttgcagc agggtttggg
    18901 taatcggcat gtcgatttgg tacttacagg cagtattgaa gacttcttct atcgtgctga
    18961 ccccttcgga aacgtgtccg atttcgacca gcacctgatg cagttccttg ccttctgcca
    19021 aacccaagcc gacgcggcgg ttgcgtgaga gtgcgccggt gcaggtcaag atgaggtcgc
    19081 cgatgcctgc cagccccatc atggttttgg gctgtgcgcc cattgcggag gcaaggcggg
    19141 tgatttcagc taatccgcgc gtaaccagtg cggcgcgggc gttaagcccg tactctagac
    19201 cgtcggacaa tccggtggca atcgccataa cattttttac cgcgccgccg actgccacgc
    19261 cgataacatc ggtactgccg taaagcctca tgacggtcgt gttgagctgc ggtacgagtt
    19321 cttcaatcca ctcttggttt tcggaggcga ggacgacggc gcagggcagt tgtttggcga
    19381 gttcctgtgc aaaactcggg ccggagagta cgccgatttt cttattgtcg ggcaatactt
    19441 ctttcaatac ttgaaaggtc agcagccctg tatcctgctc gaatcctttg caggcggcga
    19501 ggacggggag gtgtcccgcg ccgtactgtt tgagcagctc tgcgctgctt ctcaatccgg
    19561 caacggaggt tacgataagg acaagtccgc tgtctttgag cgcgtctgcc aaatccgcac
    19621 acacttccag cgtttcagga aaggggaaac cgggcagccc gtgcttgttt tcacgttctt
    19681 ccttcatttg gcggacttga tctgcgttgc gcgtccatag ggatacgcgg ttgccgtgtt
    19741 gggaaaaatg cagggcgagc gccgtacccc acgaacctgc gccgataacg gtaattttca
    19801 ttggttgtct ttcaacatat cactgccgtt cactttaaaa caatcgctgt ttctctgcaa
    19861 gtgcagacag ggaaatgccg tctgaaaggc gttcagacgg cattttgccc cgatgcggca
    19921 ctatcagcct gtattgcgca aaccttgcgc cacgccgttg atggtcaggt gaaccatcag
    19981 cagcgcgtgc ggattgtcgg gttctttacg caggcgtttg agcatggcga cctgcaggcc
    20041 gttgagcgcg ttcaggtatg gaatcctcaa ggcgagcgaa cgggcgaggc tgcggttgtc
    20101 gcgcaaaagc tcttcggttt gcagcaggtc gagcagtgct ttgcggctgc gtcggtattc
    20161 ttccttaatc atcccgaaga tgatttttgc cttatcgggc gattcgctca agccggcata
    20221 gttttccgcg agggtgatgt cggttttcgc catcacttgt tccatattgg agagcatggc
    20281 ttggaagaac gggttgcttt gggcatgttc gcgcagggcg gcgagggttt cgggtttgtc
    20341 ttcgcacaag gtttctaccg cgctgccgaa accgtaccaa gccggcagca tgaggcggtt
    20401 ttgcatccag gagaataccc acggaatcgc gcgcaagtcc tgaatccgcg ccaaggtttt
    20461 gcggctggcg ggacggctgc ctaggttgag ggtggcgatt tcctgaatcg ggctggtttg
    20521 cagaaagtag tcgatgaagt cgggatgggt aatcagttcg cggtagtatt tgaacgatac
    20581 gtccgacaat gcctgcatca gtttggcatc agggtctttt ttatccggca ggatgctggc
    20641 ttccaaagtc gcggcaacca aggtttccaa gttgcgttgg gcattgccgg ggtcggcgta
    20701 tttggcggta atgacttcgc cttgttcggt aatgcggatt tgtcccgcca cgctgcccgc
    20761 cggttgggcg agaatggctt ggtaagaagg gccgccgccg cgtccgacgc tgccgccgcg
    20821 accgtggaac aggcgcatgc ggacatcgta ttttttgaag agttcgacca agcccaattc
    20881 cgcctgatac agacaccatg agctggtaac atagccgccg tctttattgg agtcggagta
    20941 gccgagcata atttcttgga tgttgccgca gctttcgagc agggcatcgt accaatcgag
    21001 gcggaacatg gtttccatga ccggacaggc gttttcgagt gcttcgatgg tttcaaacag
    21061 cggcacgata ttgatgcggc tgcgcggttt gccgttttcc accgccagca ggccgctttc
    21121 tttcagcagc aatgccaagg cgagcaggtc gctgggttgt tcgcagttgg aaataatgct
    21181 ttgtgtaacg gcatcttcgc cgaattcgtc tttgattttg cgcgcttcgt tgaaaattgc
    21241 cagttcgtgg cgggtatggt cgctgtatgt gatgaacggg ctgtacagcg gacgttgatg
    21301 gctcaattcg cgcaacaggg cggcttgttt ttgctcttcg ttcaggctgt tgtagtcttc
    21361 caagcctgcg tgttggaaaa gctcggcaac cacatcggcg tgtttgcctg cgtgttggcg
    21421 caggtcgagc ggcatcatgt ggaagccgaa cacggacacg gaacggatga ggtctgccaa
    21481 acggccttca gcaagcaggc ggctgccgtt gtcgataagg gaacgttgca attttttcaa
    21541 atcatcgaga aattcttgtg ccgaagcata cggctcgaga aagccgaatt tgcagcccat
    21601 gcccaaaccg agcgcgcgcg ctttgcccat agcgcgcgcc atgatgtaag caatggcgcg
    21661 gcggtagggt tcttcggtgc gagcgatttc ttcgtcaggc gagagggctg ccagtgccat
    21721 tacgtcgtcg ttgactttga cgcggcggat ggagagcggc agttcgcggt aaagtttgtc
    21781 gagttcgcca cggtaaaaac ggaacacggc atcggcgtgg cggcggaagg caaaacgcaa
    21841 ggtttcggca gaaacgaacg gattgccgtc gcggtcgcca ccgatccagc cgccgatttt
    21901 gaggatgttc ggaacgcaga catcgggata gaccatctga aagtcgtgtt ccatattgcg
    21961 gtagagcttg ggcagggctt cgaaaaagct catcgggaag atggacacgc cgttgttgat
    22021 ttcgtcgttg acgctgagtt tgtggcggcg cgtttcgctg gtctgccaca agcccagcag
    22081 gatagtgtcg atttcgcggc gcagccgtgc cagcgcgtcg gcattggtgc agcgttcgcg
    22141 ttgcggcaac agtgcgcgga tgcggcggtt gaagcttaag acggtttggc gttgcacttc
    22201 ggtcgggtgc gcggtcaaaa cggcggtaac ggacgtattg tccaactgcc gctgcaccga
    22261 tttgccgtcg gctttccccg ctttgagcct gcggacggtt tccgtcaggc tgccttccgc
    22321 gccgccgcgt ccggcttctt cgtggatttg gcggcggcgt tcgtggtgca cgtcttcggc
    22381 gatattcaaa atctgggcga acaggccgca ggccaaggtt aaatcgtggg tttgttgttc
    22441 gtccaattgc ggcaatactt tttcaatcaa tgccgcgctg tcgtcggaag tggacaagag
    22501 tttgaccgtt tcgacaacca acggcgaggc ttcttcgtgc aggaggttga acagggattg
    22561 tttcagaaat tccgcgtccg ccgccaaagc cgcgtccttc ggattgttca gaatatgcag
    22621 ttgcatgatt tttctctcat tgccgtaaat actgtaaatg tacctcaaat gccgcatccg
    22681 tgccaaaccg ttcacacttt aaccgcccgt gtcccgaaat gccgtctgaa gttgaacgcc
    22741 gcccgacggc agcgttacaa tcgcccgcaa ctgttttttc cgaacatcat catgaccacg
    22801 accgaacacg acaacgacga cgaactcctg ctgcggtaca gccgccacat cctcttggac
    22861 gaaatcggca tcgaagggca gcagaaactt tccgccgcgc atattttggt cgtcggctgc
    22921 ggcggtttgg gtgccgccgc cctgccctac cttgccgctt ccggcatcgg cacgctgacc
    22981 atagccgatt ccgacacggt cgagctgcac aacctgcaac gccaagtcgc atttgacgag
    23041 ggcgatgtcg gcaaactcaa aaccgaagcc ttggcagacc gcctgaaacg catcaaccat
    23101 accgtcaacg tccgcgccgt caacgaaaaa ctcgacggct gccgcctgac cggtttggtt
    23161 caagccgccg acatcgtttt agactgctgc gacaactacg ccacgcggca agccgtcaac
    23221 cgtgcctgcg tacaaacgaa aacaccgctg gtttcagggg cggcggtacg ctttgaaggg
    23281 caacttgccg tgtaccgtcc cgacttgccc gactcgccgt gttacgcctg cctgtttgac
    23341 ggcggatcgg cttcagacgg catctgttcc ctcttcggcg tattctcgcc gctggtcggc
    23401 atcatcggca gtacccaagc ggcggaggct ctgaaaatcc tgctggatgc gggcgaaccg
    23461 tcgcacggca ggctggcggt ttaccgtgcc ttggaagggg gctggcaata tttcgacctg
    23521 ccgcgcaacc ccgaatgccc ggtttgcggc gcggaacggt aaaaccctgc cgccgtttca
    23581 gacggcatcc aaacggatgc ggaggaacgg ttttaaaaat ttaaaaattt acatttcttt
    23641 gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaataaac ttaccttata attgcagttg
    23701 ttttagcaat gtctgtttcg cagactcatt gagtaagacg ttttccccgt aatgtgtttg
    23761 gccgtctgtc ccctttgggt tcggacggct tttttttggc tgtgtatgaa tacccggttg
    23821 gttttatctg tttacagggg aaaaccgctt atttccgttc gggcggaaaa cggttccatc
    23881 ggataaaagg cattttgtcc gactgattaa
//
LOCUS       DAXHSJ010000011        23655 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000011 DAXHSJ010000000
VERSION     DAXHSJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23655
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.011"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..808
                     /locus_tag="ACFM8U_000328"
     CDS             <1..808
                     /locus_tag="ACFM8U_000328"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6333632.1"
                     /translation="RYRTPNIKYIYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINC
                     QVLLDFLSKDDIDMPDVVFLNAVKYDKGSVSHFGEDYQPEKILNQSKVEVLKGLCRFR
                     KFPGSACNKIIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQG
                     RKDSKTGTVSEKSIKSLLYILEKNAEMEFDRDISSYLYSFLSYEYLVLLFIMTSKNIE
                     CDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            849..1379
                     /locus_tag="ACFM8U_000329"
     CDS             849..1379
                     /locus_tag="ACFM8U_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC6333633.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1396..1527)
                     /locus_tag="ACFM8U_000330"
     CDS             complement(1396..1527)
                     /locus_tag="ACFM8U_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HFC6333634.1"
                     /translation="MPSKPEKRASDGIFACNSNADGQNQGASDTVIGHLVRVNLGLT"
     gene            complement(1546..3141)
                     /locus_tag="ACFM8U_000331"
     CDS             complement(1546..3141)
                     /locus_tag="ACFM8U_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC6333635.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3248..3643)
                     /gene="hisI"
                     /locus_tag="ACFM8U_000332"
     CDS             complement(3248..3643)
                     /gene="hisI"
                     /locus_tag="ACFM8U_000332"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC6333636.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3674..4441)
                     /gene="hisF"
                     /locus_tag="ACFM8U_000333"
     CDS             complement(3674..4441)
                     /gene="hisF"
                     /locus_tag="ACFM8U_000333"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC6333637.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPGNTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4454..5191)
                     /gene="hisA"
                     /locus_tag="ACFM8U_000334"
     CDS             complement(4454..5191)
                     /gene="hisA"
                     /locus_tag="ACFM8U_000334"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC6333638.1"
                     /translation="MLLIPAIDLKEGRCVRLRQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5224..5862)
                     /gene="hisH"
                     /locus_tag="ACFM8U_000335"
     CDS             complement(5224..5862)
                     /gene="hisH"
                     /locus_tag="ACFM8U_000335"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC6333639.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRDGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5993..7495)
                     /gene="pta"
                     /locus_tag="ACFM8U_000336"
     CDS             complement(5993..7495)
                     /gene="pta"
                     /locus_tag="ACFM8U_000336"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC6333640.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDIVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7680..8738)
                     /gene="fbpC"
                     /locus_tag="ACFM8U_000337"
     CDS             complement(7680..8738)
                     /gene="fbpC"
                     /locus_tag="ACFM8U_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC6333641.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8759..10276)
                     /gene="fbpB"
                     /locus_tag="ACFM8U_000338"
     CDS             complement(8759..10276)
                     /gene="fbpB"
                     /locus_tag="ACFM8U_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC6333642.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10344..11339)
                     /gene="fbpA"
                     /locus_tag="ACFM8U_000339"
     CDS             complement(10344..11339)
                     /gene="fbpA"
                     /locus_tag="ACFM8U_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC6333643.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11590..12055)
                     /locus_tag="ACFM8U_000340"
                     /pseudo
     CDS             complement(11590..12055)
                     /locus_tag="ACFM8U_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(12113..13489)
                     /gene="argH"
                     /locus_tag="ACFM8U_000341"
     CDS             complement(12113..13489)
                     /gene="argH"
                     /locus_tag="ACFM8U_000341"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC6333644.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(13508..14377)
                     /gene="galU"
                     /locus_tag="ACFM8U_000342"
     CDS             complement(13508..14377)
                     /gene="galU"
                     /locus_tag="ACFM8U_000342"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC6333645.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14405..15004)
                     /gene="rdgB"
                     /locus_tag="ACFM8U_000343"
     CDS             complement(14405..15004)
                     /gene="rdgB"
                     /locus_tag="ACFM8U_000343"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC6333646.1"
                     /translation="MSEKPEKIVLASGNVGKLEEFGNLFKPYGITVLPQSEFGIPECP
                     EPYSTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGDHPKSDTA
                     NNLKLAAELVGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(14997..15230)
                     /locus_tag="ACFM8U_000344"
     CDS             complement(14997..15230)
                     /locus_tag="ACFM8U_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC6333647.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTAGSKAFAVAAFLFA
                     FGALFGQIGALALYLRHKSLHAAQSAAKENRYV"
     gene            complement(15342..15875)
                     /locus_tag="ACFM8U_000345"
     CDS             complement(15342..15875)
                     /locus_tag="ACFM8U_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC6333648.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15977..16435)
                     /gene="nudB"
                     /locus_tag="ACFM8U_000346"
     CDS             complement(15977..16435)
                     /gene="nudB"
                     /locus_tag="ACFM8U_000346"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC6333649.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16646..16722
                     /locus_tag="ACFM8U_000347"
     tRNA            16646..16722
                     /locus_tag="ACFM8U_000347"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16680..16682,aa:Pro,seq:ggg)
     gene            16880..18706
                     /gene="mafB"
                     /locus_tag="ACFM8U_000348"
     CDS             16880..18706
                     /gene="mafB"
                     /locus_tag="ACFM8U_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC6333650.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            18709..19173
                     /locus_tag="ACFM8U_000349"
     CDS             18709..19173
                     /locus_tag="ACFM8U_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333651.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            19398..19544
                     /locus_tag="ACFM8U_000350"
     CDS             19398..19544
                     /locus_tag="ACFM8U_000350"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333652.1"
                     /translation="MWYEANIGLINTMSRSNQAGTRLLYSNYGLLYITTDHYISATRF
                     VAWK"
     gene            19556..19843
                     /locus_tag="ACFM8U_000351"
     CDS             19556..19843
                     /locus_tag="ACFM8U_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFC6333653.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            20357..21601
                     /locus_tag="ACFM8U_000352"
     CDS             20357..21601
                     /locus_tag="ACFM8U_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HFC6333654.1"
                     /translation="MIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEALKKDKQ
                     TARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKESLYYLN
                     KHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGSCAETFG
                     LGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEYVQFSID
                     LFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAH
                     AQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVE
                     IKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAWEKRKELEGQKWRGTSK
                     SGVEIEGFTEPNRTAYPLYNSK"
     gene            21628..22047
                     /gene="mafI"
                     /locus_tag="ACFM8U_000353"
     CDS             21628..22047
                     /gene="mafI"
                     /locus_tag="ACFM8U_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MafI"
                     /protein_id="HFC6333655.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            22137..22208
                     /locus_tag="ACFM8U_000354"
                     /pseudo
     CDS             22137..22208
                     /locus_tag="ACFM8U_000354"
                     /inference="COORDINATES: protein motif:HMM:NF026862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            22205..22447
                     /gene="imm31"
                     /locus_tag="ACFM8U_000355"
     CDS             22205..22447
                     /gene="imm31"
                     /locus_tag="ACFM8U_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC6333656.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            22525..22770
                     /locus_tag="ACFM8U_000356"
     CDS             22525..22770
                     /locus_tag="ACFM8U_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6333657.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            22773..23162
                     /locus_tag="ACFM8U_000357"
     CDS             22773..23162
                     /locus_tag="ACFM8U_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333658.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADLDEEIDNIYNIIGFVSSYSLSKLRRNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            23285..>23655
                     /gene="mafA"
                     /locus_tag="ACFM8U_000358"
     CDS             23285..>23655
                     /gene="mafA"
                     /locus_tag="ACFM8U_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6333659.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSL"
BASE COUNT         5684 a         5404 c         6659 g         5908 t
ORIGIN      
        1 cagataccga acaccaaaca tcaaatatat atatcaggat aaccaaggga cggcagaagc
       61 taggaatacc ggaataaaaa attcaaacgg aaaatatata gtctttattg attcagatga
      121 ttttattaat tgtcaggtct tgctggattt tcttagtaaa gatgatattg atatgccgga
      181 tgtggttttt ttaaatgcgg ttaaatatga taagggaagt gtttcacatt ttggcgaaga
      241 ttatcagcct gaaaaaatac tcaatcaatc caaagtcgaa gttttgaaag gattatgccg
      301 atttagaaaa tttccgggtt cggcgtgtaa taagattata aaaagagaat tgattatcaa
      361 agaaaaacta ttttttgaaa aagaggttta tgccgaagat atcgaatggt caatgagatt
      421 atttaatgcg gcaacaactt tttcttattt ggacggttgt tattactatt atcggcaggg
      481 aagaaaagat tctaaaacag gaactgtttc ggaaaaaagt ataaagtcat tattatatat
      541 tttggagaaa aatgcggaaa tggaatttga tagggatata tcaagttatc tttattcttt
      601 tctttcctac gaatatctcg ttttgctttt tatcatgacg agtaaaaata tagagtgtga
      661 tgctgatata aaaagaaggg cgtatcattt aaggtttatg ctgttaaagt ccaataaatt
      721 gatatataag ctgatattcc cgataatcac attattcggg gtcgatatta caggcaggat
      781 tttaaaagca atcaggggga atatttaata aatcctttaa cactatacat cttactgaag
      841 gaggaaaaat gaacgcaatc cgaactttcc aaaaccgcac gcccgaaatc cacgaaagct
      901 gtatgataga cgaagcgtgc gtcgtcatcg gcgaagtgtc gcttgccgaa gacgtttctg
      961 tgtggccgtg cgccgtgttg cgcggcgatg tgaacagcat caccgtcggc gcgcgcagca
     1021 atatacagga cggcagcgtc ctgcacgttt cccacaaaac cgccgccaaa cccgaagggt
     1081 cgccgctggt tatcggcgaa gacgttaccg tggggcacaa agtgatgctg cacggctgcc
     1141 gtatcggcaa ccgcgtcctg gtcggcatgg ggacgacggt tctggacgat gccgtgattg
     1201 aggacgaagt gatgatcggc gcgggcagcc tcgttccgcc gcgcaaacgt ttggcgggcg
     1261 gctaccttta tgtcggttcg ccggtcagac aagtgcgcgt gctgaccgat gaggaaaaag
     1321 cctttttgaa atattccgcc gcgcattatg tgaagctgtc gaaacagtac gggatgtgaa
     1381 atcacatcgg cgttcctacg tcagccccaa attcacgcgg acgagatgcc cgataacggt
     1441 atccgatgcg ccttgatttt gaccgtctgc gtttgagttg caggcaaaaa tgccgtctga
     1501 agcccttttt tcgggtttag acggcatttt attgccgatt gttttttaaa gtttgaccga
     1561 atgttcgcgc gtttcgtgga acacgatgtc cggccaacgt tcttgcgtca accccaaatt
     1621 cacgcggttg ggggcgaggt aggcgaggtt gccgcccgcg tcgatggcga ggttgcccgc
     1681 gttggctttt tcaaactccg ccagtttttt cttgtcgtcg cacgataccc agcgcgccga
     1741 ccagatggat gcgctgtcga acacggcttc tacgccgtat tcgttggcga ggcgcgaggt
     1801 tacgacttca aactgcaaca cgccgaccgc acccaaaatc aaatccgcgc cgctcatcgg
     1861 tttgaacacc tgtaccgcgc cttcttcgcc aagctgttgc aaaccttttt gcagttgctt
     1921 gattttcagc gggtttttga tgcggacgct gcggaacagt tcgggcgcga agaagggaat
     1981 gcctgtaaat gccagttgtt cgccttcgga gaagctgtcg ccgatttgga tgttgccgtg
     2041 gttcgggatg ccgataatgt cgccggcgta ggcttcttca accagctcgc ggtcgtggga
     2101 catgaaggtt accacgctgg aggcggcgat ttcgcggttg atacgcaggt gtttcatttt
     2161 catgccgcgc tcgaatttgc cggagcagac gcgcaagaag gcaatacggt cgcggtgttt
     2221 cgggtccata ttggcttgga ttttgaagat aaatccggaa aacttcggct cgtccggctc
     2281 gaccatacgc acggtcgcat cgcgtggttt cggcgcgggc gcccattcaa tcaatgaatt
     2341 gaggatttcc tgaataccga agttgttaat cgcagagccg aagaatacgg gcgtgagttc
     2401 gccggcgagg aattcgtcga gattgaactc gttggaagcc gcctgcacca attcgatttc
     2461 gtcgcgcaac tgttggattt ccaatggaaa gcgttgttcc aattcgggat tgtctatgcc
     2521 tttgatgatg tcgaactcgt gcggcaggcg ttcgccgccc gcttcaaaga gatagatttc
     2581 gtcgttcagg atgtggtata cgcctttgaa gtttttgccc ataccgatcg gccaagtaac
     2641 gggcgcgcag cggatttgca ggatgttttc cacttcgtcc aagagttcca gcgaatcgcg
     2701 cacttcgcgg tcgtatttgt tcataaacgt aacaatcggc gtatcgcgca ggcggcagac
     2761 gtttaagagc ttgatggttt gcgcttccac accttttgcc gcgtcaatca ccattaatgc
     2821 gctgtccacg gcggttaaaa cgcggtaggt gtcttcggag aagtcttggt gtcccggcgt
     2881 gtccaagagg ttgacggtgt ggtctttata gtcgaactgc atcacgcttg atgccacgga
     2941 aatgccgcgc tgcttctcga tgtccatcca gtcggaagtg gcgaatttgc cggttttttt
     3001 gccttttacc gtacccgcgc tttgaatcgc acctgaaaac agcaagagtt tttcagtcaa
     3061 cgtggtttta cctgcgtcag ggtgggagat gatggcaaac gtgcggcggc ggcgcacttg
     3121 gtcgaggatt tcttgggaca tggttttctt tgcaaaaagg ttcaggccgc ttttcagacg
     3181 gcccggacag tgtttgagac ggcgaaattg tacaaaaaaa tgcctgataa ttcaatgttg
     3241 gaggcggtca gtgcgtgctg ccgtaaatct ctttttcgtc tttcaggacg gcatcggcgg
     3301 tttcccacgc gctgccacgc cagactttgt aaaagcagct ttctcgcccg gtgtggcagg
     3361 cgatgccgcc gttttgggcg atgagcatca caatggcgtc gccgtcgcag tcgaggcgca
     3421 gttcgcggac tttttgcgtg tgtcccgact cttcgccctt catccattgt ttttggcgcg
     3481 aacggctgta atagtgggca aagccggttt cgacggtttt ttgcagggct tcggcgttca
     3541 tccacgccac cattaaaaca cgtttggttt cggcatcttg ggcgatggcg caaaccaaac
     3601 ctttttcgtc aaatttgacg gcttcaagca ggtttttatc catattttct ttcagacggc
     3661 atggtcgggg cggtcagagg cgcacttcga tgccggcttc gcgcatagcg cgtttggctt
     3721 cgcggatggc gatttccccg aaatggaaaa cgccggcggc aagtacggca tcggctttgc
     3781 cttcggttat gccttcaatc aggtgccgga cattgccgac cccgccggag gcgatgacgg
     3841 ggatgtcgac ggcttcggca acggcgcggg tcagcggcag gttgaaaccc tgtttcgtac
     3901 cgtccctgtc cataccggta agcaggattt cgcccgcgcc gcgtttttgc atttcgatcg
     3961 cccattccac cgcatccaaa ccggtcggat tgcgcccgcc gtgggtaaag atttcccagc
     4021 gtgtgtttcc ggggttgacg gctttggcat cgacggcggc gacgatggct tgcgaaccga
     4081 aaaatccggc ggcttcgtca atcaggtcgg gacgggtaac ggcggcggtg ttgatgctaa
     4141 ctttgtccgc gccggcattg agcaggcggc ggatgtcggc aacggtgcgt acgccgccgc
     4201 cgacggtcag ggggatgaag acttgtccgg caacttcttc gatgatgtgc aggatggtgt
     4261 cgcggttgtc ggatgaggcg gtgatgtcga ggaaggtcaa ttcgtccgcg ccttcgccgt
     4321 tgtagcgttt ggcggcttcg acggggtcgc ccgcgtcgcg caaaccgatg aagttcacgc
     4381 ctttgacgac gcgcccgtct tttacgtcga gacaggggat gatgcgtttt gccagtgcca
     4441 taatcggatg cctttagtcg agggaatctg ccagttgctg cgcttgggca aaatcgatgc
     4501 tgccctcgta aatcgcgcgg ccggtaatcg cgcctgctac gccatgtttt tcggcggcac
     4561 acagggcgcg gatgtcgtcc aagccggtca gtccgccgga ggcgatgacg ggaatgcgga
     4621 cggcttgggc gagtttgacc gtcgcgtcga tgttcacgcc gctcatcata ccgtcgcgcc
     4681 cgatgtcggt gtagatgatg ctgttgacac cgtcgtcttc aaagcgtttt gccaaatcaa
     4741 ttacatgatg cccggttacg gttgcccagc cgtcgatggc ggccataccg tctttggcat
     4801 ccagcccgac aataatcctg ccggggaagg ctttgcacgc ctcgcgcacg aagtcggggt
     4861 ttttgaccgc cgccgtgccg ataatcacgt cgttcagacc caaatccaaa tattgtccga
     4921 tggttttcaa atcgcgtatg ccgccgccga gctgtacggg gatgtctttg gcgacggcgg
     4981 caaggatgtc tttgatggcg ggcaggtttt gcggaacgcc ggcaaacgcg ccgttcaaat
     5041 ctaccagatg caggcggcgc gcgccttgtt tgaaccagtg cagtgcggtt tcggcgggcg
     5101 aatcggaaaa gacggtcgcc tcttccatca gcccttgtct caggcggacg cagcgtcctt
     5161 ctttcaaatc gatggcgggt atcagcagca taatttttct ccttgtgcgg ggccgtgtcc
     5221 ggcttaccag tttaaaaagt ttttcaacat cgtcagcccg gcatcgtggc ttttttcggt
     5281 gtgaaattgc gtggcgaata cgttgtcttt gccgacgatg caggcaaacg gggacgggta
     5341 gtcgctttcg cccaatatgg tttcgggatt ttcgggggcg aaatagtagc tgtggacgaa
     5401 gtaaaaacgc gtgtcttggg gaatatcttt aaacagcggg tggttttggg tttggcgcac
     5461 ggtgttccag cccatatgcg ggactttcag acggcatccc tgcgggtcgc ggaggtcgcg
     5521 ctcaaagcgt ctgactttgc cgccgaacca gcccaagccg tcggtgtttc cttcttcgct
     5581 gtggtcgaat aaaagttgcg cgccgacgca gattccgaaa aagggtttgt tttttaaggc
     5641 atctttgact gcctcgtcca agccgtcgcg cgtcagtgcc gccatacagt cgggcatcgc
     5701 gccctgaccg ggaaaaatga ctttgtcggc gcgggacacg cgttcggggt cgccgcttaa
     5761 aaagatttcg gtattttttc cggcaagctg ccccgccgtc cggacggatt tcaatacgga
     5821 atgcaggttg cccataccgt aatcgataat ggcgatttgc atggcttcct cctctttttt
     5881 tgcaatatgg ctgcgatttt aacaaacaaa tgtgccgtgc tgataaaaat gccgtctgaa
     5941 aacgggagtc tgtcttcaga cggcataggg tttaaacccg gaaagccgtt tgtcagcctt
     6001 ccatttgttt tgcctgaacg gcagtcaggg cgatggtgaa cacgatgtct tccaccagcg
     6061 cgccgcgcga caggtcgttg accggtttgc gcaggccttg cagcagcggg ccgacgctta
     6121 agacgttggc actgcgttgg acggctttat aggtgcagtt gccggtgttc aggtcgggga
     6181 agaccaaaac ggttgcctgt cctgccaccg ggctgcccgg agctttggat ttgcccacgc
     6241 ccggcacggt tgccgcatca tattgcagcg ggccgtcgat ggcgaggtcg gggcgttttt
     6301 cccgggcaag tttggttgct tcgatgacga tatcgacatc ggggccgctg ccggagttga
     6361 cggtggagta ggaaatcatc gccactttcg ggtcgatgcc gaaggctttt gcggaatcgg
     6421 cagactggat ggcgatgtcg gcaagctgtt gcgcggtcgg gttcggatta accgcgcagt
     6481 cgccgaagac gaggacttgg ttgggcagca gcataaagaa tacgctggac acgaggcttg
     6541 cgcccggtgc ggttttaatc agttgcaaag cggggcggat ggtgttggcg gtggtgtgaa
     6601 ccgcaccgga taccaaaccg tccacatcat tttgcgccat catcatcgta ccgagtacca
     6661 cggtgtcttg cagttgcttg cgcgcgtctt cgggtgtcag gcctttggat ttgcgcagtt
     6721 cgcacatcgg ctcgacgtat tgttcgacca atgaggcggg atcgatgatt tccaaagagt
     6781 cgggcaggct gatgccgcgt tctttggcaa cggcttcgac ttcttcgcgt ttggcaagca
     6841 ggacgcagcg ggcaatgcct ttttcgtggc agatggcggc ggcttggacg gtgcggggtt
     6901 ctgcgccttc aggcaggacg atgcgtttgt cggcttggcg ggcgaagtcg atcaggttgt
     6961 agcggaattg cgccggcgac aggcgttttg cttcgcggcc tgccaatacg gatacgtctt
     7021 tcagcgcgtc gctcgaaccg aagaaggtca ggccggtttt ttcggctgcc gcttcggcaa
     7081 cggaggctgc cgcgccgtcc acgacaaaac cttccaatac gcccggcgcg gcggcgaaga
     7141 actgtttggc aaggttcacc cgatttgcca gttcgtcggc atcggcgttg tcggaacgga
     7201 cggcgaagac ggctgccgcg tcaagggaca atgccagttc gacgtttttg cctgcgaggt
     7261 agattttgtc ggcatcgggc gcgatgcctt cgatgacgag gttggcggca tcgagtgcgg
     7321 caactttgcc gaccagtgcg tcgaaccagt cgtcgctttt gccttgcgcg agcagggttt
     7381 cggcggttgc gtcaacggct tggaaaattt gtgcgtccag tgcttttgca aaggcttgtg
     7441 cggcggcgga ggcgtccagt ccggcagata cgggtacgat gagtactttt gccatgatat
     7501 atcctttcgt atgctgcggt gtgcggcata tgtggttgga aggggcggca tataggcaga
     7561 aacggctgcc tgcgtgccgt gcgtgccgtg tttggcttga ggcgcgcagg ttgaatatag
     7621 caaacaaatt ctgtttccaa caagataaat atccgcaggc ttgtggatgc tgccgccttt
     7681 cagagggtat ttccggggaa gaacagggcg ggaccgtcca aatggaggac ggcggaaatg
     7741 ccgtctgaca gggtgggggc ggaagggagg ttgagcgtga ggacggtttg tccgacccgg
     7801 aggctgattt cggtatgccg cgctttgggc gtggttttga gaaccacggc gtgaatggag
     7861 gcggtgggtg cggaatgggg gtgaaggctg aactgttccg gacggatgag cagtgtgccg
     7921 cgtgtgcctg cgggtgcgcc gctttgaacg ggcaggcggc ccaatccgca atcggcggtg
     7981 ccgtcggcgt tgagcgcggc ggggaacacg atgccttcgc cgataaacag ggcggcatca
     8041 aggtcggcag gttgtcggta caattcgtga gggcttgcag tttggaggat gcgcccctgt
     8101 ttcatcacgg caatccggtc ggcgtattgc agggcttctt cgcggtcgtg gctgacgaaa
     8161 acggcagatt tgccgttggc gcgcagggcg gcaatcatgt cttcgcgaat ctggcggcgc
     8221 aactgttcgt ccagcgcgct gaagggttcg tccaacaaaa tcagttcggg atcgggtgcg
     8281 agggcgcggg cgagggcgac gcgctgttgc tgtccgcccg aaagttcgtg cggatagcgt
     8341 ccggcaagtt cggaaatgcc ggtcaattcc aacatagctt cgatgcgctg ccgctcttgc
     8401 gccgtcttgc ctttgccgtt gcccagcccg taggcggtgt tgcggtaaac ggtcaggtgg
     8461 gggaacagca caccttcctg tacgacataa cccaaacggc gttcgcggac ggggaggttg
     8521 gtatttttcg agaagatggt tctgccggaa agcgaaattt cgccaaaatc gggttgttca
     8581 aaaccggcaa ggcagcgtaa aagggtggtt ttgccgcagc cggacgcgcc gacgataaag
     8641 aggatttcgc ccgggtcgag gctgagcgaa atgtcgttta aaaccggggt gttttgaaaa
     8701 cttttggaca ggtgtccgat gtgcagggcg gcggtcatgg cggtacttcc tcaagctgtt
     8761 atttgaaggc gtatttcttc agcaggaata cggggatgcc ggaaaataat accagcatca
     8821 gcgcgtaagg ggtggcggcg gcgtattgtg cgtctgatgt gtattcccaa acggcggtgg
     8881 agagtgtgtg gacatcgtcg gtggtcagca gcagggtggc ggtcagctct ttcatcagtt
     8941 tgaggaagac gagtgcgaat gcggcggtaa tgccgggcag gatggacggc agtaccaacg
     9001 tcctgaaaat aaagaagtgt ccgcgcccca atgttgcgcc gacctgttcc atcccttttg
     9061 ggagttgttc caaggaagtc ctcagggtgg tttgcgccat cggcaggtaa agcatgaaat
     9121 aggcaaggat gacgacgata aaggtttggt aaacggcagg ggtgtagttg atgctgaaat
     9181 aaaccaagga tagggcgata accaaaccgg ggacggcgtg cagtaaaaac ggcagcctgt
     9241 ctatccaaac ggttaaaaaa ttgcgatagc gaaccgatgc ccaaacaagg ggcaaggcac
     9301 ataatatagt caaaatcgca cctaaagccg atacgcttaa ggaacggata aaggcatcaa
     9361 atacggatac gagcgcgaat gtgccggaag tgccgaccat catccaatgt atcaatacgc
     9421 caaaggggat aataatgccc aaagtcaaca agctgcttaa aaaaacaatc gcgccgatct
     9481 gaccgggcag tttgagggtt ttgacgggat aaggacgggc aacgcctttg ccgctgtggt
     9541 aaatcttggc tttgccgcga aatatgcttt ctccaaatac gacgatgccg cacaccgcca
     9601 ttaaaacagc ggaaagcagg gcggcggtat tgttgttgta ggacatttcg tattcttgga
     9661 aaatggcggt ggtaaaagtg gggtagttca aaatggatac cgcgccaaat tcgaccagca
     9721 tatgcagggc aatcagtaac acgctgctgc cgatggcggg tttgagctgg gggaggatgg
     9781 cggaaaaaaa ggtttgcagg cggcttttgc ccaaggacag gctgacttct tcgtaagaca
     9841 ggctgatgcg tttgagtgcc gcctcgacgg gcaggtaggc gagcgggaac gaggacaggc
     9901 tcataatcat cactgtcccc caaaagcctt cgacacggaa ggtcaggctg atccaggtga
     9961 aacagctgac aaatgcgggg atgcacaaag gcagggtgat tgccgtctga aaaaaggttt
    10021 tgccgaagaa gcggtaacgt tggaacaaaa gggcgcaggc aatgcccaaa acaatggaaa
    10081 tcagggtaac gcccgccatc atcgtcaagg tgttggagag caaatcccac atacgcgggc
    10141 ggaacaacag ttcgacggcg cggttgatgc cgacctgcca cgaacgcata gcgacatata
    10201 aaaaaggcag ggtaagcggt agggcaatca gtaggatgag gccggtaagc caaatgggta
    10261 tttttttagg agacatagtg ttttttatcg gcaaaacggg cggacagtat aaatgtccac
    10321 ccgtttgaca atccgaaaac ggcttatttc ataccggctt gctcaagcag ccgggtggcg
    10381 tgttcttttt cggaaacagt ggtggcggac acttggggtg cttccaactt ggcgatgggt
    10441 tccaaattga aggtggatac cacgtgcgga ttcaaaggat attcggcacg gacggcggtc
    10501 agggcacgct gtccttcctt gctggcgagg aaagcgacga attttttcgc ctcatccttg
    10561 ttttgggagg attttaacac ggctgcgccg gaataggtaa cgagtgcgcc gggatctctg
    10621 tggcggacga aattcaggcg ggtgtggaca ttttgtacgc ctttttcacg cgcgaaagcg
    10681 tgccagtagt agttgttgat gagggcggca tcgatttcgc cgttttcaac cgcttgaagg
    10741 gcgacggagt ttttagcgta aggcttgccg tattctttca gacctttgag ccatttcaat
    10801 gcggccgctt cgcctttcag tttgacgatg gcgacaacct gttccaagaa cgcgccggaa
    10861 gtgggggcgt aaccgatgcg gtttttccat ttcggcgtgg cgtaattcag gacggatttt
    10921 tccaaatctt tttcagacag tttgcgggtg tcgtaaacga cgacgcgcga acgtccgctc
    10981 agtgccaccc agtctttttt ggcggcaacc ggcacgccct tgccgcgtgt ttcgttgatg
    11041 gtggaggcgg gcaggggctc taggaggttg gctgcggaaa gggtggcgag tgccgggatt
    11101 tgttcggaat agaatacgtc ggcggggctt cggctgcctt cttctttgat ttgaccggca
    11161 agctggtcgc ctttggcgct gttgagtttg actttgatgc cggtagcccg ggtaaaggca
    11221 tctgcaacgg cttgtgctgc ttctttgtgt tgtccgttgt acacggtaat gtctgccagc
    11281 gcgggggttg cggcggtcag ggctgcggca agcagtgcgt atcggataga tgttttcata
    11341 tcgattttct cctaatgaat gagagtgtac accttgttaa gacataacgg cgtgtagtgt
    11401 attccttctt ctttataaat gcaaataatt attttttaaa tttgttatta tccgatccgg
    11461 ttatggtttg ttctgacttg tattttttcc gtgcatcgcg cccgtaaggc ggaagtggcg
    11521 ggcaatgcgt ggcggaatgt gggtaaaggc ggcattttga tttgtcggaa tgcttgagaa
    11581 cccctctctt taaaacaccg tctgaacagg gttgctggaa tagtattgcc atcccagcag
    11641 atacagtttg tcggggtctt gccaatattg ttcatccaga ctgttcggca gtgaggcggt
    11701 tttgttgtcg agatgttata tcccacattt ctttcaggtt tttaccttcc gattggaggc
    11761 ggcggatttc tttgtccaat gcgtctgccg cccgatggat cagcattgcg ctgtgtatcg
    11821 cgccgatttt tttcagaacg gaactagtct tttcggcggc gggaaaacac cagttgcaga
    11881 caaattgcag tatgccgccg ttaccgatgt cggcttccgc ccgccaaatc aaaaccagct
    11941 cctgttcccg tccgtccatg ctttcgagtt tgccgttatg ctgttcaaaa agtttgtcca
    12001 ccgcctgcca catcatctgc tcgaagcggt cggcttgggt atccgtatcg gtcatcgtgt
    12061 tcttgccttt taaaaatgcc gtctgaacat ttcttcagac ggcatttggg ggttaagcca
    12121 acatttcccg ccagcgtttc acttggaagc ggacttgttc cggcgcggta ccgcccaagt
    12181 ggttgcgggc gtttaagctg ccttcgggtg tcagcacgcc gtaaacgtcg tcggcaatca
    12241 aatcgctgaa accttgtaag acttcgagcg gcagttcgct caaatcgacg cccgcttggt
    12301 cggcgtggcg cacggcttgg gcgacgactt cgtgggcatc gcggaaaggc atgccttttt
    12361 tgaccagata atccgccaag tcggtggcgg tagcgaagcc ctgcatcacg gcggcgcgca
    12421 tattgtcggg tttgacggtt acgccgcgca tcatatcggc gtaaatccgc aacgtgtcga
    12481 taagcgtgtc ggcggtgtcg aacaagggtt ctttgtcttc ctgattgtct ttgttgtacg
    12541 ccaagggttg ggatttcatc agggtaatca gaccgataag gtgtccgatg acgcggccgg
    12601 atttgccgcg cacgagttcg ggcacgtcgg ggtttttctt ctgcggcatg atggacgaac
    12661 ctgtgcagaa acggtcggcg atgtcgataa agccgaaacg cggactcatc cacaaaatca
    12721 attcttcaga caggcggctc aggtgaacca taaccagcga ggcggcggcg gtgaactcga
    12781 tggcaaaatc gcggtcggat acggcatcga gcgagttttg gcagatttgt tcaaagccca
    12841 acagttcggc ggtgatttcg cgccgaatcg gataagtcgt accggcaagg gcggctgcgc
    12901 cgagcggcat acggttgacg cggcggcggc agtccgccat ccgttcgtta tcgcgtccga
    12961 gcatttcgac gtaggcgagc atatggtgtc cgaagctgac gggctgggcg acttgcaggt
    13021 gggtaaagcc tggcatgacg gtttcggcgt tttgttccgc caaatccagc aatgccgtct
    13081 gaaggctttg aatcaggctt cggatgacgg taatctggtc gcgcagccaa aggcggatgt
    13141 cggtggcgac ttggtcgttg cggctgcggc cggtgtgcag gcgtttgccc gcgtcgccga
    13201 ttttgtcggt caggcggcgt tcgatgttca tgtggacatc ttccaaatcg gacgaccatt
    13261 cgattttgcc gctgcggatt tcttcgagga tttccgccat accccgacgg atgtctgcca
    13321 aatcgccttc gtccaacacg ccggtttctt tcaacatttg cgcgtgtgcc agcgagcctt
    13381 gaatgtccca ttcggcaagc cgtcggtcga aaccgatgga ggcggtgtat tgtttgacga
    13441 gttcggaaac gggttcgttg aaacgtccgg accaggtttt gtcgtgcata aggattcctt
    13501 gatggggtta ttcggtacgg tatttttcca aaaggcggcg gaagggttcg ccggtttcgg
    13561 ggtgtttcag accgtaggcg acggtggctt cgaggtagcc cagtttgctg ccgcagtcgt
    13621 agcgcgtgcc ttcaaagggg tgcgccagga caaattcgtg atcgagcagc ttggcgatgc
    13681 cgtctgtaag ctggatttcg ttgcccgcgc cgcgcggcag tccggtcagt aagtcgaaaa
    13741 tgcgcggggt gaggatgtag cgtccaacaa cggcaaggtt ggagggcgcg tcttcgggct
    13801 tgggtttttc gacaatgccg gtaatgcgtt ggaactgttt gagctgttcg gtttcgacga
    13861 tgccgtatga gccggtttgc gatgcttcaa cggtttctac gcccaaaatg ctgttgccgc
    13921 tgcgcccgta cacttcgacc atttgtttga gcgcgccttt gggggcatca atcaggtcgt
    13981 cggcaaggat aacggcaaag ggttcgtctc cgatggcggc gcgggcgcac aagacggcgt
    14041 gtcccaagcc cagtgcttcc gcctgacgga tgtagaggca ggtaatgttc ggcggcagga
    14101 tgttgcggac gtgttccaac aatttgtctt tatggcgcat ttccaactcg gtttcgagtt
    14161 cgtatgcctt gtcgaaatgg tcttcgatgc tgcgtttgtt gcgtccggtt acaaacacca
    14221 tttccgtgca gccggcttcc acggcttctt ccacggcgta ttggatcagc ggcttgtcga
    14281 cgatgggcag catttctttc gggcttgcct tggtggcggg caggaagcgg gttcccatcc
    14341 ctgcgacggg gaagacggct ttccgtatgg gtttcattct ttttcctttg tattgttttg
    14401 atgtttaaag ggcgagtttg cgtaagagtt cggcaagtgc ctgcgcgcgg tggctttcgc
    14461 ggtttttgac ctccgaatcc aattcggcgg cggttttgcc gtgtccgggc agataaaaat
    14521 acgggtcgta accgaaaccg ttttcaccgg cgggaacgcc gttccattgt ccgtgccata
    14581 cgccttcggc aataatcgga cgcgggtcgt ctttatggcg gacaaaaacc aatacgcaga
    14641 cgtagcaaca gcttttgtcg gctttgccga caagttcggc ggcaagtttc aggttgttgg
    14701 cggtatcgga tttgggatgg tcgcccgcgt aacgtgcgga atgtatgccc ggtgcgccgt
    14761 ttagggcggc ggcacagatg ccgctgtcgt cggcgagtgc gggcagcccg ctgtattcgg
    14821 cagcatggcg tgcttttgcc agcgcgtttt cgacaaaggt ggaatagggt tccggacatt
    14881 cgggtatgcc gaactcggat tgcggcaata cggtgatgcc gtaaggtttg aataggttgc
    14941 cgaactcttc gagcttgccg acattaccgc ttgccaaaac gattttttcc ggtttttcag
    15001 acatagcggt tttcctttgc ggcggattgg gcggcgtgta gggatttgtg ccgcaaatag
    15061 agggcgagtg cgccgatttg tccgaacagc gcgccgaatg cgaaaaggaa ggcggcgacg
    15121 gcaaaggctt tgctgccggc ggtcagcaga taagcaccca agaggacgag cagcataaat
    15181 gccagtgtga acagggcaac agacaacaga tagatttttc ggcggttcat ggcgttcggt
    15241 cggaaacggt atgttcggat tatatccgat tgggacggta ttccctagag cttggaaaaa
    15301 tgccgtctga agcggctttc agacggcagg gcaggcggat gttatgcctg tttgttccaa
    15361 cgttcgatgg attctttaat aacttttttc gcttcttccg catcgcccca ggattcgact
    15421 ttggtcgtac cggctttttt caggtctttg taatggttga agtggaactc gatttgtttg
    15481 atgagctgct gcggcaaatc ggacaaagtt ttgtaggcgt tgccgttgtt gcggtcgtcg
    15541 gcaggaacgc agacgatttt gtcgtctact tcgccgtcgt caacgaattt catcacgccg
    15601 ataacgcgcg cttccaagaa tacgccggtt gccagaggtt gttcggtaac gagcagcacg
    15661 tccaattcgt cgccgtcttc gtccaaagtt tggggaatga agccgtagtt ggtcggtttg
    15721 gcgaagatgg cgggttcgac gcggtcgagt tggaatgcgg cgagtttgcg gttccactcg
    15781 attttgtggt tgctgccggc ggggatttcg ttgacgacgt tgatgatgcc gccgtccacg
    15841 tcgccggggg tcaggatttg gttgaagtct gccatttggt ttcctttatt tgggaaagtt
    15901 tgaagtttga aagtatagca caaacgtccg gctgaaaatg cgcccgatgc cgtctgaaag
    15961 ggtgtacggg cgcgtgttac cgtttgccca aaaacctgcc cagttccaga atcgcgcgcc
    16021 tgttggacgg ggaaaacact ttttccgccg cttcttccaa gccgaaccag ccgtaggaga
    16081 cgtgttcctc gggttgcagg acgacgggcg tatcgcgcgg gatttcggca gagaagaggt
    16141 gttcgcggtt ttcaaacacg ccttttggat agcggtgccg ccagtggtgg tagatttcgt
    16201 aaaccgtgct gtcgtgccag tcttgaagct gcccgtccgc cagcaggatg ccggtttctt
    16261 cccaaacttc gcgccttgcc gtttgggcga cggtttcgcc cggttcgagg ctgccggtta
    16321 ccgactgcca aaatccttcc ggatgcgtgc gttcgatgag caggatgccg ccgtccccgc
    16381 tataaaggac gaccagtgcg gaaacggggt atttgagcgg ttttgccatc ggcatctttc
    16441 ggcgggctgc ggtaatgaag gggcggatta tagcaaacgc cgcacgttat ggcgtttatc
    16501 cttttccgta tccttttttc tgcacggatg ggacgcgccg gtgtttgccg gtaaattttc
    16561 cgttgtgtca aaaagataag ggcggttgtg attttaatgc ttgccaaagc gtcgggcgga
    16621 aactataatc cgaaacttat cgagtcggag tgtggcgcag tctggtagcg cacttgcatg
    16681 gggtgcaagg ggtcgaaggt tcgaatcctt tcactccgac caaaaattcc gaaagccgct
    16741 ttcaaaagcg gcttttttgc cgtccgtatg attatgatgt agagtacgcg gcgacagaca
    16801 ttcaaatgcc gtctgaaaac cgttcagatg gcatctcttt atcttagttt cattccgtac
    16861 catcttaagg aacatcaaat tgggcatttc ccgcaaaata tcccttattc tgtccatact
    16921 ggcagtgtgc ctgccgatgc atgcacacgc ctcagatttg gcaaacgatt cttttatccg
    16981 gcaggttctc gaccgtcagc atttcgaacc cgacgggaaa taccacctat tcggcagcag
    17041 gggggaactt gccgagcgca gcggccatat cggattggga aacatacaaa gccatcagtt
    17101 gggcaacctg atgattcaac aggcggccat taaaggaaat atcggctaca ttgtccgctt
    17161 ttccgatcac gggcacgaag tccattcccc cttcgacaac catgcctcac attccgattc
    17221 tgatgaagcc ggtagtcccg ttgacggatt cagcctttac cgcatccatt gggacggata
    17281 cgaacaccat cccgctgacg gctatgacgg gccacagggc ggcggctatc ccgctcccaa
    17341 aggcgcgagg gatatataca gctacgacat aaaaggcgtt gcccaaaata tccgcctcaa
    17401 cctgaccgac aaccgcagca ccggacaacg gcttgccgac cgtttccaca atgccggtag
    17461 tatgctgacg caaggagtag gcgacggatt caaacgcgcc acccgataca gccccgagct
    17521 ggacagatcg ggcaatgccg ccgaagcctt caacggcact gcagatatcg ttaaaaacat
    17581 catcggcgcg gcaggagaaa ttgtcggcgc aggcgatgcc gtgcagggca taagcgaagg
    17641 ctcaaacatt gctgtcatgc acggcttggg tctgctttcc accgaaaaca agatggcgcg
    17701 catcaacgat ttggcagata tggcgcaact caaagactat gccgcagcag ccatccgcga
    17761 ttgggcagtc caaaacccca atgccgcaca aggcatagaa gccgtcagca atatctttat
    17821 ggcagccatc cccatcaaag ggattggagc tgttcgggga aaatacggct tgggcggcat
    17881 cacggcacat cctatcaagc ggtcgcagat gggcgcgatc gcattgccga aagggaaatc
    17941 cgccgtcagc gacaattttg ccgatgcggc atacgccaaa tacccgtccc cttaccattc
    18001 ccgaaatatc cgttcaaact tggagcagcg ttacggcaaa gaaaacatca cctcctcaac
    18061 cgtgccgccg tcaaacggca aaaatgtcaa actggcagac caacgccacc cgaagacagg
    18121 cgtaccgttt gacggtaaag ggtttccgaa ttttgagaag cacgtgaaat atgatacgaa
    18181 gctcgatatt caagaattat cggggggcgg tatacctaag gctaagcctg tgtttgatgc
    18241 gaaaccgaga tgggaggttg ataggaagct taataaattg acaactcgtg agcaggtgga
    18301 gaaaaatgtt caggaaataa ggaacggtaa taaaaacagt aactttagcc aacatgctca
    18361 actagagagg gaaattaata aactaaaatc tgccgatgaa attaattttg cagatggaat
    18421 gggaaaattt accgatagca tgaatgacaa ggcttttagt aggcttgtga aatcagttaa
    18481 agagaatggc ttcacaaatc cagttgtgga gtacgttgaa ataaatggaa aagcatatat
    18541 cgtaagagga aataataggg tttttgctgc agaatacctt ggcaggatac atgaattaaa
    18601 atttaaaaaa gttgactttc ctgttcctaa tactagttgg aaaaatccta ctgatgtctt
    18661 gaatgaatca ggtaatgtta agagacctcg ttataggagt aaataaaaat ggcaatttgg
    18721 aattatcggt actaccttat cccatcagct gctatcagaa ataaatttaa tgcaaataaa
    18781 gatatcatac ttgatgagta tcgatctaat ggttttcaga attttaatga gaataaaagt
    18841 tttgaaaatt actttatcga taatgatgtt atattattat caataataaa tgaagcaaaa
    18901 aaacagctta aattgaaaga atcttgggat aaagacgcaa tcatgttttg tgataatttt
    18961 ggtaatagtc ttaccgtttg gccagatgat atagagtgcg aacttgattt aagatttgat
    19021 tatactaaat ttattcagaa aaccattgat tgggcaataa aatataattg tctacttgta
    19081 atagaaaaaa caggaaatgt agtttcccct aatataaata atctgatgta tgaaataaaa
    19141 gcatatttgg aaagcaagcc gtggcccata tgaaacctaa actcaacaag taggatgtgt
    19201 gcggaacgca cgtatgcggt tctcaaggtt tgagctaaga ggccgtctga aaacagaaaa
    19261 actgtttcag acgacctttc ttttaaccag ttgccacagc aaccggacaa aagcagccta
    19321 cctccacatc catataggca atacagggga gatattttgt aaattctacg aatattttac
    19381 ctgctaaaca gggtaggata tggtatgaag cgaacattgg cttaataaac actatgtcaa
    19441 gatcgaatca ggctggtact agattgttgt attccaatta tggattgcta tatataacaa
    19501 ctgatcatta tatctctgca acacggtttg tagcttggaa ataggagtat aacttatgca
    19561 attagagatt atcggtagta aaatttatac ggaacaagat tttcataatc aaatttcaaa
    19621 aatattttct atacaagatt attatgggaa caatcttgat gctttatggg atttattaag
    19681 cacaaatgta gaacgaccga ttactttggt atggaaagat gctatgttct caaaaaatca
    19741 attagaaaat atatttattg aaatcgtaaa tgttctagaa agagttaaga aacaagatga
    19801 ggattatgga ttcgaagaaa aatttaatta tattttagag taagtaaacc ctaattacat
    19861 tacgtacaca ggcttaaaac tccccagggc caattaagca agccgtaacc catataaaac
    19921 ttaaactcaa caagtagcat gtgtgcggaa cgtacgcatg cggttcttaa agtttgagct
    19981 aagaggccgt ctaaaaacag aaaaaccgtt tcagacggtc tttgtttaac gccaccgatc
    20041 cagcgggtta caaagcgcag tcaatgccgc tgcgccttat gcctccgaag caataggcag
    20101 aacatttgga cacggtgaaa acaaaaacga aaccgcccaa gccgttggac atttcctttt
    20161 aggagcagct attgcccgcg tcaacggtgg taattttgct gccggcggct cggcagcagt
    20221 tgcagctgaa aaggcggcgg aacatcttgc ccaacagtat aacgacggta aaaccgcaat
    20281 cgatccgcaa acaggcgagt tcaatgccaa cctgctgccg gaacatatca aagaggaaat
    20341 caaatcaaag agcggggtga ttgcatcgct gacgggcgcg gccgtgggcg gcacgccggt
    20401 agatgcgcaa accggaggtg cggtcggaca gaatgcggtg gaaaacaacc tctatctgac
    20461 atcggaagcc ttaaagaagg acaagcagac agctcgtaaa atttattccg tcataaaaga
    20521 gcaagtcaag catgaatgca gttccacagg aagaattacc gaatgtcgtc aaaatatagg
    20581 acgcattatc gaatttaccc aagacaaacg ctttgacagt aggtttaagg acttaaaaaa
    20641 agaatcctta tattacctaa ataaacatcc tgatttagta gcctcttatt tgaaggctga
    20701 atacgaaaag ctggataggg aagacaaaag tatcctgcac cgctacatct cacccggggc
    20761 tgaaatcgtt tcgggcagtt tgggggttgt tctttcagga gtagccggag gcggatcttg
    20821 tgccgagact ttcggcttag gctgtgccgc cgctttggtt ggtgtaacgt cttcctacga
    20881 tcatgtcatt actgggacga aaaacttcgg aaaaaaagcc agcgagcaac gaccgacgat
    20941 tgcggttcag gccttgaagc agttggggct gtcggagcag gctgcggaat atgttcagtt
    21001 ctctatagat ttgttcagtg tgggtaaatc ggggggcggt atacctaagg ctaagcctgt
    21061 gtttgatgcg aaaccgagat gggaggttga taggaagctt aataaattga caactcgtga
    21121 gcaggtggag aaaaatgttc aggaaacgag aagaaggagt cagagtagtc agtttaaagc
    21181 ccatgcgcaa cgagaatggg aaaataaaac agggttagat tttaatcatt ttataggtgg
    21241 tgatatcaat aagaaaggca cagtaacagg agggcatagt ctaacccgtg gtgatgtacg
    21301 ggtgatacaa caaacctcgg cacctgataa acatggggtt tatcaagcga cagtggaaat
    21361 taaaaagcct gatggaagtt gggaaatgaa aaaaggaagg ggcggaaaac ctatgacaaa
    21421 tcattctatg ttcccaaaag attgggatga ggctagaatt agggctgaag ttacttcggc
    21481 ttgggagaaa agaaaggaat tagaaggtca aaaatggaga ggtacaagta aatctggtgt
    21541 tgaaatagaa ggatttacgg aacctaatcg aacagcttat ccactttata atagtaaatg
    21601 aagtattgat taaaaaggtg tttttaaatg tataaaataa aatgttttta tgatcagttt
    21661 agttctggtg aattatttaa ctactgccag tttttagata attcttctaa tcaaaagata
    21721 aatacacgag gaacggtgta tcaatatatt atttatgttt taacaggaga cctttatcta
    21781 caaaaagaca tagatgaaaa tttagaattt atccatcaag cagaaaataa tcccaatgaa
    21841 gtttattcag gaggtggaca aggtttttgc tgggatattt ctgctgaaaa agtagttttc
    21901 tatcacaacg aatttgatga agaagacggc tggcctgatt taagctgttc gctgcatact
    21961 tttaaaactg ctttaattgc ttggaatgct tttttgcaat tgcctaaaag tattcattcg
    22021 gtggtggaga ctgtgattga ggaataagca taattagctt aatgaataga atcagcgata
    22081 tagattggac tgcaaatcca cgcttatacg ctgtgccatg attaacagga agagagcaac
    22141 ttggatgggc tagtagggat ggtaagcatt taaatatatc aattgatgga aagattacac
    22201 acaaatgact aatttaaaat tagatttcta ctctgaagtt attataaaag attcttgccc
    22261 taatgatttg ttagaaaatg gtgagactat taaaggaaaa aaaggagtgg tattaggtat
    22321 aagcgaagaa gatggtataa tctatggcta tactatttta ctttttgata taaaatattg
    22381 tatatatata gataagaaat atattatacc gactggaaaa aaattctctc gcgatgattt
    22441 ttattagcta atttaagtaa tttaagcaag ttttttagat actgaaactg gtaattatgt
    22501 ttatgtaagc ataaggagtt cagattgaag gattctacag ggcaaatgag gctggcaacc
    22561 aaggatttgg cggaagccat taaacgagga gaagtacgta gttctgcttt tacaacaaag
    22621 caactaaagg caatcgaaaa aggagaatct aaaataccgg attacacttg gcatcatcat
    22681 caagacactg gaaggatgca attgattaat gaagacttgc atcatgatac cggccatatc
    22741 ggttggagag cgatgagtaa aggaaagtaa ctatgtggaa aatcataaaa gaggatagtg
    22801 atgatttagg atttgcaatt aaatgcttat tctctcaatc tattgattta aatgaattca
    22861 aattatggat tgaacaagta atacgcgata tgcccattga ggacatccct ttttatattt
    22921 ttgatttggc ggatttggat gaagaaattg ataatattta caatatcata ggttttgttt
    22981 cgagttacag cttatcaaaa ttgagaagaa atgccttaac aggcattgcc ttcttaaggg
    23041 ggatagatgt ctatgaccct cccgtttcaa aagaaaaagc attaaaagcc ttagagaaat
    23101 atcctgaaat ttatcagaaa tttcagcatt tctttccgtt tgtagagctg ccgccgcttt
    23161 aaacagtcaa aatgccgtct gaaagccatt tccgccgctc agacggcatt ttcgcccctt
    23221 ttgtttacaa acccttaaaa tccctttaca ctcaaaatcc gttcaacatc aaacaaaccc
    23281 cgctatgaaa accctgctcc tcctcatccc cctcgtcctc acagcctgcg gcacactgac
    23341 cggcataccc gcccacggcg gcggcaaacg ctttgccgtc gaacaagaac tcgtcgccgc
    23401 atcgtcccgc gccgccgtca aagaaatgga cttgtccgcc ctgaaaggac gcaaagccgc
    23461 cctttacgtc tccgttatgg gcgaccaagg ttcgggcaac ataagcggcg gacgctactc
    23521 tatcgacgca ctgatacgtg gcggctacca caacaacccc gaaagtgcca cccaatacag
    23581 ctaccccgcc tacgacacta ccgccaccac caaatccgac gcgctctcca gcgtaaccac
    23641 ttccacatcg ctttt
//
LOCUS       DAXHSJ010000012        23121 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000012 DAXHSJ010000000
VERSION     DAXHSJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23121
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.012"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(93..932)
                     /locus_tag="ACFM8U_000359"
     CDS             complement(93..932)
                     /locus_tag="ACFM8U_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC6333660.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADGAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1217..1292
                     /locus_tag="ACFM8U_000360"
     tRNA            1217..1292
                     /locus_tag="ACFM8U_000360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1250..1252,aa:Arg,seq:ccg)
     gene            complement(1357..2550)
                     /locus_tag="ACFM8U_000361"
     CDS             complement(1357..2550)
                     /locus_tag="ACFM8U_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC6333661.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="ACFM8U_000362"
     CDS             complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="ACFM8U_000362"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC6333662.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSISAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3802..4902
                     /gene="aroC"
                     /locus_tag="ACFM8U_000363"
     CDS             3802..4902
                     /gene="aroC"
                     /locus_tag="ACFM8U_000363"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC6333663.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            4993..5418
                     /locus_tag="ACFM8U_000364"
     CDS             4993..5418
                     /locus_tag="ACFM8U_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC6333664.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5492..7477)
                     /gene="parE"
                     /locus_tag="ACFM8U_000365"
     CDS             complement(5492..7477)
                     /gene="parE"
                     /locus_tag="ACFM8U_000365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC6333665.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTRTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVAGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7544..8092)
                     /locus_tag="ACFM8U_000366"
     CDS             complement(7544..8092)
                     /locus_tag="ACFM8U_000366"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC6333666.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINNRNEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQSSDNR"
     gene            8234..9529
                     /gene="serS"
                     /locus_tag="ACFM8U_000367"
     CDS             8234..9529
                     /gene="serS"
                     /locus_tag="ACFM8U_000367"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC6333667.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDTLEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMNQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYEALEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9607..10605)
                     /locus_tag="ACFM8U_000368"
     CDS             complement(9607..10605)
                     /locus_tag="ACFM8U_000368"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6333668.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDVKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGKYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10730..11806)
                     /gene="prfA"
                     /locus_tag="ACFM8U_000369"
     CDS             complement(10730..11806)
                     /gene="prfA"
                     /locus_tag="ACFM8U_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC6333669.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11896..12675)
                     /locus_tag="ACFM8U_000370"
     CDS             complement(11896..12675)
                     /locus_tag="ACFM8U_000370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6333670.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAAREAGVGRFIVPATRPQDW
                     QDVADLAQRLSENPLWGNIHIALGIHPWFSDDVSEPDFQRLEQALKARPMAWVGEIGL
                     DFYDKTQTPQQRERQIQVFSRQLAIAQTLRRRVIIHNLKASNEIAAAVRQTGFSCGGI
                     VHAFSGSIEEARVLVGLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLK
                     NTVNTPANILRIAEIAAQIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12790..13779)
                     /locus_tag="ACFM8U_000371"
     CDS             complement(12790..13779)
                     /locus_tag="ACFM8U_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC6333671.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIHYPDPQAKIAVRTLIPG
                     FAVQELADGLGRLSAQALILQSYGHGNTPADEGFIRAVRDFTRQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13846..14529)
                     /locus_tag="ACFM8U_000372"
     CDS             complement(13846..14529)
                     /locus_tag="ACFM8U_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6333672.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14717..16051)
                     /gene="glmM"
                     /locus_tag="ACFM8U_000373"
     CDS             complement(14717..16051)
                     /gene="glmM"
                     /locus_tag="ACFM8U_000373"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC6333673.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEVELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWAREGAERIASAIGSL"
     gene            complement(16181..17038)
                     /gene="folP"
                     /locus_tag="ACFM8U_000374"
     CDS             complement(16181..17038)
                     /gene="folP"
                     /locus_tag="ACFM8U_000374"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC6333674.1"
                     /translation="MVGCVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRAGADYVSPEEEWARVEPVLAEVVGWGVPISLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     DVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKTLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            17314..19428
                     /locus_tag="ACFM8U_000375"
     CDS             17314..19428
                     /locus_tag="ACFM8U_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC6333675.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAIGLHASVYRIFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGSNRTAVSIKETKIGLSWK
                     NLWSDQIQVEKWVVSGAELALTRDRNGTWNIQDLFDGAKHSVSVNRIIVENSTVRLDF
                     LQERFTLKEVRLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSNGIGPPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(19493..20971)
                     /gene="ubiD"
                     /locus_tag="ACFM8U_000376"
     CDS             complement(19493..20971)
                     /gene="ubiD"
                     /locus_tag="ACFM8U_000376"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC6333676.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMVPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFHKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGLRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPMFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(20991..21506)
                     /locus_tag="ACFM8U_000377"
     CDS             complement(20991..21506)
                     /locus_tag="ACFM8U_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC6333677.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSRFYDNLENAVYF
                     GMICNQLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGGEHPLVRDFYHGIEYAEAQAR
                     IGAVSELDCSG"
     gene            complement(21513..21947)
                     /locus_tag="ACFM8U_000378"
     CDS             complement(21513..21947)
                     /locus_tag="ACFM8U_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333678.1"
                     /translation="MCGLWLLRALTAKLPQQRYAALFITLFFAAVLVFGRHDSMYWYP
                     VLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEKAVRYTRRVTQVWCVFFIINGTL
                     TALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            complement(22228..22311)
                     /locus_tag="ACFM8U_000379"
                     /pseudo
     CDS             complement(22228..22311)
                     /locus_tag="ACFM8U_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            complement(22314..22814)
                     /locus_tag="ACFM8U_000380"
                     /pseudo
     CDS             complement(22314..22814)
                     /locus_tag="ACFM8U_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            22870..23121
                     /locus_tag="ACFM8U_000381"
                     /pseudo
     CDS             22870..23121
                     /locus_tag="ACFM8U_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         5276 a         6549 c         6038 g         5258 t
ORIGIN      
        1 tcctgatttt tgttaattca ctataaattc ccataaaaaa acggagcaga tacctgcccc
       61 gtttttattt aatccgaaat tttaatctaa atttaaaatt ttgcaccgga ttggtttgcc
      121 atatagtcaa cagccgcttt gacttcgtca tcgctcaaac cggcattgcc gcctttggcc
      181 ggcatcgcgt taaagccttc aagggcgtgt ttgtgcaagg tttctttgcc ttttttgata
      241 cgcggtgccc aatcgtcttt tttgcctatg ccgggaatac cgggaatcga accgccgtgg
      301 cacacctgac aggttgcttc gaagactttt ttaccgtcaa cgccgaccgc aggggctgcc
      361 gcacccttgt cttctgcctt cgcttctgcc ggagctgcac catcggcagg agcagaagct
      421 gttcctgaag cggcattgtc ggcaggcgca gcctcatcag gattcgggaa agaaccgccg
      481 cttttgttcg ccatgtaagt aatcgcccgt ttgagttcct gatcggtcag gtctgccgca
      541 ccgcctttgg caggcatggc gttaaagccg ttcagcgcgt gttggaacaa ggtatcgaag
      601 ccttgcgcga tacgcggtgc ccaatcgccg ttgtgttcca gtttcggagc gttcggcaca
      661 ttgctgtccg ccgcgtggca ttggatacag attttgccga aaatctgttc gccttggcgt
      721 tcgccgacgg ggatgccgtc gcccatcgtc aattgtccga caggctggat acgggtctgc
      781 gttgctgctt ccgtagtggc atcgacatcg ccgaacgagc cgctgcccgc cagcttaatc
      841 aggaaataaa ggactgcaat aacaataacg ataccgctca caagggtaaa cagtgcagag
      901 ccttgggctt tgttgtcgcg gagttgtttc atttggtagg cctcgccgtc aggttaggtt
      961 gtgctgtaaa ttatagtttg gtgtgttaaa cgcagttaac aatattttgc tggattatac
     1021 tgaattcaca gggtctttcc aatcgctatc attgaaaata tgaaaaaatt tgccaacggt
     1081 atctgtataa aacaaataat cctttgaaaa taatggttta tcctcaagaa aactctcctt
     1141 atgccgccat acgccgcctg ccggcgcaag ataacctttg ccaatttgca gaatttacgt
     1201 taaccttacg ttttacgcac ccatagctca gttggaagag tgtcagtttc cgaagctgga
     1261 ggtcacaggt tcgatccctg ttgggtgcgc caattataaa gaggccgtct gaaagataaa
     1321 tatttttcag acggtctttt gacttacttc aaactcttat ttcaagactt ccgcaaatgc
     1381 gcgggcaaca tagtcggtat tcgacgtatt cagtccggcg acgcacatcc tgccggaatc
     1441 cagcaggtaa acggcaaatt cgtcgcgcag cctgcggact tgttccacgc tcaatcctgt
     1501 gtagccgaac atgccgcgct gtttgatgaa ataagtgaaa tcgcgatcgg ggatttgcgc
     1561 agttaagaca tcataaagtt tctgccgcat cgcacggatg cggtcgcgca tcatataaac
     1621 ctcgttttgc cacaaggcgt aaagttcggg gctgttcatc acgtcggcgg cgatatacgc
     1681 gccgtgcgcg ggcgggctgg agtagatgcg gcggacggtg aatttgagct gtccgaacac
     1741 caaatccgct tcttccttat tcgggcaaac cacgcttaag ccgccgacgc gctcgccgta
     1801 gagcgacagg ttttttgaga aggaattgct gacgaacaag ggcaattcca tttccaccgc
     1861 tttgcggacg gcgtaggcat cgctgtccaa atcgccgccg aagccttggt aggcaatgtc
     1921 cataaacgga atcagtttgc gcgttttgat gatgtgcaac acttcgtccc attgttgttc
     1981 cgacatatcc acgccggtcg ggttgtggca gcagggatgg aggatcagga cgctgttttc
     2041 gggcagggtg ttgaaaaacg cggtcatttc gtcgaatttc acgccaacag tggcagggtc
     2101 gtaatatggg taagtgccga cctcgaaacc tgcgccttca aaaatgccgc gatggttgtc
     2161 ccaagtcggg tcgctgacgt aggcgcgcgc ttcgggaaac cagcggtgca ggaagtccgc
     2221 cccgactttg agtgcgcccg agccgcccaa ggtttgtacg gtaacgatgc gcccttgcgc
     2281 aagcgcggga ttgtctttgc cgaacaacaa atgctgcacc gcgctgcggt aagtgtccaa
     2341 gccttccatc ggcaggtagg gcgacggcgc aggcgcggcg gcacgggcag tttcggcgcg
     2401 gctcacggat tccaacacgg gcattttgcc ttcgtcgtcg aaataaatgc ctatgctcaa
     2461 attgactttt tcggggcgcg ggtcgttttt gaaggtttcg accaaactca aaatcgggtc
     2521 gccgggatag tattcgatgt gtcggtacat agtccttacc ttttactttt tcaaaggatt
     2581 ttctttttca acaatacacc actttcgata tggtgcgtaa acgggaattg gtcgaacagg
     2641 gcggcgcgtt cgaccgcatg ggtttccacc aaggtgtcca aattggcacg cagcgtttcg
     2701 ggattgcagg aaatgtagat gatgttgtca aactgcgaca ccagcttcaa agtttcctca
     2761 tcgataccgg cacgcggcgg atcgacgaaa atagtggaaa atgcgtaatc cgtcaaagca
     2821 ataccgccat ccttaaggcg tttaaactca cgttttccgg tataggcttc ggtaaattct
     2881 tcagcagaca gacgggcgat tttgatgttg ccgatgcggt tggcttcgat attccattgc
     2941 gccgcgctga tggaggtttt ggagatttcg gttgccaaaa cacgttcaaa cttttcagat
     3001 aaaggaagcg taaagttgcc gtttccgcaa tacagttcga gcaaatcccc atccaatcct
     3061 tctgctgcac cgcaggccca ttcaagcatt ttctgacaca cggcggcatt cggttgggta
     3121 aaactgcctt caatttgccg ataacggaaa tcccggttgc cgaccttcaa agtttccgtt
     3181 acatagtcct gttttaagac tattttctgt cccctgctcc gcccaataac ggaaatatcc
     3241 aactgttgct gtaacgcttg cgccgcctgc atccactcat catcaagcct tttgtggtaa
     3301 atcatggtaa ccagcatttc cccgctgagc gtagacaaaa attcgacggc ataccaacgg
     3361 tttctcagtt cggcagattg ggcggcaacg gcaattaatt cgggcatcag gcagttgact
     3421 gcctctgaag cagcatcgaa gcggtcgcaa cggatcaggc ttgcgccgct ggctttctgc
     3481 cctttttcaa acatggcata aaacatttcc ccgccttcgt gccaaatacg gaactcggca
     3541 cgcatacggt agtgtttgtc cggagattcg tacacttccc actcaggaac atccaaacct
     3601 gcaaaaaggg ttttaaggtg gtctttttta tcttgaagct gctgcgtgta gtcgctcata
     3661 tcgctatcct gaaaaatcag accggattat aagccgattc actgcaaaac acgcaacccc
     3721 tttaccggca tataacccgt tttcctccaa cttttatgct aaaatccgcc gtctccaaca
     3781 taccgccgac agaaggaaaa tatggcaggc aacactttcg gacaactctt caccgtgacc
     3841 acgttcggcg aaagccacgg cgcaggtttg ggctgcatca tcgacggctg cccgcccggc
     3901 ttggaattaa gcgaagcgga tatccaattt gacctcgacc ggcgcaaacc cggcaccagc
     3961 cgccacgtta cccaacgccg cgaagccgac caagtcgaaa tcctctccgg cgtattcgaa
     4021 ggcaaaacca ccggcacacc catcgccctc ttaatccgca ataccgacca gcgcagcaaa
     4081 gactatggca acatcgccac cagcttccgc cccggccacg ccgactatac ctattggcac
     4141 aaatacggca cgcgcgacta ccggggcggc ggcaggagtt ccgcccgcga aaccgccgcc
     4201 cgcgttgctg ccggagccgt tgccaaaaaa tggttgaaag aaaaattcgg cacggaaatc
     4261 accgcctacg ttacccaagt cggcgaaaaa gaaatccggt ttgaaggctg cgaacacatt
     4321 tcccaaaatc ctttttttgc cgccaaccat agccaaattg ccgagctgga aaactatatg
     4381 gacggcgtgc gcaaatcctt ggattccgtc ggcgcgaagc tgcatattga agccgccaat
     4441 gtccctgtcg gcttgggcga acctgttttt gaccgcctcg atgccgaaat cgcctacgcg
     4501 atgatgggca tcaacgccgt caaaggcgtg gaaatcggcg caggttttga cagcgtaacg
     4561 caacgcggca gcgaacacgg cgacgaactg accccgcaag gcttcctgtc caaccattcg
     4621 ggcggcatcc tcggcggcat cagcaccggg caagacatct gcgtcaatat cgccatcaaa
     4681 cccaccagct ccatcgccac cccgcgccgc agtatcgaca tcaacggcaa ccccatcgaa
     4741 ctcgccacgc acggcaggca cgacccctgc gtcggcttgc gcgccgcgcc gattgccgaa
     4801 gctatgctcg ccttggtttt aatcgaccac gccctgcgcc atcgtgcgca aaatgcggat
     4861 gttgccgtgg atacgcccga tatcgcccgc tcggacaagt aaaaagattt agccaaaaca
     4921 cgggctttat actaaaatac cgaccattgc cgtgccgtct gaacgcacga ttcttagagg
     4981 aatacaaccg aaatgacaca agaaaccgct ttgggtgcgg cactgaaatc cgccgtccaa
     5041 actatgagca aaaagaaaca gaccgaaatg atcgccgacc acatctacgg caaatacgat
     5101 gtgttcaaac gcttcaaacc gttggcactc ggcatcgatc aggatttgat tgccgctttg
     5161 ccgcaatacg atgccgcact gattgcacgc gtcctcgcca accactgccg ccgtccgcgc
     5221 tatctgaaag ccttggcgcg cggcggcaaa cgtttcgatt tgaacaaccg tttcaaaggc
     5281 gaagttaccc ccgaagaaca ggcgatcgcg caaaaccatc ctttcgtgca gcaggctttg
     5341 caacagcagt ccgcccaagc tgccgccgaa acgccgtctg ttgaagccga agcagcagaa
     5401 tcttccgcag cagaataaat ccccaaatga aatgccgtct gaaaaccgat ttggtttcag
     5461 acggcatttt tccgtatgcg gcaatcacgg ttcaaatatc gagttccgcc gtatcgcctt
     5521 cgcgctccat ccaggcacgg cgggcggcgg cttcgccttt gcccatcagt ttgacgaaga
     5581 tgtcgcgcgt ctcgtcatcc gcgccttcgg ggatttgtac ctgcaacagg cggcgggtgt
     5641 cggggtgcat ggtagtatct ttgagctggt ctgggttcat ctcacccaaa cctttgaaac
     5701 ggctgatgga ataagcagtt tctttaacgc cttctttttg cagccgctct aaaatgccgt
     5761 caagttcgtt ttggtcgagg gcgtagaatt tgcgggcggg tttgctttta ccttgtgcgt
     5821 tgacatctac gcggaacagc ggcggctggg cgacgtagat gtgtccgtca gcgaccagtt
     5881 tcgggaagtg gcggtagaac agggtcagca gcaaaacttg aatatgcgag ccgtccacgt
     5941 cggcatcgga caggatggcg attttgccgt aacgcaggcc gcttaaatcg ggatggtcgt
     6001 tgatgccgtg cggatcgacg ccgatggcaa ctgaaatgtc gtggatttcg gcgttgccga
     6061 agagctggtc ggggtggact tcaaagctgt tgagcacttt gccgcgcagg ggcaggatgg
     6121 cctgggtggc tttgtcgcgg gcgagtttgg ctgagccgcc ggcggaatcg ccttcgacaa
     6181 ggaagagttc gttttcgcgg atgtcttcgc tttcgcagtc ggtcagcttg ccgggcagga
     6241 cggcgacgcc gctgcctttt ttcttttcga ttttcttaac cgaacgcatc cgcgcctgcg
     6301 cctgacggat ggcgagttcg gcaatttttt taccgaagtc cacgttttgg ttcagccaca
     6361 attccaaagg gtcgcccgct acagcggcga caagtttcag cgcgtcgcgg ttggtcagct
     6421 tgtctttggt ctgaccttgg aactgcgggt cgaggacacg ggcagagagg acaaaggcgg
     6481 ttttgccgaa cacgtcgtcg ctttgtactt tcacgccgcg cggcaagagg ttgtgcagat
     6541 tgatgaaatt gttgacggcg ttgaacacgg cttgtttcaa gcctgcttca tgcgtgccgc
     6601 ccaaaggggt gggaatcagg ttgacgtagc tttcgttggc acacgagcct tcttccagcc
     6661 aagtcagggc aaacgccgct ccttcgccga tgctgaaatc gccgttgtgt tcgtctgaaa
     6721 tgtagttttc gcaagagaag ataggcacgg cttcctgcgc gtcggcaatc aggtcggtca
     6781 gatagctttt caggccgtcg gggtagtgcc aagttcgggt atgcgcttcg tcttcgcctt
     6841 tgaccggacg ggtcagggaa acgcgcacac ccggcagcag cacggctttg gcacgcagca
     6901 ggcgttcgag ttcgggaatg ctgtaattcg ggctttcaaa atatttgccg tccggccaca
     6961 cgcgcacttc ggtgccgctg tctttgacgg cgcatttgcc cacttgtgcc aacggttcga
     7021 ccacgtcgcc gccggcaaac acgatacggt ggattttgcc ttcgcgtttg accgttacct
     7081 caaggcgggt ggaaagggcg ttggtcacgg atacgcccac gccgtgcagg ccgcctgaaa
     7141 aggcatacgc gctgccgccg tcttttttat tgaacttgcc gcctgcgtgc agacgagtga
     7201 acacgagttc gaccacgggt acgccttctt cagggtgcag gccgacggga ataccgcggc
     7261 cgttgtcgcg tacggaaagc gaaccgtctt catgaatttg cacgtcgatt gccgtagcga
     7321 aaccgcccaa cgcctcgtcc gccgcgttgt cgatgacttc ttggcagata tgggtcgggc
     7381 tgtcggtgcg ggtgtacata ccgggacgtt ctttgaccgg ctccaagcct ttgaggacgg
     7441 tgatgctgga ttcgctgtat tggttgtttt tagccatagg aataatctga aagtaagaaa
     7501 aacaacgctt tcagacggcc tgaaagcgtt gcgttccgtt gttttagcgg ttgtcggaag
     7561 attggcgggc ggcaaagtct tcataacttt ccataccgcg caggaagcgg gaagagagtt
     7621 ctttcaacgc ccccaaataa acgtcgcgtt tgaaatcaat cacttcgtca acgggcgccc
     7681 aatattgatg ccaacgccag ccgtcaaatt cggggtggcg ggtggcgcgc aggttgacat
     7741 cgcaatctcg gccggtcagg cgcaggagat accaaatctg cttctgtccg cgataagagc
     7801 cgcgccattc gcggcgtacc cagttgttcg gcacgtcata acgcagccag tcgcgcgtgc
     7861 ggccgataat tttgacgtgt tgcggcaaaa gcccgacttc ttcgtacaac tcgcggtaca
     7921 tggcggtttc ggggctttcg cccggcttga tgccgccttg cggaaactgc caagaatgtt
     7981 cgcgcacgcg cttaccccaa aagacttcgt tgcggttgtt gattaagatg ataccgacat
     8041 tggggcgata gccttccctg tccaacacgg tgtcgccctc cgttaaattc aatcttggga
     8101 ttttcccaca aatcaggcgg ttttgacaaa tcagacggca tggcggtacg cgtgccgaaa
     8161 cacgggggga tttgggaaaa tatcttaaat ttggtttaca ataatgtatt tcaaattatt
     8221 cgggaatcag accatgttag atatccaatt gctccgcagc aacaccgccg ccgttgccga
     8281 acggcttgca cggcgcggtt atgactttga taccgcacgt tttgacacac tggaagaacg
     8341 acgcaagtcc gttcaggtga aaaccgaaga attacaggcc tcgcgcaaca gcatttccaa
     8401 acaaatcggc gcactgaaag gtcagggcaa acacgaagaa gcgcaggcgg ccatgaatca
     8461 ggttgcccaa atcaaaaccg atttggaaca ggctgccgcc gatttggatg ccgttcaaaa
     8521 agaattggac gcatggttgt tgagcatacc caacctgcca catgaggacg tgcctttcgg
     8581 taaggatgaa accgaaaacg tcgaagtccg caaagtcggc acgccacgcg aatttgactt
     8641 tgaaatcaaa gaccatgtcg atttgggcga acctttgggt ttggatttcg aaggtggcgc
     8701 gaaactttcc ggcgcacgat ttaccgtgat gcgcggacaa atcgcccgcc tgcaccgcgc
     8761 gctggcgcaa ttcatgctcg atacgcacac gctgaaacac ggctacaccg agcattacac
     8821 gccttatatc gttgacgata cgacgctgca aggcacgggt cagctgccga agtttgcaga
     8881 agatttgttc cacgttaccc gtggcggcga cgagagcaaa accacgcaat atctgattcc
     8941 gacagctgaa gttaccctga ccaataccgt tgccggcagc attatcccgt ccgaacaact
     9001 gccgctgaag ctgaccgcgc attcgccctg tttccgcagc gaggcgggtt cgtacggcaa
     9061 agacacgcgc ggtctgattc gccagcacca gttcgacaaa gtggaaatgg tgcaaatcgt
     9121 tcatcctgaa aaatcatacg aagcgctgga agaaatggtc ggccatgccg aaaacatcct
     9181 gaaggctttg gaactgccct accgcgtgat taccctgtgt accggcgaca tgggcttcgg
     9241 cgcaaccaaa acgtatgatt tggaagtttg ggtaccggcg caaaatacct accgcgaaat
     9301 ctcaagctgt tccaactgcg aagatttcca agcccgccgc ctgaaggcgc gtttcaaaga
     9361 cgaaaacggc aaaaaccgct tggtacatac tttgaacggc tccggcttgg ctgtcggcag
     9421 aacgctggtc gccgtattgg aaaaccatca aaacgccgac ggcagcatca acatccctgc
     9481 cgcactgcaa ccgtatatgg gcggtgttgc caagttggaa gtcaaataag tttgcaggct
     9541 gcctgaacgt caaatgccgt ctgaaacctg tttcagacgg catttccttt aaacttttaa
     9601 aacacgtcag ccgtcggcac gaaccgcatt gccgcaatcg ccggtctgtc cgacctcgcg
     9661 gatattggac agcgtaactt ccgaaatatt acccaacgcc tcttccgtca aaaatgcctg
     9721 atggccggta aacagcacat tatgacaaga cgacaggcgg cggaacacgt cgtcggtaat
     9781 cacatcgttg gatttgtctt caaaaaacag ctcgcgctcg ttctcgtaca catccatgcc
     9841 caatgcgccg attttccggc gtttcaacgc ctcaatcgcg gcggcactgt caatcagccc
     9901 gccccggctg gtgttgataa tcatcacgcc gtctttcatt ttgtcgaacg ccgcttcgtt
     9961 cagcatatag tggttttccg gcgtggcggg gcaatgcagc gtgatgatgt ccgaccgggc
    10021 atacagctcg tctaaatcca catatttgcc gccgattttt tccgcttcgg ggttgcaaaa
    10081 cggatcgtaa gccagcaggt tcatgccgaa accctttaaa atccgcatgg ttgcaatacc
    10141 gattttcccc gtgccgataa cgcccgccgt tttgccgtac atattgaaac cggtcagacc
    10201 ttccagcgaa aaattcgcat cgcgggtacg ctgataggct ttgtggatac ggcggttcaa
    10261 cgtcagcatc agaccgaccg tatgttccgc aaccgattcg ggcgaatagg caggcacgcg
    10321 cacaactttc aagcccaact cttcagccgc ctttaaatcc acattattga agccggcaca
    10381 acgcaacgcc acagttttca cgccaatttg cgccaatttt tccaacacgg gccggctgcc
    10441 gtcgtcgttt acaaaaatac agaccgcctc cgcaccttcc gccattttcg ccgttttcac
    10501 atccagcata aaatcaaaaa actccagctc gaagccgaaa tgccggttgg cgcgggtaaa
    10561 atgttcgcgg tcataacttt tcgtaccgta aatcgcaatc ttcatcaata tgtccagttg
    10621 tcgcctatgg ttgagaaacg gcataataca ctgaattcaa acaaatcagt agaatatggc
    10681 agattaaaat tgattgcatg cacggcattt ccatttcaaa acacaaaact caatcgccca
    10741 ttgccgccag aagctccgcc tgatgctcgg caatcagggc atcggtgatt tctgccaaat
    10801 cgccgtccat cacaaaatcc agcttgtgca gggtgaggtt gatgcggtgg tctgttaccc
    10861 tgccttgggg atagttgtag gtgcggatgc gttcgctgcg gtcgccgctg ccgatgaggg
    10921 atttgcgttc ggcggcttct ttggcttggg cttcgcgttt ttgcgcgtcg ttcaggcggg
    10981 cggcgaggac tttcattgcc tgcgctttgt tggcgtgttg gctgcggccg tcttggcatt
    11041 cgaccaccat accggtgggc aggtgggtga tgcggacggc ggaatcagtt ttgttgatgt
    11101 gctgaccgcc cgcaccggat gcgcggaagg tgtcgatgcg cagatcggcg gggttcaact
    11161 cgatgtcttc cagttcgtcc gcttcgggca tgacggcaac ggtgcaggcg gaggtgtgga
    11221 tgcggccttg gctttcggtg gcggggacgc gctgcacgcg gtgtccgccc gattcaaatt
    11281 tcaaacggct gtacgcgcct aagccgataa tacgggcgat aacttcttta tagccgccca
    11341 actcgctttc gttggcggac acgatttcaa cctgccagcg gttgcgttcg gcgtagcggc
    11401 tgtacatacg cagcaaatcg ccggcaaaca gcgcggcttc gtcgccgccg gtaccggcgc
    11461 gtacttcgat aaagatgttt ttgtcgtcgt cggcatcttt gggcagcagc aatttttgca
    11521 gttcggtatc gagcgcaccg attttggctt tcgccgcttc gatttcttcg gcggcaaagt
    11581 ctttcatttc ggggtcggac aacatttctt cggcatccgc caagtcgctt tgggcaagcc
    11641 gatagttttg gaacacttcg acgacggggg tcagttcagc gtgttcgcgc gtgagcttgc
    11701 ggtagttgtc catatcggac gtggcttcgg gttgccctaa aaggtgggta acttcttcca
    11761 accggtcgcc gagttgttgc agtttttcta agatagacgg cttcataatt cttccataac
    11821 aaacgccgcc tgaagttcag acggcattaa aacgcggtca ttataatata ttttctgaat
    11881 attcaaaaag ataatctagg gacggagcag gctgtcggca ttgcgttccg ttatcgcggc
    11941 gatttccgcc gcgccggtac cgcgtatctg cgcggcaatt tcggcaatcc gaagaatatt
    12001 ggcaggcgta ttgacggtgt ttttcaacat gaacgggcta tccgtttcca atacgaaatc
    12061 cccgtcgttc aaggctttaa gcgtatcgcg cactttacgc gcgttcgggt tgagcagcag
    12121 cgaaccgata ccgattttga aaccgagccc gaccaacacc cgcgcttctt caatgctgcc
    12181 ggaaaaggcg tggacgatgc cgccacagga aaaacctgtc tgtctgacgg cggcggcaat
    12241 ctcgttcgag gctttcaggt tatggataat cacgcggcgg cgcagggttt gcgcgatggc
    12301 aagctggcgg ctgaaaactt ggatttgccg ttcgcgctgc tgcggcgttt gggttttatc
    12361 gtaaaaatcc aagccgattt cgccgaccca tgccatcgga cgcgctttca atgcttgttc
    12421 caaacgctgg aaatccggtt cggaaacgtc gtctgaaaac caaggatgga tgccgagcgc
    12481 gatatggata ttgccccaaa gcggattttc agacaacctt tgagccaaat ccgccacgtc
    12541 ctgccaatcc tgcggccgag tcgcgggaac gataaaccgc cctacccctg cctcccgcgc
    12601 ggcggtcagg atgtgcggca ggttttcgcg cagggcggca tcggcaaggt ggcagtgggt
    12661 gtcggtgaag ttcatttcga ttccgcaatg cggcaggttc acataaaaat aaggcagggc
    12721 ggcaagggat gccgtgtacg ggcggcgcat aaggcgcggt caacgccccg tcattgccat
    12781 ttcaatccgc tataacttca gtcttgccaa ctctttttcc acctcttcct taccccagcc
    12841 ttgcgatacg gcgagggtca tgagtgcggt ggcggtttcg aggttgcatt tgccgccgtt
    12901 gatgatgccc gagttgcgga acgcattgcc ttgcgcgtaa acggcggcgg ttttgccttg
    12961 tcggacttgg ctgatgttga gcaacaattt tccttgccgc gtgaagtctc ggacagcgcg
    13021 gataaagcct tcgtctgcgg gggtgttgcc gtgtccgtag ctttgcagga taagggcttg
    13081 ggcggaaagc cgtccgagtc cgtccgcaag ttcttggacg gcaaagccag ggataagcgt
    13141 gcggacggcg attttcgcct gcgggtcggg ataatggatt ttgaggccgt ctgaaacatc
    13201 tgccgcgtcc tgattcggga tgcggacatt gtgccagccc tgggtttcgt cccactccgc
    13261 cagtgcgccg aaatgcggat tgtcgaaacc ggcggcggtt tcggtgctga ctttgctgct
    13321 gccgacggcg ggatacagtt tgccgtcaaa ggcaataagc gtctgcttca atccgagtgg
    13381 gaatgcggca acggcagtcg ccagattgcg cggcgcgtcg ctgcccacgg cgttgtacgg
    13441 ccattgggag ccggtcagga cgattggctt gtccaaaccc tgcagggcga gggcgaacag
    13501 attggcggta taggcgagcg tgtccgtgcc gtgcagcacc aaaacgccgt cgtactgcgg
    13561 cagcgcggcg gcaatgatgt ccagccaagc gcaccagttt tgcagttgga cggcggaaga
    13621 atcaatcagc ggcccacaaa cgtgccaatc gaaatccatg ccgtttgaaa acggcgcgag
    13681 tgcctgcccg accagcgcgg tatcggggcg caggccttcg ctgctttgcg tcatgccgat
    13741 ggtgccgccg gtataaagga caaaaatttt ctgtttcatg gctcatcctg aaaatgcaaa
    13801 gaccgcctga aacgcaaaat gcgtccggac ggtcgaaaat ctgatttact gctgcttctg
    13861 tgccgctttt ttcgctgcct tttccgcctt gcgtttggcg cgtttttcgc tcaattgtgc
    13921 gcggtaaagc tgataatgtc ggcgtttgcg ccaccagaac caagccagcg ccgccgccag
    13981 cacgcccaat gcgatgaaga tgccggattg caggctgtgc attttcgcca tcagccaatc
    14041 gatgttgtgc gcgccgtact cgcccaagta aatccaaacg ggcacggaaa tcagcgcggc
    14101 aagcccgtcc ataatcagaa agcgcagata cgatactttg cggctgatgc cggcggtaac
    14161 gaaaacggca gtccgcaaac cgggcaggaa acgagcgaca aataacaccc agttgccgta
    14221 tttgtcgaat ttttcctgaa cctgtgcgta acgtttcggc gtcatgatgc gcgcaatcgg
    14281 tttgaacttg aggattttct gcccccaaat tcgtccggcg gcgaacatga tgccgtcgcc
    14341 cgccaacacg ccgagcatgc cgactgcaaa cataatatgc ggattggtat aacccatacc
    14401 cgaaatcacg ccgcctgtta ccaaggtcaa atcctcggga atcggcacgc cgaaaccgca
    14461 gatgaccaaa acgaaaaaca cggccgcgta gccgtattcg acaaaaaagg cttccaaaag
    14521 ggcaaacata gcggatattc cattgtcgga gataaaaagt cagaacaagc cgaaacattt
    14581 tctgcgcgaa acggcattct atcaaagatt atgccgtctg aaagcggcag ggggcagatt
    14641 cgcgcccccg ccggacgttc agacggcatt taaacaggaa aaccgccgct gttgaaacgg
    14701 tttttcctga ttttgcctaa aggctgccga tggcggaagc aatacgttcc gccccttcac
    14761 gcgcccaatc ggcctgtcgt gcctcaacca tcacgcgcac gacgggttcg gttcccgaag
    14821 cgcgcaatac aacccttcct ttgccttcga gttctttttc cacttcggcc aacacgtctt
    14881 tcgaagcttc ctgccatttc tgaccttttt ggatgcgcac gttaatcatg gtttgcggat
    14941 acggctgcca gtcggcgcaa acagtggcaa ggtcttggtt caggatttgc agcgccgcca
    15001 aaacttgcag cgcggaaata atgccgtcgc cggtgttgtg tttgtccatg cacaaaatat
    15061 gaccgctggc ttcgccgccg atgagccagc cgcgttggtt cagctgttcc aacacatagc
    15121 ggtcgccgac tttggcgcgg cagaaatcga caccctgctc tttcagggcg atttccatcg
    15181 ccatattggt catgaccgtg ccgaccacgc cgccgatgtt gataccttct cgggcgcggg
    15241 ctttggcaat gacgtaaatc aggctgtcgc cgtcgtaaac cttaccattt ttatcgacca
    15301 tcatcagtcg gtcgccgtcg ccgtctaagg cgatgccgta gtcggcttca tgctgtaaaa
    15361 cggcggcctg gagtgtcttg gtataagtcg caccgcattt ttcattgata ttgtagccgt
    15421 taggttcgtc gccgatgctg acgacctgtg cacccagttc gtgaaacacc ttgggtgcga
    15481 caccgtaccc cgcgccgttg gcggtatcga taaccaactt caaaccccga aggtcggaat
    15541 ggctggggaa ggtggatttg caaaattcga tatagcggtc gtccgcaccg ctgatgcggc
    15601 gtgcgcgccc gaggcgggcg gaaggctgtg ttttcatttc ttcatcgatt ttggcttcga
    15661 tttccaactc gacttcatcg ctcaatttca cgccgccctc ggcaaagaat ttgatgccgt
    15721 tgtcggaata ggcattgtgc gacgcggaaa tcatcacacc ggcggacaaa cgcagcgcac
    15781 gggtcagata agccacgccg ggcgtaggca gcggaccggt ctgtaccaca ttcacacccg
    15841 ccgccgtaaa accggccacc aaagcggctt ccagcatata gccggaaatg cgcgtgtctt
    15901 tgccgatgag gacggtcggt ttctggtcgg tgtcgtgctg caccaaaacc tgccccgccg
    15961 catagccgag tttcaatacg aaatcgggcg taatcggaaa ttgccccact tcgccgcgca
    16021 cgccgtccgt gccgaaatat ttttttgcca tgtgttgctc cgagaatgtg aaccgttgtc
    16081 cgagattata cagtcagttt gtgccttgct gtctgcaccg ttgatgccgt ctgaagccgc
    16141 cccgtccttt tcagacggca tgaagtatgc gaaccgccgt ttacagattg atgcccaacg
    16201 cttcccacac cttcaacgca tccgctgtcg ccttcacatc atgcacccgc acgatttgcg
    16261 cgccgcgcgc tacggaagcc aacgctgccg ccacgctgcc gtgtacgcgt tccgccgcat
    16321 ttgcctcgcc ggtcagctcg cctatcgtgc ttttgcgcga tacgccgatg agcagcggaa
    16381 aacctgtttc cgccatcaat tcgggcaaat gccgcatcag cgcgatattg tgttgcaagg
    16441 ttttgccgaa gccggagccg aagccggggt cgagtatgat gcgttgcggt gcgatgcctg
    16501 ccgcgataca ttccgctgag cgcgctttca aataccgcgc tacttcaccg acaacatctt
    16561 gatatttcgg attaatctgc atggttttgg gcaaaccctg catgtgcatc aggcaaatgc
    16621 ccgtgtccgc ctgacgcgcc agcaattcga ccgcgccttc gtcggtcaac gccgccacat
    16681 cgttaataat atcgatgccg ccgagtgcca atgctttttc cataaccacc gtgcggcgcg
    16741 tgtccaaact gatgggaacg ccccatccaa ccacttccgc caatacaggc tcaacccgcg
    16801 cccattcttc ttcaggcgaa acataatccg cacccgcccg cgtcgattcg ccgccgatgt
    16861 cgagaatgtc cgcaccttct ttcaaaagcc gttcggcatg cgccaaagct gtttgggcgt
    16921 tttgcgaata cgcgccgccg tcggaaaaag aatcgggcgt gagattcacg atacccatga
    16981 ttttcggctt gtccaaaccg atttcaaacc gtcctgcctg ccaaacacat ccgaccatat
    17041 ccgtgcctct ggaataaaaa gcaaattata taccgtctga acaaaacttg tccgtttcag
    17101 acagcatcgg tatttcaaaa aagtatcttg cagaatccgc ctccgtatga aacatacact
    17161 gcccctgcaa acgcggcaac gccctccata cccgattcgg cggtatgcct gccgacgggc
    17221 attgcccctt taatgcaaga taatccgctc gcataaaata agattgccgc aagccgtgtt
    17281 atactgtggc gtgttttacg ggcttttcag gctatggatt tattatcggt tttccacaaa
    17341 taccgtctga aatatgcggt agccgtgctg acgatactgc ttttggcggc aatcgggctg
    17401 cacgcttccg tatatcgcat cttcacacct gaaaacatcc gaagccgcct ccaacaaagc
    17461 attgctcata cgcaccggaa aatctcgttt gatgcggata tacagcgcag gctcctgccc
    17521 cggccgaccg tcatcctgaa aaacctgacc attaccgaac ccggcagcaa ccggactgcc
    17581 gtttccatca aagaaaccaa aatcggattg agctggaaaa atctgtggtc ggatcagata
    17641 caggttgaaa aatgggtggt ttcgggtgcg gaacttgccc tgacgcgcga cagaaacggc
    17701 acttggaaca tccaagatct gttcgacggc gcgaaacact ccgtctcagt caaccgcatt
    17761 atcgtcgaaa acagcaccgt ccgcctcgac ttcctgcaag agcggtttac cctgaaggaa
    17821 gtccgcctca acctgcaatc ccccgattcg tcggggcagc cgtttgaaag ttcgggcata
    17881 ctggtttggg gaaagctgtc cgtcccgtgg aaaagcaggg ggctgttcct ttcaaacggc
    17941 atcggcccgc ccgaaatctc accgttccat tttgaagctt ccacttcgct ggacggacac
    18001 ggcattacca tttccaccac cggcagccct tctgtccgct tcaacgccgg cggagcggat
    18061 gccgccggcc tcggcctgcg tgcagacact tccttccgta acctccacct gaccgcccaa
    18121 atccctacgc tggcactcag gaacaacagc attaaaattg aaaccgtcaa cggcgcattt
    18181 accgccggcg gcgaatatgc ccaatgggac ggttcgttca aactcgacaa agccaacctg
    18241 cactccggca tcgccaacat cggcaacgcc gaaatctccg gcagcttcaa aacaccgcgc
    18301 caccagacca acttctccct caattcgccg ctcgtatgga cggaaaacaa agggctggac
    18361 gcgccgcgcc tgtatgtatc gacccttcag gataccgtca accgcctgcc gcaaccccgt
    18421 ttcatcagcc ggctcgacgg ttcgctgtcc gtaccgaatc tgcaaaattg gaatgccgaa
    18481 ttaaacggca cattcgaccg ccaaaccgtt gccgcgaaat tcagatacac acatgaagac
    18541 gcaccgcatc tggaagccgc cgtcgcactg caaaaattga acctgacccc ctatcttgac
    18601 gacgtgcggc aacaaaacgg caaaatattt cccgacaccc tcgccaagct gtccggcgac
    18661 atcgaggcgc acctgaaaat cggaaaagtc caacttcccg gcctgcaact ggacgatatg
    18721 gaaacctacc tccacgccga caaagaccat atcgcgctca gccgtttcaa gtcagggctt
    18781 tacggcggcc ataccgaagg cggcatcagc atcgccaaca cccgtcccgc cacttaccgc
    18841 ctgcaacaga atgcaagcaa catccaaatc caaccgctgc tgcaagacct gttcggcttc
    18901 cacagcttca gcggcaacgg cgacgcggtc atcgacctga ccgcgggcgg cgaaacccga
    18961 aaagagctta tccgctcgct tcaaggcagc ctgtcgctaa atatttccaa cggtgcgtgg
    19021 cacggtatcg acatggacaa tatcctgaaa aacggcattt cgggcaaaac tgccgacaat
    19081 gccgcaccca gcacaccctt ccaccgattc acgctcaaca gcgaaatttc agacggcatc
    19141 agccgccaca tcgataccga actcttctcc gacagcctct atgttaccag caacggctat
    19201 accaatctgg atacgcagga attgtctgaa gatgtcctta tccgcaacgc cgtccatccg
    19261 aaaaacaaac cgattcccct gaaaatcacc ggtacggtgg acaagccgtc cattaccgtc
    19321 gattacggca ggctgaccgg cggcatcaat tcgcgcaaag agaaacagaa aatcctcgaa
    19381 gacaccctgc tggaacaatg gcagtggctc aaacctaaag aaccgtaaac atcctgcgta
    19441 cacaaatgcc gtctgaaaca cccccacgct tcagacggca tgaccgtaaa acctacaacc
    19501 ccaattcctc ccaaatccca tcaatcttag ccgtaaccgc agggtctttt ttgatgacgc
    19561 gtccccattc gcggtcggtt tctcccggcc atttgttggt cgcatccaaa cccattttgc
    19621 cgccgagtcc gctgacgggg ctggcgaagt cgagataatc gatgggcgtg ttttctacca
    19681 aaacagtgtc gcgcacgggg tccatgcgcg tggtgaccgc ccagatgact tctttccagt
    19741 cgcgcacgtt cacatcgtca tccaccacga tgatgaattt ggtatacata aactggcgca
    19801 ggaacgacca gcagcccatc atcacgcgct tggcgtgtcc ggcgtactgt tttttcatgc
    19861 tcaccaccgc catgcggtag gagcagcctt cgggcggcag gtagaaatcg gtgatttcgg
    19921 gaaactgctt ttgcaagagc ggtacgaaca cttcgttcaa tgccacgccc aaaacggcag
    19981 gttcatcggg cggtttgcct gtgtaggtcg aatggtaaat cgggttttcg cgcatggtga
    20041 tgcgttcgac cgtaaacatg gggaaatggt cctgctcgtt gtaatagccg gtgtggtcgc
    20101 cgtatgggcc ttccagcgcg gtttcgttcg gatgaatcac gccttccaac acaatttcgg
    20161 cgcgtgcagg cacttgcaaa tcgttgccga tacatttcac cagctccgtc cgcaaaccgc
    20221 gcagcagtcc ggcaaactgg tattcgctca aggtatcggg aacaggcgtt accgcgccca
    20281 aaatggtggc ggggtcgcag ccgagtacga cggcgacggg atacggcgta tcgggattga
    20341 gtttgtggaa ttcctgataa tccaacgcgc cgccgcgatg cgacagccag cgcataatca
    20401 gcttgttttt gccgatgagt tgttggcggt aaatgccgag attttggcgt tttttgtgcg
    20461 gcccgcgcgt gacggtcaag ccccatgtta ccagcggcgc aacgtcttcc ggccagcaat
    20521 gctgaatcgg aagttgatac aaatcaacgt cttcgccttc ccacacgatt tcctgacacg
    20581 gcgcgttttt caccacgttc ggcaccatgc tccaaatgtc tttcaagagc ggcagtttgg
    20641 aaaacgcgtc tttgatgcct ttgggcggtt cgggttcttt caaatacgcc agcgtctgcc
    20701 cgatttcgcg cagcttggac acgctgtccg cacccatgcc cattgccaca cgttcgggcg
    20761 tgccgaacag gtttgccaac acgggataat cgtagcgcgt accgtcgggc ttaactgggt
    20821 tttcaaacaa caatgccggc ccttcggcgc gcagcacgcg gtcggcgatt tcagtcattt
    20881 ccaaatgcgg ggaaacagga tgcgcgacgc gcttgagttt gccctgctgc tcgagcatag
    20941 cgatgaagtc gcgcaggtct ttgtatttca tatttatcct ttttgtcctt ttatcctgaa
    21001 caatccaatt cggataccgc ccctatcctt gcctgcgctt cggcatattc tatgccgtga
    21061 taaaagtcgc gtaccagcgg atgttcgccg ccttgatgga gttgcaacaa aggacgttga
    21121 ccatcgggtt gggtaacgac attgcagtgc agaccgaagg tgtcggtttc ataagggggc
    21181 agctggttgc agatcatgcc gaaataaaca gcgttttcaa ggttgtcgta aaagcggctt
    21241 tgatagtcgt taaaactctt ttcgctgacg gatacccaca cgccatattc cagcgtttcc
    21301 tcatgcccga taatcggaat cggcagcacc gcgcggataa agcggtcggt ttggtcggga
    21361 tagcggatga tgcagaaatc agaatcgcat tctgcttgat aagcaatgcg ttcttcttca
    21421 ctgagttgat tatagggatc gggggcggta aagccgattg cgggcatatc ttcatgtttt
    21481 tcgccgcagg aagtgcaagt gtacataagg tttcagactt tcaaaacgag tttgcggtaa
    21541 agccattcgc cggcaaacag catccccatc aatacatagg caatcacgcc tgtataaacc
    21601 gcccaccaat catatcgccc caaccgagcc aacaaagcgg taagcgtccc gttgattata
    21661 aaaaatacgc accaaacctg cgttacccgg cgggtatagc gcacggcttt ttcaggcagg
    21721 tcgggctgtt gcagccgcgc aagtttttca atcaccgtct gtccggcaaa caggcttccc
    21781 ccgaacaccg ccaacatcat cagattgacg aggacgggat accaatacat tgaatcatgc
    21841 cgcccgaaca ccaatactgc ggcaaaaaac agtgtaataa acaaagccgc ataacgctgt
    21901 tggggcaatt tggcagtcag ggcgcgcagc agccacaacc cgcacatcgc cgccgccagc
    21961 cagacaaacc agcccgcctc cctgccgtaa tatagtggat taacaaaaac cagtacagcg
    22021 ttgcctcgcc ttgccgtact atctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
    22081 actataccac aaagcgggat aggcaatgct taatacggtc agaaaaatat gtccgaaaaa
    22141 accgggtttc attttgaatc cgcacaaatg ttttcagacg gcatccgata aaaacatgcc
    22201 gtctgaaaaa taattagaaa tacccgatta gcccgcctga atcttcaata ccgcctgtac
    22261 cacgtcgttg acggtgcgga cattgcggaa atcttcagcc tgcagcttgc gggctgccac
    22321 agccattcac gattgcgata tacccggcgg cattcccgac tgccgttcag cagaaagcgc
    22381 acccattcca aaccgctcca atatgcctcg ggcagcatac cggcttcaga cggcatatcg
    22441 tccgaagcag ataaagtcag gctgtaacgc gtccctttgg tcagaaccat cgccaaagca
    22501 taagcaaacg gcgcgcgcgt tgccgatacg gcatatcctt ccggcagcgg atcgtccgcc
    22561 gccaaaacca aaaccgaccc gcatccctct tccaacagtg atgccgcttc cgccaatgcc
    22621 gtttccacac cgtcggcaca cacggccaat gccgtctgct cgctcatatc ccgcctcaat
    22681 atcgaccatt accccgccgt tgcattgtgc accgacaaac cgaacgaagt cggcgacacg
    22741 gtttgcgatt tcaacagttc caaccacaaa tcgaaactgc gcgccatttc cccgtcgtgc
    22801 gaggcataaa ctatagtgga ttaaatttta ggggctgtac tagattagca gatatgttac
    22861 cctcgaaata tgaagataac acactgcaaa ttaaagaaaa aagtacagaa agaactgctc
    22921 cgttttttgt gctggaagtt accgcccgtt ctgccgccga tattttgggt atccatccca
    22981 attcggcagc actgttctac cgtaaaatcc gcacggttat caaccatcat ttggccttgg
    23041 ctgccgatga ggtttttgag ggccctgtcg agccggacga aagcgatttc ggcggacggc
    23101 gtaaaggtag acgtggtcgc g
//
LOCUS       DAXHSJ010000013        22997 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000013 DAXHSJ010000000
VERSION     DAXHSJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22997
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.013"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..181
                     /locus_tag="ACFM8U_000382"
     CDS             <1..181
                     /locus_tag="ACFM8U_000382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333679.1"
                     /translation="QVLDVVSFALGQLRVAHGNLSCRKGRMLPHTGLFLLGKVALQMR
                     IRRSLKFDAPYILRP"
     gene            complement(301..2001)
                     /gene="recJ"
                     /locus_tag="ACFM8U_000383"
     CDS             complement(301..2001)
                     /gene="recJ"
                     /locus_tag="ACFM8U_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC6333680.1"
                     /translation="MSVKIQTRSINTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDAEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTALAVYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLKACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
     gene            complement(2070..2786)
                     /locus_tag="ACFM8U_000384"
     CDS             complement(2070..2786)
                     /locus_tag="ACFM8U_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333681.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDRPADIAKLKQLEFKAVELDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENILHTPPLLILDAA
                     GAVLALPAAALGAVVDAARK"
     gene            complement(2833..3339)
                     /gene="yjgA"
                     /locus_tag="ACFM8U_000385"
     CDS             complement(2833..3339)
                     /gene="yjgA"
                     /locus_tag="ACFM8U_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC6333682.1"
                     /translation="MFEQEDEWISKTQMKKQMNGLQDLGMELTKLSNDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAVHNAFLQRVE
                     QARVRLLADDGALTQFMSDFPHADAGKLRTLVRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            3493..4824
                     /gene="pmbA"
                     /locus_tag="ACFM8U_000386"
     CDS             3493..4824
                     /gene="pmbA"
                     /locus_tag="ACFM8U_000386"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC6333683.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRASTADFSEKALQDTVKAAIDIARYTAEDDCA
                     GLADASLMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFATHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVKGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(5121..5324)
                     /locus_tag="ACFM8U_000387"
     CDS             complement(5121..5324)
                     /locus_tag="ACFM8U_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC6333684.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            5567..5869
                     /gene="clpS"
                     /locus_tag="ACFM8U_000388"
     CDS             5567..5869
                     /gene="clpS"
                     /locus_tag="ACFM8U_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC6333685.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            5871..8168
                     /gene="clpA"
                     /locus_tag="ACFM8U_000389"
     CDS             5871..8168
                     /gene="clpA"
                     /locus_tag="ACFM8U_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC6333686.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDEDDGNYSSDG
                     MDDDNENRTKPGKNPLSEYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPL
                     LVGEAGVGKTALAEGLAHQIVNGDIPDALKEAEVYALDMGSLLAGTKYRGDFEARVKS
                     VLKQLEKIPHTILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEY
                     RTIFDKDHALSRRFQKIDVVEPTVAETVQILRGLKPMFEDFHQVRYTQGALEAAAELS
                     ARYINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSH
                     DDKQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGK
                     TEVAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHC
                     VLLLDEIEKAHPDIFNVLLQVMDAGRLTDNNGKSADFRNVILIMTTNAGAESLSRPSL
                     GFTANRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIAKVVDKFLLQLEHQLLD
                     KKVEAEFTPALRKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRI
                     DWDAAKEEAVLKFKKNKVKPEAEPV"
     gene            complement(8216..11314)
                     /locus_tag="ACFM8U_000390"
                     /pseudo
     CDS             complement(8216..11314)
                     /locus_tag="ACFM8U_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(11345..11454)
                     /locus_tag="ACFM8U_000391"
                     /pseudo
     CDS             complement(11345..11454)
                     /locus_tag="ACFM8U_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11499..12095)
                     /locus_tag="ACFM8U_000392"
     CDS             complement(11499..12095)
                     /locus_tag="ACFM8U_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6333687.1"
                     /translation="MVWKTLGEVFDLKNGYTPSKSNKEYWENGSIPWFRMEDIRENGR
                     ILDNSLKHISKSAVKGGKLFPAKSIMMSTTATIGEHALIKVNYISNQQLTNFTIKDEF
                     KDALDINFAFYYFFIIAEQSKKLINTSSLPIISMKELKKLKIPIPPLPEQEKIAAILD
                     KFDTLTHSISEGLPHEIALRRKQYEYYREQLLAFPKAT"
     gene            complement(12125..12718)
                     /locus_tag="ACFM8U_000393"
     CDS             complement(12125..12718)
                     /locus_tag="ACFM8U_000393"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6333688.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGG"
     gene            complement(12884..13894)
                     /gene="rhuM"
                     /locus_tag="ACFM8U_000394"
     CDS             complement(12884..13894)
                     /gene="rhuM"
                     /locus_tag="ACFM8U_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HFC6333689.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTVAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(13891..15435)
                     /locus_tag="ACFM8U_000395"
     CDS             complement(13891..15435)
                     /locus_tag="ACFM8U_000395"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC6333690.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIVVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(15508..16512)
                     /gene="rfaD"
                     /locus_tag="ACFM8U_000396"
     CDS             complement(15508..16512)
                     /gene="rfaD"
                     /locus_tag="ACFM8U_000396"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC6333691.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(16553..17672)
                     /locus_tag="ACFM8U_000397"
                     /pseudo
     CDS             complement(16553..17672)
                     /locus_tag="ACFM8U_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(17674..18908)
                     /locus_tag="ACFM8U_000398"
                     /pseudo
     CDS             complement(17674..18908)
                     /locus_tag="ACFM8U_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            complement(18945..19916)
                     /gene="hldA"
                     /locus_tag="ACFM8U_000399"
     CDS             complement(18945..19916)
                     /gene="hldA"
                     /locus_tag="ACFM8U_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC6333692.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHEGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNEGEL
                     TEKAQNLRRHLDLTAILLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGVG
                     LGLAAGYTMPEAMYLANTAAGVVVAKLGTAVCSFAELTKALSGQSTM"
     gene            complement(19972..20712)
                     /gene="pyrF"
                     /locus_tag="ACFM8U_000400"
     CDS             complement(19972..20712)
                     /gene="pyrF"
                     /locus_tag="ACFM8U_000400"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC6333693.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRSLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNIAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPLAVLREVNRVANLEAN"
     gene            complement(21238..21885)
                     /gene="adk"
                     /locus_tag="ACFM8U_000401"
     CDS             complement(21238..21885)
                     /gene="adk"
                     /locus_tag="ACFM8U_000401"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC6333694.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            22100..22939
                     /gene="htpX"
                     /locus_tag="ACFM8U_000402"
     CDS             22100..22939
                     /gene="htpX"
                     /locus_tag="ACFM8U_000402"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC6333695.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FSGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
BASE COUNT         5365 a         5868 c         5629 g         6135 t
ORIGIN      
        1 acaggtattg gatgtggtat cgttcgcctt gggtcagttg cgtgtagctc atggcaatct
       61 ttcttgcagg aaaggtcgta tgctaccgca tactggcctt tttctgttag ggaaagttgc
      121 acttcaaatg cgaatccgcc ggagtctgaa atttgatgca ccttatatat tgcgtcccta
      181 agaaaggatg attaacaaaa attaacgccc tttactttct tttccgattg ggaacattcc
      241 cgtcgtacta acaggcaaat gaaggaacag aaatcacatt caaacggcgt tccgccgcct
      301 ctacaccgct tcccagtaat cgatatacag ctgcaattcg agattgttgc gccattcgtt
      361 ggcaacgggg cggtaaaccg tgcggatata ttcggggatg tcttcgctgc aacgccaaaa
      421 catggcttca aattcgcagc cgtctttttg cagccacgct tttttgtgtt taccctccgc
      481 gcccaaaggc tgctggcgga cgacgtgaaa ttcgtcggta aagcttggcg gcgcgaagcc
      541 ctgcccccaa acgtgtcggg caaggttttg agcctgttcc agcgtgatgt cgcaggcttt
      601 caggctgccg tcggtgatga aggtttgcga caaatcgtct tcgcacacca tctcgcgcac
      661 ggcttcttca aaggccgtct gaaacgcggg gatattgtgt tcgagtatgc tcaagcccgc
      721 cgccatcgcg tgtccgccga atttcaaaat caaatcggga tggcgtttgg acaccaagtc
      781 caaagcatcg cgcaggtgta gattgggaat ggaacgcccc gaaccgcgca cttcgccgtt
      841 gtcggcaggc gcaaacacga tggtcggacg ataaaaacgg tctttgaggc ggctggcaac
      901 gatgccgacc acgccttgat ggaagtcgtc gcgatacacc gccaaagccg tctgacctga
      961 aggcagggtt tcaggaaaag cattcagcgc gtcttgcagc atagattgct cgatttcgcg
     1021 acgctcgata ttgaggttgt ttaactgagc cgccagttcc tgcgcctcgg catcatctcg
     1081 cgccaacaga caggcaatac cgaccgacat atcgtccagc cgtccggcgg cgttgatgcg
     1141 cggccccaac gcaaagccca tatcaaacgg ctgcgccttg cgccaatcgc gccgcgccac
     1201 ttcaaacaag gcgcggatgc cggggcgcat tttgccggag cgcatccgtt tcaaaccttg
     1261 cgacacgagg atgcggttgt tgtggtcgag agggacgaca tcggcaaccg tgccgagtgc
     1321 gaccaaatcc aaaagttcgc ccaaattcgg ctctttgatg ccgtctgaaa aataattgcg
     1381 gcggcgcaat tcggcacgca acgccatcaa tacataaaaa atcacgccca cgcccgccaa
     1441 gcttttgctt ggaaaaccgc agcctttttg gttcggattg acgatgatgc agtcgggcac
     1501 ggtctcggca ggcaagtggt ggtcggtaac gatgacatcc aaacccagag cctgcgcgcg
     1561 cgccacgcct gcgatgctgg caatgccgtt atcgaccgta atcagcaaat ctacaccttg
     1621 ctctgccgcg atttcagcca gctcgggcgt taagccgtag ccgtgctcaa agcggttggg
     1681 cacaaggaaa tccactttcg cgcccatcgc cgccaaaccg tccaaaccga cggcacacgc
     1741 cgtcgcgccg tcggcatcat agtcggcgac aatcaggatt ttttcctgac gctcaaccgc
     1801 atccgccaaa cggccggcgg cggcttcgca attcgtcagc gattgataag gcaggaggga
     1861 agcgagtttg tcgtccaatt cggcaggact ttgcacaccg cgcgaagcac aaagccgggc
     1921 gattaaagga tcggcaccgg cggtgagcaa atgattaaaa acgtcggtat tgatggatcg
     1981 ggtttggatt ttgactgaca taacgtgggg ttttactgtg cgtgtgtgct taaaatgccg
     2041 tctgaaagct tttcagacgg cattgctgtt catttgcggg cggcatccac gaccgcaccc
     2101 agagccgccg caggcaaggc cagtaccgcg cccgccgcat ccagtatcaa caaggggggc
     2161 gtatgtaaga tattttcaaa cagcttggat ttgtcggtat gttttttcgt aaccgtgtaa
     2221 taaatatcgg caggcacact ttgctcaaaa tgataatcgg cgttcagttt ttgcggtgtg
     2281 gcgtagtatt tgcctttggc ggagacgcag cgcgtgtaaa tggtccgatt gtcgagttcg
     2341 accgctttaa actcaagctg tttcagcttg gcgatgtcag caggcctgtc ggtatcgtag
     2401 cgcaggcaaa ggccttcggt actgaaattc tggctgccag gcgattcgag tttgaccggc
     2461 agggcttggt ggcgagcata gctcggggta tcctcaacta tttggaaggg tttgtccagc
     2521 cctgccttca aaatgcccgt cagcttcgcc gaatcttcgg gattgacgac gaaccagtat
     2581 ttcccgccca tcatcaccag actgcccttt tccaattggg cattgtcttt ggcaaccaca
     2641 ccgaaggcgc ggatttgatc cttgtcaacg tgtttatagg cggtcgtttg gctgaacggg
     2701 tcgttcatgc cccacatcat tgccgtacag ccattcagca tcaaggttgc cgccaaaacg
     2761 gcggcggtcg ttttacggaa caacatttta tcctccaatc taaataacgg cggatgtcgt
     2821 ctgaaaatat gcctaaattt ccgcgtcccc gttttccatc acggttttca attcttgaaa
     2881 cagcgcgcgg aagtttttcg gcggtttgtt ttgctcctgc tcttttttgg tgttgcggac
     2941 gagcgtcctc agcttgcccg cgtccgcatg cggaaaatcc gacataaact gcgtcaacgc
     3001 gccgtcgtct gccaacagcc gtacgcgcgc ctgttccaca cgttgcaaaa aggcattgtg
     3061 caccgcatcg tcgccgcgca gcttggcaag aaacgcctcg atgggcgcgg gatccgtgtc
     3121 gcgcatcagc ctgccgataa attgcgcctg ccgcttgagc gcgccgttgg acgtaatttt
     3181 tttataggta acgacggctt cgtacaaatc ttcgtccaag ccgatttttt tcagcgtgtc
     3241 gtttgagagc ttggtcagtt ccatacccaa atcctgcaaa ccgttcatct gctttttcat
     3301 ttgggttttg ctgatccatt cgtcttcttg ttcaaacatc ttgattctcg aattttttcc
     3361 aatagcgcat tttaacagaa tgccgccgcg ttcagacggc attttgcacc gcacgccctg
     3421 caagcggcgc gcttttgccc ctgccgcccg aaacggttaa aatgccgcaa agcatcatca
     3481 agaaagattt ctatgctgtt caaccacacc gcttccgaac tcctcgacct ctgccgccgc
     3541 acgctcgact tggcaaaagc gacgggcgca accgccgccg aagccgattt cagcgaatca
     3601 ttgggacaaa gcgtcagcgt acggctgggc gaaatcgaac aaatcgagtt ccagcaggac
     3661 aagtcgctgg acattaccgt ttacgtcggc aaacgcaaag gccgcgccag cactgccgac
     3721 ttctccgaaa aagccctgca agacaccgtc aaagccgcca tcgacatcgc ccgctacact
     3781 gccgaagacg attgcgcagg tttggcagac gcttcgctta tggcgcaaca catcggcgac
     3841 cccgaccttt accacgaatg ggatttggat acggaagccg ccgtcggctt ggcaaaacaa
     3901 tgcgaacaag ccgccctgaa cgaggatgag cgcatcgaaa actccgaagg cgcggcggtg
     3961 caaaccggcc attaccaata cgtttacggc aacacccacg gttttgccac acaccggcaa
     4021 ggcacgcacc acagcatttc ctgcagcgtc gttgccgccg acgaaaacgg gatgcagcgc
     4081 gactactggt acgattccgc ctgccgccat ccggatatgg acagccccga aaccatcggt
     4141 caaaccgccg cccgccgcac tttgcgccga ctcggcagcc gcagcatccc gacaggcagc
     4201 taccccgtcc tcttcgatac caccgtttcg ggcggtctta tcggacacct cgtcggcgca
     4261 ctctccggcg gcgcgctcta ccgccaaagc agtttcctga tcgacagcat cggcaaaaaa
     4321 gtcctgcccg atttcctcaa cctgcgcgaa gaaccgcaca tcccccgctc tttccgcagc
     4381 agctattttg atgcggaagg cgttgccacc gcaccgcgct tcgtgattca aaacggcatt
     4441 gtcaaaggct atttcctcag cagttacagc gcgcgcaaac tcggtatgca gaccacgggc
     4501 aacgccggcg gcgcgcacaa cctgtatttg aaccatacgc acgaaacgca atccgacctg
     4561 ctgaaagaaa tggggacggg attattggtt accgaactga tgggacaggg cgcgaacacc
     4621 attaccggtg actactcgcg cggcgcggcg ggtttttggg tggaaaacgg cgtgattgcc
     4681 taccccgtcc acgagattac cgtagccgga cgcttgcagg atatgtaccg cgacattgtc
     4741 ggcgtggcgg atgacgcttt gcggcgttcg tccaacaaaa tcggctcgat tctgattgcg
     4801 gggatgacgg ttgccggaag ctgaacgccc tgccgatgcc gtctgaagac gaaggcagca
     4861 cgttttgccc cgtgcctacc cgtttgccgt tttatgccgc accgcctttt gccgacaaca
     4921 ttgcacattt tttcatcagc actgattttc ccttatccgt caaaatacta agccacttgt
     4981 ccacaacagg gtacacacaa gttcaggata aggcgtgcgg ggattggggt ttttccgccg
     5041 tcggcaaaac ggcagacaaa aacggcggga cattgcctgt cccgccgttt tcaaatatac
     5101 atttgaccgt acatcaggaa ttaagcagcc tgaatgttgg cggcctgttt gcctttaggg
     5161 ccggtggtta cgtcgaaaga gacgcgttgg ccttctttca gggttttgaa accttccata
     5221 ttgattgctg agaagtgagc gaacaaatct tcgccgcctt catcaggcgt gatgaaacca
     5281 aaacctttag cgtcgttaaa ccattttacg ataccggttg ccattagaaa cttcctatac
     5341 tcaaaaatta acaaaatcag caaaacaagg catacggcaa accgagccgt ctggcgttcc
     5401 ttccagcttg atatactcgg cggatgccca atcctgctta accgtctttt tacatttgtt
     5461 aagacaaaac gtcaagctat gttttcaaaa aagtgggaaa ataggcaaaa gtaccgaaaa
     5521 tacaacaaaa atgccgtctg aacgcggctt tcaaatggga cacactatga ccgcgcaaca
     5581 ccaatccgac acgctcttgc accgtctgaa caccctgccg ccgaaacgtt acggcgtttt
     5641 cctattgaac gacgattaca ccacgatgga atttgtcgtt gaaatcctga ccgaaatctt
     5701 tatgctcgga caagaacagg cggtagcggt aatgctcttg gttcatcacg aaggcaaagg
     5761 cctgtgcggc acttacacgc gcgatattgc ccaaaccaaa cagcaacaag tcatgcagcg
     5821 ggcaaaagcc gaagggcatc cgctgcaatg tattgtcgag gagatttaat atgcttgcac
     5881 ccgaattgga acagattttg cagcagcttt accgcgaggc gcgtaaggct cattatgaat
     5941 ttatcagcct cgagcatctg cttttggtac tcatcgaaga agatgccgcc gtgccgaacg
     6001 tcttaaaact ctgcggcgcg gatttgaaag tggtgtccga acagctcgcc gccagcgttg
     6061 ccgaaaacac ccccctgatt cccgaacacc ttttagacac ggtcgaaacc cggcccacgc
     6121 tcggcttcca acgcgtgatg caacgggcga tggtgcatac ccagtctgcc ggaaaagccg
     6181 cagtcgaacc gttggacgtt ttggtcgcgc tgatgagcga aaccgacagc cacaccgtct
     6241 atttcctcaa gctgcaatcg gttacgcgtt ttgaagtttt gcgctgtatt gcccacggct
     6301 ctcccgatga agatgaagat gaagacgatg gcaactattc ttcagacggc atggacgacg
     6361 ataatgaaaa ccgcaccaaa ccgggcaaaa accctttatc ggagtacacc gtcaacctca
     6421 acgccgaagt caaagccggc cgcatcgacc ccttaatcgg cagaaaacac gaaatggaac
     6481 ggctggtgca aatcctgtgt cgccgccgca aaaataatcc gcttttggtc ggcgaggccg
     6541 gcgtgggcaa aactgcgctg gcggaaggtt tggcacatca aatcgtcaac ggcgacattc
     6601 ccgacgcgct taaggaagcc gaagtgtacg cgctggatat gggttcgctt ttggcgggca
     6661 cgaaataccg cggcgacttt gaagcgcggg tcaaatccgt cttgaaacag ctcgaaaaaa
     6721 tcccgcacac cattttgttt atcgacgaaa tccacaccat catcggcgcg ggcagcacca
     6781 gcggcggtac gatggacgca tccaacctgc tcaaacccgc attggcgaaa ggttcattgc
     6841 gctgtatcgg cgcaaccact tacgacgaat accgcaccat tttcgacaaa gaccacgcct
     6901 taagccgccg tttccaaaaa atcgacgtgg tcgagcccac cgtcgccgaa accgtacaaa
     6961 tcctgcgcgg tttgaaaccg atgtttgaag atttccacca agtgcgctat acgcaaggcg
     7021 cactcgaagc tgccgccgaa ctctccgccc gctacatcaa cgagcgtttc ctgcccgaca
     7081 aagccatcga cgtaatggac gaagcaggcg cggcacaacg gattctgccc aaatccaaac
     7141 agaaaaaagt cattggcaaa gcgcaaatcg aaaccgtcat tgccaaagtc gcgcggattc
     7201 cagaaaaaac cgtgtcgcac gacgacaagc aagtgctgca attcctcggc cgcgatttga
     7261 aaaacatggt ttacggtcaa gaaaatgcca tcgatgcgct ggttgccgcc gtcaaaatgt
     7321 cgcgctccgg ccttgcccta cccgacaaac cgataggcag tttcctcttc tccggcccga
     7381 ccggcgtcgg caaaaccgaa gtcgccaaac agcttgccta ctcgatgggc gtaccgctgc
     7441 aacgctttga tatgtccgaa tacatggaac gccacgccgt atcgcgcctc atcggcgcgc
     7501 caccaggcta cgtcggcttt gaacaaggcg gccttttaac cgaagccgtc aacaaacagc
     7561 cgcattgcgt attgctcttg gacgaaatcg aaaaagccca ccccgatatt ttcaacgtcc
     7621 tcctgcaagt gatggatgcg ggcaggctga ccgacaataa cggcaagagt gccgacttcc
     7681 gcaacgtcat cctgattatg accaccaacg caggcgcgga aagcctcagc cgccccagcc
     7741 tcggctttac cgccaaccgc gaacgcggcg acgaaatgca ggccatcaac aaactcttca
     7801 cgcccgaatt ccgcaaccgc ttggacgcaa ttatcccgtt tgcgcccctg tccgaaccca
     7861 ttatcgccaa agtcgtggat aaattcctgc ttcagctcga acaccagctc ctcgacaaaa
     7921 aagtcgaagc cgaattcacg ccggcattgc gcaaatatct ggcggaaaaa ggtttcgacc
     7981 cgcaaatggg cgcgcgcccg atgcaccgcc tgattcagga aaaaatccgc aaaccgctcg
     8041 ccgacgaact gctgttcggc aaactcgccg acggcggctt tgtgcggata gactgggatg
     8101 ctgcaaaaga agaagccgtg ttgaaattca agaaaaacaa agtcaaacct gaagcagaac
     8161 ctgtttaacc agttgaaaaa taaaaagccg tctgaaaggt tcagacggca ttttgtcaaa
     8221 tatcggtccc tattccggca aacttctcca caaacgccgc aatcttttga aaaacacttt
     8281 gtttcttcgt cagatattgc gggttgagcg gactcatttt cggcagggtt tcggtcagtt
     8341 ccgtaccgtt ttcgctggca tagccgcgtt tgagcgagcc ggtcaaatag cgtttggcgg
     8401 cggtttcgtt cagaccttcg gcggcaatca atcctgccgc ttcgtgccgc atcacctctt
     8461 gcgcgtaggt gtagaaggtt tccagtatgg cgggaacgtc ggggactttg tccaaatccg
     8521 tatcgttgat gaaatccaca atcagaccct ctttggcgcg gtgtccgata cttgcgcgga
     8581 tgatgcggcg gatttcttcc accagctccg ctttgccttt gatttttttg tggtgctcga
     8641 aaaccagttg caggatgtaa tccaaattga tttcctgcga tttgagcaaa tccacctcaa
     8701 aaaccacatc gtcccagtcg atttttgact gttctttctg ttcgcctgct ttttggcggc
     8761 gcaaccaatc gcggatgtcg ttgtaggcgg aacggtagtc ctgcaccgcc ctttcagacg
     8821 gcatcggcac ttgccgcatt tcctgcacgt cttcatcgct caggtagtat ttttcttgaa
     8881 acgccttcat cgcatcttcg tccgccgcgt ccacactctg caactcgcgc agcgcggcaa
     8941 attcatcgta gttctgcaat acgttttccg cccgcaggta ttcgccgaag agtttggcaa
     9001 aatctttttt gtctttttcc gtttcgattt tgtcgggttc ggggaaacgc tcgcgcaatt
     9061 cttttgccac atccaaatag ccgcgccgtg cttcgcccgt ccgatcatcg atatagccgt
     9121 tcatgtattc ttcgtaactt ttttccagca ctacattttt ggtgtttttg tcgccaaaca
     9181 aggtaatcgc atcaatggtt gcctgctcca aatcgcggaa gcagacaata ttgccgaagg
     9241 ttttggtggc atcgtaaatg cggttggtgc gcgaaaacgc ctgcatcagg ccgtgatagc
     9301 gcaggttttt atcgacgaac aacgtgttca gcgtcggcgc gtcaaaaccc gtcaaaaaca
     9361 tgccgaccac aatcagcaaa tctatttcct gatttttcac ccgttttgcc aaatctcggt
     9421 agtagttttg aaaggctttg ctgtccgtgc cgaaattggt tttgaaacag gcgttgtaat
     9481 cgttgatggc agcctgcaaa aattcttttg cgctgctgtc catcgcttcc ggttcaaaag
     9541 tctcatcgac aatttcaccg acggcgtttt gctcttcgtt ggccgcaaag gaaaaaatgg
     9601 tggccacttt cagcgggtgc aagctgcctg cctgttgtgt tttgaacgct tcgtaatagc
     9661 acttcgccgc atccacgctg ctgacggcaa acatcgcgtt aaagcctttg ccacccgcat
     9721 tcagccggtg cgttttctgc ctgaactggt tcaggatata ttgcgtgatt tcgcggatgc
     9781 gttcggggtg cagcagggct tggcggtttt cggcggcact cagtttcttc tcgtcctgtt
     9841 ccgcttccac ggctttgaac tgcgggcgca catcgttgta atccactttg aatttcaata
     9901 ctttttcatc gcggatggca tcggtaatca cataagaatg caactcccgc ccgaacacgc
     9961 ccgccgtggt ttccgcgccc aaagcgtttt cgggaaaaat cggcgtgccg gtaaagccga
    10021 actggcagaa ttttttaaat ttctttttca ggtttttttg cgcttcgccg aattgcgagc
    10081 ggtggcattc gtcgaaaata aagacaaccc gctgatggta aatcggcaga ttatcttcac
    10141 tcttcatcag gttattcagc ttttggatgg tggtaacgat gattttgttg tcgtcttttt
    10201 ccaaattgcg tttcaagccc gccgtgcttt ccgaaccgtt cacgctgtcg gtcgaaaaac
    10261 gttggtattc cttcattgtc tgatagtcca aatccttcct gtccaccacg aagaaaacct
    10321 tgtcgataaa cgccgattcc gtcgccagat gcgccgcctt aaagctggtc agcgttttgc
    10381 cgctgcccgt ggtgtgccag acatagccgc cactctccgg cttgctccaa ttcttcgcct
    10441 gcgccgagct gttgattttc cacaaaatgc gttcggcggc ggcaatctga tacggccgca
    10501 taatcagcag cgtattattc gcatcgaaca cgctgtaatg cagcaaaacg ctcagcaata
    10561 cgcttttctg caggaacgtg tcggtaaagt ctttcaaatc cttaatcgga tgattgtccg
    10621 accgcgccca attcatcgtg aaatcgaagc tgtttttgtc gcgcttggtg gtgttggcga
    10681 aatagcgcgt gtccgtgccg ttggaaatga cgaagatttg caggaatttg aacaacgaat
    10741 tgtcgctgtt gaagctctct ttgctgtaac ggtgcacctg attgaatgct tcacgcaccg
    10801 ccacaccgcg ctttttcaat tcaatctgca ccagcggcag gccgtttacc aacacggtaa
    10861 tgtcataacg gtttgcatgc gtgcccgtct gctcaaacta gttgataagc tgcacatggt
    10921 tgcgggcaag gtttttcttg tccagcagat aaatgttttt cagacggccg tcatcaaaag
    10981 cgaaatcgta aatatggtcg tcgtggattt tgcgggtttt gtcggtaatg ttttcagacg
    11041 gcctgtccag atattccgtt aaaaaccgcg cccattcgcc gtctgaaaac gccacatcgt
    11101 tcagccgctg caactgcgcg cgcaggtttt ccagcagcct gctttggctg ttcaagtctt
    11161 tgcggtattc gtaaccctga ttctgcaaat cggcgattaa ctccgcttcc aactggtttt
    11221 ccgattggta gctgcccgac tgttcgattt tttcatattg gtcgagcacg atgaaattcg
    11281 gcgtttcagc gatgggttta gtttcgaggt tcatatgggt cgtcctttgc ggtgtcgggt
    11341 tttatagtga attaaattta aaccggtact gcgttggctc gccttgccgt actatttgta
    11401 ctgtctgcgg ctcgccgcct tgtcctgatt taaatttaat tcactataaa cggtttgatg
    11461 ttcggattgg gctgcctgaa aatgtgcagg ctgctttttc aggtagcctt agggaaggca
    11521 agcagctgtt cgcggtaata ttcgtattgt ttgcggcgca gggcgatttc gtgcggcagg
    11581 ccttcgctga tagagtgggt cagggtatcg aatttgtcta ggatggcggc aattttttcc
    11641 tgttcgggga gtggtgggat ggggattttt aatttcttta attctttcat gctaataata
    11701 ggtaaacttg aagtattaat aagttttttg ctctgctccg caataatgaa aaaataatag
    11761 aaagcgaaat ttatatctaa agcatcctta aattcatctt taattgtaaa attggtcagt
    11821 tgttggttac taatataatt aacttttatt aacgcatgct ctcctatggt tgccgttgtc
    11881 gacatcatta ttgatttagc aggaaacagc ttgccgccct taactgcgct ttttgatata
    11941 tgcttcaatg aattatctaa aatacggcca ttttccctaa tatcttccat tctaaaccaa
    12001 gggattgagc cattttccca atattcttta tttgatttgg aaggggtata gccattcttc
    12061 aagtcaaaca cctcccccaa cgtcttccaa accacatctt tcagacggcc tttatagcca
    12121 tcagctatcc ccccccgatt tgattgtcaa aatctaaaag aaagtcgcgg taataccggt
    12181 attggcgttt gcgcagggct aattcagctt ccagctcggt gaatttgtca agtattttta
    12241 caattttttg ttgggtttcc aggggtggga tagggattaa taattttttc aagttactgg
    12301 catttagagc aggaatacct gtaaatgtac ataaggcatg aatctcttgt tcaaattcaa
    12361 gtaatatatg atagagaaaa cgaacatcca attctgttct attcttaata gtaactgcat
    12421 aatttaagtt gccacgaaaa tatcttcctt cttgccacgt tattgagccg actcccgctc
    12481 ctcttgttgt gatgccgata gggtcatttt cagtgttcca ttcatcaata tagccaagcg
    12541 gctctttgcc actgttaata actggataac taccaatatt atttgagatt ttctgcttac
    12601 ttacagcttg tcctgttttt atgatagcta tcccattttc tccacccaac ggcttccact
    12661 ccaccggtgc cgtctgaatc atctcaatca attttttcgc tttgctttgc atatccatgg
    12721 ttgttttccg tttctttccc gctgtggcgg gatgtggggc ggttgtgggg tgcttcggct
    12781 cactctgtcc gtttgccgtt atttctgcgg gctgaagtcc gtcctgcttt caggctgcct
    12841 gcaatggtgt gcaggctgct ttttcaggta gcctgaatgg tagttatttt gtgcggtctt
    12901 ttttctgttt gatctgttgc acggctgttt tttccaacgc tttaacgctt tccagatagg
    12961 cttgctccac cggcgacagg atgcgcgctt cataggcgcg gtattcgcgt tcggctttct
    13021 gctctgcctg tttgcggctg atgctgcctg caccctgtaa tgtgccttgt ccgtacagcc
    13081 cggaaaattt gtccaattcg gctatccagt cgcgcatata catggggctt tgctcctgcg
    13141 ctttgatttc cgccaggtcg aagaaggcgg aaaccagacg gttcaggcgg aacagttcgt
    13201 cttcggtcag atagtttttg gcgattttgg cttcattcag cgtggggatt gcgccttgaa
    13261 aggtggtcag ccccataaag tctttgctgc tgtcggcacg gctgtatatc agctcagcta
    13321 cggtttgccg gctggcggca tagtgcagtt tgttttgaac ggcggcaaaa aaggtttggc
    13381 tttcgctgct tttggggttg tagtcttggc tggttgcata taaatcaagc acttgccggt
    13441 atagggcttt ttcgctgctg cggatgtcgc gaatgcggtt gagcagttct ttccaatagt
    13501 cgccgccgcc tgtgcctttc aggcgttcgt cgtctatggc aaagcctttg gtcagatatt
    13561 cgtccagccg ttcggttgcc cattggcgga attggatgcc ccgcgcggaa cggacgcggt
    13621 agccgacggc aataatcatg ggcagggaat aatgggcgat tttgcggttt accttgcgcc
    13681 cgttttcatt ttgaactgtc aactggaagt tgacagttgc cttctcttcc aattcttgct
    13741 ctgcaagaat ggttttaatg tgtttgctga tattttgttt ggtggtttgg tacagttctg
    13801 ccatgtccgc ctgcgtcagc caaagctgtc cgccaaattc ctgcaaggca aattgggcag
    13861 tgccgtcgtt ggcggtgtat aggatgatgc tcatgcttca atctctgcaa tcacttcgtc
    13921 aatttcacgc cgcagccgtt cgattttggc aacggtttcg ctgatttcgg cgttaagctg
    13981 tttgatgtcg ataatttcgc gggtgtcttc gggttcaaca tagctgctga cggcgaggtt
    14041 gtagccgttg tctttgacgg tttgctgggc ggcgttttgg gcgatatgcg gcacatcggc
    14101 tttatcggcg aagagtttga cgatttcggc aatgtgttct tcggttaaga cgttgttgtt
    14161 ggtttctttt ttaaagaagc cgcctgcgtc gatgaattgg atgtcggtat tgtctttgtg
    14221 tttggacaaa accagtatat tgacgacgat gccggtgccg taaaagagat tgggcgcaag
    14281 ggcgatgacg gtttccacgt agttgccctc caccagatat tggcggattt tctgctctgc
    14341 gccgccgcga tagaaaatgc cggggaatga gacgatggcg gcgcggcctc tgccagaaag
    14401 gtagttcagt gcgtgcagaa tgaaggcaaa atcggctttg gatttcgggg cgagtacgcc
    14461 tgcaggggca aagcggtcgt cgttgattaa ggtggggtcg ccgctgccta tccagttgat
    14521 ggaatagggc ggattggaaa cgacggcatc aaagggtttg ctgtctttga gtttgggatt
    14581 ggtcagtgtg tcgcccaatt cgatgtggaa tttgttgtaa ttgacgttgt gcagaaacat
    14641 attcatgcgg gcgaggttgt aggtggtgtg gttgatttcc tgcccgaaga agccttcttc
    14701 gatgatgtgc tcgtcaaact gttttttcgc ctgcaagagc aggctgcccg agccgcaagc
    14761 tgggtcgtag attttgttta ctttctcctg cccgtgcacc gccagccgcg caatcagctt
    14821 ggatacgctt tgcggggtga aaaattcgcc gccggatttg cctgcgttgg cagcgtagtt
    14881 ggaaatcagg tattcgtagg catcgccgaa aaggtcgatg tggtggtctt caaaactgcc
    14941 gaaatcgagt tccgccacgc cttttaggac ggcggcaagg cgtttgttct tgtcggcaac
    15001 ggtgctgccg agccggctgc tggtggtgtc gaagtcgtca aacaggcctt taatgtcttg
    15061 ttcggacgga tagccggagg cggagctttc aatcgcggta aaaatttctt tcagcttggt
    15121 gttgagctct tcgttttgat gggcttcggc ggcaatattg caaaaaagct ggccggggta
    15181 gatgaaatag cctttgactt tgacggcatc gtctttgatt tcgggcgtga tgatgctgtc
    15241 tggcatagcg gcgtaatcaa tactgctgtc gcctgcctgc atatagtcgg taaagttttc
    15301 gctgataaag cggtagaaaa gtgtgccgag aacgtattgt ttaaagtccc agccgtccac
    15361 cgcgccgcgt acttcgtcgg caattttcca aatttggcgg tgcagttggg cgcgttgttg
    15421 catttcggtc atcatcgaaa tccatataaa agttaaacaa atcgaaatcg cctgatattt
    15481 tcagacgatt ttttacgggc attcaaatta agccaaattt tccagcatcc atttgacgta
    15541 gcgctcgacg cctgatttga catcgaaaaa ttcttccgta taacctgcct cacgcaattt
    15601 ggtgatgtcg gcttgagtga agctttggta tttgcctttg agcgcatcgg gaaatgggat
    15661 gtagcggata agttcttctt ctaccagctc tttcaagctc atttcgggtt tgccttcggc
    15721 ggcgcggcag gcgttgacgg tggcggcggc gagttcgtta aactgttggc tgcggccggt
    15781 gccgaggttg tagatgccgg aaagttcggg atggtcgaag aagtagaggt tgactttggc
    15841 aacgtcttcg acgctgacaa agtcgcgggt ttgttcgccg ttgccgtagc cgtcgttgct
    15901 gccgaacagg ttgacgtaac cgtgttcgcg gtattggtgg aaatggtgga aggcgacgga
    15961 tgccatacgg cctttgtgtt gttcgtgttg tccgtacacg ttgaagtagc ggaagccgac
    16021 gacttgggcg gtcaagcctt ctttcatgcg gcggcgcaat acttggtcaa acaggaattt
    16081 ggaatagccg tacacattga gcggtttttc gagttcgcgc tcttcacgga agatttcgcc
    16141 tttaccatac acggcggcgc tggaggcgta gaggaaggga atgcgttcgt cctgacacca
    16201 gtccaacaaa tccagcgtgt actggtagtt gttttccatc atatacaaac cgtcgtgatt
    16261 catggtgtcg gaacacgcgc cttgatggaa aacggcttcg atgttttgat aaggtaaaat
    16321 gtgttccctc acttggcgga tgaattcatg tttgtcgaga taatgggcga tttcgcactc
    16381 ggcaaggttt ttgaattttt cgcctttgct caaattatcg acggcaacaa tgtcagtaat
    16441 accgcgttga ttaagtgctt tgacgatgtt gctgccgata aagccggccg cgcctgttac
    16501 gatgatggtc atattcggtt tcctttgttt tgagtaaaat taataaactt agttacaaga
    16561 aatcatcgat attgtcctga aacacaaatt tgcgatgata ttccaaatat ttttctcttc
    16621 cttcgggata tattctcatc ttaatttttt gcatttggat tgatattaag tttttcccat
    16681 gtgtaacggc ttagtcgagt accgcaaatc aaacgaccat catccaagaa agtaatataa
    16741 ccttgatcga acaaccaatc ataagtcggc gttaaagcta acccatttaa ataatctaat
    16801 gcctctttct catttttctc agtaatacaa accatataag gtttaatatg actagccctt
    16861 aacaaaatct catctgtaat taatgtaaat ggacattgcg gcatatgatt tataacatcc
    16921 ttgcgatatt tctgctgccc ttttctactt ttctgtttaa tgatttcttg ctctacgaat
    16981 tgattaaatt ctgaagccgg tatttctaat ttttctcttg ataagagttg cgggtgcacg
    17041 atagagcgaa attgataatc caaaaatatt cggaaataaa ataatggttc tgaatcttct
    17101 atatctttga caggcaatag tttaagaatt gataaataaa taattttggg caatacaatc
    17161 ttacgccaaa ttgaccatat atcatcctcc gaacggatat aacccctacg gtaaccttta
    17221 gaattttcca aatggtctga tacatcatgg attgaaaaga aaatattttc tgtaggcaga
    17281 ttattaatac ttaaaacgta atcatcataa aaccttgaaa tatcatgatg atatttctgt
    17341 tcttgtttgt gatattcaac ttttgcatca accaaataac gctgtaaatt tttcttatta
    17401 aaaaaacaat ttttctgaat gaaaggatat tctaccctcc ttccattttt tgtctcagta
    17461 atcccccaat tttcatactc aaaaaatgta cttagttttc gaccactatc atcaatataa
    17521 ctacctacat atttgcgtgc ttccccatta ccaccaaaaa cagataactt gttattcctg
    17581 tgaataaaac tatcttccgc acgtaaattt tgaatactat cgacaataaa atactctatg
    17641 ttttcaatag taattctttg aaaatccggc attttactct ccataaagcg taatatccat
    17701 ttgttttaat atagcgatta atacatcaac aacaatactg tttcccgctt ggcgatacat
    17761 ttgagtatca ctaactaaaa ttttaaaatc atctctaaaa cccattagcc gcaaacattc
    17821 cctaggtgtt aatttacgaa tacgtccacg atcatgagta acataattat caaccccggc
    17881 acgatgcatt ttatgcatag tttgcaataa tgggcgagct acaggcaaat cagtttccgt
    17941 actggtttta aaattttttg ttcctcctgc caaaacataa tttttgattt tttcagaaag
    18001 ataatatttt tcctcaacgt tatttacatc aaaaataaag tcatcaaatt cagattcgcg
    18061 ggctgcctga aaaataaaat caccatgcca attaaattgt tgatttgctt tttggcataa
    18121 ggcaatttct ccattaatct gtgtataacg tttttgtcta tttttagaac tggtaacaaa
    18181 tttcgcaccc ttttcacgta aaaaatattt gctatcagta tagtcctcca aaaaatcttg
    18241 catagtatgt tctaattcaa tcttttcagg aaactgaaaa ccatttatag gaggggtatg
    18301 aaagccaaca acaaaaatac gctcacgatg ttgaggaatc ccataatcct tactattcat
    18361 tatttggaaa tataagtcat aaccaagtga ataaaaaaca ctttttacaa ctttccaagt
    18421 ttttccatta tcatgattga gcaagccctt tacattttca taaataaata tctttggttg
    18481 gacttcatcc acaacgcgag caaattcata gaataatgtt cctcgtgtat cttctaatcc
    18541 tgcacgtttg ccaaccatgg aaaatgcttg gcaaggactg cctccaacta aaatatcaac
    18601 ttgatttcta aactttctcg catcaaattg agtaatgtcg ttatgccaaa aatcttcatt
    18661 taatttatag tttccaagat aactttttta acgtatggat caatatctcc tgcaaaaaca
    18721 atggtatggt ttaaatttaa tcggtgaaat gcctgttcaa ccgcaccaat cccgctgaat
    18781 acggttgcta atctaatatg tgaatcaggt ttaagaaaag ttttagattg ccaaccttgt
    18841 tgactgggaa agagcaaagt tttttgtaat cgagtatcgt gtgtctgtgc cattgtcgaa
    18901 atagtcatac ttatatcgtt ctgtttatct tatcaatatg aaaactacat tgttgattgc
    18961 cctgacaatg ccttggtcaa ttcggcaaac gagcaaaccg ccgtaccgag tttcgccacg
    19021 acaaccccgg ccgcagtatt ggcaaggtac atggcttcgg gcatggtgta gcctgccgcc
    19081 agccccaaac ccactccggc aataacggtg tcgcccgcac cggatacgtc gtaaacttct
    19141 tgggcgcggg tgggctggta aatcggttcg ccttcactga acaaggtcat gccttcttcg
    19201 cttcgggtca gcaaaatcgc ggtcaaatcg aggtggcggc gcaggttttg cgctttttcg
    19261 gtcagctcgc cttcgttttt ccaactgccg accacttctt tcaattcggc gcggttgggc
    19321 gtaatcagag ttgcgccgac atatttttcg taatcgtcgc ctttggggtc gattaatacg
    19381 gttttgcctt cgtgtttcgc ccaatcgatc atatcggaga tgtgcgacag gccgcctttg
    19441 ccgtagtctg aaaaaatgat tgcgtcgtat tcgggcaaga tttcgcggta tttccgcttg
    19501 atttgttcca acacttcgcg gttgggatgt tcttcaaaat caaggcggat aagctgctgg
    19561 ttgcgggcga cgacgcgcag tttgacggtg gtggcgattt gtttgtcgcg catcagatag
    19621 gaggcaacgc cgtcctgcac catcagcgcg tcgagcgcgt cggcggcttc gtcgtcgccg
    19681 gttaccgaca acagccctgc cctgccgccc aacgaagcga tgttgcgcgc aacgttcgcc
    19741 gcaccgcccg cgcgttggtc gatacgtccg atttttgcca ccggcacggg ggcttcgggt
    19801 gaaatacggg acacgtcgcc aaaccaatag cggtcgagca tcacgtcgcc aacaaccaga
    19861 actttggctt gtgcgaaacg ggatttgagg gtttcttgtt gaaacttggc gggcatattg
    19921 cctccgttgt gttttgttca gacgacctta accggtaaga ccgcctgaaa atcagtttgc
    19981 ttcaaggttt gccacgcggt tcacttcgcg caatacggct agcggatcgg cagcttgggt
    20041 aacgggacgg cccatcacga gataagtcga acctgccgcc aaggcttcgg cgggcgtcat
    20101 gatgcggcgt tggtcgtcgt tgttgccggc aacgtccaag cggataccgg gcgtgaccaa
    20161 gacaaaatct tgtcccaatt cgcggcgcag cggtgcggct tcttgggcgg agcagaccac
    20221 gccgtccaag cccgaacttt gcgccagttt cgccaaacgg atgacttgtt cttcgggagc
    20281 gatgttcaaa ccgatttccg ccaaatcgct ttgttccatg ctggtcaaca cggttacgcc
    20341 gattaagagc ggctttgtgc cgtatccggc aacggcttct gctgcggctt ccatcatacg
    20401 gcggccgccc gacgcgtgca tatcgaccat ccaaacgccc atgtcggcag cgactttgca
    20461 ggcttgcgcg acggtgtggg gaatatcgtg gtatttcaaa tcgaggaaaa gtttgaaacc
    20521 ttgattgatt aagctttctg ccaaactgcg tcccgtcgcg gtaaacagct ctttgccgat
    20581 tttgatttga cacaatgtcg ggtcaaggtt gcggacgaat ccgagcgtgt ctttttcgtt
    20641 ggcaaaatca agggcgacga tgacgggggt gcgttgttgc ggagtttgga agtcggagat
    20701 taaaggattc atggtgtttt cgtttcgggt tttcagacga cctttatcgt ctttttcgca
    20761 gtatagtgga ttaactttaa accggtacgg cgttgtctcg ccttagttca aagagaacgc
    20821 ttctctaagg tgctgaagca caaagtgaat cggttccgta ctatctgtac tgcctgcggc
    20881 ttcgtcgcct tgtcctgatt tttgttaatc cactataaca acgtaaattc aaaccgtagg
    20941 tcgggcattg atgtccgacc tacgcggact caagcctccg acaaactgtt tcggataaat
    21001 ttgaaccggt acatcagccg tcattcccgc gcaggcggga atccattgat aaaactcagt
    21061 tcatcagatt taaacagcga ttgcccaaat tcagaaatgg attcccgcct gcgcgggaat
    21121 gacgccaaat gattattttt cggttgcaac acgctgttta tcaaaaacag gtcgtctgaa
    21181 aacctgattt tctgttttca gagtgcgaag cctgcccgtg ggtacgacct tttcgattta
    21241 tttgcccaat gcgcccaata cttcggcttt cacggcttct accggctgag tgccgtcaac
    21301 tttgatgtat ttaggcgcgt gttcgccttc cagtttgctg taaaaatcga ccaaaacttc
    21361 ggtttgctcg tggtaaacgg caaggcgttt tttcacggtt tcttctttgt cgtcgtcgcg
    21421 ctgaatcaaa tcttcgccgg ttacgtcgtc tttgccttca actttgggcg ggttgtaggt
    21481 aacgtggtaa gtacggccgg aagccaaatg cacgcggcgg ccgctcatgc ggtcgacaat
    21541 cacgctgtca ggcacatcga tttcaacgac tgcatccaaa tccacgcctg cttcaaccat
    21601 cgcttcggct tgtgccaatg tgcgcgggaa accgtcaaac aggaaaccgt ttttgcagtc
    21661 gtcttgcgcg atgcgttctt tgaccatgcc gataatgatg tcgtcgcgca ccaagccgcc
    21721 ttcgtcaatg atttttttcg cttccaaacc caagggcgtg cctgccttaa tcgcggcacg
    21781 gagcatgtcg ccggtagaga tttgcggaat gccgaacgct gcggtgatga attgcgcctg
    21841 agtgcctttg cccgcgcccg gtgcgcctaa aagtaatgct ttcatgaagt gatcctttta
    21901 aatgtgttta ttggaatcaa acagatggtt tacgattcta cacggcttgg cacaatagtt
    21961 caaaaaccaa ctgcacaaac tgccatttgc aagcaaatgt tttgcatacc tttgccgagc
    22021 ctgatattca caaggcggca actttttggg acaatcccca aaatttcaaa acatcacacc
    22081 ctaccaaaaa ggaacatccg tgaaacgcat ttttctgttt ttggctacca atatcgctgt
    22141 tttggtcgta atcaacattg ttttggcggt tctgggcatc aacagccggg gcggcgcggg
    22201 cagcctgttg gcgtattccg ccgtcgtcgg cttcagcggc tcgattattt cgctgctgat
    22261 gtccaaattc atcgccaaac aatcggtcgg tgcggaagtc atcgacacgc cgcgcaccga
    22321 agaagaagcc tggcttttga acactgtcga agcccaagcg cggcaatgga acctgaaaac
    22381 acccgaggtc gccatctacc actcccccga acccaatgcc tttgccacgg gcgcatcgag
    22441 aaacagctcc ctgatcgccg tcagcaccgg tttgctcgac catatgacgc gcgacgaagt
    22501 ggaagccgta ttggcgcacg aaatggcaca cgtcggcaac ggcgatatgg ttacgctgac
    22561 gctgattcaa ggcgtggtca atacctttgt cgtgttcctg tcgcgcatta ttgccaacct
    22621 gattgcccga aacaacgacg gcagccagtc ccagggaact tatttcctgg tcagcatggt
    22681 attccaaatc ctgttcggct tccttgccag cttaattgtc atgtggttca gccgacaacg
    22741 cgaataccgc gccgatgcgg gcgcggcaaa actggtcggc gcgccgaaaa tgatttccgc
    22801 cctgcaaagg ctcaaaggca acccggtcga tttgcccgaa gaaatgaacg caatgggcat
    22861 cgccggagat acgcgcgact ccctgctcag cacccaccct tcgctggaca accgaatcgc
    22921 ccgcctcaaa tcgctttaaa ccgatttgaa acggcaaaaa ccgtacgcgc aagcagtacg
    22981 gttttttgta tatgctg
//
LOCUS       DAXHSJ010000014        22424 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000014 DAXHSJ010000000
VERSION     DAXHSJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22424
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.014"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            3..602
                     /locus_tag="ACFM8U_000403"
     CDS             3..602
                     /locus_tag="ACFM8U_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6333696.1"
                     /translation="MGISEVSNFEGRMRHLENNGYANVAGLERILAVKTDNYKEKENL
                     LHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKNETAESEFEKSVHERRQEGN
                     AGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSKIRLFKEAYFGKSGTRLTDE
                     IADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            639..1400
                     /locus_tag="ACFM8U_000404"
     CDS             639..1400
                     /locus_tag="ACFM8U_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC6333697.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            1455..1796
                     /gene="fdx"
                     /locus_tag="ACFM8U_000405"
     CDS             1455..1796
                     /gene="fdx"
                     /locus_tag="ACFM8U_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC6333698.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(2083..2670)
                     /locus_tag="ACFM8U_000406"
     CDS             complement(2083..2670)
                     /locus_tag="ACFM8U_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFC6333699.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWLIYTQIAERLAGSGGGFT
                     VTVESVSAACLELEGRYLELVRRAAVSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            2883..3080
                     /gene="iscX"
                     /locus_tag="ACFM8U_000407"
     CDS             2883..3080
                     /gene="iscX"
                     /locus_tag="ACFM8U_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC6333700.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(3148..3549)
                     /locus_tag="ACFM8U_000408"
     CDS             complement(3148..3549)
                     /locus_tag="ACFM8U_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6333701.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            3760..4719
                     /locus_tag="ACFM8U_000409"
     CDS             3760..4719
                     /locus_tag="ACFM8U_000409"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC6333702.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            4787..6097
                     /gene="tilS"
                     /locus_tag="ACFM8U_000410"
     CDS             4787..6097
                     /gene="tilS"
                     /locus_tag="ACFM8U_000410"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC6333703.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            6148..6933
                     /locus_tag="ACFM8U_000411"
     CDS             6148..6933
                     /locus_tag="ACFM8U_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC6333704.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGRPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLARYEDRVFATALREEKQAVLYNEDLREPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            6943..7140
                     /locus_tag="ACFM8U_000412"
     CDS             6943..7140
                     /locus_tag="ACFM8U_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333705.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            7402..7755
                     /locus_tag="ACFM8U_000413"
     CDS             7402..7755
                     /locus_tag="ACFM8U_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333706.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRFVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            7767..8072
                     /locus_tag="ACFM8U_000414"
     CDS             7767..8072
                     /locus_tag="ACFM8U_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333707.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            8134..9510
                     /gene="mpl"
                     /locus_tag="ACFM8U_000415"
     CDS             8134..9510
                     /gene="mpl"
                     /locus_tag="ACFM8U_000415"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC6333708.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQVGEVNADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKAALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            9717..10769
                     /gene="bioB"
                     /locus_tag="ACFM8U_000416"
     CDS             9717..10769
                     /gene="bioB"
                     /locus_tag="ACFM8U_000416"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC6333709.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLDDAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(10915..11481)
                     /locus_tag="ACFM8U_000417"
     CDS             complement(10915..11481)
                     /locus_tag="ACFM8U_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6333710.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQRPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKINPEKPLSGGLFI"
     gene            11829..12065
                     /locus_tag="ACFM8U_000418"
     CDS             11829..12065
                     /locus_tag="ACFM8U_000418"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6333711.1"
                     /translation="MCNPPKICYAFRTPSAYGQYLADAGFDYLSFANNHSNDFGAQGI
                     TATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(12128..13987)
                     /gene="ilvD"
                     /locus_tag="ACFM8U_000419"
     CDS             complement(12128..13987)
                     /gene="ilvD"
                     /locus_tag="ACFM8U_000419"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC6333712.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAGEIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDITNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(14160..15929)
                     /gene="cysI"
                     /locus_tag="ACFM8U_000420"
     CDS             complement(14160..15929)
                     /gene="cysI"
                     /locus_tag="ACFM8U_000420"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFC6333713.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVPEGGKARIEQLARSYGLIHDGVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFSIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(15956..17770)
                     /locus_tag="ACFM8U_000421"
     CDS             complement(15956..17770)
                     /locus_tag="ACFM8U_000421"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HFC6333714.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSVLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLLERVDADLDFTASADGWTGRIVARLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDQATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLFFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(17767..19053)
                     /locus_tag="ACFM8U_000422"
     CDS             complement(17767..19053)
                     /locus_tag="ACFM8U_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HFC6333715.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKEAIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDAHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVSAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(19092..20015)
                     /gene="cysD"
                     /locus_tag="ACFM8U_000423"
     CDS             complement(19092..20015)
                     /gene="cysD"
                     /locus_tag="ACFM8U_000423"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFC6333716.1"
                     /translation="MTKTEPNNVQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVTDISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(20053..20787)
                     /locus_tag="ACFM8U_000424"
     CDS             complement(20053..20787)
                     /locus_tag="ACFM8U_000424"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HFC6333717.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRFQP
                     IREDALHYVESKGRFAFYDSVEARRECCRIRKTEPLDRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIDKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEDKNSKECGLHK"
     gene            complement(20803..22254)
                     /gene="cysG"
                     /locus_tag="ACFM8U_000425"
     CDS             complement(20803..22254)
                     /gene="cysG"
                     /locus_tag="ACFM8U_000425"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFC6333718.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNTELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLREKLEALLPPSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGGRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKL
                     AWFGENAKKESNPAEHAYFALDGLGTGQEQQAA"
BASE COUNT         4861 a         5807 c         6445 g         5311 t
ORIGIN      
        1 aaatcggcat ttcggaggta agcaattttg aaggcagaat gcgccatttg gaaaacaacg
       61 gctatgcgaa cgttgccgga ttggaacgca tcctcgccgt caaaaccgac aattacaaag
      121 aaaaagaaaa cctgctccat gaaattttca gcaaaagcag gataggcgat accgaattgt
      181 tcgccgtgga cgaaaacctt gtaaaacgtt tgtttttatc gcttcgcggc gaaatcgtgt
      241 ttccgaaaaa cgaaacggcg gaatcggaat ttgaaaaaag cgtccacgaa cgcaggcagg
      301 aagggaatgc cgggtccggc cgcaaacaac tgcttgattt ggtacggcgc ggacaccggg
      361 aataccctta cgcgctgccc cggcttttgg cgggcgcggc atcctacaag ccgaaaaaat
      421 cgaaaatccg cctttttaaa gaagcatatt tcggcaaaag cggcacgagg ctgaccgacg
      481 aaattgcaga cggcatccat atttacacct gtttttcgcg ggcggatttg gaaaaagcct
      541 attccgaata tttggaactt ttcaaatccg aatcggatgc cgaaggcaga aagccgcagt
      601 aaggtgcaaa cagataccgt acacgttgag gagcagatat gatgggcgat tccgtcattt
      661 attatgtaga acaggcagac aaaccggtaa accgtgccgg cgaacgcgcc cgtaaaacat
      721 tcaaatattt ttggcgcgag cttttttggg aacgccgccg catcattccc gccttagatt
      781 ttgccatggt caaagcccct tttttccaag acggcgaaga cggcgaaatt tgcgaacata
      841 tgtggatcga cgatatttat tttgacggtc tttacattta tggcgtgctg aacaatgaac
      901 ccggcgaact gaccaatgtc gaacaaggcg aaagcgtttg cgttccggtt gacgacatca
      961 gcgactggat gttcgtgtgc aacggcattc cctacggcgg ctttaccata caggcaatgc
     1021 gtggacagat gacggaagag gagcgtaccg aacacgatgc cgcatgggga atcgatttcg
     1081 gcgatcccgg gcaggtattg ctggtgtatg aagaaaaaga acatcccgaa aatctggaag
     1141 agcatccgat gtgccggaac tgtattgacg attttcggca acagttgtcc caaaatccgg
     1201 attttctgca tgagcaggac gaagacggct atacgccgct tcatcatgaa gccatcgcag
     1261 gaaatgcact tatggttcaa gccatgcttg aatacggcgc aaatcctgcc tcaaagacat
     1321 cggaaggcta taccgccctc gattttgccc gcctggcggg ctggcaaaat gttgccgatc
     1381 tgctcgaacc gcgacattag gcggacagtt ttccgaaaac gaacacaaac actttttaca
     1441 gaaagacaat aaaaatgccc aaaatcaccg tacttccaca cacgacatta tgccccgaag
     1501 gcgcggttat cgaagacgcg cccgaaggca aaaccgtcct tgacgtgctg ctcgaccatg
     1561 atatcgaagt cgatcacgcc tgcgaaaaat cctgcgcctg cacaacctgc cacgtgatta
     1621 tccgcaaagg tttcgacagc ctggaagagc cgaccgaatt ggaagaagac ctgctcgatc
     1681 aggcttgggg tttggaagcc gattcgcgcc tgagttgtca ggcggttgtc gccggcgagg
     1741 atttgattgt ggaaatcccc aaatacacca tcaaccacgc gcgagaagaa cactgaaaac
     1801 aggccgtctg aagccggcac gcttcagacg gcattgttgc gcggataagg cgcaatcgcc
     1861 cgaaaacagg cgttcgtaca ggcggaactt tcgattctat agtgaattaa caaaaatcag
     1921 gacaaggcgg cgagccgcag acagtaccaa tagtacggca aggcgaggca acgctgtacc
     1981 ggtttaaatt taattcacta tatattgatt tttatcggtt ttctgacgga acggattggt
     2041 gcggcatccg aggcggatta ctcgggcgcg atgcacgggt atttatcggt tttgcagccg
     2101 gaaaaaccgc cggcgggtta ctttcagggc ggcggtcggg cggtatgccg gatagcgttc
     2161 cactttcgct tctatattga ttttgattgg ttttctgacg gaatgatccg atgcggcatc
     2221 cgggacggct tgtgtttctt cctgcccgcc tgccggatat tcctgtactt gcacgaaacc
     2281 gaaagagacg gcggcgcggc ggacaagctc gagatagcgt ccttcaagct ccaggcaggc
     2341 ggcggacacg ctttctaccg taaccgtgaa accgccgccc gacccggcaa ggcgttccgc
     2401 aatctgcgtg tagatgagcc aacgttgggc gcgcaacatt gccgcatcgc aaccggcagc
     2461 cgctttatcc aaggcaatca ggccgcgccg gttttggtgg tgaaggcaaa ccgttactct
     2521 ggagaggata tgttgcccgt ccaatatttg atacagtttg cgtatcagca gaatcaggcg
     2581 gtcaaactcc tccatctccg acagggaatc aggatggtcg gaagcggtat aaaccagttt
     2641 ggacaatttt gcggcaaaca tattgttcat caatcttcct tgtcggttga aaccccgctc
     2701 ttcggggcgg tagaatcaga tttgtttggg agggacaact cctcccagat caggacgaca
     2761 cataggctgg tgcttgatgt gttgtccggc gagttgaaac attcagccat cctcaaggtg
     2821 cggcagtttt gccgaaacat attctacacg gcttcaatgc cggacgataa aaggaaattc
     2881 atatgaaatg gaccgacacc cggcgtatcg ccgaagaact ctatgacctg cacggcgaaa
     2941 ccatcgatcc cagaaccgtg cgctttaccc aactgcgcga cctgattatg gcattgcccg
     3001 aatttgatga cgatcccgcc cgttgcggcg aacgtattct cgaagccgtg cagcaggcat
     3061 ggatagacga ggcagaataa gtttcgggaa tgccgtctga aatacggcgg tacgcggttc
     3121 gtgcttctgt ttgcagtggg aacggtttta ccagtctcct tttttcagcc tatccagttg
     3181 gcggcggtcg cgcttggtcg gtctgccgtc gggatgggcg gaagtgatgc ggctgaattg
     3241 gtcgagctgt ttgcgctctt ccctcaatgc tgccgttttt gcgtcttctt catacagaag
     3301 ccgcgcctcg gatgccgggc ggcgttggtg gttcaaacct ttaaccttga ttttataggg
     3361 aagggaattg agcgtcaggt cgataatatc gccgatgtct atggttttac tgtttttgac
     3421 cttcgagccg tttacttgaa ccctacccag ttcgatgtgc ttttgcgcaa gggaacgggt
     3481 cttgaaaaaa cgtgccgccc aaagccattt gtccagccgc atggcggaag aatcgtgctt
     3541 gtctttcata cgattttgtt tgaaataatt gaatttgttt cgagtttagc ataagatacg
     3601 ccgcctataa ctagtatata tgcactaatc cactgttttc catgctgtcc gaacacaaaa
     3661 agagggtgtg gaaaagccgt tttggacaat aaattaactg cggaatatgc acaaatagcg
     3721 tatgatagcg gcagaatctg ttgatgagag cttcattcta tgaaacctgt ttttttggat
     3781 tttgaacaac ccatagccga actgaccaac aaaatcgatg agctgcgttt cgtccaagac
     3841 gagtctgccg tcgatatttc ggacgaaata caccgtttgc agaaaaaaag caacgacctg
     3901 accaaatcga ttttcagcaa actcacaccc gcccaaattt cacaggtttc ccggcatccg
     3961 cagcgtccct atactttgga ttacattgag gcactgttta ccgattttga agaactgcac
     4021 ggcgaccgcc actttgccga cgattatgcg attgtcggcg gattggcgcg tttcaacgga
     4081 caaagcgtga tggtcgtcgg gcatcagaaa gggcgcgaca ccaaagaaaa aatccgccgc
     4141 aacttcggta tgccccgtcc tgaaggctac cgcaaagccc tgcgcctgat gaagacggca
     4201 gaaaaattcg gcttgcccgt catgaccttt atcgatacgc cgggcgcgta tcccggcatc
     4261 ggcgcggaag aacgcggaca gtcggaagcc atcggcaaaa acctgtacga actgacgcgc
     4321 ctgcacgtcc ccgttttgtg taccgtcatc ggcgaagggg gctcgggcgg tgcgttggcg
     4381 gtcgccgtag gcgattacgt caatatgctg caatattcga cctattctgt tatctccccc
     4441 gaaggctgcg cttcgatttt gtggaaaacc gccgaaaagg cggcggatgc ggctcaggct
     4501 ttgggcatta ctgctgaccg cctgcaaaag ctggacttgg tcgataccgt catcaaagaa
     4561 ccattgggcg gcgcgcatcg ggatttcggg caaaccatga aaaacgtaaa agccgttttg
     4621 gaaaaacaac tgcacgaagc gcaaagcatc ccgcttgccg atttgctttc gcgccgtttc
     4681 gaccgcatta tggcttacgg caaattttcg gaacaataat tcaggtagaa caagcagcaa
     4741 gcagtttgtc tgaaactgct tgctttttct ttatcgggac ggaaccgtgc tgactttaga
     4801 tgcgtttgag caatgcttga aggattgttt tcctcaaggt ctgaatggaa aaaaaacagc
     4861 ggtggcatta agcggcggct tggattccgt cgttttgctg catctgcttg tccgcgccgg
     4921 gaaaaaaggc ggttttattc cggatgcatt gcatatccat cacggcttga gtccccgtgc
     4981 cgacgattgg gcggatttct gccaaaacta ttgcgatatg ctcggggtgg ggctggaaac
     5041 ggttaagatc tgcgtggaaa aaaacggttt gggcatcgag gcggcggcgc ggcaaaagcg
     5101 ttatgccgcg tttgccgaaa aaggctttga cgttttggcg ttggcgcacc acagggacga
     5161 tcaaatcgaa acctttatgc tggcggtcgc gcgcggcggc ggtttgcgcg ctttggcggc
     5221 tatgcccgcc gtccgccctt ttggggaaaa aggcatcatt tggcggccct tgctgccttt
     5281 ttcgcgccaa gatatatggg attatgccca aaaacacggt ttgccgaata tcgaggacga
     5341 aagcaatagc gatacggctt atttgcgaaa ccgcttccgg caccgtattt tgcccgaact
     5401 ttcggcgcag attccccatt tcgggcggca tctgttgaac aatgtccgtg ccttgcagga
     5461 ggatttggct ttgttggacg aggtcgtcgt tcaagactgc cgttgggttt gcggagccgg
     5521 ttatttcgat acggcgcggt ggctgacgtt ttccccgcgc cggaaaaccc atattttgcg
     5581 gcattttttg aaggaaaacg gcattcccgt gccgaatcag aatgcccttg ccgacattgc
     5641 ccgggttttg acggaggcaa aaaccggacg ttggaacgtg caaggctttg aattgcatca
     5701 ttatgcaggc aggctgtttg tgttccgacc ggaaaaaacg gataaactgc ggtttttgaa
     5761 agacaggcag ataagcggaa atttaaggga aatattggcg gggcagggat ttgttttaaa
     5821 acgtcatccg ttcggacttc ccgggcatct tttggagcag gacggaatct tgcggacggt
     5881 tgcggcatcg gacaggctgg ctgtcggcgg tatccataag gatgtgaaga aaatccttca
     5941 ggaaaagcgg gttttgcccg cgctgcgccc gttttggccg gtcgtcgccg acaaggacaa
     6001 ccgtccgctg gcgttggcaa attacggtac ggattttcaa atctcggttt cagacggcat
     6061 tttgcccgtc catccggatt ttcccgtttt attttgatgg aatcggaatc gccgttacct
     6121 attatatatt cttgaaaagg cggtaaagtg aagcatatca gttcggcaaa caatgaacac
     6181 atcagacacc tgcaccgcct gttgtctcaa gggaagttca gacggcaata cgcccaaacc
     6241 gttttggagg gcgtgcacct gcttcaggtt ttcctgcaat ccggcggaag gccagtcggg
     6301 gtatatattc ccgaagcgaa aatgccgtct gaagaagtcc gtaaattgac ggcggttttg
     6361 ccggaagacg gggttttttc cgtttcagac ggcatattga agaaaatcag cagcctgact
     6421 tgcgcggatg atgtgcttgc gctgattgat attccggatg cgggtgcttt gccggccggc
     6481 ggcgactgtg tggttttgga cggcgtgcaa gatccgggca atgtcggcac ggtgttgcgg
     6541 agcgcggcgg cggcaggaat cggcgcggtc gttttgggca aaggttgtgc ggacgcgtgg
     6601 tcgcccaaag tgctgcgagc cggaatgggc gcgcatttct tgtcggagat ttatccgcag
     6661 gcggatttgg aaatatggtt ggcgcgttat gaagaccgtg tgtttgccac cgcgttgcgt
     6721 gaggaaaaac aggcggttct gtataacgaa gatttgcgcg aaccgacagc ctgggtgttt
     6781 ggcaacgaag gcgcgggggt cggtaaagca gttttagaca gggcggacaa gtgtgtcagg
     6841 ataccgatgc acgatgcaac cgagtcttta aatgtcgcga tggcggcgac aatctgcctg
     6901 tttgaacaaa tgcgccaacg ggcggcgtat tgaggaaaag aaatgccgtc tgaaaaaatc
     6961 tattacggcg tattgatttt cttatgtatc gcttctatgc tgctgtcgcc gtttttttat
     7021 gcgggtgctt tgaagcccaa gaaggcggca ttgcggaagg acgggcagtg gaaactcatc
     7081 ttattgtcca ataccgtggc ggcggcggtt ttggcttggg tgtggtggaa atggttttga
     7141 cagatattgc tcaaaatcgt gctaatggaa tccgaacaaa taaagaattt ggtaaaaaat
     7201 ttgttaaatc aacgaattaa agttttgtgg aaaacaaaac agctctaagc aaatagggcg
     7261 tttgtcggta aatacggaag agttgcggca ttatcgggca tctttaacaa gtagtgccgt
     7321 cttgacaggc aatcggtttt tatgggcagc ttgcaaaatc gcggatataa aattgcaaat
     7381 cggttaaagt gtggggacgc tatgaaaaat tgcgaatttt tttatgaccc gacaagggca
     7441 atctatgata gcggtgcaga ttatttaacc agagaaaaac atagattcgt cgtaattgca
     7501 aatagtgctt gggggctatt gcttaattta tcttgttatt atgacgaggt tttggaaaag
     7561 cggaaaatac cgttcggcaa acaggaaatt gatgacgata tggacaaagt gtccgcccat
     7621 aagcggaagt ttaaagatat ttctgaaatc aaagtagggg atggttggga atacccgttc
     7681 aattatgagc agggaatgaa agaattagat gaagtgctat tgaaatacat tccctttttt
     7741 gaagaagaac gataaaggag attgatatgc gcgtatctaa aataattgga agtatgttgc
     7801 ttgttacagc ggttcagacc gtattttcgg caaatgttta cgcgtgccgc cataatggta
     7861 aaaccagtta cagccaaact ccgggaaaac attgtaccaa cgcgggtttg gggcgggatc
     7921 gggtgtacag ttcggttaga ccggcagtaa aagacagggc ggaagacgca ggggtcggcg
     7981 attattcgga cacggtgagg gacgaacacg tccaaaatcc gagagagaat acgcataagg
     8041 atgcttcgaa taccggtgca aagacgcatt gattgaaacc gaatcagcca tgacggcgtt
     8101 gaataataga atttgaacga ttagggaact ctgatgaaac acatccacat tatcggtatc
     8161 ggcggcacgt ttatgggtgg gattgccgcc attgccaaag aagcagggtt tgaagtcagc
     8221 ggttgcgatg cgaagatgta tccgccgatg agcacccagc tcgaagcctt gggcataggc
     8281 gtacacgaag gcttcgacgc ggcgcagttg gaggaattta aagccgacgt ttacgtcatc
     8341 ggcaatgtcg ccaagcgcgg gatggatgtg gttgaggcga ttttgaaccg cgggctgcct
     8401 tatatttccg gtccgcaatg gctggctgaa aacgtgctgc accatcattg ggtactcggt
     8461 gtggcgggga cgcacggtaa aacgaccacc gcgtccatgc tcgcatgggt tttggaatat
     8521 gccggactcg cgccgggctt cctcatcggc ggcgtaccgg aaaacttcag cgtttccgcc
     8581 cgcctgccgc aaacgccgcg ccaagacccg aacagccaat ctccgttttt cgttatcgaa
     8641 gccgacgaat atgacaccgc gtttttcgac aaacgctcca aattcgtgca ttaccgtccg
     8701 cgtaccgccg tgttgaacaa tctagaattc gaccacgccg acatctttgc cgatttgggc
     8761 gcgatacaga cccagttcca ccacctcgtg cgcaccgtgc cgtctgaagg cttaatcatc
     8821 tgtaacggac agcagcaaag cttgcaggat actttggaca aaggctgctg gacgccggtg
     8881 gaaaaattcg gcaccgaaca cggctggcag gtcggcgaag tcaatgccga cggctcgttc
     8941 gacgtgttgc ttgacggcaa aacggccgga cgcgtcaaat gggatttgat gggcaggcac
     9001 aaccgcatga acgcgctcgc cgtcattgcc gccgcgcgtc atgtcggtgt cgatattcag
     9061 accgcctgcg aagccttggg cgcgtttaaa aacgtcaaac gccgcatgga aatcaaaggc
     9121 acggcaaacg gtatcaccgt ttacgacgac ttcgcccacc atccgaccgc tatcgaaacc
     9181 acgattcaag gtttgcgcca gcgcgtcggc ggcgcgcgca tcctcgccgt cctcgaaccg
     9241 cgttccaata cgatgaagct gggtacgatg aaagccgccc tgcccgcaag cctcaaagaa
     9301 gccgaccaag tgttctgcta cgccggcggc gcggactggg acgttgccga agccctcgcg
     9361 cctttgggcg gcaggctgca cgtcggcaaa gacttcgatg ccttcgttgc cgaaatcgtg
     9421 aaaaacgccg aagcaggcga ccatattttg gtgatgagca acggcggttt cggcggaata
     9481 cacaccaaac tgctggacgc tttgagatag cccgggcgat gccgtctgaa agtccttcag
     9541 acggcatcgc ccggctgcgc ggcacaaagg cggaaaaacc gtttgccccg tattttcaaa
     9601 cgcgttacac ttgccgccgc tgttttcagc catttgatta cccgcaaccg ccgtcattgc
     9661 gccggcggtc tgcacgtcag cgtcattgcg ccgctgtaaa tacgaaagaa cacattatga
     9721 ccgtatcccc cgtcgccttg cgccgcaaga ccgagtgcaa gccgcatccc accgcgcgct
     9781 attggaaaaa atgcgatgtc gaagccctgt tcggacttcc cttcctcgac ctcatttacc
     9841 aagccgctga aatccaccgc caaaatttca acccgcgcga aatccagctt tccacgctgt
     9901 tgtccatcaa aaccggcggt tgtcccgaag attgcgccta ctgtccgcaa tcggcgcacc
     9961 acaacaccaa tctgggcaaa gagcagatga tggatgtgga tgaaatcgtc gaaaaagcca
    10021 aaatcgccaa atcgcgcggc gcaagccggt tttgtatggg cgcggcatgg cgcggcccca
    10081 aacccaaaga cgtggagacg gtttccgcaa tcatcaaagc cgtcaagggc ttgggtatgg
    10141 aaacctgcgg cacgttcggt atgctcgaag aaggtatggc ggaagacttg aaagaggcgg
    10201 gcttggatta ttacaaccac aacctcgaca ccgaccccga ccgctacaac gacatcatcc
    10261 atacacgccg gcatgaagac cgtatggaca ccttgggcaa agtccgcaac gccggtttga
    10321 aagtctgctg cggcggcatc gtcgggatga acgaaactcg tgccgaacgc gccgggctga
    10381 ttgccagcct cgccaacctc gacccgcagc ccgaaagcgt gccgattaac cggttggtca
    10441 aagtggaagg cacgccgctt gacgatgccg aagatttgga ctggacggaa tttgttcgca
    10501 ccatcgccgt ggcgcggatt acgatgccgc aaagttatgt ccggctgtcg gcagggcgca
    10561 gcaatatgcc ggaagcgatg caggcgatgt gctttatggc gggcgcgaat tcgatttttt
    10621 acggcgacaa gctgttgacc acaggcaatc cggacgaaga cggcgacaga atcctgatgg
    10681 aaaagcttaa cctgtatccc ttacagtttg agctggaagg cgaggtcgcc gaagtggaaa
    10741 aagcctcggg gattaaagtg gattattgac gattgaaaaa tgccgtctga aacccggaaa
    10801 aaggctttca gacggcattt gtccggacgg catttccaat atctttttac cggcgcgtga
    10861 tgctgccgtc gggcgagaca tccagtccgt tcccgttggg gaagtcgctg ctgttcagat
    10921 aaataatccg cccgataacg gtttttcagg gttgattttg gggattttat cgtcggcttg
    10981 ggtaatcggg atgatggttc cggaaataaa tttaccctgt ttgtcggtga taattttaaa
    11041 aatgggggcg atgccgctga tgccggaggt gttgattgcg ccgtaggtgg caaagttgcc
    11101 gccgctgtag gagatgaagc ggtcgcggta aagttcgacg gcgcgagtaa cgtgcggcct
    11161 ctgcccgaat acgacatccg cgccggaatc gacggcaagc cgcgcaaact caacgacgtt
    11221 gcccctgttt tccccataga agatttcggt atcgaacggc aggtgttccg cctgtttccc
    11281 ttccgcgccg ccgtggaaca tcacaatgac gatgtcggct ttctgtttgg ttttggtaat
    11341 ccgttttcta actttggcat aatcgttcag tttgacggcg gcaaggttgg gggcgaagga
    11401 gacgaagccg gatcttacgc cgtttttctt caggatggcg gtttcaaacc tgttttcgat
    11461 gcccgaatat ttgatgttca attcgtcaag gttcgccctt tatgccgttt ccgcaccgca
    11521 aaccgccggg gtcaatccct cggcgcatcc tgccggaacg gaatccccgt gctgccgatt
    11581 gacggttcaa accccgcgcc cgtttcaaat accggcgatg tggacggaca ggatgcgcct
    11641 gacgaaaaga cagccgatac cgtttccatt atcggcgtgg gcgacattat gctcggcagc
    11701 aattatccgg tcgattacct gcccgatacc aatattctga aaaacgtcga atctgccttg
    11761 caagacgcgg acattaccgt cggcaacctc gaaggcacgc tgtttgacga aggcggtacg
    11821 ccgaaaaaat gtgcaacccc cccaaaatat gctatgcatt ccgaacgccc tccgcatacg
    11881 ggcaatacct tgccgacgcg ggattcgact acctcagctt cgccaacaac cacagcaacg
    11941 acttcggcgc gcaaggcatc acggcaacgg cggcgagcgg cagctctttt acatactcga
    12001 tcgcgctaaa gccgctgccg ataacgaggc caaaattgcg gaaaataccg ccatcgccca
    12061 gataaatttg tccatcatca gacctttact gttcagacga gacagcattt gccgcacgtt
    12121 ttggggctta tctttcgatt tgcgctacgt cgcgcaccgc gcctttgtcg gcggaagtcg
    12181 ccatcgcgcc gtaagctctt aatgcggcgg agacgtagcg gtcgcggttt ttaggcttcc
    12241 atgctttgct gccgcgcgct tccatttcgg cacggcgtgc ggcaagctct tcatcggaaa
    12301 tggcaaggtg gatgctgcgg ttggggatgt cgatttcgac ggtatcgcct tcgtgtacca
    12361 aaccgatcgc gccaccttcc gccgcttcgg gcgaggcgtg tccgatggac aaacctgatg
    12421 tgccgccgga gaagcgtccg tcggttaaga gagcgcaggc tttgccgagg cctttagatt
    12481 tcaggtagga agtcggatac agcatttcct gcatgcccgg gccgcctttc gggccttcgt
    12541 agcggatgat gacgatgtcg ccagcgacga tttggttgcc caaaatgcct tctactgcgt
    12601 cttcttggct ttcaaacacg cgggcgcggc cggtgaattt gaggatgctc tcgtccacgc
    12661 ctgcggtttt taccacgcag ccgcgctcgg cgatgttgcc gaacaagacc gccaaaccgc
    12721 cgtcttgcga gtaggcgtgt gccacgtcgc ggatacagcc tttttcgcgg tcgaggtcga
    12781 gggttttcca catacggttt tgcgagaacg cttgggtggt gcgtacgccg ccgggcgcgg
    12841 ctttgaagcg ttcgatggcg cgggtgtttt cagggttggt tatgtcccat ttttcaatcg
    12901 cgtctttcag cgtcggtgcg tggatagtgt gcacgtcggt gtgcagcttg cccgctttgt
    12961 ccaattcttt caggatggcg aaaatgccgc cggcgcggtg cacgtcttcc atatagtagt
    13021 cgtggttgtt gggtgcggtt ttgcagatgc agggcacaac gcggcttaag cggtcaatgt
    13081 cggccatttt gaaatcgaca ccggcttcgt tggcaacggc caacaggtgc aaaatggtat
    13141 tggtgctgcc gcccatcgca atatccatcg tcatggcgtt ttcaaacgct tttttggtgg
    13201 caatgctgcg cggcagcacg gtttcgtcgt tttgttcgta atagcgtttg gtgatttcga
    13261 caatcatacg gccggcttcg aggaacagtt ctttgcggcc ggcgtgggtg gcgaggtaag
    13321 aaccgttgcc cggcagggac agcccgagtg cttcggtcag gcagttcatc gagtttgccg
    13381 taaacatacc cgaacacgag ccgcaggtcg ggcaggcgtt ttgttcgact tcttcgactt
    13441 gccggttgct gacattgtcg tctgccgatt cgatcatcgc gtcaatcaag tccaagcggc
    13501 gttctggttg gatgtttgcc acgccgataa ctttacctgc ttccatcggg ccgccggaga
    13561 cgaagatggt ggggatgttc aggcgcatcg cggcaatcag catgcccggg gtgattttgt
    13621 cgcagttgga aatgcacacc agcgcgtcgg cgcagtgggc attgaccata tattcgatgg
    13681 agtcggcaat caaatcgcgg ctgggcaggg aatacagcat accgctgtgt cccatagcga
    13741 tgccgtcgtc gatggcgatg gtgttgaatt ctttggcgat ggcgccggct ttttcgattt
    13801 ctccggcaac cagctggccc atattgtgca ggtggacatg acctggcacg aattgggtga
    13861 acgagttggc aacggcgata atcggtttgc cgaagtcggt ttccatcacg ccggtggcgc
    13921 gccacaacgc gcgcgcgccc gccatattgc gaccgtgggt ggaggttttg gagcggtatt
    13981 ctggcatagt gtgtttcctt gtgcctatac cgtctgaaag acagggctgt ttcagacggt
    14041 atcgggtacg gttttttaga gtgggaaaag agggtatttt ataccaagta tcggaatttt
    14101 gcgggattga aacggcgtgc ggcaaaaaag aaaatccccg caggaatgcg gggacgggtt
    14161 caggcgcggg caatcgcgac ggctttggat gcgtcccaaa aatcaacggg tgcatttaat
    14221 acgggtttga cgatgcccgt ccgtatggag aaatcgccga aaccttcgcc gatattgcgt
    14281 tctgccgccc atttgccgat caggtcgtcc aattcggcaa ggatttcggg cagggtgatg
    14341 ttttctttgt aaagacgggg gatgcgtacg ccttcacggt cgccgccgat atggaggttg
    14401 tagcgtccga cggctttgcc gaccagtccg atttccgcca acatcgcccg tccgcagccg
    14461 ttcgggcagc cggtaatgcg ggtaacgatg tagtcgtccg acgtgccgtg tttcgccata
    14521 atcttatcca gctcgccgat gaagtccggc agcacgcgtt cggcttccgc cattgccagc
    14581 gggcaggtcg ggaaggaaac gcacgacatc gcattttcgc gcagtttgct gacaccgtcg
    14641 tgtatcagcc cgtatgagcg ggcgagctgt tcgatccggg ctttgcctcc ttcgggtaca
    14701 tttgccacga tgaggttttg gttggcggtg atgcggaagt cgcctttgtg gattttggcg
    14761 atttccaaca cgccggtcag aagctgtttc ccgccttcgt caaccaaacg cccgctttcg
    14821 atgaaaaggg tcaaatgcca gttgccgtct atgcctttca cccagccgat acggtcgccg
    14881 cgcccggtaa atttgaacgg gcgtacgggt tcgaacggca tacccatacg gcgttcgact
    14941 tcggcgcgga agttgtccaa gcccatattt tgaatggtgt agcgggtgcg ggcgtttttg
    15001 cggtcgctgc ggttgccgaa gtcgcgctgc gtggttacca ccgcttcggc ggccttcagc
    15061 gcgtgttccg gaggcacgaa acccagttcc agtgaaatgt tcggataggt tttggtgttg
    15121 ccgtgttcca tcgaaagccc gccgcccgcc aaaacattga atccggcaag ctgtccgttg
    15181 ccgtctgaaa tggcgacaaa atccaagtcg ttgccgtagc agtccacatc atttaagggc
    15241 gggatgacga ctgcggtttt gaatttgcgc ggcagatagg ttttgcccaa aatcggctcg
    15301 tcttcttgaa ggaagtcgtc agaactttga acttttttgc cgtccaccca cacatccaga
    15361 taaccgcgcg tgcgcggcag caagtgttcg gaaatctttt tcgcgtattc gtaagcctgc
    15421 cggtgcagtt cggactcgat cgggttggac gtgcaaagca cgttgcggtt catatccgcc
    15481 gccgtggcga tggaatccaa acccagtttg tgcaagaggc ggtgcatcgt ctgcaacttg
    15541 gctttcggca cgccgtgaaa ttggaaggtt tgccggttgg tcagccggat ggagcggtaa
    15601 tgactgtttt cccgggcaaa tttgtccagt tctatccatt gggacggttt gatgatcccg
    15661 cccggcagcc ggcagcgcaa aagcataaat ttcaagggct cgagttttgc ctcggcgcgt
    15721 tcggcgcgga tgtcgcggtc gtcctgctca tacataccgt ggaagcggat gagttggaag
    15781 ttgtcgcctt tgaagccgcc cgtgagcggg tctttcaaat cgtccaaaat cgtgccgcgt
    15841 aaaaaattgc tttcggtttt cagacgttcg ttgtcggata gcggtttttc ttgccacgcc
    15901 aaaccttttg tcttggtctg tacggtcatt ttgtgttcct cccgattata tttaatcaat
    15961 aaacatcacg ctgatagcgt ttttcttcgc gcagcatatc caaatattct tctgcgccct
    16021 cttcgtccaa atgtcctgcc ccgataatca catccagcaa ggcggcctcc acgtcttttg
    16081 ccatttttgc cgcatcgccg cacacataga tatgcgcgcc ttcctgcagc cattgccaaa
    16141 gtccttccgc ctgttcgcgg attttgtcct gcacatagat tttttcttcc tgatcgcgcg
    16201 accaggcgaa atcgtatctg tgcaggaagc cgtctttggc aaactgctgc cattcggttt
    16261 gatagagaaa atcacgggca aaatgcggat tgccgaaaaa cagccagttt ttgccttccg
    16321 cattttctgc ggcacgttgt tggacgaaag cgcggaacgg tgcgacgccg gtgcccgagc
    16381 cgatcatcac aatcggcttg cggctgtctt cgggcagcct gaagccgtcg ttgcgttcca
    16441 caaacacgcg caccgtgccg tcctcttcca gccggtcggc aaggaaaccc gatgcgccgc
    16501 ccgccctggc gcgcccttcg tgttcgaaac gcaccgcgcc gacggtcagg tgcacttcgt
    16561 cccccacttc cgcctgcgac gaggaaatcg aatacaggcg cggcgcaagc gggcgcagta
    16621 ggccggcgaa ttgttccgcc gtcagttttg ccgggaagcg gtgcagcaca tcggcaatcg
    16681 gcgtgctttg cacaaagcct tgcaaaacgg cgttgtcggc agcaatacgg tcgagttcgt
    16741 catcatcggc gaacggggca tagcctttga caaaggcggg ggtgttttgc gtgagttcaa
    16801 aatgggataa cagtgcggag gcaaccggca gggtttttcc gcccgcctgt atttccgttg
    16861 cctgatcgat gccgagcagg tctaggattt ccctgaccag tgccggatcg ttgtcaaacc
    16921 aaacgccgag cgcgtcgccc gggaggtagt gcaaatccga accgctcaaa tcgatttcga
    16981 tgtggcgcac gtctttatcg gattggcggg cggtgatttt ctgattggcc agcagggcgg
    17041 cgggaaaggg gtctgccttg cagtacctgc catccggtgc cgtctgaagg ccggcggggg
    17101 gcgttgtctg cggcgcgggc gttgcccggt tttttgcggc ttcttctttt aagcgcgcga
    17161 caatgcggcc tgtccatccg tctgcggagg cggtaaagtc caaatccgca tcaacgcgtt
    17221 cgagcaggcg tttcgccccc aattcttcaa aacgccggtc gaaatcttta cctgcctgac
    17281 agaaattcgg ataggagctg tcgcccaagc ccagtacggc aaattggagt ttgtccaatt
    17341 tcggggcttt tttgccgttc agcagtttgt gcagcacgac ggcttcttcc ggcggttcgc
    17401 cttcgccttg ggtggaggta accagcagca ggcggcgttc gccggcgatg tttttcgcct
    17461 tatagtcttt cagttcggcg cgactgactt ggatgccggc ggcttccagg ctgtccgccg
    17521 ctttgtcggc aacggatttc gcattgccgg tttgcgaggc ggaaaggacg gttacggaaa
    17581 aaggttctgc cgccggcaat gccgtctgaa gcgcgggcag tcctgcagat gccccgtttc
    17641 ctgcttttgc ccaagcgtag ccggacagcc acgcccattg tgccgcgtcc agcaccgaca
    17701 ggagctgcgt gatttcgggc ggcagaggcg gtaatggcgg atttgtgttc tgcatatcgt
    17761 gttcactcat aaaatcatac ctgccgcaac agtgccgtat gtcgcttcgt ctatcaggat
    17821 aaacgaaccg gcggcagtgt tttccgcata aggcgttgcc gtaacgggtt tttgaaggtt
    17881 gatgcggact ttggcgatgt cgttcatctt caaggattcc gcgccggcct cttgttccag
    17941 cgtgcggaca tccaaaacgc tttcaatttc cccgactttt gccgacacgg tttgcgtgcc
    18001 gtgcttgagc aggtatttgc gcgcggtgtt gagcggacgt tcgtcaaacc agcaaagcgt
    18061 ggcttccaga tgtttttgcg gggcgagcgg ggaattttta tcgacaaaaa ggtcgccgcg
    18121 cgaaacatcg atgtcgcgat ccagccggag ggttgccgcc tcgccggcaa aagcctgcgc
    18181 cacttcccct ttcggcgtga tgatttcgga cacttcggcg gtcagcccgt tcggttcgat
    18241 gcggacggtt tgcccgacgg cgaccgaacc gcgttcgatg cgcccctgat agcctcggaa
    18301 atcatcggcc ttgtcggcat cttggcggac gaccagttgc acggggaaat aaaaatcgtc
    18361 ggcggtgcgg ctgacttcgt ccgcccccgg cagggtttcc aaaatggaca ataagggttc
    18421 gcctttatac caaggcatat tgccgccggg gtaaacaatg ttgtcgccca agagtgcgga
    18481 caccggtacg aaatgcgcgt ctttcaaacc gagctgttcg gcaagtcggc ggtatgcctc
    18541 cacaatggcg ttgaatttgt cttcgctgta atccagcagg tccattttgt tgaccgccac
    18601 cacaatatgc gggcagttga gttggcggag gatggcggaa tggcgtttgg tctgcggcag
    18661 aagctgcaag ggctgcgcgc cgaaatccag ttgggatgcg tcaaccagca cgactgccgc
    18721 cgaagcggtg cttgcgcccg taaccatatt gcgcgtgtat tgttcgtgcc ccggcgtgtc
    18781 ggcgatgatg aatttccgtt tcgccgtgga aaaatagcgg tatgccacat cgatcgtaat
    18841 gccctgttcg cgttcggctt ccagtccgtc ggtcaggatg gagaagtcta tggcttcttt
    18901 caaacctttg cttttgccgg attccaaggt tttgatttgg tcggacagca gggctttgct
    18961 gtcgtagagc agccgtccga tcagggtgct tttgccgtca tcgacgctgc cggcggtaat
    19021 gaagcggagc ggggcctggt gttgtgccgt catattttct tcctcatatc tgcttaaagg
    19081 gtttttgaaa tttagaaata gccttctttt ttgcgttttt ccattgccgc ctcgcttgcc
    19141 tgatcgtcca gccgggtcgc gctgcgttcg gaaatgtcgg taactgccgt ttccctgata
    19201 atctccgtcg gcgtggacgc ggtgctttct accgggcagg tgcagctgat gtcgccgacg
    19261 gtgcggaagc ggacatcaag gatttcggag gtttcagacg gcattttcgg cgtaagcggc
    19321 gtaacgggga ccagcagccc cctgcgtctg accacttcgc gcctgtggct gtaataaatc
    19381 ggcggcagct cgaggttttc gcgggcgatg tattgccaga tgtcgagttc cgtccagttg
    19441 gaaatcggga agacgcgcat attttcgcct ttgtgcagcc tggtgttgta cagcgaccac
    19501 agctcggggc gttgcgcctt cggatcccat tgtccgaact cgtcgcggaa cgagaaaatc
    19561 cgttctttgg cgcgggcttt ttcttcgtcg cgccgcgcgc cgcccatcag cgcgtcaaag
    19621 ccgtttgcct cgatggtttc caacagcgta accgcctgcg ccgcattgcg cgaatcggtt
    19681 tctttgcgta aaactactgt gcctttagcg atggagtctt ccacgcgccc cactatcagg
    19741 cgggcattga gttttgccgc ctgcgcgtcg cggaaggcaa tcacttcggg gtagttgtgc
    19801 cccgtgtcga tatgcaccag cgggaagggc agttttaccg gcctgctgcc cagccggaag
    19861 gctttgcagg cgagggcgag caggaccacg gaatctttgc cgccggaaaa gagcagggcg
    19921 gggttttcgc attctgccgc cacttcgcgg ataatgtgga tggattcgga ttccaaccag
    19981 tcgagttgga cgttgttcgg ttcggttttc gtcatacgat attccttatt tcttctgttt
    20041 gatccttatg aattatttgt gcagcccgca ttctttgctg tttttatctt cccaccacca
    20101 ccgcccggcg cggatgtctt cgccggcctt gacggggcgg gtgcaggggt cgcagcctat
    20161 actgggaaag ccttgccggt acaaatcgtt gtaaggcaca ttgttggcaa ggatgtatgc
    20221 ccacacgtcg tgttccgacc agtcgaaaat cgggttgtat ttatcgatgc cgcgcccggc
    20281 atcatattcg gcaaacggca gttccgtgcg tgtggcggac tgttcgcggc gttgcccggt
    20341 aagccaagca tccgccccgg caatggcgcg gtcgagcggt tcggtttttc ggatgcggca
    20401 gcattcgcgg cgcgcttcaa cgctgtcgta aaaggcaaac ctgcctttgc tttccacata
    20461 atgcagggca tcttcccgta tcggttggaa acgcttgatt tgcatatgcg gataaacacg
    20521 ttcaatccta tccaacaggt ttaaagtttc cgcgtgcagc agccccgtat ccaaagtaaa
    20581 aataccgata ttgaggtttt ctccggcgat aaggtcggta attaccatat cttccgcagc
    20641 aaggctgctg gcaaaccgtg catcccggtg gctgccggca atccggtgca ggcgttgttt
    20701 gagggtttcc gttttttccg ccaaagccgt ttcactgcca ttttcaatcg gcggaatttt
    20761 ccataaagca ggtttgaaaa ttttcatttt gtccgccttg ctttatgcgg cttgttgttc
    20821 ttgtccggtg cccagtccgt ccaatgcaaa atacgcgtgt tcggccggat tgctttcttt
    20881 tttggcgttt tcgccgaacc aagccagttt ttcgtgcagc cccaccactt cgcctatgac
    20941 aatcaatgcc ggattcggcg cggtttcggc gagttcggca aggttggcga gcgtgccggt
    21001 tgcggttttt tgagccggca gcgtgccttg gctgataacg gctgccggcg tgtcgggcga
    21061 gcgtccgtgc tgttgcagcc gttcggcaat cagggcggct ttgagcgcac ccatataaat
    21121 caccaaggtc tggcggctgc gggcgagggt ctgccattcg atgtcgggcg catccgcctt
    21181 gcggtggccg gttacgaaaa ccgcactttg ggcataatcg cggtgcgtga gcgggatgcc
    21241 ggcataggcg gtcgcgccga cggcggcggt aatgccgggg acgaccgaaa acggaatctg
    21301 atggcgtgcc aaggtttcca attcttcgcc gccgcgtccg aacacgaaag ggtctccgcc
    21361 ttttaaacgc accacgcgcc tgccttcgcg ggccagcctg accataagcg cattggtgtc
    21421 ctcttgcggg gtgcggccgc cccgggcgcg cttgccgaca aaaatccgtt ccgcatcgcg
    21481 gcggacgagg gacagtatgc cgtctgaaac cagcgcgtcg taaagcacca cgtctgcctg
    21541 ctggatttcc tgcagccctt tgagcgtcag cagccccgca tcgccgggac ccgcaccgac
    21601 caaagacaca aaaccgcctt gataattttg ttccaactgc tttgccaact cctgttcggc
    21661 ttgggcggtt tgttgatttt taacgagggc ggcaaaacgg ccgttgaact gcttttccca
    21721 aaaacggcgg cgttcggtaa cggatttcag tttggctttg accgcatcgc gccatcttcc
    21781 ggagatttcc gccatatcgc ccaaagacgg cggcagcagg gcttccagtt tttcacgcag
    21841 caaacgggcg aggacgggcg cgctgccgga gctggaaacg gcaatttgaa ccgggttgcg
    21901 gtcgataacc gatgggaaga tgaagctgca atggtcgcgg tcgtccacca cgttgaccgg
    21961 cttttggcag ctttcggcaa gatggaaaac gcgccggttg agggcttggt cgctgcttgc
    22021 cgcaatgatg aggaaaaccg tgcggatgtg ttcggcacga aattcttcgg caagccacag
    22081 gattttgttt tccgccgcca acgcggagag ttcggtattc aggtgttttg cggcaaccct
    22141 gacctctgcg cccgccttca gcagcaggct gattttgcgt gcggcgaccg cgccgccgcc
    22201 tacgaccaat acggggcggc cggcgaggtt ggcgaaaatg gggaaataat tcactggctg
    22261 actcctttgc tgtttgcccg caccttgttt ccgatacggt gcgtcgtggc atttttgtcg
    22321 gaatgcgggt cattttagac aaaaggattt tcccggttaa ataataaaaa ggcatttgtt
    22381 agaagctgaa agctatatga gggcggctgc ggatgcggcg gttt
//
LOCUS       DAXHSJ010000015        22369 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000015 DAXHSJ010000000
VERSION     DAXHSJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.015"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            1..99
                     /locus_tag="ACFM8U_000426"
                     /pseudo
     CDS             1..99
                     /locus_tag="ACFM8U_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(120..1034)
                     /locus_tag="ACFM8U_000427"
     CDS             complement(120..1034)
                     /locus_tag="ACFM8U_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6333719.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEYFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="ACFM8U_000428"
     CDS             1290..2759
                     /locus_tag="ACFM8U_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HFC6333720.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFATPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="ACFM8U_000429"
     CDS             3011..3382
                     /locus_tag="ACFM8U_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333721.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLDSSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /gene="thrC"
                     /locus_tag="ACFM8U_000430"
     CDS             3455..4864
                     /gene="thrC"
                     /locus_tag="ACFM8U_000430"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC6333722.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="ACFM8U_000431"
     CDS             4910..5704
                     /locus_tag="ACFM8U_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333723.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAITTESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLAGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSEQP
                     AEIQRLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADIPP"
     gene            5892..6668
                     /locus_tag="ACFM8U_000432"
     CDS             5892..6668
                     /locus_tag="ACFM8U_000432"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6333724.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6744..6850
                     /locus_tag="ACFM8U_000433"
                     /pseudo
     CDS             6744..6850
                     /locus_tag="ACFM8U_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7429..9900)
                     /locus_tag="ACFM8U_000434"
     CDS             complement(7429..9900)
                     /locus_tag="ACFM8U_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6333725.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPIDGTVLEGASTVDESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGNPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILKHRVSDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9897..11075)
                     /gene="hflX"
                     /locus_tag="ACFM8U_000435"
     CDS             complement(9897..11075)
                     /gene="hflX"
                     /locus_tag="ACFM8U_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC6333726.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSTARLNGFQTAL
                     AEAVELVKAAGGNPVLVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11080..12360)
                     /locus_tag="ACFM8U_000436"
     CDS             complement(11080..12360)
                     /locus_tag="ACFM8U_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC6333727.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            12362..12490
                     /locus_tag="ACFM8U_000437"
     CDS             12362..12490
                     /locus_tag="ACFM8U_000437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333728.1"
                     /translation="MPSSRRGNGVFYQNGKLANAVSDCRLPNRQTFPRADAEPDAV"
     gene            complement(12513..13238)
                     /locus_tag="ACFM8U_000438"
     CDS             complement(12513..13238)
                     /locus_tag="ACFM8U_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC6333729.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMNQINDCGRRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13311..13988)
                     /gene="radC"
                     /locus_tag="ACFM8U_000439"
     CDS             complement(13311..13988)
                     /gene="radC"
                     /locus_tag="ACFM8U_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC6333730.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGTGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVKELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            14015..14137
                     /locus_tag="ACFM8U_000440"
     CDS             14015..14137
                     /locus_tag="ACFM8U_000440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333731.1"
                     /translation="MPSETDVPTRQISEMPSEPDFRFRRHLYFKTVLRRFFQLD"
     gene            complement(14111..15460)
                     /gene="gshA"
                     /locus_tag="ACFM8U_000441"
     CDS             complement(14111..15460)
                     /gene="gshA"
                     /locus_tag="ACFM8U_000441"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC6333732.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15720..17129
                     /gene="leuC"
                     /locus_tag="ACFM8U_000442"
     CDS             15720..17129
                     /gene="leuC"
                     /locus_tag="ACFM8U_000442"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC6333733.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRGLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17226..17480
                     /locus_tag="ACFM8U_000443"
     CDS             17226..17480
                     /locus_tag="ACFM8U_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333734.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17542..18183
                     /gene="leuD"
                     /locus_tag="ACFM8U_000444"
     CDS             17542..18183
                     /gene="leuD"
                     /locus_tag="ACFM8U_000444"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC6333735.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18357..19627
                     /locus_tag="ACFM8U_000445"
                     /pseudo
     CDS             18357..19627
                     /locus_tag="ACFM8U_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            19643..20374
                     /locus_tag="ACFM8U_000446"
                     /pseudo
     CDS             19643..20374
                     /locus_tag="ACFM8U_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
     gene            20408..21478
                     /gene="leuB"
                     /locus_tag="ACFM8U_000447"
     CDS             20408..21478
                     /gene="leuB"
                     /locus_tag="ACFM8U_000447"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC6333736.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIEQGLDVSYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLNRPLRPERGLLAIRKDLNLFA
                     NLRPAILYKELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGENKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTND
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21640..22203
                     /locus_tag="ACFM8U_000448"
     CDS             21640..22203
                     /locus_tag="ACFM8U_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC6333737.1"
                     /translation="MKKIIIAALAAAAIGTASAATYKVDEYHANARFSIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTSIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5418 a         5878 c         5794 g         5279 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagc
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatactc caacttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggcg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 aattttccgg atgatggtta ttaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccacacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcaaattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggtcg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gagtgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacctgaacg gatgaccggc aacgatgtga tgcagatgct
     3301 ggattcgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatac cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcacccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 agcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg caatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggtgt gaatgtattt atgctgtcgc ccgatggcaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggatattgtg aaggcggtgc agaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaacgcc acttcaagca atgacgaaac
     4201 cgtcagcttc tgcgtaccga gcggcaactt cggcaacgtt tgcgcgggac atattgccaa
     4261 acagatgggc ctgcctgtcc gccgcctgat tgttgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cataccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccgtct atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtgggcggaa gtagcggcgg gcaaaggctt
     4501 tgacctgcgg tttgctttag aaaaagtcgg tggcaaatac ggttttacct ccggcaaatc
     4561 cacccacccc gaccgcctcg ccaccatcag acaggtttat gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggttgtgtgt ttggaaaccg cgttggcagc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatattcc gtacaggggc attccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ctatactgct ggacgaactg gcggcaaaac aggaggcagc
     4981 catcgctccc cacctgcttg ccgacggaac caaggtatgg gtacgcaaag caggcaggca
     5041 caatgcgcgg tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gttcccagcc tcggcggcga gcctgccatt acaaccgaat caaaacgcct
     5161 gtacgaattg cgctcggcgg ggatagccgt tcccgaattg ctcgccgtca ggaaaaatgc
     5221 ccttatgttc ggcaatttgg caggcatccc gctcgatacg caaatccgcc aagaagccga
     5281 agccggaaag gcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acagcagttc ctcagtcagg cgtttgcgcg gaatatgatg tcggacggaa aaaacatcag
     5401 ctttctagat tttgaagacg acccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacatcc attcgaccgc gctgatattg aaaaacggcg gactgctgga
     5521 agcggctgcg gaaaaatggg gcggcgtact gtctgagcag cctgccgaaa tacaaaggct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cccgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atataccgcc
     5701 ataagccgcc gcctgccccg cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttggcttta ccccgaatcg
     5821 ggcagacatt ttcactctgc cgcacatccc gaacaagcac tatccatttc ttttactgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggacagt
     6001 tcgttatggt cggattgatg gtggacggca agccgctgat gcgtgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaactctagt tgccggcgac ctgaatccag gcaaacacct ttacttgttg agcaccggta
     6241 ccggcatcgc ccctttcttg agcatcacca aagaccccga gatttacgag caatttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gcgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcccgagaa gaattcgaac accacggccg cctaaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaatcaa tcctcaagac gaccgcgcga
     6541 tgctgtgcgg cagccccgcg atgctgaaag atacctgcaa agttttggac gatttcggac
     6601 tgaccgtctc tccgaaaaca ggcgtacgcg gcgattacct gattgagcgt gcgtttgtag
     6661 atcaataagc tgtttgaaaa tcccgtctga acggcaccag caatcaaaaa ccccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg aattaaattt aaatcaggac aaggcggcga
     6781 gccgcagaca gtacaaatag tacggcaagg cgagccaaca ctgtaccggt ttaaatttaa
     6841 ttcactatat agtgggtaaa aattagaaat acacatttcc gtcattcacg cgcaggcggg
     6901 aatccagacc tatccgttaa ttagcatttg aaaataaaag aaaaaccaag catatggatt
     6961 cccccctacg cgagactgac gacggtgttc tgcagtagcc aaagcagaaa accatcctta
     7021 ttgtcatttc atcatgatat aacaataagg atgttaaaaa ttagttatca ttatatgcat
     7081 gatatataag aaaaattgtc ccaataagaa atgttatgca gaaaataaaa tgaaaaggat
     7141 ggttttcatt gaaaatggtt gttctgccac cgtcatacac agaaccggta tttggaaaaa
     7201 aaataatcca tattgacatc attattccat ttatcgcata aatccacttt ttaatttttt
     7261 tcctcattaa tacaatctct tatttaataa aatcaaatac ctagcaattc gatctcaaat
     7321 tttgaacgcc aacgtgtttg agcggtaaca atacatactt taagtttgca attcttcatc
     7381 gtcttatttt tgttttcatc aactatacgt ctaaagcaac gccgtccgtc attgttcgga
     7441 cggcattttt tcagactgca ttttcccccg tttgtgaagg cgcagggcgt tgcccaaaac
     7501 cgccagcgaa ctgaagctca tacccagtgc ggctatccac ggttggacat agcccaaaac
     7561 ggcaagcggt acggcaatga tattgtacgc gcccgcccat atcaggtttt gccggataat
     7621 atggcgggtg cgccgcgcct gatcgagcag gtgggcgacg gtacgcaaat cttcgttcaa
     7681 taacacaatg tccgcgccgt ccctcgcaat atccgtcccg cccgctgcgg cggcggatac
     7741 gtctgcctgc gccaaaacgg gcgcgtcgtt gatgccgtcg cctatcatca gcactttttt
     7801 cccttctttt tgcaaggctt tgacgtattc cagtttgtcc tcgggcatgg cttgggcgcg
     7861 gtagtgcgcg acacccaggg cgcgtgcggt ttcggcaacg gcggtttcgc ggtcgccgct
     7921 gaggatgtgc agggtcaggt ttttgcctgc caactgccgc accgcctccg ccgcgctgtc
     7981 tttcaagggg tcggtcaggt agaacacggc ttggaaaccg ctttgactgc cgaggtaaac
     8041 cgcgctgccg ccgccttctg tttgcggttc tttacctgaa atttcggcga cataggatgc
     8101 cctgcccaat gcccaaacct gtgtttcgcc gttgacggtc agttgcgcgc ccacgccttc
     8161 gccgatgcgg ttgaggcgtt gtttaatagc aatgtcgggg acgctgccgt ctgaaacacg
     8221 gtgtttgagg atggcgcggg caaggggatg ttcggactgt tgttctaaag cctgcgccac
     8281 cgcgagaaca aaggcttcgt ctgtgcctct caacaatgag atacggcgga cggcgggatt
     8341 gccttgggtc agcgtgcctg ttttgtcgaa aatgatgtcg gtggtttggg cgagggtttc
     8401 gattgcctgc tttccgccga ttaaaatacc ttcgcgcgcc agcgtaccgg tagaagctgc
     8461 cagcgcggtc ggcgtggcaa gcgataaggc gcaggggcag gtaatgacca gcagggcgac
     8521 ggtaatccac aatgcggtgt gcgcgtcggc gtacagcgtc cagccgatga agacggggac
     8581 ggcaagcagg agttcgccga atatgaaaga cgaggcgtat tgttccgcca actcggcagt
     8641 gcgcggtttt tgcgctaagg cgcggtcgag caggcggacg atgtgcgaca gtcgcgtgcc
     8701 gccgccggtg cggtcggtgc gtataatcag ggggctttgc gtgttgagtg tacctgcggt
     8761 tactttttca gacggcattt tggcgacggg caggctctcg ccggtcagca tagattcgtc
     8821 aaccgtgctt gcgccttcca gcaccgtgcc gtcaatgggg atggtttcgc ccggtttgac
     8881 cagcacgata tcgcccgcct tcaacttgac gacagctgcc tcgcaggttt cctgcgtatc
     8941 ggggtaatcg ggcatatgat ggcaaaacgc aggaatcagc ttcaccagcc tctcggcggc
     9001 atcgcctgcc ttacggcggg caatgtgttc cataaagcgt ccgcccagca ggaaaaacag
     9061 cagcatggct atggattcga aatacatccc ctgccccgca ttggtcgcaa ggctgtaaac
     9121 gccggcgata aaggtcatga tgatggcgac ggcaatcggc gtatccatgc cggcgcggcg
     9181 gtttttcagg tcgcgcaacg cgccttgata aaacgggacg gcgcaataga atacgacggg
     9241 cagcaccatt aaaaagccgc cccaatggag gatttgcagg aaatcgggtt cgatgtcgcc
     9301 gccgtaaagg taggtcggca gcgcgaacat catcgtctgc atcatcccca gcccggcaac
     9361 ggcgaggcgg acgatgtatt gtttgcgttc tttttggttg gcggcttcga ttttttgcgc
     9421 gtcatagggt gcggcggtgt agcctatctg cctgattttc aacagaatgt cggaaaggcg
     9481 gattttgccg tcgtcccaga cgacgcggca gcggtgcgtg ctgtaattga ggtcgatgcg
     9541 gacgatgccg tctgtacgca aaagctgctg ttcgatcagc cagacgcagg cggcgcaggt
     9601 gatgccgccg agcattaaaa ccgcctcgcg cgtgccgccg tgggtttcca caaagtcgga
     9661 ctggacttcg ggcaggtcgt acaggcggat ttggtcgagg atttcttggg gcggcagctc
     9721 ggttttttgc gcgtcggcgg tgcgttgttt gtaataactg cccaagcccg cgtcaataat
     9781 gctttgtgcg accgcctgac agccggcgca gcaggtttcg cggtcttcgt tttcgtaacg
     9841 gacagtcagg tggaggtgtt cgggaacatc cagaccgcag tggaaacagg tttttttcat
     9901 ggcatttcgg tttcgtctgt gtttggtgcg gcggcacaag actcggcaat ggcttcgcgc
     9961 agtgcgtcta taccggtatt ttcagcaacg gaaatgcgga cggcggcaat ttttcccgca
    10021 gcgtcgcgcc atatgcccgt gttttgttct tcagacggca gcaggtcggt tttgttgtac
    10081 accttgatgc acggaatatc gccggcatgg atttcttgca gtacgttttc cacgtcttca
    10141 atctgctgtc cgctgttcgg agcggcggca tcgacgacgt gcagcagcac atcggcttgc
    10201 gcggtttctt ccagcgtggc ggaaaaggcg gaaatcagtt tgtgcggcag atcgctgacg
    10261 aatccgacgg tatcggtcag gataatgctg cattcgggac tgatgtacag ccgccgcgcc
    10321 gtcgtgtcga gtgtggcgaa aagctggtct ttcgcatata tgcccgactt ggtcagccgg
    10381 ttgaacaggc tggatttgcc gacattggta tagccgacca gcgcaaacgt tttgattgtg
    10441 cccgattcgc gggacttgcg gcgcagggcg cgctgttttt tgaggttggc aagctgtttt
    10501 ttcaaggcgt tgatccgatg ggcgatcaat cggcggtcgg tttccagttt ggtttcgccc
    10561 ggccctttca tgccgatacc gccgcgctgg ctctgcagat ggccgtaacc gcgtatcaag
    10621 cgtcccgcca aatggctcaa ttgtgccaac tcaacttgca gcctgccttc ctgcgtgcgg
    10681 gcgcggcggg cgaaaatcgc caaaatcaac cctaccctgt ccagcacgcg gcatttcagt
    10741 tctttttcaa ggttgcgttc ctgcgtgggc gtaagttcat ggttgaatac gaccaaatcg
    10801 atgccgtctg cggcaactgc ttccgacagc tccgccgcct tgcccgtgcc gacaaacagc
    10861 gcggtgtgcg gacggtcgcg tttggcggtt tccacaagga cgggattgcc gcccgccgcc
    10921 ttgaccagct cgacggcttc cgccaatgcc gtctgaaagc cgttcagacg ggcggtactg
    10981 ctgcccgtac cgtctttgtc caacattacg cccaccagca tgacgcgttc gggttgcgct
    11041 tgggtactgc cgttgcggcc tgttctgcct gtcatgtatt caatcctgta aataaagggt
    11101 tacgcctcgt tccgtttctc cttccggtgc aagcgtgatg cggctgcccg ccattccgtc
    11161 aacgctgaag acgatgccgc ccgcctcgtg ccttcctatg gacaggcggc tgccgggtcg
    11221 ctgccggtag gtataacggg tacacgcttc caaacaccag cgtccgcgcc agtggatgaa
    11281 atatttttcg ccttttctaa ccgcaagtgt ctcgccgccg tttacctcaa aataagggta
    11341 atcgggtttt cgggttctaa actgccattt cgcctgcgcg cgccgtccgt tgcggacata
    11401 gtcgaatacc gccgtataaa gcgtaccgta ttccaaaggc ttgagcggga aaagcgcgaa
    11461 ttggtacgcg gtcagcctgc cgttggggtc gttgccggcg gttaaaaccc tgacggggcg
    11521 gatttcgttt ttaccctgat acagcttgaa acttttcatc gtaattttgc ctgccgcctc
    11581 ggaaaaatca atgctggcag gattgcccgt gatttcatat tccggcacgg ggtctggacg
    11641 ctcgccgtgg aaataaggca gtgcgccgtt gccgacggga taggctgtat agagcagctc
    11701 ctgtgcgggc atggcttcgt cggtgtacac gaccgcaccg ttatggcagg cgttgcggta
    11761 atatttccgt cctgcttccg gctgatttct gccttgggcg caatgccgct caaacctgcc
    11821 gttgccctga ttgaatacga gaacggtttt accgttttcg cgcacaaatg ccgctcctgc
    11881 ctcatccgta tggcggtcaa gtagggaaag gcggtggtag attgcgctca ttaatccgtc
    11941 cacttggcgt tgctgcgtgc ggatgtcgct gtcggacgat tcggcggctt cctcttccgt
    12001 gctgatgttt tcatgcacgc cgttgtagag atacccggca aggcgtgtgc gttcggtcag
    12061 cttttgtgcg gtgtagtgcg gattgtcggg atggtgttcg ccgtgtccgt cttcgggatt
    12121 gagcgtgaga tagcgtgcgt gcctgcgggc ggaattttcc aaaaccggcg cgtgtgccag
    12181 cttgtgcaaa ccgatttggg cgcggatgcg gttgaggtag tgcagcgcat cgccgccgtc
    12241 cctgatttgc ggtgcggacg gatagacaag ttcgcccgcg ggcagggatt ggttttgggt
    12301 atgatagaaa acgccggccg ccgaacccaa caggagcagc cgaataaaaa gggatttcat
    12361 aatgccgtcc tcccgacgcg gcaacggggt gttttatcaa aacggcaaac ttgccaatgc
    12421 ggtttccgat tgcagattgc caaaccggca aacctttccc cgtgccgatg ccgaacccga
    12481 tgccgtctga actttcagac ggcatcgggt gtttatttgt ccacccggat gggtgcaggt
    12541 tcgtattgtg tcgattcgtc gccgtaatac agcacgccga gtttgatggg gatgcgtccc
    12601 tgcgatttgc ggtgggcatt ggaatccctc agggaatagg cgcaaccgca atattcctgc
    12661 tggtagaagt tttcgcgttt gctgatttca atcatgcgcg cgctgccgcc gcctttgcgc
    12721 cagttgaaat cccaatacac cacatcatcg taaggcgcgg cggcgcggcg gccacagtcg
    12781 ttaatctggt tcatgttttt ccaacgggaa atgcccagcg agctggtgaa gacggggaac
    12841 ccatgttcat gcgcgtattg cgcggctttt tcaaaacgca tatcgaaaca catcgtacag
    12901 cggatgccgc gttccggctc aaactccata cctttggctt tggcaaacca ttctttgcgg
    12961 tcgttttcgt aatcgtcgtc tttgtcgatg aaggggatgc cgaacttttc cgcaaagcgc
    13021 acgttttcct cttttctgag catatactct ttgtgcggat ggatattggg attgtaaaaa
    13081 taaatggtgt agtcgatgcc ggaggcaagc atcgcctcca tcacttcgcc gctgcacggg
    13141 gcgcagcagg aatgcagcaa gactttttta tgtccgccgg gcgggacgag tatggggcgg
    13201 tcaatgtcgg taacggtagg tttgttttgt gtttccatta acttaacaat atctgtcgaa
    13261 tacggttgaa gcggctattt tatcagagcc gccgcatgta aaacagggtg tcagggcatc
    13321 agcccgagct gcctgaacga acggacggtt tgcgccgtta cgataaaatg gtcgagcagc
    13381 gacacatcga ccagcgacat tgcctgtgcc agccgccttg tgaacatgat gtcttcctgc
    13441 gaaggttcgg gcgagccgcc cggatggttg tgcgcgataa tcaggctgtc ggcatactcg
    13501 tccaaagcca gtttgacgat ttcgcggatg taaatcgtgt tttccgcaac cgtaccgcgc
    13561 gacagctctt tgaccgcaat cagttggttt tggcggttca gcagcagcgc gacgctgact
    13621 tcgacttttt cctgccccaa atgaaagcgt aaataatcgg ccaccgtatc cggatcggac
    13681 aggacgatgc tctcctgcaa ttcttccgcc aatatccgcc gcccgatttc cctgaccacg
    13741 gcaaactgtg tgaaacttgc cgtccccgtc cctttgtatg ccgacagttt gccgacctcc
    13801 gcgctcatca gcctccccaa actgccgaac tcctgcagca aataacgcgc caaatcgacc
    13861 gcactcattc cgcgcgtgcc gacgcgcagc aggattgcca aaagttcggc atcactcaac
    13921 gccgccgccc cgcgttccaa cagcttttcc ctgggtcttt cgccttccgg ccattgcttg
    13981 atgctcatgt tttccctttt ttaaatagtc tgagatgccg tctgaaacag atgttcccac
    14041 acgccaaata tccgaaatgc cgtctgaacc ggatttccgc ttcagacggc atctgtattt
    14101 caaaacagtc ttacgccgtt tcttccaact cgattgaagc cgccagaagc gacaggcgtg
    14161 ccattacgcc gtaaacgtag aggcggttgt gttcgttgtc cacgtcgcag tctttttcag
    14221 agcgcggcat acccagcgag tcccattgtt cgaatacgcg cttgcaggct tcgggcgctt
    14281 cttgggaatt atcgccgtta ccggtaggaa tgctgttaga cagcggaaca aacaccatac
    14341 cgccggcgtt gaggttttcg tccgcaccgc gcccttcgtg tacgcggaaa aagccgccga
    14401 tgacgaagcg gtccatcata tacacgacgg gttcgcacac cgcgccgttt aaggtttcat
    14461 aagtataaat cccttcttgg acaatcactt cgctgacttc caaaccttct ttgacttttg
    14521 ccattttgtt gcggttttta cggttcaagc cgcgcacttc atccgcagat ttgacgctca
    14581 tcacgcccat gccgtaagtg ccggcatcgg ctttgacgat gacgaaaggt ttgtcggtaa
    14641 tgcccgattc gtcgtatttg gcttgaattt tcgccagcac acgttctacc gcttccgcca
    14701 acgcgtcttc gccttcacgc ccttggaagt ccaaaccgcc gattttttca aaataagggt
    14761 taatttgcca ttcgtcgatg ccgattaact tggcaaattc ggcggtaact tggttgtacg
    14821 cgccgaaatg atttgttttg cggcgcgtcg tccaaccgcc gtgcaacggc ggcaaaacgg
    14881 tttggctgat gcctttgagg atgtcgggaa tgccggcaga caaatcgttg ttcaacaaaa
    14941 ccacgcaagg cgaaaagccg tctgcaagat ggacgcggtc gcgggtacgc aataaaggtt
    15001 ccaacaggat tttgtcgccc aatgcagttt caaattcggt cggttcggtt acttccggat
    15061 tcaggctgcc caagcgcact tcataccctg ccgaacgcaa aatctcgccg agggcgtaaa
    15121 tgttttgcag gtaaaacgta ttgcgcgtgt ggttttcggg aataatcaat acggatttcg
    15181 ccgtttcgca ggcgcgttgc accgcatctt gcgcggcaac cgccgccagc gggataaagt
    15241 tgggattcag attattgaag ccgccgggga acaaattcat atcgatagac gaaattttgt
    15301 aaccggcatt gcgtatatcg accgaaccgt aaaacggcgg gcggtgcgcg ttccactgcg
    15361 tgcggaacca cgcctcgatt ttggcgtgat tggacaggat ttttgcctca aacgcctgaa
    15421 gttgcgccga atgttcgggc gacatcaccg gtaatttcat ctttcgtctg cctctgtgtg
    15481 ttttttgaca agatgcagat gataatgcct ttgaaagaat gacgacaagt attctttcgt
    15541 atcgaatcat gctttagata taactgttta ctgaaatgga caatatgtct aattttttcg
    15601 ttaaaattgt ttgcaaaata acaccggctc aaaaattaga caaaatttgt tgcgcggtat
    15661 aaagaatacg tctaaaatcc gcccaatccg cattaccatt tatccaaaag aacaacatca
    15721 tgacagcaca aaccctctac gacaaacttt ggaacagcca cgtcgtccgc gaagaagaag
    15781 acggcaccgt cctgctctac atcgaccgcc atttggtgca cgaagttacc agccctcagg
    15841 catttgaagg cttgaaaatg gcggggcgca agctgtggcg catcgacagc gtcgtctcca
    15901 ccgccgacca caacaccccg accggcgatt gggacaaagg catccaagac ccgatttcca
    15961 agctgcaagt cgatactttg gacaaaaaca ttaaagagtt tggcgcactc gcctattttc
    16021 cgtttatgga caaaggtcag ggcatcgtac acgttatggg ccccgaacaa ggcgcgaccc
    16081 tgcccggtat gaccgtcgtc tgcggcgact cgcacacttc cacccacggc gcattcggcg
    16141 cactggcgca cggcatcggc acttccgaag tcgagcacac catggcgacc caatgtatta
    16201 ccgcgaaaaa atccaaatcc atgctgattg ccgttgacgg caaattaaaa gcgggcgtta
    16261 ccgccaaaga cgtggcactt tacatcatcg ggcaaatcgg cacggcgggc ggcacgggtt
    16321 acgccgtcga gttcggcggc gaagccattc gcggcctttc gatggaaggc aggatgaccc
    16381 tgtgcaatat ggcgatcgaa gccggtgcgc gctcaggcat ggttgccgtc gaccaaacca
    16441 ccatcgacta cgtaaaagat aaacccttcg cacccgaagg cgaagcgtgg gacaaagccg
    16501 tcgagtactg gcgtacgctg gtgtctgacg aaggtgcggt attcgacaaa gaataccgtt
    16561 tcaacgccga agacatcgaa ccgcaagtca cttggggtac ctcgcctgaa atggttttag
    16621 acatcagcag caaagtgccg aatcctgccg aagaaaccga tccggtcaaa cgcagcggta
    16681 tggaacgcgc ccttgaatac atgggcttgg aagccggtac gccattaaac gaaatccccg
    16741 tcgacatcgt attcatcggc tcttgcacca acagccgcat cgaagacttg cgcgaagccg
    16801 ccgccatcgc caaagaccgc aaaaaagccg ccaacgtaca gcgcgtgttg atcgtccccg
    16861 gctccggttt ggttaaagaa caagccgaaa aagaaggctt ggacaaaatt ttcatcgaag
    16921 ccggttttga atggcgcgaa ccgggctgct ctatgtgcct cgccatgaat gccgaccgcc
    16981 tgaccccggg tcaacgttgt gcctctactt ccaaccgtaa ctttgaaggc cgtcaaggca
    17041 acggcggacg tacccacctc gtcagccctg ccatggcagc cgcagccgca gtgaccggtc
    17101 gctttaccga catccgcatg atggcgtaag tattttcagg cggtctttaa gaatttgacg
    17161 cggccgtctg aaatatgtcc cgtaaggcag aaacaccttt aatattcaaa ccaaggagtt
    17221 taaccatgaa caaacttttc attaccgccc tgtccgccct cgccttgtcc gcctgcgccg
    17281 gcacttggga gggcgcgaaa caagacaccg cccgcaacct tgacaaaaca caggccgccg
    17341 ccgaacgcgc cgccgaacaa acaggcaacg ccgtcgaaaa aggctgggac aaaaccaaag
    17401 aagccgtcaa aaaaggcggc aatgccgtcg gacgcggcat ttcccatctc ggcggaaaaa
    17461 tcgaaaacgc caccgaataa ccgattcgcg atgccgtctg aaaccgcttc agacggcatc
    17521 gtcagcacaa gacgcaaacc catgaaagcc tttaccaaaa ttaccgccat cgtcgccccg
    17581 ctcgaccgca gcaacgtcga taccgacgcc atcatcccca aacaattttt aaaatccatc
    17641 aaacgcagcg gcttcggccc caatgccttt gacgaatggc gttacctcga ccacggcgaa
    17701 ccaggcatgg acaacagcaa acgcccgttg aaccctgatt tctcgctgaa ccagccgcgt
    17761 taccaaggcg cgcaaatcct gttgacgcgt aaaaacttcg gttgcggctc ttcacgcgaa
    17821 cacgcccctt gggcattgga cgactacggc ttccgcgccg tcatcgcccc cagcttcgcc
    17881 gacatcttct ttaacaactg ctacaaaaac ggccttttac ctatcgtgtt gaccgaagaa
    17941 caagtcgatc ggcttttcaa agaagttgaa gccaacgaag gctatcagct ctccatcgac
    18001 cttgccgaac aaaccctgac cacaccaagc ggcgaaacat tcacattcga cattaccgaa
    18061 caccgcaaac actgcctctt aaacggcttg gatgaaatcg gtctgaccct gcaacacgcc
    18121 gacgaaattc acgccttcga agaaaaacgc cgccaaagcc agccttggct gtttaacggt
    18181 taagcctatg ccgtctgaag gccgttcaga cggcattcct gataacatat aattgttgcg
    18241 gctaatgtaa acagaggggc gataaattgc tttcatcttt taagcgggct tagcgacact
    18301 ctaaattaat gcaataatgc aactctccat ttcaaataaa tttttagatt tttaatatgc
    18361 aacaaattaa atttattgac ttattttccg aaatgagtgg catcaggaaa ggattcgaac
    18421 aagcctgtcg gaaacagtcg gttgcttgcg aatgtgtttt tacttcagaa atcaaaccgg
    18481 cagctttgga agtattgaag caaaactacc ctgatgaagt gccgtatgga gatataacga
    18541 aaattgaaac aggggatatt cccgattttg acatcctgtt ggcaggcttc ccttgtcagg
    18601 ctttttcttt cgcctgaaaa agattaggct ttgaagatac acggggaacg cttttctttg
    18661 atgtggcaag gattttaaag gcaaaaaaac caaaaggttt tattttggaa aatgtggaag
    18721 gattggtgac acacgataga aaagattcga cacaaaaaat agggcgcacc ctgaccgtta
    18781 ttttggaaac cttggaagca ttgggctact atgtttcttg gaaagtttta aatgcaaaag
    18841 atttcggcat tccccaaaac aggaagcgca tttatctgac aggcagtttg aaatccaaac
    18901 ccgacttatc tttcgaaaca agcccaagtc cgaaattaaa aaatattttg gaatcgggac
    18961 tgcctaccga aagcagccct ttcatcaaaa aattgctaaa aaaatttccc ccgtccgaac
    19021 tgtacggaaa atcagtaaaa gacaaacggg gagggaaaaa caatattcac agttgggata
    19081 ttgaattaaa aggcgcagta accgaagaag agaagcaatt gttaaatatc cttctaaaag
    19141 aacggaggaa aaaaaatggg cttcagaaat cggcatagat tggatggatg ggatgccttt
    19201 gacaaaagcg caaatttcaa ctttctataa acaccccgat ctccaaaata ttttggacag
    19261 cctgacagat aaaggctatt tggttttaga gcatccgaaa caaaaaattg gcgggcaaag
    19321 aatcaaagat gaatccctgc ccaaaggcta caatattgtt tcaggcaaaa aatcttttga
    19381 aatcaataaa atattagatc aaaacgatgt tgcgccaacc ttggttgcaa tgaatatgga
    19441 gcaccttttc gtcgttgaca acggcggttt gcgtacactt accggaaaag aagggttgcg
    19501 cttattcggc tatccggacg attattcgtt tgatattccc aaaaaagaca gatgcgattt
    19561 attgggtaat accgttgccg tccctgtgat taaggcggta tctgaaagac ttctgcatac
    19621 tttataaacc gacagggata aaatgataaa acttactgca caacaaatat ttgataagtt
    19681 gctggatgaa gaaaaaatct tatcggccaa tggtcaaatt agatttttct taggcgatgt
    19741 ggatattatc gtcaaacaaa aagatgttgt cggcaacatc attcaggaat ggcttggcgg
    19801 atgattgaga aaaagggaaa ttgaatttga tgtttcaacc aatacccaaa tgcctcccga
    19861 tttcttttta aataaaaaag accgcagcag agaattgttg gaagtaaagg cgttcaaccg
    19921 aaatgccggc ccgggttttg atattgcaga ttttaaaatg tattctgata aaatcattca
    19981 taagccctat atgctggatg tagactattt aatattcggt tacgatatgg acgacaacgg
    20041 caatgtaacc atcaaggatt tatggcttaa aaaagtatgg caaattacga gaagcatgga
    20101 tgggtgggca attaatcttc aagtcaaaaa aggcgtggtg cataaaatcc gcccgggtgt
    20161 ttggtacagc ataaataaaa agaatatgcc tatgtttgaa tgcttggaag attttgtttc
    20221 cgcaattgaa gaaaccgttt atcaaaaccc ggcaacacgg cataacgcat ccttatggaa
    20281 aaagaaattt gaggaagcct ataaaaaaca ctacaaccga tcgatttcca tacctcgttg
    20341 gcacgaaatt gcacacaaat acaaaaagaa ataacccttc aaccatcaac ccataaggaa
    20401 acttgaaatg accaaacata tcgccatcct ccgaggtgac ggcatcggtc ccgaaatcgt
    20461 cgccgaaacc gtccgcgtac tcgacaaact tatcgaacaa ggtttggatg tcagctacga
    20521 atacgcacct ttgggcggcg aagcctatga cgaatacggc catccttatc cagaattcac
    20581 gcaaaacctt tgccgtaaag ccgatgcggt cctgcttggt gcagtcggtt ctcctcaata
    20641 cgacaatctc aaccgtccgc tgcgccctga acgcggctta ttggccatcc gtaaagactt
    20701 gaatcttttt gccaatttgc gccctgccat tttgtataaa gagctggcca atgcttctac
    20761 tttgaaacca gaaatcgtag ccggcctcga catccttatc gtacgcgaac tgaccggcga
    20821 tatttacttt ggcgaaccgc gcggcatccg tgttttggaa aacggcgaac gcgaaggcta
    20881 caacaccatg aaatacagtg aaagcgaaat ccgccgtatt gcccacgttg ccttccaatc
    20941 cgcccaaaaa cgcagcaaaa aagtctgctc cgtaggcaaa gccaacgttt tggaaaccac
    21001 cgaactttgg cgcgaaatct ttgaagaaat cggcaaacaa taccctgatg tcgagctttc
    21061 ccatatgtac gttgataacg cagccatgca gctcgtgcgc gcgcccaaac agtttgacgt
    21121 gattgccacc ggcaacatct tcggcgacat cctctccgac gaagcctcca tgctgaccgg
    21181 ctccatcggt atgctgcctt ccgcttcttt ggacgaaaac ggcaaaggcc tgtacgaacc
    21241 gtctcacggc tccgctcccg acatcgccgg agaaaacaaa gccaacccgc tggccaccat
    21301 cctctcgctt gccatgctgc tgcgttacag cctgaacgac gaagcgcgcg cgcaacaagt
    21361 tgaaaacgcc gtacaaaaag tgctgcaaca aggcttgcgt accaacgaca tttacgaaga
    21421 aggcacgaaa ctcgtttcct gttccgaaat gggcgacgcg gtactcgccg ccttgtaaaa
    21481 aacaaagcgc ggataacacc gcgctttttc atccgccctt ttctgcccgc cccgttcgtc
    21541 aaccgcctgc aaacccaccg tttaccggca ggaaatgcca taaccccccg cctatgttag
    21601 gataaacgcc tttaccggcc cacacaaagg attatcatca tgaaaaaaat catcatcgcc
    21661 gcgctcgcag cagccgccat cggcactgcc tccgccgcca cctacaaagt ggacgaatat
    21721 cacgccaacg cccgtttctc tatcgaccat ttcaacacca gcaccaacgt cggcggtttt
    21781 tacggtctga ccggttccgt tgagttcgac caagcaaaac gcgacggtaa aatcgacatc
    21841 accatccccg ttgccaacct gcaaagcggt tcgcaacact ttaccgacca cctgaaatca
    21901 gccgacatct tcgatgccgc ccaatatccg gacatccgct tcgtttccac caaattcaac
    21961 ttcaacggca aaaaactggt ttccgttgac ggcaacctga ccatgcacgg caaaaccgcc
    22021 cccgtcaaac tcaaagccga aaaattcaac tgctaccaaa gcccgatggc gaaaaccgaa
    22081 gtttgtggcg gcgacttcag caccagcatc gaccgcacca aatggggcgt ggactacctc
    22141 gttaacgttg gtatgaccaa aagcgtccgc atcgacatcc aaatcgaggc agccaaacaa
    22201 taaatcttga tgcgaatcag cacaaatgcc gtctgaacgt ccttcaaacg gcatttgtgc
    22261 cgtcttcatc aaaccgcccc gccaaccgta agccgatatg gcggagtaca acccgccgaa
    22321 agatatttca gcggcaggca gggcttgccg gaaagtccgg aaagtatag
//
LOCUS       DAXHSJ010000016        22126 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000016 DAXHSJ010000000
VERSION     DAXHSJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.016"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            153..1058
                     /locus_tag="ACFM8U_000449"
     CDS             153..1058
                     /locus_tag="ACFM8U_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6333738.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEIETVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
     gene            1104..1253
                     /locus_tag="ACFM8U_000450"
     CDS             1104..1253
                     /locus_tag="ACFM8U_000450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333739.1"
                     /translation="MNWQAVKTGKTKHAGNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(1472..2491)
                     /gene="lepB"
                     /locus_tag="ACFM8U_000451"
     CDS             complement(1472..2491)
                     /gene="lepB"
                     /locus_tag="ACFM8U_000451"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC6333740.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLRTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPTSDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(2634..4427)
                     /gene="lepA"
                     /locus_tag="ACFM8U_000452"
     CDS             complement(2634..4427)
                     /gene="lepA"
                     /locus_tag="ACFM8U_000452"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC6333741.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPASEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(4569..5270)
                     /locus_tag="ACFM8U_000453"
     CDS             complement(4569..5270)
                     /locus_tag="ACFM8U_000453"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC6333742.1"
                     /translation="MSLKTVAVIGAMEQEIKLLREMMENIKVVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            5438..6568
                     /locus_tag="ACFM8U_000454"
     CDS             5438..6568
                     /locus_tag="ACFM8U_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6333743.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            6602..7579
                     /locus_tag="ACFM8U_000455"
     CDS             6602..7579
                     /locus_tag="ACFM8U_000455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC6333744.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            7583..7933
                     /locus_tag="ACFM8U_000456"
     CDS             7583..7933
                     /locus_tag="ACFM8U_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC6333745.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            7938..8717
                     /locus_tag="ACFM8U_000457"
     CDS             7938..8717
                     /locus_tag="ACFM8U_000457"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6333746.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYWLFKKVPDMRVV"
     gene            8743..8958
                     /locus_tag="ACFM8U_000458"
     CDS             8743..8958
                     /locus_tag="ACFM8U_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC6333747.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            9059..9370
                     /gene="grxD"
                     /locus_tag="ACFM8U_000459"
     CDS             9059..9370
                     /gene="grxD"
                     /locus_tag="ACFM8U_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC6333748.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            9476..10102
                     /gene="upp"
                     /locus_tag="ACFM8U_000460"
     CDS             9476..10102
                     /gene="upp"
                     /locus_tag="ACFM8U_000460"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC6333749.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            10125..10445
                     /locus_tag="ACFM8U_000461"
     CDS             10125..10445
                     /locus_tag="ACFM8U_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC6333750.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            10696..11115
                     /locus_tag="ACFM8U_000462"
     CDS             10696..11115
                     /locus_tag="ACFM8U_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC6333751.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFPCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            11136..11897
                     /locus_tag="ACFM8U_000463"
     CDS             11136..11897
                     /locus_tag="ACFM8U_000463"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC6333752.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            11972..13243
                     /locus_tag="ACFM8U_000464"
     CDS             11972..13243
                     /locus_tag="ACFM8U_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC6333753.1"
                     /translation="MPSETSSPRKENETEVHIPAAPFIVKQSGSNALATCALVLAALG
                     LGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAALLKDNLNRQSAIQSELDRLD
                     SGVKANGEQILMTQKSYRELTKGRADWLVDETETILNLAAQQLVLTGNIQTAVGVLEH
                     IDSRLSRFDQAELLPLKQAISSDLAELKNRPYVDISGTALRIDRLETAVSGLPLMLDG
                     VLKPGVQARNEAVSASWWNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENLR
                     LRLLDARTALMQRNSEVYQSDLNNAETAVRQYFDAKSPATQSWLKELAELKALDVRMT
                     ADDGLKASLNAVRAYRDGTRMTAAENQEAEQAASEPANEKTASESAAASDVKTIEAPS
                     LPSELKPEQPAKKQTVPEKAGRSPSAKGERA"
     gene            13240..14457
                     /locus_tag="ACFM8U_000465"
     CDS             13240..14457
                     /locus_tag="ACFM8U_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC6333754.1"
                     /translation="MKTVVWIVILFAAAVGLALALGIYTGDVYIVLGQTMLRINLHAF
                     VLGLLIAVVVWYFLFKFVIGILNIPEKMQRFSLARKGRKAALYLNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMGNTELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYETVEANLHAAAKMNAGLTRLVRLQLRYAFERGDALEVLAKTEKLSK
                     AGALGESETKQYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETC"
     gene            14532..15596
                     /gene="hemE"
                     /locus_tag="ACFM8U_000466"
     CDS             14532..15596
                     /gene="hemE"
                     /locus_tag="ACFM8U_000466"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC6333755.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEAARILADYGQGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRHYHGG"
     gene            15782..17161
                     /gene="radA"
                     /locus_tag="ACFM8U_000467"
     CDS             15782..17161
                     /gene="radA"
                     /locus_tag="ACFM8U_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC6333756.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICREDRD"
     gene            complement(17433..17912)
                     /locus_tag="ACFM8U_000468"
     CDS             complement(17433..17912)
                     /locus_tag="ACFM8U_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC6333757.1"
                     /translation="MKHILPLIAASALCISTASAHPAGEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAAHRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(18037..18396)
                     /locus_tag="ACFM8U_000469"
     CDS             complement(18037..18396)
                     /locus_tag="ACFM8U_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6333758.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPLSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(18435..22049)
                     /gene="recB"
                     /locus_tag="ACFM8U_000470"
     CDS             complement(18435..22049)
                     /gene="recB"
                     /locus_tag="ACFM8U_000470"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC6333759.1"
                     /translation="MSAPIQAFDSLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQHNAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAENNSNDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRLADNAPSGTDFAFTEDAPPPAVYRGNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVAAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLITDKLKKYGFEEIWLPAVAEM
                     AEACQKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICVIDYKSNHLGADASAYTRQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         5015 a         5755 c         6247 g         5109 t
ORIGIN      
        1 gggttttatc tggcaggacg gttttttgcc ccaacggaaa atagcctgcc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt caggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gcgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg tcagcggttt ggtatcggga ctgatgctcg
      661 ccctgtcgat ggtgttcgtc cgcaaaaccc atgaaatcga gacggtggcg cttttccctt
      721 caatgatgat tttgagttta ggcggcgcgg tatcgttggt tgttccggca ttgctgatgg
      781 acggcggcgc gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 gattgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttatctcgg ttcgctgaaa cagcagtccg agccttgact ccgtccggca tcagattttc
     1081 tgccgtggca aacctgcgta cgattgaact ggcaggcagt caaaaccggc aagaccaaac
     1141 acgccggcaa cggcgcggag cggaaaatgc cgtctgaagc cctttcagac ggcatcgggg
     1201 cgggcagaat caaaaaagcc gcctccgctg gaatggcaat gcaactaaaa taatgtccgt
     1261 atttccatat gccgcccaca ccgtcagtcg gacgaaatgc cctttattca atgggatggg
     1321 tttccacaca aacggaaata tagtggatta aatttaatcc actatattat ccccgaggct
     1381 gccggcaggg aaacaaggcg tttgatgata aaaatgccgt ctgaaacagg gttcggatgg
     1441 catttttgat gttccggcgt aattttccgg cctaacggat tgccgtaccg gccctgccga
     1501 aatcgccgaa gttcatcaaa atgaacattg ccttgccgac aaccagcttg tcatccacaa
     1561 atccccagta gcgcgaatcg gcactgttgt cgcggttgtc gcccatagcg aaatagcgtc
     1621 cttcgggaac tttgcacacg aaaccgctgc cgtcgtcggc atattggcag tgttccaaac
     1681 cgctttgctc tatggaatat ccgttttcag acataatatc ggaggtatat ttgcccaata
     1741 cgggcaggga aacggccggc tgtccttctt ttttcagaat attgaaggat ttgccgtcca
     1801 aaccgctgcg gaacatatcc gtgttgtgga tttcggatgg gtcggtgtcg tcgggataac
     1861 ggtatgtgcc gtcaggaata tcggaagtgg gtttgccatt taccgtcaaa actttattcc
     1921 gatattcgac cacatcgccc ggaatgccga caatacgctt gatgtaggtc atctccggtt
     1981 gcaaaggata attaaaaacg accacatcgc cccgttcgat tttgcccgtg gggatgaata
     2041 cattgtttaa aacgggtacg cgcaggccgt aggaaaattt gccgaccaaa atgaaatcgc
     2101 ccttgatcag gcccgggcgc atcgagctgg acgggatttg gaacggttcg gcgataaacg
     2161 accggatgag gaacaatacc aaaacggtag ggaagaaact gccgaaataa tcgccgaagt
     2221 ggctgctttc cgagatttcg ggatgagtcc tcaggcggta tttgtatacc ccccaagccg
     2281 taccgcacaa tacaacgaaa atcaggaaaa cggcggtaaa gctcataaac agggacaaag
     2341 cggcaaacac gccgaccgcc gtcaggatat aggcgtattc gagaccggaa ctccattctc
     2401 cgttttcctg tcgtttcttg tcgcttttga aataaaggat gatgccggca agcagcgcgg
     2461 cagccgcacc cgacattaac attgtgttca ttgttgttcc ttaatgctta aaaacccgcc
     2521 tgtccgtgca accgttttaa ggcggcaaat tgcaaaattt gtttgcgggc gcgtgcccct
     2581 gaaattaggg cggtttgagg ggtgttcccg acgcgccgcc ctgtgtgcca gagttatttg
     2641 tcgctcacct gcaaaatcgc caagaacgcg ctttgcggaa tttccacatt gcccacttgt
     2701 ttcatacggc gtttacctgc cttttgtttt tcaagcagtt ttttcttacg cgtaatatcg
     2761 ccgccgtaac atttcgctaa gacgttttta cgcagtgctt tgacgttttc gcgggcgata
     2821 atctggctgc cgatggcggc ttggacggca atgtcgaaca tttggcgcgg aatcagctcg
     2881 cgcattttcg atgccagctc gcggcctcgg tgaaccgcgc tttgacggtg cacaatcagg
     2941 cttaaggcat cgactttttc gccgttgacc ataatatcca gcttaatcaa atcagacggt
     3001 tggaactctt tgaaatggta gtccaacgaa gcataaccgc gcgaagtgga tttgagtttg
     3061 tcgaagaagt ccatgacgac ttcgttcatg ggcaaatcgt aagtcagcat gacttggcgg
     3121 cccatatatt gcatattgac ctgaacgccg cgcttttggt tacacaaagt catgacgttg
     3181 ccgacgtatt cctgcggcac gaggatggtc gcggtaataa tcggctcgag aatggtttcg
     3241 atgctgccga tgtcgggcag tttggacgga ttttcgactt cgattttttc gccgcttttc
     3301 aacacgactt cataaaccac cgtcggcgcg gtggtaatca aatccatgtc gaactcgcgc
     3361 tccaagcgtt cctgcacgat ttccaagtgc aacagaccca agaagccgca acggaagccg
     3421 aagcccaatg cttgggaaac ttcaggctcg aattttagcg aagcgtcgtt aagctgtaat
     3481 ttttccaaag cgtcacgcaa ggcttcatag tcgtggcttt ctacggggta gagacccgcg
     3541 aatacttggc tttgcacctc ttggaagccg ggcagcggct cagaggcagg gttggcaacc
     3601 aaagtaaccg tatcgccgac tttcgcctgt cccaattctt tcacgccggt aatcaaaaag
     3661 cccacttctc cggctttgag ttcttgtttt tgaactgatt tcggcgtaaa aacgcccagc
     3721 tgctcaacct gagtttccgc cttggtgctc ataaagcgca ctttgtcttt cagcttgatg
     3781 gtgccgtttt tcacacgaat caacataact acgccgacgt agttgtcaaa ccatgaatca
     3841 acaataaccg cttgcagcgg cgcgttttca tcgccggtcg gcgcaggaat tttggcaacg
     3901 atttcttcca aaacgtcttc cacgccgatg ccgcttttgg cggaacactg caccgcgccg
     3961 accgcatcga tgccgatgat gtcttcaatt tcctgctcca cgcgttcggg gtcggcggct
     4021 ggcaggtcga ttttgttcaa aacaggcaca acctccacgc ccaaatcaat cgcggtgtaa
     4081 cagttcgcta cggtttgcgc ttccacgccc tgcgacgcgt caacgaccaa aagcgcgcct
     4141 tcgcaagccg acagcgaacg ggaaacttcg taagagaagt cgacgtgtcc cggcgtgtca
     4201 atcaggttga gttgatacac ctgcccgtcg cgtgctttgt agttgagcgc ggcggtttgc
     4261 gctttaatgg taatgccgcg ctctttttcg atgtccatgg aatcgagtac ctgcgtactc
     4321 atttcgcgca aatccaaacc gccgcaatat tggatgaagc ggtcggcaag cgtcgatttg
     4381 ccgtggtcga tgtgggcaat gatggagaaa tttcggatat ttttcattga gttgttttta
     4441 atgctgaacg gtaggtttta gaaatgccgt ctgaacaaac agcggtgcgt ccgaatatcg
     4501 ggcgcaacgt ggaaatagcc cgatattcta acggaaaacc gctgttttgg catgatttga
     4561 taaaggtttt ataaagattt gacgatttct gccaccattt ttgcagaact tgccgccgcc
     4621 gttttcaaaa attcgtcaaa gctgatgtct gccttttcgt ccgccgaatc ggaaaccgcg
     4681 cggatgatga cgaaaggcgt ttccaactga tgacaggttt gggcaatcgc cgccgcttcc
     4741 atttccactg ctttgacttt ggggaagtgc ttgcggattt ccgccacgcc ttcgctgctg
     4801 tggacgaagc ggtcgccgct gacaatcagg ccttgttcta ccgccgcgcc ttcaaacact
     4861 tccgccgcct gttttgcttt gccgaccaag aggccgtctg aaacaaatac ggcgggcagt
     4921 tgcggcactt gtccccaaac atagccgaat gcggttacgt cgacatcgtg gtgtgcggtt
     4981 tccgtgccga tgactacgtc gccgactttc aaacccttgc ccaaaccgcc cgcgctgccg
     5041 gtgttgatga cgcagtccgc tgcgaattca cggataatcc aagccgttgc aaccgccgcg
     5101 ttgaccttgc cgatgccgct caatgcaagc accatgcgtt ttcccgccaa ttcgccttca
     5161 taggcggaaa atctgccgaa agagacgact ttgatatttt ccatcatctc gcgcaaaagc
     5221 ttgatttcct gttccattgc gccgataacg gctactgttt tcaaagacat attgctgacc
     5281 tgctgtgaat ttcggataga atgccgaatt atacacgcta acacggcagg attgagtgga
     5341 ggtggtttgt ccgtgccgtc tgaaacggtt tcagacggca cggcgggttt ttggtagaat
     5401 gggaaggtac agattgtttg aagattaggg gacgaggatg tttaccgatg aaaatatgac
     5461 cgcaaaggaa gaactgttcg catggctgcg ccatatgaac aaaaacaaag gttccgacct
     5521 gtttgtgacg acccatttcc cgcccgcgat gaagctggac ggcaaaatca cccgcatcac
     5581 ggacgaaccg ctgacggcgg aaaaatgtat ggaaatcgcc ttttcgatta tgagtgcgaa
     5641 gcaggcggaa gaattttcat cgaccaacga gtgcaacttc gccatcagcc tgccggacac
     5701 cagccgcttc cgcgtcaatg cgatgataca gcgcggcgcg acggcgttgg tattccgtgc
     5761 gattaccagc aagattccca agtttgaaag cctcaacctg ccgccggtct tgaaggatgt
     5821 cgcgctgaaa aaacgcgggc tggttatttt tgtcggcggc accggctcgg gcaaatcgac
     5881 ttcgcttgcc tcgcttatcg attaccgcaa tgaaaattcg ttcggacaca ttatcaccat
     5941 cgaagacccg atcgagtttg tccacgaaca caaaaactgc atcatcaccc agcgagaggt
     6001 cggcgtggat acggaaaact ggatggcggc gttgaaaaac acgctgcgtc aggcgcccga
     6061 tgtcatcctt atcggcgaaa tccgcgaccg cgaaacaatg gactacgcca ttgcctttgc
     6121 cgaaacgggg catttgtgta tggcgacgct gcacgccaac agcaccaatc aggcactcga
     6181 ccgcatcatc aactttttcc ccgaggagcg gcgcgaacaa ttgctgacgg atttgtcgct
     6241 caaccttcag gcgtttattt cgcaacgcct cgttccgcga gacggcggca agggcagggt
     6301 ggcggcagtc gaggtgctgc tcaattcgcc cctgatttcg gagttgattc acaacggcaa
     6361 catccatgaa atcaaagaag tgatgaaaaa atccactacc ctgggtatgc agaccttcga
     6421 tcaacacctt taccaattgt atgaaaaagg cgatatttcc ctgcaagaag cattgaaaaa
     6481 tgccgattcc gcacacgatt tgcgtttggc ggtacagttg cgcagccgcc gcgcgcaaag
     6541 ttccagcccc gatttggaac tgctctgatg gcggtatgga tttccggacg gatggtttga
     6601 aatgatttat ccgtggcata atgagcaatg gcggcagatt gcggaacatt gggagcgtcg
     6661 tcccaatgca tggctgtttg ccggcaaaaa agatacgggg aaaactacat ttgcccgctt
     6721 tgcggcgaag gcactgttgt gcgaaacccc tgcaccgggc tgcaaaccct gtggcgaatg
     6781 tatgtcctgc catctgtttg gacagggaag ccatcccgat ttttacgaaa tcaccccctt
     6841 gtcggacgaa cccgaaaacg gacgcaaact gttgcagatc aaaatcgatg ccgtcaggga
     6901 aatcatcgat aatgtgtacc tgacttcggt acggggcggt ttgcgcgtga ttctgattca
     6961 tcctgcggaa agtatgaatg tccaagccgc caacagtttg ttgaaagtgt tggaagagcc
     7021 gccgccacaa gtggtctttt tgctggtcag ccacgcggcg gacaaggttt taccgaccat
     7081 taaaagccgc tgccggaaga tggttttgcc cgcgccttcc catgaagagg cattggcata
     7141 tctgcgtgaa aggggtgtgg cggaacctga ggaacgtctg gctttccatt ccggagcgcc
     7201 gctgtttgat gaggcggacg gtgtccgtgc gttgcggatt aaactgttgg atattttggc
     7261 agaaccaagg ttgttgaaga ttttggatta cgccgcgctt ttcgataagg aaaaacttcc
     7321 gctcgccgta tttgtcgggt ggatgcagaa atggctggtc gatttgggat tgtgcctgca
     7381 acacatgaaa cccgtctatt atcccgctta tgaagacagg ctgcttcaga cggtatccgg
     7441 tttccgtccg cgcaatgtat ttgcggcgga ggatatgctc aaacagcttg ccccttacgg
     7501 gtttcatact ttaaatgtca aaatgcagat cgagcatctg ctcatcaatt atttggaatt
     7561 gaagaaagag aacgggtgaa ttatgtcaga cggacaaaat attccggcaa aaatgatgtc
     7621 gttgcagctg aaagacatga atctgctgta cagctcctat atgccgtttt tggaacacgg
     7681 cggtctgttt gtgcagacca acgacgtatt ttccatcggg gacgatattc tgcttgccgt
     7741 agaaatcctc aacttcccca aactgttcct gccgaccaaa gtcgcctgga tcaatcctgc
     7801 gcgtacttcc tccaaaccca aaggggtggg gctggcattc acaaaacacg aaaactgcct
     7861 gaaagtcaaa gaccagatcg aagtcgaact gggcaacaca atcggcggca gcagacctac
     7921 gtttaccatg taacgccatg catatcatcg attcgcactg ccacctcaat tttgaaggtt
     7981 tgaaagaacg cctgcccgaa gttttgtcca acatggaagc aaacggcgtg gggcaggcgc
     8041 tcgccatcag cgtcagtagg gaaagcttct ccgaagtctt tgccatcgcc gaagcgcacg
     8101 aacacatcta ttgcaccata ggcgtacatc ccgacagcaa ggaagccgaa gaattttcta
     8161 ttgcggaaat ggttgccgca gccgcctcgc cgaaagtggt cggcattggc gagacgggtt
     8221 tggattatta ctggtgcaaa ggcgatttgt cctggcagca caaacgcttt gcagaccaca
     8281 tcgaagcagc caatcaaacc ggactgcccg ttatcgtcca tacgcgtgat gcggcggcgg
     8341 acaccttgtc tatcctgaaa gaatgccggg ttaattcggg cgttatccac tgtttttccg
     8401 aagacatcgg ttttgcccgt gcagcaatgg atttggggct ttatatttct ttctcgggaa
     8461 tcgttacctt taaaaacgca cccttggttc aggaggcggc aaaatatgtg ccggacgacc
     8521 gcattttggt ggaaaccgat gcgccgttcc tcgcccccgt tcccaaacgc ggcaggcaga
     8581 acgaaccggc ttttgtgcgc cataccgccg aacatatcgc caaattgcgg aaccaaacat
     8641 tggaacaggt tgcggcatat acgacggaaa acttttactg gctgtttaaa aaagtacccg
     8701 atatgcgggt cgtctgaccc tgtaccgacg ataaggaaaa ccatgaaagc aattcatccg
     8761 tatgcatgtc cgcgctgctg ccggctgcct gccaacacgt ttcggacagg catggcaaat
     8821 tccgcttcca aattctgcat tgccaaaggc ggcagacggg aagtaaaaaa agacgaaagc
     8881 ggcggcggat atgccctgtg ccatttgccg gacagtagga ttgtcgagga gtgggaatat
     8941 ttccgttcac aacattgata ctgcgcgata tacggcaaat attgtgggaa gtttccgctt
     9001 ttgcgtataa tgcgtcctac ctgacaaatt ttgtcaactt tatcaaaagg aataagcgat
     9061 ggcttccatc cacgaccaaa ttaaagaagt agtaacgaca caccgcgtcg tattgtttat
     9121 gaaaggtacg aagcagtttc cgcaatgcgg tttctcttcc cgcgccgtgc aaatcctgaa
     9181 cgcggcaggc tgcaccgatt acgttaccgt caacgtattg gaaaatcccg aagtgcgcca
     9241 aggcattaag gaatacagcg actggccgac catcccccaa ctttatgtga acggcgagtt
     9301 tgtcggcggt tcggacatcc tgatggaaat gtatgaggca ggcgagctgc aagagctgct
     9361 gaaagcctga tggattcggc aatgccgtct gaacgtgttt cagacggcat tttcttttcc
     9421 ggcaaatcaa aaaaaagtat aatggcgcgt ctcaaaatca cactggaaca ccgcgatgaa
     9481 cgttaatgtt atcaaccatc cgctcgtccg ccacaaatta accctgatga gggaggcgga
     9541 ttgcagcacc tacaaattcc ggacgcttgc catcgagctg gcgcgcctga tggcatacga
     9601 ggcaagccgt gattttgaaa tcgaaaaata ccttatcgac ggatggtgcg gtcagattga
     9661 aggcgaccgc atcaagggca aaacattgac cgtcgttccc atactgcgtg caggtttggg
     9721 tatgcttgac ggcgtactcg acctgattcc gactgccaaa atcagtgtag tcggactgca
     9781 gcgcgacgag gaaacgctga aacccgtttc ctacttcgag aaattcgtgg acagcatgga
     9841 cgaacgtcct gccctgatta tcgacccgat gctggcgacg ggcggttcga tggttgccac
     9901 catcgacctt ttgaaagcca agggctgcaa aaacatcaag gcactggtgc tggttgccgc
     9961 gcccgagggt gtgaaggcgg tcaacgacgc gcatcccgac gttacgattt acaccgccgc
    10021 gctcgacagc cacttgaacg agaacggcta catcatcccc ggcttgggcg atgcgggcga
    10081 caagattttc ggcacgcgct aactgactga ttttcggagt tgatatgaat tttcaagact
    10141 atctcgccac atttccttca atcgaccatc tgggcggttt ggacgttcag gatgcagatg
    10201 gcaaaacggt tcaccacatt cctgccgttc agggtaagct cggttcgctc aagttgtaca
    10261 acgctttggc agaacgtttt gacggaaaat tggacaaaga agcggcagaa cagggtttga
    10321 tatggtttgc cgaacacgtt gccgacgcgc gcgcccatcc cggcaagcat cccaatatcg
    10381 acctgcttga aaaagttgtg caaagcggcg aaaccttgct gctcaagccg cttgccgcgc
    10441 aataattttc gaccatgccg tctgaaatcc gtttcagacg gcattttgtc ggaaagaaga
    10501 ccgtaaaacg ggcattttct tttctatttc aggatacggg caatgatgtt tcaacacaca
    10561 ggacgacaca taaagcgttg ccctatgtgt tgccctaatt tggaaggggt tacacccctc
    10621 tcaaataaaa tctgatgctg ctgccacgaa ggacggatgt ccgagtggcg gagtttcaac
    10681 cattaaggaa atacgatgaa aaaaatgttc ctttctgccg tattgcttct gtcggctgcc
    10741 gcccaaaccg tgtgggcgga tacggtgttt ccctgtaaaa cggacaacaa caaatatata
    10801 gaagttcaaa aaatcaaccg caatctttac gaatattcgt tcggcagtgc ggcaaaaaaa
    10861 gaaattgcca tacgcaacag caaagctgac ctgttggggc gttccgacag gtggcaaggc
    10921 atgggtagcg gacgccgggc aacgatgaaa ttccaaaacg gcgaatttat gtacaccata
    10981 tggacaggct tcgattccgt gactcatacg gaaagcagcg gtgtcgttgt ggagcgtagg
    11041 ggcaaggaag tcgcacgggt cggctgtacg ccgaaaaccg cgcaggcgaa tttcaacgat
    11101 gacgattttt cctagtaatc ggggcggata aggcgatgga aacagcgaaa cccgtcatgc
    11161 tgattgtccg tccgtcgggc agggccgcag aagatgtcga aacttgcctg aatgccggtt
    11221 ggcgcgcgga agtgttgagc ccgattgaaa tcgaagcaga tgctgccgga ctggaacttt
    11281 tgtccgaaca atatgcccgt gcggatgccg tgttttgggt cagtccgacc gccgttgaaa
    11341 ccgccgtccc gtaccttaac ctttcagacg gcataaaggc gcagattgcc gtagggcagg
    11401 gcagccgccg cgcattggaa cgctgtttgg tcagaacggt catcgcgcct gatgacggca
    11461 acgacagcga ggcggttttg cgcctgccgg tttggaacag tctgcccgaa ggtgcgcgcg
    11521 tattgtttgt gcgcggacac ggcgggcggg attttttgat gaatgccttg caggagaaag
    11581 gttttcggac ggaggtggca gaagtctatt tcagacggca taaacctttg aactttcaaa
    11641 atttccaaac cgaaaatatt gccgccgcct atattacgtc gaccgagctg gtgcgcttgc
    11701 tgttcgggca gcttccgccg caattttccc gattcttcaa atccttgcta tactttaccc
    11761 atcatccgcg cattgcggag gcattgaagc gcgaaggcgt gtgttcggtc gaaaccgtcc
    11821 ctacgctgga agccgcgctt tcccattctt ccatttccgt ttcagacggc atggtctttc
    11881 ccggaacctc aaattaataa ggagcaaaac ggtgggcgaa cctgaaaaca aatcatccga
    11941 acccgtacgc gagatacagg catcaaaaga aatgccgtct gaaacctctt ccccacgcaa
    12001 agaaaacgaa acagaagtac acattcctgc cgctcctttt atcgtcaaac agtccggcag
    12061 caacgctttg gcaacctgcg ccttggtatt ggcggcattg ggtttgggcg caagtggttt
    12121 tttgtttgtc caaggacaga atgtcttgaa aaaccaagag ctggcattca accaaaaaat
    12181 cgacaaagcc gccttgggcg agtcggaaaa cgccgccctg ctcaaagaca acctcaaccg
    12241 ccaaagtgcc atacagtccg aactcgaccg tttggacagc ggtgtcaaag ccaacggcga
    12301 acagattctg atgacgcaaa aatcctatcg cgagttgacc aaaggacgcg ccgattggct
    12361 ggtggacgaa accgagacca tactcaatct ggcagcgcaa cagctggtgt tgaccggcaa
    12421 tatccaaacg gcagtcggcg tgttggagca tatcgacagc cgcctgtccc gtttcgatca
    12481 ggcggagctc ctgccgctca agcaggcaat cagcagcgac ttggcggaac tgaaaaaccg
    12541 tccttatgtc gatatttccg gcacggcact gcgcatcgac aggctggaaa ccgccgtatc
    12601 cggactgccg ttgatgctcg acggcgtgct gaaaccgggc gtacaggccc gcaacgaagc
    12661 cgtttccgct tcatggtgga acgtatggga aaaatccctc ggcacattga aagggctggt
    12721 tgaaatccgc cgtttggaaa acaacgatgc catgctgatt tctcccgaac aggcatattt
    12781 tgtacgcgaa aatctgcgcc tccgcctttt ggatgcgcgg actgcattaa tgcagcgcaa
    12841 cagcgaagtc tatcaaagcg atttgaacaa tgccgaaacc gccgtcagac agtattttga
    12901 tgccaaatcc cccgccacgc agtcgtggtt gaaggaattg gcggaattga aggcgttgga
    12961 tgtgcgcatg acggcggatg acggtttgaa ggccagttta aatgccgtcc gcgcctatcg
    13021 cgacggtacg cgcatgacgg cggcggaaaa tcaagaagca gaacaggcgg cttccgaacc
    13081 ggcaaacgaa aagacagctt ccgaatcggc tgccgcatcg gatgtgaaga ccatagaagc
    13141 accgtccctg ccttcggaac ttaaaccgga acagcctgca aaaaaacaga ccgtaccgga
    13201 aaaggcaggg cgttcgccgt ccgctaaagg agaacgcgca tgaaaacggt agtctggatc
    13261 gtcatcctgt ttgccgccgc cgtcgggctg gcattggctt tgggcattta caccggcgac
    13321 gtgtatatcg tactcggaca gaccatgctc agaatcaacc tgcacgcctt tgtactgggg
    13381 ctgctgattg ccgtcgtggt gtggtatttc ctgtttaaat tcgtcatcgg cattttgaat
    13441 attcccgaaa agatgcagcg tttcagtttg gcacgaaaag gacgcaaggc cgcgctttac
    13501 ttgaacaaag cgggtttggc gtattttgaa gggcgttttg aaaaggcgga actcgaagcc
    13561 tcgcgcgtgt tgggcaacaa agaggccgga gacaaccgga ctttggcatt gatgctgggc
    13621 gcgcacgccg caggacagat gggaaatacc gaattacgcg accggtacct tgcagaaatt
    13681 gcaaagctgc ctgaaaaaca gcagctttca cgctacctcc tgctggcgga atcggcactg
    13741 aatcgccggg actacgaaac ggtggaagcc aatcttcatg cggcagccaa aatgaatgcc
    13801 ggactgacac ggcttgtgcg cctgcaattg cgctatgcct tcgagagagg agacgcgctt
    13861 gaggtgttgg caaaaaccga aaaactttcc aaggctggtg cgttgggtga atccgaaacg
    13921 aaacagtatc aaaattgggc ataccgccgc cagctggcgg atgctgccga tgccgccgct
    13981 ttgaaaacct gcctgaagcg gattcccgac agcctcaaaa acggggaatt gagcgtatcg
    14041 gttgcggaaa agtacgaacg tttgggactg tatgccgatg cggtcaaatg ggtcaaacag
    14101 cattatccgc acaaccgccg acccgaactt ttggaagcct ttgtcgaaag cgtgcgcttt
    14161 ttgggcgaac gcgatcagca gaaagccatc gattttgccg atgcttggct gaaagaacag
    14221 cccgataacg cgcttctgct gatgtatctc ggtcggctcg cctacggccg caaactttgg
    14281 ggcaaggcaa aaggctacct tgaagcgagc attgcattaa agccgagtat ttccgcgcgt
    14341 ttggttctgg caaaggtttt tgacgaaacc ggagaaccgc agaaggcgga ggcgcagcgc
    14401 aacttggttt tggcaagcgt tgccgaggaa aaccgccctt ccgccgaaac ctgttgaaac
    14461 cttcagacgg catcgttgaa atgccgtctg aaaaccattt cacttgacaa acagactgac
    14521 agaaaacccg catgactctt ttaaaaaacg atactttcct ccgcgccctg ctcaaacagc
    14581 ccgtcgaata cacgccgatt tggatgatgc gtcaggcggg gcgttatctg cccgaataca
    14641 aagccacacg cgcgaaagcg ggcagcttcc tcgatttgtg caaaaacacc gaattggcga
    14701 ccgaagttac catccaacct ttggaacgct tcgatttgga cgcggcgatt ttgttttccg
    14761 acatcctgac cgtccccgac gcaatgggct tgggactgta ttttgccgaa ggcgaaggcc
    14821 cgaaattcaa acgcgccctg caacacgaag ccgatatcgc caagctgcac gttcccgaca
    14881 tggaaaaact gcaatacgtt ttcgacgcgg taacttccat ccgcaaagca ttggacggcc
    14941 gcgtaccgct catcggcttc tccggcagcc cgtttacgct cgcctgctac atggtcgaag
    15001 gcggcggcag caaagaattc cgcaccatca aaaccatgat gtattcgcgc cctgatttgc
    15061 tgcacaaaat cctcgacacc aacgcccaag ccgttaccgc ctacctcaac gcccaaatcg
    15121 acgcgggcgc acaggctgtg cagattttcg acacttgggg cggcgtgttg agcgacgcgg
    15181 cgtttaaaga attcagcctc aaatacatcc gccaaatcgt tgccggactg aaacgcgaaa
    15241 gcgaaggtcg ccgcgtgcct gttatcgtat ttgccaaagg cggcgggctg tggctggaaa
    15301 gcatggcaga aatcggcgca gacgcattgg gcttggactg gacgtgcaac atcggcgaag
    15361 cacgccgccg cgtcggcaag caagtcgccc tgcaaggcaa cttcgacccg tttgccctct
    15421 tcggtacgcc cgaatccatc cgcaccgaag ccgcacgtat cctggccgac tacggacaag
    15481 gcagcggtca tgtcttcaac ctcggacacg gcatcaacca gcacgccgat cccgaacacg
    15541 ccaaaatctt agtcgatacc gtacacgaat tgtcgcggca ttatcacggt gggtaagccg
    15601 gcaggaaacc gcccgatatg ccgtctgaag ccgagagatg gccggttggg gtaaaaataa
    15661 ggcaatgcgg caatatccgc cgtgtacgga tagtacatga cggcggcgtt gccgcattgg
    15721 cgcaatccca accgtcccta ttttcagacg gcatttttgt tttcagatgc agggaaaacc
    15781 gatggcaaaa acgcttaaaa ccctttacca atgcaccgaa tgcggcggca cttccccgaa
    15841 atggcagggc aaatgcccgc attgcggcga gtggaacaca cttcaggaaa gcttagccgc
    15901 gcccgagccg aaaaacgccc gtttccaatc ttgggcggcg gatacctcga ccgtccagtc
    15961 cctctccgcc gtgaccgcca ccgaagtgcc gcgcaatccg accggtatgg gcgaactcga
    16021 ccgcgtattg ggtggcggtt tggtcgatgg cgcggtcatc ctgctcggcg gcgatcccgg
    16081 catcggcaaa tccacgctgc tgttgcaaac catcgccaaa atggcgcaaa gccgcaaagt
    16141 gctgtacgtt tccggcgagg aatccgccca acaggtcgcc ttgcgcgcgc aacgtttgga
    16201 actgcccacc gaaggcgtga acctgcttgc cgaaatccgc atggaagcca ttcagacggc
    16261 cttgaaacag catcagcccg aagtcgtggt tatcgattcc atccaaacca tgtattccga
    16321 ccaaatcact tccgcccccg gctccgtgtc gcaggtgcgc gagtgtgcag cccaactgac
    16381 gcgcatggcg aaacagatgg gcatcgccat gatactggtc ggacacgtga ccaaagacgg
    16441 cgcgattgcc ggcccgcgcg tgctggaaca catggttgat accgtgctgt atttcgaggg
    16501 cgaccaacat tccaactacc gcatgatacg cgccatcaaa aaccgcttcg gcgcggcaaa
    16561 cgaactgggc gtgttcgcga tgacggaaaa cggtttgaaa ggtgtgtcca acccgtccgc
    16621 catcttcctt gccagctacc gcgacgacac gcccggttcg tgcgttttgg ttacgcagga
    16681 aggcagccgc ccgcttttgg ttgaaattca ggcattggtc gatgacgcgc acggcttcac
    16741 gcccaaacgc cttaccgtcg gactggaaca aaaccgcctt gcgatgctgc tcgccgtcct
    16801 caaccgccac ggcggcattg cctgtttcga ccaagacgtg tttctcaatg ccgtcggcgg
    16861 cgtcaaaatt ggcgaacccg ccgccgacct tgccgtcatc cttgccatgc tctccagctt
    16921 ccgcaaccgc cccatgcccg aaaaaaccgt ggttttcggc gaaatcggct taagcggcga
    16981 agtccgcccc gtcgcacgcg gacaagagcg gctcaaagag gcggaaaagc tcggctttaa
    17041 acgcgccatc gtcccaaaag ccaatatgcc gcgcaacgcc aaagagtttc cgaacctgaa
    17101 aatctacggc gtttcgagtt tgcaggaagc catcgatatt tgtcgggaag atagggattg
    17161 aaaggaaatg ccgtctgaag gttcagacgg catttggttt gtggcggatt gaaaacaaga
    17221 aggcataccg gcgacagata agatttgtgg caaagttacc tgtgatatgg caaaaacaca
    17281 cacgcccgtc atccccgcaa gggtgggaat ctggagtcgt ccgtttcggc aatgattgaa
    17341 aatcacggta acccaaccgc ttggattccc gacttcgtgg gaatgagggg cgtgtgtgtt
    17401 tgatttccat ccgccatatg tcggcgacgg gcttattcgc ctacggtttt ttgtatcagt
    17461 ttttcggcgt ttgccaaagt gtttgccact tcgtcaaaac cgatgcggct gccggcgatg
    17521 agggcgcgcg tatcggtata ggcggcgcgt actttgccgt ctgtttcggt aacgaggacg
    17581 cgcaggggca gttgcagggc gaaggcgggg tctttgacca tcagcggcgt gccggctttg
    17641 ggcgtgccga agacgatgac ttttgccggc tgcatcgtta agccgtttcg gtgggcggct
    17701 tcctgatggt cgatgacggc aaaaatgtcc atccctttgc tttttatggc ggtttcaagg
    17761 cggctgacgg tttcgtcaaa actgtatttt gaggtgaggg tatgcgtggt catagcggtt
    17821 tcgttttggg tggacggttc gccggcagga tgtgccgaag cggttgaaat gcagagtgcg
    17881 gatgcggcaa tcagggggag tatgtgtttc atcgtatttc cttatcggtt tgtttatcag
    17941 gcttcggacg gggcggcacg cgccgcgccc gatgttgcgc cgcgtgccgg aacgccgtat
    18001 gccgtctgaa agcctgtcct ttcagacggc attgcgtcat ttcatccctt ttttgagcag
    18061 gtcttcataa ccgccgtgat tggcaacatt tgtataacct gcttttttca gctcttgaag
    18121 ggcggcttcg gcacgccgtc cgctgcggca gtagaggttg accggcgtgt ctttgtcggg
    18181 cgcggcttcg tgtatgcggc ggacgatttg gtcgacgggg atgttgaccg cgttgtgcaa
    18241 atgcccttcg ctaaattcct gttcggaacg gacatcgatc caaacggccg gatgttgcgc
    18301 ggtttgggcg gcggataggg gtttttgcgg ggctgcctgc gcggcaaagg cggctgaggc
    18361 aatgagtgcg gcggtaatca ggtgtttgat attcataggg ttctcctgcg gttgtcgttt
    18421 taagggacgg gaagttattt tatctgttcc aaagcggcgg catctatgtc ccaacgccaa
    18481 acgccgccgc ctttgccatc caatccgcgc aaaaacaggt agcggacgga aacggcggcg
    18541 ggcggttgtc cgcgcagttt gaagtagcgt gcggcggcaa cggcgtaaat cagcgcctga
    18601 aggtaatagt ggtggtgcgc gacggcttcg tccatcgcct gccgcgtgta ggcggatgcg
    18661 tccgcgccga gatggtttga tttgtagtcg atgacgcaga tattgccgtc ggggtcttgg
    18721 cagaccatat cgataaagcc gtttaaaaag ccgttgacgg tgtggaagtc gagcgtttcg
    18781 gcggcggcac ggcagacttc gggcagcctg atgtcgtcgc gggcaaacca gtcgcgcagc
    18841 cgtttgaggc cgaagtcttc ggtgtggagg gtaaagccca tttcgggaca gcggcactcg
    18901 ggcgggatgt cggacaggca gtatgccccc gtcagcggcg ttttctggca ggcttccgcc
    18961 atttcggcaa cggcgggcag ccatatttct tcaaatccgt attttttcag cttgtcggtg
    19021 atgagggttt cctgtccggc ggctgcttgt ccgaatttga aatcttcaag aatttcgtgc
    19081 aggcacagcc ccgcctgcgt gcctttcgga aagtcgtgta tcgatatgcc gtctgaagcc
    19141 gtcggcgttt cagacggcat cgccgctacc gaggtttcgg cggcatccaa ggacgggcag
    19201 gcatcttctt cgccgccgtc gggcgtttgg gtatggcggc ttaaggcggt gaagctggtg
    19261 tggcggacaa atcggaatcc gcgttcggga atgctgtttg cggcaaattc ggcggtttga
    19321 ccggcgttgc cgcgatatac ggcaggcggc ggcgcatctt ctgtgaaggc gaaatccgtg
    19381 ccggacgggg cgttgtccgc caaacgccgc cagttgcgtt tgagcatcgc gatgccgtct
    19441 ttttcacacg cataggcacg gcggacggtt tcgcggctgt cttgcggcaa gccttcgatc
    19501 aggtaggcga gggggttgtc ggcggtatcg gaagaatagg cggcgtagat gttgagctgt
    19561 tcctctgcac gcgtcagcgc gacataaagc aggcgcaggc gttccgccat ttcttcgtcg
    19621 gcgtattgct tctgttcgtc ttccgacagt tgcgcctttg ccaacagttc ggttcggttg
    19681 gcgctttggt ggaggatttg ccagtcggac ggtccggtat cttgcgcgtc ccacgcaaac
    19741 gggcagtaca ccagcggata ctgcaaacct ttcgaggcgt gcatggtaac gattttgacc
    19801 aaatcttcgt cgctttccag acggatggcg cggttgtcgt tgctgttgtt ttcggccagg
    19861 ctgatttggt cgcgcaacca tttgtgcagc gcggcggggt tgcggttttg ggcgtcttcg
    19921 gcggcaagca gttcgagcag ttggaaataa ttggtcaggc tgcgcccgtt gttccggctt
    19981 aagaggcgcg tttcgatgcc gtgtgtttgg gaaaattgct gcatggcggc gaaaatgccg
    20041 tattgattcc agttgtcgag cgcggttcgg gcagattccg cccaatgcaa aatctcgctt
    20101 tcgttttggt tgaagtcgtg caattgctgc gcgtcataac cgaatatgcc gcttgtcagg
    20161 acaaagcgca gcgttccggc gcggcgcggt tcgagccaga agccgatgag tgcggacagg
    20221 gcggcggctt cgggcgaggc gaacacggat tcgcgcgaaa gcaggacgct ttgcacctgc
    20281 cgttttttca gggcggcgga aaccatcacc gcctcgttgt gcgtgcgtac cagcacggca
    20341 atatcgcccg actgcaacgg gcagcctttg aaattcagac ggcctccggc ggcttcgttg
    20401 agcgcgtggg cgatttcgtc ggcgcaatag tcggcggcac ggcggcgcaa aacgtctttg
    20461 ttggcttttt cattgtcgtt ttcgtgcagc caacgaacct gtacggcagg acgtttgggc
    20521 gacaacctgc tttcggcacg cgccgcaccg acttccgaat agccgatgtt ttccaaaacg
    20581 aacgggcgtt ctttgaggcg gaacagcgcg cctatgctgc cgataagcgc ggcgtggctg
    20641 cggtagttgg tggcgagcgt gtagcggtgc cgcgcatctt ccgccgcctg aaggtaggcg
    20701 taaatgtccg ctccgcgaaa gctgtaaatc gcctgtttgg gatcgccgac gaggaacagc
    20761 ggtcggtttt gggcgatgaa gattttttgg aagatttcgt attgcagcgg gtcggtgtct
    20821 tggaactcgt cgatcagcgc ggtttcccag ttttcggcaa cggcgcgggc gagggtttcg
    20881 gcgtgcggat tgtcggtcag cgcggtgtgg acatcgagca gcaggtcgtc gaaaccgcgt
    20941 tcgcggcgcg attttttcat ctcggcaagg ctgcggttga ggtattcgat taaatccagt
    21001 tgcagccgga tcattgttgc ttcttccgct tcttcgagtg cgttcaaatc gcgcccgaag
    21061 tctgccagtt tctgcaattc ggcaaatact gccgcatcgg gcgttttgcc ttttttcagt
    21121 ccggcttcga gtttgtcgga tgcaagtttc aagagtctgt cgtgcgtgtc tttgtccaga
    21181 aagggcagtt gtccggcggc ggatttttgt gccagttctt taaaaaggtt gccgaagctg
    21241 tttttgcggt aactgttgcc gttgaggtcg ggatggatgc gccaaaagcc ggcttccagc
    21301 tcgggcagca ggcggcagac ggtttgccat gaggtttcgg cgttgtgctg cgcctgtttc
    21361 aaatctgcct gcggacggcg gaaattcagg tacgggcggg acaggtaggc gcggatttgg
    21421 gcaaggacgg tttgcggcac agctttgcgt ttaaacgcca atgcggcaag caccggatca
    21481 ttgctgacgc gttcccgcca aaaatcttgc gccgggataa gcaggcggtc gccgtcttct
    21541 tcggtcagtt cgacatcgaa cggtgcttgg cacaagaagg cgtagtcgcg caggatacgt
    21601 tggcagaagc cgtggatggt atagatggcg gcgttgtcga attgcccgat ggcggccttg
    21661 aggcggacaa tcagacgcgt ccggccctct ttttgcaaag cctgttttaa gagtgcgggc
    21721 aggaaggtgt cgcctttgtg gtgttccgca caataggcgg cgatgccgtc tgaaagcgtg
    21781 tcgtctccaa gtttggcaat ttctttactt tctaaaacct gcaacacatc gtccaaacgt
    21841 gcgcgcaggc gtgttttcaa ctcggcggtg gcggctttgg tgaaggtaac gaccaatacg
    21901 cgctcgacgt ttttttgttc taatacgatc aggcgcgtaa acagggcggc aatgccgtag
    21961 gttttgccgg tgccggcaga ggcctcaatc aggttggtgc cggcaatggg gacggttagc
    22021 gagtcgaatg cttggatggg tgcagacata gtgcgcgctc ggaaaacggt tggacggtat
    22081 agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagt
//
LOCUS       DAXHSJ010000017        21988 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000017 DAXHSJ010000000
VERSION     DAXHSJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.017"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..78)
                     /locus_tag="ACFM8U_000471"
     CDS             complement(<1..78)
                     /locus_tag="ACFM8U_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6333760.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTART"
     gene            153..1193
                     /gene="murB"
                     /locus_tag="ACFM8U_000472"
     CDS             153..1193
                     /gene="murB"
                     /locus_tag="ACFM8U_000472"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC6333761.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            1205..1855
                     /locus_tag="ACFM8U_000473"
     CDS             1205..1855
                     /locus_tag="ACFM8U_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC6333762.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MITKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            1910..2686
                     /locus_tag="ACFM8U_000474"
     CDS             1910..2686
                     /locus_tag="ACFM8U_000474"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6333763.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSMGKIIRTVCDNSLSL"
     gene            2737..3303
                     /locus_tag="ACFM8U_000475"
     CDS             2737..3303
                     /locus_tag="ACFM8U_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC6333764.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            3323..4213
                     /locus_tag="ACFM8U_000476"
     CDS             3323..4213
                     /locus_tag="ACFM8U_000476"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC6333765.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            4238..4996
                     /locus_tag="ACFM8U_000477"
     CDS             4238..4996
                     /locus_tag="ACFM8U_000477"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6333766.1"
                     /translation="MNPKISSDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NRWEGDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(5051..5716)
                     /locus_tag="ACFM8U_000478"
     CDS             complement(5051..5716)
                     /locus_tag="ACFM8U_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6333767.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(6020..6793)
                     /gene="folE2"
                     /locus_tag="ACFM8U_000479"
     CDS             complement(6020..6793)
                     /gene="folE2"
                     /locus_tag="ACFM8U_000479"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC6333768.1"
                     /translation="MNTIADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGTQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHTEALDFAQLHRLTAEMVALLDSHSGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTADAEVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFIVESENFESIHNHSAYAYIAYP"
     gene            6985..8142
                     /locus_tag="ACFM8U_000480"
     CDS             6985..8142
                     /locus_tag="ACFM8U_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC6333769.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQVLEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDGLLKAHNVKLVWLETPSNPLLRLVDIKALAA
                     KAKAAGALVGNDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGVVVAKTKELA
                     QPLHDMMVHTGAIAGPMDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFY
                     PGLPSHEHYELAKAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKAGLLRFSVGIEDVDDIWNDISAALDTTV"
     gene            complement(8206..9207)
                     /gene="hemB"
                     /locus_tag="ACFM8U_000481"
     CDS             complement(8206..9207)
                     /gene="hemB"
                     /locus_tag="ACFM8U_000481"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC6333770.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEASNLGIPMLALFPVVTANKTECAQEAYNPE
                     GLVPTAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(9390..9674)
                     /gene="yhbY"
                     /locus_tag="ACFM8U_000482"
     CDS             complement(9390..9674)
                     /gene="yhbY"
                     /locus_tag="ACFM8U_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC6333771.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEA"
     gene            9784..10404
                     /locus_tag="ACFM8U_000483"
     CDS             9784..10404
                     /locus_tag="ACFM8U_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC6333772.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            10468..12435
                     /gene="ftsH"
                     /locus_tag="ACFM8U_000484"
     CDS             10468..12435
                     /gene="ftsH"
                     /locus_tag="ACFM8U_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC6333773.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            12657..13088
                     /locus_tag="ACFM8U_000485"
     CDS             12657..13088
                     /locus_tag="ACFM8U_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC6333774.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            13081..13359
                     /locus_tag="ACFM8U_000486"
     CDS             13081..13359
                     /locus_tag="ACFM8U_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC6333775.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            13412..13990
                     /gene="pth"
                     /locus_tag="ACFM8U_000487"
     CDS             13412..13990
                     /gene="pth"
                     /locus_tag="ACFM8U_000487"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC6333776.1"
                     /translation="MSNKIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            14051..14257
                     /locus_tag="ACFM8U_000488"
     CDS             14051..14257
                     /locus_tag="ACFM8U_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC6333777.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            14254..14616
                     /locus_tag="ACFM8U_000489"
     CDS             14254..14616
                     /locus_tag="ACFM8U_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC6333778.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            14847..16262
                     /locus_tag="ACFM8U_000490"
     CDS             14847..16262
                     /locus_tag="ACFM8U_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC6333779.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMIPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGSMKAAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSSAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            16381..16890
                     /locus_tag="ACFM8U_000491"
     CDS             16381..16890
                     /locus_tag="ACFM8U_000491"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6333780.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            17053..18435
                     /locus_tag="ACFM8U_000492"
     CDS             17053..18435
                     /locus_tag="ACFM8U_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC6333781.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIGVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGTFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNQFKAVI
                     ESNPNLSWPL"
     gene            complement(18455..19210)
                     /locus_tag="ACFM8U_000493"
     CDS             complement(18455..19210)
                     /locus_tag="ACFM8U_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6333782.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLDTMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(19220..19936)
                     /locus_tag="ACFM8U_000494"
     CDS             complement(19220..19936)
                     /locus_tag="ACFM8U_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6333783.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(19926..20753)
                     /locus_tag="ACFM8U_000495"
     CDS             complement(19926..20753)
                     /locus_tag="ACFM8U_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6333784.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSVPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            21038..21946
                     /locus_tag="ACFM8U_000496"
     CDS             21038..21946
                     /locus_tag="ACFM8U_000496"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC6333785.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT         5427 a         6464 c         5397 g         4700 t
ORIGIN      
        1 tgttcttgca gttacacctg cgacaaatag ctcaatgagt ttattttgtt tataccggct
       61 tagacgactt tttctcataa aggcaactct aacttaattt gaatttccct agttatctag
      121 gacagcccca aacatatttt aaagttcaaa ccatgcaacc catccgatac cgaaccgacc
      181 tcacccccta caacaccttc ggtcttcgcg cccaagcccg agcctttatt gcgcttgaac
      241 acgccgacgg gttgcgcgac atcgtccggc tacccgagtt caaccgagat actgttttat
      301 ggctgggcgg cggcagcaat atccttttga tggaggacta cgccggtctg gtcgtacata
      361 tggaaaacaa aggcatacgc gaaattgctc gttcagacgg catggttctg attgaagcgc
      421 aggcgggcga aatctggcac gattttgtcc tgcacgccgt cgcgctgggt ttgaacggtt
      481 tggaaaacct gagcctgatt ccgggtacgg tcggcgcgtc gcccgtgcag aacatcggcg
      541 catacggcgt agaggcgaaa gatgtgattc acagcgtgcg ctgcttcgat ttggatacgg
      601 aaacctttgt aaccctttcc aatgccgact gccgcttcgc ctaccgcgaa agcctgttca
      661 agcaggaagg taaagggcgt tatgtgattg tttcggtcgt atttgcatta aaaacgcatt
      721 ttgtgccgaa cttgggttac ggcgatttgg cggccgccgt tgccgaactg agcgcgggca
      781 gggtgccgac ggcgaaagat gtttccgatg cagtgtgtgc aatccgcaac agtaaacttc
      841 ctaatcctaa cgtgcttggc aatgtcggca gtttctttaa aaaccccgtc gtcagcgcag
      901 aaaaagccgc caccttgttg cagcggcatc ctgatatgcc gcgctatccg cagcccgacg
      961 gttcggtcaa actcgccgcc ggctggctga tcgaccaatg tcgtctgaaa ggctttcaaa
     1021 tcggcggcgc ggcggtacat gacaggcagg ctttggtctt agtgaacaaa aacaatgcct
     1081 cggcaaacga tgtccggcag ttggcgcaac acatcaaatt tacagtattt gctcggtttc
     1141 aggtagaatt gcacgccgaa cccaattggc tgcctgcttc gttcagcctg taaatccaag
     1201 gagtatgccc gtgacccgcc ccgccaaaat caatacttac acacgcatca tcgacgccag
     1261 ccttgcgctt ttcaacgagg aaggcgagcg caacatcagc accaaccata ttgccgccca
     1321 cttgggcatc agtccgggca acctctatta ccacttccgc aacaaagacg aaatcatcgt
     1381 ccaactgttc aaacgttaca gcgaagccct gctggaatac ctcaacgaag ccgtgttgcc
     1441 gtctgatgtg gaagactcca tcaattatat ggcgggcatt tacgacgtga tgtgggaata
     1501 ccgcttccta ttcagcgacg tgaacaccct gcttgcacgc agtgccgaac tattgggcga
     1561 acacaatacc ttcacccaag ccaaagtctc cccgctcttg gtcaacctgc tgacccagct
     1621 caacggtctg aacatcatcc aagccgacca aaccgccatg aacgacctcg ccgtcaatat
     1681 gtggatgatc acgaaatact ggttcgactt cgacagctcc ctgcgcggcc gcaccaagct
     1741 gaccgaagac tccaaagcac gcggcatccg ccgcacctta agcctccttc gtccttatct
     1801 tttgcccgaa caccgcgaga aatacgaccg gaaaatcggc aacggcaacc cgtaaaccca
     1861 cagcgtccga atcagccttc agacggcact tacccgacgg tatcagcgaa tgcgcgcagt
     1921 ccccccgccc catatctcca tcacaggcct aggttacctc ggcctgccgc tggcacaaaa
     1981 gttttaccaa cacggcagcc gcgtcgccgc cataaaacgc agcctgactt cggacgacat
     2041 caacctgccc atacatctcg acaccttcga cctcggcagc accgatgcct ttcagacggc
     2101 attgtggcgg caccacgccg acaaacccgt ctggttcttc ctgctaccgc cctcctcgct
     2161 gacgcattac gccgacaccg tcaaacaatg ggcagaactt gcccgggcgt gcaacgtgca
     2221 acacctgatt ttcacaagca gcaccagcgt ttacggcgat acagcgcgcg aatgcgacga
     2281 aaccgccaca cccgatccgc aaaccgagtc cgcccgccaa atcctcgccg ccgaacaaca
     2341 cctgctcgac agcggcatct ccaacatcga catcctgcgg ctgggcgggc tttattgcgc
     2401 cgaacgccat cccgtcagcc gccttgttca aaagcaaaac atcccgggcg gcaaccgccc
     2461 catcaacatc gtccaccgta atatcgccgt cgaaagcctg tttcagacgg catttaaccc
     2521 cggcggcagg cggctgaaaa acattatcga accgcgccac ccgacacgac gcgaattcta
     2581 cacggaagaa gccgccaaac tcggtttgcc ggctcctgat ttcgcacccg acgacagcat
     2641 gggcaaaatc atccgtaccg tttgcgataa cagcttaagc ctgtaaaata agtgtcaaca
     2701 gcaacaaaca ggcattctcc gccccgatca gaaaatatgt ccgccctcct ccccatcatc
     2761 aaccgcctga ttctgcaaag cccggacagc cgctcggaac ttgccgcctt tgcaggcaaa
     2821 acactgaccc tgaacattgc cgggctgaaa ctggcgggac gcatcacgga agacggtttg
     2881 ctctcggcgg gaaacggctt tgcagacacc gaaattacct tccgcaacag cgcggtacag
     2941 aaaatcctcc aaggcggcga acccggggcg ggcgacatcg ggctcgaagg cgacctcatc
     3001 ctcggcatcg cggtactgtc cctgctcggc agcctgcgtt cccgcgcatc ggacgaattg
     3061 gcacggattt tcggcacgca ggcagacatc ggcagccgtg ccgccgacat cggacacggc
     3121 atcaaacaaa tcggcaggaa catcgccgaa caaatcggcg gattttcccg cgaatccgag
     3181 tccgcaaaca tcggcaacga agcccttgcc gactgcctcg acgaaataag cagactgcgc
     3241 gacggcgtgg aacgcctcaa cgaacgcctc gaccggctcg aacgcgacat ttggatagac
     3301 taaccttcag acggcatccg acatgaacag cccttttcac aacatcggca tcgtaacccg
     3361 ccccaacacg cccgacatcc aagacaccgc acacacgctg attacctttt tgaagcagca
     3421 cggctttacc gtctatctcg acgaagtcgg cataaaggaa ggctgcatct atacccaaga
     3481 caccgtcggc tgccatatcg tcaacaagac cgaactgggg caatactgcg acctggtcgc
     3541 cgttttaggc ggagacggca cctttctctc cgtcgcccgc gaaatcgccc tgcgcgccgt
     3601 tccgattatc ggcatcaacc aagggcattt gggcttcctg acccaaattc cccgcgaata
     3661 tatgacggac aagctattgc ccgttttaga agggaaatac cttgccgaag agcgcatcct
     3721 gattgaggcc gcactcatcc gcgaaggcaa aaccgccgaa cgcgccatcg ccctcaacga
     3781 tgccgtcctc tcccgtggcg gtgccggaca gatgattgag tttgaagtct tcgtcaatcg
     3841 ggaattcgtc tatacccagc gttcggacgg gctgattgtc tccaccccca ccggatcgac
     3901 cgcctattcg cttgccgccg gcggccccat catgcaggca ggattacacg ccttcacgct
     3961 cgtccccatc tgcccacaat ccatgaccaa ccgccccatc gccattccag acacgtccga
     4021 aatcgaaatc ctcgttaccc aaggcggcga cgcgcgcgtc catttcgacg gtcaaaccca
     4081 tatcgacgtg caaaacctcg accgcatcac catccgccgc taccgaaatc ccttacgcat
     4141 tttgcacccg accgactacc aatatttcaa aaccctgcgc caaaaactgc actggggcga
     4201 gcaattagtc taagccggcc tttacccgaa agtatccatg aatcccaaaa tttcctccga
     4261 ccatacagaa gatgccgtgc gcctctccaa acgcatggcg caactggggc tttgttcacg
     4321 ccgcgaagcc gacggctata tcgaacaggg ttgggtaacg gtcaacggca aaaccgccgt
     4381 actcggtcag aaagtttcac cggcagaccg tatcgaactg aacaagaaag cccacgaaca
     4441 gcaggcggca cgcattacca tcctgctgaa caaacccgtc ggctatgtca gcgcacaagc
     4501 ggaaaaaggc tataaatccg ccgccgaact gattacccct gaaaatcgct gggaaggcga
     4561 caccagccgc atccgtttcg atccgaaaca caaaatcggc ctagctcccg ccggcaggct
     4621 cgacatcgac tcggtcggat tgctggtgtt gacgcaagac ggccgtatcg ccaagcagct
     4681 tatcggcgaa aacagcggca gtgaaaaaga atatttggtg cgcgtgcgcg gcaaattgga
     4741 cgaaaaagga cttgccttac tgaatcacgg attgagtttg gacggcgaga aactgcgtcc
     4801 cgcccaagta gaatggcaaa acgaagacca actgcgcttc gtgttgaaac agggtaaaaa
     4861 gcggcaaatc cgccgtatgt gcgaactggt cggactgcgc gtcgtcgggc tgaaacgcat
     4921 ccgcatgggc aaggtcaaac tcggcaggct gccgcccggc aaatggcgtt atctcgcccc
     4981 cggtgaatcg ttttaaataa gcattaaaaa atgccgtctg aaaatccttg cgttttcaga
     5041 cggcattgcc ttatgcccaa ataacggttt cttccaaggg cctgcgcgcc ttcgtggcga
     5101 tttcctgaac gcggtagccg aatgtcgcgg cgacggacac gccccattct gccgcatcga
     5161 acaaaccgaa ctgcccggac aatacgcgtt ccatatcggc atagttgaaa ccttccaccg
     5221 ggcaggaatc gatacctgcc atcgccgcac ccgtcatcat gttgcccaac gcgatatagg
     5281 tctggcggca acaccagtca aacaaggcgc gcgaatcgtc caaaatcttg atgtcgtcgg
     5341 cttgaaacgc ctgatacctc gccaaagatt ttgccatggc atccggttcg gtaacgccgc
     5401 gccgtttgag gctttccaac ataaacgggc tgtcgaagcg ggcatttttc tttgccaaaa
     5461 acaccaccaa atgactggcg gtatccaaag catccgccat accccaagaa aacggcttga
     5521 ttgcctgtcg gatttcgggg ttttgaacca caacaaactg ccaaggctcc gaaccgaccg
     5581 aactgggcga caaacgcccg agttctaaaa taaactgaaa atcctcggca ctgattttac
     5641 gtgccgcatc gtaatgccgg catgatttgc ggtttttaaa tgcggataga acctgctctt
     5701 tgcttaatac tgtcatcgtg catcctttta tagtgaatta aatttaaacc agtacggcgt
     5761 tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc gttgccttgt cctgatttaa
     5821 attgaatcca ctatattact ttgattgcaa attgatttca aaccgatacg gcactgccgc
     5881 atcataataa gccgcccccg ccgtcgggct ttatccggat tttgtccatc tcccgacaac
     5941 cggaaaagta aagcggcggc agattcgcaa atgccgccca aacgcctttc agacggcatt
     6001 tgggtcatcg caaacacgcc taaggatacg caatataggc ataagccgaa tggttgtgga
     6061 tagattcaaa attctcgctc tcgacaatga aactcttgat gcgtttgtcg gcaatcagcg
     6121 aagtagcgac atcgcgcacc atatcttcca cgaatttcgg attttcgtag gccttttcgg
     6181 taacgtattt ttcatcgggg cgtttgagca ggccgtatag ctggcagctc gcctgcgttt
     6241 ccacataatc gataacttcc tcgataccga cttcggcatc ggcagtcagg ctgacggtaa
     6301 cgtgcgaacg ctgattatgc gcgccatatt gggaaatttc tttggaacac gggcaaagcg
     6361 aggttacggg aatcatgacc ttcatactgt ggccgtatgc cccgtctttg atttcgcccg
     6421 tgaggcagac atcataatcc agtaaagacc gaataccgga aaccggggcg gttttcttgc
     6481 ggaaaaatgg gaaggaaaca ctgattttac cggagtggga atccaaaagc gcgaccattt
     6541 cggcagtcag cctgtgcaat tgtgcaaaat ccaaggcttc ggtatgttgc tccatcaatg
     6601 cgacaaaacg cgacatatgc gtacccttct gctcggcggg caggtaaacc gtcatggtca
     6661 gacgggcaac tgtggattgc gttccttcgg cggtttgcag ggtaatcgga aagcgcaggt
     6721 ctttaatacc gacctgattg atcggcagat tgcgcaaatc tcggctggat tgcacgtctg
     6781 caatagtatt catgagttgt ttttccttaa tattcatgta attgttgaga tgtgtgcatg
     6841 gtatggcgtt tccgccgggg aatataccgt caatctgcaa atgcgagatt atatcactcg
     6901 cttttagcag ctgcattgat tgtttctatt tttcagatag tgataaaatc tttgattatt
     6961 cacatctaca taggaagaga cccaatgaaa ttcgccacga aagccattca ttccagctac
     7021 gattgcgacg aacacaaccg cgcgctgatg ccgccgattt atcaaaacag tatgtttgcg
     7081 ttgcacgaga ttggcgaaaa tgtgccttac cgctactcgc gcctgagcaa cccgacccgt
     7141 caggtattgg aagataccgt tgccgatctg gagcacggtg cggcaggttt tgcgttttcc
     7201 agcggtatgg cggggattga tgccgtatgg cgtactttcc tgcgcccggg cgatacgatt
     7261 gttgccgtcg ccgatattta cggcggcgct tatgatttat tggtcgatgt ttatcaaaaa
     7321 tggggggtga acgttgtttt tgccgatttg ggcaatccgg ataatttgga cgggctgctc
     7381 aaagcgcaca atgtcaaact ggtttggctg gaaacgccgt ccaatccgct tttgcgcttg
     7441 gtagacatta aagccctcgc agcgaaagcc aaagcagccg gtgcgctggt cggcaacgac
     7501 aacacctttg ccacgccata tctgcaacag ccgttggata tgggttgcga ttttgtgttc
     7561 cattccgcta ccaaatattt gtgcggccat tccgacgtat tgatgggtgt agtcgttgcc
     7621 aaaaccaaag agctggctca gcctttgcac gacatgatgg tgcatactgg cgcgattgca
     7681 ggcccgatgg actgctggct ggtgttgcgc ggtatcaaaa cgctggctct gcgcatgaac
     7741 gcccattgcc aaaacgcact cgaaatcgcg cgccgtttgg aagcccatcc cgccattgaa
     7801 aaagtgttct accccggcct gccgtctcac gaacattacg aactcgccaa agcacaaatg
     7861 cccaaaggca tcggcggcgt agtgacggtt tatctcaaaa acgatacgcg tgaagcggca
     7921 aacagcgtga ttaaaaacat gaaactggtc aaaatggctt ccagcctcgg cggcgtggaa
     7981 agtttggtca atcattgcta ttcccaatca cacagcggcg tgccgcatga tgtgaaaatg
     8041 gaaatgggca tcaaagccgg cctgctgcgt ttctctgtcg gtattgaaga cgtagatgat
     8101 atttggaacg atatttccgc cgcactcgat acgactgtat aatccacgca aaatgccgtc
     8161 tgaaaccata gtttcagacg gcatttcaat taacccgacc gaaaatcaac gcttcagcat
     8221 ctttgccgcc tcaatggcgt aataagttaa gataccgtcc gcacccgcgc gtttgaatgc
     8281 cagcaggctt tccaaaacca ctttgccgcc gtccagccag ccgttggcaa ccgcagcctg
     8341 cagcatcgca tattcgcccg aaacctgata ggcataagtc ggtacgccga actcgtcctt
     8401 cacgcggcgg acaacgtcca aatacggcaa accgggcttc accatcacca tatccgcacc
     8461 ttcctgaata tcgagcgcca cttcgtgcag cgcctcgtcg gtatttgccg ggtccatctg
     8521 gtaggttttc ttgtctgcct tgcccaaatt gcccgaactg cctaccgcat cacggaaagg
     8581 gccgtagaat gcagaagcat atttggcgga atatgccata atccgcgtat ggatatgccc
     8641 ggcatcctcc aaagcctcgc gaatcgcgcc gatacgcccg tccatcatat cggaaggggc
     8701 aacgacctgc gcgcccgcct ctgcatgaca caaagcctgt ttcaccaaga cctctacggt
     8761 ttcatcattc atcacgtaac cgttttcgtc cgtcagtccg tcctgaccgt gaaccgtata
     8821 aggatcgagc gcgacatccg tcataatgcc cagttcggga aacctctcgc gcaaggctcg
     8881 gacggccgtc ggcaccagtc cttcagggtt atacgcctcc tgcgcacatt ccgtcttatt
     8941 tgccgtaacg acagggaaca gtgccagcat gggtattccc aaatttgacg cttcctccgc
     9001 cgtaaacagc aacctgtcca aactttgacg cttcacgccc ggcatagaag gcacatcctc
     9061 ctcgcgcgcc gacccctcca atacgaacac cggataaatc aaatcatcgg cggtcagcgt
     9121 gtgttcgcgc atcaggcggc gtgaaaaatc atccctgcgc atacggcgca tacgcgaagc
     9181 cggaacattg cggtaaggaa actgcataag ccctccaatc atcctgtcaa caattcaaac
     9241 catacggaag ccgccgcaac ttgcagacag catcgggcgg caatcaaaat ccgttttcac
     9301 cggaaagggt ttcggcccgc cgaaatgccg cgccgaagga cggcgtcccg cccattaaag
     9361 aacaatagca aaacaaccgc ttcaggctgt caggcttcga tattcttacg ccacaatacc
     9421 aaaagtttcc cgatatgctg aacaggttgc gcgtcaaccg cctcacataa ggcagtgcag
     9481 atttcgatac gttcggcacg gtcgtcgccg aatacgcgca ctttaatcag ctcatgcgcc
     9541 gtcaatgccg catcggtttc cttgatgacc gcgtccgtca gaccctgctg accgaccatc
     9601 acaacaggat ggagatggtg cgcgcgcgct ttcagttcca aaatttcttt ggtgttcaat
     9661 ttggtatccg tcattttcct actgcttgaa taaaaagaat aatgcggcat tgtacgcgat
     9721 ttggcacgac atcggcaaaa ttaacgtaca atacgcggtt tccattccga cccgcgaacc
     9781 actatggctg tacgttccaa atcctcaaaa gcgtggctgc acgaacacgt caacgaccac
     9841 tacgtccata tggcgcaaaa agacggctac cgcgcccgtg ccgcatacaa acttttggaa
     9901 atcaacgaca aagacaaatt aatcaaaccc ggcacggtac ttgccgactt gggcagcgca
     9961 ccgggaagct ggtcgcaggt tgccgccaag ctgacgggta cttccggagc agttttcgcc
    10021 ttggacatcc tgcctatgga agccataggg ggcgtctcct tcattcaggg cgacttccgc
    10081 gagaacgacg tactggcaca attcgaaacc ttgttggaca accgcccgct cgaccttgta
    10141 atttgcgata tggcgcccaa tatgtcggga aacgccgtaa gcgatcaggc acgcagcttt
    10201 tatctgtgcg aactggcttt agacttcgcc tcgcaacacc tgaaaaccgg cggcagcttt
    10261 ttggtcaaag tctttcaggg tgcaggctat caggaataca tggcagccat gcgcgaaatt
    10321 ttcggcacgg tgcagacgcg caaacccgaa gcctcgcgca atcgctccag tgagatttat
    10381 ttattgggca aaaataaacg ctgacaatac agacggcgtg ctttacaatc atttccgttt
    10441 tacacctcaa ttatggagcc ttgctaagtg gggaacacct ttaaatcaat ccttgtctgg
    10501 gtcgccttgg gtatcggcct gatggctgcg ttcaacgctt tagacggtaa aaaagaagac
    10561 aacgggcaaa tcgaatattc tcagttcatc caacaggtca acaacggcga agtatccggc
    10621 gtcaacatcg aaggatccgt cgtcagcggc tacctgatta agggcgagcg caccgacaaa
    10681 agcaccttct tcaccaacgc gcctttggac gacaacctga ttaaaacact gctcgacaaa
    10741 aacgtccgtg taaaagtaac gccggaagaa aaaccgagcg cgctggctgc cctgttttac
    10801 agcctgctgc ccgtcctgct gctgattggc gcgtggttct actttatgcg tatgcagacg
    10861 ggcggcggcg gaaaaggcgg cgcattctca ttcggcaaaa gccgcgcccg cctactggac
    10921 aaagatgcca acaaagttac ctttgccgat gtcgccggct gcgacgaagc caaagaagaa
    10981 gtgcaggaaa tcgtcgatta cctcaaagcg ccgaaccgct atcaaagcct gggcgggcgc
    11041 gtgccgcgcg gcatcctgct ggcgggcagc ccgggtacgg gtaagacgct tttggcgaaa
    11101 gcgattgcag gcgaagccgg cgtgccgttc ttcagcattt caggttccga ctttgtcgaa
    11161 atgttcgtcg gtgtcggtgc aagccgcgtc cgcgatatgt tcgagcaggc gaagaaaaac
    11221 gccccctgca tcatctttat cgacgagatt gacgcagtcg gccgccaacg cggcgcaggt
    11281 ttgggcggcg gcaatgatga gcgcgagcaa acattaaacc aattgttggt tgaaatggac
    11341 ggttttgaga gcaatcagac tgtaattgtg attgcggcaa ccaaccgccc cgacgtactc
    11401 gatcctgcgc tgcaacgccc cggccgtttc gaccgccaag tggttgtccc cctgccggac
    11461 atccgggggc gcgaacagat tttgaacgtc cattctaaaa aagtgccttt ggacaaatct
    11521 gtggatttat tgtccctcgc gcgcggcacg ccgggttttt ccggcgcgga tttggcgaac
    11581 ttggtcaacg aagccgccct gtttgccggc cgccgcaata aagtcaaagt cgatcagagc
    11641 gattttgaag acgccaaaga caaaatctat atgggtccgg aacgccgcag tatggtgatg
    11701 cacgaagacg aaaaacgtgc gacggcgtat cacgaatccg gacacgcgat tgttgccgaa
    11761 agcctgccct ttaccgaccc cgtccacaaa gtaaccatta tgccgcgcgg acgtgcgctg
    11821 ggtctgactt ggcagcttcc ggagcgcgac cgcatcagta tgtataaaga tcagatgttg
    11881 agccagctct ccatcctgtt cggcggacgg attgccgaag acatcttcgt cggacgcatc
    11941 tccaccggcg catcaaacga ctttgaacgc gcaacccaaa tggcgcgcga aatggtaacg
    12001 cgctacggca tgagcgacaa aatgggcgtg atggtttatg cggaaaacga aggcgaagtc
    12061 ttcttgggac gcagcgtaac ccgttcgcaa aacatttccg agaaaaccca gcaggacatc
    12121 gacgcggaaa tccgccggat tttggacgag caatatcagg ttgcctacaa aatcctcgat
    12181 gaaaaccgcg acaagatgga aacgatgtgc aaagccctga tggaatggga aaccatcgac
    12241 cgcgatcagg tactggaaat catggcgggc aaacaaccca gtccgcccaa ggattacagc
    12301 cacaacctgc gcgagaatgc ggacgcggcg gaagataacg cgccgcacgc tccgactcgg
    12361 gaagaaaccg aagcacctgc cccggcagac accgcttcga cagagtccga gcagcagcct
    12421 gaaaacaagg cttaacttcc cgaacaaacg gcagcccgca agctgccgtt ttcccatcct
    12481 gttttcagac ggcattttcc actcaatgcc gtctgaaacg ccaaagccgt atcggttttt
    12541 acgaatctgc gatatggcgg tttaaattga aatcagaacg gggcaaggct gtaccggttt
    12601 aaaattaaac cgctataata ccgcccttcg aatacacgcc cgcattggtg atgcccgtga
    12661 aaaaagttga aaaaaacatc ttggtgctgc acggcgcgga caaaatgttc gagctggtcg
    12721 acaaggttga ggattacccg cactttctgc cgtggtatag caaaaccgag gtcatcggac
    12781 gtagcggcaa cgaactgaag gcacggctgt ttatggacta tatgcacgtt cgccaatcgt
    12841 ttgccacgca caaccgcaac attccgggca gggaaatccg tatggaactg ctcgagggtc
    12901 cgttcaaaac cttacgtgga acgtggaaat ttatcgattt gggcgacgat atgtgcaaaa
    12961 tcgaattcaa tttggaatac gatttttcca atgccgtttt gtccgcctta atttcccccg
    13021 tcttcaacca cctttccgcc acgctggtcg aagcgttcgt caaagaggca gaccgccgtt
    13081 atgcttgaaa ttgagattgt gtacgggctg cccgaccgac aggttttgaa aaccatgcag
    13141 cttgccgagg gaacaaccgt ccgcgccgcc gcactgcaaa gcggtttgga cggcatattt
    13201 gaagatttaa acctgcattc cgcgcctttg ggcattttcg gcaaagccgt caaagacgac
    13261 acgcccctgc gcgacggcga ccgcatcgaa gtgtaccgcc cgctgttgat cgatcccaaa
    13321 gaagcgcgcc gcaaacgcgt tcaaaatcaa gaagaataac catgccgtct gaagccttca
    13381 gacagcattc gacagaaaca cggaaaatat catgtcaaac aaaatcaaaa tggttgtcgg
    13441 cttgggcaac cccggcaaag aatacgaaca aacccgacac aatgcgggtt tttggttcct
    13501 cgacgaactg gcgtggaaat ggaaggcttc atttaaagaa gaaaaaaaat tcttcggcga
    13561 agtcgcccgt gccgccctgc ccgacggcga cgtttggctg ctcaaacctg ccacgttcat
    13621 gaaccgttcc ggacaggcag ttgccgcgct tgcacagttc tacaaaatca aacccgaaga
    13681 aatcctcgtc gtccacgacg aactcgacat tccctgcgga cgaatcaaat tcaaactcgg
    13741 cggcggcaac ggcggacaca acggcttgaa agacattcag gcaaaactcg gcacggcaga
    13801 ctattaccgc ctgcgcctcg gcatcggcca cccgggcgac cgcaacctcg tcgtcggcta
    13861 tgtcctgaac aaacccagta cggaacaccg ccgacagatt gacgatgccg tcgccaaatc
    13921 cctgcaagcc atacccgaca tccttgccgg caaatgggaa gaagcaaccc gcttcctgca
    13981 cagcaaatga cccgatgccg tctgaagccc tttcagacgg catgttcccg atttccatat
    14041 ccgaacagtc atgaccgaac tcaagcagct tatccaaacc gaatccatcc ccgtcatcga
    14101 agaaaccctc gatttcctgc tgtacgaatg cagcatcgac gacgcaccat ccgccgaaga
    14161 agtggcacaa tggcgcgaca tacttgccgc acgcggcggc aaattcctgc gcctgtccaa
    14221 aatctgccaa acgtggctgg acgaggaggc ggcatgaagc tgccgcgcaa ccgcttcagc
    14281 ctgctttccg cattgtggtt tgccggcggc atctattcgc tgctcttcaa agctgccgac
    14341 accgcgccgc cgccgtttcc gcatttcgac aaagcagcac accttgccct gtttttcgca
    14401 caaatctggc ttttgaccaa agcattcaaa accggaaaac ttcccatccc ctaccgcagc
    14461 ctgatggtct ttgccctctg tttcgccctc ttcagcgaat gcgcgcaggc atggtttacc
    14521 gcaacgagaa ccggcagttt gggcgatgtt cttgccgata tggcaggtac ggttctcgca
    14581 ctctttgccg cccgcgccgc cgaccgcccg gactgaatcg gtttatttcc cccaaacagg
    14641 atgcacctct tcccgataca tcctattttt ccccttacat atcaatccca tctcataaac
    14701 aaaaccgaca aatcgtttac aatatattta cactacatca gattacaaat atactcgaac
    14761 cagttgcaga atcggcggtt ccacaaaccg tttcggattg cacgacacaa aagaaaaacc
    14821 gattttattg ccttaaagga gtattcatga acctgcatgc aaaggacaaa acccagcatc
    14881 ccgaaaacgt cgagctgctc agtgcgcaga agccgattac cgactttaag ggcctgctga
    14941 ccaccattat ttccgccgtc gtctgtttcg gcatttacca catcctgcct tacagccccg
    15001 atgccaataa aggtatcgcg ttgctgattt tcgttgccgc actttggttt accgaagccg
    15061 tccacattac cgttaccgcg ctgatgatcc ctatcctcgc cgtcgtactc ggtttccccg
    15121 acatggacat caaaaaggcg atggctgatt tttccaaccc gattatctac atttttttcg
    15181 gcggtttcgc actcgccacc gccctgcata tgcagcgtct cgaccgcaaa atcgccgtca
    15241 gcctgctgcg gctgtcgcgc ggcagtatga aggccgccgt gctgatgttg ttcctcgtta
    15301 ccgcctttct gtccatgtgg atcagcaaca ccgccaccgc cgcgatgatg ctgcctctgg
    15361 caatgggtat gctgagccac ctcgaccggg aaaaagaacg caaaacctac gtcttcgtcc
    15421 tgctcggcat cgcctattgc gccagcatcg gcggcttggg cacgctcgtc ggctcgccgc
    15481 ccaacctgat tgccgccaaa gccctaaatc tggacttcgt cggctggatg aagctcggcc
    15541 tgccgatgat gctgttgatt ctgcccttga tgctgctctc cctgtacgtc atcctcaaac
    15601 ctaatttgaa cgaacgcgtg gaaatcaaag ccgaatccat cccttggacg ctgcaccgcg
    15661 tgatcgcgct gttgattttc cttgccacag ccgccgcgtg gatattcagc tccaaaatca
    15721 aaaccgcctt cggcatttcc aatcccgaca ccgttatcgc cctgagtgcc gccgtcgccg
    15781 tcgtcgtctt cggcgtggcg caatggaagg aagtcgcccg caataccgac tggggcgtgt
    15841 tgatgctctt cggcggcggc atcagcctga gcacgctgtt gaaaacatcc ggcgcgtccg
    15901 aagccttggg acagcaggtt gccgccacct tttccagcgc gcccgcattt ttggtgatac
    15961 tcatcgtcgc cgccttcatt atttttctga ccgagttcac cagcaacacc gcctccgccg
    16021 cattgcttgt accgattttc tccggcatcg ctatgcagat ggggctgccc gaacaagtct
    16081 tggtattcgt catcggcatc ggcgcatctt gtgccttcat gctgccggtt gccacaccgc
    16141 ctaacgcgat tgtgttcggc acaggcttaa tcaagcaacg cgaaatgatg aatgtcggca
    16201 tactgctgaa catcctctgc gtagtattgg ttgctctgtg ggcttatgct gtactgatgt
    16261 aaaccatcga cctaaacaac aagaccgtct gaaagaatat ttttcagacg gccttgaagt
    16321 ttgtcgttca gacacaattt gtcgaatcat tcaaaaccgg attctaacga aaggaaaccc
    16381 atgattatcc tgcacaccaa caaaggcgac atcaaaatcg aactcgattt cgacaaagcc
    16441 cccgttaccg ccaaaaactt cgagcaatac gtcaaagacg gcttctacga cggcgtaatc
    16501 ttccaccgcg tcatcaaagg cttcatgatt caaggcggcg gcatggatga aaacatgaac
    16561 gaaaaagaaa cccgcgatcc gattcaaaac gaagcgtcca acggcctgcc caacgataaa
    16621 tacaccatcg ccatggcacg cacttccgac ccccattccg ccagcgcgca attcttcatc
    16681 aacactgccg acaacgcttt cctgaacttc cgttctaaag agctgtacgg caaaaccgtc
    16741 gtccaagact ggggctatgc agtattcggt aaagtcgttg acggttttga cgttgtcgat
    16801 gccatcgaag gcgtatccac caaacgccac ggctaccatg acgacgtacc gagcgaacct
    16861 gtcgtcatca ttaaagccga agcggtataa accggcaatc cgaaagcagc ctgcacaaag
    16921 gctgcttttt ttgtttcagg cggcataaaa tccaagcaca tatgccgtct gaacacctcc
    16981 cgcaagcctt cggacaatgt tgcaacgtgt taaaatagcc tcctttcctg aaaccacaac
    17041 aaggaaacca tcatggcaaa tattgcccgc gacatcttca aagcctacga catccgaggc
    17101 attgtcggca aaaccctgac tgacgatgcc gcctatctca tcggcagggc catcgccgcc
    17161 aaagccgccg aaaaaggcat gacccgcatc gcgctcggac gcgacggacg cttgagcggt
    17221 cccgaactga tggagcacat ccaacgcggc tttaccgaca gcggcatcgg cgtactcaac
    17281 gtcggcatgg tcgccactcc tatgctctat tttgccgcca tcaacgaatg cggcggcagc
    17341 ggcgtgatga ttaccggcag ccacaatccg cctgattaca acggtttcaa aatgatgctc
    17401 ggcggcgaca cgctcgcagg cgaagccatt caagaacttt tagctattgt tgagaaagat
    17461 ggttttgttg ccgccgacaa acaaggcaac gtaaccgaaa aagacatctc cggcgaatac
    17521 cacaaccaca tcgtcggcca catcaaactc aaacgcccca tgaaaattgc catcgacgcg
    17581 ggcaacggcg tgggcggcgc gtttgccggc aaactctata aaggcttggg caacgaagta
    17641 accgagcttt tctgcgacgt ggacggcact ttccccaacc accatcccga cccatccaaa
    17701 ccgaaaaacc tgcaagattt gattgccgcg ctgaaaaacg gtgatgccga aatcggcctg
    17761 gcgtttgacg gcgatgccga ccgcttgggt gtggtcacca aagacggcaa cattatttat
    17821 cccgaccgcc aactgatgct gttcgcccaa gacgttttga accgcaatcc cggcgcgaaa
    17881 gtcattttcg acgtgaagtc cacccgcctg cttgcgcctt ggattaaaga acacggcggc
    17941 gaagccataa tggaaaaaac cggccacagc tttatcaaat ccgccatgaa aaaaaccggc
    18001 gcgctggttg ccggcgaaat gagcggacac atcttcttca aagaacgctg gttcggcttc
    18061 gacgacggtc tgtacgccgg cgcacgcctc ttggaaatcc tgtctgcctc cgataatccg
    18121 tccgaagtgt taaacaacct gccgcaaagc atttccacgc ccgaactcaa catcgccctg
    18181 cccgaaggca gcaacggcca tcaggttatc gacgaactcg ccgccaaagc cgaatttgaa
    18241 ggcgcaaccg aaatcatcac catcgacggt ctgcgcgttg aatttcccga cggcttcggt
    18301 ctgatgcgtg cttccaatac cacgccgatt ctagtgttgc gttttgaggc ggatacgcaa
    18361 gcagccatcg agcggattca aaaccaattc aaagccgtca tcgaaagcaa tccaaattta
    18421 agctggccgc tgtaaacaca ggaaaaatgc ttaatcaaat cttggtagat tggatttggc
    18481 ttaaaaatct ccgcgtccgt tcgtgtttgg ggtggtcgaa caactctttc gggctgccct
    18541 gctctacgat aacgccgccg tccatgacga cgacggtcgt ggcaacttcc agcgcgaact
    18601 tgatttcgtg ggtaacgacg accatcgtcc aaccttcccg cgccaattcc ttcatggtat
    18661 ccaaaacatc ttgcaccaat tcagggtcga gcgcggaagt cggttcgtca aacaacatca
    18721 gctcgggctg aatcgccaat gcgcgggcaa tgccgacgcg ctgctgctga ccgccggaaa
    18781 gctggtaggg atacaaatcc actttgtcgc ccaagccgac tttttccagc agtttcagag
    18841 cctcttcgcg cgcttgggcg gcaggcttgc cctgtacggc aaccggtcct tccattacat
    18901 tttccaaggc ggttttgtgc ggaaagaggt tgtattgttg aaacaccatg cctgatttgc
    18961 ggcgcagtgc caaaatatcg tgtttgcttg gttttttaga aaaatcgatt ttcagcggtc
    19021 gctcgttgtc gaactcgatt tgtccgtctt cgggcatttc caacgcgttt aggcatcgca
    19081 gaaacgtcgt tttgcctgag ccggaaggcc cgaggataac gaccacctgc cctttgccca
    19141 catccaaatc gatgccgcgc aaaatagtgt tttcgccgaa ggtcttatgg atattgcgga
    19201 ttttaatcat aacaactcct tatttggcga cataacggtc gaaacgtttt tccaaacgtg
    19261 cctgaatcag gaacagaacc ttgcagaaac accagtaaac caatgcggct tcgatataga
    19321 cgggcaaaaa gtcgtaagtg cggtttgccg tttcctgcgc gacgcggaac aattcagtta
    19381 ccgttaccac cgctgcgagc gaggtgtttt tgaacaagcc gataaactcg ttgctcaaag
    19441 gcggcacggc aacgcggaat gcctgcggcg cgacgatgcg gcggaacgtc tgcatatagg
    19501 tcataccgat ggagaaacct gcttcccatt ggcctttagg tacggacaaa attgccgcgc
    19561 gtatggtttc ggaagcgtat gcaccgacat tgagcgagaa gccgataatg gcggcgggaa
    19621 tcggatcgat atagataccg acggacggca gcccgtaaaa cacaatcaca agctgcacca
    19681 acagcggcgt accgcgaatg acggaaatat aaaattccac caatttcagc aggattttcc
    19741 gcacgatgcc gccggcgggc ataatccgca ccaaagccac ggctaccgca atcatcatac
    19801 cgataacgaa agaagctgcc gccaaaggca gagacaccgc gaagccggct ttgaccatag
    19861 gcaaaaacgc gctgacaatc atatcggcgc gtgtttccgt cataaacggc agcgaagcaa
    19921 ggaaattatt gaacactgat gtcttttccg aagaattgtt cgcccagttt tttcagcgta
    19981 ccgtcggctt tcagctcgtt gattgccgta ctgaatttcg ccacggcttc gtcattgccc
    20041 ttgttgacaa tcaggccgga accgactttt tcatcggcag gtgcggacca aacgattttc
    20101 acgcccgcat tcgggttttt cttcagatag tccaaaaccg ccaattcgtc gttcagggtt
    20161 gcatcggcac gtttttgttc aatcagggtc agcgattgcg ccaaaccgtc aacagccacc
    20221 aaatctgcgc ctgcagcttt ggctttttcg ccgtagttgc tggtcaggga ttgtgcggtt
    20281 ttcacgcctt tgatgtcgtc gatagatttg atgttgctgt cgttacgggc aaccaatacg
    20341 gcaccgctcc agctgtaagg atcggatttg tcgaatgtcg cttggcgttc ggggctggtc
    20401 agaccgactt ggtttgccac cacgtcgaaa cgccccgcct tcaaacccgc catcatcgaa
    20461 tcccattgcg tttctttaaa ctcgactttc acgcccagtt tttccgccac ggcgcgggtt
    20521 acttccacat cgtaaccggt cagtttgccg tccttgtcgt ggtaggtaaa cggtgcgtaa
    20581 gtgccttccg tgccgacggt aaccgtgcct ttattgttga tgcgctcgat taaagaaccg
    20641 gaaactgccg attgtgcagg cacggaagat gctccgctgc cgccttccga accaccgcag
    20701 gccgccaaaa ccaatgcggc aataccgccg agtacgaatt ttttcaacat cattctctcc
    20761 tgtaaaaagt gtgcaagtgc ggcattctaa gtgaaattgc ggcaaacaca aagaatactt
    20821 tttttatatg gttattccaa atcggaatgg aaaagatatg ccgaagccgc cggaagcgga
    20881 aataaagggg gcgggaaagg cggcgggcgg cacgaaaccg aaaagcggca tcaggtatgc
    20941 aaacggcgga ttcacgcacg ccgttccggc aaaaatccgc ctgccgattc ggtgctataa
    21001 tccatccgaa acagatgaca ccaaaaagga acatctcatg aacacgcccc aatccgccat
    21061 cattcccgac cacgcccaag ccggcatctt tatcgaagcc gacttcgccg ccaaccgcct
    21121 caacgatatt aaagccgcct gccgcgcttc gctcgacgcg ttaactgcct tgaaagcccg
    21181 ctttccggac gatattttgg gtctgaccat cgccttcggc agcaaagcct gggcaacatt
    21241 cggacatacg gacgaaggca gtgaaatcaa acccttctcc gaaatgggca acgggcttgc
    21301 gccgtccacg cagcacgata tgtccatcca catccaatcc ttccgccaaa acgccgccta
    21361 cgcgcttgcc caatccgttt tgggcgcgtt cggcgacagc atatgcgtcg cgtccgaaga
    21421 acacggtttg cgcctgtatc aggatcgcgg actggacggt ttcgtcgacg gcacggaaaa
    21481 cccgcagggc gatgaaaatg tccgcgaagt cgccatcatc cccgaaggaa aacccgatgc
    21541 gggcggcagc tatgtcctgc tgcaaaaata cctgcacgat ttgaaaaaat gggatgccgt
    21601 ccccgtcgcc gaacaggagg cctcggtcgg acgcggcaag gaaaccgacg acgaattcgg
    21661 ccgcgatgtc cgcctgcccg attcgcacct cggccgcgtc aacctgaaag aaaacggagt
    21721 cggcctgaaa atcgtccgcc gcagcctgcc cttcggcaaa atcagcggcg aacacggttt
    21781 gatgtttacc gcatactgcc gcactctgca caatatcgaa gcgcaattat tgagtatgtt
    21841 cggcgataca gacggcaaaa ccgacctgct gctccgacac ctctccgccg ccgtttcggg
    21901 cggatactac tacgcgccgt ctgtcgaacg cctgcaaaac ctctaagccg gacgatgccg
    21961 tctgaaacca tttttcagac ggcatttt
//
LOCUS       DAXHSJ010000018        21316 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000018 DAXHSJ010000000
VERSION     DAXHSJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21316
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.018"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..1005
                     /locus_tag="ACFM8U_000497"
     CDS             <1..1005
                     /locus_tag="ACFM8U_000497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333786.1"
                     /translation="NLIHYKIESSQIKAHILAHPDYAAFKAGHLAKFAAWHTQNDLAV
                     IQPGSRPGRLIRKWSESLLDAFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWK
                     AVKNLAEITKESDEAANLTVVFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFS
                     DGIAKLEEKQSELERLSQELENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGI
                     EEGERAVLQTTQTLMTQEKAAKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWL
                     ADLQSRIENRLENSIQQLISRLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG
                     "
     gene            1156..1281
                     /locus_tag="ACFM8U_000498"
     CDS             1156..1281
                     /locus_tag="ACFM8U_000498"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333787.1"
                     /translation="MLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT"
     gene            complement(1571..2086)
                     /locus_tag="ACFM8U_000499"
     CDS             complement(1571..2086)
                     /locus_tag="ACFM8U_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333788.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(2552..4474)
                     /locus_tag="ACFM8U_000500"
     CDS             complement(2552..4474)
                     /locus_tag="ACFM8U_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6333789.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFAGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(4551..4922)
                     /locus_tag="ACFM8U_000501"
     CDS             complement(4551..4922)
                     /locus_tag="ACFM8U_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC6333790.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLARQSFHKPVLVHKDSVCLSQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIGVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            5119..6426
                     /locus_tag="ACFM8U_000502"
     CDS             5119..6426
                     /locus_tag="ACFM8U_000502"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC6333791.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            6419..6853
                     /locus_tag="ACFM8U_000503"
     CDS             6419..6853
                     /locus_tag="ACFM8U_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333792.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(6912..7181)
                     /locus_tag="ACFM8U_000504"
     CDS             complement(6912..7181)
                     /locus_tag="ACFM8U_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC6333793.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(7363..9825)
                     /gene="lon"
                     /locus_tag="ACFM8U_000505"
     CDS             complement(7363..9825)
                     /gene="lon"
                     /locus_tag="ACFM8U_000505"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC6333794.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEADIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGNDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFSKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGVEAAAESASKPKPRSRATKH"
     gene            9925..10101
                     /locus_tag="ACFM8U_000506"
     CDS             9925..10101
                     /locus_tag="ACFM8U_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HFC6333795.1"
                     /translation="MPSETFWLQTAWAWKDKAGTKNCSVLPLLAVPSVWFTVLPPYFQ
                     NMTQVSHVFFYFSR"
     gene            10076..11179
                     /locus_tag="ACFM8U_000507"
     CDS             10076..11179
                     /locus_tag="ACFM8U_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6333796.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGADGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRSALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAQAPSTGFQTALDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPRAVACARANIARLGLEKQVEIRETDMFPDMRADLIVCNPPWLPAKPTSAVESALY
                     DPDSAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPIHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(11251..12996)
                     /gene="recD"
                     /locus_tag="ACFM8U_000508"
     CDS             complement(11251..12996)
                     /gene="recD"
                     /locus_tag="ACFM8U_000508"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC6333797.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTILDRETYKSLNLYLPEHGFPVHEDAPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNAQVERLYRAHKAYWQAVKDGN
                     IEAAYVGISDIVVLAAWRQDSEDFNEAYCRHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(13063..13758)
                     /gene="lolD"
                     /locus_tag="ACFM8U_000509"
     CDS             complement(13063..13758)
                     /gene="lolD"
                     /locus_tag="ACFM8U_000509"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC6333798.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEILAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLSQRQLGTLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAETAMAMLEKVGLKHRSRHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGCFGRVMVM
                     KDGSLHPKQGANA"
     gene            complement(13751..14998)
                     /locus_tag="ACFM8U_000510"
     CDS             complement(13751..14998)
                     /locus_tag="ACFM8U_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC6333799.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYNNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            15203..15481
                     /locus_tag="ACFM8U_000511"
     CDS             15203..15481
                     /locus_tag="ACFM8U_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333800.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(15537..16154)
                     /gene="recR"
                     /locus_tag="ACFM8U_000512"
     CDS             complement(15537..16154)
                     /gene="recR"
                     /locus_tag="ACFM8U_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC6333801.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(16221..17759)
                     /locus_tag="ACFM8U_000513"
     CDS             complement(16221..17759)
                     /locus_tag="ACFM8U_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC6333802.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGVDYIVQVGD
                     EKISEQSLNAAVQNEQAGGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPTNEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(17819..18193)
                     /locus_tag="ACFM8U_000514"
     CDS             complement(17819..18193)
                     /locus_tag="ACFM8U_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC6333803.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKALFSTVEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            18343..19971
                     /locus_tag="ACFM8U_000515"
     CDS             18343..19971
                     /locus_tag="ACFM8U_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC6333804.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTTGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNATMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            20043..21296
                     /locus_tag="ACFM8U_000516"
     CDS             20043..21296
                     /locus_tag="ACFM8U_000516"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC6333805.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVRKVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQVDSGKIAAECWAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT         4853 a         5756 c         5579 g         5128 t
ORIGIN      
        1 aatttaatcc actataaaat cgaaagcagc caaatcaaag cccatatatt ggcgcacccc
       61 gattacgccg ccttcaaagc cggacacctg gcaaagtttg ccgcgtggca cactcaaaac
      121 gaccttgccg tcatccaacc gggcagccgg ccgggcaggc ttatccggaa atggagcgaa
      181 agcctgctgg acgcgttcaa acccggcagc ctgattgaag aatacgattt ctaccaaatc
      241 ctgacggact actgggcgga aaccctgcaa gacgatgttt atctcatcgc ccaagatggc
      301 tggaaggcgg ttaaaaacct ggccgaaatc accaaagaaa gcgatgaagc cgcgaacctg
      361 accgtcgtct ttgaggaaac cgaaaccggc aaaaaaggca aagccaaaac caagcgcatc
      421 agcaaaaaat accgcagcga agtcatcgcc cccgagctgg ttgcccgccg ctacttttca
      481 gacggcatcg ccaagctgga agaaaaacaa agcgagctgg aacgcctaag ccaagaattg
      541 gaaaaccaca tagaagaaca cggcggcgaa gagggtgcgc tgaacgacgt attggatgca
      601 aaaggcaaac tttccgccaa acttctgaaa accgcattgg aagaaagcgg catagaagaa
      661 ggcgaacggg ctgttttaca aaccacccaa acactgatga cgcaggaaaa agccgcgaaa
      721 gacgcagtca aaacccaaat cgaagccctg aaccttgccg tattcaaaca attcggccga
      781 ctttccgaag ccgaaatcaa gcagcttgcc gttcaagaca aatggcttgc cgatttacaa
      841 agccgaatcg aaaatcgctt ggaaaacagt attcagcagc ttatcagccg cttgaacacg
      901 ctggaagacc gctaccgcag cccgatggcc gagcttgccc gagaagtgga aaagtggcaa
      961 agcaaagtca atgcccacct tgaaaatatg ggttttggag gctgaaatgg cggcacagac
     1021 aggctataag gcgagcgggt tttgagacct ttgcaaaatt ccccaaaatc ccctaaattc
     1081 ccaccaagac atttagggga tcgcggttcg ggtgtccgca ccgcttaata cgtcgtcgtc
     1141 cacgaactga cccatttgct cgaacgccat cgcaacgccc gttttatggc gcatatggac
     1201 aactttctcc caaactggca aagcatcaaa caacagctta atgccttgga gttatttgca
     1261 caaatatata atttaacata atatacatta tgcgaactat cggaaacagt tgtacgtgtc
     1321 cctgtggtct ttccaagtag gaaaattaaa gtatggccaa tgcggctgaa tgtatagccc
     1381 ggagcatccg cgtatccgaa ggtactgaac gacatgccct gcatcccttt cgatgcttcc
     1441 atccagccgg tattgggaaa tacgttgacc ttcaatataa tatacaggca gccgacacac
     1501 aggatatgcc gctgcttttt catcatttct tctgtcaaat ccttggaacg gtcgatttga
     1561 aaacacggct ttacatacgt cccaattctc tttgcagtgc ttggatattt tgctgcctgt
     1621 ccaatacatt gctttgtaat gcatttattt cttgatggtt gatgttgccg ccctttgcca
     1681 gacgtgcttg tgataacatt ttttgggctt cagtcagggc tttgcgttcg ttgctcaatt
     1741 ctgcttcgag aatggagcgt ctgctgttgt ttacaggtgc ttgttgaggc gggggcgtat
     1801 tggattttgc cggcttggat actgttttga ccggggcttt atatttgaca gcctgtccgc
     1861 cgtttgacgg tgatggtgtc ggttcggatg tttggggcgg gatatagcgt tcgctgctgt
     1921 agttgccgat tgggggcaaa tcggttgaga ggcagctttt ggacggcttg gtggtgtaaa
     1981 cggtttctcc gttgattgtg caggtgtaga ttttggccgc attcgcaccc aatgggcttg
     2041 aaatcaggga aaagttgatt aggattaagg ggagttttga tttcataatg tgcttgatgc
     2101 tcggataatt atttgatttt ttggtatttt ttgtaatgct ttcttggcgt tttacgggct
     2161 tttcttttcg gttggttgct tttgattttc tttcggggct ttggcagcct gcttgttctt
     2221 tttcccggca ggttttttgt ttttcttttt actttctatg tgtattttgg cttcttaact
     2281 gagtttttta attttcaggc ggtatccgcc tccctgatgg ctgctgattt taggtaaatc
     2341 cgcatcggcg cacgatcctg ctggggctga tgtatatacg cttttgccat ttgagttgca
     2401 atggtatacg gaggcttgcg ccgccgcgcc ggaaagggac aggagaccca aggcggcaaa
     2461 aagtctgatg tttataccgg taaaaggcgg tgataacgcc cgaattatac cgttattggc
     2521 aggcaaagat aagcaccctg cccgcgcttc tttatcgctc ggcaaactgt ttctcgattt
     2581 cagtttcaat ccgttccaat tcttcttgac aagccagcca aacctcttcg atttgggcaa
     2641 gttgtgtttt gacttttgcc agctgggata aggtattttg caatttttct ttgtttgctt
     2701 caaggtaagc gtcttcttgt gctaaaaatg cttcacatgc cgtctgaatt tcggaaagct
     2761 gcgccatttc tttttcggca cggtctattt tctgctgtat cggcttgccg cgtcgggctt
     2821 tttcctgacg gatttgcgct tcgatgcgct tggtgtcttt gcggctttgg ctttgtgcgg
     2881 atgctgcggg tgcggcggcg gcgttttcct gtgccaaacg ccattggcgg taatcgttca
     2941 aatcgccgtc gaagttcttc agacggcctt tgtcgatcag gaggaagctg tcggtagtcg
     3001 cttcaagcag gctgcggtcg tgcgatacga tgattaaggc gccttggaaa ctttgcagag
     3061 cgagcgtcag ggcgtggcgc atatccaagt ccagatggtt ggttggttcg tcaagcagca
     3121 gcaggttcgg cttttgccag ataatcatgg caagggcgag acgggctttt tctccgccgg
     3181 aaaaaggttc ggttttctgc aacgccatat cgccggcaaa gttgaagccg ccgaggaagt
     3241 ttcggatttc ttgttcgcgt acttcgggag aaagctgctg gatatgccaa acagggtttt
     3301 ggtcggcgcg gatggtatcg agttggtgtt gggcaaaata gccgatattg agtttttcgg
     3361 aacggacgat gctgccggaa agcaaatcga ttttgcccgc caaggctttg ataaaggtag
     3421 atttaccgct gccgttgaca cccaatagcc cgtagcgtgc gccgctttcc agcgacaggg
     3481 taatgtcatg cagaacggtt ttgccttcgt aacccaaatc agcgtgttct agctttaaca
     3541 aaggattggg cagatggtcg ggatggtaaa actcaaagga aaactcgctg tccagatgcg
     3601 cgggcgcaac gcgttctagc tttgccaaag ccttcatgcg gctttgcgct tgaacggctt
     3661 tggtggcttt ggctttgaag cggtcgataa aggattgcag atgtttgatt tgcgcctgct
     3721 gtttgacata ggcagcttgt tgttgcgcga gacgctgcgc gcgttcgttt tggtaaaaat
     3781 cgtaattgcc gccgtattgc gtgagttttt gctgcgataa ttcaatggtt tgggtagttg
     3841 ccgcgttgag aaaatcgcgg tcgtgggaaa tgatgatttg cgtgcagggt aaagaagcaa
     3901 gatggttttc cagccacaag acggtttcca aatccaagtg gttggtcggt tcgtccaaaa
     3961 gcagcaaatc ggcgcggcaa atcagggctt gggcgagatt gaggcgcata cgccagccgc
     4021 cggaaaagga tttgacgggg cggctgtgtt cttcttgcga aaaacccagc ccgttcaaca
     4081 attttgccgc gcgggccggc gcggtataag cgtcgatttc ttccaatttg gcatgatatt
     4141 ccgcctgctt catgccgtca ttttgcgctt ccgcctgcct caatgccgtc tgaaaagcct
     4201 gcaactcggc atcgccctgc aaaacgtaat ccaaagcgga aatatccaaa tcgggcgttt
     4261 cttgggaaac ggaagcgagc cgccagtttt tcggaatcga gacatcgccg ccgtcctgag
     4321 tgattttacc cttgattaag gcaaacaggc tcgatttgcc cgttccgttt ttgccgatca
     4381 aaccgacgcg ctgaccggga ttgacggtag cgttggcttt gtcgagcagg actttcaaac
     4441 cgcgttgcag ggtgaggttt ttgatttcaa tcataacgga aacatcgtcg ggcgggaaaa
     4501 gcccgtattt tacctgaaag tcagtgccga tgccgtctga aacgggaaat ttacggctga
     4561 agccaagccc aagccctgcg cccttccgag tgcaggaaaa ccaatgtcct gaatgccgaa
     4621 tcggtattca tacattccac gccgattccg attcgggaaa aatccgccat gattttggga
     4681 tggataaact cctgagccgc gcccgtcccg ataatcaata tttccggata gtcaacaggt
     4741 ttgatgtcgg acaacaggtt ttccggagtc agatcggaca aggttcggca ttgcgacagg
     4801 cagaccgaat ccttatgtac aagcacgggt ttatggaaac tttgcctcgc cagccggatt
     4861 ccgcccgcac cgcattcata ttccgcaaac tgtccgtcta tcggattttc ttcaaacaac
     4921 atttttttac cccgtcgccg catcatctac accgaaaggg gtgcaaaatc agacaaattc
     4981 atgtaggatt ggcagatttc atctgacccg cctgccgatt tcagacggca tttgattcaa
     5041 agtgcggcac aattatatcg gcagcggata ttttcgtctt tcaatattta catttcagtc
     5101 ggcttacaag gagacacaat gaagccagta aacatcggtc ttttaggttt gggtacggtc
     5161 ggcggcggta cggctgccgt gttgcgggac aacgcggagg aaatttcccg ccgcttgggg
     5221 cgcgaaatcc gtatttctgc cgtgtgcgat ttgagtgaag aaaaagcccg acaaacctgc
     5281 ccgtccgcag cctttgtcaa agatccgttc gaactggtcg cacgtgaaga cgtcgatgtc
     5341 gtcgtcgaat tgttcggcgg taccggcatt gccaaagatg cggtgttgaa agccattgaa
     5401 aacggcaaac acatcgttac cgccaacaaa aaactgctcg ccgaatacgg caacgaaatc
     5461 ttcccgctgg cggaaaaaca aaacgtcatc gtccaatttg aagcggcagt agcgggcggt
     5521 atcccaatca tcaaagccct gcgcgaaggt ttggcggcaa acaggattaa atccatcgcc
     5581 ggcattatta acggcaccag caacttcatc ctctccgaaa tgcgcgaaaa aggcagcgcg
     5641 tttgccgatg tactgaaaga agcgcaggca ttgggttatg ccgaagccga tccgaccttc
     5701 gacatcgaag gcaacgatgc gggccataaa atcaccatca tgagcgcact ggcattcggc
     5761 acgccgatga acttttccgc ctgctacctc gaaggcatca gcaaactcga cagccgcgac
     5821 atcaaatacg ccgaagaact tggctatcgc atcaaactgt tgggcattac ccgcaaaacc
     5881 ggcaaaggca tcgagctgcg cgtccaccct accctgattc ccgaaagccg cctcttggca
     5941 aacgtcaacg gcgtgatgaa cgccgtgcgc gtcaacgccg atatggttgg cgaaacctta
     6001 tattacggcg cgggcgcggg cgcattgccg accgcttccg ccgtggttgc cgatatcatc
     6061 gacatcgccc gcctggttga agccgatacc gcccaccgcg taccgcatct ggcgttccaa
     6121 cccgcgcaag tccaagcgca aaccatcctg cctatggacg aaattaccag cagctactac
     6181 ctgcgcgtcc aagccaaaga cgaaccgggc acgctggggc aaatcgccgc gctgttggca
     6241 caagaaaacg tctccatcga agcactgatt caaaaaggcg tgattgatca gaccactgcc
     6301 gaaatcgtga ttctgaccca cagcacggtc gaaaaacaca tcaagtcggc aatcgcagcc
     6361 atcgaagcac tggattgcgt ggaaaaaccg attaccatga tccgcatgga aagcctgcat
     6421 gactgagccg aaacacgaaa tgccgacgga agagcaggtt gccgcgcgca aaaaagcaaa
     6481 agccaaaatc cgcaccatcc gcatttgggc atgggtcatt ttggcgttgc tcgctttaac
     6541 cgccctgctc tcccaatgcg cgatgtccaa accgcaggca aaacagaaaa ttgtcgagtc
     6601 ttgcgtgaag aatattccgt ttgccgaaaa atggcaaaac gatttgcggg cccgcggttt
     6661 agattcaaac aatacccgcc tcgccgtcga ctactgcaaa tgtatgtggg agcagccttt
     6721 ggacagattg agcgagaaac agattagatc cttcggcaaa ctcggcgcac aagaacagct
     6781 tgacctgctc ggcggcgcaa atgcctttga agcacgtgac aagcagtgtg ttgccgattt
     6841 gaaatcagaa taatgtggac cgataaaaaa gccgattctt taaagaatcg gcttttttca
     6901 taaaaaacgg cttacagtgc gtctttcaat gctttacctg cacggaattt aggcgttttg
     6961 gcggcggcaa tggtcagagg ctcgccggtt ttggggttgc ggccttggcg ttccgcacgt
     7021 tcgcccacgt agaaagtacc gaaaccgacc aaagtaacgg tgtcgccttg tttcagggcg
     7081 gtggttactg cattggtagt ggcatccaaa gctttttgtg cggcggcttt ggaaatgtcg
     7141 gcttcttgag caatcgcttc gatcaattca gacttgttca caatcagtcc cttcctgtct
     7201 taaaaaatga tgaaatgccc gaatactcgg ggtttgtact gcttgagcaa ctttcgcttt
     7261 atagcaattc tgaaattgcc gtgtcaagca aaaaatacgg aatcacccta tttgacaggc
     7321 tttcaggacg aaaccgcatt tttacaacac atttcctgcg tttcaatgtt tggttgccct
     7381 gctgcggggt tttggttttg aagcggattc cgccgccgct tccacaccag aaggttctgc
     7441 ccaaggctca ggctggcttt ccaaacccag agccaatacc tcgtctatcc atttgaccgg
     7501 atggatggtc aggccggttt tcacgttttc agggatttct tccaagtctt tgacgttgtc
     7561 tttcggaatc aggacgtgtt tgatgccgcc gcgaagcgca gccaacagtt tttccttcag
     7621 accgccaatc ggcaggactt cgccgcgcag ggtaatttcg cccgtcatcg ctacatcggc
     7681 gcgtaccggg attttggaaa atgcagacac catcgccaaa gtcatggcaa tgcccgcact
     7741 cggaccgtcc ttcggcgttg caccttcggg aacatggaca tggatgtctt ttttctcgta
     7801 aaaatcagga gccaagccca ctgattccgc gcgggagcgg acaaccgacc atgctgcgga
     7861 cacggattcc ttcatcacat cgcccaactg gccggtgcac tgaatcacgc ccttacccgg
     7921 caatgctgcg gcttcgacgg tcagcagttc gccgccgact tctgtccacg ccagaccggt
     7981 tacttgtccg atacggtttt cgctttcggc aacgccgtaa tcgaagcggc gcacacccaa
     8041 atagtcgtgc agatttttct catttacttt aaccgcttta ggtttggctt tgctggtttt
     8101 cttggtttca gacaacctct tcttatcttc gtccaaggta atctgcatca ccaccttgcg
     8161 gcagattttg gcaatctcgc ggtcgagcga acgcacgccc gcctcgcggg tgtagtaacg
     8221 gataatatcg cgcaccgcgc tttcttcgat tgccaattcc ccttctttca cgccgttgcg
     8281 cttcatttgc ttcggcacga ggtactgcat cgcgatattg attttttcgt cttcggtata
     8341 gccggacaga cggatgattt ccatacggtc gagcagcggg gtcgggatat tcaggctgtt
     8401 tgaagtcgca ataaacatca catcgctcaa atcgtaatcc acttcggcat aatgatcggc
     8461 aaacttgttg ttttgttcgg gatcgagcac ttcgagcaac gcgctggcgg gatcgccccg
     8521 gaagtcgttg cccaatttgt cgatttcgtc gagcaggaac aatgggtttt ttacgccggc
     8581 ttttgccata ttctgcagaa tcttaccagg catagagccg atataggtgc ggcggtgtcc
     8641 ccggatttcg ctttcgtcgc gcacgccgcc caaagccatg cggacatatt tccgccccgt
     8701 tgctttggcg atggattcgc ccaaagaggt tttacctacg cccggagggc cgaccaggca
     8761 cagaatcggg cctttgagtt tgtccatacg tttttggacg gcgaggtatt ccaaaatccg
     8821 ttctttgact ttttccaggc cgtagtggtc ggcatccagc accagtccgg ctttggcgat
     8881 gtctttgctg acgcgggatt ttttcttcca cggcagctcg agcaaagtgt cgatgtagtt
     8941 gcgtacgacg gtggattccg cagacatcgg cggcatcatt ttgagctttt tcagttcgga
     9001 caggcatttt tcttccgctt ctttggtcat acccgccttt ttgatatctg cttccaaggc
     9061 atccagttcg ccgttttcgt cttcttcgcc cagttctttg tgtatcgctt taatctgttc
     9121 gttcagataa tattcgcgct gggatttttc catttggcgt ttgacgcgtc cgcgtatgcg
     9181 tttttcggcc tgcataatgt cgagttcgga ttccagctgt gccagcagga attccatccg
     9241 tttgccgatt tcgggaattt ccaaaatctg ttggcgttgc gccagtttca actgcaaatg
     9301 cgctgcgacc gtatcggtta gccggctgtt ttcggcaatg ccgttgatgc tgccgatgat
     9361 ttcggcgggg atttttttat tgagtttggc gtattgttca aactgcgcca acagggtgcg
     9421 gcgcacggct tcgaggtcgg tattgccgcc cgtgtcttct tccacgaccg cctctatatg
     9481 ggaaacgaac agcccgcccg tgtcttcgat ggtcagaaca cgtccgcgat acagcccttc
     9541 gaccaatact tttaccgtgc cgtcgggcag tttcaacact tgcaggactt gtgcgaccgt
     9601 accggtctga tacaggtcgg cggcaaccgg ttcttctacc gccgcatcgg tttgcgccaa
     9661 caggaaaacc ggctcctcgc gggtaatggc gttttccagt gcggcgatgg atttcggtct
     9721 gccgacaaac agcggcagaa ccatatgcgg gtaaacgacg acatcccgca aaggaagggt
     9781 tgccaaggcg gcatattcct caaaatgctt ttctttttgt gtcataggta ctctcttgtg
     9841 tctgacagat tgccgatttt cgcgtacatt ggggttgaag gtattatttc aagcatatgt
     9901 ggtttattta tggagtttga tgcgatgccg tctgaaacat tctggcttca gacggcatgg
     9961 gcttggaaag acaaggcagg aacaaaaaac tgttctgtgt tgccgctcct tgctgtacca
    10021 tccgtatggt ttacggttct gccgccctat tttcaaaaca tgacgcaggt atcccatgtc
    10081 ttcttttatt tttcccgata acgggttcga atggcgcaac gaaagtctgc aaaagccgcc
    10141 caaaggttgg cattatgtcc gcgaaagcgg tgcagacggc attttgaagg ctgcctatca
    10201 aaatattgca actgtctggc agggcgattt ccacaatgcc aaacaggtgc tttctgcaat
    10261 gaagaagagg gttcgcaaac ctgccgccgt ccgttccgat gcggatatcg ccgccctttt
    10321 ccatgcccac cgtatgaagc aggcacagca gagccgtatt ctgaatatgc ttgccgttga
    10381 aatccgcccc ggttttgtgt tggacaacaa acgcgcgccc gatatacgct ccgctttgct
    10441 cgacgtgtac ggagaggcgg acggcaaacc gtttttccta ccgctcaatc tgctgctggg
    10501 gtttatgggt gcgcacgagt ggcataagaa aggggttgcc gttccgcagc tgggcggcag
    10561 catacacgtt cctttcggcg tattttcgcc cttgcgcggc gaatatctcg atttgctcgc
    10621 ccaagcgccg tcaacagggt ttcagacggc attggacatc gggacgggtt cgggcgtgct
    10681 tgccgcgata ttggcaaaac aggggattcc ttccgtaata gggacggaca ccaatccgag
    10741 ggcggttgcc tgcgcccggg ccaatatcgc ccggctgggt ttggaaaaac aggttgaaat
    10801 acgggaaacc gatatgttcc ccgatatgcg tgccgatttg attgtctgca acccgccgtg
    10861 gctgcccgcc aagccgactt ccgccgtcga atccgcatta tatgaccccg attctgcgat
    10921 gttggccgcg tttttaaggg atgcgccgaa acatctgaat cccgacggcg aaatccgcct
    10981 gatcatttcc gaccttgctg aacatctgca cctgcgtccg tccgattttc tggataaggc
    11041 atttgctcag gcgggtttgc gcgttgcgga tgttttgaaa accaaaccga ttcacaaaaa
    11101 agctgccgat ccgaacaatc cgctggcttt tgcccgaaac agggaaacta ctttcctgta
    11161 ccgtttgaaa aaggcataag gggcggcggt gcgcttccgg cggcaggcat cggaacgccc
    11221 gcgccgccgg cacgacatca ttcgggcgga ttattcttgt gaaaataccc gctcaagcat
    11281 actgcccaat gccgtctgac gcgttttgac ggtggcggca gcttgccgga aggcttcttc
    11341 cccgccgaag aatacgaact tctccctcgc gcgggtaatg gcggtatata acagctcctt
    11401 actcaatccg gacaatgcat cgtccccttc gtccgaaggt gcggcggaag gcggcagcag
    11461 ccagacttcc cgatattccg aaccttggct tttgtggacg gtcatggcga atgcgggttc
    11521 aaattcgggc aggcagctta ccgctacctt tttaaatccg tccgcatcgg caaaataggc
    11581 ggcaaggctg ccctgccgtc cgacatcttc cataatcagt ccgatgtcgc cgttgaacag
    11641 ttcaagcgcg tagtcgttct gtctgatcat aatcggttct ccggcgaaat aggcctgatg
    11701 ttccggtatg ttcattttgc ggcgtacatg gcggcaatag gcttcgttga agtcttccga
    11761 atcctgccgc caagctgcca gaaccacgat atccgagatg cccacgtatg cggcttcgat
    11821 attgccgtct tttaccgcct gccaataggc tttgtgtgcc cggtacaacc tttcgacttg
    11881 agcgttcgga ctgcattccg aatgttccag ttcgtccgga aaccggtcaa acaatgccca
    11941 cgccccctca tcgcccgata cggcggcacg ggcaaggcag ccgatgccgc tgttgtcgcc
    12001 gaagcggtgg ctgaacgaca gatgggcggt gttttgcgcc agcacgggag catcttcgtg
    12061 taccggaaat ccgtgttcgg gcaggtagag gttcaaactt ttatacgttt ctctgtccaa
    12121 aatggttttt tgcgacaaaa cggacagcac cgccccgatt ccgacggacg ggagctggtt
    12181 ttcatccccc agcagaatca cgcgcgcgcc ggttttgacc gctttcaaaa gttgcagcat
    12241 caatgcggta tccaacatgg aggcttcatc gacaatcagc acatcgaacg gcagcggacg
    12301 gatatggtcg aacgccgcct gcattttggg cgggcgcagc ttcagcaatc ggtggacggt
    12361 ttgcccttcc agtttgagca aatggcggcg gacggcctcc ggcgcgtcaa aaccgttgat
    12421 tgcacggtgc agcgcgcgcg ccatatgtgc cgccgctttg cctgtcggtg cggcaagcgc
    12481 gatgttggga agattttcgt cttcaccgca aatcagcgcc agcagtttgg caaccgttgt
    12541 cgttttgccc gttcctggcc cgccggtaat caccataaaa gactgcaaca gtgccaaggc
    12601 ggcggcatcg cgctgccctt cgctgcccgt gccttgaaac cattttgcga ggttttgccg
    12661 cgcgcctgcc gcgtcggggg cggacgtgcc ggctgccgcc aagcgtttta tctcggcagc
    12721 caaatcgtat tccaactgcc acatcctgcc caaaaacagc cttctgcctt ccaaaatcaa
    12781 aggcgcggcg gatgttccga ccacgggtgc gagtgccgac agcgcgtcag cctcgccacc
    12841 gctcaaacgg ataaacgaat gaccgttttg caatgcctga aacaggcgtt cggtgcagtt
    12901 tgcaagcact tcgtcgccgg aacccgcata gtgttccaaa aaacggattg ccgcccttgc
    12961 cgccgcttgg gcaaattcat ctgtctgccg ttccattgta ttccttgtcg aaatgccgtc
    13021 tgaaggcgcg aggcttcaga cggcgcgtgt tgtttcggct gtttaggcat ttgcgccctg
    13081 tttggggtgc aggctgccgt ctttcatgac catcacgcgc ccgaagcagc cggcgagttc
    13141 gtcgtcgtgc gttacgacca ccagccccgt tcccaattct gttttcagtt ccagcatcat
    13201 atccaaaaca ttcctggcgt tcgcacggtc gaggttgccg gtcggttcgt cggcaagcag
    13261 gcattttggt tgcgtaacca aggcgcgcgc aatggcggca cgctggcgtt cgccgccgga
    13321 cagttcgccc gcgcggtgcc tcgaacggtg tttcagtccg accttttcca gcatcgccat
    13381 tgccgtttct gctgcctctt cacggctttt tttgccgatc agaagcggca tcatcacatt
    13441 ttccagtgcc gaaaattcag gcagaagatg gtggaactgg tacacgaaac cgagatggcg
    13501 gttgcgcagg gtgcccagtt gccgctggct taatgtacgc aaatcctcgc ccatcagcag
    13561 caccctgcct tcagacggca tatccagccc gcccaaaata tgcagcagcg tcgatttgcc
    13621 gctgcccgaa gaaccgatga tgccggtact ttcccccgcg aggatttcca aatccaagcc
    13681 gtgcagcacc cgaacgtcca aaccgccgtc acggtagcgt ttgcccacgc cttcgcattt
    13741 caaaatcaac tcactcataa cgcaaagcct ccgccggttg ggtttttgac gcgcgccagc
    13801 tcgggtagag cgtcgccacg aaagacagtc ccaaagaaat gcaggcaatc agggcaacgt
    13861 cgcccatatc gacatcgctg ggcaggtagt cgataaaata aacctgcgaa ttgatgaggt
    13921 ggacaccgag caggttttca aaaaacgcca cgaccctgcc gacgttccaa cccaaaagca
    13981 cgccgcagac cacacccgcc agcgtgccga aaaagcctga aaacgcgccc tgcaccataa
    14041 aaatcttcat cacgccgcca ggggaaagac ccaaagtccg taaaatcgca atgtccgcct
    14101 gcttttccgt aaccgccatc accaaggaag agacaaggtt gaacgccgcc acggcgataa
    14161 tcagcgtcag gatgatgaac atcatccgtt tttccaactc gaccgcctca aaatagctgc
    14221 ggttgttgta cgtccaatcg cgcacccaaa ccgcgtccct ttgcgcctcc ggaatcagtg
    14281 ttgccgtcaa ggcgggggcg ttttgcgggt cggcgagttt cagccgcagc cccgcaactt
    14341 ccttatccaa gcggtacagt acgcgcgcgt cttggatatg cgtcatcgcc aatgagttgt
    14401 ccacttcgta aacgcccgtc ttaaccagac cgaccacggt aaactgcttc aggcgcggca
    14461 cgactccggc aggggtaacg ttgccctccg gcgtgatgac ggtaacttta ttgccgactt
    14521 ccgcccccaa agcctccgcc aagccgacac cgaggataat gtcaaactcg cccggaatca
    14581 ggtcttcaaa tttgcctgcc ggcattttgt cgccgtattc caccactttg cgctcttcag
    14641 acggcaaaat gccgcgcatc tgcacgcccc tgatttcgcc cgcattggcc agcaatgcct
    14701 gattggaaac atagggcgcg gcagccaaaa tacctttgcg gttttcggta aaccgaagca
    14761 ggttgcgcca atccgtatcc gtattgtcga tatagccgat ttcggcgtgc ggcgcgacat
    14821 tcaggagctg cccgcgtatt tctttctgaa agccgttcat aaccgacaag acgacaatca
    14881 gcgcggttac gcccaaggcg attccggcaa tcgaaaccat cgtgataaac gacataaagc
    14941 cgttgcgctt tttcgccctg agatacctca agcctatcca agcctctaga gaaaacataa
    15001 cgctacctta aaaatgtctg caaacgtgcc gccccggacg gcggtttggg ggcggcggca
    15061 aaagtttatt gtaccgtaaa accccggcag cgtccgaacg cccgagtgcg ggggacgggg
    15121 cggcagagcg ggcggaaaac ttgcacaacg cgtcaaactg ccctatcctt tccttcagaa
    15181 aaaccgtttc ttgaggaaaa caatgaatat ccgaactgct tttgctttgt gcgccatcgc
    15241 cttatccgcc gctgcggctg cctacgccaa agaaatcaaa atcgatgcca acaacacgcc
    15301 ttattccgaa gccgacgcgc aaaagctggc ggcaacggca gtcggtatgg gtgttaagga
    15361 acctatcagc ctgaacggcg gcagcggcag cattaccgtg tccggcagca gcgcgacgca
    15421 gtgcgtgttc aaagtcggca acggaggcgc gttgcagatt caagggctaa actgcaagta
    15481 aaccgcccgg aaaaatgccg tctgaaggct tcagacggca tttttcattg gcggcgttat
    15541 gccccgcctt ctttaatcag gcggcgttcg tacaccgcct gcgccagcgt tcccgcatcg
    15601 acatattcca attcgccgcc caagggaatg ccctgcgaca gcctgctgac tttgtaaggc
    15661 aggtttttaa aaaactcgga caggacatac gccgtcgcat tgccttctgc ggtaaaagcg
    15721 gttgcaataa tgatttcttc gacttccccg ccgcccagcc gttgcgccag cctgtccaat
    15781 gcgatggcgg atacgtccat tcccaatgcc gtattgattt gccccatcag gacgaaatac
    15841 agcccgtcgt ggcagtttgc cgcttccata ttcgacacgt cggcaggcat atgcaccacc
    15901 atcagccgcc gcccgtcgcg tgtttcatcg gcacaaatat cgcacaatcc gccttcgcaa
    15961 aacgtgttgc acatcgcgca atggtaaacc tgcttcaatg ccgtctgcaa ggcatccacc
    16021 agtttttcag cctctttgcg cttgtgttgg agcaaatgat acgctatccg ctgtgccgat
    16081 ttcggcccga cgttgggtaa aaccttcagc gcgtcgatca atccttggaa ggcatcttgt
    16141 tttttgtggc tcatcatatt ccgccgtatg ggaaaacggc cggaatattc cgaccgttat
    16201 tttgtcaaca aaagtgtcaa ttactgaccg tcgccgttgt cgaccgattg cgctcctttg
    16261 gtctgtttga ttttgccgtt gaaataacgt atcaacaagt cgaaagtatt ggcagactgc
    16321 tgttgcgcca aagcctgttt tgcaagcgga agctgtgcgg cgatatcatc cggcggggtt
    16381 acagcctgta cttcgacaat cacgggtgcc ggcagaccga tcagcctgac gtaggcgggt
    16441 ttgccgtttg ccggttttgc tttcagcagt tccgcataag cctcgggcgg catggactgc
    16501 cttgcctgct gcgcgcccaa aacggacact tccgaccatt tcacgtcaac agccttgccg
    16561 ccgttcagtt gggtaagcac gtcttttgcc ttgttttcgg caagtttggc ggcttcggta
    16621 cggatataag cttgacgtac cgcgtctttg gcttcggcaa acggcagggt tttctcttcg
    16681 cggacttctt tggcgcggac gacccacgcg gtttcgctgt tgatggtcag cacttcggaa
    16741 ttgtgttttt tcttcaatac gtcgtcgctg aatacggcat tgatcaggtt ttcgggcata
    16801 ccagacattt gcgcatcctg cctgctcagc caagtttctt gggtttcgac tttcaaaccg
    16861 ctgtttttgg cggcttcggc aagcgaggaa ggatggttga acgcgtcatc gcccagcttt
    16921 tcttttgcct tattgaagtc ggcaaccgcc tttttcattt tcaattcgtt ttcgacggcg
    16981 gctttttcct gctcgaaaga aggtttggct tcatttgtcg gcaaacgcgc cacgcgctct
    17041 tcaaacgcat ttttcacttc cgtttcactg acggtctgtt tgtctgcaaa atccttcaga
    17101 ttcaaggcga catattctaa tttgaccgcc tgcggcagca gatagtcttt tttgtttgcg
    17161 ttataaaact tctgcaaatc ggcttcagac actttgactt gggcgatgaa ttcgtcgggg
    17221 ttgaaagtgt gcgaacggat ggtgcggttg acctgcgtca gcctgatcag ctgttccgcc
    17281 tgcgcgtcgc cgaccaatac gccgttttgg acgaggttta ccaaattctg caaggcaaac
    17341 tgatcgcgga tttcttcgac aaactggtct tcagacatat ggcgttggga aaggtagcgg
    17401 tttaaaagcg cgtggtcgaa tttgccgttt gcgtcgtgga aattgggatc gtccacgata
    17461 atttgcttga tttgttcgga agaaaccgaa atgcccatca gcttcgcgcc ctgtttcagg
    17521 taggcgcgtt gcagcaggga ttggaacacc gcgtcgcgcg aagggccgcc gccgcccgcc
    17581 tgttcgtttt ggacggcggc gttaagcgac tgctcgctga ttttttcgtc gcccacttgg
    17641 acgatgtagt cgacacccgg atgggatacc gtgctgaccc cgaagccgac gaaggtcaat
    17701 gcaatcaggc ctaataagac ttgggcgggc gttctgtatt tttcgatgga atggaacata
    17761 ttttaaatcg ggatatagaa tgggaacggg aaattcaagt cgggtattgt aacggttttt
    17821 atccctgtct gcacggggct tgccggttga agatgccgtc gtaggtttct tcgctgaagc
    17881 cggcgtaaac cttgccgccg cactccaata cgggacgctt gatcaggctc ggcatttcgg
    17941 acatcagttt gacggcctcc actgtcgaga acagggcttt ttgctgtgtt tcggcatcga
    18001 gtttgcgcca gcttgtcccg cgtttgttga gcagggttgc caaaggcact tgttccagcc
    18061 acgagcagat ttccgcttcg gacggcctct gttttttaaa atcccgaaat tcaaactcca
    18121 agccgtatcc ggcaagcctg tttttggctt ttttgactgt gtcgcaattt ggaatgccgt
    18181 gaaggattat catttggaaa ccttttgtct gaaataataa aacggatatt ttactataag
    18241 tgtctgaaaa tttgcccgtc tgtttcagac ggcggggcgg ttatgttaca atccgaaaat
    18301 tcgaaaaatt taatctcttg ttcaataaag gctttaccaa tcatgatttc taccaacggc
    18361 atcaccatgc agttcggcgc gaagccgctg tttgaaaatg tatccgttaa attcggcgaa
    18421 ggcaaccgct acggtttgat cggcgcgaac ggctcaggca aatccacctt catgaaaatc
    18481 ctcggcggcg atttggaaca gacgaccggc gaagtggcga ttgaaaacgg cgtgcgtttg
    18541 ggtaaattgc gccaagacca gtttgcctac gaagacatgc gcgtgctgga cgtggtgatg
    18601 atggggcata ccgaaatgtg ggcggcgatg accgagcgtg atgcgattta cgccaatccc
    18661 gaagccaccg aagacgacta catgaaagcc gccgaactgg aagccaagtt cgccgaatac
    18721 gacggctaca ccgccgaagc gcgcgccgcc gagttgttga gcggcgtagg catttccgaa
    18781 gatttgcaca atgcgaccat ggcagaagtc gccccgggct tcaaactgcg cgtattgctg
    18841 gcgcaagccc tgttctccaa accggatgta ttgctcttgg acgaaccgac caacaacttg
    18901 gacatcaata ccatccgctg gttggaaggc gtgttgaacc aatacgactc cacgatgatt
    18961 atcatctcgc acgaccgcca cttcttgaac gaagtctgca cgcatatggc agatttggat
    19021 tacaacacca tcaccatcta tccgggcaac tacgacgact atatgctcgc ctccgcccaa
    19081 tcgcgcgaac gcgccctgaa agacaatgcc aaggcgaagg aaaaattgca ggaattgcaa
    19141 gaatttgtgg cgcggttcag tgccaacaaa tccaaagccc gtcaggcaac cagccgtctg
    19201 aaacaggcag acaaaatcaa atcggagatg gtcgaggtca aaccctctac ccgtcaaaac
    19261 ccgtatatcc gttttgaagc cgatgaaaaa gccaagctgc accgtcaggc tgtggaagtt
    19321 gaaaaactgg cgaaacgctt tgaaacccag ttgtttaaaa acctgaactt catccttgaa
    19381 gcgggacaac gcctcgccat catcggcccg aacggcgcgg gcaaatccac cctgctgaaa
    19441 ctcttggccg gcgcgtacaa ccccgaatat tcagacggtc tgctgccgga cgaaggcagc
    19501 atcaaatggg cggaaaaagc cagcgtcggc tactatccgc aagatcacga aaacgacttc
    19561 gatgtcgata tggatttgag cgaatggatg cgccaatggg ggcaggacgg cgacgacgaa
    19621 caagtcatcc gcggcacttt ggggcgtttg ctcttcggca gtaacgatgt cgtgaaaaaa
    19681 gtgaaggttc tctccggtgg tgaaaaaggc cgtatgcttt acggcaaact gttgctgttg
    19741 aaacccaatg tcttagtcat ggatgaaccg accaaccaca tggacatgga aagcatcgaa
    19801 tccttgaaca tggcgctgga aaaatacaac ggcacgctga ttttcgtctc ccacgaccgt
    19861 cagttcgttt cttccctggc tacccaaatc attgaattgg acggcaaagg cggatatgaa
    19921 cactacttgg gcgattacga aagctatctc gagaaaaaag gcgtagcata accgccggtt
    19981 ggaacaatgc cgtctgaagc ggcttcagac ggcattgttg ataactttaa aataggaagc
    20041 atatgcagac ttatctcgtc ggcggtgccg tccgcgatta tcttttgggc ttgcccgtca
    20101 aagaccgcga ttgggtggtc gtcggcgcag acgcacaaac catgctggcg caaggcttcc
    20161 agccagtcgg caaagacttt cccgtgtttc tccatcccga aacacacgaa gaatacgccc
    20221 tcgcccgcac cgagcgcaaa accgccaaag gttacgtcgg tttcagtttc cacgccgaca
    20281 aagacgttac gctggagcag gatttgatgc gccgcgacct gaccatcaac gcgatggcgc
    20341 aagatgcgga cggcaagatt atcgaccctt tcggcggaca acgggatttg gcggcaggca
    20401 ttttgcgcca cgtttcccca gcctttgccg aagaccccgt ccgcatcctg cgtactgccc
    20461 gctttgccgc gcgttacaag tttgaaatcg ccgaagaaac cataaagctg atgcggcaga
    20521 tggtggaaaa cggcgaagcg gacgcattgg ttgccgaacg cgtctggcag gagtttgcga
    20581 aaggtttgat ggaaaaaaat ccgcgcaaaa tgattgaagt gttgcgcgaa tgcggcgcgc
    20641 tcaaagtctt gctgcccgaa gtcaatgccc tcttcggcgt gccgcaacgc gccgactacc
    20701 atcccgaaat cgacagcggc atccataccc tgatgacgct gcaacgcgcc gccgatatgg
    20761 gtttgagcct gcccgaacgc tatgccgccc tgctgcacga cttgggcaaa gccaaaacac
    20821 cgtccgacat cctgccgcgc caccacggac acgacctcgc cggtgtcgaa cccgtgcgca
    20881 aagtcaatca aaggctgcgt gcgccgaaac attgtgccga gcttgccgaa ttggtttgcc
    20941 gttggcacat tattttccac caagtcggac agcttaaaag ccaaaccatt ctgaacgtat
    21001 tgaaaaaaac cgacgctttc agacgacccg aacgctttca gacggcattg aacgtctgca
    21061 ttgccgacac gcaaggccgt ctgaaccgcg aacacacgcc ctacccgcaa cgcgcgcact
    21121 ggctcgccct gctcgaagcc gccaatcagg tagattcggg caaaatcgcc gccgaatgct
    21181 gggcgcaggg aaaagcgcac cttatcgccg aacaaatcga ccgggcgcgg ctggcgcaaa
    21241 tcgccccgct gcaaaaagcg tttcgagcgg cgcaagacaa aacagaaaaa cattaaaacg
    21301 cccaatgcag ccactt
//
LOCUS       DAXHSJ010000019        20690 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000019 DAXHSJ010000000
VERSION     DAXHSJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20690
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.019"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(63..239)
                     /locus_tag="ACFM8U_000517"
     CDS             complement(63..239)
                     /locus_tag="ACFM8U_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333806.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(242..1600)
                     /gene="murF"
                     /locus_tag="ACFM8U_000518"
     CDS             complement(242..1600)
                     /gene="murF"
                     /locus_tag="ACFM8U_000518"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC6333807.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALANNALRAHVGCGFDGVGDIAKAKSEIYAGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGVDSGDVHAENIVLKPLSCEFDLVCGNERAAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLDDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAIDVLVRMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEEFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1725..3374)
                     /locus_tag="ACFM8U_000519"
     CDS             complement(1725..3374)
                     /locus_tag="ACFM8U_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6333808.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVSGGGVFADVKNYKRRSKIWLT
                     ILLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAK
                     KASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTA
                     HATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYP
                     WFTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDAR
                     RFQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTL
                     THGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDF
                     WGNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3397..4875)
                     /locus_tag="ACFM8U_000520"
     CDS             complement(3397..4875)
                     /locus_tag="ACFM8U_000520"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC6333809.1"
                     /translation="MFSKLTPLAETGILPLPCANAAGRLLHSDSRQIKQGDIFVACQG
                     EYTDGRSYIPAAIANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGMLAAQVYGN
                     VSGGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRRQGATAAAMEVSSHGLDQSRVNGVPFCSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLQHAIINVDDEYGAELVGRLKKDCPDLAVYGYGFSEHADICITDF
                     TASSDGMEAVFQTPWGEGRCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLEKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRLLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4900..6645)
                     /locus_tag="ACFM8U_000521"
     CDS             complement(4900..6645)
                     /locus_tag="ACFM8U_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC6333810.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTVLTHDGELLPVSFE
                     KQAVAPKGKRVIKASTAKKVRELMVSVTEAGGTGTAGAVDGFDVGAKTGTARKLVNGR
                     YVDNKHVATFIGFAPAKNPRVIVAVTIDEPTANGYYGGVVTGPPFKKIMGGSLNILGV
                     SPTKPLTAAAVKTPS"
     gene            complement(6706..6969)
                     /gene="ftsL"
                     /locus_tag="ACFM8U_000522"
     CDS             complement(6706..6969)
                     /gene="ftsL"
                     /locus_tag="ACFM8U_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC6333811.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6966..7952)
                     /gene="rsmH"
                     /locus_tag="ACFM8U_000523"
     CDS             complement(6966..7952)
                     /gene="rsmH"
                     /locus_tag="ACFM8U_000523"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC6333812.1"
                     /translation="MSGAESYRHITVLLDEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGSIPEQSQRKTSEWQ"
     gene            complement(7949..8404)
                     /gene="mraZ"
                     /locus_tag="ACFM8U_000524"
     CDS             complement(7949..8404)
                     /gene="mraZ"
                     /locus_tag="ACFM8U_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC6333813.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            8710..9900
                     /locus_tag="ACFM8U_000525"
     CDS             8710..9900
                     /locus_tag="ACFM8U_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6333814.1"
                     /translation="MSGNPINLHKLTLIMPLPLPSPEARQSSLKLQTLIAEKIGKHGN
                     WIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLARQLQELLSQT
                     AGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQARAPEASQKVV
                     HLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTYSARPLHDLQ
                     LSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQYYHPQRN
                     QGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQSHFLLNL
                     GITELLAQVGKTDSPAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVDWAGFRFGDI
                     CHKL"
     gene            10032..10108
                     /locus_tag="ACFM8U_000526"
     tRNA            10032..10108
                     /locus_tag="ACFM8U_000526"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10066..10068,aa:Met,seq:cat)
     gene            10116..10191
                     /locus_tag="ACFM8U_000527"
     tRNA            10116..10191
                     /locus_tag="ACFM8U_000527"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10149..10151,aa:Ala,seq:ggc)
     gene            complement(10482..11303)
                     /locus_tag="ACFM8U_000528"
     CDS             complement(10482..11303)
                     /locus_tag="ACFM8U_000528"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC6333815.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11336..11980)
                     /locus_tag="ACFM8U_000529"
     CDS             complement(11336..11980)
                     /locus_tag="ACFM8U_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6333816.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEREDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11983..12828)
                     /gene="ftsN"
                     /locus_tag="ACFM8U_000530"
     CDS             complement(11983..12828)
                     /gene="ftsN"
                     /locus_tag="ACFM8U_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6333817.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKADEVEEK
                     ADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEKKASKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(12934..14430)
                     /locus_tag="ACFM8U_000531"
     CDS             complement(12934..14430)
                     /locus_tag="ACFM8U_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC6333818.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSHRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14444..14764)
                     /locus_tag="ACFM8U_000532"
     CDS             complement(14444..14764)
                     /locus_tag="ACFM8U_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC6333819.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15247..16773
                     /gene="putP"
                     /locus_tag="ACFM8U_000533"
     CDS             15247..16773
                     /gene="putP"
                     /locus_tag="ACFM8U_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC6333820.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            complement(16863..17030)
                     /locus_tag="ACFM8U_000534"
     CDS             complement(16863..17030)
                     /locus_tag="ACFM8U_000534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333821.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            17022..20627
                     /gene="putA"
                     /locus_tag="ACFM8U_000535"
     CDS             17022..20627
                     /gene="putA"
                     /locus_tag="ACFM8U_000535"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC6333822.1"
                     /translation="MFHFTFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFLEWSATPTAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSTLAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVAHLINKALAKRNDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSKKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDNVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGVQAVVEPDSPWASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4865 a         5996 c         5035 g         4794 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagggacttt ctcaatcacg caaatcgggt aactccccgg atttttgccg
      121 ccacccaagt ctaaacataa atcttcataa agatacgcct gcgtctgcat tccagcataa
      181 aacgcgccga ccagcgcggc aatcaacaat aaggctttaa aaaaacgtcg acttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt cttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gggcgtacgc gccgacttcg gcgtgcatgg
      481 cggcggcttc gtcctcgccc agttcgccca tatcgcccat cacgaaaata cgcggcgcag
      541 gcatacgcac caacacgtca atcgcggctt tcatactgtc ggggttcgca ttataagtat
      601 cgtcaatcag ggtcgcgccc ttgatgccgg ctttgacgtt cagacggcct ttgatattgc
      661 tgaagccttg caaaccttcc gccacatcgt ccaaactcaa acctgccgcc aaagccagcg
      721 cggcggcggc ggcggcgttg tggacattat ggcggccggg aacaggcagc accacggcgg
      781 cgcgttcgtt tccgcacacc aaatcaaatt cgcacgacaa cggtttcagc acgatatttt
      841 ccgcgtggac atcgccgcta tcgacgccga aagtgcgcgt attcaaatta agcgttgccg
      901 ttttgaagac agccatattg gcatcttctt gaggaatcag cgccatgccg tctgaacata
      961 agcctgcata aatctcgctt ttcgctttgg caatatcgcc cactccgtcg aaaccacagc
     1021 cgacatgggc gcgcagggca ttgttggcca atgcggcatc aggtttggca atttgcgtca
     1081 agaccgccag ttcgccaaaa tggttcatgc ccatttcaat cacggcatag cggtgttttt
     1141 cgtttaattt caataaagtc agcggcaatc caatatggtt gttgaagttg cctgccgtcg
     1201 ccaaaacggc attatcgccg aaacggcggc gcaataccgc agccaacatt tccttcaccg
     1261 tcgtcttgcc gcccgagccg gtaataccga acacaaacgg gttcacattc tcgcgccacg
     1321 ccttcgccaa catttgcaac gcggtaagcg tgtcatcgac tttcaacgcg ccatccattg
     1381 caacgcaatc ttcgcgcgaa accacaaccg ccgccgcacc agcagccaat acgtcttcaa
     1441 caaaatcatg cgcgtcaaac cgcccgcccg ccaatgcgaa aaacacatcg cccgcgcgga
     1501 tgtcgcggct gtcggttacg atgcgcgaca cgggtttgct ttcagacggc atcggaagct
     1561 tgagggcttg gcagatgaaa tttagatcca gtggtttcat atttactttc gttaatattc
     1621 gggcggcgga cacatcggta gcggctgatt tttttatcgc ctgttttgct gtggtaaaac
     1681 acagattatt ttcccattct cattcggcat tttttctgta cgtatcattt tttagacgta
     1741 tttttagtcg atttgccttt tcccgcatac cacggcgcgg ggtcgtcggg cagtccgtcg
     1801 ataaaggcaa ggttattgcc ttcgccctgc acatcgggaa cattccccca aaaatcatag
     1861 ccgtcatcgg gcaactcgtc ggtttcgata cccgtccaac tgccgaatcc gcgtaaaaaa
     1921 ttaaacgcgc tgcgcctcac tttaatcatt tcgcgccgcg tgtcatcgga cgaaatttta
     1981 accagcggca cgccgtagct ttgacgcttc cacgcaccat gtgtcaacgt tcgctcgccg
     2041 cctttaccga catgcatcaa accgtggtcg gaaaagtaaa ccaaagacca gctttcttta
     2101 ttttcattca atatcttaac tgtatcttct aaaaatttat cggtttgcgc gatggtggaa
     2161 acatagcagg atattttttc agtttgatac tgaaaccgcc gcgcatcctt atccaaacgt
     2221 gtgcaaaaat cactgtgcga acccatcagg tgcatcacaa tcagccgagg cttcgtgcct
     2281 gcatttccta tcaaaacccg tttgaacgcc ggcaacaaaa ggcggtcgct caaccccgcg
     2341 cttttgccat aatcacccct ttgggtaaac cacggataat cgctgcgtag ggcataggtg
     2401 gaaatttcgt tggcaaaatg ccccaacatt ccttgattag acagccacgc cgtccgaaaa
     2461 cccgcctgct tcgccaagct gacgatgtta ttgttcggtt ctcccggcag ccccaaagtc
     2521 tgcggcagcg aaagattcgt cgcgtgggcg gtcgattggt aaccgtttat caacagccct
     2581 ttggtctgac tcaaaaaagg cgtatcgggc aatgggaaac cgtaaacatt catataatcc
     2641 gaacgcgcgc tctcaccgat aaccacaaca taatttttat acttgggcgc aacatgacgg
     2701 atatgccatg ttgacgcttt ttttgctgct tccaaaatgt gggcacgctt ggcggcatat
     2761 tgtgccggcg cggaagccaa atcgtaatac aggtcgaaaa tattcaacaa caggccggca
     2821 tcaggttctc gcaaatcttt atcgctggcg attttatcca tcaccgcgca ggacaaaatc
     2881 aaagtcaata atatagtcag ccatattttg ctgcggcgtt tatagttttt tacgtcagca
     2941 aatacccccc cccccgatac acaatatctc caaactgtca agccaaaaat gaaaaataat
     3001 gcctgcacaa aataaagcga cccgggaaga ttgccgacaa attcacgcgc ctcggcagga
     3061 ttgctttcca atatcgaacc gactatctga taagacggcg caccatacag ccagccgacc
     3121 ggcaaatata gggcggttgt gccgacgtaa atcagtaaaa caactgaaga aacgcgcggg
     3181 aaaccgcgtg ccaataataa taaaaataca accgaagcca aagcacccac ggcagaataa
     3241 cggtagccgt attcatattc caagtgataa cctgatgcga tggccgcgcc aaaacaaaag
     3301 gctaaaagcg cgcaaatgcc caatgtattt tttaatgtct gatccatatt ttttattcgg
     3361 gcggattgtt tcatacgatg ccgtccgaat atcccattat ttacgagtta acaaagcctg
     3421 tccgacgatt tcaagatcgg aaaaacggtg cttcacgcct tgtacatcct gatagttttc
     3481 atgccctttg ccggcaatca ggatgatgtc gtttgcggcg gcttgttcaa ccgcataacg
     3541 gatggcggcg gcacggtcgg cttcgacgca ttcgggcgcg ggaacggcag gcaggatgtc
     3601 gttgatgatg tcgtgcggat tttccaaacg cggattgtcg ctggtgacga cgactttatc
     3661 cgcgccctgt acggctgccg cgcccatcag caggcgtttg ccgcgatcgc ggttgccgcc
     3721 gcaaccgaat acgcaccaca aagccgcacc ctgcggtttg atttcctgca aggtggcgag
     3781 tgctttttcc aatgcgtcgg gcgtatgggc ataatcgaca acgaccaaag gtttgccgct
     3841 gttcatgatg cagtccatgc ggcctgaagc aggacggatt ttttccagta catccaatac
     3901 cttatccagc ggatagccgt tggcacacag caaggcgatg caggcggcga ggttttgcgc
     3961 gttgaaccgt ccgagcaggc gcgtgcggca gcggccttcg ccccacgggg tttggaatac
     4021 ggcttccatg ccgtctgaag aagcggtgaa gtcagtaatg cagatgtcgg cgtgttcgct
     4081 gaagccgtag ccgtaaacag ccaaatcggg gcagtctttt ttcagacgac ctacgagttc
     4141 tgcgccgtat tcgtcatcca cgttgataat ggcgtgttgc aggccgtgcc agtaaaacag
     4201 gcgcgacttg atagcgccgt aggcttccat cgtgccgtgg tagtcaaggt ggtcgcgtgt
     4261 gagattggta aagattgcgc tgcaaaacgg cacaccgttg acgcgcgact ggtcaagccc
     4321 gtggctggag acttccatcg cggcggctgt tgcaccttgc cggcggaaac ggtagagcag
     4381 ggtttggaca tcgacggggg cgggggtggt gtgcgtggtt tcttccaacg cgccccaaaa
     4441 gccgttgccg accgtgccga taatggcggt tttttcgccc aacaaatcgg cggcctgcgc
     4501 cagccattgc gtaatggagg ttttgccgtt ggtaccggtt acgccccaga ctttgaggcc
     4561 gcctgaaacg ttgccgtaaa cttgcgccgc caacataccg gcacggtgtt tcaaatcttt
     4621 aataccttga ttggggactt tccattcggg attccacgca aatttgccgt cgtcgtccca
     4681 aaaaacaaaa gccgcgccgt tggcaatggc tgcggggata taactgcggc cgtcggtata
     4741 ttcgccctga caggcaacga aaatatcgcc ttgtttgatt tggcggctgt ctgaatgcaa
     4801 caaacgccct gccgcgtttg cacacggcag gggcagaatg ccggtttcag ccaaaggggt
     4861 taacttgctg aacataaaac aatctcgttg atacttggat taagacggtg ttttgacggc
     4921 tgcggcggtc aggggcttgg tcggagaaac gcccaagatg ttcaggctgc cacccataat
     4981 ttttttgaag ggcggccctg tcactacgcc gccgtagtaa ccgtttgcag tcggttcgtc
     5041 gatggttacc gccacaatca cacggggatt tttggccggg gcaaaaccga tgaaagtggc
     5101 aacgtgtttg ttatcgacgt atcgtccgtt aaccaactta cgcgccgtac cggtttttgc
     5161 gccgacatcg aaaccatcca cagcaccggc agtaccggta ccgccggctt cagtaacgga
     5221 aaccatcaac tcgcgcactt ttttcgcagt agaggctttg atgacgcgct tgcctttagg
     5281 ggcaactgct tgtttttcaa agctgaccgg caacaattcg ccgtcatggg tcaagacagt
     5341 ataggcacgc gccaattgca acaggctcaa ttgcaggccg taaccgaaag acatcgtcgc
     5401 ctgttcgata ggccgccacc tgcgccaatt tctcaacaaa cctgcagttt cgcccggaaa
     5461 gcccgagtgc atacgcacac cgatgcccaa ctcatgatag aagtcataca tttcttcggc
     5521 accgaaacgc gcagacagtt tgcttgtgcc gacgttggac gatttctgca tgatgccgcg
     5581 cacatccaaa gaggggtaaa catgagtatc gcgcacggga gacggtccga ttttataagg
     5641 ctgcgtattc agccgttcgt tcaaatcggt tttgcccgca tccaatgcct tcgcaatcac
     5701 aaacggtttg attgccgaac cgggttcgat catatcggtt acggcacggt tgcgccgctg
     5761 ttcgctgtct gcccggccgg gcctgttggg atcgtaggcg ggcgtattgg ccaaggcgag
     5821 gatttccccc gtgcgggcat ccaaaaccac caccgttccg gcttttgcct gatggtattc
     5881 gaccgccttg ttcaactctt cataggccaa ggtctgaatc ctctgatcga gggaaaggat
     5941 gatgtctttg ccgtttttcg gggctttatt gcgcggggag tccaagctgt ccacaatatt
     6001 gccctgccgg tcccgcaaaa cgacttccgc gccgtcttcg ccatgcaggc tgtcttcaag
     6061 cgaaagttcc aaaccttcct gacctttgcc gtcaatatcg gtaaatccga tgacgtgtgc
     6121 aaacaggttg cccatcgggt aatggcgttt taattctttt tcaaatacaa agttttccaa
     6181 acccaaggct ttgacctctt cggcaacctt gggatcgagc tgccgcttaa tccagataaa
     6241 cgacttgcct ttctgttcga gcttgttcct caaaacatca accggcacat cgacaagctc
     6301 ggacaggcgt tccaattgtg cggcagacgg catttccttc atctctttag gcacggcaaa
     6361 cagggactcc gtcggcgcgc tcaacgccaa aaccgcaccg ttccggtccg aaaccgtacc
     6421 gcgtgtagcc ggcaatgttt gagtccgcac aatccggttg tcgccctgtt ctttcaaaaa
     6481 gttatacgtt accgtctgca gatacagtcc gcgagcaatc agaccggcaa acaagaccgc
     6541 tattgccatc aggacgaagc tgatccgtcc gttactggtc atcggctttt tgacctgctc
     6601 ttctttgggc agcatccgag gcttatattc gctcttaatc aacattttta cttctcgtta
     6661 ttattatcct gacgcaggaa tccgattccg gcacacaggc tgcttctatc tttgatgcgc
     6721 caccataaag gtattgcccg aaaccggcgg atggaggttt tgtttttctg ccgccgccct
     6781 gatcgcttcg tggttcgcca aacgcgcctg ttgcagcctc atttgcgcat aatcctgctc
     6841 caaggcgatt tcctgttttt tcgccttgtc caaagctgtg aagttgagcc tgtactggtt
     6901 ttgctgcatc acaacggaaa aagcggaaac gcacaccgca agcagcagaa ggaaattcaa
     6961 tttgttcatt gccattcaga cgtttttctc tgtgattgtt ccggtatcga accggcagtc
     7021 cgctccgcca cacgcaaaac cgcacttctc gccctcggat tggcggcaat ttccgcctca
     7081 cccggcttta atgccctgcc cacgattttc aggggcagct cgggcaaatc cgcttccctg
     7141 accgccgccc aacgcggcag aggcgcgtgt tgcgaatatt ttttgacaaa ctgcttcaca
     7201 atgcggtctt ccaacgaatg gaaagcaatg accgccaaac gtccgccctc tttcagacga
     7261 cacatgacct gcggcaatac tgcccctact tcttcaagct cgcggttaat aaagatgcgg
     7321 accgcctgga aggtgcgcgt cgcaggatcc tgcccccgct cgcgagtacg gacgttttgt
     7381 gccacgatct gcgccagctt gcgggttgta tcgattggac tttccgcccg ttgcgcaaca
     7441 atggcgcgcg caatctggcg gctaaaccgc tcttcaccat aattcttgat tacctcgtgc
     7501 aaatcctgtt ccgacgcaac cgctatccac tctgcggcag acataccgcg cgtcgtatcc
     7561 atacgcatat ccaaaggggc atcgaaacgg aagctgaaac cgcggctgcc gtcatcgatt
     7621 tgcggggacg aaatccccaa atcaaacagc gcaccgtcca ccttgccgat acccaaaccg
     7681 tccaatgccg tctgaaacga agcaaaaccg ccatgcacga caccgacccg tttgtccgaa
     7741 cgcgccagct cttctgccac agcaatcgcc tgagggtctt tgtcgaaaac aatcaaccgc
     7801 cccgcatcgc ccaaacgcga caaaatcagc cgggaatgcc ctcccctgcc gaacgtaccg
     7861 tccacataga caccgtcttc gcgcacggca agcgcatcca ccgcctcatc cagcaagacc
     7921 gtgatatgcc ggtaactttc tgctccactc acaattgcaa atccgtctga ctcaactgga
     7981 aggcaagttc gtcaggatcg tcatccaaag cctgaaccat ttcggcctcc cactgctcac
     8041 gaccccaaag ctccaaacgg ttggcacgac cgaccaaaac gacttcacgg tcgaaatcca
     8101 cccttttcct cagtccggca ggaagcagca cccggccggc gctgtcccat tctaaaattt
     8161 ccgcgttatg cagcaaaaga ttttgaaacc gccgcaaaac agggttatcc gccactttta
     8221 agtttaaaag ttgcgccgca accttttccc actccgcaac agggtacatc aacagcttgt
     8281 gtttcgactc gagcgttacc actacggcag gcgtatagag gcgcgacaga atgtcacgga
     8341 atttggcagg aacagccaac cgccccttac tgtcgatgct taattcgtgt gcgccgccga
     8401 acatgacatg tcccaagccg aaatcaaaat cacaagggta aaagagacac tttgccccac
     8461 aattccccac caatcgacac tataagaaat tttaaacact cggtcaaatc agggcatgaa
     8521 aactcattaa catatctgaa atttttattc cttttaaaaa caataagata aaaaatgacg
     8581 acaacggcac ggcgggtgcg gtacagagtg accgaaccaa taaacaacta tatatgatta
     8641 atttaataat ataaacacaa tatatagtat taagataaag ccatgacagc acccgtacca
     8701 acgtgtaata tgtcgggaaa tccaataaat ttacacaagc taacacttat catgcccctc
     8761 cctctcccct ctcccgaagc acggcaatcc tcgctcaaac tgcaaaccct cattgccgaa
     8821 aaaatcggca aacacggcaa ctggattcca ttttcacgtt ttatggaatt ggttttatac
     8881 gctccgcaat acggctacta caccggcggc agccataaaa tcggcaatac cggggatttt
     8941 attactgcac cgaccctcac ctctctgttt gcacagacac tggcacgcca acttcaagaa
     9001 cttctatccc aaacggcggg caatatctat gaattcggcg cgggaaccgg acagcttgcc
     9061 gccgatttgt tgggcagcat ttcggacggc atcagccgtt actatattat tgaaatatcg
     9121 ccggagctgg cagcacgtca gaaaaacctg attcaagcac gcgcaccgga agcatctcaa
     9181 aaagttgtcc acttgaccgc acttcccgaa gcgtttgacg gcatcatcat cggcaacgaa
     9241 gtactcgatg ccatgcctgt cgaaatcgtc cgtaaaaatg aaggcggctc attcgagcat
     9301 gtcggcgttt gcctagataa tgaccgtttt acctattcgg cacgaccgct gcacgactta
     9361 cagctatctg ccttggcttc cctctatttt cctcaaacag attatcccta caccagcgaa
     9421 ctacatccgc aacaatatgc ctttatccgc acccttgcct caaggcttga acgcgggggt
     9481 atgatattca tcgactacgg ttttgatgca gcgcagtatt accaccctca acgcaatcaa
     9541 ggtactctga tcggacacta ccgacatcac gttatccaca atccttttga cttcatcggg
     9601 ttggccgacc tgaccgcgca tgtcaacttt accgacattg cccaagccgg tacggatgca
     9661 ggactggatt tgacaggcta tctgccccag tcccattttc tgctgaatct gggcataacc
     9721 gaactgttgg cacaagtggg aaaaacagac tcgcccgcct acatccgtga agctgctgcc
     9781 gttcagaaac tgattgacca gcatgaaatg ggcgaactgt ttaaagtcat cgcattcgga
     9841 aaaaatatcg gcgtcgactg ggcaggattc cgcttcggcg acatctgcca caaactctaa
     9901 ccctcatgcc gcctgaatcc gcttcagacg acataaactt tttaacattt aaaaacagtc
     9961 aactaattca aaattaaaaa atacggcttg tcaaaaaaac agaaaaacat ataatagcgt
    10021 cttcacgaaa cggcgaatta gctcagtcgg ttagagcaga ggaatcataa tccttgtgtc
    10081 cggggttcga gtccctgatt cgccaccaaa ttttcggggg tatagctcag ttggtagagc
    10141 gcttgcatgg catgcaagag gtcagcggtt cgatcccgct tacctccacc agataaaaaa
    10201 gcacagaccg taaaaaggta tgtgcttttt tattacctga ttaccggcaa ataaagaata
    10261 aaccactgcc ttcaaaacag gcaatcgact ttaaacctat cgccccgcct gtcctgattt
    10321 tatagtgaat taaatttaaa ccggtacagc gttggctcgc cttgccgtac tatctgtact
    10381 gtctgcggct tcgtcgcctt gtcctgattt ttgttaattc actatatcag cccgccagac
    10441 aaacccgacc cgaataatgt cttcaggtcg ggtttatggt ttcattccca acttatccag
    10501 cctgacagcc acaatataat gatggcaata ccaaaaacaa tgcggtaata ggcaaaagga
    10561 atataatttt tcttggaaac aaacctcagc aacgctttga ccgccaccaa gcctgaaaca
    10621 aaggcagcaa caaagccaat caaaatcaaa ccgacatcat gcagggtgaa aaaccggtaa
    10681 tgcttcagga catcataagc cgttgcggca accatcatcg gcacagccaa gaaaaacgag
    10741 aattctgtcg cagttttccg ttcgattccc caaagcatcc cgcccataat cgtactgccc
    10801 gaacgggacg tacccggaat cagtgcaaac acttgggcaa cgccgatcat caaggcatca
    10861 atcggacgca atgcatcaac atcggcaatt ttaggctctg ctcggctttg gcgtttctcc
    10921 acccacaaaa taaaaaaacc gcccaaaacc agcatgactg caacactcaa ggggttaaac
    10981 agatactctt tgatttgttt gccgaacaac agccccatca cggcggcagg tataaaagca
    11041 atggcaagat taaggacgaa acggttggct ttccggtctt ttcccacgcc atgcaacaca
    11101 ttgctgaaac gttgccggta ttcaaacact accgccaaaa ctgcaccgag ctggatggca
    11161 atttcaaaaa ccttgtgatt gctgtgaaaa ccaatcagat tgccgaacac aatcaaatgc
    11221 ccggtgctgg aaatcggtaa aaattcggta aaaccttcta ccaagcccat catcagggct
    11281 ttcaggacaa tcagaaaatc cattgcttgc gcttctttcg gatacgggag ttcggctatt
    11341 tctgtacagc aggggtctga cgcttgcgtt cttccctgac tttggcaacc aattctttaa
    11401 tcgtatgaac gccgccgtca aagccgttat tgaagataac gcggtatttt ccgccgacaa
    11461 taacggtcgg cgtgctgtca ataccgtatt gttccgtcag tttctgcatt tttaatgcgg
    11521 cggcggcagc ttcgggagaa tcataggcgc gcatcagttt tttgccgtca aagccttttt
    11581 gagacaaagc ccactttccg gcaaccgacc tgttttccaa gcggattttt tgttcgtaaa
    11641 ctgctttaaa cacagcaggg tttgcctgat atttcaaacc cgacaaattg acggcagccg
    11701 ccatcctggc cagaccgagc atttcaggct gccagaccac gtgctccgtc ctcaaatagg
    11761 catcagacgg caatgccttg cccaatttca ataacaaagg atcgaaatga tggcaatgta
    11821 cgcagaaata gccgaaaaat tccaaaacct caattttgcc cgactgttct tgaggaatgg
    11881 gtttatccaa cacaagatag tcttcccttt ccgtcagggc atatgcctgc gcggacaaca
    11941 ctgccgacag cagcagcggc aacagatgtt tgagcttcat aattatttgc tttcgataga
    12001 acggatcagg ctggcgactt catgtttttt caactcgtcc tgcatttttt tcaccgcatc
    12061 ggcagacata ttgccgcttt gcacccggta aagcgtttta tgtcccgcct gataaccgac
    12121 caccttggaa gatatgccca agattgccag tttggcacgc tgtccttccg cgctccggcg
    12181 gtcggcatac gcgcccattt gcagataatg cgttgcttcc gccttgtcgg gcgttttcat
    12241 tttctgcact tctttggcag cggcactgcg cgctttttcg atgctgccgc tgttgaggat
    12301 ttgttccggg gtcggtttgg gtgcaacttt ttccttctcc gcctttttct cttcttttga
    12361 agcttttttc tctgtttctt tagatggttt taccgcttgt tttttaaccg tttcggcatc
    12421 tttcttctgc gctttttccc cgacggtttg ttcacgctct tccgtcagtg ctttcttgcg
    12481 cactgcctgt ccgtccgact tttcccgctc cggctcgtcc gccttttcct caacctcgtc
    12541 ggctttatcg gcagcttttt ccgcatccga ctgctctgcc tctttcgcag catccggttc
    12601 ggacaaggcg ttttgatcgg ccggttcagg ttggatgtct tccttaggct ggtttttcgg
    12661 tttcaggatt tccgtttctg caggctgctt cgacggaacc gggattttga acgcattttg
    12721 accgctctgg ttcagataaa acaaaatacc ggcaataatg accgtcgcca gtatcaaacc
    12781 gaagaaaaaa ccggacagac cttttccgga ttgggaaaat ttgttcataa acatacctta
    12841 atgtgtttca gacggcatta acgccgtttg cttacgggcg gatattctaa caatatcgcc
    12901 atatttgggc aaaacctgct tccattcccg ttcctataaa gcgcgacgga aacctatggc
    12961 tttcatgacg tggcttctgc cgacttcttc gtcgcccgcc aaatccgcca atgtacgcgc
    13021 cacgcgcata atgcggtgga agctgcgggc ggaaagggag agtttttcca gcaggccgcc
    13081 caatgcttcc tgcgcttctt tttgaatgcg ggcttgggaa tcgagttcgc tgacgctcaa
    13141 agcggcgttg actttgccct gccgtgcgta ttgtttgtcg cgggcggcga taacgcgttc
    13201 caaaacggac gcgctgcttt cgcccgcttc ctgctgcatc agttcggcgg cggacaggct
    13261 ggggacttcg atggtcaaat cgatgcggtc gagcagcggg ccggaaatct tgctgcggta
    13321 acgcgcgacg ctttcgggcg tgcagcggca gggtttgacg ggatgcccga gataaccgca
    13381 cggacacggg ttcatggcgg cgacaagttg gaatttggca ggatagacgg cttggcgcgc
    13441 cgcgcgggaa atgtggattt cgccgttttc caacggttcg cgcaaaactt ccaaaacttt
    13501 gcggtcgaac tcgggcagct cgtccaaaaa caatacgccg tggtgcgcca atgaaatctc
    13561 gcccggacgc gggtcggaac cgccgccgac catagccgcc gcgctggcac tgtgatgcgg
    13621 actgcggaaa ggacggtggc tgtcgagttg ttgctggtgg ttgggcagga gcgaacgcaa
    13681 tgcccaaact tcaaccaatt cgtcttcggt cagcggcggc aggatgcctg gcagccgttg
    13741 ggagagcata gacttgcccg ttcccggcgg acccatcatc aagaggctgt gtccgcctgc
    13801 ggcagcgatt tccaaagcaa ggcgggcagt gtgctgacct ttcacatcac acaaatcagg
    13861 ttgtccgcca tgttcagacg gcatttgcgg tacggaacat tcggtttgcg ccaaaggttc
    13921 gataccgttc aaatgggcgg cgacttcgcc caaagagcgc gcgccgtaaa cggtaatgcc
    13981 gcgcatcacg gcggcttgtc ctgcgttttc ttcaggcaaa acaaatgcac gttttgcctg
    14041 cataccctgc cacgccatcg ccaacgcgcc gcgcacgggg cgcaacagcc ccgacagtgc
    14101 caattccccc gcaaactcgt attccgccag tttttcgggc gcaacctgcc ccgatgcggc
    14161 aaggatgccg attgcaatcg gcaaatcgaa acgccccgac tctttgggca ggtcggcggg
    14221 ggcgaggttg acggtgattt ttttggcggg gaattcaaaa ccgctttgaa taatggcggc
    14281 acggacgcgg tcgcgacttt ctttgacttc cgtatcgggc agtccgacga tgttgaaatg
    14341 tggcaggccg ttggcaaggt gggcttccac ttcgaccaac ggcgcattca taccgctcaa
    14401 ggcgcggctg taaaccaagg caagcgacat atttcagacg gccttattcg ccggcttcgg
    14461 tttgctgcct gatttcggcg acggcttctt cggcagcggc ttcagccgct tccaatgctg
    14521 cccgttcggg attttgcgcg gcttcgagtt ttgccaaacg cgcttccaaa gccgccagtt
    14581 tggtacgagt tttgattaaa acctgctgct ggatgtcgaa ttcttcgcgc gtaaccagat
    14641 ccatacggtt gaacgcgccg cccagcatcg ccttaatatt tttttccaca tctttggcag
    14701 ggctgttggc gatggtttcg ctgattttcg aaccgacttc ttcaaaaagc tgcttgccga
    14761 acataatctg tatccttcct gaacatatca aattcaatcg gctattgtat aaggaaaaat
    14821 gccgtctgaa aacgggcggc ggataatcgg caaaacatac cgcgcctcct ttgcggttgc
    14881 taaattacac ctcaaaacac ctgccgcctg aaaacggatt tcatattccg cgcaacgcca
    14941 gattacaaga cactacaaaa caatatactg ttttaaatga tttttccgac gcgcatcgtt
    15001 tcaaactcgg ctttttaagc cattaagtgc tttgcaaaca acagaaattg ggttatgctg
    15061 aaacggatta tttacaattt catatagttt tattacatat cttattgtga ttgaagataa
    15121 tttatccgaa ttcccctttc gggtatccgg attttccgtt gtacttttat tagaaaaaca
    15181 tttcaggcgc aagttgcttg caatttcaaa gcctgtaatt ctgaagtttt agacgaggaa
    15241 tagcacatga atcccatgta cattactttt gcaatctatc tggttgcagt ccttctcatc
    15301 gggcttgccg cctatttttc tacgcgcaat ttcgacgatt acattttggg cgggcgcagc
    15361 ctgggcccgt ttgttaccgc gatgtcggca ggcgcgtccg atatgtccgg ctggctgctg
    15421 atgggtctgc cgggcgcgat ttacctgagc ggtttgaatg aggcttggat tgccatcggc
    15481 ctcttggtcg gcgcgtattt caactggctc ttggtggcgg gccgcctgcg cgtgcatacc
    15541 gaatacgcca acaatgcgct gacgctgccg gattatttct tccaccgctt cggcgcgggc
    15601 ggacacttga tgaaagtggt ttccgcactg attatcctgt tcttcttcac gatttattgc
    15661 gcctcgggca ttgtggcggg cgcaaccctg ttccaaagcc tgtttgaagg tatgacttac
    15721 aatcaggcaa tgtggctggg cgcgggcgcg accatcgcct ataccttctt gggcggtttc
    15781 ttggcggtca gctggacgga tacgctgcag gcttctttga tgattttcgc cctgatttta
    15841 acgccggtga tggtctatct gggcttgggc ggcgcggaac agatgtctgc cgcgattcaa
    15901 agcgtcgccg caggcacggg caaagaatac ggcagcctgt ttgccggtac gaccgtcatc
    15961 ggcatcattt ccaccgccgc atggggcttg ggctatttcg gacagccgca cattttggcg
    16021 cgctttatgg cggccgaaag cgcgaaatcg ctggtatccg cacgccgcat cggcatgact
    16081 tggatggcgt tgtgccttgc gggcgcggtg gcggtcggtt atttcggcat tgcgtatttc
    16141 ggtgcaaatc ccgacaaagt ttcttctatg agcggcaacc acgaacgcat cttcatcgcg
    16201 ctttccaccc tgctgttcaa cccttggatc gccggcatca ttttgagcgc gattttggcg
    16261 gcggtgatgt ccaccctgtc ctgccagctt ttggtttgct ccagcgcgat taccgaagac
    16321 ttctacaaag gctttttgcg taaaaacgcg caacagtcgg aactggtatg ggtcggccgc
    16381 ctgatggtct tggcgattgc cgtgatttcc atcctgattg cttccgatcc gaacagcaaa
    16441 gtattgggac tggtgtctta tgcgtgggca ggtttcggtg cggcgttcgg cccgattgtg
    16501 attctgtccg tattgtggaa acgcatcact gcctacggcg cgttgtcggg tatggttgca
    16561 ggtgccttaa ccgtagtcgt atgggcggaa tgggtaaaaa aaccggctca agcggcaggc
    16621 gaaagcggct tgttgacgat gtacgaaatc gtcccgggct ttattgtttg cttaatcgtc
    16681 gccgtattgg tttccctgtt caacaaagaa ccttcgcgcg aaacccaaga acgctttgaa
    16741 aaagccgacg cagattaccg cgccgcccga taaacccctt tcagacggca cggcatcccg
    16801 gttcgtgccg tctgaacccg tttccgggtc aaacacaaat aaaccttgcc tgcatacagg
    16861 ccttatttgt gttttacccg tttcattttc aaagaaacgg aagcacctac cctgcccgca
    16921 tttgaagcct tgtatttcat ctgcggattt attacgatat taccgtattc aggccgcacc
    16981 gatgccgcct gccccccgaa aaactttgga gaatccaaaa aatgtttcat tttacatttc
    17041 cggcacaaac tgccctgcgc caagcgataa ccgatgccta ccgccgtaat gaaatcgaag
    17101 ccgtacagga tatgttgcaa cgtgcacaga tgagcgacga agagcgcaac gccgcctccg
    17161 agcttgcccg ccgtttggtt acccaagtcc gcgccggccg caccaaagcc ggcggcgtgg
    17221 atgcgctgat gcacgagttt tcactctcca gcgaagaagg catcgcgctg atgtgtctgg
    17281 cagaagccct gctgcgtatc cccgacaacg ccacgcgcga ccgcctgatt gccgacaaga
    17341 tttcagacgg caactggaaa agccatttga acaacagccc ttccctcttc gtcaacgccg
    17401 ccgcctgggg cctgctgatt accggcaaac tgaccgccac aaacgacaga caaatgagtt
    17461 ccgcactcag ccgcctgatc agcaaaggcg gcgcaccgct catccgccaa ggcgtaaatt
    17521 acgccatgcg cctgttgggc aaacagttcg taaccggaca gaccattgaa gaagccctgc
    17581 aaaacggcaa agaacgcgaa aaaatgggct accgcttctc cttcgatatg ttgggcgaag
    17641 ccgcctacac ccaagccgat gccgaccgct actaccgcga ctatgtcgaa gccatccacg
    17701 ccatcggcaa agatgcggca ggacaaggcg tttacgaagg caacggcatt tccgtcaaac
    17761 tttccgccat ccatccgcgc tactcgcgcg cccaacacgg ccgtgtgatg ggcgaactgt
    17821 tgccgcgcct gaaagagctg ttccttttgg gtaaaaaata cgatatcggt atcaacatcg
    17881 atgccgaaga agccaaccgt ctggagctgt ctttggattt gatggaggct ttggtttcag
    17941 accctgactt ggctggctac aaaggtatcg gtttcgttgt ccaagcctac caaaaacgtt
    18001 gtccgttcgt tatcgactac ctgattgacc tagcccgccg caacaaccaa aaactgatga
    18061 tccgcctcgt caaaggcgcg tattgggaca gcgaaatcaa atgggcgcaa gtggacggct
    18121 tgaacggcta tccgacctac acccgcaaag tccacaccga catctcctac ctcgcctgcg
    18181 cgcgcaaact gctttccgcg caagacgcgg tattcccgca atttgccacg cacaacgcct
    18241 acaccttagg cgcaatctac caaatgggca aaggcaaaga ctttgaacac caatgcctgc
    18301 acggcatggg cgaaaccctg tacgaccaag tcgtcggccc gcaaaactta ggccgccgcg
    18361 tgcgcgtgta cgccccggtc ggcacacacg aaaccctgct cgcctacttg gtgcgccgcc
    18421 tgttggaaaa cggcgcgaac tcgtctttcg tcaaccaaat cgtcgatgaa aacatcagca
    18481 tcgacacgct catccgcagc ccgttcgaca ccatcgccga acaaggcatc cacctgcaca
    18541 acgccctgcc gctgccgcgc gatttgtacg gcaaatgccg tctgaactcg caaggcgtgg
    18601 acttgagcaa cgaaaacgta ttgcagcagc ttcaagaaca gatgaacaaa gccgccgcgc
    18661 aagacttcca cgccgcatcc atcgtcaacg gcaaagcccg cgatgtcggc gaagcgcaac
    18721 cgattaaaaa tcctgccgac cacgacgaca tcgtcggcac agtcagcttt gccgatgccg
    18781 cgcttgccca agaagcggtt ggcgcagccg ttgccgcgtt cctcgaatgg agtgcgacac
    18841 ctaccgccga acgcgccgcc tgcctgcgcc gtttcgccga cctgctcgaa cagcacaccc
    18901 ccgcgctgat gatgcttgcc gtgcgcgaag cgggcaaaac gctgaacaac gccattgccg
    18961 aagtgcgcga agccgtcgat ttctgccgct actacgcaaa cgaagccgaa cacaccctgc
    19021 ctcaagacgc aaaagccgtc ggcgcgattg tcgccatcag cccgtggaac ttcccgctcg
    19081 ccatctttac cggtgaagtc gtttccacat tggcggcagg caacaccgtt atcgccaaac
    19141 ctgccgaaca aaccagcctg attgccggtt atgccgtttc cctcatgcac gaagccggca
    19201 tcccgacttc cgccctgcaa ctcgtcctcg gcgcaggcga cgtgggtgcg gcattgacca
    19261 acgatgcccg catcggcggc gtgattttca ccggctcgac cgaagtggcg cacctgatca
    19321 acaaagccct tgccaaacgc aacgacaatc ccgtcctgat tgccgaaacc ggcggacaaa
    19381 acgccatgat tgtcgattcc accgcacttg ccgagcaagt ctgcgccgac gtattgaact
    19441 ccgccttcga cagcgcggga caacgctgct ccgccctgcg cattttgtgc gtccaagaag
    19501 acgttgccga ccgtatgctc gacatgatca aaggcgcgat ggacgaactc gtcgtcggca
    19561 aaccgattca gctcactacc gatgtcggcc ccgtcatcga tgccgaagca cagcaaaacc
    19621 tgttgaacca catcaacaaa atgaaaggcg ttgccaagtc ctaccacgaa gtcaaaaccg
    19681 ccgccgatgt cgattccaaa aaatccacgt tcgttcgccc catcctgttt gaattgaaca
    19741 acctcaacga actgcaacgc gaagtcttcg gccccgtcct gcacgtcgtc cgctaccgcg
    19801 ccgacgaact cgacaacgtc atcgaccaaa tcaacagcaa aggctacgcc ctgacccacg
    19861 gcgtacacag ccgcatcgaa ggcacggtac gccacatccg cagccgcatc gaagccggca
    19921 acgtttacgt caaccgcaac atcgtcggcg cagtcgtcgg cgtacagccc ttcggcggac
    19981 acggtctgtc cggcacaggc cccaaagcag gcggttcgtt ctacctgcaa aaactgaccc
    20041 gcatccccga atgggttgcc ccgaccctga gccaaatcgg acaggcggac gaagccgcac
    20101 tcaaacgcct cgaagcactg gttcacaaac taccgttcaa cgccgaagag aaaaaagccg
    20161 cagcggccgc tttgggacac gcccgcatcc gcaccctgcg ccgtgccgaa accgtcctta
    20221 ccggcccgac cggcgagcgc aacagcatct catggcacgc gccaaaacgc gtttggatac
    20281 acggcggcag cacggttcaa gcctttgccg cactgaccga acttgccgcc tccggcgtac
    20341 aggcagtggt cgaacctgac agcccctggg cttcctacac cgccgacttg gaaggtctgc
    20401 tgctggtcaa cggcaaaccc gaaaccgccg acatcagcca cgttgccgcc ctgtcgcctt
    20461 tggacagcgc gcgcaaacag gaacttgccg cccacgacgg cgcactcatc cgcatcctcc
    20521 cttcggaaaa cggactcgac atcctgcaag tgtttgaaga aatctcttgc agcgtcaaca
    20581 ccacagccgc cggcggcaac gccagcctga tggcggttgc cgactgattt taccgaaatc
    20641 cccgagcgcg gcccgtgaac caatgccgtc tgaaaacctt tcagacggca
//
LOCUS       DAXHSJ010000020        20161 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000020 DAXHSJ010000000
VERSION     DAXHSJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20161
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.020"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(133..1413)
                     /locus_tag="ACFM8U_000536"
     CDS             complement(133..1413)
                     /locus_tag="ACFM8U_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC6333823.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADFEAYAAKLGEWKL"
     gene            complement(1635..2255)
                     /gene="tmk"
                     /locus_tag="ACFM8U_000537"
     CDS             complement(1635..2255)
                     /gene="tmk"
                     /locus_tag="ACFM8U_000537"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC6333824.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRNLDEVRNSIEKVLDGHFGC"
     gene            complement(2315..3310)
                     /gene="mltG"
                     /locus_tag="ACFM8U_000538"
     CDS             complement(2315..3310)
                     /gene="mltG"
                     /locus_tag="ACFM8U_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC6333825.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3394..3966
                     /gene="ampD"
                     /locus_tag="ACFM8U_000539"
     CDS             3394..3966
                     /gene="ampD"
                     /locus_tag="ACFM8U_000539"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC6333826.1"
                     /translation="MDNHAEAHWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            4160..5446
                     /locus_tag="ACFM8U_000540"
     CDS             4160..5446
                     /locus_tag="ACFM8U_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC6333827.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETVLEESGIIDNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5594..8119
                     /gene="ligA"
                     /locus_tag="ACFM8U_000541"
     CDS             5594..8119
                     /gene="ligA"
                     /locus_tag="ACFM8U_000541"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC6333828.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYF
                     QELGFSLPNGNFGCFKNIDEVLAFYGHMQQKRPELPYEIDGMVVKVNSLAQQHELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLYKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQTPSED
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPT
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQTDKALPTDWQAWRSETQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQALIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            8176..9351
                     /gene="hemW"
                     /locus_tag="ACFM8U_000542"
     CDS             8176..9351
                     /gene="hemW"
                     /locus_tag="ACFM8U_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC6333829.1"
                     /translation="MHAISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALENAGFIHYETSAFAKPAMRCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQNRPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETNPAVFRPTERGRLFLNDLLQCFL"
     gene            9842..10369
                     /gene="nspA"
                     /locus_tag="ACFM8U_000543"
     CDS             9842..10369
                     /gene="nspA"
                     /locus_tag="ACFM8U_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC6333830.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKQVPSTDFKLYSIGASAIYDFDTQSPVKPYL
                     GARLSLNRASADLGGSDSFSQISTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNNVK
                     NVRTGELSAGVRVKF"
     gene            10505..10894
                     /locus_tag="ACFM8U_000544"
     CDS             10505..10894
                     /locus_tag="ACFM8U_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6333831.1"
                     /translation="MSKTVIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPTTMTVF
                     GNGDFRAEARRVFKNLQAVAEAAGGSLDDIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            10971..11801
                     /locus_tag="ACFM8U_000545"
     CDS             10971..11801
                     /locus_tag="ACFM8U_000545"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC6333832.1"
                     /translation="MAHYAIGDIQGCFDELTALLDKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGTLKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPLYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            11816..13273
                     /locus_tag="ACFM8U_000546"
     CDS             11816..13273
                     /locus_tag="ACFM8U_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC6333833.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILATACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFASHFAAFSSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFIESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPASNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(13314..13703)
                     /locus_tag="ACFM8U_000547"
     CDS             complement(13314..13703)
                     /locus_tag="ACFM8U_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333834.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(13885..14286)
                     /locus_tag="ACFM8U_000548"
     CDS             complement(13885..14286)
                     /locus_tag="ACFM8U_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333835.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(14703..15032)
                     /locus_tag="ACFM8U_000549"
     CDS             complement(14703..15032)
                     /locus_tag="ACFM8U_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333836.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVISLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(15058..15288)
                     /locus_tag="ACFM8U_000550"
     CDS             complement(15058..15288)
                     /locus_tag="ACFM8U_000550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333837.1"
                     /translation="MKSRAPTNGQAALDNSVQVKSTSPRRVGVDKANNEIVVLDKTQT
                     FNNGSAEYHGHVRSWQDLHTDQKNALKKAGLD"
     gene            complement(15349..15765)
                     /locus_tag="ACFM8U_000551"
                     /pseudo
     CDS             complement(15349..15765)
                     /locus_tag="ACFM8U_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
     gene            complement(15900..16442)
                     /locus_tag="ACFM8U_000552"
     CDS             complement(15900..16442)
                     /locus_tag="ACFM8U_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC6333838.1"
                     /translation="MINHIEKYIGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LELSKHDLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVIYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVDNLSDMNRKPFVW"
     gene            complement(16569..16796)
                     /locus_tag="ACFM8U_000553"
     CDS             complement(16569..16796)
                     /locus_tag="ACFM8U_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333839.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            complement(16855..17178)
                     /locus_tag="ACFM8U_000554"
     CDS             complement(16855..17178)
                     /locus_tag="ACFM8U_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC6333840.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQNEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            complement(17181..17921)
                     /locus_tag="ACFM8U_000555"
                     /pseudo
     CDS             complement(17181..17921)
                     /locus_tag="ACFM8U_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
     gene            complement(18055..18474)
                     /locus_tag="ACFM8U_000556"
     CDS             complement(18055..18474)
                     /locus_tag="ACFM8U_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HFC6333841.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
     gene            complement(18471..19208)
                     /locus_tag="ACFM8U_000557"
     CDS             complement(18471..19208)
                     /locus_tag="ACFM8U_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC6333842.1"
                     /translation="MVKTADGYKAIAHIRAGESVLSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLLSENGRTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            complement(19357..19587)
                     /locus_tag="ACFM8U_000558"
     CDS             complement(19357..19587)
                     /locus_tag="ACFM8U_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC6333843.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            complement(19602..>20161)
                     /gene="mafB"
                     /locus_tag="ACFM8U_000559"
     CDS             complement(19602..>20161)
                     /gene="mafB"
                     /locus_tag="ACFM8U_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6333844.1"
                     /translation="FNVAAAAKVAKLAKAAKPGKAAVSGDFSAAYNTRTTRKVTTETE
                     GLNRIRQNQKNSNIHEKNYGRDNPNHINVLSGNSIQHILYGDEAGGGHLFPGKPGKTT
                     FPQHWSASKITHEISDIVTSPKTQWYAQTGTGGKYIAKGRPARWVSYETRDGIRIRTV
                     YEPATGKVVTAFPDRTSNPKYNPVK"
BASE COUNT         4923 a         5561 c         4552 g         5125 t
ORIGIN      
        1 aatcggttcc gtactatttg tactgtctgc agcttcgtcg ccttgtcctg atttttgtta
       61 atccactata tcaatctgac agcaaaatac cgcctgaaaa cagttcagac ggcattttaa
      121 accgcaaacg gcttacagct tccattcgcc cagcttggcg gcgtaggctt caaagtcggc
      181 aatcggacgg gttgccacgc cgctttccat cgctgctttg gcggcagccg tagcaacgcg
      241 cggcagcagg cgggaatcga acggagtcgg aatcaggtat tccgcgccga attcaaattt
      301 cttaccgtaa gcggcaacca cttcttcggt cacttcttcc atcgccaaat ccgccaaagc
      361 atacacgcag gcgcgtttca tttcttcgtt gatggtggtc gcgccgacat ccaacgcgcc
      421 acggaagatg aacgggaagc acaatacatt gttgacttgg ttcgggaagt cggaacggcc
      481 ggtaccgata accacgtccg gacgggtttc ttttgccagc ggcggcagaa tttccggatt
      541 cgggttggcc atggcgaaca cgatgggttt ttcgttcatg gtgttcagca tttcaggcgt
      601 cagcaggttc gcgccggaga ggcccaagaa gatgtctttg cctttgacgg catcggcaag
      661 cacgcgctgg ccgttgtctt caatggcgta gaactgttta gactcatcca tgcggtcttt
      721 gtcttcgcgg gtttggtaaa tcacgccttt ggagtcgcaa acggtcacgt tttcgcgttt
      781 caagcccaaa tccagcaatt ggttcaagca ggcaatcgcg gccgcacctg cgccggaaca
      841 caccaaagtc gcttcttcga ttttacggcc ggtaaaacgc agggcgttca atacggcggc
      901 ggcggtaatg atggccgtgc cgtgctggtc gtcgtggaat acggggattt tgcagcgttt
      961 gcgtaattcg cgttcgatgt agaaacactc gggtgctttg atgtcttcga ggttgatgcc
     1021 gccgaaggtc ggctctaaag cggcgatgat gtccacgagt ttttgcgggt ctttttcatc
     1081 gatttcgatg tcgaacacgt ccacaccggc gaattttttg aacaatacgc ctttgccttc
     1141 catcacgggt ttgcccgcca gcgcgccgat gtcgcccaag cccaaaacgg ccgtaccgtt
     1201 ggaaatgaca gcgaccaagt tgcctttggc ggtgtatttg taggcatttt gcggatcggc
     1261 atggatttcc atacaaggag cggctacgcc cggagagtac gccaacgcca aatctttgtc
     1321 ggtcgccaga gatttggtcg gggtaacgga aattttgccc ggaacgggta attcgtggaa
     1381 cttgagggcg gcttctttca atgagttttc catgtgtcaa tcctgttgcg agaaaaagat
     1441 ttgaatcgga ctgtccgaaa cgctttcggg tcatcatgtt acgggcggtt cgttaatatt
     1501 gccgacgcat cctgccttgt aaccgagcgt aattctatca tgccgaaagt ttggcgcata
     1561 ccgccatttt cacctgcggc actgccgttt gattttcggc gcgggcggat gcgcttgttt
     1621 caatatttgc cgcatcagca gccgaaatgt ccgtccaaca ctttttctat gctgtttctg
     1681 acttcatcca agttgcggtt actgtcgata acggcgtacc gttcgggaca ggcggcggct
     1741 cggtcgagat aaacgccgcg cacacgcata aagaaatccg cctgctcctg ctcgaaacgg
     1801 tctttctcgc gcgtctgccc gatacgcgcc atcgacactt cgagtggcac atccaacagc
     1861 agggtcaaat cggggcgcaa accgccctgc acccaatgtt ccaaaatttc aatgtcttca
     1921 gacggcatcc cccgcccgcc gccctgatag gcgaaggttg catcggtaaa acggtcggac
     1981 acgacatgga tgccgtctga aagtgcaggc aatatcacgt cttcaatatg ctgcattctg
     2041 gcggcgaaca tcatcagtgt ttccgcacgc aaaccggctt tggtttcagg gttgagcagg
     2101 atttcgcgca aagcctcacc gaccggcgtt ccgcccggct cgcgcgtgaa cagcacgggc
     2161 agccccctcc gttcaaacca tgccttgatg acggcaaggt tggtggattt gccggcaccg
     2221 tctatgccgt ccaaagtgat gaattgcggt ttcatgcttg cagttccggt aagggtatgc
     2281 cgtctgaaaa cacaaacttt tcagacggca tggtttattt tttcaaaata tatttgcgga
     2341 cggcggcatt gtgttcggtc aaatcatggc tgaactggct caagcccgta ccgtccattt
     2401 tagaaacaaa ataaaggtat ttttcgccag acggatgggc ggcggcatca agcgcggcct
     2461 tgccgggcag cgcaatcggg gttggcggca gaccgccgcg cgtgtaggtg ttgtacggcg
     2521 tgtcgcggcg caggtcggct ttacggattt tgcccttgta tgccgcaccc atgccgtaaa
     2581 tcacggacgg gtcggtttgc agacgcatac cgattttcag ccggttgacg aagacggaag
     2641 cgacatggtc gcggtcggct tcatgccccg tttccttttc gaccaggctc gccataatca
     2701 gcatttcata aggattttta taaggcagcc cgtcctgcct gctttcccat gcctcattca
     2761 ggcggcgttg catcgccttg taggcggttt ggtaaatccg taaatcgctg ccgcccgcat
     2821 cgatttcgta gctgtcgggg aaaaactgcc cttcaggatt gccgctgaag gcatcagggg
     2881 caacttccgc catcagtttt tcattgctcc agcctttggt gtcgtgttcg atgtcgggcg
     2941 ttgcgtcgat gactttcctc atatgcgaaa aacgcgaacc ttcgataatc tgcacggtaa
     3001 cggaatccgg cctgccgccg cgcattttct gcaagatatc ccaagcagac acttccgaag
     3061 gcaatctgta cgtccccgta tgcagcctgt tgtgcacacc caaaacgtag gccgccgccg
     3121 tcaaaacatg cctgctgaac acgatgcggt cttcggcaag tttcctgccg accgacgaaa
     3181 taccctggtt tttggcaatt ttgattcggt atgccctgcc gttatcctta ggaacaaaaa
     3241 gcagcgcggc gaaaacggct gccgacacgg tcaaaaaaac ggcagaccat ttcaacaatt
     3301 ttctcaacat ggtaggattc ccagcattcg gaatacgtct taaaattcgg gcgacagtat
     3361 aacccaaatt gcccgccgga caggaagaac atcatggaca atcacgccga agcacactgg
     3421 caggacggtt ggctgcaaag cgtgcgccac attccgtcgc cgaatttcgg cccgagggag
     3481 tccaaagaag aggtttccct tgtcgtattg cacaatatct cgctgccgcc gttcgaatat
     3541 ggtacggatg cggtggaaaa gctgtttgcc aaccggctcg accccgacgg acatccgttc
     3601 ttcagcctga tacacacttt gcgcgtatcc agccattttt taatcaaacg cgacggcgaa
     3661 acggtacagt tcgtttcctg cgacaatatg gcataccatg ccggcgtatc ctcgtttcgc
     3721 ggacgggaaa aatgcaacgc attttccatc ggcatcgaac tggaaggctg cgatttcgaa
     3781 ccctttaccg aagcgcaata ccgttcgctc gaagccttgt tggacgcaat ctgccgccgc
     3841 taccccatca cggcggtaac ggggcaccaa gacatcgcgc ccggccgcaa aaccgacccc
     3901 ggtcattttt tcgactggcg gcggatacgg gggaaagggt ttcccgtgga cagaaatgcc
     3961 gtctgaaaac cacagatttt agacggcatt tcggaaaatt tccgcttatt tcgacacact
     4021 gctgtcagac cctgcaattt tcgttataat tcaaacgtta taaccggccg ttccgtcaga
     4081 ctgccggaaa cgcaagcccg atttaaccgc agtacccgca ctttgcgcat cctgcatccc
     4141 gaaatttaaa gtgattgcca tgatttacat cgtactgttc ctcgccgccg tcctcgccgt
     4201 tgtcgcctac aatatgtatc aggaaaacca ataccgcaaa aaagtgcgcg accagttcgg
     4261 gcactccgac aaagatgccc tgctcaacag caaaaccagc catgtccgcg acggcaaacc
     4321 gtccggcggg ccagtcatga tgccgaaacc ccaaccggcg gtcaaaaaaa cggcaaaacc
     4381 ccaagacccc gccatgcgca acctgcaaga gcaggatgcc gtctacatcg ccaagcagaa
     4441 acaggcaaaa gcctccccgt tcaaaaccga aatcgaaacc gtcttggaag aaagcggcat
     4501 tatcgacaac tccgcccaca ccgtttccga accccaaacc ggacattccg caccgaaacc
     4561 tgccgacgcg ccggcaaaac ccgttcccgt tccgcaaacg ccggcaaaac cgctgattac
     4621 gctcaaagaa ctgtcaaaag tcgaattacc ctggtttgac gtgcgcttcg acttcatctc
     4681 ctatatcgcg ctgaccgaag ccaaagaact gcacgcactg ccgcgccttt ccaaccgctg
     4741 ccgctaccag attgtcggct gcaccatgga cgaccatttc cagattgccg aacccatccc
     4801 gggcatccgc tatcaggcat ttatcgtggg tattcaggca gtcagccgca acggacttgc
     4861 ctcgcaggaa gaactctccg cattcaaccg ccaggtggac gcattcgcac aaagcatggg
     4921 cggtcagacg ctgcacaccg accttgccgc ctttatcgaa gtggcttccg cactggacgc
     4981 attttgcgcg cgcgtcgacc agaccatcgc catccatttg gtttccccga ccagcatcag
     5041 cggcgtagaa ctgcgttccg ccgtaacggg cgtgggtttc gttttggaag acgacggcgc
     5101 gttccactat accgacacgt cgggctcgac catgttctcc atctgctcgc tcaacaacga
     5161 gccgtttacc aatgcccttt tagacaacca gtcctacaaa ggcttcagta tgctgctcga
     5221 catcccgcac tctcctgcag gcgaaaaaac cttcgacgat ttgtttatgg atttggcggt
     5281 acgcctgtcc ggccagttga acctgaatct ggtcaacgac aaaatggaag aagtttcgac
     5341 ccaatggctc aaagacgtac gcacttatgt attggcgcgt cagtccgaga tgctcaaagt
     5401 cggtatcgaa ccgggcggca aaaccgccct gcgcctgttc tcctaaaatc gaatgcaatc
     5461 aaagcggata tgtcgttacg gcatatccgc tttttttcgg gtaaaattca gctgttttca
     5521 acctatcatt tttcagacga cctttgcccg tttcatcccg tatcagacaa aggtcgtctg
     5581 aaaaccttct ctcatgaatc caaactcaaa acataacact aactttacca atcttcttaa
     5641 accgtctgat agtgatataa aacaatttgc cgcacaacac atccgccacc tcaccgacct
     5701 gctcaaccgc tatgcctacg aatactacac cctcgacgcg cccagcgtac ccgatgccga
     5761 atacgacaaa ttattccgcg aactcgaagc gttggaatta aaccatcccg agctcaaact
     5821 gcccgacagc ccgacccagc gcgtcggcgg cgagcctttg gcaggctttg ccgaagtgcg
     5881 ccacgaagtg ccgatgctgt cgctgaccaa cgctttttcc ccgcaagatg aaaacggcgt
     5941 gttcgaccat gccgaaatgt acgctttcga ccaacgcgtg cgcgacggtt tggacggcgg
     6001 caatcccgaa tacgttatcg aacccaaatt cgacggcctc gccatcagcc tgctctaccg
     6061 cgacggcgta ttggtgcagg cggcaacgcg cggcgacggc acaacgggcg aagacgttac
     6121 ccaaaacatc aaaaccgtat ccaacatccc cttgcggcta cacggcgaaa atacgcccga
     6181 actcatcgaa gtacgcggcg aagtactgat gcaaaaagcc gattttgccg ccctcaataa
     6241 aagacaagcc gaaaacgggc aaaaaccctt tgccaatccg cgcaacgccg ctgccggcag
     6301 cctgcgccaa ctcgattcgc gcatcactgc gcaacgcaaa ctgcactttt tcccctactc
     6361 cgtcgcccgc cagcaagatg gtttcgtcgc ggaagaacac atccaggaac tcgcctattt
     6421 ccaagaactc ggcttcagtc tgcccaatgg caatttcggc tgtttcaaaa atatcgatga
     6481 agtattggcg ttttacggac acatgcagca aaaacgcccc gaactgccct atgaaatcga
     6541 cggcatggtc gtcaaagtca acagcttggc gcaacagcac gaactcggct tcatctcccg
     6601 cgcgccgcgc tgggcgattg cccacaaatt ccccgccgaa gaagccctga ccattgtcga
     6661 agcgattgac gtgcaaatcg gcaggacggg cgcggtaacg ccggttgccc gcctgcagcc
     6721 cgtattcgtc ggcggcgtaa ccgttaccaa cgccaccctg cacaatcagg acgaagtatc
     6781 gcgtaaagac gtgcgcgtcg gcgataccgt cgtcgtgcgc cgcgccggag acgtgattcc
     6841 cgaagtcgtg cgtgtgattt ttgaacgccg cccgatgcag gaaaccgccg ttgctgtttc
     6901 agacggcatc gggcatcggc aggacgattt gtttgccgaa gcaccggctg caaaccaaac
     6961 ccaatccgtt ccgctctaca agccctaccg cctgccgacc cactgcccca tctgccgcag
     7021 cgaaatcgaa cgcgaagaag gcgaagccgt cgcccgatgc agcggcggca tgctttgtca
     7081 ggcacaacgc gcgcaaggct taatccactt cgcctcgcgc aaagcgatgg acatcgacgg
     7141 cttgggtgaa aaacaaatcg aacagcttgt cgcccaagac ctcgtccgcc acttcgccga
     7201 cctctaccgc ctcgatattc cgaccctgca aaaaatgaag gaaacggcgg acaaaaccgt
     7261 tgccgaatcg gatcaaacgc cgtctgaaga cagttcggtc ggcgcgtcgg gcaaacataa
     7321 aaaacaaccg accaagtggg cggaaaacat cctcgcaggc atagaatccg gcaaaacacc
     7381 cgaactcgcc cgcttcctgt tcgcgctcgg catccgccac gtcggcgaac gcaccgccaa
     7441 aacgctggca caggcattcg gcacattgga acgcgtccgc cgcgcccccg aacccgtcct
     7501 cgcctgcctg cccgacatcg gtacggtagt ggcgcgttcc attgcccact tcttcgccca
     7561 agccgaacag caggcgatga tagacgagct gctcgccgca ggcgttgccc cgcaagccca
     7621 agccgtcagc ctgcctaccg cacaatatgc cgaaccgcaa cgctggattg cccgcctgcc
     7681 cggtttcaaa atcagcgaaa acaaagccca agccttatgg gaactcgccg gcaaaaacat
     7741 agaagggctg caaaccgaca aagccctccc caccgactgg caggcgtggc gcagcgaaac
     7801 acaaaacgcc gccctgctcg aaaacctgaa aaccttcttc gcgcaaatgc cgtctgaaga
     7861 tgaggcggcg caaggttcag acggcatcaa taaagccgtg gcgggcaaaa ccttcgtgtt
     7921 aaccggcaca ctgcccaccc tcaaacgcga ccaagcccaa gccctaatcg aagccgcagg
     7981 cggcaaagtt tccggcagcg tttccaaaaa aaccgattac gtcgtcgccg gagaagccgc
     8041 gggcagcaag ctggaaaaag ccaatgcctt gggcgtttcc gtcctcagtg aagccgaact
     8101 gcttaccctg ctcggctgaa cctgtgccgt ctgaaccgcc ttcagacggc attgtccact
     8161 tttccgaacc acaccatgca cgccatttcc tttccaaacc gcacccggct caccgccctg
     8221 ccgcccctgt cgctctatat ccacatcccg tggtgcatca aaaaatgccc gtattgcgac
     8281 tttaattccc acagcctgaa aaacggattg cccgaagccg cctatatcga cgcgctgctg
     8341 accgacttgc agcttgaatt gcccaatatt tggggcaggc cggtggaaac gatatttttc
     8401 ggcggcggta cgcccagcct gtttcaggcg gaatcgattg accgtttatt gagcggcgtg
     8461 cgttcgctgt tgcgcttgca gcccgaagcg gaaattacct tggaagccaa tccggggaca
     8521 tttgaaatcg agaagtttca aggatttaaa gacgcaggca tcacgcggct ttctatcggc
     8581 gtacagagtt tcaacgacga tatgctctca aggctggggc gcgtccacaa cggcaaggaa
     8641 gccttaaccg ctatcgatac cgccttaaaa ttatttgata aagtcaatat cgacttgatg
     8701 tacgccctac cgaaccaaac cgttcagacg gcattggacg acgtacaaac cgccatcgct
     8761 acgggtgcga cccacatcag cgcgtatcat ctgacgatgg aaccgaacac gccgttcggt
     8821 catacgccgc ccaaaggttt gccgcaagac gaagccgccc tcgacatcga agacgcggta
     8881 cacggcgcat tggaaaacgc cggctttatc cattacgaaa catcggcttt tgcgaaaccc
     8941 gccatgcggt gccgccacaa tttgaactac tggcagttcg gcgattattt gggcataggc
     9001 gcgggcgcgc acggcaaaat ttcctacccc gaccgcatcg agcgcaccgt ccgccgccgc
     9061 catcccaacg actacctcgc cctgatgcaa aaccggccgg gtgaagccgt cgaacgcaaa
     9121 accgttgccg ccgaagattt gcctttcgag tttatgatga acgccctgcg cttgaccgac
     9181 ggcgtacccg ccgcgatgtt gcaggagcgc acgggcgtac cgagtgccaa aatcatggcg
     9241 caaatcgaaa cggcaaggca aaaaggttta ttggaaacca accccgccgt attccgcccg
     9301 accgaacgtg gacgattgtt tttaaacgat ttattgcagt gttttttata agcgcaagca
     9361 aatacaagga tgccgaaacg gcaaattcca acaacggaca ttctcaaaaa tgacgcgcgg
     9421 caacggcggc ggcgacaagg acggaaccgg caggcggcgg ataccggcgg ttttcggtac
     9481 gggggcgtgc cgaagatgcg gggcttatcc ttgccgccgt ggaaaaagcc ggcggcagcc
     9541 gcctcccccg ccgggcgttt cagacggcat cgccgcgcac aaaaaaccgt atgcctgcac
     9601 gcgcgcaaat tccgtcatcc acgccgtccc tccttttccc aaaccgtttg caagttccgc
     9661 ccatccgctg cgtgatgccg ctccgtttaa aggcagggca cggaacaatg cccggtatgc
     9721 cgtcccgcat cttggggcat tgaaaatatt acgatgcaaa aagaaaattt cagtataata
     9781 cggcaggatt ctttaacgga ttcttaacca tttttctccc tgaccataaa ggaaccaaga
     9841 tatgaaaaaa gcacttgccg cactgattgc cctcgccctc ccggccgccg cactggcgga
     9901 aggcgcatcc ggcttttacg tccaagccga tgccgcacac gccaaagcct caagctcttt
     9961 aggttctgcc aaaggcttca gcccgcgcat ctccgcaggc taccgcatca acgacctccg
    10021 cttcgccgtc gattacacgc gctacaaaaa ctataaacaa gtcccatcca ccgatttcaa
    10081 actttacagc atcggcgcgt ccgccattta cgacttcgac acccaatcgc ccgtcaaacc
    10141 gtatctcggc gcgcgcttga gcctcaaccg cgcttccgcc gacttgggcg gcagcgacag
    10201 cttcagccaa atctccaccg gcctcggcgt attggcgggc gtaagctatg ccgttacccc
    10261 caatgtcgat ttggatgccg gctaccgcta caactacatc ggcaaagtca acaacgtcaa
    10321 aaacgtccgt accggcgaac tgtccgccgg cgtacgcgtc aaattctgat atacgcgtta
    10381 ttccgcaaac cgccgagcct ttcggcggtt ttgttttccg ccgccgcaac tacacaagcc
    10441 ggcggttttg tacgataatc ccgaacgctg cggcttctgc cgccctattt ttaaggaatc
    10501 cgaaatgtcc aaaaccgtca tccacaccga caaagcccct gccgccatcg gcgcgtacag
    10561 ccaagccgtc cgcgcaggcg acaccgttta tatgagcggt cagatcccgc tcgatcccac
    10621 caccatgacc gtgttcggca acggcgattt ccgcgccgaa gcccgccggg tctttaaaaa
    10681 cctgcaagcc gtcgccgaag cggcaggcgg ctcgctggac gacatcgtca aactcaacgc
    10741 ctatctgacc gacttgggca acttcgccgt cttcaacgaa gtgatggcgg aatttatcgc
    10801 cgagccgttt cccgcccgcg ccgccgtcgg cgttgcctcg ctgcccaaag gcgtgcaggt
    10861 cgaagccgaa gccgtcctcg ttttgaacgc ataattccac gttcggacgg cataagattt
    10921 ccaacagtgc cgtccgaacc gcaacacttt cccacttcac aacacccgca atggcacatt
    10981 acgcaatcgg cgacatacaa ggctgtttcg acgaactgac cgcgctgctc gataaaatcg
    11041 gcttcaacca cggcacggat accctctggc tgacgggcga catcgtcaac cgcggtccga
    11101 aatccctcga aacactgcaa ttctgcatcc ggcacgaaaa cagcgtgcaa atcgtcctcg
    11161 gcaaccacga cctgcacctg ctcgccgtcg gctgcggcga aggcactctc aaacgcagcg
    11221 acacaatcga acccatactc aaacaccccg acgggaaaaa aatgctcgac tggctgcgcg
    11281 cgcaaccgct gttgatacgc gagggcagcc gcgtgatggt acacgccggc atcctgccgc
    11341 aatggcgcat cgccaaagcc gaatcgctcg ctagagaagc cgaagccgaa ctgcgcggca
    11401 aaaaatacgt caaattcttc tccaaaatgt acggcaacaa accggccgcg tgggacgaag
    11461 gtttggaagg ctatgcccgc ctgcgcttca ttgtcaacgc cttcacgcgg atgcgcgccc
    11521 tgacctttaa aaacgaactg gatttcgact acaaatccac agtgaaaaaa atgccgcttt
    11581 acctgcgccc gtggttcaaa gcccccgacc ggcaaaacct cgaccatacc atcatcttcg
    11641 gacactggtc ctcgctgggc tacacgaatg ccgacaacgt catctcgctg gacaccggcg
    11701 cgctgtgggg cggacagctg accgccgtca acctcgaaac ggaagaaatt acccaagtcc
    11761 aagccgccgg cggcatagac tggaaaagct tcgcaaaata acggaccgcc ccactatgca
    11821 caaaatcctg cccatcgccc acgtcctctc ccgcctgggt atgctgtttt cctttatcct
    11881 gctgataccc gccgccctct cctacgcctt ttcggacggc gcgtacaccg ctttcgccac
    11941 caccgcgacc gttacccttt ccggctcgtg catcgtccgg ctcgccaccc tccggttcag
    12001 gcgcgaactg cgcccgcgcg acggcttcac cctcgtcctg atgttgtggc tggcgtttgc
    12061 cgctatggct gcgatgccga tgtacctgta tttcccgaat atgggcttta ccgacgcatt
    12121 ttttgaatcg atgtcgggac tgaccaccac cggcgcgacc gtcatccccc acgtcgacgg
    12181 actcgccccc tccgtcaact tttggcggca tatgctcaac tggctgggcg ggatgggcat
    12241 catcgtcctt gccgtcgcca tcctgcctat gctcggcgta ggcggcacgc aattattcaa
    12301 agccgaaatc cccggcattg acaaagaaag caaaatgtcg ccgcgcattt cccaagtggc
    12361 gaaaaaactc tggttcggct acaccctgat taccatcctc gcgacagcct gcctgcattt
    12421 tgccgggatg ggctggttcg atgccgtctg tcacgcaatg gcgaccctct cgctgggcgg
    12481 attttccacc cacgatgcca gcatagctta ttacaactcc cccctcatcg aagcagtcat
    12541 catcgttttc accattgtcg gcggcatcaa ttttgccagc catttcgccg ccttcagcag
    12601 ccgcagcctc aaaacctatt ggaaagacga agaatgccgg acgatgctgc tgctgctctc
    12661 cggcagcatc cttgccgccg ccctgtacct gtggcacacc ggctattacg ccggcttcat
    12721 cgaatccctg cgctacaccg ccttcaactt cgtctccatc ggattggcaa acgggctggc
    12781 caacaccgac ttcgcacaat ggccgctcct gatttccctg tggatgtttt tcctcgccaa
    12841 catcctcgcc aactccggct cgaccggcgg cggcatcaaa accatacgcg ccctcgtcct
    12901 gttcaaattc agcctgcgcg aaatgatggt gctgctgcac cccaaagccg tccgcaccgt
    12961 caaaatcagc ggcaaggcca tacccgaccg cctcgcgctg accgttatgt ccttcatctt
    13021 catctacttt atgaccgtcg tcctcttcag cttcctgctg atggcgagcg gtatggaatt
    13081 taccaccgcc ttcaccgccg tcatcgcctg catcaccaac gccggccccg gcctgggcga
    13141 agtcgggccc gccagcaatt acgccggttt ggacgtgatg cagaaatgga tctgcgttac
    13201 cgccatgctc ttgggcagac tggaaatctt caccgtcttc atcctcttca ctcccgccta
    13261 ctggaagaaa taaaaaatgc cgtctgaaaa cttttcagac ggcatcttgt cctttaaaga
    13321 agcggaagct ctacaaaagg aaagaaatgc tgaaacctct gataaatttc aggatatttc
    13381 tctaaggctt ttaatgcttt ttcttttgaa ataggcggat catagacatc tatccccctt
    13441 aagaaggcaa tgcccgttaa tgcatttttt tgatcatttg aaatgtaacc ataattaacg
    13501 actccaatta catcataaat ttcccctata ctcttatcaa aatcaatcaa atcaaaaata
    13561 tacaatggaa ttgtatcaat aggagtatct ctgacaacta tatccaacca taatttaaat
    13621 tcagaaagat taatagcatc atagaacata cagactattg caaatcctaa atcttcacaa
    13681 ttttctttac taattttcca cataattatt tacctttatc cattgcttcc caaccgatac
    13741 atccggtatt ttagattctc ctttgccgtc aaacgctctt ttggttacct gctttgtatc
    13801 aaaaatgccg tctgaaagcc gaatattgtt tcagacggca ttttgattgt tgggttttta
    13861 atcagagctt tgtttgataa tttgttattt tgaataaaat acaggttctg tcgagctgat
    13921 aacttctatt acagaatcgc taaaaaagaa aatataagcc ttataatatt tcttttcaaa
    13981 aaaatcaaaa ctttcttctt tcaaccagtc catataactg tcatctatta tctcgaaaaa
    14041 ataatttata gcaggatttc tatcccaatc atttttttct ttattttcaa ataaatattc
    14101 tgaaaatttt actttataag attcatctgt tatttgaaaa tgaattatat tgtcaataaa
    14161 tatttcaata tttaattcat aatttcggtc ggatgccaaa atgttcacat cttgttcggt
    14221 aacacaaatt ttgtccacat aaaatctgtg attattgtta gaaaattgta atggtttaat
    14281 cttcacaatc gttttccata tctgattttt gttttcttat tgccctgccg gataaagcag
    14341 gtctgcctcc gtactgtcta tcctcacatt tactttcctg ccatcggctt ttatctcccc
    14401 ggttttaccg tttttgccaa cttcgcggct ttggcggcgg caatgttgaa ggcggcttcg
    14461 acggtttcgg cggcattggg gttgacccgt actcatccgc aaacggcaac acgacggacg
    14521 gtgtttgctt ttcagccgtc tgccgttttg accaaggtgc tgccgtggaa ggagcaggtg
    14581 taggattttt gaagaactca gttctcaata tcctatttca aatgaaggaa atgccgtctg
    14641 aaaattaaac acggtttcag acggcatttt gattgttggg ttttcaaccc gacctacggt
    14701 tcctaatgat tttcagataa aaataaagaa atatcttcta acgcatcttc agccctacct
    14761 tgaagctctg gatcatcttg ctttgaatgt agtaaagaaa taactttatc gtaatcaccg
    14821 atatttgaat gtagcctggc gagatgtccg aaacaggtta gcgataatcc ggcaatatcc
    14881 aagtctttat ttttcgataa ttccatcaat ttctcagaaa tccacggtac ttcatatgct
    14941 ttggaattaa ataccatatc aagaatgcca tctataacac tttgtttatc atcagaagaa
    15001 atcaactttt ctgttttact tttatcagtc attttttaat ttttcctttt gaattaacta
    15061 atccaatcct gcttttttta aagcattttt ctgatcggta tgcaaatctt gccaacttct
    15121 gacatgcccg tgatattccg cagaaccgtt attaaaagtt tgagttttgt ctaatacaac
    15181 gatttcatta ttggctttat caaccccaac tcttcgagga gaagttgatt taacttgaac
    15241 ggaattatca agagctgctt gtccgtttgt tggtgctcta cttttcacag aattatcatt
    15301 tttgccatga taagaagcgt ctttatatcg ttggttgcct ttatcatacg gacaatcatt
    15361 atgaacccaa accccttccg tttccgcctg actgcccttg acgaagtaag tatgccaatc
    15421 ggcaacggtc agattgtagg ctttgagcgg ttttggtttg acaacggtgt tgcggacggt
    15481 ttgggttctg ccgttttcgg ataacagtct gcttcccgcc tttaagtctt ccgccttaat
    15541 ccatttgccg tccgaataaa acgggtggat gcggttggaa atcagggttt ggctgttgcc
    15601 gatgccgtct gaaacttcaa tgtaaacggt ttcccgatac ggattgccgt atcgggcggt
    15661 aacgggtttg tatcctgttt ccccgcttgc ctcgtccttg gaaaggacgc ggtctccggc
    15721 ttgaatatgg gcgatggctt tgtagccgtc tgccgttttg actaaagcac cgacgtataa
    15781 gggacggaga caggattttt gaagaactta gttcccaata tcctgtttca aatgaaggaa
    15841 aggttgtctg aaagttaaac acggtttcag acgaccttta atcaaattta agaaagccct
    15901 taccaaacaa acggtttccg attcatatcc gaaaggttgt caacttcatt atccagcaag
    15961 aactgctcaa aagcattcca accctttttt tccaccaatt cggcttcctg tttatacaag
    16021 gggacaagca aagggaaaac gatattgtag tgttcgccat aacagacctg aaaatcatcg
    16081 tcgaaataaa atggggcgga aacatacagt gcatccattt ttgttgaatc aacaatgctt
    16141 ctaggcaaat aaataacctg tcctcgaaga aggatttttt tattctcaat gatggtttct
    16201 gccagccatc ttaaaaatgg gaaaatctga ttttcatcat attttaaaga acatacaaaa
    16261 agtatttcaa atctgctttt ataattcaga tcatgtttgc ttaactctaa agtaatataa
    16321 gtttttaaat ccctgttagg ggaagaggga atagctgcga tatttaattt gtactgactt
    16381 cccgaatcgc ttttcgagcc atgagaaata gtgcctatat atttttcaat atgattaatc
    16441 atatttttct tagcttccca tcactgtatc gaataatcat agaccagatt gagacaaaac
    16501 gccttgttcc cattgataga aagtccgtct gaaaagtaaa agcgtttcag acggactacg
    16561 tttgccgact atccgaccca ctttattatc caatgagatt cttgcaggta ttcttttaat
    16621 atttctaagt tttcgaccca gtcatcgtaa ctgtcaaatg atgagaattt gtcatttgaa
    16681 gataacagta tcagaaatcc cccggtattt tcctgatcat caataatttt tatgaatttt
    16741 tcttcaggat tgaccccatt caggataacc cccaaagtat tgaattttat gtccatagac
    16801 acaccctaat ctttaatggg aatatggttt tttcaaccca acctacactt tttgttagtt
    16861 cccatacgaa ccaaaacctt cataattaca atcatttttt tgtgcaatac caaataacgt
    16921 agtttcaata gctaaaatgt tttcaaggga agggacgata ttgacggaac aatagcaagt
    16981 ccacatttct ccgaattcat tttgctcaat actgatttga aaatcttgcc gataagaaat
    17041 gagtcggctt tctattgatt gcagattctt ttgagaacct cctataaaaa actctattat
    17101 cataggtttt gtcaaatcaa accctagttc ttgatattgt ttcagtacat ctgaagtaga
    17161 tttttcgtca acaatcattt ttaatctcca tcttgttttc ggtatttttt ccccgggctt
    17221 tcaattcgat tgtaattatt ggtacttttg gaaccaccgt gagcgtcaat tcttctagat
    17281 tcatctttga atgcctctcg attatttttg gcaggagtaa aatctgtagc tatatgaggg
    17341 tcattattaa ccctagcttc tcttgctcca gagatttggg aacgttgtct tgtgcccttt
    17401 ccagaataag ttcgaccact ttcatgagta ttagtataag aaccaacttc tgtcctatat
    17461 ttttctccat acggacaatc attatgaacc caaacccctt ccgtttccgc cctatcaccc
    17521 ttgacgaagt aagtatgcca gtcggcaacg gtcagattgt aggctttgag cggctgctgt
    17581 ttgaaggtaa tgttttgaac cgtctgtttt gcgccgcttt cggaaagcag ggtgtcgcct
    17641 tttttcagac gacctgcctg tatccatttt ccttgactgt aaaacgggtg gattttattg
    17701 gaaacgaggg tctgtatttt accgatgccg tctgaaactt taatgtaaac ggtttcccga
    17761 tacggattac cgtatcgggc ggtaacgggt ttgtatcccg tttctccgct tgcctcgtcc
    17821 ttggaaagga cgcggtctcc gactcggata tgggcaatgg ctttgtagcc gtctgccgtt
    17881 ttgaccaagg tgctgccgtg gaaggagcag gtgtaggatt tttgaaaaac ttaattccca
    17941 atatcctgtt tcaaatgaag gaaataggtc gtctgaaaat taaacacggt ttcagacgac
    18001 cttttaatcg ttaggtttac aatctaacgt aatccattct tacttataga tcgattaaga
    18061 gaagcccaaa caagcttccc tgtagctgag gatatttgct tcctcatatc tatttaaaat
    18121 tccttttaaa tcagaattat attttttggg cggaaaatta accgataaat aattctctcc
    18181 attttttctg attttaccac tgtgttcttc tatggctgcc aataaatcct ttgggaaatg
    18241 atccaaaaca acgatgttta tcgtggtatt tcctgaagct tttatcaagt ggtcgaaaaa
    18301 cagcatctta tcctcacgtt ctatggcaac aatgtcgttt aaagccaaat tcggtgcgaa
    18361 aagaggaatc tcttgaataa gaaaatttcc atcaggcatt atgtttcctg acagtatttc
    18421 ttctgcaata ttgctttcca aatcgtaata gacgacagta agtttcgatt tcatttgtta
    18481 ctcttcttaa tgttgcagcc tctgcataat acctgcccgt ttttaggtgt accttgacca
    18541 cccttagatt tagggatgac atgatctact tgtctttcat ttgaagtagg agaaatattt
    18601 tttatagatt gtttggctgg aattgtttca ataccgcaat tagcgcactt aacttgacca
    18661 ttattttgaa tcctattcaa atcaataacg gcttcttttc ctgctttagt aaagtctttt
    18721 cctgcccgat cgtttttact aagccgttct gcgcgaagtt ttgctttctc taaattattt
    18781 gaaccgccat acggacaatc attatgaacc caaacccctt ccgtttccgc cttatcgcct
    18841 ttgacaaagt aagtatgcca atcggcaacg gtcaaattgt aggctttgag tggttttggt
    18901 ttgacaacga tgttgcggac ggtttgggtt ctgccgtttt cggataacag tctgcttccc
    18961 gcctttaagt cttccgcctt aatccatttg ccgtccgaat aaaacggatg gatgcggttg
    19021 gaaatcaggg tttgactgtt gccgatgccg tctgaaattt caatgtaaac ggtttcttga
    19081 tagggattgc cgtatcgggc ggtaacgggt ttgtatcccg tttttccact tgcctcgtcc
    19141 ttggagagga cgctctcgcc ggctcggata tgggcaatgg ctttgtagcc gtctgccgtt
    19201 ttgaccaagg tgctgccgtg gaaggagtag gtgtaggatt tttgaagaac ttagttctca
    19261 atatcctgct tcaaatgaag gaaaggtcgt ctgtaaatta aacacagttt cagacgactt
    19321 tttgattgtt gggtttttaa accgacctaa tatttattaa tctgatgtac tttcaattcc
    19381 aaattcctta ttaataaaag agatttcttg ggattctttt tccgtaagct ggatattgaa
    19441 atcttctata taatcacata gcgtttcaaa agctagcaaa cgttctgaaa aagtaatgta
    19501 ctctagtgcg gcatcaatta gattagcgtc aattctatct tcaagagatt ttcctaaatt
    19561 ttttattcgt tcgtctaatg ttttcatctt tcaaatactc attattttac agggttatat
    19621 ttgggattag aggttctatc ggggaatgca gttaccactt ttcctgttgc aggttcataa
    19681 actgttctga tacgaattcc atctctcgtt tcatatgata cccacctggc tggtcttcct
    19741 ttagcaatat atttgccgcc tgttccagtc tgtgcatacc attgcgtttt tggggatgta
    19801 acgatatcac taatttcatg agttatttta ctggctgacc aatgttgggg gaatgttgtc
    19861 ttaccaggtt tgccaggaaa aagatgccca cctcctgctt catctccata cagtatatgt
    19921 tgtatagaat ttccagataa aacattaata tgattaggat tatctcttcc ataatttttc
    19981 tcatgtatat tactattttt ctggttctgt ctgattctat ttaacccctc tgtttctgta
    20041 gtaacttttc tagttgttct tgtattgtat gcagcagaaa aatccccgct aaccgcagct
    20101 ttccccggtt tcgccgcctt tgccagcttc gcgactttgg cggcagcggc aacgttgaag
    20161 a
//
LOCUS       DAXHSJ010000021        19756 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000021 DAXHSJ010000000
VERSION     DAXHSJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.021"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(160..2997)
                     /locus_tag="ACFM8U_000560"
     CDS             complement(160..2997)
                     /locus_tag="ACFM8U_000560"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC6333845.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLF
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFALPEKYAGLDRFAARK
                     QMVADLQEQGLLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLEKLLVKRTTGLRKPETAPKVEEASRKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVDRL
                     NQTIEQVTQAYETYRFDLAAETLYSFMWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADSGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(3094..3903)
                     /gene="zupT"
                     /locus_tag="ACFM8U_000561"
     CDS             complement(3094..3903)
                     /gene="zupT"
                     /locus_tag="ACFM8U_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFC6333846.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTTGMAVIAVSLVLFRF"
     gene            complement(4084..5340)
                     /locus_tag="ACFM8U_000562"
     CDS             complement(4084..5340)
                     /locus_tag="ACFM8U_000562"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC6333847.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETETGRFEADAVVCALGCFSRTVLAQLDLDLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(5419..6810)
                     /locus_tag="ACFM8U_000563"
     CDS             complement(5419..6810)
                     /locus_tag="ACFM8U_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6333848.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(7167..7943)
                     /gene="lpxA"
                     /locus_tag="ACFM8U_000564"
     CDS             complement(7167..7943)
                     /gene="lpxA"
                     /locus_tag="ACFM8U_000564"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC6333849.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(7986..8666)
                     /locus_tag="ACFM8U_000565"
     CDS             complement(7986..8666)
                     /locus_tag="ACFM8U_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC6333850.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(8873..9322)
                     /gene="fabZ"
                     /locus_tag="ACFM8U_000566"
     CDS             complement(8873..9322)
                     /gene="fabZ"
                     /locus_tag="ACFM8U_000566"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC6333851.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFESMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(9358..10401)
                     /gene="lpxD"
                     /locus_tag="ACFM8U_000567"
     CDS             complement(9358..10401)
                     /gene="lpxD"
                     /locus_tag="ACFM8U_000567"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC6333852.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKAHGGI
                     HPTAVVEPSATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVMHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVHIHRLSEMNKRLK
                     TLEQQLSDGKDTQ"
     gene            complement(10434..10934)
                     /locus_tag="ACFM8U_000568"
     CDS             complement(10434..10934)
                     /locus_tag="ACFM8U_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC6333853.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKVQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(11000..13393)
                     /gene="bamA"
                     /locus_tag="ACFM8U_000569"
     CDS             complement(11000..13393)
                     /gene="bamA"
                     /locus_tag="ACFM8U_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC6333854.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(13450..14790)
                     /gene="rseP"
                     /locus_tag="ACFM8U_000570"
     CDS             complement(13450..14790)
                     /gene="rseP"
                     /locus_tag="ACFM8U_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC6333855.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGRKIALTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(14825..16060)
                     /gene="ispC"
                     /locus_tag="ACFM8U_000571"
     CDS             complement(14825..16060)
                     /gene="ispC"
                     /locus_tag="ACFM8U_000571"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC6333856.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIEGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(16065..16862)
                     /locus_tag="ACFM8U_000572"
     CDS             complement(16065..16862)
                     /locus_tag="ACFM8U_000572"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC6333857.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLSGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(16865..17611)
                     /locus_tag="ACFM8U_000573"
     CDS             complement(16865..17611)
                     /locus_tag="ACFM8U_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC6333858.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFSRQILKGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAKGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(17667..18224)
                     /gene="frr"
                     /locus_tag="ACFM8U_000574"
     CDS             complement(17667..18224)
                     /gene="frr"
                     /locus_tag="ACFM8U_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC6333859.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTTKEEDLMAI"
     gene            complement(18404..19570)
                     /locus_tag="ACFM8U_000575"
     CDS             complement(18404..19570)
                     /locus_tag="ACFM8U_000575"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC6333860.1"
                     /translation="MLFHISASDGTTDMNTSQRNRLVSRWLNSYERYRYRRLIHAVRL
                     GGAVLFATATARLLHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGL
                     GVLWLNQHYFHGNLLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWF
                     DSGLMRAMNVLIGAAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTR
                     ERLEQNMVKMRQINARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTT
                     AAKLQSPKLNGSEIRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAE
                     HLHYQWQGFLWLSTNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(19603..19678)
                     /locus_tag="ACFM8U_000576"
     tRNA            complement(19603..19678)
                     /locus_tag="ACFM8U_000576"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19643..19645),aa:Thr,seq:cgt)
BASE COUNT         4056 a         5062 c         5758 g         4880 t
ORIGIN      
        1 aggattaagg ggatatttgg gtaaaattag gaggtatttg gtacgaaaac agccgaaatc
       61 ctgtgtttgg gtttcggctg tcgggaggga caggaatttt gcaaaggtct caggccgtct
      121 gaaaaacatt tttcagacgg ccttgttgat tcaatcaaat cagtctttca acttcgacaa
      181 ctgagtttgc acttttgcca ttttgtcttc caattccgcc aaatcggctt tgtctttttc
      241 caccaaatgc gctggggctt tttcggtgta gccgggtttg gagagtttgg cgttgagttt
      301 gtccaaggct ttttgcagct tctcggcttc tttgctcaaa cgggcggttt cggcagcttt
      361 gtcgatttcg actttcaaca tcaggcgcgc gccgttgcag acggcgacgg gcgcgtcttc
      421 gctttcgggt agggcggcga cttgctgtgc ttcggtcagg cgggtcatca tcggcaggta
      481 tttgaggtag tccgccaagt cgtctgcgct ttcgacaaac agcggggctt tcacgttggg
      541 ctggatgccc atttcgccgc gcaggttgcg gactgcgccg atcaaatcct gcaacacggt
      601 catttgcccg aatgccgtct gaacaatctc gccgctgtcg gcttcgggga agcgggcgag
      661 catgatgctg tcggcggttt tcgcgtcgca cataggagcg acggtttgcc acagttcttc
      721 ggtgatgaac gggataatcg ggtgcagcag gcgcagggcg gcttcgagta cgcgcaataa
      781 ggtatggcgt gtggcgcgtt ggcggctggc gcagccggtt tgaagctgca ctttggcaag
      841 ttctaaatac cagtcgcaat agtcgttcca catgaagctg tacagggttt ccgctgccaa
      901 atcaaagcgg taggtttcgt aggcttgcgt tacttgctcg atggtctgat tcagacggtc
      961 tacaatccac atatcgggga aggaatagcc gcgcggctca gtggcggttg cgccgtagcc
     1021 gcagtcttgg ttttcggtgt tcatcaagac gaagttggtg gcgttccaga ttttgttgca
     1081 gaagttgcgg tagccttcgg cgcgtttgaa gtcgaagttc acgctgcgcc ccaagctggc
     1141 gtagctcgcc atggtgaagc gcaaagcgtc cgcgcccata ctcggaatgc cttcggggaa
     1201 gagttttctc gaggcttctt ccactttcgg cgcggtttcg ggtttgcgca gaccggtggt
     1261 acgtttcacc agcagttttt ccaagtcgat gccgtcgatc aaatccacag ggtcaatgac
     1321 gttgccttcg gatttggaca tttttttgcc ttcgtggtcg cgcacgatgc cgtggatgta
     1381 cacgtcttta aacggtactt tgccggtaaa gtgggtggtc atcataatca tgcgcgccac
     1441 ccagaagaag atgatttcgt agccggtgac caagacgttg gacggcagga aggctttgag
     1501 ttcgtcggtt tcagacggcc agccgagcgt ggagaacggc acgagtgcgg aggagaacca
     1561 tgtgtccaat acgtcttctt ctcgggtcaa gcctgttttg ccggcttgtt tttcggcttc
     1621 cgcctgattg cgggcgacgt aaacattgcc tgcttcgtca taccacgcgg ggatttgatg
     1681 cccccaccac agttggcgcg agatgcacca gtcttggatg ttgttcatcc attggttgta
     1741 agtgttgacc cagttttcag ggatgaagcg taccgcgccg ctgtcgacgg cttttttggc
     1801 tttgtcggcg aggcttaaac ctttgaattc gctgtcaggt tcgccgccgt ttggcgtggc
     1861 ggacatggcg acaaaccatt ggctggtcag catcggctca atcactgagc ctgttcggtc
     1921 gcctttcggc gtcatcagcg tgtgcgcttt gatttcgacc aacaagcctt gttcctgcaa
     1981 atcggcaacc atttgtttgc gcgcggcaaa gcggtccaag cctgcgtatt tttcaggcag
     2041 ggcaaagcct ggttgcgctt cgcctttgaa gttgaacact tcggcgtttg ccagcacttt
     2101 ggcttccaag tcgaacacat taatcaggcg cgtgtcgtgg cgtttgccga cttcgtagtc
     2161 gttgaagtcg tgcgcgggcg tgatttttac gcagcctgtg ccgaagtctt tttcgacgta
     2221 ttcgtcggca atcacgggaa tggtgcggcc ggtcagcggc aggattaatt ccttgccaat
     2281 caaatgggta taacgttcgt cttcgggatt gacggcaacg gcaacgtcgc ccagcagcgt
     2341 ttcggggcgg gtggtcgcca caatcacggc ttcggcagga ttgtccgcca gcggatagcg
     2401 gatgtgccac atagagcctt gttcttccac gctttccact tccaaatccg atactgccgt
     2461 acccaaaacc ggatcccagt tcaccaagcg tttgccacgg taaatcaaac cttgctcaaa
     2521 caggcgcacg aacacttcgg ttacggtttc ggcgcgcacg tcgtccatcg tgaaatactc
     2581 gcgcgtccag tcggcagagc agcccacgcg gcgcatttgt tgggtaatcg tgccaccgga
     2641 aacttctttc cattcccaca ctttttccaa gaatttctcg cggcctaagt catgacggga
     2701 cacgttttgc gcggcaagct gacgctcgac cacgatttgc gtggcgatac ccgcgtggtc
     2761 ggtgccggga atccaggcag tgttgcagcc tttcatgcgg tagtagcggg tcaggccgtc
     2821 catgatggtt tggttgaagg catggcccat gtgcagcgtg ccggttacgt tgggcggcgg
     2881 cagttggatg gagaaagacg gtttcgtcaa atccatatca ggctggaaat agccctgctc
     2941 ttcccagttt tgataatgtt ttgattcgat ttcggctgga ttgtatttgt ctaacatgat
     3001 ggaactttgt gaaattaagg ttatttttga tgtgcggatt ataacgcaaa aaggccgtct
     3061 gaatcatttc agacggcctt tgacatacgg gttttaaaaa cggaacaata ccaggctgac
     3121 ggcaatcacc gccatacccg ttgtcaggcc gtaaacggtt tcgtggccgt ctgaatagcg
     3181 tttggcggcc ggcagcagtt cgtccaacgc caaaaacacc atcacaccgg ctatcacgcc
     3241 gaataccgaa ccaaacacgg caggcgacaa aaacggctgc aaaaccaaat agcccaaagc
     3301 cgctcccaaa ggttcggcca agccggatag tagacacgcc cataccgttt tcttacggct
     3361 gcgggtggca aaataaaccg gcgcggcgat ggaaatgccc tccggaatat tatggatggc
     3421 aatcgccaag gccaaaggca tcccgaccgc cggattttcc aaggtggcaa aaaatgtcgc
     3481 caagccttcg ggaaagttgt gcgccgtaat cgcaaacgcc gccatcatgc cgactcgcgc
     3541 gatatggcgg cgtttgcttt cttgaaacga cggatcttgc gcgtccaagg tttcatgcgg
     3601 gttcggcacc aggcggtcaa tcagcgcaat gccgcccata ccggccaaaa atgccatggt
     3661 cgccgccgca aacgcgtggt ctttatcata aatttcagcg aacgcctcgc tggatttact
     3721 gaaaatctcc gtcagagaaa catacaccat cgcaccgccg gcaaatgcca aaccaaacga
     3781 caacacgcgc ggattaggcg ttttggaaaa cattaccaaa ccgctgccta atacggtaaa
     3841 caaaccggca gccaacgtga tggaaaaggc aacggccaaa ttggacatcg aaaaatcggg
     3901 catgagaaaa cctgcgctaa aagctgggag aggttcagac taacactttt taatgcgcat
     3961 gataatagtt attatttatt ttattgattg gatacacgga ttttgaaaca aaaggccgtc
     4021 tgaaaaataa ttttcagacg gcctttaaat ttgaaatgtc actaaacctt atcgctttcc
     4081 agcttaagcc tgataacgcg acaggctcaa atcgtcgctg cggatttcgg tgtctttgcc
     4141 gctcacgata tcggcggtta attttgccga acccagcgac atggtccagc ccaaagtacc
     4201 gtggccggta ttcagaaaca ggttgtcaaa gcgggtgcga ccgattaacg gcgtgctgtc
     4261 gggcgtcatc ggcctgaggc cgctccagaa cgatgcttgg ctcaaatcgc cgccttccgg
     4321 gaacaagtcg ttgacgacca aagccaaggt ttcgcggcgt ttttcgggca gtttgatttc
     4381 gtagcccgac aattccgcca taccgccgac gcggattctg ttgtcaaagc gcgtgatggc
     4441 gactttgtag ctttcatcta aaacggtgga caccggcgcg ccgtctgaat tggtgaccgg
     4501 cagggtcaag gaatagcctt tgacgggata aatcggcaga tcgaggtcta actgcgccaa
     4561 cacagtccta ctgaagcagc cgagcgcaca gacaacggca tctgcttcaa accgccctgt
     4621 ttcggtttca acggctttga tgcgcagccc gttatggtcg atgcggctga tggtttggtt
     4681 gaaatgaaac tgtacgccct tttcctgaca caatttgtat aggttttcag tgaagaggtg
     4741 gcagtcgccg gtcgcatctg caggcaggtg caggccgccg gcaattttgg cggtaacgcg
     4801 tgccagcgca ggctcaaatt ctgcacattc ttcgggtttc agacggcggt acggcacgcc
     4861 gtagcgttcc aaaacggcaa tgtcttgttc tgccgcttcg acttctttgg tttggcggaa
     4921 aatctgcaac gtcccttttt tgcgttcctc aaaattcatg ccggtttgcg cttcaaaacg
     4981 gcggaacatt tcacggctat attcggaaat cctgaccatg cgctctttat tggtttgata
     5041 gtgcgctgcc gtgcagtttt gcagcatttg ccacagccat tcgatttgat acaggctgcc
     5101 gtcggggcga aacagcaaag gcggatggct tttaaacagc catttcagcg ctttggtcgg
     5161 gataccgggt gcagcccaag gcgtggtata gccgtaagaa agctggcctg cgttggcaaa
     5221 actggtttcc atcgccacac cctcggcgcg gtcgatgacc gttacttcat gtcctgcctc
     5281 tgccagatac cacgcggaag acacgccggc aacacccgca cctaaaacaa gcactttcat
     5341 gtttctccct ccggcttttt caaaacagac ttaatatgcc gtgccgtctg aatattcgga
     5401 ttcagacggc ctcggatatt aatgcggcaa ttcgccgttt gtgatttttt gtttgaagtc
     5461 gcgcgtttca ttgacgatga ctttcgccat caataaaagt gcaatcaggt tgggcaatgc
     5521 catcaagccg ttgaatgtgt ccgaagccag ccacaccaaa tcaaggctca acacggtacc
     5581 cagcataacg gaagaaacat aaccgacgcg gtacaaaccg gcaaatttct cgccgaaaac
     5641 atacaccgcg catttttcgc cgtaatagca ccagccgaga atggttgagt aggcaaagaa
     5701 aatcaggccg atggtgacaa tccagccgcc gatgccgggc agcatttttt ggaatgtgac
     5761 ggttgtcagt gccgcgccgc tcacttcagg tttgacaaac tcgccgcccg cgccgagcag
     5821 tcccattacc aacacgatgc cggtaatcga gcaaacgacg atggtatcca aaaacgtacc
     5881 ggtcatagaa accaaggcct gacggacggg atggtcggtt ttcgcggctg cggcggcaat
     5941 aggcgcagaa cccatacccg cctcattgga gaacacgccg cgcgccacgc cgtagcggat
     6001 gaccgtaccg atagcgccgc ccgccactgc ctgcgcgctg aacgcatcgg agaaaatcag
     6061 cttgacggca ggcatcagtg catcggaatt aatcgcgata atggaaagac cgcccaacac
     6121 ataaaacacc gccatagcag gcacgatgaa agaagcggct ttggcgatgc ctttaatacc
     6181 acctaaaacg acaacggcag tcagaacggt caacgtaatg ccggtatagg caggttcgat
     6241 accgaagctg gtttgcaccg cctgtgcaac cgagttggac tgcaccgagc tgccgatacc
     6301 gaaggaagcg aatgtgccga acagcgcaaa tgcaacggcc atccatttcc agtttttgcc
     6361 caagcctttt tcgatgtaat acatcgggcc gccggacatt tcgcctttgg aattgttgac
     6421 gcggtatttc accgccaaca cgccttcgcc gtatttggtg gccatgccga aaatggcggt
     6481 catccacatc caaaataccg cgcccgggcc gccggttacc accgcagtcg ccacgccggc
     6541 gatgttaccc gtaccgatgg tggcggacag cgcggtcatc aacgccgcga aatgggaaat
     6601 atcgccttcg tggccttcgc cgccttcata cttctttgac ggcacaaacg cctgtttcag
     6661 cgcatagccc aacatagtga actgcaagcc tttcagcata acggtcagca caatgccggt
     6721 gccgaccagc agcatcaaca taacagatcc ccagacccag ccgctgacgg tttcgaaaaa
     6781 ggctttgaga ttgtctaaaa acaattgcat ggctttctcc tttgtctgtt ttatttttaa
     6841 aacaccactt ttgtagtgtc ctgtaatttc agcacagaat attcaataag acaatatgtt
     6901 cttttgaaaa atacttttgg ttttttctcc gaaagcaggt cggttcaagc tgcggaaatt
     6961 gtttgcaatt ctttaaaagc agcggcggag gtcacaatga aatgtccgaa tggggatgtg
     7021 gcgggcggca gaaatcatca atgctgccga ctgccatact tctgaaatct acaaaatgat
     7081 gcatcgatca aacaatatac cgctttaaaa aaaccgatgc cgtctgaaac gctttcgggg
     7141 tttcagacgg catcaaaagg gtacggtcag cggatgatgc cgcgcgccga ttgtgcgaaa
     7201 aagtctcgga atacggcaag ctcggcttgg gtttcggcgc ggcggagaat gtctgccttg
     7261 gcttcttcaa acggaatgcc gcgatggtag agggttttgt acacgtcttt gacggcggaa
     7321 atctgctctg cggtaaaacc gttgcggcgc atgccttcgc tgttgagccc cgccggttcg
     7381 gcgcggtagc ccgatgccat aaagtagggc ggtacgtctt tgtgtacgcc tgcggcgaag
     7441 gcggtcatag cgtagtcgcc gatgtggcag aattggaaaa ccagcgtgta gccgcccaaa
     7501 acgacgtagt cgccgatggt aacgtgtccg gcaagcgagg cgttgttggc gaagatggtg
     7561 tggttgccga ccacgcagtc gtgcgcgagg tggcagtacg ccataatcca gttgtcgtcg
     7621 ccgatacggg tttcgccgat gccggttacc gtgcctaaat taaaggtggt gaattcgcgg
     7681 atggtgttgc cgttgccgat aatcagcttg gtcggctcgt cgcggtattt tttgtcctgc
     7741 gggatttcgc cgaggctggc aaattggaaa atgcggttgt tttcgccgat gctggtgtgg
     7801 ccgttgatga cggcgtgcgg gccgatttcg gtattcgcgc cgatttggac gttggggccg
     7861 atgacggtgt acgcgccgac tttgacgctg gagtcgagtt cggctttggg gtcgatgacg
     7921 gcggtcgggt ggatgagggt catgttttga atactccgct gcttgttaag acaaataata
     7981 gaaaatcaca gtttgtcgat ttcatcgcag accgcaatca cttgatcgct aaaatggcta
     8041 aatactgaag cagtatctac atccatgtaa ttaagcagtt caaaaataga ttggtgaata
     8101 tttctagcgt agttggtatc ttttattgca cctttaaaac aaatcggttc gtgatgtgcc
     8161 aaacgattac ggaaattgtt aatattggaa agttctcgaa aaatccattt ttggttatat
     8221 tgcacacttg gtgtagattt gggctttttt ggaaaaactt tcatcaatac tttccctgta
     8281 gcatcaaatt gggcatcttt ccctcctgca aacagatacc gccaaaaacc gaatcctagt
     8341 cctgcaacca attggttgtg gtcaaacttt cccccgctat tattttgtat tttagataat
     8401 gcaaccttga ttaattcagc agattctttc gtgccatttc ttaaaaagca accttgatat
     8461 tttaatgcgg gattcgtctg tggctgtatg ctgttatata accagtttct gtctttaaac
     8521 gcctgctgaa ggcaaatgtc aattttgttt cttaaaacaa tctcaaatat tgaaacaaca
     8581 tgaaaaagtt ctcttgataa agataaattc aataaataca attcagctgc tttgtcttcc
     8641 ccaacggcat ttttataccg ttccattctg aaatcagaaa aaatactaca gcattcctta
     8701 taatccacgc taacacctga ttctttattt ttaaattgac gtttcaaact atatttgata
     8761 cagttaggta aaactaatcc ccaccgtgtc cctgggtttg aataaaccta aaccggtggg
     8821 ttttcttttt attaggtcgt ctgaaacttt cagacgacct ttttccgaac gctcaaacca
     8881 cgcgtttggc gcacataatc acggcttcaa cggcgacttg cccatctact ttggcaacgg
     8941 cgttgaattt gccgatgccg cgccggctgg tcagcagctc gacttcaaag acgagttggt
     9001 cgccggggat gacttggcgt ttgaaacggg cttcgtctat accggcgaag aagaagaatt
     9061 cgttttcctt gcggccgcct tcgctgagga tggcgagtgt gccgcaggct tgcgccatcg
     9121 cttcgatgat gagtacgccg ggcatcacgg gcaggtcggg gaaatggcct tggaactggg
     9181 gttcgtttat ggtgacgttt ttgatggcgg tcagggtttt catggactcg aaggcggtga
     9241 tgcggtcgag ctggagaaac ggatagcggt gggggatgag tttttggatg tctttggctt
     9301 cgatggggag ttgtacgtcc atgtctgtcg tattccttga ataaaatcgg tttggattta
     9361 ttgcgtgtct ttgccgtctg aaagctgctg ttccagcgtt ttgaggcgtt tgttcatttc
     9421 gcttaagcgg tggatatgga cggcgttgcg cgcccattct ttatgggtgg acatcgggaa
     9481 gatgccggcg aggtgtttgc cgctttcggt aatgctgtgg gtgacggacg tgccgccgcc
     9541 gatggtggtt ttgtcggcga tttcgatgtg tccgaccgtg ccgacaccgc cgccgatgat
     9601 gcagtagctg cctatggtta cgctgcctga gatgccggtt ttggcggcga tgacggtgtg
     9661 cgaaccgatt ttgcagttgt gtccgatttg gacttggttg tcgattttgg tgccgttgcc
     9721 gacggtggtg tcgctcatcg cgccccggtc gatgttggtg ttcgagccga tttccacgtc
     9781 gtcgcccagc gttaccgcgc cggtttgcgg gattttaaac cacgaatcgc cggcgaaggc
     9841 gagtccgaaa ccgtccgcgc cgatgactgc gccgctgtgg atttcgacgc gtctgcccag
     9901 tgtacagccg taataaacga cggcgttggg gtgcatgacg acttcgtcgc ccagtttgca
     9961 atcgtgttgg acgacggcgt ttgccaagat gcggcagcct tcgccgagca cggtgtttgc
    10021 gccgatgtag gcgttcgcgc cgatttcgca gcttgcggga acggtggcac tcggttcgac
    10081 gacggcggtc ggatggatgc cgccgtgcgc tttgacgacg ggtgaaaaca ggcgggcgac
    10141 tttggcgaaa tagagatagg ggtcgtcggc gacaatcagg ttgcgccctt caaatccgtc
    10201 tgccgctttg gcggaaacga tgaccgcgcc cgcgctgctg tcgtggactt cggctttgta
    10261 tttcggattg gcaaggaagc tgatgtgttc cgcctgcgcg tctgcgagcg ggcgcacggc
    10321 ggtaacggaa atgtcctcgc cgcgccattc gccgccgagc cgcgcggtga tttgggacag
    10381 ggtgtaggtg gccggaatca tggttttcct gttcggtatg ccgtctgaaa gggtcagcgg
    10441 gcgttcattt ctttaatgac gctgtcggta acgtcgtatt gggtgttgac gtaaatcacg
    10501 ttctgcaaaa tgacatcgta accttcctgt ttggcgattt tgacgatgac gcggttggcg
    10561 ttttgctgga gggaggcaaa ctcttcgttg cggcggaggt tgtagtcttc ttcaaactgc
    10621 gcctgttttt tgcggaacgc tgcgaccagc ccgcgccatt tttcttcggc ttgcaccttt
    10681 tttgcgtttc tgagtttgcc ttcggcaagc tgcctttcca aatccagacc ttcgcgttgc
    10741 agtttttgca attcgtcctg acgagcggaa aattcgctgt ccagcgtttt ttgaatcttg
    10801 cgcgcctgct tggattcgag gtagatgcgc tcggtgttga taaagccgat tttttggaag
    10861 gtgtcggcgt gcgcgcctgc ggtgcagcac aaaccgatca gagccgcggc aaacgcgcgg
    10921 gtcaaacggg tcatggtaaa actccttcga atgttgccgc gaaatgccgt ctgaagggct
    10981 tcagacggca tttgcgggat tagaacgtcg tgccgagttg gaattggaag cgttggattt
    11041 cgtcttccgg ttttttcttc agcgggtagg cgtagctgaa tttcatcggg cctaaaggcg
    11101 agagccaggt aaccgcgccg ccggcggaat agcgcaattc gttggtaaag gtggatttat
    11161 gggtattgcc ggcgccgtaa atgttttgaa ccctgccgcc ggtcgcggaa ctgctgttgt
    11221 cgtcgtaggt tttgccgtcc cacacgctgc ctgcgtcggc aaacaggctc aggcggacgg
    11281 tgcgcgcgtc tttcgcgcca ggcatcggga agagcagctc ggcggagacg ttggcttttt
    11341 tgttgccgcc gtagctgatt ttttcgccgt attcgtcata cactttcgga ccgagtgtgc
    11401 cgctttcgta tccgcgcacc gaacccaggc cgccgccgta gaagttttca aagaagggga
    11461 tttctttggt tttgccgtag ccgcccgcaa tgccgacttc gccgcccagc atcagtgtga
    11521 aggttttgct taaggggaag aaccaggttt ggttgtgggt ggcggagtag tattgcagtt
    11581 tgctgccggg cagggcgatt tcggcgttca cgcccgtcag gtagccgcgc gtcggccata
    11641 acgcgctgtc ggttttgttg cgcccccagc cgacggtacc tttgtacagc cagcctttga
    11701 agctgccgtc tgtgccgtcg gttttgccgt atttcttgat aaagtcggca tagtgtttgg
    11761 gcgctttgtt gtaggtgttg acggtcaggt gttctgccac caaaccgaaa ttcacgcggt
    11821 cgtattcggt aacaggcacg ctcatgcgga tgcctgcgcc tgccgtggtg gttttatatt
    11881 gtttgatgct ggtcgatgct ttgcgcgggt cgaaggcttt tccgtaaaca tcgtagccta
    11941 ggctgacccc gtctgccgtg aagtacgggt cggtaaacga cagcgagccg ttgagcgtgg
    12001 ttttgcttcg tgaggcgcgc agggcggccg acttgcccgt accgaacagg ttgtcttggg
    12061 aaacgcctgc ggacatgacc aggccggtat cctgtaccca gcccgcgctc aagtcgagcg
    12121 agccggtgga acgttcggtc aggctcatgt tcaaatcgac tttgtcgggt gtgccggcaa
    12181 gcgggacggc gtcaaactgg acattgtcga agtagcctaa aagctcgacg cgctctttgg
    12241 agcgttgcag cttggaagtg tcgtaaggcg cggattccat ttggcgcaat tcgcggcgca
    12301 cgacttcgtc gcgggttttg ttgttgccgg tgatgtggat ttcgttgacg tagattttcc
    12361 ggcccggttc gatgtgcagg acgaaatcga cggttttggt ttcggcgttc ggcagcggct
    12421 gtacgctgat ttcgctgtat gcgtagcctg ccgagcccat gcggttctga atctcaccca
    12481 aaacggcggt catctgctgg cgttcgtacc atttgccggg cttcatggtc agcagctttt
    12541 ccagttcggc tttggggact tcgttggtgt cgccttcgat ggagactttg ccccaacgga
    12601 aacgtccgcc ttcgtggacg gtgattttga tggtctgctt ggttttgtct tcgttggttt
    12661 ggatgtcggt atcgaggatg cggaaatcga agtagccgtt gttctggtag aagtcggtta
    12721 ctttttccat gtcttgggca aatttctgct cgttgaattg gttgcttcgt gtcagccatg
    12781 tccaaatgcc gccttcggtc agcgacatct gccgcatcag tttgcggtcg gaatagactt
    12841 ggttgccttc aaattcgatg tcggtgattt tggcggattt gccctcgtca atcgtgatgt
    12901 cgatgtcgac gcggttgcgg gcgagtttgg ttactttggg cgtgatttgg atattgagtt
    12961 tgccgcgccc gaggtattct tctttcaggc cggcgactgc ctgattgagt gtcgcctgat
    13021 taaagtattg cgactgcgcc agcccgaacg attcgaggtt tttcttaatg gcgtcgtttt
    13081 gcagcatttt tgcgccggtg atgttgagcg agccgatggt ggggcgttcg ataacggtca
    13141 gcaggagctg cccgtccgca gtctcgacgc gtacgtcgtc aaagaaaccg gtggcgtaca
    13201 ggcttttgat gatggcactg ccgtgtgtgt cgttgtaggt gtcgccgact ttgacgggca
    13261 ggtagttgaa tacggtgctc ggctcggtac gctgcaagcc ttcgacgcgg atgtcttgga
    13321 tggtgaagtc ggcaagtgcc aaaggcgata tgcccaagac catcagtgcg gaagcaatct
    13381 gtttcagttt cattgtcagt tccttgtggt gcggaatgcg gtttcagacg gcattccgaa
    13441 acgtaaaatc taaccgagca gccgggtaac gtcgttgaag aaggcgaccg ccatcatcag
    13501 catcatcagg gcgagcccga agcgcaaacc gatgttttgg acgcgttcgc ccaaaggttt
    13561 gccgcgtatc cattcggcag tataaaacac gaggtggccg ccgtccaaaa cggggacggg
    13621 cagcaggttc agcacgccga ggctgatgct gaccagtgcc aaaaattcca aataactttg
    13681 caagccgagt tcggcggact gtccggcaat atcggcaatg gtcagcggac cggaaatatg
    13741 gctgacggag gcgttgccgc tgattagttt gccgaaaaat ttgagggttg tccacgagtg
    13801 ggaaacggtt ttttcccagc ccatgccgaa tgcgcggaca acagacggac ggtagctgcg
    13861 gcggatttgc gcgtcccacg ccctgtccgg ctgcggacgg aggccgacgc gcccgattag
    13921 ggtgtggtcg ggctgttcga cagtatcggg gcggatgtcg gcggtatggg tttgtccggc
    13981 gcgttcgtag gttaaggcaa ttttcctgcc cgggctttgg cgggtcaggt ttgcccattc
    14041 ttgccatgag gcgatgggtt tgccgtcggc ggcagtcagc ctgtcgcccg gtttcaggcc
    14101 tgctttttcg gcggggctgc ctttttccac gccgccggca acggttgtga ttttaaaggg
    14161 catcagtccg atgtagcctt ggtttttggc gattttaccg gcttctggcg tgcctgcggc
    14221 atcgatggtg cggacggttt gcgcgcccga tgccgtctga acgccgacgg cgactttgcc
    14281 ggcttcgagg ttgaggacga tttcggtttg cgcgctgccc caatctgcaa cgggtgtgcc
    14341 gttgacggat tgtattttgt cgccgctttg gaagccggca cgggcggcaa tggtgtcggg
    14401 ttcgactgtg ccgacgtagg ggcgcagttc ggttacgccg aaggaaaagc tcagtccgta
    14461 cagcaaaacc gccagtgcga ggttggtcag tgggccggcg gcgacgatgg cgatgcgctt
    14521 ggcggggtgt tgtttgtcaa aagcgtaggg taaatcggct tctgatactt cgccttcgcg
    14581 cgtgtcgacc attttgacgt aaccgcccaa cggaatcggg gcgaggcacc attcggtgtc
    14641 gccgcgcttt cgggtgaaaa acggtttgcc gaagccgacg gaaaagcgta cgactttgac
    14701 gccgcacaat ctggcaacga tgtagtgtcc gaactcgtgc aggctgacca aaatcaggat
    14761 ggcgaagata aaagctagaa gggtgtgcaa atggttttcc tttgataacg gtgttcagac
    14821 ggcatcagcg cagtgtgccg ataaatgctc gcgcttgtgc gcgtgtccgg gcatcttgcg
    14881 ccaacagccc ttctatatcg cctatgccgt ctgaaaagtc ttgtgcgaga caatgggcga
    14941 cggttttggc aatgtcggta aacttaatct gtccgtccaa aaaggcggcg acggcggctt
    15001 cgttggcggc gttcaatacg cagggcgcgg ctccgcctgc gttcatggct tcataggcga
    15061 gcttcaggca ggggaagcgg tcaaagtcgg gcttttggaa ggtcagcgcg gacaatgcgt
    15121 cgaaatccag gtcgccgaca cccgaatcga tgcgctcggg caaacccaaa caataagcaa
    15181 tgggcgtacg catatcggga ttgcccagtt gcgccaacac ggagccgtcg cggtagcgca
    15241 ccatgctgtg tatcacggat tgcggatgga tgacgacttc gagcttgtca ggcggacagt
    15301 tgaacagcca atgcgcttca atcagctcca aacctttgtt catcatggtg gcggaatcga
    15361 cggagatttt gcgccccatg ctccaattgg ggtgtttgac cgcttgggcg ggcgtaatgc
    15421 tgtcgaacgt gtttaaatcg gcggtcagaa acgggccgcc ggaagcggtc aggataatcg
    15481 aagcgatgcc gtgttcgttc agacggccgg cgtaatcgcg cggcaaaact tggaaaacgg
    15541 cgttgtgttc gctgtcgacg ggcagcactg ccgcgccgtt tgcacgggcg gtttccataa
    15601 acaacgcgcc ggaaaccacc agcgtctctt tgttcgccag ataaatggtt ttgccttttt
    15661 gcgccgctgc gagcgcggaa ggcagcccca ctgccccgac gatggcgcac atgacaccgc
    15721 tgacttcgtc ggcagacgca acgtcaacca atgcctgcgc gccgtgtaaa acctgcgtcg
    15781 ccgtgccgtc gcgtttcaac agggcttcaa gccgggcggc gtgttccgca tcggcaacga
    15841 cggcatattc ggggcggaac gtttgacatt gagccgccaa tttctcgacc tgcttatgcc
    15901 ctgccagcgc gaatacgcgg aatttttcgg ggtggcggga gacaacgtcc agcgtgcttt
    15961 cgcctatgct gccggtactg cctaatatgg tcaggacttg tggtgtcata atgaggataa
    16021 ctttataccg gatgccgtct gaagcgtttt cagacggcat agaatcaatt taaaaccgac
    16081 atcatcgccg catagacgct gataacggca atcaggctgt cggtacggtc gaacacgccg
    16141 ccgtgtccgg acagcaggtt gctgctgtct ttaatgcccg ccgcgcgctt gagccagctt
    16201 tccaacaggt cgccgcatac gctgacaacg gtcagcacca aaccgattaa cacggtatcg
    16261 aaccagcctg tatcgaatgc cagccagccg gcacttcgta cggcggtcat gtacactgcc
    16321 acgcaaaccg cgccgccgat tgcaccttcc cagcttttgc cggggctgat tgccggtgcg
    16381 attttgtgtt tgccgaacgc cttgccgctg aaatacgcgc aaatatcggc aacccacacc
    16441 aaacccatca cggcgagcag cggcagggca tcatcgggat gcgggcgcag ggatacgagc
    16501 gcgaaccaaa acggcatgac cagaagccag ccgacggcat aaacctgcca accgccgttg
    16561 agcctccatt tgaatctcaa ccataaaggc ataacggcga gccaaaatgc caaaacaaca
    16621 taccaaacca aattaggcag catccagccg cccgcatagg caaccacgcc gaaaaccaag
    16681 gttgcggcga ggtaatggtt ggttttaatt ttgcacaaac cgcccatacg ggcatattcc
    16741 cacaaggcaa tcagggcaat cagtccgcaa aatgcagccc acaaccattg cggcgcgtaa
    16801 aacagcatgc ccagcatcag cggcagcagc cacatggcgg ttattacccg ttgtttcagc
    16861 atattcagtt cctttgctgt tcgataggca gttgctcgga ggtgcgtccg aaccgccgtt
    16921 cgcgtttttg gaacgaagcg acggcatcgt ccaaagcctt gccgtcaaaa tcgggccaca
    16981 aaatatcggt gaaatacagt tcggcataag ccatttgcca gagcaggaaa ttgctgatgc
    17041 gcgtttcgcc gccggtgcgg atgaacaaat ccggctcggg cgcatcgccc agcatcaagt
    17101 gtttcgccag cgtgtcttcc gtaatctcgg atacgccttt ggcaatcagt ttgtttgccg
    17161 cctgcaaaat gtcccatctg ccgccgtaat cggcggcaat gctcagggtc agaccggtat
    17221 tgtttgccgt caacgcctcc gcctcttcga tgcccttcag gatttgacgg ctgaagcgtt
    17281 cgcggctgcc cagtattttc aggcgcatat tgttttcgtg caggcggcgt acctgttttt
    17341 gcaaagcctg taaaaacagc cccatcagga acgaaacttc gtcttcgggg cggcgccagt
    17401 tttcggttga aaaggcaaac acggtcagat attgcacacc cagtttggcg caatgcttca
    17461 ccatattttc caatgcgtcc aaaccgcgtt tgtgtcccat tatgcgcggg aggaaacgtt
    17521 ttttcgccca acggccgttg ccgtccataa tcacggcgat atgcttggga atggcggtgt
    17581 gttccaaaac ggcctgcgtg ctgcttttca tgtctgcctt tcgcggttcg gcattcaaat
    17641 gccgtctgaa cgccgaaccg tgcaggttaa attgccatca aatcttcttc tttggtagtc
    17701 aggagtttgt cggcttcggt aatgtatttg tcggtcagtt tttgaaccgc ttcttcgccg
    17761 cgacgtgcct cgtcttcgga aatttctttg tctttgagga gttttttgat gtggtcgttg
    17821 gcatcgcggc gcacgttgcg gatggagacg cggccttctt ccgcttcgcc gcgtacgact
    17881 ttaatcaggt ctttgcggcg ttcttcggtc agcatgggca tcggcacgcg gatcaggtcg
    17941 ccgacagctg ccgggttcag ccccaagttt gaatcgcgga tggctttctc gactttggca
    18001 gccatattgc cctcaaacgg tttcacgccg atggtgcgcg cgtccagaag cgttacgttg
    18061 gcaacttggc tgacggggac catgctgccc cagtattcga cttccacttg gtcgagcagg
    18121 ccggtatgcg cgcggccggt acgcactttc gccagatttt ctttcagtac ttcgaccgaa
    18181 cgctgcatct tgccttcggc tgttttttga atatcgttga tcatattgtt ctttcggtgg
    18241 gataaggtgg gcgggagacc gtctgaacgc gtttcaagcc gttcagacgg cataaagacc
    18301 gttaaccgcg aatagtaccg ttattcgggc ataacgacaa ggcaggcgga ttggagatgc
    18361 cgtctgaagc ggcaggcgtt tcagacggca tcgtgtccga ccgtcagccg tgttcccgtg
    18421 tttcaagcag gctttggcgc aggtgttggc gttcgtgggc atccagccat ttgcggcggg
    18481 tgcgttgcag caggatgacg agggcggaaa tttcctgacg catattggtg ctgagccaga
    18541 ggaagccctg ccattggtag tggaggtgtt cggcgagggc ttccagttcg gggttgatgg
    18601 cggtgtcgat gcggatgcgg cgggcgtgtc tgccgttgat aagggcgacg gtttgttgca
    18661 ggtcggtttg gagcagtgtg aagtggcggt cgagcagccg gatttcgctg ccgttgagtt
    18721 tgggagattg cagcttggcg gcagtggtca ggagcagctc ggtggtgttg acaattttac
    18781 ggtgggcgtg ctgcatggct tccatcatgg cggggctgat gcggctttcg cccgatgtgg
    18841 cggcgaggtg gctgcggctt ttgaccatgc gtgcgttgat ttggcgcatt ttaaccatat
    18901 tctgctccaa acgttcgcgc gtcatacgcc tgccgttgct gatttcggca atcattttgc
    18961 tgcagtcggt caggttgtcg gcaagcatga aacgccacat cagtgtggat ttcagcggca
    19021 gcagtttggc ggcggcgatg gcgatggccg cgccgatgag gacgttcatc gcgcgcatca
    19081 ggccgctgtc gaaccattcg ctgccgttgt cgccgatgag catgcacatc gtcagccccg
    19141 ccagcatagg gacgtagccg tttttgccga ccgccgccca gccggccagt gcgcttgccg
    19201 tgccgacggt gaggtagaag aggaggttgc cgtggaaata atgctggttc agccataaaa
    19261 cgcccaaacc cgcgcccagc ccgatgaccg tgccgagcat acgttccacc gccttggagt
    19321 aaatcgcccc ttgaaactgg agcatgccga ggacgacgaa gacggtcatc cctatccact
    19381 cgccgtgttg gaggtggagc agccgggcgg tggcggtggc gaacaggacg gctccgccga
    19441 gccgcacggc gtggatgagg cggcggtagc ggtaacgttc gtaggagttg agccagcggc
    19501 tgacgaggcg gttgcgttgc gaggtgttca tatcggttgt gccgtctgaa gcggaaatgt
    19561 gaaaaagcac aggcttcccg agggagggtc tgtgcttggt attggtgccg gagaagggaa
    19621 tcgaaccccc gaccttcgcg ttacgaatgc gctgctctac cgactgagct acaccggcgt
    19681 tttttcgtca tgatatatat gaacggttgt ttgtgcaact tttcgggcgg gcggcaaggc
    19741 agtgcgcggt atagtg
//
LOCUS       DAXHSJ010000022        19753 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000022 DAXHSJ010000000
VERSION     DAXHSJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.022"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(203..278)
                     /locus_tag="ACFM8U_000577"
     tRNA            complement(203..278)
                     /locus_tag="ACFM8U_000577"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(371..730)
                     /locus_tag="ACFM8U_000578"
     CDS             complement(371..730)
                     /locus_tag="ACFM8U_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC6333861.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(753..1397)
                     /locus_tag="ACFM8U_000579"
     CDS             complement(753..1397)
                     /locus_tag="ACFM8U_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFC6333862.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1399..1935)
                     /locus_tag="ACFM8U_000580"
     CDS             complement(1399..1935)
                     /locus_tag="ACFM8U_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HFC6333863.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPSIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1947..3044)
                     /locus_tag="ACFM8U_000581"
     CDS             complement(1947..3044)
                     /locus_tag="ACFM8U_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6333864.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3047..4483)
                     /locus_tag="ACFM8U_000582"
     CDS             complement(3047..4483)
                     /locus_tag="ACFM8U_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6333865.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="ACFM8U_000583"
     CDS             complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="ACFM8U_000583"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC6333866.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            complement(5473..6120)
                     /locus_tag="ACFM8U_000584"
     CDS             complement(5473..6120)
                     /locus_tag="ACFM8U_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC6333867.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKVSFEDYQQFPARPFDEIHQELTALFEMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="ACFM8U_000585"
     CDS             complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="ACFM8U_000585"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC6333868.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEAALAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAVCL"
     gene            complement(7294..7431)
                     /locus_tag="ACFM8U_000586"
     CDS             complement(7294..7431)
                     /locus_tag="ACFM8U_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HFC6333869.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFINRALLDCLMLGWFR
                     L"
     gene            7495..8571
                     /locus_tag="ACFM8U_000587"
     CDS             7495..8571
                     /locus_tag="ACFM8U_000587"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFC6333870.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCIRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8778..10244)
                     /locus_tag="ACFM8U_000588"
     CDS             complement(8778..10244)
                     /locus_tag="ACFM8U_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC6333871.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10442..11365)
                     /gene="speB"
                     /locus_tag="ACFM8U_000589"
     CDS             complement(10442..11365)
                     /gene="speB"
                     /locus_tag="ACFM8U_000589"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC6333872.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGIIDPAHSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11464..13356)
                     /gene="speA"
                     /locus_tag="ACFM8U_000590"
     CDS             complement(11464..13356)
                     /gene="speA"
                     /locus_tag="ACFM8U_000590"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC6333873.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQIVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13523..13807)
                     /locus_tag="ACFM8U_000591"
     CDS             complement(13523..13807)
                     /locus_tag="ACFM8U_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6333874.1"
                     /translation="MKKLLLAAVVSLSAAAAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="ACFM8U_000592"
     CDS             complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="ACFM8U_000592"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC6333875.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFQDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAQ
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            complement(15824..16525)
                     /locus_tag="ACFM8U_000593"
     CDS             complement(15824..16525)
                     /locus_tag="ACFM8U_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC6333876.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16668..17816)
                     /locus_tag="ACFM8U_000594"
     CDS             complement(16668..17816)
                     /locus_tag="ACFM8U_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC6333877.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17977..18240)
                     /gene="rpsT"
                     /locus_tag="ACFM8U_000595"
     CDS             complement(17977..18240)
                     /gene="rpsT"
                     /locus_tag="ACFM8U_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC6333878.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18543..19685
                     /locus_tag="ACFM8U_000596"
     CDS             18543..19685
                     /locus_tag="ACFM8U_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC6333879.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5076 a         5113 c         4593 g         4971 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta gttgtccagg
       61 acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt tattccatct
      121 caaaacaatc atctcaaaaa tgcgtttctg accgccggta aaacaaaacc ctctaagaaa
      181 atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg gacctgcgga
      241 ttaacagtcc gtcgctctac cgactgagct atcggggaat aagcgcgaat aataaaacag
      301 aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc aagcccgcaa
      361 tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca tccaatagga
      421 ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct ttacccgcct
      481 gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat tccccgcagg
      541 cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc gcccctaccc
      601 tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg gttgagataa
      661 cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata
      721 attgctgcat aacccctcct ctctcccaag ttttacacgc ggttgctgta acggcggtat
      781 aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc gccgataacc
      841 aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct ctgaccggaa
      901 ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat cagcagccgg
      961 tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc cgccgcctca
     1021 tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa cgccttaccg
     1081 ctgccggaca attgttccat cagcaacggc aattgctcgt ccgacaaatt gatgccgtct
     1141 gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta caacaggttc
     1201 aaagcggtat cgatgtcggc ggtgtccgcc caggtaagca gcttctcact gctgaccatt
     1261 tcgtccgcat ccgctttcaa caggctgtgc aacaaagttt taccggcact gggggcatcg
     1321 ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt tgcttgtagt
     1381 gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata acattgcgct
     1441 aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg gaacattaag
     1501 attatgtttt gcaagatatt tgtgatacac gtcgatactg ggctgagagc gtatatccat
     1561 cttggtaata ccgacaacga cgggggcctt ctccagcagc cctcgaaacg aatgtaaaaa
     1621 gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga ccaaacccat
     1681 actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg gcgtaccata
     1741 taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg ccactgtcgt
     1801 attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc
     1861 gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg tgaaaataat
     1921 tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat cagcctttgc
     1981 agaaggccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt tttcatcatt
     2041 tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc ggagtatgcc
     2101 tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt tgccgcaaga
     2161 taattcagaa tatcattgag gtttaaaggc atcactttat acaacacgtt aaggttgacg
     2221 gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacatttgc caaccgggtc
     2281 aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata aatcaacctg
     2341 ccctgcgccg tccaaatgga aaactgccac atacaggaca taatgcctac tttagccttt
     2401 tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg
     2461 cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat cgaggggaaa
     2521 acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt cttttccgca
     2581 aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt aaccgtacgc
     2641 tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga tttcccataa
     2701 acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt
     2761 ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc gacgggtata
     2821 tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc atcggtatcc
     2881 gccaaaacca aatcgggcac ggcactgccg tctgaagggg atactgtttc ataacgggtg
     2941 gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt catccccgcc
     3001 aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat tttccgtacc
     3061 gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc agcataacga
     3121 cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc cccaacctat
     3181 cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg gactcgtcgg
     3241 gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga ccatacatca
     3301 gcaacgactc tacttcctta accgcatcgt ctgccggaga cgaaccggtg ttaatcaacg
     3361 aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata tttttcggca
     3421 gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct ttttccaaat
     3481 catcgaaaac ttcatggtaa cccaagctgt acccccaccc cagcatccgc tctttaacct
     3541 gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca aaacggtcta
     3601 tgtccgcgtg ttggtaatcg agtttcaacg catcgataat caggtttgca ggtttttcgg
     3661 aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact ttgtctttaa
     3721 ggattttcgc accttgatcg gcgcgaacca aaccggcaat cgcaccgatt tcctcttgac
     3781 tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt tcttcggctt
     3841 cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc
     3901 cttccgcacg tttttcaatc tcctgagtac cccatcccaa accttcttct gccaagacgc
     3961 ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt gccaaaagtt
     4021 cggaaggaat cagtttgccg tatttttgca gattgtctgc caaaacatcg acatccccca
     4081 cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct tcaccatccg
     4141 gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa ccgaattgct
     4201 tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg gcggatgcgg
     4261 tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa
     4321 tctgatcgac ccagtccgaa tcattgcttc cgggtgtctg cccctgcgta ttgccgacac
     4381 cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc aaaagcatca
     4441 gcaataccgc cagtgccaaa ccgataatta atgagttttc caaaggatac tcccgatacc
     4501 gtaacgagcg gataactgac cgcctgcaac gaccattgtc cgcttttatt attaacgatt
     4561 tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa caatatgcct
     4621 tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc cgatataaaa
     4681 ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa
     4741 ataacggatg ataagtcagg cgcactttgc ccgacggcat accgaacgcc gacaagtaat
     4801 cgccaataaa tccattgaga ttttttcttg tccaattttt ttggttcgtg gctgttacgc
     4861 ctgtatattt aaggtgtttg agtacatcta acggcgtatc aaagtctagt attaccttaa
     4921 aatcctcaca ccataaaagc tcaaaatctt tggctaacca agcctgccat tgggataaag
     4981 tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg tctttgccaa
     5041 aggttgcaac cgccaataat ccgtttgctt tcaagcctgt tttgcaatgg gcgataaaag
     5101 cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca aattgtcgtt
     5161 gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag
     5221 ccagttgggg ctgcacatcg cacaaatcat taaataacca ataattcgct gaaatctgtt
     5281 tttgcagcaa ggcactcaac attcctgagc cgcagcccaa ttccaacacg ttttccaatg
     5341 gcatatccgg caaataatct tgcaaatgcg ccattaaatt aatcgtcatt ttttgttgga
     5401 ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga atgcgcgatt
     5461 tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta
     5521 atgtccgccg tcaatttccc gaacggtgca acgcggtgtc caatatcggt gctgattggc
     5581 aggcataaaa attttatcgc ctgatccgac caaggcattt gtccagcgaa tcaaatctgc
     5641 gcgcctatct tgcccgatca tctcaaaaag tgcggtaagt tcttgatgaa tttcgtcaaa
     5701 cgggcgtgcg ggaaattgtt ggtaatcttc aaaagatact ttatcgccac acattctgcg
     5761 ttcaaatttt aaacgggtgt tttccgtgag gttctccaat gtgcctttaa aaacggcgca
     5821 agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt
     5881 taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc acgccaccaa
     5941 acggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat aatcgtagca
     6001 aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg gcggcgttcc
     6061 ccaacctgca aaatacagga ttaaatgtcc gccttgatga ttgtaaaatt ttgttttcat
     6121 atcatatcct tacaggcaca ccgcaaactg ccgcacttca tccgttgtca tatctgccgt
     6181 taaagacagg cggattctgg atgtgttttt aggtactgtc ggcggtctga tgggcaggca
     6241 ataataaccc tgcctttgca gatattccgc tttggcaagg gcggcttcat tcccgcctaa
     6301 aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg ccacttcccg
     6361 ccgtaaaaat gcacttaact gctcaagatg gcttctttct tttgagaatt gcggcaatcg
     6421 ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg aaaaaatcaa
     6481 tgggcgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg catacgcccc
     6541 caccgaggct aaggctttac cgaaagtgcc gaccaataaa tcaatctcgg caatcaaatt
     6601 atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt gggcttcatc
     6661 cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt tcaaatccgc
     6721 cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt caaattttcc
     6781 gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat aacggaaaaa
     6841 cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tggacaaatt tatctgccaa
     6901 aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt gatagccgct
     6961 gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt ccaaatcggt
     7021 ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt
     7081 accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca aacccaaata
     7141 atcattagac gacatattca gcattttgcg gttttcccgc gtaatatacc gcccttgatg
     7201 aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa gctgttgttt
     7261 aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac ccagcatcaa
     7321 acaatcaagt aatgccctgt taataaaaac cgttttgccg ccgacctttg ccacacccct
     7381 gccttcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca ttacccaatc
     7441 cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc aggtatgatt
     7501 tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat ctgccgcact
     7561 gctgcactat tgataagcgg tttttcatac gcaaacaccg tcattcccga tgtctccccc
     7621 gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt cctctacacc
     7681 gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat
     7741 ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat ccccaaatcc
     7801 atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga caaccctttg
     7861 gggccggttt ttgtccgctt aggcgacccc aaactcggtt tgggcataca cgggaccaat
     7921 gcgccggcca gcgtccccgg ggttcggagt cacggctgca tccgcatgaa atcgcccgac
     7981 gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat ctatcaaatg
     8041 gcggggctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg cgacccttac
     8101 ggtaagaaca accttgacat cgcctctctg aaaaaaagta ttgcgcaatg ggcaaaaaca
     8161 cagggtaaaa ccatcgcgcc cgagaaagtc gatgctgtac tcaaagaccg caccggatcg
     8221 gccgtctgcc tgacctgcgg caaaaacggc aagatgaaga tgccgctcaa atcgctggcg
     8281 tggatacagg gttcttcctc atacagccaa gctgaagtca ttgaacaaac tgaggaaacc
     8341 aactctgccg aaatatccga gacacgcacg cccgaagtgc ctgatgtaca cacgcccgag
     8401 gcgcaaccgc atttaaatac ccaatccgac ggcacgccga ctgcctatac cgaaccggct
     8461 gccgattcat cgccgcaagt agaaacacct gatcaggctg cttccgagcc ggttgatgta
     8521 ttattttcaa tagatgtgat acggcaggga aatttgcgtt taggtaattg aataaccttc
     8581 tgattattta atgcttatgt ttatcagaag ttgataggcg gtttggtttt tgtttgactt
     8641 ttcctacacc gccgcctgcc gctttctgca acattcaagc gcacaaatat gccatctgaa
     8701 ggctttagac ggcatatttc acgataatag tcgcccaatc aaataaacac agattggaaa
     8761 tttattttat ttttccctta ccaatacccc agaactttcc accaaatgct gccgataacg
     8821 aaaaaaatca gaaaattgac tacgctcatg ataaaacccg ccttccacca ttctcccatt
     8881 gtggtgtagc ccgaaccgaa aatcacaggc gaagtaccgg tcgcataatg agtgagggtc
     8941 atcataatgt tggatgcggc cgccatcatc agcgcggtcg gcatcgccgg tgcattcagt
     9001 gaaacggcag cagcgagaaa tgcgccgaac atagcggtaa tatgtgcagt agtactggca
     9061 aacatataat gcgcatacat ataagcaagc acgaggatta cgcccgcagc cgtgccgcta
     9121 acgcccaaac cgccgacact ttccgccaac actccggaga accatttaat cagtccgagt
     9181 ttatttaaaa atgcggccat cataatcaat gcgccaaacc aaataatcgt atcccacgca
     9241 cttttttctt tcaaaacatc gtcccaagtc aatacaccgg aaagcaaaag caggcttaat
     9301 ccgataaatg cggtggcggt ggcgttgata ctaaaagcgt gattgccggt aataagggcg
     9361 ggaacatctg cccacaacag cagcaagata ccgaaaatga ccgccataat gatttcgtct
     9421 gccgacattt tacccatctc cctcagacgg tctttggcaa attgaacggc attgggcgtt
     9481 tctttaattt caggcggata caaaaaatat aaaatcaaag gcataacgaa aaaggcgata
     9541 acgccgggaa cagccattgc ccacgcccac gccccccaag aaagacggaa actactgcct
     9601 aaattttcgg caatcaagtt gacgattaaa gggttggggg cagttgcagt aataaacata
     9661 gccgacgaaa tgggattgga atgatagttg accaaagcca aatatttacc catcttgcct
     9721 tctgtgcctt ttgcgggatt ggagccgtaa ctgccggcaa tcgactgcat aatcggatgt
     9781 ataatgccgc cgccgcgcgc ggtattggaa ggggtaacgg gagccagcag cagttcggaa
     9841 agagcgagac tgtaaccgat gcccagcgtt tttcttccaa aaacggcgat aaacaaatat
     9901 ccgatacgca tccccagccc tgttttgagc aaaccgcgcg aaatcataac tgcgatggca
     9961 atcagccaaa tcaacggatt ggcgaacgca ctcaacgcat cgctcatcgc cgcgcccggt
    10021 ttgtcggcgg ttacgccggt tactgcgacc aacccgacgg caataatcga cagcgcgccc
    10081 aacggcatgg ccttgccgat aatggcggca atcacaccga caaacatggc cagcagcgtc
    10141 caagcctgag gcttgacccc gtcgggtacg ggcagtgcca aaaccagggc gcacaatact
    10201 gcggcaatgg cgaggggtat cggtttgaaa cccaatttca tcatattgac ctccgtaaaa
    10261 aagaccgtcc cgaaaaatcg gaaaaataat atttaactaa ttgttttata agatatattc
    10321 cgatatttca ccgtctttcc gatatgcggc tccgggcaac ttttgtttca gtatttgaat
    10381 tttcattaga ctgaatacgc cgtttgaacg gcacggcgaa aacccggggg atgccggacg
    10441 ttcagtcctt tttcgcacct tgaaggtaaa gcatttccaa ggcaattgtg gcaccggcca
    10501 aagcggtaat gtcggattgg tcgtaagagg gggcaacttc tacaacatcc ataccgacga
    10561 tgtcgagatc cgtcagccca cgtaggattt ttaatgccct gtcgctgctc aagccgccgc
    10621 atacgggcgt accggtccca ggggcgaacg acgggtccag gcagtcgatg tcgaaagtca
    10681 ggtaaacggg catattgccg acggtttctt tgattttacg gacggtctct tcaacactgt
    10741 cttcattgac ttggggggcg gtcaacacag taaaaggcaa ttttttactg tgttcggtac
    10801 gtatgccgat ttgcacggaa tgtgccggat cgatgatgcc ttccttgggg gcggtataaa
    10861 acatcgtacc gtggtcgtat tcgctgccgt tgtcgtaggt gtcggtgtgc gcgtcaaaat
    10921 gaatcagtgc gagtttgccg aaatagcggg cgtgggcgcg caacaacggt agggtaatga
    10981 aatggtcgcc gcccaaactc aaacagcgtt tgccggaaga aagtaatttg ccggcgtgcg
    11041 cttccatttt ttcgacaaaa tccctgctgt cgccaaaaga aaaaaccaag tcgccgcaat
    11101 caataatgtt caggcgttcg cgcacatcaa atgtccatgg aaacctgcgg tgctcccaag
    11161 cgaggttgac ggaggcgcgc cggatggctt caggaccgaa acgcgcgccg gaacgccctg
    11221 aaaccgccat atcataaggc acgccggtaa taacccaatc ggcatgactt tcatacggca
    11281 taaaattaag cggcaggcgc aaaaacccga aattattgga aacgagggag ttgtcggttt
    11341 gtcctgccag tgtgctgtat tgcatcgtaa tgattccttg taattggttt caatcggtcg
    11401 tgatgattgg tgtttgagta agaaaatcgg gcttcagacg acatatccga tgccttgatg
    11461 cgtctattcg tcttccaaat aggtataacc attaagcccc gcttcgagtt cttttaagaa
    11521 agacatagcc tgcgaggcag gaaggtctga atgttcgatt tgttcgcgat agcgtttcat
    11581 cagctctttc ggatcttgat aaacgtattc gagcatatcg gcaacggtgt ttccttcatc
    11641 gtaatcgatg acggtaaatt gtccgtcttc ccctacaaca acatcggcag tggcagtgtc
    11701 gccgaaaaga ttgtgcatat tgccgagtat ttcctgatat gctcccacca taaaaaagcc
    11761 taaaagcggc ggctcttctt cgggataatc aggcataggc atcgtaccgg cgatgccgtc
    11821 gccgtcgatg tagtggtcaa tcgtaccgtc tgaatcgcag gtaatgtcca acaacacggc
    11881 gcggcgcgca atcggttcat tcaaaccggt aatgggacaa acagggaaaa gttgatctat
    11941 gccccaagca tcgggcaaag attggaagag tgagaaattg acatacagct tatcggcaaa
    12001 acgttcttgc aattcgtcaa taatggttcg gtgagaccgg tgtttttcat taaacaattc
    12061 gccgacttca tgacagatat ttaaatacag ttgctccgcc cacgcacgtt gcgccaaact
    12121 caacagcccg acattatact gattatgcac atcggcaaga tcaaactgcc cttcgtgtat
    12181 ccagctgcgt aaggaacgtt tttcccgcga ggcggaaata tccgtccaag tttcccacat
    12241 actgtgcaac acacgcggcg cttcgggcga tggcgcatcc agccggcgcg gtttgtaacg
    12301 ttcaacgcct ataacattag caaccaaaac ggcgtgatgt gcggtaatgc cgcgcccgct
    12361 ctcggtgatg attgtcggat gcggcagccc gtgttcgaga caagcctgac tgatgcccca
    12421 tacgactgtg gcggcatatt cgttgaggct gtaattaacg gaacaatccg attgtgtgcg
    12481 gtttccttcg taatccacgc caagcccgcc gcctacatca aaacagcgga tatttacccc
    12541 cagtttgtgc aactcaacat aaaaccgagc cgattcgtgt acacctgtgg caacatcacg
    12601 gatgttccca agctgcgagc ctaaatggaa atgcaaaagc tgcaggcagt ccagcctgtt
    12661 tttttgtttc aaaatatcga ccagttgcaa aacttgggaa gccgacaagc cgaattttga
    12721 tttttcccca cccgaagact gccattttcc cgaaccttgg gaagccagtc tggcgcgcac
    12781 acccaaacgg ggcttgatgc cgagtttttc cgcctcttcc aataccattt gtatttcgga
    12841 cagcttctca atcaccaaat aaacctgatg ccccagtttt tcgcccatca aggcgaaacg
    12901 gatatattca cggtctttat agccgttgca gacgattaat gtttgccggg tgccggcgtg
    12961 tgccaaaacc gccatcagtt cggctttaga accagcttcc aaaccatgcg gttgtccgct
    13021 tgacataagc gattcgatga cgcggcggtg ttggttgacc ttgatagggt aaaccaaaca
    13081 ataaccgccc ttatagccgc actcttcccg tgccgtctga aaggcgcggt taatgtcgcg
    13141 gaggcggtgt tcgaggattt gcggaaaaca aaacaaaaca ggcaggcgcg cctgatgttt
    13201 ttgttgcacg gcttcagtca gtttttgcag tgaaacaatt tgattgtgtt gcgagggatt
    13261 ggggcggacg atgatttcgc cagaatcgtc aacatcataa taacctatgc cccaatgatt
    13321 aatgttgcac acttcacgga tggtaaggat aggcataata aacctgctcc gtctgtcgtg
    13381 ttgaaaagga atgattataa caaatcagcg tgaaatgtca tttttttaat aagaaaagcc
    13441 tgcctcatac ctgataagga acaggcaaaa tgccgtctga acgcttcaga cggcattttg
    13501 gttcagcttg ccatcaaagg agtcagcgat cgagctgctc tttgatgatt ttcaggtcgg
    13561 ggtaagacaa cacgatgtcg tattcgcggc cgtctttata ggcttccact tccaaaacag
    13621 gtttgcccca atggtcgtcg gcatcgacat cgtgaacctg ataaccgcgc tgctctaaca
    13681 ttttcacggc ttttgtgcgg ttttgttcaa aatggggatc accgtaaatc tgacgctcgg
    13741 cagagtcgcc ggcaaatgcg gcagcggcac tcagagaaac aacggcagcc aataacagtt
    13801 ttttcatttt cagtcctttt tttatcggtt gattgaacaa gatgtgtttt tcaataccgc
    13861 cattaaaaca cagcaaaatt aggtttgaat tagagggaag tcaagaagcg taaatgccgt
    13921 ctgaaaaaaa cagagccgtc aaacggctcc gttttcctta tgcttctttg gtttcagcca
    13981 ccttttggcg caggcgcagg ctcaattcgc gcaactgctt gtcgtccacg ctgttgggtg
    14041 cattggtcag cagacattgt gcacgttgtg ttttcgggaa ggcaatcaca tcgcggatag
    14101 attcggcgcc ggtcatcagg gtaaccagac ggtcgagacc gaatgcaagg ccgccgtgag
    14161 gaggtgcgcc gaatttcagg ttgtccaaga ggaagccgaa tttttcttgt tgctcttcag
    14221 ggctgatttt cagcgcggca aacactttct cttgtacgtc tgcgcggtgg atacggatag
    14281 agccgccgcc gatttcccag ccgttcaata ccatgtcgta ggcacgtgcc aaacaatttg
    14341 ccggatcgga aaccatcagg tcttcatgac cttctttagg cgcggtaaac ggatggtgta
    14401 cggcaacgta gcggtcggct tcttcgtcgt attcgaacat tgggaagtcg acaacccaca
    14461 aaggtttcca ttcgtctgtg aaatagccgt tgtctttgcc gtgttccaaa ccgactttga
    14521 tacgcaatgc gccgatggct tcgttcacga ctttggcttt gtccgcaccg aagaagatga
    14581 tgtcgccgtt ttgcgcgccg gtacgcgcaa tgatttcctt cagggcgttt tcagacagga
    14641 atttcacaat cggagattgc agaccgctgt cttcgccgtt ggagaggttg cccacatcgt
    14701 ttactttgat gtatgccaga cctttcgcgc cgtagatgcc gacaaattta gtgtattcgt
    14761 cgatttcttt gcggctgaat ttcgcgccgt tcggtacacg cagagcgact acgcgaccgc
    14821 ctttcatgtc ggctgcgcca cggaagactt tgaattcttc cgttttcatc aggtcggtca
    14881 actcggtgaa tttcaagttg atacgcatgt ccggtttgtc agagccgtag tagaacatgg
    14941 cttcagagta aggcatgcgt gggaaatcgc ccaagtccac gcccaatgca tcttggaaga
    15001 cttgtttcgc catgccttcg gtgatatcca tgatttcatc ctcgtttaag aacgaggttt
    15061 ccaagtcgat ttgcgtgaat tcaggctgac ggtcggcacg caagtcttcg tcgcggaagc
    15121 acttggtgat ttggtagtaa cggtcgaaac ccgccaccat caacagttgt ttgaacaatt
    15181 gcggcgattg cggcagcgcg aagaactcgc ccggatgaac gcggctcggc acgaggtagt
    15241 cgcgcgcgcc ttcgggcgtg gagcgggtca gcatcggcgt ttcaatgtcg atgaagcctt
    15301 gtgcgtccaa gtagcggcga acgcccatag caacttggta acgcaggcgc aggttgcgtt
    15361 gcatcaccgg acggcgcaag tcgataacgc ggttggtcag gcgaacgttt tcgctgatgt
    15421 tttcatcgtc gatttggaac ggcggcgtgg cggcggcgtt caagacttcg atttctttgg
    15481 caaggatttc gattttgccg gaaatcattt tatcgttggt cgtgccttcg ggacggttgc
    15541 gtacgcggcc ggtaatgctc aaaacgtatt cgttgcgagc ggaatcggca gcggcaaacg
    15601 cttcgggcgt gtcgggatcg atcacgactt ggacgatgcc ttcgcggtcg cgcaggtcga
    15661 taaaaatcac accgccgtgg tcgcgccggc ggtgcaccca gcctttaacg gtaacggttt
    15721 ggtctaagta ttgctcgctg atcaggccgc aatagttggt acgcataaaa tcacctttta
    15781 ttgatttaaa ctgaaaacag aaaatgccgt ctgaacggcg gctttattgt tgttcgggca
    15841 aatccgcctt ttcagacggc ataggtcctg ccaatgtttt gacgggcagg tcgtcaggaa
    15901 tgaccatacc cagcgaaatc acatatttca acgcttcgtc cacgctcata tcgagttcgc
    15961 gcacatcgct tttctttacc ataatatagt aaccgccggt cggattcggc gtggtcggga
    16021 catacacgga aagataatcg ccgtccttcg gcaatgcggc cttaaccgca ttcgacacct
    16081 gccctgacac gaaagcaatc gtccaaatac ccgattgggg aaacggcacg agtaccggcg
    16141 ttttaaacga gcggctgctg tcggacagca gcgattcgga tacttttttc acactcgagt
    16201 agatggattt gacaaccgga atccgcccca acaggctgtc ccacgcggca agaatctgcc
    16261 ggcccaatac gttggcggca aataatccgg ttacaaacaa tacggcaatg gcaacaataa
    16321 cgcccagccc cgggatattg aagcccaaaa catattgcgg ccgccattgc ttcggcagca
    16381 ggttgacgag ctgatcggac gcggaaacga tataggaaac cacccaaacc gttaccgcaa
    16441 tcggcagcca gaccaaaatg cccgtaatca gatatttttt taacgccttg gcagctttac
    16501 cgccttcggc cgcaggttcc gtcatcttgc ttgattccga caaagtccgt acaaaccgca
    16561 cattatacgc gtttgcccgg attcaaacga aatttttatc ccgccccgcc aaaccgccgg
    16621 cggcttcaga cggcacggca acttgatata ccgtctgaac acgcggttca gatgccgtcc
    16681 aagtcgttga acatcaaccc gataccgata ccgttctgct tgtggttgta gtcgatcagg
    16741 ctctcgccgt aaccgtggaa tccgcgtacc acgcctttga gtttgccctt aatcggaaac
    16801 gtgtaggcgg cttcaatcgc gccgtagccc gttttggggt tgtagcgcaa tacggaatac
    16861 acattctgcc tgtcgttcag gcggtactgc agcttcacgt cgccataccc catatagtcg
    16921 gcaatatcgg gattgtcgtt tttatcgccg ctctgatcga acgcacgcac ccacacgcgc
    16981 ggaatcaccg tcaatttgcc ccattccatg cctgccatgg cgtaaatcct gttccacgaa
    17041 cgcgattcgg gacggctctg tccgttggac tggtggacaa aacccgcacc gagcatacgc
    17101 agcctgccgc cgaacggcaa atccgccttc acaggctggg tcaggaaaat ttcaggtttg
    17161 taatccgtat tgcggaacgg cgcggatttc ctgccttggt tgtaaatctg ccaatcggat
    17221 ctttgggtgt agccgaacca cagatccgcg cgggttttaa acaaatcttc ggcaattttg
    17281 cttttgaacg aaacctgcaa tttggtttcc gcacgtttct gctgtccgaa tttttcctgt
    17341 acagtcgtac cgcgcgtcgg cgaactcggg gcatagttgg gcgaattgtt gtaccagagc
    17401 ggcataaggt acatcggatt gtgttcgcgt acgcccaaca gcccgcgcaa atcgtttttg
    17461 tccaagtcgt acatcaggct caaaggcgta tagatgtcgg cggtttcgcc cgcactgtcg
    17521 gcaggaagcg catccccgcc tttttcaaca acaatgaccg cctcgccctt atccaggctg
    17581 ctgcggacgg tttccgtcag attgagtaca gctttcgact cctgcccttc ctgtcctgcc
    17641 gaagacggaa gctgtgccgc aaaaatcctg tcgtaacacg ccaaacgcgt aacattgtcc
    17701 gtcaaagcgg cgcattgcag cgcggtctct ccaaaagcgg atgccatcgg caacagtcct
    17761 gtcaaaagaa tatagcgcat attccgtgta ttcatctccg cccccattgt cggcatattg
    17821 gttttcaaac ggcattttat agcggattcg gataaaaaac cgcacccttt ccgccatttc
    17881 gggatttagc ccccgtaata cagaaaaccc cgagaccgtc aggcttcagg gttttccgct
    17941 tatcgcgtat caaccgcctt ggcggttttg caaaaatcaa gccagtgctt ttacttttgc
    18001 agacagacgg cttttgtggc gtgccgcttt gtttttgtgg aacacgccct tgtcggcgat
    18061 gcggtcgatg actttgacgg actcttggta aaccgcttgt gcggcagctt tatcgcctgc
    18121 ttcgactgct ttcaatactt ttttcactgc agtgcggaat gcggtacgca ggctagcgtt
    18181 gtgggcgcgt tgtttgacgg actggcgggc acgtttacgt gcttgtgcgc tgtttgccat
    18241 attggaatct cctagaagaa aaataaattc tgtaaacgcg caattttaaa gggggtttac
    18301 attatatgca agccttttct ctctatccgc ccgtcggaca ccctgacagc gcaaaaaaac
    18361 ggcaaaatcg tgcggacgtt ttccccctct tttgaaaaca gggtaatttt ttagggaaat
    18421 cagtccaata tcactttgag tttgcacaaa tttactttac aatgcccaaa ccttgcgccg
    18481 gggtgtttat ggggcgcggg gttttctttt tttccaacct ctttcatcag gaatctgccc
    18541 gtatgaaaaa atccgtatta gccgttttgg ccgcgctgtc tctggcagcc tgcggcggca
    18601 gcgaaaaaaa cgccgtgcaa cctcaagcag acgctgcgtc tgctgccaat gccgaagccg
    18661 ctgccaccga tactctgaac atctacaact ggtcgaacta tgttgacgag agtacggtcg
    18721 aagactttaa aaaagccaac aatctgaagc tgacttacga cttgtacgaa aacaatgaaa
    18781 cgctggaagc caaaatgctg accggcaaat ccggttacga tttggttgtg ccgggcatcg
    18841 ccttcctgcc gcgccaaatc gaggcgggcg cgtatcaaaa agtcaacaaa gacctgattc
    18901 ccaactataa aaacatcgat cccgaactct tgaaaatgct ggaaaccgcc gacccgggca
    18961 accagtatgc cgtcccctat ttctccggcg tgaacacgat tgcgattacg gcgaaaggca
    19021 aagagctttt gggcggcaag ctgcccgaaa acggctggga tttgctgttc aaacccgaat
    19081 acacccacaa gctgaaatcc tgcggcatcg ccctgtggga caccccgagc gaaatgttcc
    19141 cgattttgct gaactacttg ggcaaagacc ccaaaggttc gaatcctgaa gatttgaagg
    19201 cggcggcgga agtgttgaag tctatccgtc cggatgtcaa acgtttcagc ccgtccatca
    19261 tcgacgagct ggcgcgcggc gacatctgcc tggcggcagg caacggcggc gatttgaact
    19321 tggcgaaagc acgttccgaa gaagtgaaaa acaacgtcgg catcgaagtg ctgaatccga
    19381 aaggcatggg cttctggatt gagtcttggc tgattcccgc cgatgcgaaa aacgtcgcca
    19441 atgcccacaa atacatcaac tacacgctcg accccgaaat cgcggcgaaa aacggcatcg
    19501 ccgtaacctt cgcccccgcc agcaaaccgg cgcgcgaaaa aatgcctgcc gagctggtga
    19561 acacccgttc catcttcccg aacgagcagg atatgaaaga cggtttcgtg atgccgcaaa
    19621 tgagtacgga tgcgaaaaaa ctgtctgtca gcctgtggca gaaaatcaaa gtcggcacca
    19681 actgatttga agcattaaaa atgccgtccg aacatcgttc ggacggcatt ttatattgga
    19741 ttgaaataga aat
//
LOCUS       DAXHSJ010000023        19104 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000023 DAXHSJ010000000
VERSION     DAXHSJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19104
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.023"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..270
                     /locus_tag="ACFM8U_000597"
     CDS             <1..270
                     /locus_tag="ACFM8U_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6333880.1"
                     /translation="VETTTETIFTTPTGGATLGGTIPNKSSSKPPYHQSHSISSLGLG
                     VIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
     gene            complement(244..453)
                     /locus_tag="ACFM8U_000598"
     CDS             complement(244..453)
                     /locus_tag="ACFM8U_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333881.1"
                     /translation="MPSERLSDGIFPCVLNRVHETPDAFGGNVGCRTMFGGDQRTKYA
                     GKGLMPSEGSDGIGVGESEVVAHAQ"
     gene            complement(471..1028)
                     /locus_tag="ACFM8U_000599"
     CDS             complement(471..1028)
                     /locus_tag="ACFM8U_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6333882.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDVAELEQIFQAATQVPDHGNMCP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1104..2738)
                     /locus_tag="ACFM8U_000600"
     CDS             complement(1104..2738)
                     /locus_tag="ACFM8U_000600"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6333883.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3019..4125)
                     /gene="serC"
                     /locus_tag="ACFM8U_000601"
     CDS             complement(3019..4125)
                     /gene="serC"
                     /locus_tag="ACFM8U_000601"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC6333884.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTRDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4490..4921
                     /gene="rimP"
                     /locus_tag="ACFM8U_000602"
     CDS             4490..4921
                     /gene="rimP"
                     /locus_tag="ACFM8U_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC6333885.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4949..6451
                     /gene="nusA"
                     /locus_tag="ACFM8U_000603"
     CDS             4949..6451
                     /gene="nusA"
                     /locus_tag="ACFM8U_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC6333886.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMNHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6463..9351
                     /gene="infB"
                     /locus_tag="ACFM8U_000604"
     CDS             6463..9351
                     /gene="infB"
                     /locus_tag="ACFM8U_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC6333887.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNSSTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPIAAETKPAEESKAEKAQADKMPSKKPAKPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKSAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LSSLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
     gene            complement(9455..10795)
                     /locus_tag="ACFM8U_000605"
     CDS             complement(9455..10795)
                     /locus_tag="ACFM8U_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HFC6333888.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELIILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRIGTGAATGALIGLGLDIATFGGSLGLGTAI
                     GGFLGGILPNTRTISDKLTGRQTLHTDPETLTLLAARALGLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGK"
     gene            complement(10857..12206)
                     /locus_tag="ACFM8U_000606"
     CDS             complement(10857..12206)
                     /locus_tag="ACFM8U_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC6333889.1"
                     /translation="MLNPSQKLVELVRILEEGGYIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRVGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLIL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWVVCKIFLKTSQ
                     SKLDLEKPYYQAVIRRWQNKITDADTHQETVSAVSPKIILNDAPKWAVMLETGWHEGT
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLAEKLEHWRNALTECGAAWLEPDRVAQEVRLKTNDRT"
     gene            12350..12976
                     /locus_tag="ACFM8U_000607"
     CDS             12350..12976
                     /locus_tag="ACFM8U_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC6333890.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSI
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYVVMGWMVLAVMKSLTASLPLAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13040..14458)
                     /locus_tag="ACFM8U_000608"
     CDS             complement(13040..14458)
                     /locus_tag="ACFM8U_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6333891.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            14785..15513
                     /locus_tag="ACFM8U_000609"
     CDS             14785..15513
                     /locus_tag="ACFM8U_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC6333892.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     SNPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15572..16060)
                     /locus_tag="ACFM8U_000610"
     CDS             complement(15572..16060)
                     /locus_tag="ACFM8U_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC6333893.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRGRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(16818..17282)
                     /locus_tag="ACFM8U_000611"
     CDS             complement(16818..17282)
                     /locus_tag="ACFM8U_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC6333894.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            17504..18562
                     /gene="alr"
                     /locus_tag="ACFM8U_000612"
     CDS             17504..18562
                     /gene="alr"
                     /locus_tag="ACFM8U_000612"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC6333895.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLIRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGGDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT         5054 a         5250 c         4806 g         3994 t
ORIGIN      
        1 gtggaaacaa caaccgagac tattttcact acaccaacgg gaggcgctac actgggaggc
       61 actatcccaa acaaatcgag tagcaaacct ccctatcacc aaagccacag tatcagcagc
      121 ttggggcttg gtgtcatcgc cggtgtcggt ttcgacatca cgcccaagct gaccttggac
      181 accggatacc gttaccacaa ctggggacgc ttggaaaaca cccgcttcaa aacccacgaa
      241 gtctcattgg gcatgcgcta ccacttctga ttccccgaca ccgatgccgt ctgaaccttc
      301 agacggcatg agacctttgc ctgcgtactt ggtacgctgg tcgcctccga acatggtgcg
      361 acacccgaca tttccgccga acgcatcggg cgtttcatga acccggttta aaacgcatgg
      421 aaaaatgccg tctgaaagcc tttcagacgg cattttgctt gagattccgt ttaccaatga
      481 ctgacaaacg cttccaaatc ggtattcttg ggcttatgca cttcctctgt cggcgtgccg
      541 accatcatca gcccgatgat tttatcctta tccgcacatc cgaaagcctc acgcaacaag
      601 gggctgttga cccacatccc cgttatccag acattgtcga aaccttgcgc cgttgccgcc
      661 agttgcagcg catacgccgc acaacccgcc gtcagcatct gctcccattc cggtttcggc
      721 ttaggcacat cgcggttcgg cgcaaaggtt accccgataa ccatcggcgc catattgccc
      781 actttttccg cctttttcat cgcatcgtcg ccgaaattca attcggcaac cgtttgcttt
      841 aacacatcgc gaaaacgttg caatcctacc tcgccttgaa tcacggtaaa acggaagggg
      901 cacatattgc cgtgatcggg aacttgggtt gccgcctgaa atatttgttc caactccgcc
      961 acatcggggg cggggggctt cagctttttg gaagatcggc ggttcgtcaa taattttaaa
     1021 gcatccatat cgttatactc cggtcatcgg tcgggtgttc ggacacatat gccgtccgaa
     1081 ggcttcagac ggcatatccg gcatcagcgc ggacggcggc aggctgccaa tatatccatt
     1141 tccttccgat aggtttggct attggaaata tccatcagcc ccaataccgt gctgaaatag
     1201 tggtcgtgcg aatattcgtt ttccgccgct ttttgtttga ggcattggaa atctatgccc
     1261 ccgtgttggc ggaaggcttt ggaaaaccac ataaccatcg ggatatgcgt ctgcccggaa
     1321 ggcgcgatgg cgtaaggcgc ggcgtgcagg tacatcccgt tttcgcccaa actttcgccg
     1381 tggtcggaaa cataatgcac cacgctttcc aaatcgtcgc ggttttcaag tttgcggata
     1441 accttgtcga taaactggtc cacatacaaa accgtattgt cgtaagtgtt gaccagcgtg
     1501 gcgcgggtgc atttgttgat ttcgttggtg tcgcaggtcg gcgtgaattt gcgttcggct
     1561 tcggtatagc gttcgtaata cgtcggcccg tggctgccga tggtatgcag gattaaaacc
     1621 gcgtctttat cgtttttgtt gaggacttcg tcgaacttag tcagcaggat attgtcgagg
     1681 cactcgccgt tgcggcagta ttcgggcagg ttgagcgagg taacgtcggt attcggcact
     1741 ttgccgcaca cgcccttgca gccggaatcg ttttccaacc aagtaacttc cacgccggcg
     1801 cgctgcacga tgtccagcag gttgtcttgg tgttcggctt tgatttcgtc ataatccgtg
     1861 cggtcgaagg ttgagaacat acacggcagg gagtgcgcgg tcgatgtgcc gcagcttctg
     1921 acctgcggga aattgacaat ttcatcgccg cgcgcggcaa gcagcggcgt agtttggcgg
     1981 ctgtaaccgt tcaaacccca gttggcggca cgcgtggtct cgcccacgac cagcaccacg
     2041 aaacggcgca ggctgccggc cggccggttt tgcacgaccg ccatatccaa ttgcgtataa
     2101 ggaatattgg aacgcttcca atctttgtat ttcgacacgc ccgcgccgat gaaattagac
     2161 ggcacaatca gatgggttac tgatttattg ttgcggaaaa acgaggcgta atcctgatat
     2221 tgcaacattg cgatgcccaa cgcgcacaaa aaggaaacgg cggcaagcac aaggcgtgtc
     2281 aaaagctcct tataccaaac gcggtattta accttgacgg cgatatacgc cagcgcgggc
     2341 aatacgccca aacatacaat ccacagcaca tagcccggcg taatcaggcg cgcgctttcg
     2401 gcagccgtag tttgcaagac attattcaac atcgacttgt tgaaatagat attgaaaaat
     2461 atttcttggt aagacaccgc cgcactgata accaatatca acggaatcaa taccttatgc
     2521 acgaaaggca gggcaatgac gtgaaaaacg aaattactta aaaaaaacag caccaccggc
     2581 atcgtataga ggaagatatc cgccccggtg ccgttaaaag gatgaagctc gacaactttg
     2641 gcaaaaaagg cgtaattcaa taccagcgag gaatacaggg aaaggaaggc aatcagcgcg
     2701 gaagagccga gcttcggcct caggttcggt tttatcattt ggaatgtgtc ggataagggt
     2761 tggaaaaggc atccggcatt tggaatccgg attattgaaa aagattctta attataaggc
     2821 aacggagcaa agcagggcaa gaaaacggaa ggcgacgcag gctgtcggat acacatctcg
     2881 gcacaaacag catcacgccc ggattgaaaa ggacggatag ccgccggcag cacagtttta
     2941 ccccgcatcc caaaacgccg ccggagaacg ccgaaacagc cgatgccgtc tgaagccgct
     3001 tcagacaaca tcgggacatc aaccgtaacg ccgttggaaa tcgcgcataa aatctgccaa
     3061 agcccgcacg ccttcgagcg gcatcgcatt ataaatgctg gcacgcatcc cgccgacaga
     3121 cttatagccc ttcaacaggc acaagccctg caattcggct tccagcacaa aacggcggtc
     3181 aagctcctca tccctcgttt ggaacacgac attcatttta gaacgcgcat tcggacggat
     3241 acggttgata tagaaaccgc cgctgccgtc tatcgtctca tacaaggttt gcgccttcag
     3301 cctattgacc gcttcaattt ttttcacacc gccctgcgcc tgcagccagc ggaacaccag
     3361 ccccgacata taaatcgcgt aagttgacgg cgtgttgtac ataccgtcgc ggttcaaatg
     3421 cgaacggtag ttgaacacat cgggaatatc gttcggacaa cgctcgagca aatcctcacg
     3481 cacaatcacc accgtaaccc ctgccggccc gatgtttttt tgcgcgcctg cgtaaatcaa
     3541 gccgtaatcg gcaacatcaa actcgcgcga caaaatctcg ctggacatat cgcacaccag
     3601 cggcggcatg ccttctgaaa ggcgcggcac ttcacggtat tgcagcccgt tgaccgtttc
     3661 attgacggca aaatggacaa acgccgaatc gggtgcaaca tcccacgttt ccacaggcgg
     3721 caggtcgaga tagtcgaact gctcgccgcc atgcgccgcc aaacggattt ccgtatcggt
     3781 caaacggctc atctgttcat aagcgatacg gctccagttg cccgttacca ccgcgtcggc
     3841 agtgcggaaa ccgtgtgcca gattcatggc tgccatatta aattgggttg ttgctccgcc
     3901 ctgcagaaac aatatcttat agttgtcagg cactttcaaa agctgcctca aatcctgttc
     3961 cgcatgatgc aggatgctca aaaacatttc cgaacggtgg ctcatcgcca tcacgggaaa
     4021 acccgtaccg ttgtagtcca acatttcctg ccgcgccgtt tccaacacgg cttcgggcaa
     4081 tacggcaggg ccggcggaaa aattgtaaat cggataaaga gacatgatgc agccttgatt
     4141 ctgaacaata acccgcccga ttttaggctg gcagcaggct ttgtgcaagg atgggaaata
     4201 cctgtcctcc gcccgactcc atgccgcccg aacacggaaa acaacatcaa tatattgatt
     4261 tacaaatata aaaatcatgc tagcgacaaa tagatacatt tgttttgtca acaatattca
     4321 cgatttccca ttacaaacct cccttacacc cgcttttttc cgtcccaaaa acacaaaata
     4381 aatcaacact ttcatttctc cgcaaaagcg gttataatca cgccgatttt tcaactttga
     4441 cgaaaaatgg ccacggggcc atttttttat tatgtacata gggagcagca tggatatcca
     4501 aaccatcctc gaaaaaaccc tgcccggcct gggctacgaa ctggtcgatt tcgaactgac
     4561 cgcgcaagga acattgcgcg tgttcatcga caaagaaagc ggcattaccg tcgaagactg
     4621 cgcaaccgtc agcaaccact tgagccgcgt cttcatggtt gaagacatcg actacaaaaa
     4681 cctggaaatt tccagccccg gactcgaccg ccccttgaaa aaagccgccg acttcgtgcg
     4741 ctttgccggt cagaatgcca aaatcaaaac ccgcctgccg atagacggtc agaaaaactt
     4801 tatcggtaaa atcgaaggct gcgaaaacga taccgttacc gtatccttcg acggcaaaac
     4861 cgtacaaatc gaattgggca acatcgacaa agcccgtctg cgccccgaat tcaaattcta
     4921 aaacacaaca atattggaga tgttcaaaat gagtcgtgaa atgttacagc tggcagaagc
     4981 actggcaagc gaaaaaaacg ttgatgcgga agtcgtcttc caagcactgg aattcgccct
     5041 gtctaccgcc gccaagaaaa aggcagaccg cgaacacatg gacgtgcgcg tccaaatcaa
     5101 ccgcgacacc ggcgaatacc aaaccttccg ccgctggctg attgtcgccg atgaagacta
     5161 tacctatccc gatgtcgaaa aaaccatcga ggaaatccaa gaggaaattc ccggcactac
     5221 catccaaatc ggcgaatact acgaagagca gctgcccaac gaaggcttcg gccgccaagc
     5281 cgcgcaaacc gccaaacaaa tcatcctgca acgcatccgc gatgccgagc gcgagcagaa
     5341 tctgaacgag tttctcgccg tcaaagaaga catcgtgtcc ggcacggtca aacgcgtcga
     5401 acgccacggc atcatcgtcg aagtcgttgc cggcaaactg gacgcgctga ttccgcgcga
     5461 ccaaatgatt ccgcgcgaaa acttccgcag cggcgaccgc atccgcgccc tcttcctgcg
     5521 cgtcgaagaa atcggcaaca ccggccgcaa acaagtcatc ctgagccgta cttccggcga
     5581 tttcctcgtc aaactgtacg ccaatgaagt acctgaaatt gcagacggca tgcttgaaat
     5641 ccgcgctgtc gcccgcgacc cgggacaacg tgccaaagtc gccgtcaaag ccaacgacca
     5701 gcgcatcgat ccgcaaggca cctgtatcgg cgttcgcggt tcgcgtgtca atgccgtcag
     5761 caacgaattg tccggcgagc gcatcgacgt tgtcctttgg tcgcccgagc ctgcccaatt
     5821 cgtcatgagc gcgctctcac ccgccgaagt cagccgcatc gtgatcgacg aagacaaaca
     5881 cgccgtcgat gtcatcgttg ccgaagacca gctcgcgctc gccatcgggc gcggcggtca
     5941 aaatgtgcgc ctcgcctccg atttgaccgg ctggcagctc aacatcatga cttccgccga
     6001 ggcagacgaa cgcaatgcgg cagaagatgc cgccatccgc cgcctgttta tgaatcactt
     6061 gaacgtagac gaagaaactg ccgacgtact ggttcaggaa ggttttgcaa ccttggaaga
     6121 agtcgcctat gttcctgccg ccgaactgct tgccattgaa ggatttgacg aagaaatcgt
     6181 cgatatgctc cgcaaccgcg cccgcgatgc catcctgacc atggcgattg ccgccgaaga
     6241 aaaactgggc gaagtatccg acgatatgcg caacctcgaa ggcatagatg ccgatatgct
     6301 ccgcagcctt gccgaagcag gcattaccac ccgcgacgac ttggcagagc ttgctgtgga
     6361 cgaactgatt gaaatcaccg gtgtaaacga agaaaccgca aaagccgtca tcctgaccgc
     6421 acgcgaacac tggtttaccg aagacaaata aagggggtac agatgagtaa cacaaccgta
     6481 gaacaatttg ccgccgagct gaaacgcccc gtcgaagacc tgttgaaaca gttgaaagaa
     6541 gccggcgtca gcaaaaacag cggcagcgat tccctgacgc tggacgacaa acagcttctg
     6601 aacgcctacc tgaccaagaa aaacggcagc aacagcagca ccatcagcat ccgccgcacc
     6661 aaaaccgaag tcagcaccgt tgacggcgta aaagtcgaaa cacgcaaacg cggacgcact
     6721 gtcaacattc cttctgccga agaattggca gcacaggtaa aagccgccca aacccaagcc
     6781 gcacctgtcc ggccggagca gacggcagaa gacgcggcaa aagcccgagc cgaagctgcc
     6841 gcacgtgcag aagcccgtgc caaggcagaa gcggaagcgg caaaactgaa agcggcaaaa
     6901 gcaggcaaca aagccaaacc tgccgcgcag aaacccaccg aagcaaaagc cgaaaccgca
     6961 cccattgcgg cggaaaccaa acccgccgaa gaaagcaaag cggaaaaagc ccaagccgac
     7021 aaaatgccgt ctaaaaaacc cgccaagccc aaagaaaaag ccgccaagcc gaaacacgag
     7081 cgaaacggca aaggcaaaga tgccaaaaaa ccggcgaaac ctgccgcacc tgccgtgccg
     7141 caacccgtgg tcagcgcgga agaacaggca caacgcgatg aagaagcacg ccgtgccgcc
     7201 gcacttcgcg cccaccagga agccctgttg aaagagaaac aggaacgcca ggcacgccgc
     7261 gaagccatga aacaacaggc agaacaacag gcaaaagccg cacaggaagc caaaaccggc
     7321 agacagcgtc ccgccaaatc tgccgagaaa ccgcaggcag ccgcgccggc cgtcgaaaac
     7381 aagcctgtca atccggcaaa agcgaaaaaa gaagaccgcc gcaaccgcga tgacgaaggt
     7441 caaggccgca acgccaaagg caaaggcgga aaaggcggac gcgaccgcaa caatgcacgc
     7501 aatggcgacg acgagcgcgt acgcggcggc aaaaaaggca aaaaactcaa actcgagccg
     7561 aaccaacacg ccttccaagc accgaccgaa cccgtcgttc atgaagtttt ggttcccgaa
     7621 accattaccg ttgccgattt ggcgcacaaa atggcggtca aaggcgtgga agtggtcaaa
     7681 gccctgatga agatgggcat gatggttacc atcaaccaat ccatcgacca agacaccgcc
     7741 ctgattgtcg tcgaagaact cggacacatc ggcaaacccg ccgcagcgga cgaccccgaa
     7801 gccttcttgg acgaaggtgc ggaagcagtg gaagccgaag cattgccgcg tccgcccgtt
     7861 gttaccgtga tgggtcacgt cgaccacggc aaaacctcgc tgctggacta catccgccgt
     7921 accaaagtgg tacagggcga agcgggcggc attacgcagc acatcggcgc gtaccacgtt
     7981 gaaacccctc gcggcgtgat taccttcttg gacactccgg gccacgaagc ctttaccgct
     8041 atgcgcgcac gcggtgcgaa agcaaccgac atcgtgattc ttgtggtcgc cgccgacgac
     8101 ggtgtgatgc cgcaaaccat cgaagcgatt gcccacgcca aagcggcagg cgttccgatg
     8161 gtggttgccg tcaacaaaat cgataaagaa gccgccaacc cagagcgtat ccgccaagag
     8221 ctgacccagc acgaagtcat ccccgacgat tggggcggca cggttcaatt catcgacgtt
     8281 tctgcgaaaa aaggaacgaa catcgacgcg ctgctcgaag ccgtattgct cgaagccgaa
     8341 gtattggaac tgaccgcacc tgtcgatgcg cccgccaaag gcatcatcgt tgaggcgcgc
     8401 ttagacaaag ggcgcggcgc ggttgccaca ttgctggttc aaagcggtac gctgaaaaaa
     8461 ggcgatatgc tgctggccgg tacggcattc ggcaaaatcc gcgcgatggt cgatgaaaac
     8521 ggcaaatcca ttaccgaagc cggcccgtcc atcccggtcg aaatcctcgg cttgtccgac
     8581 gttccgaacg cgggcgaaga cgcgatggta ttggcggacg agaaaaaagc gcgcgaaatc
     8641 gccctcttcc gccaaggcaa ataccgcgac gtgcgccttg ccaaacagca ggcggcgaag
     8701 ctggaaaata tgttcaacaa tatgggcgaa acccaagccc aatctttgtc ggtcatcatc
     8761 aaggcagacg ttcagggctc ttacgaggct ttggcgggca gcctgaaaaa actgtccaca
     8821 gacgaagtga aagtgaacgt gttgcacagc ggcgtgggcg gcattaccga atcggatgtc
     8881 aaccttgcca tcgcttcggg cgcgttcatt atcggcttta acgtgcgtgc agatgcctct
     8941 tcgcgcaaac ttgccgaaaa tgaaaacgtg gaaatccgct actacaacat catctatgat
     9001 gccatcgacg acgtgaaggc ggcgatgagc ggcatgcttt ctccggaaga gaaagaacag
     9061 gttaccggta cggtcgaaat ccgtcaggtc atctccgttt ccaaagtcgg caacattgca
     9121 ggctgtatgg ttaccgacgg cgtggtcaaa cgcgattccc atgtccgcct catccgcaac
     9181 aacgtagtca tccacacggg cgagctgtcg tctttgaaac gctacaaaga cgacgtaaaa
     9241 gaagtccgca tgggcttcga gtgcggtctg atgctcaaag gctacaacga aatcatggaa
     9301 ggcgaccaac tggaatgctt cgacatcgtc gaagttgccc gcagcctgta attcctttgc
     9361 aaataaaatg ccgtctgaag cgttcagacg gcaacgaaac gggttctgta tgttgcagaa
     9421 cccgtttttt gtcgcaaatc ggcttcagac ggcattattt tcccgacagg cggatttgaa
     9481 gtttctccac atatccggcg cgttcggcgc ggctggtttc cgggcggtgc gtattgagcg
     9541 acgaccattt ccaatgactg cgggctttgt tgagttctgg cgggagtctg gcggcgtccc
     9601 aaggggcttt gcggctgtgc agctcgatat ccgactgtgc cgcgtgtccg cgcgtttgca
     9661 ggacgtggag caaaccgagg gcgcgggcgg cgagcagggt cagggtttca gggtcggtgt
     9721 gcagggtttg acggccggtg agtttgtcag aaatggtgcg ggtattgggc aggatgccgc
     9781 ccaaaaagcc gccgattgcc gtacccaatc cgagcgagcc gccgaatgta gcgatgtcca
     9841 gccccaagcc gatgagcgcg ccggttgccg cgcccgtgcc gatgcggatg ccgtattgtt
     9901 tgagcaattc gctgtcgaac gggtcttggc ggaaggcttg cggcatccag tcgccgccgt
     9961 cgatttcgct gtggtagaaa cggtagaggg caaacagccg ctgctgcatc tgccgttcga
    10021 gttggcgtat ttccgcctgc atggtttgca gcacggtggc ggtatcctcg ttttcgtcca
    10081 cttcctgcct gaaggcggcg gcatcgagca ggaaatcggc gatttcgcgg cgcgcttcgc
    10141 cgtccagccg ctgccattcg cgccggcgca tggctgtcag gcggtcaagt gtgctgcgtt
    10201 cgggcaacat ggtggcgagg ttttcccaca ggcgtagttc gccttcaaaa tcaaaggcga
    10261 cggtgtcgaa ccctgcgaaa acgtgcaggt ttctcctcgc cagcatggtt gtccacgatt
    10321 cgggaggctg tccgccggta aagttgaaca cgggcataac cggtttggcg caccatgaaa
    10381 ggataatcaa ttcatcccga tatttgtcga gaaccggctc gcgcgcgtcg atgacgtaca
    10441 ttgccatatc gctttgcaag acttgccgta agactttggc ttcctgattg aaatcatggt
    10501 gcgcgccgtg gctgccgaga aactgttgca gccgttcgat gccgtctgaa cgattgtccg
    10561 tatggttttc cagccattcc agcacgccgc ccgcgtcttc gagtccgggc gtgtcataca
    10621 ggaaaaccag catgtccgcg ccgtcgctga tggcggcttc ttcgacatgg cgcgtggtcg
    10681 atggggcgtt tctgacttcg ccgaaaccgc tgtcgcgcaa aagggtacga aggagcgagg
    10741 ttttgccggt attggtatgc ccgacgacgg cgagggaaag gggttgtttg ttcatgatgt
    10801 ttttgaagaa tggattttca gacgaccttt taaggatggc ggctttatgg ggcgtttcaa
    10861 gtgcggtcgt tggttttcag acggacttcc tgcgccaccc tgtcaggctc aagccacgcc
    10921 gcgccgcatt cggtcagcgc gttacgccaa tgttccagct tttccgcaag gtcgtctgaa
    10981 agcccctgtt ccgccaaaag ctgcaccacc gcgccgccct gcgccgcttc cgagagccgg
    11041 acaatctgcc gcaacacgcc ccggtccggc acagtttggg cgcgtacgcc gataagcagt
    11101 tgcgccggtt tctgcttcag ctctgtctcc agcgcggcaa cctgttcccg attggcggca
    11161 acgcccttat ccagccattc ctgcgccagc ctgccttcaa accacgtccc ttcgtgccac
    11221 ccggtttcca gcatcaccgc ccacttgggc gcgtcgttta agatgatttt cggagaaacg
    11281 gcagacacgg tttcctgatg cgtatccgca tcggtgattt tgttctgcca acggcggatg
    11341 accgcctgat aataaggttt ctctaaatcc agcttacttt ggcttgtttt caaaaagatt
    11401 ttacacacta cccaagccag caggcgcggc aggatgccgt agcaggcgat actgccgacc
    11461 agcagccccg accaagcccg cgcatcggca atattgccgt tcagacggcc ttcgatgacc
    11521 gcccgcgcat caggcacggg aaaacccagt ttcgcaggca gccatgccaa catttccacc
    11581 gcgcgtaccg aagcggcatt gctcaacagc gtgctttccc agttgaacgt atattgccgc
    11641 accaaaagca gcagcaatac cgacaccagc attccgagca gcgtgcagag ccacaggctg
    11701 tgcgacgttg cgcctatttt ccaacgtacc gaaggttgcc gccactcgtc cgcatacagc
    11761 cgcaacaccg cctgattgac agggtctttg ccccgaaacc acgtcgccgg actgctgaaa
    11821 aaacgcccca ctttcacgcg caggaacaac gttgccaacc atactgctaa catcagcgta
    11881 ttcatgccca acacgcccgc caaaatcagg aagaaattca gcccctgatt gtccatcaga
    11941 agataagtgc ctgaaaatcc ggcggtaaac atcatcgatg ctgccaccac ccacaaccag
    12001 aacgacccca cacgcacacg ttccaacgtg tcccgcagca tacggttcct gtcgatcatc
    12061 tccgcccgac ggatgatttt ttcctccgta ctgccgtcca cgcggcgcaa agcctccgtc
    12121 gcctgcacgg gatcgccgct gaaaatatag ccgccctctt ccaaaatgcg aaccaattca
    12181 accagttttt gggaaggatt caacataaaa tgccatctga aaataaaaaa acagatttta
    12241 acacacgcat tttcaagaat attcacagtg taggcaaaga gtaaatccca cacagaggca
    12301 aaagtatcgg cgtaaactga ctgcctctac tttcccgaaa gattgtgcga tgtatacagg
    12361 cgaacgcttc aatacttaca gccatttgag cggtttgatt ctggcggcgg caggtttggc
    12421 gctgatgctg ctgaaaacca taggacacgg ggacggctac cgtatcttca gcgtatcgat
    12481 ttacggcatc agccttcttc tgctctattt gagttcctcg ctgtaccacg gaattgcagc
    12541 cggaaaactg aaaagcattt tgaaaaaaac cgaccactgc atgatttatg tgctgattgc
    12601 cggaagctac acaccgtttg cactggtttc tttgagaaac gggccgggct ggacggtatt
    12661 ttcactgtcc tggctgctgg cggctgcagg aatcgcacaa gaactcacca tcggacggaa
    12721 aagcgaaaaa cgactgctgt ctattgcgat ttatgtcgtc atgggttgga tggtcttggc
    12781 ggtaatgaaa tccctgacag cctcactccc gctggcagga ctggcttggc tggcggcagg
    12841 cggtatgctg tacagcgtcg gcatttactg gtttgtaaac gatgaaaaaa tccgacacgg
    12901 gcacggaatc tggcatctgt tcgtattggg cggcagcatc acccaatttg tcagtgtgta
    12961 cggttatgta atctgaatgc cgtctgaaaa gcaaaacctc ccgatcctga agattgggag
    13021 gttttctgtt tgccggacat cagcccttgt cgtggaactc gtgaaattca tactgatagg
    13081 acaaatcccg acccgctttt ttctgtgcca aataatcatc ataaatggca cggatttcct
    13141 tacgcaacaa aaacagggct atcaagttcg ggataaccat aaagccgttg aacatatcgg
    13201 acaggctcca aaccaaatcg actttgccga gcgtacccaa aacgatggca agcagaacca
    13261 atgcgcgata gatgcccaag tgtcttcccc tgaaaagaaa acggatgttg gactcaccga
    13321 aataatacca gccgataatg gtcgtcaagg cgaaaaaggt cagacacacg gcaagcaatt
    13381 gcgaaccgaa gcccggaaat gccttgttaa aggcaaactg ggtaaccgcc gcgccctgtt
    13441 cgcccgaaag gttggcatca gtcagcagga taatcaatgc cgtagccgta cataccaaaa
    13501 tcgtatcgat aaacacaccg acaaatgccg ccataccttg ctgcacagga tgcttcacat
    13561 cagcggttgc atgtgcatgc ggggtagaac ccatacctgc ttcgttggaa aacagaccgc
    13621 gcgccacgcc gaaacgtatc gcttcacgca taccgatacc cgcagcaccg cccaaaacgg
    13681 cttcaggatt gaaggcggcg gtaaagatgt ggttgaacat cggcacaata tagtcggaaa
    13741 attcaaacag gataacgacg gcgcacaaaa tataaacaac cgccataaac ggcacgacaa
    13801 actgggcaat attggcaata cggttcacgc cgccaatcac aaccatgccc gcaaggacgg
    13861 caagcacaat accgactgcc aaagaaggca catcaaatgc aatggtaacg gcagaagcaa
    13921 tggagtttgc ctgtgtcgca ttaccgataa agcccaatgc gataatcaac gcaatggaga
    13981 aaaagcccga caggaaacgc gccgcgcccc tgccgatttt cggagtcaga ccgtgcgtaa
    14041 tataaaacgc cggcccaccg atgtatttgc cgtggctgac gacgcggtat ttctgcgcca
    14101 gcagtgcctc cgcaaaaatc gtggacatcc ccaaaacggc agaaacccac atccaaaaaa
    14161 tcgcgcccgg cccgcctgcg gtgatggcgg tcgccacgcc ggcgacgttg cccgtaccga
    14221 tttgcgcaga tatggcaacc gccaacgcct gaaactgcga taaagacttg tcgtctttat
    14281 cgcctttggc aaacaagccg ccgaatacgg atttgaatcc cgcgcccagc ttggtaatct
    14341 gcggcgcacc gagatacagc gtaaaaaaca ggccgatacc caaaagcgcg taaatcagca
    14401 ggtagtccca aaggaaccga ttgactgtac ccaccagaac agacaatata ttttccataa
    14461 aataaacctt atcttacaat taaaatgact gccttccaaa agacattcca ataaggaaac
    14521 acggcgagca gaccgtattt gccgcaacag atgccttaaa ttgtcaacaa tcggggagaa
    14581 gctgcgccat cataccgtaa aatatcgtta attaaaacat ttctttattt ttaagcggaa
    14641 agcggaggaa atcgctttca gacggcatcg acaacggcac ggcataaaac aggatatttt
    14701 gggtacttgc aacttatgtt aaaatgccga ccgtaaaaaa tctgacaaaa acaaattaat
    14761 tatttgaaat aagaaaggaa atttatggca ggccatagca agtgggcgaa tatccagcat
    14821 aaaaaagccc gtcaggatgc caaacgcggc aaaatcttca cccgtttaat caaagaaatc
    14881 accgttgcgg cgcgtatggg cggcggcgac cccggttcaa atccgcgcct gcgcctggct
    14941 ttggaaaaag cagccgaaaa caatatgccc aaagacaatg tgcaacgcgc catcgacaaa
    15001 ggtacgggca acttggaagg cgtggaatac atcgagttgc gctacgaagg ctacggcatc
    15061 ggcggcgcgg ctttgatggt ggactgcctg accgacaaca aaacccgcac cgttgcggac
    15121 gtacgccacg cgtttaccaa aaacggcggc aacttgggta ccgacggctg cgtggcgttc
    15181 aacttcgttc atcaaggcta tttggtgttt gaaccgggcg ttgacgaaga cgcgctgatg
    15241 gaagcggctt tggaagccgg tgcggaagac gtggttacca acgacgacgg ttccatcgaa
    15301 gtcattaccg cgccaaacga ttgggcgggc gtaaaatccg ctttggaggc ggcaggttac
    15361 aaatccgttg acggcgacgt tacgatgcgc gcccaaaacg aaaccgaact ctccggcgac
    15421 gatgccgtca aaatgcaaaa actgattgac gcgctggaag atttggacga cgtgcaagac
    15481 gtttacactt ccgccgtatt gaatctggac tgatacgcag cacagcagac atacaataaa
    15541 atgccgtctg aacctttcag acggcattga tttatttagc ccttgcccac gcccaccaaa
    15601 ccgtcaggac aaccgccaga aaatacgccg cgctccaaac aggcaaagca acccctaaca
    15661 gataatccgg ttcggcacaa tttccgaacc cgcgcacgac aggctcgaac caatcaaaca
    15721 aaggccagcc cttcaatcga aacgtccacg gcgcgccgca cgaaggagcc gtacccggcg
    15781 gcagcgactg cagccacaac tgatatgccg caacagaaat acccgtaacg gccggaatac
    15841 tgataaagac agcaccgaac aaaccgcctg cccttcctct tggtctgcac atcaggacaa
    15901 ttgccgtaca caatgcggtt gccaaaacgc ataaccgctg actgatacac aaaacgcaag
    15961 gctccatacc caaaacatac tgtgccgcca aagaaccggc aaatgcacag accgaaacgg
    16021 caaacagcag ccaaacggct tttctaaata acggggtcat tttctcaaca caccaatcaa
    16081 aataccgata tgccgatttt gctggatata tcccgagatg gcaagggacg aatcgacacc
    16141 ggcgcaggtg cggacgattt cggttttccc gatatacaca agacctgaca agggacaaaa
    16201 cggcgatttg cccgcacaat gcccgcaaaa aaatacatcc ggaggatttg aatttcggca
    16261 aatttagcga atcaaaagtt tatttcaatg aaatcatatg atttttttga ataagcggat
    16321 tgacaggttt ttgaaggaat ttgttaccgg atagccatcg ggcaagtttt ttgcaaaatt
    16381 tgaatcggtc gggtaaattt tcaaaaaata attgacagcg gataagaaac ggcggataat
    16441 ttccgccgtc gagttgcttg atgcagcttg atttttctcc tctatttctc ctttgtagac
    16501 ttggcacaca ttcaactgga tgtgtgcatt tttttatctc cgcttttttt gaaatttaat
    16561 tacttttatt gtttgaaatt ccattcttta agaaatttaa caagaaaaac gcttgctttt
    16621 tgaccggaaa agctgcataa ttcattccgt tagctggttc gagtagtcag ttaatagttt
    16681 ctcctctatt tctcctttgt agacttggca cacattcaac cggatgtgtg cattttttta
    16741 tctgaagcaa caagcctctg tgcgtgatgt tgttatgttt catttaggtg tcaaaccgca
    16801 tagccagcct gaaatattca atccaaatcc aaaaccggat tttctttgac ctcctccatc
    16861 acaacataac tcctactctc cgaagcggca ggcagttgca ataggatatt gcctagcata
    16921 tcccgatagg cagacatatc gggcaaacgt actttaatca gatagtcgta ttcgcccgac
    16981 accaagtggc attccataat ttgcggaatt ttcagcactt cttttttgaa atcttcgaaa
    17041 atattgcccg atttggattg cagcttcagc tcgacaaaaa ccaatagtgg tttgcccaac
    17101 agatggggat tgagatgggc gtgatagccg gaaatataat gttcccgctc caaacggcgc
    17161 accctctctg taacgggcgt ggtggacaag cctaccttct cggcaagctc cgtcatcggg
    17221 atgcgggcat tctgttggag gatcttaaga atgcggaaat cgattttatc tagttctttc
    17281 atttggattg ccttgtattt attattgatt ttaacaaata gagtataaat ttgagaaagc
    17341 gattatatca ggaaaagcaa accgccttcc tacctgaaaa ctgctgcttc ggcttgaaga
    17401 tacaagggtc tttaatattt taaaagcctt gccgttggat tataattccc taattgattt
    17461 cttaattttg ctaataaaca cttgtttggt aaggaatgaa tttatgcggc ctttgaacgt
    17521 gcagattagg ttgggcaacc ttaggcacaa ttatcggatt ttgaaggaaa tgcacggagg
    17581 caaactgttg gcggtagtga aggccgacgc atacggacac ggtgcggtca gatgtgcttt
    17641 cgcgctggca gacttggcag acggctttgc cgtggcgaca atcgatgaag gaatcaggct
    17701 acgggagagc ggcattaccc atccgattgt ccttttggaa ggcgtatttg aagcatcaga
    17761 gtacgaagcg gtcgaacaat actcgctttg gccggcagtc ggaaaccaat ggcagcttga
    17821 ggctttgctg atccgccatt ggaaaaaacc cgtcaaagtc tggttgaaaa tggattcggg
    17881 gatgcaccgt accggttttt tccctcatga ttacgcttcg gcatatgcgg cattgaagca
    17941 gtcggaatat gtggacagta ttgtcaaatt ctcgcatttc tcctgtgcgg acgaacccga
    18001 aagcggtatg acggaaatac agatggaagc attcgatttg ggtacggaag ggctggaagg
    18061 cgaagaaagc cttgccaact ccgccgctat tttgaatgtt cccgaagcac gcagggactg
    18121 ggggcgcgcc ggtctggcgt tatacggcat ttccccgttc ggaggaggcg atgacaggct
    18181 gaagcccgtg atgaggcttt caacccgtat tttcggcgaa cgcgttttac agccgcactc
    18241 ccctatcggt tatggcgcaa cattttatac cagcaaatct acgcgcgtcg gcctgattgc
    18301 ctgcggttat gcggacggtt atccgcgccg cgccccaagc aattcccccg tcgctgtcga
    18361 cggcaaattg acccgggtca tcggcagggt ctctatggat atgatgacca tcgagctgga
    18421 tgcttcgcaa gaaggtttgg gacacgaggt cgaactgtgg ggcgatacgg tcaacatcaa
    18481 taccgttgcc gaagcggccg gaaccatccc ttacgaattg atgtgcaata tcaaacgtgc
    18541 aaaattcact tatatcgagt aatcgagtcc aaacaaaaat gccgtctgaa gcctttcaga
    18601 cggcataaat gcgttttgac aaaatcagta tagtggatta acaaaaatca ggacaaggcg
    18661 acgaagctgc agacagtaca aatcgtacgg caaggcgagg caacgccgta ctggtttaaa
    18721 tttaatccat tataaaagac attgccaaat agaaaatact gataatgccc acaatctgaa
    18781 accccatcct tcccgcaaaa acggaaaatc aaaatcagaa acctaaaatt tcgtcattcc
    18841 cgcgcaggcg ggaatccggt tttttgagtt tccgtcattt ctgataaatt ctcgtcgttt
    18901 ttcatttcta gattcccgcc tgcgcgggaa tgacaaaatt aaagtttcag aatttatttg
    18961 aacaacaaac tagattcccg ctttcgcggg gatgccggcg gataggtttt gttgtttcgg
    19021 ataaattcct gttgtttttt ggtttccaga ttcccacctt taaaagaatg acgtaagaaa
    19081 gcagaaataa ggacacaggg attt
//
LOCUS       DAXHSJ010000024        18696 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000024 DAXHSJ010000000
VERSION     DAXHSJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.024"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..1770
                     /locus_tag="ACFM8U_000613"
     CDS             <1..1770
                     /locus_tag="ACFM8U_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6333896.1"
                     /translation="IKTTIHNETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVL
                     SAPGGYIVDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEG
                     MTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATT
                     GVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAA
                     VTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNL
                     EANILAALVNTAHGEAASKIKGLDQHYVAHKIAHAVAGCAAAAANKGKCQDGAIGAAV
                     GEIVGEALVKNTDFSDMTPEQLDLEVKKITAYAKLAAGTVAGVTGGDVNTAAQTAQNA
                     VENNAVKAVVTAAKVVYKVARKGLKNGKINVRDLKQTLKDEGYNLADNLTTLFDETLD
                     WNDAKAVIDIVVGTELNRANKGEAAQKVKEVLEKNRPYIPNKGAVPNMSTYMKNNPFG
                     KQLAQISEKTTLPTQQGQSVFLVKRNQGLLKTGDRFYLDGQHKNHLEVFDKNGNFKFV
                     LNMDGSLNQMKTGAAKGRKLNLK"
     gene            1779..2156
                     /locus_tag="ACFM8U_000614"
     CDS             1779..2156
                     /locus_tag="ACFM8U_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333897.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            2434..2808
                     /locus_tag="ACFM8U_000615"
     CDS             2434..2808
                     /locus_tag="ACFM8U_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333898.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            2894..4648
                     /locus_tag="ACFM8U_000616"
     CDS             2894..4648
                     /locus_tag="ACFM8U_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6333899.1"
                     /translation="MPSEKLTIFQTAFVMQVNIQIPYILPRCVRAEDTPYACYLKQLQ
                     VTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTSGAGTGAVLGLNGAAAAA
                     TDAAFASLASQASVSFINNKGDVGKTLKELGRSSTVKNLVVAAATAGVADKIGASALN
                     NVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIK
                     QLDQHYITHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIVGEALVKNTDFSGMTASE
                     IEKAKANITAYAKLVAGTASAVVGGDVNTAANAAQIAVENNTLYPRCVGAKCDEFQKE
                     QQKWIRENPEEYREVLLLQTGFIPIIGDIQSFVQAQTAADHLFALLGVVPGIGESIQA
                     YKVAKAAKNLQGMKKALDKAATVATAQGYVSKTKIKIGQTELRVTAATDKQLLKAIGE
                     GRDTTGKMTEQLFDSLAKQNGFRVLSGGKYGGNNGFDHVWQAADGSVVLIVESKQIRN
                     GTVQLNPNGAGGYTQMSEDWIKQVANSLPDGSPAKAAVFKAEREGKLKTAIAGVDRQT
                     GKAVILSVKVPSKTNIRR"
     gene            4651..5586
                     /locus_tag="ACFM8U_000617"
     CDS             4651..5586
                     /locus_tag="ACFM8U_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HFC6333900.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPEAIDYVDERKGVP
                     LAAMRHILMYTEAAASHAYLFEHDLNKFKQYAYVAGKLGVLLSVNAEDPEPFFFPCDM
                     LNIQNPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLVHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPAAEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            6150..6353
                     /locus_tag="ACFM8U_000618"
     CDS             6150..6353
                     /locus_tag="ACFM8U_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333901.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
     gene            6917..7375
                     /locus_tag="ACFM8U_000619"
     CDS             6917..7375
                     /locus_tag="ACFM8U_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333902.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            7374..7508
                     /locus_tag="ACFM8U_000620"
                     /pseudo
     CDS             7374..7508
                     /locus_tag="ACFM8U_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            7587..7868
                     /locus_tag="ACFM8U_000621"
     CDS             7587..7868
                     /locus_tag="ACFM8U_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6333903.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(7889..9115)
                     /locus_tag="ACFM8U_000622"
     CDS             complement(7889..9115)
                     /locus_tag="ACFM8U_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC6333904.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            9270..9456
                     /locus_tag="ACFM8U_000623"
                     /pseudo
     CDS             9270..9456
                     /locus_tag="ACFM8U_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            9660..10319
                     /gene="queC"
                     /locus_tag="ACFM8U_000624"
     CDS             9660..10319
                     /gene="queC"
                     /locus_tag="ACFM8U_000624"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC6333905.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            10347..10865
                     /locus_tag="ACFM8U_000625"
     CDS             10347..10865
                     /locus_tag="ACFM8U_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC6333906.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            10872..11294
                     /gene="queD"
                     /locus_tag="ACFM8U_000626"
     CDS             10872..11294
                     /gene="queD"
                     /locus_tag="ACFM8U_000626"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC6333907.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            11563..11934
                     /locus_tag="ACFM8U_000627"
     CDS             11563..11934
                     /locus_tag="ACFM8U_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC6333908.1"
                     /translation="MKLLFIPLVLFVAAEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            11931..12569
                     /locus_tag="ACFM8U_000628"
     CDS             11931..12569
                     /locus_tag="ACFM8U_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC6333909.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGIPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQNGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            12784..12915
                     /locus_tag="ACFM8U_000629"
     CDS             12784..12915
                     /locus_tag="ACFM8U_000629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333910.1"
                     /translation="MAGLGKTKCRLKQNAVSDGIFLLPPKGKTAPAMDTEAVWIWSE"
     gene            complement(12904..12980)
                     /locus_tag="ACFM8U_000630"
     tRNA            complement(12904..12980)
                     /locus_tag="ACFM8U_000630"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12944..12946),aa:Pro,seq:cgg)
     gene            complement(13061..14146)
                     /gene="nagZ"
                     /locus_tag="ACFM8U_000631"
     CDS             complement(13061..14146)
                     /gene="nagZ"
                     /locus_tag="ACFM8U_000631"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC6333911.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGVILFRRNFQ
                     NIVQLKELTAEIKALRTPGLIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDSESASA
                     AETAARQIGWVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHRNPEAVTRLALAL
                     QKGLEKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELETADLIPFRGMSREGMAA
                     VMPAHVVYPQVDAKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     LSFEAGCDIILVCNRPDLVDELRDGFTIPDNPDLAGRWQYMANTLGSAAAQAVMQTTD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(14202..15722)
                     /locus_tag="ACFM8U_000632"
     CDS             complement(14202..15722)
                     /locus_tag="ACFM8U_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC6333912.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETTVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVDPTLIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAGAAAAGYLVLGITKSALLGFGTTGIVLAVLIFMLKDKKRTIA"
     gene            complement(15924..17423)
                     /locus_tag="ACFM8U_000633"
     CDS             complement(15924..17423)
                     /locus_tag="ACFM8U_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC6333913.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLRAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            complement(17561..18190)
                     /gene="nth"
                     /locus_tag="ACFM8U_000634"
     CDS             complement(17561..18190)
                     /gene="nth"
                     /locus_tag="ACFM8U_000634"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC6333914.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(18236..18655)
                     /locus_tag="ACFM8U_000635"
     CDS             complement(18236..18655)
                     /locus_tag="ACFM8U_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC6333915.1"
                     /translation="MMPAYAYAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYG
                     ALLCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALP
                     LAAGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         5014 a         4482 c         4823 g         4377 t
ORIGIN      
        1 atcaaaacca cgatccacaa cgaaaccgtg agcagcagca aatctgctct atggcaaaaa
       61 caggcaggac ggggcagtaa catcgaaacc ttgcaattgc cgagtttcac cggtcccgtt
      121 gcgcccgtac tgtccgcacc cggcggttac attgtcgata ttcccaaagg caatctgaaa
      181 acccaaatcg aaaccctcac caaacagccc gagtatgctt atttaaaaca gcttcaagtt
      241 gcgaaaaaca tcaactggaa tcaggtgcag cttgcttacg ataaatggga ctacaaacag
      301 gagggcatga cacccgcagc agcagctgtc gtcgttatcg tcgtaaccgt attgacctac
      361 ggcgcactgt ccgccccggc agccgccgga acggcgggcg cggcaggcgc aggagcggga
      421 ggagccgcag caggaacggc agccggaact ggagtagcag caggaacggc agccacaacc
      481 ggagtagcag caggcacatc agctgcagct atcaccacag ccgcaggcaa agccgcactg
      541 gccagtctcg ccagccaagc cgcagtttcc ctcatcaaca acaaaggaga cataaaccat
      601 accctgaaag aactgggcaa aagcagcacc gtcagacagg ccgccaccgc cgccgtaacc
      661 gcaggcgtac tgcagggcat aagcgggctg aacacccaag cagccgaagc cgtcagcaaa
      721 cattttcaca gtcccgcagc aggcaaactg accgctaacc tgatcaacag caccgctgcc
      781 gcaagtgtcc ataccgccat caacggcggc agcctgaaag acaatctgga ggcgaatatc
      841 cttgcggctt tggtaaatac cgcgcatggg gaggcggcga gtaagatcaa aggactggat
      901 cagcactatg tcgcccacaa aatcgctcat gccgtagcgg gctgtgcggc tgcagcggcg
      961 aataagggca aatgtcagga cggcgcgatc ggtgcggctg tgggtgagat tgtcggggag
     1021 gctttggtta aaaataccga ttttagcgat atgaccccgg aacaattaga tctggaagtt
     1081 aagaaaatta ccgcctatgc caaacttgcg gcaggtacag ttgcaggcgt aacgggagga
     1141 gatgtcaata ctgctgcaca aaccgcacaa aacgcggtag aaaataatgc ggttaaagct
     1201 gttgtaactg ctgcaaaagt ggtttataag gtagccagaa aaggattaaa aaacgggaaa
     1261 atcaacgtta gagatttaaa acagacgttg aaagacgaag gttataattt agccgacaac
     1321 ctgaccacct tattcgacga aacattggat tggaacgatg ccaaagccgt tattgatatt
     1381 gtcgtcggaa cagagctgaa tcgcgctaat aaaggggaag cggcacaaaa ggtcaaggaa
     1441 gttttagaaa aaaatcgtcc ttatatccct aataaaggtg ctgtaccgaa tatgagtaca
     1501 tacatgaaaa ataatccttt tggaaaacag ctggctcaaa tttcagaaaa gacaacgctt
     1561 ccgacgcagc aagggcagtc tgtcttcttg gtaaaaagaa accaagggtt attaaaaacc
     1621 ggtgataggt tttatttaga tggccaacat aaaaatcatt tagaggtttt tgataaaaat
     1681 gggaacttta agtttgttct aaatatggat ggttcgctta accaaatgaa aactggggca
     1741 gcaaaaggtc gtaaattaaa cttaaaatag gaaattttat ggaaacattg aatgatataa
     1801 aaaaaatctt gattaatgtg gggctttatc aagggtttga tttgacagat ccaaaagtat
     1861 cagaagaagt taatcatgaa acagctaata tgaaatggat taaagattat acttcagacg
     1921 ggaattggga taatgaattt aaggaggatt taaaaaactt tttagattat atggaagtat
     1981 gccaattagc cctaaacgat aaaaatttca aaattgccag taattcttta tttatggcta
     2041 tgatttacgc aggtaatcta tctcttatat ttgattcaat aaaaactgat atatcaacat
     2101 tattgagtgc tgagtataaa aagaatagtt tttcatggcc atctcttgat gaatagaaag
     2161 caagttgtag cctgcatgaa atctaaaacc catgcataag gtgtgggctt cagtatacgc
     2221 gttccatgat ttacggccat atgccgtctg aaaagctcaa ttttttcaga cggcatttgt
     2281 tatgaaagta aatatttaga ttccctgtat actgtttaga ctcgtgtgtg ctgagtaagc
     2341 tgtagtctgc atgaaaccta aaactcgctc aaaattaagc taagacatta gcagggcaag
     2401 ggcgaaaatt gaatcttaaa taaggtgatt cagatgaaaa attttaatgt agtaaaagaa
     2461 agtttaagag agttaggaat taaacaagga tttgatcttt atgagaaagc cacaactgaa
     2521 aaattgaata gtgaagatcc tcttgactta caatggcttt ctaactattc atctgattgg
     2581 aatgatgaat tagaagaaga ctttgattct ttttttcagc atatgaagga atatcaatat
     2641 gctattgaca atgaagacat taaatctgca tgtagttcac tatgtgaagc tatgctctat
     2701 gttggtaata ttaaaaattt ttttgagttt ctcaaaagcg atatgattag actgttgaga
     2761 ggtgaaagta aaacaacaga ctttcaatgg ccgcaatttg atgaatagca gcaagctgta
     2821 gcctgcatga aacctaaaat ccatgcgtaa ggtgtgtgct tcagcacgca cgcgttccat
     2881 gatttacggc tcaatgccgt ctgaaaagct cacaattttt cagacggcat ttgttatgca
     2941 agtaaatatt cagattccct atatactgcc cagatgcgtg cgtgctgaag acacccccta
     3001 cgcttgctat ttgaaacagc tccaagtcac caaagacgtc aactggaacc aggtacaact
     3061 ggcgtacgac aaatgggact ataaacagga aggcttaacc ggagccggag cagcgattat
     3121 cgcactggcc gttaccgtgg tcacctcagg cgcaggaacc ggagccgtat tgggattaaa
     3181 cggtgcggcc gccgccgcaa ccgatgcagc atttgcctct ttggccagcc aggcttccgt
     3241 atcgttcatc aacaacaaag gcgatgtcgg caaaaccctg aaagagctgg gcagaagcag
     3301 cacggtgaaa aatctggtgg ttgccgccgc taccgcaggc gtagccgaca aaatcggcgc
     3361 ttcggcattg aacaatgtca gcgataagca gtggatcaac aacctgaccg tcaacctggc
     3421 caatgcgggc agtgccgcac tgattaatac cgctgtcaac ggcggcagcc tgaaagacaa
     3481 tctggaagcg aatatccttg cggctttggt gaatactgcg catggagagg cagccagtaa
     3541 aatcaaacag ttggatcagc actacattac ccacaagata gcccatgcca tagcgggctg
     3601 tgcggcagcg gcggcgaata agggcaagtg tcaggatggt gcgataggcg ctgcagtcgg
     3661 tgagattgtt ggtgaggctt tggttaagaa taccgatttc agcggtatga ctgcttctga
     3721 aattgaaaaa gctaaagcga atattactgc gtatgcaaaa ctggttgccg gcactgcgtc
     3781 tgccgttgta ggcggggatg tgaatacagc ggcgaatgcg gcacagatag cggtggagaa
     3841 taatactttg tatcctagat gcgttggtgc aaagtgtgat gaatttcaaa aggaacaaca
     3901 aaaatggata cgtgaaaatc ctgaagaata tcgagaagtt ttgcttcttc agacaggatt
     3961 tattccaatt atcggtgata tacagagttt tgtacaagca cagaccgctg ccgatcacct
     4021 gtttgctttg ctgggtgtgg ttccgggtat cggtgaatcg atacaggcct ataaagtagc
     4081 gaaagcggca aaaaatttac aaggcatgaa aaaagccttg gacaaggcag caaccgttgc
     4141 cactgcacag ggctatgtca gtaaaaccaa aatcaaaatc ggtcaaactg aattaagggt
     4201 tactgcagca actgacaaac aattgctgaa agctattggc gaaggaaggg acacgacagg
     4261 taaaatgacc gagcagttat ttgactcttt agctaaacaa aatggcttca gagtgctttc
     4321 gggcggcaaa tacggcggaa ataacggttt tgatcatgta tggcaggctg ccgatggtag
     4381 tgtcgttttg attgtagaaa gtaagcagat taggaacggt acggtacagc tgaatccgaa
     4441 tggtgcgggt ggatatacgc agatgagtga ggattggatt aaacaagttg caaatagctt
     4501 acctgatggt agtcctgcta aagctgctgt cttcaaagca gagcgggaag gcaaattaaa
     4561 aacagcaata gcaggcgttg atcgtcaaac aggtaaggcc gttattcttt ctgtcaaagt
     4621 tccttctaaa accaatataa ggagataaca atggggcaca atatgatgac cacccaaaaa
     4681 tggtatgagc atattactaa tgtaatcata ggcaatactg ctaatttcaa tagcggttgc
     4741 cctgaagcta tagattatgt agatgaaaga aaaggtgtgc cgcttgcagc gatgagacat
     4801 attttaatgt atactgaagc tgcggcttcc catgcctatc tatttgaaca tgatcttaat
     4861 aaattcaagc aatatgctta tgttgcagga aagctggggg ttttgctgag tgtaaatgct
     4921 gaagaccccg aacccttctt ctttccctgt gacatgctca acattcaaaa tccgatgttt
     4981 ctgatgctga tgagcgacag cccacagctg cgcgagtttc tggtgcgcaa tatcgacaac
     5041 atcgccaacg atacagaagc cttcgtaaac cgatacgacc tcaaccgtca tatgatttac
     5101 aatactctgc tgatggtgga gggtaagcag cttgatcggt tgaaacaacg tagcgagaaa
     5161 gtcttggtgc accccacccc tagcaaatgg ctgcaaaagc ggttgtacga ttaccgcttc
     5221 ttcctcgctt tcgccgaaca ggatgccgag gcgatgaaag ccgccttaga gccgcttttc
     5281 gataaaaaaa ccgcgcgtat ggctgccaaa gaaacattgt cctatttcga tttctacctg
     5341 cagccgcaaa tcgttaccta cgccaaaatc gcatccatgc acggtttcga tttgggcata
     5401 gatcaagaaa tcgcaccgag ggatttgatt gtttacgatc cgctgccggc agccgaatac
     5461 caggacatct tcgattttat gaaacagtac gacttgtctt acccgtatga atatctgcag
     5521 gattggatag attactatac gttcaaaacc gataagctgg tatttggtaa cgcgaagcga
     5581 gagtgagccg taaaactctg agctcctgtt ttatagatta caactttagg ccgtcttaaa
     5641 gctgaaagat tttcgaaagc tataaattga agcccttcca cagtacatag atctgtgttg
     5701 tggcggggct ttaccacgct gattgccgga gaagaactca acctgctggc aaaacaaggc
     5761 atgagatctt tgcaataaca tgagttgaga cctttgcaaa aaagcccttc cccgacatcc
     5821 gaaacccaaa cacaggattt cggctgtttt cgtaccaaat acctcctaat tttacccaaa
     5881 tatcccctta atcctccccg gatacccgat aatcaggcat ccgggctgcc ttttaggcgg
     5941 cagcgggcgc atttagcctg ttggccgctt tcaacaggtt caaacacatc gccttcagat
     6001 ggctttgcgc actcactttg tcatttccaa tgttccaagt acacctacta cgataagagg
     6061 aagagagctt gaaggaaaac ttattttaga agttcctgct caggtcaatc caattccaca
     6121 atctgtatta agggcggcac aagaagaaaa tgttatcatt agagatacaa caggaaggat
     6181 ttacaatagg tggtggttta gtattaggtg gttgtgcagg tgcacatctt gcaagaaaag
     6241 aaccattgat actaacaggg aaaacagggg caggtgcgtc agcaattgca aatgcaagca
     6301 ttggatatca atggactgtc aatttgtcag agccaaaaga aggagctaaa taataatgca
     6361 ttcccactat atatttggta ttttgatgat ttcatatgtt ttcgcaatgt tatttaattt
     6421 tataatatca tataaaatat ttaaagaaga aaaattaatt aatggttttt ttgattttct
     6481 acttaaatca agctacctta actttaaata tttcaatata ttatttggaa aagaaaaaat
     6541 ctcaaatatt ttttatttga aattattaag aattaatctg gcgttggggg tttttatctt
     6601 atccttaata attataaata ttttttgttt gtagtaaaaa tatggtacag atatgtacag
     6661 ctagctttgt ttcagtaagg tataactgta tataatactc agatttttca cgttgggcta
     6721 tacatggaaa tatatctgtg attaaagatg ttaatggtaa gtatcgatta gcacctgaaa
     6781 agcatgattt taaaatgcat tcctttgggg ggagaaaaaa gtaatgtaaa aacaatattt
     6841 agaaatatgg aaactataat tggtagccca gggtaagggg tacctttcag gattgaattt
     6901 aaaggagagg taaatattgt taactaagtt gaaaattttg ctatttttgt tcttatttgt
     6961 ttttgtattg gctattaatt tgcttttctt cttttttagt tcggatatcg agagtttcgg
     7021 gaactatcag tttgaatatg tttacgataa aggttggcct gctaattata ttttagtcat
     7081 gaaagatgga aatgaaggga attttgataa aataatatcc ggattggttt tagaatatta
     7141 taaggaggat gataacattt atttttctta tattgacggg caaggatttg cttcagactc
     7201 ttgctattac aaaccggaaa ttttatatgg aaaaattatt ttaaataaaa atcatatcat
     7261 taatattaat agtatggaaa aaaataattt tctttcagaa gataaaataa tgaagggaac
     7321 aagaaattgg ctagcagacc ctaaaaataa atgtaatata cagactctag actaaacgcg
     7381 tcttgcgaaa atacaacgga atcgatcgta aatctttccc gctgttcttg aaagaatgcg
     7441 aatttcgatt taacttcggc acaccgtctc aacagcttaa aatcctgcgg gattggtgtg
     7501 ggatttaggg ctaatctagt acagcccctt gttttttcga tacggaaccg gatagaggaa
     7561 aaatcgaaca ttgcgcctgc cttgctatga ttcacgaaga aatctccgcc atgcctatgg
     7621 gctatgaaac cttgatcggc gatatgggca gcgcactgtc aggcggacaa aaacaacgca
     7681 tcgtattggc gcgggcctta tattgcgaac cgaaaatcct atttttagat gcagcgacca
     7741 gccatttgga tattgccaat gaaaaagcag tcaatgcaaa cttgaatggc ttgtctatca
     7801 taaaaattat ggcggcacac agaaaggaaa cggtggaatc agcagatagg aaaatgtctt
     7861 taggataaaa atacagtttc aaaaatactc aagattgctg ctgttttttc gcctgagcgt
     7921 caaactctgc cagcgtcatg ttcaaagtct gcaaacacgg catcattact gcatcgacag
     7981 cttggttcac atgatccctt tccacaggca acggacggta aacgaagagc ttgaagagtt
     8041 cgttcaactc aatcgaatcc gcccccgttt tcaacaccca accctgtctg ccggaataga
     8101 tgtagccgtg ccgcgccagc ttttccaaaa gctcgcccaa ctcgtcgtag cccatattga
     8161 tatgccgtct gaactcctga acaggcaagg ctttgccttc tttttgcgcc gcatccagaa
     8221 gcagcaggat tttcaacacg tcgtcaaacc gtccgcgcga gtcgaagccc ctgcggaacg
     8281 cttctccctg ccagtaggag agtgaagaag tcagcaccgc gccgcccaag accagcgtcc
     8341 acaacaggtt cagccacaac agaaaaaacg gcacggcggc aaacgcgccg taaatcgagc
     8401 ggtagccgtc gaaattgccc atataccaag tgaagaggga gcgcgcggtt tccagacaaa
     8461 acgctgttgc caaagccccg acaaacgcct gccgcgcggg aacgaagcgg tttggcacga
     8521 agcggtacag cccccacagc aaaagcgtca tgaaggtcag cgtcgccgcc gttcgcaacg
     8581 cgcccgacca ctgcggcgca cctgaggcaa gcgcggcatc ctgtaccgag ccgaccataa
     8641 aggaaatgcc cacgcccaaa gacagcggcc cgaacgtcag taaagcccaa tagacgagaa
     8701 actgcatcat ccacggacgc tgggaattga cccgccagat gcggttgaac gtattgtcta
     8761 tcgtccgaat cagcatcagc gaggtaacga ccagcatcac gctgccgatt gccgtcagcc
     8821 ggttcgcctg ctcgcggaac gcattgatat agtcgaacac catgtccgcg ccctgcggca
     8881 caatggtttg gttgacgaag gagacgaacg aatccgacca gcggtcgaac acggggaaaa
     8941 tcgaagcgac cgccaccatc acggtcagca cggggacgag tgccagcagc gtcgtaaacg
     9001 tcatgcttgc cgccgcctgc ggtacgcgtt cttcatcaaa gcggcggacg acgaaccatg
     9061 caaacgcaca gattttattg tctgccaaac cttgcaaacg ttgtaaaaag gtcataattt
     9121 cttgcccggt cagtaagttg ggcattgatg cccgatgtta tagccaattt tgccgtcagg
     9181 aacaaatgcc tgaactgcgg ctgtttcaga cggcatcgga acaactgtta tgccgtctga
     9241 agaccgaacc attttaacgg aatccgccca tgaacccaaa tccccctcaa aatcctcgtc
     9301 ctctactatt tccaaaacgg cagcacccgc aatcccgccc gccgaatcgc tcgcggcatc
     9361 gacagcgttg aaggttgcga agccgtattg cgcaccgtcc ccaaagtctc cgccgtctgc
     9421 gaagccgtca aaaaagatat tcccgacagc ggctcccgtc ctgaccgccg aagaaaacaa
     9481 tatcgccttc gcacaaagca aacgcttggc ggaactcgcc atcaagtcgg cataagccgc
     9541 gtgttcagac ggcatggcgt tcagatgccg tctgaacgcg tttgcctgta taatccgcgt
     9601 ctttactatc caacttcgcg gttcgaaaac ctcccgcgtc accaaaacta ggattcgata
     9661 tgtcaaacca aaaagccttg gtcatctttt cgggcggtca ggattcgacc acctgcctga
     9721 ttcaggcaat ccaaacctac gggcgcgaaa acgtccaagc cattactttc caatacgggc
     9781 aacgccatgc cgtcgagctg gaacgtgccc gctggattgc gcaggatttg ggcgtcaaac
     9841 aaaccgtact cgacttgagc ctgatgcggc agattacgca caatgccctg atggacgaca
     9901 ccgccgccat cgaaactgcc gaaaacggcg ttccgaatac ctttgtagac ggccgcaacg
     9961 cgcttttcct gctctatgcc gcgatttacg ccaaagggca ggggatacgg cacatcatcg
    10021 cgggcgtgtg cgaaaccgac ttctccggct atcccgactg ccgcgacgtg tttgtcaaat
    10081 cgatgaacgt tacccttaat ttggcgatgg actatgattt tcaaatccac acgccgctga
    10141 tgtatctgac caaggcgcaa acttgggcgt tggcggacga aatgggcgtg ctggactata
    10201 tccgcgagca aacccacacc tgctataacg gcatcgtcgg cggctgccgc gaatgcccga
    10261 gctgtatctt gcgcgaacgc gggctggcgg aatatctgga aagtaaaaag gccgtctgaa
    10321 cacgcgcaaa ccataaggaa tacgatatgc ccaagctcca tatgttttac ctcggcggca
    10381 atgccggcag gtcgaatatc gaagtgcacg acatccaatt tgctgtgtgc gacgactacc
    10441 gtgaagccgt ccccgcactc aaagccgcgt ggttcggcga tgcggacaaa atccacatcg
    10501 acggctggca ggttgtcgaa tgggcggacg gttacgacat cgccgtatcc gaaacgccca
    10561 aaacgaaaat gccgtctgaa aacgccccgc gcttgtattt tgccaatgtc ggcggctatc
    10621 gcgcagggca gctcgccgaa gcgcacgctt ttgggctgtt cgccgccgcc acgcctgccg
    10681 aagccaaaca aaaagccctg caaaccctgt tgaccgacag ctatgttcag caacataaag
    10741 acaacttaaa agacgtggac aacctgcttg cgctcgaccg catcggcaat ttccatatcc
    10801 gcctgacccc gaatccgcac ggcaagcctg ccgaaatcgg ctttcagggc tatttgccca
    10861 tttgagaacc catgaaaatc accaaaatct tcaccttcga ctcctcgcat atgctcgacg
    10921 ggcatgacgg caaatgccaa aacctgcacg ggcataccta caaactcgaa atcaccgttt
    10981 cagacgaccc cgtcagaggc gggccgaaag acggtatggt gatggacttt accgacttga
    11041 aagccattgt caaacaacac attaccgacc ccttcgacca cgccttcatc taccacggcg
    11101 gcaacgggcg cgaatctcaa atcgccgcac tcttggaagg ctggaacatg aaaaccctgc
    11161 gcctgccctg ccgcaccact gccgaaaata tggcggtcga aatgtacgac cgtctgaaaa
    11221 acgcggggct gaacgtgtgc cgcgtcaaat tgtgggaaac gccgacatcg tgtgcggagt
    11281 atgaagggga gtagggaata tcttgaacgt atcgatatag taaattccaa taagacatgc
    11341 ccaaccgcgt cattcccgcg caggcgggaa tccagacctt gatttatcag gaatatttaa
    11401 aaattgcagc aattccaact ctctggattc ccgcctgcgc ggaaaggacg gtttagagcg
    11461 tccttatttg aatttaccgt aaaacggttt tttctcctgt acggattccc cgttttttca
    11521 gacgaccttc catatcaaat acacccatta aaaggaatac ccatgaaact cctcttcatc
    11581 cccctagtcc tcttcgtcgc cgccgaacat ttctacatcg cctggcttga aatgacgcag
    11641 attcccagcg aaaaagcggc ggaaacgttc aagctgcctt atgaatttat ggaacaaaat
    11701 cgcgtgcaga ccctgttcgg caaccaaggg ctgtataacg gctttctcgg catcgggctg
    11761 gtgtggtcgc ggtttgccgc gccggataac gcggtgtacg gcgcgacggt actgtttctc
    11821 ggcttcgtcc tgattgccgc cgcgtggggc gcgttctctt ccggcaacaa aggcatactc
    11881 gtcaaacaag gtttgcccgc atttttggca gcggcggcgg tgttggcggt atgaaaaaaa
    11941 tcaatgttgc ccccgaaaat ccccaatacc gcatcgtcga aatctttgaa agcctgcaag
    12001 gcgaaggctg gaacacgggc atacccgccg ttttcgtccg cttgggcaaa tgcaacctcg
    12061 cctgcggctg gtgtgatacc gattatttga cattcggcat gatgagcctg tccgacatct
    12121 taggctgtct gaaaacctat gccgcccgca acatcatcat caccggcggc gaaccgacca
    12181 tacagccgca tctcgatatg ctgctggaca tgctcaaggc ggaaggttat ttcctctgta
    12241 tcgaaaccaa cggactcaat cccgcgccgc cgcaaatcga ctatgtcgcc accagcccca
    12301 aagcctgcta tgccgccaaa tacgaaaaaa gctgtatcga aacagccgac gaagtgcgga
    12361 ttgttgccga cggcgatgtc gttgcgttct gtgaaaatat ggaacgcaaa atccgtgcgc
    12421 atcattacta cctttcgccc tgcgaacaaa acggtgcaat gaacatctac gacaccatcc
    12481 gccaaatcgg tattctgaac agccgccccg acgcgccggt gcattggcag ttgagcgtgc
    12541 agacgcacaa atgggcggga atagagtagt ttaagcagtg taactcaaag ggacggcgta
    12601 cggttttacc gatgtttgac atacggggaa agtgtgccgc ttctgcgtgg aaatgccggc
    12661 atttccaccg cccaatcagg acggagcctt actgaataag atgctgccgt tgggtacaag
    12721 ctcggcttcc taaattccga tggtcttttg aaccttgccg ataccctttg tcggcgcacg
    12781 caaatggcag ggttggggaa aacgaaatgc cgtctgaaac agaatgctgt ttcagacggc
    12841 atttttctgt tgccgccaaa aggaaaaacc gccccggcaa tggataccga ggcggtttgg
    12901 atttggtcgg aatgagagga ttcgaacctc cgaccccttc gtcccgaacg aagtgcgcta
    12961 ccgggctgcg ctacattccg aatgaattgg aacgggatta taacgtaaaa agagcggcat
    13021 gcctataccg ttttgccttt ttgccgcgtg tcgggaggat ttaaaaggcc tctccgactt
    13081 tcacgccgcc cgccgtgtct tgcggcgagg ccagaccggc aacaaaggct tgcgccgctt
    13141 ggaaatccgt tgtctgcatc acggcttgcg cggcggcact gccgagcgtg ttggccatgt
    13201 attgccaacg tccggccaaa tccgggttgt cgggaatggt gaaaccgtcg cgcaattcat
    13261 cgaccaagtc gggacggttg cagaccaata tgatgtcgca acctgcttca aacgacagtc
    13321 gggcgcgttc tttgataccg ccagccccgc acgcgccttc catggtcaaa tcgtcagaga
    13381 aaattacgcc tttgaatccg atatcgcggc gcaaaatttg tttgagccag atttcggaaa
    13441 accctgcggg cttggcatcc acttgcggat aaacgacgtg ggcaggcatg accgctgcca
    13501 taccttcccg gctcatgccg cggaagggga taaggtcggc ggtttcgagt tcggacaggc
    13561 tgcgcctgtc ttccggcaag accagatggc tgtctccttc gacaaatccg tgtccgggaa
    13621 aatgtttgcc gcatgatttc atgccgcctt tttccaaacc tttttgaagg gcgagggcaa
    13681 ggcgggttac ggcttcgggg ttacggtgga aactgcggtt gccgatgacg gggcagtttc
    13741 cccagtccaa atccaagacg ggagtgaagg acaagtcgat gccgcaggcg gaaagttcgg
    13801 ttgccaaaac ccagcctatc tgccgggctg cggtttcggc ggcagatgcg ctttcgctgt
    13861 cccaaatttg ccctaagacg ttcattgcgg gcaagcgggt aaagccttcg ataaaccgtt
    13921 gaaccctgcc gccttcgtga tcgacggcaa taataagccc gggtgtgcgc agggctttga
    13981 tttcggcggt aagttctttg agctgtacga tattttggaa gttgcggcga aacaggatga
    14041 ctccgccgac ggcgggatcg agaaggcgtt gcttttcctc ttcggtcaga cggaaggcgg
    14101 caatgtcggc cattacgggg ccgcgcggaa tatggggaac agtcatttcg gtttgctcca
    14161 aaaagcttca gacggcatat gccgtctgaa aaggggggaa atcaggcgat ggtgcgtttt
    14221 ttatctttca acatgaaaat cagcaccgcc aatacaatgc cggtcgtgcc aaagcccaac
    14281 agcgcggatt tggttatgcc caaaacaaga taaccggccg ctgctgcacc cgctacggtc
    14341 agggcatagg gcagttgcga ggtaacgtgg tcgatatggt tgcagcgcgc gccggtggaa
    14401 gacaggatgg tcgtgtcgga gatgggcgaa cagtggtcgc cgcataccgc cccagccatt
    14461 actgcggaca tgcacgggat aatcagcgtg ggatcgactt tgaccgccat tgccgcggca
    14521 atcggcagca tgatgccgaa tgttccccaa cttgtgccgg tggcaaacgc cattacgctg
    14581 gcgagcagga agaggatgac gggcaggaag ccgggatgga tgttgcccgc aaccagcgtg
    14641 gagaggtaat cgccggtgtg catttcgccg acaaccgtac tgatgagcca agcgaggatt
    14701 aaaatggcga ttgcgccgaa catagatttc gcaccctgcc aaacggcttt gggatagtcg
    14761 gcggttttaa tcgtgccgag cgtgcagaga acgacggcaa gcacgccgca agtgccgccg
    14821 aataccagcg aagtgtttac gtcggtattt tcaaatgccc ccaaaatgct gaaggtttcg
    14881 cttgcctgcg cgccggtgta gatcatggcg gaaaccgttg aggcgattaa ggccaaaacg
    14941 ggaataatca gtgcgtaaac acgacctttg gtagcgtctg aaacggtagt ttcatcgtgg
    15001 gcttcgttca acgcggcttg ttcgaaacgt gccatcgagc cgatgtcgaa ggaaaaccat
    15061 gcgacgacga acaccataat cagggcaaac agtgcgtaat agttcatcag gctcatggcg
    15121 acaaacgtcc ccatcggcgt gtattcggtg attttgtagg taacgagcag tccggcaagc
    15181 gtggcgataa tcgacgcgcc ccagcttgaa acgggcatca gcacgcacat aggcgcggca
    15241 gtggagtcga ggatgtaggc gagtttggcg cgggaaactt taaacttgtc ggtaacgggg
    15301 cgggcaatcg caccgacggc gagactgtgg aaatagtcgt cgataaaggt tacaaacacg
    15361 aggcaggcgg tcagcatttt cgcgccgcgc cggtttttaa tgtgccgttt tgcccagtcg
    15421 gcaaacgcct gattgctgcc ggagtaggtc agcagggaag taaaaatacc caaaagtatc
    15481 aggaaaacca agatttttgg tttgcccagc gaccaatcgc cgtctgacca agccaagccg
    15541 acgaccatgt ctttcaggtg tgtcagaccg tcgacggggt tgccgccgac caaaaaggca
    15601 acgccgacca gaataccgat gcctaaagac agcagtacgc ggcgggtaat gacggcaagt
    15661 gccagtgcca aaaagggtgg cacaaccgag aaaaatgaat gtgaatagtc gatcagctgc
    15721 atggttatgg gggtattaag cgtccggatg ggagcgtatc tgtccgcctc cggtttgggt
    15781 tttgttggca aaatgggcgg aaatattttt tgtcgtaaaa aatatttgtt taaaatcaac
    15841 caactgattt ttgtaaaatg cccgttaatc ggtattgacg ggcattttat catttaaaaa
    15901 atattttggt taaattatgt gggttattgc aggtttaatg cgataaacag cgtgttgcca
    15961 cggcgcatga ccagcagggg gacgtttttg cctgccttgt ccatagcttt gcggaaaccg
    16021 gcttcgtcat tgacggggac ttgcccgacg gcaagaattt cgtcgccgcg cctcaagcct
    16081 gcgcgttctg ccgcgtcgga aacccgtacg acgacgaggt gtccgccgct gctgtcggta
    16141 tgtgtctgaa gggtaatgcc tgcggattcg accgagaacg taccggattg ctgttcggtg
    16201 tagggggctt catctgtttt ggatgatgcg ccgatatgct cggcggcgtt gcccagcttg
    16261 actttgattg tgatttcttc gcctttgcgc catacgccga ggctgacttc ttttcccggc
    16321 gtaatggcgc cgaccataac gggaaggtcg ccggaagaac gtatttctcc gccgtcgagg
    16381 ctgaggacga tgtcgcccgc ccgcaggccg gcacgttctg cggggctgcc gggcaggatt
    16441 ttggcaatca gtgcgccgcc ggctttgtcc aaaccgaacg attgtgccaa accgtaggat
    16501 acttcttgaa taatcacgcc cagttgtccg cgttggactt tgccggtgtt tttcagctgt
    16561 tcggcgacat tcatggcaac gtcaatcggg atggcgaagg aaatgcccat gaatccgccg
    16621 ctgcggctgt atatttgcga gttgatgccg acgacctgtc cttttaagtt gaacagcggg
    16681 ccgccggagt tgcccggatt gatggcaacg tcggtttgga tgaagggtgt gtagctttcg
    16741 ttgggcaggc ttctgccttt ggcggacacg atgccggcgg tcacgctgtt gtcgaagccg
    16801 aagggcgcgc cgatggcggc gacccattcg cccggtttca aatctttggg attgccgatt
    16861 ttgacgacgg gcagctcttc cgttgcgtcg attttcagaa gggcgacatc ggattggaca
    16921 tccgaaccga tgagtttggc ggtatattcg cgcttgtcgt tgagcaggac tttgatactg
    16981 cccatgccgg taacgacgtg cgtattggtc agaatatagc cgtctttgct gatgatgaag
    17041 cccgaaccga agttcaatcc gccgtcatct gcttcttctt gggggatttc gggcatgttc
    17101 gggacgaggc gtttgaaaaa ttcgtagaac gggtcgctgt cggcaagcgg gtcggaatcg
    17161 gtttcggcat tgctgctgcc gttttgggtg cgcggggcgg gggctgcctg aatattgacg
    17221 actgccgggc cttcgctttg aaccagttgg acaaagtcgg gcagcagcat actgacgctg
    17281 ccgtcgtctt tggtgtgttt gatgcgttct acgaaggatg cttctttttt gtccgcaccg
    17341 aaaaagctgc cggctttgtc gcagcctgcc agcgaggcgg cacacagtgc tgccaaagcg
    17401 aggtattggt attttttgaa cacgttttgt cctttgtcgg atgccggtac cggctttaat
    17461 gccgtctgaa gcgcattttg tcggcttcag acggcatagg ttgaaattct acaacgtccg
    17521 tccgaatttt caagcgtttc attttgaagg gcggcggcag tcaggctttg gcgggatatt
    17581 cgcacaaatc gttgatgatg caggtttggc attgcggttt gagtgccttg caggtgtagc
    17641 gtccgtgcaa aatcagccag tggtgcgcgt ccatcagaaa ttctttagga atgaagcgca
    17701 tcagtttgtc ttcgacttcg cgcacatctt tcccgggtgc gattttggtt cggttggaca
    17761 cgcggaaaat atgcgtatcg accgccataa cggggtgtcc gaacgctgtg ttcaatacca
    17821 cgtttgccgt tttgcgcccc acgcccggca acgattccaa agcctcgcgg tcttccggca
    17881 cttcgccctt gtatttttcc agcaggatgc ggcaggtctg catgatgtgc ttggacttgg
    17941 ttttatacaa cccgatggtt ttcgtgtatt ccatcacgcc gtccaagccc aaatccagca
    18001 ttgcctgcgg cgtatcggca accggaaaca gcttcgccgt cgccttgttt acgccgacat
    18061 cggtagcctg cgctgaaagc agaacggcga ttaaaagctc gaaaggggag ttgaaattca
    18121 gctcggtggt cggatggggg ttggcggcgc ggaagcgttc gaaaatttct tggcggatgt
    18181 gtctgttcat ttttttgtgc agtcggtttg tgcggtcggc attataacgc acggttcagg
    18241 cggcgtaata ttgcattccc cacagaatga aggcgtaacg cgccgttttg ccgataacca
    18301 gcatcagccc gcttgtccac ggattcaacc gcagccagcc ggcggcaagc ggcagtgcgt
    18361 cgccgacgac gggcagccag gcaaacgcaa gcagccaaat accgaaacgc cgcatcagat
    18421 tcagtgtttt ttcagacggc atttttcggg agggcagcaa acgccccatc caataggaaa
    18481 ccatactgcc caatccgttg gcaaggccgg cgcacagcaa cgcgccgtat gcgtgttcgg
    18541 gaaagcggtg gacgaacagg gcaaaggcgg cttcggatgt gccgggcagg agggtggcgg
    18601 aagtgaaggc ggaaaaggcg agcgcggcgt aggcgtaggc gtaggcgggc atcattgcaa
    18661 acagtctcaa acaggcaaaa atcggcgacg gattgt
//
LOCUS       DAXHSJ010000025        16796 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000025 DAXHSJ010000000
VERSION     DAXHSJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.025"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..530)
                     /locus_tag="ACFM8U_000636"
     CDS             complement(<1..530)
                     /locus_tag="ACFM8U_000636"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFC6333916.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIRNTRDAVVNVDKLTYAG
                     NLESLTEVADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEF
                     IQTNIVGTFNLLEAARAYRQQMPSEKREAFRFHHISTDEVYGDLGGTDDLFTETAPYA
                     PSSPYSASKASSDHLVR"
     gene            complement(531..1574)
                     /gene="galE"
                     /locus_tag="ACFM8U_000637"
     CDS             complement(531..1574)
                     /gene="galE"
                     /locus_tag="ACFM8U_000637"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC6333917.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDECQQLDLYRKNIGKLDNQYKRALAFTLMRRAMIRKMPYTEDMRPGDTANPYGA
                     SKAMVERMLTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAS
                     GRLPQLSVFGGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAY
                     SVLEIIRAFEAASGLNIPYQIQPRRAGDLACSFADPSHTKQQTGWETKRGLQQMMEDS
                     WRWVSRNPNGYGD"
     gene            complement(1567..1761)
                     /locus_tag="ACFM8U_000638"
     CDS             complement(1567..1761)
                     /locus_tag="ACFM8U_000638"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6333918.1"
                     /translation="MRKVFHYEQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNA
                     VPPLLAKAIAESILKMSENE"
     gene            complement(1739..2569)
                     /locus_tag="ACFM8U_000639"
     CDS             complement(1739..2569)
                     /locus_tag="ACFM8U_000639"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6333919.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKTSRLYYRRYAQSFSL
                     "
     gene            complement(2600..4873)
                     /locus_tag="ACFM8U_000640"
     CDS             complement(2600..4873)
                     /locus_tag="ACFM8U_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC6333920.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPLDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4937..5587)
                     /locus_tag="ACFM8U_000641"
     CDS             complement(4937..5587)
                     /locus_tag="ACFM8U_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6333921.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5584..6381)
                     /locus_tag="ACFM8U_000642"
     CDS             complement(5584..6381)
                     /locus_tag="ACFM8U_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6333922.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(6381..7544)
                     /gene="ctrB"
                     /locus_tag="ACFM8U_000643"
     CDS             complement(6381..7544)
                     /gene="ctrB"
                     /locus_tag="ACFM8U_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HFC6333923.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVQEYMQSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(7559..8734)
                     /gene="ctrA"
                     /locus_tag="ACFM8U_000644"
     CDS             complement(7559..8734)
                     /gene="ctrA"
                     /locus_tag="ACFM8U_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HFC6333924.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8869..10002
                     /gene="neuC"
                     /locus_tag="ACFM8U_000645"
     CDS             8869..10002
                     /gene="neuC"
                     /locus_tag="ACFM8U_000645"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFC6333925.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGAAVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            10006..10692
                     /gene="neuA"
                     /locus_tag="ACFM8U_000646"
     CDS             10006..10692
                     /gene="neuA"
                     /locus_tag="ACFM8U_000646"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HFC6333926.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            10693..11742
                     /gene="siaC"
                     /locus_tag="ACFM8U_000647"
     CDS             10693..11742
                     /gene="siaC"
                     /locus_tag="ACFM8U_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HFC6333927.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            11826..13313
                     /locus_tag="ACFM8U_000648"
     CDS             11826..13313
                     /locus_tag="ACFM8U_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HFC6333928.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
     gene            complement(13809..14969)
                     /locus_tag="ACFM8U_000649"
     CDS             complement(13809..14969)
                     /locus_tag="ACFM8U_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6716 putative glycosyltransferase"
                     /protein_id="HFC6333929.1"
                     /translation="MKKVLLIASYDSFLNSGYAVAKEIKDAQIDIYIHKSRENILSNR
                     QLLESGIDKDQAIFFFHIDDYFIKNMHQYYDAVILSVGNGLLKRFFKQNAQLNIASRP
                     LIITLFPGVVFGDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIF
                     RHASKGRHGEKIYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDIT
                     VHQDKHSYIELAKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGV
                     VADLGFSKSYANQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLL
                     KQVVPLQHDYQEYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            15146..16165
                     /gene="galE"
                     /locus_tag="ACFM8U_000650"
     CDS             15146..16165
                     /gene="galE"
                     /locus_tag="ACFM8U_000650"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC6333930.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPFEIKPRRAGDLACFYADPSYTKAQIGWQTQRDLSQMMEDSWRWVSNHP
                     NGYDD"
     gene            16281..>16796
                     /locus_tag="ACFM8U_000651"
     CDS             16281..>16796
                     /locus_tag="ACFM8U_000651"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFC6333931.1"
                     /translation="MRKILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESL
                     TDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNI
                     VGTFNLLEAARAYWQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPY
                     SASKASSDHLVR"
BASE COUNT         4821 a         3904 c         3433 g         4638 t
ORIGIN      
        1 cggacgaggt ggtcgctgga cgctttagag gcagagtagg ggctggacgg cgcgtagggc
       61 gcggtttcgg taaacaaatc gtccgtgccg cctaaatcgc catagacttc atcggtggaa
      121 atatggtgga aacggaaagc ttcgcgcttt tcagacggca tttgctgccg gtaggcacgc
      181 gcagcttcaa gtaggttgaa tgtgccgacg atattggttt ggataaactc gcctgccgaa
      241 ccgatagagc ggtcgacatg gctttccgcc gccaagtgca tcacggcatc tggccggtgt
      301 tgcgcgaata cgcggtcgag ttcggcgcgg tcgcaaatat ccacttgttc aaaagcatag
      361 cgaggattat cggctacctc agtcaaagat tccaaattgc cggcataagt cagcttatcg
      421 acattgacga cagcgtcccg ggtgtttcgg ataatatgac ggacaacggc agaaccgata
      481 aagcccgcgc cgccggtaac gaggatggtt tttttgtttg cggtttgcat ttaatcccca
      541 tatccgttgg ggttgcggct gacccaacgc cacgaatctt ccatcatttg ctgcaagccg
      601 cgtttggttt cccagccggt ttgttgtttg gtatgggacg ggtcggcaaa ggaacacgcc
      661 aagtctccgg cgcgtcgggg ttggatttga tagggaatat ttaagccgga tgcggcttca
      721 aaggcacgga tgatttccaa aacggaatag gcgcgccccg aacccaagtt gaacaaatgt
      781 acgccggcaa cgccgccttt tgccttcatt gccgcgatat gcccttctgc caaatccatc
      841 acatgaatgt agtcgcgcat tcccgtaccg tcgggggtcg gataatcgcc gccgaatacc
      901 gacagttgcg gcagcctgcc cgaagccact tggcagatat agggcaaaag gttgttggga
      961 ataccgttgg gctgttcgcc gatcaagccg ctttcgtgcg ccccaatcgg gttgaaatag
     1021 cgcaacaaaa tcacgctcca acgcggatcg gccttttgga tgtcggttaa catccgttcc
     1081 accatcgctt tggatgcgcc ataaggatta gcggtatcgc ccgggcgcat atcttccgta
     1141 tagggcattt tacgtatcat ggcgcgacgc attaaagtaa acgccaaagc gcgtttatac
     1201 tggttatcca gtttccctat atttttacgg tacaaatcaa gttgttggca ttcatcgtga
     1261 aaatagaatt tttcggcata acgctgactc ataaaaccgg catgcgggct gcctgaaaaa
     1321 atcattgcga catcatcgtc atttaatgtt tcatggttgt tttctattaa tgctaaagca
     1381 atttggtaat taattgccaa aatatcgtta gtaatcacac gatagccgcg ttttttggct
     1441 tcataggcaa aggaacagcc tccactaaat acatctgcaa ctgtatctac atcagacgga
     1501 agctggtcac aaatccagga agctattttc tctttattac cgatatagtt aattttcgga
     1561 tattgcttat tcattttcac tcatttttaa aatactttca gcaatagctt ttgccaataa
     1621 aggcggtacg gcgttaccaa cctgctgctg ctgggcaatt ttgctgccgc aaaaaataaa
     1681 attatcaggg aaagattgta aggcagctaa ttcacgaacg gttaacgccc gattctgttc
     1741 atagtgaaaa actttgcgca tatctcctgt aatacaaacg gctggttttg ttgctgttgt
     1801 aacggatgta tttacggata tcacctgttt tcggacgtaa tggttcagga atatcgttac
     1861 ggttacctcc atttttaaca aatgccattt tttctaacat ttgtgccgaa tgattcatag
     1921 cttcatgatt tgcaacgtgt ggattgcttt cgccagcagc cagttttgga aaatgtccta
     1981 ttgctgatcc aacagtctga tgggaaatct gcaaaggttc gggaaaggaa attttgcctt
     2041 tatccctcct cccgataaat atcactcggc tacgtatctg aggaacaccg aaatcggctg
     2101 cactcagtat cttacattcc accgaataac cgatattctg aaatgcttga ataatctcaa
     2161 tacgtgtttt acctgaattg tgtgtataga gtcgcgctac attttccata acaaaaaaat
     2221 atggttggac aattttaact attcggacaa actctttaaa taaatggttg cgtgggtcat
     2281 ctgtaaatgt ccgtccaatc tttcctgcca tactaaaacc ttgacaaggt ggtcctccaa
     2341 taatcaaatc aactgcttgt ccgttaagac aattgattaa atcttgttcg gttagtgtgg
     2401 ttaaatcttt ttgcagtaat tgatgatggg ggaagttggt acggtaagtc tgacaataat
     2461 cagactccat ttcaacagaa agcaattgtt ggaatccggc ttgttcaaaa cccaaggata
     2521 ggcctcctgc tcctgagaaa aggtcaatat aggtaagtgg tgtgtgcata agtcaaaatc
     2581 cataaactct tcgattattt taccgcttca gcttcgcaaa cgcatccgcc atcgccgaat
     2641 tggctggggc gcggtcgttg cgttggggtt tgcggccggc tgtccgttcg cggctgcggt
     2701 tttcagacgg cattttatgt tttgcgccgc ccggttcgtc atccaagcgc atggtcagcg
     2761 cgatgcgttt gcgtgcagca tcgacttcca gcactttcac tttcaccacg tcgccagctt
     2821 tcaccacttc gcgcgggtct tggacgaact tgttggacag ggcggagatg tgcaccaagc
     2881 cgtcctgatg gacgccgatg tccacgaacg cgccgaagtt ggcgacgttg gaaaccacgc
     2941 cttcgagtat cataccgact tgcaagtcgc tgatttcgtg gataccttcg gcaaacgatg
     3001 ccgtctgaaa ctcgccgcgc ggatcacggc caggtttttc cagttcggac aggatgtcca
     3061 aaatggtcgg caggccgaag cgttcgtcgg tgaagtcgga cgctttgatt tgcttcacgc
     3121 gctcgcggtt gccgatgagt tcggcggcgc taatgccttg ttgcgccagc attttggcga
     3181 cgacgggata ggcttcgggg tggacggcgc tcgcgtccaa cggctcttta ccgccgttaa
     3241 tccgcaaaaa gcctgccgcc tgctcgaagg ttttttcgcc caaacgcggt actttcagca
     3301 attttttgcg gctgtcgaac gcgccgtttt catcgcggta ggcaacgatg ttttgggcaa
     3361 gggtttgatt caaaccggaa attcgcgcca agagcggggc ggaggcggtg ttcacgtcca
     3421 cgccgacggc gttcacgcag tcttcgacca ccgcgtccag cgatttggcg agctggcttt
     3481 ggttcacgtc gtgctgatac tgccccacgc cgatggattt ggggtcgatt ttgaccaact
     3541 cggcaagcgg gtcttgtagc ctgcgggcga tggacaccgc gccgcgcagg gaaacgtcca
     3601 agtcggggaa ctcgcgcgcc gccagttcgg acgcggaata aatcgacgcg ccggcttcgg
     3661 acacgacgat tttatgcagc cccattttcg acatttcacg caccagttcg cccgcgattt
     3721 tgtcggtttc acggctggcg gtgccgttac cgatggcgat gagcttcacg ccgtgctgtt
     3781 taatcaggcg cgacagcgtt gccaacatat tgttttcttg atgcaaatag acgatgacgg
     3841 tatccagcag cttgccggtg tcgtccacca cggcgcattt cacgccgttg cggtagccgg
     3901 gatcgagacc cagcgtggtc agccgtccgg cgggcgcagc aagcagcaag tctttgagat
     3961 tgcgggcgaa cacggtaatc gcgtcggtgt cggcggcttc tttcaaacga ttgagagctt
     4021 caagttccaa cgacaaaaag attttcgcgc gccaagttag gcgcacggtg tcgcgcagcc
     4081 atttgtggcc gtctgaaact ttgaagcggc gggcgatgat ttgctcgtat tcgctttgct
     4141 gcgtaatcgg cgtgtcgtcg ggctgatatt tgagcgcgat gttcaacacg ccttcgttgc
     4201 gtccgcgcaa aaccgctagc gcacgatggc tgggcatcgc acgaataggt tcgcggtggt
     4261 cgaaataatc gctgaatttt tcgccttcgg tttctttgcc tccaacaact tgcgcatgga
     4321 tttcggcttc gttccacaac ttatcacgca gcgtgccgat gagttccgcg tcttcggcaa
     4381 actgctccat cagaatcgca cgcgcaccgt ccaacgcggc tttggcatcg gggacgtttt
     4441 cgttcaggta gccttgcgcg gcagcttcca cgtccagcgg ctgctcggca agcaacacgt
     4501 ccgccagcgg ctgcaaaccg tgttcgcgcg cgatttgcgc tttggtgcgg cgtttgggtt
     4561 tgtagggcag atacaggtct tccagcgcgg ttttgttatc ggcggcttcg atttgcgccc
     4621 tgaggtcgtc tgaaagcttg ccttgctctt caatgctttt taaaacaacg gctttgcgct
     4681 cttccaactc gcgcagatat tgcagccgct cggcaagccg gcgcaactgc gtatcgtcca
     4741 acccgcccgt cgcttccttg cggtagcggg cgataaacgg cacggtcgcg ccgtcgtcca
     4801 aaagctcgac ggcggcggtg atttgcgcgg cagtcgcgga gagttcttgg gaaagaattt
     4861 gagtaatgtt catcaataga attccaacgg acaggccgtc tgaaatttca gacggcctga
     4921 tttaaaaaca atcgctttaa ggcagcgaat tataatattc gtaggctttg tccatatctt
     4981 caaactggta catatgccct ttttccagca ccatcgcatt atcgcaatat tgcttcatgg
     5041 cgctgtggct gtgcgacacc aagatgatgg aacggtcttt gcgcttttca aacaactcgt
     5101 acttacattt atcggcaaaa cgcgagtcac caactgcaat cacttcgtca atcaaatagc
     5161 aatcaaactc taccgccaac gacaaagcaa atgccaaacg cgccttcata cctgaagaat
     5221 aacgcttgac cggctcgtaa agatattgcc ccaactcgga aaactcttcg gtaaagttct
     5281 tcacataatc gatatcgaca ttgtaaatcc ggcagatgaa acgcaaattg tccataccgg
     5341 tcagactgcc ttgaaacgca ccggagaatg ccaaaggcca agaaatactc attgtccgct
     5401 tgatttcacc cgtggtcggc ggctcaacgc cactgatcaa acggatgagc gtcgatttac
     5461 ctgcaccgtt gcggccgaga ataccgattt tctcgccctt ctccattttg aagctaatat
     5521 cgtgcaagac tgtccgccaa ccttggcggg tcagatagcg tttggaaacg tgttcaactg
     5581 aaatcattgc ggctcaaccc ctttgctgaa tctcgccacc accgccaaac caagcagcag
     5641 caaaaccaag ttacacaaca aaatatacca aggattttcg taagtcgtga cgctgtcgcc
     5701 aaaatatccg gcacggaaca tttctgtgcc atgcaccatc gggatcatca atgcatactc
     5761 ttgcactttc ggaggcaaat tatgtacaaa aaagaatgca cccgataacg gcatcatcac
     5821 aaaagtcaat gtgccccaaa tcttgccaaa cggctcgaaa ttaaaggcaa tcgaacaaat
     5881 caccaaaccc aaaccaatcg caaaaaaagc catcaaaagc caagccatca gcatataaaa
     5941 catatctgcc ggcatttcaa tccagccaat cgcaatcaat accgccataa tcacaatctg
     6001 cgcaatggtt gcaccagcaa tttccaaaat catgcgcgcc aagatggtat ccaaaactct
     6061 tacattgcgg tgataaagca agctggcatt tgaagaaatc gacccaactg cccgttttga
     6121 ggcattacgc cacatcatca acatcggata gccagtaatc gcaaatgcga caatattcaa
     6181 agttgaatat cggtctgcct ttaaaaattt ccacatcaag acgataacga atgtcatcag
     6241 caacggctca acaaacagcc ataaaaagcc aatattattg cgaccgtaac gggtgataat
     6301 ttcccgcatc aaaagcgcac cgattacgcg cctttgaatg gctaaagatt cccaaaatga
     6361 tgttttatgc aaggctttca tcagttttta tgctcacgaa tgctggcagt caacaggctc
     6421 aaaataccat aaaccatcaa gccgataatc agagtggcaa caatgttgta taaccgttta
     6481 ggctcatgtg ccaaatccgg caggctcggt tgcgagatca cttccaaata aagctgctga
     6541 cggtctgctt caaccttggc actttccaaa gaagtcatgg cggctgccaa ctgctgctct
     6601 gccaactggt tttccaaata cacacgctga tattcggcag cctgattaga caacgaagaa
     6661 tgcccaccgc ccgaaatggc acgtaactgt tggtcaattt ctttacgcaa gctctgctca
     6721 cgcgcctgca aacccggaat ctgcggattc tccggagtga ctgctttcac ctgatccagc
     6781 tgggtttgaa tcacaatcaa ttcatcttgc agcttggaaa ccaaccccat ttgcacttcc
     6841 gattgcgctt tcaaatcaaa aacgccattg gcaatccggt aatccgtcag attctgagag
     6901 gcttccttta cccgctctgc cgccgttttc actacttctt ccgcatagcg caccgtatca
     6961 gcacgtgcac gatcgttcaa ctggttaatc aatgcttcac cttgttttaa caaagcctca
     7021 ttgattttct tagattccag cgcatcaaag gaagttacat tcaacgtgga aatacccgaa
     7081 accgtatcaa aattgatcat cacctgattt ttatagtatt gataaaaagc ctcttcctcg
     7141 ccacggaacc caaacccatt aaagcggctg aacgcatcac ctttggtttc ataaaactca
     7201 cgcaccggca agattttacg cagttcatcc aaagacgaac gcgactgcat atattcctgc
     7261 acggtataaa tatcgtcttg cgcacgggca aaacctgtgc cctgcaaaat ggcacccagg
     7321 ccattgagag aagattggct tttaggcgag cgcaccacaa agctcgattg cgacgtaaaa
     7381 cgatcggaag cgaagaagcc gaaatacacc aacgaaatta ccgtagggat aatcaccgtt
     7441 acccaaaata aagggcttag ctttttaatc caacttttct ttttcggctt tttacgctcg
     7501 gctttggttt cagtggcaac tgccacagga agttgctcag acatcttaat tactcacatt
     7561 aattagttaa attattaata ctgttcgcgc cactggtaac cggcgagaac acaaacgaca
     7621 aaaatttctg cacttcagcc aacggcgcat tcgacacata caatacatct ttattcttca
     7681 caggaaagcg ctgcatagaa aatagcgaat gcgcatcagc catattcaca cgatataccg
     7741 ttggaatctc tgcctcactg ccataacctt gagcaatcca tttatcctga cgttctgccg
     7801 gcaattccac caatggcgta tagcggaaca caaacacacc acgcgcatca gaacggcgat
     7861 cttgcaaacc gcccatacgg ccaatggctt cagaaagcga taagcctctg gctgaaaaac
     7921 cgatttcttg tgttctcccc accgcaccca tagacgtaaa ggtataggga ttggtaatca
     7981 tggtaaccac atcaccgcga cgcagcaaaa tattttgtcg cggatttgca actaaatctt
     8041 ccaaggcaac agttcgtact acattgccac gtgtcagctg cacattcgta tcctgcacat
     8101 ttgccgttga accacctacc gcagccaccg catccaacac acgctcaccg gctgccgtca
     8161 gcggcatacg cacactattc ccagcacgaa tcaccgacac attcgccgca ttattctgca
     8221 ccaaacgcac catcacttgt ggctgattgg ccattttttt caggcggcct ttaataattt
     8281 cctgaacctg accaggcgtt ttaccgacca ccgaaatatc gccaacaaac ggcacagaaa
     8341 ccgtaccacg tgccgtgacc aactgctctg gcaacttagt ttgatgcgca ctacccgagc
     8401 ccatcgaaga aaggccacca ccaaacaata ctgccggcgg cgcttcccaa atcataatat
     8461 ccaatacatc accaatattt agcgtaccag ccgaagcata accatcgcca aactgagtga
     8521 atgactgatt tatctgagcc ttatataata actgagcaac cgtatgattc acatcaatca
     8581 gctccacttc aggaatttga acttcagatt gttgccctaa agagacaatt ttttttgcgc
     8641 tggggcctga tgaaggaatc gcagagcatc ctacaattaa acttccacac aataataata
     8701 ctgcgtgacg aatataaaat ttcactttaa acacaagcca aatcctaata taattataaa
     8761 tggcctaatt atagcactta atcgaaataa atttatgagt acgtagagta taattagtat
     8821 tcttctttcc aacttcctta tacttatata cttatagatt ctaaaatcat gaaaagaatt
     8881 ctttgcatta caggtaccag agccgacttc ggcaagctaa aacctttatt agcctatatt
     8941 gaaaatcacc cagaccttga attgcatttg attgtaactg gtatgcatat gatgaaaaca
     9001 tatggcagaa cctacaagga agtaactcga gaaaactatc aacatacata tctgttttca
     9061 aatcaaatcc aaggtgaacc aatgggtgcc gttttaggca ataccattac gtttatctct
     9121 cgtctatctg atgaaattga acctgatatg gtcatgattc acggcgaccg tttagaagca
     9181 ctagcaggcg cagctgtagg tgcattaagc agccgtttag tttgccatat cgaaggtggt
     9241 gaactatctg gtacagtaga tgactccatt cgtcattcta ttagtaaact ttctcatatc
     9301 cacttggtag caaatgaaca agctgtcact cgcctagtgc aaatgggaga aaaaagaaag
     9361 catattcaca tcatcggctc ccccgattta gatgttatgg cctcttccac cctcccatcc
     9421 ttagaagaag tcaaagaata ttacggttta ccatacgaaa attatggtat ttctatgttt
     9481 caccccgtga ctacagaagc acatttaatg ccacaatatg cggcccaata tttcaaagca
     9541 ttagaattaa gtggccaaaa tatcattagc atctacccta ataatgacac tggcactgaa
     9601 agtattctgc aagaattatt aaaatatcaa tctgataaat ttattgcatt tccttccatt
     9661 cgatttgaat attttttagt cttattgaaa catgctaaat ttatggtcgg caactcaagt
     9721 gcaggtatta gagaagctcc tctctacggt gtcccttcaa ttgatgttgg tacacgccaa
     9781 agtaaccgcc atatgggaaa atctattatt catacagatt atgaaactaa aaatatcttt
     9841 gatgcgattc aacaagcatg cagtttaggc aaatttgaag cagatgatac ctttaatggc
     9901 ggagatactc gcaccagcac agaaagattt gctgaagtaa tcaacaatcc tgaaacgtgg
     9961 aatgtttctg ctcaaaaacg ttttatcgat ttgaatcttt aaattatgga aaaacaaaat
    10021 attgcggtta tacttgcgcg ccaaaactcc aaaggattgc cattaaaaaa tctccggaaa
    10081 atgaatggca tatcattact tggtcataca attaatgctg ctatatcatc aaagtgtttt
    10141 gaccgcataa ttgtttcgac tgatggcggg ttaattgcag aagaagctaa aaatttcggt
    10201 gtcgaagtcg tcctacgccc tgcagagctg gcctccgata cagccagctc tatttcaggt
    10261 gtaatacatg ctttagaaac aattggcagt aattccggca cagtaaccct attacaacca
    10321 accagtccat tacgcacagg ggctcatatt cgtgaagctt tttctctatt tgatgagaaa
    10381 ataaaaggat ccgttgtctc tgcatgccca atggagcatc atccactaaa aaccctgctt
    10441 caaatcaata atggcgaata tgcccccatg cgccatctaa gcgatttgga gcagcctcgc
    10501 caacaattac ctcaggcatt taggcctaat ggtgcaattt acattaatga tactgcttca
    10561 ctaattgcaa ataattgttt ttttatcgcc ccaaccaaac tttatattat gtctcatcaa
    10621 gactctatcg atattgatac tgagcttgat ttacaacagg cagaaaacat tcttaatcac
    10681 aaggaaagct aaatgcaaaa caacaacgaa tttaaaattg gtaatcgttc agtaggttac
    10741 aaccacgaac cattgattat ctgtgaaatc ggcatcaatc atgaaggctc tttaaaaaca
    10801 gcttttgaaa tggttgatgc tgcctataat gcaggcgctg aagttgttaa acatcaaaca
    10861 cacatcgttg aagacgaaat gtctgatgag gccaaacaag tcattccagg caatgcagat
    10921 gtctctattt atgaaattat ggaacgttgc gccctgaatg aagaagatga gattaaatta
    10981 aaagaatacg tagagagtaa gggtatgatt tttatcagta ctcctttctc tcgtgcagct
    11041 gctttacgat tacaacgtat ggatattcca gcatataaaa tcggctctgg cgaatgtaat
    11101 aactacccat taattaaact ggtggcctct tttggtaagc ctattattct ctctaccggc
    11161 atgaattcta ttgaaagcat caaaaagtcg gtagaaatta ttcgagaagc aggggtacct
    11221 tatgctttgc ttcactgtac caacatctac ccaacccctt acgaagatgt tcgattgggt
    11281 ggtatgaacg atttatctga agcctttcca gacgcaatca ttggcctgtc tgaccatacc
    11341 ttagataact atgcttgctt aggagcagta gctttaggcg gttcgatttt agagcgtcac
    11401 tttactgacc gcatggatcg cccaggtccg gatattgtat gctctatgaa tccggatact
    11461 tttaaagagc tcaagcaagg cgctcatgct ttaaaattgg cacgcggcgg caaaaaagac
    11521 acgattatcg cgggagaaaa gccaactaaa gatttcgcct ttgcatctgt cgtagcagat
    11581 aaagacatta aaaaaggaga actgttgtcc ggagataacc tatgggttaa acgcccaggc
    11641 aatggagact tcagcgtcaa cgaatatgaa acattatttg gtaaggtcgc tgcttgcaat
    11701 attcgcaaag gtgctcaaat caaaaaaact gatattgaat aatgcttatt aacttagtta
    11761 ctttattaac agaggattgg ctattacata tagctaattc tcattaattt ttaagagata
    11821 caataatgct aaagaaaata aaaaaagctc tttttcagcc taaaaagttt tttcaagatt
    11881 caatgtggtt gacaacatct ccattttatc ttaccccccc acgtaacaat ttatttgtca
    11941 tatctaattt aggtcagctt aaccaagtcc aaagcctaat taaaatacaa aaattaacca
    12001 ataatttact agtaatttta tatacttcta aaaacttaaa aatgcctaag ttagttcatc
    12061 aatcagctaa caagaatcta tttgaatcta tttatctatt tgagcttcct agaagcccta
    12121 ataatataac tcctaaaaaa ttactttata tttatagaag ttacaaaaaa atccttaata
    12181 ttatacagcc tgctcatctc tatatgctgt cttttacagg ccactactcc tatctgatta
    12241 gtattgcaaa aaaaaagaat attacgactc atttaattga tgaagggact ggaacatatg
    12301 ctcctttatt agaatcattt tcatatcatc caacaaaatt agaacgttat ttgattggaa
    12361 ataatcttaa tattaaagga tatatagatc attttgacat attgcatgtc ccctttcctg
    12421 aatatgctaa aaaaatattt aatgcaaaaa aatataaccg gttttttgcg catgctggag
    12481 gaataagcat taataataac attgcaaact tacagaaaaa atatcaaata tctaaaaatg
    12541 actatatttt tgttagtcaa cgctacccca tttcagatga tttgtattat aagagtatag
    12601 tagaaatctt aaacagcata agtttacaaa ttaaaggaaa gatatttatt aaactacacc
    12661 caaaagagat gggtaacaac tatgtaatgt ctttatttct aaatatggta gaaataaacc
    12721 ctcggctggt agttattaat gaacctcctt ttctaattga gcccctaata tacttaacaa
    12781 atcctaaagg aattataggc ctggcctcta gttctttaat ttatacacca ttactctcac
    12841 cctcaaccca atgtctttct attggagagt taattattaa cttaattcaa aaatattcaa
    12901 tggtggaaaa cactgaaatg atccaagaac acttagagat tattaagaaa tttaatttta
    12961 ttaatatact aaatgattta aatggggtaa taagtaaccc tctctttaaa acagaagaaa
    13021 catttgaaac acttcttaaa tctgcagaat tcgcatataa atctaaaaac tactttcagg
    13081 ctatttttta ctggcaactt gccagcaaaa acaatattac cttattaggg cataaagcat
    13141 tatggtacta caatgcactt tataatgtaa aacaaattta taagatggaa tattcagata
    13201 ttttttatat cgataatatc tccgtagact ttcatagtaa agataaattg acatgggaaa
    13261 aaattaaaca ttattactat tccgccgaca atagaattgg tagagataga taaaaatact
    13321 atgacttctg atctccatac gtatgcttgt tttcaaaaag cagcattaag atagataagt
    13381 gaagcttgat gagagccatt tttgtggtca tcgccaaggt acacgcggag agtaatcgac
    13441 aacagagctg ctgtattctg gcttttaaag cccagtagta aagtttataa ggctgcattt
    13501 ctcaatgtaa aacgcgatat tttcacccat tattagggat aagtagccag tggtttacac
    13561 tggctacttt catagttatg aagtactggg tctgagtgaa ttcaattatt tccgtataaa
    13621 acacagtaga cattttacta atcggcaaaa ttatattaga agaattgaga actactgaag
    13681 tcaggtaaga taccattttg cacaaattta acggcatccc taaaaagcat tttgggtggt
    13741 atttaaagga gtacaaaatg gttttaacta cagtatgctt agaaatctat agccttacgc
    13801 gctttatatt aaatcacctt tctcaaccat ttagaatcat aaaaaaactt tttaggatcc
    13861 ctaattaact ttttaaattt tcttaaaaaa atagttttag ttgattctat actattcacc
    13921 gcagataaat attcttggta atcatgttgc aatggcacaa cctgctttaa tagcttatta
    13981 aactcatcag ttgtaatcac ctttttagta gcatagcaat caagccaatc cggatctgct
    14041 atttttgggg aagtgaaatc tatttgatca aaataaacta aaaccccact acctaaaaaa
    14101 tgctgatttg cataggattt agaaaagcct aagtctgcaa caacaccaac agggataccc
    14161 ttacattcag cttcaaaaag catagtagat gaataagata aacaataccc catttcttgg
    14221 aaggcttgcg cggtactttt tcgctctatt gtcaaattac tcggcaactg aaactgcttt
    14281 gccagctcta tatacgaatg tttatcctga tgcacagtaa tatctttatc tgcaaccctt
    14341 agcaaaatag taaattcttt ctcagggtat ttttcagcca aggcaatcaa tttttttaat
    14401 gtataaattc tttcttcttt tttaaatggg attttaactt ggtcaataaa gtaaattttc
    14461 tctccatgac gaccttttga tgcgtggcga aaaattggat aaccataaag tatattttga
    14521 caacttaatt tatattgttt cttatattcc tctgcaattc taaaatcatg cttattatta
    14581 tataaaacaa tatcagcccc catacgagat agaatacttg cctgatcacc gaatactaca
    14641 cctggaaaca aggtaataat caatggcctt gaagcaatat ttaattgcgc attctgctta
    14701 aagaaccttt ttaacaaccc atttccaacc gataaaatta ctgcgtcata atattgatgc
    14761 atattcttaa taaagtaatc atcaatatga aaaaaaaaaa ttgcttggtc tttatctatc
    14821 cctgattcta ataactgtcg atttgaaaga atgttttctc gacttttgtg gatataaata
    14881 tcaatttgag catcttttat ctcttttgca acagcatagc ccgagttaag gaacgagtca
    14941 taactggcaa ttagtaatac ttttttcact attttccctt aattctacac catatgaata
    15001 aaattatagt attttacctt agatatcaaa tatacttaac caaaaaaatc tgaagaaatc
    15061 tactgctact tcagggataa ttatttcatt aagattttac aaccattatt atcaatctat
    15121 caaagtattt ttagtatttt attctatgaa aaaaattctc gttaccggcg gcaccggttt
    15181 tatcggctcg cacaccgttg tttctttgct gaaaagcggt catcaagtcg tgattttgga
    15241 taacctatgc aattccagca tcaatatcct gccacgcttg aaaacgatta ccggccaaga
    15301 gattccgttt tatcaaggcg atatccgcga tcgtgagatt ttgcgccgta tttttgcgga
    15361 aaaccgcatt gattcggtga ttcattttgc cggcttgaaa gcggtgggtg aaagtgtggc
    15421 cgagccgatg aaatattatg ataataatgt ttccggcagc ttggtgttgg cggaagaaat
    15481 ggcgcgtgcg ggcgtgttta gcattgtgtt cagttcttcg gcgacggttt atggcgatcc
    15541 gggcaaagtg ccttataccg aggatatgcc actgggcgat accaccagcc cttacggcac
    15601 atcgaaatca atggttgagc gcattctcac tgacattcaa aaagccgatc cgcgctggag
    15661 catgattttg ttgcgttatt tcaatccaat tggcgcgcat gaaagcggct tgattggcga
    15721 gcagccaaac ggtatcccaa ataatttgtt gccttatatc tgccaagtgg cggcaggcaa
    15781 actgccgcaa ttagcggtat tcggcgatga ctaccctacc cctgatggca cgggtatgcg
    15841 cgactatatt catgtgatgg acttggctga aggccatgtc gccgccatgc aggcgaaaag
    15901 caatgtagca ggcacgcatt tgttgaactt aggttccggt cgcgcttctt cggtattgga
    15961 aatcatccgc gcatttgaag cggcttcggg tttaaccatt ccatttgaaa tcaaaccacg
    16021 ccgtgcgggc gatttggcgt gtttctatgc tgacccttct tatacgaagg cacaaatcgg
    16081 ttggcaaact cagcgtgatt taagccaaat gatggaagat tcatggcgct gggtaagtaa
    16141 tcatccgaat ggctacgacg attaagttga cctgatacag gccgtctgaa agagatgttt
    16201 tcagacggcc tctttatctg aaaaacacac attctgtctg ctataatctg tttatatttt
    16261 ttggctatcc tctgaaattt atgagaaaaa tccttgttac cggcggtgcg ggctttatcg
    16321 gttctgccgt tgtccgtcat attatccgaa acacccaaga ttccgtcgtc aacctcgaca
    16381 aactgaccta cgcgggcaat ctcgaatcgc tgaccgacat tgccgataat ccccgctatg
    16441 ctttcgagca agtggacatt tgcgaccgcg ccgaactcga ccgcgtattc gcacaacacc
    16501 ggcccgatgc cgtgatgcac ttggcggcgg aaagccatgt cgaccgctct atcggttcgg
    16561 caggcgagtt tatccaaacc aatatcgtcg gcacattcaa tctgcttgaa gcagcccgcg
    16621 cctactggca acaaatgccg tctgaacagc acgaagcctt ccgtttccac catatttcca
    16681 ccgatgaagt ctatggcgat ttaggcggca cggacgattt gtttaccgaa accgcgccct
    16741 acgcgccgtc cagcccctac tctgcctcta aagcgtccag cgaccacctc gtccgc
//
LOCUS       DAXHSJ010000026        16675 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000026 DAXHSJ010000000
VERSION     DAXHSJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.026"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(76..1245)
                     /gene="metK"
                     /locus_tag="ACFM8U_000652"
     CDS             complement(76..1245)
                     /gene="metK"
                     /locus_tag="ACFM8U_000652"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC6333932.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEKLKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1428..1538)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1591..2487
                     /locus_tag="ACFM8U_000653"
     CDS             1591..2487
                     /locus_tag="ACFM8U_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6333933.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     TEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2531..3595)
                     /gene="tsaD"
                     /locus_tag="ACFM8U_000654"
     CDS             complement(2531..3595)
                     /gene="tsaD"
                     /locus_tag="ACFM8U_000654"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC6333934.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRFEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3711..4898)
                     /gene="ccsB"
                     /locus_tag="ACFM8U_000655"
     CDS             complement(3711..4898)
                     /gene="ccsB"
                     /locus_tag="ACFM8U_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC6333935.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4891..6843)
                     /locus_tag="ACFM8U_000656"
     CDS             complement(4891..6843)
                     /locus_tag="ACFM8U_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC6333936.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTVSREGGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7120..7743)
                     /locus_tag="ACFM8U_000657"
     CDS             complement(7120..7743)
                     /locus_tag="ACFM8U_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6333937.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7949..8578
                     /gene="yihA"
                     /locus_tag="ACFM8U_000658"
     CDS             7949..8578
                     /gene="yihA"
                     /locus_tag="ACFM8U_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC6333938.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8699..11095)
                     /locus_tag="ACFM8U_000659"
     CDS             complement(8699..11095)
                     /locus_tag="ACFM8U_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC6333939.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11247..12362
                     /gene="pilM"
                     /locus_tag="ACFM8U_000660"
     CDS             11247..12362
                     /gene="pilM"
                     /locus_tag="ACFM8U_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC6333940.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12365..12964
                     /locus_tag="ACFM8U_000661"
     CDS             12365..12964
                     /locus_tag="ACFM8U_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC6333941.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12965..13612
                     /locus_tag="ACFM8U_000662"
     CDS             12965..13612
                     /locus_tag="ACFM8U_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC6333942.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13630..14175
                     /locus_tag="ACFM8U_000663"
     CDS             13630..14175
                     /locus_tag="ACFM8U_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC6333943.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14194..16503
                     /gene="pilQ"
                     /locus_tag="ACFM8U_000664"
     CDS             14194..16503
                     /gene="pilQ"
                     /locus_tag="ACFM8U_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC6333944.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQQAAAPAKQQ
                     AAAPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRS
                     LDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESG
                     GVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWD
                     QALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKY
                     KNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDE
                     LDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKW
                     GAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAK
                     IESGYEIPFTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQC
                     ASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFK
                     TRGKKTDRRELLIFITPRIMGTAGNSLRY"
BASE COUNT         4509 a         4744 c         3802 g         3620 t
ORIGIN      
        1 gtcctgattt ttgttaatcc actatataaa aatgccgtct gaaccttcag acggcatttc
       61 tttattcaga ccaaattaca gaccggcggc tgccctcaat gcggctgctt tgtcggtgcg
      121 ctcccaagta aactcgggtt cttcgcggcc gaaatgtccg taagcggcgg atttactgta
      181 aatcgggcgc aagaggtcga gcatttggac gatgcctttg gggcgcaggt cgaaatgttc
      241 gcgaactaag gtaatcagtt tttcttcgct gattttgcct gtgccgaaag tatcgatgga
      301 aatcgaagtc ggttcggcaa cgccgatggc gtaggaaact tgaatttggc attgggttgc
      361 caagcctgcg gcgacaatgt ttttcgccac ataacggcag gcataggcgg cggaacggtc
      421 gactttggac gggtctttgc cggagaacgc gccgccgccg tgcggagccg cgccgccgta
      481 ggtatcgacg atgattttac ggccggtcaa accgcagtcg ccttgcggac cgccgatgac
      541 gaagcggccg gtcggattga tcaggtattt ggtttcgtcg gtcagcagtt cagacggcag
      601 gacgggcttg ataatctgct cgatgacggc gtttttcagt ttttcgtaag cgatggacgg
      661 atcgtgctgg gtagagagga cgacggtgtc gatgcgtttt actttgccgg tttcgctgtc
      721 gtaaaccacg gtcagttggg cttttgcgtc agggcgcagc caaggcaggc ggccgtcttt
      781 gcgtaattcg ctttgacgct gcatcaggcg gtggctgtaa tagatggcaa acggcatcag
      841 ggtcggggtt tcgtcgcagg catagccgaa catcaagcct tggtcgcctg cgccttggtt
      901 caagtcgatg ccttcgcctt cgtttacgcc ttgggcaatg tccggagatt gttggtcgta
      961 gtacacgccg accgcgcagc cgttggcatc aaaacccaac tcggaggagt tgtagccgat
     1021 acgtttgatg gtttcgcgtg cgactttgat gtagtccact tgggcggtgg tggtgatttc
     1081 gcccgccaat acgcacaagc ctgtgttgac caaggtttct gcggcgacac gtgctttagg
     1141 gtcttgcgcc aaaatggcat ccaaaatcgc atcggacact tggtcggcta ctttgtccgg
     1201 atggccttcg gataccgatt cggaagtaaa cagatattcg ctcattgaga ttttccttga
     1261 aaaacaaacc atcttcgtta gacggcatgt tgtatgaaca taatgtcgac agcgggaaat
     1321 atagcaaaat ttccctattc ataccattca gttgagaaat attcccattt gaacagcact
     1381 ttggaatctc tacccgtacg tttcttacag gcaaaaaaat tcccgcatta agcgggtttg
     1441 gattgctctg gtgagccaca tcggcttttc aaccgtccac cttacttttc cctttgaaaa
     1501 gcaggttggc atggaattcc caactcttaa tgcagcgcgg atggtagcag aaaagccttt
     1561 ccgccgcaat acttcccttt ttcagacggc atgtttcgtt tacaattcgg gctgtttccc
     1621 cctttgcgaa ccgccatgca catcctgttg accgccctgc tcaaatgcct ctccctgctg
     1681 ccgctttcct gtctgcacac gctgggaaac cggctcggac atctggcgtt ttacctttta
     1741 aaggaagacc gcgcgcgcat cgtcgccaat atgcgtcagg caggcatgaa tcccgacccc
     1801 aaaacggtca aagccgtttt tgcggaaacg gcaaaaggcg gtttggaact tgcccccgcg
     1861 tttttcagaa aaccggaaga catagaaaca atgttcaaag cggtacacgg ctgggagcac
     1921 gtgcagcagg ctttggacaa acacgaaggg ctgctgttca tcacgccgca catcggcagc
     1981 tacgatttgg gcggacgcta catcagccaa cggctgccat tccccttaac cgccatgtat
     2041 aaaccgccga aaatcaaagc gatagacaaa atcatgcagg cgggcagggt tcgcggcaaa
     2101 ggaaaaaccg cgcctaccag catacaaggg gtcaaacaaa tcatcaaagc cctgcgttcg
     2161 ggcgaagcaa ccatcgtcct gcccgaccac gtcccctccc ctcaagaagg cggggaaggc
     2221 gtatgggtgg atttcttcgg caaacctgcc tataccatga cgctggcggc aaaattggca
     2281 cacgtcaaag gcgtgaaaac cctgtttttc tgctgcgaac gcctgcctgg cggacaaggt
     2341 ttcgatttgc acatccgccc cgtccaaggg gaattgaacg gcgacaaagc ccatgatgcc
     2401 gccgtgttca accgcaatac cgaatattgg atacgccgct ttccgacgca gtatctgttt
     2461 atgtacaacc gctacaaaat gccgtaacga aaataaaaat gccgtctgaa caatttcaga
     2521 cggcattttg tcatctgacg atttccgaca gcggccagcg cggacggaca ttgaacgcac
     2581 cgacctccct gcccttgccc aggtgcatcg caccggcaaa ggcaatcatg gcaccgttgt
     2641 ccgtgcagta tgccatcggc gggaaaaaca cgctgacttt ttcggacgga tgtttcggct
     2701 tgcctttggg ggtcgggatt tgcaccgtca tgttgccgaa agtttcgcgg agtttgcggt
     2761 ttgcaccgac cccgccggcg accactacgg ttctgaaccc tgtctgcaac agggcttttt
     2821 tcactttcgc cgccaacaca tcgactaccg catcttgaaa cgcacggcag atgtcgttgc
     2881 gtgtctgctc aggaatgtca tccgccccgt tttccgcgcg cactttctcg acggcggtca
     2941 atacggcggt tttcaaacct gaaaaactca tctgcaaatc gtcggaatga atcatcgggc
     3001 gcggaaaaac aaacgcttcg aacctgcccg attccgcaag ttccgacagt ttcgcaccgc
     3061 ccggatacgg caagcccagc agtttcgccg ttttgtcgaa tgcctcgccc gccgcatcat
     3121 cgacgctctc gcccaaaagc gcgtagtcgc ctatgcccct gaccgccata atctgcgtat
     3181 gcccgcccga aaccaacagc gcgacaaaag gaaagtcggg tttttcctcc gccaacagcg
     3241 gcgacagcag atgtccttcc aaatgatgga cgggaataac aggcttgtcc aacgctaaag
     3301 ccagcgcgtt ggcgtagctc gaacccgcca gcagcgcacc gcccaaaccg ggcccctgcg
     3361 taaaggcaac cgcgtcaatg tcgccatacg atgcgcccgc ctgcgccaaa cagccttccg
     3421 tcaacggaac aaggcggcgg atatggtcgc ggcttgccag ctccggcaca accccgccgt
     3481 attcggcgtg cattgccatc tgcgtgtgca ggcagtgcgc ccgcaatcct cgttcggtgt
     3541 catacagcgc aacgcctgtt tcgtcgcaag aagattcgat tcccaatacc aacatggtct
     3601 gatgccgtta aaaactgaaa aacgtattct agcggatttc ggcacgactg ccgtatccca
     3661 aaaacggaac atgccgtctg aagcccttca gacggcatcg tcgcaccgta tcaaagcgtt
     3721 ccgtaagaat gcagcccgct caaaaacata ttcacgccga taaaagcaaa tgcggttacg
     3781 aacaaaccga taatcgccca ccacgccagc actttgccgc gccaaccggc aaccagccgc
     3841 aagtgcagcc aaacggcgta attgagccag acgatgaacg cccacgtctc tttcggatcc
     3901 caactccaat agcgtcccca agcatctgcc gcccacagcg cacccaaaat ggtggcaatg
     3961 gtaaagaaca gaaagccgac ggcaatcgcc ttatacatca cctcgtcgat caatgccgac
     4021 ggcggcagcc acagttttcc gccttttcct tccgcacgca gggaaaccag ttcggcaata
     4081 ccgagcatcg cggaaatgca aaacgcgccg taaccgataa agtttgccgg aacgtggatt
     4141 ttcatccacc aggactggag cgcgggaatc agcggctgga tggtatgcgc ctcgcgggac
     4201 acgctgtacc acaagacaaa tccaaccacg accgccataa agccgaacac gaagccgccc
     4261 aatttctgta tggcgaactt accttcataa taaagataca tcagcgcggt aatcaccaaa
     4321 aacaggatga acacttcata caggttggaa accggaatat gccccgcatc gggacggagc
     4381 agatagcttt cgtgccaacg taccagcaga ccggtaaagc ctgctacggc agacacccat
     4441 gcaaacacgg ttcccatacc caacagcgtg ttggtcggca cattttttac gcttgccaaa
     4501 accgcgcccg aaatataggc gaacagggcg aagaagacaa aggcgcactg ccacatgatc
     4561 gccgactggc tgctgaggaa ataccgcaac aggaaaatct ctgccgattt gatgttgccg
     4621 ccatacaaac cgacggcggc ataggcaagc aatacgctta aaggaacaaa ccagcgcatc
     4681 ggtttgaaaa accaacccaa aaacacggca ataccggcac ttgcccacaa catgaccgtt
     4741 tcgtaaatgt ccatatgcat accggaacgg gtctgtacga aaaccgtagc cgcaaaaacc
     4801 agcacggcaa atacccaatc ccaaagattc agattgctga tcaaagactt ctgaatcagc
     4861 agctcgtgtt ccggaagggt tttatagtgt tcagtcatga ttcaagtcct tgccgagccg
     4921 ttgcagactc tcgacgtgtt ttggaaattc cttctgcaaa tcccgttcgc tgcgggccga
     4981 agacatggca aaacggattt tgccgtctga aaacaatacc cacgcccgtt tttcgcgcac
     5041 ataaaacatc aataccgtac ccaataccaa cagcaccgag ccgagataga ccaaaagcgc
     5101 acccggggaa cgggtcatct gcaaacccga cgaacgcacc tcggaaaacc catcaagttg
     5161 cagcagcata ggcgcgggat attcggtcaa acccgtgtac gcatccatac tgtgcagcag
     5221 gaaacggttc cgcgcttcat cctgctgcca ttcgggcaag ccgtaccggc gtatggtttc
     5281 atccaaagca gcgttcatca cgccgtaaag catttcgtag aaataaccct gcatcttatc
     5341 ctgctgctct ttcgggatat tggacgtaat aaattcgtcc aatcccaaat agcctttttg
     5401 tgcaaagatg ttcagcgtgt tttccgcagc cagcatgaat tgttcgcgga tttcggcagg
     5461 tgcgtctttg gttgcgtcgg caaccagacg tttgcgccct tccccatctt tcaaaaactc
     5521 acgcaatgcc ataaaggtgt ccgctttcaa ctgcttgtcc aaggggatac gcagccagcg
     5581 gtattgctgc tgcaagccgc tgcgcgtgcc ggtaatccaa aaataatcct gttcctgcaa
     5641 aaccggcagc atatagtttt tatattcgac cgcctgccct gccgcatcac ggatacggta
     5701 aacaatggaa gggccgatat tggtgtattt tttaccttcc tgagtaacgg cgcggacatc
     5761 gttcagcgtg gatttcaggc ttttttcccg ttccgcgccc tcgctcatgt cctccacatt
     5821 catagaagtg aactgatcga actcaagacg atatttgtgt ttgccaattt ccaacggaaa
     5881 ctggtgtatg gatgttgcct tcaacacgac aggctcgcgc gaagcatcac ccaaattcca
     5941 cgccttgaat gtcaaatccg aaccgccgtc ggcaaaactc gcctgataaa tcgtgatgcc
     6001 gtgcaaggtc aaaggatggt tcacgcggat ggtgcgctcg agtttctcac cggttgcctt
     6061 atccgttact tcaatatcac tggcaaaatc gcgcggcata cccgtattgt aaaaatcgat
     6121 atggaatttt ttcagtttga cttcaaaagg caagtcctga accaatatcc cgttgtcggc
     6181 attcaggaaa accacatccg cactctgccc ctcggaaata ttgacgttgc ccctaaatga
     6241 gagattggac gcactcaaaa tactttcggg cttgaaatcc ttggcataaa ccgcctgatt
     6301 gtccggaaca atccgaccgg tcagcatacc cagtttcaac agcaggttac tgtctatcaa
     6361 cccgcccagg caaatgacaa tcaaagcaac atgggcaaag atatagcccc atttgttcat
     6421 cgtgcctttt ttggcggcaa tcagaaccga cccgccctca cggctgacgg tttttccctg
     6481 aaaaccctgc acctccagat aacgtttggc aacctcgggc gcaattttta catccaacag
     6541 cgaagaatgg cgcatcgccg ccagagattt ttctttaacc ttttcccgaa aagacttcat
     6601 ttcgcgccag aacggcggca cattgcgaat caggcacaaa ctggtagaaa ccaccaaaaa
     6661 catcatgata acgacaaacc atgccgaagc atagacgtca tacagtccca gaaaaccaaa
     6721 aatctgcgcc caaaaaggtc cgaatttgac caaataatcc gtctgcggct ggttttgctg
     6781 caacaccgta ccgataaccg atgcaatacc cagcagactg agcaaagcga ctgcaaagcg
     6841 catggagctg aaaaaagcga accacggacg ggaaagaagt gggggagatc tacgggattt
     6901 actcattgtg tgtttattcc gccatcagga atatgggaaa gcagaattgg gcaaacagaa
     6961 aacaacgtcc cgattctact gtcttgatgc tttttatttc aagacaatga agacagcctg
     7021 catcgattcc aacggttgcg attgaaaaaa cttatcgcag aattgcctga agccgtctga
     7081 aaacttttca gacggcctct aaaacagact attgcggaat taacgcaaac cttggataaa
     7141 gttggcgacc gctttcaaat cttcttcaga catacggttt gcaatatctt ccatgatggt
     7201 atttttacgc tgaccggact tgtaggcatt catctgttca acaatatatg cctgatgctg
     7261 accgcccaaa cgcggataag cctgaatttc gcttccgcct cccggcatac ccgcaccgct
     7321 cggaccgtgg caggacatac acgccggcac ttttttatcg ctcaaaccgc cgcgatagat
     7381 tttcgcaccc aattcgggat tttccttagg attggcttca ccggatttgg gctgctgttt
     7441 ggcatagaat gcggatacgt tcaaaatatc ctgatcgctc aaattcatta ccaccggttt
     7501 catcacagct gccgaaccgt gggtgcgttt accgtcgcgg atgccgatag tttgatgata
     7561 gatgtaagca gtatgctgtg ccgccaaacg cggatacatc gcaatgccgc tgttaccgtc
     7621 tgctgcatgg caagccgcac aaaccgttgc ggcaacctgt ttgccttttt ccacgtctgc
     7681 tttgggagag gcggaaaccg caccggcagc caaaacaaag gccaataaag tcaatcgttt
     7741 catggagtgc tcctgattac agcattggat aacgcaacaa tgctcttttt atattcaaat
     7801 acgggatttt tgacccgatt aaaaccgatg attctgtaaa cgtgttattc tatactaaat
     7861 ttacattaaa ttaccactgt gtttcacata aaaccaaccg catatttttg ctgtcggaca
     7921 aacggcggcg gaaaacaagg atatgcccat gaaccttttt caaaacgcca aattcttcac
     7981 gacggtaaac catctcaaag acctgccgga cacaccgctc gaaattgcct ttgtcggcag
     8041 gagcaatgcc ggaaaatcca gtgccatcaa taccctgacc aaccatgtcc gtcttgccta
     8101 cgtttcaaaa acacccggac ggacgcagca tatcaacttc ttcgagctgc agaacggcaa
     8161 ttttatggtc gatttgcccg gctacggtta tgcccaagtc cccgaagcag tacgcgcaca
     8221 ttgggtcaat ctgctcggcg actatctgca acagcgcaaa cagcttatcg ggctggtttt
     8281 gattatggat gcccgccatc ctttaaaaga actcgacatc cgtatgctgg actttttcca
     8341 cacgaccggc agaccggttc acatcctgct gtcaaaagcc gacaaattat ccaaaaacga
     8401 acagataaaa accctgtccc aagtcaaaaa actgctcaaa ccttattccg acaggcaaaa
     8461 catcagcgta cagctgtttt ccagcctgaa aaaacaaggt attgacgagg ccaaccgaac
     8521 tgtcggaagc tggtttgacg cagcagatgc cgccgcttcc tctccagagg aaaactgacc
     8581 ccaattatac ggaaaccgta tttcccccac ttgaccgacc gcaaacattt aaaaaattgc
     8641 cactgccaaa tctaaaatgc cgtctgaaag acttttcaga cggcattttg cggagtcttt
     8701 aaaacagaga atccaactgc tgctgtttgg aaccagtatt actcggaagc accggcgttt
     8761 cctgcatatc ttggcggact tcgtcatccg ccgcctgccg tccgccttct gccgcgcccc
     8821 cgtcatcttc ttttgcccgt cgggaaggtt gcggcgcaat accgctgttg tccagcgtca
     8881 agcccggatc ggttaccata cgttccttca tatagtattc gccattgctg ctgaccacac
     8941 cttcaggcat tttcatcccc ttgccctgct ttcctttcaa cgcaaaacgc atatagtcca
     9001 cccaaaccgg caccgcaatc gtaccgccgt agccgacacg ccccatactc ttaggtttgt
     9061 cgaagccgat atatacggca gtaaccacat cagggttaaa accgacaaac cacgcatcct
     9121 tattgtcatt ggtcgtaccc gttttaccgg caatatccgt tcttcccaac gcagctgccc
     9181 cccttgccgt accaacacgg accacatcct gcataatctt atacataata taggcattgc
     9241 gcggatcgat tgcctgaggc gcattttgcc cggccaccaa aggttgcatt tgggcgcgca
     9301 acctgccgtc tctgtcataa atcttatcga ttacgtgcga agaaacccta tatccgccgt
     9361 tcgcaaatac gctatatgcc tccgccactt tcaacggcgt tgtctcgccc gtacctaaag
     9421 ccatagacag gcttgccggc agctcggacg acctgaagcc gaaacgccgg atatactgtt
     9481 gcgcgtaacc gacaccgata gacatcaaaa tacggatgga aaccatattc ttggaagccg
     9541 tcagagcctg tctcaaagta atgtagccgg aatatctgcc gtctgaattt ttaggtgtcc
     9601 agaccgaacc gttcggccct ttccccggca gggaaatcgg cgcatcgtta accactgtgg
     9661 aagcggtcat ccccttagat aatgccgccg aatagacaaa cggcttaaag gtcgaacccg
     9721 gctgccgcat tgcctgaacg gcacgattga atgttttgct gtgaaaatca taaccgccga
     9781 ccagcgcgcg cacagctccg gtttttgcat ccagcgaaac caaagccccc tgcagcaacg
     9841 gctcttgaac caccgcccaa cgcccgccgt tgtttttgac acggatgacc gcgcccctgc
     9901 ggatacggtc ctcccccatt ttttcattat tgaccgcgcg ggccgcaaaa cccaaggcgc
     9961 gcctgtcaag cgtaacccgc ctgccgccgg gcagctgtat gacgacattt ttctttttag
    10021 tcacatccaa tacgacagcg ggaaccattt tatcgacggt atagagtccc gacagatact
    10081 ggctgacagt ctcctcgaca tcttcactct tactcaaatc gatatagttt tccgcaccgc
    10141 ggtagctgct gccgcgatcg aaattccgta gagccttgcg caatgcctcg gttgccacct
    10201 tctgatgatc ggcgcggacc gtggtataaa ccttaaaacc ctgcgtatag gcatcttcac
    10261 cgtatttctc atacagttcc tgacgcacca tttccgccac atataaagca ctctgatcga
    10321 ttttccgaac aaaccgctcg taatgcagtt cctcattcaa cgcctgatcg cgctgttgca
    10381 cggtaatcat cttctcctcg agcatattgt tcaaaatata cttctggcgc aacttggcac
    10441 gttccggatt aacaatcgga ttataggcag acggagcctt gggcagtccc gcaagcatgg
    10501 cggcttccgc caaagtcaaa tctcggacat tcttattgaa atagatttgc gcggcagatg
    10561 caaaaccata ggcgcgctga ccgaggtaaa tctgattgaa atacaactcg aggattttgt
    10621 ctttgcttaa agactgctcg attttatagg caagcaacac ctcattgaat ttgcgtgtga
    10681 acgttttttc actgctcaaa taaaaatttt tcgccacctg ctgcgtaatc gtactcgcac
    10741 ccgactgcac gctgccggac acgacattgc cgacggcagc gcgggcaaca ccccaaacat
    10801 ccacccccca atgccggtaa aagcgtttgt cctcggcggc gataaccgca ttccgcaaca
    10861 cctctgggaa atcgccgatt tttgtaaatt cgcgccgctg ctccccatac ataccgatga
    10921 cttccccatc cgccgaataa atagtcaacg gcattttagg ctggtaatgc tgcaaagaat
    10981 ccaaagacgg cagtttcgga tacgttacca aaatagcaat ggcaaccaaa cccactccaa
    11041 atacacaaaa cccaaaaacc aaaccaaaac aagtcgttaa aatcttttta atcatagctg
    11101 aataataatt taccattatt ggtattaaat aaagtaaaat agcaaccgat ttctacaaag
    11161 cacggtttca atgtgcaaag aacaaggaat ccattacgga taccgaaacg gttactcact
    11221 gtacaaataa agcaggaact ttcatcatgc gcttgtttaa aagcttgaaa aaccctaaaa
    11281 aaacagatgc caagctccct aaaaaatctt cgggactcaa taaccgcgcg gcaatcggca
    11341 tcgatatcga ccagcattcc atcaaaatgg tccaattgtc aggacgtagt ttaaaccaaa
    11401 ttcaattgga aaaatacgtc attgccaaat taccaaagaa tatcattcaa ggcaataaag
    11461 tccaaaatta cgatcaactt gttacatatt tgcaacaagc ctatgccaaa ctgggtactt
    11521 cgtgcaaaaa catcatcgcg tccgtcccgc aaaatctggc aaccatcgaa caattgacct
    11581 acacagacaa agatgcagaa ttagacctgc aggggttcgt ggagtcctcc atctccgaag
    11641 tcagctcgat atcgctcgaa gaagccaatt acgactatca ggtcttgtcc caatcggccg
    11701 ccggcgaagc tgtgttggcc gtcgcatcga gaaaggatga aatcgaaccc ctgattgacg
    11761 cattcaacgc cgccggtatg aaattatccg cgcttgatgt ggacattttc ggacaataca
    11821 acgcctacgc gctatggata aaccatttcg cccccgagct tgcagccgaa aaagtcgcca
    11881 ttttcggcgt atatgccgca cagacctacg ccttggtcat ccaagacgga aaaatcctat
    11941 acaaacagga aacctccgtc agcgaagaac agctcaacca actcatccag cgcacctatc
    12001 aggtaacaga agaaaaagcg gaagaaatca tcaactcccc gcaaaaacct tccgattacc
    12061 aagaaagcgt ggcaaactat ttcaaccagc agattaccca agaaatacaa agggtcttgc
    12121 agttttatta caccacgcag accgcagacg atatgaccga catcaagcat atcctgctga
    12181 ccggggaagc ggcgcgccag gaaggcatcg cccaaaccgt cgcctcacaa accaatgcag
    12241 atgtacaatg cgtccatccc gcgcgttatt ttgcggacaa cctcaaaaca gacaaacaac
    12301 aattcgaact tgatgcgccg acactgacca gggcgttcgg tttggcggta cggggattat
    12361 aattatgaac aatttaatca aaatcaacct cctcccctac agggaagaga tgaacaagcg
    12421 caaacagcag cagtttaaaa cgctgatgta cggtgccgtg ctgacgggcg ttgccgccgt
    12481 tgcggcaacc tacctgttta tcgacaatat gatcaataac cagtcggaaa gaaacacgct
    12541 gctggaaacc tccatcgcac acttggatac cgagctgtcg gaaatacaaa agctcaaaca
    12601 ggaaaaagat gccttcctga ttaagaaaaa caaaatcgag gagctccagc tcaaacgcct
    12661 ccaagccgca aaaatcctcg acagcctgaa tgaggccgtc cccggaagca cctacctgac
    12721 ctcgctggat gccgttaccg ccgactctta tcggctcagc ggcaggacat ccagcgacaa
    12781 ccgcgttgcc gccatgatga gggcgatgcc caataccggc atattcaagc aacccgaatt
    12841 gttaagcatc aagaaaaaca attcgcatca agaatttacc cttcaggcaa cattacaacc
    12901 catcgtaaag gcggccgaat ccaaagagaa tccggcttcg ggaaacgcac aggaggcaaa
    12961 ctgaatggct tctaaatcat ctaaaaccaa cttggatctc aacaaccttc acctgctcaa
    13021 ccttcctgcc aggcttttta tcgccctgct ggccgttgcc gccgtgctgg ggctcggtta
    13081 tgccggattg ttcaaaagcc agatggaatc ccttgaggaa tacgaagcaa aagaaaccga
    13141 actgaaaaac acctacaaac agaaaagtat cgacgcggcc agcctgaaca acctgaggga
    13201 cgaacttgcc tcaatccgct ctgccttcga tatcatgttg aaacagctgc cgacagatgc
    13261 agaaattccc aatctggttc aagagcttca tcaggcaggt tcgagcaacg gtctgcgctt
    13321 ggacagcgtt atgccccaac ctcccgtaga tgacggcccc atcaaaagat taccctattc
    13381 catttccatt accggaaatt acgaacagat cagccaattt acccgcgatg tcggcagcct
    13441 ctcccgaatc attacccttg agtcgctgaa aatcgcccaa tctccggaaa acggcggcaa
    13501 tcctgacggc aagagcagca tcctgaacct cagcgccatt gccaccacct accaagcaaa
    13561 atccgtagaa gagcttgccg cagaagcggc acaaaatgcc gagcaaaaat aacttacgtt
    13621 agggaaacca tgaaacacta tgccttactc atcagctttc tggctctctc cgcgtgttcc
    13681 caaggttctg aggacctaaa cgaatggatg gcacaaacgc gacgcgaagc caaagcagaa
    13741 atcatacctt tccaagcacc taccctgccg gttgcgccgg tatacagccc gccgcagctt
    13801 acagggccga acgcattcga cttccgccgc atggaaaccg acaaaaaagg ggaaaatgcc
    13861 cccgacacca agcgtattaa agaaacgctg gaaaaattca gtttggaaaa tatgcgttat
    13921 gtcggcattt tgaagtccgg acagaaagtc tccggcttca tcgaggctga aggttatgtc
    13981 tacactgtcg gtgtcggcaa ctatttggga caaaactacg gtagaatcga aagcattacc
    14041 gacgacagca tcgtcctgaa cgagctgata gaagacagca cgggcaactg ggtttcccgt
    14101 aaagcagaac tgctgttgaa ttcttccgac aaaaacaccg aacaagcggc agcacctgcc
    14161 gcagaacaaa attaagaaga ggattactcc attatgaata ccaaactgac aaaaatcatt
    14221 tccggtctct ttgtcgcaac cgccgccttt cagacagcat cggcaggaaa cattacagac
    14281 atcaaagttt cctccctgcc caacaaacag aaaatcgtca aagtcagctt tgacaaagag
    14341 attgtcaacc cgaccggctt cgtaacctcc tcaccggccc gcatcgcctt ggactttgaa
    14401 caaaccggca tttccatgga tcaacaggta ctcgaatatg ccgatcctct gttgagcaaa
    14461 atcagtgccg cacaaaacag cagccgtgcg cgtctggttc tgaatctgaa caaaccgggc
    14521 caatacaata ccgaagtacg cgggaacaaa gtttggatat tcattaacga atcggacgat
    14581 accgtgtccg cccccgcacg ccccgccgta aaagccgcgc ctgccgcacc ggcaaaacaa
    14641 caggctgccg caccgtctac caagtccgca gtatccgtat ccgaaccctt taccccggca
    14701 aaacaacagg ctgccgcacc gtttaccgag tccgtagtat ccgtatccgc accgttcagc
    14761 ccggcaaaac aacaggcggc ggcatcagca aaacaacagg cggcagcacc agcaaaacaa
    14821 caggcggcag caccagcaaa acaacaggcg gcagcaccag caaaacaaac caatatcgat
    14881 ttccgcaaag acggcaaaaa tgccggcatt atcgaattgg ctgcattggg ctttgccggg
    14941 cagcccgaca tcagccaaca gcacgaccac atcatcgtta cgctgaaaaa ccataccctg
    15001 ccgaccacgc tccaacgcag tttggatgtg gcagacttta aaacaccggt tcaaaaggtt
    15061 acgctgaaac gcctcaataa cgacacccag ctgattatca caacagccgg caactgggaa
    15121 ctcgtcaaca aatccgccgc gcccggatac tttaccttcc aagtcctgcc gaaaaaacaa
    15181 aacctcgagt caggcggcgt gaacaatgcg cccaaaacct tcacaggccg gaaaatctcc
    15241 cttgacttcc aagatgtcga aatccgcacc atcctgcaga ttttggcaaa agaatccgga
    15301 atgaacattg ttgccagcga ctccgtcaac ggcaaaatga ccctctccct caaagacgta
    15361 ccttgggatc aggctttgga tttggttatg caggcacgca acctcgatat gcgccaacaa
    15421 gggaacatcg tcaacatcgc accccgcgac gagctgcttg ccaaagacaa agccttctta
    15481 caggcggaaa aagacattgc cgatctgggc gcgctgtatt cacaaaactt ccaattgaaa
    15541 tacaaaaatg tggaagaatt ccgcagcatc ctgcgtttgg acaatgccga cacaaccgga
    15601 aaccgcaata cgcttgtcag cggcaggggc agcgtgctga tcgatcccgc caccaatacc
    15661 ctgattgtta ccgatacccg cagcgtcatc gaaaaattcc gcaaactgat tgacgaattg
    15721 gacgtacccg cgcaacaagt gatgattgag gcgcgtatcg tcgaagcggc agacggcttc
    15781 tcgcgcgatt tgggcgttaa attcggcgcg acaggcaaga aaaagctgaa aaatgataca
    15841 agcgcattcg gctggggggt aaactccggc ttcggcggcg acgataaatg gggggccgaa
    15901 accaaaatca acctgccgat taccgctgcc gcaaacagca tttcgctggt gcgcgcgatt
    15961 tcctccggtg ccttgaattt ggaattgtcc gcatccgaat cgctttcaaa aaccaaaacg
    16021 cttgccaatc cgcgcgtgct gacccaaaac cgcaaagagg ccaaaatcga atctggttac
    16081 gaaattcctt tcaccgtaac ctcaatcgcg aacggcggca gcagcacgaa cacggaactc
    16141 aaaaaagccg tcttggggct gaccgttacg ccgaacatca cgcccgacgg ccaaatcatt
    16201 atgaccgtca aaatcaacaa ggactcgcct gcgcaatgtg cctccggtaa tcagacgatt
    16261 ctgtgtattt cgaccaaaaa cctgaatacg caggctatgg ttgaaaacgg cggcacattg
    16321 attgtcggcg gtatttatga agaagacaac ggtaatacgc tgaccaaagt ccccctgttg
    16381 ggcgacatcc ccgttatcgg caacctcttt aaaacacgcg ggaaaaaaac cgaccgccgc
    16441 gaactgctga ttttcattac cccgaggatt atgggtacgg ccggcaacag cctgcgctat
    16501 tgatgcgtca aaataagggc atatgtttta cggcatatgc cctttcttta tgctttttgc
    16561 cgcgaccgaa atgccgtcat tcccgcgcag gcgggaatcc aacttgtccg gtttcggttg
    16621 tttttcgtct cgtaactttt gagccgtcat tcccgcgaaa gcggaaatcc aatcc
//
LOCUS       DAXHSJ010000027        16363 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000027 DAXHSJ010000000
VERSION     DAXHSJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16363
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.027"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            1..149
                     /locus_tag="ACFM8U_000665"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACFM8U_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(159..1268)
                     /gene="mltB"
                     /locus_tag="ACFM8U_000666"
     CDS             complement(159..1268)
                     /gene="mltB"
                     /locus_tag="ACFM8U_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC6333945.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1177..1428)
                     /locus_tag="ACFM8U_000667"
     CDS             complement(1177..1428)
                     /locus_tag="ACFM8U_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333946.1"
                     /translation="MPLDGIFGGGRQISGGAKGNARHSAKPPIAADTAFRAAPLRRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            complement(1469..2896)
                     /locus_tag="ACFM8U_000668"
     CDS             complement(1469..2896)
                     /locus_tag="ACFM8U_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6333947.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKIIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            3081..6779
                     /gene="mfd"
                     /locus_tag="ACFM8U_000669"
     CDS             3081..6779
                     /gene="mfd"
                     /locus_tag="ACFM8U_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC6333948.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDNEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSSDVFAGRLKNYGQVLP
                     DIFGVEHTLPNVAVNRQADEPLQALKDFQTAFKGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRIHNRRKKHAAVSDDLLRDLAEINIG
                     DPVVHEEHGIGRYMGLITMNLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     NIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINELDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMVDGTVDIVIGTHK
                     LVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIE
                     SHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYRLAGADKLRF
                     TAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            6928..7311
                     /gene="panD"
                     /locus_tag="ACFM8U_000670"
     CDS             6928..7311
                     /gene="panD"
                     /locus_tag="ACFM8U_000670"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC6333949.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7333..8175
                     /gene="kdsA"
                     /locus_tag="ACFM8U_000671"
     CDS             7333..8175
                     /gene="kdsA"
                     /locus_tag="ACFM8U_000671"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC6333950.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCAHYAEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8190..8630
                     /locus_tag="ACFM8U_000672"
     CDS             8190..8630
                     /locus_tag="ACFM8U_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC6333951.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAALVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAILTRLQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAVLSWRLSGKDAV"
     gene            8678..9964
                     /gene="eno"
                     /locus_tag="ACFM8U_000673"
     CDS             8678..9964
                     /gene="eno"
                     /locus_tag="ACFM8U_000673"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC6333952.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGNKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            9979..10257
                     /gene="ftsB"
                     /locus_tag="ACFM8U_000674"
     CDS             9979..10257
                     /gene="ftsB"
                     /locus_tag="ACFM8U_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC6333953.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10298..10588)
                     /gene="yfaE"
                     /locus_tag="ACFM8U_000675"
     CDS             complement(10298..10588)
                     /gene="yfaE"
                     /locus_tag="ACFM8U_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC6333954.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(10786..11919)
                     /gene="nrdB"
                     /locus_tag="ACFM8U_000676"
     CDS             complement(10786..11919)
                     /gene="nrdB"
                     /locus_tag="ACFM8U_000676"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC6333955.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGMHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(12133..13113)
                     /locus_tag="ACFM8U_000677"
     CDS             complement(12133..13113)
                     /locus_tag="ACFM8U_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC6333956.1"
                     /translation="MTAARAGLVRFNYLKVKQAVQSKQIKGFKLWKSYDEQLEILRSR
                     GLQIDDEKKALGYLRTIGYYRLSGYFYSFRQFAPESSKSRLDEFITDTRFEDVKSLYM
                     FDKKLRQLALDALERIEVALRVNIAHTLGRHDPLAYKDSKYSATGFDHAEWLDRYNRL
                     VEREKKTGFVKHHLEHYADLPVWVACEVWDFGTMSMLYKGMLNKDKDSIAKLYRLKDG
                     KHLQTHLHAFNFIRNVSAHHSRLWNRDITFKASLKGLSDAEWKSLPINKPFVYFCLMK
                     RMLDVICPRTEWGNRLLALLDEFPDVANEAVSLRDMGLENNPRDWRLWQG"
     gene            complement(13079..15358)
                     /gene="nrdA"
                     /locus_tag="ACFM8U_000678"
     CDS             complement(13079..15358)
                     /gene="nrdA"
                     /locus_tag="ACFM8U_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC6333957.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQAPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            15887..16198
                     /locus_tag="ACFM8U_000679"
     CDS             15887..16198
                     /locus_tag="ACFM8U_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC6333958.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         3999 a         4474 c         4050 g         3840 t
ORIGIN      
        1 atgcaaaaat gccgtccgaa ccgaaacgtg gttcagacgg cattttatag tggattaaca
       61 aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcaaggcaa
      121 cgccgtactg gtttaaattt aatccactat aaaaaacatt acaatcccgg gccgccaagc
      181 gaattggcaa tgtccctgac cgccgttaca tacatccgac tgtgattgta ctgccatacc
      241 gtataaaaat tgttcaagcc caaataatat tcaaacacgc cgggtgcggt ttccagtttg
      301 aacaaaaccg ccttttcatc atctgcaagc tcttcgccgg ggatgatgcc gtacgccttt
      361 aaatccgcca ccgtccgcgt cagggcggtt ttttcgccaa tgattgcctg aacatccgca
      421 cccggcgcca atgttgcaga caccagtatt ttcccgcccg tgcgccaacc gtgctgcttc
      481 atataattgg caaccgatgc cgcgacatcg ccgatgttgc cccatatgtc ccgatgtccg
      541 tccccgtcat aatccaccgc ccatttccgg tagctcgaag gcataaattg cggcatcccc
      601 attgcgcccg catagctgcc tttaaaggcg aaaacatcgc cgccttcttc ttttgccagc
      661 tttaaaagct cgaccaattc tttttggaaa aacccggcgc ggcgggggta atcaaagcct
      721 aaggtcgcca atgcgtccgc cacacggaaa ctgcccgtat ttttgccgta attcgtttca
      781 atcccgataa ccgccacgat aagttcggca ggcacgccgt atttttgcgc cacatcatcg
      841 ataagcgcgc ggttttccgc ataaaaccgg cgcgcgccgc gaaatttcgc cttgcccgaa
      901 tttcccgtgc ggaacacata ccacggacgc gatgtggagg ggcggtgcat aatcttgacg
      961 atgtccgcct tgtaagccgc tttgtcaaaa aaatcctgcc attccgcccg ggaaaaatcc
     1021 cctttcccga cttcatcgtc cacaaaacgg cggacatttg cattggcggc aaacccgctg
     1081 tcggataccg gtacggctgc cgcgtcaaat acggctgccg cgtcaaacgc ggggcggctt
     1141 tcttttttca tttcatccgc gcggggggct tgggcttcat ttgcccgggg cgggcgtgcc
     1201 tccatcgccg tacaggcaga caaagccgcc aaacaaattg ccagcggcag tatttttctc
     1261 tttttcataa atatgttccg aacaaatagg gtaagtggga aagcggcgca agggcgcggc
     1321 gcgaaatgcg gtatccgccg caatcggcgg ctttgcagaa tgccgcgcgt tgccttttgc
     1381 gccgcccgaa atctgcctgc cgccgccgaa aatgccgtct aaaggcactt cccctttcag
     1441 acggcattgc ctgcgccgta tttgcccgct atccgcaata tcggcagtcc aatatgtctt
     1501 tgcggatatc gttcagcagg caggctgccg cgtcttcgtg ttccgcctgt acgaagacaa
     1561 agagtcgggc gatgattttg ccgataaaga ctttttgcag ggcgcaggca tcggtcaggg
     1621 tgtgttcctg caggaagctg cggatgtctt cgggcaaggt gtagtcgcgg gcaacggcgg
     1681 caaagcgggc atcggtttgc aggcggtcga gcagggtttg gtttttgtcc aacttcatgc
     1741 ctgcttcttt ttcttcggcg gtggcgcgga caaactggtc gtaaatttcg gcataaagca
     1801 tatcgttcgg gccttcaaaa atcgtgaagg gacggatgtc gatggcgata ttgccggcgg
     1861 tgtgtccgcg ttcaaaaccc ttcgcgccca agagtttttg caacatttgc gcggcggcat
     1921 aagtgtattc cgtggcgagg gttttgacga tgttcgcctc catcagttga tgggcgacgg
     1981 gtgcaacggg cgaaacggaa tggcagacgt agcggtaaag aatctcggaa acctgatggc
     2041 ggcgccggat ttcgcggcgt tcgtaatcga cgaatctgat gtcgttgcgg acgtatcgtt
     2101 ccaggttttc aaggatgtat tccataatgc cgtgcgtcat gccgatcagt tgcaggcggc
     2161 tgcggataaa gatgttttgg aacgcgcgca aaccggcagc gtcgctctgg gagagtttca
     2221 tcacggcggt tgcaggcatt tcggcatcga tgcggttgac ggcgtaacga acggcgcgca
     2281 agccttcgga tgcgagggtt tcgcagcgga tgtatgtttt ggggacgagc agcaggtcga
     2341 tgattttggc gagtttgccg tttttgcgct ctttggcggc aacgaggagg aagtcgcttt
     2401 gcgagttacc ctgccagtat ttcgcggcgt tgacgtaaat ggtttgtccg tcggtatatt
     2461 cgtagtaaga ctgcatttcg cgtgcaatcg ccgcgccgga ggtttcgggt tcggtaacgc
     2521 ccaaaccgcc gccctcgcct ttgaaaacca tgtccaaacc ctgtgcgatt tgcgcttcat
     2581 cgccgaactc ttgcagtggc tgcaacacca gcgcgccttc gatgccggta cgcagcgtaa
     2641 cgggcacgcc gtaatgcccc gcaatccgca agacttcctg aatttcaaac tggctgccct
     2701 tgcggccgcc gtgttttttg tcgaggaagg gcaacagcaa acccgcctgc ttcaaggcaa
     2761 gccatttgtc ttcgggcagg tatcgcatca ggtcgatacc gtctgaaaaa atgcggcgga
     2821 atgcggattc gatgtgcttt aaaaaagcag ccgtgtccat agttgacggc tgcgcgctcg
     2881 gttcggtgtg tatcatcggc ttcctctgtc ggttcccatt aatcggcggc cggtcaaacc
     2941 gcctgccaca gtttagagtt gattttctaa actttaccac aaagtgcgcc gggcaacaat
     3001 ccgccgacct ttcagacggc atcgcgcccc ctcccgtgct aaaatgaccg tttgcatcac
     3061 tgtccgccga ttgccgcact atgacctacc ccatccccaa accccgtgaa aaatcccgtt
     3121 ggtccaatct ttcgcaaggc tcgctgccct tggctttggc gcgttatctg ccgcataagc
     3181 ggctcaaggt cgtgctgacc caagatgcgg aacaggcgct acgccttcag acggcatggc
     3241 ggtttttccg tccgcacgac acggcggtgt tcctgccgga ctgggaaacg ctgccttacg
     3301 agcgtttttc gccgcatcag gatttggtgt cggaacggct gtcggcgttg tggcagatta
     3361 agagcggcgc ggcggacgtg ttgttcgtgc cggttgccac ggcgatgcag aagctgccgc
     3421 ccgtgccgtt tctggcaggg cgcacgtttt ggctgaaaac agggcagact ttggatatag
     3481 gccgtctgaa aaccgatttg gtagatgcgg gctacaacca tgtttcccat gttgttgcgg
     3541 cgggcgagtt tgccgtgcgc ggcggcattg tggatttgtt cccgatgggc agcgaaatgc
     3601 cgtaccgcat cgatttgttc gacgatgaaa tcgacagcat caaaaccttt gacaccgaga
     3661 cgcagcgcac catctccccc gtttccgaaa tccgtctgct gccggcgcac gagttcccca
     3721 ccgacaacga ggcgcaaaaa atcttccgca gccgttttcg cgaggaagtc gatggcaatc
     3781 cgaacgatgc cgctgtgtac aaagccgtca gcaacggtca tttcggcgcg ggcgtggaat
     3841 actacctgcc gctgtttttt gaaaacgagt tggaaacgct gtttgactat atcggcgaag
     3901 atgcgctgtt tgtctcttta ggcgatgttc atgccgaggc aaaccgtttt tggagcgatg
     3961 tcaaatcgcg ttacgcgatg gcgcagggcg acgaaaccta tccgcctttg cttccacagc
     4021 atttgtatct ctcctcagat gtttttgcag gccgtctgaa aaattatggt caggtattgc
     4081 cggatatttt tggtgttgaa catacgttgc caaacgtggc cgtcaaccgc caagcggatg
     4141 aaccgttgca ggcattgaaa gattttcaga cggcctttaa aggacggatt ttgttgtgcg
     4201 ccgaaagttt gggacgacgc gaaaccatgc tcggtttctt gcagcaaaac ggtttgaaag
     4261 ccaaacccgt gtccgactgg cagggctttt tatcggcaca cgagccgctg atgattacag
     4321 tggcgccgtt ggcatacggg ttcaaactgg gcggactgca atcgccgaac caacagcaaa
     4381 ctacttccgc ctccgaggga gaaggcgatg cggttaccga ccaaaccgaa ttttccgcat
     4441 ccgcaacaaa ccctctccct agccctctcc ctcaggagag ggaacaaagt gcagccgtcg
     4501 tttcagacag tctgaaagca gccgccgttt caaccgaaag cagcctgccc cccggtaaaa
     4561 gtaatctgca cgggcaaatc caacagcaac ctgccccctc ccccgtgggg gagggttggg
     4621 gagaaggcaa agcagttgcc gctcaaagtg ccatcgccgt catcaccgaa tccgatctct
     4681 accaatacgt cgcccgttcg cgcatccaca accgccgcaa gaaacacgcc gccgtttcag
     4741 acgacctgtt gcgcgacctt gccgaaatca acatcggcga ccccgtcgtg cacgaggaac
     4801 acggcatcgg ccgatatatg ggcttgataa caatgaactt gggcggcgaa accaacgaaa
     4861 tgatgttgct cgaatatgcg ggcgaagcgc agctttatgt gcctgtttca caactgcatt
     4921 taatcagccg ctactccggt caggcgcatg aaaacattgc cctgcacaag ctcggcagtg
     4981 gcgcgtggaa caaggcgaag cgcaaagccg ccgaaaaggc gcgcgatacc gccgccgagt
     5041 tgcttaacct ttacgcccga cgcgctgccc aatcgggaca caagtttgaa atcaacgagt
     5101 tggattatca ggcgtttgct gacggcttcg gctacgagga aaccgaagac caggccgccg
     5161 ccatcgccgc cgtgattaaa gatttgacgc aagcgaagcc gatggatcgc cttgtgtgcg
     5221 gcgatgtcgg tttcggcaaa accgaagtcg ccctgcgcgc cgcgtttgtg gcggtgatgg
     5281 gtggcaaaca ggtcgccgta ctcgccccaa ccacgcttct ggtcgagcag cacgcgcaaa
     5341 actttgccga ccgtttcgcc gatttccccg tgaaagtcgc cagcctttcg cgtttcaaca
     5401 acagcaaagc caccaaagcc gcgctcgaag gcatggtgga cggcacggtc gatattgtta
     5461 tcggcacgca caaattggtg caggacgaca tcaaattcaa aaacttaggt ttagtgatta
     5521 tcgatgaaga acaccgcttc ggcgtgcgtc agaaagaaca gctcaaacgc ctgcgcgcca
     5581 atgtcgacat ccttaccatg accgccacgc cgattccgcg caccctcagc atggcactcg
     5641 aaggactgcg cgacttttcg ctgattacca ccgcgcccag ccgccgcctt gccgtcaaaa
     5701 cctttgtcaa accctttagc gaaggcagtg tgcgcgaagc agtattgcgc gaactcaaac
     5761 gcggcgggca agtgtttttc ctgcataatg aagtggatac catcgaaaac atgcgcgagc
     5821 ggctggaaac cctattgccc gaagcccgca tcggcgtggc gcacggacaa ctgcgcgagc
     5881 gcgagctgga gcaagtgatg cgcgactttt tgcagcaacg atttaacgtg ttgctctgtt
     5941 ccaccatcat cgaaaccggc atcgacatcc ccaacgccaa taccatcatc atcaaccgcg
     6001 ccgataagtt cgggctggcg caattgcacc agcttcgcgg gcgcgtcggc cgcagccacc
     6061 accaagccta cgcctacctg ctcacgcccg aatacatcac caaagacgca gaaaaacgtc
     6121 tcgatgccat cgcggcggca gacgaactcg gcgcaggttt caccctcgcc atgcaggatt
     6181 tggaaatccg cggcgcaggc gaaatcctcg gcgaagggca gtccggcgaa atgatacagg
     6241 tcggcttcac gctctacacc gaaatgctca aacaagccgt gcgcgacctc aaaaaaggcc
     6301 gccagcccga cctcgacgca ccgctgggca tcaccaccga aatcaaactg cacagccccg
     6361 ctctgctgcc cgaaagctac tgccccgaca tccacgagcg tctcgtcctc tacaaacgcc
     6421 tcgccgtctg cgaaaccgtg cagcaaatca acgccataca cgaagaactc gtcgaccgct
     6481 tcggactgcc cgaacaaccc gtcaaaaccc ttatcgaaag ccaccacctg cggcttatgg
     6541 caaaagaatt gggtatcgat gccattgatg cggccggcga agcggtaacg gtaacctttg
     6601 gtaaaaacaa taatgtcgat ccaaccgaaa tcatcctgct gattcagaac gacaaaaaat
     6661 accgccttgc cggcgccgat aagctgcggt ttaccgcaga gatggaaaat atcgaggtca
     6721 gaatcaacac cgtaaaaaac gttttaaaaa ccttgcaaaa cagatgcctg cccaaataaa
     6781 gccgacaccg caatgccgtc tgaaacaccg ttttccttgt ccgaaagccg ccattatgaa
     6841 tttgaaggaa actccactat aatacggcat tcagatttcc agacggcatc gcgcccgtca
     6901 aaccgcacac aaaccaaaag gaaatacatg ttccgtacta tgcttggcgg aaaaatccac
     6961 cgcgccaccg ttaccgaagc cgatttaaac tatgtcggca gcattaccgt cgatcaagac
     7021 ctgttagacg cggcaggcat ctaccccaac gaaaaagtcg ccatcgtcaa caacaacaac
     7081 ggcgaacgtt ttgaaaccta taccattgca gggaaacgcg gcagcggcgt gatttgtctg
     7141 aacggtgctg cggcaaggct ggtgcagaaa ggcgatatcg tcatcatcat gtcttacgtc
     7201 caactctccg aacccgaaat cgccgcgcat gaacccaaag tcgtcttggt agacggaaac
     7261 aacaaaatcc gcgacatcat ctcctacgag ccgccgcaca ccgtgctgta accctgaaaa
     7321 cggacattga ccatggatat taaaattaac ggcattaccg tcggtaacga cgctcctttt
     7381 gtactgttcg gcggcatcaa cgttttagaa gatttggatt ccaccctcca aacctgcgca
     7441 cattacgccg aagttacccg aaaactcggt attccctata tctttaaagc ctctttcgac
     7501 aaggcaaacc gttcctccat ccattcttat cgcggcgtag gcttggaaga aggcttaaag
     7561 atttttgaaa aagtcaaagc agagttcggc atccccgtca ttaccgacgt acacgaaccc
     7621 catcagtgcc aacccgtcgc cgaagtgtgc gatgtcatcc agcttcccgc ctttcttgcg
     7681 cggcagaccg atttggtggc cgcaatggcg gaaactggca atgtcatcaa catcaaaaaa
     7741 ccccagttcc tcagcccctc tcaaatgaaa aacatcgtgg aaaaattccg cgaagccggc
     7801 aacgggaagc tgattttatg cgaacgcggc agcagcttcg gctacgacaa cctcgttgtc
     7861 gatatgctcg gtttcggcgt gatgaaacaa acctgcggca acctgccggt tattttcgac
     7921 gttacccatt ccctgcaaac ccgcgatgcc ggttctgccg catccggcgg tcgtcgcgca
     7981 caggctttgg atttggcact tgcaggcatg gcaacccgcc ttgccggcct gttcctcgaa
     8041 tcgcaccccg atccgaaact ggcaaaatgc gacggcccca gcgcgctgcc gttgcacctt
     8101 ttagaagatt ttttaatccg catcaaagca ttggacgatt taatcaaatc acaaccgatt
     8161 ttaacaatcg agtaacacgg tttcgcctta tgatgcagac tttccgaaaa atcagcctgt
     8221 atgccgcaac cttgtggctc ggtatgcaga ttatggcggg ttatatcgcc gcactggtgc
     8281 tgttcaaaat gctgcccaaa atgcaggcgg gcgaaattgc cggcgtattg ttcgacatcc
     8341 tctcttggag cgggcttgcc gtttggggcg cggtgttggc tgctgccttt gccatcctga
     8401 cccgactgca aaccgccctg ctgctttttt tattgtccgc ccttgccgcc aaccaatttt
     8461 tggttacccc cgttatcgag gcactgaaat acgggcatga aaattggctg ttgtcgtttg
     8521 taggcggatc cttcggaatg tggcacggta tttccagcat gattttcatg gcaaccgccc
     8581 tactttcagc agttttaagt tggcggcttt ccggcaaaga tgccgtctga agccctccca
     8641 tttttttacc tcccttcact tcacttggag aacattcatg agcgcaatcg ttgatatttt
     8701 cgcccgcgaa attttggact cacgcggcaa ccccacagtc gagtgtgatg tattgctcga
     8761 atccggcgta atgggacgcg cagccgtacc gagcggcgcg tccaccggtc aaaaagaggc
     8821 tttggaactt cgcgacggca acaaatcccg ctattccggc aaaggcgtat tgaaggcggt
     8881 cgaacacgtc aacaaccaaa tcgcccaagc cctcatcggt atcgatgcca acgagcaatc
     8941 ttatatcgac caaatcatga tcgaattgga cggtactgaa aacaaaggca atttgggtgc
     9001 gaatgcgact ttggcggttt ctatggcggt tgcacgcgcc gctgccgaag actcaggcct
     9061 gccgctttac cgctacttgg gcggcgcagg cccgatgtcc ctgcccgtac cgatgatgaa
     9121 cgtcatcaac ggcggcgaac acgccaacaa cagcctgaac atccaagagt ttatgattat
     9181 gcccgtcggc gcaaaatctt tccgcgaagc gttgcgctgc ggtgcggaaa ttttccacgc
     9241 cttgaaaaaa ctgtgcgaca gcaaaggctt cccgaccaca gtcggcgacg aaggcggttt
     9301 cgcccccaac ctgaacagcc acaaagaagc cctgcaactg atggtcgagg cgaccgaagc
     9361 cgccggctac aaagcgggcg aagacgtatt attcgcattg gactgcgcgt ccagcgagtt
     9421 ctacaaagac ggcaaatacc acttggaagc cgaaggccgc tcctacacca acgcggaatt
     9481 tgccgaatat ctggaaagtc tggtcaacga gttccccatc atctccatcg aagacggcat
     9541 ggatgaaaat gactgggaag gctggaaact gctgaccgaa aaactgggtg gcaaagtcca
     9601 actcgttggc gacgacctct tcgttaccaa cccgaaaatc cttgccgaag gcattgaaaa
     9661 aggcgtggca aacgcactat tggtcaaagt caaccaaatc ggtactttga gcgagaccct
     9721 gaaagccgtc gacttagcca aacgcaaccg ctacgccagc gtaatgagcc accgctccgg
     9781 cgaaaccgaa gacagcacca ttgccgactt ggcagtcgcc accaactgta tgcagatcaa
     9841 aaccggttct ttgagccgtt ccgaccgcat ggcaaaatac aaccaactgc tgcgtatcga
     9901 ggaagaattg gcggaagccg ccgactaccc cggcaaagcc gcattctacc aactgggcaa
     9961 ataagaaaag gttaaggtat gaagtgggta actgtcgttt tatccttcgc acttgtctgt
    10021 tgccaataca gcctctggtt cggcaaaggc agcatcggac gcaacagcag tctgagagaa
    10081 cagattgccg ttcaagaaga aaaaaaccag acactcgccc tacgcaatca ttcccttgcc
    10141 gccgaagtct atgatttgga aaacggtcaa gaagccattt cggaaatcgc ccgggtagaa
    10201 ctgggttata tccaagacgg tgaaaccttt taccgactca tcaggcataa ccggtaatac
    10261 cgtcaaaaag ccgtccgaac caatgttcgg acggctttta tttcaacaaa ctgtcagaca
    10321 gcccctcatc ctcccccgac aaaccgcaat ccagcctgac atccccctcg acgcaacagc
    10381 agcacggcag tatctcgtcc cgccccaaaa aagccaaagg cggctcccga taagtaacgc
    10441 ttccctccaa aatcttcact cggcacgatc cgcaatatcc gcttcggcac tgatattcca
    10501 ccatatgccc cgtccgttcc aagccttcca acagggtctc gccctccaag agctcaaaaa
    10561 aacccttatt cgtaccaatg cgcgccattt ccgaccaatc aaaaatatag tggattaaat
    10621 ttaaaccagt acggcgttgc ctcgccttgt ccggattttt gttaatccac tataatccac
    10681 tataaaagga acaataaccg atcctacccg ctgtttttcc catcatacaa catacaaatg
    10741 ccgtctgaaa catccggctt cagacggcat tttttcaaaa aacatttaca actcaaaatc
    10801 gcccaaatca tccgtattca cttcagaatc gatctgaccg atcaaataag aggatatttc
    10861 cacttcctgc ggcgcgacct gtacgttgtc ggacgacagc cacgcattaa tccacggaat
    10921 cgggttttga tttgcgcctt caaatccggc cggcaacccc accgcctgca tacgcagatt
    10981 ggtaatatat tcgacgtatt gggataagat ttctttgttc aaaccaatca tcgaaccgtc
    11041 tttaaacaaa tatgccgccc attctttttc ctgttccgcc gcttttttga agagttggaa
    11101 acattcgtcc tgcaactcag cggcaatttc tgccatttca gaatcatcga cacccgaacg
    11161 catcagatta agcatatgct gcgtgccggt caggtgcagg gcttcgtcgc gggcaatcag
    11221 tttgatgatt ttggcgttgc cttccatcaa ctcgcgctcg gcaaaagcaa acgagcaggc
    11281 gaatgaaacg tagaaacgga tggcttccaa cacgttgacg cacatcaggc agagatagag
    11341 ttttttcttc aacccgcgca aagatacggt aacgggtttt ccgccgacat tgtgcatccc
    11401 ttcgcccaac aggttgtaat actgggtgta ttcgattaag tcatcgtaat agcaggcaat
    11461 gtcttcggcg cgggcggtaa tgtattcgtt ttcgacaata tcatcaaaca cgaccgacgg
    11521 atcattcaca atattgcgga tgatgtgggt atagctgcgc gagtgtatgg tttcgctgaa
    11581 gctccacgtt tcaatccacg tttccaactc gggaatcgaa accaaaggca gcaaggcaac
    11641 attcggactg cgcccttgga tggaatcgag cagtgtttgg tatttcagat tgctgatgaa
    11701 aatatgtttt tcgtgttcag gcaggttggc gtagtcgata cggtcgcgcg acacgtcgat
    11761 ttcttccggc cgccaaaaaa acgacaattg tttttcaatc agtttttcaa atacctcgta
    11821 tttctgctgg tcataacggg cgacattcac aggctgacca aaaaacatcg gctcattcag
    11881 cgcgtcgttt ttggttttgg gaaaggtgct gtatgacatg actaggtgtt cgcaggacat
    11941 ggttttctca ggtttattta aatttaggaa tttggatttc agacagcctt atatgtttta
    12001 agtaaggtca tctgaatgta tctgtacatt gaattcttgc cttaaatatc gctgttagtt
    12061 ataatcttcc ccaacaactc ccccaacttt gccaacctac gcggttggag caacaaggtt
    12121 gggggctttc ttttatccct gccacaatct ccagtctcgc ggattatttt ccaaacccat
    12181 atcgcgcaaa cttacagctt cattcgcgac atcgggaaac tcatccagta aagcaagaag
    12241 acggtttccc cattcggttc gcggacaaat aacatccaac atccgtttca tcaggcaaaa
    12301 atacacaaac ggtttattga taggcaatga tttccattct gcatcagaca aacctttcaa
    12361 gctggcttta aaagtaatat ccctattcca taatcgactg tgatgtgccg aaacgttgcg
    12421 gataaagtta aatgcgtgca gatgtgtttg cagatgctta ccgtctttca aacgatacaa
    12481 tttggcaata gagtctttat ctttattcaa catcccttta tacagcatag acatcgtgcc
    12541 gaaatcccat acttcgcagg ctacccagac aggtaagtcg gcataatgtt ccaaatgatg
    12601 tttaacaaaa ccggttttct tttcgcgctc gaccagcctg ttatacctat ccagccattc
    12661 cgcatggtcg aaacctgtgg cagaatattt gctatctttg taagccaatg ggtcatgccg
    12721 tcctaatgta tgggcaatat ttacccgaag cgctacttca attctttcca gtgcatctaa
    12781 tgccaattgc cgcaatttct tatcgaacat ataaaggctt ttgacatctt caaaacgagt
    12841 atctgtaata aactcatcca acctgctctt agaactttca ggagcaaatt gtcggaaaga
    12901 gtaaaaataa ccgcttaatc ggtaataacc gatggtgcgt aaatatccta atgccttttt
    12961 ctcatcatca atctgcaatc ctctacttct tagaatctcc aactgctcat cgtaagattt
    13021 ccaaagcttg aagcctttta tctgcttgct ttgcacagcc tgtttcactt tcaaatagtt
    13081 aaatcttaca agccccgccc gcgcagccgt catcctgaat atcggtctgc gtatcgtccg
    13141 cgccgtcgcg ggtgttatgg tagtacaggg ttttgacgcc gtatttgtag gcggtcagca
    13201 ggtctttgag catttgtttc atagaaactt tgccgccttc gaatttgccc gggtcgtagg
    13261 cggtattggc ggaaatcgat tgatcgacga atttttgcat cacgccgaca agtttcaggt
    13321 agccttcgtt gccgggaagc tgccacaggg tttcataggc atttttcagg gtttcaaact
    13381 ccggcacgac ttgtttcaaa atgccgtctt tcgatgcttt gaccgttacc aatccgcgcg
    13441 gcggctcgat accgttggtg gcgttggcga tttgcgaact ggtttcagac ggcatgagtg
    13501 cggttagagt agagttgcgc aggccgtatt tgacgatttc ggcacgcagg ctttcccagt
    13561 cgtaatgcaa aggctcgccg cagacggcat ccaaatcttt tttgtaggtg tcgatgggca
    13621 gtttgccttg cgaataaacg gtttggttaa agagcgtaca cgcaccgtat tctttggcaa
    13681 ggtttgccga tgctttgagc agataatact gtatggcttc aaaggtacgg tgggtcagac
    13741 cgagcgcgga gccgtcgctg tagcggacac cgtttttcgc cagataataa gcatagttga
    13801 tgacgccgat gccgagcgaa cggcgaccca tagtagaggt acgcgcggct tctaccggat
    13861 atccctgata atctaaaagt gcatcgagcg cgcgaacggt caagtcggca agcccttcca
    13921 attcgtccaa gctgtttaat gcgcccaagt taaaggcaga cagtgtacac aaggcgattt
    13981 cgccgttcgg atcgttgata ttgtccagcg gtttggtcgg cagggcgatt tccatacaca
    14041 agttggactg atgaacaggc gcgacgcgcg gatcgaacgg gctgtgcgta ttgcagtgat
    14101 cgacgttttg aatgtagatg cgcccggttc cggcacgctc ctgcatcagc gtggaaaaca
    14161 ggtcggcagc cggaatgatg cgcttgcgga tatcaggggc ttgctcgtat ttcgtataga
    14221 gccgctcaaa ttcgtcttgg tcggcaaaaa acgcttcgta caatcccgga acctcgttgg
    14281 gcgaaaacag cgtaatgttg ccgcccttaa tcaggcgggt gtacagcagg cggttgattt
    14341 gcacgccgta atcaagctga cggatacggt tgtcttccac accgcggttg tttttcaaca
    14401 ccagcaggct ttcggcttcg atatgccaca aagggtagaa caaggttgcc gcgccgccgc
    14461 gcacgccacc ttgcgaacag gatttgaccg ccgcctgaaa cattttaaag aagggaatgc
    14521 agccggtatg ccgcgcttcg ccgccccgga tttcgctgtc caaaccgcgg atacgtccgg
    14581 cattgatgcc gatgcccgca cgctgggaaa cgtatttcac aatcgcgctg gtagtggcat
    14641 tgatggaatc caaactatcg tcgcattcaa tcagcacaca gcttgagaac tggcgcgtag
    14701 gcgtacgcac gccgctcata atcggggtcg gcagcgatac tttaaatgtg gaaacggcat
    14761 cgtaaaaccg tttgacgtaa tcgaggcgtg tttctttggg atatttgctg aaaagacaca
    14821 tcgccaccaa aacatataag aactgcggtg tttcataaat ctggcgggta acgcggttct
    14881 gtaccagata tttgccttcg agctgtttga cagcggcata ggaaaaggac atatcgcgtt
    14941 cgtggtcgat ataggcgttc agctcgtcaa attcttcgcg gctgtaatcc tcaaggatat
    15001 gcctgtcgta ttttccagca tcggtaagtt ttttaacgtg gtcgtaaagg tgcggcggct
    15061 cgtactcgcc gtaggctatt ttacgaagat ggaaaatggc caaccgcgcg gcaaggtatt
    15121 ggtagtccgg ggtatcttcc gaaattaaat cggcagcggc tttgatgatg gtttcgtgga
    15181 tgtcgtcggt gcggatgccg ttgtagaact ggatgtgcga tttcaactcg acctgcgaca
    15241 cggaaacatt ttccaatccg tccgccgccc aagtgacgac acggtgaatc ttatccaaat
    15301 caatggcttc taatcttccg tctcgtttgg ttactttcaa atcagtcggt gtattcatcg
    15361 cttcctcttc cactcttgat attcaagaca cggtcttttc aaataaatta agacagacaa
    15421 tatagtggat ttttggcatt ttctccagtc ttgacaacgg ttgcattttt cagtttcagg
    15481 caatgcgcga taaaacccgc ctgtcgtatt tttcctataa tatttgtttt atctgataat
    15541 tctttaccga taaaaaacgg gtaaattttt tgccttttga ccggctccgg ctacaaggcg
    15601 gtgaaataag gattttccga cgaaaaagga aagcttccgg ttttctgccc ctgcgaattg
    15661 ttaaattttg caaagtatga ttttgccgcg ccgccgccga caaattccat tttcttaccg
    15721 attgaaattt attattgaga ttaatgtgtt atttgaatcc gcatatcaaa cggcaagttt
    15781 tgtcggctga atgagtctga aactgccgac aatttgcccg ttccatcctt ccatcctata
    15841 ctggaaggaa tgacaaactg aaaggatcgt catgtctgtc aaaaagatgc ttgccatact
    15901 gttgtctgca atattgggac tggtatcgac aactgccgct gccggtacgt cagaacccgc
    15961 ccaccgcgat accaaacata tccgcaaggc aaacaagcag atgctgcacc ccgaatgcag
    16021 gaaatatttg gaacgccgtg ccgcgtggta ccgatcgcaa ggcaacgtgc aggaattgcg
    16081 cgaaaacaaa aaggcgcgca aagcattccg ctccctgcct tatgcggaac agaaaatcca
    16141 atgccgggcg gcttatgagg ctttcgatga tttcgacggc ggcagtttcc gccgttaatc
    16201 ccatataaaa tatgccgtct gaacacaggt tcagacggca ttttccatag atacaacaaa
    16261 ccatccttac gccatttcgt ttgaggtact cccgttgctg cagccttaat atagtggatt
    16321 aacaaaaatc aggacaaggc gacgaagccg cagacagtac aga
//
LOCUS       DAXHSJ010000028        16259 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000028 DAXHSJ010000000
VERSION     DAXHSJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.028"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(49..1242)
                     /gene="odhB"
                     /locus_tag="ACFM8U_000680"
     CDS             complement(49..1242)
                     /gene="odhB"
                     /locus_tag="ACFM8U_000680"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC6333959.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPVAAPAEAAPAAAPAA
                     APDAAQNNAAMPAATKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVA
                     LPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEK
                     EHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPIL
                     RDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQ
                     SAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPAR
                     LLLDL"
     gene            complement(1342..4170)
                     /locus_tag="ACFM8U_000681"
     CDS             complement(1342..4170)
                     /locus_tag="ACFM8U_000681"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC6333960.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4375..5658)
                     /gene="gltA"
                     /locus_tag="ACFM8U_000682"
     CDS             complement(4375..5658)
                     /gene="gltA"
                     /locus_tag="ACFM8U_000682"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC6333961.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(5770..6018)
                     /locus_tag="ACFM8U_000683"
     CDS             complement(5770..6018)
                     /locus_tag="ACFM8U_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC6333962.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6022..6729)
                     /locus_tag="ACFM8U_000684"
     CDS             complement(6022..6729)
                     /locus_tag="ACFM8U_000684"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC6333963.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6849..8612)
                     /gene="sdhA"
                     /locus_tag="ACFM8U_000685"
     CDS             complement(6849..8612)
                     /gene="sdhA"
                     /locus_tag="ACFM8U_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC6333964.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYQGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDINTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8615..8956)
                     /gene="sdhD"
                     /locus_tag="ACFM8U_000686"
     CDS             complement(8615..8956)
                     /gene="sdhD"
                     /locus_tag="ACFM8U_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC6333965.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8950..9327)
                     /gene="sdhC"
                     /locus_tag="ACFM8U_000687"
     CDS             complement(8950..9327)
                     /gene="sdhC"
                     /locus_tag="ACFM8U_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC6333966.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKFVFTAALVLTVILGALLW"
     gene            complement(9606..11009)
                     /locus_tag="ACFM8U_000688"
     CDS             complement(9606..11009)
                     /locus_tag="ACFM8U_000688"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6333967.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHIVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11258..11995)
                     /locus_tag="ACFM8U_000689"
     CDS             complement(11258..11995)
                     /locus_tag="ACFM8U_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC6333968.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12272..14548)
                     /gene="metE"
                     /locus_tag="ACFM8U_000690"
     CDS             complement(12272..14548)
                     /gene="metE"
                     /locus_tag="ACFM8U_000690"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC6333969.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAAGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTIVGPL
                     TFLWVGKEKGVVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLKTVESLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAAGSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSVADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(14686..15564)
                     /gene="metF"
                     /locus_tag="ACFM8U_000691"
     CDS             complement(14686..15564)
                     /gene="metF"
                     /locus_tag="ACFM8U_000691"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC6333970.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15765..16040
                     /locus_tag="ACFM8U_000692"
     CDS             15765..16040
                     /locus_tag="ACFM8U_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC6333971.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16040..16165
                     /gene="ykgO"
                     /locus_tag="ACFM8U_000693"
     CDS             16040..16165
                     /gene="ykgO"
                     /locus_tag="ACFM8U_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC6333972.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRKGKVYVICKSNPRFKARQR"
BASE COUNT         4022 a         4092 c         4019 g         4126 t
ORIGIN      
        1 aaattcagac ggccttttca ttaacaaata aaaggccgtc tgaaacgatt acagatccaa
       61 caacaggcgg gctgggtctt ccaacgcgtc tttaatggct accaaggtca atacagcttc
      121 gcggccgtca atgatacggt ggtcgtaaga cagagccaga tacatcattg gacggacaac
      181 aacttggccg ttttcaacca cagcgcgctc tttagtggcg tgcataccca aaatcgcaga
      241 ttgaggcggg ttgatgatcg gggtagacat catagaaccg aaagtaccgc cgttggtaat
      301 actgaatgta ccgccggtca gatcttcgat agcgattttg ccgtctttgg cttttttcgc
      361 gtaatcaaca attgcttgct cgatgtcggc aatgctcatt tggtcggcat cacgcaggat
      421 tggcacaacc aaaccgcgtg ggctgccaat cgcaataccg atgtcgaagt agccgtggta
      481 cacgatgtct ttgccgtcaa cagaagcatt cacaaccggg tattttttca gggcggcaac
      541 agcggcttta acgaagaagg acataaagcc caatttcacg ccgtgttctt tctcgaattt
      601 ttctttgtac ttcgcacgca agtccatgat tggtttcatg ttgacttcgt tgaatgtagt
      661 cagaatggcg ttttcttgtt gagaagccaa gaggcgttct gcaacacggg cacgcaggcg
      721 gctcattggt acgcgttctt caggacgtgc gccggcagga agtgcaacgg caggggctgc
      781 ggctgcggca ggtttggcag cggcattttg tacgtcttct ttcaatacgc gaccgtcacg
      841 gccggaacct tgcaatgcgt tcacgtcaac accggtctcg gcagccagtt tggtggcagc
      901 aggcatagcg gcgttgtttt gtgcagcatc aggagcggca gcaggagcgg cagccggggc
      961 ggcttcggca ggagcggcta cgggtgcttc ggcagcggca gtagcagctg tatcgatgcg
     1021 tgccaaaact tggtcggcaa caacggtttc accgtcttgc gctacgattt caaccaatac
     1081 gccggcttgt ggagaaggta cttccaaaac cactttgtcc gtttcgatat cgatcaggat
     1141 ttcgtcacgg gcaactgctt cgccaacttt tttcttccat tccaagagcg tgccttcaga
     1201 tacgctttca gacaacatag gtacttttac atcaataatc attttgtgtc tccaatggcc
     1261 ttttcagacg gcctgttgtg tgtttttaat tctgcatatg ggcattccat ctgagcagac
     1321 tcagacggaa tgctacttgg tttacaatgc caaagcgtct tcaaccaatt gtttcaattg
     1381 agcaatgtgt ttgcttgagt agcccactgc aggcgatgcg ctgcttggac gaccggcata
     1441 agacagtttt tgctcttcgc taataacatc ttcgatgcgg tggcggattt ggtagaacgc
     1501 gccttggttt ttcggctctt cttgcgccca aaccacagat tttgcgttcg gatatttcgc
     1561 cagttcggct ttaacctcat cgtatgggaa cggatacagc tgctcaacgc ggacaatcgc
     1621 aacatcatct tccagtttac gttcggctcg accggcttcc aagtcatagt aaacctgacc
     1681 ggcacacaat accacgcgtt tcacgctgtc gttgcttgcg cgttcggcgg tatcgccgat
     1741 aaccggacgg aaagtcgaac cttcggtgaa gttttccagc gggctcattg cccctttgaa
     1801 tcgcaacagg cgtttggaca tgaaaattac cagcggtttg cggtatgaac ctaagacttg
     1861 acgttgcaag aggtggaaca tttgcgacgc ttcagatggc atgatgactt gcatattgtt
     1921 ctcagaacac agttgcaacc agcgttctac gcgtgcagaa gagtgctcag ggccttgacc
     1981 gtcgtaaccg tgcggcagga tggtggtcag accgcacaag cgaccccatt tggtttcgcc
     2041 tgaagacagg aattggtcaa tcgtcacttg cgcgccgttg gcgaagtcac cgaattgagc
     2101 ttcccaaatg gtgagcttgt caggagcgga gcaggcaaag ccgtactcga acgccatcac
     2161 ggcctcttcg ttcaaaatgg agtcgataac caggaactcg cccattcctt cgcccatatg
     2221 tcgcagagga acataagtac cgtcgtccca tttttcgcgt ttttgatcgt gcaatactgc
     2281 gtggcggtgc gagaacgtac cgcggccgga gtcctcaccg gagatacgca cgccatgacc
     2341 tttggttaca aggcttgcgt atgccaaggt ttcggccata ccccagtcga tggcttgttt
     2401 gccggatgcc atggctttac gcgcttcaat cacacgtttt gcagtcggat gcagggcaaa
     2461 gccttccggt acggcggtaa atttctcggt aagacgctca atatccgcgg caggcaaacc
     2521 ggtttcgatg tgttcgcgcc aatctttacc ttggtatttg ctccaatcga tttgtgtgcg
     2581 ttggaaattg ctcaacgttg tttgctcaac gtgttcgcct ttgtccaaag catcgcggta
     2641 ggcttggatg taaccgtctg cctcggcttg agttaccaca ccttcggcaa tcagttgctc
     2701 ggtgtacaaa gcacgcgcac caggatgttg cgatactttt ttgtacatca tcggttgggt
     2761 caaggtcgga tcatcgccct cgttgtgacc ccatttacgg tagcagacaa cgtcaatcac
     2821 gatgtcttta tggaattttt tgcggtaatc caaagcggct tggatagcaa agcaaacgcg
     2881 ttcgggatca tcgccgttca catggataac cggggcggaa accatttttg cgatatcggt
     2941 acagtgtacg gttgaacggg tatcgcggat atcggaagtg gtaaagccga tttggttgtt
     3001 gatgacgata tgaaccgtac cgccggtggt ataaccgcgc gttttagaca ggttgaatgt
     3061 cgcttggttg actcccagac cgataaatgc ggagtcgccg tgaatcaata ccggcaagac
     3121 tttgtcgcgg ccgttttcgc ccaaacgttt ttgtttagcg cgcgcagaac cttccaccac
     3181 cgggttgacg atttccaagt gtgacgggtt gaacgccaaa gaaacatgca tcgggccgtg
     3241 cggggtagca atatcggagc tgaagcccat atggtatttc acgtcgccgc tgggcagttt
     3301 gatttcggca cgaccttcaa attcggcaaa caaatcgccg ggttttttgc ccaaaatgtt
     3361 caccaaaaca ttcagacggc cacggtgcgc cataccgatg atgacttctt ccacgccgtc
     3421 tttaccggcg ttttgaatca ggtagttcaa accggcaatc gcgctttcgc cgccttcgac
     3481 accgaaacgt ttctgaccga catatttggt atgcagataa cgttccaaag tctcggcagc
     3541 agtcatctct ttcaagatac ggcgtttttg atcggcattg taatgcggtg tggacaatac
     3601 gctttcaaaa taattgcgta cccagcggcg ctcttcggta ttgggaatat agatatattc
     3661 caatgcgatg tggccgcagt aggtttgttt gaggttgctg atgatttggg acaaaggcag
     3721 tttgccgcga ttggcaaaat cgccctcgcc catattgaat tgaagcgcca tatcggcatc
     3781 tgacagaccg tggaatttcg gatcgagggc ttcaatatcg cgcgggggga tacgtttgag
     3841 cggatcaagt tgggctgcac ccacgccttg gatacgatag gcggaaatca gccgtaaaac
     3901 gctgacttgc tttttcagca ttgcctcatc cgcaccgccc gcaacggcag atgcaatttt
     3961 ctttttcgcc aaagtaacaa atgattcgcg aatcggtgtg tgtgcgacat cgacagcaac
     4021 cgtccccggc tgtttgctca aatcggtgaa atactgcttc catttttcat caaccgcatc
     4081 ggggttttcc aaaaaagcct cgtacaattc ctcaatgtag ggtgcgttcg aaccgaacag
     4141 gtaagagaaa ttgagttttt cgtccatcat ggcgtgcgct cttttcttta aaatataaac
     4201 aaagggtaga agtccgcgtc ctgacggcta aactgatgcc gtctgaaaag acattttatt
     4261 ctaccctatt ttaaaccgga ttgcttgtct tgatttcatc cgactacaat cagacggatt
     4321 cgggaaacaa tccggttctc tgatattgcc tgtttgacaa tatattaatg tatgttacct
     4381 ctcgcctggc ggcacataat cgcggcgttc cgaaccggta taaagctggc gcgggcggcc
     4441 gattttcagc gaaggatcgc taatcatctc gtgccagtgc gaaatccagc ccacgctgcg
     4501 cgacagggcg aagatgacgg taaacatttc ggtcgggatg cccagcgcgg acaggacgat
     4561 gccggaatag aaatcgacgt ttggatacag tttgcgttcg ataaagaacg ggtctttcag
     4621 cgcaatctgt tccaattcca tcgccagttt gaatttcgga ctgtcttcca agcccaattc
     4681 cttcaaaact tcatagcagg tttcgcgcat aatgctggca cgcggatcca tattgcggta
     4741 cacgcggtga ccgaagccca tcagacggta tttgcgttgt ttcacacctt ccatgtatgc
     4801 ggcaacatta gacacatcgc cgatttcgtc caacattttc aacacggctt cgttcgcacc
     4861 gccgtgcgaa gcaccccaca ggcaggcgat accggcagca atacaggcaa acgggttcgc
     4921 acccgaagac cctgccagac ggacggttga agttgaggcg ttttgctcgt ggtcggcatg
     4981 caaaataaag atgcggtcga gcgcgcgtgc caaaacggga ttgggtttgt agtcttcaca
     5041 cggcgtggcg aacatcatat gaaggaagtt ttcggaataa gaaagattat tcttcggata
     5101 attgaacggc agaccgtttg aatagcggta gcacattgcc gcaatggtcg ggattttgga
     5161 aatcaggcgg taaatcgcga ttttgcggtg ttcgggattg ctaatgtcca agctgtcttg
     5221 gtagaacgca gacagcgcac cgaccacgcc gaccatcatc gccatcggat gcgcgtcgcg
     5281 gcggaacccc cggaagaacc aagtcagctg ttcatgcacc atcgtgtggc ggcggacggt
     5341 attgtcaaat tctgcctttt gctcgggagt cggcagttcg ccgtaaatca acaggtagca
     5401 gacttccaaa taatcggact tttcggccag ctgctcgatg gggtatccgc gataataaag
     5461 caagccttga tcgccgtcga tgtaagtaat tttagactca cagcttgcgg ttgaaacaaa
     5521 tccggggtcg aaggaaaaca aacctgtatt ttttgtcagc ccccgaatgt caaccacatc
     5581 gtgcccgatg ctggcttcca ataccggcag ctccaaacct gcctgacccg gtacgttgag
     5641 tttgattgat ttggacatat tattgctcct ttgcaagtta taacccacgt tactgcatcc
     5701 gcagcacgtt ctttgccttc agacggcttt tgcattttga aatctgcctg caggcagatt
     5761 tcagatgcct catgcccgtc tgattttttc aagcatcggg ataaggtgcc ctttgtccgt
     5821 ttccgaatgc ccgttaatca aggcaagcaa ttcttgatct tgaaattcaa ggatttcgga
     5881 aaactcggac agctctttat cgctcaaatg ctcgaattct ttttccataa acctgccgaa
     5941 gattaaatct aattccaaca atccccggcg ggtttgaaaa cggattttcc gtttggcaat
     6001 atcgtcaaaa accatcattt cttaaacggc ccgtttcaac ataatctctt taatcttacc
     6061 gatggctcgg gtcggattca agtgtttagg acatacgtct acgcagttca taatggtgtg
     6121 gcaacggaac aaacggtatg ggtcgttcag attatccaaa cgctcattag tgatggtatc
     6181 acggctgtcc gcaatgaaac ggtaagcatt cagcaaaccg gacggaccaa cgaatttatc
     6241 agggttccac caaaatgacg ggcaggcagt cgaacagcag gcgcacaaaa tacactcgta
     6301 caaaccgtcc aactctttac gctcttcctg agtttgcaga cgctctttgt ccgcatcaat
     6361 cggattatcg ttgacaacat aaggtttgac ggaatggtat tgtttgaaga actgggtcat
     6421 atccacaatc aggtcacgga taacaggcag acctggcaga ggacgaattt taaccggctg
     6481 tttcaagcca cgcagatcgg tcaaacacgc caagccgttt ttgccgttga tgttcatacc
     6541 gtcagaaccg caaatccctt cgcggcagga gcggcggaaa gacaaggtat cgtcttgtgc
     6601 tttcaggcgt accaaagcat ccaaaagttt cacgtcggtc ggttccaatt ccaactcgta
     6661 acgctgcata taaggcttgg catcaacatc cgggttgtaa cggtaaattt caaaactcat
     6721 tttttccatg agaagtgttc cttttctcat caactgccca atgattttgt attataaatt
     6781 caagcagttc aatgtgggta tggttagatt tcagacgacc tttgaggcga agaccgtctg
     6841 aaaacgcatc aataaacgcg cttggccggt ttgatgtatt ccacgctcaa aggcttggtg
     6901 tgcaccggtt tgtaggacaa ggtattgata tctgaatggt acagcgtatg tttcatccag
     6961 ttttcatcat cgcgctcagg atggtcgtct gaagcgtgcg caccgcgtga ttctttacgt
     7021 gcttcggcag acaccaaagt cgctttcgcc acttcaatca ggttatccaa ttccaaagcc
     7081 tcgatacgcg cggtattcca cactttgctc ttgtctttga tttcggtacg tttcacacgc
     7141 tcggcaatcg ccatgacttc tcgaacgcct ttgctcagaa tctcatcagt acggaacacg
     7201 ccggcgtgca gttgtacaga gcgttgcagt tcgcggcgca atgcatcaac gttttcgcca
     7261 tcagtttgat tgtccaaacg ctcgatacgt tggcgggtca gctcgcccgc atccgcaggc
     7321 aaaggtttcc agtcgctttg ctctttgatg aatttaatca tgctgtcgcc ggcagcttta
     7381 ccgaacacca ccaagtccag cagagagttc gtacccaaac ggttcgcacc gtgtacggaa
     7441 gcacaggcgc actcacctgc ggcatacagg ccttttacag gcacttcgta ttcgtctcct
     7501 tgcggcacaa cgacttcgcc ttggtaattg gtcggaatgc cgcccatcat atagtgggta
     7561 gtcggcacaa caggaatcgg gtctttaatc ggatcgatac cggcgaactg aatggaaatc
     7621 tcgcggatgc ccggcagttt ttccataatt ttttctgcgc cgatatggtc gattttcagt
     7681 aagacatggt ctttgttttt accgcagccg cgaccttcgt agatttccat cgccatcgcg
     7741 cgggaaacaa cgtcgcgaga agccaagtct tttacggtcg gcgcatagcg ctccataaag
     7801 cgttcgccgt cggcgttcaa caaaataccg ccttcaccgc gtacaccttc ggtaatcaac
     7861 acgcctgcac ctgccacacc tgtcgggtgg aattgccaga actccatatc ttccaacgga
     7921 atacccgcac gcgcgcaaat gcccaaacca tcgccggtat tcatataggc attggtagaa
     7981 gaagcataaa tacgaccgcc accgccggta gcaaacatca cagctttagc gtggaaaata
     8041 taaacttcgc cggtttccat ttccatggcg gttacgccga cgacatcgcc gttttcatca
     8101 cgaatcaaat cttgtgccgt ccattccaca aagaattgcg tattggcacg gacgttttgt
     8161 tggtacaaag tatgcagcat cgcatgacct gtacggtcgg caaccgcaca ggcgcgttct
     8221 accgcgcgtt taccgtgttc ggcagtatgg ccgccgaaag gacgctgata aattttaccg
     8281 ctttccacac ggtcaaaagg catacccatg tgttccaact caattacggc ttcaggcgcg
     8341 gcgcggcaca taaactcaat cgcatcttgg tcgcccaacc agtcggaacc tttcacggta
     8401 tcgtacatgt gccagtccca acggtcttcc tgcacattac ccagagaggc ggaaataccg
     8461 ccctgcgccg ctacggtatg cgaacgggtc gggaacactt tagacaaaac ggcacaattc
     8521 agaccggatt tggataattg gagggctgcg cgtaaacctg caccaccacc gccgacaatc
     8581 acggcatcaa acttgcgaac aggaaaaccc atacttaccc ccaaattact ttaattgaat
     8641 acaccaagca gccgaccagc cagacgatgg tggcaacctg caaaaacaaa cgcacgccga
     8701 agggtttgat atagtccatc cacaaatcgc ggatacccac ccaagcgtgc aagaatacgg
     8761 cgatgaagct cacttgggta aatactttta cccaagtttg actaaaaaat gcctgccatg
     8821 ccgaatattc tttaggcagg gaaaatagaa ccactaaaag tgcaacggta taaatcaaca
     8881 taataaccgc agtcgcacgt tgcatcgccc aatcgcgcaa accgtaatgg gcaccggtca
     8941 atttacgttc taccataaca acgctcccaa aatgacagtc agcaccaaag ctgcggtaaa
     9001 gacaaattta gcggtgttcc gtgcagtatt cagctcaaga cctttgtgcg catccaaaaa
     9061 taaaaagcgg ataccggcaa gggaatggtg cagataagcc cacaatacgc cgattaagac
     9121 cagcttgacc aaaggatggg aaacaatggc acggtaagtt tcaaatgcag attcttgact
     9181 tagggtgccg gacaggaaat acagcaggaa aggcagcata ataaacagcc cgaccccgct
     9241 gatgcggtga aggatggaaa ctatcccggg tatcggcaga cggatgttcg gcaaatccag
     9301 ataaacagga cgcggtttgg cagacatagt caattcctct gtagttatat atcgactcaa
     9361 aaaccgtaaa cccattgcta cactgggtta cataaacgtt aatttacctg ttttatccca
     9421 ttttgaacag tgtattttaa aaacaaactg atagattttg acaaacacgc gtggaaacag
     9481 cagattaaaa gaaagatttt tagttttgcc gcccccttcc cgacacggta tcagcatccg
     9541 accccatccc caaaaacaaa atgccgtctg aaaaaatttc agacggcatt gttttgccat
     9601 atcggtcagg ctttcaattt cgcatcggca tcgcgcaacg cggtacgcag accttcctcg
     9661 ataacgggat ggtagaacgg catatccagc atttgcggaa cggtcatctt catttgatgc
     9721 gcccatgcca acaagtgcgc caaatgttcg gcggcagggc ctacgatttc cgcgccgata
     9781 aagcggccgg tggctttttc ggcatacagg cgcatatggc ctttgttcac cagcatcacg
     9841 cggctgcggc cttggttttt gaacgatact tcgccgatga caaattcgtc ggcttggtat
     9901 tgcgcggcaa cctgcgcgta tttcagaccg acaaagccga tttgcggact ggtaaacacc
     9961 acgccgatgg tgctgcgccg caaaccgccg ccgatattcg gatagcgacc cgcattgtcg
    10021 ccggcaatct taccttggtc ggcggcttcg tgcagcaagg gtagttggtt agacgcgtca
    10081 cccgcaataa agatatgcgg aatgctggtc tgcatagtca acgggtcggc aacgggtacg
    10141 ccgcgcgcat ctttttcgat attgatgttt tccaaaccga tattgtccac gttcggacgg
    10201 cggcctacgg ctgccagcat atattcggca acaaatacgc ctttttcgcc atcctgctcc
    10261 caatggactt ctacattgcc atctgcatcg agtttgacct cggttttggc atccagatgc
    10321 agtttcaatt cttcaccgaa cacggctttc gcctcgtctg aaacgacggg gtcggaaatg
    10381 ccgccgatga tgccacccaa accgaaaatt tcaactttca cgcccaaacg gtgcaatgcc
    10441 tgacccagtt ccaaaccgat aacacccggt ccgaacacgg caacgcgctt aggcagcgta
    10501 tcccatgaga aaacgtcatc gttgataatc aaacgattgc ccaaagactg ccattgcggc
    10561 aggatgacgg gacgcgaacc ggtagcaatc acgaaacttt ttgccgtaat ttgagtatgc
    10621 tcgtcgattt ggacgatatg ctcgtcgata aatttagccg aacccataat gcgcttgtcg
    10681 gcaggccact cttccacatc ggcaacgaca aagccgacaa aacggtcacg ctcggattta
    10741 acgcgctgca tgacctcttc accgttgacg acgatgctgt ctttgtccaa atgcacgccg
    10801 aacgggtcgg tatgcaatgc gtgatgacgc gcctctgcgg cggcaatcaa gagtttggaa
    10861 ggcatacagc ccacgcgcgc gcaggttgtg ccgaacacat tgttttcaat caggtaaaca
    10921 ttatccgaat gtaaacgggc attgcgaaac gcacccatac cggcagtacc gccgccgatt
    10981 acgacgacat ccgcttgaat ttttttcata atctttgtcc ttccgttttc gtctgaagca
    11041 ttgccgacac agaacatatc ggtttcagac agcatttaaa tattttccca atcaaaaaca
    11101 tcattcagat aaataaaaac aaaatttcga ttgagtgcaa caatatccaa attctcttat
    11161 aaatatagag ttattcggag cggcagtgta gaaccgcccg aataactcta tccggtatat
    11221 cagacagttt aatcaaaccg ccttaagcaa acagctatca gtttttagcc aagtaagctt
    11281 ccaaatcttc gctgccgccg atgtatttgc cgccgatgaa gacttgaggg gcagtcatct
    11341 tgccggtaat ggcgcgaacg gaagtaacgg ttgcatcttt gcccaatacg atttcttcgt
    11401 aagacaaacc tttgtcttgc aaagcttgtt tggctttagc gcagaattgg caacctggtt
    11461 tagtgaaaat tgccacagac tcttgagcct tccaatcggg agcaacgaat tgcagcatag
    11521 tatctgcatc ggatactttg aacggatcgc ccggttcttc aggttcgatg aacatttttt
    11581 caaccacgcc gtcgttaacc agcatggagt aacgccaaga gcgtttaccg aagcccaagt
    11641 cttctttacc gaccagcata cccatacctt cggtaaattc gccgttgccg tcaggaatca
    11701 tgtagatgtt gtcggattct tcttcggcag cccaagcgtt cattacgaac gtatcgttta
    11761 cagatacgca gtagattgcg tcaacgccgt tttctttgaa cgcgccgaac aattcgttgt
    11821 aacgcggcag gtgtgaagaa gaacaagtcg gggtaaatgc accgggcagg gagaatacga
    11881 ctactttttt gcctttgaac aaatcatcgg tagacacatc tttccaagtg tcgccgacgc
    11941 gggtgcggaa tactacggaa ggtacttttt gaccggtacg atcttgcaaa gccattttga
    12001 gctccttaat agataggttt aagaaatgat tgtgaatcgc tgggcattct accccataca
    12061 aaatgatttg tataatttat agttcaaata atattaatta ttcaaaacta ttaatcacca
    12121 acctcgacca acttcttaca gtatataaag ccatgcctaa aaaaaacaag tcttatttga
    12181 gaaaaattct tagaaacccc cctacgctca tttgaaaaca gaaatgccgt ctgaaaagta
    12241 ttcagacggc atccaatcac gttatccctg cttatttagc cagttcggca cgcagtttgc
    12301 gggttacgtt catcatcact tggagttgtt ccagagtttc tttccagcca cgtgttttca
    12361 ggccacagtc tgggttaacc cacagacgtt caaccggtac aacctcgatg gctttgcgca
    12421 acaggtgctc aacttcagct tcagtcggta cgcgcgggct gtggatgtcg taaacgcctg
    12481 ggccgatgtc gttcgggtat ttgaattcgc cgaacgcggt caagagttcc atatcggaac
    12541 gtgaagtttc gatggtgatg acatcagcat ccatagcggc gatggccggc aagatgtcgt
    12601 tgaactcgga gtaacacata tgggtgtgga tttgggtgct gtcttcgcaa ccggcagagg
    12661 acaggcggaa ggattcgccc gcccagttca ggtaggcatc ccaatcggcg cgtttcaaag
    12721 gcagaccttc gcggatggca ggttcgtcaa tttggatgac tttgatgccg gctttttcca
    12781 gatccaatac ttcgtcgttc agggccagtg cgatttgttt gcatacggta gcgcgaggaa
    12841 tatcgttgcg gacgaaagac cattgcagaa tggtaacagg accggtcaac atgcctttca
    12901 tcgggcgttt ggtcaggctt tgtgcgtaag tagaccaagc cacagtcatg gcttcaggac
    12961 ggcttacgtc accgaagata atcggtggtt taacacaacg tgagccgtag ctttgtaccc
    13021 agccgtattg ggtgaatgca aaaccgctca acaattcgcc gaagtattcg accatgtcgt
    13081 tacgctcggc ttcgccgtgt accagtacgt ccaaatccag tttttcttgt tcttcaacca
    13141 ccaaggcgat ttcttttttc atcgcagctt cgtaatcggc gacagacagt tcgccttttt
    13201 tgaaagctgc acgtgcttgg cggatttcgg tagtttgcgg gaaagaaccg atgttggtcg
    13261 tcggcagcag aggcaggttc aaccatgctt gttgcgcttt gatacggtcg gcaaatggag
    13321 atttgcgttg gtctgcgttg gcaggcaaat cagccaggcg tttggcaacg tctgcacggt
    13381 ggatttcgct gctgttggcg cgtgagccgg cggcagcttg gctggcggcg agttcttcgg
    13441 caacagaatc acggccttcg ttcaatgcag ctttcagaac gcgcaattct tgggtttttt
    13501 gcagggtgaa tgccaaccaa gagtacaggt caggtttgtt ggctttcagt ttttcttcaa
    13561 ctgacaagtc aaatggagtg tgcagcagag agcaagagct ggagatccac aaacggtcgc
    13621 ccagtttggc ttgcagagac tcaacagttt tcaaaacttt gttcaggttg gcgcgccaaa
    13681 tattgcggcc gtcaatcacg ccggcggaca ggactttgtc gtagtcggcg aacgcgtcca
    13741 gttgctcggg agcgcgtacc aagtcgatgt gcagaccgtc aacaggcagg gctttcagca
    13801 atgcggcgtg ttcggcaaca gaaccgaagt aagtgctcaa caggattttg gcacttactt
    13861 tgttcaaagt agcgtaaacg tctttgtagg cttccaccca ttctttaggc aaatcgacag
    13921 ccaaagcagg ctcgtcgatt tgaatccact cggcaccggc ttcaaccaaa gcagtcagga
    13981 tttcaacgta aacaggcaac agtttaggca acaggctcag acggtcgaat tcgacgacgc
    14041 ctttttcttt acccacccac aggaaagtca acggaccgac gatggtcggt ttggctttca
    14101 gaccgagggc ttgggcttct tgcaattgtt ggacgtagtg tttggcattg gctttgaatt
    14161 cggtatcggc gtggaattca ggcaccaagt agtggtagtt ggtgtcgaac catttggtca
    14221 tttcgatagc gaattggtct ttgttaccgc gcgccagttg gaagaattgt tccaaagaca
    14281 ggttttggct gtcgaagccg aagcgggcgg gaatcgcgcc ggtggcgact tgcaggtcga
    14341 ggatgtggtc gtagaaagtg aaatcgcctg cggcaacgta atcggcgttg gcagcagctt
    14401 ggtgtttcca gtttttctcg cgcaagtctt tagcaacagc cagcaattct tgctcgctga
    14461 tttctttgcg ccagtatttt tcttgtgcga atttcaattc gcggaaggcg ccgacacgcg
    14521 ggaagcctga gaaatgtaat gttgtcatgt tgaactctcc agttggattt tgtaaggacc
    14581 ggttcgatcc gaatcggccg gtattgtaaa tcagaatggc tcggatgggg cggtgttttg
    14641 ctttttagac accttagatg ccgtctgaaa ctgtttgata cggctttaag ggcgcacgcc
    14701 taaaatatgg caaatggcgt aagtcagctc gctgcggtta agcgtataga agtggaaatc
    14761 tttcacgcct tcgcgggaca ggactttgac catatcgatg gcgatacttg ccgccaccag
    14821 attgcgcgtg ccttggtcgt cgtccaaacc ttcatacatt tgcgacagcc agcttgggat
    14881 tttgacgttg gttacctgcg ccattttgcc gagctgcttg aagttggtaa cgggcaggat
    14941 gccggggacg atttccacat cgatgcccaa catcacgcag cggtcgcgga agcgcaggta
    15001 gcgttccaca tcgaagaaga attgggtgat gacgtggttc gcgcccgcat cgattttgcg
    15061 tttcaaatta atcaggtcgg cttgtgcgga tttcgcttcg ggatgcactt cgggatatgc
    15121 cgctacagaa atgtcgaagt cggcgacgga gcgcaacagt ttgaccaagt cttcggcgta
    15181 aaacggtttt ttctcataac cggccggctc gtctccgcgc agggcgacaa tgcggcggat
    15241 gccgctgtcc caataatctt tggcaatttg gcgcaattcg tcgggagaag cgtcgatacc
    15301 ggtcaggtga ggcgcggctt ccaagccggt ttcctgtttg atgcgtttga cgatgccgtg
    15361 cgtgcggtcg cgttcgccgg aatttgcgcc gtaagttacg gatacgaact tgggatgcag
    15421 ggtttgcaga cggtggatgg agtcccacag catcgtttcc atttgttcgt ttttcggcgg
    15481 gaaaaattca aacgaaacgt taatgtcgcc tttcaaatca gaaagactgt tatttaacgc
    15541 attgatttct tttgcataat tcatggctgc gtacctttta ttatctttta tatgatattt
    15601 tcaggcaaca taataaagtt acgccaaaca tgagtcaatt tcaaaatttt cagatttatc
    15661 atgaaaattt ctaatatatt acaaaaatag ttaaatttcc ttgtacaagc cttctaattt
    15721 atgataaagt ttgttatgtt ataacaattc aactaaggcg atatatgaaa ccgaatattc
    15781 atcctgacaa ttatcgaacc gtcctttttt tcgacagcag cgccaatgaa ggctggttaa
    15841 tccgctcctg tgccgaaaca cacggaaaaa cgatggtttg gacagacggc aaagaatatc
    15901 cgctgttttc gctggatact tcttccgcat cccatcccgt ctatactggc aaacagcgca
    15961 acgtcaacac cgaaggccgt gccagcaaat tcaaccaacg tttccaatcc gtcatgtcat
    16021 cgtttagaaa ggacaaataa tgcaggttct gtcttcactc aaaaccgcca aacaacgcca
    16081 ccgcgactgt cagattgtca gacgtaaagg caaagtttac gtcatctgca aaagcaatcc
    16141 gcgttttaaa gcgcgccaac gctgaccccc tgcccttcct gcttcggttg tatagtggat
    16201 taacaaaaat caggacaagg cgacgaagcc gcagacagta caaatagtac ggaaccgat
//
LOCUS       DAXHSJ010000029        16182 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000029 DAXHSJ010000000
VERSION     DAXHSJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.029"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(261..1340)
                     /gene="apbC"
                     /locus_tag="ACFM8U_000694"
     CDS             complement(261..1340)
                     /gene="apbC"
                     /locus_tag="ACFM8U_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC6333973.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPETGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIAKIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
     gene            1551..1626
                     /locus_tag="ACFM8U_000695"
     tRNA            1551..1626
                     /locus_tag="ACFM8U_000695"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1584..1586,aa:Lys,seq:ttt)
     gene            1705..2214
                     /locus_tag="ACFM8U_000696"
     CDS             1705..2214
                     /locus_tag="ACFM8U_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC6333974.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            2557..2997
                     /gene="nadR"
                     /locus_tag="ACFM8U_000697"
     CDS             2557..2997
                     /gene="nadR"
                     /locus_tag="ACFM8U_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC6333975.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3112..3612
                     /gene="hpaC"
                     /locus_tag="ACFM8U_000698"
     CDS             3112..3612
                     /gene="hpaC"
                     /locus_tag="ACFM8U_000698"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC6333976.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRKARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            3628..4323
                     /gene="murU"
                     /locus_tag="ACFM8U_000699"
     CDS             3628..4323
                     /gene="murU"
                     /locus_tag="ACFM8U_000699"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC6333977.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4568..4999)
                     /locus_tag="ACFM8U_000700"
     CDS             complement(4568..4999)
                     /locus_tag="ACFM8U_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6333978.1"
                     /translation="MGSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            5174..6850
                     /locus_tag="ACFM8U_000701"
     CDS             5174..6850
                     /locus_tag="ACFM8U_000701"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC6333979.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLADLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGVGGADLARKVVNAIENQPNNFNFSYDVELSIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPVCMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            6965..8056
                     /gene="ychF"
                     /locus_tag="ACFM8U_000702"
     CDS             6965..8056
                     /gene="ychF"
                     /locus_tag="ACFM8U_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC6333980.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVELCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LERLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            8326..8523
                     /locus_tag="ACFM8U_000703"
     CDS             8326..8523
                     /locus_tag="ACFM8U_000703"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6333981.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTAILLREMSGGGGFP"
     gene            8556..10193
                     /locus_tag="ACFM8U_000704"
     CDS             8556..10193
                     /locus_tag="ACFM8U_000704"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6333982.1"
                     /translation="MPAFFAVLAATLIGGFFLFTKDDFFLLWKSALTALGFASNLYFA
                     RGKDYFAPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVARKSLRVQADFLAALCALSL
                     ATSFIPSALDKYYLPHLRACEMLVGSLTAVWMRCRQPAVGRRCAAAGALFAVCILSTC
                     LFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKFFQWKITVAAGLISYSLYLW
                     HWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYHCIEKPFKKWKGSFAQSVLWIY
                     ALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQPELLVLG
                     DSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYVSRVFQNWAACRAVYRYAEEHL
                     PRYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHKLSSEKQAVYLMADNLASSY
                     NVQRAYILSSRIPGYRQALRPDDESTLKANARIRELAAKYPNVYIIDAAAYIPADFQI
                     GGLPVYSDKDHINPYGGTELAKRFSEKQRFLNTRHNH"
     gene            10255..11550
                     /gene="tyrS"
                     /locus_tag="ACFM8U_000705"
     CDS             10255..11550
                     /gene="tyrS"
                     /locus_tag="ACFM8U_000705"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC6333983.1"
                     /translation="MSVIQDLQSRDLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLNGEHKRFGKYTILRRGKRNHALLVWK"
     gene            12077..12997
                     /gene="ribF"
                     /locus_tag="ACFM8U_000706"
     CDS             12077..12997
                     /gene="ribF"
                     /locus_tag="ACFM8U_000706"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC6333984.1"
                     /translation="MKIRLGRHNAPDFPQGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAALAGKTPPCRISPLRTKLELLEGTGCVDAVWVLGFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPDHR
                     YALGGVFVVDADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIAADMEAAKCW"
     gene            13139..15928
                     /gene="ileS"
                     /locus_tag="ACFM8U_000707"
     CDS             13139..15928
                     /gene="ileS"
                     /locus_tag="ACFM8U_000707"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC6333985.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGISILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYSVVLARQLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFAMLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         3731 a         4368 c         4405 g         3678 t
ORIGIN      
        1 aaatcgtcag aaatacccga accgtcattc ccgcgcaggc gggaatccag acctgtcggt
       61 gcagaaactt atcgggtata acggtttctt gagattttac gttctagatt cccactttcg
      121 tgggaatgac ggcatcggtc tgcctttaca acacggtttc ttagattcca cgttctagat
      181 tcccgcctgc gcgggaatga cggcatatcg gcaggcgcgg ggcttgggac ggcattgctg
      241 ttgcggtttc ggacgcggct ttattcgacg acaattttag ggaaccggct gctgaagtct
      301 ttgcctttgt cggcaatgct cagggcgatt tggaatgcgg catcggtgta gattttggcg
      361 atggcggggt gttcgtcgaa cagttgcgcc ggtgtgccgc cgtccattgc ttcgcgcacg
      421 ggcaggctta ggggaagctg tccgagcagg gggacgttga ggcgggccgc caaatctttg
      481 ccgccgtccg tgccgaacag tgcttcgctg tgtccgcagt tggtgcagat gtggacggac
      541 atattttcca atacgcccaa aatgggaatg ttgactttgc ggaacatatc cacggctttg
      601 cgcgcgtcta tcagggcgat gtcctgcggc gtggttacga tgaccgatcc ggttacgggg
      661 atgcgctggg acagcgtgag ctggatgtcg cccgtacccg ggggcaggtc gatgaaaaga
      721 tagtccactt cgtcccactc gctttggaac atcagctgct gcaaggcttg gctgaccatc
      781 ggcccgcgcc agacgacggc ttggccggta tcgacgagaa agccgataga catgacctgt
      841 atgccgtctg aagattcgac gggaatgagt ttttggtttt tctgatcggg tttgcggtcg
      901 tccacaccca acatggtcgg ttggctcggg ccgtaaaggt cggcatcgag tacgccgacg
      961 cgcgcgccca tgcgcgccat tgcggcggca aggttggctg tcgtcgttga tttgcccacg
     1021 ccgcctttgc cagaggcgac ggcgatgatg tttttcacgc ctttgatggt ggtaacgcca
     1081 ggttggactt tgtgtgtgcc gatttcagtg tccatggaca gatggatgtg tgtgtcgcct
     1141 gtttcgggca tcaggatttc ctgtatgcgg tcggctgttt ctgaggcaat gtgcgcgacg
     1201 gggaagccga aatgcagggc gatatggatg ccgtctgaac gctgttcgac cgaacggacg
     1261 gccttttcgc cgccgagcgt gcgtgccgta ttcggaacgg cgacggtgtc gaggagggtg
     1321 cggatgtttt gtatgttcat aatgtgtgtt tctgtgtgca gtttgggaaa cgggcggaaa
     1381 gtgtacattg atttcgggcg gtttgccttt ccttgtgcga acttttacac ggtatgcggc
     1441 ggattgtgaa aatccgttgt caaaatatcc cgtgcaaaca gtggtctgca attaagcctg
     1501 taaaatttgc ttgacgaggt aggatggtgc tgtataatgc acggcttatc gggtcgttag
     1561 ctcagccggt agagcagcgg acttttaatc cgttggtcga gcgttcgaat cgctcacgac
     1621 ccaccagata accggaagcc aagttttgag acttggcttt ttgtttgcct gccggttgcg
     1681 cgggcgtacc gtttgagaga gcgtatgaaa aagaaactgc tttcgggcat caaatttgcc
     1741 gttcagacgg cattggtgtt tttattggtg tcgctgtttt tggattggat acgcaagccc
     1801 gaagagccgg ccggtgcggc agggcggcct ttgaccctgc tgtcggggca gcggctgact
     1861 ttgggacagt ttagccgaga taaggcggtt ttggtgtatt tttggggaag ctggtgcggc
     1921 gtgtgccgtt atcagtcgcc gataatcgat gatttggcgg cggacggcgt tccggtcgtc
     1981 ggcgtggcgg tacgttccgg cagcgcgtcc gaagtggcgg cgtatatggc aaagcggggc
     2041 ttgggctttc cgacggtcaa cgatgaggac gggggtttgg caaggtcttg gcggattgtc
     2101 gcaacgcctg ccgtcgtttt ggtcaaaaat gggaaaatgg ttcactatac gacgggaatc
     2161 agcagttatt ggggcttgcg cgcacgaatt tttcaggcgg atgttttcgg ttaacttttg
     2221 tttttgttaa actttacgct atgtagagaa tcaaacggca tataccttgc tcgtctgatg
     2281 cggcggcttt tgttttttct ggaagaaagt tctttcgaca gtaaatgtaa tactttgttg
     2341 agattgtagg gaaaatagac taagatataa ctattaaaat atttttagat aggattatcg
     2401 gaattaaagt cttttatacc cagtcgtccg atgcggttta tagcgtattg ttgctatatg
     2461 ttcgttttgt tatataacgg ttgcatcaaa aattacgccc acaggctttc ccgacggttt
     2521 gaaagtttga ttttcgataa cttggagact tgaacaatgc ctacccaatc aaaacatgcg
     2581 tctatcaata tcagtctgat acaggcaagg gaagccctga tgacccaatt caggcctatt
     2641 ctgaatcagg cgaatattac cgatcagcaa tggcggatta tccgtctttt ggcggaaaac
     2701 ggcacgctgg actttcaaga tttggcgaat caggcgtgta ttctgcgccc cagcctgacc
     2761 ggtatcctga cccgccttga aaaagcgggt ttggttgtcc gcctgaaacc ttccaacgac
     2821 caacgacgtg tttttctgaa gctgactgcc gagggcgaga agctgtatga ggaaatcggc
     2881 gaagaagtgg acgagcgtta cgacgctatc gaggaagtgc tgggccgcga gaaaatgctg
     2941 ctgcttaaag acctgttggc agaacttgcc aaaatcgagg atgcgttgaa ctcgtaatac
     3001 gccgtaacgc gcggaaacgt ccgaccgacg gctttttgaa tcagaactgc tgcacatggg
     3061 gggtgccttg tgtgcagcat tcttatatag gggacagttt aaaggggaaa aatggcggat
     3121 ttgcagaaaa cttttcaaac ttcgttccgt gatgcgatgg catcttgcgc ggcaggcgtt
     3181 catgtcatca cgacagacgg tgcggcaggg cgttacggca ttacaatgac ggcggttgcg
     3241 ccggtaacgg acgagccgcc gaccgtgatg ctgtgcatca accgtaaagc ccgtatcatc
     3301 ccgatactgt cggaaaacgg cagcctgtgc atcaatacgc tggcggacga acatcaggat
     3361 gttgccgaac attttgccgg gctgaccggc ctgtcgcccg aagagcggtt tgcctaccac
     3421 atctggcatc gcggcaaaac gggacaactt gaaatagaag gagcgttggc gcacctgcac
     3481 gggcatattg tcggcaaaca tgaaatcggc acgcattttg tgttttacgt caggctcgac
     3541 gaaattaaaa actgcgggtg caaacgtccc gcgctgctgt atttcagacg gcagtttaga
     3601 tttttagact gatattcgga cagatatatg aaagcgatga tactggcggc aggacgcggc
     3661 gagcgtatgc gccctttgac cgataccact ccgaagccgc tgcttgatgt ggcgggtaag
     3721 cctctaatcg gttggcacct atgccgtctg aagcaggcgg ggtttaccga aatcgtcatc
     3781 aaccacgctt ggctgggtcg gcagatagaa gatgctttgg gcgacggctc ggcttatggc
     3841 gtgaacatcg cctattcgcc cgaacccgca ggcggtttgg aaacggcagg cggcatcgcg
     3901 caggcattgc cgctgttggg tgggcagccg tttttggtgg tcaacggcga cgtgctgacc
     3961 gacatcgatt ttaccgccgc gtttcagacg gcatcgtccc tgccggaaca tatttccgcc
     4021 catctgtggc tggtggaaaa tccgccgcac aaccccgacg gcgatttttc cctgctgccc
     4081 gacggcagcg tgcggccgga agtaaatggc ggcaacggat tgacatttag cggcgtgggt
     4141 atttaccgtc ctgaaatgtt tgatggaatc gaagcgggca gtgtggcgaa actcgcgccc
     4201 gtattgcggg gcgaaatgcg gcaaaaccgc gtcagcggac agaagcatac gggcttgtgg
     4261 ctggatgtcg gcacggtatg ccgtctgaaa gaggctcaag cccttgcagg ggcttggaag
     4321 tgaaataaaa cggtggcttg ccgagccggc tgccgaacgg ttccgaccgg atgtccggca
     4381 tattttcgtg gaggcgtgaa aatagggcgt tcggggcttt ccgtccaaca aatgccatct
     4441 gaagccgacc ctgtcggatg cggcggtttg ccgtcggcgc ggtgtcggat tttattgggt
     4501 aactttgttc agaccgccgc ccgtcagatt gtcaaaaccc ggtttcaggc ggtatggcgg
     4561 attcggttta acgtttcaac gccagcacca acacgcccgc cgttaccagc cccaagccta
     4621 tccattcctg cgtgttcggg cgttcgtcca agaaaaccac cgccatcagc gcgaccagaa
     4681 ccaggctgaa tttatcaacg ggggcgactt gcgaggcgtt acccagttgc agggctttaa
     4741 aataggcgag ccaggatgcg ccggtggcga gtccggataa aatcagaaac gtccagttgc
     4801 ggccggtaaa gccgtttacg ccctgccatt tgccggtgta ggttaaaaac aataccaaag
     4861 cggcgaggat gaccaaggtg cggatgaaag tggcaaaatc ggaatctatt ccttgtaaac
     4921 ccattttggc gaaaacggcg gtcaatgagg cgaagcctgc cgatgccaat gcccaaaaca
     4981 gccatgcgtt gctgcccatg ttttctcctt ggattgtgaa caatatgaac ggtatttttg
     5041 ttgcagcgtc aaaaatttca ctgcgggttt ggtgcggata acgttataat atgcctgata
     5101 ttattttcaa tccacctgtt tgtcgcctga tgctttcaga cggcatgtcc ctcctcattt
     5161 ctaaaggaaa atcatgagct tcaaaaccga tgccgaaatc gcccaatcct ccaccatgcg
     5221 cccgattggc gaaattgccg ccaagctggg tttgaacgtt gacaacattg agccttacgg
     5281 tcattacaag gcgaaaatca atcctgccga agcgttcaaa ctgccgcaaa aacagggcag
     5341 gctgattttg gttaccgcca tcaacccgac tccggcgggc gaaggcaaaa ccaccgtaac
     5401 catcggtttg gcggacgcgt tgcgccacat cggcaaagat gccgtgattg ccctgcgcga
     5461 accttctctg gggccggtgt tcggcgtgaa aggcggcgcg gcaggcggcg gctatgccca
     5521 agttttgccg atggaagaca tcaacctgca cttcaccgga gattttcacg ccatcggtgc
     5581 ggcaaataat ctgcttgccg cgatgctcga caaccatatc tatcagggca acgagttgga
     5641 catcgacccc aaacgcgtgc tgtggcggcg cgtggtcgat atgaacgacc gccagttgcg
     5701 caacatcatc gacggcatgg gtaaacccgt tgacggtgtg atgcgccccg acggttttga
     5761 tatcaccgtt gcctccgaag tgatggcggt attctgcctt gccaaagaca tcagcgattt
     5821 gaaagagcgt ttgggcaaca tccttgttgc ctacgccaaa gacggcagcc ccgtttacgc
     5881 caaagatttg aaagcacacg gcgcgatggc ggcattgctt aaagatgcga ttaagcccaa
     5941 cttggtgcaa accatcgaag gcacacctgc cttcgtacac ggcggcccgt tcgccaacat
     6001 cgcccacggc tgtaactctg taacggctac ccgactggcg aaacaccttg ccgattacgc
     6061 cgtaaccgaa gcaggcttcg gcgcggattt gggcgcggaa aaattctgtg acatcaaatg
     6121 ccgtctggcg gatttgaaac ctgatgcggc tgttgtcgtc gctaccgtcc gcgctttgaa
     6181 atataacggc ggcgtggaac gtgccaacct aggcgaagaa aacctcgaag ccctggcaaa
     6241 aggtttgccc aacctgctga aacacatttc caacctgaaa aacgtattcg gactgcccgt
     6301 cgtcgttgcg ctcaaccgct tcgtgtccga ctccgatgcc gagttggcga tgattgaaaa
     6361 agcctgtgcc gaacacggcg ttgaagtttc cctgaccgaa gtgtggggca aaggcggcgt
     6421 gggcggtgca gacttggcgc gcaaagtcgt caacgccatc gaaaaccaac cgaacaactt
     6481 caatttttcc tacgatgttg agctgagtat taaagacaaa atccgtgcga ttgcccaaaa
     6541 agtgtacggc gcggaagatg ttgatttcag cgcggaagcg tctgccgaaa tcgcctcgct
     6601 ggaaaaactg ggtttggaca aaatgccggt ctgtatggcg aaaacccaat attctttgag
     6661 cgacaacgcc aaactgttgg gctgccccga aggcttccgc atcgccgtgc gcggcatcac
     6721 cgtttccgca ggcgcaggtt tcatcgtcgc cctgtgcggc aacatgatga aaatgcccgg
     6781 cctgcccaaa gttccggctg ccgagaaaat cgatgtggac gcagaaggcg tgattcacgg
     6841 cttgttctga acggttttct gaaaccggat gccgtctgaa gccgtttcag acggcatttt
     6901 ttcggaacgc gggcggcggt atgctataat ccgccgttaa atttctctat tttcaggaaa
     6961 aaacatgagt ttgaaatgcg gcatcgtcgg tttgcccaac gtcggcaaat ccaccctttt
     7021 taacgcgctg acccaatcgg gtatcgaagc ggcaaactat cctttctgta ccatcgaacc
     7081 caacgtcggc atcgtcgaag tccccgatcc gcgtatggcc gaattggcaa aaatcgtcaa
     7141 tccgcaaaaa atgcagcctg ccatcgtcga atttgtcgat attgccggtt tggttgcagg
     7201 cgcgagcaaa ggcgagggct tgggcaacca gttccttgcc aacatccgcg aaaccgatgc
     7261 gattgtgaat gtcgtgcgct gctttgacga cgacaacatc gtccacgttg caggccgcgt
     7321 cgatccgatt gccgacattg aaaccatcgg tacagagttg gcccttgccg acttggcaag
     7381 tgtggaaaaa gccatcgtcc gcgaagaaaa acgcgcccgt tccggcgaca aagacgcgca
     7441 aaaactggtc gaactgtgta aaaaactgct gccgcatctg gacgaaggca aacccgtgcg
     7501 ttccttcggt ttggacgcgg aagaacgcgc gatgctcaaa ccactgttcc tgctgaccgc
     7561 caaaccggcg atgtatgtgg gcaacgtcgc cgaagacggt tttgaaaaca atccgcacct
     7621 cgaacgcctg aaagaattgg cggcaaaaga aaacgccccc gtcgtcgccg tttgcgccgc
     7681 aatggaaagc gaaattgccg aattggaaga cgacgaaaaa gccgaattcc tcgccgaaat
     7741 gggcttggaa gaacccggcc tgaaccgcct gattcgtgcc ggttacgacc tcttggggct
     7801 gcaaacctat ttcaccgccg gcgtgaaaga agtccgcgcg tggacgatac acaaaggcga
     7861 caccgccccg caagccgccg gcgtgattca caccgacttt gaacgaggct tcatccgcgc
     7921 ccaagtcatt tcttacgacg actttgtctc gctcggcggc gaagccaaag ccaaagaagc
     7981 cggcaaaatg cgtgtggaag gcaaagaata cgtcgtgcaa gacggcgatg taatgcactt
     8041 tttgtttaac gtgtaaccca aatgcggcag gttttcagac ggctttgccg gaaatgccgt
     8101 ctgaagccgg ttttggtggt tttcggcgtt tccataccgc cggaatgcgg ccgcatcaaa
     8161 ataaaatccc gcccgcattt ccgatttgcc ctccccgatt cctgcaaaac aaaccgcctg
     8221 ccccgctgtt gcaaaaatcc gtccggcatt ccgaatatcc cgagccccga tacaaaatga
     8281 cctttcagat ggcatttgcg ccgccgcgat gaaagcaaaa atattatgag ccaagcctta
     8341 ccctaccgcc cggacatcga cacattgcgc gccgccgccg tcttgtccgt catcgtgttc
     8401 catatcgaaa aaaattggct gccgggcgga tttctcggtg tcgatatatt ctttgtgatt
     8461 tcaggctttt tgatgacggc gatcctcctt cgcgaaatgt cggggggggg aggttttccc
     8521 tgaaggcatt ttatatccgc cgcatcaaac ggattttgcc cgcatttttc gccgtattgg
     8581 cggcaacgct gataggcggc ttctttttat tcaccaaaga tgatttcttt cttttatgga
     8641 aatccgcgct gaccgccttg ggtttcgcat ccaacctgta ttttgcaagg ggcaaggact
     8701 atttcgctcc cgcgcaggaa gaaaagcccc tgctgcacat ttggtctttg tcggtcgaag
     8761 aacaatttta ctttgtcttt ccgatattgc tgctgcttgt cgcccgcaaa agcctgcgcg
     8821 tccaagccga cttcctcgcc gcactgtgcg cgttaagcct tgccacttcc tttatacctt
     8881 ccgcgctcga taaatattac ctgccccacc tgcgcgcctg cgaaatgctg gtcggatcgc
     8941 tgaccgccgt gtggatgcgc tgccggcaac ctgccgtcgg cagacgctgt gccgccgccg
     9001 gcgcattgtt tgccgtgtgc atattgtcaa cctgcctgtt ttcctattcg gaacaaaccg
     9061 cctatttccc gggccccgcc gctttgattc cctgtctggc tgttgccgcg ctgatttatt
     9121 tcaatcatta cgaacacccg cttaaaaaat ttttccaatg gaaaatcacc gttgccgccg
     9181 gtttgatttc ctattcgctt tatctgtggc attggccgat attggccttt atgcgctata
     9241 tcggcccgga caacctgccg ccttattcgc cggcggcagc aatcgtcctg actctggcgt
     9301 tttccctgat ttcttatcac tgcatcgaaa agccgtttaa aaaatggaaa ggttcgttcg
     9361 cacaatccgt tttatggatt tatgccttgc ctatgctcat tttgggggcg ggctcgtttt
     9421 ttgcgatgag actgccgttt atggcgcaat acgaccgctt ggggctgacg cgttccaaca
     9481 cctcctgcca caacaatacc ggcaaacaat gcctgtgggg ggatacggaa aaacagccgg
     9541 agctgctggt tttgggcgac tcccacgccg accattacaa aacattcttc gatgccgtgg
     9601 gcaaaaaaga aaaatggtcc gccactatgg tttccgccga cgcttgcgcc tatgtggaag
     9661 gctacgtgtc ccgcgtgttc caaaactggg ccgcctgccg cgccgtttac cgctatgccg
     9721 aagaacacct gccccggtat tcaaaagtgg ttttggcgat gcgctggggc agccaaatgc
     9781 ccgaaaacag ccgctccctt gcctacgatg ccggtttttt ccaaaaattc gaccgtatgc
     9841 tgcataaact ctcgtccgaa aaacaagccg tttacctgat ggcggacaac cttgcctcgt
     9901 cttacaacgt ccagcgcgcc tatatcttgt cttcacgcat accgggttac cgccaagccc
     9961 tgcgcccgga cgacgaaagc accctgaaag ccaatgcacg catcagggaa ttggcagcca
    10021 aataccccaa cgtctatatt attgatgccg ccgcctatat ccccgcagat tttcaaatcg
    10081 gcggattgcc ggtttactcg gacaaagacc acatcaaccc ttacggcggc acggaattgg
    10141 caaagcgttt ttccgaaaaa caacggtttc tcaatacgcg ccataaccat tgattcgctt
    10201 aaatttgtta caatcggcgg tttgcaaaaa cgctaatttt tttgaaagag accgatgagc
    10261 gtcatccaag acctgcaatc gcgcgacctt atcgcgcaaa cgaccgacat cgaagcctta
    10321 gacgctttgt tgaacgaaca aaaaatcgcc ctttactgcg gtttcgatcc gaccgccgac
    10381 agcctgcaca tcggacacct gctgcccgta ttggcattgc gccgcttcca acaggcgggg
    10441 catacgccga ttgctttggt gggcggcgcg accggtatga tcggcgaccc cagcttcaaa
    10501 gccgccgaac gcagcttgaa ttccgccgaa actgttgccg gctgggtgga aagtatccgc
    10561 aaccaattaa cccctttctt gagctttgaa ggcggaaatg ccgccattat ggcgaacaat
    10621 gccgactggt tcggcagcat gaactgcctt gacttcctgc gcgacatcgg caagcatttc
    10681 tccgtcaacg ccatgctgaa caaagaatcc gtcaaacagc gcattgaccg cgacggcgca
    10741 ggcatttcct tcaccgagtt cgcctactcc ctgctgcaag gctacgactt cgccgagttg
    10801 aacaaacgcc acggcgcggt tttggaaatc ggcggctccg accaatgggg caacatcacc
    10861 gccggtatcg acctgacccg ccgcctgaat caaaaacaag tgttcggcct gaccctgcct
    10921 ttggttacca aatcagacgg caccaaattc ggcaaaaccg aaggcggcgc ggtgtggctg
    10981 aacgcgaaaa aaacctcgcc ctatcagttc taccaattct ggctgaaagt cgccgatgcc
    11041 gatgtgtata aattcctgaa atactttacc ttcctgtcca tcgaagaaat cgacgctatc
    11101 gaagcgaaag ataaagccag cggcagcaag cccgaagcgc aacgcatcct cgccgaagaa
    11161 atgacccgcc tgattcacgg cgaagaagcc cttgccgccg cgcagcgcat ttccgaaagc
    11221 ctgtttgccg aagaccaaag cagcctgacc gaaagcgact tcgagcagct cgccctcgac
    11281 ggcctgcctg catttgaagt ttcagacggc atcaatgtcg tcgaagcctt ggtcaaaacc
    11341 ggtttggcat cctccaataa agaagcgcgc ggctttgtga acagcaaagc ggttttgctc
    11401 aacggcaaac ctgccgaagc caacaacccc aaccacgccg ccgaacgccc cgacgatgcc
    11461 tgcctgctga acggcgaaca caaacgtttc ggcaaataca ccatccttcg gcgcggcaaa
    11521 cgcaaccacg cgcttttggt ttggaaataa tccgattgcc gcagaaatgc cgtcattccc
    11581 gcgcaggcgg gaatccggac ctgtccgcac ggaaacttat cgggcaaaac ggtttcttag
    11641 attccacgtt ctagattccc gcctgagcgg gaatgacgag tttcaagatt acggtgttgt
    11701 cggaacgcaa ctgaaccgtc attcccacga aagtgggaat ctagaatctc ggggtttgag
    11761 caactgtttt tatccgataa gtttctgtgc ggacaggtcc ggattcccgc ctgcgcggga
    11821 atgacggcgg agggttgctt gtctcggttt acctggttaa aaaagaacga ttttcactga
    11881 tgttgcatca ggtttggggc gatgtttcaa cacataggat cgcgcctgat gcacgcttta
    11941 tgcgtttggc gcgttcggcg gcgggaaatt tgcctgcttt tccagcgtcg ggcgggcgta
    12001 acgggcggca cactgtctat aaaccgcaat accgtttaca atgaccgcct gtttcaccac
    12061 atacccgaac gcaacaatga aaatcaggct ggggcggcac aacgcgcccg actttccaca
    12121 gggtgccgcc gtaaccatag gcaatttcga cggcgtacac ctcggacaca aacacatcct
    12181 ccaaaaactc cgcctcgaag ccgacgcgcg cggactgccc gtcgttgccg tcatcttcga
    12241 acctcaaccc aaagaatttt ttgccgccct cgcaggcaaa accccaccct gccgcatcag
    12301 ccccctgcgc accaagctcg aattattgga aggcacaggc tgtgtcgatg ccgtctgggt
    12361 tttgggtttc gatcaaaatt tttccgaaat atccgcgcaa gggtttatcg accgcctgct
    12421 gcgtcaaacc ttgaatacgc gttatttgct cgtcggcgac gatttccgtt tcggtgcggg
    12481 gcgggaaggc tgttttgaac ttttggcaca acagcccgat atgcagaccg aacgcacgcc
    12541 ttccgtcatc gtcgaagaca tccgcaccag cagtaccgcc gtccgccaag ccctttcaga
    12601 cggcaacctc gcttatgcga aaaaactttt gggacacgac tacgttttgg ggggcagggt
    12661 ggtgcacggc agaaaactcg gacgcaccct aaacgccccg actgccaaca tccgcctgcc
    12721 cgaccaccgt tatgccctcg gcggcgtgtt cgtcgtcgat gcggacggcg cattcggcac
    12781 gcggcgcggc gtggcgagct tcggcttcaa tcccaccgtt gatagcggct gttctcaaaa
    12841 gcttgaagtc cacctgttcg actttcaagg cgacctgtac ggacaacggc tgaacgtccg
    12901 cttcctgcac aaactgcgcg atgaggaaaa gtttgacggt atggaagaac tgaaaaggca
    12961 gatagcagcc gatatggaag ccgcaaagtg ttggtagaaa aaccttatac aaaccatccg
    13021 attgggctac aattaatctt ttaacttttc agacggcata ggggtttccc gttgtgaaat
    13081 actgtttgag gcgcaatgcc gtctgaaacc gaaatattgt aacaatagag attaaaaaat
    13141 gaccgattac agtaaaaccg taaacctgct cgagagcccg tttccgatgc gcggcaacct
    13201 tgccaagcgc gagcctgcat ggctgaaaag ctggtacgag caaaaacgct accaaaaact
    13261 gcgcgaaatc gccaaaggtc gtccgaagtt catcctgcac gacggcccgc cgtatgccaa
    13321 cggcgacatc cacatcggtc atgccgtcaa taaaattctc aaagacatca ttatccgcag
    13381 caaaacccaa gccggttttg acgcgcctta tgtaccgggc tgggactgcc acggcctgcc
    13441 tatcgaagtg atggtggaaa aactgcacgg caaagacatg cctaaagccc gtttccgcga
    13501 attgtgccgc gaatacgccg ccgaacagat tgcccgtcag aaaaaagact ttatccgctt
    13561 gggcgtgttg ggtgactggg accatcctta cctgaccatg gatttcaaaa ccgaagccga
    13621 taccgtgcgt atgctcggcg aaatctacaa atccggctat ctctaccggg gtgcgaaacc
    13681 ggttcaattc tgcttggatt gcggctcttc attggccgaa gcggaagtgg aatataaaga
    13741 caaaatctcg cccgcgattg acgttgccta tccgtttaaa gacaccgccg cgcttgccgc
    13801 cgcattcggt ttggcaggta tcgaaggcaa agcgtttgcc gttatttgga caaccacgcc
    13861 ttggactttg cctgcgagcc aagctgtttc agcgggtgca gacgtggtgt atcaattaat
    13921 cgatacgccc aaaggcaaat tggtattggc caaagatttg gcggaagacg ctttgaaacg
    13981 ctacggcttt tcagacggca tttctattct tgccgaaacc accggcgaca agctggaaaa
    14041 cctgcacatg aaccatccgt tcctcgaacg cgatattccc atgcttaacg gcgaacacgt
    14101 taccaccgat gccggtaccg gcttggtgca caccgccccc gcgcacggtt tggaagacta
    14161 cgccgtctgc aacaaatacg gcatcgagct ttacaatcct gtcaacgccg aaggcaaata
    14221 catcggcgaa acgccgcgcg ttgccggtat gcgcgtttgg gaggcgaacc ccgtcatcct
    14281 gcaatggttg gaagaaaccg gcaacctttt ggcaagcagt aaaatcgaac acagctacgc
    14341 ccactgctgg cggcacaaaa cgccgctgat ttaccgcgcg accggtcaat ggtttgtcgg
    14401 tatggacaaa gccggtgccg acggcaaaac cctgcgcgac aaagccatca aggccgtgga
    14461 cgacaccgaa ttcttcccgt cttggggtcg cgcgcgtttg gaagccatga ttgaaggtcg
    14521 tcctgactgg gtggtttcac gccaacgcta ttggggcacg ccgatgactt tctttgttca
    14581 caaagaaacg ggcgagctgc atccgaactc tgccgaactt ttggaaaaag ttgccctgaa
    14641 aatcgaagaa aaaggcatcg aggcttggtt ctccctcgat aaaagcgaat tattaagcgc
    14701 ggaagattgc gaacattacg acaaactccc cgacacgatg gacgtatggt tcgactccgg
    14761 ttcgacccat tattccgttg tgaaacagcg cgaagaattg gactggcctg ctgacttgta
    14821 cctcgaaggc agcgatcaac accggggctg gtttcagtcg tccatgctga ccggctgcgc
    14881 ctcatcaatg ggtcgcgcgc cgtacaaaca gctgctgacc catggtttcg tggtggatca
    14941 aaacgggcgc aaaatgtcga aatccatcgg caacgtcgtc gcgccgcaag aagtgtataa
    15001 cgagttcggc gcggacatcc tgcgcctgtg ggcggcatcc accgattaca gcggcgaatt
    15061 ggcgatttcc aaagaaatcc tcaagcgcgt aaccgaaagc taccgccgta tccgcaatac
    15121 cttgagcttt ttgtttgcca acttgagcga cttcaatcct attgaagatg ccgtgcaaca
    15181 ggcggatatg gtggaaatcg accgctactc cgtggtattg gcacgtcagc tgcaagagcg
    15241 tctggcaggc gattactatc cgcgttatgc cttccacttt gccgtaaaag acattgtttc
    15301 tttctgctcg gaagacttgg gtgcgttcta cctcgacatc ctgaaagacc gcctctacac
    15361 caccaaagcc gacagccatg cacgccgcag cgcacaaact gccctgtatc acatcacacg
    15421 cagtttggtt ctcttgattg caccgatttt gtgcttcacc ggcgaagaag cgtgggacat
    15481 catcggcggc ggcgaagaag acagcgtcct cttccatact tggcacgagt tcccgaccat
    15541 caacgaaaaa accgaagccg aactggtgaa aaaatggacg gcaatccgcg aagcccgcga
    15601 agcggtaacc gccgccatcg agcctttgcg cgccgacaaa accgtcggtt cgtccttgca
    15661 agccgaagcc gaaatcaccg cgccggaaga aatggccggc tacctgaacg ccttgggcga
    15721 agaattgcgc tttgccatgc tggtgtctaa agcagaagtg aaagtaggca gcgaacttgc
    15781 cgttgccgct aaagccagtg atggtgaaaa atgcgaacgc tgctggcact acacccgcga
    15841 tgtgggcgcg gttgcaggct atgaaaccgt ctgcaaacgc tgtgcagaga atgtcggcgg
    15901 agaaggcgaa acgcgccatt acgcctgata aagtttgagc aaatgccgtc tgaaaccgcc
    15961 gaacagcatt tcagacggca ttttttgtgc cgcgatttgt ctttataacg gcggaggggt
    16021 ttcaagattg cggtgttgtc ggaatgcaac tgaaccgtca ttcccacgaa agtgggaatc
    16081 tagaatctcg aagtttcagt catttccgat agattcccgc cgcgtcgtgg gtctggattc
    16141 ccgcctgtgc gggaatgacg gatttcgaga ttgcggtgtt gt
//
LOCUS       DAXHSJ010000030        16066 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000030 DAXHSJ010000000
VERSION     DAXHSJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16066
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.030"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(26..124)
                     /locus_tag="ACFM8U_000708"
                     /pseudo
     CDS             complement(26..124)
                     /locus_tag="ACFM8U_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
     gene            complement(229..726)
                     /locus_tag="ACFM8U_000709"
     CDS             complement(229..726)
                     /locus_tag="ACFM8U_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HFC6333986.1"
                     /translation="MNVKVHKDTHHFGIHNLNNTDVHLTAEDSDNVIEIKGELADLMP
                     TGNVESTSFLRGIETAFKSNTVLTGNNNKISIATEGKGLGLGVNGLQSNSPYSGHTTG
                     IFINNQSSDLTQPSLDGISTIKLKAVKGNNEVDLNVKNHASVKGIIASHSAKATLEAE
                     GDNIV"
     gene            complement(812..1234)
                     /locus_tag="ACFM8U_000710"
     CDS             complement(812..1234)
                     /locus_tag="ACFM8U_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333987.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNFQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            1546..2856
                     /locus_tag="ACFM8U_000711"
     CDS             1546..2856
                     /locus_tag="ACFM8U_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC6333988.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLAQILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            2970..5975
                     /locus_tag="ACFM8U_000712"
     CDS             2970..5975
                     /locus_tag="ACFM8U_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFC6333989.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNENWDKPTNIFPKI
                     FIESLCRINPDLSPDDARRLLTDIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEEFRPDITLLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     SKFRRFISITQFMIFSNNMEYDDGATEPAQGAFYASSAYGKPVFNYFREEHKLNLAEL
                     LNTLSDGLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLSFLLQHGL
                     TYVKASQGLVQKHIMRYPQLFATLAIEKHLANGGKKGVIWHTQGSGKTALAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLVVHTIDSREAFAADIKSAQTLHN
                     HAGKAEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDDSLGAMVVC
                     DSAEQARQLFEHFQIASDHNFTAALILHDVGTKDERDQWVKDFKAGKIDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSRLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIADKK
                     QLLALKKALQTAKKLYNVLRLQGSHEFLAHLDFDKLNLLYRETAARLETLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQEDPEYISLR
                     EELERIFKKKNLAEVGQEEMQANIAILETVYAKIKELNRQNDLLRHKYGGDAKYARIH
                     KRLRENPALYGDKLKVFNALNGVKTDTDQKVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQAINRLLVREYLKESGRI"
     gene            6024..7013
                     /gene="rhuM"
                     /locus_tag="ACFM8U_000713"
     CDS             6024..7013
                     /gene="rhuM"
                     /locus_tag="ACFM8U_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HFC6333990.1"
                     /translation="MYNNDIIIYTTEDGLSEFTLRELDGELWLTQKEIAELYQTSKQN
                     IGKHIKAIFAEQELDDSVVNFQFTTAADGKNYRMGLYPLSLIIAVGYRVRSTRGTQFR
                     QWATRTLGEYISKGFVLNDDRLKNPPVGTNRAEDQFEQLLNRIRDIRSSERRMYLRVR
                     EIFAMAADYQPSFKETTHFFKIIQNKLHYACTRYTAPEIIYQRADAGKPNMGLTHWRG
                     KEIAKADVTVAKNYLNEAEIDALNRIVSMWLDFAEDQASMKKQIFLQDWAEKLDAFLA
                     FNDRQVLQTAGTISKTQADEKARIEYEKFAAARRLEKEREGERHIAELLNLKK"
     gene            7107..8264
                     /locus_tag="ACFM8U_000714"
     CDS             7107..8264
                     /locus_tag="ACFM8U_000714"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6333991.1"
                     /translation="MNSALKNHATYINNKVDTNEISLSQYISTDNILQNKQGIECATS
                     LPIQGGKVTSFKKGDILLANIRPYLKKIWYAQFDGGCSADVLAIRANTETDSHFLFYA
                     LFRDDFFIHAMKGAKGTKMPRGDKTQIMEFKIPVFDLKTQQSIAAVLSTLDKKIALNK
                     QINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGEIVFDETLKREIPKGWGSIELQ
                     SCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNPQDAHIIFGDHTR
                     IVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHFKFLKEFKII
                     LPSKDISQKYNEIANTFFVKVRNNIKQNHQLTQLRDFLLPMLMNGQVSVAE"
     gene            8312..9913
                     /locus_tag="ACFM8U_000715"
     CDS             8312..9913
                     /locus_tag="ACFM8U_000715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC6333992.1"
                     /translation="MTEQHFTEQTKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKQIRKENPDEPIEFINMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAAHNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPAHKDASGRLKKFDFIVSNPPFKLDFSDFRDQLEGEENRERFFAGIPKIKAKD
                     TDKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAQSGIDKKIREHLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKANKDKVVLIDASGLGEKIKDGKNQKTVLSREEEQKICN
                     TFTHKQAVEDFSVVIGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            9945..10061
                     /locus_tag="ACFM8U_000716"
     CDS             9945..10061
                     /locus_tag="ACFM8U_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6333993.1"
                     /translation="MPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYRK"
     gene            complement(10221..11558)
                     /gene="ribBA"
                     /locus_tag="ACFM8U_000717"
     CDS             complement(10221..11558)
                     /gene="ribBA"
                     /locus_tag="ACFM8U_000717"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC6333994.1"
                     /translation="MTDTAGLRRHNLRQWIEKYYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPSEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            11666..11797
                     /locus_tag="ACFM8U_000718"
     CDS             11666..11797
                     /locus_tag="ACFM8U_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333995.1"
                     /translation="MPSEAGFVASDGIAAIPAGGAAVSSRPLPIRGLPEAYSEKNFW"
     gene            complement(11773..12429)
                     /locus_tag="ACFM8U_000719"
     CDS             complement(11773..12429)
                     /locus_tag="ACFM8U_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HFC6333996.1"
                     /translation="MILHPSSLNYHVIPKGADFSDNDFVRHFETLVEGRYYIANAWTK
                     IVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGGSISHNIK
                     YKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNIILIY
                     RKYFSFKEKCRKIFGAKAIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKFFSE
                     "
     gene            complement(12426..12787)
                     /locus_tag="ACFM8U_000720"
                     /pseudo
     CDS             complement(12426..12787)
                     /locus_tag="ACFM8U_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(12955..13548)
                     /gene="ribA"
                     /locus_tag="ACFM8U_000721"
     CDS             complement(12955..13548)
                     /gene="ribA"
                     /locus_tag="ACFM8U_000721"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC6333997.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(13541..14218)
                     /locus_tag="ACFM8U_000722"
     CDS             complement(13541..14218)
                     /locus_tag="ACFM8U_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6333998.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNIASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            14372..14456
                     /locus_tag="ACFM8U_000723"
     tRNA            14372..14456
                     /locus_tag="ACFM8U_000723"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14406..14408,aa:Leu,seq:tag)
     gene            14977..16011
                     /gene="purM"
                     /locus_tag="ACFM8U_000724"
     CDS             14977..16011
                     /gene="purM"
                     /locus_tag="ACFM8U_000724"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC6333999.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGVGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         4412 a         3955 c         3773 g         3926 t
ORIGIN      
        1 cgaggcaacg ccgtactggt ttttgttaat ccactataaa ctgacgcaaa tgccgttttg
       61 tacaattcca aaagttttca attccgttaa tgcgattttg ccgtttggcg aaatgcgtac
      121 tgttccagtc gtggattgaa cccccaccct gtatagttct ttcgaagcat tggggtattg
      181 ttttttcaaa gcatcgatta aagccgtatt ggtgtccgga tttttaacct agacaatatt
      241 gtccccttcg gcttcaagcg ttgcttttgc agaatgcgac gcgataatgc cttttacgga
      301 ggcgtggttt tttacattta aatcaacttc attatttccc ttgacggctt tgagcttgat
      361 ggtgctgatg ccatcaaggg aaggttgggt aaggtcgctt gattgattat tgatgaaaat
      421 gcccgtcgtg tgtccggaat agggggagtt ggactggaga ccatttacgc ccaatcccaa
      481 gcccttgccc tcggtggcga tggatatttt attgttattc cccgttaata cggtattgga
      541 tttaaaagcc gtttcaatgc cccttaaaaa acttgtactc tcaacatttc ctgtcggcat
      601 aaggtcggcg agttcccctt taatctcaat cacattatcg ctgtcttcgg cagtcagatg
      661 aacatcggta ttgttgaggt tgtgaatacc gaaatggtgt gtgtctttat gaactttaac
      721 gttcatattg aacaaattgc ctgtttttga atgaaaggat atgctgcctt tgtcattttc
      781 atcggggggc tgatagtgtt gacataccgc actaaggcac tattgtttgt cggtattttt
      841 ttccactggc tacatcttgt accccatcaa catcgggaac attcaaatta aattgaattt
      901 ttcctttggc atccaaatgg atatttccgg aatggggctt cacgtcaaca gattggaaat
      961 tatttttaaa tagaatatca ttattgcttt ttaccaataa ttcaggcgta tatcccaatt
     1021 gagagtctgc aaaagattgg atgccgtaaa ttccatttac cgttaaattt ccattatcat
     1081 ttttaccagt ggtaataagt tctaattttc taaagttttc accttttttc caccaaggca
     1141 caatatagtt ttttacagtg attttttcat ctttaagggt tataagacta tcatcgtctc
     1201 tttgcggatc agaaaataaa gacacccatt ccatctcttg aatattagta tattcatggg
     1261 cttccgcctc aaaacataat gccgattgaa tcaacaaagc aatggtagat aaagcaaatt
     1321 ttctatttta gtctccttaa tgtttaattg aaacgctgat ttgggtattg tcatgccggt
     1381 gcggaatcgg tctccgtgcc ggatatattg tgtgaagtct ctcgtctttt ccagtatgcg
     1441 cctaatatcg gcttaaggca aacggcgggg cggaatccgt atgcggcacg ccgccgttcc
     1501 ctgccgtata atcatattgt ttgaaaacct gttgacggag ccgttatgac cgatttgttt
     1561 gcccgagaac ccgacgcgcc gcttgccgaa cgattgcgtc cgcatacgct tgacgacgtg
     1621 gtggggcagg aacacctcat cggcgaaggt aaacctttgc gcgtggcggt agaaggcggc
     1681 aagccgcatt ctatgttgct gtgggggccg ccgggcgtgg gcaagacgac gttggcgcag
     1741 attttggcgc agagtttcaa cgcccagttt ttgcctgttt ccgccgtatt ttccggcgtg
     1801 aaggacatcc gcgaggcaat cgataaagcc gaaatcgctt tgcagcaggg acgcgcgacg
     1861 attttgtttg tcgatgaagt ccaccgcttc aacaaggcgc agcaggacgc gtttttgccg
     1921 catgtcgaaa gcggtttgct aacctttatt ggtgcgacga cggaaaatcc gtcgtttgaa
     1981 gtcaatcccg cgctgttgag ccgcgctcag gtgtatgttt tgcaaccctt gtcttcagac
     2041 gacctgaaaa agctgattgc caaggtattg gctttgcctg aataccaaga gtttacgatt
     2101 gaaacggatg cgcaaaaatt actcgtgaat accgccgacg gtgatgcgcg cagattgttg
     2161 aatttgttgg aacaactttt acgcgccgcc gatacacgtc gtctgaaaaa cttaaccgcc
     2221 gaatttctcg ccgacagtct cggggcgcaa atccgccgtt tcgacaaagg cggcgagagt
     2281 ttctacaacc aaatctccgc cctgcacaaa tccgtgcgcg gttcgcatcc gaacgccgcg
     2341 ctgtattggt tctgccgtat gctcgacggc ggcaccgacc cgcgctacct cgcccgccgc
     2401 atcgtgcgta tcgcttggga agacatcggg cttgccgacc cgcgcgcctt ccaaatcgcc
     2461 aacgatgccg ccgccacctt cgaacgctta ggctcgcccg aaggcgaact ggctttggcg
     2521 caagcggtat tgtatcttgc cgccgccgcg aaatccaacg cgggctacaa ggcatacaac
     2581 caaatgcgcc gtttcgtcaa agaaaacgcc agcgacgaag tacccgtcca cctgcgcaat
     2641 gccccgacca agctgatgaa ggaattgggc tacggacgcg aataccgcta cgcccacgac
     2701 gaaccgaacg cctacgccgc cggcgaaagc tatatgcccg acggcttgga cgaaccggac
     2761 ttctaccaac ccgtcccgcg cgggttggaa atcaaaatcg gcgaaaagct ggcgtggctc
     2821 aaatcgctgg atgaagaggc attaaaggca aaatgaagca atgccgtctg aagcagaaca
     2881 gtatggcaaa gccggtacac cgaaaaggcg tatcgggttt ttcaggtgtt ataatccgtt
     2941 tcaataaatt ttgatgaatc attacgttta tgtcccaaac tcacaacgaa aattcccgcg
     3001 tcaaaatccc cgccgttttg cacctgatgc gcttgggcta cgactacctc tcgctcaaaa
     3061 acgaaaattg ggataaacca accaacatct ttcccaaaat ctttatagaa agcctctgcc
     3121 gcatcaatcc cgatttgtcg ccggacgatg cacgccgttt gctgaccgac atacgcttag
     3181 aactggacaa cgaagacttg gggcaaaagt tttacgaacg ccttaccaat caatccggcg
     3241 gcaagaaact gatcgacttc caaaattttg ataacaacag cttccacgtt gtaaccgaat
     3301 tgccctgtat caacggcgat gaagaattcc gccccgacat taccctgctg gtaaacggca
     3361 tgcctttggt ttttatcgaa gtcaaaaagc ccaacaacaa aggcggaatc ggcgaagagc
     3421 gggagcgcat gggcaaacgt gcgaaaaatt ccaaatttcg ccgtttcatc agcatcaccc
     3481 aattcatgat tttttccaac aacatggaat acgacgacgg cgcaaccgaa ccggcacaag
     3541 gcgcgtttta cgcttcgtct gcttacggta agcccgtgtt taactacttc cgcgaagaac
     3601 ataaattaaa tcttgccgaa ttattgaaca cgctttcaga cggcctcgaa aacaacgtcc
     3661 tgcaagacaa caacctgccc gttatcaaac acagccccga atttatcagc aataaatcgc
     3721 ccgatacgcc gaccaaccgc atcctgactt cgctgctttg ccgcgaacgt ctttcttttc
     3781 ttctgcaaca cgggctgact tacgtcaaag caagtcaagg cttggtgcaa aaacacatca
     3841 tgcggtatcc gcagctattt gccactctcg ccattgaaaa acatttggca aacggcggca
     3901 aaaaaggcgt gatttggcac acccaaggct ctggcaaaac cgcgcttgcc tattacaaca
     3961 cccgctacct gacccactat tatgccaaac agggcatcgt gccgaaattc tatttcatcg
     4021 tggacaggct tgatttattg aaacaggcgc agcgcgaatt tactgcccgc gatttagtcg
     4081 tccataccat cgacagccgc gaagcctttg ctgccgacat caaatccgcc caaactttgc
     4141 acaaccacgc aggcaaagcg gaaatcactg ttgttaatat ccaaaaattc caagacgacc
     4201 ccgatgtcgt cgctcgtaac gactacgacc tcgccattca gcgcgtctat tttctcgacg
     4261 aagtacaccg cagctacaac cccaaaggct cgtttcttgc caacctcaac cagtccgacg
     4321 taaacgccgt caaaatcggg ctgactggca cgccgcttat cggcgtaacc gcaggcaacg
     4381 tcaacacccg cgaactcttc ggcgactaca tccacaaata ctattacaac gcctccattg
     4441 ccgacggcta caccctgcgc ctgatacgcg aagaaatcgg cagccgatac aaggcgcaat
     4501 tacaagaagc actggcgcaa cttgaaatcg aaaaaggcag ctttgaccgc aaagaaatct
     4561 acgcccatcc gcacttcgtc cacccgatgc ttgactacat cttggacgac ttcgccaaat
     4621 tcagaaaaac caaccaagac gacagcctcg gcgctatggt tgtttgcgac agcgcggaac
     4681 aggcacgtca gctttttgag cattttcaga tagcctcaga ccataatttc accgccgcgc
     4741 tgatattgca cgacgtcggc actaaggacg aacgcgacca atgggttaaa gatttcaaag
     4801 cgggcaaaat cgacatcttg tttgtgtaca acatgctttt gaccggcttt gacgcgccgc
     4861 gcctgaaaaa gctctatttg ggcagactga taaaagccca taacctttta caaaccctga
     4921 cccgcgtcaa ccgcacctac aaatcttacc gctacggcta tgttgtcgat tttgccgaca
     4981 tcgagcgcga atttgacaaa accaaccgcg cctattggga cgaactttcc aacgaattgg
     5041 gcgacgaaat cggcagctac agccggcttt tcaaaaccgc cgaagaaatc gaacaggaaa
     5101 ttgcagacat taaaaacgcc ctgtttgatt tcgataccga aaacgccgaa gaattttgca
     5161 gccaaattag ccaaatagca gacaaaaaac agctgctcgc cctaaaaaaa gccctgcaaa
     5221 ccgccaaaaa gctgtacaac gtcctgcgcc tgcaaggcag ccacgaattt ctcgcccatc
     5281 tcgattttga caagctcaat cttttgtacc gcgaaaccgc cgcgcgtttg gagaccttaa
     5341 accttgccga aaaactacag caaggcgaca ccgcccattt gctcaatgaa gcgctggaag
     5401 acgtttactt tcaattcgtt aaaatcggcg aagccgaact caaacttgcc gacgatttga
     5461 aagacatcat gcgcaaagtc cgcgaagggc tggcgggcaa cttcgatcaa gaagaccccg
     5521 aatacatcag cctgcgcgaa gaactggaac gcatcttcaa gaaaaagaac ctcgccgaag
     5581 tcgggcaaga agaaatgcag gcaaacatcg ccatactcga aaccgtctat gccaaaatca
     5641 aagagctgaa ccgccaaaac gacctcttgc ggcacaaata cggcggcgac gccaaatatg
     5701 cccgcatcca caaacgcctg cgcgaaaacc ccgccctcta cggcgataaa ctcaaagtct
     5761 ttaacgcgct gaatggtgta aaaaccgaca cggaccaaaa agtgctggat atggagcaga
     5821 ttttggacaa ccaaaattac tttgaaaaac aaatgcaggg catcgtatta aaacgcttta
     5881 gaacggaaca gcaatttccc gttcaacccg cagacatcca agccatcaac cgcctgttgg
     5941 tgcgggaata tttaaaagaa agtgggcgga tttgaggagt agattttcag gctgccttaa
     6001 ccatttataa atacaggaaa gctatgtaca acaacgacat catcatctac accacggaag
     6061 acggactatc cgaatttacc ctgcgcgagc ttgacggcga actatggctt acccaaaaag
     6121 aaattgccga actctaccaa accagcaaac aaaacatcgg taaacacatc aaagccatat
     6181 ttgcagaaca agaactggac gattcagttg taaactttca gtttacaact gctgcagacg
     6241 gcaagaacta ccgcatgggg ctttatcccc tctccctaat catcgccgtc ggctaccgcg
     6301 tccgctccac acgcggtaca caattccgcc aatgggcaac acggacattg ggcgaataca
     6361 tcagcaaggg cttcgtatta aacgacgacc gcctgaaaaa cccgcctgtc gggacaaacc
     6421 gtgccgaaga tcagtttgaa cagttgctca accgcatcag ggacatccgc agcagcgaac
     6481 ggcggatgta tctgcgtgtg cgcgaaatat tcgccatggc ggcggattac cagcccagct
     6541 tcaaagaaac cacccatttt ttcaaaatca tacaaaacaa acttcattac gcctgtaccc
     6601 gttataccgc ccctgaaatt atttaccaaa gggcagatgc ggggaaaccc aatatggggc
     6661 tgacccattg gcggggcaaa gaaatcgcca aagccgatgt aaccgttgcc aaaaattacc
     6721 tgaacgaggc agaaatcgac gccctcaacc gcatcgtttc catgtggctg gattttgccg
     6781 aagaccaggc aagcatgaaa aaacagatat tcctgcaaga ctgggcggaa aaactggatg
     6841 ccttcctcgc cttcaatgac cgccaagtct tacaaacggc agggacaatc agcaaaacgc
     6901 aggcagacga aaaagcccgc atcgaatacg aaaaatttgc cgccgcacgc agactggaaa
     6961 aagaacgcga gggcgaacgc catattgccg aactgctgaa cctgaaaaaa tagcaggcat
     7021 caatgcaggc tgcttaaaaa agtgcggtcg gattttgaaa tgttttttca gacggccttt
     7081 gaagagtttt tagagataca gttaagatga atagtgcatt aaagaatcat gcaacatata
     7141 taaataacaa agttgataca aatgaaattt cgcttagcca atatatttca acagacaata
     7201 tattgcaaaa taagcaaggt atagaatgtg caacgtcttt accaattcaa ggtggaaaag
     7261 taacttcatt taaaaagggc gatattttat tagccaatat acgaccatat ctaaaaaaaa
     7321 tttggtatgc ccaatttgat ggaggttgtt ctgctgatgt tttagcgatt cgtgctaata
     7381 cagaaacaga tagtcatttc ttattttatg ctttgtttcg tgatgatttt tttatccatg
     7441 caatgaaagg tgcaaagggt acaaagatgc cacggggcga taaaacacaa attatggaat
     7501 ttaaaattcc agtttttgac ctcaaaaccc aacaatccat agccgctgtc ctatctactt
     7561 tggacaaaaa aattgccctg aacaaacaaa tcaacgcgcg cttggaagag atggcgaaaa
     7621 ccctgtacga ctactggttc gtgcagttcg acttccctga cgccaacggc aagccctaca
     7681 aatcctcagg cggcgaaatt gtgtttgatg aaaccttgaa acgggaaatt ccgaaggggt
     7741 ggggaagtat agaattacaa tcttgcttag caaaaatccc aaatactaca aaaatattaa
     7801 ataaagatat taaggatttt ggaaaatatc ctgttgttga ccaaagtcaa gattttattt
     7861 gtggatttac caatgatgaa aaatctatat taaacccgca agatgctcat attatttttg
     7921 gtgatcatac aagaattgtt aagttagtta attttcaata tgcgcgggga gcagatggta
     7981 cacaagtaat attaagcaat aatgaacgaa tgccaaacta tttgttttac caaatcataa
     8041 atcaaataga tttatcaagt tatggatatg ctagacattt caaattctta aaagaattta
     8101 aaataatttt acctagtaaa gatatttctc aaaaatataa tgaaatagca aatactttct
     8161 ttgttaaagt tcgaaataat ataaaacaaa accaccaact aacccaactc cgcgatttcc
     8221 tacttcccat gctgatgaac gggcaggttt ctgtggcgga ataagccaaa ttcaagaatc
     8281 agacctacaa aataaaacaa acgagaaaaa aatgaccgaa caacacttta ccgaacaaac
     8341 caaatcccta atcgacagct taaaaaccat ttgcgccaac tatggtttgg gcaatgacgg
     8401 caacgagttc aaaatcatca gtcaggcatt tttgtataaa ttcctaaacg acaaatacga
     8461 tttcgaagtt aaacaaattc gcaaagaaaa tcccgacgag ccaatcgaat tcatcaatat
     8521 ggatattgag ggaaaaacag ccgttttgaa gcctgaacat tccatcaagt atttatcaga
     8581 gcgacaaaac ggcgcggact ttgccaaact ctttgacgat acgctgaccg atattgccgc
     8641 acataatgcg gaactgtttt cggtaaaaac cgaaggcggg gcgaaaatcg tgttgttcga
     8701 gcgtatcagt caatatatta cggatgaagg ccgccgcgac gatttttgcc gtgcgctgat
     8761 ttccaaactg gcgggtttta gtttcgaggc gatttttgcc caaaaatttg atttttttgc
     8821 cacgattttt gaatacctga ttaaagacta caacagtaat tcgggcggca aatacgccga
     8881 atactacacg ccgcacgcag tagcgcggat tatggcggat attttggtgc cggaggaagt
     8941 gcgcgggcaa atccgcagcg tggacgttta cgatccgtcg gccggttcgg gaacgctgtt
     9001 gatgaatgtc gcccatgcca tcggcgaaga taaatgcatg atttatacgc aggatatttc
     9061 gcaaaaatcg tccaatctgc tacggctgaa tctgattttg aataatctgg tgcattcttt
     9121 gaataatgtg gtgcagggca atacgatttt gtcgcccgcc cataaagacg cttcaggccg
     9181 tctgaaaaaa tttgatttca tcgtttcgaa tccgccgttt aaattggatt tcagcgattt
     9241 ccgcgaccaa ctagaaggcg aggaaaaccg cgaacgcttt tttgccggta tcccgaaaat
     9301 caaggcgaaa gatacggata agatggaaat ctaccagctt tttatccagc atatcttgtt
     9361 ttccctgaaa gaaaacggca aagcggcgat tgtgttgccg acgggcttta tcactgccca
     9421 atcaggcatc gacaaaaaaa tccgcgaaca tctggtagaa aacaaaatgc tcgccggtgt
     9481 ggtttccatg ccttccaata ttttcgccac cacgggaacc aacgtttcca tcctgtttat
     9541 cgataaagcc aataaagata aagtggtatt aatcgacgca tcgggcttgg gcgaaaaaat
     9601 caaagacggc aaaaaccaaa aaaccgtact ttctcgcgaa gaagaacaaa aaatctgcaa
     9661 taccttcacg cacaaacagg cagtggaaga tttcagtgtg gtaatcggct acgatgaaat
     9721 caaagcgaag aatcacagct tgtcggcggg gcagtatttc gaggtaaaga ttgattatgt
     9781 ggatatttcc gccgacgaat ttgcgcaaaa aatggcggga ttttcagcgg atttggataa
     9841 acttttcgcc gaatctgccg aattggaaaa agagattaag gatagattgg cgatgttgaa
     9901 attcaattca taaataccga tgaatgaaac cggtcaaact taaaatgccg tctgaaagcc
     9961 tttcagacgg cattttgttt ggtttggaaa atacttatgc cgttctttcc ctgaaagaga
    10021 gaatccaaaa aaccaaagcc acaggaattt atcggaaatg acaaaaaccc gacgaaccgg
    10081 attcccgcct gcgcgggaat gacgaattag aagttacccg aaatttgcaa aaacaaaatc
    10141 caacccaaca gaccgggttt tcgtttgcac ggaaataatg caataaataa agcaaatata
    10201 aagtatttga atttactata ttatttttcc gcttcttcaa agccgacgac ttccaaaccg
    10261 aagccggtca ggccggtgaa agatgagggc tgcccgagga cgcgcagttt tttgacgttg
    10321 aggccggcga ggatttgtgc gccgatgccg tagcttttgc tgtcccattt gtaggcttgg
    10381 tttgcgcctt tgggcagggt gcggtcgagc agggatgcgc cgtcttcggt gcggtgcaga
    10441 aggatgacga cgccgctttc tgcctgttgg acgcgctcga gtgctttggg cagcgaccat
    10501 gaatgacgcg ggttagcttg gatgaagtcc atgacgctga agggttcgtg gacgcggacg
    10561 agggtttcgg tgtcggcgga gggcgtgcct ttgacgaggg cgaggtgggt ttcgccggag
    10621 agtttgtcga cgtaaacgtg ttgttggaac tcgccccacg gggtttgtac aggcgcattg
    10681 cccatgtctt caagcaggct ttcggtacgg ctgcggtatt cgatgaggtc ggcaatcgtg
    10741 ccgattttga gcttgtgttc ttcggcgaat ttcatcagtt cgggcatacg cgccatcgtg
    10801 ccgtcgtcgt tgatgatttc gcaaataacg gaggcaggaa tcagcccgtt catttgcgct
    10861 aggtcgacgc cggcttcggt gtgtccggcg cggacgagta cgccgccttt ttgggcgcga
    10921 agcggaaaga tatgaccggg ttggacgatg tcttcgggtt tagcggtcgg ggaaacggcg
    10981 gtttgaatag tcagggcgcg gtcggcggcg gaaatgccgg tggtaatgcc gtgtgcggct
    11041 tcgatggaga cagtaaagtt ggtgccgtat tgcgcgccgt ttttttgggt catcatcggc
    11101 agcccgagtt tttcgaccat ttcgccgtcc atcggcaggc agaccaagcc gcgcgcgtgt
    11161 ttgatcatga agttgatggc ttcgggcgtg acgaattgcg ccgccatcag caggtcgcct
    11221 tcgttttctc ggtcttcggc atcggtgatg atgaccattt tgccggcttt gatgtcggct
    11281 aggatttcgg ggatggggga gatatgggac atggtgtgtt ttcctgtgtg ttcggacggg
    11341 cgggcggtgt gttcggtatc gagccaaaag gcgggcattt ttgccgcctg ttcgatttta
    11401 cgggcttttt tctcgccgaa ggatttgttt ttcaaaaggg cggagagttc gcctgtgttg
    11461 agggcaacgg cttcagcaaa acgagtttgc aaaccgccgt agtatttttc tatccactgc
    11521 cgcaggttgt ggcggcgcag accggctgtg tcggtcatcg tttcatcctt tgtagaaatc
    11581 ggatgtagcg gattgtaatc gcgtatttac caaaaagcaa atctgttttt ttcgccaaac
    11641 cgaatatttg ctttttggta aacagatgcc gtctgaagcc ggattcgtgg cttcagacgg
    11701 cattgccgca atccccgccg gcggggcggc tgtttcttcc cgcccgcttc cgattcgggg
    11761 tttgccggaa gcttattccg aaaaaaattt ctggtaaacg gccttgattg ccgggtacag
    11821 cataggcggt acgagcaggc gcaatatcgg caatcctatg atgaatgcgg tcttcccgat
    11881 aaaaaccgga cggattgcct tcgcgccgaa tatctttctg catttttctt taaatgaaaa
    11941 atatttccgg tagatgagga taatgttttt ggaaaaataa agccttacca atatagacct
    12001 cagatagccg atattgtcga agacaaattt ttgcaaaccg ccgtagatgg gggaattttt
    12061 gttttcgact aaaaaatcca caccccggtc gagatgcgtc agcacatcgc tgaaattttt
    12121 gtatttgatg ttgtggctga tagagccgcc gagaacgcgg tactggtaaa aagggttatc
    12181 gtaaaaggca aaagtcttga taaaacgcgc caattgcaaa ctgtacggga aatcctcgtg
    12241 aatgtatcct tttgggaaaa acagattgtt tttgatgatt atttcccgcc tgacgatttt
    12301 tgtccacgcg ttggcgatat agtaccgccc ctccaccaac gtttcaaaat ggcggacaaa
    12361 atcattatcc gaaaagtccg cccctttggg gatgacgtgg taattgaggg acgagggatg
    12421 caggatcaaa tcaacctttt tgtctgcaag ttgttgtaaa tcaaagagaa tccccccccc
    12481 ccgcgttttt tgaacggttg gtatcggccc aatagtcgtc gctgtccaaa aagatcaggt
    12541 aatcgccttt tgccgctcgg ataccggcgt tgcgggcatc cgacagcccg ccgttttctt
    12601 gatgaatcac ttttatatgc ggatatttgc ctgcatattc gtcgcaaatc ttcccgcagc
    12661 catccggcga accgtcatcg accaaaatca tttcataatc ggcaaaattt tcggcaagca
    12721 cggaatccac gcagcagcgg aggtattttt ccacattgta aatagggacg atgatggaga
    12781 aaatcataaa tatcaatcca ttatattaag atgtttgcgc gtatgcctca aacccgcacc
    12841 cgcaatgcgt ttgcatccgc accctgtaac tttataaaac ttattgcttt atcaaggtat
    12901 ggaaacctgt ttcccgaaag gcggcgcagg atgcccgttc cctgcatctt tgccttattc
    12961 cgacatcaga tgccccagct tgtctgcttt ggtttggagg tagcgtttgt tttcaaggtt
    13021 ttccccgacg tgcaggggga tgcgttcgac cacgttaatc cccgcatctt tcagggtttg
    13081 gattttttcg gggttgttgg tcagcagttt gaccgagcgg atgcccagat attcgtagat
    13141 agattgcgcc aaacggaaat cgcgggcatc gacgggcagc ccgagtgcca aattggcttc
    13201 aaccgtatcc ataccttggt cttgcagatg ataggcgcgg attttgttaa tcagcccgat
    13261 gccgcgtcct tcctgacgca gatagacgat gatgccgcgc ccctctgtct gtacggccct
    13321 catggccgct tcaagttgag gtccgcagtc gcattttctc gagaacagcg catcgcccgt
    13381 caggcattcg gagtggatgc gcgtcagaac cggattgccg tctgaaaaat caccgacggt
    13441 cagcgcgacg tgttcctgcc catttgcctc ttcaaagccg tgcatcgtaa atacgcccca
    13501 ttcggtcggc aggcggcagg atgccacatg gttcaacatt tcagacatct tcactcccat
    13561 cttttccggc agacataccc aacagcagtt tcaaaggttc ggacaaagcc aaggcaagag
    13621 ccacccattc cacctgtgct gccgtacccg cacactctgc gcattcaaat tccacatgaa
    13681 ccacgcccaa cacaccgcca ctttccgtgc agaccggaat ggaaatttgc gccgccgaag
    13741 catgattgcg ttctcccgaa agctctccca aatccaacca acgacgcaca tccgaggcaa
    13801 tattcatcca gccgctttgc gccgaacggc aagccagcga aaccttgcct gccgcttcat
    13861 ccaaatccca caggttttcc aaaacctcgc cctgccggga caggcaaacc agccgaaagg
    13921 cggcattttc agacggcatc agggcataaa cggctgcggc gcgcacgcct ttacagcgtg
    13981 aaaacaccga atccaaagcc atgaaaagcc gtttcagacc ttcctcattc ccgctgtttt
    14041 gctccaaata atcggcaagc ctccaatcct catgctcaag ccacaaaacc gaacgctcaa
    14101 tcgaagccgt ccccatatcc attaccgtct gcgccgtcag atacgccgcc cgaacctcgt
    14161 caagcggcag cttcaaaccc tgagtcagca gaaaatcctt aatcaaagca gcaggcatac
    14221 ccaaattccg tcaataaaaa caaaaaaccg cccgattccg atgtcaggcg gacgaaaaac
    14281 agattttacc gccccaaacc ccaagccatc aacagccccg ccgaaaacct tgcacaaaaa
    14341 aacaaaatct gtataattgt ccaattccca tgcggacgtg gcgaaattgg tagacgcacc
    14401 agatttaggt tctggcgccg agaggtgtga gagttcgagt ctctccgtcc gcaccaataa
    14461 tgataaataa atcatatagt taatgcttat cgggacaaat tagtgccaca aaacataagt
    14521 cgtctgcttt tcagcgggcg gcttttttat ctgcctatat gtttcaacac gcagaacggt
    14581 acataaagtg ccgccatttg ttccgcaccg aatcggaaac gggtgcttcc gcccgcaatc
    14641 agattcactg cccctaaatg ccggggtttc aaccggaaag aagaaaatat tgattttgat
    14701 gatggcatca aaaaacccct tccgcccgtc tgccaaaagg aatggttaag aaaagcaatg
    14761 cgtcttttga tggaacgcga tatataagga attgagggga agggcaggcg ggtttctgcc
    14821 cgttaatcct gcggacggtc ccgccaaacc cgacacgcgc gggcatatgg cggattgttt
    14881 acaatattgt ctaaaaatct gaacctgcaa acgtgaaata aggtagaata cgccccgtta
    14941 ttttaccgtc caatctccat ttaccaagga aaaacgatga gtacttcatt gagttaccgc
    15001 gatgcaggtg tcgatatcga cgcaggcgac caactggtcg aaaacatcaa accgtttgcc
    15061 aaacgcacga tgcgcccgga agtattgggg gatttgggcg gcttcggcgc gttggtcgaa
    15121 atcggcaaga aatataaaaa tcccgtattg gtcagcggta cggacggcgt gggtaccaag
    15181 ctcaaacttg cctttgattg ggataaacac gatacggtgg gcatcgacct tgttgcaatg
    15241 agtgtcaacg atattttggt tcaaggggct gagcccttgt ttttcttgga ctattttgcc
    15301 tgcggtaaat tggatgttcc gcgcgcgacc gatgttatta aaggcattgc ccaaggttgc
    15361 gaagaatccg gttgcgccct gattggcggg gaaactgccg aaatgccggg tatgtatccc
    15421 gtcggagaat acgatttggc gggttttgcc gtcggcgtgg tggaaaaaga gaatgttatt
    15481 accggccgca gcatcggcgt aggcgatgtg gtattgggtt tggcttccaa cggcgcacat
    15541 tcaaacggct attcccttat ccgtaaaatc atcgaacgcg acaatcccga tctggatgcc
    15601 gagtttgata atggcaaaac cttgcgggag gctgttattg cgccgacccg tctgtatgtg
    15661 aaaccgattc ttgccgcttt ggaaaaattt accattaaag gtatggcaca cattaccggc
    15721 ggcggcatta ccgaaaacgt gccgcgcgtg ttgcctgaaa acacggttgc acaaatcgat
    15781 gctaaatcgt gggaattgcc caagctcttc caatggcttc aaaaggcggg caatgtggaa
    15841 acccaagaaa tgtaccgaac ctttaactgc ggcatcggca tggtcgttat tgttgctgcc
    15901 gaagatgccg atgcggttca gggtctcttg ggtgaacaag gtgaaacagt ttaccgttta
    15961 ggtttgatcc gtgagcgtca gggagacgag catcaaaccc aggttgcctg attgcttcta
    16021 tagcgaaatg ccgtctcaaa catgggtttg gacggcattt ttatag
//
LOCUS       DAXHSJ010000031        15972 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000031 DAXHSJ010000000
VERSION     DAXHSJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15972
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.031"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..77)
                     /locus_tag="ACFM8U_000725"
     CDS             complement(<1..77)
                     /locus_tag="ACFM8U_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6334000.1"
                     /translation="MKPVVWAALLLCACTSNFGDREHQFL"
     gene            280..1608
                     /gene="miaB"
                     /locus_tag="ACFM8U_000726"
     CDS             280..1608
                     /gene="miaB"
                     /locus_tag="ACFM8U_000726"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC6334001.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIVKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYCGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPNMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1693..3606)
                     /gene="dxs"
                     /locus_tag="ACFM8U_000727"
     CDS             complement(1693..3606)
                     /gene="dxs"
                     /locus_tag="ACFM8U_000727"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC6334002.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYNTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3691..4608)
                     /gene="xerC"
                     /locus_tag="ACFM8U_000728"
     CDS             complement(3691..4608)
                     /gene="xerC"
                     /locus_tag="ACFM8U_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC6334003.1"
                     /translation="MGLDGLAAYLDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4792..5856
                     /gene="fba"
                     /locus_tag="ACFM8U_000729"
     CDS             4792..5856
                     /gene="fba"
                     /locus_tag="ACFM8U_000729"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC6334004.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVDAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            5876..6838
                     /locus_tag="ACFM8U_000730"
     CDS             5876..6838
                     /locus_tag="ACFM8U_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC6334005.1"
                     /translation="MPSEPPFGRHLIFASLTCLIDAVYKKRYHNQDVFILSILRMTRS
                     KPVNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLDHKDKGLQSLTLD
                     QSVRKKEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQVYKQSYSALTALQTEQEQDSEHSGKMVAKRRFRIGDIAGEHTSFDKLPKGSSATYR
                     GTAFGSDDAGGKLTYTIDFAAKQGYGKIEHLKSPELNVDLAAANIEQDEKHHAVISGS
                     VLYNQDEKGSYSLGIFGEKAQEVAGSAEVKTANGIRHIGLAAKQ"
     gene            6984..7661
                     /gene="tsaB"
                     /locus_tag="ACFM8U_000731"
     CDS             6984..7661
                     /gene="tsaB"
                     /locus_tag="ACFM8U_000731"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC6334006.1"
                     /translation="MQADFNRPILAIDTGTSHLSLALRADGETRLFHQEVGSRQSELI
                     LPEIRTLFRDAGITAADLGAVVYAQGPGAFTGLRIGIGVAQGLATPFDTPLIGVPSLD
                     AAASLPPPQSCILAATDARMGEVFYAWFDTLNCRRLSDYQVGRAADIRLPEGCTFSDG
                     IGSAFALEEAPPFSGRPDMPTAADFLALAAKGGYPAVHAADAGLLYVRNKIALTAKEQ
                     AERRARP"
     gene            7658..8098
                     /gene="rimI"
                     /locus_tag="ACFM8U_000732"
     CDS             7658..8098
                     /gene="rimI"
                     /locus_tag="ACFM8U_000732"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC6334007.1"
                     /translation="MNIRRAVYADCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRLAAFIVWQNLPDESELHLIATAPAYRRRGVASALLEYWFAHLPEGTQRLLLE
                     VRAGNIGAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8092..8832
                     /locus_tag="ACFM8U_000733"
     CDS             8092..8832
                     /locus_tag="ACFM8U_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6334008.1"
                     /translation="MLSARYLHLHEALGLGPMWLKRGAVVLPSATLPGSPTQIRLQKQ
                     TVLSIPQRPSEQHTGQARLKTMKVLETTAVPTRKPMPETETPLSGVSDGIAPVSAASG
                     ITKLAVVSLCPPTEDMVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNP
                     MPSEQAIANALGQITRELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPA
                     RLLRQPELKARNWQVLKQLKRALQAGGS"
     gene            8894..9535
                     /gene="pyrE"
                     /locus_tag="ACFM8U_000734"
     CDS             8894..9535
                     /gene="pyrE"
                     /locus_tag="ACFM8U_000734"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC6334009.1"
                     /translation="MTDFRQDFLKFSLVQNVLKFGEFTTKAGRQSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIQFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9609..10043
                     /locus_tag="ACFM8U_000735"
     CDS             9609..10043
                     /locus_tag="ACFM8U_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC6334010.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGMFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10040..11350
                     /gene="argA"
                     /locus_tag="ACFM8U_000736"
     CDS             10040..11350
                     /gene="argA"
                     /locus_tag="ACFM8U_000736"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC6334011.1"
                     /translation="MIVPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYGGKTLHLDMLQTAASAAVSLQAEKLVYLTLSDGIARPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEAELPEARRKAYRNNGRNSHILVRRLHR"
     gene            11446..13461
                     /locus_tag="ACFM8U_000737"
     CDS             11446..13461
                     /locus_tag="ACFM8U_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC6334012.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGIEVRQFWTTSADGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSLPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGASWTDEYGNPQKYEVCKRQLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(13575..14531)
                     /locus_tag="ACFM8U_000738"
     CDS             complement(13575..14531)
                     /locus_tag="ACFM8U_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6334013.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            14767..15732
                     /locus_tag="ACFM8U_000739"
     CDS             14767..15732
                     /locus_tag="ACFM8U_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6334014.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAGKE"
BASE COUNT         3543 a         4795 c         4401 g         3233 t
ORIGIN      
        1 aggaactgat gttccctgtc gccaaaattg ctggtacacg cacacagcag caatgccgcc
       61 catacaaccg gtttcataca catctcccat taaagccaaa cattatacag ccgtccaaac
      121 cgattaaatt catattttaa aacaatatcc tgcctccaaa acccacatcg tgctataatc
      181 cacaccgatt ttcagacggc atcgtcatgc cgtctgaaat tttttcattc caacaacaat
      241 cagccccgcg attacggctg cctgagaaag acacaaacca tgaaaaaagt atttatccgc
      301 accttcggct gccagatgaa cgaatacgac agcgacaaaa tgctcgccgt cctcgccgaa
      361 gaacacggcg gcatcgaaca ggttacccaa gccgacgaag ccgacatcat cttgttcaac
      421 acctgctccg tgcgcgaaaa agcgcaagaa aaagtcttct ccgatttggg gcgcgtgcgt
      481 ccgctcaaag aaaaaaaccc cggcctcatc atcggcgttg ccggttgcgt cgcctcgcaa
      541 gaaggcgaaa acatcgtcaa acgcgcgccg tatgtggacg tggttttcgg cccgcaaacg
      601 ctgcaccgcc tgcccaaaat gattgtggac aaagaaacca gcgggctgtc gcaagtcgat
      661 atttcctttc ccgaaatcga aaaattcgac cacctgccgc ccgcccgcgt cgaaggcggc
      721 gcggcatttg tatcgattat ggaaggctgt tccaaatact gctccttctg cgtcgtcccc
      781 tacacgcgcg gcgaagaatt ctcccgcccg ctcaacgacg tattgacaga aatcgccaac
      841 cttgcccagc aaggcgtgaa agaaatcaac ctcttgggac aaaacgtcaa cgcctattgc
      901 ggcgaaatgg acgacggcga aatctgcgac ttcgccaccc tgctgcgcat cgtccacgaa
      961 atccccggca tcgaacgtat gcgcttcacc accagccacc cgcgcgagtt taccgactcg
     1021 attatcgagt gctaccgcga cctgcccaaa ttggtttccc acctgcacct gccgattcaa
     1081 agcggttccg accgcgtatt gagcgcgatg aaacgcggct acaccgcttt ggaatacaaa
     1141 tccatcatcc gcaaactgcg tgccatccgt cctgatttat gcctgagcag cgatttcatc
     1201 gtcggcttcc ccggcgagac cgaacgcgag ttcgagcaaa ccttgaaact ggtgaaagac
     1261 attgccttcg acttgagctt cgtgtttatt tacagtccgc gccccggcac gcctgccgcc
     1321 aacctgcacg acgacacgcc gcacgaagaa aaagtgcgcc gcctcgaagc cttgaacgaa
     1381 gtcatcgaag ccgaaaccgc gcgcatcaac caaacgatga tcggcacggt tcaacgctgc
     1441 ctggtcgaag gcatctccaa aaaagacccc gaccagctcc aagcccgcac cgccaacaac
     1501 cgcgtcgtca actttaccgg cacgcccaac atgattaacc aaatgatcga tttggaaatc
     1561 accgaggcct acaccttctc cctgcgcggc aaagttgtcg aagcctaaac ccttacgccg
     1621 aaaaaatgcc gtctgaagcg tttcagacgg cattttgcct tgtatcggca gacgatggcg
     1681 cggccaggcg gcttaatttg ccgcatcccg atccgacaac cacgcgcgca cacgccgttc
     1741 caccgcttcg gcactcaagc ccaaatcgtc taaaagtttt ttcggatcgc cgtgtccggt
     1801 tacggtatcg gcaacgccca aaagcaaaac gggtttgcag atgccgtgtt tcgccaatac
     1861 ttccagcacc gcgccgcctg cgccgccctg ttcggcgttt tcttcaaggg taacgatgcg
     1921 gtcgtggctt cgggcaaggc ggacaatcaa ctcttcgtct atcggtttga cgaagcgcat
     1981 atcggcgacg gtggcgttca gtttttcggc aaccgccaat gcgggggcga ccatactgcc
     2041 gaaggcaatg aatgcggttt tctcaccttc gcggcggata atgcccttgc cgatttccac
     2101 ggtttccatg ccgtctgaaa ccggcgcgcc cgtacccgtg ccgcgcggat agcggacggc
     2161 ggcgggcgcg tctgcctgat agcaggtcga aagcaacagg cggcattcgt tttcatcgct
     2221 cggcgcggcg acaatcatgt tcggcacgca gcgcaaaaag ctcaaatcgt acagaccggc
     2281 atgggtcggg ccgtccgcgc cgacgatgcc cgcgcggtcg acggcaaaca aaacgggtag
     2341 gttttgcagg gcgatgtcgt gcaccagttg gtcgtaggcg cgttgtaaaa aggtggaata
     2401 aatcgccacg acgggcttca tgccttcgca cgccaaaccg ccggcaaagg taacggcgtg
     2461 ctgctcggca atgccgacat cgaaataacg gtcggggaat cgttgttcaa actcaaccaa
     2521 gccgctgccc tcgcgcatcg cgggcgtgat ggcgatcaaa cgctcgtctg ccgccgcccg
     2581 gtcgcacagc catttgccga acacttgggt ataggtcggt ttggcgacgg gcttgggttc
     2641 tttttcagac ggcatttgcg ccgcgccttc tttaggcagc ttggcgacgg cgtggtattt
     2701 gacggggtcg ttttcggcga gtttgtagcc gttgcctttt ttggtgatga cgtgcagaag
     2761 ctgagggcct ttgcggctgc gcaagtcttt caatacgtcc accagatttt cgacgttgtg
     2821 tccgtccacg gggccggtgt agcggaagcc gaagttttca aacaaagaca gcgactgttt
     2881 ggcgtgttcg gcttcttcgg caagggtttt gattttgtgt tcgacttttt gggcaaactc
     2941 catcgcgccg ggtattttgt ctaatacctt gcccgtttgc gctttgacgg tactcaacag
     3001 gccgtgcata tcgcgcacga cgttgctggc aaggtatttc ggcagcgcgc cgacgttggg
     3061 ggaaatcgac atttcgttgt cgttgaggac gaccagcaaa tccacatcca tatcgcctgc
     3121 gcaattcaag gcttcaaacg cctgccccgc cgtcatcgcg ccgtcgccga tgatggcgac
     3181 gctgcggcgg tcgctgccca agagtttgtc tgccgccgcc atgcccaacg ccgcgccgat
     3241 ggatgtggaa gaatgcccta cgccgaacgc gtcgtattcc gactcgctgc gttttgggaa
     3301 gccagctaaa ccgccgtatt ggcgcatggt gtgcatctgg tttttgcgtc cggtaaggat
     3361 tttgtgcgga tagctttggt gtccgacatc ccacaccagc ttgtcttcgg gcgtgttgta
     3421 cacatagtgc agggcgatgg tcagctcgac cgcacctaag ttgctggcga aatgaccgcc
     3481 ggtttgaccg acggattcca gcagaaaggc gcgcaactcg ccggcaaggc gcggcagctg
     3541 ttttttgtcc agacggcgca aatcttgcgg gctgtcaatc aggtcgagta gggggcttgg
     3601 gttcatggtg tgtctttttt atgtgtcgtc cgggtgcaac ggccaattat atatcaagag
     3661 tgtgcggctg acggctgatt ttgccgtatg tcattcgtcc tgccgcttgg cgcgcgggtg
     3721 ggcttcgtca tacaggcggg cgatgtggtc gaaatcgagc ttggtataaa tctgcgtggt
     3781 cgaaaggctg ctgtgcccga gcagctcctg caccgccctg atgtcgcgcg aagcctgcaa
     3841 taggtgtccg gcgtagctgt ggcgcatcat atgcggcgaa acgtgcctgc cgtcgccgtt
     3901 ttgcgccgcc cattgcgcca aacgtttttg gatttggcgt tggctcaggc gcgtgccgtt
     3961 cctgccggta aacagggctt tgccgtccga tgccgtctga cgcagcggca gatagttttt
     4021 cagggcttcc acgcttttgc cggtcagcgg cacctgccgc tgcttgcgcc ctttgccggt
     4081 aacgtgtacc cacgcctcgt ccaaatagac atcatctgca ttcaagccgt gtatctcgct
     4141 cacgcgcaaa ccgctgccgt acatcagttc gaacagagcg tggtcgcgca ccgccagcgg
     4201 gtcgccgccg tccacgggca aatccagcat ccggttcagc cattcctgcg gcagggcttt
     4261 gggtacgcgc tcgggctgct tcggcggttt gatgtcggcg gtcgggtcgg tatgcagcag
     4321 accgcgttcc accagccaaa cacaatactg ccgccaagac gacagcttgc gcgccagcgt
     4381 ccgcgcattc aaaccgcgct gcgacagccg ccgcaacgcc gccgtaaaat cgcggcgcga
     4441 caagtcctgc ggcacgccgc cttcagcttc agacggcatt tgtgccaaca gcgcaaacag
     4501 ttcttccaaa tcgcgccggt atgcagcaac cgtatgctcc gatttaccct cgcgcacgat
     4561 attttccaaa taagcgtcca agtatgccgc aagtccgtcc aaacccattc ccacacctaa
     4621 aataacatta gaaacattat cataaatcgg aatatccgaa tcccgaaacg tcaaaacccg
     4681 acaaacctgc atactggcat cgttaatata aaatcaatga gccgtttatg gttttttgcc
     4741 gtaaaaaaca ttataatccg ccttatttac ctattgccca aggagacaca aatggcactc
     4801 gtatccatgc gccaactgct tgatcatgct gctgaaaaca gctacggcct gccggcgttc
     4861 aacgtcaaca acctcgaaca gatgcgcgcc atcatggagg ctgcagacca agtcgacgcc
     4921 cccgtcatcg tacaggcgag tgccggtgcg cgcaaatatg cgggtgcgcc gtttttacgc
     4981 cacctgattt tggcggctgt cgaagaattt ccgcacatcc ccgtcgtcat gcaccaagac
     5041 cacggcgcat cgcccgacgt gtgccaacgc tccatccaac tgggcttctc gtccgtgatg
     5101 atggacggct cgctgctcga agacggcaaa accccttctt cttacgaata caacgtcaac
     5161 gccacacgta ccgtcgttaa tttctcccac gcttgcggcg tatccgttga aggcgaaatc
     5221 ggcgtattgg gcaacctcga aaccggcgaa gcaggcgaag aagacggtgt aggcgcagtg
     5281 ggcaaacttt cccacgacca aatgctgacc agcgtcgaag atgccgtgcg cttcgtcaaa
     5341 gacaccggcg tggatgcact cgccatcgcc gtcggcacca gccacggcgc atacaaattc
     5401 acccgtccgc ccacaggcga cgtattgcgt atcgaccgca tcaaagaaat ccaccaagcc
     5461 ctgcccaata cacacatcgt gatgcacggc tccagctccg ttccgcaaga atggctgaaa
     5521 gtcatcaacg aatacggcgg caatatcggc gaaacctacg gcgtgccggt tgaagaaatc
     5581 gtcgaaggca tcaaacacgg cgtgcgcaaa gtcaacatcg ataccgactt gcgccttgct
     5641 tccaccggcg cggtacgccg ctaccttgcc gaaaatccgt ccgactttga cccgcgcaaa
     5701 tacctgagca aaaccattga ggccatgaag caaatctgcc tcgaccgtta tcttgcgttc
     5761 ggctgcgaag gtcaggcagg taaaatcaaa cctgtttcgt tggaaaaaat ggcaagccgt
     5821 tatgccaagg gcgaattgaa ccaaatcgtc aaataacagg ttgactgtaa acaaaatacc
     5881 gtctgaaccg ccgttcggac ggcatttgat ttttgcttct ttgacctgcc tcattgatgc
     5941 ggtatacaaa aaaagatacc ataaccaaga tgtttttata ttatctattc tgcgtatgac
     6001 taggagtaaa cctgtgaacc gaactgcttt ctgctgcctt tctctgaccg ccgccctgat
     6061 tctgaccgcc tgcagcagcg gagggggcgg tgtcgccgcc gacatcggtg cggggcttgc
     6121 agatgcacta accgcaccac tcgaccataa agacaaaggt ttgcagtctt tgacgctgga
     6181 tcagtccgtc aggaaaaaag agaaactgaa gctggcggca caaggtgcgg aaaaaactta
     6241 tggaaacggc gacagcctca atacgggcaa attgaagaac gacaaggtca gccgtttcga
     6301 ctttatccgt caaatcgaag tggacgggca gctcattacc ttggagagcg gagagttcca
     6361 agtgtacaaa caaagctatt ccgccttaac tgcccttcag accgagcaag aacaagattc
     6421 ggagcattcc gggaagatgg ttgcgaaacg ccggttcaga atcggcgaca tagcgggcga
     6481 acatacatct tttgacaagc ttcccaaggg cagcagtgcg acatatcgcg ggacggcgtt
     6541 cggttcagac gatgctggcg gaaaactgac ctataccata gatttcgccg ccaagcaggg
     6601 atacggcaaa atcgaacatt tgaaatcgcc agaactcaat gtcgacctgg ccgccgccaa
     6661 tatcgagcag gatgaaaaac accatgccgt catcagcggc tctgttcttt acaaccaaga
     6721 cgagaaaggc agttactccc tcggtatctt tggcgaaaaa gcccaagaag ttgccggcag
     6781 cgcggaagtt aaaaccgcaa acggcatacg ccatatcggc cttgccgcga agcaataacc
     6841 attgtggaaa tgccgtccga acacgatatt taacgttcgg acggcatttt gtattgtacc
     6901 gtccgacggc atgtccaagg ggggagatcg ctattttcag gccaaccgct atataatgcc
     6961 gtctgaacca acgagagaat gccatgcaag ctgattttaa ccgtcccatc ctggccatcg
     7021 ataccggtac ttcccatttg tcgctcgcgc tgcgtgccga cggcgaaacc cgtctgttcc
     7081 atcaggaagt cggcagccgc cagtccgaac tgattctgcc ggaaatccgc accctgttcc
     7141 gtgatgcggg cattaccgcc gccgatttgg gtgcggtcgt gtacgcacag ggtcccggcg
     7201 cgtttaccgg actgcgtatc ggcatcggtg tggctcaggg tttggcaacg ccgtttgata
     7261 cccctttaat cggcgtgccc tcgctcgatg ccgctgcctc gctgccgccg cctcaaagct
     7321 gcatccttgc cgctacggac gctcgtatgg gcgaagtgtt ttatgcatgg ttcgatacgc
     7381 tgaactgccg ccgtttgagc gattatcagg tcgggcgggc ggcagacatc cggctgccgg
     7441 aggggtgcac cttttcagac ggcataggca gcgcgttcgc gttggaagag gccccgccgt
     7501 tttcaggcag gccggatatg ccgactgccg ccgactttct cgcattggca gccaagggcg
     7561 gctatcctgc cgtccatgcc gcagacgccg gtttgctcta cgtccgcaac aaaatcgccc
     7621 tgactgccaa agaacaggca gaacggagag cgcgcccgtg aacatccgcc gtgccgttta
     7681 tgccgattgc gcggcactgg ccgcactcga tgccgtctgc aacccgtccg catggacgca
     7741 acgccaattc gaatccgcac ttgtttcgcc gtccgagcag gttttccttg cggaaaaaga
     7801 cggcaggttg gccgccttta tcgtttggca gaacctgccc gacgaatccg aactgcacct
     7861 gattgccacc gcccctgcat accgccgccg gggtgtcgcg tccgccctgc tcgaatactg
     7921 gttcgcacac ctgcccgaag gcacgcaacg cctgctgctt gaagtccgcg cgggcaatat
     7981 cggcgcgcag gcgctgtacg cggcacacgg ctttaccgat gcgggcagac ggaaaaacta
     8041 ttaccgtaca gccgacggta aaaccgaaga tgccgtctta atggagaaaa tatgttaagc
     8101 gcgcgctacc tccacctgca cgaagccttg ggtttgggcc cgatgtggtt gaaacgggga
     8161 gccgtcgtcc tgccgtccgc aacattgccg ggaagcccga cacaaatccg cctgcaaaag
     8221 caaaccgtcc tcagcattcc gcagcgtccg tccgaacagc ataccggtca ggcgcggctc
     8281 aaaaccatga aagtgttgga aacaaccgcc gtacctacgc gcaaacccat gcctgaaacc
     8341 gaaacgcctc tgtccggcgt ttcagacggc atcgcccccg tttccgccgc ttctggcata
     8401 accaaacttg ccgtcgtcag cctgtgtccg ccgaccgagg atatggttta cgggcaactg
     8461 ttccacggca aagcgggtgt cctgcttgac aatatactca aagccgtagg attggatgcc
     8521 gcttatgtac acaagaccag ctgggtgaaa accgccgccg tcggcaaccc gatgccgtct
     8581 gaacaggcca tcgcgaatgc gctgggtcaa atcaccaggg aactcgatgg ctgccgcgcc
     8641 ccggccgtcc ttttcctcgg gcaggctttt gtccagcctg aacggcaaac gatgattgaa
     8701 actttgtgcg gcagccgtcc cttcttcatc atcgaccatc ccgcccggct tttacgccaa
     8761 cccgaactta aagcccgaaa ctggcaggtg ttgaaacagt tgaaacgcgc cttacaggca
     8821 ggcggcagtt gaagcaggcc gcacggggcg gtagaatcac aactgcatcg caatacctga
     8881 cagaaagcaa aaaatgaccg atttccgcca agatttcctt aaattctccc tcgtccaaaa
     8941 cgtcttaaaa ttcggcgaat ttaccaccaa ggcaggacgg cagtcgcctt acttcttcaa
     9001 tgccggcctc tttaacgacg gcttgtccac gctgcaactg gcaaaattct atgcacaatc
     9061 catcattgaa agcggcatcc aattcgatat gctgttcggc cccgcctaca aaggcattat
     9121 tttggcggcg gcaaccgcga tgatgctggc ggaaaaaggc gtaaacgtcc cgtttgccta
     9181 caaccgcaaa gaagccaaag accacggcga aggcggcgtg ttggtcggtg cgccgcttaa
     9241 agggcgcgtg ctgattatcg acgacgtgat ttccgccggc acatccgtac gcgaatcaat
     9301 caaactgatt gaagcggagg gtgcaacccc cgccggcgtc gccatcgcgc tcgaccgtat
     9361 ggaaaagggc accggcgaat tgagcgcggt tcaggaagtg gaaaaacaat acggtctgcc
     9421 cgtcgccccc atcgccagcc tgaacgattt gtttatcctg ttgcaaaaca accccgaatt
     9481 cggacagttc ctcgaacccg tccgagccta ccgtcggcag tacggcgtag aataaaaaca
     9541 aagcatatgc cgtctgaacc accttacgcc tcagacggca tcaaacctga cacacacgag
     9601 gaaataccat gcccgcctgt ttctgccccc actgcaaaac ccgtctctgg gtcaaagaaa
     9661 cccagctcaa tgttgcccaa ggcttcgtcg tctgccaaaa atgcgaaggg atgtttaaag
     9721 ccaaagacca tctggcaagc acgaaagagc ctatattcaa cgatttgccc gaggctgttt
     9781 cggatgtcaa actcgttcac cgtatcggca cgcgcgccat cggcaagaaa cagatttccc
     9841 gtgacgaaat cgccggcatc ctcaacggcg gcacaaccca gcccgatatt ccgcccgcaa
     9901 ccgccgccac ccctgctgcc gcaccgcagg ttaccgtacc gcccgccgcg cccgcccgtc
     9961 aggatgggtt caactggacg attgcaaccc tgtttgccct tatcgtcctc attatgcagc
    10021 tttcctacct cgtcatccta tgatcgtgcc cgacctcttt gtcgcccact tccgcgaagc
    10081 cgccccctac atccgccaaa tgcgcggcaa aacgctggtc gccggcatag acgaccgcct
    10141 gctcgaaggc gataccttaa acaagctcgc cgccgacatc gggctgttgt cgcaactggg
    10201 catcaggctc gtcctcatcc acggcgcgcg ccacttcctc gaccgccacg ccgccgcgca
    10261 aggccgcacg ccgcattatt gccggggctt gcgcgttacc gacgaaacct cgctcgaaca
    10321 ggcgcagcag tttgccggca ccgtccgcag ccgttttgaa gccgcattgt gcggcagcgt
    10381 ttccgggttc gcgcgcgcgc cttccgtccc gctcgtatcg ggcaacttcc tgaccgcccg
    10441 tccgataggt gtgattgacg gaaccgatat ggaatacgcg ggcgttatcc gcaaaaccga
    10501 caccgccgcc ctccgtttcc aactcgacgc gggcaatatc gtctggctgc cgccgctcgg
    10561 acattcctac ggcggcaaga ccttacatct cgatatgctt caaaccgccg cctccgccgc
    10621 cgtctcgctt caggccgaaa aactcgttta cctgaccctt tcagacggca ttgcccgccc
    10681 cgacggcacg ctcgccgaaa ccctctcggc gcaggaagcg caatcgctgg cggaacacgc
    10741 cggcggcgaa acgcgacggc tgatttcgtc cgccgttgcc gcgctcgaag gcggcgtgca
    10801 tcgcgtccaa atcctcaacg gagccgccga cggcagcctg ctgcaagaac tcttcacccg
    10861 caacggcatc ggcacgtcca ttgccaaaga agccttcgtc tccatccggc aggcgcacag
    10921 cggcgacatc ccgcacattg ccgccctcat ccgcccgctg gaagaacagg gcatcctgct
    10981 gcaccgcagc cgcgaatacc tcgaaaacca catttccgaa ttttccatcc tcgaacacga
    11041 cggcaacctg tacggttgcg ccgccctgaa aacctttgcc gaagccgatt gcggcgaaat
    11101 cgcctgcctt gccgtctcgc cgcaggcaca ggacggcggc tacggcgaac gcctgcttgc
    11161 ccacattatc gataaggcgc gcggcatagg cataagcagg ctgttcgccc tctccacaaa
    11221 taccggcgaa tggtttgccg aacgcggctt tcagacggca tcggaagccg agttgcccga
    11281 agcgcggcgc aaagcctacc gcaacaacgg acggaactcg catattctgg tgcgtcgcct
    11341 gcaccgctga ccgcaacgga aagccgccgc agaaatgccg tctgaacccc gtttcagacg
    11401 gcatttcccc gattataaag acctgcccaa ccctcaagga ccccgatgaa atcctacccc
    11461 gacccctacc gccattttga aaacctcgat tccgccgaaa cgcaaaactt cgctgctgaa
    11521 gcgaatgccg aaacgcgcgc gcgtttttta aacaacgaca aggcgcgcgc gctttcagac
    11581 ggcattttga atcaaatgca ggacacgcgg cagattccgt tttgtcagga acaccgcgcg
    11641 cggatgtacc atttccatca ggacgcggaa tatccgaagg gcgtgtaccg cgtgtgtacg
    11701 gcggcgacgt accgttccgg ctatcccgag tggaaaatcc tgttttcagt ggcggatttc
    11761 gacgagctgc tcggcgacga tgtatatcta ggcggcgtgt cgcacttggt ggaaaagccc
    11821 aaccgcgcac tgttaacact aagcaaatca ggcggcgata cggcgtacac gctggaagtg
    11881 gatttggaag caggggagtt ggtcgaaggc ggttttcact ttccggcagg caaaaaccat
    11941 gtgtcgtggc gcgatgaaaa cagcgtgtgg gtgtgtccgg cttgggacga acgccagttg
    12001 accgaatcgg gctatccgcg cgaggtgtgg ctggtggaac gcggcaagag tttcgaggaa
    12061 agcctgcctg tgtatcaaat cgataaaggt gcaatgatgg tgaacgcgtg gcgttatctc
    12121 gatccgcaag gttcgccgat tgatttgatt gaagcgtcgg acggttttta caccaaaacc
    12181 tatttgcagg tgtcgtcgga aggcgaggcg aaaccgttaa acctgcccaa cgattgcgac
    12241 gtggccggct atctagccgg acatcttttg ctgacattgc gtaaagactg gcaccgcgcg
    12301 aaccaaagct atccgagcgg cgcgctggta gcggtgaaac tgaatcgcgg cgaaatcggg
    12361 gcggcgcagc ttttgtttgc gcccgatgaa acgcaggcat tggaaagcgt ggaaacgacc
    12421 aagcgttttg tggtggcgag cctgctggag aacgtacaag gccgtctgaa agcgtggcgt
    12481 tttgccgacg gcaaatggca ggaagtcgaa ctgccgcgcc tgccttcggg cgcgttggaa
    12541 atgaccgacc agccttgggg cggcgacgtg gtttaccttg ccgccggcga tttcaccaca
    12601 ccgctgacgc tgtttgcgct ggatttgaac gtgatggaac tgaccgtcat gcgccgccag
    12661 ccgcagcagt tcgattcaga cggcatcgaa gtgcggcagt tttggacgac ttctgccgac
    12721 ggcgagcgca ttccttattt ccacgtcggc aaaaacgccg cgcccgatac gccgactttg
    12781 gtctatgcct acggcggttt cggtattccc gaattgccgc attatctggg cagcatcggc
    12841 aaatactggc tggaagaggg caacgccttc gtgttggcga acatacgcgg cggcggcgag
    12901 ttcggcccgc gctggcatca ggcggcgcag ggcatcagca aacacaaaag cgtcgacgat
    12961 ttgcttgccg ttgtgcgcga tttgtccgaa cgcggcatga gtttgcccga acacatcggt
    13021 ttgcagggcg gcagcaacgg cggcctgatt accgccgccg ccttcgtgcg cgaaccgcaa
    13081 agcatcggcg cgctggtgtg cgaagtaccg ctgaccgaca tgatccgcta tccgctgctc
    13141 tccgccggcg caagctggac ggacgaatac ggcaatccgc agaaatacga agtctgcaaa
    13201 cgccagttgg gcgaattgtc gccgtatcac aatctttcag acggcatcga ttatccgccc
    13261 gcactcatca ccaccagcct cagcgacgac cgcgtccatc ccgcccacgc gctcaagttt
    13321 tacgccaaac tgcgcgaaac ctcgccgcaa tcttggctct actcgcctga cggcggcggc
    13381 cataccggca acggcacgca acgcgaatcc gccgacgaac tcgcctgcgt gttgctgttt
    13441 ttgaaagagt ttttgggcta agggcgggga gcggcactgc cgccgcgaat gaaaaaaggt
    13501 cgtctgaaac tgctttttca gacgaccttt tttaatggtt gtttcaaatc aaaatatcta
    13561 tgccgccggc cccatcagca cttcttcaca tccgaaggca aaaatccgta atgccgtctg
    13621 aacgcttcgt tgaaccgtcc cgcgtggcgg tagccgcaaa agtgcatggc ggcttggacg
    13681 gtgctgccgg attcgatgag ggcgagcgcg tgttccagcc gcaggcggcg caggcatccg
    13741 gcgacggttt cgccggtttg cgctttgaaa tagcgtttca ggtagcattc gttcagtccg
    13801 acgcggcggg cgatttcggc gatggtcagc ggacgggcga attcgtgttg caggatgtcg
    13861 gcggcttcgt ctatgcgccg acggcggtaa ccgttgtcgt ggcggcggaa ggtgaagcgc
    13921 aataatcggg cggagagttc cagcgcggcg gcttcgtcgg caagcaggcc gaagccgtcc
    13981 gattcgaacg ggcgttgcag cagtgggcag gccgccgccg tcagtgccgc tgcgttttgc
    14041 gccagccgtt gcagggcgaa tcggcctatc gtttgcggcg aaaacaggcg ttcgtccagc
    14101 aagccttcgt cgtgccagcg gcgcagtttt tccagcgaaa aatccaaatg cagcgcgcac
    14161 atgccgctgt tgtcgggcag cagggtttcg gatacgtccg ccaactcgcc gcgtaccagc
    14221 cagatttcgc cggcagatgg gcggtattcc ctgccgccca tttgtaaccg gttctgcccc
    14281 gacaccatga cgaacaaggc gcagttgtgg ctgaaattgt ggatttcggt gggaaacgcg
    14341 cccgttccgc cgccgcgcat ccgcgacaag gtgatgcccg agtcgaagcg gttgatgcac
    14401 atttccagat gcaaaccggg ctgttttgcc tgcgcaatga gcgcgctgtc ggaacagccg
    14461 tccaacgccc agccggattt atcggagcgg acataggttt ggtactggcg gtagatggcg
    14521 gcggtgttca tgattggata ggaacgagtt gtctaacaaa tgaattaaat aggaattatt
    14581 accaataatc aagcgcaggg attggttgaa acggaaaagg tcgtctgaaa gggtgtttca
    14641 gacgaccttt tccgtatcgg gaatttgttt tgccgtatcg ggaattttgc gttttgcggc
    14701 gtggtttctg caggttgttt gcttaataat aaacattctt attcgtatgc aaaggaaccg
    14761 cacaccgtga aaccgcgttt ttattgggca gcctgcgccg tcctgctgac cgcctgttcg
    14821 cccgaacctg ccgccgaaaa aactgtatcc gccgcatccg catctgccgc cacgctgacc
    14881 gtgccgaccg cgcggggcga tgccgttgtg ccgaagaatc ccgaacgcgt cgccgtgtac
    14941 gactgggcgg cgttggatac gctgaccgag ctgggcgtga atgtgggcgc aaccaccgcg
    15001 ccggtgcgcg tggattattt gcagcctgca tttgacaagg cggcaacggt ggggacgctg
    15061 ttcgagcccg attacgaagc cctgcaccgc tacaatcctc agcttgtcat taccggcggg
    15121 ccgggcgcgg aagcatatga acagttggcg aaaaacgcga ccaccataga tctgacggtg
    15181 gacaacggca atatccgcac cagcggcgaa aagcagatgg agaccttggc gcggattttc
    15241 ggcaaggaag cgcgcgcggc ggaattgaag gcgcagattg acgcgctgtt cgcccaaacg
    15301 cgcgaagccg ccaaaggcaa aggacgcggg ctggtgctgt cggttacggg caacaaggtg
    15361 tccgccttcg gcacgcagtc gcggttggca agttggatac acggcgacat cggcctaccg
    15421 cctgtagacg aatctttacg caacgagggg cacgggcagc ctgtttcctt cgaatacatc
    15481 aaagagaaaa accccgattg gattttcatc atcgaccgta ccgccgccat cgggcaggaa
    15541 gggccggcgg ctgtcgaagt attggataac gcgctggtac gcggcacgaa cgcttggaag
    15601 cgcaagcaaa tcatcgtcat gcctgccgcg aactacattg tcgcgggcgg cgcgcggcag
    15661 ttgattcagg cggcggagca gttgaaggcg gcgtttgaaa aggcagaacc cgttgcggcg
    15721 gggaaagagt agggagtcgt ctgaaaacgg agcttccgaa ggaagcgggg ggtttctgcg
    15781 aagctaaagt gcggtttcaa cgaattgaaa agcagcctgt atgttgaaaa taccgctcaa
    15841 gcaaacctac ggtttgccgc cctctcccta gccctctccc acagggagag gggattgggg
    15901 tgcaggctgt ctttaaggtt caggcaaatt tttaacttcg ttgaagctcc gttttcagac
    15961 gaccttttgg ag
//
LOCUS       DAXHSJ010000032        15898 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000032 DAXHSJ010000000
VERSION     DAXHSJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15898
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.032"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            38..1111
                     /locus_tag="ACFM8U_000740"
     CDS             38..1111
                     /locus_tag="ACFM8U_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC6334015.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKEQICTKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(1168..2694)
                     /gene="ilvA"
                     /locus_tag="ACFM8U_000741"
     CDS             complement(1168..2694)
                     /gene="ilvA"
                     /locus_tag="ACFM8U_000741"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC6334016.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            2842..4011
                     /locus_tag="ACFM8U_000742"
     CDS             2842..4011
                     /locus_tag="ACFM8U_000742"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC6334017.1"
                     /translation="MTAHKILPVLLSIILGISHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKTTEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWACLTGQ"
     gene            complement(4134..4706)
                     /locus_tag="ACFM8U_000743"
     CDS             complement(4134..4706)
                     /locus_tag="ACFM8U_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC6334018.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPVNIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(4773..5756)
                     /locus_tag="ACFM8U_000744"
     CDS             complement(4773..5756)
                     /locus_tag="ACFM8U_000744"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC6334019.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(5923..5998)
                     /locus_tag="ACFM8U_000745"
     tRNA            complement(5923..5998)
                     /locus_tag="ACFM8U_000745"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5963..5965),aa:Gln,seq:ttg)
     gene            complement(6007..6082)
                     /locus_tag="ACFM8U_000746"
     tRNA            complement(6007..6082)
                     /locus_tag="ACFM8U_000746"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6047..6049),aa:Gln,seq:ttg)
     gene            complement(6095..6170)
                     /locus_tag="ACFM8U_000747"
     tRNA            complement(6095..6170)
                     /locus_tag="ACFM8U_000747"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6135..6137),aa:Gln,seq:ttg)
     gene            complement(6178..7023)
                     /gene="ispE"
                     /locus_tag="ACFM8U_000748"
     CDS             complement(6178..7023)
                     /gene="ispE"
                     /locus_tag="ACFM8U_000748"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC6334020.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PWLSV"
     gene            complement(7033..7614)
                     /locus_tag="ACFM8U_000749"
     CDS             complement(7033..7614)
                     /locus_tag="ACFM8U_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC6334021.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            complement(7754..9592)
                     /locus_tag="ACFM8U_000750"
     CDS             complement(7754..9592)
                     /locus_tag="ACFM8U_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334022.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALRYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            complement(9757..10593)
                     /gene="panC"
                     /locus_tag="ACFM8U_000751"
     CDS             complement(9757..10593)
                     /gene="panC"
                     /locus_tag="ACFM8U_000751"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC6334023.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLSRELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            complement(10550..10726)
                     /locus_tag="ACFM8U_000752"
     CDS             complement(10550..10726)
                     /locus_tag="ACFM8U_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334024.1"
                     /translation="MQTEQGLLLMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARLA"
     gene            complement(10716..11507)
                     /gene="panB"
                     /locus_tag="ACFM8U_000753"
     CDS             complement(10716..11507)
                     /gene="panB"
                     /locus_tag="ACFM8U_000753"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC6334025.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDEAGAAVVLMECVPAQLAKKVTETVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMHGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            complement(11649..11724)
                     /locus_tag="ACFM8U_000754"
     tRNA            complement(11649..11724)
                     /locus_tag="ACFM8U_000754"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11689..11691),aa:Thr,seq:tgt)
     gene            complement(11759..11834)
                     /locus_tag="ACFM8U_000755"
     tRNA            complement(11759..11834)
                     /locus_tag="ACFM8U_000755"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11799..11801),aa:Thr,seq:tgt)
     gene            12000..12791
                     /locus_tag="ACFM8U_000756"
     CDS             12000..12791
                     /locus_tag="ACFM8U_000756"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC6334026.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            12846..13487
                     /locus_tag="ACFM8U_000757"
     CDS             12846..13487
                     /locus_tag="ACFM8U_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6334027.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(13565..14488)
                     /locus_tag="ACFM8U_000758"
     CDS             complement(13565..14488)
                     /locus_tag="ACFM8U_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6334028.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGATLDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            14652..15521
                     /locus_tag="ACFM8U_000759"
     CDS             14652..15521
                     /locus_tag="ACFM8U_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC6334029.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKILSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGRQPDRTDGKRESSRNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            15564..>15898
                     /locus_tag="ACFM8U_000760"
     CDS             15564..>15898
                     /locus_tag="ACFM8U_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334030.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGEWIPNAVGGIMGGVGGHFSYMASAIASGSYNREAHWTAIGGGALIG
                     AMNPINGGRA"
BASE COUNT         3997 a         4595 c         3695 g         3611 t
ORIGIN      
        1 aagacaaaaa actcgccgcc gccgaaagga acgcaatatg agtatcacca tccaaaactt
       61 aaacaaacac ttcggcaatt ttcacgcgct gaaaaacatc aacctcaacg tccccaccgg
      121 caaactcgtt tccctactcg gcccgtccgg ctgcggcaaa accacgcttt tacgcattat
      181 cgccggactg gaaaacgccg acggcggcaa gattctgttt gatgggcaag acgtaaccgc
      241 caagcacgtg cgcgagcgca aagtcggctt cgtgttccaa cactacgccc ttttccgcca
      301 tatgaacgtg tttgacaacg tcgctttcgg tttgaccgta ttgcccaagt ccgaacgccc
      361 gtccaaagaa caaatctgca ctaaagtcga agaattgctg aaactcgtac aactctcgca
      421 tttggcaaaa tcctatccgc accaactctc cggcgggcaa cgccagcgca tcgccctcgc
      481 ccgtgcgctt gcggtcgaac cgaaactcct gcttttggac gaacccttcg gcgcgttgga
      541 cgccaaagta cgcaaagaat tacgcacctg gctgcgcgac atccatcaca acctgggtgt
      601 aaccagcatt ctggttacgc acgaccaaga agaagccctc gaagtttccg acgaaatcgt
      661 cgtgatgaac cacggcaaaa tcgaacaaac cggcagcgcc gaagctattt accgcaaacc
      721 cgaaaatgcc ttcgttaccg agttcctcgg cgaaaccgac gcatttgaag gacgcatcga
      781 aaaaggcttc tggcattata acggcttcgc gtggaaattg gacgcacaat acaaatggca
      841 ggaacaaacc gccaccggct atatccgccc gcacgaatgg cagatcgccg ccgaacacga
      901 aacaccgatg atttgtgccg aaatcgaaaa agtccacgcc gtcggggcat tgacgcatat
      961 tttggtgaaa cacggcaaac aagacgtgca catcacgctg gctggcagcg atgccgcgcg
     1021 ttacccaatc gccgaaggca aagaattgaa gctgattccg aaacaggttt atgtcttctc
     1081 tcaaaacgaa ctgattgaat attcgattta accataaaag cgcaatgccg tctgaaaggc
     1141 tttcagacgg cattgcgctt tcaagcgtca ggcaagaaac agcttgtacg cggcattttg
     1201 cgtttcctcg tgatagctgt atcccagact ttccaagaaa ccgtcaaatg cggcggcatc
     1261 gtgcggcggc acatcgatac cgaccagaat ccgcccgtaa tccgcaccgt ggttgcggta
     1321 atggaagagc gtaatattcc accctccctg catatggttc aaaaagcgcg ccaacgcccc
     1381 cggacgctcc ggaaactcaa aactgaccaa acgctcgttt tctactttgt ccgtccgccc
     1441 tccaaccata taacggatat ggattttggc aatctcatca tcagtcaggt cgacattggg
     1501 caatcccgcc tcatccaacc ggctaccgat aaccgccaaa tcctgcgggc ctgccgcttg
     1561 aagtccgaca aagatatgcg ctttttcatc gtctccgtag cggtagttga actcggtaat
     1621 attcctattt cccaatatat tgacaaactt aaggaagctg ccgcgttctt cggggatggt
     1681 aacggcaaaa ataccttcgt tgccctcgcc caattcacta cgctccgaca catggcgcaa
     1741 acggtgaaaa ttcatattcg caccgctggt aacggcaatc agggtttggt tttccgcgcc
     1801 ttctcgggcg atataggctt tcagacccgc caacgccaac gcgcccgccg gctcggtaat
     1861 gctgcgcgtg tcatcgaaaa tatccttgac cgcgccgcaa accgcatcgg tatcgactgt
     1921 aatgatttca tccaaaagtt ctttgcagag acggaaggtt tcgtttccga cgactttgac
     1981 cgccgtgccg tctgaaaaca gcccgacatc tttcaaatgg acgatttcgc ccgcttcaac
     2041 cgattgcttc atacagcagg aatcgttggt ctgaacgccg ataactttga tttcggggcg
     2101 gacctgcttg ataaatgccg ccacgcccgc cgccaaaccg ccgccgccta tcggtacgaa
     2161 tacggcgcgg attggatcgg gatgctggct gacaatttcc atccccaccg tcccctgtcc
     2221 cgcaatcaca tcaggatcat caaacggcgc gatataggtt aacccttctt tttccgccaa
     2281 ctccatcgca taatcgtagg catcgttgta tgaaacgccc cgcaaaacca cctcgccgcc
     2341 acggcttttg accgcatcca ctttgatttt cggcgtagtc tccggcatga cgataacggc
     2401 acggcaaccc aaacgctgtg cggacaatgc cacgccttgt gcgtgattgc ccgcgcttgc
     2461 cgcaatcacg ccgcaagcga gcgcatcttt cggcaacttg gacattttgt tgtacgcgcc
     2521 gcgtattttg aacgaaaaaa ccggctgcaa atcttcgcgt ttcaaaagga tgttgttttt
     2581 caaacgtaca gaaaggctgc gtgccggttc caaaggcgtt tcgaccgcga catcatagac
     2641 agatgctgtc aggatgcgga tgaggtaatt ggaataagga aggggcgtgt tcataattca
     2701 atatgggata atcggtttat taaaatcgca aaacccaaaa ccatacgccc aagacggcgc
     2761 gaaagcaaga aaaatccgcc cgatcagaca ccctaagcgt ataatcggca ggctgaaaca
     2821 ctcacacaat tagaatattt catgacagca cataaaatcc tgcccgtcct gctttccatc
     2881 atcttaggca tttctcacgc aacggccgca tcgcccgcgc ccaacaaacc ggcggtacac
     2941 gccgccccca cgttccaaac acccgaaacc ctcacagcgg cacacatcgt tatcgacctt
     3001 caaagcaaac agattttatc cgccaaaaac atcaataccc ctgtcgaacc ggcggcacta
     3061 acccaactga tgaccgcata tctggttttc aaaaacatga aatcgggaaa tatccgatct
     3121 gaagaaaact taaaaatacc cgaatccgca tgggcttcag aaggaagcag aatgtttgta
     3181 cgtcccggcg atacggtcag caccgacaaa ctcttaaaag gcatgattgc actatccgca
     3241 aacgatgccg ccctaaccct tgccggccga ctgggcaacg gctcgattga aaattttgtg
     3301 caacaaatga acaaagaagc ccgacgcttg ggcatgaaga acactgtatt caaaaacccg
     3361 acaggcttga gtagagaagg acaggtttcc accgccaaag accttgccct gctgtctgaa
     3421 gcattgatgc gcgactttcc ggaatattac ccgctgtttt ccatcaaatc tttcaaattc
     3481 aaaaatatag aacaaaacaa ccgcaatatc cttttatata gggacaacaa tgtaaacggt
     3541 ctgaaagccg gacacacaga aagcggcggc tacaaccttg ccgtgtcata ctccggcaac
     3601 ggcaggcaca tccttgtcat cacattgggt tcggaatcgg cggaaacacg cgcatcagac
     3661 aacagcaagc tgctgaactg ggcattgcaa gccttcgata cgcccaaaat atatccgaaa
     3721 ggcaaaaccg ttgcccaaat ccaaatttcc ggaggcagca aaaaaaccgt ccgcgcaggc
     3781 ttcctcaaag aagcctacat cactctgcca cataaggaag cgaaaacaac cgaacaaatt
     3841 ttggaaacca tacagccgat tcccgcccca gtaaaaaaag ggcaaatttt aggaaaaatc
     3901 aaaatcagac aaaacggata caccattgcc gaaaaagaaa tcgtcgcact ggaaaatgta
     3961 aaaaaaagaa gccggtggca aaggctttgg gcgtgtctga cagggcagta atctgccgtt
     4021 tcaaatatcc cgtttttcca acaaataaag aaatgccgtc tgaaacacgg ttcagacggc
     4081 ataaaacaac agggcggtac gcatcatata cgtaccgcct gcttttcaat caattagcgt
     4141 tttttaccgg taacagtggc aacagccaga ttttcgttac gtttcaggga aacgctttct
     4201 acaccttcag gcagtttgat gtctgacaag tgcagaatgt cgccggcaac cacttcggca
     4261 caatccaaat ccaagaaagc agggatgttg acaggcaaag caactacttc aacagaagtg
     4321 tttaacagag atacgcggcc gccttgcagt ttgaccgctt gggaattttc agcgttaacg
     4381 atgtgcaggg gaacacggat acgtacaagt tgatcggctt tcacagcttg gaagtcgatg
     4441 tgttggactt cgcggcggaa cgggtgcatt tggaaatcgc ggacgataac gtctttggtt
     4501 tcgccgttca gagacaattt aatcaacgca gtatggaaag attctttttc caatgcgtag
     4561 aatacagttt tgtgatccac agcgattgca acgggctctt ggccttcacc gtacaaaatg
     4621 ccggggattt ggccttcgcg acgcaggcgg cggctcgcac cagtgccttg tgcttcacga
     4681 acagaggctt gaatttcata agtcatgtta aatactccaa tttaaataaa actaccgtca
     4741 tcggccgcga ccagcttaag acggcttcgg gcttatggca gcaacatact gcctgtcatc
     4801 acttcttcat taaagagata tgaaacggat tcttcattgc tgatgcggcg gacggtttcg
     4861 gccaacagac cggcaatcgt tacctgacgg atacggtcgc agtttttagc cgcttcagac
     4921 aaaggaatgg tatcggttac gaccacctgg tcgatttcgg atgaggcgat acggctgacc
     4981 gcctctccgg agaatacggc gtggctggca tatgccaaga cacgttccgc cccccgctct
     5041 ttcagagcga cggcggcttt gcacagcgta tttgcagtgt caatcatatc gtccacaatc
     5101 agacaggttc tgccttgaat atcgccgatg atgttcatga cttccgccac attggctttc
     5161 gggcggcgtt tgtcgatgat tgccaagtcg gcattcaggg attttgccac ggcgcgggcg
     5221 cggacgacac cgccgatgtc cgggctgacg acggtcagat tttcaatccg ctgttgtttg
     5281 atgtcgttca acagaatcgg ggtggcataa atattgtcca ctggaatatc gaagaaacct
     5341 tgaatctggt cggcatgcaa atcgacagtc aaaacacggt cgatccctgc cgaatacagc
     5401 atatttgcca ccagtttggc agaaatcgga acgcggacgg aacgcggacg gcggtcttgg
     5461 cgcgcatagc cgaaatacgg aatggctgtg gtaatacgac ctgccgaagc acgcttcagt
     5521 gcatccgcca tcgtcaggat ttccatcagg ttgtcattgg tcggcgcaca ggtcggctga
     5581 aggatgaaaa catcgcgccc gcgtacgttt tccaacagtt cgacggcaac ttcgccgtct
     5641 gaaaacttgg atacggaagc attgcccaaa gaaatgtcca aatgcctgac aacacgttgt
     5701 gccaattcgg gattggcatt gcctgtaaat accatcaaac tgtcgtacgc agccatattc
     5761 tcacctgatt ttatgtttaa cttccgctca gaaaacacaa tgcttccctc tgcttgtacc
     5821 aagcccgaca ttatacctgc aacatccgac acaaacaaag gcatttcaat atttttattt
     5881 ctatgaaata aaagcgtgta agcaggctta cacgctttta tctggctggg gaggaaggat
     5941 tcgaaccttc gcatgctgga atcaaaatcc agtgtcttaa ccgcttgacg actccccaaa
     6001 aagggctggc tggggaggaa ggattcgaac cttcgcatgc tggaatcaaa atccagtgtc
     6061 ttaaccgctt gacgactccc caactcgttt gacttggctg gggaggaagg attcgaacct
     6121 tcgcatgctg gaatcaaaat ccagtgtctt aaccgcttga cgactcccca acaatgttta
     6181 tacggacaac caaggatgtt ttgaaagacc ctctgccaaa tatgcctcgt acaaatctga
     6241 aacttgtcgg tatatattgt atgcgctatt cctatcttga cacgccgtga atacacacgc
     6301 accagaacct gtcattaagg caaatccata tcgggacaat tcggaatagg ctttccaaac
     6361 ttcagggtat tctttaaata caactgcctg catgtcattt ctaaacggtt gcaggttttg
     6421 gaaagtcggc attatgcttg aggcggaatt tcgtgtcaag ccttcgtgtg tgaaaatttt
     6481 tgcagtggaa acgtggacgg gcggtttgac gatgacatac cactgtttcg gaatatccat
     6541 ttcgtccagc ctgtcgccta taccccgcgc aaacgcattt ttgccgaaaa taaaaaacgg
     6601 tacgtccgcc cccagagccg cgcccgaatc aatgagctgc cgctgcgtca gaccgcactg
     6661 ccaccaacgg ttcaacacca gcaaaacggt tgccgcatcc gagcttccgc cgcccaaacc
     6721 cgcccctgtc gggatttttt tgtccagcca tatttccacg ccggcggggt tgcgcgcata
     6781 tttttgcagc aacgatgcgg cacggtagct caaatcggct tcctgcggta ttccgccgac
     6841 aggattgtgc aggatgattt tgccgtcgtc cctcggtttc aaatatacgg tatcctgcaa
     6901 atctatcagg cagaatatgc tttcgatatt gtgataaccg tcttcccgct ttccagtaat
     6961 cctcaaatcg agattcagtt ttgcaggtgc gggaaacgcc tgccgtccgt ccgcaatatt
     7021 catctgtccg ccttatctcg tgcgcgccgc acagcgttcc ggggtttcgg tttcagacgg
     7081 cataccgatt tcggtgaaaa ccagcctgat gttcaaattt ccgttattca gttgcaacgt
     7141 tcggacttgc cccccactgt cggcggttct gccgacagtc caaccgtatt gttccaatat
     7201 gccgtccggc aggatgcggt aaggcgcgcc cgccacacgc ctgccatctg cccagatatg
     7261 cagatattgg attggcagtt tgaaaccgac cagctgcctg ctcaattctt ccgcactttc
     7321 cgcctgatag acatttcctt tgccgtccac tgccaatgcg ccgtccctgt cttgacacaa
     7381 ctgcccgagc gtactgccca aaggggtatt gatattgatg gtttccacgg gcggttggta
     7441 tgtccaatcg aaatttgcat acgaaccttt cccttccgct ttcactgcca accgcccttc
     7501 cgccgtgaaa ctgcgggtgt gttcggacgg ctgccacagg ttttcgttat tttgaggtaa
     7561 ttgcgcgcaa gcggtcaaaa gcaggatgac cgatgcggat acggtgtgtt tcatcagaat
     7621 ttccttaacg gatgccatcc tgccggccga tgggttcggc gtgccgtgca aaacgcgtgc
     7681 gaactgcgtg ccgtttgccc gaatgccccg gcttcccggc aaaccttatg ccgtctgaaa
     7741 ggatggacct gcattatttc cggggtttgc gggaaggttg gggcaatgcg atgccgtgac
     7801 gtttgagcgt ttcccgccat attttcttgt ctcccgtaag gtgtgccgcc tgcgtccata
     7861 cgtcaaccgc ctgatcgcgt tcgcccaatg cccacaacac ttcgcccaaa tgggcggcaa
     7921 cttcgggctc ggggtcgttt tcaaacgaat accgcagata cggcagcgcg ctttccgcgt
     7981 cgcctttcag gtaatacgcc cagcctatgc tgtcgttgac agcggtatcg tccgggttga
     8041 tttggtatgc cgtctgaagc agggcgaagc cttcgtccaa acgtttggaa tcggaaagca
     8101 ggctgtagcc cagattattc ataatctgag cgttatcggg tgcaagcctg aacgcccttt
     8161 caagatctga aatcattttt ttccgcttgc caagccgatc gtaaacaact gaccgctgta
     8221 ccaatgcctc tgcctgtaac tctgtattac tgccggcagg cggtttttcg ataatcttgt
     8281 ccaaccccct caaagcctcc cgtttgtcgg gcagcttcga cagggcgaac atctgtattt
     8341 tggacaaatt gtctgccgta aaataccgcc cctgctgttc gggaagtttc cgcaccctgc
     8401 cgatctgccg caaagccgcc ctgccgccgt ccaactcgac agccgccgca gccgccagca
     8461 cacctttgtc gaacaggtat tccggagcgg acactttttt caaccactgc ctgactttgg
     8521 tgtaatccct tcggtcggca tatatcatcg ccgccgtcat tgccgccctg ccccgctgtt
     8581 cccccgtccc cctgccgtat gccttttcgg cgtagccgtc gataacggaa gcaccttctt
     8641 ttcggtttgc cgccaatatc gctgcctgaa tatacaggtc tgcattcgga ttgcgttcca
     8701 acagcacgtt caaacgcgca taggcatcat ccagcctgtg cagggaaacc agattcataa
     8761 tttccatttc ctgccagacg gccgaaaggt tttgggtgtc tgtctgctcg aaaaagccgt
     8821 cgagtatttc gggatatttg cgtgcagtca gacgcaacgt cattaaagtg gggggcaata
     8881 tttccgtatc gagcttcgcc aaacgctgca aagctccgat tgccttttcc ttttcgcgtc
     8941 cctgtacgct gaacaccaca tcggcaaccg ccgcttcggg cagatgttca tatctcaacg
     9001 ccgcgcggcg aaccgctttc gatgcttttt gcgccaaccc gtcctgttgc acggcggctt
     9061 gtgccaacaa taaaaacacc ctgcggttct gtccttcgtc cgcctgagcc agcacttctt
     9121 ccagtccgtc tagatgctga tttcctcttt ccctcagcac gttccgcagc caccccgccc
     9181 gtttttgcgc cttacccggt ataggctcaa tctgccgcca tttctgataa atcatttccg
     9241 cctgttcaaa cgcgttcagc gacacggcca tttccaaggc gcgttcggcg acttcggggg
     9301 attttgtgcg ttccaacatc agcatatagg ttgccagagc cgttcccgcc tgcccctttt
     9361 gcaaggcggt ttcccctccc agcaacgtaa atatctgatt aacccgctcg cccactgccg
     9421 caagccgtgc gcgttcgttt ttgatttctt cctcgctgta acgctgctgc tttctgaaaa
     9481 cctttccgac ttccttcggc ggcttcgcat ccgccgcgcc ggcggcatac gcctgcccgg
     9541 caagcagggc tgccgcgagc acagataaaa tggtgaaacg ggcgggtaac ataatcttcc
     9601 tttatccgac agccgtccga accttcagac ggcatataat caggcgtaac tttatcacaa
     9661 gcagaatcgt ttgtagattt gcgaaacccc gttcaaaaaa ataaatgccg tctgaaacgc
     9721 aaatccgctt cagacggcat tcccgacgct tgcggtttaa gggagtttta tttccaaatt
     9781 gtcaatcagg cgcgtcgtcc ccagacgggc ggcggccaag accaccagtt tcttatctcc
     9841 cgcccgcgcc acttcgagcg tatcggcgcg gcggatttcg acataatcga ccacccagcc
     9901 cgcagcggtc aaatggtcgg cagcctgccg ctccaaacct gcataatcca aactgccctg
     9961 cgccaaggat tcggcaacag cctttaattc gcgggacagg cgcggtgctt cgtcgcgttc
    10021 cgccgcactc aaatactggt tgcggctgga gagtgccaaa ccgtcttccg cgcgccccgt
    10081 atcgacaggc actatttcaa catcaaaatt caaatcctcg gtcaaacctt taatcacggc
    10141 aagctgctga tagtcttttt tgccgaaaca ggcaacatcc ggcgcgacaa tattgaacag
    10201 cttggacact accgttgcca ccccgcggaa atgtcccgga cgaaacttgc cgcacaactc
    10261 attttgcagg ttgggcggtt cgacgttgta acgctgttcc acgttcggat agagttcttt
    10321 ctcatcgggc gcgaaaacaa cggcaacacc ctcggcttcc agcttgtccg catcctgttg
    10381 caaagtacgc ggatatttgt cgaaatcctc gccctgaccg aattgcaggc ggttgacgaa
    10441 tatgctgaca acaacgttgt cggcgcgttt tttggcttcg cgaacgagag caagatgtcc
    10501 ttcatgcaga ttgcccatgg tcggcacaaa tgccaccttt cccgcatttt tacgccaggc
    10561 gcgcagttct cgaatggtat gtatgatttg cataacggta atccttatcc ttcgggcttc
    10621 ctgcccgaca aaacgcgccg attatacgcg ccgcgcacgg caaaacaaaa tgccgtctga
    10681 aacggctttc agacggcatc agcagcaaac cttgttcagt ctgcaaaaat atgttccgca
    10741 gcagggaagg ttttggcttt gacttcggca acatacgccc gaaccgccgc ctgaatgctg
    10801 ctttgcccgt gcataaagtt tttgacgaat ttcgccgtct tgcccggaaa aataccgagc
    10861 atatcgtgca tcaccaaaac ctgcccgtcg caatcgacac ccgcgccaat gccgatggtc
    10921 ggacaggaaa cagtttcagt tacctttttt gccagttgcg caggaacaca ctccatcagc
    10981 acgacggcag ctccagcttc atcgtgcgcc ttggcatcgt taagcaacgc ctgcgccttg
    11041 ccgccgcgcc cctgaacttt atatccgccg aaggcaaaca cggattgcgg cgtcagaccg
    11101 atgtgcgcgc aaaccggaat accgcgcatt tgcagaaatt cggtcgtttc cgccatccac
    11161 acgccgcctt cgagtttgac catatgcgcg ccggcagcca tcagttcggc ggcggcggca
    11221 aacgcctgct ccttactctg ctgatatgca ccaaacggca aatcgctgac aatcatcgca
    11281 ttttttgcac cgcgtgctac acattcggtg tgatagcaca tatcgcgcag gctgacgggc
    11341 agcgtcgatt tccgcccttg aaccgccata cccaaagagt cgccgaccag cagcatttcc
    11401 acgccggcat cgtccatcag tgcggcaaaa ctggattcgt aagcggtcag catagcgatt
    11461 ttttcgcccg ccgccttcat tttttgcagt gtgttcacag taatcatcag atattgttac
    11521 cccgcccttt tcagacggcc ttttcagttt gaaacgggct attatagtga aatgcccgca
    11581 gccctgccac cgaccgtgcc gcaaatgcaa aacagcccga ctgttttaca caatcggact
    11641 gtcaaatctg gtgccggcac caagagtcga actcgggacc ccctgattac aagtcaggtg
    11701 ctctaccaac tgagctatac cggcttactg aaatgttttg caacaccatc agcagaaatg
    11761 gtgccggcac caagagtcga actcgggacc ccctgattac aagtcaggtg ctctaccaac
    11821 tgagctatac cggcaaagaa agcgaattat gcaggcaatg ccgcaccttg gcaaggaatt
    11881 tatccgaaag ccccggcaaa tccgaataag taatttattt gaaaagaaat taatttgaag
    11941 caggacaagc atcggcattc tgataaaata tccgcctttc aaaccgaccg ttcacaccga
    12001 tggcaagaca tccctaccgc cgcctgcgtc ctgcaaaatc cggctttccc gaagtcggca
    12061 tttccgaaga aggcaatatc cgttcgcttc acttgggcag cgacaccgtt caaagctcaa
    12121 tgaacctcga ccacccgtcc gagctggtac tctcttacag ccgcgcgatg atgggctggc
    12181 tgctgtttac cgacgcactt ccgcagcata tcacccaaat cggcttgggc ggcggctcgt
    12241 ttgcacgctg gatagacacc tacctgcccg acacgcgcca aaccgccgtg gacatcaatc
    12301 ctcaggtcat cgccattgcc cgcaacctgt ttgagttgcc tttcgagggc gagaaatttg
    12361 aaattattga agcagacggt gcagaatata tcaaagtctt ccgccacaac accgatgtga
    12421 ttttggtgga cgggttcgac ggcgaacaaa tcatcgatgc gctggttgaa gaaccgtttt
    12481 tccgagactg ccgaaacgca ctctcttcag acggcatatt cgtaaccaac tggtggagcg
    12541 gcgacaaacg ctaccaacgc ttcatcgaac ggttgttgag cgtttttgaa gggcgcgtct
    12601 tggaacttcc tgccgaaagc cacggcaatg tcgcggtaat ggccttccaa agcagcccca
    12661 aagagcaaaa catagacaaa ctcaaaaaac gtgccgacaa actgagcaac gcatacggat
    12721 tggacttcca ccgtatgctt gccggcctga aagcgtccaa ccccaacaac ggcaagcatt
    12781 tccaccttta agcaactatc ttcacggctg cattggaaca tgcggaaaat caagaggaaa
    12841 tcattatgct gaaacccgaa accatacaca tatccggtcc tgccggcatt ttagaaacca
    12901 tccatatccc gtccgaacaa gtaccggcac gcggtgttgc cgtcatcaat catcccaacc
    12961 ccctccaggg cggaacaaac accaacaaag ttatccaaac tgccgccaaa gccttaagca
    13021 aactcggctt ccactgctac ctacccaacc ttcgcggcgt aggcggcagc gaaggcacac
    13081 acgattacgg acgcggcgaa acgcaagact gcctcgccgt catcgactat gcccgtgccc
    13141 aacatcccga agcccccgaa tttgccttat ccggcttctc cttcggcggt tatgtcgcca
    13201 catttgccgc acaagcgcgc atacccgatt tattgctgct catcggcgcg gcagtctgcc
    13261 actataccgg ccgccccgag ccgtccgccg ttcccaatgt tgccaaaacg ctgatgatac
    13321 acggcgcgga agacgaagtc gtcgaaatcg ggaaagcact gaaatgggcg gaaccgcaag
    13381 atttgcccgt cattaccatc gccggttcca cgcatttctt tcacggcaaa ctcatcgtcc
    13441 tgcgcgacac catcctccgc ttcgcgcccg tatgcctgaa cggataaaac ccctttcaga
    13501 cggcatcgat aatgtaaaac ccgtatcggc ggcatccgcc gatacgggtt tgcgttccct
    13561 ctcattatag gcaaaatccg ctgcaaaaca ccctttcccg acccgacaaa ggatctgcaa
    13621 acgcaatctt ttttgccaat agcttcaaag gtttccgata atcctcgctg cccgcttcag
    13681 acggcacggg atagagcgcg tcattcaaca gcggcatacc caaacccatc atatgcacgc
    13741 gcaactggtg tttcttgccc gtatgcggcg taaggcggta aaggctgaat tcccccctgt
    13801 tttcaatcag ttcgaccgtc gtatgtgcgt ttggttcgcc ttccgcctct tgcgtcgtaa
    13861 aaaatttctc acccctcacc aaacgcgaaa ccacatcgag cggatacggc aaatccgtcc
    13921 ttgtcggcgc aagcgcctca tacgttttcc ataccgtttt gttttgaaac atcgtctgat
    13981 aggctccgcg cgtggcagga ttatgcgaca gcagcatcac gcctgccgta tccttgtcca
    14041 agcggtgcag cggcgtaatg tcctcaacat tcaaatgctg caattcaggc cgcaaacgca
    14101 ggcgcgtgag cagggtttcc cgcaaaaacc tgccgctggg gatgacgggc agaaaatgcg
    14161 gcttgtccac cacaatcaaa tgctcatcaa tatgcaaaat cttttcttca aacggaatac
    14221 gcggctcatc ctcactgctg gtttcccgat aataaaacat caccttaccc ggctcgaaca
    14281 aagtatgttc gtccaacgtc gcaccatccg aaccgaccac aaaaccgctg ttcaaccgcc
    14341 tgcgccaatc gtccgcgccc acaaaaggaa agcggatgca cagaaaatgc agcagcggca
    14401 gcccgtaaaa ctgcttttca tgcggcagca ccaaataact gggtttgaca ccgttcaaca
    14461 gaggaagagg attattgcgt tttttcatgc ggcggattgt aaccgttttt cacaaacttt
    14521 tcagacggca tatcgaaaca taattgacaa aaacaaacat attgtttttt atgatttaaa
    14581 aatttattta gacaagatta tcggatattc attattatca gtaaataaga attaatatca
    14641 ataggagaaa tatgaagcgc atctttttgc ccgccttgcc cgccatcctg cctttatcca
    14701 cttatgccga cctgcccttg acgattgaag acataatgac cgacaaggga aaatggaaac
    14761 tggaaacttc ccttacctac ctgaacagcg aaaacaaccg cgccgaactt gccgcaccgg
    14821 tttacattca aaccggcgca acctcgttta tccccattcc gaccgaaatc caagaaaacg
    14881 gcagcaatac cgatatgctc gtcggcacgc tcggtttgcg ctacggactg accgggaata
    14941 ccgacattta cggcagcggc agctatctgt ggcacgaaga acgcaaactc gacggcaacg
    15001 gcaaaacccg caacaaacgg atgtccgacg tatccctcgg catcagccac actttcctta
    15061 aagacgacaa aaaccccgcc ctaatcagct ttcttgaaag cacggtttac gaaaaatcgc
    15121 gcaacaaagc ctcgtcggga aaatcctggc tcatcggcgc caccacctac aaagccatag
    15181 atccgattgt cctttccctc accgccgcct accgtatcaa cggcagcaaa atcctttcag
    15241 acggcatccg ctacaaatcg ggcaactacc tgctgctcaa ccccaacatc tcatttgccg
    15301 ccaacgacag aatcagcctg accggaggca tccaatggtt gggcaggcag cccgaccgga
    15361 cggacggcaa acgggaatcc tccagaaaca catccaccta tgcccatttc ggcgcaggtt
    15421 tcggtttcac caaaaccacg gctttaaacg catccgcacg tttcaacgtt tcagggcaaa
    15481 gcagttccga actgaaattt ggcgtacagc atacgtttta agcaggtttt gattttccaa
    15541 ccttgtaatc caaaggagtt attatgaaaa aacaaatcac tgaagccacg atgatgctgt
    15601 ctataatcgc ccccgcaatg gcaaacggct tggacaatca ggcatttgaa gaccaagtgt
    15661 tccacacgcg ggcagatgcg ccgatgcagc tggcggagct ttctcaaaag gagatgaagg
    15721 agactgaggg ggagtggatt cctaatgctg ttggaggcat tatgggagga gttggagggc
    15781 atttcagcta tatggctagt gctattgcta gtggctcgta taatcgagag gcacattgga
    15841 cagcaattgg aggtggtgcg ttaattggtg cgatgaaccc tattaacgga ggaagagc
//
LOCUS       DAXHSJ010000033        15402 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000033 DAXHSJ010000000
VERSION     DAXHSJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.033"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(105..1889)
                     /gene="lpdA"
                     /locus_tag="ACFM8U_000761"
     CDS             complement(105..1889)
                     /gene="lpdA"
                     /locus_tag="ACFM8U_000761"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6334031.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGYKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1907..2344)
                     /locus_tag="ACFM8U_000762"
     CDS             complement(1907..2344)
                     /locus_tag="ACFM8U_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334032.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2438..4045)
                     /gene="aceF"
                     /locus_tag="ACFM8U_000763"
     CDS             complement(2438..4045)
                     /gene="aceF"
                     /locus_tag="ACFM8U_000763"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC6334033.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAASAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSVIIEVETVGSAAATPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQ
                     GGAAKPAAAGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4195..6858)
                     /gene="aceE"
                     /locus_tag="ACFM8U_000764"
     CDS             complement(4195..6858)
                     /gene="aceE"
                     /locus_tag="ACFM8U_000764"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC6334034.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7552..9264
                     /locus_tag="ACFM8U_000765"
     CDS             7552..9264
                     /locus_tag="ACFM8U_000765"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC6334035.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEVNSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANVELAPTLPLKGERTAAQAELTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVESENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAIAIGEIVARVTA
                     SLNA"
     gene            9327..9983
                     /locus_tag="ACFM8U_000766"
     CDS             9327..9983
                     /locus_tag="ACFM8U_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC6334036.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGQDADGMGEGEDILGCVAGYGVGLDLTA
                     RDIQCHLKAQGLPWLKAKGFRHSACVSDFAAADRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9993..10448)
                     /gene="ruvX"
                     /locus_tag="ACFM8U_000767"
     CDS             complement(9993..10448)
                     /gene="ruvX"
                     /locus_tag="ACFM8U_000767"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC6334037.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTEHEMTHLSRKFGRRLHGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGRKG"
     gene            complement(10441..10989)
                     /locus_tag="ACFM8U_000768"
     CDS             complement(10441..10989)
                     /locus_tag="ACFM8U_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC6334038.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPVGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10991..11551)
                     /locus_tag="ACFM8U_000769"
     CDS             complement(10991..11551)
                     /locus_tag="ACFM8U_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC6334039.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11879..13684
                     /locus_tag="ACFM8U_000770"
     CDS             11879..13684
                     /locus_tag="ACFM8U_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6334040.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13811..15295
                     /locus_tag="ACFM8U_000771"
     CDS             13811..15295
                     /locus_tag="ACFM8U_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC6334041.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3686 a         3790 c         4266 g         3660 t
ORIGIN      
        1 gccggaggaa atattttaat ccgctgtaaa atagaaaatg ccgtctgaac atttcagacg
       61 gcatttgctc aaataaacct tatcggctgt ttattcagtc ggatttattt tttcttttgc
      121 ggaggcaggt cggtacaagt acccaatgcc acttccgcag ccataccgat ggattcgccc
      181 aaggtcgggt gcgggtggat ggttttgccg atgtctgccg cgtcgcagcc catttcgatg
      241 gcaaggcaga cttcgccgat catgtcgcca ccgttcggac cgacaatgcc gccgccgatg
      301 atgcggccgg tttcagcatc aaaaatcagc ttggtgaaac cattgtcgca accgttggca
      361 atcgcacggc cggaagctgc ccacgggaag ttggctttgg tgattttgcg gccggatgct
      421 ttggcggaca actcggtttc gcccacccac gccacttcgg gggaagtgta ggcaacgcca
      481 ggaatcacgc gcgcgtcgaa gtaggctttg tagccggcgc agttttcagc ggcaacgtgg
      541 ccttcgtgaa cggctttgtg cgccaacatc ggttgaccga cgatgtcgcc gatggcgtag
      601 atgtgcggca cgttggtacg catttgtttg tccacttcga tgaagccgcg atcggttacg
      661 gcaacgcctg ctttttccgc gctgatgagt ttgccgttgg gcgcgcggcc ggcggcaacc
      721 aatacggcat cgtagcgttg cggctctttc ggtgcatttg caccttcaaa ggtaacgtaa
      781 acgccgtctt ctttcggctc gactgcaacg gttttggtgt tgaccataat gttgtcaaaa
      841 cggtattcgt tttgtttttg ccatactttc accaagtcgc ggtctgcgcc ttgcatcagg
      901 ccgtccatca tttcaaccac atccaaacgc gaacccagcg tgctgtaaac cgtacccatc
      961 tcgaggccga taatgccgcc gccgataatc agcagtttgc ccggtacttc tttcagagcc
     1021 aatgcgccgc tggaatcgat gatgcgcgga tcttcaggaa tgaaaggcag tttggttacg
     1081 cggctgcctg ctgcaatgat acagtttttg aaggcaacga tttttttctc gccggtagtg
     1141 gctgcctgtt cgtacgcgtc gccggcagtc agcgacactt ccaagtggtg cggatctaag
     1201 aattgcccgt cgccttggat aacgtccact ttacggcttt tcgccatacc tgccaaaccg
     1261 cccgtgaggc gggaaactac gccgtctttg taggcgcgaa gcatatcgat gtcgagttcc
     1321 ggctcggggt atttgatacc gttggcagcc aagtggcgca cttcgtcgat aacggcggca
     1381 ttgtgcaaca gggctttgga agggatacag ccgacgttca ggcaaacgcc gcccaaagtt
     1441 ttgtaacgct cgacgatggc gactttcaag ccttcatcgg cagcggcaaa tgcagcggag
     1501 taaccgccag ggccgccgcc caatacgacc acgtcgtact cggcatcggc agaaccgccg
     1561 aattgcgcgg cttgcggagc aggagcggca gctttagggg cttcttgcgc cggggcggca
     1621 gccgcttcgg ctttaggggc ggcagccgtg ccttcagctt caacgacaac aatcaaaccg
     1681 ccttcggaga ttttgtcgcc gactttgact ttcacttctt tgaccacgcc cgcaacttcg
     1741 gcaggcacgt ccatcgtcgc tttgtcggtt tccaaggtaa tcagggtatc gtccacagca
     1801 atagtgtcgc ccacgttcac ttcaactgcg ataatatcta cattttcgtg tccgccaatg
     1861 tcgggcactt tcaattcaac taaagccatt atattgacct ttcaacttag ttaatttcta
     1921 tcaactcttt agcaataatc acttcttcag gaatacagcc acaatcttca gctctgattg
     1981 ttacttcctt ctcatttggg ttttctggat gttcaacctc atattcaaaa atagaatatt
     2041 gaccaaacaa atccctattt aaaacatata tatagccatt ttcgatgccg gaacttgttg
     2101 caaatttctt ggcaatttcc ttgtctgttg tcgtagatat ataacatccg tcatatagac
     2161 ctgtttcaat ttgatgggcg taaactgcat tcttcacact tggaccatgt gtagctttac
     2221 catcatattt aaacttacca tcataacgaa ttgcaacttc agctttatta cctttaggtt
     2281 ttaactgtcc attattttgc tcatcttgtt ggcaactaat gcctctatat aagaaatttc
     2341 ccataagtta ttaatttaaa ggttgaattt aacaccatta ctcatcaatc tcaagcaatc
     2401 caaacattat cagattgctt gagagggatg ttttatttta taaggtaatg cggcggaagt
     2461 ctttcaatag cttcgccaag aatacggtga agcgcatacc ggccgcaccg tcgatgacgc
     2521 ggtggtcgaa ggacaggctc aacgggcaca tcaggcgcgg ggcaaactct ttgccgttcc
     2581 aaaccggttt gatttgggat ttgcacacgc ccaagatggc gacttcggga gcgttcacaa
     2641 ttggcgtgaa gcctgtgccg ccgatgccgc ctaagctgga aatggtaaag cacgcgcctt
     2701 gcatttcttg cggtttgagc ttgccttcac gggctttttt ggacaattcg gtcaattctt
     2761 ggctgatttg tttcaaacct ttttgatcca cgtctttgat gacgggaaca accaagccgt
     2821 tcggcgtatc ggctgcgaaa ccgatgttga agtagttttt cagcaccagg ttgtcgccgt
     2881 ccagagaagc gttgaattcg gggaaggctt tcagcgcgga aacggaggct ttgatgatga
     2941 acgccagagg agacagtttc acgccttcgc gttcccattc tttgttcagt tgtttgcgga
     3001 attcttccag ctcggtcata tctgcttctt cgtgtacggt aacgtgggga atcaccaccc
     3061 agttgcgaga caggttttga ccggagattt tcttaatgcg ggacaattct ttaacttcga
     3121 cattgccgaa tttggagaag tccactttag gccacggcag caagtccaga ccgctgccca
     3181 aagatgcgcc ggctgcggca ggttttgccg cgccaccctg cattacggat ttcacaaagg
     3241 ctttgatgtc gtcgcccacg atgcggcctt tcaagccggt acctttgact tggcccaaat
     3301 ccacgcccaa ttcgcgcgcc agtttgcgtg cggaagggcc ggcgtgtgct ttggcgaaag
     3361 cggcctcgtc gattttggca gcggcaggtg cagaaggtgc aggcgcggcg gcgggtgcgg
     3421 cggcagcagt cggaggcgga gcggcagccg gtgcggcagc ttgagcggct tgagcagggg
     3481 ttgctgcggc agagccgacg gtttctactt cgataatgac agagccttcg gatactttgt
     3541 cgccgacttt taagaatacg gctttaacga caccggcagc ggtacaaggt acgtccattg
     3601 tcgctttatc ggtttccaaa gtaatcagcg tgtcgtcttc ggcaacggtg tcgcccactt
     3661 tgatttcaac ggcgatgaca tccacatcgg tatggccgcc gatatcggga acggctactt
     3721 gaacggttgc accgcctgcg gcaacgggtg cggcagcagg tgcaggttgt gcttcggcag
     3781 cagccgaggc ggcttcggca gcggcggcac cggtttcaac ggtcaaaatt acgccgcctt
     3841 cggagatttt gtcgccgact ttgactttta cttctttcac gacaccggca gcatcggcag
     3901 gcacatccat cgtggctttg tcggtttcca gtgtaatcag ggtgtcgtca acggcgatgg
     3961 tgtcgcccgc tttaacttct acggcgatga tgtcgacgtt ttcgtgaccg ccgatatcgg
     4021 ggactttgat ttctacgata ctcatttgag ttcctttaat cttgttcagt tggatgccgt
     4081 ctgaaagctg cttcaggcgg catcggtacg gtttgtctgc tcaaaccatt cgggcattcg
     4141 gttttcagac ggcctgatgt cggaatgggg caaacagacc gtctgaaaca tcaatcagcg
     4201 tttccagcta ggagctgaat cggctttgat gccgtatttc tcaatggctt gttgaacggt
     4261 ttctttgctg actttgcctt gttccgccaa tgcgctcaat gctgccacgg caacgttgta
     4321 gcgatccact tcaaagaagc ggcgcaggtt ggcgcggctg tcggagcggc cgaagccgtc
     4381 agtacccaag acgtggtagt cgttcgggat gtaggcgcgg atacggtcag catagctgcg
     4441 gatatagtcg gtagcggcaa taaccggacc gtcatgacct tgcagttggg aagtaacgaa
     4501 aggtactttt tcggcttcca gcggatgcag gcggttgaag cgttctacct cgacagcgtc
     4561 gcggtgcagc aggttgaagg atgggcaaga ccaaatgtct gcttccacgc cgaagtcggc
     4621 tttcagcagc tcggcaccgg caatcacttc ttgcaaaatc gtaccggagc ccatcaattg
     4681 aactttcttg tcgcctttgc cgccggcttt cagcaggtac atacctttca agatgtcttg
     4741 ttccgcacct tcgggcatat ccggatgggt gtagttctcg ttcatcaggg tgatgtagta
     4801 gaacacgtct tcattattgg catacatacg gcgcagaccg tcttgtacga tgacggcgac
     4861 ttcgtattgg aaagtcgggt cataagatac gcagttcgga atcaggtcgg cctggatgtg
     4921 gctgtggccg tcttcgtgtt gcaggccttc gccgttcagc gtcgtacggc cggcagtacc
     4981 gcccagcagg aagccgcgcg cgtgcatatc gcccgccgcc caagccaagt cgccgatacg
     5041 ttggaaaccg aacatagaat agtaaatgta gaacggaatc atcgcgaagt tgctgttggc
     5101 gtagctggtt gcagccgcaa tccagtcggc catcgcgccc ggttcgttaa taccttcttg
     5161 caagatttga ccgtcaacgg attctttata gaacatcagt tggtctttgt cttgaggggt
     5221 atattgctga cctttcggat tccaaatacc gtattggcgg aacatacctt ccatgccgaa
     5281 agtacggctt tcgtcgggaa cgataggtac gacgcgtttg ccgatttttt tgtctttcag
     5341 caaagtggac aggatgcgga cgaatgccat cgtggtcgag aactcgcgtt caccgctgga
     5401 tttgagttgt gcgtcgaatg ctgacagctc gggcacttcc aatacttcct gcgtcggttt
     5461 gcgttgcggc aggtagccgc ccaaagcatc gcggcgtgcg tgcaggtatt tgtattcttc
     5521 cgtatcgggg gcaaaagtca ggtaaggcag atcgccgctt tcgatttgtt cgtcggtaac
     5581 cggaatgtca aagcggtcgc ggaattgttt cagggacgct ttgtccattt ttttggcttg
     5641 gtgggcaacg ttctgacctt cgccggatgc gcccataccg taacctttaa tggttttcgc
     5701 caagatgacg gtaggtttgc cgtcggcatg gttcgctgcg cggtcgtagg cgttgtacac
     5761 tttttgcggg tcgtggccgc cgcggttcaa tgcccagagt tgctcatcgg tcatatcggc
     5821 aaccaatgct ttcagttcgg gcgtattgaa gaagtgttcg cgcacatacg cgccgtcttt
     5881 ggatttgtaa gtttggtagt cgccgtccaa acattcttcc atacgttggc gcaggatacc
     5941 gtctttgtct ttcgccaaga ggcggtccca gcggcggccc caaatgactt tgacgacatt
     6001 ccagccggcg ccggcaaagt tgccttccaa ttcttggatg attttgccgt tgccgcgtac
     6061 cggaccgtcc aagcgttgca gattgcagtt gatgacgaaa atcaggttgt ccaagccttc
     6121 gcgtgcagcc agtgcgattg caccttgaga ttcgggttcg tccatttcgc cgtcgccgca
     6181 gaaacaccat actttacggc ctttggtttt tgccaaacca cgcgattcca agtatttcag
     6241 gaaacgcgcc tgataaatcg ccatgatggg ccccaagccc atggatacgg tcgggaactg
     6301 ccaaaagtcg ggcaagaggt gggggtgcgg ataggaaggc agaccgtgtc cgtccacttc
     6361 ttggcggaag ttgttcagct ggtcttcggt cagacggccc tcgacgaatg cgcgtgcata
     6421 gatgcccggg gcgacgtgac cttggaagaa gaccaaatcg ccttcttcgc cttcgccttt
     6481 ggctttccaa aagtggttga aaccgacttc gtacatggtg gcggcagatt ggaaagatgc
     6541 gatgtgccca cccagttcca aatctttctt gccggcgcgc aatacgatgg cggcggcgtt
     6601 ccagcgcacg aatgcgcgga tgcggtgttc gatgttttgg tcgcccggaa tgcctttttc
     6661 gttttcaacc gaaacggtat tcaaatacgg ggtggtcgtg ccgtgtggca tacgtacgcc
     6721 cttgtcgcgg cagtatttga ccaggttttc caagaggtat tgtgcgcgtt cgccgccttc
     6781 atattcgagg acggagctta acgcgtccag ccactcttgg gtttcgatag ggtcaacatc
     6841 gtgtaattgg gtggacatag tatctatcct ttatgttgag tgtttaatcg gcagggaggg
     6901 tgtagccaac ccctttattt tcgtttgtga aaaataataa gcaaaaacga aaatttctaa
     6961 atcaaaccga aaaaaataat ttaagtttaa tgttttcaaa aaattataaa agttttggaa
     7021 acaagggaaa acttgaaaaa aatgttcgat ctgctccaca cgaaatccta tcagcaatcg
     7081 ggtctgctgg caaataacca tgcctgtaaa agcaggtttt atactgaagt tggcgtttgg
     7141 taaggtcaag tctttttgat ttcgaaatat tctgtttgat tttggcgata ttttttcgga
     7201 attgtggtat atcgaatcat tatgtctctg tcggcaattt tcctattgta gaaaatctat
     7261 acacaagctt tgtggataac cgtgttgata agccacggct tgtgttaaaa aagtcttacg
     7321 caacaaatgt acgcttattt tgattaaaaa ataggcacat ttaaatctat aaaaatcatg
     7381 tgcttatgtt caatatgatg tttctggtca tgtttttgcc gtctgttatg ggatttgtcc
     7441 cgcgcttggg gcagggggta aattttggag gttggtgcaa aacctgcttt tttcgcgtta
     7501 caatctgcac ccgatttact tttgaatttg cctgtttgga aaaatatttt tatgaaagcc
     7561 agccaattct ttatctctac tttaaaagaa gcccctgccg aagccgcgct tgccagccac
     7621 aagctgatga tacgcgccgg tctgattaaa gccaacgcgt ccggccttta tacttggatg
     7681 ccgatggggc tgcgcgtgtt acgcaaagtc gaaaacgtcg tgcgcgagga aatggcgcgc
     7741 gcgggcagcg tggagctgct gatgccggtg gtgcagcctg ccgagctgtg gcaggaatcc
     7801 ggccgctggg aattttacgg taaagaactg ctgcgcctga aagaccgcca cgaccgcgat
     7861 ttctgcatgg gtccgacttg tgaagaagtg attgcggaca tcgtgcgtaa agaagtcaac
     7921 agctacaaac aattgccgaa aaacttttac cacatccaaa ccaaattccg cgacgaagtg
     7981 cgcccgcgct tcggtgtgat gcgtgcgcgc gagtttgtca tgaaagatgc ttattccttc
     8041 catgccgact acgcctcgct tcagacgacc tatgatgcca tgtatgacgc ttactgccgt
     8101 atcttcaccc gtctgggctt ggcattccgc cccgttgccg cagataccgg cagcatcggc
     8161 ggtaccggct ctcacgagtt tcaagtgttg gcggaaagcg gcgaagatgt gattgcatac
     8221 agcgacactt ccgattacgc cgccaatgtc gagctggcac cgaccttgcc gcttaaaggc
     8281 gaacgcaccg ctgctcaggc cgagttgacc aaagtacata caccaaatgt caaaaccatt
     8341 gagtctttgg ttgaattcct gaatattccg gttgaacaaa ccctcaaatc catcgtggtc
     8401 gaaagcgaaa acgaaggcga aatcgtctta ttactgttgc gcggcgacca tgagttcaac
     8461 gacatcaaag ccgaaaaact cgcgggcgtg aaatcgccat tgacgatggc aagtcctgcc
     8521 gcgattgtcg aacagttcgg cgcaaacggc ggctcgctcg gccctgtcgg cttcacaggc
     8581 aaagtctatg ccgatttcgc taccgaaaaa ggcgcggact gggttatcgg cgcaaacgaa
     8641 gacgactacc actataccgg gttcaatttc ggccgagacg ctgccgagcc tgagtttgtc
     8701 gatttgcgca acgtcgtcga aggcgacgaa agccccgacg ggcaaggccg tctgaaactg
     8761 gcgcgcggca ttgaagtcgg acacgtcttc cagttgcgtg acaaatatac taaagcgatg
     8821 aatgtcagct tcttggacaa caacggcaaa tcgcaaatta tggaaatggg ctgctacggc
     8881 atcggcatca cccgcgtcgt tgccgccgcc atcgagcaga ataacgacga aaaaggcatc
     8941 atctggacca aagcaatggc gccgtttgaa gtcgttatcg tgccgatgaa ctacaaaaaa
     9001 tcagacaccg tacgcgaagc cgccgacaaa atctatgccg aattgctggc ggcaggcgcg
     9061 gatgtgctgc tggatgaccg cgacgaacgc gcaggcgtat tgctgaacga ttccgagctt
     9121 ctcggtattc cgcaccgcat cgtcatcggc gaccgcgcct tgaaagaagg caatgtcgaa
     9181 tacgccgaac gccgcgacaa cgaagcgcag gcaattgcaa tcggagaaat tgttgcacgt
     9241 gtaacagctt cattaaatgc gtaagcttgg tcgaatgccg tctgaaaata atttcagacg
     9301 gcatttttac ttagggggaa agtgaagtgg cttcagtatt tttagaagga gaggcagtcg
     9361 aggtcggcaa tattttctgt atcggcagga actatgccgc acatatcgaa gagttaaaaa
     9421 acgaaatacc ttccgaacct gtggtgttta tgaagccgtc aggcagcatt ttgaatagcg
     9481 gcggtacaat cttgctgccc gagtttagta gggatgtgca gtttgagtgc gaactcgtgt
     9541 tgctggtcgg tcaggatgcg gacggcatgg gcgagggtga agatattttg ggatgcgtgg
     9601 cggggtacgg cgtgggtttg gatttgaccg cgcgggacat ccaatgccat ctgaaggcgc
     9661 aggggctgcc ctggctgaag gcaaaagggt tcaggcattc cgcgtgcgtg tcggattttg
     9721 cggcggcaga cagaatcggc aatcccgaga aggtgttgtt ttcattaaaa caaaacggcg
     9781 tattgaaaca gcgcggggat accggtttga tgatttaccc gattcgggaa attttacaca
     9841 aactggcggc ggattacggt ttggggaaag gggatttggt gtttaccgga acgccctccg
     9901 gcgtgggcgc aatcggtgcg ggggacaatt tggcattgga gctggacggc ttggtgcgcg
     9961 cctctttcac ggtagggtgt taaccgcgcc gcttaaccct tacgcccgtt gaaacattcc
    10021 gccggaccgc cctcgaaaaa accgtgcaag atggcttgcg ccgccacttg gtcgagcacc
    10081 gatttgcgtt ttttgccgaa gacctgtgct tccgaaagca ggctttcggc atagacggat
    10141 gacagccgtt cgtccaccca atagacgggg agattgaacc tgccgtgcag cctgcgcccg
    10201 aacttgcggg acagatgcgt catttcgtgt tccgtgccgt cggtatgcac gggcagtccg
    10261 acgacaaaat aacgcggctg ccattcttga accagcttgg cgattgccgc gaacttttca
    10321 tcgttgctgc cgccggtaac ggtgctcaaa ggatgggata gccctaattc cgcgtctcct
    10381 tgtgccacgc cgatacgcgc ttcgccgaag tcgaatgcca gtgccgttcc ttttggaatt
    10441 ttatgcatgg ccggctcccg aaaacagggc gagcgggtcg atgccgagtt tggcaaaggc
    10501 ggcggcgtaa cggtgttcgt aggggatgtc gaacaagata tgttcgtcgg cgggaacagt
    10561 cagccacgca ttgtcggcaa gttcgcgttc gagctggcct tttccccagc ttgaatagcc
    10621 tatgctgatc aaggctttgt caaccgcacc ttcgcgtgaa atgttttcaa gcacatcgcg
    10681 ggaagaggtc agcgcgatgc cgtctgaaac gccgatgctg ctttgccagt tgccgaccgg
    10741 cgtgtggacg acgtaaccgc gctcgacctg caccggcccg cccatcatca cgctgtcgtg
    10801 ctgcatccgc atggggatgt ttttgccggt ggcggaaaaa atcatgtcca tcgtaatcgg
    10861 agagggtttg ttgatggcga tgccgagtgc gccgtcttca tcgtgtttgc agatatagac
    10921 gaccgattgt gaaaaaaacg cgtcttccat atcgggcatg gcgacgagaa agtggttgga
    10981 gaggttcata tcagcgtttg tcgggatttt ctatcgggtt ggaaatcatg caggcgccgg
    11041 caagcaggtt gccggaagta tcggcttgca ccccatcggt ttgcggtatg ccgccgccga
    11101 aacaggaaac cgcgcttaag gaatttttcg gcgagccttg gatgatttta tcgctgtaca
    11161 ccaaataggc gaaggttttg cgtttggggt cgtaataacg gacaatctgc cggctcttga
    11221 acgcgaagct cgcaccgtgt ttgaaaactt ctttcggttt gcgcacggcg gtttcgtcaa
    11281 aagaaatcga agatgccgtc tgaacgcacg aaaccgatgc gtcggacgcg tcctcttcca
    11341 aattgaccat ttccttcaag ccgccttttt ttgcatacga aatataacag gcaacccctt
    11401 gaacgtcggg atcgtcgaat ccttccactt cgatacggtc gtttttgccc agcatgttga
    11461 aaacggtact tgcccgtccg attttatcgg tttcgccgct gccgcaggca gtcagcagga
    11521 cggcggcaga cagcagtagc agtctgttca tcaaaatttc cttatcggtt gaatccccgt
    11581 tttccgtgaa ggcggcaggg cttgctttgt ttggaggggc gcacgttccc aaaatcgggc
    11641 aacatacggt atgcggctta atgtgctacc ctgtgtgtcg gaacacaata tccgttgcgg
    11701 cattgaaaat atgacgcaca atataaggct gtttgagccg attgcaagta tccgcgcaaa
    11761 gaaggcgcaa gattgggcaa acggcatttt cagacggcat aactgacagt ataatccaaa
    11821 cgtccggctg ctttcgtgca gccttcagta ttatccaact ttttccgaag gtcgaaccat
    11881 gcgctacaaa ccccttctgc ttgccctgat gctcgttttt tccacgcccg ccgttgccgc
    11941 ccacgacgcg gcacacaacc gttccgccga agtgaaaaaa cagacgaaga acaaaaaaga
    12001 acagcccgaa gcggcggaag gcaaaaaaga aaaaggcaaa aatggcgcag tgaaagataa
    12061 aaaaacaggc ggcaaagagg cggcaaaaga gggcaaagag tccaaaaaaa ccgccaaaaa
    12121 ccgcaaagaa gcagagaagg aggcgacatc caggcagtct gcgcgcaaag gacgcgaagg
    12181 ggataagaaa tcgaaggcgg aacacaaaaa ggcacatggc aagcccgtgt ccggatccaa
    12241 agaaaaaaac gcaaaaacac agcctgaaaa caaacaaggc aaaaaagagg caaaaggaca
    12301 gggcaatccg cgtaagggcg gcaaggcgga aaaagacact gtttctgcaa ataaaaaagt
    12361 ccgttccgac aagaacggca aagcagtgaa acaggacaaa aaatacaggg aagagaaaaa
    12421 tgccaaaacc gattccgacg aattgaaagc cgccgttgcc gctgccacca atgatgtcga
    12481 aaacaaaaaa gccctgctca aacaaagcga aggaatgctg cttcatgtca gcaattccct
    12541 caaacagctt caggaagagc gtatccgcca agaacgtatc cgccaagagc gtatccgtca
    12601 ggcgcgcggc aaccttgctt ccgtcaaccg caaacagcgc gaggcttggg acaagttcca
    12661 aaaactcaat accgagctga accgtttgaa aacggaagtc gccgctacga aagcgcagat
    12721 ttcccgtttc gtatcgggga actataaaaa cagccagccg aatgcggttg ccctgttcct
    12781 gaaaaacgcc gaaccgggtc agaaaaaccg ctttttgcgt tatacgcgtt atgtaaacgc
    12841 ctccaatcgg gaagttgtca aggatttgga aaaacagcag aaggctttgg cggtacaaga
    12901 gcagaaaatc aacaatgagc ttgcccgttt gaagaaaatt caggcaaacg tgcaatctct
    12961 gctgaaaaaa cagggtgtaa ccgatgcggc ggaacagacg gaaagccgca gacagaatgc
    13021 caaaatcgcc aaagatgccc gaaaactgct ggaacagaaa gggaacgagc agcagctgaa
    13081 caagctcttg agcaatttgg agaagaaaaa ggccgaacac cgcattcagg atgcggaagc
    13141 aaaaagaaaa ttggctgaag ccagactggc ggcagccgaa aaagccagaa aagaagcggc
    13201 gcagcagaag gctgaagcac gacgtgcgga aatgtccaac ctgaccgccg aagacaggaa
    13261 catccaagcg ccttcggtta tgggtatcgg cagtgccgac ggtttcagcc gcatgcaagg
    13321 acgtttgaaa aaaccggttg acggtgtgcc gaccggactt ttcgggcaga accggagcgg
    13381 cggcgatgtt tggaaaggcg tgttctattc cactgcaccg gcaacggttg aaagcattgc
    13441 gccgggaacg gtaagctatg cggacgagtt ggacggctac ggcaaagtgg tcgtggtcga
    13501 tcacggcgag aactacatca gcatctatgc cggtttgagc gaaatttccg tcggcaaggg
    13561 ttatatggtc gcggcaggaa gcaaaatcgg ctcgagcggg tcgctgccgg acggggaaga
    13621 ggggctttac ctgcaaatac gttatcaagg tcaggtattg aacccttcga gctggatacg
    13681 ttgatcggat tgccgaaacg gtaggcttta tgcaaatcgt gccgatatgg tgcgctgttc
    13741 cgtgaagttt gccgtttgcc gtacctgtat tttccggttt ttggaaatca agagagaaag
    13801 ttaaagaaga atgtcgaaac ctgtttttaa gaaaatcgca ctttatactt tgggtgcaat
    13861 cagcggcgtg gccgtcagtc tggcggtgca gggttttgcc gccgagaagg acaggcggga
    13921 taacgaagtc ctgccggtgc aatccatccg cacaatggcg gaggtttacg gtcaaatcaa
    13981 ggcaaactac tatcaggaca aacccgatgc cgatttgttt gaaggtgcga tgaagggtat
    14041 ggtggccggt ttggatccgc attccgaata tatggataaa aaaggttatg ccgagataaa
    14101 ggagtccacc agcggcgaat ttggcggctt ggggatggaa atcgggcagg aagacggatt
    14161 tgtcaaagtg gtttcgccga ttgaggacac gcctgcggaa cgggcggggg tgaaaagcgg
    14221 cgatttcatt gtgaaaatcg ataatgtttc gacacgcggc atgacggtca gcgaagcggt
    14281 gaagaaaatg cggggcaagc cgggtacgaa gattactttg acgctgtcgc gcaaaaatgc
    14341 cgacaagccg atagtcgtca acctgacccg tgccattatt aaagtgaaaa gcgtccgcca
    14401 tcacctgatc gaacccgatt acggctatat ccgcgtgtcg cagttccaag agcggacggt
    14461 cgaaagcgtc aataccgccg caaaagagct ggtaaaggaa aataaaggaa aaccgctcaa
    14521 ggggctggtg ttggatttgc gcgacgaccc cggcgggctt ttgactggcg cggtcggcgt
    14581 gtcggcggca tttctgccgt ctgaagcagt cgtcgtcagc accaagggac gcgacggcaa
    14641 agaccgcatg gtactgaaag ccattcctga agattatgtg tacgggatgg gcggcgattc
    14701 gttggcgggc attcctgccg agttgaaaac cataccgatg acggtattgg tcaattccgg
    14761 ttcggcttcc gcgtcggaga ttgtcgcagg tgcattgcag gatcataaac gcgcggtcat
    14821 cgtcggtacg cagagcttcg gcaaaggttc ggttcagact ttgattcctt tgtccaacgg
    14881 cagcgcggtc aagctgacaa cggcactgta ttatacgccg aacgaccgtt ctattcaggc
    14941 gcaggggatt gttcccgatg tcgaagtaaa agataaggaa cgcatttttg aaagccgcga
    15001 ggcggatttg gtcggacaca tcggcaatcc tttgggcggc gaggatgtga acagtgaaac
    15061 ccttgccgtg ccgcttgaaa aagatgcgga taagcccgct gtaaaagaaa aaggtaaaaa
    15121 gaaaaaggac gaggatttgt cttcaaggcg gattcccaac cctgccaaag acgaccagtt
    15181 gcggaaagct ttggatttgg tcaagtcgcc cgagcagtgg cagaagtctt tggggctggc
    15241 ggcgaaaaag ccggtttcaa ataaagataa gaaagataaa aaagataaga agtaggtttt
    15301 tacgatgccg tctgaaagga tttcagacgg catattatgt ttgaaaggac aggttatgag
    15361 cgatttttta ccggaatttg aaccgtgcgg catttatagt gg
//
LOCUS       DAXHSJ010000034        15286 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000034 DAXHSJ010000000
VERSION     DAXHSJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.034"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(84..3161)
                     /locus_tag="ACFM8U_000772"
     CDS             complement(84..3161)
                     /locus_tag="ACFM8U_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFC6334042.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALSGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGITVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILSCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEHAAGLSDEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDECMSAPFALDFI
                     CQGSWGDLGVDADKSEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRHRVRPNT
                     GATHGVYAGSCQEEPDGDLSSPLVRGHKEPDWQAYDEKGNRTVYAEECRNAKKIKTEP
                     KLDAEGKQVYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGSPVFTGFSGTVPVWK
                     TVKVADDHVPALYNYAKYLNTNKTHSLTASTRFNVTGRLHLLGGLHYTRYETSQTKDM
                     PVRYGQPASDFQTASSIKADQDHYTAKMQGHKLMPYAGITYDLTPQQSIYGSYTKIFK
                     QQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVDFGYVPR
                     AGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVSYNKSRYKNAAEVNAERLAKN
                     TGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNIRQGGYGLIDG
                     FVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            3435..4214
                     /locus_tag="ACFM8U_000773"
     CDS             3435..4214
                     /locus_tag="ACFM8U_000773"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6334043.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            4227..4367
                     /locus_tag="ACFM8U_000774"
     CDS             4227..4367
                     /locus_tag="ACFM8U_000774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334044.1"
                     /translation="MSDAFGWTVKPTRHENRVGKNAVWKNSDGIFGHITCKRQHIPTQ
                     TD"
     gene            complement(4425..5810)
                     /locus_tag="ACFM8U_000775"
     CDS             complement(4425..5810)
                     /locus_tag="ACFM8U_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFC6334045.1"
                     /translation="MIVASRWPLLEKAFLRFGMPVAVVDLESTGGNLYEDRVTEVALV
                     KFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELFSVLKGCVLV
                     AHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSIIERLGIVVED
                     RHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLREQLYGLPDG
                     MGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFPALGSLHAYK
                     IKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVTDNPPTGLFA
                     NKKAARRALSLWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACGRSDEQVLAF
                     AHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGFWYFDKDLLDAFKAKF
                     KTDRKNIKEIG"
     gene            5928..6004
                     /locus_tag="ACFM8U_000776"
     tRNA            5928..6004
                     /locus_tag="ACFM8U_000776"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5962..5964,aa:Arg,seq:acg)
     gene            6022..6096
                     /locus_tag="ACFM8U_000777"
     tRNA            6022..6096
                     /locus_tag="ACFM8U_000777"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6055..6057,aa:Glu,seq:ttc)
     gene            6655..7830
                     /locus_tag="ACFM8U_000778"
     CDS             6655..7830
                     /locus_tag="ACFM8U_000778"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC6334046.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(8041..8589)
                     /locus_tag="ACFM8U_000779"
     CDS             complement(8041..8589)
                     /locus_tag="ACFM8U_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC6334047.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDVAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(8592..10088)
                     /gene="ccoG"
                     /locus_tag="ACFM8U_000780"
     CDS             complement(8592..10088)
                     /gene="ccoG"
                     /locus_tag="ACFM8U_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC6334048.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLAVVIAAFLVGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            10618..12597
                     /gene="tkt"
                     /locus_tag="ACFM8U_000781"
     CDS             10618..12597
                     /gene="tkt"
                     /locus_tag="ACFM8U_000781"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC6334049.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFILSNGHASMLLYSLLHLTGYNLGIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEKGAKLEAEWDKLFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            12742..14130
                     /gene="fumC"
                     /locus_tag="ACFM8U_000782"
     CDS             12742..14130
                     /gene="fumC"
                     /locus_tag="ACFM8U_000782"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC6334050.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(14195..15118)
                     /locus_tag="ACFM8U_000783"
     CDS             complement(14195..15118)
                     /locus_tag="ACFM8U_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6334051.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
BASE COUNT         3844 a         4375 c         3535 g         3532 t
ORIGIN      
        1 cgccccgata gacctgcctc cataaaaatg ccgtctgaaa cataccctgt ttcagacggc
       61 atccgcaaaa ctacggtttt caattaaaac tgccaatcca gtttcatgct gacagtgcgc
      121 ggctctccgt agaagttgtt tgcgccgcgc gtacggttgt agttgttctc aaaataagtg
      181 cgtccgttta agttcgtacc gatgaggctc aatttggcgt gtttgcccaa ttcgtaacgg
      241 acgaaaccgt ctatcagccc gtagccgccc tgacggatgt tgtacaggct gcttgtgccg
      301 ctttgtgcgg acacgccgcc gccgacggtc agccccgtat tcggtatatg gaagctcgtt
      361 ccgaaacgga atatgtgcac gggtgtgaaa ttgctgaagt tgtacgggtc tgcgcctgtg
      421 tttttggcga ggcgttcggc gttgacttcg gcggcgtttt tgtagcggct cttgttgtag
      481 gagactttcc aatcttcgtt caactcaccc gacaactcga attccgcacc cctgctgacc
      541 actttgccta tcggtttggc aacggtttgg aacgacccct gcttgccgcc tgctctggga
      601 acatagccaa aatcgacgac cgtgcggttt ttctgttcga ggtaaaacaa tgcgaacgaa
      661 gcattcagcc gtccttgcaa gaacgcgcct ttccagccta cctcatagtt tgtgccgacc
      721 aaaggcggta aaacggtttt ggcactgaca tcgacattat cctgctgttt gaagattttg
      781 gtataacttc cgtaaatact ctgttgcggt gtcaaatcat aggtaatgcc tgcatagggc
      841 atcaatttat gaccttgcat cttggccgta taatggtcct gatccgcctt aatgctcgat
      901 gccgtctgaa aatcgcttgc cggctgccca tagcggacag gcatatcttt ggtttgcgag
      961 gtttcatagc gcgtgtagtg cagcccgccc aaaaggtgca gtcggccggt tacgttgaaa
     1021 cgcgtgctgg cagtcagcga atgggttttg ttggtgttga ggtatttggc gtagttatac
     1081 agcgcaggaa catggtcatc ggccacttta acagttttcc aaaccggcac cgtaccggaa
     1141 aaaccggtaa agacaggcga gccgtcggga ttggtctcct gaatcttgtt gcctttttcg
     1201 tccagctcat atacatcgac atataccggt gtccggctgc cgctgtattc gtcatagtaa
     1261 tacacctgct tgccttcggc atcgagcttg ggctcggttt ttattttctt ggcattcctg
     1321 cattcttcgg cataaacggt acggttgcct ttttcatcgt acgcctgcca atcgggttct
     1381 ttatgccccc tgaccaaagg agaagacaaa tcaccgtccg gctcctcctg acaacttccc
     1441 gcatacacgc cgtgcgttgc ccctgtattc ggacgtactc tgtggcggcg ttcgtagatt
     1501 tccagatatt cggaacgtat cttttcatcg ccgtaggcat agccgacaaa gaaatcatgc
     1561 tcccgcccga acagcccata tgtgccggtc aggtcaagtt taattcccca ttggcggtcg
     1621 tctttggtat gccgcaacgg catataactg tatcgtcggt tggcggtagc cttccgatta
     1681 aaggaagagt catacaggct gtttggaaaa cgttgtgccg cattattaaa gatgccttcc
     1741 ttcgcaaggg ctttatcgac aaattccgac ttgtcggcat caacacccag atccccccaa
     1801 gaaccttgac agataaagtc cagcgcaaaa ggggcactca tacactcgtc aaaaccggct
     1861 ttgcgttctg cggcacggcg gctgcgatac tgttcgaaag cagtattatc gaaacggttt
     1921 ttaacaaaat cgtctttgcg ctcacggtat tccttggcgg tttcgtcacg atatgctttc
     1981 agtttctcca atgccttatc tttcggctcg aacgggatga cttcgttttt ttcggtcaaa
     2041 aagcctaccg catcctcatc tgacaaaccc gccgcatgtt cgtttttcag aaaaaactgc
     2101 cccaccttcg catcggattc attcttggta taagacactt cggcattgag ctgccaaccg
     2161 ttgtcaaaca catgtttgaa acctgagaaa aggttgtatt tgtcagcact caaccgcgac
     2221 caatcctccc ccaaataagt gttgcgcggc agttgcaaag gccggttgca ggcaggcgtt
     2281 gaactgaacg gggcagtttt ctgattttca caggacaaaa taatgcccga aaaatcagga
     2341 acctccctac tcttctgata catgccgccc aaagtaagca cactgctgtc gcccgcatcg
     2401 gcttcggcaa tgccgtaaac catatgtttc ctgccccaaa cgcggtcttt aaacgatttt
     2461 ttatactctt ccgcacccac caaccttccg cgtaaggtat tcgccttatt caggctgcct
     2521 gaaacatcca acactgcacg ccggctgccg cgatggtcgg cggtcagctc tccggtatgt
     2581 ttgaaagaag cggtaggtcg cttacggatc aaattgacgg ttccacccgg ctctgaattg
     2641 gattgggtca accccgttgc accccgtaca acttcaatat ggtcataaac cgccaaatcg
     2701 gtactcggag acacgtcgat tttcgccgta tatcccgaac ggcctgcaac attgacggta
     2761 ataccgtctt caccaatctg atcaatatag aaaccgcgtg acaaaaaccg cgtctgcaag
     2821 cctgaatcgc gcacaacgtt gacacccgtc gtgtttttca ttgcctcttc aagcgtatgc
     2881 accgccttat cgtcaaggcg gctgcgcgtg atgacgctga ccgactgcgg cgtatccttg
     2941 cccgcaatcc tcatacctgt ggcggtggac atccgatcta tcgtataaga acgggtcttc
     3001 tcggtcttgc ccgacagagc atgagagccg cgtacattga ccgtatccag actgacggta
     3061 ttgccgtctg aaacaggcac aacaccgtct gcaaaagaac caccgtaagc cgataacagc
     3121 ataacggtca gaattttaag tgaaaaatga ttttgattca tagagacctc tgtaatatgc
     3181 aagtgtgcaa atcgtccaaa ggccctcaca actgttttga ttttttatat taattgaaaa
     3241 aaagtaattc tcaattaaat ttatagatgg gattgtattc ccattttgac aaaaaacaaa
     3301 ctgctcctta ccgtttattt caaaaaacga taatattgta ttgaaaaata tccgaattta
     3361 aatacagatc gtcaatgcgg aaaaaaacac ccaaattggc tataatcccg acaaacacac
     3421 tcaaggacaa caacatggca gcctcgcccg aagcaaaatt cactgaagaa aagattttgt
     3481 gggtcaaaca ccacacgccg aaactcatca ctttcgccat cagccgtccc gaatcctacc
     3541 gttttaaagc cggacagttc tcccgactcg gtttctacga aggggaaggc tttatttggc
     3601 gtgcctactc catcgtttcc gcagaatatg cagacacgct cgaatatttt gccgtactca
     3661 tccaagacgg ccccatgtcg gcccgtttcg ccaaaatgca acagggcgac accatcctgc
     3721 tcgataaaaa tgccaccggc ttcctcctgc ccgaacgctt ccccgacggc aaggatttgg
     3781 tgatgctctg caccggctcc ggcatcgccc ccttcctttc cattctcgaa caacctgaaa
     3841 tccgacagcg tttcgatacc gtcaacctga tacattccgt atcttttccc gaagaattga
     3901 ttttcaacga ccggctcgcc gcattgtccg aacatcccct ggtaggcgaa tacggacact
     3961 cgttccattt cgtccctgtt accacccgtg ccgccaaccc ctcgggctta agcggaaaac
     4021 gcattccgga actcttaaaa aacagcagca tcgaacaggc gctgcatacc aagttcaccc
     4081 cggaatccac acggtttatg atttgcggca acccggaaat ggtcaaagat actttccaaa
     4141 cgctgcttga catgggttac gccatgcacc gcaaccgcat tcccggtcaa atcatgatgg
     4201 aaaacggctt ctaaaaacca ccctgcttgt ccgatgcctt cggatggacg gtcaaaccga
     4261 cacggcacga aaaccgcgtc ggcaaaaatg ccgtctggaa aaattcagac ggcatcttcg
     4321 gacacattac ctgcaaacgg caacacattc caacccaaac cgattaggca atcaatacga
     4381 acggctgttt acatacttac cggctttcat caaccggtat cgatttaacc gatttcctta
     4441 atatttttcc tgtccgtttt aaacttcgcc ttaaacgcat ccagtaaatc tttatcgaaa
     4501 taccaaaacc cgtcatccat ttccaatgcg ccgcccattc catgcagaac aactttttcc
     4561 cccgttaaag gatcggtttc ggtaatttcc acctcatgca tttttcccca atccaaaaca
     4621 ggcaacttgt gtgcaaacgc caaaacctgt tcgtcagaac ggccacacgc cttatcgcat
     4681 cggcctgaaa catatacggg acaaggctca tcctctgcat aaccgtccgg aaggataccg
     4741 gctgcggcag gacacaatcc gtatgtttcc gcccacaacg acaaagcccg tctcgctgcc
     4801 tttttattgg caaataatcc ggtaggcgga ttatccgtca caccgttttt caaagccgct
     4861 gttttcgcat tcaacatgcc gtctgaacct ttttcaaacc tgacggtcgt aaatgtttta
     4921 agcagatttt tggcagatac ataacaaccc gaatgataac gtccgaccaa ttccgcttta
     4981 atcttatatg catgcaggct gcccaatgca ggaaaaaaac ggacttcctc cgtattgcac
     5041 caatcaaacg gggcttttcc ggaatccaat aaagccgaaa cctcgctata tacccgttca
     5101 aacgtaccga tgtaatttac ttttcctccg ccgtcgaaac aggccagcac ccccataccg
     5161 tcaggcaaac cgtacaactg ttccctcaac cgttcgggca gcgcggcagg cagcggtttc
     5221 ggattcatca aacggaaaca ctgcctgatc catgcctcaa ccccgtgttc cgacagactg
     5281 tattccaaat aatcacacaa tgccgataca tccgccatcg cacgatgcct gtcttccaca
     5341 acaatcccca acctttcgat gatactgtcc aggctgtgct tgtaaaattg cggatacaga
     5401 caccgggaca gctgcacact gcacaaagca ggcgatgaaa atccgatacc cgcacgatga
     5461 aactcatgct ttaaaaacgt atagtcgaaa cggctgttat gtgcaaccag cacacaaccc
     5521 ttcaataccg aaaacaactc gccggcaatc tctgcaaaaa caggcgcatc ggcaaccatg
     5581 ccgtctgaaa tccccgtcag ccccgccaca aactgcggaa tcggtttttg aggattaacc
     5641 agccactcat gcctcaccac ccttccctgc tcaaacttga ccaaagccac ttcggttacc
     5701 ctgtcttcat acagattgcc gcccgtcgat tccaaatcga ccacggcaac cggcattcca
     5761 aaccgtaaaa acgccttttc cagcaagggc caacgggaag caacaatcat tttattctct
     5821 ttaaattcaa acaacaaacc gatattttac acttttaagg catttcatcc aacaaaacaa
     5881 ttgacagaat ccgatgatta ccctaaaatt caaatctttc ttgcagtgca cccgtagctc
     5941 agttggatag agtatctggc tacgaaccag agggtcgggc gttcgaatcg ctccgggtgc
     6001 gccagtaaga aaatacaata tgcgcccatc gtctagcggt taggacatcg ccctttcacg
     6061 gcggtaaccg gggttcgatt ccccgtgggc gtgccaaatt ccaaatcccc gagattatcc
     6121 ctcggggatt ttttattgcc tcggcaactc gttaccatat ctttacctac ccccttcatc
     6181 agaatctcag acgtaatcga atcatattca aacctttgcc gtgcaaaccg atatcccata
     6241 accggatgcg gtgtccgtcc aacattttac ccgattgaaa cgcctgatat attgcacccc
     6301 atcaacgtgg cattactttt cttaacaatc ccctttgaca gcaactgact agggcttttt
     6361 tatgccatca tcaaatttat agtggattaa ctttaaacca gtacggcgtt gcctcgcctt
     6421 gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact
     6481 ataatatttt ctctcccgat tgaaacaggc gtaacagaat atgcccgaag ctccggctgc
     6541 tttcttgttt accgccgcga tatttagagt ataataccaa atttgagcaa tggttctaaa
     6601 acagttagaa ccatttttca tgagcctgac tgattcgtac actcggagaa actgatgcag
     6661 aatatttttg accctttggt tattcgtgga aaatccctta cccccatcgt gcaaggcggt
     6721 atgggggtcg gtgtttccgc atcgggtttg tccagcgcgg tggcacgtga aaacggtatc
     6781 ggaacgattg ccagtgtgga tttgcgccac cttcacgaag acctactcgc cgaatcccaa
     6841 atcaatccga gtgaagagaa atatacgtct ttgaactgta ccgcattgga cagggaaatc
     6901 caaaaagcca aaagtgcttc agagggaaaa gggctgattg cggtcaacgt gatgaaggcg
     6961 gtcaaagacc acgccgcata tgtccgccag gcttgcgaat caggggcgga tgcggtcgtg
     7021 atgggcgcag gcctgccttt ggacctgccg gaaatgaccg agggctatca taaagatgtc
     7081 gcgctgctgc cgattctgtc cgaatcgcgc ggtattaata tcgtcttgaa acgttggatg
     7141 aaaaaaggca tattgcccga tgcgattgta gtcgaacatc ctgcccacgc ggccgggcat
     7201 ttgggcgcgt caaccgtcga aggcgtaaac gatgctaaat tcgacttcaa acgcgtgatt
     7261 gaggaaacgt ttgaagtttt caaaagttta gggctggaaa gcgaaaaaat cccgcttatt
     7321 ttggcaggcg gtatggcaaa ttttgaaaaa gtcaaaaccg ccctaaagaa ctggggagca
     7381 tccgccgttc aaatcggtac ggcttttgcc gttaccgaag aaggagatgc acaccttaac
     7441 ttcaaaaaaa cgctcgccgg tgcggaaact gaaaaagtag ttgaatttat gtctgttgcc
     7501 ggtttgccgg cgcgcggcgt acgtaccaaa ttcctagaca gctatatcag gcgcgaaggc
     7561 aaacttcagg caaacgccaa agccgacccg cgccgctgca cccaaggatt aaactgcctg
     7621 accagctgcg gcctgcgcga cgggctttcc aaagccgggc aattctgtat tgatatccag
     7681 cttgctgccg cattccgtgg agaagtggat aaaggcctgt tcttcagagg taaagacccg
     7741 ctgcccttcg gcaatgccat ccgcaccgtc cgcgagacga tacaatatct gctgacgggg
     7801 agcgaacctg ttgcaacgct cggacgctga catcaggtag gatttgattt gcaaacaaaa
     7861 tgccgtctga agggccttca gacggcgttt ttcaggctgc gttctggaat agtgttaaaa
     7921 aataaacggg atgagctgca tttattttcg tccgacaaat caaaccatca cgccgaatga
     7981 tcaaataatg cctgcacggc gttgcatctg gcaaatcaat gcaatgaaaa cacggctttt
     8041 ttatttgctt tcagtattat tgaaaagttt gtccatcggg gtcaaatcga ccgcattgcc
     8101 ttggctggta atccatttgt tctcgacgcg ccacacgcct gccacgtcct ccacgcgcac
     8161 ataccagtgg ttggtcggcg aaagggtttt gaacaccgcc tcatattccg ccctgccgtt
     8221 ctgcgcgctg ccgacgggct tgagggcgac agtttggtcg tccgccttgc gggtcgggtg
     8281 catcagcagc aggttcaaag gctgtttgcc gtcaaactcg ccgccgacaa acacttttgc
     8341 cgcattcata tcgggggaaa tgagaacctg caccccgata tgccgtctga cggcttcttc
     8401 atcccgatga agctggatgt cgatatgttt gccgtcttta taataatcgt ccgtaaccaa
     8461 atctgtcgcg tgctgctgcg cgacaaaaaa catagcgacg ctggcgatga cgacaaaaat
     8521 cggccccgcc atcaagatcc acggccagac gtgtttgtac caaggtttga ttggagtgtt
     8581 ttgagacacg gttattctcc gataaaggtt gcatcttctt ccaagacgac cggcttgccg
     8641 tcgggcgcgc cgctttcgcg gtattggaag gtaaattcga tagggtggct gcctttgtcc
     8701 gcgtattccg gaatggtgga cacttggacg ggaagggtta ccgtttcgcg cggggcaacc
     8761 ttgataccgc cttcgggcag cccggtcagg gcgatttcgt caaagccttt gacacttgcg
     8821 gtaatgagct gttctttttc acttttgttg atgatacgca ggctgtatgc gttttccagc
     8881 cagcctttgg cgttttcgcg caccagtacg ccacggtctt tcaaaatatc gacctcgacc
     8941 attttgcgcg tggacaaacc gaccaaaaag gcggcgataa ccactgccaa caccgcgccg
     9001 taacctgcca cgcgcggtct gagcagccgt tttttaatgt ctttttcaga atattcgtgt
     9061 tccaacgcgc tttcggtcgt ataacggatt aatccgcgcg gatagcccat tttgtccata
     9121 atctcatcac acgcgtcgat acaggcggcg cagccgatac attggtactg cagaccgttg
     9181 cggatgtcga tgccgacagg gcagacttgg acgcacatcg cacagttgat gcagtcgccc
     9241 aaacccgcct cttccttatt gaccgttttc ttgcgcgcgc cgcgcggttc gccgcgttcc
     9301 gcgtcataag aaacaatcag cgtgtccttg tcgaacatcg cgctttggaa acgtgcatac
     9361 ggacacatat gcaggcatac tttttcgcgc ataatgtggg cgaagaagaa ggtcataaag
     9421 ccgtaaaacg ctgtggcaaa catcgcgccg ccgcctgctg ctccagtgaa taaatcggga
     9481 acgaactggc ggatagggac aaaccagcct gcaaacgtga tgcccgtcca cgcgcagaca
     9541 aggaaaatca acaggtattt ggtggctttg atgcggattt tagtgaaatt ccacggcgat
     9601 ttttccagtt tcagccgttt gtttctatcg ccttcgacca ggttgtcgat ccacagcata
     9661 atttcggtgt aaaccgtttg cgggcaggaa tagccgcacc acagtcgccc ggcaatcgtc
     9721 gtccaccaaa acagcccgaa ggcgcaaatc atcagcagca aggcaaggta aatcaaatcg
     9781 cccaccccca acgacaatcc gaaaatgaag aaatgccgtt cggggatatt gaaaacgaca
     9841 gcctgcctgc cgctccagtt gaaccacgga atgacgtaaa acacaaactg cgtcgccaat
     9901 acggcggcga tacgcagttt ggcgaaccgt ccttccgcct ttttcggatg gatgcgttcg
     9961 ccttcgggat ggatttgaat cacgctggct cgcggatcga acgttttttt tgctttcggc
    10021 gcggcttttg tttgttcgga cgtgccgatt ccggatgccg gactgccggc ttggttttcc
    10081 gtggtcattc tgcattcctt agatttttga ttgatggttt gccctttacc gccgccgttt
    10141 gcttttcaga cgtcattttt cttgtttttt aaggcgttgt gtttcaagtt ttgagaaaat
    10201 ccgtttttcc caaaatatat ttccgctatt gtacaacttt atgcgccgtc cggatgtatg
    10261 gggcggatac atttcccatc cgcatcaaaa cgcctggatt ttaccttacc gcccgaacaa
    10321 aatccgaata cggttaaaaa aaaagactaa aaaaaaaccg acacccccat atcggcagaa
    10381 ccgacggcgc aagctcataa acaaacgcta tcgacaatcc ggcacacaat ctataacttt
    10441 ttatttcaaa aggaataatg gcaggcttcg cccgcaaatc gaaaatcctt ccccaccggt
    10501 ctcctgccgc cgccttcccc cgcgtccgcc cttttcttga aagcataagc gaatcgggcg
    10561 ataatcaacg ccttccgatt atccactcat ccgaaacacc agcaaggaaa atacaaaatg
    10621 tctcaactgg caaacgcaat ccgcttcctc tcggcagatg ccgttcaaaa agccaattcc
    10681 ggccaccccg gcgcgcctat gggtatggcg gaaatggcgg aaacattgtg gacgaaattc
    10741 ctcaatcaca accccgccaa ccccaaattc tataaccgcg accgcttcat cctctccaac
    10801 ggccacgcgt ctatgctgtt gtacagcctg ctgcacctga ccggctacaa tctgggcatt
    10861 gaagacctga aaaacttccg ccagctgcac agcaaaaccc ccggccaccc cgaatacggc
    10921 tacaccgacg gcgtggaaac cacgaccggc ccgttggggc aagggattgc caacgcggtg
    10981 ggtatggcat tggcagaaaa aatccttgcc gccgaattta ataaagacgg tttgaacatc
    11041 gtcgatcatt acacctacgt ctttatgggc gacggctgtc tgatggaagg cgtatcgcac
    11101 gaagcctgtt cgctcgccgg caccttgggc ttgggcaaac tgattgtttt gtatgatgac
    11161 aacaatattt ccattgatgg taaagtggac ggctggttta ccgaaaacat cccgcaacgc
    11221 tttgaaagct acggctggca cgtcgttcct aacgtaaacg gtcatgacac cgccgccatt
    11281 caagccgcca tcgaagccgc acgtgccgaa accggcaaac cgtccatcat ctgctgtaaa
    11341 accttaatcg gcaaaggcag tgccaataaa gaaggcagcc acaaaaccca cggcgcacct
    11401 ttgggcgcgg acgaaatcga agccacgcgc aaacatttgg gctggactta ccctgccttt
    11461 gaaatcccgc aagaaattta cgatgcgtgg aatgccaaag aaaaaggcgc gaaactggaa
    11521 gccgaatggg acaaactgtt cgcgcaatat caagccaaat atcctgccga agccgcagaa
    11581 tttgtgcgcc gtatggataa aaaactgccg gaaaactttg acgagtacgt tcagacggca
    11641 ttgaaagaag tgtgcgccaa agccgaaacc atcgctaccc gcaaagccag ccaaaacagc
    11701 atcgaaatct tggcaaaaga gctgcccgaa ctggtgggcg gttctgccga cctgaccccg
    11761 tccaacctga ccgactggtc aaacagcgtt tccgttaccc gcgacaaagg cggcaactac
    11821 atccactacg gcgtgcgcga gttcggtatg ggcgcgatta tgaacggttt ggcattgcac
    11881 ggcggcgtaa aacccttcgg cgcgactttc ctgatgttca gcgaatacga gcgcaacgcc
    11941 ctgcgtatgg cggcactgat gaaaatcaac cctgtatttg tgttcaccca cgattccatc
    12001 ggtttgggcg aagacggccc gacccaccag ccgattgagc aaaccgccac cctgcgcctg
    12061 attccgaata tggacgtatg gcggccgtgc gacaccgccg aatccttggt cgcttgggca
    12121 gaagccgtca aagccgccga tcacccgtcc tgcctgattt tcagccgtca aaacctgaaa
    12181 ttccaagcgc gcagcgaaca acaactggac gacatcaaac gcggcggcta catcatcagc
    12241 gaagcccaag gcaacgccca agccgtcatc attgccaccg gctcagaagt cgagctggct
    12301 ttggaagcgc aaaaagccct cgccgcgcaa aacatcgccg tgcgcgtcgt ttccatgccg
    12361 tccaccaacg tattcgaccg ccaagacgcc gcctatcaag ccgccgtcct gcccgaaagc
    12421 ctgccgcgca tcgccgtaga agccggacac gccgacggct ggtacaaata cgtcggtctg
    12481 aacggcgcag tcgtcggcat caaccgcttc ggcgaatccg cccctgccga tttactcttc
    12541 aaagcattcg gctttaccgt ggacaatgtg gtcgatacag tgaaatccgt gctgtaaacc
    12601 gcagccgccg catcaaatgc cgtctgaacg cttttcagac ggcatttttt ttgccctcca
    12661 gccttaatgc ttcctcattc cgccaatctc cattataata tttgcgaatc actcttattc
    12721 acatttcaaa aggagaaacg catgagcacc cgtaccgaac acgacaccat gggcaatgtc
    12781 gaagtcccgt ccgaagccta ttggggcgcg cagacccagc gcagccgcaa caatttcaaa
    12841 atcggcggcg aaaccctgcc gcagccgctg atttatgctt tggcgctggt gaaaaaagcc
    12901 gccgccgcga ccaatgtttc cctcggcagg attaagccgg aacaggcgga tttgattacg
    12961 caggcggcgg atgatgtgtt gaacggcagg cttgacggac agtttccttt agtggtttgg
    13021 cagaccggct ccggcacgca gtccaatatg aacatgaacg aagtgctggc aaaccgcgcc
    13081 aacgaaatcg ccggtacggg tttggcggct tatcagcccg tccatcccaa cgaccatgtg
    13141 aaccacgcgc aatcgaccaa cgacgcattc ccgaccgcta tccacgttgc cgccgcgatt
    13201 gaaatcaacc gccacctcat ccccgccgta aaagccctgc gcgatacctt ggacaaaaaa
    13261 gcccaagctt tcgcccccat tgttaaaatc ggccgcaccc acttgcaaga cgcgacgccg
    13321 ctgactttgg gacaggaatt ttccggctac gtttcccagc ttgatcacgg cttaggccgt
    13381 ctgaacgatg cgcttaaaga cttgtatgaa cttgctttgg gcggtacggc ggtcggcacg
    13441 ggtttgaaca gccatcccga atacgccgaa aaagccgccg ccaaactcgc cgaactgtcc
    13501 ggcttgccgt ttgtcagcgc gccgaacaaa tttgaagcct taggcggacg cgatgccgcc
    13561 gttgccgctt cgggcgcatt gaaaacgctg gcggcaagcc tgaacaaaat cgccaacgac
    13621 atccgctggc tggcaagcgg cccgcgttgc ggtttgggcg aaatcaaaat ccccgaaaac
    13681 gagccgggtt cgtccatcat gccgggcaaa gtcaacccga cccaatgcga agcaatgacg
    13741 atggtgtgct gccaagtgtt cggcaacgac gttaccatcg gtatggcggg cgcgtcgggc
    13801 aatttcgagc tgaacgtcta tatgcccgtt atcgcctaca acctcttgca atccatccgc
    13861 ctgttgggcg acgcgtgcaa cagcttcaac gaacactgcg ccgtcggcat cgaacccgta
    13921 ccggaaaaaa tcgactattt cctgcaccat tccctgatgc tcgttaccgc gttgaaccgc
    13981 aaaatcggtt acgaaaacgc cgccaaagtc gccaaaaccg cctacaaaaa caacaaatcg
    14041 ttgcgcgaaa ccgccgttga gttgggcttg ctgacgggcg aagaatttga cgaactggtc
    14101 gttcctgccg atatggttca tccgcgctaa tctttccctc aaataaaatg ccgtctgaaa
    14161 cctcgttcgg acggcatttt ccgttgcctg caaactagcg gcgtttgaac agcctgtccc
    14221 ccaccgccgc tgtaaccgca cccccgacca cgaccagtgc gccggcataa cccaaaccgt
    14281 tcatatccgg cgcggcaaaa gtatcaggca tcacataatg cccgagcaaa gaaaatatta
    14341 cggtaaacac ggggagcaag gttgttaccg cgctgacttt ggaagcctcc caatgtttca
    14401 acgcctcgcc gaacgagccg taaccgatta acgtattcaa gcagcaatac gcaaaacaaa
    14461 cccacgccaa cgtaccgtcc aaacttccga tgtgtgccgg ttcggcaaac ggcaggaaca
    14521 cggcggcact tgccgcataa atcaacagca gaatctgttg cggcccgaat tgcgccgaca
    14581 gcagcttttg cgccacggca taacacaccc atgccatact gcctgccgca cacagcaaca
    14641 cgcccttcgc atacgcgccc aaacccgaca actcgccgaa tttatcgtta aaaaacataa
    14701 gcaaaccggc aagcagcaaa accaagccga ttttctgagc ggcagtcatc cggtctttaa
    14761 acaccaacac accgacaaca atcatcgtaa acggcgaaat ctgccacaaa acctgcgtcg
    14821 tggtcggcga aatataatgc agcccttggg caatcaacac aaagtttgcc gaaatgcccg
    14881 ccacgccgag cagcagcagc ctgaatgagc accaagaaaa atcccgccgc ttcggcagcc
    14941 gcccgcccaa tgccagcaaa acaaacaata ccgccgccgc cacggtaaaa cgcacccaca
    15001 ccagcgtcgg cgcatcgaca aacttcaata cctgccgcac ggcaatcggc agcgttcccc
    15061 acgtcatcgc cgccaaaagt gccaacgcga agcctaggag cggcctttgg ttttccatcc
    15121 tgattttcct attttttaaa caaccgtatt gccggacgat gccggtttgc cgcatcgggc
    15181 aatgatggtt caagcgtttg gcgtttgatt ccaacccttt gatttcaaac aaaccggctg
    15241 aagcccggct attgcttcgc gctatttgaa aacaccgcct gaattt
//
LOCUS       DAXHSJ010000035        14533 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000035 DAXHSJ010000000
VERSION     DAXHSJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.035"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(105..389)
                     /locus_tag="ACFM8U_000784"
     CDS             complement(105..389)
                     /locus_tag="ACFM8U_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6334052.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTLRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
     gene            complement(862..2127)
                     /gene="ftsY"
                     /locus_tag="ACFM8U_000785"
     CDS             complement(862..2127)
                     /gene="ftsY"
                     /locus_tag="ACFM8U_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC6334053.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            2273..3841
                     /gene="msrAB"
                     /locus_tag="ACFM8U_000786"
     CDS             2273..3841
                     /gene="msrAB"
                     /locus_tag="ACFM8U_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC6334054.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            complement(3943..4428)
                     /locus_tag="ACFM8U_000787"
     CDS             complement(3943..4428)
                     /locus_tag="ACFM8U_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC6334055.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(4455..5303)
                     /locus_tag="ACFM8U_000788"
     CDS             complement(4455..5303)
                     /locus_tag="ACFM8U_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC6334056.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            complement(5397..6398)
                     /locus_tag="ACFM8U_000789"
     CDS             complement(5397..6398)
                     /locus_tag="ACFM8U_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6334057.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            6457..7137
                     /locus_tag="ACFM8U_000790"
     CDS             6457..7137
                     /locus_tag="ACFM8U_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC6334058.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFAALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKAFGMKTVWFGAKSHALPFIDTCARDMAQLARYAETLSER
                     RQNHYNTP"
     gene            7163..7435
                     /locus_tag="ACFM8U_000791"
     CDS             7163..7435
                     /locus_tag="ACFM8U_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC6334059.1"
                     /translation="MRKTFLILTAAAALLSGCAWETYQDGNGKTAVRQKYPVGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPDQTGNNADEESRQHWQKPKFQNR"
     gene            7490..8860
                     /gene="glmU"
                     /locus_tag="ACFM8U_000792"
     CDS             7490..8860
                     /gene="glmU"
                     /locus_tag="ACFM8U_000792"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC6334060.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLSAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGNGSVTAIVEEKDANTA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKRQLAELERIFQTGQAQELLKAGVTLHDPARFDLRGRLKHGQDVVIDVN
                     CIFEGDIELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     ARLSDDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            8936..9265
                     /locus_tag="ACFM8U_000793"
     CDS             8936..9265
                     /locus_tag="ACFM8U_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC6334061.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(9337..10602)
                     /gene="efeB"
                     /locus_tag="ACFM8U_000794"
     CDS             complement(9337..10602)
                     /gene="efeB"
                     /locus_tag="ACFM8U_000794"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC6334062.1"
                     /translation="MSKKQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKIWCDGDLSLQICAFTPETCQAALRDIIKHTAQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGDITPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(10866..12032)
                     /gene="efeO"
                     /locus_tag="ACFM8U_000795"
     CDS             complement(10866..12032)
                     /gene="efeO"
                     /locus_tag="ACFM8U_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC6334063.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDQYSHTDLSDFQANVDGSKKIVDLFRPLIETKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            complement(12029..12868)
                     /gene="efeU"
                     /locus_tag="ACFM8U_000796"
     CDS             complement(12029..12868)
                     /gene="efeU"
                     /locus_tag="ACFM8U_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFC6334064.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMVLWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            13090..14415
                     /locus_tag="ACFM8U_000797"
     CDS             13090..14415
                     /locus_tag="ACFM8U_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC6334065.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
BASE COUNT         3364 a         4357 c         3675 g         3137 t
ORIGIN      
        1 gattcccgcc tgcgcgggaa tgacggttta gaagttgccc gaaacctcaa aaaaaaaaac
       61 cgaaaccgaa caagccggat tcccgcctgc gcgggaatga cgggctaaat aatatcaaac
      121 cataaatcct gccaagaaac attattttct tcaatcagtt gcaatttcca agccctgttc
      181 catttcttca actgtttttc ccgagtaatt gcactctcca tcgtaggatg cagttcatac
      241 caaaccagca tagtaacgtt gtaccgtaat gtaaatccct caatcaaatg ctccctatgt
      301 tggtaaatac gttgcaccaa atcagatgta acgccaatgt ataacgtgcc attacgttgg
      361 cttgctaaaa tataaaccgc aggctgcata taacaccctt ttgaattatt taaatttata
      421 ttcccgcgaa caccatcccg tgattacttt aacccttcgt tattcccata gctttccatc
      481 attcccgcaa ctcttcgtca ttcccgcgaa agtgggaatc tagaacgcaa aatctaaaga
      541 aaccgtttta cccgataagt ttccgcaccg acaaacctag attcccgcct acgcgggaat
      601 gacggcggag cggtttctgt tttttccgat aaatgccgca atctcaaatc ccgtcattcc
      661 cacgaaagtg ggaatctaga atctcggact ttcagataat ctttgaatat tgccgctgtc
      721 caatggtcta gattcccgcc tgcgcgggaa tgacggcatc ggtctgccgt tacaacacgg
      781 tttctttaga ttttacgttc tggattcccg cctgcgcggg aatgacggca tcggtctgct
      841 gttttcggac ggcatttcgg ctcaatccag cagtgcgtcc acaaacgcgc gcgcgtcaaa
      901 cgggcgcaaa tcgtctatgc cttcgcccac gccgatatag cggacgggga cggggcggtc
      961 ggaggcaagc gcggcgagga tgccgccttt tgccgtgccg tctagcttgg taacaattaa
     1021 accggtcagc cccaatgcgt cgtcaaaggc tttgacttgg ttgacggcgt tttgaccgat
     1081 attggcatca agcacgacga tgatttcgtg tggcgcgtcg ggcatggctt tttgcagcac
     1141 gcgtttcact tttttgattt cttccatcaa gtgaagctgc gtgggcaggc ggccggcggt
     1201 gtcggccagc acaatgtcga tgccgcgcgc tttggcggct tggacggcat cgaagcacac
     1261 ggcggcggaa tcgcccgtgg tttgcgaaat cacggttacg ttgttgcgtt cgccccaagc
     1321 ttgaagctgc tcacgcgcgg cggcgcggaa agtatcgccg gcggcaagca gcacagattt
     1381 gccctgcgct tggaaatatt tggcgagttt gccgatggat gtggttttgc ccgcgccgtt
     1441 gatgccagca agcatgatga caaacggctc tttggtttcg ggcaaaacca gcggtttctc
     1501 tagaggctta atcaggtcgt acaaggcttc tttcagcgcg ccgcgcaatt cgttgccgtc
     1561 tttcagccct ttgaggctga cgcggtcgcg cacgtctttc atcaggtatt cggtggcttc
     1621 catgcccata tcgctggtaa tcagcacggt ttccagctct tcgtacaaat cttcgtcgat
     1681 ttgtccgccg ccgaacacgc ccgccagcga tttcgccatt ttgtcgcgcg atttggtcaa
     1741 accttgtttc aaacgcgccg cccaaccgag cttgtgttct tcagttgtcg caacggcttc
     1801 ttgaacttgc ccgacagcct cgccgacggt ttcggcaacg gcttcttttg ccgcttcgac
     1861 ttgttccgct gctttttccg ccgcttcctc tgcttcagac agcatctcgg caacggtttc
     1921 ctttacctgt tcaaccgcac cgctgacggt ttcaacagcc gattcgacct gccctttgac
     1981 gctttccgtt aaagattcga cttcttcttt aatattttca actatttgag taacttcgga
     2041 ttctgctttt gctgcagttt cctgaacttg agcctcctcg ggagccggcg tttcctgttt
     2101 tttcttgcga cggaagaagc tgaacattga attttccttt taattttaga aacttgaaat
     2161 agggtgtatt gtagcgtatt ttgcgcggca aggttgtctg aaaatccggg ctgtaaggtt
     2221 tcggcatctc aaacgtctaa tcatgcaaac cgtccacaca ggaaacatta aaatgaaaca
     2281 ccgtactttc ttttcccttt gcgccaagtt cggctgcctg cttgcgctgg gtgcttgttc
     2341 gcccaaaatc gtcgatgccg gagccgcgac cgtgccgcac actttatcca ctttgaaaac
     2401 cgcagacaac cgccccgcca gtgtttactt gaaaaaagac aaaccgacgc tgattaaatt
     2461 ttgggcaagc tggtgtcctt tgtgtctgtc cgaattggga cagaccgaaa aatgggcgca
     2521 agatgcaaaa ttcagctccg ccaacctgat taccgtcgcg tctccgggct ttttgcacga
     2581 gaaaaaagac ggcgacttcc aaaaatggta tgccggtttg aattatccca agctgcccgt
     2641 cgtaaccgac aacggcggca cgatcgccca aagcctgaat atcagcgttt acccttcgtg
     2701 ggcgttaatc ggtaaagacg gcgacgtgca gcgcatcgtc aaaggcagca tcaacgaagc
     2761 gcaggcgttg gcgttaatcc gcgacccgaa tgccgatttg ggcagtttga aacattcgtt
     2821 ctacaaaccc gacactcaga aaaaggattc aaaaatcatg aacacgcgca ccatttacct
     2881 cgccggcggc tgcttctggg gcttggaagc ctatttccaa cgcatcgacg gcgtggttga
     2941 cgcggtatcc ggctacgcca acggcaacac gaaaaatccg agctatgaag acgtgtccta
     3001 ccgccatacg ggccacgccg aaaccgttaa agtgacctac gatgccgaca aactcagcct
     3061 agacgacatc ctgcaatatt tcttccgcgt cgttgatccg accagcctca acaaacaggg
     3121 caacgacacc ggtacgcaat accgcagcgg cgtgtactac accgaccccg ccgaaaaagc
     3181 cgtcatcgcc gccgccctca aacgcgagca gcaaaaatac caactgcccc tcgttgttga
     3241 aaacgagccg ctgaaaaact tctacgatgc cgaggaatac catcaggact acctgattaa
     3301 aaaccccaac ggctactgcc acatcgatat ccgcaaagcc gacgaaccgc tgccgggcaa
     3361 aaccaagacc gccccgcaag gcaaaggctt cgacgcggca acgtataaaa aaccgagtga
     3421 cgccgaactc aaacgcaccc tgaccgaaga gcaataccaa gttacccaaa acagcgcgac
     3481 cgaatatgcc ttcagccacg aatacgacca tttgttcaaa cccggcattt atgtggacgt
     3541 tgtcagcggc gaacctttgt tcagctccgc cgacaaatat gattccggct gcggctggcc
     3601 gagcttcacg cgcccgattg atgcaaaatc cgttaccgaa cacgatgatt tcagctacaa
     3661 catgcgccgc accgaagtgc gcagccacgc cgccgattcg catttgggac acgtcttccc
     3721 cgacggcccg cgcgacaaag gcggactgcg ctactgcatc aacggcgcga gcttgaaatt
     3781 catcccgctg gaacaaatgg acgcggcagg ctacggcgcg ttgaagagta aagtgaaata
     3841 agccgcaccg ccgcctaacc cgacaaaata ccgtctgaaa cctgcaacgt ttcagacggt
     3901 attttttatc cggcggggat tctgtccaga cggagatttt gtttagacag catcgccgcc
     3961 gttttcaatc agccccgcca accgttccaa cgcgaaggcg accgcctgcg cgcggacgga
     4021 ttcgcggttg ccgtcaaaac ggcgcattgc ttcgcaactt ccgcccggaa aggcaaaccc
     4081 gaaccaaacc gtgccgacgg gtttgctttc gctgccgccg cccggtccgg cgatgccgga
     4141 aataccgacg gcgtaatccg cctgcgccac ggctttcgcg ccgcgcgcca tctcatagac
     4201 ggtttggcgg ctgaccgcgc cgtgttcgag cagggtttcg ggcaacacgc ccaagcggtc
     4261 ttctttggct ttgttgctgt atgttacaaa actctggtcg aaccattgcg aactgcctgc
     4321 aacgcttgtg aatgcggcgg caagcattcc gcccgtacag gactcggcac agcttacggt
     4381 ttgacgtttt ttcgtcaggt ttcgggcgat ggtgtgcagc gcgtccattt tcccactccc
     4441 ctttcagacg gcgtttaaga attgatgatg tgtatgtcgc gttgcgggaa cgggatgttg
     4501 atattgactt tgcggaggtt ttcgaccact tgttcgttca aatcgcattg cagcgtccag
     4561 cggtctgctt cgtttgccca agcccataat gtgatttcga tggcattgtc gcccaaggcg
     4621 gtgatgtagg cggcggcctg ccgctcttcg ttttgaacgc tcaaggggtg ttcgacggcg
     4681 gctttcaaca ccgcctcttt cgccactttc aaatcgcagt tgtaatcgac gccgactatc
     4741 acttgggcgc ggcaaagcgg cagggtggaa cggttgacga tgctgttgcc catcaccacg
     4801 ctgttgggca gcacgacttc ttcgttgtcg gtcgtccgca aagaagtctg caccatttta
     4861 atctctcgga catatccttc aaaaccgccg acgcggataa aatcgccgac tttgaacggg
     4921 cgaaacagga taatcagcgc accggcggca aaattggaca gctggtcttt caaggacaac
     4981 gccaccgcca aacccgcgcc gccgattaag gcggttacgg atgttgtgga aacgcccaat
     5041 ctgcccaatg cggcaataat caccaaaatc aataagccga tattggcaac attacacaaa
     5101 aaactaatca gcgtggcatc gacctgcgcg cgcgtcatcg ccgccctcat cacggcgaca
     5161 atgcgtttcg ccgcccattt tccgaccaaa aaaataagca gcgcggcggc aaggttcagc
     5221 ccgaacgccc acgccttttc agccagatgc tcccaaccgg aaacactgat caggtgtaaa
     5281 aaatcaaatt gtttgaagtc cattgttttc ctcttgatcg aacatccgcc ccgtgcggcg
     5341 taatcggcac aggtgtaaaa atgccgtatg aagccctgcg gggcggtatg tttgttttat
     5401 ttcaaaaccg ttctaatcca acgggaaacc catccgcgct ggttcgcatc aatatcgcgc
     5461 tgcgcggggg cggtggtgtg cgtcggggct tgggcgaagc ggaacacgtc gggcaggcgc
     5521 gtgtttttga cggcggggta aacccacatt tcggacggaa ccgcctgctg cacttcccga
     5581 ctttgcagcc attgcaccag ttttgccgcc agcttcggct gtttcgcgcc cttcaagact
     5641 gccgcgcctt cgacctggcg gaatacgccg ccttttaaaa acaggttgcc cgtcggcggc
     5701 tcgctgtatt tgcctttgga aaaatacact tccgccgccg ggctggcggc ataaccgacc
     5761 accagcggat acgcgccgcc gttgtgcgaa aagtcggtgt aatacgcctc gctccagcct
     5821 ttggcgacct tcacgccgtt ctgccgcatc tgtgcccacc atttgaacgc gccttcttcg
     5881 cccagaccgc tgatgttcgc catcaggaag cccagccccg gggacgacgt ggcgggagac
     5941 ggcacgacca ataggttttt atattcgggg cgggtcaaat cctgcagggt ttgcggcagg
     6001 ggcagctttt tgccttcaaa ccattttttg tcgtaattga tggacacata gccgtaatcg
     6061 accgccaaag ccgaaggcag cccgaccgcg acgggggcgg attcgggttg cgctgccgcc
     6121 aaaatgccca tttcccgcgc cttgccgata ttggcgttgt ccaaaccgta caccgcgtcg
     6181 gcaatcgggt tggcgcggct caaaatcagt ttgttgagca tttcgttcgc gccgcccgcc
     6241 tgaataatcg acaccttcgc atcgtttgcc cgctcgaagc gcgcaatcag ccctttgggc
     6301 aggctgaacg acttatgcac cgccagcctg acttccgtct gcgcctgcag gtatgccgaa
     6361 accgccagca gcggcagcag ccaaattttc cgtttcattc cgagtcctcc tctattcgct
     6421 gtaaaataac attctaacaa attttcacgg ttcaacatgc aagaaaaccc gaccgtgtgg
     6481 ctgttcgacc tcgacaacac gctgcacaat gccgatgccg gcatcttcac actcatcaac
     6541 cgcgccatga cacgctatat ggcacgccgc ctcaaactct ccgaatctgc cgcgtccgac
     6601 ctgcgtcaag actattggca ccgctacggc gcaacgctcg ccggactgca aatccaccat
     6661 cccgaaatcg acatcgccga atttttgcgc gaaagccatc cgatcgatgc aatcctgact
     6721 aggctgcacg gcatgcctga aacacaaaac accctgagcc gcctaaaagg gcgcaaggcg
     6781 gttttttcca acggcccgtc gttttacgtc cgtgccgttg tcggcgcact cggtttggaa
     6841 aaccgtttcg ccgcactgtt cggcacggat gatttcggac tgctgtacaa acccaatccg
     6901 caagcgtatc tcaatgtctg ccgcctgttg gacgtaccgc ccgaatgctg cattatggtg
     6961 gacgacagcg cggacaacct gcatcaggca aaagcatttg gcatgaaaac cgtctggttc
     7021 ggtgcaaaat cccacgcgct gccctttatc gacacctgcg cacgcgatat ggcgcaactg
     7081 gctcggtatg cagaaacttt gtcagaacgc cgccaaaatc attacaatac cccctaaccc
     7141 ccgaaaatac gaaagaaaag ccatgcgtaa aaccttcctt atcctgaccg ctgccgccgc
     7201 ccttttgtcg ggctgcgcgt gggaaactta tcaagacggc aacggcaaaa ccgccgtccg
     7261 tcaaaaatat cccgtcggca cgcccgttta ttaccaagac ggcagctact cgaaaaatat
     7321 gaactacaac caataccgcc ccgaacgcca tgccgtgctg cccgaccaaa ccggcaacaa
     7381 cgccgacgaa gagtcccgcc aacactggca aaaaccaaag tttcaaaacc gataaaccta
     7441 ccctatgccg tctgaagccg cttcagacgg cattgcacag gaaaccgtca tgccgcaaaa
     7501 cactttaaac atcgtcatcc tcgccgccgg caaaggcacg cgcatgtatt ccaaaatgcc
     7561 aaaagtgctg caccgcatcg gcggcaagcc catgctcggg cgcgttatcg acaccgccgc
     7621 cgcgctgaat ccgcaaaaca tctgcgtcgt cgtcggccac ggcaaagagc aagttttaga
     7681 caccgtcaaa cgcgatgtcg tttgggtcga acaaaccgaa cagctcggca ccggccacgc
     7741 cgtcaaaacc gccctgcccc acctttccgc cgaaggccgc acgctggtgc tgtacggcga
     7801 cgttccttta attgacgttg aaaccctcga aaccctgctc gaagccgcag gcaacgaagt
     7861 cgggctgttg accgacgtgc ccgccgaccc gacaggattg ggccgcatca tccgcgacgg
     7921 caacggcagc gtaaccgcca tcgtcgaaga aaaagacgcc aataccgccc aaaaagccgt
     7981 gaaagaaacc aacacaggca ttctcgtcct gcccaacgcc aaactggaaa actggctgaa
     8041 cagcctttcc agcaacaatg cccaaggcga atactatctg accgacctca tcgccaaagc
     8101 cgttgccgac ggtattaaag ttcatcccgt ccaagtccgc gcctcccacc tcgccgccgg
     8161 cgtgaacaac aaacgccagc tcgccgaact cgaacgcatc ttccaaaccg gacaggcgca
     8221 agaattgctc aaagcaggcg ttaccctgca cgacccggca cgtttcgatt tacgaggccg
     8281 tctgaaacac gggcaagacg tcgtgattga tgtgaactgt atctttgaag gcgacatcga
     8341 gctcggcgac aacgtcgaaa tcggcgcaaa ctgcgtcatc aaaaacgcca aaatcggcgc
     8401 aaacagcaaa atcgccccct tctcccacct cgaaagctgc gaagtcggcg aaaacaaccg
     8461 aatcggcccg tacgcccgcc tgcgtccgca agcccgcctt tcagacgacg tacacgtcgg
     8521 caacttcgtc gaaatcaaaa acgccgccat cggcaaaggc accaaagcca accacctcac
     8581 ctacatcggc gatgccgaag tcggcagcaa aaccaacttc ggcgcgggta cgattattgc
     8641 caactacgac ggtgtgcaca aacacaaaac cgttatcggc gacgaagtcc gcatcggttc
     8701 aaactgcgtc ctagtcgccc ccgttaccct cggcaacaaa gtaaccacag gcgcaggcag
     8761 cacgattacc cgcaatatcg aagacaacaa actcgccctc gcccgcgccc gccaaaccgt
     8821 catcgaaggc tgggtgcgtc ccgaaaaaga caagcaataa agctatgccg tctgaagccg
     8881 gtttcaggct tcagacggca tcccaaaaac aaacatccga taaggacgga aaactatgtc
     8941 attaccccca tgcccacaat gcgcctccga atacacttat gaagacggcg gacaatacat
     9001 ctgccccgaa tgcgcccacg aatggaatga aaccgaatcc gccgccgacc ttgcggctca
     9061 agtgcgcgat gccaacggcg cggcgctgca aaacggcgat accgtcatcc tcatcaaaga
     9121 cctcaaggta aaaggcagct cgatggtgat caaacaaggt acaaaagtca aaggcatacg
     9181 cctgcaggaa ggcgatcaca acatcggctg caaaatcgac ggcagcgcga tgaatttaaa
     9241 atccgaattc gtcaaaaaag cctgaccgcc caaaaacaag aaacgccgtc cgaacccgtt
     9301 tggcaaggtt cggacggcgt tttttatatg gcggatttat acgcccagca gcccttgccc
     9361 caaaaagccg cctttttcca cgccgggcaa gacgaagaaa tagccgccgc cgaaggggct
     9421 gatgtattct tccagcggtt cgccgttaag gaggttttgc acgaagatga atccgtcggc
     9481 aaggtttgcc tgatagcaga cgaacaccag cccgacatca agctgtccgc ttgaggcaag
     9541 tccgcgcgaa tagctgtagg cgcggcggaa gaggcggtgt tttttgagga attcgggatc
     9601 gcgcggattc gccaggcgta tatggctgtc tttgggcgtg atatcaccct cggggtcttt
     9661 ggcaaaatcc ggttggtcgg cttctttttt gccgtccatc ggcgcaccgc tgtatttgcg
     9721 ccgcccgaaa atgtcggttt gctcttgaag cggcgtcctg tcccaaaact cgacaaagtg
     9781 gcggataagg cggactgcct gatagctgcc gtttttcgcc cactcaggtt cgtctaggct
     9841 gttggcggcc acacccgtcc acaaaacctc gtcggcggtt ttggggtcgg aaactttggg
     9901 gttgcctgtg ccgtccctga agcccaacag gttgcgcgcc gccatcgcgc cgggttcgga
     9961 tttgggctgc catccgtcga tactccagcg gataacggcg gtttgggcgg tgtgtttgat
    10021 gatgtcgcgc agggcggctt ggcaggtttc gggggtgaag gcacagattt gcaggctcaa
    10081 atcgccgtcg caccagattt tttgcagctt atcgttggag aagtcgcgca tttcctgcaa
    10141 atgaatcggt tttttgtctt tgagtccgaa ccggccgtca aacaggctgc tgcccacccc
    10201 cacggtaacg gtcaacccgt cggggttgaa ggctttgccc aaaatgccgc tgccggctgg
    10261 cggaagtttg tcgtcgccgt cttggtattc gccgccttgg gtgagaaact cgatgcgggc
    10321 ggtcagcgtg cggaacaggt tttccagctg cttggcactt tgcgcggtta cgtcgaaggc
    10381 gcacataatc gaaaacgcct gctgcggcgt aacgatgcct gcctgatgtt cgccgtagca
    10441 gggataggct tggggcgagt gttggctttc ggcggtgcgt tcggcggttt cgccctgttt
    10501 tttgccgccg agataacctc cgattgcgcc gactgctccg gctgcgatcg cggttttaaa
    10561 aagagtgcgc ctggtcggtt gtgcgggttg ttttttgctc atggtgtttc cttcaatatc
    10621 cgtgcagcag gatgggtatc cctgcacgcc gtttttccat taaccgatag cggcaggttg
    10681 aaatcctgcc ctgcaatata tggcggatat agtggattaa caaaaatcag gacaaggcga
    10741 cgaagccgca gacagtataa atagtacggc aaggcaaggc gaggcaatgc cgtactggtt
    10801 tttgttaatc cactataaaa cttcagacgg tatctgccgc caaataccgt ctgaacgctt
    10861 gcggcttatt tcaagccgag tatgccgcga agttgggcaa ggtcttcggc aagcgcgtta
    10921 atagaggcct gtaacgcttt gcggtcggct tcgcccagct tgtcgtaggt ttcaaaaccg
    10981 tctttagtcc ggtatttcgc cagaatttcg ttgacctgtt tgaagttggt atcggttttt
    11041 tccaacaagg ctttgttttt ggtctcgatc agcggacgga acaaatcgac gatttttttc
    11101 gatccgtcca cattggcttg gaagtcgctc aaatcggtgt ggctgtactg gtcttcttcg
    11161 ccgctgattt tactgcccgc cacttcttca atcagttcgg acgcgccgcc gaccaccttg
    11221 cccggaggaa acgccaatgc gtcgatttct ttttgcaggg cttcgacatc ggtcatcagt
    11281 ttcgctgcaa tttccttcac gccggacacg tctttttcca cccaaagggc gtattcgata
    11341 cggtggaagc cggtaaatcc ggcatctttc gcgccgtctt tgaagtcgtc ttcacgcgca
    11401 tcgatgacgg ggtcgagttc gctgaaaagc tcggcaatcg gttcgatgcg ttcgtaatgg
    11461 acgcgggtgt cggcaaacag ggatttcgcc ttttcaatgt cgcctgcttt gacggcttcg
    11521 gtaaaggttt tggttttcgc caccagctct ttaacctcgc cttgaacgta ggctttatag
    11581 tcggcgagcg gttgggacag tttttccaaa tccgcttcgt tggcggtgtc tttaaagccg
    11641 ctgtcggtta ccaccagctt gccgcgcgga ttggtcaaaa gaccgcaagt catttcgtat
    11701 tcgcccggca acagggtgac ggtcatttta tcggaaagtc cgggggcgat gttttcgcgc
    11761 tcgtccacca ccatcacgcc tttcaggatt tcccattcga gcttgcggcc gctgttgttt
    11821 ttaatattga acacaacctg tccgctcggc acggtcagtt ccatcggttc gcaggcattg
    11881 tcgttgacgg cgatactgac cgaaccgccc tcgttggcgg tttgcgcctc accggacgcg
    11941 gccggcgcag ctttctccgc ctccggcggc tggcacgcgg tcaaacctaa ggcaagcatc
    12001 acggacaatg cggtcaaatt gaattttctc atttcagctc ctctttacgg gttaaagttt
    12061 cagacggcct gctgccgcac aaaaaccaag tcatgacggg aataaggtac agcagccaaa
    12121 ccaaggcctc gccctgcgtc ggatggtcgg tatagccgaa aaatccgccg agcagcacac
    12181 ctaacggact gtcttcgtgc aaatattttg atgagtcgaa cacaatgtcc tgaagcgcgt
    12241 tccaaatgcc tgcttcgtgc agcgcgcgca gcgagccggc aagcaggccg gcggcaacga
    12301 caatcagaaa cgcccccgtc caacggaaaa acttcgccag attcaggcgc atcccaccct
    12361 gataaatcaa cgcgccaatc acggcggcag ccaaaacccc cgctaccgca ccggccggca
    12421 tctgccaagt cgggctctgt ttgaatacgg caagcagaaa aaaaacgctc tccaaacctt
    12481 cgcgcgccac ggcaagaaac gccataccga ccaaggccca tccttgtccg ctgccccggt
    12541 tcaaagccgc ctgcacagaa tcctgaagct gctgcttcat cgaacgcgcc gcctttttca
    12601 tccataaaac catataagtc agcatcgcga cggcaaccag gccgataatg ccgacgacga
    12661 actcctgctg cttttgggga atctcgcccg ttgccgaatg gatgccgtac cccagcccca
    12721 aacacatcaa agaagcaagg acgaccccga accagacctt aggcatcagt ttggaatgtc
    12781 cggactgttt cagaaaaccg gcaacgatgc cgacaatgag cgcggcttcg ataccctcgc
    12841 gcaacataat taaaaaagcg accagcataa acgcgaacga acaaggatga tgaataatat
    12901 attatcggaa tattttcatt gcttgtaaat acaaatgcaa gttattttta tctgcagtac
    12961 cgcgcggcgg aaagttccgc aggctgcagc tgcgccctgt gttaaaatcc cctctccacg
    13021 gctgccgcaa cgccgcccga aaccatcttt cttattactg ccttattact gccggcaaca
    13081 ttgtccatta tgaaaaaata cctattccgc gccgccctgt acggcatcgc cgccgccatc
    13141 ctcgccgcct gccaaagcaa gagcatccaa acctttccgc aacccgacac atccgtcatc
    13201 aacggcccgg accggccggt cggcatcccc gaccccgccg gaacgacggt cggcggcggc
    13261 ggggccgttt ataccgttgt accgcacctg tccctgcccc actgggcggc gcaggatttc
    13321 gccaaaagcc tgcaatcctt ccgcctcggc tgcgccaatt tgaaaaaccg ccaaggctgg
    13381 caggatgtgt gcgcccaagc ctttcaaacc cccgtccatt cctttcaggc aaaacagttt
    13441 tttgaacgct atttcacgcc gtggcaggtt gcaggcaacg gaagccttgc cggtacggtt
    13501 accggctatt acgaaccggt gctgaagggc gacgacaggc ggacggcaca agcccgcttc
    13561 ccgatttacg gtattcccga cgattttatc tccgtccccc tgcctgccgg tttgcggagc
    13621 ggaaaagccc ttgtccgcat caggcagacg gggaaaaaca gcggcacaat cgacaatgcc
    13681 ggcggcacgc acaccgccga cctctcccga ttccccatca ccgcgcgcac aacggcaatc
    13741 aaaggcaggt ttgaaggaag ccgcttcctc ccctaccaca cgcgcaacca aatcaacggc
    13801 ggcgcgcttg acggcaaagc accgatactc ggttacgccg aagaccccgt cgaactcttt
    13861 tttatgcaca tccaaggctc cggccgtctg aaaaccccgt ccggcaaata catccgcatc
    13921 ggttatgccg acaaaaacga gcatccgtat gtttccatcg gacgctatat ggcggataag
    13981 ggctacctca aactcggaca aacctcgatg cagggcatca aagcctatat gcggcaaaat
    14041 ccgcaacgcc tcgccgaagt tttgggtcaa aaccccagct atatcttttt ccgcgagctt
    14101 accggaagcg gcaatgacgg ccctgtcggc gcactgggca cgccgctgat gggggaatat
    14161 gccggcgcag tcgaccggca ctacattacc ttgggtgcgc ccttatttgt cgccaccgcc
    14221 catccggtta cccgcaaagc cctcaaccgc ctgattatgg cgcaggatac cggcagcgcg
    14281 attaaaggcg cggtgcgcgt ggattatttt tggggatacg gcgacgaagc cggcgaactt
    14341 gccggcaaac agaaaaccac gggttacgtc tggcagctcc tacccaacgg tatgaagccc
    14401 gaataccgcc cgtaaaccgg aaaacaatgc catctgaagc ccatccctag gcaatcgggc
    14461 gcattaaggc gaagttttgc gataccgcag gcacaagccg gccggtcgga caaaacgcct
    14521 ttcttatttg acg
//
LOCUS       DAXHSJ010000036        14160 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000036 DAXHSJ010000000
VERSION     DAXHSJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14160
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.036"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(242..1048)
                     /locus_tag="ACFM8U_000798"
     CDS             complement(242..1048)
                     /locus_tag="ACFM8U_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6334066.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(1049..1795)
                     /gene="pssA"
                     /locus_tag="ACFM8U_000799"
     CDS             complement(1049..1795)
                     /gene="pssA"
                     /locus_tag="ACFM8U_000799"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC6334067.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            2378..2860
                     /locus_tag="ACFM8U_000800"
     CDS             2378..2860
                     /locus_tag="ACFM8U_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC6334068.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGVMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(2912..4123)
                     /locus_tag="ACFM8U_000801"
     CDS             complement(2912..4123)
                     /locus_tag="ACFM8U_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC6334069.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            4346..6784
                     /locus_tag="ACFM8U_000802"
     CDS             4346..6784
                     /locus_tag="ACFM8U_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6334070.1"
                     /translation="MTEKSHKKTVKGRAGSPSPMSARNKKADNGARGNKVSERLKAVK
                     ELQKTEAKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMSDPSWSHSSPVAGDA
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKRTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGRSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNSKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAGAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHVGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            6992..8302
                     /gene="tig"
                     /locus_tag="ACFM8U_000803"
     CDS             6992..8302
                     /gene="tig"
                     /locus_tag="ACFM8U_000803"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC6334071.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADPSRLQAPTSLAVESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            8398..9012
                     /gene="clpP"
                     /locus_tag="ACFM8U_000804"
     CDS             8398..9012
                     /gene="clpP"
                     /locus_tag="ACFM8U_000804"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC6334072.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLQL"
     gene            complement(9065..10330)
                     /gene="ispG"
                     /locus_tag="ACFM8U_000805"
                     /gene_synonym="gcpE"
     CDS             complement(9065..10330)
                     /gene="ispG"
                     /locus_tag="ACFM8U_000805"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC6334073.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(10346..11107)
                     /gene="pilW"
                     /locus_tag="ACFM8U_000806"
     CDS             complement(10346..11107)
                     /gene="pilW"
                     /locus_tag="ACFM8U_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC6334074.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(11110..12204)
                     /gene="rlmN"
                     /locus_tag="ACFM8U_000807"
     CDS             complement(11110..12204)
                     /gene="rlmN"
                     /locus_tag="ACFM8U_000807"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC6334075.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTSAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(12347..12772)
                     /gene="ndk"
                     /locus_tag="ACFM8U_000808"
     CDS             complement(12347..12772)
                     /gene="ndk"
                     /locus_tag="ACFM8U_000808"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC6334076.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(12831..13982)
                     /gene="zapE"
                     /locus_tag="ACFM8U_000809"
     CDS             complement(12831..13982)
                     /gene="zapE"
                     /locus_tag="ACFM8U_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC6334077.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         3445 a         3483 c         3901 g         3331 t
ORIGIN      
        1 gtttgggagg cagccgcatc gcgtggaatc ggcacggcag gcgatgcgcc gatgccttcg
       61 ggcgtgaagg cggggcaatc ggcagtttgg aaaaaggcaa tggaatctgt gggcagaaga
      121 agttaagggt aaaagtcggg cgcacggtca ggttgtctgt ccttttgtcg gaacaggaga
      181 ctgggcagct ttgtcctgat taattgcttg tgcaaacggt aaaaaggttt tttcgtgtgt
      241 tttaaagcag gttgtacagc atttttccgg caatcaaaag cagcataatg ccgaaggatt
      301 ttttgagttt ggcagaagaa agtttgtggg cggttttgac accgagcggg gcaaaggcaa
      361 tggttgccgc gctgaggacg gcgacggcgg gcaggtaaag gaagcccagt gacccttcgg
      421 gcaatcctgc aatattcagg ccgttgagca gatacgatat tgcgccggag agtgcaatcg
      481 gccaggcaag gccggatgat gtgccgatgg ctttatgggc ggggaagccg cagtggatta
      541 agaaggggac ggaaagtgaa ccgccgccta tgccgaccca gctcgacatt gtgccgaaca
      601 gtgtggaaac cgcagtcagt ccgggcagtc cgggcagcgg gcgggatgcc gtctgagggt
      661 cggtatgcag tgttttgaat gcgacggcgg ttaaaaacag gatgaagaaa atttgaagcc
      721 cgaacgcggg gatatatttt gcggagagtg cgcccgtgaa tacgccgaat atcatacccg
      781 gcatcatcgt aaatacggtt ttccagtcga ccgcctgttt tttgtgctgc cccagcatac
      841 tggaaaaggc ggtgaagacc atgacggcga aggatgtgcc gacggcgagg tgttgcgcgt
      901 aaggatgttg tgccaaaccc tgcaaatcaa gcacccataa aacgacaggg acaatcagcg
      961 tgccgccgcc tacgccgaac aggccggcaa taaaacctgc cgcactgcct acggcaagca
     1021 ggattaagat aatgtcccaa tgccacattt aatccgcctt tctgtatttt ttccaaaatc
     1081 ggcgcgccgc cataatgtag ccggacaggc tgtatccgag aaagaacagg aagaggacga
     1141 gcgacggttc ccaagtgacc agaagcagca gtaagacggc aagcagcatt ccgacaaagg
     1201 ggacttgtct gcggatgttg atttctttaa aactccaaaa agggatttgg acaatcatcg
     1261 acaggccggc aaacagtgtg atgcccaatg cccaccagtg gacggcgggg aatttttcga
     1321 cgctgtggtt gacccaaatc agcccgacaa tcagcgcggc ggcagtcgga ctgggcacgc
     1381 cgataaacca gcgtttgtcc accttgccga tgagcgtgtt gaacagggcg aggcgcaggg
     1441 cggcgcaggc gcagtagatg aaggcgacgg aataaccgat tttgccgaac tgccaaagct
     1501 gccatttgta ggcaataagc gcgggggcaa cgccgaagct gaccatatcg gcaaggctgt
     1561 cgagctgttc cccgaacgcg ctttggctgt tggtcagccg cgccacgcgc ccgtccatac
     1621 cgtccagcag catagagatg aataccgcga tggcggcggt ttcgtaacgt ccgtgcatgg
     1681 attgggtgat tgcgtaaaac gcggaaaaca gcgcggcgat ggtaaaggag ttgggcagca
     1741 ggtagatact gttttgacgc agggagcgtt gcggggtgtt ggtcggggtt tccatagctg
     1801 aagtgtctca cggacgtgga gaatcgatag gggattataa cccaaaccat tatcgaacaa
     1861 tacgcccttt aggatgaagc cctcggaggc ggtttgtttt tctgatggcg gatgctttgc
     1921 ggggcggttt gactctgcct gccaccgggt gtgttgtttc agatttttgc cggatggata
     1981 ttgttgccgg gcatttctgg gcttgccgga aggtatcggg gggagggcat cggaatatgc
     2041 ggcgcgccgt tcaaaccggg gcggcgtttc cctattttca gggggttgca ggtttcaaca
     2101 aattgaaaga tattttttgc atttccgacc gtatgctgat tttgaaatgg gataaattat
     2161 aggacaaggt ttttcgcccg ccgcaaaggt tggccgcaat gccgtctgaa aacaacggct
     2221 tgccgctaag gcggcggtat gcggatttgc gctgccgaaa tcggtttgcc atacccggcg
     2281 gtgcaatctg cggggaggga taagccgata aggcgggact tatcctgttt tttgtttata
     2341 atgcaacaga atttggacga accgtcggga atttatcatg atgaaaaaga tactggccgt
     2401 atcggcacta tgcctgatga ctgcggcggc acgggctgcc gatacttacg gctatctcgc
     2461 cgtttggcag aatccgcaga atgcggacga tgttttgcag gttaaaacca caaaagaaga
     2521 ttcgacgaaa agcgaagcgt ttgccgagtt ggaagccttt tgcaaaggtc aggacacgct
     2581 tgcgggcatt gccgaagacg agccgactgg atgccggtcg gtcgtgtcgc tgaacaatac
     2641 ctgtgtcgcg ctggcatacc cgaaagcctt gggcgtgatg cgcgttgaaa acgccgtcgt
     2701 gattacttct ccgcgtttta cgagcgttca tcaggtcgca ctcaaccagt gcatcaaaaa
     2761 atacggcgta cagggacaat gcggcttgga aacagtgtat tgcacatctt cttcttatta
     2821 cggcggaact gtgcgctctt tgattcaaaa tctcaaataa aacggaaaat gccgtctgaa
     2881 agatgttcag acggcatttc tatatcgacg gtcaggattc tttcggatcg ggcagcaggc
     2941 tgttcaacat aatggcaagt acggcgcaca agcccacgcc ggcaaagctg aagctgccca
     3001 atttgagcgt catgccgccg atgcccgtgg tcagtaccga gctgacgatg accaggtttt
     3061 tcggcagcat caaatcgact ttggcatcaa tcagcgtttt cacgcccaaa gaagcaatcg
     3121 tgccgaacag cagcagcata atgccgccca ttactggcat cggaatggaa gccaaaaacg
     3181 cattgaattt gccgaaaaac gccatgcaga cggcaaaaac cgccgcccaa gtcatgatga
     3241 cggggttact gtttttggta atcatcaccg cacccgttac ttcgccgtag gtcgtaaccg
     3301 gcgggccgcc gatcagaccc gcaacgcaca cgcccaaacc gtcgcctgca agggttttgt
     3361 ccaagcccgg gtctttcgta tagtctttcc ccgtcacatt gccgattgcc atgatgccgc
     3421 cgatgtgttc gatggcgggg gcgacggcaa cgggcagcat aaacagtgca gcctgccagt
     3481 tgatctgagg cgtttcaaaa tggggaacgg cgaaccaggg cgcgtgtgca atgcttgccg
     3541 tgtccaccag tcccatcagc agtgccaaaa cataacccga agcgacaccg atcaagatgg
     3601 gaatcagctt catcatcctg ctgccgaaaa ccgatacgat ggcggtaacg gcaaaggtaa
     3661 agccggaaag aatcagcgaa tcggtatagt cgatgacctg tttgccgtcc gcctgaccca
     3721 ttgccatgct gcttgccgcc acggcaacag acagaccgat gaccatgatg acggggccga
     3781 tgacgaccgg aggcagcagt ttgtgtaccg ctgccagtcc gcgccaacgg atcagcgcgg
     3841 caaacacaaa atacataaag ccggcggcaa acagtccgaa catggtggaa ggcagccccc
     3901 attcgccgac ggagtagata atcggtgcga taaaggcaaa cgaagaacca agaaaaatcg
     3961 gcactttgcg tttggttgtg atttggaaca gcagcgttcc caagcctgcg cccaaaagcg
     4021 caagagccgg attcagaccg gtcagcaggg gaaccagcac cattgcgccg aatgccacaa
     4081 ataaaatctg tgcaccggaa acggcaagtt tcagttggtt cataccatcg tctttcagtc
     4141 ggttcaaacg gcacggatta tactggtttg ccgagggtct gcgtaaaaat acatacgtcg
     4201 ggcaacagat atgccgtctg aaaggcaacg tttgtctgtt gcaggatgaa agcaggagaa
     4261 atggagtata atccgacact ttccatattc aatccgcaag ccgtccggcc tccttatttc
     4321 agacggcatt ttccggtaat acggcatgac agaaaaatcc cataaaaaaa cagtaaaagg
     4381 cagggcggga agcccgtccc caatgtccgc acgcaacaag aaagccgaca acggcgcacg
     4441 gggcaataaa gtttccgagc ggctcaaggc ggtcaaagag ttgcagaaaa ccgaagccaa
     4501 aaaggcgcgt cccgaacatg tcgtcaacct tattggcgac gcgctgtggt tgatgggttt
     4561 ggcggcaacc ctgtatttgg cgatttccct gatcagtttc gatatgagcg atccgtcttg
     4621 gtcgcacagt tcgccggttg ccggagatgc cgccaattgg ggcggactgt tcggcgcgta
     4681 tgttgccgat gtcggctatt atcttttcgg ctggtcgttc tggtggtgga tagcggctgc
     4741 ctgcgtcatg ctgtataaaa atttccgtct gcacgcaaaa cggacggaaa acgaggcata
     4801 caaccacaaa atcgctgccg ccgcgctgtt tgtcctgacg gtcttcagcc ccgtcttgga
     4861 gtattttgtg ctgggcggaa aatatgccga ctccctgcct gtcggagcag gcggtatggt
     4921 cggcatacgc gtcggcgcag tgtttgcgtg gctgttgggg agatcgggca gcctgctgat
     4981 tatcttggtt gttctgctgt tgtcgttgtc cctgctggtg cagatttcat ggctggaatt
     5041 tttgaacggt gcgggcaggg cggttcaaaa ccgcctgagt gccttatccg gcaaggtcat
     5101 ggctttagga aaacgccggc cgaatagcaa aacagacggt gtcgataccc aaaatacacg
     5161 gcgcatggta aaagaagcca agaatattac ggccaaaccc gttgccttgc ccgaaggcag
     5221 cagcagcaac cgcaaatccg tcgcggtttc cgtcgcgccg tcgcccaaaa ttcaggtttc
     5281 tctgtttgaa gatgacgaac ctcggcaggc gggcgaatac cacaagccta cattgaacct
     5341 attgcggatt cctgacagcg aacccgtcag catcaatccc gccgaattgg agcgcactgc
     5401 cgaactgatc gaatccaaac tggcagaatt cggcatcggc gtacaagtcg tatccgccac
     5461 atccggcccc gtcatcacgc gctacgaaat cgaacccgcg caaggtgtta aaggcagcca
     5521 aattgttgcc ttgtcgaaag atttggcacg ctctatgtcg ctgcaatccg tgcgtatcgt
     5581 cgaaaccatc gcaggtaaaa acacgatggg catcgagttg cccaacgaca aacgccaaga
     5641 cgtgatgttg agcgaaatct tgtcctcgcc cgtgtttgcc ggagccaaat ccaagctgac
     5701 cgtcgcgctg ggcaaagaca ttgccggtac ccccgttgtc ggcgacttgg cgaaaatgcc
     5761 gcaccttttg gtcgccggta tgactggttc gggcaagtcc gtcggcgtga acggcatgat
     5821 tatgtctatg cttttcaaag ctacgcccga cgaagtccgc ttcattatga tagacccgaa
     5881 aatgctcgag ttgagcattt acgacggcat tccgcacctg ctctgtcccg tcgtgaccga
     5941 tatgcgcgaa gcagggcagg cgttgaactg gtgcgtcgcc gaaatggaaa aacgctaccg
     6001 cctgctttcc cacgtcggtg tacgcaatct ggaaggcttc aaccaaaaag tcgaagccgc
     6061 aaaagcggca ggcaagccgc tgctcaatcc gttcagcctg aaccccgacg agcccgagcc
     6121 gctggaaaaa ctgccgttga ttgtggtcgt tatcgacgag ttggcagacc tgatgatgac
     6181 cgaacgcaaa gccgtcgaac agcaaatcgc ccgtctcgcc caaaaagcgc gcgccgccgg
     6241 tatccatatg attgtcgcga cccaacgtcc cagcgtcgat gtcgttaccg gcctgattaa
     6301 agccaacatc ccgacgcgta tggcgtttac cgtgcaaagc aaaatcgaca gccgtaccat
     6361 cctcgaccaa atgggcgcgg acgaactgct caaatatggc gattcgctgt tcctccagcc
     6421 cggcagtgcc gaaccgaccc gcctgcaagg cgcgtttgtt tcagacgacg aagtacatca
     6481 agtcgtcaac tacgtcaaat cgcaagcccc agccgactat attgaaggtc tgctcagcgg
     6541 cgaggccgcg ctggaaactg ccaatatcgt taatccgaat gcagacagcg acgaattgtt
     6601 cgatcaggca gtcgcctatg ttttggaaag caaaaaaacc tccatttcgt ctttgcagcg
     6661 gcagttgcgt atcggctata accgcgcggc aaacctgatg gaggcactgg aaaatgcggg
     6721 tgtcgtttct cccaccgacc tcaacggcag ccgtaaaatt ttggcgcaca aggaccattt
     6781 gtagcccgta ttgcaaaatg ccgtctgaac ggcggaattg gcgtttcaga cggcatatta
     6841 tgtttcaggc gaaacattgt gatatacttg ccagctaaaa tttccccttt gcggcaatgc
     6901 ggttcaaata tcgtaccgtt gcgctgtttg cttccccatg tagggaagaa agtttatcat
     6961 tttatcaaca caacaaattt aagggcttat gatgagcgta actgttgaaa ctttagaaaa
     7021 tctggaacgc aaagtagtgt tgtccctgcc ttggtccgaa atcaacgcag aaaccgataa
     7081 aaaactgaaa caaacccaac gccgtgcaaa aatcgacggt ttccgtccgg gtaaagcacc
     7141 tttaaaaatg attgcccaaa tgtacggtgc gagcgcacaa aacgacgtga tcaacgagct
     7201 ggtgcaacgc cgcttctacg atgttgccgt tgcccaagag ttgaaagtgg caggcttccc
     7261 ccgttttgaa ggcgttgaag aacaagacga taaagagtct ttcaaagttg ccgccatttt
     7321 tgaagtgttc cccgaagtcg ttatcggcga tttgtctgca caagaagttg aaaaagtaac
     7381 cgcttccgtc ggtgatgccg aagtggacca aaccgtagaa atcctgcgca aacaacgcac
     7441 ccgcttcaac catgtcgaac gcgaagcccg aaacggcgac cgcgtcatca ttgactttga
     7501 aggcaaaatc gacggcgaac cttttgccgg cggcgcatcc aaaaactacg ccttcgtatt
     7561 gggcgcaagt caaatgctgc ctgaatttga agccggcgta gtcggcatga aggctggcga
     7621 aagtaaagac gttaccgtca atttccctga agactaccac ggtaaagacg ttgccggtaa
     7681 aactgccgtg ttcaccatta cgctgaacaa cgtttccgaa gcgactctgc ctgaagtcga
     7741 tgcagatttt gcaaaagcct tgggtattgc ggatggcgac gttgccaaaa tgcgcgaaga
     7801 agtgcagaaa aacgtaagcc gcgaagtgga acgccgcgta aacgaacaaa ccaaagaatc
     7861 cgtaatgaac gcgctgctca aagccgtaga gctgaaagca cctgttgctt tggtcaatga
     7921 agaagccgca cgcttggcaa acgaaatgaa acaaaatttt gttaaccaag gtatggctga
     7981 tgctgccaac ttggatctgc ctttggatat gttcaaagaa caagccgaac gccgcgtatc
     8041 tttaggtctg attttagcca aactggttga cgaaaacaaa ctggaaccga ctgaagagca
     8101 aatcaaagcc gttgttgcca actttgcaga aagctacgaa gatcctcaag aagtgattga
     8161 ctggtactac gcagatcctt cccgcctgca agccccgact tctttggcgg tagaaagcaa
     8221 cgtcgttgat ttcgttttgg gtaaagccaa agtaaacgaa aaagctttgt cttttgacga
     8281 agtgatgggc gcgcaagcct gattatcctg aaaatgccgt cttagggcag tttttgaaag
     8341 caccgaagcc gcttttaaag cccgctttgg tgcttttaca tcatgaaagg agacgaaatg
     8401 tcttttgata actatcttgt ccctaccgtt atcgagcaga gcggtcgcgg tgagcgtgca
     8461 ttcgatatct attctcggct tttgaaagag cgcatcgtat tcttggtcgg cccggtaacc
     8521 gacgagtccg ccaatctggt ggttgcccaa ctgttgtttt tggaaagtga gaatccggat
     8581 aaggatattt tcttctatat taactcgcca ggcggttcgg taacggccgg tatgtcgatt
     8641 tacgacacca tgaattttat caagcccgat gtatcgactt tgtgcttggg gcaggcagca
     8701 agtatgggcg cgttcttatt gtcggcaggc gaaaaaggca aacgtttcgc cctgcccaac
     8761 agccggatta tgattcacca gcctttaatc agcggcggct tgggcggtca ggcatccgat
     8821 attgaaattc acgcacgcga actgttgaaa atcaaagaaa aactcaaccg cctgatggcg
     8881 aaacattgcg gccgcgattt ggcagatttg gagcgcgaca ccgaccgtga taatttcatg
     8941 tctgccgaag aagcaaaaga atatggtttg atcgaccaag ttttggaaaa ccgcgcttct
     9001 ttgcagcttt aaaccttgtg aagtagacaa atgccgtctg aaaacagttc agacggcatt
     9061 tgtcttatag ggactgtatt gggatgattt tgttctggtt gcgtttggag ccgttttcgc
     9121 cgtagttggt tttgacatac tcttcaacaa tagccaaaaa ttccgttgca atgttgtcgc
     9181 ctttcagcgt tactttgcgt tcaccatcta cataaacagg cgcgacgggt gtttcccccg
     9241 taccgggcag gctgatgccg atgtcggcca atttgctttc tccgggccca ttgacaacgc
     9301 agcccattac ggcaacgttc agggattcaa ccccgggata aagggtacgc catatagaca
     9361 ttttttggcg caggtaattt tgaacatctt gtgccagctc ttgaaatacg gtactggtgg
     9421 tacgcccgca gccggggcag gcggtaacca tcggcgtaaa cgaacgcaat cccatagtct
     9481 gtaaaatctc ttgcccgacg acgacctcct gagtacgcgg gctgccaggt tccggagtca
     9541 gtgaaatgcg gatggtgtcg ccgattcctt cttgaagcaa gacggataat gccgccgttg
     9601 atgcgacaat gcctttgctg cccataccgg cttcggtcaa acccaaatgc agcggatagg
     9661 cgcaacggct gcccagttcg cggtaaacct gaatcaaatc ctgaaccgcg ctgactttgc
     9721 acgacaggat gattttgtct tcgggcagtc ccaatagaac ggctttttcg gcagattcca
     9781 aagcggagac aatcagtgct tccttcgtca cttcttcggg cggtttcggc gcggaagaag
     9841 cgaggttggc atccatcata cgtttggcga ggctttgatc caaagaaccc cagtttacgc
     9901 cgatgcggac ggctttatcg ttttcagcag cggtacgaat cataaaggca aatttttcat
     9961 cgccttttac gcctttgccg acattgccgg gattgatgcg gtatttggac aatgctttgc
    10021 cgcattcggg aaattccgcc aacaggcgtt cgccgttgaa gtggaaatcg ccgataagtg
    10081 gtgtggcata gcccatatcg tccaagcggc ggcggatttc ggcaactttg gacgcggctt
    10141 cggggctgtt gacggtaata cgcaccattt cggatccggc atcgctcaat tccttaatct
    10201 gcaatgcggt ggcttttgca tcggcagtgt cggtgttggt catagattgg ataacgacgg
    10261 gtgcttctga accgacggta atatgatcga tgcggacttg atgcgtcttg cggcgttgga
    10321 gtgtgttcat atggtttgaa tctgtttatt gaccgatgag gacggtttgc aattcttccg
    10381 agtaggggaa atttgcctgc aattgtgctt catattcgta tgccgcctgt gcgttgccga
    10441 gggctttggc aattttccag cctagcagca aatcatcggc ctgaaggact tctaccctgc
    10501 tttggtattt tttaaagtag taatcggcat cgcccaactg cccggccagc attttggtgc
    10561 gcgccagttc tttaaatgcg ggtgggaact gcggctgggc ggcgagggaa cgtttcaaat
    10621 aggcttccgc caatccgaat tgcccctgtt ttgcgctgca tatgccttta ttcaggttgg
    10681 caatataagg ggtcgggtag gtggggtcgg ccagggcttt gtcgaaatat gccatagatt
    10741 cggcagggcg gttgagcctg ccgcacagga accagccgta gttgttgttg atttcggcac
    10801 tgtcgggttt gatggagagg gcttgccgga aactttcctg cgccttgtcg ttaactttca
    10861 ggtattggta gatttcggca cggaccagcc aggcaagctc gtttttaggg tccgatttca
    10921 gggcgtcttc aatacttgcc gtcgcctgac ggtagtcctg accgcgcata tattccattg
    10981 ccaactgggt tttgatattg gaaacctgat tggctttttc tgcccgcgag gggcggtagg
    11041 aagtgctgca cgcgcccaag gcaaggacaa gtaataaaga gattcgtttg gatggcttaa
    11101 aaggcataat tacccctgtt gtccgattaa aatctgctgc catttttgtt ggcggcgcgt
    11161 tttatcctga acctgccccg ccaactgtcc gcaggcggca tcgatgtcgt cgccgcgcgt
    11221 ttttcgtacg gtaacgacaa atcctgcctg ctgcaaaata tcgcggaaca cacggatgtt
    11281 ctcattgctg gagcgttcgt atccggagtt tgggaaggga ttgaacggaa tcagattgaa
    11341 cttgcaggga acatctgtga ccagttcgat cagttcgcgc gcatgttgcg ccttatcgtt
    11401 tattccgtcc aacatgacgt attcgaaagt gatgaaatcc ctgggtgctt tgaccagata
    11461 gcgttggcat gcggccatca attctttcaa gggatatttt ttgttcaacg gtacgatttg
    11521 gttgcggact tcgtcattgg aagcgtggag ggaaaccgcc aaagccaccg gcatgacatc
    11581 gcgcaacctg tccatttggg gaaccatacc cgaagtggaa acggttacgc ggcggcggct
    11641 caaaccgtag ccgtggtcgt ccagcatgat gcttaaggcg gtaacgacat tgtcgaagtt
    11701 cgccatcggc tcgcccatgc ccatcatgac gacgttggaa ataacgcgct cgtttttcgg
    11761 tgtaacgccc atggctttgt ttgcccacca caattgcccg atgatttcgg cagaggtcag
    11821 gttgcggttg aagccctgcc ggccggtcga acaaaatgta cattccaaag cgcagccgac
    11881 ttgagaggaa atgcagagcg tgccgcgatc cgattcgggg atgaagacgg tttccacgcc
    11941 gttgcccgta ccgacatcca aaagccattt tcgagtgccg tctgaagatt tttgagacat
    12001 catcagcttg ggaatttcga tgcctgcctg ttcgttcagt ttatggcgca acgattttgc
    12061 caaatcggtc atttcgtcaa aattttgcgc gccggattgg tgcatccaac gcataacctg
    12121 tttggcacgg aaaggttttt cgcccatatc ggcaaaatgt cgggtcagcc cttgaaggtc
    12181 gtagttgagc agattggttt tcatgtgttt gtgtttttct taaatcaagg cttcaaataa
    12241 aaaattgcag ggcaggcaag tttgatttga agccttttgc aacagagttt tcagacggca
    12301 tggatatatt tatgccgtct gaagaggagt tgggcggtgt attgtatcaa cgggggcaga
    12361 tttcggtttg gctgaaaaag taggcaattt ccaaagcggc attttccacg ctgtcggaac
    12421 cgtgtacggc attaatgctg accgaagtgg caaagtccgc gcgtatcgtg ccttcggcgg
    12481 cttcggaagg attagttgca cccatcagtt cgcggttttt caggacggcg ttttcaccct
    12541 ctaatacctg aatcataacc ggaccgccgg tcataaattc aaccaatccg gcgtagaagg
    12601 ggcggtcttt atgaaccgca taaaattctt gcgcctcttt gagagtaagc tgcttcattt
    12661 tggcggcaac gattttcaga ccgttctcct caaagcggct gtatattttg ccgataacat
    12721 ttttgccgac ggcatcgggt ttgatgatgg agatggtacg ttcaatcgcc atgctatatc
    12781 cttattttta cttaagaaga atcaaatcgg tattctatca aaaaaataac ttatccgcct
    12841 gaagccttgg gagatagggt caggtgcggc tgttccaaat aaacttcgga ctgcatttcg
    12901 accatccggc tggcggtgcg ggtaaattct tcggcaaaat cgccttccgt atagatatgg
    12961 ttgacatcta ccgcgccggt ggcacacagt ttgacccgga aatcgtagag tacgtcaatc
    13021 agccaagtca gccgccgcgc ctccgccttt tcttgcggtg agagttgttc caaacctgaa
    13081 ataaaaacca tttcataatg ttcggccaaa tacagatagt cggactgtga gcgggggccg
    13141 aagcacagtg cgcggaaatc aaaccatatg gcacggccgg actcggcttt gtggggaatc
    13201 tcccgaccgt ggatggtgct gatgccgggg ttcaaatcgg taatgcctgt catttctttg
    13261 aacagttttg ccagttttgc ctcattttct tcattggcag gcgtaaagaa aatctcggcg
    13321 gggcggaggg tacgcagtcg gtagtcttca ccgccgtcaa cgtttaagac ggtcaggctg
    13381 gactcgatga gcgcgattgt gggaagaaaa ctgctccggt tttgaccttg cgggtagagt
    13441 tcggaaggcg cgtagtttga agtcgccacc aaaacaacgc cctcgttaag caggttttcc
    13501 agcagacggc ctaaaatcat tgcatccgca atatcgctga catgaaattc gtcaaaacat
    13561 aatacgcggg tttctttggc aatctcggcg gcaacggatt tcaacgggtt gctttcgctt
    13621 ttcagggttt tcagcctctg gtggatttct gccataaagg catgaaagtg gacgcggcgt
    13681 ttgcggcggt aagggaggca gccgaaaaaa gcgtccatca gaaagctttt gccgcgtccg
    13741 accccgccat agaaataaag ccctttgggg acttgcgggg aacgcaaact cctgcctaaa
    13801 aaacggtttc ttttgcgttt gaacatcatc aattcggtcc aaagccgatc gaggtgttcg
    13861 atggcagctg cctgcgcgtc gtcgcggatg aagttgggca gttgtgaggc agcctgatac
    13921 caagtcagcg ggctgtgatt ttcaaacggc ggggctttaa aaagttggtc tctattactc
    13981 atatttaacc ttgttatact tcttgcggct aagaaagaaa atgcttaccg tcggtattat
    14041 aaatgatttc cttcaaactt ggcattttcc gtggaaaaga ataatgacgc gcacaagggg
    14101 gtataagcaa aggcggcgtt tcaatatggt gcagatatga gctcaaatgc cttttatagt
//
LOCUS       DAXHSJ010000037        13709 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000037 DAXHSJ010000000
VERSION     DAXHSJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.037"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(69..2045)
                     /locus_tag="ACFM8U_000810"
     CDS             complement(69..2045)
                     /locus_tag="ACFM8U_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC6334078.1"
                     /translation="MNEFSLAPIVIVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEGIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGETGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="ACFM8U_000811"
     CDS             2194..3033
                     /locus_tag="ACFM8U_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC6334079.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GATVIRDISALLGKPFVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARTRYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3234..4265
                     /gene="gap"
                     /locus_tag="ACFM8U_000812"
     CDS             3234..4265
                     /gene="gap"
                     /locus_tag="ACFM8U_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6334080.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGKLDGLAIRVPTVNVSLVDLNFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4415..5140)
                     /gene="aat"
                     /locus_tag="ACFM8U_000813"
     CDS             complement(4415..5140)
                     /gene="aat"
                     /locus_tag="ACFM8U_000813"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC6334081.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYQN
                     GVFPWFLRDGWFFWYAVGPRAVIVPERLHVPCSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAMPLKEEIGRREVACRGL"
     gene            complement(5210..5644)
                     /gene="fur"
                     /locus_tag="ACFM8U_000814"
     CDS             complement(5210..5644)
                     /gene="fur"
                     /locus_tag="ACFM8U_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC6334082.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            5863..6240
                     /locus_tag="ACFM8U_000815"
     CDS             5863..6240
                     /locus_tag="ACFM8U_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6334083.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            6250..7059
                     /gene="dapB"
                     /locus_tag="ACFM8U_000816"
     CDS             6250..7059
                     /gene="dapB"
                     /locus_tag="ACFM8U_000816"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC6334084.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDTVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIQAAGEKTGVVFAANFSVGVNLTFHLLDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7308..7703)
                     /locus_tag="ACFM8U_000817"
     CDS             complement(7308..7703)
                     /locus_tag="ACFM8U_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334085.1"
                     /translation="MPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFPQEIPT
                     PNYTDWVKIKFKQFSYLKFIYGYATKSQDKDIDNILELGELKQDDEILDYGGTLELIG
                     GRCDLLTGFSIDIVCREIELEFLDQESFN"
     gene            complement(7721..7966)
                     /locus_tag="ACFM8U_000818"
     CDS             complement(7721..7966)
                     /locus_tag="ACFM8U_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334086.1"
                     /translation="MDYLLEAARITKEGGEIVINGTSNNKYLRGIPNEAELARMGLRL
                     KYNGLLKEEFKQLKFHLSNGGDLRSSNYKTIVFEKVK"
     gene            complement(7974..9146)
                     /gene="lpxB"
                     /locus_tag="ACFM8U_000819"
     CDS             complement(7974..9146)
                     /gene="lpxB"
                     /locus_tag="ACFM8U_000819"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC6334087.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFAEVVKRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAERLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMESQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRKTARKTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLKRYPAARFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9194..10186)
                     /locus_tag="ACFM8U_000820"
     CDS             complement(9194..10186)
                     /locus_tag="ACFM8U_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6334088.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFEAVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEARYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVRLPLFKYTGAQGEKMVRVSEDGQAAH
                     TVFRVLNRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPLDLAQFAVMLENGTKM"
     gene            10680..13439
                     /locus_tag="ACFM8U_000821"
     CDS             10680..13439
                     /locus_tag="ACFM8U_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC6334089.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVHAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRIDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETTTVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSENATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVLAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
BASE COUNT         3453 a         4466 c         3202 g         2588 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccggttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cattggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccaaac cggcggcttc caatacttcc
      661 cggcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacgcgc
      721 gcgatgtcca aatcgagtgc gaaatacggg ataccctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatgat cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatta atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aagatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgccagcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacgggacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccaaacac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaatcacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgcattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggcgc aaccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc tttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcac gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acgcccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat acctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgagccctcc cctgatatgc cgtctgaagc
     3061 cgcttcagac ggcattttat caagctctcc gtccgccgca cccgtgccgt ccgcatctta
     3121 ccgcccaccc ttccggccgc gccgttttca ataaaatatt aattacacgc cactacaaat
     3181 ttgctataat tcgccccgaa aatctaccca acccctcaac aaaggaacga accatgggca
     3241 tcaaagtcgc catcaacggc tacggacgca tcggccgcca ggttttgcgc gccatctacg
     3301 actatcagat tcaagaccaa ctccaaatcg tcgccgtcaa cgccagcggc agccttgaaa
     3361 ccaacgccca cctcaccaaa ttcgacaccg tgcacggacg ctttgaagcc gacgtatccc
     3421 acgacggcgg caacctcatc gtcaacggcg acaaaatccc cttcttctcg acccgcaacc
     3481 ctgccgaact gccgtggaaa gaactcggtg tcgatttggt catggaatgc accggcgcgt
     3541 tcaccagcaa agaaaaagcc aaagttcatc tggaaagcgg cgcgaaaaaa gtcctcattt
     3601 ccgcaccggg cggcgacgat gtcgatgcaa ccgtcgtgta cggcgtgaac gacagcgtcc
     3661 tgaccgccga catgaccgtc gtttccaacg cttcctgcac caccaactgc ctctcgccgg
     3721 ttgccaaagt gttgagcgaa agcgtcggca tcgtcaaagg cgcgatgacc accatccacg
     3781 cgctgaccaa cgaccaaacc gttaccgacg tgcgccacaa agacctgcgc cgcgcccgca
     3841 gcggcgtgga aaacatgatt ccgaccaaaa ccggcgcggc aaaagccgtc ggactggtac
     3901 tgcccgaatt gaaaggcaaa ctcgacggac tcgccatccg cgtgccgacc gtcaacgtat
     3961 cattggtaga tttgaacttc caagccgcgc gcgacaccac agtcgaagaa atcaacgcac
     4021 tgatgaaagc cgcctcggaa gcgggtgcgc tcaaaggcgt tttgggctac aacaccctgc
     4081 ccttggtttc catggacttc aaccacacca ccgaagccag ccacttcgac gcaacgctga
     4141 ccaaagtcgt cgacggcaac atggtcaaag tcttcgcatg gtatgacaac gaatggggct
     4201 tcagctgcca aatgctgaac accgcacgcc gtatgttcgg acttgaagtg cgcccgctca
     4261 aataagcaat aaaccgtcaa acaaaatgcc gtctgaaacc cgatatcgat gttttcaagt
     4321 ttcagacggc atttttcacc gtgattttac ccggctgtcg tcatttccaa ttttaatccg
     4381 ccatatttga acctgaccga agcataagcc gccatcaaag ccccctgcac gccacttcgc
     4441 gccgcccgat ttcctctttc aacggcatgg cgttcaacat ccgcagacgt tcggcaaaat
     4501 ccgcaaacgg cagcagctcc gaaccgaaac ggcgcatatg ttccgtgtcc tgctggcagt
     4561 ctatcagttc cacgcccaaa tccgccaaaa acggcacggc gcaggcaaac gcgattttcg
     4621 acgcatccgg ttgcaacgca aacattgatt cgccataaaa caccctgccg atttgcacgc
     4681 cgtaaaagcc gcccgccaac ctcgtttcac cgctttcatc gggataatgg cactcgaaag
     4741 aatgcgcgtg tcccatttcg tgcagcttca aatatgccgt ctgaaactcg ggcgcaatcc
     4801 aagttccgtc ctgattcggg cgcgccgctg ccgcacaatg cgcgaccact tccgcaaaac
     4861 agccgttgac tgcaacccga tagctgccgt tgcgtagcgt tttcgccagc gagcacggaa
     4921 cgtgcagacg ttcgggaaca atcaccgcac gcggcccgac cgcataccaa aaaaaccaac
     4981 cgtcccgcaa aaaccaagga aacacgccgt tctgatacgc ctcgagcagc cgccccgcgt
     5041 ccaaatccct gctcacgccg accagcccgt cgcaccgtgc caaagcatag gcaggatcgg
     5101 gaaaggcata attgtcaggg gcaagcagcg gaatacgcat ggcagttctc caaaaacaca
     5161 gacggcatcc aaaatcatga atgccgtctg aacaacaaac cgtccggatt taacgtttgc
     5221 ccttggcctg acagtcgctg cacacgccgt acatatacag cgcgtgatcg acgatgcggt
     5281 agccgttttc ctcggcgatt ttgtcttgca gggcttcgat ttcgggattg tggaactccg
     5341 ttacctcgcc gcacttcacg cagacgatat ggtcgtggtg gtcgcccttg tccaactcat
     5401 aaaccgcctt gcccgtttca aaatggtggc gttgcaaaat gcccgcctgc tcaaattgcg
     5461 tcagcacgcg gtaaatcgtc gccacaccga tttccacacc ctcttccaac aaaatgcggt
     5521 acacatcttc cgcactcaaa tgctcttccg catgcgtctc gaacaaatcc aaaatcttca
     5581 aacgcgggcc ggtaaccttc agaccgctgt ctttcagttg tgcaatattg ttgaattttt
     5641 ccataatatt caatacccct gtaaaacaat agacgttata atacgcaatt tcggcctgtt
     5701 tgcccactat cgcaccatag cagtttgcaa tagtaaaacc gcaccggcgg ccgatgcgcg
     5761 gacagggcgg ataccgctta atcgtatgat tatcgttcgc ttttataaaa tattcaagca
     5821 gtgttacact acccatcccg atttgcacag aaaggcattt ccgtgaacaa aaccctcatc
     5881 ctcgcccttt ccgccctcct cggccttgcc gcgtgcagtg ccgaacgcgt ttcactgttc
     5941 ccctcgtaca aactcaaaat catacagggc aacgaactcg aaccgcgcgc cgttgccgcc
     6001 ctccgccccg gcatgaccaa agaccaagtc ctgctcctgc tcggcagccc catactgcgc
     6061 gacgcattcc ataccgaccg ctgggactat accttcaaca cctcccgcaa cggcatcatc
     6121 aaagaacgca gcaatctgac cgtctatttt gaaaacggcg tactcgtccg caccgaaggc
     6181 gacgtcctgc aaaacgctgc cgaagccctc aaagaccgcc aaaacacaga caaaccataa
     6241 ggaacacaca tgacaccgct caaaatcgcc atcgccggcg caaacggccg tatgggacgc
     6301 gtattggttg aagccgtcaa caaccatccc gacaccgtcc tctccggcgc gctcgaacac
     6361 tcgggctcag aagccctcgg gctggacgca ggctacgccg tcggactcaa aaccggcatc
     6421 gccatttcag acgacgttga caccgttctc gcacaaagcg acgtactcat cgactttacc
     6481 cgccccgagc cgaccctcaa acacctgcaa aaatgcgttg aaaaacaagt caacatcatc
     6541 atcggcacaa caggcttcga cgatgcgggc aaagccgcca tccaagccgc aggcgaaaaa
     6601 acaggcgttg tcttcgccgc caacttcagc gtcggcgtca acctcacctt ccacctcctc
     6661 gacaccgtcg cccgcgtatt aaacgaaggc tacgacatcg aaatcattga aggccaccac
     6721 cgccacaaag tcgatgcccc cagcggcacc gcgttacgca tgggtgaagt catcgccggc
     6781 gcgctcggcc gcgacctcaa acaatgcgcc gtttacggcc gtgaaggcca caccggtccg
     6841 cgcgatccgt ccaccatcgg ctttgccacc gtccgcgcag gcgacatcgt cggcgaccac
     6901 accgccctct tcgccaccga cggcgaacgc gtggaaatca cccacaaagc cagcagccgc
     6961 atgacctttg ccgccggtgc cgtccgcgcc gcagtttggg taaacggcaa aacgggtttg
     7021 tacgatatgc aggatgtact cgggctgaac aaccgttaac ccccatacaa aataccgtct
     7081 gaaaagatat tgttcagacg gcattttgcc gacaggctcc gtatcggcat atcaatgttt
     7141 caacacacag gacgacacat aaagcgtcgc cctatgtgtt gccctgattc ggaaggggtt
     7201 acgcccctcc caaataaagt ctgattctac cgccctaaag ggcggggttt caaccgaaaa
     7261 ggaaatacga taaagttgcc tgcgcagcat aacaagttac ggctcgttta attgaaactc
     7321 tcctgatcta aaaattctaa ctctatttcc cggcaaacta tatctatact aaaaccagtt
     7381 agaagatcac atctaccgcc tatcaattcc agcgtacctc cataatccaa gatttcatca
     7441 tcctgcttta attcgccaag ctccaatata ttatcgatat ctttatcttg gctctttgta
     7501 gcgtatccat agataaattt cagatagctg aactgcttga atttaatttt tacccagtct
     7561 gtataatttg gtgtaggaat ctcttgagga aagtccggat aatccacatt ctggacgatg
     7621 atatggaatt catcgttttt caaagtatag tttaatagag tgccctcata tatatacaga
     7681 aattgataat ctttttttgg aatagtatta agttgtcata ttattttacc ttttcaaaaa
     7741 caatggtttt ataattactt gatctcaagt cgcctccatt gctcagatga aatttcagtt
     7801 gtttaaactc ttcctttaac agaccattat attttaacct taatcccatt cgggccagtt
     7861 ctgcttcatt tggaattcct cttaaatact tattgtttga tgtgccatta atcacaattt
     7921 ctcccccttc ttttgtaata cgtgctgctt caagaagata atccattaac cgctcaacat
     7981 cccgcctctt ccaaaaccgc gcgcgcggcc aaatccgccg tatctttttt caacagcagg
     8041 tgcaacgccc tgaaatcctg ttgcagcgcg gcaaccttat cggggtgttc gtaccagtcc
     8101 gccaacgccg ccgccagttt ttccggtacg gcatcatgtt gaagcagctc cggcacagcc
     8161 tccttaccca acaggatatt cggcaggccg acatgcggca ctttgatttt gcgtttcaca
     8221 taagcatagg tcagcggcga aatcttgtag ctgatgacca tcggacgctt gcacaacgcc
     8281 acctccaaag tcgccgtacc gctcgttacc agcaccgcat ccgccgccct gcacaccgtt
     8341 tcagactgtc tgtcgattac cgtcagcggc aatccggcaa actccggacg ctgcaaaact
     8401 tccgccaaac gccgcttcgt cgcctcagtt gcggcaggca gcaggaagcg tgcggcagga
     8461 tagcgtttca acaacaataa tgccgtctga aaaaacaccg gcgccatata atcgatttcg
     8521 ctgacgcggc tgccgggcag cagggcgaat acggggatgc cggcatccac gcccaaagtt
     8581 ttccgcgccg ttttacggtc gtcttccaag ggcataagct gcgccatcgg atgaccgaca
     8641 aactccgcac gtccgcccgc atcgagataa agctgcgact ccatcgggaa caggcacagc
     8701 acgcggttga cctgatgcac gattttgccc acgcgttccc gccgccacgc ccacaccgac
     8761 ggactgacat aatgcagggt cggaatgccc gcccgtttga gccgctccgc cacacccaga
     8821 ttaaaatcgg gcgcatcgat gccgacaaac acatcaggtt tcaacgacag caaatcccgt
     8881 accagccccc tgcgtatccg taaaatttcc ggcaggcgtt ttaccacctc ggcaaagccg
     8941 cgcaccgcca gtcgctcctg atcataaagg ctctcgaaac cttccgcctt catcagttcg
     9001 ccgccgatac cgacaaaacg cgcctgcggg caacgctttc ggatggcgcg tatcaggtgc
     9061 gcccccaaca ggtcgcccga cgcttcgccg acactgacag caatcaaagg gcttttttta
     9121 tcaaccatat tcgtctgtcc ccacatactt tcacgtcccg atgccgtctg aaggcttcag
     9181 acggcatcgg ggttcacatt ttcgtcccgt tttccaacat caccgcaaac tgtgccaagt
     9241 ccagcggcag ctccgccttc aacaccagcg gctcgcccgt gagcggatgg ttcaggtgca
     9301 gctcggacgc gtgcaaaaac atccgcttca aacccaactt ctgcaaacga cggttagcct
     9361 gataatcgcc gtagcgttcg tcgcccgcaa tcggacagcc ttgagattgc agatggacgc
     9421 ggatttggtg cgtacgaccc gttttcagcg ttgcccgcac caaagtcagg tgcgacagcc
     9481 cgacaccgtg caaaatgccg tctgaaaacc gatttaacac acggaacacc gtatgcgcag
     9541 cctgcccgtc ctcgctaacg cgtaccatct tttcgccttg tgcgcccgtg tatttgaaca
     9601 gcggcagcct gacgtggaaa ttatcatccg gcagcttgcc cacgcccaat gcgagataga
     9661 tttttttggg gtggtcgttg cggatggctt cgtgcagttt gacgagcgcg ctgcgtttct
     9721 tcgccaccat cagcaagccg ctcgtatcct tgtccaaacg gtggacgagt tcaagataac
     9781 gcgcctccgg acgggcgcgg cgcaactgtt cgataacgcc aaaactcacg ccgctgccgc
     9841 catgcaccgc cacgccggaa ggcttatcga tcaccaaaag cgcgtcgtcc tcatacaccg
     9901 cctcaaacgc acgcgccggt acggcggcac gcctttcaga cggcatttcc ttctccgcca
     9961 cgcgcacagg cggaatccgc accgtatccc cctccgcaat acggctgtcg ggtttgcagc
    10021 gtttcttgtt caaccgcacc tcgccggcgc ggataatgcg gtggatatgg ctcttgggta
    10081 cgcccttgag gatttttatc agatagttat caaggcgttg ccccgcctca tgttcggcaa
    10141 cccctatcaa gctgaccgaa tctttgctta tttcgtgcgt tttcatctat aatccgaaca
    10201 tccgtttcag caaaaagcgc gcccgccgcg cccccctgaa acggtttact ttaaaacgga
    10261 attttatatt aaaaacgcac tccgcaaagc attttccctg cgcctgttcc cagccggcag
    10321 gcgcagaacg taatcaagtt tgaattgatt ttgccgtttc ggcgcggaag taagacggca
    10381 gccgggcccc aagtcccaaa cgcaaaccgc ccaaaacaca ggcatcacga taacaagaag
    10441 aagccggccc ccatttttcc gaccgcagcg ttcggaacgc ggcagacaaa ccgccgcatc
    10501 gatctttata gccttctgta tccgaccctt ccgcacacgg ttctccggaa cgtgcatccc
    10561 tgcggatttt caactcaaaa actgattacc ggttttatcc gaaaacatcg gaaaacccag
    10621 ccttacggaa tgccgaaccg gaccggcgcg cccgaatgcc gcccgtccac gaggtgatca
    10681 tgaaaagaat gttattcaac gcaacgcagg ccgaagagct gcgcgttgcc atcgtcgacg
    10741 gacaaaacct gctggatttg gacatcgaaa cgctgggcaa agaacagcgc aaaggcaata
    10801 tctacaaagg catcattacc cgcatcgagc cgtcgctgga agcgtgtttc gtggattacg
    10861 gaaccgaccg ccacggcttc ttgccgttta aagaagtctc ccgctcatac ttccaagact
    10921 acgaaggcgg acgcgcgcgc atccaagacg tgctcaaaga aggcatggaa gtcatcgtcc
    10981 aagtcgaaaa agacgagcgc ggcaacaaag gcgccgcgct gaccaccttc atcagcctcg
    11041 ccggacgcta tctggtattg atgccgaaca acccgcgcgg cggcggcgta tcccgccgta
    11101 tcgaaggcga agaacgtcaa gaactcaaag ccgctatggc gcaactcgac attccgaacg
    11161 gcatgagcat catcgcccgt accgccggca tcggccgcag cgcggaagag ttggaatggg
    11221 atttgaacta cctcaaacaa ctctggcagg cgattgaaga agcaggaaaa gcgcatcacg
    11281 acccttacct gctctttatg gaaagctcgc tgctcatccg cgccatccgc gactatttcc
    11341 gccccgacat cggcgagatt ttggtggaca atcaagaagt ttacgaccaa gttgccgagt
    11401 tcatgagcta cgtcatgccg ggcaatatag gccgtctgaa actctacgaa gaccacacgc
    11461 cgctgttttc ccgcttccaa atcgaacatc aaatcgaaag cgcgttttcg cgcagcgtca
    11521 gcctgccttc cggcggcgcg attgtgatcg accacaccga agccctcgtc tccatcgacg
    11581 tgaactccgc acgcgccaca cgcggcgcgg acatcgaaga caccgcgttc aaaaccaata
    11641 tggaagccgc cgaagaagtc gcccgacaaa tgcgcctgcg cgacttgggc ggtttggtcg
    11701 tcatcgactt catcgacatg gaaaacccca aacaccagcg cgatgtggaa aacgtcctgc
    11761 gcgacgcgct caaaaaagac cgcgcccgcg tacagatggg caaactctcc cgtttcggac
    11821 ttttagaatt gagccgccaa cgtttgaaac cggctttggg cgaaagcagc catgttgcgt
    11881 gtccgcgctg tgccggcacc ggcgtgattc ggggcatcga atccaccgcc ctgcacgttt
    11941 tgcgcatcat tcaagaagaa gcaatgaaag acaacaccgg cgaagtacac gcacaagtgc
    12001 ccgtcgatgt cgccaccttc ctgctgaacg aaaaacgcgc cgagctgttt gcgatggaag
    12061 agcgtttgga tgtcaacgtc gtcctgattc cgaacatcca cctcgaaaat ccgcactacg
    12121 aaatcaaccg catccgcatc gacgacgtag aagaagacgg cgaaccgagc tacaaacgcg
    12181 tcgccgagcc ggaagaagac gaatccgcca aacctttcgg cggcgaaaaa gccaaagccg
    12241 cccgtcccga acccgccgtc aaaggcgtgc gccacaccag ccccgccccg actgccgccc
    12301 ccgagaaaaa aacctcctgg tgggacagct tcaaagcatg gctcaaacgc attttcggcg
    12361 gcagcgaaac ccaagccgcg cccgctgccg aaacctccga aaaacgcagc acggcaaacc
    12421 gcagcggcag ccgcgccaac aaccgccgcc aaaacccgcg ccgcagcaaa cgcgaaggca
    12481 gcaaagtaga agtccgcgaa gtggcaggca aaactgccgg acaggaagcg cgcgccgaca
    12541 aagccgaaac gcgcaacaac ggcaaccgcc gccgcaacga acgcggcgac cgtgccgccg
    12601 gacgcgccga cgaagcggaa atccaagacc gcaacataca gcctgccgca accgttgcag
    12661 atgccgcacc gtccgaaacc gaagtgcaaa ccggaaaacg ccgccgcaac ggcagccgca
    12721 gcgaacgcgg ccaaaccgcg ccggaaacca ccaccgttgc cgaaacaacc gttcagacag
    12781 cggaaaacac gccgtccgaa ccgcataccg cagaagacaa aggcagcaag cccaaatccg
    12841 aacgcaaccg ccgcgaacgc gacagccgag atgccaaaga acgccgcgag cgcaacaatc
    12901 agcgcgaccg ccgtcaaaac ggcaaaaaac gcaatattcc gtctgccgcc aaaatcgagc
    12961 agtacctgaa tattcacgac accgccgaca aagtccgttc cgccgccgcg cacgttttcg
    13021 gcgaaaccga cgcaaacgcg ccgattaccg tcagcattgc cgatccggtt gcagaaaggg
    13081 atcttccgac agcatctccc gccgtttcaa acggcgacgc accggtttat gatgcggcgg
    13141 aaaaaatccg ccgtgccacc gccgccatcc tgcccgaagg cgcgacaccg aaagccgaag
    13201 cacaggaaat gccgtctgaa aacgcaacct ttacggctgc ggcggaacag gcacgggaaa
    13261 ccgcacaaac cggcggactc gtcctgatcg aaaccgaccc tgccgcattg aaggcatggg
    13321 cggcacaacc cgaagtccta gccggacgcg gtttgcgccg ttccgaacag cccaagccgt
    13381 ctgaagccgc aaccgtccct gccgaagaaa tgatccaagt cgaaacccgg caaggctgaa
    13441 ccgacggcgg caaaaagagg ttctgttccg cagaacctct tttttacatg ggttcggata
    13501 cctgcaatgc cgtctgaaac ttcgccattc ccgtgattac cgaaacattc cgccattccc
    13561 atgattcccg caacattccg tcattcccat gattcccgca acattccgtc attcccatga
    13621 ttcccgcaac attccgtcat tcccatgaaa acgggaatct agaacctcaa actttcggat
    13681 aatctttgaa tattgccgtc gcccaaagg
//
LOCUS       DAXHSJ010000038        13669 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000038 DAXHSJ010000000
VERSION     DAXHSJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13669
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.038"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..215
                     /locus_tag="ACFM8U_000822"
     CDS             <1..215
                     /locus_tag="ACFM8U_000822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334090.1"
                     /translation="LHIENAAQANFQFASLVLALSHGERGLGCRLPLRFRQILTSLIP
                     RFSFAEAALSDDLLEHHRYTHLRNAV"
     gene            complement(226..549)
                     /locus_tag="ACFM8U_000823"
     CDS             complement(226..549)
                     /locus_tag="ACFM8U_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6334091.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(628..1284)
                     /locus_tag="ACFM8U_000824"
     CDS             complement(628..1284)
                     /locus_tag="ACFM8U_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC6334092.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPDVL
                     KQQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            complement(1386..1913)
                     /locus_tag="ACFM8U_000825"
     CDS             complement(1386..1913)
                     /locus_tag="ACFM8U_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC6334093.1"
                     /translation="MYATIGDVMHALHFSASDKAALYREVLPQIESVVADETDWVANL
                     ANTAAVLKEAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGT
                     VVVGDVDAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGEL
                     AKILEKRFEASRQAA"
     gene            2073..2846
                     /gene="tpiA"
                     /locus_tag="ACFM8U_000826"
     CDS             2073..2846
                     /gene="tpiA"
                     /locus_tag="ACFM8U_000826"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC6334094.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2853..3203
                     /gene="secG"
                     /locus_tag="ACFM8U_000827"
     CDS             2853..3203
                     /gene="secG"
                     /locus_tag="ACFM8U_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC6334095.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3217..3302
                     /locus_tag="ACFM8U_000828"
     tRNA            3217..3302
                     /locus_tag="ACFM8U_000828"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3251..3253,aa:Leu,seq:gag)
     gene            4104..4472
                     /locus_tag="ACFM8U_000829"
     CDS             4104..4472
                     /locus_tag="ACFM8U_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFC6334096.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIEL"
     gene            4469..4780
                     /locus_tag="ACFM8U_000830"
     CDS             4469..4780
                     /locus_tag="ACFM8U_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6334097.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(4749..5003)
                     /locus_tag="ACFM8U_000831"
     CDS             complement(4749..5003)
                     /locus_tag="ACFM8U_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334098.1"
                     /translation="MPRGRGCFGFRARLRRALLSGYAVCTAAVSAWFDAVGHLCGYAL
                     IFGSAVLPVLAGIFSSASIVNLLILPFAKSATQWLRYPCF"
     gene            complement(5161..5895)
                     /locus_tag="ACFM8U_000832"
     CDS             complement(5161..5895)
                     /locus_tag="ACFM8U_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFC6334099.1"
                     /translation="MNAAAAKDYMKTVELNKSAGNVDTTSRTARRVQAVFRRMLPYAD
                     AANNTGHRFDWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDDEIAAIMGHEMTH
                     ALHEHGKNKVGQQILTNTAAQIGTQTILDKKPDTNPELVGLGMDILGTYGLTLPYSRS
                     LEEEADEGGMMLMAQAGYHPAAAVRVWEKMNQENDQNGFIYAITSTHPTNNARIENLK
                     RLLPTVMPVYEHSVRNKGRVNKNRRR"
     gene            complement(6112..6243)
                     /locus_tag="ACFM8U_000833"
     CDS             complement(6112..6243)
                     /locus_tag="ACFM8U_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HFC6334100.1"
                     /translation="MRDFDKPAEEAAEHAAEGAVKFIDGKPAKKIIAVPGRLANIVV"
     gene            complement(6236..6406)
                     /locus_tag="ACFM8U_000834"
                     /pseudo
     CDS             complement(6236..6406)
                     /locus_tag="ACFM8U_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(6373..7164)
                     /locus_tag="ACFM8U_000835"
     CDS             complement(6373..7164)
                     /locus_tag="ACFM8U_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC6334101.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMKR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNTIEFIGRGLYKKIDKLRPTLKAF"
     gene            complement(7393..10023)
                     /gene="leuS"
                     /locus_tag="ACFM8U_000836"
     CDS             complement(7393..10023)
                     /gene="leuS"
                     /locus_tag="ACFM8U_000836"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC6334102.1"
                     /translation="MQEHYRPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKRLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYARFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYNLLKKQVIAVGDNAFDANQWQEWYGDKENGV
                     LVNSGDLDGMNFQTAFNAIAAKLQSQDAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVGEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPAVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            10290..10805
                     /locus_tag="ACFM8U_000837"
     CDS             10290..10805
                     /locus_tag="ACFM8U_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6334103.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNNRGTFPTYDELDLMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            10812..11255
                     /locus_tag="ACFM8U_000838"
     CDS             10812..11255
                     /locus_tag="ACFM8U_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC6334104.1"
                     /translation="MPPRLLLGILCCLPIFAPAHAQGQPDTVGDFIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11424..13481
                     /gene="ppk1"
                     /locus_tag="ACFM8U_000839"
     CDS             11424..13481
                     /gene="ppk1"
                     /locus_tag="ACFM8U_000839"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC6334105.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKREHKRCPQRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
BASE COUNT         3353 a         3883 c         3373 g         3060 t
ORIGIN      
        1 gcctgcacat tgaaaatgcc gcccaagcaa actttcagtt tgccagcctc gtcctagccc
       61 tctcccacgg ggagagggga ttggggtgca ggctgccttt aaggttcagg caaattttaa
      121 cttcgttgat accgcgcttt agcttcgcag aagctgcact ttcagacgac cttttggaac
      181 accacaggta cacgcattta aggaatgccg tctgaaatgc ctgcctcaat aacgcatcat
      241 gttgccgtca atctcggccg cccatgcatc gatgccgccc tgaaggttgt acaggttttc
      301 aaaacccgcg tccgccaaat acatcgccgt atgcaggctg cggataccgt ggtggcaata
      361 caccacaagc ggcacatcat ccggcagctc gttctgccgc agcggaatca gattcatcgg
      421 gatatgcagc gcatttggca gcgaacaaac cgccgtttct tcatccgtac gcacgtccaa
      481 caaacaaaac atccgccctt cgtccatcca cgccttcaat tccgccgacc caagttgcac
      541 aatatccatc gcaccaccaa aaaaaaccaa gcaaaatgcc gtctgaagcc ccaaatccgc
      601 tttcagacgg catgacctgt caacatctta aaaatcgaaa ccgccaaacg gatcggcatc
      661 cttatcatcc aaatgcgcca ccaaggtatc gaacagcacc ttctcttcaa acacatcgcc
      721 cttgcgcgta atcaaaagcg cgcgctgaac aggcttgcgg cctacgataa ccgccatgcg
      781 tccgccatct tttaactgtt gtttcaacac atcaggcaca aggtttaccg caccgccgac
      841 ataaaccgca tcaaacggcg cacctgcgga aagttcggtc aacccgttgt tttgcacata
      901 atcgatattg tccaaaccca agccgtccaa caccgctttg gcgcggtttt gctgttcggc
      961 atcgatgtcg tccgacacca cacgaccagc caattttgcc aaaagcgcgg tcgcatagcc
     1021 cgaacccgtg ccgatttcca aaaccgtatc gtttttcgtc agcttcaagc cctgcgccag
     1081 ccgcgccacg actttcggct ccagcatctt atgaccgttg gcaagcggca gcgccatatc
     1141 cgcatacgcc aaaccctgca aatcctcatc gacaaaaagc tcgcgcggaa tctccgccaa
     1201 agcgtccaaa acgtcaaaat ccaatacatc ccacggacgg atttgctgtt cgaccatatt
     1261 gaaccgcgct ttttcaaaat ccatttagtg ctccgtcaaa taattgtttc aacaatggca
     1321 ggcattataa aaccacactc ccgccgcgcc aacttcggca cgcgccgccc gcccctttcc
     1381 atcactcaag ccgcctggcg cgaagcctca aaccgcttct ccaaaatctt cgccagttcg
     1441 cccaaataca aagcatctgt ctcatcaaac tgcgccaaat gttcgctgtc cgcgtccaac
     1501 acgccgatac agcggccgtc tgaaaacagc ggcacgacaa tctccgaacg cgacaaagac
     1561 gaacaggcaa tatggtcggg atgcgcgtcc acatccccga cgaccaccgt cccgcccttc
     1621 gcccaagcct gaccgcacac cccgcgaccg aacggaatcc gcgtacacgc caaaggcccc
     1681 tgaaacggtg ccaaaaccaa ttcgtccgaa cgcgtatcga ccaaataaaa acccacccaa
     1741 aaccaaccga acgcctcctt caaaaccgcc gccgtgttcg ccaaattcgc cacccaatcc
     1801 gtctcgtccg ccaccacaga ctcaatctgc ggcaacacct cccgataaag cgcggccttg
     1861 tccgaagccg aaaaatgaag cgcgtgcatc acatctccta tagttgcata catatcaggc
     1921 ggccattata aaacaacctg cccgaaacaa cattccaaac cgcccccgca gccgcccgcc
     1981 ccaagttgcg aacccgccgc atatccacta aacttcacat tgcaacgcgc cacacgcggc
     2041 agacagaaaa aacacgacac ggagcaaaaa agatgtatca ccaaatcgga atgtgggatc
     2101 aaaaatgggt catcggcaac tggaaaatga acggccggct ccagaacaac aattcgctca
     2161 tgcaccgctt ccgcatcctc cccaccgccg aacgcgtcct catcggactc gccgccccga
     2221 ccgtttacct gctgcaactg cacaacgcca tgcaaatcgt cttaaacaac cgcatcctca
     2281 cctgcgccca agacgtgagc cgcttccccg acaacggcgc gtacaccggc gaagtgtccg
     2341 ccgaaatgct cgccgacacc ggcacagaca tcgtcctcat cggacactcc gaacgcagcc
     2401 tttatttcgg cgaaaaaaac gaaatccaac gccgcaaaat ggaaaacgtc ctcaacgtcg
     2461 gactcatccc gttattgtgc gtcggcgaaa gcctcgaaga gcgcgaagcc ggcaaagaac
     2521 acgaagtcat cgcccatcag ctttccatcc tgcaagggct ggataccaaa aacatcgccg
     2581 tcgcctacga acccgtctgg gcgatcggca ccggcaaagt cgccaccgtc gaacagattg
     2641 ccgatatgca cgcattcatc tacaaagaaa tcttgtcttt gtgcggaagc gatgttaaaa
     2701 tccgcgtcct ttacggcgga agtgtgaaag cggacaacgc ggccgacatc ttcgcagtac
     2761 cttatgtgga cggcgcactc gtcggcggcg cgtcattgtc gtacgactcc tttaccgcca
     2821 tcatcagtgc cgcacaaaat gcgtagaaaa atatggaagc cttcaaaacc ctaatttgga
     2881 ttgttaatat aatttccgct ttggccgtca tcgtgttagt attgctccaa cacggcaaag
     2941 gcgcggatgc cggcgcgact ttcggatcgg gaagcggcag cgcgcaaggc gtattcggct
     3001 ctgccggcaa cgctaacttc ctcagccgct cgaccgccgt tgcagcaaca tttttctttg
     3061 caacctgcat ggctatggtg tatattcaca cccacacgac aaaacacggt ttggacttca
     3121 gcaacgtaca acaaactcag caagcaccca aacccgtaag caataccgaa ccttctgccc
     3181 ctgttcctca gcagcagaaa taacagtttt tcaaatgccg acatggtgaa attggtagac
     3241 acgctatctt gagggggtag tggccgtagg ctgtgcgagt tcaaatctcg ctgtcggcac
     3301 caacacacaa aacgcttaga aattctaagc gttttttttc gtttaatcct gtctaatgcc
     3361 gtcaaccaat cccaatcaat acaaggattt caggctgatt gttatcctgc cgtccccctt
     3421 cctgacagtg caatcccgtc caatccgccc taattgaact aacctgattt tttacagcat
     3481 ccgaaaatac cgcgagaaaa taccgtaaaa ataaagctga acgaccacca aatcaggaat
     3541 gccaagcggg aaatagcttg cggggaatac tgacgcaccg tagggaatga gggggaaacc
     3601 gtccaagatg cagagcggtt ttttttgggt ttttggaaaa aacctatact atagtggatt
     3661 aaatttaaac cggtacggcg ttgcctcgcc ttgccgtacc ggtttaaatt taattcacta
     3721 taaaataaga aaaagataaa aaattggtaa caaatgcggt aacaaatggt aacgaatcgg
     3781 taacaacttt tggggttttc cggtttttca ccgtcttggc agtgggagcg tagcggaatg
     3841 aaaagccaaa acgcacggaa ccgcgcctat tttgagcagg aatggcggtt aaaccgcttg
     3901 gttatatacg gggcatagga agacagcgaa acgcgctaaa aacgcaattt gagccattaa
     3961 aagcgattga ttaaaaaata atcaggttag ccgccggtgt ttcaggcggc ataaacggag
     4021 aaattgcggg gcataaaaaa ggcagcttgc cgtgttgtct gtctctggta taattccaag
     4081 tatcactaat caacggctac acaatgcgga tattcaaaaa ccaatggata gtgaaatttg
     4141 ccaagaagca caaaatcaac gattccgagc tgctggaagc ggtagagcgg gcggataacg
     4201 ggctgataga cgcagattta ggcggcggtg tgattaagca gcgcatagca aggcaaggac
     4261 aaggcagaag cggcggctat cgcagcctga tattgttcaa acaggcagat agggcatttt
     4321 tcgtttacgc ctttgccaag aacgacaggg aaaacatttc ggataaagaa ctggacgttt
     4381 accgaaaagc cgccgcatat tatctgaaat acacgcgggc agagctggcg gctttgaaag
     4441 aagacggcat tatcacggag atagaattat gaaatacaaa agcgaggcat tagccgccat
     4501 tcatgaaatg atggaagggg cttacaacat cggcgcaatc gacaaaaaga ctatgcgcgg
     4561 ctttgacaag tcctgcctga ccgaaatcaa accgttgagc ggcggggaca tcaaggcaat
     4621 cagggagaag gaggcactat cgcaagccgc tttcgccatc tatctcaacg tgggaaaaaa
     4681 tcacgtttcg gcttgggagc ggggcgttaa aaagccgagc ggcgcggcgt tgaagctgct
     4741 gaccatcgtc aaaaacaagg gtatcgaagc cattgcgtag ccgacttggc aaacggcaaa
     4801 atcagcaagt tcacaataga cgcgctgctg aatatgcctg ccaagacagg caagaccgcc
     4861 gaaccgaata tcagggcata gccgcacaaa tgcccgaccg catcaaacca agccgaaacg
     4921 gcggcggtgc agacggcata gcccgacagc aaggcacggc gcagacgggc gcgaaacccg
     4981 aaacatcccc gaccgcgagg cacggggatt ttttgcgccc gttgcagggg gattagattt
     5041 aagcggcgcg ggcttgaagg caaaacgggc ggggcgcaga actgtttaaa tgccgtctga
     5101 atctcaaacg atttcagacg gcattttgaa acaacggctc aaattctcga ctcccttccc
     5161 ttaacgccga cggtttttat taacgcgccc cttatttctg acactgtgct cataaaccgg
     5221 cataacggtc ggcaacaacc gttttagatt ttctatacgg gcattgtttg tcggatgagt
     5281 agaggtaata gcataaataa agccgttttg gtcgttttcc tgattcattt tttcccaaac
     5341 cctgacagcg gccgccggat gatagcctgc ctgcgccatc aacatcattc ccccctcatc
     5401 ggcttcttct tccaagctgc ggctataagg caaggtaaga ccgtacgtcc ccaaaatatc
     5461 catacccaat ccgaccaatt ccggattagt gtccggtttt ttgtctaata tagtctgcgt
     5521 gcctatctgc gccgccgtat tggtcaaaat ttgttgcccg accttatttt taccgtgttc
     5581 atgcagggcg tgcgtcattt catgccccat aatggcggca atttcgtcat cggtcagctt
     5641 gagtttgtcg actatccccg tataaaacgc catttttcca ccgggcatcg cccacgcgtt
     5701 cagctcatcg tttttgaaaa ccgtcatttt ccagtcaaac ctatggccgg tattatttgc
     5761 cgcatcggca taaggcagca tacgtcgaaa tactgcctgc accctgcggg ctgttctgga
     5821 tgtggtatcg acattgccgg cagacttgtt caactcaacc gttttcatat aatctttggc
     5881 agccgcagcg ttcattgtgg cagaatcatg accgtaaaca tcagcaacga ccgcacaacc
     5941 tcccagtatc gaaatgactg ccgataggca aactatctgt ctaaaggaag gaaggaagga
     6001 aatttcatat ttaggtttac tccttaaaaa attaaatttc aaaaaatgcc gtctgaatcc
     6061 aaaacggatt tcggacggca tcttaacatt gtttaatgtt tttaaaaaga tttacaccac
     6121 gatgttcgcc agtctgcccg gtacggcgat gattttcttg gcaggcttgc cgtctatgaa
     6181 tttcaccgcg ccttcagcgg cgtgttcggc agcttcttca gccggtttat cgaaatcacg
     6241 cataaattgc caataattct ccgactttct tacgactact acctttggcg gcaatattgc
     6301 gctgtacttc aactgttttc agaatggcag aagaataaat atcccttgtg aattcagtat
     6361 catgatttga aatcaaaacg ccttgagagt cggacgcaat ttgtcgattt ttttgtaaag
     6421 tccgcgacca atgaattcga tcgtattttg gtcgcgcaaa atttgcaact gttggcggat
     6481 tttgtctttg atatggttgt tttggggaaa ttggatggat agtttgtttt caaattcata
     6541 catttgcgac aatgtgaatt cttcggggag ttggtcgata catttcataa cagccaaaag
     6601 ccagcctttg cgctccgcat tttggttgcg taaaaacagg ttggattgcc attttttcag
     6661 aacggtttcg ggttcgataa tgcgggaatc gtctattaag aatatttttc cgctttcagg
     6721 caaaggggcg agattgatgg aacacataat gtggtgcggc cggtttttaa tgcctttatt
     6781 tctaggaata atcatatccg gcgtgatgaa atgtttgggt acaagcacca attgccgtat
     6841 ggagtaatcc gcttttttat atgcaagaaa gaaaaagttg gggttggtat ctgaccggat
     6901 gcgctccaac atagtgcgat atgcaccgtc aggcacgctg ttgcctatgg ttttttgatt
     6961 tttactcttt aattcatatt gctcgtggca atttgggcaa aagaggtctg caacaggttt
     7021 gttattggca aatctcttca tcggcttgct tccgcaacag gggcagtagc cgtttttttc
     7081 cagccaagcc tcgctcatta cacggatttt gtgggttgct ttattttgtt gctttcccaa
     7141 ttcagtatcg aaaaataaat tcatgttttg gattttgaga ttaagtttta gtggattaac
     7201 aaaaatcagg acaaggagac gaagccgcag acagcagggc aaggcggggc aacgccgtac
     7261 cggtttaaag ttaatccact atatttgagg tctgtcatgc ggacaaccca atctgcctta
     7321 aaaaggccgt ctgaaaccct gtttccaagt ttcagacggc ctttatccgt atggctaaac
     7381 cttaaaagcg gcttagacga cgatattcac caagcgtccg ggaacgacga tgattttctt
     7441 cgcgggtttg ccttccataa atttcaccgc gccttcggtg gcgagtgcgg cggcttcgag
     7501 gtcggctttg gatgcgtcgg cggcgacggt gattttgccg cgcagtttgc cgttgacttg
     7561 aaccatgact tcgatttcgg atttgaccaa ggcggcttcg tcgactgccg gccagcctgc
     7621 ttcccacagt ttcgcgccgt tcaattcgct ccacagggtt tcgcagatgt gcggcacgat
     7681 gggccacagc aggcgtacgg cggtttccaa tacttcttgg gcgacggcgc ggccttgttc
     7741 gccgccggtg tcggttttgt cgtattggtt gagcaattcc atcacggcgg cgatggcggt
     7801 gttgaactgc tggcggcggc cgtagtcgtc gctgactttg gcgatggtag aatgcagttt
     7861 gtggcgcagg tctttgagtt ctttagacaa accgtcttgg ctgcctgcga acgctttgac
     7921 tgcttcgcct tgcttcaagt attcgtaaac ggtacgccac aggcggcgca ggaagcggtg
     7981 tgcgccttcg acgccgctgt cgctccattc gagggactgt tcgggcggtg cggcgaacat
     8041 catgaacagg cgggcagtgt ccgcgccgta ggcgttaatc agctcttgcg gatcgacgcc
     8101 gttgtttttg gacttggaca ttttttccgt gccgctgatg acgacgggca gaccgtcggc
     8161 tttgaggacg gcggaaacag ggcggccttt gtcgtcgaac gtcagctcga catcggcggg
     8221 gttgatccaa tctttgccgc ctttgtcgtt ttcgcggtag taggtttcgc aaacgaccat
     8281 gccttgcgtc agcaggcgtt caaacggttc gccaacattg accaagcctt cgtcgcgcat
     8341 cagtttggtg aagaaacgcg cgtacaggag gtgcaaaatg gcgtgttcga taccgccgat
     8401 gtattggtcg accgcgcccc agtatttcgc ggcttctgcc gataccatgc cgtctgaaaa
     8461 cttgggcgac atatagcgga agaagtacca gcttgattcc atgaaggtgt ccatggtgtc
     8521 ggtttcgcgt ttcgccgcgc caccgcagca tgggcaggtg gtttcgtaaa actcgggcat
     8581 ttttgccaaa ggcgaaccca tgccgtcggg tacgacgttt tcaggcagga cgacgggcag
     8641 ttggtcggct gggacgggta cgtcgccgca tttttcacaa tggacgatgg gaatcgggca
     8701 gccccagtag cgttgacggg aaatacccca gtcgcgcagg cggtattggg ttttcggttc
     8761 gcccgcgtct tggctttgca gcttggcggc gatagcgttg aaggccgtct gaaaattcat
     8821 gccgtccaag tcgccgctgt tgaccaatac gccgttttct ttgtcgccgt accattcttg
     8881 ccattggttt gcgtcgaatg cgttgtcacc gacggcaatg acttgttttt tcagaagatt
     8941 gtatttggtg gcgaactcga aatcgcgttc gtcgtgcgcc ggaacagcca tcaccgcgcc
     9001 atcgccgtag ccccacaata catagttggc aatccacact tccagcttgt cgccgttgag
     9061 cggattgacg acgtagcggc cggtcggcac gccttttttc tccatcgtcg ccatatcggc
     9121 ttcggcaacc gagccggctt tgcactcggc gataaatgcc tgcaattcgg gtttgtcggc
     9181 ggcggctgcg gttgccagcg gatgctcggc ggcaacggca acataagtcg cgcccatcag
     9241 cgtgtcgggg cgggtggtat aaacttgcag gaatctcgcg taatcgcctt ccaaaccttg
     9301 tttgctgtcg tctgaaacgg caaagcgcac ggtcataccg cgagatttgc cgatccagtt
     9361 gcgctgcatg gttttgactt gttccggcca gtgttccagc ttgtccaaat cgttgagcaa
     9421 ctcttcggcg taatccgtga ttttgaagta atacatcggg atttcgcgtt tttcaatcaa
     9481 cgcacccgaa cgccagccgc gcccgtcgat gacttgctcg ttggccaata cggtttggtc
     9541 gactgggtcc cagtttaccg tgccgttttt gcgatacacg atgccttttt caaacagctt
     9601 ggtaaacagc cattgttccc agcggtagta ttcgggtttg caggtagcga cttcgcgctc
     9661 ccagtcaatc gcaaaaccca ggcttttgag ctgggttttc atgtattcga tgttgtcgta
     9721 agtccaagcg gcgggggcga cgttgttttt catcgccgca ttttccgcag gcataccgaa
     9781 cgcgtcccaa cccataggtt gcatgacgtt gaagccgttt aagcgtttga agcggctcaa
     9841 tacgtcgccg atggtgtagt tgcgcacatg ccccatgtgc agcttgccgc tggggtaggg
     9901 gaacatggag aggcagtagt atttgggttt ggaagcgtct tcggagacgt tgaaaatacg
     9961 ggcgtcgtcc cattttttct gcgccgcagg ctcgatggcg gcgggccggt aatgttcttg
    10021 catggtcatt ctgtttttcg cttgaaaacg ttaggaattt aaaggaggaa ttataacagg
    10081 ttgccgatgt cggcgaatcg gcaaattgcc ggcaggatgc gtaaattcgc acgcgcatca
    10141 ttccgtatgc cgtacaaata caccgcgttt actgatacgc acgtttttta tgctaatatt
    10201 acaaaccaaa atcaaatgtt taaaactctc ctgatgcggc tcttccgaac aaaaggcaga
    10261 cgggcatcgg gtaaaagagg attctgcata tgaaaatcaa acaaatcgtc aaaccgggct
    10321 tggcagtatt ggcggcgggc gttctgtctg cctgcgcaac caaaagcaac gtcaaagccg
    10381 acggaacgac cgacaatccg gttttcccga aaccctattc cgtaacgctc gacaacaatc
    10441 gcggtacatt cccgacctat gacgaattgg acttgatgcg tcccggtctg accaaagacg
    10501 acatctacaa aatcctgggt cgcccgcatt acgacgaagg tatgtacggc gtgcgcgaat
    10561 gggattacct gttccacttc cataccccgg gcgtaggtat cgaccctgaa aacacttccg
    10621 gcgtagaaga tgttactacc tgccaataca aagtgatttt cgataaagac aaatttgccc
    10681 gcagcttcta ctggaacccc gtcttcccga aagatgccgc ctgtccgccg cccgcaccca
    10741 aagccgagcc gcaagtcatc atccgcgaaa tcgtgccggc aaaaccgaaa cgtatccgcc
    10801 aataatacgc aatgccgccc cgcctgcttt tagggatatt gtgctgcctg ccgatttttg
    10861 cccccgcgca cgcgcagggg cagccggata cggtcggcga ctttatccaa aagaaaaaag
    10921 tcatcgtcga tacatccaaa gcggaactct gttttgccga tgacaggcag tgccaccccg
    10981 tcctcatcgg tgttgccacg cccaagggga cgttcgggct gacgctgaac agtaccgaca
    11041 agcccggata cggcggcgaa gtcatcggtt tcaagcagga gggtgatttt cttttcgccc
    11101 tgcaccgcgt ttggaatcag ataccgtcgg aaaggcggaa cgaacgcatc gcctccccgt
    11161 ccgtgtccga caggattatg accaacggct gcatcaacgt cagcgatgcg gtgtacgaaa
    11221 aactgcgtca ttattttgtg ttggaagtga tttgaaacag acggataccg cacgcgccgg
    11281 tatctgtttt cacattgccc cgatgcctga aacagactgt ccgccacgtc atgccgtctg
    11341 aaaccggcgc agatgccgcc aagccttcag acggcattgc ctgcccgctc cgaccgaaca
    11401 acaaccatct ttgggagaac cttatgcccg aacaaaaccg catcctctgc cgcgaactaa
    11461 gcctgctggc attcaaccgc cgcgtgttgg cacaggcgga agacaaaaac gtcccccttt
    11521 tggaacgcct gcgcttcctg tgcatcgttt catccaacct cgacgagttt ttcgaagtcc
    11581 gtatggcgtg gctgaagcgc gaacacaaac gctgcccgca gcgcaggctg gacaacggca
    11641 aaatgccgtc tgaaaccatc gccgacgtta ccgaagcggc gcgctccctg atacggcacc
    11701 agtacgacct gttcaacaac gtccttcagc ccgagctggc acaagaaggc atccattttt
    11761 accgccgccg caactggacg ggcacgcaga aaaaatggat cgaagactat ttcgaccgcg
    11821 aactgctgcc gatcctgacc cccatcggac tcgacccgtc ccaccccttc ccgcgaccgc
    11881 tgaacaaatc gctcaacttc gccgtcgaac tcgacggcac ggacgcattc ggcaggcctt
    11941 cggggatggc gattgtgcag gcaccgcgca tcctgccgcg cgtcgttccc ctgccgtccg
    12001 aactgtgtgg cggcggacac ggcttcgtct tcctgtcgtc catcctccac gcccacgtcg
    12061 gcaaactctt cccgggcatg aacgtcaaag gctgccacca gttccgcctg acgcgcgaca
    12121 gcgacttgac cgttgacgaa gaagacctgc aaaacctccg cgccgccatc caaaacgaac
    12181 tgcacgaccg cgaatacggc gacggcgtgc ggctcgaagt cgccgacacc tgtcccgcct
    12241 acatccgcga ctttctgctc gcgcaattca aactgaccgc cgccgaactc tatcaggtca
    12301 aaggcccggt caacctcgtg cgcctcaacg ccgtccccga cctagtcaac cgccccgatt
    12361 tgaaatttcc cacacacacg ccgggcagac tgaaagcctt gggcaaaacc gcgtccatat
    12421 tcgatttggt gcgccaatcg cccatcctgc tgcaccaccc ctaccaatcg ttcgaccccg
    12481 ttgtcgaaat gatgcgcgaa gctgccgccg accccgacgt gcttgccgtc aaaatgacga
    12541 tttaccgcac cggcacgcgt tccgaactcg tccgcgccct gatgaaggcg gcactcgccg
    12601 gcaaacaagt aaccgtcgtc gtcgaactga tggcgcgttt tgacgaagcc aacaacgtca
    12661 actgggcgaa gcagctcgaa gaggcgggcg cgcacgtcgt gtacggcgtg ttcggctaca
    12721 aagtccacgc caagatggca ctggtcatcc gccgcgaaga cggcgtactc aagcgttacg
    12781 cccacctcgg cacgggtaac taccaccaag gcacatcgcg catctacacc gacttcggca
    12841 tcattaccgc cgacgaacaa atcaccgccg atgtgaacac attgtttatg gaaatcacag
    12901 gcttgggcaa acccgggcgg ctgaacaaac tctaccaaag cccgtttacc ctgcacaaaa
    12961 tggttatcga ccgcatcgca cgcgaaaccg aacacgcaaa agccggcaaa ccggcgcgga
    13021 ttaccgccaa gatgaattcg ctcatcgaac cgaccgtcat cgaagccctg tatcgggcaa
    13081 gcgcggcagg cgtacaaatc gatttgattg tgcgcggtat gtgcaccttg cgcccgggtg
    13141 taaaaggctt gtccgaaaac atccgcgtcc gctccatcat cggcaggcag ctcgaacacg
    13201 cgcgcgtgta ttgcttccac aacaacggcg cggacgatac ctttatctcc agcgcggatt
    13261 ggatggggcg caacttcttc cgccgcatcg aaaccgccgc gccgattacc gcgcccgaac
    13321 tcaaaaaacg cgtcatccgc gaaggtttgg aaatggcatt ggcagacaat acgcacgcgt
    13381 ggctgatgca gcccgacggc ggctatatcc gcgccgcacc tgccgagggc gaatccgaag
    13441 ccgacctgca aaacgatttg tggacactgc tcggaggctg acccgcaccg ccccaatcaa
    13501 aaaccatgcc gtctgaaacc tttccgtttc agacggcatg gttttacagc aatctaaaca
    13561 gggcggaccg gagtcaaaaa cacaccttcg ccatttccgc ataagcactt cccctatacg
    13621 ctcccaaccc caatccgccg cattccagac ggcattatag tggattaaa
//
LOCUS       DAXHSJ010000039        13665 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000039 DAXHSJ010000000
VERSION     DAXHSJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13665
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.039"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            409..5865
                     /gene="iga"
                     /locus_tag="ACFM8U_000840"
     CDS             409..5865
                     /gene="iga"
                     /locus_tag="ACFM8U_000840"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC6334106.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYKPHKAWNASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRVGAGRQLVYEKGAYHQEGKEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADEIKQRDNAGTIKG
                     YGEHHWKTTGTNSHIGSTAVRLAGNERGANNGQNVTFENNGTLVLDQNINQGAGGLFF
                     KGDYTVKGINNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQG
                     QLKVGDGTVILNQKADSNQKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRL
                     DANGNDLTFEHIRNVDEGARIVNHNTGHTSTITLTGKSLITNPNSLSVHSIQNDYDED
                     DYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEE
                     AKKNAMNHKNNQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKI
                     SVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQ
                     SASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKALN
                     SFGATQINGNVNLSQNAALTLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLS
                     LADSHIHLNNASDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHY
                     QLHVQDKTGEPNQEGLNLFDASSVRDRSHLSVSLANNHVDLGALRYTIKTENGITRLY
                     NPYAENRRRVKPAPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAK
                     AEQVKRQQAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAA
                     ELSAKQKVEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEE
                     RRQTAQSQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEE
                     RKAAELLAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKA
                     AAEAQALAARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQE
                     ARQTAELARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKR
                     RAAPQDYMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPL
                     DALEDENVSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQ
                     DNTELHEKVETVSLQPRAAQPRAQAAAQPQAQADAVSTNTNSALSDAMASTQSILLDT
                     GASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRSLSEN
                     MQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQ
                     QKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVK
                     TLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFAYKADNQQQYSAGAA
                     LLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(6043..7245)
                     /gene="trpB"
                     /locus_tag="ACFM8U_000841"
     CDS             complement(6043..7245)
                     /gene="trpB"
                     /locus_tag="ACFM8U_000841"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC6334107.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTAAK
                     DEEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(7328..8017)
                     /locus_tag="ACFM8U_000842"
     CDS             complement(7328..8017)
                     /locus_tag="ACFM8U_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6334108.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAQMPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAVYRAF
                     FEMAGLCEKQL"
     gene            8214..8993
                     /gene="rsmA"
                     /locus_tag="ACFM8U_000843"
     CDS             8214..8993
                     /gene="rsmA"
                     /locus_tag="ACFM8U_000843"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC6334109.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINSQDRAEHIAPEKYVALSNYLAGKAV"
     gene            9166..9894
                     /locus_tag="ACFM8U_000844"
     CDS             9166..9894
                     /locus_tag="ACFM8U_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6334110.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVLFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            9967..10326
                     /locus_tag="ACFM8U_000845"
     CDS             9967..10326
                     /locus_tag="ACFM8U_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334111.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            10331..10771
                     /locus_tag="ACFM8U_000846"
     CDS             10331..10771
                     /locus_tag="ACFM8U_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC6334112.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGWMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGTTVSQCIIPDMTDKESYRRLKVWILWRQPKKTAETDTPS"
     gene            10803..12077
                     /gene="folC"
                     /locus_tag="ACFM8U_000847"
     CDS             10803..12077
                     /gene="folC"
                     /locus_tag="ACFM8U_000847"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC6334113.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARSEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVSFEKAGVFRSGKPAICGQNPAPESLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACTLTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            12090..13097
                     /gene="ftsN"
                     /locus_tag="ACFM8U_000848"
     CDS             12090..13097
                     /gene="ftsN"
                     /locus_tag="ACFM8U_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6334114.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSEKSGKKAAIQAGYAEKERA
                     LSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTN
                     E"
     gene            13090..13587
                     /locus_tag="ACFM8U_000849"
     CDS             13090..13587
                     /locus_tag="ACFM8U_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC6334115.1"
                     /translation="MNNLPVADILIFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAASFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIITLLVMLASKTDLPDTEEWQQSYTIPFFVSLSEAVLKHADDRDG
                     ALEDD"
BASE COUNT         3984 a         3600 c         3308 g         2773 t
ORIGIN      
        1 gaatccatcc gtacggaaac ctgcaccgcg tcattcccac gaacccacat cccgtcatta
       61 ccacgaaagc gggaatctag gacgcagggt taagaaaacc tacatcccgt cattccctca
      121 aaaacagaaa accaaaatca gaaacctaaa atcccgtcat tcccgcacaa gcgggaatcc
      181 aaaaacaaaa agccgcagaa atttaacgga agaaccgaac cccgaaaacc ggctccccgc
      241 tttcgcttga ataacagaac aaaggaagcg gaaataaaaa catcgggatt ctttttttaa
      301 attggcaata aaacaccaaa atgaatgatt tacacaacaa aaaatagtca acaccaccaa
      361 accggcgtaa aatacaaaac actatcactc ttaaaacggt aaaaccttat gaaaaccaaa
      421 cgttttaaaa ttaacgccat atccttatcc atctttcttg cctatgccct tacgccatac
      481 tcagaagcgg cattggtcag agacgatgtc gattatcaaa tattccgtga ctttgcagaa
      541 aacaaaggca aattttttgt cggcgcaacc gatttatcag tgaaaaacaa acaaggtcaa
      601 aacatcggca acgcattatc caacgtaccg atgattgatt ttagcgttgc agatgtcaac
      661 aaacgcatag caacggtagt cgatccccaa tatgccgtca gcgtcaaaca cgccaaagca
      721 gaagtccata ctttttatta cggtcaatac aacggtcata atgatgtagc cgacaaagaa
      781 aatgaatacc gcgttgtcga acaaaataac tataaacccc ataaagcttg gaatgcgagc
      841 aacttaggcc gtctcgaaga ttataatatg gcgcgtttta acaagtttgt aactgaagtt
      901 gcgccaattg caccaactga tgccggcggc ggattggata cctacaaaga taaaaaccgt
      961 ttttcctctt ttgtcagagt tggcgcaggc aggcaattag tttatgaaaa aggggcttat
     1021 catcaagaag gaaaggaaaa aggctacgat ttgcgcgatc tttcacaagc ctatcgttat
     1081 gccattgcag gtacacctta taaagatatt aatattgacc aaacaatgaa taccgaaggt
     1141 ttgattggct ttggtaatca taataaacag tattcggcag aagaacttaa acaagcactc
     1201 tcgcaagatg cgttaacaaa ttacggcgta ttgggcgata gcggctctcc gttatttgct
     1261 tttgacaaac aaaaaaatca atgggtcttt ttgggaactt atgattattg ggcaggttac
     1321 ggaaaaaaat cttggcaaga atggaatatc tataaaaaag aatttgcaga tgaaatcaaa
     1381 caacgcgata acgccggcac catcaaaggt tatggcgaac atcattggaa aaccacgggc
     1441 acaaacagcc atatcggttc gacagcggta aggcttgccg gcaatgaaag gggtgcaaac
     1501 aacggacaga atgttacctt tgaaaacaac ggcactttgg tattggatca aaacatcaac
     1561 caaggcgcgg gcggtctgtt tttcaaaggc gattacacag tcaaaggtat aaataatgac
     1621 atcacttggc taggtgcggg gattgatgtt gccgacggca aaaaagtcgt ttggcaagtc
     1681 aaaaatccga atggcgacag attggcgaaa atcggcaaag gcacattaga aataaacggc
     1741 acaggcgtta accaagggca attaaaagtc ggcgacggta cggttattct gaatcaaaaa
     1801 gccgattcaa accaaaaagt ccaggctttc tcccaagtcg gcattgtcag cggacgcggt
     1861 acattggtat taaatagttc aaatcagatt aatcccgata acctatattt cggtttccgt
     1921 ggcggtcgtt tggatgccaa tggcaatgac ttgacttttg aacacatccg caacgtggat
     1981 gaaggcgcgc gcattgtcaa ccacaacaca ggccacacct ccacaatcac gctaacgggt
     2041 aaatctttaa ttaccaatcc gaacagcctt tcggtacata gtatacaaaa tgactatgat
     2101 gaagatgatt atagttatta ttaccgacca aggcgaccca tcccacaagg caaagatctt
     2161 tattacaaaa actatcgtta ttacgcccta aaatccggcg gcagcgtgaa cgcaccgatg
     2221 cccgaaaacg gacaaacgga aaataacgac tggattttaa tgggcagcac acaagaagaa
     2281 gccaagaaaa acgcgatgaa ccacaaaaac aatcagcgta ttagcggttt tagcggtttc
     2341 tttggcgaag aaaacggaaa aggacataac ggtgcattaa accttaattt caacggcaaa
     2401 agcgcgcaaa accgtttctt gttaacaggc ggcactaatt taaacggaaa aataagcgta
     2461 actcaaggca atgtcttgtt atcaggtcgt ccaacaccac acgcaagaga ttttgtgaac
     2521 aaatcttcag cccgaaaaga cgcacatttc tccaaaaaca atgaagtcgt atttgaagac
     2581 gactggataa accgcacatt caaagccaca gaaattgcgg ttaatcaatc cgcatcattc
     2641 tcttccggca gaaatgtatc cgacatcacc gccaacatca ccgcaacaga caacgccaaa
     2701 gtaaatttgg gttacaaaaa cggcgatgag gtttgcgtgc gctcggacta taccggttac
     2761 gttacctgca acacagacaa cttatccgat aaggctttaa acagctttgg agcgacacag
     2821 attaacggca atgtgaattt gagtcagaat gcggcattga ctttgggcaa ggctgcatta
     2881 tgggggcaaa ttcaagggca aggaaacagc cgtgtcagcc taaatcaaca tagcaaatgg
     2941 catttgaccg gcgacagcca agtacacaat ctgtcattgg cggatagcca tattcatttg
     3001 aacaacgctt ccgacgcgca aagtgcaaat aaataccaca cgctcaaaat caatcattta
     3061 tccggtaacg ggcattttca ttatctgacg cacttggcaa aaaatcttgg ggataaagtg
     3121 cttgtgaagg aatccgcatc cggtcattat cagctccatg ttcaagataa aacaggcgaa
     3181 cccaatcagg aagggctgaa tctctttgat gcatcatccg tacgagaccg atcgcacctt
     3241 tccgtttcct tggcgaataa ccatgtagat ttaggcgcat tgcgttatac aatcaaaaca
     3301 gaaaatggca ttacccgatt gtacaatcct tatgccgaga accgccgccg agtcaaaccg
     3361 gccccgtctc ctgccacaaa cacggcttct caagcacaaa cggacagtgc acaaattgcc
     3421 aagcctcaaa atatcgtcgt cgcaccgcct agcccgcagg caaatcaagc cgaagaagca
     3481 aaacgccaac aagcaaaagc ggagcaagtg aagcgtcagc aagcagaagc agagcgtaaa
     3541 tcggcagagt tagcaaaaca aaaagcagaa gccgaacgcg aagcaagaga gctggcgacc
     3601 cgccaaaaag cagaacaaga acgcagcagc gccgaacttg cacgtcgaca tgaaaaagaa
     3661 cgcgaagcgg cagagttgtc ggcaaaacaa aaagtcgaag ccgaacgtga ggctcaagca
     3721 ttggcggtac gccgaaaagc agaagccgaa gaggcaaaac gccaagctgc agagcttgca
     3781 cgccggcatg aaaaagaacg cgaagcggca gagttgtcgg caaaacaaag ggtcggggaa
     3841 gaggaacgcc gtcaaacagc ccaatcccag ccgcaacgcc gcaaacgccg tgccgcaccg
     3901 caggattata tggcagcttc ccaagaccgt cctaaacgcc gcggacaccg ttcggtacag
     3961 caaaacaatg tggaaattgc ccaagcccaa gcagaacttg cgcgccgaca gcaggaagaa
     4021 cgcaaagcgg cagagttgtt ggcaaaacaa agggcagaag ccgaacgtga ggctcaagca
     4081 ttggctgcgc gccgaaaagc agaagccgaa gaggcaaaac gccaagctgc cgaacttgca
     4141 catcgccagg aagcagaacg caaagcggca gaattgtcgg caaaccaaaa agccgcagct
     4201 gaagcccaag cattggcggc acgccaacaa aaagcccttg cgcgtcaaca ggaagaagca
     4261 cgaaaagctg cagagttagc cgtcaaacaa aaagcggaaa ccgaacgcaa aaccgccgaa
     4321 cttgccaagc aaagggcagc agccgaagca gcaaaacgcc agcaagaagc tcgccagacc
     4381 gccgaacttg cacgtcgtca agaagcggaa cgccaagcag cagagttgtc ggcaaaacaa
     4441 aaagccgaaa cagatcgaga agcggcagaa tccgcgaaac gaaaagctga ggaagaagag
     4501 caccgtcaag cagcccaatc ccagccgcaa cgccgaaaac gccgtgccgc acctcaggat
     4561 tatatggcag cttcccaaaa tcgtccgaaa cgtcgcggac gcagatctac tctgccggca
     4621 ccgccctcgc catcatttga ttcgtcagct tacgcagcac ccagggcctt gcataatccg
     4681 gactggtatg aaaatgacta tgaagaaatc cccttggatg cgttggaaga tgaaaatgta
     4741 tctgaatcag ttgacacatc agacaaacag cctcaagaca atacggaact tcatgaaaaa
     4801 tatgaaaatg attatgaaga aatccccttg gatgcgttgg aagatgaaga tgtatccgaa
     4861 tcggttgaca catcagacaa acagcctcaa gacaatacgg aacttcatga aaaagttgag
     4921 acagtgagtt tgcaaccaag agccgctcag ccgcgagccc aagccgccgc gcagccgcaa
     4981 gcccaagccg atgcagtcag caccaatacc aactcggctt tatctgacgc aatggcaagc
     5041 acgcaatcta tcttgttgga tacaggtgct tcattaacac ggcacattgc acaaaaatca
     5101 cgtgctgatg ccgaaaaaaa cagcgtttgg atgtcgaaca tcggttatgg ccgtgattat
     5161 gcttccgcac aatatcgccg gtttagttcg aaacgcacgc aaacacaaat cggcattgac
     5221 cgcagcttgt ccgaaaatat gcagataggc ggcgtattga cttactctga cagtcagcat
     5281 acttttgatc aggcgagcgg caaaaatact tttgtgcaag ccaaccttta tggtaagtat
     5341 tatttaaatg atgcttggta tgtggccggc gatattggtg caggcagctt gagaagccgg
     5401 ttacaaacgc agcaaaaagc aaactttaac cgaacaagca tccaaaccgg ccttactttg
     5461 ggcaatacgc tgaaaatcaa tcaattcgag attgtcccca gtgcgggaat ccgttacagc
     5521 cgcctgtcat ctgcagatta caaattgggt aacgacagtg ttaaagtaag ttctatgtca
     5581 gtgaaaacac tgacggccgg actggatttt gcttatcggt ttaaagtcgg caaccttacc
     5641 gtaaaaccct tgttatctgc agcttacttt gccaattatg gcaaaggcgg cgtgaatgtg
     5701 ggcggtaatt ccttcgccta taaagcagat aatcaacagc aatattcagc aggtgccgcg
     5761 ttactgtacc gtaatgttac attaaacgta aatggcagta ttacaaaagg caaacaattg
     5821 gaaaaacaaa aatccggaca aattaaaata cagattcgtt tctaaaatac taaattcata
     5881 gcaaaataaa atgccgtctg aactcaggct tcagacggca tttttatagt ggattaacaa
     5941 aaatcaggac aaggcgacga agccgcagac agtacagata gacggcaagg cgaggcaacg
     6001 ccgtactggt ttttgttaat ccactatatc agacggacga ggttacagtt taatcccttt
     6061 gagcttcgcg acggtattga tgtctttgtc gccacgacct gagaggttga ccagaatcac
     6121 ttggtcttta cccattttcg gcgcgttttt caccgcccaa gcaacggcgt ggctggattc
     6181 gagcgcgggg atgattccct cgaagcggca gagcaaatca aaggcttcga gtgcttcttc
     6241 gtctttggcg gcggtgtatt cgacgcgctt gatgtcgtgc agatggctgt gttccgggcc
     6301 gatgccgggg taatccaagc ctgcggaaac agagtgcgta cccaaaacct gaccgttttc
     6361 gtcctgcatc agatagctgc ggaaaccgtg cagtacgcca atcggtgcgc ccgaagtaat
     6421 cggcgcggcg tgatcaggag tgttcacgcc caaaccgccg gcctccacgc cgacgaggcg
     6481 cacgttttct tcgccgatat acgggtggaa caaaccgatg gcgttcgatc cgccgcccac
     6541 gcaggcaacg gcaacgtcgg gctgtctgcc gatggcttcc aacatttgtt ctttggcttc
     6601 attgccgatc acgcattgaa agtcgcgcac catttcggga tacggcgcgg gaccggcggc
     6661 ggtaccgatg atgtagaatg tgtcatccac gcgggcgacc cattcgcgca tggcttcgtt
     6721 catcgcgtct ttcaaagtac ggctgccgct gtcaacgccg acgacattcg cgcccaataa
     6781 tttcatacgg aacacgttgg gcatttggcg ttggatgtcg tccgcaccca tgtacacgtc
     6841 gcaagtcatg ccgaagcggg cggcaacagt ggcactcgcc acgccgtgct gacccgcgcc
     6901 ggtttcggcg atgacgcgtt ttttacccat acgttttgcc aacagtgcct gaccgatggt
     6961 gttgttgact ttgtgcgcgc cggtgtggtt caagtcttcg cgcttcaacc agatttgcgc
     7021 accgcccaaa tgctcggaca accgcgcggc gtggtaaacg gggctgggcc tgccgacata
     7081 atgtttcaaa tcgcggtgga attcttccca aaacgacggg tcgtttttcg ctgccttata
     7141 ggcatccgcc agctcttgca aagcgggaat cagggtctcg gacacgaaca gcccgccgtg
     7201 ttcgccgaaa aagcccttct cgtcgggcgc gtggtagttc ttcatcggat tcttccttgt
     7261 gcttcaggtt ttcaaacggc atttccgttt tttcaaacat gccgtccgaa cgggaaattg
     7321 tcggcaatta taactgcttt tcgcacaagc ccgccatttc gaaaaacgcc cgatacaccg
     7381 ccgccttttt cgccaaactc aaaggatagg cgcgcgaagt cgaaggcagg cgcgtcagcg
     7441 tcaaatcccg tccggcaaac ggaaacggca cggtttcgcc cgttttcggc attttaatgc
     7501 cgccgccctg aatatcgagc aggatttccg tcgccttgcc gcctgtcgta caaatatggc
     7561 ggcactcggg catctgcgcc aacaccgccg ccaaatcgac ggtttcaact acctttaaaa
     7621 acttgtcgga cgcattgccg tgttcccttg ccgccttcaa cacggtcggg caggacgcaa
     7681 tcccccgttc gtgcaaaaac gccttgattt tctcggcatc aaacgctttt tcagacaacc
     7741 tttggaaatg cgccgcatca ttaaaaaaca ccagcccgta aacgcgccac atatcgtttt
     7801 ggaaattcgg ataatgaaac tgcatcgcgc gtttgtcttc cttgggcgga aacgtcccca
     7861 tcatcatgac cgcagccttc ggcggcaaca cggaatcgaa gggatgggtt tcgatttcaa
     7921 gcagattttc agacacgttc aatcctccaa caggcaaaac ggcagacagc cggtttttgc
     7981 accaaaccgc caaacacggc aagccttcaa acagcattat cacgccctgc cgttttcggt
     8041 tacaatgccg acatcaaacg gatactgtaa acacatttat gttccaacac acaggacgac
     8101 acttaaagca ccgccctatg tgccatcctg atttggaagg agttacgccc ctcccaaata
     8161 gagtctgatt ctaccgccct aaagggcggg gtttcaaccg aaaaggaaac acgatgaaag
     8221 aacacaaagc ccgcaagcgt ttcgggcaga attttttgca ggacacgcgg atcatcagcg
     8281 atattgtcaa cgccgtgcgc ccgcaggcgg atgatgtcgt gattgaaatc ggcccgggtt
     8341 tggcggcgat taccgaacct ttggcgaaaa agctgaaccg cctgcacgtt gtcgaaatcg
     8401 accgcgacat cgtatgccgt ctgaaaacgc tgccgtttgc ggataaactg gtgattcacg
     8461 aaggcgatgt attgcagttt gatttcaacg gcatcgcagg caaaaagaaa atcgtcggca
     8521 acctcccgta caacatttcc acaccgcttt tgttcaagct ggcggaggtg gcggacgatg
     8581 tcgtcgatat gcactttatg ctgcaaaaag aagtggtcga gcgtatggtt gccgcgccga
     8641 aaagcaacga ctacgggcgt ttgggcgtga tgctgcaata ttttttcgat atggaaatgc
     8701 tgattgacgt accgcccgaa tcgttcgacc ctgcgccgaa agtggattcc gcagtggtgc
     8761 gcatgattcc ggtgaaacac cgcatcggca aagcggacga tttcgagcat ttcgccaaac
     8821 tcgtaaaact cgccttccac caacgccgca aaaccatacg caacaatctg aaagagcttg
     8881 caggcgacga tgatttgcag gcagtcggca tcaattcgca agaccgcgcc gaacacatcg
     8941 cgccggaaaa atacgtagcg ttgagcaatt atttggcggg caaggccgtc tgaaaaggga
     9001 gtaagccgat gacgaaatac ctgcttttac ccctgatact gccgcttgca gcctgcctga
     9061 ccaatccgaa cgaccgtttt tttgacggca gacgctacac gcttcatcat atggagctcc
     9121 cgtaacgaca acctgcaacg gcaaatgccg tctgaaagaa aagacatgat caaattcaaa
     9181 aacgtacaca aacatttcaa agacctgcac gtcatcaacg gcgtgaactt ggaaatcaaa
     9241 aaaggcgaag tggtcgtcgt ctgcggacct tcgggcagcg gcaaatccac actcatccgc
     9301 accgtcaacc agcttgaaag catcgaaagc ggcgagattt gggtggacgg tgtcaacgtt
     9361 gccgacccaa aaaccgattt gaacaaaatc cgcgaagaag tcggctttgt gtttcaaagt
     9421 ttcaacctct acccgcacct gaccgtgttg gaaaacatta ccctcgcacc gatgaaggtt
     9481 aaaggtcaaa atgcggaaca ggcagagaaa aaagcgatgg aacttttgga acgcgtcgga
     9541 ctggcacaca aaaaagacgc cttcccttcc caactttccg gcggccagca gcaacgcgtg
     9601 gcgattgcac gcggtttggc gatggaaccg cgcgtaatgt tgtttgacga accgacctcc
     9661 gcactcgacc ccgaaatggt cggcgaagtg ttgaaagtga tgaaagactt ggcggaaagc
     9721 ggtatgacga tgatgtgcgt aacccacgaa atgggttttg cccgcgaagt tgccgaccga
     9781 gtgctttttg tcgataaagg gcaaatcctc gaagacgaaa cgcccgaagc gttttttacc
     9841 aacccgaaac acgaacgcgc caaacaattc ctgcaacagg tcatgaccca ctgatgctat
     9901 gccgtctgaa aaccgaacgt tcagacggca tagcccgaca ccgcaagatg attgagaaag
     9961 aaaaccatgt attttgttga ccgcaccgcc gtcgtcctca agccgaccgt ccgctttttg
    10021 gaatggctca aaagcaccga cgaaaatatg cccgacctga ccatagaaca gcttcgtgcc
    10081 aactgttccg tcttcctcgt tccgcagttc gacgaaccgg aagccgtcgt ttcctatttc
    10141 gacgaacgct accgccagat tttcgaggcg gagctggcag gttgggacat cgataaggat
    10201 aaatggccgc aagatatggg gctgaaggcg ttttgggaat ttttcgacat cgaaatccac
    10261 gatatggtgt tggacatgga agaagcggaa ttgaacatta cccccgtgtt cgacaacatg
    10321 atgtaaggct gtgcgcgcct ttcagacggc attgaggccg tcccgcatcc tgaacatcct
    10381 gactgtatcg cttcacgccg cctcccttgc cgtctgcctg acttggtttt acggctggat
    10441 gatgtggttc ggtttggcgg ctttggtggc aagttacgca tactcgctca ggataacgaa
    10501 tctgaaacac cgccatgcca taaccgccat taccatagac cgcgacggac aggcggaaat
    10561 cgtatccggc aaagacaaaa cagcggcaac acttgcgggc agcagtatgg ttacgcctta
    10621 tgccttattc ttacaatggg acacaggcgg cacaaccgtc agccaatgca tcatacccga
    10681 catgacggac aaagaatcct accggcggct gaaagtctgg atactttggc ggcagccgaa
    10741 aaaaaccgcc gaaaccgata caccaagctg atttcagacg gcatttgaac acggaacact
    10801 caatgaaaac actgcaagac tggctctcgc atttggaaac cgcccacagc ggcggcttga
    10861 tcgatatggg cttggaacgc gtaggcgaag tgaaaaaacg gatgaagctc acgccgcaat
    10921 gccccgtcgt cgttgtcgcg ggaacaaacg gcaaaggttc ggtctgcgcc tacctgacac
    10981 agatttacaa acaggcgggc tacaaaaccg gcacactgac cagcccgcat ttattgcgtt
    11041 acaacgaacg catcgccatc aacgccgaac ccgtttcgga cgatacgatt attgcctctt
    11101 tcgagcgcat cgaagcggcg cgcagcgaaa tatcgctgac ttattttgaa ttcaatacgt
    11161 tggcggccgt cgacatcttc atgcgcgaac aggttgacgt gatgatattg gaagtcggct
    11221 tgggcggacg cttggacgcg gtcaacgcat ttgacggcga ttgcgcggtg gttactagtg
    11281 tagatttgga ccatcaggcg tttttgggcg atacggtcga gcaggtcagc tttgaaaaag
    11341 caggcgtgtt ccgcagcggc aaacccgcaa tctgcggtca aaaccccgcg cccgaatcgt
    11401 tgctttcaca cgccgaagcc ataggcgcga aactgctgat ggtgcagcgc gatttcgagt
    11461 ttcacgcgat ggaaaacatc caatggaact accgcttccg gccgcaacat tcagacgacc
    11521 ccgcgcgcaa ccgcaatgcc ctgcccttcc ccgcattgcg cggcacatac caactttcca
    11581 acgccgcctg cacgttgacc gtattggaat gcttgaacga caaattgccg gtggacatcg
    11641 gcgccatcaa gcgcggtttg ttgctggttg aaaaccccgg ccgcttccaa gtcctgcccg
    11701 gccgtccgct gaccgttttg gatgtcggac acaatcccca cgctgcccgc gccctgcgcc
    11761 gcagcctgat taatttggcg tttgcgcaaa aacgcaccgc cgtattcagt atgctgtccg
    11821 acaaagacat agacggcgtg ttggaaatcg ttaaagacca gtttgacgag tggtatatcg
    11881 caccgttaga cgtaccgcgc ggcatgacgc tcgagagcct gcaaaacaaa ctgcacgaac
    11941 acagcatcga aaacatacag acattcgccg ccgtccgcga agcataccgg gcggcagcct
    12001 caaaagcggg cgagaacgac agaatcgtcg ttttcggctc atttcatacc gttgccgatg
    12061 tcatgtcggt actttaaaag gaaacttcta tgtccgaaaa caaacaaaac gaagtcctga
    12121 gcggttacga acaactcaaa cggcgcaacc gccgccgcct cgtaacggca agttgcctgg
    12181 ttgccgcctc ctgcatcctg ctggcagccg ccctcagttc cggccctgcc gaacagactg
    12241 ccggcgaaac aagcggcgta gaaaacaaag cggcaggtgc ggcacaaacc cctgccttga
    12301 aatccgccgc cgacaaaccg caggacttgg caggcgaaga caagccttct gccgccgaca
    12361 gcgaaatcag cgagcctgaa aacgtaggcg cgccgctggt gctgattaac gaccgcctcg
    12421 aagacagcaa catcaaaggt ttggaagcat ccgagaaact gcaacaggca gaaaccgcca
    12481 aaaccgcacc gaagcaggca aaacaacgcg ctgccgaaaa agtgccggca actgccgaca
    12541 gtacggatac ggtagcggtt gaaaaaccga aacgcactgc cgaaacaaaa ccgcaaaaag
    12601 cggaacgcac tgccaaagcc aagcccaaag ccaaagaaac caaaaccgcc gaaaaagttg
    12661 ccgacaaacc gaaaactgcc gccgaaaaaa ccaaaccgga tacggcaaaa tccgacagcg
    12721 cggtaaaaga agcgaaaaaa gccgacaagg ctgaaagcaa aaaaacagcc gaaaaagacc
    12781 gttcggacgg caaaaaacac gaaacggcac aaaaaaccga caaagcggac aagaccaaaa
    12841 ccgccgaaaa agaaaaatcc gaaaaatccg gcaaaaaagc cgccattcag gcaggttatg
    12901 ccgaaaaaga acgcgccttg agcctccagc gcaaaatgaa ggcggcgggt atcgattcga
    12961 ccattaccga aattatgacc gacaacggca aagtttaccg cgtcaaatca agcaactata
    13021 aaaacgcgag agatgccgag cgcgatttga acaaattgcg cgtacacggc atcgccggtc
    13081 aggtaacgaa tgaataacct ccccgttgcc gacatactca tcttcgccat catcgccgcc
    13141 tgcatcgtgc tatccgcgat gcgcggcgtg attgcggagg caggttcgat ggcggcatgg
    13201 gtggtttcct tctttttcgc caaactcttt gccgcctcct tcgccgacct cgcctttgcc
    13261 tcgttccaac cccgcctgtt tgcattggct ctgtcgttca tttccctgtt cgtcattgcc
    13321 tgtctgatcc agaaaatgct ccgttcgctg ctgaccggcg cagtttcggc ggtcggtttg
    13381 ggctttgcca accgcatttt gggcggcgta ttcggtgcat tgaaaggcgt tttgattatt
    13441 accctgctgg tcatgcttgc ttcaaaaacc gacctgcccg ataccgaaga atggcagcag
    13501 tcctatacca taccgttttt cgtgtcgctt tccgaagcag tgttaaagca tgcggacgac
    13561 agggatggag ctttggaaga cgattgattg ccatacggat atttcagacg gcatcctctt
    13621 ttatgccgtc tgaaatgttt tttcctttcc tcagccatat cacag
//
LOCUS       DAXHSJ010000040        13555 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000040 DAXHSJ010000000
VERSION     DAXHSJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.040"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(235..570)
                     /locus_tag="ACFM8U_000850"
     CDS             complement(235..570)
                     /locus_tag="ACFM8U_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC6334116.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(650..2779)
                     /gene="dnaX"
                     /locus_tag="ACFM8U_000851"
     CDS             complement(650..2779)
                     /gene="dnaX"
                     /locus_tag="ACFM8U_000851"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC6334117.1"
                     /translation="MDYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYAPPALQLLGRAAAGSMRDAL
                     SLLDQAIAMGSGSVAEQDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTIGGEQIQLYYQIAVHG
                     KRDLSLATDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETSAK
                     KPQPRPEAETAQTSVQTASAAAMPSESKTAEPVTNQENNDVPPWEDAPDEAQTAACTA
                     QASAKSIQTASEAETPPENQVSKNKAADNVTDAPLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDGAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWHDEAGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1551..1590)
                     /gene="dnaX"
                     /locus_tag="ACFM8U_000851"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2874..4850)
                     /gene="mutL"
                     /locus_tag="ACFM8U_000852"
     CDS             complement(2874..4850)
                     /gene="mutL"
                     /locus_tag="ACFM8U_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC6334118.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIGLALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCTTMLERLALAHPHIAFSLKRDGKQVFKLPTQSLPERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRANLTE
                     SVGNAGEVLHDITGVVSTPMPSENDSENLFDSASDYPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMNTYAELYKKTDDIDLELNRLEQARFGNMPSETPIPKT
                     DTPLSDGLPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     RENGKLQSQRLLIPSTFAASHEECAALADHAEALAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLSELAQVGSSQTIEEHENHILATMSCHGSVRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4993..5178
                     /locus_tag="ACFM8U_000853"
     CDS             4993..5178
                     /locus_tag="ACFM8U_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC6334119.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5189..5857
                     /locus_tag="ACFM8U_000854"
     CDS             5189..5857
                     /locus_tag="ACFM8U_000854"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6334120.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTVLALALPKHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            5857..6573
                     /locus_tag="ACFM8U_000855"
     CDS             5857..6573
                     /locus_tag="ACFM8U_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC6334121.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGKIVRLSNEVETLNGKVKALEHAKTHPSGRTYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAAALLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQAYPGSPAAKRAASAIRKR"
     gene            6621..7106
                     /gene="purE"
                     /locus_tag="ACFM8U_000856"
     CDS             6621..7106
                     /gene="purE"
                     /locus_tag="ACFM8U_000856"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC6334122.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARERGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7289..8743)
                     /locus_tag="ACFM8U_000857"
     CDS             complement(7289..8743)
                     /locus_tag="ACFM8U_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC6334123.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8740..9441)
                     /locus_tag="ACFM8U_000858"
     CDS             complement(8740..9441)
                     /locus_tag="ACFM8U_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC6334124.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNINAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9438..10217)
                     /locus_tag="ACFM8U_000859"
     CDS             complement(9438..10217)
                     /locus_tag="ACFM8U_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC6334125.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            10501..11907
                     /locus_tag="ACFM8U_000860"
     CDS             10501..11907
                     /locus_tag="ACFM8U_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6334126.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIATDL
                     DESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGNDTALDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQIAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWVFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTLS"
     gene            12019..13545
                     /locus_tag="ACFM8U_000861"
     CDS             12019..13545
                     /locus_tag="ACFM8U_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC6334127.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVSEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
BASE COUNT         2735 a         3662 c         3815 g         3343 t
ORIGIN      
        1 tttcagacgg catttaagcg gtacggatgt gtaaataatg gtagggtggg cttcagcctg
       61 ccgcatccca tcgattctgc cgtttttacc gtttccgccg aatcctgcaa actgatgccg
      121 tcattcccgc gaaggcggga atctagaccc gtcggcacgg aaatttatcg agtaaaacgg
      181 tttcttagat tctacgttct agattcccgc ctgcgtggga atgacggtcg ggtatcagcg
      241 gaagaagtcg cccactccgg ggggtagacc ttgcgtgaat gcgcccattg ttttgtttgc
      301 ggtttcttcg gctttgcctc gggcggattt gagggcggcg aggatgaggt cttcgagcat
      361 ttctttgtcg tcggcggctt cttgaatcaa atcggggctg atgtcgattt tgcgtacttc
      421 gtgcgcgcag gtcattgtga ttttgaccag gccgttgcct gcttcgcctt cgatttcggt
      481 ttcggcaagt ttggcttgcg cttttttcat attttcctgc atttgctgcg cctgtttcat
      541 caggccgcct aatccggctt ttccgaacat aggtaaaact cctgtctgtg tttgaatgaa
      601 tggggaaacg gcaatgccgt ccgaaaggtt tgggcggcat tatatctgtt tatggccggt
      661 ttgccgctaa ttccagtgat tcgggctgcc attgcgcgcc gaatgcttgg aggatttttt
      721 gtgcggcggg gtcggcttcg agcaatgctt gtgctttttg cctgtcttcg gcttggacgc
      781 gcttgtcctg catcgcgggg gtttctctgc cggcttcgtc gtgccagtct tgggtttgca
      841 gggtgagttg cagcccgtag gcttgggcaa gggtgtcgcg gattttgtcg agacgttttt
      901 tgtcggcggt ggcgcgtgct tcggcggtca ttgccaaaac catcaggccg gtgtcgggat
      961 ggtattccgt ccacgcggaa tgttgcgccg gcatttgcgc cgcgccgagt ttgcgggcga
     1021 aatgccggac gatggctgcc cagttttcgg tggaaaattc gggcggcggg gcggacggcg
     1081 tgttgtttcc gccgatgccg cctgcttctg cttcttcgtc cgcgcctccg cctgcggtat
     1141 cggcaggggc ggcgtgttcc caatcgggcg gcgggatttc tgcgccgtct tctacggggc
     1201 agtcattatc cggaaagccg taaccgtaga aaggttctgc aggggcttcg tgtgcaaatg
     1261 tttccgtttc aacggcttca tcattcggtg ttgcctgaat ggggttttca gacggcactt
     1321 cggacaaggg ggcatcggtt acgttgtcgg ctgccttgtt cttggaaact tggttttcgg
     1381 gcggcgtttc ggcttcggat gccgtctgaa tgctttttgc cgatgcttgc gccgtgcagg
     1441 ctgcggtttg ggcttcgtcc ggcgcgtctt cccaaggcgg aacatcgttg ttttcttgat
     1501 tggtaacggg ttcggcggtt ttgctttcag acggcattgc cgctgcggat gccgtctgaa
     1561 cggatgtttg cgcggtttcc gcttcagggc gcggttgggg cttttttgcg gaggtttcct
     1621 tttcggcggt ttgtgccgat ggggattgaa gttcggtatt ttcaatcacg gcgtttgcat
     1681 cacacgaagc tgccgccaag ggcgcaaacg ccagcatacg cagcagggtc atcataaagc
     1741 cggcgtattc gtcggtggcg aggctcaggt cgcgtttgcc gtggacggcg atttggtagt
     1801 aaagctggat ttgttcgcca cctatggttt gggcgaggcg gtgcaaaata tcggaatcgg
     1861 ggtcgtcgtg cgccaaggca ttcggcactg cctgtatcag ggcgaggtgt tgcagcagta
     1921 tggcaagttc gcccaaggcg ttgtcaaagc cgacggcaca cgcagccatt tcctgtgctt
     1981 ttgccagcag ggcttcgccg tcttggttga cgatgcctgt cagcagttcg tacaggtatt
     2041 gtttatcgac cgcaccgatc atttggcgga cgtcttgttc ggcaacgctg cccgaaccca
     2101 tggcgatggc ttggtcgagc aggctcaagg catcgcgcat cgatccggcg gcggcgcgtc
     2161 ccaaaagttg cagggcgggg ggggcgtagg cgattttttc gctgtcgagg acgtgggcaa
     2221 ggtggtcggc aacctgttgc gcggtcatat tgcgtaagac gaattgcagg cagcggctca
     2281 agacggtaac ggggactttg tgcggatcgg tcgtggcaag gatgaatttg acgtgttcgg
     2341 gcggctcttc cagcgttttg agcatagcgt tgaacgcgct tttggaaagc atatgcactt
     2401 cgtcgatgat atagactttg tatttgccgg cagtcggcgc gtattgggcg ttttccaata
     2461 cttcgcggat gttgtcgatg cctgtgttgg aggcggcgtc gatttcgagc aggtcgacat
     2521 agcgtccggc atcgatctgc gtacagcttt cgcacacgcc gcaaggttcg ccgtgttgcg
     2581 cgttttcgca gttgaggctt ttggcaagga tgcgggcgat ggtggttttg cccacgccgc
     2641 gcgtaccggt cagcaggtag gcgtggtgca gcctgccttc gtccaaggca ttttgcaggg
     2701 ctttgacgac gtgttcctga ccgactaagt cggcaaaggt tttgggccgc cattttcggg
     2761 cgagaacttg atagtccatg tttttctctt ggtttcggtc gtgatgtttc tgtcggtgct
     2821 tcggaatgcc gtctgaacgg cggtctcggg cggcgtattc tagcactttc ggtttattgt
     2881 ccgcgcaaga acagtgcgtc caattctttc aaagtcagtt tgacccaagt cggcctgccg
     2941 tggttgcact ggttgctgcg cggcgtgttt tccatatcgc gcagcagcgc gttcatttcg
     3001 ggcagggtaa gccgacggcc ggcgcggacg gagccgtggc aggacatggt ggcgaggatg
     3061 tggttttcgt gttcttcgat ggtttggctg ctgccgactt gggcaagttc gctcaataca
     3121 tctttggcca gtgaaacgac gtcggcttta cctagcatgg cgggaactgc gcggacggca
     3181 agggtattgc cgcccatatc ggacagttcc agcccgaagc ctgccagtgc ttcggcgtgg
     3241 tcggcaaggg cggcgcattc ttcgtgggac gcggcaaagg ttgagggaat aagcaggcgt
     3301 tggctttgca gtttgccgtt ttcccgacgt tggcgtttca ttttttcgta gttgacgcgt
     3361 tcggcggcgg cgtgcatatc gatgagcaac aggctgtctt cggcttgggc aaggatgtag
     3421 atgcctagca attgggcaat ggcgaaaccg agcggcggca gttcggattg ggagggaaga
     3481 ccgtctgaaa gcggcgtatc tgtttttggg ataggcgttt cagacggcat attgccgaaa
     3541 cgcgcctgct ctaatcggtt caactcaagg tcgatgtcgt cggttttttt gtaaagctcg
     3601 gcgtaagtat tcattgccgc gcggctttcg cgcagggaca ggctgtgttg ttgcggcgca
     3661 cgcgcggctt ggtaaggcat gggcgcggtt ttgcctgatg aaccaaaggc attgcgtgta
     3721 tcgggtttgt tgcctgtcgg gtagtcggat gcgctatcaa acagattttc gctgtcgttt
     3781 tcagacggca ttggggtgga gacaacgccg gtaatgtcat gcaacacttc gcctgcgttg
     3841 ccgacgcttt cggtcaggtt ggcgcgtgtg tcggcaaggg ctttgttgag cgtgtggaac
     3901 acaagttggt gcacctgctg actgtcgcgg aagcggattt cggttttggt cgggtggacg
     3961 ttgacatcca cggcttcggg cggcaggtcg agaaagagga cgaaagcagg ggtaagggcg
     4021 ttgtgcaata cgtcgcggta tgcctgtttg acggcgtgga gcatcacttt gtcgcggaca
     4081 aaacgatggt tgacgaagca gtattgtttg tcagttttgc ctttggcaaa agtcggcttg
     4141 gcaattgcgc catagagccg caatgcgcca ttgccgctgt caatttccaa tgaagccgtc
     4201 tgaaaatcat cgccgacaat ggcggcaatc cgttcaggca agctttgggt tgggagtttg
     4261 aatacttgtt tgccgtcgcg tttgagtgag aaggcaatgt gaggatgcgc cagtgcaagg
     4321 cgttcgagca tggtggtgca gtgggcgtat tcggtgtttt cggatttgag gaacttgcgc
     4381 cgcgcagggg tattgaagaa gagttcggcg gcttcgatgg tggtgccgac ggggtgggcg
     4441 gctgccgtag ggctgctgag ttttccgtct tctgctttga cttgggtcgc gtgcgaactg
     4501 tcttcttgac ggctggtcag ggttaggcgg ctgactgagg cgatactggc caaaccttcg
     4561 ccgcgaaagc ccatgctggc aacgtgttcc aaatcgttta gagttttgat tttgctggtg
     4621 gcgtggcggt ggagcgcaag cccgatgtcg tctggatgga taccgctgcc gttgtcgctg
     4681 acgcggatga gacggatgcc gccgcccgcc agttcgacat caatcgccgt cgcgcctgcg
     4741 tctatgctgt tttcaacgat ttcttttaag gcgttggcgg ggcgttcgac cacttcgccg
     4801 gcggcgattt ggttgacaag atggtcgggc agggcggcaa tgcgtggcat aaggtaggct
     4861 tccgttgcaa aaacgcttta ttctataata aaacccctta cttttctgcc tgtattcttg
     4921 ataaaagccg tttatcccgt ttggaaaata ccagtataat cacgccgtat cccgtaaaat
     4981 tggagcgcaa agatgtatca ctaccaatcc gaagccacac aattcctcaa ccgtctgatt
     5041 gaagaaaaac ctgagttgga acagcagcgt ttggaaaacc gggggctttt gtgggatgtc
     5101 gaactcaatc ccgaagaaca ggaaaacttc gaggcggcaa aagtagcgaa aaaaccttat
     5161 acctattacc aagactgaac ccaccgaaat gacgacccat ctgtccaatg tcgcccccga
     5221 cctgcaaaac tatttgaacg ccatcggcga acccgaacat cccgttttga cgcggctgcg
     5281 tgagaagacc gagcatcacc gtatgggcaa aatggcgatt gcgcgcgaac aggcggcggt
     5341 tttggtttgg ctggcaaagc tgattcgcgc ggaaaaatat ctggaagtcg gcgtatttac
     5401 cggatacagc agcaccgtgc ttgcattggc gttgccaaaa cacgggcgga ttaccgcctg
     5461 cgacatcaat gtaaccttta ccgatacggc gcgtcaggtt tggaacgagg caggcgtggc
     5521 acataaaatc agcctgcatc tgcaacccgc attgctgaca ttggatgatt tgattgcaca
     5581 gggtgaagcc ggaagctacg atttggcact gatagacgca gacaaaccgc ccacgccgca
     5641 atatttcgag cgttgcctca agcttgtccg gcaaggcggc atcattgcca tcgacaatat
     5701 tttgttgaac ggaagggtga tgcgcgaagc ggcttccgat gcgccgccca gcgtcggcat
     5761 cctcaaagat tttaatcaaa acctgccgaa cgacccgcgc atcgtcccca tcaccctgcc
     5821 cgtcggcgac ggtttgaccc tgcttctgaa aaaataatga agaccaaatt accgcttttt
     5881 atcatttggc tgtctgtgtc cgccgcctgt tcttcccctg tttcccgcaa tattcaggat
     5941 atgcggcccg aaccgcaggc agaggcaggt agttcggacg ctattcccta tcccgttccc
     6001 actctgcaag accgtttgga ctatctggaa ggcaaaatcg tccggctgtc gaacgaagtg
     6061 gaaaccttaa acggcaaagt caaagcactg gaacacgcaa aaacacaccc ttccggcagg
     6121 acatacgtcc aaaaactcga tgaccgcaag ttgaaagagc attacctcaa taccgaaggc
     6181 ggcagcgcat ccgcacatac cgttgaaacc gcacaaaacc tctacaatca ggcgctcaaa
     6241 cactataaaa gcggcaggtt ttctgccgct gccgccctgt tgaaaggcgc ggacggaggc
     6301 gacggcggca gcatcgcgca acgcagtatg tacctgttgc tgcaaagcag ggcgcgtatg
     6361 gggaactgcg aatccgtcat cgaaatcgga gggcgttacg ccaaccgttt caaagacagc
     6421 ccaaccgcgc ccgaagccat attcaaaatc ggcgaatgcc aatacaggtt gcagcagaaa
     6481 gacattgcaa gggcgacttg gcgcagtctg atacaggctt acccgggcag cccggcggca
     6541 aaacgcgccg cctcagccat acgcaaacga tagtttttcc atttttccgc tttttacccc
     6601 atccgcacca aggaggattt atgattcaaa tcggcatcat catgggcagc aacagcgatt
     6661 ggcccgttat gcggcaggca gcgcagtttc ttgaagagtt cggcgtagaa tatgaggcgc
     6721 gcgtcgtttc cgcacaccgc accccggatt taatgttcca atacgctgaa accgcacggg
     6781 agcgcggcat caaagccatt atcgcgggcg cgggcggcgc ggcgcacctg cccggcatgg
     6841 ttgccgccaa gaccaccgtc cccgttttgg gcgtacccgt ccccagcaaa tacctgcgcg
     6901 gcgaagattc gcttttatcg attgtacaaa tgcccaaagg cgtacccgtc gccacattcg
     6961 ccatcggcga ggcaggcgcg gcaaatgccg cattgttcgc cgtttccatg ctcgccaacg
     7021 aaacccccga actggcgcaa aaactggcag acttccgagc caaacaggaa caggctgttt
     7081 tgagtatgga attggaacag atttaaaacc cgccgtccgc gaatatatag tggcttaaat
     7141 ttaaaccagt acggcgttac ctcgccttgc cgtactattt gtactgtctg cggcttcgtc
     7201 gccttgtcct gatttttgtt aatccactat acaaatgccg tctgaagccc tgtttcgggt
     7261 ttcagacggc atttttgcga acaacttttt atgcctgttc tttttggcgc attttttctg
     7321 ccatcagttc gtgcagggtt ttcttcgccg gtttcatcgg cacgcggttt tgcgtccaac
     7381 ccaactgttt gcgcggggtc aggttgcgga acttggtggc tgcccaaccg aaggcgcggt
     7441 aggttttgct gccgctgaaa ataccgttga atgtgcgcca cgccatttgt tcgccgaagg
     7501 tatgcgatgc gccttgcccc cggatggggt gcggcacggt ttcggtcggc gaacgttgcg
     7561 cttcaacgcg caaacgctgc atttgttcgg taatcgggat gcgtaccgga caaacttcca
     7621 cacacgcacc gcacatggta caggcggttg gcagatcgcg ggtggcatcc agacccaaca
     7681 ggtgagggga aataatctcg ccaatcgggc cgggataggt tgtgccgtat gccgcgccgc
     7741 cgatgcgggt ataaaccggg caatggttca tacacgcgcc gcaacggata cattgcaggg
     7801 tgcggcgcat ttggtcttcg gcataagcct ggctgcggcc gttgtcgagc agaaccaagt
     7861 gcatttcttg cggaccgtct aattcttcac tgcggcgcgg gccggtaatc atattgaaat
     7921 aggttgtgat gttttgaccg atggcggagc gcggcagcag actgtacaag ggtgggatgt
     7981 cggacaattt cgccaccact ttttcaatgc cggtaatagc gatatgcacg ggcggaacgg
     8041 tggtactcaa gcgaccgttg ccctcgtttt ccaccagaca cagcgtacct gtttcagcaa
     8101 cggcaaagtt tacgccactc aagccgacat cggcagtgct gtaaatatcg cgcagggctt
     8161 tgcgggcgaa gccggtcagt tggtccacat cgtcggtcag cggtgtgccg aggttttggt
     8221 ggaacagttc gctcacctgt tctttggttt tatggattgc aggcatcacg atatgggtcg
     8281 gtttttcgcc tgccatttgg acgatgaact cgcccaagtc gctttctacc gctttaatgc
     8341 cttttgcttc aagataatgg ttcagctcga tttcctcgct gaccatggat ttgcctttga
     8401 ccatcagctt gccgtttttg gctgtgatga tgtcgtggat aatttggcag gcttcggtcg
     8461 gggtttctgc ccagtgcact ttcacgccca acttagtcag gttttcttcc agctgctcca
     8521 gcagggctgg caatttagac aatgaacgct gacggacgtg ttcgcacaaa tcgcgcaagc
     8581 tttgcagctc ttcttcgtcg gtcaaaacgg ctttgcgttt ggtcatcagc atatccatcg
     8641 cggtacgcag gcttttgcgc aaaggcttgt cttgaaggga aattgcggcg ttttgcttga
     8701 aagtttccgg cttcatgtgg aacttgatgg tttgcgtagt catgcgtttt cctccaaatc
     8761 ggcaggggaa atgtggtcgg gcaggatggc gaggatgacc aaatcgcgcg ggccgtgcgc
     8821 gccgtaagca agcgtcagtt ggatgtctgc ggttttggac gggccggaaa tcaggaatac
     8881 attggtcggc ataccgtttt ccaccagttt ttcgccttcg acggcgttgt gaaactcgtt
     8941 gtacatcttg gacgtatcga acaggcagaa atgcacgggc ggaacgagac tcaaagtacg
     9001 cggttcttcg gggctggaaa acagcatcag cgtgccggtg cgggcgatgc cgcattgtgc
     9061 gccgctgaag cccgcattga tgttcgtgaa aaactcggtt ttccaagtat cgatttcgcg
     9121 ctcgaaggca atcggttcga tattgctgtc cgccaatgcg gcacgggcaa tttgtccgtg
     9181 ttcggtcgcc aagggcagca ggatgttttt caaacccttg ccttctgccg cttcgcggaa
     9241 aacctgcatc caattgcttt tcgtcaccca ataaatttcg gttttgaccg cgcgcattgc
     9301 ggcagcccaa tgtttcagac gctcggcttc gctgtcccaa gaaacgccct tttcgcggta
     9361 ataatcgaaa accgcaggct cttccatcgg caaggcatcg gcttttttca gttttgccaa
     9421 aatattttca cgcgcgctca tgctttgcct ccggtgcgtt ccaacaagaa ggatgcgata
     9481 tgtttcggac gcggcatatc cggctcgtcc ttggcgattt tgccgccgat gttcatcata
     9541 cagccgcagt ccgcgctgat gatttcggtt gcgccggttt ctttcagcgc ggcgactttg
     9601 tcggttacca tcgcgccgga aatatcggct tgtttgacgg agaatgtgcc gccgaagccg
     9661 caacattcgc tttcgtggtc gtggacgatg cgttcgacgt tttccatacc gtcaatcagt
     9721 tgccagcctg aaagatggac attcatttcg cggcgggcgg cgcaggaagt gtgaacggcg
     9781 actttgagcg gttcgccctt gtcttcgggt ttgaaaccga tggcaagcag gaaatgggta
     9841 aactcgatga tgcggccggc gcaatccaca gccctttcct cgtactcgct gcctttaaac
     9901 agcgtcggcc aatggtgctt catcataccg ccgcacgaac cggacggtac gacaatcggc
     9961 cactcctcgg ggaaaaggtc gagttgcgct ctggcgacat caaaggcttc ggtcggatgg
    10021 ccggatgaat aggcgggctg gccgcagcag ctttgcgcca tcgggaaatg gacgcgtatg
    10081 ccctgttgct cgattagggt aatggcatcc atgcctgctt cgggcataaa aagatcaagg
    10141 acgcaagtgc cgaaaaaata gacatcggtc ggattgctgt cgtatcggat gatttttggg
    10201 ggaatggttg cgctcatgtt ttttgataac gggaaacccg tttttttctg tagaaaggta
    10261 agcgtttact ttaagtaatt gactgttgcg ggtcaagtct aattttaaaa aataatccgg
    10321 tttttcttac aaactacccc ataacgctta ctgtacctta atctgatagt tttcgataat
    10381 aattatcatt acaatgcaat accggttcgt ttgcttgtga acattcaaga tgccgactct
    10441 gacggcattc agacagcgtc tgaaaacaat aacggcacaa ggatggggta caatcgccct
    10501 atggaaaaaa acaccctatc cgcccgcgca ccatcccctt ggctgccgct tctgctggca
    10561 attgccattt ttatgcagat gttggatgcg accattttaa ataccgcact gcctgaaatt
    10621 gccaccgacc tggacgaatc gcctctggat atgcaactgg cagtcgttgc ctatacgctg
    10681 acggttgccc tgctgattcc tttgagcggt tatttggcgg acaggttcgg aacgaaaaaa
    10741 gtctttttcg gttcgattgc cgtttttatg ctcggatcgg cattgtgcgc cgcatcgggt
    10801 tcgctgtttg aattgacgct ttcccgtgtc gttcagggca tcggcggttc gatgctggtt
    10861 ccgataccgc gtctgaccat cttgcgtgtg tacgacaagt ccaagctgct caacgccatc
    10921 aattatgcgg ttatgcccgc attaatcgga ccggttttag ggcctttggc gggcggttat
    10981 ctggtcgaat acgcttcgtg gcactggatt ttcctgctca acctgcccat cggtcttctg
    11041 ggtttcatat tgggacgcaa catcatgccc gatgtcaaag gcaacgatac cgctctggat
    11101 ttcaaaggct atctgatttt ttctgccgcc gcgtgcctct tgttactttc ggcagaaagc
    11161 ctgtcgcacg cgctgcctcc gtattttgca ctgttgccgc tgtgcggcgg actgctgttt
    11221 gcacgccgtt atttccgaca tatgaaaacc gcgtccaaac cgatttattc cgccgacctg
    11281 tttctgatac gcactttccg tctgggactg gcgggcaatc tgttcagccg tctcggcatc
    11341 agctcgattc cttttctgat gcccctgatg tttcaaatcg ctttcggctt cggcgcaagc
    11401 ctgtcgggtt ggctggtcgc ccccgtcgcc ctgtcttcgc tgctggtcaa accgctgatt
    11461 gcaccgctca tgaaacgttt cggctaccgt acggtactgc tttggaacac caagctgctt
    11521 gccgccttca tcatgctgct cgccctgcct gacggaaact cgccgctgtg gatttgggtt
    11581 ttcctctcgc tggcgatcgg cgcgtgcaac tccctacagt tttctgccat gaacacactg
    11641 accctcgccg atttgcgccc gcaacagacc ggcagcggca acagcctgat ggcggtcaac
    11701 caacagcttg ccatcagcat gggcattgtt gtcggcgcat taatccttaa aaactggaca
    11761 tttctgatac cggcttcttc aggtctgcat tccgccttcc gtatgaccct gctcagtatc
    11821 ggcggcatca cccttgcctc gtcgttggtt ttcaagcgtc tgcacgtttc agacggcgca
    11881 aacctgacac ggaacacact gtcctaaagc ggtccgcacg caaaactttt acccgtttca
    11941 acgtttggat tatgattccg cacttccatg cgcgccaacc ccaaaacaca ggcaatgccg
    12001 tctgaaacca tatccctgat gaaaacacgc agcctaattt cccttttatg cctccttctc
    12061 tgttcatgtt cttcatggtt gcccccactg gaagaacgga cggaaagccg tcatttcaat
    12121 acttccaaac ccgtccgcct ggacaacatc ctgcaaatcc ggcacacccc tcataccaac
    12181 gggctatccg atatctatct gttgaacgac ccccacgaag cctttgccgc ccgcgccgcc
    12241 cttatcgaat ctgccgaaca cagcctcgat ttgcaatact acatctggcg caacgacatt
    12301 tccggccgac tgctgttcaa cctcgtgtac cttgccgcag aacgcggtgt gcgcgtacgc
    12361 ctgctgttgg acgacaacaa cacgcgcgga ttggacgacc tcctgctcgc cctcgacagc
    12421 catcccaata tcgaagtgcg cctgttcaac cccttcgtct tacgaaaatg gcgcgcactc
    12481 ggctacctga ccgacttccc ccgcctcaac cgccgcatgc acaacaaatc ctttaccgcc
    12541 gacaaccgcg ccaccatact cggcggacgc aatatcggcg acgaatactt caaagtcggt
    12601 gaggacaccg ttttcgccga cctggacatc ctcgccaccg gcagcgtcgt tagcgaagta
    12661 tcgcacgact ttgaccgcta ctgggcaagc cattccgccc acaacgccac gcgcatcatc
    12721 cgcagcggca acatcggcaa gggtcttcaa gcactcggat acaacgacga aacgtccaga
    12781 cacgcgctcc tgcgctaccg cgaaaccgtc gaacagtcgc ccctctacca aaaaatacag
    12841 gcaggacgca tcgactggca gagcgtccaa acccgcctca tcagcgacga ccctgcaaaa
    12901 ggactcgacc gcgaccgccg caaaccgccg attgccgggc ggctgcaaga cgcgctcaaa
    12961 cagcccgaaa aaagcgtcta tctggtttca ccctatttcg tccccacaaa atccggcaca
    13021 gacgcactgg caaaactggt gcaggacggc atagacgtta ccgttctgac caactcgcta
    13081 caggcgaccg acgttgccgc cgtccattct ggctatgtca aataccgaaa accgttgctc
    13141 aaagccggca tcaaactcta cgagctgcaa cccaaccatg ccgtccccgc cacaaaagac
    13201 aaaggcctga ccggcagctc cgtaaccagc ctgcacgcca aaaccttcat tgtggacggc
    13261 aaacgcatct tcatcggttc gttcaacctc gacccccgtt ccgcgcgtct caacaccgaa
    13321 atgggcgttg ttatcgaaag ccccaaaatc gcagaacaga tggagcgcac ccttgccgat
    13381 accacccccg aatacgccta ccgcgttacc ctcgacaggc acaaccgcct gcaatggcac
    13441 gatcccgcca cccgaaaaac ctacccgaac gaacccgaag ccaaactttg gaaacgcatc
    13501 gccgcaaaaa tcctatccct gctgcccata gaaagtttat tatagaaata tagcg
//
LOCUS       DAXHSJ010000041        13541 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000041 DAXHSJ010000000
VERSION     DAXHSJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.041"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(148..1191)
                     /gene="argC"
                     /locus_tag="ACFM8U_000862"
     CDS             complement(148..1191)
                     /gene="argC"
                     /locus_tag="ACFM8U_000862"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC6334128.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDALRLLEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEASDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETVLRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLDAIPLLP"
     gene            complement(1188..1469)
                     /locus_tag="ACFM8U_000863"
     CDS             complement(1188..1469)
                     /locus_tag="ACFM8U_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334129.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            1831..2979
                     /locus_tag="ACFM8U_000864"
                     /pseudo
     CDS             1831..2979
                     /locus_tag="ACFM8U_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            2988..3215
                     /locus_tag="ACFM8U_000865"
     CDS             2988..3215
                     /locus_tag="ACFM8U_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334130.1"
                     /translation="MKNWKQFTFFVILVIACYQVLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            3208..3561
                     /locus_tag="ACFM8U_000866"
     CDS             3208..3561
                     /locus_tag="ACFM8U_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334131.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRDWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3961..4400
                     /locus_tag="ACFM8U_000867"
                     /pseudo
     CDS             3961..4400
                     /locus_tag="ACFM8U_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4729..5031
                     /locus_tag="ACFM8U_000868"
     CDS             4729..5031
                     /locus_tag="ACFM8U_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334132.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            5315..5467
                     /locus_tag="ACFM8U_000869"
                     /pseudo
     CDS             5315..5467
                     /locus_tag="ACFM8U_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
     gene            5732..7012
                     /locus_tag="ACFM8U_000870"
                     /pseudo
     CDS             5732..7012
                     /locus_tag="ACFM8U_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222098.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            7121..13066
                     /locus_tag="ACFM8U_000871"
     CDS             7121..13066
                     /locus_tag="ACFM8U_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6334133.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTEILARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNDGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARVDAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSAPGGYIVDIPKGNLKTEIEK
                     LSKQPEYAYLKQLQVAKNINWNQVQLAYDRWDYKQEGLTEAGAAIIALAVTVVTSGAG
                     TGAVLGLNGAAAAATDAAFASLASQASVSFINNKGDVGKTLKELGRSSTVKNLVVAAA
                     TAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILAA
                     LVNTAHGEAASKIKQLDQHYIVHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIVGEA
                     LTNGKNPDTLTAKEREQILAYSKLVAGTVSGVVGGDVNAAANAAEVAVKNNQLSDKEG
                     REFDNEMTACAKQNNPQLCRKNTVKKYQNVADKRLAASIAICTDISRSTECRTIRKQH
                     LIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQADLALQSYHLNTAAKSWLQSGNTKPL
                     SEWMSDQGYTLISGVNPRFIPIPRGFVKQNTPITNVKYPEGISFDTNLKRHLANADGF
                     SQKQGIKGAHNRTNFMAELNSRGGRVKSETQTDIEGITRIKYEIPTLDRTGKPDGGFK
                     EISSIKTVYNPKKFSDDKILQMAQNAASQGYSKASKIAQNERTKSISERKNVIQFSET
                     FDGIKFRSYFDVNTGRITNIHPE"
     gene            13082..13513
                     /locus_tag="ACFM8U_000872"
     CDS             13082..13513
                     /locus_tag="ACFM8U_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334134.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTSCSLHET"
BASE COUNT         4000 a         3074 c         3168 g         3299 t
ORIGIN      
        1 attcccgctt ttgcgggaat gacggtattt tagatttttg ttttcgcgga ataatgagcc
       61 aaaagccgtc cgaacccaaa aaaaccgccg ctttcagatg gcatcaaaaa accgcaggca
      121 cgatgcctgc ggtttgccgt ttgcgtttca ggggagcagg gggattgcgt ccaagccgtg
      181 tgtttccttc agtccgaaca tgatgttcat attttgcacc gcctgtcccg ccgcgccttt
      241 gacgaggttg tcgatgacgg aaaggacgac ccacacatcg gattgcgccg cctgttggat
      301 gctgatgcgg cagaggtttg cgccgcgcac gctgcgggtt tcgggtgtcg aaccggcggg
      361 caggatgtcc acgaacaggc tgtcgcgata atagtcgcgc aaaacggttt cggggcagat
      421 gccgtctgaa aggtggaggt aaacggtggc gtgcataccg cgtatcatcg gcgcgaggtg
      481 cggcgtgaac acgaatcctt cggcgatgcc gtcctgaagc ccggcgatgg tctgcctgat
      541 ttcgggcagg tggcggtgtc cggctatgcc gtaggctttg aagttgtcgc tggcttcgca
      601 caacagcgaa ccgacattgc ctttcctgcc cgcgccggat acgccggatt tgcagtcggc
      661 aatcagcggc ataccgggct tcagacggca ttgccgcaac agcggcacga gcggtaggga
      721 tacgcaggtc gggtagcagc cggggttggc gacgaggcgc gcctgtgcga cggcttcgcg
      781 gttgagttcg ctcaatccgt acacggcttg ggaaacgagg ccgggggcgg cgtgggtcat
      841 gccgtaccag tgttcccagg tcggaatgtc ccgtatgcgg aagtcggcgg aaaggtcgat
      901 gacgcgcacg ccctgttcca aaaggcgcag cgcgtctttc atagcgatgc cgttgggcgt
      961 ggcgaagaag acgatgtcgc attgttccaa acctgcctcg tcgggcgttt ggaaggcgag
     1021 gccgtacacg ccgcgcagac ttggaaagta atcggcaact gcggttcccg cttcgctgcg
     1081 gctggttacg gcggcgactt cgacatcggg atgggcggca agcaggcgca gcagttccac
     1141 gccggtgtag cccgtcgcgc cgacaatgcc gactttgatt tttttactca tggtttttcc
     1201 tttgtgtggt tggcgggtat gccgtccgaa cgctgtccga cggggttttg attggcggcg
     1261 gaaggacggt acacaaagcg tttgaaaagc gtgtctaatt taatggcatc cgcctctgtt
     1321 tttaaaaccc agccctgccg tccggaataa acataaccgt aggcgcgaaa gtcggcagga
     1381 tatttcggtg cctgccccaa acccaaaccg tatgcggcga aagcggtggt cgtggagaag
     1441 gaaagcagga ggaggacgag ggttttcata aggttccaaa ccgaacgggg cgatgaagcc
     1501 cgattatagc aaaaggtcgt ctgaaaaccg ttttcagacg accttttatt taattacaat
     1561 aaaaatgtta acaacaaaaa acaaaccgct ttttttcgtt tggaaacaaa acaaattttt
     1621 atagtggatt aaatttaaac cagtacagcg ttgcctcgcc ttgccgtact atttgtactg
     1681 tctgcggttt cgtcgccttt tcctgatttt tgttaatcca ctataacaaa attggcaaca
     1741 attccttgac gcacacacaa aaaaaacttt aatatttgta aatatatgta acattataaa
     1801 ttttaaatat tttctgagta aggggaagta atgagcatcc atctcgactt cggcattagt
     1861 cctaaaacgt tccgacagac ttatctgtat caaaagccca agctctttaa aggagcggtt
     1921 cggaatctcg aagccgcatc ttggaaagat atcaacgata tataccaacg agcagaccca
     1981 accgcaccgc tgtttcatct gcgtaaaaaa ggtgcaattg ttcctaaaga agaatacgtc
     2041 gaaagtttcg acgatttggg caaaactcgc taccgtttta ttaaatccgt tatctacgaa
     2101 catatgaaga atggcgcgtc gttgctctat aaccatatta tagtggatta aatttaaacc
     2161 agtacagcgt tgcctcgcct tgccggcgca cataccattg ttagtgggta tcttgctttt
     2221 ggcagcgacg aatcttataa aaaccattgg gacatccgag atgtgtatgc catccagctt
     2281 ttcggcaaga aacgttggca acttactgcc cctgatttcc ctatgccatt gtatatgcaa
     2341 cagactaaag atactgatat ttccattcct gaacatatcg atatggatat tatccttgaa
     2401 gcaggtgatg tcctctacat cccgcgcggt tggtggcaca gacctatccc gctcggctgt
     2461 gaaaccttcc acctcgctgt cggtaccttt ccgcccaacg gctataatta cctcgagtgg
     2521 ctaatgaaga aattccccac gatagaaagt ctgcgccaca gtttctcaga ctgggagcaa
     2581 gataggacgc gtatcaacga tactgccgca caaattgctg ccatgattgc cgaccccgtc
     2641 aattatgaag ccttcagtga agactttctc ggcaaagaac gtaccgatac cgcttttcat
     2701 ctcgaacagt tcgcgaatcc caacgctact ccgctttcag acgacgtcag gttgagatta
     2761 aatgccaata atttggatac gttggaaaag ggatatttga ttgggaatgg gatgaagata
     2821 agcgtagatg agttggggaa aaaagtttta gaacacatcg gtaagaatga accgttattg
     2881 ttgaaaaatc tactggttaa cttcaatcag ggaaaatatg aagaagttag gaagttgatt
     2941 tatcagttga tagagttaga ttttctggaa attttgtgag ggattctatg aaaaactgga
     3001 agcaatttac atttttcgta atattagtaa tagcttgtta tcaagtgcta tattttttat
     3061 cagatatgtt tcttctcgac tacattaaca aatatagttg gaatttgaat tttattcagg
     3121 gtactttgaa ttttttttca atatatctgc catatgtttt tgttagccga atttttagga
     3181 ataccaacca agaaaaggag tataaaaatg attgaacttc aacttcatga attgaagctg
     3241 gtttcagggg gaggtcctgt aacagacaat atagctggaa atgtagctaa tgctgccaca
     3301 accaaaggag gtcccacatg gggggatttg gttgcaatac ctgctgccgc agcggcagtt
     3361 tattatctgc cgaaaaatgc ttacggtgct gctggtgcaa atggtgtata caatgtgact
     3421 cgtgattggg taaatgatgc cgttaatgca cctccttata acggaagacc aatctttgag
     3481 attgaacatg gattaactgc tcccgcaaca aaagcagata aatcaggaaa cggctacact
     3541 gacggtacag attactgctg atatttctca ttgttcagac gatctctagg ggtcgtctga
     3601 aactttctta acttcaattt tatgaataga cccaagcaac ccttcttccg tcccgaagtc
     3661 gccgttgccc gccaaaccag cctgacgggt aaagtgattc tgacacgacc gttgtcattt
     3721 tccctatgga cgacatttgc atcgatatct gcgttattga ttatcctgtt tttgatattt
     3781 ggtaactata cgcgaaagac aacagcggag ggacaaattt tacctgcatc aggcgtaatc
     3841 agggtgtatg caccggatac ggggacaatt acagcgaaat tcgtggaaga tggagaaaag
     3901 gttaaggctg gcgacaagct atttgcgctt tcgacctcac gtttcggcgc aggaggtagc
     3961 gtgcagcagc agttgaaaac ggaggcagtt ttgaagaaaa cgttggcaga acaggaactg
     4021 ggtcgtctga agctgataca cgggaatgaa acgcgcagcc ttaaagcaac tgtcgaacgt
     4081 ttggaaaacc agaaactcca tatttcgcaa cagatagacg gtcagaaaag gcgcattaga
     4141 cttgcggaag atcagtagca aaaacggcac tgggcagaca ggaattgtcg ggattgggca
     4201 tggtatcctc cgatttggcg aagagcaacg aacctgttta tcttgtgaaa ataaaacccg
     4261 acaaaccaac catcactgca tacggtgagg aaaaaccgct gcaaatcggc atgacgttgg
     4321 aagcagacat cctgcacgag aaacggcgat tgtatgaatg ggtattggag ccgatttata
     4381 gtatatcggg caaactgtaa gacttgaggc tgaaagataa agaagttgtc tgaaaccttt
     4441 tcagacgacc agaagacaat atataaaaac acaaacttca aaactgcctg aaaccatccc
     4501 cttttacagg cactccgtct acaggcactc cgtctgtaaa agcaaggata gggcatctgc
     4561 ccttttgatt gtttgattaa cgatacaagg agtttcaaaa tgagagtttt atagtggatt
     4621 aaatttaaac cagtacagcg ttgcctcgcc ttgccgtacc atttgtactg tctgcggctt
     4681 cgtcgccttg tcctgattta aatttaatcc actatatgtg ttcatgaaat gacttgggtc
     4741 ggaggctcag gtaatgcgca acaaagttca tattattgcg aaatttgcga atctgcaggg
     4801 cttaacgata cgggaaatcc tgataaatct ttaggattgc caaacaatac gttcagtaat
     4861 ccgcctggtt ggggagctac aatcggagct ttagcaggta gccgcatagg gatgcctgaa
     4921 tttggtacgt ttgcgagcca tgccattgaa aatttcgact ggtcatggta tcgacgttat
     4981 agggaaattg ccgaaacgat tgaacgagaa tattcaggcg gtttgcctta atagttgagg
     5041 aggtcatgat gtttgccaaa cattatcaat tcatcgcact cggcatcatg ctgcttcttt
     5101 atatgttgat tctctatacg accgattttt ccaatctgac gtattggatg ctgtttttta
     5161 tctgttttat tacaggaaaa atattagctc gtttgttaga gaaaagcttt aaataaaata
     5221 gcagctagtc gcaaaaggtc gtctgaaacc ttttcaggcg gcctttctaa aatacatcca
     5281 acttcctaat ccctattttt caaaaaggaa atctatgccc catctgcaaa acctgtcttt
     5341 gggcttaaag aaaaagctgc ctgttatcct gcaaacagaa atatcagaat gcggcttggc
     5401 atgtctggcg gctgtggcgg gatttcatgg tttccatacg aatttacgcg cactgcgttc
     5461 aaaatactgt ccgagacctt tgcaaaattc cccaaaatcc cctaaatgtc ttggtgggaa
     5521 ttttggggaa ttttgcaaag gtctcattct ataactgtaa atacttttaa atttatgaca
     5581 aaatagtaaa tattgctaaa ataatattga tgtcatgaaa ttttttcctg ctccatgtct
     5641 gttggttatc ctggctgtca taccccttaa aaccttagct gccgatgaaa acgatgcaga
     5701 acttatccgt tccatgcagc gttagcagca catagatgct gaattgttaa ctgatgcaaa
     5761 tgtccgtttc gagcaaccat tggagaagaa caattatgtc ctgagtgaag atgaaacacc
     5821 gtgtactcgg gtaaattaca ttagtttaga tgataagacg gcgcgcaaat tttcttttct
     5881 tccttctgtg ctcatgaaag aaacagcttt taaaactggg atgtgtttag gttccaataa
     5941 tttgagcagg ctacaaaaag ccgcgcaaca gatactgatt gtgcgtggct acctcacttc
     6001 ccaagctatt atccaaccac agaatatgga ttcgggaatt ctgaaattac gggtatcagc
     6061 aggcgaaata ggggatatcc gctatgaaga aaaacgggat gggaagtctg ccgagggcag
     6121 tattagtgca ttcaataaca aatttccctt atataggaac aaaattctca atcttcgcga
     6181 tgtagagcag ggcttggaaa acctgcgtcg tttgccgagt gttaaaacag atattcagat
     6241 tataccgtcc gaagaagaag gcaaaagcga tttacagatc aaatggcagc agaataaacc
     6301 catacggttc agtatcggta tagatgatgc gggcggcaaa acgaccggca aatatcaagg
     6361 aaatgtcgct ttatcgttcg ataacccttt gggcttaagc gatttgtttt atgtttcata
     6421 tggacgcggt ttggtgcaca aaacggactt gactgatgcc accggtacgg aaactgaaag
     6481 cggatccaga agttacagcg tgcattattc ggtgcccgta aaaaaatggc tgttttcttt
     6541 taatcacaat ggacatcgtt accacgaagc aaccgaaggc tattccgtca attacgatta
     6601 caacggcaaa caatagtttt tctacgcaac cgccattcaa gctcaatgga acaaaacgcc
     6661 tttggttgcc caagacaagt tgtctatcgg cagccgctac accgttcgcg gatttgatgg
     6721 ggagcagagt cttttcggag agcgaggttt ctactggcag aatactttaa cttggtattt
     6781 tcatccgaac catcagttct atctcggtgc ggactatggc cgcgtatctg gcgaaagtgc
     6841 acaatatgta tcgggcaagc agctgatggg tgcagtggtc ggcttcagag gagggcataa
     6901 agtaggcggt atgtttgctt atgatctgtt tgccggcaag ccgcttcata aacccaaagg
     6961 ctttcagacg accaacaccg tttacggctt caacttgaat tacagtttct aacctctgaa
     7021 ttttttactg atatttagac ggtctttcct tatcctcaga ctgtcaaact ttacctacgt
     7081 acttggcgcg cagtacgttc atcttcaaaa tggaatagac atgaataaag gtttacatcg
     7141 cattatcttt agtaaaaagc acagcaccat ggttgcagta gccgaaactg ccaacagcca
     7201 gggcaaaggt aaacaggcag gcagttcggt ttctgtttca ctgaaaactt caggcgacct
     7261 ttgcggcaaa ctcaaaacca cccttaaaac cttggtctgc tctttggttt ccctgagtat
     7321 ggtattgcct gcccatgccc aaatcaccac cgacaaatca gcacctaaaa accagcaggt
     7381 cgttatcctt aaaaccaaca ctggtgcccc cttggtgaat atccaaactc cgaatggacg
     7441 cggattgagc cacaaccgct atacccagtt tgatgtcgac gctaaaggtg ctgtactgaa
     7501 caacgatcgc aacaacaacc cgtttctggc caaaggcagt gcgcagttga ttttgaacga
     7561 ggtgcgcggt gcggccagca aactcaatgg catcgttacc gtaggtggtc aaaaggccga
     7621 tgtgattatt gccaacccca acggcattac cgttaatggc ggcggcttta aaaatgtcgg
     7681 tcggggcatc ttaactaccg gtgcgcccca aatcggcaaa gacggtgcac tgacaggatt
     7741 tgatgtgcgt caaggcacat tgaccgtagg agcagcaggt tggaatgata aaggcggagc
     7801 cgactacacc gagatacttg ctcgtgcagt tgctttgcag gggaaattac agggtaaaaa
     7861 cctggcggtt tctaccggtc ctcagaaagt agattacgcc agcggcgaaa tcagtgcagg
     7921 tacggcagcg ggtacgaaac cgactattgc ccttgatact gccgcactgg gcggtatgta
     7981 cgcagacagc atcacactga ttgccaatga aaaaggcgta ggcgtcaaaa atgccggcac
     8041 actcgaagcg gccaagcaat tgattgtgac ttcgtcaggc cgcattgaaa acagcggccg
     8101 catcgccacc actgccgacg gcaccgaagc ttcaccgact tatctctcca tcgaaaccac
     8161 cgaaaaagga gcggcaggca catttatctc caatgatggt cggatcgaga gcaaaggctt
     8221 attggttatt gataccgggg aggacatcgg tttgcgtaac ggagcggtcg ttcaaaacaa
     8281 cggcaaccgc cctgctgcta cggtattaaa tgccggccgt aatttggtta tcgaaagcaa
     8341 agcaaatgtg aacaatgcta aaggttcgac caatctgtcg accggcggcc gtaccgtcat
     8401 caaggaggcc agtattcaga ctggcactac cgtatacagt tccagcaaag gcaacgccga
     8461 attaggcaat aacacacgca ttaccggggc agatgttacc gtattatcca acggcaccat
     8521 cagcagttcc gccgtaatag atgccaaaga caccgcacac atcgaagcag gcaaaccgct
     8581 ttctttggaa gcttcaacag ttacctccga tatccgctta aacggaggca gtatcaaggg
     8641 cggcaagcag cttgctttac tggcagacga taacattact gccaaaacta ccaatctgaa
     8701 tactcccggc aatctgtatg ttcatacagg taaagatctg aatttgaatg ttgataaaga
     8761 tttgtctgcc gccagcatcc atttgaaatc ggataacgct gcccatatta ccggcaccag
     8821 taaaaccctc actgcctcaa aagacatggg tgtggaggca ggctcgctga atgttaccaa
     8881 taccaatctg cgtaccaact cgggtaatct gcacattcag gcagccaaag gcaatattca
     8941 gcttcgcaat accaagctga acgcagccaa ggctctcgaa accaccgcat tgcagggcaa
     9001 tatcgtttca gacggccttc atgctgtttc tgcagacggt catgtatcct tattggccaa
     9061 cggtaatgcc gactttaccg gtcacaatac cctgacagcc aaggccgatg tcaatgcagg
     9121 atcggttggt aaaggccgtc tgaaagcaga caataccaat atcacttcat cttcaggaga
     9181 tattacgttg gttgccggca acggtattca gcttggtgac ggaaaacaac gcaattcaat
     9241 caacggaaaa cacatcagca tcaaaaacaa cggtggtaat gccgacttaa aaaaccttaa
     9301 cgtccatgcc aaaagcgggg cattgaacat tcattccgac cgggcattga gcatagaaaa
     9361 taccaagctg gagtctaccc ataatacgca tcttaatgca caacacgagc gggtaacgct
     9421 caaccaagta gatgcctacg cacaccgtca tctaagcatt accggcagcc agatttggca
     9481 aaacgacaaa ctgccttctg ccaacaagct ggtggctaac ggtgtattgg cactcaatgc
     9541 gcgctattcc caaattgccg acaacaccac gctgagagcg ggtgcaatca accttactgc
     9601 cggtaccgcc ctagtcaagc gcggcaacat caattggagt accgtttcga ccaaaacttt
     9661 ggaagataat gccgaattaa aaccattggc cggacggctg aatattgaag caggtagcgg
     9721 cacattaacc atcgaacctg ccaaccgcat cagtgcgcat accgacctga gcatcaaaac
     9781 aggcggaaaa ttgctgttgt ctgcaaaagg aggaaatgca ggtgcgccta gtgctcaagt
     9841 ttcctcattg gaagcaaaag gcaatatccg tctggttaca ggagaaacag atttaagagg
     9901 ttctaaaatt acagccggta aaaacttggt tgtcgccacc accaaaggca agttgaatat
     9961 cgaagccgta aacaactcat tcagcaatta ttttcctaca caaaaagcgg ctgaactcaa
    10021 ccaaaaatcc aaagaattgg aacagcagat tgcgcagttg aaaaaaagct cgcctaaaag
    10081 caagctgatt ccaaccctgc aagaagaacg cgaccgtctc gctttctata ttcaagccat
    10141 caacaaggaa gttaaaggta aaaaacccaa aggcaaagaa tacctgcaag ccaagctttc
    10201 tgcacaaaat attgacttga tttccgcaca aggcatcgaa atcagcggtt ccgatattac
    10261 cgcttccaaa aaactgaacc ttcacgccgc aggcgtattg ccaaaggcag cagattcaga
    10321 ggcggctgct attctgattg acggcataac cgaccaatat gaaattggca agcccaccta
    10381 caagagtcac tacgacaaag ctgctctgaa caagccttca cgtttgaccg gacgtacggg
    10441 ggtaagtatt catgcagctg cggcactcga tgatgcacgt attattatcg gtgcatccga
    10501 aatcaaagct ccctcaggca gcatagacat caaagcccat agtgatattg tactggaggc
    10561 tggacaaaac gatgcctata ccttcttaaa aaccaaaggt aaaagcggca aaatcatcag
    10621 aaaaaccaag tttaccagca cccgcgacca cctgattatg ccagcccccg tcgagctgac
    10681 cgccaacggt atcacgcttc aggcaggcgg caacatcgaa gctaatacca cccgcttcaa
    10741 tgcccctgca ggtaaagtta ccctggttgc gggtgaagag ctgcaactgc tggcagaaga
    10801 aggcatccac aagcacgagt tggatgtcca aaaaagccgc cgctttatcg gcatcaaggt
    10861 aggtaagagc aattacagta aaaacgaact gaacgaaacc aaattgcctg tccgcgtcgt
    10921 cgcccaaact gcagccaccc gttcaggctg ggataccgtg ctcgaaggta ccgaattcaa
    10981 aaccacgctg gccggtgccg acattcaggc aggtgtaggc gaaaaagccc gtgtcgatgc
    11041 gaaaattatc ctcaaaggca ttgtgaaccg tatccagtcg gaagaaaaat tagaaaccaa
    11101 ctcaaccgta tggcagaaac aggccggacg cggcagcact atcgaaacgc taaaactgcc
    11161 cagcttcgaa agccctactc cgcccaaatt gtccgcaccc ggcggctata tcgtcgacat
    11221 tccgaaaggc aatctgaaaa ccgaaatcga aaagctgtcc aaacagcccg agtatgccta
    11281 tctgaaacag ctccaagtag cgaaaaacat caactggaat caggtgcagc ttgcttacga
    11341 cagatgggac tacaaacagg agggcttaac cgaagcaggt gcggcgatta tcgcactggc
    11401 cgttaccgtg gtcacctcag gcgcaggaac cggagccgta ttgggattaa acggtgcggc
    11461 cgccgccgca accgatgcag cattcgcctc tttggccagc caggcttccg tatcgttcat
    11521 caacaacaaa ggcgatgtcg gcaaaaccct gaaagagctg ggcagaagca gcacggtgaa
    11581 aaatctggtg gttgccgccg ctaccgcagg cgtagccgac aaaatcggcg cttcggcact
    11641 gaacaatgtc agcgataagc agtggatcaa caacctgacc gtcaacctag ccaatgcggg
    11701 cagtgccgca ctgattaata ctgctgtcaa cggcggcagc ctgaaagaca atctggaagc
    11761 gaatatcctt gcggctttgg tcaataccgc gcatggagaa gcagccagta aaatcaaaca
    11821 gttggatcag cactacatag tccacaagat tgcccatgcc atagcgggct gtgcggcagc
    11881 ggcggcgaat aagggcaagt gtcaggatgg tgcgataggt gcggctgtgg gcgagatagt
    11941 cggggaggct ttgacaaacg gcaaaaatcc tgacactttg acagctaaag aacgcgaaca
    12001 gattttggca tacagcaaac tggttgccgg tacggtaagc ggtgtggtcg gcggcgatgt
    12061 aaatgcggcg gcgaatgcgg ctgaggtagc ggtgaaaaat aatcagctta gcgacaaaga
    12121 gggtagagaa tttgataacg aaatgactgc atgcgccaaa cagaataatc ctcaactgtg
    12181 cagaaaaaat actgtaaaaa agtatcaaaa tgttgctgat aaaagacttg ctgcttcgat
    12241 tgcaatatgt acggatatat cccgtagtac tgaatgtaga acaatcagaa aacaacattt
    12301 gatcgatagt agaagccttc attcatcttg ggaagcaggt ctaattggta aagatgatga
    12361 atggtataaa ttattcagca aatcttacac ccaagcagat ttggctttac agtcttatca
    12421 tttgaatact gctgctaaat cttggcttca atcgggcaat acaaagcctt tatccgaatg
    12481 gatgtccgac caaggttata cacttatttc aggagttaat cctagattca ttccaatacc
    12541 aagagggttt gtaaaacaaa atacacctat tactaatgtc aaatacccgg aaggcatcag
    12601 tttcgataca aacctaaaaa gacatctggc aaatgctgat ggttttagtc aaaaacaggg
    12661 cattaaagga gcccataacc gcaccaattt tatggcagaa ctaaattcac gaggaggacg
    12721 cgtaaaatct gaaacccaaa ctgatattga aggcattacc cgaattaaat atgagattcc
    12781 tacactagac aggacaggta aacctgatgg tggatttaag gaaatttcaa gtataaaaac
    12841 tgtttataat cctaaaaaat tttctgatga taaaatactt caaatggctc aaaatgctgc
    12901 ttcacaagga tattcaaaag cctctaaaat tgctcaaaat gaaagaacta aatcaatatc
    12961 ggaaagaaaa aatgtcattc aattctcaga aacctttgac ggaatcaaat ttagatcata
    13021 ttttgatgta aatacaggaa gaattacaaa cattcaccca gaataattta aaggaaaaat
    13081 tatgaaaaat aatatttttc taaacttaaa taaaaaatct ataaataaca accattttgt
    13141 tatttcgatt ttttttgaaa caatttacca atttgaaact aaagatacgc ttttagagtg
    13201 ttttaaaaat attacaacta ccggacattt tggagtaata ggtgctcaat atgaaaaaat
    13261 agatgctacc agatggattg gagattatga agaggtaaat ggatttgagt atattgataa
    13321 agctccttct atttattttt cagttggaga tgatttcaat cctgaagaat taattatacc
    13381 tattaattta gcatatcatt actttaatat tgcaatatct gatttcttaa tagctcaccc
    13441 tgaatatcaa aaaaagtgta aagaaataca aaaaacatat tctcaaacaa gctgtagcct
    13501 gcatgaaacc taaaatccat gcgtaaggtg tgtgctccag c
//
LOCUS       DAXHSJ010000042        13449 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000042 DAXHSJ010000000
VERSION     DAXHSJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.042"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(173..601)
                     /locus_tag="ACFM8U_000873"
     CDS             complement(173..601)
                     /locus_tag="ACFM8U_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC6334135.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(666..2414)
                     /locus_tag="ACFM8U_000874"
     CDS             complement(666..2414)
                     /locus_tag="ACFM8U_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC6334136.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHADSTALLADRHSLPPFGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     IQQTRGCLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTIMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYPVGAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLDNWLFG"
     gene            2741..4174
                     /gene="ccoN"
                     /locus_tag="ACFM8U_000875"
     CDS             2741..4174
                     /gene="ccoN"
                     /locus_tag="ACFM8U_000875"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC6334137.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4201..4812
                     /gene="ccoO"
                     /locus_tag="ACFM8U_000876"
     CDS             4201..4812
                     /gene="ccoO"
                     /locus_tag="ACFM8U_000876"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC6334138.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4817..4987
                     /locus_tag="ACFM8U_000877"
     CDS             4817..4987
                     /locus_tag="ACFM8U_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC6334139.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            5011..5922
                     /gene="ccoP"
                     /locus_tag="ACFM8U_000878"
                     /pseudo
     CDS             5011..5922
                     /gene="ccoP"
                     /locus_tag="ACFM8U_000878"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            5998..6342
                     /locus_tag="ACFM8U_000879"
                     /pseudo
     CDS             5998..6342
                     /locus_tag="ACFM8U_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
     gene            6544..7953
                     /locus_tag="ACFM8U_000880"
     CDS             6544..7953
                     /locus_tag="ACFM8U_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC6334140.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGQEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRAEDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYHTMDEIHSDMMLDSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIQAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPSGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            8054..11263
                     /gene="recC"
                     /locus_tag="ACFM8U_000881"
     CDS             8054..11263
                     /gene="recC"
                     /locus_tag="ACFM8U_000881"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC6334141.1"
                     /translation="MFYLYQSNRLETLAALFAHIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPASLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFQNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYTLECLSDLLESRFEVDKVLALLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAAFLETLTDIVRIWRQPATVDEWVGRCRGLLKTLFQTEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILVEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAEKKEEAGEA"
     gene            complement(11333..12901)
                     /gene="mtrF"
                     /locus_tag="ACFM8U_000882"
     CDS             complement(11333..12901)
                     /gene="mtrF"
                     /locus_tag="ACFM8U_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC6334142.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIYIVSLLNADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13233..13445
                     /locus_tag="ACFM8U_000883"
     CDS             13233..13445
                     /locus_tag="ACFM8U_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334143.1"
                     /translation="MKNEIQKNMDKYNPWHEDDFESYEDIARDVSLTTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3377 a         3246 c         3559 g         3267 t
ORIGIN      
        1 tgcattttga aaatgagttt gagcataaaa ttttagtaac ctatgttatt gcaaaggtct
       61 cccactgtaa aaacggagaa ggacagagga cggcagtaag gtttgccgtc ctctttttca
      121 agttgcggtg gaatgccgct tgaaggtgtg gtttaaacgt ttcggcttgt aatcagacgc
      181 gggcgttgtc cagccgctct ctgaagtttt ccaaatttac gcctgccgct tgggcgcggc
      241 ggatgatttc gtcaaccggc ataccttctg ccgcccggcg gaagccccac aggagggagc
      301 agcgcgtacc ggtccggcaa taggcaagga cgggatattc ggcttgtccg atgagttggc
      361 ggaaggtttc gacatcgtgt ttttggatgt cgcgtgcggt aacgggttgg tgatggaatc
      421 cagtaacgcc tgcttgttcc agccactgtt tgatttgggc gaagtcgggt tgcgattctt
      481 cttcgcggtc ggggcggttg cagatgacgg ttttgatgcc cagttgcgcg atttgttccg
      541 catcggcttt ggtcagttgc ggagaaatat agaggtgttc gtcaagtttc agaatggcca
      601 ttttgtttcc tttactttgc ggttgttttt cagacggcat tttgtgtgca atgccgtctg
      661 aaagtttaac cgaacaacca gttgtccaac aggttccggt ctgtgatatg taggatggca
      721 gtatccgctg ccgttgcctg aaccgtcaag acggtttgac gcaatatgcc ggcacggaag
      781 aagtggatat tgatttttgc accgacaggg tatcgggccc attgtgcggc aaagtcggtg
      841 caggcataac cgtctaaagc aatgattttg tcttgcgggc atagtgccgc agattccgcg
      901 ctgccgccgt tgaagacatg ggtcaggacg atgtggtcgg tgttttgttt gaaacgtgcg
      961 ccaaaatcgc ctgccgacgg gacggggcag atgtgttctg cgtatccgcc gccgtgttgt
     1021 cggggaagcg gcaggaaggt cagtcccacg cctgcggttg ccaggcattc ggcaagcggc
     1081 aaatcttcgg tactgtataa cgctttttgg aaaaaatctt ccaaatccaa gccggtaatt
     1141 tcctgacagc ggatttgcca gtgtttttcc ggaatacccg agtgtgtgtc cctccactcc
     1201 cgatagagtt tgtccattat cgtatcgaga gaatgtctgc cgttgcttcg gttgcgtatt
     1261 atcagatcaa ggcacaatgc ggcaagcgcg cctttctggt agtagctgac gatggcgttg
     1321 gggctgtttt catcaggttt gtaaaatttg ttccacgcgg taaaactcga ttccgccaag
     1381 gtctgcctca aacagccgcg ggtttgttgt atgcgcgtaa tgccttgtgc cagcaggttt
     1441 aaataagatt cgggcgagat ggtgcggctg cgtgccaaaa acaaatcgtc gtaataggat
     1501 gtaatgcctt cgaatgccca tagttgttcg gtatagtttt ctttgtcgag gtcataaggg
     1561 gcgaacgcgg caggtttgat ggatttgacg ttccacgcgt gaaaatattc atgggagaaa
     1621 agtccgagca atgtggtgta ggcatcgtcg gcatcggtca taccgaacgg cggaaggctg
     1681 tggcggtcgg cgagcagggc ggtgctgtcg gcgtgttcca aaccgccgta aatatggtcg
     1741 ccgacgtgga gcaggaacaa atatttttga aacggggcag gggaggaaaa caccgccagt
     1801 tctgtttcgc agattttttt gatatccgaa accagcctgt tgcggtcgaa atcgggatag
     1861 atgccgctta aggcaattgt gtgcggaatg cctgccgcct caaaatctaa aaattcaatc
     1921 aagcccatct cgacaggtcg gtcaatcaat tcggcataag atgccgcctg aaagacaaac
     1981 ctccctgttt ccggcagcgt tgtggcaata cgccattcgg acggaatacc cgtcaattcc
     2041 aagcggtgcg gcagcgtttc cgttccttcg actttcaaaa acaggcacga tccgtcaaaa
     2101 aaaccgcgtt ctgtcgtcag gaaagaacct cgaaccgaca aatcgaatgc atatacggtg
     2161 tagcggattt gccactcgcc gcgtacggcg gcagcgtgcc agcggttttt tgcaatttgt
     2221 tcgagcggca tggacgtgcc gttacaggat gcatggatag aagtgatgtg gcgggaaaaa
     2281 tcccgaatca gatagctgcc cggaacccag tttggcaggc ttatttctat aggaagatca
     2341 tcatcttgtg tgaatgtcag caggatgtgc cactcgtggt cgagagggga cggggttatt
     2401 ttataatgaa tcataactat caaatatatc gttaaacggg atggattaaa tacctttaaa
     2461 atgcagctta acctacatca ataaagtgcc acaaaaaggg aaaattggaa acaaaagcaa
     2521 tggcggtcgg gttgtaattt attataacaa tatgatttat atgtaataaa aaacagatta
     2581 agcaaagggc tcgccggcgg cgcgaatgta attccgcttg gcgtaataag gcagatatgt
     2641 taaatttaga aaaaattacg cttgcccgga ttgcacctta aacaggctgc ggaagcggca
     2701 ggataaaaat ctttattccc ataaattcat ggagactctc atggacacac aaacttacaa
     2761 ctacaaagtg gtgcgccagt tcgccatcat gactgtagtt tgggggattg tgggtatgtt
     2821 ggtcggcgtt atcgtcgccg cccagctttt tgctcctgcc ctcgacttgt ctaatatcgg
     2881 accttggttc cacttcggcc gcctgcgtcc gctgcacacc aatgcggtta ttttcgcatt
     2941 tggcggttgc ggcctgatcg gcacatcata ctacgttgtc caacgtactt gtaatacccg
     3001 tcttttcggc ggttggctgc cggcatttac cttttggggc tggcaggcgg taatcgttgc
     3061 cgccgtcgtc agcttcccta tgggttggac ccaaggtaag gaatatgccg aactggaatg
     3121 gccgatcgat attctgatta ctttggtttg ggtggcttac gccatcgtat tcttcggtac
     3181 gattgccaaa cgtaagatta aacatattta cgttgccaac tggttctacg gcggctttat
     3241 tttggcggtc gcacttttac acatcgtcaa caatatcagc atcccggcag ggttgatgaa
     3301 gtcatacccc gtctattccg gtgcgattga tgctatggtt caatggtggt acgggcataa
     3361 cgccgtgggc ttcttcctga ctgccggctt cttgggtatg atgtactatt tcgtacccaa
     3421 acaagcagcc cgccccgttt actcctaccg cctgtccgtc gttcacttct gggcgttgat
     3481 tttcacctat atgtgggcgg gtccgcacca tcttcactac actgcgctgc ctgactggac
     3541 gcaatctttg ggtatggttc tgtctttgat tctgttcgca ccctcttggg gcggtatgat
     3601 taacggcatc atgaccttgt ccggcgcgtg ggacaaactg cgtacagacc cgattcttaa
     3661 attcctgatt gtatccttgt ccttctacgg tatgtctacc tttgaaggcc cgatgatgtc
     3721 gattaaaacg gtcaatgcat tgagccacta tacggactgg accgtcgcgc acgttcatgc
     3781 gggtgcgttg ggctgggtag gctttgtaac catcggttcc gtctattaca tgattccccg
     3841 tctgttcggc aaagaacaga tgcacagcac caagctggta gaagcacatt tttggattgc
     3901 gaccatcggc gtggttctgt atatcgctgc catgtggatt gccggtgtga tgcagggttt
     3961 gatgtggagt tctttgaacg atgacggtac gctgacctac tcgtttgtcg aatccgtaaa
     4021 acgcaccatg ccttactacg tgatccgttt tgcaggtggt ttattgtatc tgagtggtat
     4081 gtgcattatg gcgtacaacg tgtaccgcac agccatcggt ggtaaagcag tcgatgccga
     4141 aatccctgcg gtttcccaaa cacagcacca ctaaaatact aagaaagata ggctaccaaa
     4201 atgaaattac aacaattggc tgaagaaaaa atcggcgttc tgattgtgtt cacgctgctt
     4261 gtagtcagtg tcggtctgtt gattgaagtt gtgcccttgg cctttaccaa ggcggcaaca
     4321 cagccggcgc cgggcgtgaa gccttacaat gccctgcagg ttgccggacg cgatatttac
     4381 atccgtgagg gctgttacaa ctgccactcg caaatgattc gtccgttccg tgcggaaacc
     4441 gagcgttacg gtcattactc tgttgccgga gagtcggttt acgaccatcc gttccaatgg
     4501 ggttccaaac gtaccggtcc tgatttggca cgtgtgggcg gccgctattc cgacgaatgg
     4561 caccgtatcc acctgctgaa tccccgtgat gtcgtgcctg agtccaatat gccggcattc
     4621 ccgtggcttg cacgcaataa agtcgatgtc gatgcaaccg ttgccaacat gaaggctttg
     4681 cgtaaagtag gtactcctta cagtgatgag gaaattgcga aagcgcctga ggctttggca
     4741 aacaaatccg agctggatgc tgtagtcgcc tatctgcaag gattgggtct ggctttgaaa
     4801 aacgtaaggt aacatcatgg atattaacgg tattcgtgct ctcttcacgg tatggatctt
     4861 tatctgtttc ctgttggtac tctatatcgt cttcaacagg cggaataaga aaaactatga
     4921 taatgccgca aacagcattt ttgatgaaaa ccaagatgcg caagataaga aaagcgaaaa
     4981 ccgttaatat tgtgataacg gagcaaaaca atgaacacaa catcccaatt taccagtaat
     5041 ttctggaata tatatattgc agttattgtc ttactgagct ttatcgcttt ggcttggctg
     5101 ctgctgtctc aaaatgttgt caaacgtccg aagaagggcg aagaagtaca aactacgggt
     5161 catgagtggg acggcattgc cgaatacgac aatccgctgc cccgctggtg gttttggctg
     5221 tgtgttttga cgtggctgtt cggtatcggt tatttggtta tgtatccggg tgtcggcgac
     5281 tacaaaggtc tgctgaaatg gaccagccat aaccaatatg aaaaagaggt caaaaaagcc
     5341 gatgagcaat acggcaaact gtatgccaag tttgcggata tgccgattga aaaagtggca
     5401 aaagaccctc aagccaagca aatcgcccaa aacctgttta acacttattg tatccagtgc
     5461 cacggctctg atgctaaagg ctctaaaggt tttccgaatc tgaccgatag cgattggttg
     5521 tggggcggtg atcccgataa aatccacgag accatcgaaa aaggccgtgt tgcgactatg
     5581 cctgcctggg gccctgcttt gggcgaagaa ggcgtgaaag acgttgccca ttatgtgatg
     5641 tccctttcaa aacccgaagg tcagtatgat gaggaacgtg ccgcgcgcgg acaagccttg
     5701 ttcagcggtc cgcctgccaa ctgtttcact tgccacggcg ataagggaca aggtatccaa
     5761 ggtttgggtc cgaacctgac tgatgacgtg tggttgtggg gcggtacgca aaaatccatt
     5821 atcgaaacca ttaccaacgg tcgcagcagc caaatgcccg cttggggaca tttcttggat
     5881 aaagacaaac tgcatattat gactgcttat gtatggggtc tttccgataa agacggtaaa
     5941 gctccggtga aaaaagccga gcctgcaccg actcccgcac cggcggcaga acccgcagcc
     6001 tctgctcctg cagaagcagc acaagccgcg tccgaagcca aacctgccgc agcagaacct
     6061 aaagccgagg aaaaagctgc acctgctgcc aaagcggacg gcaaataggt ttatgaaacc
     6121 gtttgtgccg cctgccatgg caatgcgatt ccgggtattc cccatgtagg catcaaagcc
     6181 gattgggccg accgcatcaa aaaaggcaag gacacgttgc acaaacacgc gatcgaaggt
     6241 ttcaatacga tgcccgcaaa aggcggtcgc ggcgatttga gcgatgatga ggttaaagct
     6301 gcggttgact atatggtcaa ccagtcaggc ggcaaattct aacttgactg agtttccgat
     6361 attatcggtt atgaaaatcc agattcagta ttgaatctgg attttttgtt ttaacgggca
     6421 tccggtttta aacgcagact gcctatttgt tgtaatgaat tttgacttta gtcataaaga
     6481 attagtcttt attgtttata atcgcgcatc atcacgatag ccctattttc aaaaggacga
     6541 tttatggata cacaaatcaa gactgaagcc gacaatcaga gcaaccggcg ttatctgacc
     6601 gtttggcggt ggcattttta tgccggtctg ctggttatgc cttttcttac cctgcttgcc
     6661 gttacgggtt tgggtatgct gctgtttgcc aatattaccg gtcaggaggg cgagcggatt
     6721 catgttgtgc cgcaggcaac ggtacaacct ctgtctgttc aggcggaagc ggcacgcagt
     6781 gccgttaatc cggaaacttc gtccgtcgtt cagtatattg cgccgcgcgc cgaggacatg
     6841 gttgccgtgt tccgtgtcaa caatgagggt aaggcaacga tggtcgcggt cgatccttat
     6901 acggcaaaag tagtcagcac tatgccgcgc aatcagggtt ggtatcacac gatggatgaa
     6961 atccacagcg atatgatgct cgattcgaca ggcgattatc ttttggaaac ggctgcatcg
     7021 ctgacgatta tcatgataat cagcggtttg tatctttggt gggtgaaacg gcgcggcatc
     7081 caggcgatgc tgctgccgcc aaaaggcagg gcgcgttctt ggtggcggaa tctgcacggc
     7141 gcgtttggaa cttgggtgtc gttgatttta ctgttgttct gcctgtcggg tattgcttgg
     7201 gcaggtattt ggggcggcaa gttcgtgcag gcttggagtc agttcccggc aggcaaatgg
     7261 ggtgtcgaac cgaaccctgt ttcagtcgtg ccgacccacg gcgaggtatt gaatgacggc
     7321 aaggttaagg aagtgccgtg ggttttggag cttacgccta tgcctgtttc agggacgact
     7381 gtgggcaaag acggtattaa ccctgacgag ccgatgacat tggaaaccgt cgaccgtttt
     7441 gcgcgggaaa tcggtttcaa agggcgttat cagctgaatt tgcccaaagg cgaggacggc
     7501 gtatggactt tgtcgcagga ttctatgagt tacgacatga tcagcccgtt tgctgaccgc
     7561 acggtgcata tcgaccagta cagcggcaag attcttgccg acatccgttt tgatgattac
     7621 aacccgttcg gcaaatttat ggcggcgagc attgcgctgc atatggggac tttgggctgg
     7681 tggagcgtgt tggcgaacgt tttgttctgc cttgccgtca tttttatcgg tatcagcggt
     7741 tgcgtgatgt ggtggaaacg ccgtccgtcc ggcgcggtgg gcatcgttcc gccggcgcag
     7801 aaagtcaagc tgccggtttg gtggatgatg gcattgccgc tattggcaat cgcactgctc
     7861 ttcccgaccg cactgcttgc cattgccgtg atttggctgt tggatacctt gctgctgtcg
     7921 cggattcctg ttttgaggag atggtttaaa tgacccgatg ccgtctgaaa ggttcagacg
     7981 gcattttgtt tgaaggcagg cgggggaaag gctatataat cccgaatact tttccgcaac
     8041 ttttgtcgaa atcatgtttt atctgtatca atccaaccgt cttgaaacgc tggcggcact
     8101 gtttgcccac attcaaaaag tcaaaccgct gaaatcggct ttacagcccg aacagattat
     8161 tgtgcagagt caggggatgc gccgctacct caacacctgc ctcgcccgcg atttgggcgt
     8221 ggcggcgaat ttggcgttca gcctgcccgc cagcctgacg tggaagctga tgaaaaaact
     8281 gattcccggc attccggaac tcagcccgtt tgcacccgaa gtcatgcgct ggcggctgct
     8341 ggatttgttc cgcagcgaag cattccagaa tacggcagaa tttgaagatg tgaggaatgt
     8401 gctgcaagac tatctaggca gcggcgaatc ggcagattac cagcttgcgg gacagcttgc
     8461 ggacatattc gaccaatacc ttgtctatcg tccgcagtgg atagacgcgt ggcagcaggg
     8521 caggatactc ggtttgggcg acgatgagat ctggcagtcc aaactgtggc gttacctcga
     8581 cgacggcagg cagagcgcgc cgcaccgtgt cgcgttgtgg gaaaaactgc ttgccgcgtt
     8641 ggacaaggat aagctgcccg agcgttattt cgtgttcggc atttccacga tggcgccgat
     8701 gtatcttcag cttttgcaca agctgtccga acattgcgat gtgttcgtgt tcgcactcaa
     8761 tccgagcggg atgcattggg gcaatgtcat cgaggcggcg caaatcctca aaggtggcgg
     8821 cgatcccgat ttaactcagg cagggcatcc gctgctcgct tcattgggca agcaggggcg
     8881 ggactttttc gactttttga acgaaatgga aatagaaggc gaaacgccgg tatttgaaga
     8941 aggcggacgc gatacgcttt tgcacgccct gcaaaccgat attcaaaacc tgaaaatgcc
     9001 gtctgaaaat gtgggaagcg tcaacacggg cgacggctcg atacgcatcg tctccgcaca
     9061 cagccccttg cgcgaattgc agatactcaa agacaagctg ttgaaaattc tgcatgaaca
     9121 tcccgattgg cagccgcatg atatcgccgt attaaccccg aacatcgaat cctatacgcc
     9181 ttttatcgaa gccgtgttcg gacaggcgca gcccggtgcg caggcattgc cgtattccgt
     9241 ttcagacgtg aaaatcagcc gccgacaacc gtttttttat acattggaat gcctgtccga
     9301 cttgttggaa agccgatttg aagtcgataa agtgctcgcg cttttggaaa ccgcgcccgt
     9361 attgcgccgt ttcggactga ctgaggacga tttgccgctt ttgcacgata tggttgccga
     9421 tttgaacgtt cactggggtt tggacggaga aatgcgcggc ggcacggatc agctgttcac
     9481 ctggaagcag gcggtagaac gcatgatatt gggctggatg ctgcccgaag gcggcaaccc
     9541 gatgtggcag gatgtcagcg cgtggtatgc cgacgtgaac caaaccgccc tgttcggacg
     9601 ttttgccgcc ttcctcgaaa ccctgacgga tattgtacgg atatggcggc agcccgcaac
     9661 ggttgatgaa tgggtaggac gttgccgagg tttgcttaaa acattgttcc aaaccgaagc
     9721 cgatgaccaa aaggcagtcc aaaacctcga aaacgaatgg gtcaaatggc aggcggaaac
     9781 cgaattggcg caattttccg gacagttgcc gccgcacacc gtcatccgcc acatccgccg
     9841 ctttctcgac agcgaaagcg aggcaggctt tttacgcggc ggcatcacct tttgcagtat
     9901 ggtgccgatg cggagcctgc cctttaaagt catctgtctg ttgggtttga acgacggaga
     9961 ttttccccgc aataccaaag ccgccgtatt cgacctgatt gccaaacatc ccgccaaagg
    10021 cgaccgcgcc cgccgcgacg acgaccgcta cctcttcctc gaagccctca tcagcgcgcg
    10081 tgaaatcctg tacctgtcct acatcgggcg cgacatccgc aaagacgaag aactggctcc
    10141 gtcttctttg ttgggcgaac tcatcgatac cgttgccgct atggcgggca tcggtagccg
    10201 ccaacttgca caaaacctga tagaacagca tccgctgcaa gccttctcgc gccgatattt
    10261 tcaagaaggc ggacgttcag acggcatatt cggcacgcgt accgactacg ccgccgcgct
    10321 cggacaaacg cccgaaccgc cgcaaccctt tttcgatcaa cccgtagaaa acgccgaacc
    10381 tgttgccgaa atcggacagg acgaatttat ccgcttctgg cgcaaccctg tcaaagtatg
    10441 gcttcagcag cagcttgcgt ggagcgaacc ccatatcggc gaagcctggg agcctgccga
    10501 acccttcgag ccgcaacacg ccgatcaaat cgccgaaatc tacatcgaag cacggcgcga
    10561 aggacgggat tttgcccaaa ccgccgcccg catcggggcg gaaagcctcc tgccgtcggg
    10621 agagttgggc agactttggc agcaggactt ccaaaccgct gccaaacaaa tcgacacggc
    10681 ggttttaaac agtcccaaac ttcctccgct gccctacgcc ataccgtcgg acgggcaaat
    10741 cctgaaaggc agcttgggca atctgtaccg ctgcggacaa gtgttttacg cctacggcaa
    10801 acccaacgcg ccgcaacgta tcgctttttt gttggaacac ctgatatttt gcgccgttat
    10861 gccgtctgaa gccgaaacgc ggcaaacctt tatcgtccaa tccggagaaa ccgaaatatt
    10921 ggtggaaatc gcgcaagaca gggcattgca gctattgtcg gaatggatgg cgtttttcaa
    10981 tatcgggcaa aaccgcccgc tgccgttttt tgccaaaacc tcgcttgcca ccgccgaagc
    11041 gtttgcccaa aaacaagatt gggaagccgc cctgaaaaaa gcccaaaccg cctaccacgg
    11101 caacaaagtc agcaaagggc agaaagacta taccgaagtc gccctcgtgt tcggcaacgc
    11161 aagccaaaac ccgctcgaac agcccctgtt tgaaaacctc gtccgtctgc ttgccgacac
    11221 gcttgccgca gcggaaaaaa aggaagaggc cggagaagcc tgacgggatg gaaaggcaaa
    11281 taccgtctga aaacagcaaa aaggttttca gacggcattt tgtttatcgt gtttaaggtg
    11341 cgggatagaa tgtgggcgcg ccgggaccga cgggcaggcc caaaacaaat acccaaatgc
    11401 agaataaggc aatccacgca atcaagaaga aagcggaata cggcaacatc atagaaatca
    11461 gcgtacccac gcccgcatct tttttgtatt tgatcaccgt cgccataatc agcccgaaat
    11521 aactcatcat cggcgtaata atattggtaa cggaatcacc gatgcggtaa gcggcttgaa
    11581 tgacttcggg cgcgtagccg gccaacatca gcatagggac gaaaatcggc gcagttaccg
    11641 cccattgcgc ggaggcggag cctatcatca gattgataaa agcacaaatt aaaataaaac
    11701 cgataaacaa cacgctgccg cccaagccga cttcttttaa gaacgtcgcc cctttaacgg
    11761 caatatattg cccaatattc gtccaattaa aaaatgcgac aaactgtgcg gcaaaaaaga
    11821 tgatgaccaa ataaagcccc agagtactca tcgattcggc catcgcatta acgacttcct
    11881 gttcgccgcg caaacttcgg gttacccggc cataaacaat gcccggcagt gcaaacaaca
    11941 agaaaataaa aacaacaatc gattttaaaa acggcgaacc ggaaaccaat cctgtttcag
    12001 gatgacgcaa aataccgtcg gcagggacga tgctccaagc caatagggcg gataaggcaa
    12061 caaacaccac gccagcccaa attaatcctt tatattccaa aggcgtgatt tcattggaat
    12121 gccgaatgtc tttttcttct tgtgacaaat ctgattgata agggcccaat tgcggttcga
    12181 cgattttttc agtaacaaaa taaccaatca aagcaatcac aaacgtactg gctaccataa
    12241 aaaaccagtt ggcttcaggg cctacgacgt agtcgggatg gatgatttgc gccgcctgtt
    12301 gggtgatgcc tgccaagagc ggatcgattg tgcctaagaa cagattggcc gaataaccgc
    12361 ccgaaacgcc ggcgaaagcc gcagccagac cggcaagcgg atggcggccg agggaatgaa
    12421 agatgatggc ggacaaaggg attaggacga catagcccaa ttcagaagcg gtattagata
    12481 aaatccctgt aaaaacaacc ataaaagtag tgagtttgcg tggcgatttt gtgagcaata
    12541 agcgcattaa tgcggaaatc aagcccgatt tttccgcaat ccccacgccc aataaagaaa
    12601 ccaacaccgt tcccaacggc gcgaaaccgg tgaaattttt aacggtatgc gtcaggattt
    12661 tgatcaaacc gtcggcattg agcaggctga caatgtaaat caaaccgtca tcggcacgtc
    12721 ctttcgcacc aacagggcgc ggatcgggga cggatagtcc gaaatacgca ccgacggcag
    12781 aggcaatcag caataacaca atgaaaataa taaaaagcgt aaccggatgc ggcaacatat
    12841 tgcccagcca ttcgactgtg cgtaaaaatc gtccgtcccg ttgcgtatcg gtttgactca
    12901 tctgcttctc cttaaaaaaa tatattgagt gcattaaatt tttgaatgta acatgaagtg
    12961 ttttgtaagc aaactaaaat aatcgcatat gtatataacc gcaatagatt aaaacagaaa
    13021 atgcccacat cgttattctc ataaaggcgg taatccgaaa acttgacgtt aaagccttat
    13081 caggaatgac tggaactcaa aaaacggttt cccgttttcg cggaaatggc gggattttca
    13141 gtttttattt gcaaaaatgc tggagaaaaa tgcagtttac attttgaatg gtattgttat
    13201 taattgtaaa taaatattgg aaggtaaatg atatgaaaaa tgaaattcaa aaaaatatgg
    13261 acaaatataa tccctggcat gaagatgatt ttgagtcgta tgaagacatt gccagagatg
    13321 tatcgctgac gacagataaa acgttcattg aacattattt gttagaagtt tattcagaag
    13381 aaaacggaca ttttgaccaa gagaatgtcc atgcaatgat agaagaaatt aaaaatgcaa
    13441 tttagtgaa
//
LOCUS       DAXHSJ010000043        13293 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000043 DAXHSJ010000000
VERSION     DAXHSJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13293
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.043"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..847)
                     /locus_tag="ACFM8U_000884"
     CDS             complement(<1..847)
                     /locus_tag="ACFM8U_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6334144.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLPGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNRLAQDDTLSELLELINRQLTEKGLKIEKASAAVVDATIIQTAGSKQRQAIE
                     VDEEGQISGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECK
                     HLSPLLEGIAESTTVYADKGYDSAENRQHLKEHQLLDGIMRKARRNRPLSEAQTKRNR
                     YLSKTR"
     gene            complement(1340..1447)
                     /locus_tag="ACFM8U_000885"
     CDS             complement(1340..1447)
                     /locus_tag="ACFM8U_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFC6334145.1"
                     /translation="MPSETLPIIGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            complement(1461..1913)
                     /gene="rplI"
                     /locus_tag="ACFM8U_000886"
     CDS             complement(1461..1913)
                     /gene="rplI"
                     /locus_tag="ACFM8U_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC6334146.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPTGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(1930..2160)
                     /gene="rpsR"
                     /locus_tag="ACFM8U_000887"
     CDS             complement(1930..2160)
                     /gene="rpsR"
                     /locus_tag="ACFM8U_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC6334147.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            complement(2167..2469)
                     /gene="priB"
                     /locus_tag="ACFM8U_000888"
     CDS             complement(2167..2469)
                     /gene="priB"
                     /locus_tag="ACFM8U_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC6334148.1"
                     /translation="MGFNNLVSLAVLIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGDCATVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            complement(2470..2838)
                     /gene="rpsF"
                     /locus_tag="ACFM8U_000889"
     CDS             complement(2470..2838)
                     /gene="rpsF"
                     /locus_tag="ACFM8U_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC6334149.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTESSPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            complement(2994..3944)
                     /gene="trxB"
                     /locus_tag="ACFM8U_000890"
     CDS             complement(2994..3944)
                     /gene="trxB"
                     /locus_tag="ACFM8U_000890"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC6334150.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            4083..6245
                     /locus_tag="ACFM8U_000891"
     CDS             4083..6245
                     /locus_tag="ACFM8U_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6334151.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLDIPTAQNAQTVVGAGIAAEVKGAGLVKAGKAEF
                     AELTLPKFSDGVWEIASVVAVSVNGKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            6301..8187
                     /gene="uvrC"
                     /locus_tag="ACFM8U_000892"
     CDS             6301..8187
                     /gene="uvrC"
                     /locus_tag="ACFM8U_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC6334152.1"
                     /translation="MPSESVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVREAATFLNGK
                     TDELTRTLQHKMQAAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPMPDALKEALESEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            8305..9708
                     /locus_tag="ACFM8U_000893"
     CDS             8305..9708
                     /locus_tag="ACFM8U_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6334153.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGARPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTTAKNNNPHGQYLLAQYCQYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVYRFQQAAQENDPDAHAALADIYLQGKHLERNHKLALHHAEAAAAERHP
                     EGLRILGDICRYSLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPNQYGGIK
                     DSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            9830..10546
                     /gene="trmB"
                     /locus_tag="ACFM8U_000894"
     CDS             9830..10546
                     /gene="trmB"
                     /locus_tag="ACFM8U_000894"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC6334154.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTATDYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIR"
     gene            complement(10727..11014)
                     /locus_tag="ACFM8U_000895"
     CDS             complement(10727..11014)
                     /locus_tag="ACFM8U_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334155.1"
                     /translation="MTYSFRVENINDISDIPENHLEIVRAALPSNRSNALSREPDGAQ
                     AVFVYEIDGEFRAKLFDRIKDTDSYLQATEDNYSNDPRSIEVVYVLEQQQG"
     gene            complement(11143..13242)
                     /gene="uvrB"
                     /locus_tag="ACFM8U_000896"
     CDS             complement(11143..13242)
                     /gene="uvrB"
                     /locus_tag="ACFM8U_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC6334156.1"
                     /translation="MPSERRFRPIPTTRFVRKRITGKIMEVIRYPDSPFKLHQPFPPA
                     GDQPTAIADLLEGLSDGLAYQTLLGVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQ
                     LYAEMREFFPENAVEYFVSYYDYYQPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNL
                     MTRDDVIIVATVSAIYGIGDPTEYQQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDF
                     KRGSFRVRGDVIDVYPAESSENALRISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPS
                     SHYVTPRDTVLRACESIKEELRERIEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCK
                     GIENYSRHFSGKKEGEPPPTLMDYLPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLV
                     DYGFRLPSARDNRPLKFHEFEKVMPQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDP
                     QIIIRPVATQVDDLMSEINDRIQKGERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSD
                     IDTVERVEIIRDLRLGLFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQ
                     TIGRAARNVNGVAILYADKITDSMKAAIDETECRREKQIKFNEEHGIVPQQIKKQVKD
                     IIDGVYHEEDSGKGRRQGKNKVKVGEIHNEEDAIKEIAKLEKAMQQAARDLQFEEASV
                     LRDRIRGIREGLLFGSE"
BASE COUNT         3248 a         3512 c         3401 g         3132 t
ORIGIN      
        1 aacgggtctt cgacaaatac cggttgcgtt tggtttgcgc ttccgacagc ggacggttgc
       61 ggcgggcttt gcgcataatg ccgtctaaca actgatgttc tttcagatgt tgccggtttt
      121 ccgcactgtc atagcctttg tcggcataga cggtcgtact ttcggctatc ccttccagca
      181 aaggcgacag gtgtttgcac tcatgggcat tggcgggggt aatgtgcagt ttctcgatat
      241 agccttctgc atcggtacgg gtatgttgtt tgtaaccgag tttgtagagg ccgtttttct
      301 tgatccaacg ggcatcgctg tccttactcg gtgtggtttg gccgctgatt tgtccttcct
      361 catcgacttc tatagcctga cgctgtttgc tgccggcggt ctgaataatg gtggcgtcaa
      421 cgacggcggc ggatgctttc tctattttta aacctttttc ggtcagttgg cggttaatca
      481 gttccagcaa ttcggacagg gtgtcgtctt gcgccagccg gttgcggtag cggcataagg
      541 tgctgtaatc ggggatgctc agttcgtcaa aacggcaaaa caggttgaaa tcgatgcggg
      601 taatgaggct gtgttcgagt tcgggatcgg agaggctgtg ccattgtccg ggcaggacgg
      661 ctttgaacat ggacaacagg ggataggcgg gacggccacg atggtctcta aggtaacggg
      721 ttctttgacg attcaggtat tgttcgatcg gttgccagtc aatcacttga tccaacttca
      781 atagcgggaa gcggtcgatg tgtttggcaa tcatggcttg tgcggtttgc cggaagaagg
      841 tgctcatgag aaatccccta aatgtcttgg tgggaattta ggggattttg ggggattttg
      901 caaaggtctc aaacggtatt tccgccataa tgaccggcat acaagccgca acctttgcaa
      961 tcatatatat aagttattaa atttttagga tgtattttga caatctgttg atttcaactg
     1021 cttgcaaggg aaaaggccat tatttttcgg tcaatcagga agttatccta ttgaacattt
     1081 tgaaacaaag tatgttatca gcaccatatt gaaacacagc caaattttat gcccgatacc
     1141 attttcaaaa taaataatcc cctgattttc acagatttct caaaatcaga aaagtttttg
     1201 tcaattgaat attttggctg tgttttagta atctgttgat ttcaattatt tgcaagggaa
     1261 aagacaatta ttttttcggt taggaagaga cctatcctat tgagtatatt gaagccaggt
     1321 acgctgtact ggtttaaatt taatccacta taaaaacaac catcccgatt tttgcagaat
     1381 atacggaaaa caaaacaaat accgtctgaa accacattcc gataatcggc agggtttcag
     1441 acggcatctg ataatttcaa ttactcggtt gcggcaacga cggcaacggt aattttagca
     1501 acggcatcag tgtgcaaagc cacttccact tcgtactcgc caacggcttt cagaggaccg
     1561 ttcggcagac gtacatttgc tttcacggct tcgatgccgg cagcaacgat tgcagcagca
     1621 atgtcggcat tggtaacgga accgaacagg cgaccgtcca caccggcttt ttgagcaacg
     1681 gtaacggttt gaccgtccaa tttttcctga cggactcggg catctgccaa aatttcagcc
     1741 tgtttggctt ccaattctgc acggcgtgct tcaaactctt tcatattcgc ttcggtcgca
     1801 cgttttgcct tacccgtagg aatcaaaaag ttgcgggcat agccgttttt aacggttacg
     1861 atgtcgccca agttgcccaa accgccgatt ttttctaaca gaataatttg catgattcaa
     1921 tctccaaaat tatttgtgtt ggtcggtgta aggcagcaga gccaggaagc gtgcgcgttt
     1981 tacggcaaca gccaattggc gttggtagaa tgctttcgtt cctgtgatgc gtgcaggaat
     2041 gattttaccg ttttcagaga taaagtcttt tagcaaatca acttgtttgt aatcgacttc
     2101 ttggattttt tcagccgtga aacggcagaa ttttctacgt ttgaatgatt gacgagccat
     2161 tgtcgtttaa cctttatatt cttgaatatt ttgtatcctg agcatcggca tcagggaacg
     2221 tctgcttttt tgagccaaaa aaccttcgac tgttgcgcag tcgccttgcc gatactgcca
     2281 ctcttccgcc tgcctgccta aaatccgtgc cggaatttcc aattggacaa ggcattgctg
     2341 cccgttttcc tcctgccacg attcgtgctt taaaataata tctaaaacag ggattccggc
     2401 aggcgtatat cgaataggga aaaccttttc aattaacacg gcaagcgaaa caagattatt
     2461 gaatcccaat tattgggcga ccgcttcttc agacgcgccg ctcaacaggt tcttagcctt
     2521 ttcaccaccc aacatagggg atgattcggt aacggcgtgt ttggttttga tggttagatg
     2581 gcgcaatacg gcatcattga agcggaatgc ggtttccagc tcttcaacca cttcgggagt
     2641 ggtttcgatg ttcatcaaaa cgtaatgggc tttatggatt ttgttaatcg ggtaagccag
     2701 ttggcggcga ccccaatctt ccaaacggtg aatcttaccg tttgcttcgg caatcatggt
     2761 tttgtaacgt tcaaccatag caggcacttg ctcgctttga tcaggatgaa cgataaacac
     2821 gatctcgtaa tgacgcatgt tatctcctta tggatggtaa aaacagcctt ctgccatgcg
     2881 aaagcagaag gcaaggttca aagagcaggc attatattgg ggtttgccga cggaatcaag
     2941 gatttggtgc gaaaaatttg cattccgccg aaaatttcgg tttcagacgg cattcaaatg
     3001 ttttggctgc ccagccagcg ttccgcgtcc aaagccgcct gacagccgga agccgcgctg
     3061 gtaatcgcct gacggtaggt atggtctttt acgtcgcccg ccgcccatac gccttcgata
     3121 ttggttgcgc cgacattgtc cgccgtgccg cctttggttt tcaggtaacc ggcttcgtcc
     3181 atttccaact gacctttgaa aatatcggta ttcggcttgt gcccgatggc gataaaaatg
     3241 ccgctgacgg caatttgttg ctcagaaccg tcgttgtttt ttaataatgc gccgtttacg
     3301 ccccgatcgt cgcccagtac ttcttgcagg ttgctttcca gcttgaggat gattttgccc
     3361 tcttccacgc gtttcatcag tttgtcgatc atgatttttt cggcacggaa ctcgctgcgg
     3421 cggtggatca gggtaacggt tttggcgata ttggcaaggt agagtgcctc ttcaactgcc
     3481 gtattgccgc cgccaactac ggcaacatct tggtttttgt agaagaaacc gtcgcaggtg
     3541 gcacaagcgg aaacgccttt tcctgcaaac gcttcctcac tcggcaaacc gaggtatttg
     3601 gcggacgcgc ctgttgcgac aatcagggca tcgcaagtgt actcgcccat atcgcctttg
     3661 agtgtaaacg ggcgtttttg cagatcgacg gcgttgattt ggtcaaaaat gatttccgtt
     3721 ccgaaacgtt cggcgtgggc gagaaaccgc gccatcaatt ccggcccttg cacgccgtcg
     3781 gcatcggcag gccagttgtc cacttcggtg gtggtcatca gttgcccgcc ttgcgcgata
     3841 cctgtaataa tgacggggtt taaattggca cgcgcggcat agacggcggc ggtgtatccg
     3901 gcggggccgg aacccaaaat aatcagtttg cggtgttggg acattgtttt tcctttgctg
     3961 tgtcaagttt tcggattcta ctcgaattat cggcgcgttt gagaaatttc gaccataccg
     4021 gcgctcagac ggcatcccgc agccttaact gccgtctgaa tatcaaagca ggaatcacgc
     4081 ttatgcaaca aaaaatccgt ttccaaatcg aaggcatgac ctgccaggcc tgcgcttcgc
     4141 gcattgaaaa agtgttgaac aaaaaagatt ttgtcgaatc ggcgggggta aacttcgcca
     4201 gcgaagaggc gcaggtagtg tttgacgaca gcaaaacctc agtagccgac attgccaaaa
     4261 tcattgagaa aaccggttac ggcgcgaagg aaaaaacgga agatacattg ccgcaacccg
     4321 aagcagaaca ccatatcggc tggcggctgt ggctgctgtt caccatcaac gtcccgttcc
     4381 ttatcggcat ggcggggatg atgatcggca gacacgattg gatgattccg ccgttgtggc
     4441 agttcgcatt ggcgagtgtg gtgcaacttt ggctggcggt gccattttac aaaagcgcgt
     4501 gggcgagcat taagggcgga ctggcgaata tggacgtgct ggttaccatc ggcacggtct
     4561 cgatttacct gtattccgtc tatatgctgt ttttcagccc gcacgcggcg tacggtatgg
     4621 cgcatgtgta ttttgaagtg ggcgtgatgg tgattggttt tgtgtcactg ggcaaatttt
     4681 tggaacaccg taccaaaaaa tccagcctca acagcttggg cttgctgctc aaacttacac
     4741 caacccaagt caacgtgcaa cgcaacggcg aatggaaaca gcttcccatc gaccaagtgc
     4801 aaatcggcga ccttatccgc gccaaccacg gcgaacgcat tgccgcagac ggcatcattg
     4861 aaagcggcag cggttgggcg gacgagagcc atcttaccgg cgaatccaat cctgaagaaa
     4921 aaaaggcggg cggcaaagtg ttggcgggcg cgttaatgac cgaaggcagt gtggtgtacc
     4981 gcgccacgca gctcggcagc caaacccagc tcggcgacat gatgaacgcg ctctctgaag
     5041 cacaaggcag taaagcaccg attgcgcgcg tggcggacaa ggcggcggcg gtgttcgtgc
     5101 ctgccgtcgt gggcatcgcg ttgttgactt ttattgttac ttggctgatt aagggcgatt
     5161 ggacggttgc gctgatgcac gccgtcgccg ttttggtgat tgcctgcccg tgtgcgctcg
     5221 gtctggctac acccgccgcg attatggtcg gcatgggcaa agcggtgaaa cacggtattt
     5281 ggtttaaaga cgcggcggca atggaagaag ccgcccacgt cgatgccgtc gtgctggaca
     5341 aaaccggcac actgaccgaa ggcaggccgc aggttgccgc cgtttattgc gttcccgaca
     5401 gcggctttga cgaagacgct ttgtaccgca tcgccgccgc cgtcgaacaa aacgccgctc
     5461 atccgctcgc ccgagccatc gtttccgccg cccaagcgcg cggtttggac attcctaccg
     5521 cacaaaacgc ccaaaccgtt gtcggcgcag gcattgccgc cgaagtaaaa ggcgcgggtt
     5581 tggtaaaagc aggcaaagcc gaatttgccg aactgacctt gccgaagttt tcagacggcg
     5641 tttgggaaat cgccagtgtg gttgccgtat ctgtaaacgg caaacctatc ggcgcattcg
     5701 cactcgccga cgcgttgaaa gccgataccg ccgaagccat aggccgtctg aaaaaacaca
     5761 atatcgatgt ctatattatg agcggcgaca accaaggcac ggtcgaatat gtcgccaaac
     5821 aactgggcat cgcacacgcc ttcggcaata tgagtccgcg cgacaaggct gccgaagtac
     5881 agaaactcaa agccgccggc aaaaccgtgg cgatggtcgg cgacggcatc aacgacgcac
     5941 ccgcgctcgc cgccgccaac gtcagcttcg ccatgaaagg cggtgcggac gttgccgaac
     6001 ataccgcctc cgccacgctg atgcagcatt cggtcaacca gctcgccgat gcactgttgg
     6061 tatcgcaggc gactttgaag aacatcaagc aaaacctgtt tttcgccttc ttctacaata
     6121 tcttgggcat tccactcgcc gcactcggct ttttaaaccc cgtcatcgca ggcgcggcaa
     6181 tggcggcaag ctcggtttcc gtgttgagca acgccttgcg cctgaaacgg gtaaaaatcg
     6241 attgaccgca tataactcgc ccgacagctt ttccgaacgg ataaggctgc cgttgtcgat
     6301 atgccgtctg aatccgtttt tcaaatgatt gatatgaata cagaaaaccg ttctccggaa
     6361 caattcgaca tcccgctctt cctcaaaaac ctgcccaagc tgccgggcgt gtaccgtttt
     6421 tttgacgaag gcggcaaagt cttatacgtc ggcaaagcgg tcaacctcaa gcggcgcgtg
     6481 tccggctatt tccaaaaaaa cgaccattcg ccgcgcatcg cattgatggt aaaacaggtg
     6541 catcatatcg aaaccaccat cacgcgttcc gaagccgaag cactgattct cgaaaacaac
     6601 ttcatcaaag ccttatcgcc gaaatacaat attcttttcc gcgatgacaa aagctatcct
     6661 tatttgatgc tcagcggcca tcaatatccg caaatggcgt attaccgcgg cacgctgaag
     6721 aagccaaatc aatatttcgg cccgtatccc aacagcaacg ccgtgcgcga cagtattcaa
     6781 gtgttgcaaa aagtctttat gctgcgtacc tgcgaagaca gtgtattcga gcaccgcgac
     6841 cgtccttgtc tgctttacca aatcaaacgc tgcaccgcgc cttgtgtagg ccacatcagc
     6901 gaagaagatt atcgcgacag cgtgcgtgaa gcagcaactt tcctcaatgg caaaaccgac
     6961 gaactgacgc gtaccctgca acacaaaatg caggccgccg ccgcgaatct gcaatttgaa
     7021 gaagccgccc gttaccgcga ccaaatccaa gcactcggca ttatgcagag taatcagttc
     7081 atcgacagca aaaacccgaa caaccccaac gatatcgatt tgctcgcgct ggcggtttca
     7141 gacggcatcg tctgcgtaca ctgggtcagc atacgcggtg ggcggcacgt cggagacaaa
     7201 agtttcttcc ccgacaccaa aaacgatccc gagccaaatg ggcaagacta tgccgaagcc
     7261 ttcgtcgccc aacactatct gggcaaaagc aaacctgaca tcatcatcag caacttcccc
     7321 atgcccgacg cattgaaaga agccctagag agcgaacacg gcaaacaaat gcaatttgtc
     7381 accaagacca taggcgaacg caaagtttgg ttgaaaatgg cggagcaaaa cgcacaaatg
     7441 gcgattgcac aacgccgcct gcaacaaagc agccagcaac accgcatcga cgaactcgcc
     7501 aaaatcctcg gtatggattc agacggcctc aaccgcctcg aatgtttcga catcagccac
     7561 acccaaggcg aggccactat tgcgtcctgc gttgtgtacg atgagcaaaa catccagccc
     7621 tcgcaatacc gccgctacaa catcaccacc gccaaaccgg gcgacgacta cgccgccatg
     7681 cgcgaagtgt tgacgcgccg ttacggcaaa atgcaggagg ccgaagccaa cggcgagacc
     7741 gtcaaatggc cggatgccgt gttaattgac ggcggcaaag ggcaaatcgg cgtagccgta
     7801 tcggtatggg aagaactcgg gctgcacatt cctttggtcg gtatcgccaa aggcccggag
     7861 cgcaaagccg gtatggaaga actcatactg ccttttactg gcgaagtctt ccgcctgccg
     7921 cccaacagcc cggccttgca tctattgcaa accgtacgcg atgaatcgca ccgtttcgcc
     7981 attaccggcc accgcaaaaa acgcgacaaa gcccgcgtta cctcctcctt aagcgacatc
     8041 cccggcgtag gcagcaaacg ccgccaagcc ctgcttaccc gcttcggcgg tctgcgcggc
     8101 gtgattgccg ccagccgcga ggacttggaa aaagtggaag gcatcagcaa ggcattggcg
     8161 gaaacgattt acaatcatct gcattagcat gctgcccaag caaaaaccgt ctgtaaaaaa
     8221 tatgatacag taggcaggcg tgccgatata aacctaactt cataacgaat aacgatgatt
     8281 cgacaaaacg gaaaacgacc tgatatgaac aatcccgact taccctatcg gcaggcctta
     8341 gaacgcctgt ctcaaaaaca atataacttt accgaagtcc gccgactgct gacagaagcg
     8401 gcctcggcag gtcatcccgc cgccgcattc gagttggcaa aacacctgat ggacgcggac
     8461 agcccctacc aagaccgcga acaaggtatg gaaatgctcc gcatcgccgc cgaacaggga
     8521 catccctacg cgcgttacaa tctggcatat atccaagaat tggaaggcgc gcgcccggaa
     8581 accctgatac cgctttacag accgttggca gaggaaggac tgcccgaagc acaagtccgc
     8641 ctgatgtacc ttctgtatgc gtcccgacat tttgaagaag ccctgaaatg ggcaaaaacg
     8701 accgcaaaaa acaacaaccc ccacggacaa tatctgcttg cccaatactg ccagtacggt
     8761 acgccaccgg attttgaaac ggcacacctt ctctaccgcg aggctgcagc acaaggcttg
     8821 gcggcggcac aatggcagct cggccttcaa taccgtttcg gacaaggtac accaaccgac
     8881 acgacccaag ctatcaatca tttgcgcgcc gccgcacaac aaggatacat tcccgcctac
     8941 accccgcttg ccgaactcat cctacctacg gctcccgatg aagccgttta ccggttccaa
     9001 caggcggcac aggaaaatga ccccgatgcc catgccgcac ttgccgacat ctacctacaa
     9061 ggcaagcatc tggaaagaaa ccacaaactt gccctgcatc acgccgaagc agccgccgcc
     9121 gaacgccatc ccgaaggttt gcggatactg ggcgacatct gccgctacag tttgggcata
     9181 gcccccgata cggaaaaagc ccggcattat tatcggcagg cagccgaagc cggcagcctt
     9241 tccgcctatc agaaactcat atccgacagc gcgttaaacc atcctaacca atacggcggc
     9301 attaaagatt ccgccatcag gcggcaaagg gcagaacagc tttatcaaaa agcccaagcc
     9361 ctgcattacg gattacaatg cgcgcccgaa tacgcagccg cgctcaaact ctacacagaa
     9421 gccgcagaac tcggacacag caaagcccaa accaatctgg gcagcatgta ttacttcgga
     9481 cagggtatga ccgccgacta caatgaagca cgcaaatggt ttgaaaaagc cgccgcgaaa
     9541 aaagacagta tggcgttcta caacctcgcc tgcatccatt acagcggaca cggtgtcggg
     9601 ccggacaaag aaaaagcctg ccgctacctg caagaagcca taaacaacgg atacgggcaa
     9661 aaaagcgtcc tgcaagaact gttgcaacag tggcaaaatg ccgtctgaac agcgttacac
     9721 ctaccctgcc gaaacgaaac aggtataatc gcccctttcc tttcccgccg tccgaacagg
     9781 cctttcacat tcagacggca tcctgattgc acaagcgcac ggaagcatta tgacagacac
     9841 cgccgaaaac caaacaccaa acgaccgtca agcaggacac ccccgcagca tccgcagctt
     9901 cgtcctccgc caaagccata tgaccgccgc gcagcaacgc gccatcgata ccttatggga
     9961 cagcttcggc atcgactacc aagcaacacc ggccgatctt gatgcccgtt tcggaagcag
    10021 ccgacccaaa atcctcgaaa taggcttcgg tatggggacg gcaaccgcag aaatcgcccg
    10081 ccgcctgccc gaaaccgact tcctcgccat cgacgtacac ggtcccggcg taggcaacct
    10141 gctcaaactc atagacgaaa accatttgga aaatatccgc gtgatgcggc acgatgccgt
    10201 agaagttgtc gaaaatatgc tgcaagacgg ctcgctcgac ggcatccaca tattcttccc
    10261 cgacccgtgg cacaaaaaac gccaccacaa acgccgtctg atacaagccc ccttcatcgc
    10321 caaactactg cccaaactca aaaccggcgg ctatatccac ctggcgacag actgggaaga
    10381 atatgcacag cagatgcttg aagtcctcag tagcttcgac agcctgcaaa atacggcgac
    10441 agactacgcc cctaccccgg actaccgccc cgaaaccaaa ttcgaagcgc gcggcaaacg
    10501 tctcggacac ggcgtttggg acttggtatt caaacggatc agataacaaa ccgctgtttg
    10561 aaaaaatgcc gtctgaagca tgtttgctta cagacggcat tttttcaaga taaagcagcg
    10621 agtgatgttt cgatacaagg tttaaaacaa tggtttgaac gaaaaaacgc atgcgtgcgt
    10681 atcacacata ctgcacgtca gcttgagagt ttgtgcgacc ctcaggctac ccttgctgtt
    10741 gctccaaaac gtatactact tctatactgc gagggtcatt agagtaatta tcctctgttg
    10801 cttgtaaata actgtctgta tcttttatgc ggtcaaataa cttagctcta aattcaccat
    10861 caatttcata aacaaaaaca gcttgtgccc catctggctc acgagataag gcattacttc
    10921 tattactagg cagagcagct ctaactattt ccaaatgatt ttcaggaata tcagatatat
    10981 catttatgtt ttctacacga aaagaatatg tcattttcag tcctcagaat gtaattgatc
    11041 aagttacgat gtttctccac atcttttgtt tctccaaatc ttttgtttct ccaaatcttt
    11101 taagaacatt gtggtcatca actaaacttt caagtgcttt tattactcac tcccaaataa
    11161 caacccttcc ctaatccccc gaatcctatc ccttaacacc gacgcctctt caaactgtaa
    11221 atccctagcc gcctgctgca tggctttttc cagtttggcg atttctttaa tcgcgtcttc
    11281 ttcgttgtga atttcgccga ctttaacttt gtttttgcct tgtctacggc ctttgccgct
    11341 gtcttcttcg tggtacacgc cgtcaatgat gtctttgacc tgttttttaa tctgttgcgg
    11401 cacgatgccg tgttcttcgt tgaatttaat ttgtttttca cggcggcatt cggtttcgtc
    11461 gatggcggct ttcatagagt cggtgatttt gtcggcgtac agaatcgcga cgccgttcac
    11521 gttgcgcgcg gcgcggccta tggtttgaat caggctgcgg tgtgagcgca ggaagccttc
    11581 tttgtcggcg tcgaggatgg cgacgaggga gacttcgggg atgtcgaggc cttcgcgcaa
    11641 gaggttgatg ccgacgagta cgtcaaacag gccgagccgt aaatctctaa tgatttcaac
    11701 gcgctctacg gtatcaatgt cgctgtgcag gtagcgcact ttgatgccga gttcgctgta
    11761 atagtcggtg agttgctccg ccatgcgttt ggtgagggtg gtaacgagaa cgcgttcgcc
    11821 tttttggatg cggtcgttga tttcgctcat taagtcgtcg acttgggtgg cgacggggcg
    11881 gatgatgatt tgggggtcga ccagccctgt ggggcggacg acttgttcga ccacttgtcc
    11941 ggcgtgttct tcttcgtatt tggctggagt ggcggaaacg aagacagttt gcggcatgac
    12001 tttttcaaat tcatggaatt tgagcgggcg gttgtcgcgg gcggaaggca ggcggaagcc
    12061 gtagtcgacg aggttttgct tgcgcgatgc gtcgcctttg tacatgccgc cgatttgggt
    12121 gacggtaacg tggctttcgt cgatgaacat gatggcgttg tcaggcaggt agtccatcag
    12181 cgtgggcggc ggttcgcctt cttttttgcc ggagaagtgc cgggagtaat tttcaatgcc
    12241 tttgcagaag cccatttcgt agagcatttc gaggtcgaaa cgggtgcgct gttcgatgcg
    12301 ttgttgctcg acggggcgtt gttcgcgggc gaaaaattcg atgcgttcgc gcaattcttc
    12361 tttgatggac tcgcaggcgc gcaatacggt gtcgcgcggg gtaacgtagt ggctggacgg
    12421 gaagacggtg tagcggccga cgcgttgaat aaggctgcct gaaagcgggt cgaacatatc
    12481 gaggcggtcg atttcgtcgt cgaacaggct gatacgcaag gcgttttcgg agctttcggc
    12541 ggggtacacg tcaatcacgt cgccgcgcac gcggaagctg ccgcgtttga agtccaaatc
    12601 gccgcgttcg tactgcatgg aaacgagcgt ggcgatgatg tcgcgctgct cgatggtgtc
    12661 gccttctttg acagacaaca ccatttgctg atactcggtc gggtcgccga taccgtaaat
    12721 ggcggacaca gtggcaacga taatcacgtc atcgcgcgtc atcaggtttt tggtggcaga
    12781 aaggcgcatt tgttcaatgt gttcgttgat cgcactgtct ttttcgatga acaaatcgcg
    12841 gcttggcaca taggcttcgg gttggtaata gtcgtagtag gagacgaaat attccaccgc
    12901 gttttcaggg aaaaactcgc gcatttcggc ataaagctgg gcggcaaggg ttttgttgtg
    12961 cgccatgatg atggcgggtc ggccgctttg ggcgatgacg ttcgccatgg tgtaggtttt
    13021 gcccgaaccg gttacgccga gcagggtttg gtaggcaagg ccgtctgaaa gcccttcgag
    13081 caagtcggca atagcggtag gctggtcgcc tgcgggcggg aagggttggt ggagtttgaa
    13141 aggggaatcg gggtatcgga tgacttccat aatcttgcct gtgatgcgtt tgcggacaaa
    13201 gcgtgtagta gggatgggtc ggaaacgtct ttcagacggc ataaggcggt gaaatcctga
    13261 atgtatgccg tctgaaaccc aatcgctacc caa
//
LOCUS       DAXHSJ010000044        12916 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000044 DAXHSJ010000000
VERSION     DAXHSJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12916
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.044"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..1617
                     /locus_tag="ACFM8U_000897"
     CDS             <1..1617
                     /locus_tag="ACFM8U_000897"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6334157.1"
                     /translation="LYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRYTADGKQ
                     AVGEIKRHSDGTPAENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYNTGNDGFKYND
                     KFNHSTWLTFMKNRLEIAKELLMEDGSIFVSIDDNEQAYLKILMDEVFGNENFICNFI
                     WEKKTGASDAKQIATITEFVLCYSKNFKTVKLNKNTFSYDTERYKLSDKFEQERGKYY
                     IDNLDRGGLQYSDSLNFAIQCPDGTFTYPNGRTEFVNDGWIWKWSKNKIDWAITNGFL
                     EFRKSKSKKSGWSVCYKNYMLVDNENKPIERSAPYKNLIQDILNTHATDELKKLFGSK
                     VFTTPKPESLLQYLIQIATSENDIVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETL
                     AVERLKKVIDGEQGGISKAVNWQGGGEFVYAELAPFNETAKQQILACEDSDGIKTLFE
                     GLCERYFLKYNVSINEFSQIIEEPEFQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQ
                     FADCLNDDDKALSRAFYQSVKNQAEKKDGE"
     gene            1607..4390
                     /locus_tag="ACFM8U_000898"
     CDS             1607..4390
                     /locus_tag="ACFM8U_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC6334158.1"
                     /translation="MANNKTLFEVIENERKAVKKYKPELLEMPEFTSKNLKYDFFEWQ
                     KSALENFLIFDRTSKLDDFPDLKNKPTHLLFNMATGAGKTMMMAALILYYFEKGYRHF
                     LFFVNQNNIVDKTENNFIDPTHAKFLFTEKILQGDTVIPIRKVETFSPHSDGIEIKFT
                     SIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKAN
                     AGNAEIERKGWEHMVLELLLNKNGNPSQNVLLEFTATLPENADVQQKYADKIITKFGL
                     KEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKTI
                     DESKADYLAFLNWAENVQAVDFSFLTTFSTSLNDSDSDNANEQGKTRTEQALKFMQEK
                     GVEFAHLADWVKQNYQKHNVIITNSETNKTKTEKTDSETEKLLNNLEAADNPIRAIFT
                     VDRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFE
                     GKQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLA
                     TFKLKSEFAANKDFRELLIWANKKIPNPNAKTNNANSLQANPQTLSFQVHGNQLLQET
                     QFTADENDEKVKQLGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKL
                     NIQNRNELQNKLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIG
                     TKEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISAR
                     LGDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIF
                     IEPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTE
                     SFPLGAASLEKMK"
     gene            complement(4526..5698)
                     /locus_tag="ACFM8U_000899"
     CDS             complement(4526..5698)
                     /locus_tag="ACFM8U_000899"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC6334159.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            6049..6495
                     /gene="iscR"
                     /locus_tag="ACFM8U_000900"
     CDS             6049..6495
                     /gene="iscR"
                     /locus_tag="ACFM8U_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC6334160.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQSGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAVPPAQINIAQIISAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6524..7738
                     /locus_tag="ACFM8U_000901"
     CDS             6524..7738
                     /locus_tag="ACFM8U_000901"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC6334161.1"
                     /translation="MTIKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYVNGDLEHRAPNNLNISFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            7805..7933
                     /locus_tag="ACFM8U_000902"
     CDS             7805..7933
                     /locus_tag="ACFM8U_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334162.1"
                     /translation="MNEQDLDLDNLDNLLDDFDGVTVEGGVDSENDDGCEGGACKI"
     gene            7999..8385
                     /gene="iscU"
                     /locus_tag="ACFM8U_000903"
     CDS             7999..8385
                     /gene="iscU"
                     /locus_tag="ACFM8U_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC6334163.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            8472..8792
                     /gene="iscA"
                     /locus_tag="ACFM8U_000904"
     CDS             8472..8792
                     /gene="iscA"
                     /locus_tag="ACFM8U_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC6334164.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(8752..8973)
                     /gene="arfA"
                     /locus_tag="ACFM8U_000905"
     CDS             complement(8752..8973)
                     /gene="arfA"
                     /locus_tag="ACFM8U_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue factor ArfA"
                     /protein_id="HFC6334165.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNGQE
                     VKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            9055..9555
                     /gene="hscB"
                     /locus_tag="ACFM8U_000906"
     CDS             9055..9555
                     /gene="hscB"
                     /locus_tag="ACFM8U_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC6334166.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            9653..12403
                     /gene="gyrA"
                     /locus_tag="ACFM8U_000907"
     CDS             9653..12403
                     /gene="gyrA"
                     /locus_tag="ACFM8U_000907"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC6334167.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            12560..12916
                     /locus_tag="ACFM8U_000908"
                     /pseudo
     CDS             12560..12916
                     /locus_tag="ACFM8U_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3902 a         3236 c         2877 g         2901 t
ORIGIN      
        1 ttatacacca aattaacccg aaaaagacaa gaaatctttt ttaatcaaac acttgctttt
       61 gatgaaatag ataggctttt tgacgcaaaa gcattctcaa aattctctcg ctataccgca
      121 gacggcaaac aagcggttgg cgaaatcaaa cgccattcag acggcacacc ggccgaaaat
      181 cttattatca aaggcaataa tctgattgcc ctgcattcgc ttgccaaaca gtttaaaggc
      241 aaagtgaagt tgatttatat tgatccgcca tataacacgg gcaatgacgg tttcaaatac
      301 aacgacaaat ttaatcattc cacttggctg acttttatga aaaaccgtct agaaatcgcc
      361 aaagagctgc ttatggaaga cggttcgatt tttgtgtcaa ttgacgacaa cgaacaggca
      421 tatttgaaaa ttttaatgga tgaagttttc ggaaatgaaa atttcatctg caattttatt
      481 tgggaaaaaa agacaggtgc gtccgatgcc aaacagatag cgactattac agagtttgtc
      541 ttatgttact caaagaactt taaaacagtt aaattaaata aaaacacgtt ttcttatgat
      601 acagagagat acaaattaag tgataagttt gaacaggaaa gaggcaaata ttatatcgac
      661 aatttagata gagggggatt gcagtatagt gacagtttga attttgcaat ccaatgtcca
      721 gatggcactt ttacgtatcc gaatggcagg actgaatttg tcaatgatgg ctggatatgg
      781 aaatggagta aaaataaaat tgattgggca ataacaaacg gttttttgga gtttagaaaa
      841 tcaaagtcta aaaaatctgg atggagtgta tgttataaaa actatatgtt ggttgataac
      901 gaaaacaagc cgatagaacg ttctgctccc tataagaact taatacagga tatcttaaat
      961 acacatgcga cagatgaatt gaaaaaactg ttcggcagca aagtttttac tactccaaaa
     1021 cctgagagct tattgcagta tcttattcaa attgccacat ccgaaaacga catcgtccta
     1081 gactaccatt taggcagtgg cacaaccgcc gccgttgccc acaaaatgaa ccgccaatat
     1141 atcggtattg aacaaatgga ttatattgaa acgcttgccg ttgaacgctt gaaaaaagtg
     1201 attgatggcg agcaaggcgg tatttccaaa gccgtgaatt ggcaaggtgg tggcgaattt
     1261 gtttatgccg aacttgctcc atttaacgaa accgcaaaac aacagatttt ggcttgcgaa
     1321 gattcagacg gcatcaaaac gctgtttgaa ggtttatgcg aacgctattt cttgaaatac
     1381 aacgtcagca taaatgaatt tagccaaatt attgaagagc ctgaatttca atctttgcca
     1441 ttagacgaac aaaaacaaat ggtgcttgaa atgttggatt taaatcaaat gtatgtttca
     1501 ttatccgaaa tggatgacga acaatttgca gattgcctga acgatgatga taaagcctta
     1561 agccgtgcat tctatcaatc agtaaaaaat caagcggaga aaaaagatgg cgaataataa
     1621 aacgttgttt gaagtgattg aaaatgaacg taaagcggtt aaaaaataca agcctgaatt
     1681 acttgaaatg ccagaattta cgtccaaaaa cttaaaatat gatttttttg aatggcaaaa
     1741 atctgccctt gaaaactttt tgatttttga ccgcacttca aagctagacg atttccctga
     1801 tttaaaaaat aagccaacgc atttgctgtt caatatggca acaggtgcag gcaaaacgat
     1861 gatgatggcg gcattgattt tgtattattt tgaaaaaggg tatcggcatt ttctgttttt
     1921 tgtgaatcaa aacaatatcg tggataaaac ggaaaataat tttattgacc cgacacacgc
     1981 aaaattttta tttaccgaga agattttgca gggcgatacg gtaattccca ttcgcaaagt
     2041 ggaaacgttc agcccacatt cagacggcat tgaaattaaa tttaccagca ttcaaaagct
     2101 gtataacgat attcacaccg aacgggaaaa ccaaaccaca ttggcggatt tgcacgaatt
     2161 gaatcttgtg atgctgggcg atgaagcaca ccatttaaac gcgcaaacca aaggcaaaaa
     2221 acaaggcgag ttggatttgg aagtggaatt aaaagccaat gcaggcaatg ccgaaattga
     2281 acgcaaaggc tgggagcata tggttttgga attgttactc aataaaaatg gcaatcccag
     2341 ccaaaatgtg ctgttggaat ttaccgccac gctgcctgaa aatgctgacg tgcaacaaaa
     2401 atacgcggat aaaatcatca caaaatttgg cttaaaagaa tttttgcaaa aaggttatac
     2461 caaagaaatc aatttggtat ccagtacgct gggcaagaaa gagcgggtgt tgcacgcttt
     2521 attatttgct tggtatcgcc atcaaatcgc tttgaaatat ggcattgcca atttcaagcc
     2581 tgtgatgttg tttagaagta agacgattga tgaatcaaaa gcggattatc tggcattttt
     2641 aaattgggca gaaaatgtgc aggcggttga tttttcgttt ttaactacat tttcaacaag
     2701 cttgaacgat agcgatagcg ataacgccaa cgaacaaggc aaaacccgca ctgaacaagc
     2761 cctaaaattt atgcaggaaa aaggcgttga gtttgcacat ttggcagatt gggtaaaaca
     2821 aaattatcaa aaacacaatg tgattattac caactccgaa accaacaaaa ccaaaaccga
     2881 aaaaaccgac agcgaaacag aaaaattgct gaataatttg gaagcggctg ataatccgat
     2941 tcgtgccatt tttacggtgg acagattaac cgaaggttgg gacgttctga atttgtttga
     3001 tattgtgcgt ttgtatgaag ggcaaaacgg cggcggttca aataaaaaat caggcaaaac
     3061 ggctgccgcc accgtatcgg aaaagcagtt gattggtcgt ggcgtgcgtt attttccatt
     3121 tgcgtttgaa ggtaaacagc cgaataaacg caaatttgac aacgatatgc aacacgaatt
     3181 gcgtatcttg gaagaattgt tttattacac gcacgatgag caatcccgct atatttcaga
     3241 actgaaaaac gagttgcgaa aagacggtta tttgcctgaa aaagacgatg ataaggtatt
     3301 ggcaacattt aaactcaaat ctgaatttgc cgctaataag gattttagag aattgttaat
     3361 ttgggcgaat aaaaaaatcc ccaacccaaa cgccaaaacc aataatgcaa acagcctgca
     3421 agccaatccg caaacgcttt cattccaagt tcacggcaat caactgttgc aggaaacgca
     3481 atttacagcc gatgaaaatg atgaaaaagt caagcaactt ggcacgcaaa ataattttac
     3541 tcaaaccata aaaatgagtg aaatggagcg gcacattttc aataaagccc tgcatatcaa
     3601 agggaaaaat agtcaatctt tattccattt tgaccgcctg caaagcaaac tcaatattca
     3661 aaatcgcaat gaattgcaaa ataaattgtt gaaagactgg caaattgaat ttttaggatt
     3721 agggcaagac aaacaggttc gcctggacga taaacttgca ggctgcctga aaatcttgga
     3781 aatggttgaa aaacatctga atgaaagtga tatgccgttt atcggtacaa aagaatttac
     3841 acctaaaaaa ttatgggaaa ttttcggcac gccaaaacaa aaatgggtca aaaaagatga
     3901 tgtaaaaacg actattgcca cgcaaaatga ttggtatgtg atgaataatt ttgccggaac
     3961 gggtttggaa gaagcgttaa ttcaatttat ttcagcgcgt ttgggcgatt tgaaatctca
     4021 atatgacgtt catttaatcc gtaatgaaga agtgtttaaa ttgaataact ttgccgatgg
     4081 cgaaggattt atgccggact ttattttatt gctgaaagat aaacaaaaat cttcttccaa
     4141 tggtgtgaac gactttttgc attaccaaat tttcattgag cccaaaggtg agcatttagt
     4201 ggaaactgat ggatggaaag aagaattttt agaagccatc actgtggaat atgggatgga
     4261 taagatcctg caaaaagata caccgcatta tcgtttgatc ggtttaccgt tttttactga
     4321 tcatcagaaa aatggacaat ttacggagtc attcccttta ggtgcggcat cgcttgaaaa
     4381 aatgaagtag tgcattgcag gcaaccccgt ttgacaaaac ttcctttaca aaagggcgtt
     4441 ttgtcagata gttcattatt aaaatacagc caaattttaa tgccgtccga accctgtgtt
     4501 cagacggcat cgtatttttc agtatctaaa ccgtttccct gccccaatct ttacccctta
     4561 aaatcgaggc atcgacatct tgaatatcgc ggtgccccgt aaacgccata gatatatcca
     4621 tttccttata taggatttcc agcgcgcggg ttacgccttc ttctccatac gcgcccaagc
     4681 catacagaaa cgcccgacct atcattgtac ctttcgcgcc caaagcccac gccttcaaaa
     4741 tatcctgacc gctgcggatg ccgctgtcca tccaaacttc gatgtcgctg cccactgcgc
     4801 tgacgatgtc gggcaaggct ttgatggcag acacggtatc gtcgagctgt cggccgccgt
     4861 ggttggaaac gaccaatgcg tccgcgccgc ttttcgctgc tttttccgcg tcttcgggtt
     4921 ccataatgcc tttgataatc agcttgccgc cccacaaatc tttaatgcgc gccacatcgt
     4981 cccagctcag gcgcgggtcg aattgttcgg aagtccatga agacagcgaa gacaaatcgc
     5041 cgacgttctt cgcgtgtccg acgatattgc ggaacgtgcg gcgttccgtg ttcagcattt
     5101 tcatgcacca ttccggtttg gtcgccagat tgattaaatt ggcgatggtc ggtttcggcg
     5161 gcgcggacag gccgtttttg atgtctttgt ggcgttgacc caaaacctgc aaatcggcgg
     5221 tcaataccaa tgccgaacat ttggcatcct tcgcgcgctt aatcaggttt tccataaact
     5281 cgcggtcgcg catcacataa agctgaaacc aaaatggtgc ggaagtgttc tcggcaacgt
     5341 cttcaatcga gcagatggac atggtggaca acgtaaacgg aatgccgaac ttctccgccg
     5401 cccgcgccgc caagatttca ccgtcggcgt gtgccatgcc ggtaaaacca gtcggggcaa
     5461 tcgccaccgg cattttcaca tcctgcccga tcattttggt ttccaggctt cgaccttcca
     5521 tattgaccaa taccttttgt cggaagcgga tatctttaaa atccgaagtg ttttcgcggt
     5581 aggtagtttc cgtccacgaa cccgaatcga tgtaatcgta aaacatacgc ggcatcttgc
     5641 gtttggcaac gcggcgcaag tcttcgatgc aggtcatttt gctcaaatca cgtttcatct
     5701 tgtttttccc tgtttgacag cctgatggaa tatgccgtcc gaaaccgctt gagccttcag
     5761 acagcatttc aggcaatacc caaataaatc actaagattt agataatcta tatcaatatc
     5821 aaaaataaat caaatcgttt tgaaaccgct tgccggcaaa ataccccgac caccgctcct
     5881 caaaaaacat aaccaactga tttaaaaaac caaacaaata aagcaaaatc agactatacc
     5941 gccatttaaa caaacaatcg gcaacagctc ccccttactt gactgaaaca ctcagatatt
     6001 ggacaattcc acccactaat aaaaaaaccg acatgggcaa ccaccaccat gagactgacc
     6061 accaaagggc gtttcgccgt taccgctatg ctggatttgg cgatgaacgc gcaaagcggc
     6121 gccgtcaaac tcagtgccat cagcgaacgc caaaacatat ccctctccta tctcgagcaa
     6181 ttgttcggca aactccgccg tgccggactg gttgaaagcc tgcgcgggcc cggcggcggc
     6241 tacatcctcg ctgtccctcc cgcacaaatc aacatcgccc aaatcatttc cgccgccgaa
     6301 gaccggctgg acgcgaccca atgcggcagc aaagccaact gccaccacgg cgcgccctgc
     6361 ctgacgcacg acctttggga gaatttaaac aaaaccatca acgactacct cggcagcgtt
     6421 accctgcaaa gcatcatcga acagaaaaat aacggcgacg gcagccgcgt cgtccaattt
     6481 acacacatcc attaaataac acccgaaaaa gaaagagcaa accatgacca tcaaaacccc
     6541 cgtttacctc gactacgccg ccaccacacc cgttgacaaa cgcgttgccg aaaaaatgat
     6601 tccctatctg accgaaacct tcggcaaccc cgcctccaac agccacgcat tcggctggga
     6661 agcagaagag gccgtcgaaa aagcccgcgc cgacatcgcc gccctgatta atgccgaccc
     6721 caaagaaatc gtcttcacca gcggcgcgac cgagtccgac aacctcgcca tcaaaggtgc
     6781 ggcaaacttc tacaaaacca aaggcaaaca cctcatcacc gtcaaaaccg aacacaaagc
     6841 cgtactcgac acgatgcgcg aactcgaacg ccaaggtttt gaagtaacct acctcggcgt
     6901 gcaggaaaac ggtttgattg atttggaaga actcaaagcc gccatccgcg acgacaccat
     6961 cctgatttcc gtaatgtggg caaacaacga aatcggcgta gtgcaagaca ttcccgccat
     7021 cggcgaaatc tgccgcgaac gcaaaatcgt cttccacgtc gatgccgccc aagcctgcgg
     7081 caaagtacct gtcgatgtcg aagccgccaa aatcgacctg ctctcgatgt ccgcgcacaa
     7141 agtgtacggc cccaaaggca tcggcgcgct gtacgtccgc cgcaaaccgc gcgtccgcct
     7201 cgaagcccaa atgcacggcg gcggccacga acgcggcttc cgcagcggca cattgccgac
     7261 ccaccaaatc gtcggtatgg gcgaagcctt ccgcatcgcc aaagaagaac tcgaacaaga
     7321 tatggcgcac taccgcaaac tgcgcgacat cttcctcaaa ggcatcgaag gcatcgaaga
     7381 agtctatgtc aacggcgacc tcgaacaccg cgccccgaac aacctgaaca tcagcttcaa
     7441 cttcgtcgaa ggcgaaagcc tgattatggc ggtcaaagaa ctcgccgtat ccagcggctc
     7501 cgcctgcacc tccgcctcgc tcgaacccag ctacgtcctg cgcgcgctcg gccgcaacga
     7561 cgaactggcg cactcatccc tgcgcatcac cttcggccgc atgaccaccg aagaagaagt
     7621 gcaattcgcc gccgaactga ttaaatccaa aatcggcaaa ctgcgcgaac tgtcgccgct
     7681 gtgggaaatg ttcaaagacg ggattgattt gaattcgatt gagtgggcgg cgcattaaag
     7741 cgtaccaaca tgccgtccga acctttagac ggcattccaa aaacaaagca atcaagagaa
     7801 aaatatgaac gaacaagatt tagatttgga caacctcgac aacctgcttg acgattttga
     7861 cggcgttacc gtcgaaggcg gcgtcgattc ggaaaacgac gacggctgtg aaggcggcgc
     7921 gtgcaaaatc taactcaagc cgtctgaaaa ccggcaaaca aaccacttaa atcatcaaaa
     7981 cattaaggaa accacatcat ggcatacagc gataaagtaa tcgaccacta cgaaaatccc
     8041 cgcaacgtcg gcactttcga caaaaacgac gagtccgtcg gcaccggcat ggtcggcgca
     8101 ccggcctgcg gcgacgtgat gcgcctgcaa atcaaagtga acgatgaagg catcatcgaa
     8161 gatgcgaaat tcaaaactta cggctgcggt tccgccatcg cttcgtccag cctgattacc
     8221 gagtgggtca aaggcaaaag cctggatgac gcgctggcaa tcaaaaacag cgaaatcgcc
     8281 gaggagttgg aactgccgcc ggtaaaaatc cactgctcca tcttagcgga agacgcggta
     8341 aaagcggccg ttgccgacta ccgcaaacgt caggaaaaca gataaagccc ttcagacggc
     8401 atcgtcccgc aatgccgtcc gaaccgcccg ccgcttcggg ttcgtccgag gcggtacaac
     8461 aaggaagaaa tatgattacc cttaccgaga atgccgcaaa acacatcaat gactatctcg
     8521 ccaaacgcgg caaaggcttg ggcgtacgct tgggtgtgaa aaccagcggc tgctcgggga
     8581 tggcgtacaa ccttgaattt gtcgatgaag ccgatggcga cgacctgatt ttcgaaggac
     8641 acggcgcacg catttatatc gacccgaaaa gcctggttta tctggacggc acacaagtcg
     8701 attacaccaa agaaggtttg caggaaggtt tcaaatttga aaaccccaat gtcaaagact
     8761 cctgcggctg cggcgaaagc ttccatgttt aagacataaa aatggcggga ccgtatcaaa
     8821 accgtcccgc cattttttca cttcctgccc gttgtagctg cctttgcctt tccttttccg
     8881 ttccaccttg tgccggaaca aatcggattt cactaaggct tttaaagcat tgtcgcgtat
     8941 tttgccttta ttgtgctgca ctttgccgcc catattcagt cctttcgttt aagaagctgc
     9001 agattataag gcaaaaacag ttttctgcca aaatcttaca tttatcatcc tactatgtcc
     9061 caatatttca ccctcttccg gattgaaccc gctttcgata tcgacaccga aaacttggaa
     9121 caaacctacc gcgccttggc cgcccgtttc catcccgata aattcgcttc agcttccgcc
     9181 tttgagcaaa agcaggcagt gatgatgtct tccaccatca acgatgccta ccgcaccttg
     9241 aaaaacccca tcgaccgcgc cgcctacctg ctgaaaacat cgggcatcga tgccgacgcg
     9301 ccggagcata ccgctttcgc ccccgaattc cttatgcagc aaatggaatg gcgcgaaacg
     9361 ctgatggagg cacgggcagg caacgacctt gaatccttga aaaatctcga caacgaaatc
     9421 cgcgacgaac aagaaaaact gttctgcggt ctgaaacagt cgtttgcccg acaagattac
     9481 gacacagccg cacaacaagt ccgccaaggc aggtttctcg acaaactccg caacgaaatt
     9541 tcctcggcat tataatccgc accgtgtttc agacggcgta accgccgcac cgttccgcgt
     9601 caaaatatgc taaaataagc aacaattttt tgccatacga aacattgaaa ccatgaccga
     9661 cgcaaccatc cgccacgacc acaaattcgc cctcgaaacc ctgccggtaa gccttgaaga
     9721 cgaaatgcgc aaaagctatc tcgactacgc catgagcgtc attgtcgggc gcgcgctgcc
     9781 ggacgttcgc gacggtctca agccggtaca ccgccgcgta ctgtacgcga tgcacgaatt
     9841 gaaaaacaac tggaatgccg cctacaaaaa atcggcgcgc attgtcggcg acgtcatcgg
     9901 taaataccac ccccacggcg ataccgccgt atacgacacc atcgtccgta tggcgcaaaa
     9961 tttcgctatg cgttatgtgc tgatagacgg acagggcaac ttcggatcgg tggacgggct
    10021 tgccgccgca gccatgcgct acaccgaaat ccgcatggcg aaaatttccc acgaaatgct
    10081 ggcagacatt gaggaagaaa ccgtcaattt cggcccgaac tacgacggta gcgaacacga
    10141 gccgcttgta ctgccgaccc gtttccccac actgctcgtc aacggctcgt ccggcatcgc
    10201 cgtcggcatg gcgaccaata tcccgccgca caacctttcc gataccgtca atgcctgcct
    10261 gcgcctgctc gatgcacccg acaccgaaat cgacgaactg atcgacatta tccaagcccc
    10321 cgacttcccg accggggcaa ccatctacgg cttgagcggc gtgcgcgaag gctataaaac
    10381 aggccgcggc cgcgtcgtta tgcgcggtaa gacccatatc gaacccatag gcagaaacgg
    10441 cgaacgcgaa gccatcgtta tcgacgaaat cccctatcag gtcaacaaag ccaagctggt
    10501 cgagaaaatc ggcgatttgg ttcgggaaaa aacactggaa ggcatttccg agctccgcga
    10561 cgaatccgac aaatccggta tgcgcgtcgt tatcgagctg aaacgcaacg aaaatgccga
    10621 agtcgtctta aaccaactct acaaactgac tccgctgcaa gacagtttcg gcatcaatat
    10681 ggtggttttg gtcgacggac aaccgcgcct gttgaacctg aaacagattc tctccgaatt
    10741 cctgcgccac cgccgcgaag tcgttacccg acgtacgctt ttccggctga agaaggcacg
    10801 ccatgaaggg catattgccg aaggcaaagc cgtcgcactg tccaatatcg atgaaatcat
    10861 caagctcatc aaagaatcgc ccaacgcagc cgaggccaaa gacaaactgc ttgcgcaccc
    10921 ttggcgcagc agcctcgttg aagaaatgct gacgcgttcc ggtctggatt tggaaatgat
    10981 gcgtccggaa ggattggctg caaacatcgg cttgaaagag caaggttatt atctgagcga
    11041 gattcaggca gatgctattt tacgcatgag cctgcgaaac ctgaccggcc tcgatcaaga
    11101 agaaattgtc gaaagctaca aaaacctgat gggtaaaatc atcgactttg tggatatcct
    11161 ctccaaaccc gaacgcatta cccaaatcat ccgcgacgaa ctggaagaaa tcaaaaccaa
    11221 ctatggcgac gaacgccgca gcgaaatcaa cccgttcggc ggcgacattg ccgatgaaga
    11281 cctgattccg caacgcgaaa tggtcgttac cctgacacat ggcggctata tcaaaaccca
    11341 gccgaccacc gactatcagg cgcagcgtcg cggcgggcgc ggcaaacagg cggctgccac
    11401 caaagacgaa gactttatcg aaaccctgtt tgttgccaac acgcatgatt atttgatgtg
    11461 ctttaccaat ttgggcaagt gtcattggat taaggtttac aaactgcccg aaggcggacg
    11521 caacagccgc ggccgtccga ttaacaacgt catccagttg gaagaaggcg aaaaagtcag
    11581 cgcgattctg gcagtacgcg agttccccga agaccaatac gtcttcttcg ccaccgcgca
    11641 gggaatggtg aaaaaagtcc aactttccgc ctttaaaaac gtccgcgccc aaggcattaa
    11701 agccatcgcg ctcaaagaag gcgactacct cgtcggcgct gcgcaaacag gcggtgcgga
    11761 cgacatcatg ctgttctcca acttaggtaa agccatccgc ttcaacgaat actgggaaaa
    11821 atccggcaac gacgaagcgg aagatgccga catcgaaacc gaaatttcag acggcatcga
    11881 agatgaaacc gccgacagcg aaaacgcact gccgagcggc aaacacggtg ttcgcccgtc
    11941 cggtcgcggc agcggcggtt tgcgcggtat gcgcctgcct gccgacggca aaatcgtcag
    12001 cctgattacc ttcgcccctg aaaccgaaga aagcggtttg caagttttaa ccgccaccgc
    12061 caacggatac ggaaaacgca ccccgattgc cgattacagc cgcaaaaaca aaggcgggca
    12121 aggcaatatt gccattaaca ccggcgagcg aaacggcgat ttggtcgccg caaccttggt
    12181 cggcgaaacc gacgatttga tgctgattac cagcggcggc gtacttatcc gcaccaaagt
    12241 cgaacaaatc cgcgaaaccg gccgcgccgc agcaggcgtg aaactgatta acttggacga
    12301 aggcgaaacc ttggtatcgc tggaacgtgt tgccgaagac gaatccgaac tctccgacgc
    12361 ttctgtaatt tccaatgtaa ccgaaccgga agtcgagaac tgaaaatcat ctcccgatgc
    12421 cgtctgaaga ttcagacggc atttatttta tccctcatcc gtcatccagc ttctcacaat
    12481 atagcggatt atagtcaatt aaaaacaagg ggctgtccta gataactagg gaaattcaaa
    12541 ttaagttaga gttgccccta tgagaaaaag tcgtctaagc cggtataaac aaataaactc
    12601 attgaactgt ttgtcgcagg tgtaactgca agaacgacag cagagttagt aggcgttaat
    12661 aaaagtaccg cagcctatta ttttcatcgt ttacgattac ttatttatca aaacagtccg
    12721 catttggaaa tgtttgatgg cgaagtagaa gcagatgaaa gttattttgc tgaacgacaa
    12781 aaccatatca atggaattga gaacttttgg aaccgggcaa aacgtcattt acgcaagttt
    12841 gacggcattc ccaaagcgca ttttgagctg tatttaaagg ggtacgaacg gcgttttaac
    12901 aacagtgaga taaaag
//
LOCUS       DAXHSJ010000045        12882 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000045 DAXHSJ010000000
VERSION     DAXHSJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.045"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            25..1107
                     /gene="mraY"
                     /locus_tag="ACFM8U_000909"
     CDS             25..1107
                     /gene="mraY"
                     /locus_tag="ACFM8U_000909"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC6334168.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            1222..1494
                     /locus_tag="ACFM8U_000910"
     CDS             1222..1494
                     /locus_tag="ACFM8U_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFC6334169.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGLAQAQ
                     YSLGAMYYKGRGVRQDLVLAQEWLGKACQNGNQDGCDNDQRLKAGY"
     gene            1582..2919
                     /gene="murD"
                     /locus_tag="ACFM8U_000911"
     CDS             1582..2919
                     /gene="murD"
                     /locus_tag="ACFM8U_000911"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC6334170.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQV
                     LNADDVFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            3056..4369
                     /locus_tag="ACFM8U_000912"
     CDS             3056..4369
                     /locus_tag="ACFM8U_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC6334171.1"
                     /translation="MTGGFLKISEVLVKVGDGVHTLLLDRQIVRDGRKFDAPLLWMVV
                     LMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRL
                     VPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRRE
                     EVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLI
                     MVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAF
                     LDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFG
                     FFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGA
                     LPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            4373..5440
                     /gene="murG"
                     /locus_tag="ACFM8U_000913"
     CDS             4373..5440
                     /gene="murG"
                     /locus_tag="ACFM8U_000913"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC6334172.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            5595..7004
                     /gene="murC"
                     /locus_tag="ACFM8U_000914"
     CDS             5595..7004
                     /gene="murC"
                     /locus_tag="ACFM8U_000914"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC6334173.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            7117..8031
                     /locus_tag="ACFM8U_000915"
     CDS             7117..8031
                     /locus_tag="ACFM8U_000915"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC6334174.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVMGVGFADLCIEILKTAHVG"
     gene            8021..8749
                     /locus_tag="ACFM8U_000916"
     CDS             8021..8749
                     /locus_tag="ACFM8U_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC6334175.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            8835..10079
                     /gene="ftsA"
                     /locus_tag="ACFM8U_000917"
     CDS             8835..10079
                     /gene="ftsA"
                     /locus_tag="ACFM8U_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC6334176.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            10198..11376
                     /gene="ftsZ"
                     /locus_tag="ACFM8U_000918"
     CDS             10198..11376
                     /gene="ftsZ"
                     /locus_tag="ACFM8U_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC6334177.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            11515..12825
                     /locus_tag="ACFM8U_000919"
     CDS             11515..12825
                     /locus_tag="ACFM8U_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC6334178.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
BASE COUNT         2870 a         3259 c         3695 g         3058 t
ORIGIN      
        1 gacaaaacaa aaaaaggaag ccccatgttt ttatggctcg cacatttcag caactggtta
       61 accggcctga atatttttca atacaccaca ttccgcgccg tcatggcggc gttgaccgcc
      121 ttggcgtttt ccctgatgtt cggcccgtgg acgatacgca ggctgaccgc gctcaaatgc
      181 gggcaggcag tgcgtaccga cggtccgcaa acccacctcg tcaaaaacgg cacgccgacg
      241 atgggcggtt cgctgattct gaccgccatt accgtgtcca ccctgttgtg gggcaactgg
      301 gcgaacccgt atatctggat tctcttgggc gtactgcttg ccaccggtgc gctcggtttt
      361 tacgacgact ggcgcaaagt cgtctataaa gaccccaacg gcgtgtccgc caaattcaaa
      421 atggtgtggc agtcaagcgt tgccattatc gccggtttgg cattgtttta ccttgccgcc
      481 aattccgcca acaatatttt gattgtcccg ttcttcaaac aaatcgccct gccgctgggc
      541 gtggtcggct ttttggtgtt gtcttacctg accatcgtcg gcacatccaa tgccgtcaac
      601 ctcaccgacg gcttggacgg ccttgcgacc ttccccgtcg tcctcgttgc cgccggcctc
      661 gccattttcg cctacgtcag cggacactac caattttccc aatacctcca gcttccctat
      721 gtcgccggcg cgaacgaagt cgctatattc tgcaccgcca tgtgcggcgc gtgcctcgga
      781 tttttgtggt tcaacgccta tcccgcgcaa gtctttatgg gcgatgtcgg cgcattggca
      841 ttgggtgccg cgctcggtac cgtcgccgtc atcgtccgcc aagagtttgt tttagtgatt
      901 atgggcggtc tgttcgtcgt agaagccgta tccgttatgc ttcaggtcgg ttggtataag
      961 aaaaccaaaa aacgcatctt cctgatggcg cccatccatc accactacga acaaaaaggc
     1021 tggaaagaaa cccaagtcgt cgtccgcttt tggattatta ccatcgtctt ggtgttaatc
     1081 ggcttgagta ccctcaaaat ccgctgaacc tatgccgttt gaacatcttt cagacggcat
     1141 ttgaacgcgc aataaacctg cggcgacaat ccgcccagcc ctatcgttaa cggcggctta
     1201 tactaaaaca gaagtaaaac catgaaacag acagtcaaat ggcttgccgc cgccctgatt
     1261 gccttgggct tgaaccaagc ggtgtgggcg gatgacgtat cggattttcg ggaaaacttg
     1321 caggcggcag cacaggggtt agcccaagcc caatacagtt tgggcgcaat gtattacaaa
     1381 ggacgcggcg tgcgccaaga ccttgtgctc gctcaagaat ggcttggcaa ggcgtgtcaa
     1441 aacggaaacc aagacggctg cgacaatgac caacgcctga aggcgggtta ttaatcggct
     1501 cgcgatgccg tctgaaagcg gcttgagcag gagcggacat ccccccctgc tcaatatgat
     1561 ttgttttagg gcaaaaccaa aatgactttc caaaacaaaa aaatccttgt cgccggactt
     1621 ggcggcacgg gtatttccat gattgcctac ctgcgcaaaa acggcgcgga ggttgctgcg
     1681 tatgatgcgg agctgaagcc ggaacgcgtg tcgcaaatcg gtaagatgtt tgacgggttg
     1741 gtgttttaca cgggccgtct gaaagatgcg ctggacaacg gtttcgatat tctggctctc
     1801 agtcccggca tcagcgagcg gcagccggat attgaggcgt tcaagcaaaa cggcggacgc
     1861 gtgttgggcg acatcgaatt gctggcggac attgtgaacc gccggggcga caaggtaatt
     1921 gcgattaccg gcagcaacgg caaaaccacg gtaacgagcc tggtcggcta tctctgtatc
     1981 aagtgcgggc tggataccgt tatcgcgggc aatatcggca cgccggtttt ggaggcggaa
     2041 tggcagcgcg aaggcaaaaa ggcggacgtg tgggtgttgg agctttccag cttccaattg
     2101 gaaaacaccg aaagcctgcg cccgacggcg gcgacggtgc tgaatatttc cgaagaccat
     2161 ctcgaccgct acgacgactt gctcgactac gcgcatacca aggacaagat tttccgtggc
     2221 gatggcgtgc aggttttgaa tgcggacgac gtgttctgcc gcgccatgaa gcgcgccggg
     2281 cgtgaagtga aatggttttc gttggaacac gaagccgatt tttggttgga acgcgagaca
     2341 ggccgcctga aacaaggcaa tgaagatttg attgccacgc aagatattcc gttgcaaggc
     2401 ttgcacaacg ctaccaacgt gatggctgcc gttgccttgt gcgaagccgt cggtttgccg
     2461 cgcgaagcat tgctcgaaca cgtcaaaacc tttcaaggcc tgccgcaccg cgtggaaaaa
     2521 atcggcgaga aaaacggcgt ggtgttcatc gacgacagca aaggcacgaa tgtcggcgcg
     2581 accgccgccg cgattgccgg tttgcaaaat ccgctcttcg tgattttagg cggcatgggc
     2641 aaagggcagg acttcacgcc tctgcgcgac gcgcttgccg gcaaggcaaa aggcgtgttc
     2701 ctgatcggcg ttgatgcgcc gcaaatccgc cgcgatttgg acggctgcgg tctgaatatg
     2761 accgactgcg ccactttgga agaagccgtt cagacggcat atgcccaagc cgaagcgtgc
     2821 gatatcgtgc tgctcagccc ggcttgtgcg agttttgata tgtttaaagg ctatgcgcac
     2881 cgttcggaag tgtttatcga agcgtttaag gctttgtgat gccgtctgaa atgcaaacgc
     2941 cgtcattgtt gggcggcaag taaagattta gaataccgat ttgggatgta tcgtatgttc
     3001 ggacggcatt gtctgccgtc tgaaattttt gccctttgcg gcaggtgcaa acagaatgac
     3061 aggtggtttt ttgaagattt cggaagtatt ggtaaaagtg ggcgacggtg tccacactct
     3121 gctgctcgac aggcagattg tgcgcgacgg caggaaattc gacgcgccgc ttttgtggat
     3181 ggtggtgctg atgacggcgt tcagcctgct gatgatttat tcggcttctg tgtatttggc
     3241 atcaaaagaa ggcggcgatc agtttttcta tttgaccaga caggcggggt tcgtcgttgc
     3301 cggcttgata gcgagcggtt ttttatggtt tctttgcagg atgaggacat ggcggcggct
     3361 tgtgccgtgg atttttgcct tatccggcct gttgctggta gtcgtattga ttgccgggcg
     3421 cgaaatcaat ggcgcgaccc gttggatacc tttgggtccg ttgaatttcc agccgaccga
     3481 gctgttcaag ctggcggtca tcctttattt ggcaagcctg ttcacgcgcc gtgaagaagt
     3541 gttgcgcagc atggaaagtt tgggttggca gtcgatttgg cgggggacgg ccaatctgat
     3601 catgtccgcc accaatccgc aggcacgtcg tgaaacatta gaaatgtacg gccgtttccg
     3661 ggcgatcatc ctgccgatta tgctggtggc gttcggtttg gtgctgataa tggtacagcc
     3721 ggatttcggt tcgtttgtcg tcattaccgt cattgccgtt ggaatgctgt ttttggcagg
     3781 attgccgtgg aaatattttt tcgtcctggt aggcagcgtc ttgggcggga tggtgctgat
     3841 gattaccgcc gctccctacc gtgtgcagcg ggtagtggca tttttggacc cgtggaaaga
     3901 cccgcagggt gccggctacc agcttaccca ctctctgatg gcaatcgggc gcggagagtg
     3961 gttcggtatg ggtttgggtg cgagtttgag caaacgcggc tttctgccgg aagcgcatac
     4021 cgattttatt tttgccatca tcgccgaaga attcggtttc ttcggtatgt gcgtgctgat
     4081 attctgttac ggctggctgg tggtgcgggc gttttccatc ggcaagcagt cgcgcgattt
     4141 gggtttgact ttcaacgcct atatcgcttc gggtatcggc atttggatcg gtatccaaag
     4201 tttcttcaat atcggtgtga acatcggtgc tttgccgacc aaaggtctga cgctgccgtt
     4261 gatgtcctat ggcggttcgt cagtcttttt catgctgatc agcatgatgc tgctgttgcg
     4321 tatagattat gaaaaccgcc ggaaaatgcg cggttatcgg gtggagtaaa tcatgggcgg
     4381 taaaaccttt atgctgatgg cgggcggaac gggcggacat attttccccg cgctggcggt
     4441 ggcggattcg ttgcgcgcgc gcggccatca tgtgatttgg ctgggcagca aggattcgat
     4501 ggaagagcgt atcgtgccgc aatacggcat acgcttggaa acgctggcga ttaaaggcgt
     4561 gcgcggcaac ggcatcaaac gcaaactgat gctgccggtt actttgtatc aaaccgtccg
     4621 cgaagcgcag cggattatcc gcaaacaccg tgtcgagtgc gtcatcggct tcggcggctt
     4681 cgttaccttc cccggcggtt tggcggcgaa gctattaggc gtgccgattg tgattcacga
     4741 gcaaaacgcc gtggcaggtt tgtccaaccg ccacctgtcg cgctgggcga agcgggtgtt
     4801 gtacgctttt ccgaaagcgt tcagccacga aggcggtttg gtcggcaatc ccgtccgcgc
     4861 cgatattagc aacctgcccg tgcctgccga acgcttccaa gggcgtgaag gccgtctgaa
     4921 aattttggtg gtcggcggca gtttgggcgc ggacgttttg aacaaaaccg taccgcaggc
     4981 attggctttg ctgcccgaca atgcgcgtcc gcagatgtac caccaatcgg gacggggcaa
     5041 gctgggcagc ttgcaggcgg attacgacgc gctgggcgtg aaagccgaat gtgtggaatt
     5101 tattaccgac atggtgtccg cctaccgcga tgccgatttg gtgatttgcc gtgccggcgc
     5161 gctgacgatt gccgagttga cggcggcggg attgggtgcg ttgttagtgc cgtatcctca
     5221 cgccgttgat gaccatcaaa ccgccaacgc gcgttttatg gtgcaggcgg aggcgggatt
     5281 gctgttgccg caaacccagt tgacggcgga aaaactcgcc gaaatcctcg gcagcctgaa
     5341 ccgcgaaaaa tgtctcaaat gggcagaaaa cgcccgtact ttggcattgc cgcacagcgc
     5401 ggacgacgtt gccgaagccg cgattgcgtg tgcggcgtaa actgccgaac catgccgtct
     5461 gaaaagccgt tcagacagca tggatgtttt ttatttcaat ccgctatata tttgtcagaa
     5521 aactatggcg cgcaaacggt cagcccttta aaataacgcc tttatgcatc gaaaatccac
     5581 cggaacgcaa cattatgatg aaaaatcgag tgaccaacat ccattttgtc ggtatcggcg
     5641 gcgtcggcat gagcggcatc gccgaagtct tgcacaattt gggctttaaa gtttccggtt
     5701 cggatcaggc gcgaaatgcc gctaccgagc atttgggcag cttgggcatt caagtttatc
     5761 ccggccatac cgcagaacac gttaacggtg cggatgtcgt cgttacctct accgccgtca
     5821 aaaaagaaaa tcccgaagtt gtcgctgcgt tggagcagca aattcccgtt attccgcgcg
     5881 ccctgatgtt ggcggagttg atgcgcttcc gtgacggcat cgccattgcc ggcacgcacg
     5941 gcaaaaccac gaccaccagc ctgaccgcct ccatcctcgg cgcggcagga cttgacccga
     6001 ctttcgttat cggcggcaaa ctcaacgccg caggcaccaa cgcccgcttg ggcaaaggcg
     6061 aatacatcgt tgccgaagcc gacgagtcgg atgcatcctt tctgcacctg acaccgatta
     6121 tgtccgtcgt taccaatatc gacgaagacc atatggatac ctacgggcac agcgtcgaaa
     6181 aactgcatca ggcgtttatc gatttcatcc accgtatgcc cttctacggc aaagcctttt
     6241 tgtgtattga cagcgaacac gtccgcgcga ttttgcccaa agtgagcaaa ccttatgcta
     6301 cttacggttt ggacgatacc gccgacatct acgccaccga catcgaaaac gtcggcgcgc
     6361 aaatgaaatt caccgtccat gttcaaatga aaggacatga gcaggggtcg tttgaagtcg
     6421 tgctgaatat gcccggcaga cacaacgtgc tgaacgcatt ggcagccatc ggcgtggcgc
     6481 tggaagtcgg cgcatcggtt gaagcgatcc aaaaaggctt gctcggcttt gaaggcgtcg
     6541 gccgccgctt ccaaaaatac ggcgacatca agttgccaaa cggcgggacc gcgctcttgg
     6601 tggacgacta cggacaccac cccgtcgaaa tggcggcgac ccttgccgcc gcacgcggcg
     6661 cgtatccgga aaaacgtttg gtactcgcct tccagccgca ccgctatacc cgcacgcgcg
     6721 atttgtttga agactttacc aaagtcctca ataccgttga cgcgctggtg ctgaccgaag
     6781 tttatgccgc cggtgaagag ccgattgccg ccgccgattc ccgcgctctt gcccgcgcca
     6841 tccgcgtgtt gggcaaactc gagccgattt actgcgaaaa cgttgccgat ctgcccgaaa
     6901 tgctgttgaa cgttttgcag gacggcgaca tcgtgttgaa tatgggcgcg ggaagcatca
     6961 accgcgtccc cgccgcgctg ctggaattgt cgaaacagat ttgaggcaca cccgcctgac
     7021 agacggaaca tcatataaag gtcgtctgaa accgcaaatc aggtttcaga cgacctctgg
     7081 caacaagcat aaagcaatca ggaaagaaca aaaacaatgc agaattttgg caaagtggcc
     7141 gtattgatgg gcggtttttc cagcgaacga gaaatctcgc tggacagcgg caccgccatt
     7201 ttgaatgctt taaaaagcaa aggcatagac gcatacgcct tcgatcctaa agaaacccca
     7261 ttgtctgaat tgaaggcaca aggttttcag acggcattca acatccttca cggtacttac
     7321 ggcgaagacg gggcggttca gggtgcattg gaactgttgg gcattcccta taccggcagc
     7381 ggtgtcgccg catccgccat cggcatggac aaataccgct gcaaactgat ttggcaggca
     7441 ttgggattgc ccgttcccga gttcgccgtc ctgcacgacg acactgattt cgatgccgtc
     7501 gaagaaaaat tgggcctgcc gatgtttgtg aaaccggcgg ccgaaggcag cagcgtaggc
     7561 gtggtaaaag tcaaagaaaa aggccgtctg aaaagcgttt acgaagaatt gaaacacctt
     7621 cagggcgaaa tcattgccga acgttttatc ggcggcggcg aatattcctg ccccgtcctg
     7681 aacggcaaag ggctgcccgg catacacatc attcccgcaa ccgagtttta tgactacgaa
     7741 gccaagtaca accgcaacga caccatttat caatgtcctt cggaagatct gaccgaagcc
     7801 gaagaaagcc tgatgcgcga actggcggtt cgcggcgcgc aggcaatcgg tgcggaaggc
     7861 tgcgtgcgcg tcgatttcct caaagatacc gacggcaaac tctatctgtt ggaaatcaac
     7921 accctgcccg gtatgaccgg ccatagttta gtaccgaaat ccgctgccgt tatgggcgtg
     7981 ggttttgccg atttatgtat tgaaattttg aagaccgcac atgtgggata atgccgaagc
     8041 gatggaacgg ctgacgcgct ggctgcttgt catgatggcg atgctgcttg ctgcgtccgg
     8101 gctggtttgg ttttacaatt cgaatcatct gcccgtcaag caggtgtcgc tgaagggcaa
     8161 cctggtttat tccgataaga aggcattggg cagtttggcg aaagaataca tccatgggaa
     8221 tattttgagg acggacatca atggcgcaca ggaggcctac cgccggtatc cgtggattgc
     8281 gtcggtcatg gtgcgccgcc gttttcccga cacggttgag gtcgtcctga ccgagcgcaa
     8341 gccggtcgcg cgttggggcg accatgcctt ggtggacggc gaaggcaatg tttttgaagc
     8401 ccgcttggac agacccggaa tgccggtatt cagaggcgcg gaaggaacgt ctgccgaaat
     8461 gctccgccgt tatgacgaat tttcgactgt tttggcaaaa cagggtttgg gcatcaaaga
     8521 gatgacctat acggcacgtt cggcgtggat tgtcgttttg gacaacggca tcaccgtcag
     8581 gctcggacgg gaaaacgaga tgaaacgcct ccggcttttt accgaagcgt ggcagcatct
     8641 gttgcgtaaa aataaaaatc ggttatccta tgtggatatg aggtataagg acggattttc
     8701 agtccgctat gcttccgacg gtttacccga aaaagaatcc gaagaatagt gggaacaggt
     8761 atcggacaga ttacggccgt gccgtctgaa atggtgcgac gcaaatttca atcagtttta
     8821 agagcagacg aacaatggaa cagcagcaaa gatacatcag cgtactggat atcggtacgt
     8881 ctaaagtcct cgcactgatc ggggaagttc aagatgacga caaaatcaac atcgtcggtt
     8941 tggggcaggc tccttcacgg ggcttgcgcg cgggcatggt aaccaatatc gatgccaccg
     9001 tccaagccat caggcaggcg gtcaatgatg ccgagctgat ggcggatacc aaaattaccc
     9061 acgttaccac aggtatcgca ggcaaccaca tccgcagtct caattcgcaa ggtgtggtta
     9121 aaattaaaga cggggaagtc acgcaggcag acatcgaccg cgccattgaa acggcaaagg
     9181 caatcaatat cccgcccgat caaaaaattc tcgatgccgt ggttcaagac tacattatcg
     9241 acacccaact tggcgtgagg gagcccatcg gtatgagcgg tgtgcgtctg gatacgcggg
     9301 tgcacatcat taccggtgca agtacggcag tgcagaatgt ccaaaaatgt atcgagcggt
     9361 gcggcttgaa aagcgatcaa atcatgcttc agccgttggc aagcgggcag gcggtgctga
     9421 ctgaagatga aaaagacctc ggcgtatgcg tcatcgacat tggtggcgga acgaccgata
     9481 ttgccgttta tatgaacggt gccatccgcc atacgtccgt cattccggcc ggtggtaatc
     9541 tgattaccaa agatttgtcc aaatcgttga gaacacctct cgatgccgcc gagtacatta
     9601 aaatccatta tggcgtggca tcatgcgata cggaaggctt gggtgagatg attgaagttc
     9661 cgggcgtggg tgaccggaca tcgcgtcagg tttccagtaa ggttctggca gcaatcatca
     9721 gtgcacggat tcaggagatt tttggcgtag tgctgggcga gctgcaaaaa tcgggtttcc
     9781 ccaaagaagt gctgaatgcg ggtatcgttc tgaccggcgg tgtgtccatg atgaccggga
     9841 ttgtggaatt tgccgaaaaa atcttcgatt tgcctgtacg caccggtgca ccccaagaaa
     9901 tgggcggttt gtccgaccgc gtccgcacac cgcgtttttc taccgctatc gggctgcttc
     9961 atgcagcatg caagctggaa ggaaacttgc cgcagccgga aaacggtgca gtgcaagaga
    10021 gggaaggggg cggcggtttg ttggcaagat tgaaacggtg gattgaaaac agcttctgaa
    10081 caggtggatt gccgtttgac aggtgagaag tattttgcca gcagcaagat acttcttata
    10141 taatgaataa taatttattt aaaccgtcct ctgaatgggg cgagcaggag tttttgaatg
    10201 gaatttgttt acgacgtggc agaatcggca gtcagccctg cggtgattaa agtaatcggc
    10261 ttgggcggcg gcggttgcaa tgcaatcaat aacatggttg ccaacaatgt gcgcggtgtg
    10321 gagtttatca gtgccaatac ggatgcgcag tctctggcaa aaaaccatgc ggcgaagaga
    10381 atccagttgg gtacgaatct gacacgcggt ttgggcgcgg gcgcgaatcc cgatatcggc
    10441 cgtgcggcag cccaggaaga ccgggaagcc attgaagaag ccattcgcgg tgcgaatatg
    10501 ctgtttatca cgaccggtat gggcggcggt accggtaccg gttccgcgcc ggttgttgct
    10561 gagattgcca agtctttggg cattctgacc gttgccgtgg ttacccgacc gttcgcatat
    10621 gaaggtaagc gcgtccatgt cgcacaggca gggttggaac agttgaaaga acacgtcgat
    10681 tcgctgatta tcatcccgaa cgacaaactg atgactgcat tgggtgaaga cgtaacgatg
    10741 cgcgaagcct tccgtgccgc cgacaatgta ttgcgcgatg cggtcgcagg catttccgaa
    10801 gtggtaactt gcccgagcga aatcatcaac ctcgactttg ccgacgtgaa aaccgtgatg
    10861 agcaaccgcg gtatcgctat gatgggttcg ggttatgccc aaggtatcga ccgtgcgcgt
    10921 atggcgaccg accaggccat ttccagtccg ctgctggacg atgtaacctt ggacggagcg
    10981 cgcggtgtgc tggtcaatat tacgactgct ccgggttgct tgaaaatgtc cgagttgtcc
    11041 gaagtcatga aaatcgtcaa ccaaagcgcg catcccgatt tggaatgtaa attcggtgcg
    11101 gctgaagacg agaccatgag cgaagatgcc atccggatta ccattatcgc taccggtctg
    11161 aaagaaaaag gcgcggtcga ttttgttccg gcaagggagg tagaagcggt tgccccgtcc
    11221 aaacaggagc aaagccataa tgtcgaaggt atgatccgca ccaatcgcgg tatccgcacg
    11281 atgaacctta ccgctgcgga tttcgacaat cagtccgtac ttgacgactt tgaaatccct
    11341 gcgattttgc gtcgtcaaca caattcagac aaataatgtg ctgtttgccc gtaaacctgc
    11401 tgcctcccga atcggtttgt ccggtttggg aggtatgttt ttcaagatgt tgcaatttcg
    11461 tacggtttgc ggtcggcgga ttcagatttt tccacttgat acagactttc agatatggac
    11521 acttcaaaac aaacactgtt ggacgggatt tttaagctga aggcaaacgg tacgacggtg
    11581 cgtaccgagt tgatggcggg tttgacaact tttttgacga tgtgctacat cgttatcgtc
    11641 aaccctctga ttttgggcga gaccggcatg gatatggggg cggtattcgt cgctacctgt
    11701 atcgcgtctg ccatcggctg ttttgttatg ggttttgtcg gcaactatcc gattgcactc
    11761 gcaccgggga tggggctgaa tgcctatttc acctttgccg tcgttaaggg tatgggcgtg
    11821 ccttggcagg ttgcgttggg tgcggtgttc atctccggtc tgatttttat cctgttcagc
    11881 ttttttaaag tcagggaaat gctggtcaac gcactgccta tgggtttgaa aatgtcgatt
    11941 gctgccggta tcggtttgtt tttggcactg atttccctga aaggcgcagg cattatcgtt
    12001 gccaatccgg caaccttggt cggtttgggc gatattcatc agccgtccgc gttgttggca
    12061 ctgttcggtt ttgctatggt ggtcgtattg ggacatttcc gcgttcaagg cgcaatcatc
    12121 atcaccatct tgaccattac cgtcatagcc agcctgatgg gtttgaatga atttcacggc
    12181 atcatcggcg aagtaccgag cattgcgccg acttttatgc agatggattt tgaaggcctg
    12241 tttaccgtca gcatggtcag tgtgattttc gtcttcttct tggtcgatct atttgacagt
    12301 accggaacgc tggtcggcat atcccaccgt gccgggctgc tggtggacgg taagctgccc
    12361 cgcctgaaac gcgcactgct tgcagactct accgccattg tggcaggtgc ggctttgggt
    12421 acttcttcca ccacgcctta tgtggaaagc gcggcgggcg tatcggcagg cggacggacc
    12481 ggcctgacgg cggttaccgt cggcgtattg atgctcgcct gcctgatgtt ttcacctttg
    12541 gcgaaaagtg ttcccgcttt tgccaccgcg cccgccctgc tttatgtcgg cacacaaatg
    12601 ctccgcagtg cccgggatat tgattgggac gatatgacgg aagccgcacc tgcgttcctg
    12661 accattgttt tcatgccgtt tacttattcg attgcagacg gcatcgcttt cggcttcatc
    12721 agttatgccg tggttaaact tttatgccgc cgcaccaaag acgttccgcc tatggtatgg
    12781 attgttgccg tattgtgggc actgaaattc tggtatttgg gctgattgat tcgatattaa
    12841 aaatgccgtc tgaaaggttt tcagacggca ttttgtttgc ca
//
LOCUS       DAXHSJ010000046        12300 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000046 DAXHSJ010000000
VERSION     DAXHSJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12300
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.046"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..203)
                     /locus_tag="ACFM8U_000920"
     CDS             complement(<1..203)
                     /locus_tag="ACFM8U_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein GemA/Gp16 family protein"
                     /protein_id="HFC6334179.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKP"
     gene            complement(375..557)
                     /locus_tag="ACFM8U_000921"
     CDS             complement(375..557)
                     /locus_tag="ACFM8U_000921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334180.1"
                     /translation="MIAISFLIAAVLITTLWRDKDSVAIEIVKIGGAVALGYFAGINR
                     GKAQTLEKQRREKDSD"
     gene            complement(563..790)
                     /locus_tag="ACFM8U_000922"
     CDS             complement(563..790)
                     /locus_tag="ACFM8U_000922"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334181.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEILKRYTNAV"
     gene            complement(787..978)
                     /locus_tag="ACFM8U_000923"
     CDS             complement(787..978)
                     /locus_tag="ACFM8U_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334182.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            complement(1022..1264)
                     /locus_tag="ACFM8U_000924"
     CDS             complement(1022..1264)
                     /locus_tag="ACFM8U_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334183.1"
                     /translation="MDTLKVNPFPNKIKEKCRLKTARMLFQTAFFVVGHQCPAICAAL
                     RLSRYGILVCIFRRNLPYIVLKYYTVVLYGFDFLEK"
     gene            1501..1668
                     /locus_tag="ACFM8U_000925"
     CDS             1501..1668
                     /locus_tag="ACFM8U_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334184.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            1941..2126
                     /locus_tag="ACFM8U_000926"
     CDS             1941..2126
                     /locus_tag="ACFM8U_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334185.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            2327..2527
                     /locus_tag="ACFM8U_000927"
     CDS             2327..2527
                     /locus_tag="ACFM8U_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334186.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            2677..3267
                     /locus_tag="ACFM8U_000928"
     CDS             2677..3267
                     /locus_tag="ACFM8U_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFC6334187.1"
                     /translation="MNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSKWIQRRI
                     KEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERNDKGRQAR
                     RYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIGKLLDMN
                     PGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            3271..3486
                     /locus_tag="ACFM8U_000929"
     CDS             3271..3486
                     /locus_tag="ACFM8U_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334188.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(3647..4165)
                     /locus_tag="ACFM8U_000930"
     CDS             complement(3647..4165)
                     /locus_tag="ACFM8U_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFC6334189.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAQAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(4275..4442)
                     /locus_tag="ACFM8U_000931"
     CDS             complement(4275..4442)
                     /locus_tag="ACFM8U_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334190.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            complement(4435..4608)
                     /locus_tag="ACFM8U_000932"
     CDS             complement(4435..4608)
                     /locus_tag="ACFM8U_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334191.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            complement(4717..4983)
                     /locus_tag="ACFM8U_000933"
     CDS             complement(4717..4983)
                     /locus_tag="ACFM8U_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334192.1"
                     /translation="MQVLKKVDWKMFVARSFWRSVPVGLTVGVWCFVAGMALHSCAKE
                     PEPVAKEPTKIEKMERQADLEVKVWEEQYESMSVEEKMKGIVYE"
     gene            complement(5073..5987)
                     /locus_tag="ACFM8U_000934"
     CDS             complement(5073..5987)
                     /locus_tag="ACFM8U_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6334193.1"
                     /translation="MKQINQALQQKLAEFKEKSGMSQTQLARGIDTSPASVSMYLNGT
                     YAEKGGNYETIEPKIEAFLEMQDSKAKREELVLGFVSTKTTRRIAEAMRDAHEGGEIV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSLPLNLGESLPDDELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKVQAGIVKKYSSLIIR"
     gene            6058..6135
                     /locus_tag="ACFM8U_000935"
                     /pseudo
     CDS             6058..6135
                     /locus_tag="ACFM8U_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(6202..8190)
                     /locus_tag="ACFM8U_000936"
     CDS             complement(6202..8190)
                     /locus_tag="ACFM8U_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFC6334194.1"
                     /translation="MGITIGELLKLNIQGLPTTARGLRERAKTQNWPYIEEVGKARGG
                     RLKKYLIASLPAEIRAAIMKRQSDELTEKMPKTLPQVRPGTAMSAQALAEAAKLLNEK
                     QRSVADARCAVVAAVLGIKYQYGCSAKAAVAQFLGLLAESKLDAVTLGNLEKANDRSR
                     SAKVGERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSA
                     PKLAHSYRRFVQWAEAENMPVNDVPSLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPY
                     VKRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAE
                     SCVAVSDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNP
                     QGRGIIERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQ
                     QKYLKKLPGWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYSPKAYREMRLEQDGIAP
                     DMLSAEELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASAVN
                     VFDMDGKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALE
                     QAAVWDELGHLGGNVIEAEYAVLPRTDTDDFVLFEADR"
     gene            complement(8201..8389)
                     /locus_tag="ACFM8U_000937"
     CDS             complement(8201..8389)
                     /locus_tag="ACFM8U_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6334195.1"
                     /translation="MGIKTVNEVREWLKDNNLTVAQWARENGHNSVDVTRVLTGKSKC
                     RYGQGRAIAIKLGLRIDE"
     gene            8509..8871
                     /locus_tag="ACFM8U_000938"
     CDS             8509..8871
                     /locus_tag="ACFM8U_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6334196.1"
                     /translation="MDNSFELSSKLLGERLKKIRTDNGYSREQLSELFGISRASIQNY
                     ENGERSPNADYLVQFYKHFGINLHWLLTGNKAAKFQDFIDSVSSPREEVLLHLARQMD
                     SRTLNHLIDFLMDVYAKK"
     gene            9048..9587
                     /locus_tag="ACFM8U_000939"
     CDS             9048..9587
                     /locus_tag="ACFM8U_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6334197.1"
                     /translation="MLTGKGLPYLDKARLENAGAFSVSRNSDGITDTMGNPVDLSEFV
                     FVPRYNVAAAAGYGAPVFGEEPLFCLAFRKYWIDNYVTKQPEKLSVITVKGDSMEGVL
                     NHGDSILINHAKTDPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAPFEIDFSN
                     AQDDIAIIGRVEWYGRSID"
     gene            9796..10386
                     /locus_tag="ACFM8U_000940"
                     /pseudo
     CDS             9796..10386
                     /locus_tag="ACFM8U_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            10491..11159
                     /gene="hda"
                     /locus_tag="ACFM8U_000941"
     CDS             10491..11159
                     /gene="hda"
                     /locus_tag="ACFM8U_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC6334198.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            11156..11824
                     /locus_tag="ACFM8U_000942"
     CDS             11156..11824
                     /locus_tag="ACFM8U_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6334199.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT         3217 a         3314 c         2593 g         3176 t
ORIGIN      
        1 ggtttaccgt gtgggttacc ttttgcttta ggcttaaatc cgtgcgaccg catatcagcg
       61 acaacagact caagttcgga aacatccata tccgcacacg accgcttgcc cgtcacgcgc
      121 tccaacaccg cgcgataggt accgtcgtcc aagcccagct ccttttgggc aattttaatt
      181 tttgcaatca acgcccggcg cattatctct ccaatacaac atatagtata aattagcgca
      241 tattatacca ataaaatata atatatagta ttaagccgat gttttttgcg aaacggacag
      301 acatgaaaaa ggccgtctga aaacgagctt tgcgagtttc gccaaaacgt ttcagacgac
      361 ctttttacta caatctaatc actgtctttt tcgcgccgtt gcttttccaa ggtttgcgct
      421 ttacctctgt tgattcctgc aaaatatccc aaagctaccg ccccgccgat tttgacaatt
      481 tcaatagcga cgctgtcctt atcgcgccat aacgtagtaa tcagcacggc ggcaatcaga
      541 aaagaaatag caatcacggc attcagacgg cgtttgtgta cctctttaaa atctcaccat
      601 gttgctgttc accctgtttt tgcgcttcga tggtagccag tgcaatgcgt tcgtgagatt
      661 ggatttcgtc cttacggctt tccaactcct tctgtttgac tgcaatatct tgtttttgaa
      721 gttctaaaaa ctcctgaagc attttctgct gctcatcggc cggcagattc tcggattttt
      781 ccccgctcat aaatcaaccg ccactaaggt tttgtaacgg actttgaatt tgccgtaacc
      841 ccgagcccct attttcggcg gcacaaattc cacggacgca atattgcccg tcttacggtc
      901 tttacggact tcttccaaat aatcggcagc attgagcagc cgttcgcgga ctgccgtata
      961 ttctttgacg gtattcattt ttcaacctcg cccaaatagt gcaaatatta gcatatggga
     1021 tttatttttc aagaaaatca aatccatata aaacaaccgt ataatatttt aatactatat
     1081 atggtaaatt tcttctaaaa atacacacta aaataccata tcttgataac cgcaacgccg
     1141 cacaaatggc agggcattga tgcccgacaa caaaaaatgc cgtctgaaac agcattcgag
     1201 ctgttttcag acggcatttt tctttgattt tgttggggaa agggttgact tttaacgtgt
     1261 ccacgtttat aattcaattc atcggcgtag tgagggccga tacgggaaaa gccccaccga
     1321 agcggggctc aactgggaga aagaagatga ataaacgcac tattcaaatt ctcatcttgg
     1381 tcgcagtttt catgttggca agtgctaatg cctactaaag actgaccagc tgggggcggt
     1441 cgccgctgtc cccagtccca atcttacaaa atacggttta aaaaatcaag gagtttgcct
     1501 atggttgatg aaaaactggc agaaaaccga aagcgttacg agcagaaacg ggtgattaag
     1561 aaagtctcgt tcaatgccga aacggaaaaa gaacttttgg aatatgccca aaatatcgat
     1621 ttttcccaat gggttaaatc aatcatcaaa gaaaaaatca aaaaatagct tgactaaatt
     1681 ctagaaatac tagataatac gcctatctga taaagcaaag ccccgaagcg gcaacttcgg
     1741 gggcttatcg gatagaaagg aggcttgtgg aaatgacaaa actggcgaaa gttgtttttt
     1801 tcatcattct gcttttaagc tgtagtccgg cttactaagc aagttgatta aacacaaggg
     1861 gtggcagaac accgcgcccc tttcgccaac ctttctgcaa gccattttac tacaacgatt
     1921 ttaaaaatca aggattgcct atgcctgatg aaaaaacgct aaaagcaaaa caagctgaat
     1981 atcagcgaaa gtatgaccaa aagcggctga tgaaaacagt ttctttccat ttggaaagag
     2041 agaaagagtt ggtggatttt atgcaaaaag aaattcccga cttttcaaac tgggttaaat
     2101 cagtcatgaa ggaaaaaatg aaataagaaa attccgccga tgggaaaaca tcggcggaac
     2161 aggtggaaag cagagcagca actctcttta tgtagggcat gaaaaaaggc cgtctgaaac
     2221 aggttttaaa ccccatttca gacggctttt tgttgtaata catcttgatt aaaatggttt
     2281 ttagtattac cataatcgtg ttttaaaacc tatttaaagg aagattatgg actcgattaa
     2341 aaaggctcaa gaaaaatacg cactcaagcg agtagtgaaa acagtatcgt tcaattccga
     2401 aaaagaactt gatttaatta aattttctga aaatttagat ttttcaaact gggtaaaatc
     2461 gaaaattaga ggagatatgg aatcggaaaa attcaaaaaa aaagcaaaaa agaatattga
     2521 caaatagaat taccataatt taaaatttcc atatcttgtg caacacaaga tatggaaatc
     2581 cccgataagt gacagcttat cggggatagt ggaaaagcaa cgttagagcg ttcttttcct
     2641 tgtcatctca accttgaaat gaaagataat tttaccatga atacccaact catcccaact
     2701 gtttcaggtc aattggacaa tcaaacacaa gcactggttg atgctcatga cctgcataag
     2761 ttcttaggtg tggaaacacc attttctaaa tggattcagc gtcgcattaa agaatacgga
     2821 tttacacaag cccttgattt tatcggcgtg gacaaaattg tccgcaccga agcaggtttc
     2881 tttggacagc gtgataaaac tgttcagggg tattatttat ccctcgacat ggcaaaagag
     2941 ctgtgcatgg tggagcgcaa cgacaaagga cgccaagccc gccgatattt tatcgaaatg
     3001 gagaaacagg caaaagccct gcctgatgcc gtgctatacc gcatcgatgc attggaagac
     3061 gcctatttcc aagccgcgcc cgaaatgctc gcgctgctgc gctaccgcag tatgggcttg
     3121 aacctgaccg aaataggcaa actcttggac atgaaccccg gcgcggtttc ctaccgtctc
     3181 aaaaaactca acgatttggg tttcttggaa tatgtcccca agcccaaaat ggtggtgcaa
     3241 caacagtctt tgggattgga gggttaagcc atgaatacca cctataccct gactttcgac
     3301 caagacagcc tcaaagccct gaatctgctg gtgttcacac tcaaccatct gaaagttttg
     3361 gagatggaca tggaaggaat cgaagacggt ttgtctgccg tgattgaaac cgcctccgag
     3421 aaagccgaca agctgtccgc cgctttctac aacgccgtgt acgagcagga agagaaagca
     3481 gcctgaatct agggcatgaa aaaaggccgt ctgaaacagg ttttaaaccc cgtttcagac
     3541 gacctttaaa tcatgggtta aagcctattt gaaccggcta ggcaagctcc tgctccgtag
     3601 gctcaatcac gtttcaacac actgaattga aatcaggctg ttgattttat agaatttgtt
     3661 cgctcggctc aatcacaaaa tcctccacgc cgctcacaat cttaatcccc ggcacttggc
     3721 cgtctgaaaa acgctctttt tgattcagga tggcgtcttt gtcgatttcc tttttagtgc
     3781 ggacaaactc ggcaaaggcg gatttttccg agagccacgc caagacggcg gccacgcccg
     3841 ttaccttgac ggatggcgga cggatgcgcc atttaatcag gccggtagta aagtccacgg
     3901 ttttggtttt accgttttcc gtcagctcgt ccttatgtgc ctcgcagtat gcggccacac
     3961 gttcggtcag gctcatgatt tcggcacaca tcggcgcggc ttgggcggca tattcttctt
     4021 cgatggccgc ttttttgtct ccggcttcgg tttccaggcg tttgacttcg cgctgcaagt
     4081 cgccgatttg gcggataaat gcagtaactt ccgttttgtc ttgtgccgct tcgatggcgg
     4141 gctgtttgat tcgggtttta gccattttct cttcctttca ggttgggttt aacatttcgg
     4201 cttactcaag ccttgttcgc gttcgtgctt ggcaagtgct tcaactttcg cgcggtttct
     4261 caaaaactta aacatcacac ttcctcccat aaagttatcg cctgagccaa agtttccgcc
     4321 tcggcagtct tccacatccc gtccggcgca cgcgctgcaa tcacaaagcc ttcgccgtcc
     4381 ttcttcatga ccatcagctc gccacggtct tcgagccatt cgattaaatc tttttcattc
     4441 atctttgttt ccttccgttt tcagacgacc tttgccgtcc ttatcttcaa actgcgcctg
     4501 atattcctcg atttcgcgct cccggctttt ttgcgccatt cgcgccgtcg cacgcctgcg
     4561 gtgttgtccc caagcctgcc aatccgtatt acgtcgtccg aaactcattt cacacatcct
     4621 ttcacaatcg ccttatcgcc atatttcgca tggatttcct ttaccgcccg atccaaagcc
     4681 tcttttttcg ccgcagggct caataaaatt ggtttatcac tcataaacaa tccctttcat
     4741 tttttcctcg acgctcattg actcgtattg ctcttcccat accttgacct ccaaatctgc
     4801 ctgtctttcc atcttctcga tcttcgtcgg ctctttcgca accggttcgg gttctttcgc
     4861 gcaggaatgc aacgccattc ccgccacaaa acaccacacg cccaccgtca aaccaaccgg
     4921 cacactccgc caaaaagagc gtgccacaaa catcttccaa tcaactttct tcaaaacttg
     4981 cattttgcgt tttcctttag aaaacagtaa cttattaaaa tcatagggta aaaaatatat
     5041 agccctgtca gagacttacc gtttcagacg gcctatcgga taatcaggga gctgtatttc
     5101 ttaacgatac cggcctgcac cttgataccg ttcttgttcg ccgtgcgtac cgcgccgcgc
     5161 atcaatttgc tcatccggcg cgtattgccg ttactatgtt taaccagttc caagagcgtt
     5221 tcttcgtccg catcaggcaa agccgctttc gcaatctcaa agagttcgtc atccggcaaa
     5281 gattcgccca aattcagcgg aagcgacacg cggctgtaaa gctgtaccag ctcgccgtgc
     5341 ttgccgcgca aattcgcaac cagtcggggc ataccgctca acaccaaacc gcagccagtc
     5401 tcatcgtgca gtcggcggat aatctcaagg gcgcgtaacg gcaggttttc cgcttcatcg
     5461 accacaatca gacggcccga atcacgcagg cggtcagata cagactcaaa caaatcattc
     5521 aggctgccca tcgccgacac cttcgccgcc gtcgccaact tgcgcatcag gaccaaagcc
     5581 gtaaagctcg gattagcctc aatcaagatg gccgcagggt ttttctcgca gtagtttttg
     5641 accgcctgag tcttgcccaa accggcctga ccgtagatca ccactatttc gccgccttcg
     5701 tgcgcatcgc gcatcgcttc cgcgattcgg cgtgtcgtct tagtcgatac aaaacccaac
     5761 accagctctt cgcgtttcgc tttactgtcc tgcatctcca aaaacgcctc gattttcggc
     5821 tcgatggttt cataattgcc gcctttttcc gcataagtgc cattcagata catactgacg
     5881 gatgccggcg aagtatcgat accgcgtgcc agttgggttt ggctcatacc cgatttttct
     5941 ttaaattcag ccagtttttg ttgcaatgct tgattgattt gtttcatttt taatatcctt
     6001 gagttttaaa caacctttaa aggccgtctg aaatgaaaga gtttcccgat ttagaaattt
     6061 gagcctgatt ccgttcagcg ggaacacctt gaatctttac ttgaaatcgt tgaatccgcc
     6121 caagggcccg cttgagtttc aatttttcaa caagctcggt cgaaaacgcc gcatttttct
     6181 gtttgtccat catgttttcc tttatctatc cgcctcaaat agcacaaaat cgtccgtgtc
     6241 cgttctcggc aataccgcat actccgcctc gatgacgttt ccgcccaaat gtcccagctc
     6301 gtcccaaacc gcagcctgtt ccagagccgg attgacttcc gcgtttgcga gcttgattgc
     6361 attttccgcc cgcttgattt tgccttttcg gcgtttttcc gccagttggt cgatacgcgc
     6421 cgtcgggaaa gcctcgcggg tattgccgtt ggcctgcgcc ttagtaataa acttgccgtc
     6481 catatcaaac acattgaccg ccgacgcatc gctcaaatcg tagctgaccc gtacctcgtc
     6541 cttgtgatac tccgccagct cagttgagaa ataagagttg ttgaacaaat ccagccaacc
     6601 gcgctgtacc tttcgcacct cttgcggcat aaacatcgtc gccagctctt ccgccgacaa
     6661 catatccggc gcgataccgt cctgttccag cctcatttcc cgataagcct tcggcgaata
     6721 atgcccgccg tccggatgtc ggggcagctc gccgtgcggg cggttgttgt attcgtcgat
     6781 acacttgacc acatccgcga taaaacgcga ccagcccggc agttttttca aatatttctg
     6841 ttgttcctcc gtcaaatcct tgcctttttc caaagcgtta aacgcacttt ccatcttgcg
     6901 gtacatcagg ttcttggtac tgctgtccat ccccgcgccc gcaaacgtct catactgtcg
     6961 cgccatctca atcagattgt ctttccacca tcgctcgatg atgcctcggc cttgcgggtt
     7021 gcccgcgata cccgtttcat gccggatacc caatcgggac gtaatacccg tgatttcatg
     7081 gtctatcgtc ttgccggttt ggccgccacc gttatccgag tagtagataa tcggcaaacc
     7141 aaagtgcttg accccgatac gcagagcgtc cgataccgcc acacaacttt cagccaacga
     7201 gaccgaaaac ccgaccacaa accgcgtaca accatcaata atcaccgtca cttccggctt
     7261 aaacggcctg ccatgtaccg gatgcgccac ctttgcctta aagctatggc cgtcgccgat
     7321 ccaaacatcg ttcggcttca aagcccccca atcacgtttc acataaggca gcagcgattt
     7381 ataagccgcc cccgttttcc tgccgcgctc ctgcataatc agcgggagct tgtcccaaac
     7441 gcgccgcacc atactcaagc taggcacatc attgaccggc atattttccg cttccgccca
     7501 ctgcacaaac cggcggtagc tgtgtgccag tttcggcgcg gacgggatat tgtgaaactg
     7561 cataaacatc ggcaaccaac cgtagctctc aatcggcttg accgccttcg tcgtcttcgg
     7621 agccaaagca accaaccgct ccgtcgcgtt ttccgctttc aaataagcag aaatccagcc
     7681 gtctaaagta cgttcgccaa ccttcgccga ccggctgcgg tcattggcct tttccaagtt
     7741 cccaagcgtg accgcgtcca atttactttc tgccagcaag cccaaaaact gagccaccgc
     7801 agcctttgca gagcaaccgt attggtattt aatccccaat accgccgcca ccaccgcaca
     7861 tcgcgcatcc gccaccgacc gttgtttctc gttcaacagc ttggccgctt cagccagtgc
     7921 ctgagccgac atcgccgtcc ccggtctgac ttggggcaag gttttcggca tcttctccgt
     7981 cagctcgtcc gactgccgtt tcatgatggc tgctcggatt tcggcaggga gggaagcgat
     8041 taagtatttt ttcagacggc ctccacgcgc tttgccaacc tcttcgatgt acggccagtt
     8101 ctgggttttt gctctctccc ttaatcctct tgctgttgta ggaagccctt gaatattaag
     8161 tttaagcagc tcaccaatcg ttatccccat cttcgtttcc tcactcgtca attcttaacc
     8221 ccagcttaat cgcgatagcg cgcccctgac cgtatcgaca ctttgatttc cccgtcaaca
     8281 cacgagtaac atctacggag ttgtgtccgt tttctctcgc ccattgcgca actgttaaat
     8341 tattgtcttt taaccattct ctaacttcat ttactgtttt aattcccatt tttttacctt
     8401 tcttggtttg ttcaaatcct gtataattac tacaaaacta gctgttttgc tacatttgta
     8461 gcaatattat tagctacaaa tgtagctgtc aagaaaaaag gaacttttat ggataattct
     8521 tttgaattaa gctctaagtt attgggtgag cgactaaaaa aaatcagaac agataatgga
     8581 tactcaagag agcagttgag tgagttattt ggcatcagta gggcaagtat tcaaaattat
     8641 gagaatgggg aaagaagtcc gaatgccgat tacttagttc agttttataa gcactttgga
     8701 attaatttgc attggctgct aactggaaat aaggctgcca aattccaaga tttcattgat
     8761 tctgtttctt ctccgagaga agaagtacta ctacacttgg ctcgccaaat ggatagtcgc
     8821 accttgaatc acttgattga ctttctaatg gatgtttatg ctaaaaaata aacagcacta
     8881 aggaaaactc agcttttaag ttccttcctt agtgaacagt cgcctaaata gagaaatttt
     8941 ttttaaattt tgattttgaa atgaaaaata caaaggaagg cttctaaacc tcttcctttg
     9001 tattttcctt tgtcttgcaa gaatctacag gctgagattt aaactggctg ctgaccggca
     9061 aaggcttacc gtatcttgat aaagccaggc tggaaaacgc tggagcattc tctgtaagca
     9121 gaaattctga tggaattact gacactatgg gcaaccctgt cgacttgtca gagtttgtat
     9181 ttgtgccgcg ctacaacgta gccgcagcag cagggtatgg cgcaccggtc ttcggcgaag
     9241 agcctttgtt ctgcttggct ttccggaaat attggattga caactatgtg accaaacagc
     9301 cggaaaaact gtctgtaatt accgttaaag gcgattcaat ggagggcgtg ctcaatcatg
     9361 gggattccat tctcatcaat cacgccaaaa ccgacccgcg tgacggccta tatgtcttgc
     9421 gtataggcaa tgacctcttt gtcaaacgtg tgcaacgcat accgggcaaa ctgcttgtaa
     9481 cgtctgaaaa tccacgttac gcaccgtttg aaatagattt tagcaatgcc caagacgaca
     9541 tcgccatcat aggccgtgtt gagtggtatg gccggagtat tgattaaatc cattttaaaa
     9601 acctcttaaa acagttttaa aaattcccta atctcgtcag ctttaaacag aaaaccgcgc
     9661 attccggcgc ggttttgtga aaaagctggc gtaacttttc cggatacaaa aaaacgccga
     9721 aatccacgtc tttccgagat ttcagcgttt tttactctat ttgttccttg tgcaaaaact
     9781 aacagtcccc cacaggaaaa cgtcgctctg ttccgccaca acggcgaaat cctgttgagc
     9841 ggcatcaaca tcaaactcaa aagcggcgat tccctgctga tacgcggccc gagcggttgc
     9901 ggcaaaactt cgttgctgcg cgcgctggct gggctttggc cgttcggcag cagcggcaaa
     9961 gtcagccgtc cgccgcatca agacatcctc ttcctgccgc aacgcccgta cacggcacag
    10021 ggcagcctgc gcgacgcggt ttgttatccc gacatcgaca aacagcatcc ggagctaatc
    10081 gaagccatga acacctgccg cttgggttat ctggttgata aattagataa aaccgacgac
    10141 tggcaacaca aactctcccc gggcgaactg caacgcgtcg ccttcgtccg cgccctgctt
    10201 tccaagccca aaatcgtcct gctcgacgaa gccaccgccg ccttggacga accggccgaa
    10261 gccgcgctct accgcgcctt aaagcaaaaa ctgcccgaca gcatcatcat cagcatcgga
    10321 caccgaagca cgctcaacgc gttccacaat atgcgcctcg atgtcggcaa cgttgcctgc
    10381 ggttgaacga tgccgtctga agccgttttc agacggcatc gttcaaattg gaaaggcgcg
    10441 cgaaaacaac gtacaataaa cccgtttacc acccctcgga atgccgaacc gtgaaccagc
    10501 ttattttcga ctttgccgca cacgactatc cgagtttcga caaattcctc ggcacggaaa
    10561 acgcggaact ggtctatgtc ctccgacaca aacacggaca gtttatctat gtctggggtg
    10621 aagaaggcgc gggcaaaagc catcttttgc aggcgtgggt cgcacaggcg ctcgaagccg
    10681 gcaaaaacgc cgcctatatc gatgccgcct ccatgccgct gaccgatgcc gcgttcgagg
    10741 cggaatacct cgccgtcgat caagtcgaaa aactgggcaa cgaagaacaa gccctgcttt
    10801 tttccatctt caaccgcttc cgcaacagcg gcaaaggctt tttgctgctc ggttcggaat
    10861 acacgcccca gcagcttgtc atccgcgaag atttgcggac gcgtatggcg tactgcctcg
    10921 tttacgaagt caaaccctta accgaccaag aaaaaatcga cgcgctcgcc agtatggcgg
    10981 cggcacgcca agtaaccgtc gattccgaaa ttttcgaata cctgctcaaa cactggcggc
    11041 gcgatatgga cagcctgatg atgatgctcg acacgctgga caactacgcc gtcacaatgg
    11101 gcaaacgcat caccctgccg cttttgcgcc agcttttgaa acaacaggaa acccaatgaa
    11161 aaacctcgcc atcttcgacc tcgacaacac cctcatcaac accgattccg accacgcctg
    11221 gccgcaatac ctcatcaaaa aaggcttggt tgacgctgcc gaaaccgaag cgcaaaacga
    11281 aaaattctac cgcgactacc aaaacggctg cctcgacatc gacgcattcc tcaaattcca
    11341 cctcgccccg ctcgcccgtt acagcaaaga agagctggcg gaatttcacc gcgaatttat
    11401 ggcggaatac atcatccccc acatctcgcc tatgcagcgt atgctggtgc agagccacca
    11461 aatggcaggc gacgaaaccc tcgtcatctc atcgaccaac gagttcatca tcacccccgt
    11521 ctgccgcctt ttcggcatta ccaacatcat cggcacacaa ctcgaatccg gttccgacgg
    11581 ccgctacacc ggcaattaca tcggcacgcc cagcctcaaa gaaggtaaaa tcacccgcct
    11641 gaaccaatgg ctcgccgaac gcggcgaaac gctcgaaagc tacggcaaaa cctattttta
    11701 cagcgactcc aaaaacgacc tgccgctgct gcgcctcgtc gacgaacccg tcgccgtcaa
    11761 ccccgatgcc gagctggaaa aagaagccaa agaaaaaggc tggccggttt tgaatttcaa
    11821 atgacgcaat gccgtctgaa gcccgattcg gcgttcagac ggcattgagg ctgaaatcca
    11881 aaaaactcaa agccacagga atttatccgg aaaaacaaaa aacacaggaa ttcatcggga
    11941 aaaacaacaa tctttccacc gtcattccca cgaaagtggg aatcttgatc cgtccgcacg
    12001 gaaacttata tttcgtcatt tgcacccgac ataattcaat actttatttt gcaataaatt
    12061 aacggatgct gacaaattag attggattaa ttaccctatt atccatattg ggttatcggg
    12121 ttgctaattg agcagagcgt agcaaaccat aaagtgagtg tttccaccca ctctgcattt
    12181 atagtggatt aaaatttata gtggattaaa tttaaatcag gacaaggcga caaagccgca
    12241 gacagtacag atagtacggc aaggcgaggc aacgctgtac tggtttaaat ttaatccact
//
LOCUS       DAXHSJ010000047        12195 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000047 DAXHSJ010000000
VERSION     DAXHSJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12195
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.047"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            207..944
                     /locus_tag="ACFM8U_000943"
     CDS             207..944
                     /locus_tag="ACFM8U_000943"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC6334200.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            947..1633
                     /locus_tag="ACFM8U_000944"
     CDS             947..1633
                     /locus_tag="ACFM8U_000944"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC6334201.1"
                     /translation="MAGLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSIG
                     NALARKLDKR"
     gene            1792..2655
                     /locus_tag="ACFM8U_000945"
     CDS             1792..2655
                     /locus_tag="ACFM8U_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6334202.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQVELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2717..3286)
                     /locus_tag="ACFM8U_000946"
     CDS             complement(2717..3286)
                     /locus_tag="ACFM8U_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC6334203.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3444..4304
                     /locus_tag="ACFM8U_000947"
     CDS             3444..4304
                     /locus_tag="ACFM8U_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC6334204.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            4439..4777
                     /locus_tag="ACFM8U_000948"
     CDS             4439..4777
                     /locus_tag="ACFM8U_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334205.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            5145..5498
                     /locus_tag="ACFM8U_000949"
     CDS             5145..5498
                     /locus_tag="ACFM8U_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC6334206.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5488..6354
                     /gene="atpB"
                     /locus_tag="ACFM8U_000950"
     CDS             5488..6354
                     /gene="atpB"
                     /locus_tag="ACFM8U_000950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC6334207.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6411..6647
                     /gene="atpE"
                     /locus_tag="ACFM8U_000951"
     CDS             6411..6647
                     /gene="atpE"
                     /locus_tag="ACFM8U_000951"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC6334208.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6718..7188
                     /locus_tag="ACFM8U_000952"
     CDS             6718..7188
                     /locus_tag="ACFM8U_000952"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC6334209.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7193..7726
                     /locus_tag="ACFM8U_000953"
     CDS             7193..7726
                     /locus_tag="ACFM8U_000953"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC6334210.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAIIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7737..9284
                     /gene="atpA"
                     /locus_tag="ACFM8U_000954"
     CDS             7737..9284
                     /gene="atpA"
                     /locus_tag="ACFM8U_000954"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC6334211.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9309..10184
                     /gene="atpG"
                     /locus_tag="ACFM8U_000955"
     CDS             9309..10184
                     /gene="atpG"
                     /locus_tag="ACFM8U_000955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC6334212.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10222..11619
                     /gene="atpD"
                     /locus_tag="ACFM8U_000956"
     CDS             10222..11619
                     /gene="atpD"
                     /locus_tag="ACFM8U_000956"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC6334213.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVNNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11630..12052
                     /locus_tag="ACFM8U_000957"
     CDS             11630..12052
                     /locus_tag="ACFM8U_000957"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC6334214.1"
                     /translation="MSIMEVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         3003 a         3115 c         3144 g         2933 t
ORIGIN      
        1 ttttgttaat ccactatatt gtccgaaatg gttgcccgct tcctgcctta ttgacaaaaa
       61 aacgctttcc cgataatatc ctacgaaaat taacctgccg atttgacaca gcttgcgggc
      121 ataacagcta aagcgttccg acaatttcag ctttatcttc cgcgcccgtt gtgtccgaca
      181 tcgggctttg ttgtatggga aagacaatga ttattttgga caaggtttcc aagcattacc
      241 aaacgcgcga caagacccgt tttgccgccg tcgagccgac cagcctcgaa atccgcgacg
      301 gcgaaatctt cgggctgatg ggttattcgg gtgcaggcaa atccaccctg ttgcgactga
      361 ttaacctgtt ggaacgcccc gacagcggca aggtcaacgt ctgcggacaa gagctgaccg
      421 cgctcgatgc cgccgcattg cgtcaggctc ggcagaatat cggcatggtg tttcagcagt
      481 tcaacctgtt gaacaaccgc accgtcgccg gcaacgtcgc cttcccattg gaaatcgccg
      541 gatggccgtc tgaaaaaatc aaagcgcgcg ttgccgaatg tctcgaaatc gtcggactga
      601 ccgaacgcgc cggccactat cccgcccagc tttctggcgg gcagaaacaa cgtgtcggca
      661 tcgcccgcgc gctcgcgccc aagccgcaag tcatcctcgc cgatgaaccc acttccgccc
      721 tcgatcccgc caccacgcgc agcgtcttgg aatgtttgga agacatcaac aaacgcttca
      781 acgtaaccat cgtcatcgta acccacgaaa tgagcgtcat cagacgactg tgcgaccgcg
      841 ccgccctgtt ggataaggga aaagtggtgg aaatcgtcga agtacgcggc aaccaaatcc
      901 acgcccgatc cgacatcggg cgcgaactga ttcgggagga ctgaatatgg caggcttaac
      961 attccaacaa gccgtttcca acatcatcgg tatgaaaggc gaaatcgtcc gcgcattggg
     1021 cgaaaccttc gtgatggtcg gcttgtccac cacattcgcc gtcgttttcg gcacgttgct
     1081 gggcgtgttg ctcttcgtaa cttccagccg ccaactgcat tacaacaagc cggtgaactt
     1141 cctgctcgac aacctcgtca acctcatgcg cgccttcccc ttcgtcattt tgatgattgc
     1201 gatgataccc gccacacgcg ccatcgtcgg cagcaccatc ggcccggttg ccgcctcgct
     1261 ggtgttgagc gtgtcgggat tgttttattt tgcccgactg gtggaacaaa acctgcgcga
     1321 agtcccaaaa ggcgtaattg aagccgccgc cgctatgggt gcgcctccga ttgccatcgt
     1381 ctgcaaagtc ctcttgaacg aagcgcgcgc gggcatggtt tccagtatta ccgtgcttgc
     1441 catcgggctt ttgtcataca gcgcggcggc aggcatgata ggcggcggcg gcttgggcga
     1501 cctcgccatc cgctacggct actaccgcta ccaaaccgaa gtcatcgtct tcatcgtcgc
     1561 cctcctcgtg ctgctcgtta tcctgattca aagcatcggc aacgcattgg cgcggaaatt
     1621 ggataaacgt taaacccgaa tgccgtctga acgccaaaac ccccaccgct atccgaaaaa
     1681 tgctataaaa tccccctgtt cgcggcaaat gccgtctgaa cgccgaatcc ggacggcagg
     1741 actccctgcc cgtcattttt gtttgaaact gccacaacat caggagaaaa tatgaaaacc
     1801 ttcttcaaaa ccctttccgc cgccgcactc gcgctcatcc tcgccgcctg cggcggtcaa
     1861 aaagacagcg cgcccgccgc atccgcttct gccgccgccg acaacggcgc ggcgaaaaaa
     1921 gaaatcgtct tcggcacgac cgtcggcgac ttcggcgata tggtcaaaga acaaatccaa
     1981 gtcgagctgg agaaaaaagg ctacaccgtc aaactggtcg agtttaccga ctatgtacgc
     2041 ccgaatctgg cattggctga gggcgagttg gacatcaacg tcttccaaca caaaccctat
     2101 cttgacgact tcaaaaaaga acacaatctg gacatcaccg aagtcttcca agtgccgacc
     2161 gcgcctttgg gactgtaccc aggcaagctg aaatcgctgg aagaagtcaa agacggcagc
     2221 accgtatccg cgcccaacga cccgtccaac ttcgcccgcg tcttggtgat gctcgacgaa
     2281 ctgggttgga tcaaactcaa agacggcatc aatccgctga ccgcatccaa agcggacatc
     2341 gccgagaacc tgaaaaacat caaaatcgtc gagcttgaag ccgcgcaact gccgcgcagc
     2401 cgtgccgacg tggattttgc cgtcgtcaac ggcaactacg ccataagcag cggcatgaag
     2461 ctgaccgaag ccctgttcca agaaccgagc tttgcctatg tcaactggtc tgccgtcaaa
     2521 accgccgaca aagacagcca atggcttaaa gacgtaaccg aggcctataa ctccgacgcg
     2581 ttcaaagcct acgcgcacaa acgcttcgag ggctacaaat cccctgccgc atggaatgaa
     2641 ggcgcagcca aataaggcag tcgtataaaa tgatgccgtc tgaaccgtat ccgtgttcag
     2701 acggcatttt tgtcctttaa tccgccattc cctgccattc cgccgaatcc ggcgtatcga
     2761 ttccgaacag cgacaaagcg tgtgcaacac tgtgcgccac tatgtcgtcc gccgtctgcg
     2821 gtttgcggta catcgcagga acagggggaa acaccacgcc gcccatttcc gttacccgct
     2881 tcatattgtc caaatgggca aggttcagcg gcgtttcgcg caccatcagc accagccgcc
     2941 gcctttcctt caaaaccaca tccgccgcac gcgtcagcag attgtcgccg aagccgtgcg
     3001 cgacagaggc aagcgtccgc atcgaacagg gggcgaccag catcccgtcc gttttaaacg
     3061 taccgctggc aatgcacgcc ccgatattgc cgatcggatg cacgaagtcc gccaaggcat
     3121 atacctcgtc tctcgcataa gccgtttccg aagcgcgcgc catctccgca cctttcgata
     3181 ccacaaggtg cgtttcgata tcttgcgcgc gtaaaagttc caaagccttc acgccgtatt
     3241 ggaaaccgct cgccccactg atgccgatta tcaaacgccg taccatcatc cgcctttccc
     3301 ataaaaccgc ctgcaacggc aaaccggcta ttatagtgaa aaaacagaaa tccgataaat
     3361 acggatacaa attgtcggca acacccaata tccgataaaa tatccgattt aacatcctat
     3421 ctgaataggc acgggagggc ggtatggcaa aagtaaaagg cggattgggg cgcggtttgg
     3481 attcgctgct cgccaacggc gcagacaaca gcagcggcga ccgattgacc acggttgcag
     3541 ttaaagatat ccggcccggc cgctatcagg cgcgtgttca aatcgatgac gaagccttgc
     3601 aggagctggc agattcgatt aaggcgcaag gcgtgataca gcccgtcatc gtgcgcgaac
     3661 acggactgtc ccgatacgaa ctgattgcgg gggagcggcg ttggcgtgcc gcacagattg
     3721 ccggcctgac cgaaatcccc gccgttatca aaaccatcag cgacgaaacc gcgttggcaa
     3781 tgggtttgat cgaaaacctc cagcgcgaaa acctcaaccc catcgaagaa gcacaaggct
     3841 tgaaacgcct tgccgacgag ttcgggctga cccacgaaac catcgcccaa gccgtcggta
     3901 aaagccgaag cgcgatttcc aacagcctgc gccttttaag cctgcccgaa cccgtgcagg
     3961 aaatgcttta ccaacgccgc ctcgaaatgg ggcacgcccg cgcactgctg accctgcccg
     4021 tcgtcgaaca gctcgaattg gcgcaaaagg ctgtcaaaaa cggctggtcg gtgcgcgaag
     4081 tcgaacgccg cagccaagcc gcccttcaaa acaaacgtcc cgagcccaaa aaaactgccg
     4141 ccgccgacat cggccgcctg aatgatttgc tgactgaaaa actgggtgtc aacgcagaaa
     4201 tcaaaaccgc caaccataaa aaaggcagaa tcatcctgca tttcgacacg cccgaaacct
     4261 tcgaccatat cttgaagcag ttgggcatag attaccgacc ttaattttgc gggatatacc
     4321 gtctgaaata tagagaatag ctttccagat tttaagtggg aaatataatt ctattgacat
     4381 ttttttgctt cacgtaaaaa tcgttttcct gttttcattt ttaattttcg aggaaattat
     4441 gaacacacgc atcatcgttt cggctgcgtt cgttgcgttg gcattagcag gttgcggctc
     4501 aatcaataat gtaaccgttt ccgaccagaa acttcaggaa cgtgccgcgt ttgccttggg
     4561 cgtcagccca aatgccgtaa aaatcagcaa ccgcagcaat gaaggcatac gcatcaactt
     4621 taccgcaact gtgggtaagc gcgtgagcca atgctatgtt accagtgtaa tcagcacaat
     4681 cggcgttacc acttccgatg caatttgttt gggaggcgga acgcacaaag gcaaaagtca
     4741 atgcaatgct ttgcttaaag cggcaggccg ttgctaatcc tttattcgga aaaggtcgtc
     4801 tgaaaatatt ttcagacgat cttttattta ttgagcaaat tccccaaact gccgacaatc
     4861 cgaccgataa actgctacaa tttcgcgttg attttttatt atttccacgt aaagcaaaca
     4921 aatcacgtaa gaacattaaa aatgtaaaga aattgcggac aaactcctta atatcctgta
     4981 attatatgta atgtgtgtta gaatgacact tgtcgccgtt attcaggcgg cgagcgcggc
     5041 ggattgggat acgttgtttc acaaagatta actgtcgctt gacgtaataa ggcttctaaa
     5101 ttatattagc cgcgccccaa atccgaggcg gaaacattcc ctttatgaag cagattatca
     5161 tcctacaatc cgccgtattg tccatatgcg ccgcagttgc ctttgctgta tggggttttg
     5221 ccggattcct ttcggcggtc ggtggcggtt tgtcctacct gcttcccacg tttgttgcag
     5281 ttttactttt aaaacttttc aggggaaacc ctttcctgca aagcaggatg ttcgtcttcg
     5341 gagagatttt aaaagtagtg ctgtcgctgt tgtccatgct cgccgtattt gctgtatggc
     5401 atcaatcgtt ggtgttcgcc ccgtttctga tggggctgct cggtgtcagc catttggttt
     5461 ttttagtatt gttgagagtg aaagattatg gcaggtgaaa ccattaccgc tgccgactac
     5521 atcaagcacc acttgcagag cttgaccagt ttgtcggatg ttactcaggg tcagggactg
     5581 aaaaacattg ctgatttttc gtttattaac cttgatgccg tcttttttgc cgtcctgttg
     5641 ggcgtaatcg gcagcttcct gttgtggcgc ggtgcgaaaa aagcaacggc aggcgttccc
     5701 ggacgtttcc aggctgccgt ggaaatcttg tttgagtttg tggacgatat gtgtaagagc
     5761 atcattcaca acgaaaagtc tagaaaagcc gtcgcgccgt tgggtctgac gctgtttgtc
     5821 tggatttttc tgatgaacgc gatggatatg ctgccggtcg atttgctgcc gatggtatgg
     5881 cagggcatta ccggcaacca tcacgccctg ctgcgcgtcg taccgactgc cgatttgaac
     5941 accactttgg cactcgctgt cggcgtgttg ctgatttgta tctattacaa catcaaaatc
     6001 aaaggattgg gcggctggtt tcacgagttg ttcagcgcgc cgttcggtgc aaaactcgca
     6061 cctgcgaact ttctgttgaa tttggtcgag tttctttcta aaaccgtatc ccacggtatg
     6121 cggttgttcg gcaatatgta tgcgggcgaa ttggtattct tgctgatagc cttgcttggc
     6181 ggtgcttggg cggcttccgg cagcgtcgaa gtcatggatc cgattctgtt tgtattccac
     6241 attattgccg gtttggcatg ggcgattttc catattttgg tgattaccct gcaggcgttt
     6301 attttcatgg cgttggcgtt cgtctatatc ggacaggcac atgatgcgca ctgattttcc
     6361 taagtagttt gtttttgtag tttaaccttt gtttctttaa ggagtttaaa atgggtttga
     6421 ttgctatcgc atgtggtttg atcgttgcat tgggtgcatt gggtgcctct atcggtatcg
     6481 caatggtcgg ttctaaatat ttggagtctt ctgcccgtca gcccgaactg atcggtccgc
     6541 tgcaaaccaa actgttcctg attgccggtt tgattgatgc cgcattcttg attggtgtcg
     6601 ccattgcact gctgttcgcc ttcgttaacc cgtttgcagg tgcataatca ggacggattc
     6661 gtccgttccg tacaggcgca gcctgtttga ttaaccccag tacgaaggtt aagtaacgtg
     6721 aatatcaatg caacattatt cgctcaaatc atcgtctttt tcggtttggt atggtttacc
     6781 atgaaattcg tgtggccgcc gattgcaaaa gctttggatg agcgtgccgc aaaagtcgcc
     6841 gaaggcttgg ctgccgccga gcgtggtaaa agcgatttcg agcaggctga aaaaaaggtt
     6901 gcagaactct tggcagaagg gcgtaatcag gtttccgaaa tggttgccaa cgccgaaaaa
     6961 cgtgccgcca aaattgtcga agaagccaaa gaacaggctt cttccgaggc ggcgcgcatt
     7021 gcagctcagg caaaggccga tgtggagcag gaattgttcc gcgcacgcga atccctgcgc
     7081 gagcaggttg ccgtgttggc tgtcaaaggt gccgaatcta ttttgcgcag cgaagtcgat
     7141 gcttccaaac acgcaaaact gctcgatacc ctgaaacagg agctgtaatc ttatggcaga
     7201 gttcgcaacg attgccagac cttatgcaaa ggcattgttc ggtctggctc aggaaaaaaa
     7261 ccaaattgag tcttggttgg gcggactgga aaaacttgcg gcggttgttc aggaagggaa
     7321 ggtggcttca ttgattgacc gtcctgaaac gaatgcttca gaaaaagcag atatcctcat
     7381 cgatttggtc ggtttgaaag acaaggagtt gaaaaacttt gttatcgtct tggccgggca
     7441 gaaacgtttg tcgatattgc cggaagtata tgctcaatat caagacttga ccttatcatt
     7501 caaccatatc aaatctgcca tcatttacag tgcctatccg ttgaccgaca aacaggtcgg
     7561 cgagttggcg caaatgctga ataagcgttt cgacagcgag ctgaaaatct ctgtcgaaat
     7621 cgaaccggag ctgattggcg gcataaaagt tgaagtgggt gatcaggttt tggatttgtc
     7681 tgtacaaggc aaattgagtg ccttgtacac gactatgacg aattaggaga gttttcatgc
     7741 agcttaatcc tgctgaaatt agcgatttga ttaaagccaa gatcgaaaat ctgtctgtaa
     7801 atgccgaagt gcgtacccgt ggtacggtga tttcggtaac tgacggtatc gttcgcatcc
     7861 atggtttgtc agatgcaatg caaggtgaga tgctcgaatt cccgggtaac actttcggct
     7921 tggcgatgaa cctggagcgc gactccgtcg gcgccgtagt gttgggcgag tacgaacata
     7981 ttaaagaagg cgacacggtt acctgtaccg gtcgtatctt ggaagtgccg gtcggacgcg
     8041 aactggtcgg acgcgtcgtt gatgcattgg gtcggcctat cgacggcaaa ggcccgatta
     8101 atacaacttt gaccgcccct atcgaaaaaa tcgcaccggg cgtgattgcg cgcaaatcgg
     8161 ttgaccagcc gatgcaaacc ggtctgaagg cgattgactc tatggttcct gtcggtcgcg
     8221 gtcagcgtga gttgattatt ggcgaccgtc aaaccggtaa aacggccgta gcgctggatg
     8281 ccatcgtcaa ccaaaaaggt acgggtgtta tctgtatcta tgtcgctatc ggtcaaaaag
     8341 catcttctat tgccaacgtg gtccgcaaat tggaagagca tggcgcgatg gaacacacga
     8401 ttgtggttgc tgcaactgca tctgaagcgg cggcattgca atatatcgca ccttactccg
     8461 gttgtacgat gggtgaattc ttccgcgacc gcggcgaaga tgccttgatt gtttatgacg
     8521 atttgtccaa acaggctgtg gcttaccgtc aaatttccct gcttttgcgc cgtccgcccg
     8581 gccgcgaagc ctatcccggc gatgtgttct acctgcactc ccgtctgttg gaacgtgcag
     8641 cacgtgtcaa tgaacacgaa gtggagaaat tgaccaacgg cgaagtaaaa ggcaaaaccg
     8701 gttctctgac tgctttgccg attatcgaaa cccaagcggg cgacgtatct gctttcgttc
     8761 cgactaacgt catttcgatt accgacggtc agattttctt ggaaaccgac ctcttcaacg
     8821 ccggtatccg tcctgcaatc aatgccggta tttccgtatc ccgcgtaggc ggtgctgcac
     8881 aaaccaaagt gattaaaaag ctgggtggcg gtatccgttt ggcgttggcg caatatcgtg
     8941 aattggcggc gttctcgcaa tttgcatccg atttggatga agctacgcgc aaacaattgg
     9001 agcatggcga agtcgtaacc gaactgatga aacagaaaca gttcagtacg ttgaatacgg
     9061 ctgaaatggc tttgaccctt tgggcaatca acaacggttc gtattctgat gttccggttg
     9121 ccaaagctct ggcttttgaa tctgaatttt tgagctttgt ccgtacccaa catccggaag
     9181 ttttggaagc cgtcaatgct tcaggtgcaa tgtccgacga gagcgagaaa acgcttgaag
     9241 cagccatgaa atccttcaaa tcttcttacg cctatcaggc ataaggcttg aagtgaaagg
     9301 agtctgaaat ggcagtagga aaagagattc tcaccaaaat ccgcagtgtt cagaataccc
     9361 aaaagatcac taaagcgatg cagatggtgt caacctctaa aatgcggaag actcaggaac
     9421 ggatgcgttt ggcgcgtccg tatgccgaaa aagtgcgtat ggtgatgagc catcttgcgc
     9481 aaaccaatac cgatcatggt attccgttac tggaatctca tcgggaaatc agacgtgtcg
     9541 gttttatttt gattacgtct gataagggtt tgtgtggcgg tttgaacgcc aacgtgctga
     9601 aaaagttttt ggcacaagtt caagagtatc ggaatcaagg tattgaagag gaaatcgtat
     9661 gccttggcag taaaggtctg atggcgtgtc agagcattgg tctgaatgtg gttgccagtg
     9721 ccgtaaattt gggcgatacc ccaaaaatgg aaatgctgct cggacctttg acagaactct
     9781 tccaacggta tgagaaacat gaaattgaca gaatccatct ggtgtattcg ggttttgtca
     9841 ataccatgcg tcaagaaccg agaatggaag tattgctgcc tatcggtgag aacgtgattg
     9901 gcgattcagc tcccaaatca ccgttcagct gggaataccg ctacgaaccg actgcacttg
     9961 cagtgttgga atatctggtt cgccgctatt tagagtctgt ggtttatcag gcgttgagcg
    10021 acaatatggc atccgaacag gcagcccgca tggttgccat gaaagctgca acagacaatg
    10081 caggcaatgc catcaaagag ttgcgtttgg tatataacaa atcgcgtcaa gctgcgatta
    10141 ccacggaatt gtcagaaatt gtagcaggtg cggcggctgt ctgatgccgt ctgaatcctg
    10201 tacggaaatt aggatacgat aatgagccaa ggcaaaatcg tacaaattat cggtgcggtt
    10261 gttgacgtgg aatttccacg cgacatgatt ccgcgcgttt acgacgcttt gaaattagac
    10321 gaaaacggtc tgactttgga agtccaacag cttttgggcg acggcgtagt ccgtaccatt
    10381 gcgatgggca gctcggacgg tttgaaacgc ggcatgactg tgaacaatac cggtgcgccc
    10441 attactgtgc cggtaggtaa aggtacgttg ggacgcattg tcgatgtatt gggaacgcct
    10501 gttgacgaag caggtccgat cgataccgac aagagccgtg ccatccacca agccgcccct
    10561 aagtttgacg aactgtcttc cacaaccgaa ttgctcgaaa cgggcattaa agtgattgac
    10621 ttgctgtgtc cgtttgccaa aggcggtaaa gtaggtctgt tcggcggtgc cggtgtgggc
    10681 aaaaccgtga acatgatgga attgatcaac aacatcgcca aagcgcacag cggcttgtcc
    10741 gtgttcgcag gcgtgggtga gcgtacccgc gaaggtaacg acttctacca cgagatgaaa
    10801 gattccaacg tattggataa agtggcaatg gtttacggtc agatgaacga acctccgggc
    10861 aaccgtttgc gcgtcgcatt gaccggtttg accatggcgg aatacttccg tgacgaaaaa
    10921 gacgaaaacg gcaaaggccg cgacgtattg ttcttcgtgg acaacatcta ccgttacacc
    10981 ctggctggta ccgaagtatc cgcattgttg ggccgtatgc cttctgcagt gggctaccaa
    11041 ccgacattgg ctgaagaaat gggtcgtttg caggagcgta ttacctctac ccaaaccggt
    11101 tctattactt ccatccaagc cgtatatgta cctgcggacg acttgaccga cccgtctccg
    11161 gcaacaacgt tcgcccactt ggacgcaacc gtcgtattga gccgtgatat tgcctctttg
    11221 ggtatttacc cggcagttga ccctcttgac tctacttcac gccaactgga tccgatggtc
    11281 ttgggtcaag aacactacga cgtggcgcgc ggcgtacagt ccaccctgca gaaatacaaa
    11341 gaattgcgcg acatcatcgc catcttgggt atggacgaat tgtctgacga agacaaactg
    11401 accgtaatgc gtgcgcgtaa aatccaacgc ttcctgtccc aaccgttcca cgttgccgaa
    11461 gtgtttacag gttctccggg caaatatgtc gccctgcgcg ataccattgc cggcttcaaa
    11521 gccatcttga acggcgaata cgaccatctg cccgagcagg cattctatat ggtcggcagc
    11581 atcgaagaag cggttgagaa agcgaaaacc ttaaactgag gaggtcggca tgagcatcat
    11641 ggaagttgag gtggtaagta gcgagcagaa aatctattca ggcgaggcaa catttatcgt
    11701 tgttccgact gtacagggcg aactcggtat ttatccgcga cacgagccga ttatgagttt
    11761 ggtgcggccg ggggctttgc gtttgaccgt tccgggcgag gataaagagg ttttggttgc
    11821 tgtttccggc ggtgttttgg aagtacagcc tgataaagta actgtcttgg cggatgttgc
    11881 cgtccgcagt gcggagatgg atcaggcacg tgcggaagag gcgaaaaaag ccgcagaagc
    11941 gggcatttcc caagctaaag acgataaggc tttggcggaa gcacataaag cattggctgc
    12001 tgcgattgca cagctcaaaa ctttggacta tatccgttcg cacaagaaat aaacttgttt
    12061 ggtttggaaa agcacgaccg tttggtcgtg ctttttattt tccggattta aattaaagac
    12121 tgtgtttgga tatggcgttg gcaggcatag cctgctttaa ggtattcaat agggcagcct
    12181 gccgcctaac cgaaa
//
LOCUS       DAXHSJ010000048        11918 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000048 DAXHSJ010000000
VERSION     DAXHSJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11918
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.048"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(305..1597)
                     /locus_tag="ACFM8U_000958"
     CDS             complement(305..1597)
                     /locus_tag="ACFM8U_000958"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC6334215.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            1675..2643
                     /locus_tag="ACFM8U_000959"
     CDS             1675..2643
                     /locus_tag="ACFM8U_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334216.1"
                     /translation="MKLTLAIPSLNLDKDETRIPLCLHAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(2844..4310)
                     /locus_tag="ACFM8U_000960"
     CDS             complement(2844..4310)
                     /locus_tag="ACFM8U_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6334217.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEAKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4552..6666)
                     /locus_tag="ACFM8U_000961"
     CDS             complement(4552..6666)
                     /locus_tag="ACFM8U_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC6334218.1"
                     /translation="MSHDTESKQASKQASKQASKQASKQASGRVNLLTLSVLSLFCTP
                     YAWGADEYDPEVLPRFILEAAENRDSHVWRGPCSISFDCFGMFRAQLGSNTRSTKIGD
                     DADFSFSDKPKPGTSHYFSSGKTDQNSSEYGYDEINIQGKNYNSGILAVDNMPVVKEY
                     ITKNYGTDLKQAVKSQLQDLYKTRPEAWAENKKRTEEAYIAQFGTKFSTLKQTMPDLI
                     NKLVEDSVLTPHSNTSQTSLNNIFNKTLHVKIENKSHVAGQVLELTKMTLKDSLWEPR
                     RHSDIHTLETSDNARIRLNTKDEKLTVHKAYKGGADFLFGYDVRESDEPALTFEEKVS
                     GKSGVVLERRPENLKTLDGRKLIAAEKADSNSFAFKQNYRQGLYELLLKQCEGGFCLG
                     VQRLAIPEAEAVLYAQQAYAANTLFGLRAADRGGDVYAADPSRQKLWLRFIGGRSHQN
                     IRGGAAADGWRKGVQIGGEVFVRQNEGSRLAIGVMGGRAGQHASVNGKGGAAGSYLHG
                     YGGGVYAAWHQLRDKQTGAYLDGWLQYQRFKHRINDENRAERYKTKGWTASVEGGYNA
                     LVAEGVVGKGNNVRFYLQPQAQFTYLGVNGGFTDSEGTAVGLLGSGQWQSRAGIRAKT
                     RFALRNGVNLQPFAAFNVLHRSKSFGVEMDGEKQTLAGRTALEGRFGIEAGWKGHMSA
                     RIGYGKRTDGDKEAALSLKWLF"
     gene            complement(7591..7836)
                     /locus_tag="ACFM8U_000962"
     CDS             complement(7591..7836)
                     /locus_tag="ACFM8U_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334219.1"
                     /translation="MLKSIEFNSHIRNRLAAYLKGRGLDFQTAMREEKGNKEIASIIH
                     GGLPVLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            7948..8376
                     /locus_tag="ACFM8U_000963"
     CDS             7948..8376
                     /locus_tag="ACFM8U_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC6334220.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSDMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            8388..8864
                     /locus_tag="ACFM8U_000964"
     CDS             8388..8864
                     /locus_tag="ACFM8U_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC6334221.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISELSRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(9214..9702)
                     /locus_tag="ACFM8U_000965"
     CDS             complement(9214..9702)
                     /locus_tag="ACFM8U_000965"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC6334222.1"
                     /translation="MLKITLIAACAENLCIGADNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLHVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYDQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAERTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(9770..10825)
                     /gene="aroG"
                     /locus_tag="ACFM8U_000966"
     CDS             complement(9770..10825)
                     /gene="aroG"
                     /locus_tag="ACFM8U_000966"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC6334223.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWDATEE
                     LLALLAGANKKRMARAG"
     gene            complement(10987..11214)
                     /locus_tag="ACFM8U_000967"
     CDS             complement(10987..11214)
                     /locus_tag="ACFM8U_000967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334224.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            11492..11749
                     /locus_tag="ACFM8U_000968"
     CDS             11492..11749
                     /locus_tag="ACFM8U_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334225.1"
                     /translation="MTTQMCASRDVSKSAKGWSVGIGLNVGKQLTDSVGLEFDPYYRH
                     KTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKSK"
     gene            complement(11731..>11918)
                     /locus_tag="ACFM8U_000969"
     CDS             complement(11731..>11918)
                     /locus_tag="ACFM8U_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6334226.1"
                     /translation="RMGVLREMAEKLGHKVLPLAPYSPELNPIEKVWANIKRYLQTVL
                     SDYARFDDALLSYFDFN"
BASE COUNT         2686 a         3329 c         2933 g         2970 t
ORIGIN      
        1 caaacactgc cggaacggat aaatccggat tcccacccac ttcgtcattc ccgcgaaagc
       61 gggaatccgg tgcgttgggt ttccgtcatt tccgataaac tcctgccgcg ttgtatttct
      121 agattcccgc tttcgcggga atgacggcgg atgggtttcc gtgccgtggt gtccggattc
      181 ccgccggtac aggaatgaca gcagtgcttc cttggcttaa ttttatttta atcaactgca
      241 aatgccgtct gaaaaccggt ttgcagattt tcagacggca ttgttttcgt cgccggttca
      301 aacttcaatc cgattgcgac acggtaaccg gtatgccgcg caagcgcgca agcagcgcgt
      361 ccgccttctg tttctgcgcg gcaaacgccg ccttgtccgc ctgcgtcaat tcgggtgtcg
      421 gcaatgcgac tgaaacagga ttgacgggct gcccgttgat gcgtgcctcg taatgcaggt
      481 gcggcccggt cgaacgcccg gtcgaaccga caaaaccgat gacctcgccg ccgcgcacat
      541 tgccttgtgc ctgcgaaaac gcgctcaagt gcgcgtacag cgtttccacg ccgttggcgt
      601 ggcgtatcat caccgcgttg ccgtatccgc ccttccggcc tttaaaggta atcacgccgt
      661 cggcggaagc cctgaccggc gttccctgcg gtgcggcata atcgatgccc gtgtgcagcc
      721 gccaagtgtg caggatgggg tgcatgcggt agccgaacgg cgaagaaatg cgcgtataga
      781 ccagcggctc gatgttgaag ccgccttttt cctgcaacac cctgccgtct tcatcgtaat
      841 aattgccgcc ccctccgcct tccttgtccg aacggtaata gaacgcctga tgccttgtgc
      901 cgcccttaac gacttcagcc gccaaaatat cgcccgccgc cacctgctgc ccgtggaaat
      961 acaggctgtc gtaaatcagg cgcacggcat cgccttcctt caaaccgtca aggctgaagc
     1021 ggccggcgaa aatcccgctt aaggattcgc ggatttcgac gggcacttcc gcccgcgcca
     1081 gcgaaccgcg cgccgacgtt ttgaccacga ccgaacgcag cgtcggcaaa accttcatat
     1141 ccgcctcaga agccgaccgc cgccatatgc cgcctttctt ttccaaagcg accagattgc
     1201 gctcgccgtc ttcgtcggta aaaaactgca cttcgcgcgc gccgccgtcg ccgccgacca
     1261 aaacatgaac cgactggtca gcacgcaaat gccgcaaatc ggcttcgccg ccatattttt
     1321 ccgtgattcg ggcaatctcg tcccgcgcca tacccgaacg cgccagcacg tccgccagcg
     1381 agtcgcccgg ctgcaccgcc tcctgcaccc aatatgccgt ctgaacaccg ctgccgcccc
     1441 aagacagcgg cggcagattt tgttccacgc gctgcggtct gacgcgctcc gtcctctctg
     1501 tcgaagcaat gtatgccgct gacaccaaaa taatcgaaac ggcaagcgcg cgcagcgcgt
     1561 atttccgatg ttttgccgaa agtgggaaga cagccatatt gttccttttt gacggcgttt
     1621 gcgccgaatc ctctataatg ttgcgcctga cctgcatttc tgaaacgtca aacgatgaaa
     1681 ctcaccctcg ccattccctc gctcaatttg gataaagacg aaacccgaat acctctctgc
     1741 ctgcacgcct tcaacaaaat cctgcaatac ggctcgctgc accgccaaag ctgcacggct
     1801 tcggcatttt acgcgcgtta tttgtggtgc ggcagactgg cggaacgcgc ggcgcaaagc
     1861 ctgaatatgc cgtctgaaac cgtcgcgctc gccacgcccg tctggcaaaa gatggggatg
     1921 catcaggcaa atgtgctgac ggcggaatat ttggatgtcg gtacggatga agcggaacgg
     1981 ctctgccgcg atctgtcggc gttttacggc gatattccgt ggcgtttcgt tcccgtcctg
     2041 cccgaattat ggctggtttc ccttccccgc gcgtaccgct ggggcgcgaa gcccgttttg
     2101 gacttgggcg gactgttggg tgcggacgat caaccggacg gggaagacgc gttggaatgg
     2161 ctgcgcgtcc aaaccgaaat ccaaatgtgg ctggcggcgc accccgtcaa ccataaccgc
     2221 aagaaacgcg ggctgcccga gttgaacggc ttatggcttt gggacagtct gcacggaagc
     2281 agtcaaggcg gcacgctttt cgccgatacg gtttggagcc gtttccaccc cgaccgccgc
     2341 gcattgcccg acaacttccg cgcctatgaa gaaacggcgg cacacctgcc cgacacgcac
     2401 catatcctgt ttatggacga tttgcgcctg accgccctga caggggacag ggagcggtat
     2461 gcggcaatat tgcagcagtg ggaagaacgc tggttcgcac cgctttacga agccgtccgc
     2521 atcggcaaaa tcaaacggct ggacatcgcc acggacggac aacacggcgg tacgctgacc
     2581 ttcaaacccg ccgacaggtg gaaattttgg cgcggcgcga aaacctttga cggaatctgg
     2641 tgaacccgcc cgccgcaacc gcgacaatgc cgtctgaagc ccgaatcggg tttcagacgg
     2701 cattttacct cgcatcgccg ttatgccgga aaaaccggaa tgcgtttccg cacacgcggc
     2761 ggcacgcaaa gcaccgcccg aaagccgccg atgcccgccg tgataccgcc gccggcaggg
     2821 tttcctccga aaaggaacag caatcagaac gttttattaa actcgacaaa cgcccgattt
     2881 ttctcgtatt cgttgaacac atcgttactc cgcgtttcgc ggtgcgacaa cgtcaggcgc
     2941 ggcgtgatgc ctttgaaatg caatgcccgg tgccaaaggc tcaaggatgt gttcaattct
     3001 ttatccctgc gcctttcccc tttaaaaccg ctgaaaaagc cgggtttttc ataatgccgt
     3061 ttcgccgcgc cgaggcgcaa cagcgaagac aggccgctgc cgccccattc ctgcccccag
     3121 gcaaagcgca ggccgtaacg gttgaaattg tcgccccggt cggcggggtt gcgctcgcgg
     3181 taaaaatcca aaccgcccat ccaatattgg cgcgcattcc ggtaaaacac cagcgaattg
     3241 gaaatttgca aatgggtatt gtcggaacgc gcccggcgcg tattcttcaa acgcccccac
     3301 tccgccgaag acaacgtttg ccatttcggg gtttgccaac ggttgaaata aaggcgtgcg
     3361 ccgttggtgt aagaataagc gtcgttgccg taggtgcggc gttcgtggaa cactgccagc
     3421 ccggcatctt tgcgccggtc ggcaaaaccg atgccgccgg aaacgcctgc cgtcatatcg
     3481 ttgaatttct tattccccgg ataaaccctg ccggacacgt cgccgcccgc cgtcgtgtac
     3541 cagccgtttt tcagcgacca ttttttctcc gcgccgagcc ggtaattgac cgccgtgccg
     3601 tccacctgtt tcgggaaagt ccatttgccg tactgctgcc gtttcggggc ttggttgata
     3661 ttgtgttcgc gggtgacgct gaagccgcca tttaccttcc acgcatcgcg ttcgcgcaat
     3721 gccttgcggt acagctcgac ctgctccatc agctgcggcg gcaggttttc cgccttcagg
     3781 cggtcgaact ggtctgccgc cgcctcgttc tgcctgtttt caaacaatgc cgccgccaaa
     3841 cgcatacgga cggcgggcgc gtcgggttgg gcggcaatca attcccggta atgggaaatc
     3901 gcctccttca ccctaccgtc tgcctgcgcc aaaatccctt gtgcataaag tgccaacatc
     3961 ttatcctgct gcgcctgttg taggtaaatt ggcaaaataa cgcggatacc ggcaatattg
     4021 tttgaaacca ctgcggaata catcgcgcgg gacagtaatt cgggattttt cagcagggtt
     4081 tcgccgtcaa tctgcaaaac ttttcctttt tcccgcacct gccccggtac cttctccctg
     4141 tcgatcggtt tggcctccgc ttcatgaagc ctgaactcgg gacggcttct caaatccggt
     4201 tcgcgcggtg tttcttcggc atatgccgcg cttgccaata aaggcagcag cagcatccat
     4261 ctgtttcgtg caggcataaa tgtcttccca caaaaataaa aaataaccat tatcaaccgg
     4321 cggattgtat cagtttttta tgccgctgtt ccaaaccctg ccgcccgatg ccgcgacaat
     4381 gccgtctgaa gccagaatcg ggtttcaaac ggcggcaaaa aaccggcggt tttccgccgg
     4441 tttcatcatt atgccccgcc cttcggggcg gcagggttcg gattggtttg cggggcggcg
     4501 cgcggtgccg tgccggtgtg ccgcctgtac gtcaaaacat ttcccggcgc atcaaaacag
     4561 ccatttgagc gacaatgcgg cttctttgtc gccgtccgtc cttttgccgt atccgatgcg
     4621 tgcggacata tggcctttcc aaccggcttc aatgccgaac cgcccttcga gtgccgtcct
     4681 gcctgccagc gtctgttttt cgccgtccat ttccacgccg aaagattttg acctgtgcaa
     4741 aacattaaaa gcggcaaaag gctgaagatt gacaccgtta cgcaaagcaa aacgggtttt
     4801 tgcccgaatg ccggcgcggc tttgccactg accgctgccg agcagtccga ccgccgtccc
     4861 ctcgctgtcg gtaaagccgc cgtttacgcc caagtaggta aactgcgcct gcggttgcag
     4921 gtaaaaccgc acattattgc cttttccgac aacgccttcc gccacaagcg cgttgtagcc
     4981 gccttcgaca gaagccgtcc aacctttggt tttgtagcgt tccgcacggt tttcatcatt
     5041 gatgcggtgt ttgaaacgtt ggtattgcaa ccagccgtcc aaatacgcac ccgtttgttt
     5101 atcgcgcaac tgatgccacg cagcataaac acccccgcca taaccatgca aataactgcc
     5161 tgccgcaccg cctttgccgt tgactgatgc gtgctggcca gccctgccgc ccatcacgcc
     5221 gattgccagc cggctgcctt cattttgccg tacaaacacc tcgccgccga tttgcacgcc
     5281 tttgcgccac ccgtccgcag ccgcgccgcc ccgtatattt tgatgcgacc gaccgccgat
     5341 gaagcgcagc cacaattttt gacgggacgg atcggcggca tacacgtcgc cgcccctgtc
     5401 ggcggcacgc agcccaaaca aagtatttgc cgcataagcc tgttgggcat ataaaaccgc
     5461 ttccgcctca gggatagcca aacgctgcac gcccaagcaa aatccgcctt cgcattgctt
     5521 gagcaataat tcgtacagtc cctgccggta attttgttta aacgcaaacg aattagagtc
     5581 tgccttttcc gccgcaatca gtttgcgccc gtcgagcgtt ttcagatttt ccggccggcg
     5641 ttccaaaacc acgccggatt ttccgctgac tttttcttca aaggtcaggg cgggttcgtc
     5701 cgactcccgc acgtcgtagc cgaacaggaa atccgcaccg cccttatacg ctttatggac
     5761 ggtcagtttt tcatctttcg tgttcaggcg gatgcgggca ttatcggaag tttccagcgt
     5821 atggatgtcg gaatggcggc gcggttccca aagggaatct ttcagcgtca tcttggtcag
     5881 ttccaacacc tgtccggcga cgtgggattt gttttcgatt ttgacgtgca gtgttttatt
     5941 gaagatgttg ttgagactag tctgtgatgt attactatga ggagtgagta cggaatcttc
     6001 taccaattta ttaattaaat cgggcatcgt ctgtttgagc gtactaaatt ttgttccaaa
     6061 ctgtgctata tacgcctcct cagtccgttt tttattttct gcccaagctt ccggtcttgt
     6121 tttgtataaa tcctgtaatt gacttttaac cgcctgcttt aaatcagttc cgtaattctt
     6181 tgtaatatat tccttaacaa caggcatatt atcgacggcg aggatgccgc tattgtaatt
     6241 tttaccttgg atattgattt cgtcataccc atattcggat gaattttgat cggttttacc
     6301 gctggaaaaa taatgggaag tgccgggttt cggcttgtct gaaaatgaaa aatcggcatc
     6361 gtcgccgatt ttggtagaac gagtatttga accaagctga gctctgaaca taccgaagca
     6421 atcaaaagat atgctgcacg gaccgcgcca aacgtgggaa tctctgtttt ccgccgcttc
     6481 taaaatgaat cgcggaagaa cttcaggatc atattcatcc gcaccccaag catatggcgt
     6541 gcaaaacagc gataaaacag ataatgttaa tagattaacc cgaccgcttg cttgcttgct
     6601 tgcttgcttg cttgcttgct tgcttgcttg cttgcttgct tgcttgcttt cagtgtcatg
     6661 agaaattttc ctttgtcaag tgttaaaatt ataatgatta tatactacat cgcactacat
     6721 cgcaatgaaa aatcaggaaa aactaaaaac ccctccgccg tcattcccgc gaaagcggga
     6781 atctagaatt ttaatgccgc aagaatttat caaggatggt cggggtttaa aggtctggat
     6841 tcccgcctgc gcgggaatga cggtttagaa atcacccaaa acaagaaaaa gaaccgaaac
     6901 cgaacaagcc ggattcccgc ctgcgcggga atgacgggat ttcaggttac tgtttttgat
     6961 tttctgtttt gtggaaataa cgggattttg gatgatgtgc atttatcgag aaaactaaaa
     7021 accctccgcc gtcattcccg cgaaagcggg aatctagacc tttcggtgcg gaaacttatc
     7081 ggataaaacg gttttccctg catcctggat tcccactttc gtgcgaatga cgggatatag
     7141 gtttcactac ggacgtgttc agattaccgc ctgcgcggga aggacgggat ttcaggttgc
     7201 tgtttttgat tttctgtttt tgtgggaata acgggacttt ggatgatgtg catttatcgg
     7261 gaaaacaaaa aaccctccgc cgtcattccc gcgaaagcgg gaatctagaa ttttaatgct
     7321 gcaagaattt atcggggatg accgaggttt gaaggtctgg attcctgcct gcgcgggaat
     7381 gacggtttag aaatcacccg aaacgagaaa aagaaccgaa accgaacaag ccggattccc
     7441 acctgcgcgg gaatgacggc tcaaaagtta cgaaacgaaa aataaccgaa accagacaag
     7501 tcggattccc gcgcgggcgg gaatgacgat aagcgtaatt tcaatctgcc atattgtctt
     7561 ttcacttgcc tgaaaacaga tttcagccgc ctatccctgc atccggcggc tgatgtagtc
     7621 ggcaatcaaa tcccgctttt cccaaaaaaa tttctgcatt ttttgttcgg aatacagttt
     7681 gcgaaccagt acgggcaggc cgccatgaat aattgaggca atttctttgt tgcctttttc
     7741 ctcccgcatc gccgtctgaa aatccaaacc cctgcctttc agatatgctg caaggcggtt
     7801 gcggatgtgg gaattgaatt cgatggattt gagcattttg cctccgtttt gcggtaagga
     7861 tttattcgat ttgtttatcc gcctgatttt atcacgtttc ccaagcggtt tgcctgtaca
     7921 ataccgcttt tcggcagggt gtgggcgatg atgttttctt ggttcaagct gtttcacttg
     7981 ttttttgtca tttcgtggtt tgcagggctg ttttacctgc cgaggatttt cgtcaatatg
     8041 gcgatgattg atgcgccgcg cggcaatccc gagtatgtgc gcctgtcgga tatggcggtg
     8101 cggctgtacc gttttatgtc ccctttaggt ttcggtgcgg tcgtgttcgg cgcggcgata
     8161 ccgtttgccg ccggctggtg gggcagcggc tgggtacacg tcaaactgtg tttgggcttg
     8221 atgcttttgg cttaccagtt gtattgcggc gtgctgctgc gccgttttca ggattacagc
     8281 aatgcttttt cacaccgctg gtaccgcgtg ttcaacgaaa tccccgtgct gctgatggtt
     8341 gccgcgctgt atctggtcgt gttcaaaccg ttttgacgga gcatcttatg ccgatagaaa
     8401 tcgaacgccg ttttttgatt ggaaacgaca actggcggca atacgccgac gaaccgctgc
     8461 tgttgaggca gggttatctg tctgttgaaa aagaacgcac cgtccgtgtc cgtattgccg
     8521 gacaacgggc gtggctgacg ctgaaaggct atatttcgga actcagccgc agtgagtttg
     8581 aatacgaaat cccgcttgcc gatgcggaaa agatgatgga aacgatgtgt ccgtttaaga
     8641 tggaaaaatg gcgttatccg gtcagatggg gcggcagcct gtttgaaatc gatgtttttc
     8701 ttggcgataa tgcgcctttg gtcgtcgcgg aaatcgagtt gcccgacgag aatgccgatt
     8761 ttgaccgtcc ggactggctg ggacgggaaa tcactgccga cggtatgttt accaatgcct
     8821 atttgagcag gcatccgttc tcaagctgga aaaatgccgt ctgaagcggc ttcagacggc
     8881 attctccgac aaccatcgca aatccaaaga cagcctctat atccttattt ctgctttctt
     8941 tgttccatta ttcccaataa atccccataa cctaaaatct cgtcattccc gcgcaggcgg
     9001 gaatccggtt ctttaaactt cggttttttc cgataaattg ctttagcttt tcgtttccgg
     9061 attcccgctt ttgcgggaat gacgatggaa gattgttgtt atttcggata aattacccta
     9121 actttaaaat cccggattcc catcttcggg ggaataacaa tattttctat ttcagcccat
     9181 catttgcggg ggattttata gagagtttat atttcaatat ctcaaataat gcacaaaagc
     9241 atagctcgtg cctttgctgc tgacacggcg ttccgtccgc tctgcttctt tccaatgcgt
     9301 ccggtctatt tcggggaaaa acgcatctcc ttccaccgac aaatccactt cggttatccg
     9361 caaatcggtc gccaatggca ttgcttggtc gtatatctgc gcgccgccca taatgacggc
     9421 ttcttccgcg cctgcgcaca atgccaatgc cgcctccaaa cttgccgccg tttccgcacc
     9481 tgccgcgcaa taatccgcct gcctgctgat gacgatgttc ctccgtccgg gcaggggttt
     9541 gacgtgcagg gattcccacg ttttccgccc cataatgacg ggtttgccca aggtataggc
     9601 tttgaaaaat gcaaaatctt cggggatgtg ccaaggcata gcattgtccg ccccgatgca
     9661 caggttttcc gcacacgccg caattagggt tattttgagc atatttcctc cacaaaaatg
     9721 ccgtctgaac gcttcagacg gcattttatg acattctgcg tcaaaaatct caaccggcgc
     9781 gcgccatacg ttttttgttt gcacctgcca acaatgccaa cagttcttca gtcgcgtccc
     9841 aaccaataca cgcatcggta atgcttttgc cgtacacttc cggcttatcc tgtctgcctt
     9901 cgaccaaatg gctttccacc atcacgccca taatattgct cccgtcctgt tccaattggg
     9961 cagcaatatc ttgtgccact tccatctgcc gggtgtaatc cttgcggctg ttggcgtggc
    10021 tgcaatctat catcagctta tcggttaccc ctgccgcacg cagttgttcc gccgcctcgc
    10081 tgacgtgtcc cgcatcataa ttcggctctt ttccgccgcg caaaatgaca tgacagtcgg
    10141 gattgccgcc ggtatggaca atggcggaat gtccggcctt ggttacagac aagaaatgat
    10201 gcgaatggct cgccgcaccg attgcgtcga tggcaatctt caaattgccg tccgtaccgt
    10261 ttttaaagcc gacggggcag gacagcccgc ttgccaattc gcggtgaact tggctttcgg
    10321 tcgtccgcgc accgattgcc ccccaagaaa tcaagtccgc ataatattgc ggcgtaatca
    10381 tatccaaaaa ctcggtagag gcaggcatac ccatattgtt cagcgacaac aacaggctgc
    10441 gcgcctgacg caaaccgaaa ttgatatcga acgtaccgtc caaatgcggg tcgttaatca
    10501 aacctttcca acccaccgtc gtcctcggct tctcgaaata aacgcgcatc acaatcaaaa
    10561 gctcgttttc atactgcttg cggagtttca acaaacgctc cgcatattcc aacgccgctt
    10621 tcggatcgtg aatcgaacac ggcccgataa taaccaacag ccgcttgtcc ctgccgtgaa
    10681 ccaaatcgga aatttcctga cgggtgcggt gaaccaagcc cgaagcctct ttggaaatcg
    10741 gcagctcgta aagatgggca atcggcggca acaactcttt aacttcttta atcttaatat
    10801 cgtctgtggg gtaatggtgt gtcatcttgc atctttcctg atggtttttt atacgcggct
    10861 taggttacat cgtttacctt gcacaaacaa gtgcaacata ttactacaaa gctattttat
    10921 ttcaaaataa ctgatatatc ttttttcata gattttaaat ttcatatttt taatatttta
    10981 ggaattttat tccaaagaaa aagaaacaga ttgataaaaa caatacccgc cattcattcg
    11041 gacaaaaccg cacggaacat ccgttttccc atttccacga caatccggta cagaacaccc
    11101 cgctgccgat tccgcctgca catccggcaa aatttcatac gcaaggaaaa tgttgaaaac
    11161 aaaaagaact aattgcttga aaaaagattt aattggaatg cgggtatttc ccacactttc
    11221 tccatttatt catcctcatt ataggaagaa aaacatgaaa aaaccctgct tgcactctca
    11281 cttgcagccg tgtccgccgt tacggtagcc gatattcctg agtctaacgg caagggcaaa
    11341 gccatcatcg gcgataagga aaaacaccgt taccaagcat gaaaaccttg ttttgaaagt
    11401 ctatggcgaa atccgccaag atttggggga cggatgatcc ctgacccctt gggcggatgc
    11461 atatgcagat ttgcgcggca aaaccaaagt gatgacgacc cagatgtgtg cttcccgcga
    11521 tgtcagcaaa agcgccaaag gttggagtgt cggtatcggt ctgaatgtag gcaaacagtt
    11581 gaccgacagc gtcggtctcg agtttgatcc atactaccgt cacaaaacaa tctgcaaacc
    11641 ccgtgagatt gttttggacg gcgacaaaac caaaatgggc cgctccaaat ccaacgagta
    11701 cggcttccgc gtaaccgcaa cgttctatag tcaattaaaa tcaaagtagg acagtagtgc
    11761 atcgtcaaat cgggcgtaat cagacaaaac ggtttgcaga taccgcttaa tattcgccca
    11821 cactttctca atcgggttga gttcaggtga ataaggtgca agaggcaata ccttatgtcc
    11881 caatttttcc gccatttccc gtaagacacc catacggt
//
LOCUS       DAXHSJ010000049        11699 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000049 DAXHSJ010000000
VERSION     DAXHSJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.049"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(308..730)
                     /locus_tag="ACFM8U_000970"
     CDS             complement(308..730)
                     /locus_tag="ACFM8U_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC6334227.1"
                     /translation="MQVTSKWIDGMCFVGTTESGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(848..1519)
                     /locus_tag="ACFM8U_000971"
     CDS             complement(848..1519)
                     /locus_tag="ACFM8U_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC6334228.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1535..2161)
                     /gene="purN"
                     /locus_tag="ACFM8U_000972"
     CDS             complement(1535..2161)
                     /gene="purN"
                     /locus_tag="ACFM8U_000972"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC6334229.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDRLNHKNFTSRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2268..3086)
                     /locus_tag="ACFM8U_000973"
     CDS             complement(2268..3086)
                     /locus_tag="ACFM8U_000973"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6334230.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGTVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3345..3785)
                     /locus_tag="ACFM8U_000974"
     CDS             complement(3345..3785)
                     /locus_tag="ACFM8U_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC6334231.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSETSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3848..5254)
                     /locus_tag="ACFM8U_000975"
     CDS             complement(3848..5254)
                     /locus_tag="ACFM8U_000975"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC6334232.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTDNGLQAIAVCRLKSTDRAALNKVAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASRNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5425..6540
                     /gene="lptF"
                     /locus_tag="ACFM8U_000976"
     CDS             5425..6540
                     /gene="lptF"
                     /locus_tag="ACFM8U_000976"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC6334233.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFAVALILLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6537..7607
                     /gene="lptG"
                     /locus_tag="ACFM8U_000977"
     CDS             6537..7607
                     /gene="lptG"
                     /locus_tag="ACFM8U_000977"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC6334234.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7907..10492
                     /gene="acnB"
                     /locus_tag="ACFM8U_000978"
     CDS             7907..10492
                     /gene="acnB"
                     /locus_tag="ACFM8U_000978"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC6334235.1"
                     /translation="MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10634..11629
                     /locus_tag="ACFM8U_000979"
     CDS             10634..11629
                     /locus_tag="ACFM8U_000979"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC6334236.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         2676 a         3236 c         3051 g         2736 t
ORIGIN      
        1 aataaattaa tatatggctt aaaataacgg gatgcccgcc ttccgcccgc ccgcagaagc
       61 aggcgggtat cattttaaaa cgcggcattt aaaatttgac cgaaaattgt tgacaatccg
      121 gaatcaagtc tgcacaatac cccgacaagt ccaagtatta taaaggctga ataaagagga
      181 aacagcaggc agatatattc gggaggtgca gtccgaatat atctgctttt ttatgcgcct
      241 ccggattgcc tgccgcacct ttcccttcag acggcatcag ccgtttcccc ataatgccgc
      301 ccgatgccta tttatctgcc ccggcaattt caaaactgtg ggtaatcttt gccgctttgc
      361 ccaacataat cgaagccgaa cagtattttt cggcagacat ctgaacggcg cgctcaatgg
      421 ccgattcttt caaatcatgc ccgaatactt tgaaatggat gtggatttcg gtaaacacgc
      481 gcggcgcatc gtccgcccgt ttcgccgtaa ccgtcgcacg gcagtcagtc actttctgac
      541 gctgtttttc ggcaatcatc accacatcga tgctcgaaca gcccgccacg cccaacagca
      601 gcatttccaa agggctgggc ccgcgcttgg ccgcaccttc tgccgccgaa ccctccataa
      661 cgacgctgtg cccgctttcc gtcgtgccga caaagcacat tccgtctatc cattttgatg
      721 taacctgcat ggtgtcattc ctgaaaatag cgttaaaacc gctttgcata tggcgttatt
      781 gtaaacaatt tcaagcggct tatgcagaaa tatggacaaa acggcaaaaa aacgcctgaa
      841 aaccgattta cggtttggcg gactggccat tgatctgcac cgatttgagt ttcagcgtat
      901 aggttttgcc gtcgtcggta tagccgattt gtgccggaat attgttcagg gacggtgcga
      961 agaaatacat taccgcatcg tcgccgcgcc gcacccgata tttgacgact tcggtttcca
     1021 cgccgcctat gctgtatttt cctgtacccg ccttattcaa accgccgacg gaataaagtt
     1081 ttttgccgtt ggtgattttc agccccggtg ggagtttcgc gtcatttgcc gccaactgcc
     1141 aggcaagcgt gaacaaatcc atagccttgg ggctttgctc ggttttgctc tcgcccgctt
     1201 tgccgtaagt tacgctgccg tcggcgaatt tggcttccgc atacagtttg cccctgcgta
     1261 tgtctctata gtaggtaggg tgcagggtat tgccgacaac cgtaccgccg gactcgaaac
     1321 ggatattgta tagcggcact ttaatcgtcg aaacgatttt gtaagcattg ccgctgcgtt
     1381 caaatgtcat cgtggcggga atgccgtagc tgccggaata gtgcagcacg gcggattggg
     1441 gcagccctgc cgcatacgcg cacggcaggg cggcggacaa aatggcggcg gaaaatatat
     1501 ttttaaaagt cttcatcatt tgctcccgcc cggtttacgc cgtcaggaaa cgggcggcat
     1561 cggcgttttc cgaatttctg acgcggtttc cctcgataat caggcggccg gcggcaaaat
     1621 cggcaacggc tttcggatag agtttgtgtt cgacagccaa aacccgggcg gcaatatcgt
     1681 ctgccgtatc accatcaagt atcggcacaa tcccttgcga tacaatcggg ccgcaatcca
     1741 gctcggccgt aacaaagtga atcgtacagc ctgcaacgcg gcaacccgct tccaaagcgc
     1801 gttcgtgcgt atgaagtccg gtaaacgagg gaaggatgga cgggtggatg ttcatcagcc
     1861 tgccttcgta atgggcgcaa aactcggggg tcagaatccg cataaaacct gccaaaacca
     1921 ccaagtcggg ttgatatgcg tcgatttttt ccatcatggc ggtatcgaag gcaagccggg
     1981 atgtaaagtt tttatgattc agtctatcgg tcgggatgcc gcgttcggcc gcccattgca
     2041 aaccggcagc cgtttcgctg ttgctcaaca cggcggcaat gcggacgttg ggaatagcgg
     2101 cattgacgat tgcctgcatg ttgctgccgc gcccggaaat caggatgacg atgtttttca
     2161 taatggtgcg cttttgaaag ggatgccgtc tgaaccgctg tttggtggtt tcagacggca
     2221 tttgccgtaa aaatgcccga aaacctgttt cgggcatgga ttcggactta atttactttt
     2281 ttgatgtcga cttgagccgg ctgcttggcg ggcgcgtttt cgggtgcgcc gattttgacc
     2341 agtttcacat caaataccaa agtggcgttc gggccgattt tgtcgcccgc accctgttcg
     2401 cggtaggcaa ggttggacgg gatgtagaac gtggcttcgc cgccttcttt cagaagttgt
     2461 acgccttcgg tccaacccgg aatcacttgg ctcaaaggga aggtgaccgt gccgccgttg
     2521 gctttgctgc tgtcgaatac cgtaccgtca atcaggcggc cttcgtattc cacggtaacg
     2581 atgtcgtctt tggtcggctg tttgccttca ccctgtttgg tgattttgta ttgcaggccg
     2641 gaagcagtgg tcttcacgcc gtctttggcg gcattttctt tcagaaaggc ttcgcctttt
     2701 tctttattgg ccttcgcgtc cgccttgtgt ttttctacgg ctttagcctg ttgttcctga
     2761 aggaatttca tcatgacttc ctgagcctgc tcttcggtca ttttgatttc tttgccgtca
     2821 tacactgcct gcatggcttc ggtaaagact ttcaaatcga tttccgcgcc ctgttccttc
     2881 atttgcttca gggagcgtcc gatgtccacg cccatcgcat agcttgcctg ctgcatcgtg
     2941 ctgccgatcg aagaggtgtc gccctgcgcg gaagaagcgg cggcaggttc ggatgcagat
     3001 gcgggggcgg cttctttttt gccgcaggcg gaaagtgcca aagcggcgga aagggtcagt
     3061 gcgctgattt tgaaaatggt gttcatgatg gatcttcgct gtcgataagg tcggaaaaac
     3121 gggattatag ccgagtttga atgtttcaac acacaggatg acacataaag cgtcaatcgt
     3181 gtgttgccct gttttggaag ggattgaacc ttccaaaata agttttgatt ctaccgcccc
     3241 gagggacaga tgtccaagtg gcggggttca accgataagg aaattttaat caaatagaat
     3301 caagcctgtt taaattttgt aaatgcggca tttcagacgg cattttatgc cttgccctcc
     3361 atgccgtgat gttcgatggc aaaaccgctt cggcggtagg cggtaaagcg ttcgcgcgcg
     3421 tcggcgagat cttccaagct gttgccgacg atttccaaaa cgcgcgcggg caagaccgga
     3481 gcggtgttcc aaaaaccgtc ggacaggttc aaaacggtca tgccttcggg aattcggggc
     3541 agattgccgc cgcaggcaag caggacggat gtttcagacg gcatggcttc ttccgtttcc
     3601 caaatttcgt gcggaatgaa actctcggcc tcgtattgcc aaagcatttt gtccaattcc
     3661 tgaagctgcc cgaacgaatc ggaccacacc agtatcctgc cgccgtcccg aatcgcacgg
     3721 gcaatcaggc ggcaggtgaa aatcggaact tgggcaacgt gagtgtaaaa ggtggctttc
     3781 ggcatattgt ttgaacattt ggcaggataa tgccgtctga aaggcttcag acggcattgt
     3841 ggcaaaatta aagattccgc agatagttca gcagcaaggg aacgggacgg ccggtcgcac
     3901 ctttttccgc accagatttc cacgccgtac ccgcgatgtc aaggtgtgcc catggatagt
     3961 cttcggtaaa gtaggatagg aatgttgcgg cggtaatcgt gcccgcgccg ggcgtgccga
     4021 tgtttggaat gtcggcaaaa ttggacttga gctggtcttt gtaggtttca aagagcggca
     4081 gttgccacgc tttgtcgtcc acgttgcggg aggcggcgag caggctgtcg atcaaatcct
     4141 gattgttgcc catcacgccg ctgacatcgt gccccaaggc gacgatgcat gcaccggtca
     4201 gggtggcgac gtcgatgacg gctttgggtt tgaactgttc ggcgtaagtg agtgcgtcgc
     4261 acaaaatcag acggccttcg gcatcggtgt tcaacacttc gatggtcagc cctttcatgc
     4321 tttttacgac gtcgcccggt ttgttggccg cgccggaagg catattttca caagtggcga
     4381 cgacggcaat caggttgatc ggcagttgca gtttgacggc ggcgcagaag gtgctgatga
     4441 cggttgccgc gccgcacata tcaaacttca tttcgtccat gttcaggccg ggtttgaggg
     4501 agatgccgcc ggtgtcgaag gtaatgccct tgccgaccaa taccacaggc gcggcttctt
     4561 tgtcggctgc accgaagtag ctcagttcga ccaaataggg atcttcgaca ctgcctttgg
     4621 cgaccgacca aaacgaaccc atgttttctt tgatgtagtc tttttcgatg attttggcgt
     4681 gcgcgcccag tttttcggct tcggctttgg cggtgcgcgc taaaaattcg ggcgtgcatt
     4741 cgttcggcgc agcattgccc aagtcgcggc agaggctttg tccgtaaacc tgtgcttcgg
     4801 cgacgcgcaa ggcttcttta acggcggctt cgtgtgcgct gtggaacacg gcggtttcaa
     4861 atttggcggg cttggcttct tttttgtagc ggtcgaaacg gtaggcggca ttgccgaacg
     4921 caatcgcaaa cgcttcggct acggcggcgg cttgcgcttc atcgaaggca tggatgtcca
     4981 cattgaccgt ttcctgattt tgcgcccatt tggcggcttc ggctgcgact ttgttcagcg
     5041 cggcgcggtc ggtgcttttc agacggcata cggcaatggc ttgcaaaccg ttgtcagtcg
     5101 ggatttttgt gtcggcaaaa ttttgacctt cttcaagcga agacaaaagg gcaagggcgg
     5161 tcggatggtt taattgcgcc tcttcggtgc agacaaataa ctgtgcgcct gcctgctgtt
     5221 cctgcaagat ttcggttttt gtgctaaatt ccacgtttat tctcctgatt gagacggttg
     5281 tcggtagttt tcggacggcc tttcgctcaa aagaccgtct gaagacggct ggcacgattg
     5341 taccccattt gaagcaccgt ctgaaacctt gcgcggacaa tccgcctgcg ccgaaccgct
     5401 taccgccccc ctgaccgcga ttctatgatt tatcaaagaa acctcatcaa agaactctct
     5461 tttaccgccg tcggcatttt cgtcgtcctc ttggcggtat tggtctccac gcaggcaatc
     5521 aacctgctcg gccgtgccgc cgacgggcgt gtcgccatcg atgccgtgtt ggcattggtc
     5581 ggcttctggg tcatcggtat gacgccgctt ttgctggtgt tgaccgcatt tatcagtacg
     5641 ttgaccgtgt tgacccgcta ctggcgcgac agcgaaatgt cggtctggct atcctgcgga
     5701 ttggcattga aacaatggat acgcccggtg atgcagtttg ccgtgccgtt tgccgttttg
     5761 gttgccgtca tgcagctttg ggtgataccg tgggcagagc tacgcagccg cgaatacgct
     5821 gaaatcctga agcagaagca ggaattgtct ttggtggagg caggcgagtt caacagtttg
     5881 ggcaagcgca acggcagggt ttattttgtc gaaaccttcg ataccgaatc cggcatcatg
     5941 aaaaacctgt tcctgcgcga acaggacaaa aacggcggcg acaacatcat cttcgccaaa
     6001 gaaggtaact tctcgctgaa cgacaacaaa cgcacgctcg aattgcgcca cggctaccgt
     6061 tacagcggca cgcccggacg cgccgactac aatcaggttt ccttccaaaa actcaacctg
     6121 attatcagca ccacgcccaa actcatcgac cccgtttccc accgccgtac cattccgacc
     6181 gcccaactga ttggcagcag caacccgcaa catcaggcgg aattgatgtg gcgcatctcg
     6241 ctgaccgtca gcgttttgtt gttgtgtctg cttgccgtgc cgctttccta tttcaacccg
     6301 cgcagcggac atacctacaa tatcttgatt gccatcggtt tgtttttaat ttaccaaaac
     6361 gggctgaccc tgctttttga agccgtggaa gacggcaaaa tccatttttg gctcggactg
     6421 ctgcctatgc acattatcat gtttgccgtt gcactcatcc tgttgcgcgt ccgcagtatg
     6481 cccagccagc ccttctggca ggcggttggc aaaagtctga cattgaaagg cggaaaatga
     6541 acctgatttc acgttacatc atccgtcaaa tggcggttat ggcggtttac gcgctccttg
     6601 ccttcctcgc tttgtacagc ttttttgaaa tcctgtacga aaccggcaac ctcggcaaag
     6661 gcagttacgg catatgggaa atgctgggct acaccgccct caaaatgccc gcccgcgcct
     6721 acgaactgat tcccctcgcc gtccttatcg gcggactggt ctccctcagc cagcttgccg
     6781 ccggcagcga actgaccgtc atcaaagcca gcggcatgag caccaaaaag ctgctgttga
     6841 ttctgtcgca gttcggtttt atttttgcta ttgccaccgt cgcgctcggc gaatgggttg
     6901 cgcccacact gagccaaaaa gccgaaaaca tcaaagccgc cgccatcaac ggcaaaatca
     6961 gcaccggcaa taccggcctt tggctgaaag aaaaaaacag cattatcaat gtgcgcgaaa
     7021 tgttgcccga ccatacgctt ttgggcatca aaatttgggc gcgcaacgat aaaaacgaat
     7081 tggcagaggc agtggaagcc gattccgccg ttttgaacag cgacggcagt tggcagttga
     7141 aaaacatccg ccgcagcacg cttggcgaag acaaagtcga ggtctctatt gcggctgaag
     7201 aaaactggcc gatttccgtc aaacgcaacc tgatggacgt attgctcgtc aaacccgacc
     7261 aaatgtccgt cggcgaactg accacctaca tccgccacct ccaaaacaac agccaaaaca
     7321 cccgaatcta cgccatcgca tggtggcgca aattggttta ccccgccgca gcctgggtga
     7381 tggcgctcgt cgcctttgcc tttaccccgc aaaccacccg ccacggcaat atgggcttaa
     7441 aactcttcgg cggcatctgt ctcggattgc tgttccacct tgccggacgg ctcttcgggt
     7501 ttaccagcca actctacggc atcccgccct tcctcgccgg cgcactacct accatagcct
     7561 tcgccttgct cgccgtttgg ctgatacgca aacaggaaaa acgctaaacc aatgccgtct
     7621 gccccccttc ggacggcatt ggtttttatt atcatattcc cgtagacaga tagccgttcc
     7681 ctattatagt ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactattgta
     7741 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaaataca ttacctgtca taacagttcc
     7801 atttttgtta aaactagtct atgatagcgg tacaaatatt gtttacaata tttaacgcaa
     7861 atcatttgca acccgacaaa agaaaaacag aaaaaggaac aaagagatgt tagaagccta
     7921 tcgtaaagcc gccgccgagc gcgccgccct tggtattccc gcccttcctc tgactgctca
     7981 gcaaaccgcc gatttggttg agctgctgaa aagcccgccc gcaggcgaag gcgagttctt
     8041 ggtcgaactg cttgcccacc gtgttccgcc cggtgtggac gatgccgcca aagtcaaagc
     8101 ctcattcctg gctgccgttg ccgaaggcag cgcgtccagc ccgctgatct cccccgaata
     8161 tgcgaccgaa ctcttaggta cgatgctcgg cggttacaat atccacgcct taatcgaact
     8221 cttggacgac gacaaactcg cgcccattgc cgccaaaggc ttgaaacata cgcttctgat
     8281 gttcgattcc ttccacgacg ttcaggaaaa agccgaaaaa ggcaacaaat acgcgcaaga
     8341 agttttgcaa tcttgggcag atgccgaatg gttcgcctca cgcgccaaag ttcccgaaaa
     8401 aattaccgtt accgttttca aagttgacgg cgaaaccaat acagacgacc tctcccccgc
     8461 gcccgacgcg tggagtcgtc ccgatattcc gctgcacgcg ctggccatgc tgaaaaaccc
     8521 gcgcgacggc atcacgcccg acaaaccggg cgaagtcggt ccgattaaat tgttggaaga
     8581 actcaaagcc aaaggccatc cggttgctta cgtcggcgac gtggtcggta ctggttcttc
     8641 acgcaaatcc gcgaccaact ccgtcatttg gcataccggc gaagacattc cgttcgtgcc
     8701 gaacaaacgc ttcggcggcg tatgtttggg cggcaaaatc gcgccgattt tcttcaatac
     8761 ccaagaagat tccggcgcgc tgccgattga agtcgatgta tctgctctaa aaatgggtga
     8821 tgtcgtcgat atcctgcctt atgaaggcaa aatcgtgaaa aacggcgaga ctgttgccga
     8881 gtttgaattg aaatcacaag tattgctgga cgaagtgcaa gccggcggcc gtatcaacct
     8941 gattatcggc cgaggtctga ccgccaaagc gcgcgaagcc ctgaaactgc ctgcctctac
     9001 tgcattccgc ctgccgcaag cgcctgccga aagcaaagcc ggtttcacct tggcgcaaaa
     9061 aatggtcggc cgcgcctgcg gtctgcccga aggacaaggc gtgcgtccgg gtacttactg
     9121 cgaaccgcgt atgacgacgg tcggctcgca agacacgacc ggcccgatga cccgcgacga
     9181 gttgaaagac ttggcttgtt tgggcttctc cgccgatatg gtgatgcagt ctttctgcca
     9241 caccgccgcc tatccgaaac ctgtcgatgt aaaaacccat aaagaactgc ccgcctttat
     9301 ttccacccgt ggcggcgtgt cactgcgtcc gggcgacggc gtcatccact cgtggctcaa
     9361 ccgcctgctg ctgcccgata ccgtcggcac cggcggcgac agccataccc gtttccccat
     9421 cggtatttcc ttccccgccg gctccggctt ggttgccttt gccgccgcaa cgggcgtaat
     9481 gccgctcgat atgcctgagt ctgtattggt acgcttcagc ggcaagctgc aaccgggcgt
     9541 aaccctgcgc gatttggtga acgccatccc gctgtacgca attaaacaag gtttgctgac
     9601 cgttgccaaa gccggtaaga aaaacatctt ctctggccgc atcctcgaaa tcgaaggcct
     9661 gcccgatttg aaagtggaac aagcctttga attgaccgac gcatccgccg aacgctccgc
     9721 cgccggctgt accgtgaagc tcaacaaaga gccgattatc gagtacatga aatccaacgt
     9781 cgtgttgatg aaaaacatga ttgccaacgg ctatcaagac ccgcgcactt tggaacgccg
     9841 catcaaagct atggaaaaat ggctggcaaa tcccgagttg ctcgaagcgg ataaagatgc
     9901 cgaatacgcc gccgtgattg aaatcaacat ggacgacatc aaagagccga ttatcgcctg
     9961 cccgaacgac ccggacgacg tgtgcttcat gtccgaacgc tccggcacca aaatcgacga
    10021 agtattcatc ggttcgtgta tgaccaacat cggccacttc cgcgccgcct ccaaactttt
    10081 ggaaggcaag gcagacaccc ccgtccgcct gtggattgcg ccgccgacca aaatggacgc
    10141 gaaacaattg tccgacgaag gacactacgg cgtactcgga cgtgccggcg cgcgtatgga
    10201 aatgccgggt tgctccttat gtatgggtaa tcaggcgcaa gtacgcgaag gtgcgaccgt
    10261 tatgtccacc tccacccgca acttcccgaa ccgtttgggt aaaaacacct ttgtttacct
    10321 cggttcggcg gaattggcag cgatttgctc caaactgggt aaaatcccga ccgttgaaga
    10381 atatcaagcc aatatcggca tcatcaacga acagggcgat aaaatctacc gctatatgaa
    10441 cttcaacgaa atcgacagct acaacgaagt agccgagacc gtgaacgttt aatccccgtc
    10501 atccgtatga agtaagggat tgaccgcaat gccgtctgaa caaccttcag acggcattgc
    10561 aacattccgc taacccttct ttccgcaaac gctgcaaata cggcgttcac gcccccacat
    10621 aaaggaaacg acagtgaacc tgaaaaaccg ccattttctg aaacttttag acttcacgcc
    10681 ggaagaaatc accgcctacc tcgaccttgc cgccgaattg aaagccgcca aaaaagcagg
    10741 gcgcgagatt cagcggatga aagggaaaaa catcgccctg atttttgaaa aaacctctac
    10801 tcggacgcgc tgcgcgtttg aagtcgccgc gcgcgatcaa ggcgcgggag tgacttattt
    10861 agagccgtcc gccagccaaa tcgggcataa ggaaagcatc aaagacaccg cccgcgtgtt
    10921 gggcaggatg tacgatgcca tcgaatatcg cggtttcggt caggaagttg ttgaagaatt
    10981 ggcgaaatac gcgggcgtac ccgtgttcaa cgggctgacc aacgagttcc atcccacaca
    11041 aatgcttgcc gacgcactga ctatgcgcga acacagcggc aaacctttga accaaaccgc
    11101 gtttgcctac gtcggcgacg cgcgttacaa catgggcaat tccctgctga ttttaggggc
    11161 aaaattgggg atggacgtgc gtatcggcgc accgcaaagc ctgtggccgt ctgaaggcat
    11221 tattgccgcc gcacacgccg ccgccaaaga aaccggcgca aaaattaccc tgaccgaaaa
    11281 cgcgcatgaa gccgtgaaga atgttgattt tattcatacc gatgtgtggg tcagcatggg
    11341 cgagccgaaa gaagtctggc aggaacgcat cgatttgctg aaagattacc gcgttacgcc
    11401 cgaactgatg gcggcatcgg gcaatccgca agtcaaattc atgcactgcc tgcccgcctt
    11461 ccacaaccgc gaaaccaaag tcggcgaatg gatttacgaa accttcgggc tgaacggtgt
    11521 ggaagttaca gaagaaatat tcgaaagccc cgccagcatc gtgttcgatc aggcggaaaa
    11581 ccgtatgcac acgattaaag cggtaatggt cgcggctctg ggcgactgac agaactgtgc
    11641 ctgtttaaat tcatccgcaa cacagatacc gtctgaacac gatgttcaga cggtatcca
//
LOCUS       DAXHSJ010000050        11426 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000050 DAXHSJ010000000
VERSION     DAXHSJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.050"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(351..1136)
                     /locus_tag="ACFM8U_000980"
     CDS             complement(351..1136)
                     /locus_tag="ACFM8U_000980"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC6334237.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSICCGWWSLPFWVFTV
                     FALQFFRDPAREIPLNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QTESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            1291..4137
                     /gene="uvrA"
                     /locus_tag="ACFM8U_000981"
     CDS             1291..4137
                     /gene="uvrA"
                     /locus_tag="ACFM8U_000981"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC6334238.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEGTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYSFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIDRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIASGSPEEVAKVKGSYTGKYLKNIL"
     gene            complement(4169..4339)
                     /locus_tag="ACFM8U_000982"
     CDS             complement(4169..4339)
                     /locus_tag="ACFM8U_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334239.1"
                     /translation="MFLGIFIALSGILTLLEMFNVISSDMKWGMPLMFTCLGFSIIFD
                     SFKAKQNRKQKE"
     gene            complement(4354..4539)
                     /locus_tag="ACFM8U_000983"
     CDS             complement(4354..4539)
                     /locus_tag="ACFM8U_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334240.1"
                     /translation="MTNKIKPGQNTGNNGGIYQEIGSRGGRKDNYATIKDNQKAPPTQ
                     NPGSSWIQIKRTPDSSR"
     gene            complement(4555..4824)
                     /locus_tag="ACFM8U_000984"
     CDS             complement(4555..4824)
                     /locus_tag="ACFM8U_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334241.1"
                     /translation="MKIKFAHLRHPAVGGGYINFAVFDAKSTNGNHSVVLKELTTEAR
                     RNGLKIDQSALIFEQHGRTQFYGDKNLVDFLSANWYPQWTHTLTI"
     gene            4980..5903
                     /locus_tag="ACFM8U_000985"
     CDS             4980..5903
                     /locus_tag="ACFM8U_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HFC6334242.1"
                     /translation="MKTIAPSHIKPLMPELNQSQRNRIIFIDFYLCFLGKINRKDLMQ
                     AFQISPAVATRDFALYCSSFSKNLRFDTKSKFYLSTEDFEPQFPHSFENAIKILNGSL
                     IFDIDSQHQPLLPFDLPPVLINPDVLIFSKISKALSLKKPLKISYHSISSGLSEKVIV
                     PLALVNDGFRWHLRAFDRTKNRFADFVLTRIIQANILLAENIEENETINNDIDWNRIV
                     KIELVPHPNHDEAKKVIEMDFGMTNGKLELALRASNIGYVLQQWRVDCSSDHHIDDPA
                     FRLWLLDNLVLYGINNAEHLAPAFDKKRSKP"
     gene            complement(6130..6396)
                     /locus_tag="ACFM8U_000986"
     CDS             complement(6130..6396)
                     /locus_tag="ACFM8U_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334243.1"
                     /translation="MKRQTVNQATACVRTAHTLHVDFEVPCNSQVGCVAQPRTRCWVC
                     RLRLAAAETPEEKAKDAAEEAKEEAKDAAEDAKEAAEDAKEEAK"
     gene            6530..6673
                     /locus_tag="ACFM8U_000987"
     CDS             6530..6673
                     /locus_tag="ACFM8U_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334244.1"
                     /translation="MARYRRNFIAGGTFFFTVKLADPKSRLLVEHIDFAGGLYGCAKT
                     IFL"
     gene            6645..6974
                     /locus_tag="ACFM8U_000988"
     CDS             6645..6974
                     /locus_tag="ACFM8U_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6334245.1"
                     /translation="MDVQKQYSFETVAVCVLPNHIHAIWTLPPDDADYSLRWRLIKTK
                     FSAHFPHAENLSASKQRRHERGIWQRCFYEHTVRDETDLQHRADYIHSNPIKQRMSRA
                     KTESRKK"
     gene            complement(7211..8101)
                     /gene="sucD"
                     /locus_tag="ACFM8U_000989"
     CDS             complement(7211..8101)
                     /gene="sucD"
                     /locus_tag="ACFM8U_000989"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC6334246.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKTKGLA"
     gene            complement(8112..9323)
                     /gene="sucC"
                     /locus_tag="ACFM8U_000990"
     CDS             complement(8112..9323)
                     /gene="sucC"
                     /locus_tag="ACFM8U_000990"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC6334247.1"
                     /translation="MEADSGHSTNLKEKSMNLHEYQAKELLASYGLPVQGGILAHNGE
                     EAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTD
                     ANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKI
                     FKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLA
                     VRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIG
                     CMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINI
                     FGGIVRCDMIAEAIVAAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLND
                     AAEKIVAAVNA"
     gene            complement(9377..9664)
                     /locus_tag="ACFM8U_000991"
     CDS             complement(9377..9664)
                     /locus_tag="ACFM8U_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334248.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(9789..11222)
                     /gene="lpdA"
                     /locus_tag="ACFM8U_000992"
     CDS             complement(9789..11222)
                     /gene="lpdA"
                     /locus_tag="ACFM8U_000992"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6334249.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
BASE COUNT         2860 a         2998 c         2617 g         2951 t
ORIGIN      
        1 gtaactaatg agttgtcgtt cccacgcagg cgggaatccg gtctcttcgg tttcagtcgt
       61 ttccgataaa tcgtcgtaac gttaagttcc cggattccca ctttcgtggg aatgacgcgg
      121 aaaggtttct gttttttaag tgttaggtaa ctaataagtc gtcattcccg cgcaggcggg
      181 aatccagtcc gtccggtttc agtcgtttcc gataaatcgt cgtaacgtta agtttctaga
      241 ttcccacttt cgtgggaatg acggtgtgaa cattagcaaa aattcaaaaa accaaacttc
      301 ctcattcgtg tgaatgatgg tactgatatt ttctatttca gtccgcaaaa ttaatctttc
      361 aacacatctt gcacagccgc ctgaatctta gccgccgctg cctcgatttg ccgttgttcg
      421 gcagatgggt ttgccgctgt ttcaaccgga gcagtttgta aagcaggctc agattcagtt
      481 tgcggcgcag tcagcggcaa acgcgccaat acagtgctga cgccggttac tttatcgcca
      541 atcgccactt gcgcctgcgc atcgacaggc agatacatat ccacgcgcga accgaagcgg
      601 ataaagccat aacgttcgcc gcgggacagt ttcgcacctg cttgggtgta gcacaaaata
      661 cggcgcgcca ccaaaccggc cacttgaaca aaagtaattt cacgacctga agccgtagtc
      721 gccaacaccg cattacgttc gttttccgtg ctggctttgt ccaaatccgc attcacgaat
      781 ttgcctttgt tatagaccac tttcgttacc gtacaatcgg caggcgattt ttgcgaatgc
      841 acgttgaaca cgttcataaa aatactgatt ttcaaagcat cgacatcacg atacggatcg
      901 cgtgcgcgtt cgaccaccac gatacggccg tcaaccgggc tcaacaccgc ttcaggattt
      961 agcggaatct cacgcgcagg gtcgcggaaa aactgcaatg caaatacggt aaacacccaa
     1021 aacggcaaag accaccagcc gcaacatatc gacaccagca ggctcaaagc caaaccgccg
     1081 ccaataatcg gccagccctc acgggcgata atcgggtggg ggtaaagacg gttcatttgt
     1141 catccttttg ttcaagcggg ttaaaattgc cgacattata gcggaatctg ccgtactccg
     1201 ctggcaaatg ccgtctgaac gcggcttcag acggcatccg agaaagtgct acaataccgg
     1261 ttttccttta tttttcaggc ggcatccatt atgtgcaacc atcatcccca acattcgcac
     1321 gacaacgaca ccatccgcat ccgcggcgcg cgcacgcata atttgaaaaa catcgattta
     1381 gacattccgc gccacaaact cgtggtggta acaggattgt cgggcagcgg caagtcgtcg
     1441 ctggcgtttg acacgctgta tgccgaaggg caacggcgtt atgtcgagag cctttccgcc
     1501 tatgcgcggc agtttttgca gatgatggac aaacccgatg tcgatttgat tgaaggtctg
     1561 tcgcctgcga tttccatcga gcagaaatcc accagccaca acccgcgttc caccgtcggc
     1621 acggtaacgg aaatccacga ctacctgcgc cttttgtacg cccgcgtcgg cacgccgtat
     1681 tgccccgaac acaatctgcc gttgtcgagc caaaccgtat cgcagatggt cgatgccgtc
     1741 ttaaaactgc cggaaggcac gcgcgtgatg attctggcgc cggcggtgcg cgagcgtaag
     1801 ggcgagtttg tcgatttctt tgccgacttg caggcacagg gttttgcgcg cgtgcgcgtg
     1861 gacggcgaag tctatcagtt ggacgaagtg ccgaagctgg aaaaaaacat caaacacaac
     1921 atcgacgtgg tcatcgaccg cgtgaaagtg aaggcggaca tcaagcagcg gctggcggaa
     1981 agttttgaaa ccgcgctgcg ccacggcaac gagcgcgcgc tggcgatgga gatggacagc
     2041 ggcgaagaac attggttttc cgcgcgtttt gcctgccccg tgtgttcgta cagcctgccc
     2101 gaattggagc cgcgcctctt ttcgttcaat aatccgatgg gctcctgccc gacttgcgac
     2161 ggcttgggca acaccaattt cttcgacccc gaaaaagtgg tcgcccaccc cgaattgtct
     2221 ctggcttcgg gcgcgattga cggctgggac aagcgcaacc agttctattt ccaaatgatt
     2281 cagtcgctgg cacggcatta cagttttgat gtgcaggctg cttgggaaac cctacctgca
     2341 aaaatcaaaa aagtcgtgtt gcacggctcg ggcaaggaag tcattgattt cacctaccta
     2401 tccgaacgcg gcaccacctt caaccgcagc cacgccttcg aaggcatcat tcccaatctc
     2461 gaacgccgct accgcgaaac cgacagcgaa accgtgcgcg aaaaactgcg cgaataccaa
     2521 aaccaccgcg cttgcccgag ctgcggcggc gcacgtttgc gcaaagaagc ccgctacgtt
     2581 tacgtcggcg gcgagccgtt gcacgaagtt tccgcctggc cgctgaccaa aacccaccga
     2641 ttctttgaaa cgctggattt ggacggcaac aaaaaacaaa tcgccgaaaa aatcctcaaa
     2701 gaaatcaccg aacgtctcgg ctttttgatt aatgtcgggc tggattacct gaatctctcc
     2761 cgttccgccg aaaccctttc cggcggcgaa gcccagcgca tccgcctcgc cagccaaatc
     2821 ggcagcggcc tgaccggcgt gatgtacgtt ttggacgaac cctccatcgg cctgcaccag
     2881 cgcgacaacg accgcctgct tgccaccctc aaacgcctgc gcgatttggg caacagcgtc
     2941 atcgtggtcg aacacgacga agacgccatc cgcgaagccg atttcgtcgt cgatatgggc
     3001 ccgggcgcag gcgaacacgg cggcaacgta ttgattgccg acacgcccga aaacgtcgcc
     3061 aaatgcgaaa actccgttac cggacaatac ctcagcggca aaaaatccat tgccgtgccg
     3121 tctgaacgca cgcccgtcaa tcccgaccga atgctcgtcc tcaaaggcgc gcgcggcaac
     3181 aacctcaaaa acgttaccct tgagctgccg ctcggtttga ttacctgcat caccggcgta
     3241 tccggcagcg gcaaatccac cctgattaac gacaccctcg ccaaaatcac tgcccgcgaa
     3301 ctcaaccgcg cccaagaaga acccgcccca tacgacgaca tccgcggcct cgaacacctc
     3361 gacaaagtca tcaacgtcga ccaatccccc atcggtcgca ccccgcgctc caaccccgcc
     3421 acctacaccg gcctgttcac ccccatccgc gaactcttcg ccggcgtacc cctctcgcgc
     3481 gaacgcggct acaacgtcgg cagattctcc ttcaacgtca aaggcggccg ctgcgaagcc
     3541 tgccaaggcg acggcgtgat taaagtcgaa atgcacttcc tgcccgacgt gtacgtcccc
     3601 tgcgaagtct gccacggcaa acgctacaac cgcgaaaccc tcgaaatcca atacaaaggc
     3661 aaaaacatca gtcaagtcct cgacatgacc gtcgaagaag cccgcgaatt cttcgacgcc
     3721 gtccccaccg tatcgcgcaa actgcaaacc ttgatggacg taggcttagg ctacatccgt
     3781 ctcggccaat ccgccaccac cctctccggc ggcgaagccc agcgcgtcaa actcgccttg
     3841 gaactctcca aacgcgacac cggcagaacg ctctacatcc tcgacgaacc caccaccggc
     3901 ctacacttcg ccgacatcgc cctgctgctg gaagtcatag accgtctgaa aggcaaaggc
     3961 aactcgatag tgattatcga gcataatctg gatgtgatta aaaccgccga ttggattgtg
     4021 gacttagggc cagaaggcgg cgacggcgga gggaggatta ttgcttcagg tagcccggag
     4081 gaagtggcga aggttaaggg gagttatact gggaagtatt tgaagaatat actttaaaaa
     4141 acgggatggt ttaaaccatc ccgttttttt attccttctg ttttcgattt tgtttggctt
     4201 tgaagctatc aaaaataatg ctaaatccca aacaggtgaa cattaaaggc atcccccatt
     4261 tcatatccga agaaataaca ttaaacattt ctaataatgt aagaatgcct gataaagcta
     4321 taaaaatacc taaaaacatt agaactatcc ttattatctg gagctatctg gagttctttt
     4381 aatttgaatc catgagcttc ctggattttg agtgggtggt gctttttgat tatctttaat
     4441 ggttgcgtag ttatcttttc gaccacctcg tgaaccaatt tcttgataaa ttccaccgtt
     4501 atttcctgta ttttgaccag gtttgatttt gttcgtcatt atcatctcca taatttaaat
     4561 agttaaagta tgagtccatt gcggatacca atttgcagat aaaaaatcta ctagattttt
     4621 gtctccatag aattgtgtac gcccatgttg ttcaaatatt aacgcagatt ggtcaatttt
     4681 taatccattt ctacgagctt cagtggtcaa ttccttcagt acgacactat ggttaccatt
     4741 agttgattta gcatcaaaaa cagcaaagtt aatatatcct ccacctacgg ctggatgtct
     4801 taagtgagca aattttattt tcataaataa atcctataaa aaattacata acagaaattt
     4861 taagtcagag agctactatg aagtagcttt ttgcattata tgcactcttt ttgattacgt
     4921 caagcaattt ttttgaattc atattgccat gaaaaagatt gatggaatat aatgcaatta
     4981 tgaaaacaat cgctccttcc catataaagc cccttatgcc agagttgaac caatctcaac
     5041 gcaataggat tatatttatt gatttctatc tatgctttct aggaaagata aatagaaaag
     5101 atttgatgca ggcatttcaa atttccccag ctgttgctac tagggatttt gcgctatatt
     5161 gctctagttt ttcgaaaaat ctccgttttg ataccaaaag taaattttac ctctctactg
     5221 aagactttga acctcaattc ccccattcat ttgaaaatgc aattaaaatt cttaatggca
     5281 gtttaatatt tgatattgat tcccaacatc aacctcttct tccgtttgat ttacctcctg
     5341 ttttaataaa tcctgatgtc ttaatctttt ctaaaatatc gaaagcatta tccttaaaaa
     5401 aacctctaaa aatctcatat cattcaattt catctggatt atctgagaaa gttattgtgc
     5461 ctcttgctct agttaatgac ggatttcgat ggcatttacg ggcattcgat agaacaaaaa
     5521 accgatttgc agattttgtc ctaacacgaa taattcaagc aaatatttta ttagctgaaa
     5581 atattgaaga aaatgaaacg attaacaatg atattgattg gaatagaatt gttaaaatcg
     5641 agttggttcc ccatcctaat catgatgaag ctaaaaaagt gattgaaatg gactttggaa
     5701 tgacaaatgg aaaattagaa ttggcgctta gagcttcaaa tatcggatat gttttacagc
     5761 aatggcgagt cgattgttcg tcagatcatc atatcgatga tcctgctttt agattatggc
     5821 tgttagataa tttagtttta tacggcatca acaatgctga acacttagcg cctgcctttg
     5881 ataaaaaacg tagcaagccg tagcctacat atccaaccga ccgcgtgcgt ggctgcgcca
     5941 cacacgcggt tgtttgattg atggctttct gttgtaaatt gaacttagtt aaaactaaca
     6001 ctttcagacg gcatgaaaaa tggccgtctg aaaactgaaa aagctttatc taaaacaact
     6061 accctaactc acaaaacaaa aaacggattc ccaatcattg gaatccgttt ttctgtaaag
     6121 ctatccgaat tatttagctt cttctttagc gtcttcagca gcttctttgg catcttccgc
     6181 tgcgtctttt gcttcttctt tagcctcttc agcggcgtct ttggcttttt cttcaggagt
     6241 ttctgcagca gcaagccgta gcctgcatac ccaacaccgc gtgcgtggct gcgccacaca
     6301 ccctacctgt gaattacacg gaacttcaaa atcaacgtgt agggtgtgtg cggtacgcac
     6361 gcacgcggtt gcttgattga cggtttgccg tttcaacttt taacttcgtt gaagctagcg
     6421 ctttcagacg gcctccaatg tcgtctgaaa gcgctagctt caacgaagtt aaaaacctta
     6481 aacaacaagc cattccttct ccaacgctca aaccaaaaac aaaaatccta tggcgcgtta
     6541 tcgcagaaac ttcattgccg gcggcacatt ctttttcacc gtcaaactcg ccgacccgaa
     6601 atcgcgcctg cttgtcgaac atatcgactt cgcgggcggc ttatatggat gtgcaaaaac
     6661 aatattcctt tgaaaccgtc gccgtatgcg tgctgccgaa ccatatccac gccatttgga
     6721 cgctgccacc cgacgatgcg gattattccc tgcgctggcg gctgattaaa accaaattct
     6781 ccgcacattt ccctcatgcc gaaaacctgt ctgccagcaa acagcggcgg cacgaacgcg
     6841 gtatttggca acggtgtttt tacgaacaca ccgtgcgcga tgaaacggat ttgcagcatc
     6901 gtgcggacta catccattcc aatcctatca aacaaagaat gtcgcgtgct aaaacagaat
     6961 cccgcaaaaa ataatattag tcttttcaaa tgcgatactt atccattccc ggacttcaaa
     7021 caaggcgtgt gcaacctgac ttacgggttt gctcctgttt gaccgataaa tattctgact
     7081 gcctcaaagg gcaaaaaata atgtgggtat gttgaggctg cctctttttg aaatgacaaa
     7141 caaaatgccg tctgaaacct gatttccggt ttcagacggc atttgatgtt caatcagttg
     7201 tcaaacctga ttatgccaaa ccttttgttt tcaacacttc cagcatggta gtgcccaact
     7261 cggcagggct gcgggtgtaa gcgataccgg ctttttcgaa agcggcgaat ttttcctcgg
     7321 cagtaccttt accgccggaa ataatcgcac cggcgtggcc catacgtttg cctttaggag
     7381 cggtaacacc agcaatgtaa cctacaacag gtttggttac gttggattgg atgtattcgg
     7441 ctgcttcttc ttccgcagta ccgccgattt caccgatcat gatgatggcg tcggtatctg
     7501 ggtcttcttg gaaaagtttc agtgcgtcga tttggttcat accgggaatc gggtcgccgc
     7561 cgataccgat acaggttgat tgacccaagc ccagtttggt ggtttgtgcc acggcttcgt
     7621 aagtcaatgt accggaacgg gaaataatac cgatgcggcc cggagtgtgg atgtggcccg
     7681 gcataatacc gattttgcac tcgcctgggg taatcacgcc cgggcagtta gggccgacca
     7741 agcgggtacc gttgccgttg gtttccaagt aacgtttggc tttgagcatg tccagagtcg
     7801 gcacgccttc ggtaatcaca accaccaagc ctacgccgga atcaacggct tcaacgatag
     7861 aatccaacac aaacggagcg ggaacgtaaa ttacagatgc atccgcgccg gtttctttaa
     7921 cggcttcttt catggtgttg aacacgggca ggttcaggtg ggtttgaccg cctttgcccg
     7981 gggtcacgcc gccgacaact ttagtgccgt aagccagagc ttgttcggag tggaaagtac
     8041 cgtttttacc ggtgaaacct tgaaccaata ctttggtgtc tttattaatc aatacgctca
     8101 ttcttttctc cttaggcgtt tacggctgcg acaatttttt cggctgcgtc attcaggccg
     8161 tctgcagaag tcagtttcag acctgattcg ttcaggattt tcgcgccgag ttcggcgttg
     8221 ttgccttcca aacgaacaac gacaggaacg tcgacgttga tttctttaac ggctgccacg
     8281 atggcttccg caatcatgtc gcaacgtacg ataccgccga agatgttgat caatacgcct
     8341 tgaacggatt tgtcttccag aatcagtttg aacgcttcaa ccacgcggtc tttggttgcg
     8401 ccgccgccaa cgtccaagaa gttggcaggt tggccgcctt tcagtttgat gatgtccata
     8461 gtggccatcg ccaaaccggc accgttcacc atacagccga tgttgccttc cagggcaaca
     8521 tagttcaggt caaattcaga agctttcaac tcgcgttcgt tttcttgaga tttgtcgcgc
     8581 aattcggcga ttttcggcag gcggtagagc gcgttgctgt cgatgccgat tttgccgtcc
     8641 acgcaggcga gcgcgccgtt ttcgcgaact gccagcgggt ttacttcaaa cagggcgaag
     8701 tcattttcga caaacgcttt gtacgcaccg gtcatcagtt tgacgaactc gttgatttgt
     8761 ttgtctttca agcccagttg gaacgcaact tcgcgagctt ggcaaggttg caggccgacc
     8821 agcggatcaa cggttacttt gaagattttt tcaggagttt cggcagcaac tttttcgatt
     8881 tccacgccgc cttcggtaga ggccatgaat gtaatgcggc gggtagaacg gtcaaccact
     8941 gcgcccaagt acagctcggt ttgaaccgga tacatgtctt cgcaaaccaa aacactgttg
     9001 acaggttggc cgttggcatc ggtttggtaa gttaccaagt tggtgccaat caggctttca
     9061 gccacttctt tagcttcttc gcggctttta acgactttta cgccgcccgc tttaccgcgg
     9121 ccgccggcgt gtacttgtgc tttgacaaca gcgaatttgc cgcccaattt gtcgtaagct
     9181 gcagcggctt cttcgccgtt gtgtgccaaa ataccgcctt gtacgggcaa accgtagcta
     9241 gccagcagtt ctttagcctg atactcgtgt aaattcatgg atttctcctt tagattagtg
     9301 gaatgccccg agtctgcctc caaatggcaa gcccgtgcgt ttcagactac ctttcggcgg
     9361 tcggaaaatc gtcgtttcac ttgctgaaag gcaaccacat ccgcgccagc agatacatgg
     9421 ctgcaccgcc tcccccgaga ataaaaaaga cgatgccttt tttacgcgaa ttcggacaga
     9481 gcagataaac ggcaaggctg aaactgataa acaacaatgc cacgcgccaa accaattctt
     9541 tatctttgaa acggttgagc gtataagttt cggtcatcat cacatacatc tgccacaatg
     9601 cggccataca catgctgatg atgcccatcg gtataaagaa aataccgact atttctttgt
     9661 tcataacgaa tgtaaatgct gtttgtttgt ctgacggaat tataaatcag aaaacggagc
     9721 tctcggaaca ttcaatttgt cgaaggcatt aaggtcgtct gaaaaatttc agacgacctt
     9781 aatgtctatc aaccgtgcaa agcgcgtttg tcggccgcca atgcagcttc gtgaaccact
     9841 tcggacaagg ttgggtgggc atggataatg cgggcgatgt cttcgctgct ggcgaagaat
     9901 tcgagcgcag tcacgccttc ggtaaccaat tcgctgacaa ccggaccaat catgtgtacg
     9961 cccaagatgc ggtcggtttt ggcgtctgcc aacactttaa ccgtaccttt ggctttgccc
    10021 attgccaatg cgcgaccatt cgcaccaaaa cctgaagtac cttttttgta ctccacgcct
    10081 tcggctttaa gctgttcttc ggttttaccc acccaagcga tttcaggatc ggtgtaaatc
    10141 acaaacggta cgttgttgaa atcgatatgc ggtttttgac cggcaatgcg ctcggcaacg
    10201 gcaacgcctt cgtcgctggc tttgtgtgcc aacatcgggc cgcgaaccac gtcgccgatt
    10261 gcccatacat taggcaggtt ggtgcggcat tcgccatcta ctttgataaa gccgcgctca
    10321 tctttttcca agccgacggc ttcagcgttc aggcctttgg tgtttggaat acggccgatg
    10381 gcaacgatca gtttgtcgaa tacttcggtt ttggcttcgc cggcagcagt ttcgtaagca
    10441 acggaaacgc ctttgccttc agatttgatg tcgccgattt tcacgcccaa ttcgatgctc
    10501 aaaccttgct ctttggtgaa gtatttgaag gcttctttgg cgatttgttg gtcggcggct
    10561 gccaggaagg tcggcgcagc ttcaagaatg gtaacttccg cacccacgcg gttccatacg
    10621 gaacccattt ccaaaccaat cacgccagaa ccgatcacgc cgagtttggc aggtacttcg
    10681 gtcaggttca atgcgccttc gttgtccaat acgttcacat tgtcgatagc gacttgtggc
    10741 agcggacgcg gtacggaacc ggtggctacg atgacgtgtt tggcttcgat aacggttttc
    10801 tcgcctttgt tatcgacttc gatttggtaa gcatcaccat ttttaccggc aaaggaagcc
    10861 gtaccgaaca ggctggttac tttatttttt tggaacagga atttcacacc gccggtcagt
    10921 ttggtcacga tggcatcttt gcgctcaatc attttggccg catcgaattt tacgtcgccg
    10981 acagtgatgc cgtgttcggc aaactcgtgt tgcgctgcgt ggaaatgttc gctggactgc
    11041 aacagggctt tggaagggat acagcctacg ttcaagcaag taccgcccaa tgcaggggca
    11101 ttgcctgctt tgttaacgcc tgcatcgaca caggcagttt tgaaacccag ttgtgcggca
    11161 cggatggcgg caacgtatcc gcccgggcct gcaccaatca ctactacatc atattgagac
    11221 atcgtgtcat cctttgcttt tagcagtatc tcaaaataaa aacgatactt ttcgtttctt
    11281 ttttattttg aatgactgcg ttttgatggt tttaagaaaa agccgtctga aagtccatat
    11341 ctgccgattc attcgaatca aatgtttcag acggcctcaa tcgttcgttg agcctattgt
    11401 agcacgatac tacattacgc tacatc
//
LOCUS       DAXHSJ010000051        11308 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000051 DAXHSJ010000000
VERSION     DAXHSJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.051"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(62..1207)
                     /gene="dapE"
                     /locus_tag="ACFM8U_000993"
     CDS             complement(62..1207)
                     /gene="dapE"
                     /locus_tag="ACFM8U_000993"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC6334250.1"
                     /translation="MTETQSLELAKALISRPSVTPDDQNCQKLLAERLHKIGFAAEAL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAENPDHQGSIALLITSDEEGDAQDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMLKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCLGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     ITKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILVRLLAGNAV"
     gene            complement(1307..1927)
                     /locus_tag="ACFM8U_000994"
     CDS             complement(1307..1927)
                     /locus_tag="ACFM8U_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HFC6334251.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQSEPDR
                     KLQNGEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGA"
     gene            complement(1961..2470)
                     /locus_tag="ACFM8U_000995"
     CDS             complement(1961..2470)
                     /locus_tag="ACFM8U_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6334252.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQLVLQTIRQITRYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHVSLHGN
                     WDAVAAEFAKLEADNAYIPDAEAFKRFVAGYDVHLAIEEPLFDMGKMFIPKEKLTEIG
                     EIMAARRRR"
     gene            complement(2565..3116)
                     /gene="azu"
                     /locus_tag="ACFM8U_000996"
     CDS             complement(2565..3116)
                     /gene="azu"
                     /locus_tag="ACFM8U_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC6334253.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(3397..3819)
                     /locus_tag="ACFM8U_000997"
     CDS             complement(3397..3819)
                     /locus_tag="ACFM8U_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC6334254.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     VCIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            3950..6700
                     /gene="secA"
                     /locus_tag="ACFM8U_000998"
     CDS             3950..6700
                     /gene="secA"
                     /locus_tag="ACFM8U_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC6334255.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            6846..8618
                     /gene="dnaG"
                     /locus_tag="ACFM8U_000999"
     CDS             6846..8618
                     /gene="dnaG"
                     /locus_tag="ACFM8U_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC6334256.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQETPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            8806..10734
                     /gene="rpoD"
                     /locus_tag="ACFM8U_001000"
     CDS             8806..10734
                     /gene="rpoD"
                     /locus_tag="ACFM8U_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC6334257.1"
                     /translation="MSRNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            10884..>11308
                     /locus_tag="ACFM8U_001001"
     CDS             10884..>11308
                     /locus_tag="ACFM8U_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6334258.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKLINRQLTEKGLKVEKASAAV"
BASE COUNT         2971 a         3172 c         2759 g         2406 t
ORIGIN      
        1 tcaaacccac gttaggtcta agcaaatggt cggacatccg tatccggcaa gcaagctgct
       61 ttcagacggc atttccagcc aacaaacgca ccaatatccc ctcatacacc gccgacagct
      121 tcggaatgtc gtttagccgc acgttttcgt tgatttggtg gatggtggcg ttggacggac
      181 ctaattcgat aagctctttc gtaatggctt taatgaagcg tccgtccgaa gttccgcctg
      241 tggtggacaa ttcggtctct acgccgcaga tttcggcaat ggcggcgcgt gccacgtcgg
      301 tcagtttgcc cgcgtgggtc aggaagggct gccccaaaca cgaccactgc aaatcgtatt
      361 gcacaccgtg tttgtccaaa atggcgtgga cgcgttgttt cagccctgct tcggtggact
      421 cggtggagaa gcggaaattg aatttgacat tcagctcgcc cggaatgacg ttggtcgcgc
      481 ctgtgccgcc gttgatattg gaaatttgaa agctggtcgg cgggaagtat tcgttgcctt
      541 cgtcccagac ttcctgtgtc agctctaaca aggcaggggc aaaagtgtgg acggggttga
      601 ttgccaaatg cggataggca atatgccctt gcttgccttt gacggtcagg ttgcctgaca
      661 gcgaaccgcg ccggccgttt ttcaacatat cgcccaattt atccacggca gtcggttcgc
      721 cgacgatgca gtaatcgatc aactcgccgc gcgctttcaa cacatcgacg acttttgttg
      781 tgccgtcctg cgcatcgccc tcttcgtccg aagtaatcag gagcgcaatg ctgccttgat
      841 gatcgggatt ttcggcaacg aaacgctcac aggcggtaac aaaacaggcg atgctggttt
      901 tcatgtccgc cgcgccgcgc ccgtataatc ttccgtcgcg ttcggtcggc tcgaacgggg
      961 gcgaatccca tttttcgaca gggcctgtcg gcacgacgtc agtatgccct gcaaaacaga
     1021 cgacgggagc tttcgtgccg cgtcgcaacc agatgttttt ggtatcgccg aaatggagtg
     1081 cctcagccgc aaaaccgatt ttgtgcaggc gttcggcaag gagtttttgg caattttggt
     1141 catccggcgt aacggacggg cgggaaatca acgctttggc gagttctagg gattgggttt
     1201 cggtcatttt tcggcttttc tgtttgagat gccgtctgaa cggagtttca gacggcattt
     1261 gatgttgaat ggaacgggct aacgatagcg aagcgggttc ttcaggtcaa gcaccatgac
     1321 acgcttcata aagcggcagc aaatcttcca ccgtttccgc tatccatttc gcgacatcct
     1381 gcctgcccaa atcgtcgcgt tcgatatgtt tgccgatgca gaagaagtct tcgccgtttt
     1441 gcaactttcg gtcgggttcg ctttgttgcg cgacggttcg gtagtcgtcg tattcgcttt
     1501 ccgcaccgtg ccacatatcg aaagaagcgt atttttcggt atcaaaatta tccaaccagc
     1561 ggttgtaatc aggcagcgcg atgggggaaa catcggcttt atagcagtgc caatccaagc
     1621 tgacgctcag acggcggcgg ttgagcagta tcgacaaaat cgctgcggaa tttttatatt
     1681 gttcgtattt gaagtaggca aagaaatggg cgcgaacctg ccagccgttg caccagcgtt
     1741 cgatgtgcgg cggcgcaaac ggcgcaccca attcggcggc aacctgctga atcagctgct
     1801 gccatatctg ccagttttcc ttgtagtcgg ctttgatttg cggaatgctt tcaggctggt
     1861 attttttaag ctgggaaaat tggaaaaacg ggatattgaa caaatcgcaa cttttcgggg
     1921 tcagcataat atatccttga gacgattgtt tcagacggca ttatctgcgc cggcgcgccg
     1981 ccatgatttc gccgatttcg gtcagtttct ctttggggat gaacatcttg cccatgtcga
     2041 acaagggttc ttcaatcgcc aaatgcacat catatcccgc cacaaagcgt ttgaacgctt
     2101 ccgcatcggg aatataggca ttgtctgctt cgagtttggc aaattcggca gccacggcat
     2161 cccagttgcc atgcaggctt acatgttggc gtaaaagctc gtccacgctt tcttgggctt
     2221 gcggcgcata ttgcagcagg agcgggaaaa agttttcttc ttcgtcctca tggtgcagcg
     2281 gcgcggcaac gttgaaatac cgggtgattt ggcggatggt ttgcaaaaca agctgattgc
     2341 agccgttttc ggcgatgtag tccgacagca tcgcgacttg gccgcaaaaa cggcgtactt
     2401 tgccgtggca ggcatacagc atttcaatcg gttcggcaaa ggtaacgctt ttggtttcaa
     2461 acggattcat gttttcgttc tcaacgggca cttttcaagc agtcatttta taataaaaca
     2521 gcctgcacaa agcaggctgt ccgtcttttg agactttaag cggattaatc gaccaaagtc
     2581 actttgccgt tcatcaaagc accgtgacct gggaaggtac aagcgaattt atattcgccg
     2641 tcggccaatt tagcaggatc cagagtcagg gaagcttctt cgccgccgcc gatcagtttg
     2701 gtatgggcaa caacgcgtgc atcatcaggt ttgacatagt cggtatcggc agcacctacg
     2761 ccgtctttaa atacgccgtc catgtcttca gctttggcaa tcacgagatt gtgacccatg
     2821 ctggctttgg gttgcgtacc ggtatgtttc agagtgatgg tgaactcttt acatgctttg
     2881 ctgacttgga tgtctttggt gttgaactgc atattgtcgt tggattcgac agttgccgca
     2941 cagttgccgg cagcaggggc ttcggcagca tctgcaggag cagcttcggc ggcaggtgct
     3001 tcggaagcgg gtgcttcagc agcaggagtt gcctcggcag caggcgcggc aggttcttga
     3061 gagcaggcag ccaaaccgat aacggcggca gaaatcagag ccagatacgc tttcataaca
     3121 aatctccaat cgataaaata atattcggtt ttacagaaat caaagtgcaa ccgccattaa
     3181 caaaaccttg aaaaagattc cgccgcgttg cacaaacaga tgtttcggag cggcattttg
     3241 ctacaaattt catttgaaat caaagcctgt ctgcaagttt acaatcgttt actttaaaag
     3301 gggcaatttt accccgaacc tatttcttta gtattagacc cattatcctt tacttcttaa
     3361 tattaacgga tgtttacaca aattcccgta tacattttat gcgccatgcc ttctaaccaa
     3421 gtttgccaat gcctccgcca attcgggatg ccgtttttcc aactttaccg ccgccgaacc
     3481 gaaactctcc agcgcagcct tactcaaatg cagggtattg gttttcggcg gtttttccgg
     3541 tttcgggacc agcctgaccg aaacagagcg tatcgaagcg tcaagccctg ccaactgcgg
     3601 caataccgac ggtgcaatca ttttcaagcg cgatgccgcc atattgtttg ccgccaaaag
     3661 gacaagcctg ccgtcttcga tacataccgt ctgaaaatgc gggtgcaggt tggcaggcag
     3721 cagttttttc acggcggcat ccaaccgccg ccactgtccc gcctgtttca aaagtccgga
     3781 aagcagcgcg tcccgcctgc ccaactgttc caaattcata aaacatacac ccaaaaagat
     3841 tgaaataccg caaacgcgcc tttatttcag acggcattag cactttgcac aaacgcttgt
     3901 gttaaaatcg cgttttcgcc cactattata tcaggcgcag gaattattca tgctgacaaa
     3961 cattgccaag aaaatcttcg gcagccgcaa cgaccgcttg ctgaaacaat accgtaaatc
     4021 cgttgccaga atcaacgcgc tcgaagaaca gatgcaagcc ctaagcgatg ctgatctgca
     4081 agccaaaact gccgaattca aacaacgcct cgccgacggt cagactttgg acggcatttt
     4141 gcccgaagcc ttcgccgtct gccgcgaagc gtcccgccgc accctcggta tgcgccactt
     4201 cgacgtgcag cttatcggcg gtatggtgct gcacgacggc aaaatcgccg aaatgcgtac
     4261 cggcgaaggc aaaaccctgg tcgccaccct cgccgtctat ctcaacgcgc tggccggcaa
     4321 aggcgtacac gtcgttaccg tcaacgacta cctcgcctca cgcgatgcgg gcattatgga
     4381 gccgctctac aatttcctcg gccttaccgt gggcgtgatt atttcagata tgcagccgtt
     4441 cgaccgtcaa aacgcctatg ccgccgatat cacctacggc accaataatg aattcggctt
     4501 cgactacctg cgcgacaata tggttaccga ccaatacgac aaagtgcagc gcgaattgaa
     4561 ttttgctgtt gtcgatgaag tggattccat cttgattgac gaagcgcgca ctccgctgat
     4621 tatctccggt caggcggatg acaacatcca gttgtaccaa atcatgaaca ccgttccgcc
     4681 ccacctcgtc cgtcaagaaa ccgaagaagg cgaaggcgac tattgggtcg acgaaaaggc
     4741 acatcaggtc atcctgagcg aagcaggtca cgaacacgcc gagcaaatcc tgacccaaat
     4801 gggattgctg gcagaaaacg actccctcta ttccgccgcc aatatcgccc tgatgcacca
     4861 ccttatggcg gcattgcgcg cgcattccct cttccacaaa gaccaacatt acgtcatcca
     4921 agacggcgaa atcgtcatcg tggacgaatt caccggccgg ctgatgtccg gccgccgctg
     4981 gtcggagggt ctgcatcaag ccgtcgaagc caaagaaggc gtggaaatca aacgcgaaaa
     5041 ccaaaccctt gcctccatca ccttccaaaa ctatttccgc ctgtacacca agctctccgg
     5101 catgaccggc acagccgata ccgaagcttt cgagttccaa agcatctaca acctcgaaac
     5161 cgtcatcatc ccgaccaacc gccctgtaca gcgcaaagac ttcaacgacc agattttccg
     5221 ttccgccgaa gaaaaattcg aagccgtcgt caaagacatc gaagaatgcc acaaacgcgg
     5281 gcagcctgtc ctcgtcggta cgaccagcat cgaaaactcc gaactggtct cccaccttct
     5341 gcaaaaagcc ggactgccac acaacgtcct caacgccaaa gaacacgaac gcgaagcgct
     5401 gattgtcgcc caggcaggca aagtcggcgc gattaccgtt gccaccaata tggcgggacg
     5461 cggtacggac atcgttttag gcggcaacct gaagcaccaa accgatgcca tccgtgccga
     5521 cgaaaccttg agcgacgaag agaaacaggc acaaatcgcc gcactcgaaa acggctggca
     5581 ggcagaacac gataaagtaa tggaagcagg cggcctgcac atcatcggta cggaacgcca
     5641 cgaaagccgc cgtatcgaca accaattgcg cggacgttct ggccgtcagg gcgaccccgg
     5701 atccagccgt ttctaccttt cgtttgaaga cccgctgctg cgcttattcg cactcgaccg
     5761 cgccgccgcc atcctcaacc gtctcgcccc cgaacgcggc gttgccatcg aacacaacct
     5821 gctgacgcgc caaatcgaag gggcgcaacg caaagtcgaa ggcagaaact tcgatatgcg
     5881 caaacaggtt ttggaatacg acgacgttgc caacgagcaa cgtaaagtca tttacagcca
     5941 gcgcaacgaa attctgacca gcaaagacat cagcgacctg atgcaggaaa tccgttctga
     6001 tgtcgtcagc gacctcgtgg atacctatat gcctcccgac agcatggaag aacaatggga
     6061 catcccgact ttggagaacc gtctggctgc cgaattcaga ctgcacgaag acatccaatc
     6121 ctggctgaag gcggacaatg cgattgacgg tcaagacatc aaagaacgcc tgatcgaacg
     6181 catcgaaaac gaatatgccg ccaaaaccga actggtcggt aagcaggcaa tggccgattt
     6241 cgagcgcaac gtgatgttgc aggtcatcga caaccaatgg cgcgaacacc tcgccgctat
     6301 ggactacctg cgacaaggca tacacctgcg cagctatgcc cagaaaaatc cgaagcagga
     6361 atacaaacgc gaagccttta ccatgttcca agacctgtgg aacggcatca aattccatat
     6421 tgcttccctg cttacttcgg ttcaaatcga acaaaaccct gtcgcggcgg ttgaagagca
     6481 acccatcggc aacatccagt ccatccattc cgaatcgccc gatatggaag aacttttggg
     6541 tcagtcgcaa accgatttgg ttaccgaggc ctttaatccc gatggaacgg atttcagccc
     6601 cgaagccttg gaagcgcggg gacaaatcgt ccaccgcaac gacccctgcc cctgcggcag
     6661 cggtttgaaa tacaaacaat gccacggcaa actggcttaa gcgtttgaac gcaaatgccg
     6721 tctgaacatc cgatttccgg tttcagacgg cattttgcct gaaccgccac atccgactgc
     6781 cattccgaaa aatcccgatt tcgtaccgtc cgtaccaaaa acagacatcc cgtccgcccc
     6841 acatcatgat tccatccgac ttcattgacg agcttttagc caaaaccgat attgtcgata
     6901 ttatcgacga gcaggttccg ctgaaaaaag gcggggcgaa ctatatggcg tgttgcccgt
     6961 tccacaagga aaaaacgccg tcgttttcgg tcagtccgac caaacagttt taccactgtt
     7021 tcagttgcgg ggcgcacggt tcggcgattg gttttgtgat ggaacatcag gggctgtcgt
     7081 ttccggaggc ggtgcagttc cttgccgacc gcgtgggtat gattgtgcct aaagtgcgcg
     7141 ggcaaaacga taatcccgaa gtccgtgccg agcgcaagaa aaaacagcag acgctggagg
     7201 aaacgacggc tgcggcagct gatttttatg cgcaacagct aaaattcaat ccggcggcaa
     7261 aagcttattt ggacaagcgc ggcttgagtg cagaagttat cgcgcattat ggtttgggct
     7321 acgcgcccga cggctggcag cctttggcgc aagtgttcca accgtatccg aataccgcgt
     7381 tggtggatac ggggatggtg attgacaatg aggggcggta ttacgaccgc ttccgccatc
     7441 ggattatgtt ccctatccgc aatccgcgcg ggcaggtcat cggtttcggc ggcagggtgc
     7501 tggacgactc caaacccaaa tatctaaact ctcccgatac acctttgttt gacaaaggga
     7561 aaaaccttta cggcttgtat gaggggcgtg ccgctgtcaa ggaagcggag cggattctgg
     7621 tggtcgaagg ctatatggac gtggtcgcgc tggcacagtt cggcgtgggc tacggcgtgg
     7681 cggccttggg cacggcaaca acagcggaac acgtcaaaat cctgatgcgt caggcggaca
     7741 gtatttattt ctgtttcgac ggcgacagcg cggggcgcaa agcggcttgg cgcgcgctgg
     7801 aaaacgcgtt gccgcagttg aaagacgaca aatcgctgca ttttttgttc ctgcccgagg
     7861 aacacgaccc cgacagttac atccgtgcct acggcaaagc gcaatttgaa gacgcgcttc
     7921 tgaatcaaag caagcctttg tcggagtatt tctgggaaca cctttcagac ggcattcatc
     7981 tcaatacgca ggaaggcaag gcggaattgg tgaaaaccag ttcgccgctt ttggcgcaga
     8041 ttaccgcgcc ggcattggct tatttgttaa aacaacggct tagcgaactg gtcggcatcg
     8101 accccgacaa cctcgcgcaa ctgctcggac aggaaacgcc gaagcggcac gtcaaacaaa
     8161 aaaactacaa actgcctccg atttccgtca aacagcccgt catgctgacg ctggtacagc
     8221 ggcaaatccg cagcctcttg ataaatccgg attgggctgc atatatagac ctgcccgatt
     8281 atctggcgtt ggacggtgat ttcgcctgcc ttgccaatct tgccgaatcg attaaaaacc
     8341 atgccgccgt acccgaaacc gctcaggttt tagagtatat gcgcggctcg ccttacgaag
     8401 aaacgataac ccgaatcttc cattcaacgc accaatcgga agaaatgaac agcagcagtg
     8461 aagaagattg cgagaatttc caaatcggca tgaaaaaact gctcaatgag ttaaaataca
     8521 gccaaatcga aacattaaaa caaaaaagcc tgcaatccgg cttaaatgaa agcgagaaaa
     8581 aacttttgct gtcgttgctg accgcaaaac aaaattgacc ggcggattcc gccacccgta
     8641 aacccgttat gccgtctgaa aagcacttac cccggctgca acaacgacac ctgcagaaca
     8701 cccatcccaa aaagccttca gacggcatca gagtacccta ctctgccacg ccttcaggtg
     8761 cgttcaaacg caaaccgtcg gcaccttacc aacagaaagc agacaatgtc cagaaaccaa
     8821 aatcacgaag aataccaaga cgatgcccgc cccctgacga ttgaggaaca acgcgctcgc
     8881 ctgcgccaac tcatcattat gggcaaagaa cgcggctaca tcacctactc cgaaatcaac
     8941 gacgcactgc ccgacgatat gtccgatgcc gatcaaatcg acaacatcgt cagtatgatt
     9001 tccggtttgg gtatccaagt taccgaacac gcccccgatg cggaagacat attgttaagc
     9061 gacaatgccg ccgttaccga cgatgatgcc gtcgaagaag ccgaggccgc cctttccagc
     9121 gcagattccg agttcggcag gacgaccgac cccgtccgta tgtatatgcg cgaaatggga
     9181 caggtcgacc tgctgacccg cgaagacgaa atcatcatcg ccaaaaaaat cgaaaacgcc
     9241 ctgaaaaata tggttcaggc catctccgcc tgcccgggat ccattgctga aatcttagaa
     9301 ctcatcgaaa aaatccgcaa agacgaaatc cgcgttgacg aagtcgtaga agccattatc
     9361 gacccgaatg aagtattgct caacgaattg ggcttggggc acttggaaac cacagcaccc
     9421 gagaaacctt ccaacgacaa ttcggacgaa aacgaagacg acgaagaatc ggaagaagat
     9481 gcggatgaaa tctcggcagc caatctcgcc gaattgaaac aaaaagtcat cggccacttt
     9541 gcccaaatcg aaaaagacta caaaaaaatg atcggccgtt tggaaaaaca ccacagccgg
     9601 cacaaagact atctcgccta ccgcgacgcg attgccaaca aactgctgga agtccgtttc
     9661 gccacccggc aaatcgacag cctcagcagc agcctgcgcg gaaaagtaga aaacatccgc
     9721 aaactcgaac gcgaaatccg cgacatctgc ctcgaccgcg tccatatgga gcgcgactac
     9781 ttcatccaaa acttcctgcc cgaaatcacc aatctggaat ggattgaaga agaaatcgcc
     9841 aaaggcaggg tttggagcga cgcgctcgac cgcttccgcc acgccatcct cgaaaaacaa
     9901 accgagttgg cggatatgga aaaagaaacc cgcatttcca tcgaagagtt gaaagaaatc
     9961 aacaaaaata tggtgtcgag cgaaaaagaa accgcagccg ccaaacagga aatgattcag
    10021 gcaaacttgc gcctcgtgat ttccatcgcc aaaaaatata ccaaccgggg cttacaattc
    10081 cttgatctga ttcaggaagg caacatcggt ttgatgaaag cggtcgataa gttcgaatac
    10141 cgcagaggct ataaattctc cacctacgca acctggtgga tccgccaggc aatcacccgc
    10201 tcgattgccg atcaagcgcg taccatccgc attccggtac atatgattga aaccatcaac
    10261 aagatgaacc gcatctcgcg ccaacacctt caagaaaccg gcgaagaacc cgattccgcc
    10321 aaacttgccg aactgatgca gatgcccgaa gacaaaatcc gcaaaatcat gaaaatcgcc
    10381 aaagagccga tttcgatgga aacccccatc ggcgacgacg acgattcgca cttgggcgac
    10441 ttcatcgaag atgccaacaa tgttgcgccg gccgatgcgg caatgtacac cagcctgcac
    10501 gaagtaacca aagaaatcct cgaaagcctg acaccgcgtg aggcaaaagt cctgcgtatg
    10561 cgtttcggca tcgatatgaa caccgaccac acgctggaag aagtcggcag acagtttgac
    10621 gtaacgcgcg aacgcatccg ccaaattgaa gcaaaggcac tccgcaaatt acgccatccg
    10681 acaagaagcg accggctgag aagtttcttg gacagcgaag acagcaaact ataaccctca
    10741 aaaaaaccgc aggtttcaaa tacctgcggt tttttcttac acaataaaca acgcttccac
    10801 atatcccaca ctcctatccc gagacctttg caaaactccc caaaatcccc taaattccca
    10861 ccaagacatt taggagattt ctcatgagca ccttctttca gcaaaccgcc caagccatga
    10921 ttgccaaaca catcgaccgt ttcccactat tgaagttgga ccaggtgatt gattggcaac
    10981 cgatcgaaca atacctgaac cgtcaaaaaa cccgttacct ccgagaccac cgcggtcgtc
    11041 ccgcctaccc gctgttgtcc atgttcaaag ccgttctgct cggacaatgg cacagcctct
    11101 ccgatcccga actcgaacac agcctcatta cccgcatcga tttcaacctg ttttgccgtt
    11161 tcgacgaact gagcatcccc gattacagca ccttatgccg ctaccgcaac tggctggcgc
    11221 aagacgacac cctgtccgaa ttgctcaaac tgatcaaccg acaactgacc gaaaaaggcc
    11281 taaaagtaga gaaagcatcc gccgccgt
//
LOCUS       DAXHSJ010000052        11081 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000052 DAXHSJ010000000
VERSION     DAXHSJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.052"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(68..1624)
                     /gene="dnaA"
                     /locus_tag="ACFM8U_001002"
     CDS             complement(68..1624)
                     /gene="dnaA"
                     /locus_tag="ACFM8U_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC6334259.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAVSKTAADILAERMKNLPHEPRQAAGSASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            1917..2051
                     /gene="rpmH"
                     /locus_tag="ACFM8U_001003"
     CDS             1917..2051
                     /gene="rpmH"
                     /locus_tag="ACFM8U_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC6334260.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            2054..2419
                     /gene="rnpA"
                     /locus_tag="ACFM8U_001004"
     CDS             2054..2419
                     /gene="rnpA"
                     /locus_tag="ACFM8U_001004"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC6334261.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            2485..2712
                     /gene="yidD"
                     /locus_tag="ACFM8U_001005"
     CDS             2485..2712
                     /gene="yidD"
                     /locus_tag="ACFM8U_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC6334262.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHGHDPVP"
     gene            2885..4522
                     /gene="yidC"
                     /locus_tag="ACFM8U_001006"
     CDS             2885..4522
                     /gene="yidC"
                     /locus_tag="ACFM8U_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC6334263.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQTAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDETKPFVLFGDGKEYT
                     YVAQSELLDAQGNNVLKGIGFTAPQKQYSLNGDKVEVRLSAPETNGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSAWILQPKGGQSVCAAGDCRIDI
                     KRRNDNLYSTSVSVPLAAIQNGAKSEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(4603..5316)
                     /locus_tag="ACFM8U_001007"
     CDS             complement(4603..5316)
                     /locus_tag="ACFM8U_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC6334264.1"
                     /translation="MMSPVLYLIPTPLGTPDTPCLLPHEQQAVVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQEIISQTVVQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(5375..5965)
                     /locus_tag="ACFM8U_001008"
     CDS             complement(5375..5965)
                     /locus_tag="ACFM8U_001008"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC6334265.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESSDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            6113..6616
                     /locus_tag="ACFM8U_001009"
     CDS             6113..6616
                     /locus_tag="ACFM8U_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC6334266.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSS"
     gene            6650..6829
                     /gene="rpmF"
                     /locus_tag="ACFM8U_001010"
     CDS             6650..6829
                     /gene="rpmF"
                     /locus_tag="ACFM8U_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC6334267.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            7004..7531
                     /locus_tag="ACFM8U_001011"
     CDS             7004..7531
                     /locus_tag="ACFM8U_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC6334268.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            7619..8674
                     /gene="plsX"
                     /locus_tag="ACFM8U_001012"
     CDS             7619..8674
                     /gene="plsX"
                     /locus_tag="ACFM8U_001012"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC6334269.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDEAPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            8810..9061
                     /locus_tag="ACFM8U_001013"
     CDS             8810..9061
                     /locus_tag="ACFM8U_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC6334270.1"
                     /translation="MKTLEKRMKALDKRIMKFEKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            9088..9504
                     /locus_tag="ACFM8U_001014"
     CDS             9088..9504
                     /locus_tag="ACFM8U_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334271.1"
                     /translation="MKTSTVVFGGFFMADNGERIQIPVLENPDIREINHFFSVSNFEK
                     KAGVLVFRIIPEPEFGHTELTVYFEKGYYLPMIQTILEGGDIEVKNLKTENYSGKARE
                     ILGDSYPIEHTSKNISTIQNIISEFMIQKQTPAPII"
     gene            9524..9727
                     /locus_tag="ACFM8U_001015"
     CDS             9524..9727
                     /locus_tag="ACFM8U_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334272.1"
                     /translation="MPSEAAWAFRRHFLRLKQHIGNPAPPVHGNLHLKTICMPNHTPI
                     PISVICTLPVLGNHRFFAYNSRI"
     gene            9754..10716
                     /locus_tag="ACFM8U_001016"
     CDS             9754..10716
                     /locus_tag="ACFM8U_001016"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC6334273.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDTPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            10763..>11081
                     /locus_tag="ACFM8U_001017"
     CDS             10763..>11081
                     /locus_tag="ACFM8U_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC6334274.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLIL"
BASE COUNT         2853 a         3320 c         2568 g         2340 t
ORIGIN      
        1 ttaatccgct atatctttac ccttcggacg gcatgggcaa tatcatgtcg tctgaaaacg
       61 ttttccatca gttttgaatc agaatcagca gcttttcata atcctgagcc aattccggat
      121 cttcttcgcg cagtttcgcc actgccctga tgccgtgcat aacggtcgta tggtcgcgtc
      181 cgccgaacga atcgccgata gacggcaggc tcaaagtggt cagttctttg gtcaggctca
      241 tcgccacctg gcgcggacgg gcaatgtttc gtgtccgttt cttaccgagc acatcgctga
      301 ttttgatgcg gtaatatttc gccaccgcat cgatgatgat atcggcggtg atgactttgt
      361 gcttctcggc aataatgtcc tgcaaagcgg tacgcgccaa atcgatgtcg atgacgggac
      421 ggttcataaa gcggctgctc gctccgacgc ggttgaacgc gccttcaagc tcacgcacgt
      481 tggaacggat cagattggca atgaacagcg cggcttcgtc ttcaatactg atgcccgccg
      541 cttccgcctt tttctgcaaa atggcgatac gcatttccaa ttcgggcggc tcgagttcca
      601 aagtcagtcc ccatgaaaaa cgggatttga ggcggtcgtc cataccttcg attttggcgg
      661 gtaaaacatc gcaagtgagg atgagctgtt ttttctcgtt gtggaaatgg ttgtacagat
      721 agaagaattc ctccatcgta cggtcttttc ctttaataaa ctgaatatcg tcgataatca
      781 gcaggtcgta ttgcttgtat tgctgcttga acacgtcgta ggtattgttg cgaaccgcct
      841 tcataaaact gcggatgtag tcgtccgaat gcatatagcg cactttggca tcgggacggt
      901 ttttcaacag ttcgttgccg accgcctgca caaggtgggt tttacccaaa cccgtactgc
      961 catagaggaa gaacgggttg taactctgcc ccgggctttc cgcaatcgcc tgcgccgccg
     1021 ccgccgcaag gcggttgccc ttgccttcga ccaatgtgtc gaacgtataa tccggcgaca
     1081 gattggtctg ctcgtaacgc gcctcttccg catcgcgctg tgcatccgtc cgtgctttgg
     1141 caactgccgc cgattccggc cgggaagcag acccggcagc ctgacgcggc tcgtgcggca
     1201 ggtttttcat acgttctgcc aaaatatccg ccgccgtttt cgacacagcg ggtttgacag
     1261 gctcttcaga cggcagctcg tccaacagaa cctcctgcac aggcatctcc tccgaccccg
     1321 cgtgcaagga cggctcggca ggttcgacag caccttcaac cgccgccatc tcataacgta
     1381 cgccttctcc cggtttgaat acgaaggcgg aacggccggc agccaactct tccctcaccg
     1441 cttctatttt tccggcaaac tggctcttga gcatattgca ggcaaactga ttcttgccgt
     1501 acaccaccca tacgccaccc tcctcaccga cggtaagggg cgcaatccat tgcgcaaact
     1561 gcccttgagg caacatatcg tgaagacggc ggaggcacag cggccaaaac tctgctaatg
     1621 tcatggataa gctcgaatcg gtaaaaatga aatcgaaaac aaagaaaata taatattttc
     1681 aaaaagaaaa caaatctgtt gaacacacat cggttcaaaa tgcagcgaac cgaattatac
     1741 cgactcgccg atattttatc cacaacccgt gcaaaattta tccacagaaa ggcggcagaa
     1801 atccgcaggc aatcgggcaa tcctcctgca aagtttctat attgattgac aaaagcggca
     1861 aattggagtg taattcacgg tttaattatc tacccattct atttttagga aacatcatga
     1921 aacgcactta tcaaccttcc gttaccaaac gcaaacgcac ccacggcttc ctggtgcgct
     1981 ccaaaacacg cggcggccgc gcagtattgg ccgcacgccg tgccaaaggc cgcaaacgcc
     2041 tggcggtata attttggact accgcttcgg aaggcagtac cgcttgttaa aaacggatga
     2101 tttttcatcc gtttttgcat tcagaaaccg ccgcagccgc gacctgctgc aagtttcgcg
     2161 ttcaaacggc aacgggctgg accatccccg catcggtctg gtggtcggca aaaaaaccgc
     2221 caaacgcgcc aacgaacgaa attatatgaa gcgcgtcatc cgcgactggt tcagattgaa
     2281 caaaaaccgg ctgccgccgc aggatttcgt cgtgcgcgtc cgccgtaaat tcgacagggc
     2341 taccgcaaaa caggcaaggg cggaactggc acaactcatg ttcggcaacc ccgcaaccgg
     2401 atgcaggaaa caggcatgat cagaacggta ctctgcaggc aaggttcaga cggcaacggg
     2461 tttcccatac taaggaacat cccgatgaac ttcctattgt ccaaactcct gctgggactg
     2521 atacggttct accaatattg catcagcccg ctgattccgc cgcgctgccg ttatacgccg
     2581 acctgttcgc aatacgcggt cgaagcggtc aaaaaatacg gcgcattcaa aggcggtcgg
     2641 ctcgccatca agcgcattgc acgctgccac cctttcggcg gacacggaca cggacacgac
     2701 cccgttccct gacccgacgc aatattcaaa ttgcacgctt tccttttatt tcccatcggt
     2761 ttctatataa tgccgtctga accttcgggc tggcggcacg accgtcgggt atgaagcccg
     2821 cccttattcc ccgtctatcg gaacacgcaa cctgcggcat ttccgaccat tcaggaaact
     2881 ctatatggat tttaaaagac tcacggcgtt tttcgccatc gcgctggtga ttatgatcgg
     2941 ctgggaaaaa atgttcccca ccccgaaacc cgtccccgca ccccaacaga cggcacaaaa
     3001 acaggcagca accgcttccg ccgaagccgc gctcgcgccc gcaacgccga ttaccgtaac
     3061 gaccgacacg gttcaagccg tcattgatga aaaaagcggc gacctgcgcc ggctgaccct
     3121 gctcaaatac aaagcaaccg gcgacgaaac caaaccgttc gtcctgtttg gcgacggcaa
     3181 agaatacacc tacgtcgccc aatccgaact tttggacgcg caaggcaaca acgttctgaa
     3241 aggcatcggc tttaccgcac cgcaaaaaca atacagcctc aacggcgaca aagtcgaagt
     3301 ccgcctgagc gcgcccgaaa ccaacggact gaaaatcgac aaagtctata ccttcaccaa
     3361 aggcagctat ctggtcaacg tccgcttcga catcgccaac ggcagcggtc aaaccgccaa
     3421 cctgagcgcg gactaccgca tcgtccgcga ccacagcgaa cccgagggtc aaggctactt
     3481 tacccactct tacgtcggcc ctgttgttta tacccctgaa ggcaacttcc aaaaagtcag
     3541 cttctccgac ttggacgacg atgccaaatc cggcaaatcc gaggccgaat acatccgcaa
     3601 aaccccgacc ggctggctcg gcatgattga acaccacttt atgtccgctt ggattttgca
     3661 gcctaaaggc ggacaaagcg tttgcgccgc tggcgactgc cgtattgaca tcaaacgccg
     3721 caacgacaac ctgtacagca ccagcgtcag cgtgccttta gccgctatcc aaaacggcgc
     3781 gaaatccgaa gcctccatca acctctacgc cggcccgcag accacatccg tcatcgcaaa
     3841 catcgccgac aacctgcaac tggcaaaaga ctacggcaaa gtacactggt tcgcttcccc
     3901 actcttctgg ctcctgaacc aactgcacaa catcatcggc aactggggct gggcaatcgt
     3961 cgttttgacc atcatcgtca aagccgtact gtatccattg accaacgcct cttaccgctc
     4021 tatggcaaaa atgcgtgccg ccgcacccaa actgcaagcc atcaaagaga aatacggcga
     4081 cgaccgtatg gcgcaacaac aggcgatgat gcagctttac acagacgaga aaatcaaccc
     4141 gctgggcggc tgcctgccta tgctgttgca aatccccgtc ttcatcggat tgtattgggc
     4201 attgttcgcc tccgtagaat tgcgccaggc accttggctg ggttggatta ccgacctcag
     4261 ccgcgccgac ccctactaca tcctgcccat cattatggcg gcaacgatgt tcgcccaaac
     4321 ttatctgaac ccgccgccga ccgacccgat gcaggcgaaa atgatgaaaa tcatgccgtt
     4381 ggttttctcc gtcatgttct tcttcttccc tgccggtctg gtattgtact gggtagtcaa
     4441 caacctcctg accatcgccc agcaatggca catcaaccgc agcatcgaaa aacaacgcgc
     4501 ccaaggcgaa gtcgtttcct aaatgccgca gcatgaaaaa tgccgtctga aacctgttca
     4561 gacggcattt ttattgctca ccccctatcg gggcggaaat cttcaacctg catacatcac
     4621 aaaaatcgtc gggcgttttt tcagattggg catttctttt ctttttcgcc actgcacaac
     4681 cgtctggctg atgatttcct gagtcggcaa cgtcaaatcc gtagccgtgc acaagcgcgt
     4741 ttcaggatgc aggttttcca ccgcatccgc caacaacaca tcgttgcgat acggcgtttc
     4801 gataaacagc tgcgtttcat tctgctggcg cgaacgttgc tccaaagcct tcaaactctg
     4861 aatccgctcg tttttttcag acggcagata acccttaaac gcaaaactct gcccgttcgc
     4921 acccgaagcc atcaaagcca gcagcaggct ggaaggccca accagcggac gcacttcaaa
     4981 accgtgttta tgcgccaaag ccaccagatt cgcacccgga tcggcaacag ccgggcaacc
     5041 cgcctcgctg acaatgccca tactgcgccc ttcttgcaaa ggtttcagca attccggcaa
     5101 agtcttcaaa tccgtgtgtt cattcagcgt ttgcaaattc agttcgcgga taggcattgt
     5161 cacgcccaaa tgtttcaaat gcgcgcgcgc cgttttttcc gcttccacga caaaatccgt
     5221 cagtccgaca accgcctgct gttcgtgcgg caacaggcac ggcgtgtcgg gcgtgcccaa
     5281 aggcgtagga atcaaataca aaacaggaga catcattccc tcactcgtca gttaaaaatg
     5341 ccgtccgaac ccttcagacg gcataaacgg gcagttacag aacctccacg ccctcatttt
     5401 tcaagaaatc gaccagacgg aaaaccggca aaccgattaa agcattcgga tcgctgcttt
     5461 caatcctttc gatcaacaat gcacccaaag cctcgctctt caacgcacac gagcaataaa
     5521 ccgcatcagg ctcgcgctcc aaatagcgga ggatgtgtag ctcgtccaac ttcctcatca
     5581 cgaccaccgt cttatcaata tgccgctgca tcctgcccgt aaccgtattt agcagaacga
     5641 ccgcgctgta aaactcaatc tccctgccgc tcaaatgcat caacatcttt tgcgcattag
     5701 caaggttcat cggcttgccc cactgcctgc cgtcgcacca cgccacctgg tccgcaccga
     5761 caatcaacgc ctcggggaaa cgtcctgcca acgaccgcgc cttaccctcg gcaaggcgca
     5821 atgccgtctg aggggcagac tcccccaaca tcggcgtttc gtcaaaatcg ggggacgccg
     5881 cctgaaaggc aatgccgagc ctttccatct gttcgcggcg gaaaaccgaa ctcgtaccca
     5941 aaatcaaagg cagttccaaa cccatcccat cctccttacc gttgaaaaca cgcccgaagg
     6001 ggcggtaaaa tccagccatg cgccgaaaca cggatacccg ccttcggcgt accgcaacat
     6061 ttttcttaaa aatattgacg ttagaacatc taaattatat catatctcgt ttatgtcaga
     6121 ccctaatttg attgacccgg aaattttcgc cgccgaaggg cagaatctgc aaggcagttt
     6181 tctgctggaa gaattggatg aacgcgtcag ttcgcacgat tatcccgccg acaggcagac
     6241 caaaatatcg tttacactga ccggcggtcg cgatcggctg caacgcctgt tcctcgacct
     6301 gaacgtcaaa gccgatatgc ccctgatttg ccagagatgt atcaaaccca tgccgttcat
     6361 gcttgatgaa agcagccgta tcgtcctgtt ttccaacgaa gagtccttgg acgaatccat
     6421 gcttgccgac gaagaactcg aaggcatact gattgaaaaa gaactcgacg tgcgcacatt
     6481 ggtagaagac caaatcctga tgtccctgcc cttttcgccg cgacacgaag actgcggcga
     6541 caatgggaca ctggaagaag tcaatcggga caaacccaac ccctttgctg ttttggcagg
     6601 tttgaaaagc agttaattag gacacagttt atttatctag gagcttgaaa tggccgttca
     6661 acaaaacaaa aaatcccctt ccaaacgcgg tatgcaccgt tcgcacgacg cgctgaccgc
     6721 gcctgcactg tctgtcgaca gcacaaccgg cgaagtacac cgcccgcacc acatctcccc
     6781 caacggtatg taccgcggcc gcaaagtggt caaagccaaa ggcgaataat ccctattcga
     6841 ctgactgaaa aagccagaac attgccatgc aattactggc tttttttgca ttggacgcac
     6901 catccgtcca aactttcgcc atacgtcaac acacaggggc aaagcgttcc gtataatacc
     6961 ccgtgaaaat attccaaaag ccccaaccac caaggaaatt ccgatgaaac agaaaatctg
     7021 gtacacctac gatgacatcc accgcgtcat caaagcattg gcagaaaaaa tccggaacgc
     7081 cgacatcaaa tacgatgcca tgattgccat cggcggcggc ggctttattc cggcacgtat
     7141 gctgcgctgt tttctggaaa ttccgattta tgccgtaacc accgcctatt acgacagcga
     7201 caacgaagga caggttaccg aagaagtcaa aaaagtccaa tggctagacc ccgttccaga
     7261 agccctgcgg ggcaaaaacg tactcgtcgt cgatgaagtg gacgacagcc gcgtaaccat
     7321 ggaattttgc ctgacagagc tgctcaaaga agacttcggt acgatcggcg tcgccgtact
     7381 gcacgaaaaa atcaaagcca aagcaggcaa aatccccgaa ggcattccct atttcagcgg
     7441 catcaccgta gaagactggt ggatcaacta tccgtgggac gcactcgaca tcgacgaaca
     7501 caaccgcctt gccgaggccg gccgaggctg accctttcag acggcatatt ttccgaaccg
     7561 atgccgtctg aagcccgcac gacccctgcc gcagaccgaa aacctaccgg agaaccctat
     7621 gattacactt gccgtagatg ccatgggcgg cgaccaagga cttgccgtta ccgtacccgg
     7681 cgcaaccgca ttcctccaag cacaccccga tgtccgcctg attatgaccg gcgacgaaac
     7741 gcaactgcgc caagccctga ccgcggcagg cgcaccgatg gaacgcatcg acatctgcca
     7801 taccacccaa gttgtcggca tggacgaagc cccgcaatcc gccctgaaaa acaaaaaaga
     7861 ctcctccatg cgcgttgcca tcaaccaagt taaagaaggc aaagcccaag ccgccgtatc
     7921 cgcaggcaac acgggcgcgc tcatggcaac cgcacgtttc gtcctcaaaa ccattcccgg
     7981 catcgaacgc cccgccatcg ccaaattcct tccttccgac accgaccacg ttaccctcgc
     8041 cctcgacctc ggcgcaaacg tcgactgcac gcccgaacag ctcgcccaat ttgccgttat
     8101 cggcagcgaa ctcgtccacg cactccatcc tcaaaaagga cagccgcgtg tcgggctggt
     8161 caacgtcggc acggaagaca tcaaaggtac ggacaccgtc aaacaaacct acaaactgct
     8221 gcaaaacagc aaactcaact ttatcggcaa catcgaaagc aacggcatcc tgtacggcga
     8281 agcagatgtc gtcgtcgccg acggctttgt cggcaacgtc atgctcaaaa ccatcgaagg
     8341 cgcggtcaaa ttcatgagcg gagccatccg ccgcgaattc caaagcaacc tgttcaacaa
     8401 acttgccgcc gttgccgccc tacccgccct caaagggctg aaaaacaaac tcgatccgcg
     8461 caaattcaac ggggccatcc tgctcgggct gcgcggcatc gtcatcaaaa gccacggcgg
     8521 cacagacgaa accggtttcc gctacgcact cgaagaagcc taccacgaag ccaagtccgc
     8581 cggcctttcc aaaatcgaac aaggcgttgc cgaacaactc gccgcactcg aaactgccaa
     8641 agccgtccaa aacgaaaatg ccgacggtct gtaacacaca cgatgccgtc tgaacgcccc
     8701 cgcccctttc agacggcatc cgcccgcacc aaacctgcgg gcgcggacgg cgatgcgcct
     8761 gtccggcact tcccaaatac ttccctgtaa aataaggagt atttgaaaaa tgaagacatt
     8821 agaaaaacgg atgaaagctc tagacaaacg gattatgaag ttcgaaaaat cccttgaagg
     8881 caggcttgat gcccgtctga ttgaatccgc attggattat attcattatt cggaacgttt
     8941 tttggctttt gaaatcctgt gtacttatat cgaagatttc gatgtccggc tgacggaaca
     9001 agaatcccgg gaaatttctt ttatcaacaa ggaatttgag atagaaagca cgtccgatta
     9061 accaataaag ccaatgggtt gataaacatg aaaacatcga cggtcgtttt tggcggattt
     9121 tttatggcag acaacggaga gcgaatccaa atccccgttt tggaaaatcc tgacattagg
     9181 gaaatcaatc actttttttc cgtatcaaat tttgagaaaa aagccggcgt ccttgttttc
     9241 agaatcatcc ccgagccgga atttggccat accgaattaa ctgtctattt tgaaaaagga
     9301 tattatttgc caatgattca aacaatttta gaaggcgggg atattgaagt caaaaacctt
     9361 aaaacggaaa attacagtgg gaaggcaagg gaaattttgg gagattccta ccctattgag
     9421 catacctcta aaaatatttc tacaattcaa aatataattt ctgaatttat gatacaaaaa
     9481 caaacaccgg caccaataat ttgatggaaa cccaacaatc aaaatgccgt ctgaagcagc
     9541 ttgggctttc agacggcatt tcctccgctt aaaacagcat atcggcaacc ccgccccgcc
     9601 cgtccacggc aatctgcatc tgaaaaccat ctgtatgcca aaccacaccc ccatcccgat
     9661 ttccgtcatt tgcaccctgc ccgtattggg caatcatcgg tttttcgctt acaatagccg
     9721 aatctgaacc aactatccaa aaaggccgtt cccatgcagt atgccaaaat ttccggcacg
     9781 ggcagctatc ttcctgccaa ccgcgtcagc aatgacgacc ttgcccaaaa ggtagatacc
     9841 tcggacgagt ggattaccgc gcgcacgggc atcaagttcc gccatattgc agccgaaaat
     9901 gagaaaacca gcgatcttgc cgccgaagca gcccgccgcg cgctggatgc agccggatta
     9961 gacagcggcg aaatcgattt gattatcgtg gcgacggcaa cgccggatat gcagtttcct
    10021 tctaccgcca ccatcgtgca gcaaaaattg ggcatcacca acggctgtcc cgcgtttgac
    10081 gtacaggcgg tgtgcgccgg ctttatgtac gcgctgacca cggcaaacgc ctacattaaa
    10141 agtggcatgg cgaaaaacgc gctggtcatc ggcgcggaaa ccttcagccg catcgtcgat
    10201 tggaacgacc gcacaacctg cgtattgttc ggcgacggcg cgggcgcggt ggttttaagc
    10261 gcgtcggaca cgccgggcat catccacagc aaactcaagg ccgacggcaa ttatctgaaa
    10321 ctcttaaacg tccccgggca aatcgcctgc ggcaaagttt ccggttcgcc gtacatttcg
    10381 atggacggtc ccggcgtgtt caagtttgcc gtcaaaatgc tgtccaaaat cgccgatgac
    10441 gttatcgaag aagccggtta caccgccgct caaatcgact ggattgttcc ccatcaggca
    10501 aaccgccgca ttatcgaatc gaccgcgaaa catttaggtt tgagtatgga caaagtcgtc
    10561 ctgaccgtcc aagaccacgg caacacatcc gccgcatcga ttccgctggc tttggatacg
    10621 ggcatccgca gcggacaaat caaacgcggt caaaacctgc tgctcgaagg catcggcggc
    10681 ggtttcgcgt ggggcgcggt gctgttgcaa tattgaaccc gatgccgtct gaaacaggct
    10741 ttcagacggc atttcccata tcatgaagcg gcaggctttc ttcaaactga tggcgtgtgc
    10801 ggcatttctg tctgccgttt cgctgcgcct ccccgtattg ggcgcgtgtt acgcaatatt
    10861 gtccctctat gcgtttgcac tttacggcat cgacaaacgg cgtgccgtgc ggggaaaacg
    10921 ccgcattccc gaacaccgcc tgctgctgcc tgccttgttc ggcggttggg cgggcgcata
    10981 cttgggcagc aggatattca ggcataaaac ggcgaaaaag cgttttgttg tgctgttccg
    11041 tctgactgtt tcaggtaatg tcctggcgac cctcatcctg a
//
LOCUS       DAXHSJ010000053        10767 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000053 DAXHSJ010000000
VERSION     DAXHSJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.053"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(120..815)
                     /locus_tag="ACFM8U_001018"
     CDS             complement(120..815)
                     /locus_tag="ACFM8U_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFC6334275.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(986..1645)
                     /locus_tag="ACFM8U_001019"
     CDS             complement(986..1645)
                     /locus_tag="ACFM8U_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFC6334276.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(1848..3958)
                     /locus_tag="ACFM8U_001020"
                     /pseudo
     CDS             complement(1848..3958)
                     /locus_tag="ACFM8U_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(4144..4749)
                     /locus_tag="ACFM8U_001021"
     CDS             complement(4144..4749)
                     /locus_tag="ACFM8U_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6334277.1"
                     /translation="MTRQSLQQAAESRRSVYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(4798..5196)
                     /locus_tag="ACFM8U_001022"
     CDS             complement(4798..5196)
                     /locus_tag="ACFM8U_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6334278.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLASILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            5304..6215
                     /locus_tag="ACFM8U_001023"
     CDS             5304..6215
                     /locus_tag="ACFM8U_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6334279.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAAGALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            6205..7506
                     /locus_tag="ACFM8U_001024"
     CDS             6205..7506
                     /locus_tag="ACFM8U_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC6334280.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(7574..7744)
                     /locus_tag="ACFM8U_001025"
     CDS             complement(7574..7744)
                     /locus_tag="ACFM8U_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334281.1"
                     /translation="MLLYRLLGGAEIECGRCPVPPMTDWQHFLPAMGTVSAWVAVIWA
                     YLMIESEKNGRY"
     gene            7901..10051
                     /gene="dinG"
                     /locus_tag="ACFM8U_001026"
     CDS             7901..10051
                     /gene="dinG"
                     /locus_tag="ACFM8U_001026"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC6334282.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            10088..10702
                     /locus_tag="ACFM8U_001027"
     CDS             10088..10702
                     /locus_tag="ACFM8U_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC6334283.1"
                     /translation="MNFTRLLNQVLSTVQKKGGTFSDSPLNSFGGGALVAGIASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAKRRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT         2550 a         3058 c         2732 g         2427 t
ORIGIN      
        1 ctatatattc tgatttaaaa ccataaggct ttaagcaatc atctcttcta taaagcctaa
       61 atacaaaagg ccgtctgaaa tcctattttc agatggcctt tactcattca atcctcaact
      121 tattgcggct ttttctgctc ttcgcgtact ttatccacca actggtcaat cgtggtcata
      181 ccggactgcc agtctttaaa ttcaacttgg tatttgccgc cgacaatcac agtcggtgtg
      241 ccgctgattt ggaatttatt ggtcaactct tccatttgag ccgcacgcgc ttggctttca
      301 ggagcctcaa atgcagccaa tacttttttg ccgtcaaacg ctgtttgctc ggacagccat
      361 tttttcaggg tatcggtatc ggccagattg attttttgat taaccatcgc atcgaaaata
      421 tggctgttgg ctttatctga ttcaccggcc atttccactg cggccgccaa acgtgccaaa
      481 ggtttcattt catcacccca cacgacatgc tcccggcgca tataggtatc gtctttaaac
      541 gttttgatgt gctcgctcaa gaccggctca agatgggcgc aatgcgggca gaagtagccg
      601 aaaaattcca atacttcgat tttaccggcc tgctgttgcg gaataggcgt agacaataca
      661 gtgtagttca caccttcgtt caactcagca ggggctgccg gagcagatga gctgctttgg
      721 gcgctgtctg ccggaacact ggtttcagcc tgtttgctac aagcggccaa tgccaacagg
      781 gtcaatgaag tcaaagctaa ggttttcagt ttcataggta tctcttgtgt gtcagattaa
      841 tgtgcggatt ttatggcatt ttattgaagg cgtgttcgat ttgagtgaaa aaatgtgttt
      901 agttttattt cagcctttat aaaggccgtc tgaaatatgg cttagataat tagccttatt
      961 ttcagacggc ctttgttcta gtaatttacg gtttcggaca ttttgtcttc aatccgtcga
     1021 tgtgttccat taagtcaggt gggacatgat tccacatagg atcaagaata aaatcgatac
     1081 cttcccgtct ggctaatttg gctgctggga caaaatcgga atcaccagaa aacaagatga
     1141 ttttatcggc ttgttttttt aaggtaatag atgaaatatc caagcctata cgcatatcta
     1201 cacctttttg tttaacatcg agcacgacat cgttttccgt aatttgagtt gacgggtttt
     1261 tcagaagatt tttcatgact tggggtttta atatccatgc tgtattgtcc gacagcttgc
     1321 ctaaacgtaa cgctaattta cgcttcttct tcaattcggt atgcagattg caacgaaata
     1381 tggcttccgg agtctttgaa aaatctatgg cacgatgaga tacgggattg tgcattttct
     1441 tttcaaacgg cacgcagtcg taaaagaaaa ttctatataa atcatccatt tgatctgtgc
     1501 cgttgatttt atctttcaga tgcagcatcg aatatcggaa agccaaatca gcaattcttt
     1561 ttgcatcgtg tggattttga gacaggccta atgaccgtac ccgcttaata aaaaatgcac
     1621 cgtcaatcaa aatggcggtt ttcatattgc ttcccctata tttgtcaaag atataaaaaa
     1681 gcccttggga tcggcatacc cgctatgatt taatgggtaa tgcgtactgc caagggcgct
     1741 atttcttttt ggattatact gatgtagata aagcctgtca aaaagttcgt gtgctgaatt
     1801 tacggattat caggattttc agacggcatt ctgttgcacg aacgggatta gaaactgtaa
     1861 ttaacccgcg ccgtataagt gcgcggcgcg ccgggcatgg catccgaacg ccaatatttt
     1921 tgattgagca gattggctgc ggcaaaggta atgttaacat ttttatggtt ccagccaagc
     1981 atggcatcaa ctcgggcaaa gcctggaagc gtagtcactt ctttatttct tgagttgtaa
     2041 ccgtagcgtt tgcctgtacc ggttacgccg atttcgccgt agaggttttc ggtcggggta
     2101 taacggaaaa acaggttgcc ggtaacgttg ctggtgttat tcaaatggat gcccactcgg
     2161 tcgggatttt ctttgtcttc aacgactttc gcctgaatca cgcccaacga accgcgcaga
     2221 tagagttttt tggggatgat ttgcccgatg gcggacaatt ccacgccacg cgaacggtgt
     2281 ttgccgccga ctgcccaagt gtagggatta ttttgtgcgt cggggcggta gcggatattg
     2341 aagcgttcga tttggtaggc ggacaatgtg gtgctgaggc ggtcgtccag ccaactgctt
     2401 ttaacgccgg tttcgtattg gcgggtgtac tcggggtcag cgttgaacac ggcagaagat
     2461 gacgtatcga tgctcaaata gccgccgcgt ccgccataag gcgcgaaacc tttgttatac
     2521 gaggcgtaaa gtgtgtgaac ggggttgatg ttccacactg cgccgatgtt ggggctgaac
     2581 gagtggccgc tgtactggcg gctgctgccg gtgagtttgt tttcggaatt aaaggtgtat
     2641 ttgtcgtaac ggccgccgag gacgaatttc aaatcgggcg tggcggagaa gatgttttgc
     2701 acaaagatgc cgtaggagtc ggctttgtgg cggttttggg tgaggatagg ctgcaatctg
     2761 cccgaagccg gccagcttgc tcggtcgtat ggatcgatgg aagcagtaaa ggcgcggctg
     2821 taacctaagg tcgggttgcg gtgttcgcgg ctgtaatcca tgcctacggt caagtggttt
     2881 tcaaaacggc cgatggtgta gtcgccgttt agcgtgaaat tggacgacag ggttttgttg
     2941 tcggtctgct gccaggcgta gttacgtttg attaagttgc cattttcgct gcctgcatag
     3001 aaatgatcaa aatcctgcgc cgccgtgcgg tgggcgagct gccattgggc acgccatttg
     3061 tcgttgaagg cgtatcaagg tcggagcgcc aaacttgcag cttgtctttg acaaaatcgt
     3121 tccggtgggc gaaccccatg cggtaaggca gtccgaagcg gtcgtacacg gacttggtcg
     3181 gactgcggtc gggcgtgcgc tccacattgt cgtaggtgta ttgccccgtc cacttcaagc
     3241 cgttgtcgag tttgacggta atgctgggcg aaaccatgac atttttgctg tctatgccgc
     3301 tgcggaacga attggcgcgc ccgacttcgc cggtgagacg gatggcgacg tttttgttca
     3361 acacttcgtt aatgtccata ttcaggctgc ggtttgccca cgaaccgtaa accgcaccga
     3421 tgttgcggct ttgtttgaag ttggcgtatt tgctgaccat gttgatgacg ccgccgccgt
     3481 tggtgcggcc gtaaagcacg gaagacgggc ctttcaggat ttccacgcgc tcgatgttgg
     3541 cagtactgcg gcgcacttgt ccgctttcgc gcacgccgtc gcggtaaata tcggatgcgt
     3601 cggcttgaaa accgcgcagg aaaatgcttt cgccgcgcat atcgtaggca gcgtcgatgc
     3661 cggcattgcc ttcgaggatg gaactcaaat cgttcgtacc gtaatttttg tttttctgga
     3721 tattgagcgt atcgatggtt tgcggcgttt ctttgatgag ctgtccgttg cgggtaacgg
     3781 cggcttcgtc gtagttgatg tagcctttga gtacgctggt gtcggactgt ccgaccacgg
     3841 aaacggtggg cagagtggcg gtgtaatgtt caccattgtc ctgcgtatcg gcggcagcaa
     3901 cagggaagga agcaataatc agcgtgggta ataaagctaa atgaaatgat attttcattt
     3961 ttatactcaa tttaacaaaa caaccgaatt atattgcctc acggaggaaa tgagaataat
     4021 ttcttttaac tatattgaac atgatatttg taaacaaagg tctcagaatg cggaaaactc
     4081 gccgcctgat actgaaaaat gccgtctgaa cagggttcag acggcatttt tttgaccgcg
     4141 aaattatgcg ccgaacactt tcaaacgttc tgcaacgggt tcaaaggtct tttcacctgc
     4201 cgccccttca ataccgccga taaccatttg tgcgcgcaac aaccagtttt cggggatatt
     4261 ccacgctttg gcaatcgccg catcgggcaa gggattgtaa tgttgcaggt ttgcacctac
     4321 gccgaccgcg gcaagtgtcg tccaaacggc atactgcacc atagcgttcg cctggtccgc
     4381 ccaaacggga aagttggcgg cataagcagg gaactgctcc tgcaaacctt tgacaacatt
     4441 ttgatcttca taaaacaaaa tagttgccgc acccgcctta aacaggttca atttttgcgc
     4501 ggtcggttca aaactgtcgg caggcacgac ggcacgcagc gcgtcttcga caaattgcca
     4561 caccttatca tgctcttcgc caaacagcac gaccacgcgg gcagattggg aattgaacga
     4621 agaaggtgtg tgcaaaacgg cgtgttcgac gatttggaca acttcatctt tgccgacggg
     4681 caggttttta tttaacgaat aaacggaacg gcggctttcg gcagcctgtt gcagagattg
     4741 acgggtcatt tcttttcctc aaaaacattc aacaaaatac actttaggca gtccggttca
     4801 ggctttacgc ttgaaaaaca gcctgtccag cgaccatgct ccgccgcccg ctgccgcgat
     4861 atagaggaat acgaagcaga acagcactgc ggactcgccg ccgttggcaa tcgggaacaa
     4921 agcatttccg gaagcgtgcg ccataaaata ggcaaccgcc atctgaccgg acaaaacaaa
     4981 cgcggcaggg cgcgcaaaca ggcccaacac cagcaaaatg ccgccgacaa tttctaaaat
     5041 actggcaagc agcaacagcc cgccgggcga accgctgccc atttcaatgg ggaaggcgaa
     5101 gattttcgac gtaccgtgca acaaaaacag gtaggcggtt acgatacgca aaacagaaag
     5161 caaaaccggt tggatacggt tgcagcaatc ggacataaaa gctcctttga tatttgtctt
     5221 catttcaggc ggtagagtat agatgtttcc ccttttcaaa tatatactgc ttttattgat
     5281 acaacttttc cacacaagaa aatatggaca ccctgttcag cctcaaggtt ttccgccaag
     5341 tcgtccaaag cggcggcttc acccgcgccg ccggcgcgct cggcatctcc accgcaatgg
     5401 caagcaaaca cgtcagccac ttggaaaaca ccgtccaagc caaactcctg caccgcaaca
     5461 gccgcaacct cagcctgacc gaagccgggg aagaatacta ccggcaatgc agttacgcgc
     5521 tcgacacgct cgacgatgcc gcgcaaaaag ccgccggggg gacggaaaaa ccgcaggggc
     5581 tgctgcgcgt aacgatgcca ctgtggtttg ccggcagcca gatatgcaac tggctggcgg
     5641 aataccgcga acgttatccc gaagtgacat tagaactgat tttggacaac cgccacgtcg
     5701 atttgattgc cgaaggcgtg gatttggcgt tgcgcgtttc ccaaaccctg tccccgtcac
     5761 tgattgcgcg cccactggcg gaaatcgaat ttgccctgct tgcctcgccc gacttcctga
     5821 ggcgcaacgg cgtgccggaa acgccggaag aagtggcggg gctgcccgcc gtcctgccga
     5881 cctacaccaa ccagcagaaa ctcgacctca cccgcaaatc ggacggcaaa aaataccggc
     5941 ttgaactgac ccccgtcatc cgtaccgaca acacgctgat gatgcgcgaa atgattaagg
     6001 cgggcgcgtg catcggttat cagccgcttt gggcggcgga acacgatttg cgctgcggca
     6061 cgctggtgag gctgctgccc ggatacgccg tcccgaccga ccggctgaat gccgtttatg
     6121 cagacagggc attcttaagc gcgaaagtcc gcagcttcat cgattttctg aacgaaaaaa
     6181 tcgccagcag gaaaggctgc cgaaatgccg tctgaaaccg cccgccccct ttatgcggac
     6241 acgtgcgccg cacacacgct cgtctggttc cgccaaaacc tccgcatccg cgacaacgcc
     6301 gccttatgca ccgccgttgc cgaaggtttg cccgttatcg gtatttggat tgacgatgcc
     6361 gaaacagaca accctcgccg cgccgcgttc taccgccaat ccgccgccga actcgcccaa
     6421 gggcttgcag ggcgcggcat cccgctctac acggcggcat cgcctgccga gctcgtccgg
     6481 ctcgccgtcc gcctcaatat ccgcgccgtc atcgccgacg aatcccatac ttttgccgac
     6541 aaactcgccg acaacgccct ttggcatgaa ttggacaaac acggcatcgc gttaaccttc
     6601 gtcaatgacc gcgccgtttt cggcaaaacc gacctgatac ccgacggcgg cacggcacac
     6661 accgatttcg accgctaccg cgaagtatgg ctcgaccgct tttccaagca gccccccgcc
     6721 ggtccggacc tattcgcggc ataccgccaa cccttccccg aaaacctttc cgccccgccg
     6781 cctgccgccc tttcagacgg catcttcctg ccgcaaaaca gcggcgaaac ggcggcttgg
     6841 cggcagtggc ggcggtttct cgaacaggcg gattcctact ccgttttaaa ggatttcccc
     6901 tcgcgcaaac acacttcgct gatgggcgcg tatttgagtg ccggctgcat ctcgccgcgc
     6961 ctgctcgcgc gggaaagcct cgaacgccgt ctgaacgcgt gggcggacaa catcatccgc
     7021 cgcgattttt tccttcaact tgccttgcag cacacggatg acgacccttc agacggcaat
     7081 cctgaacaca ccctgcgcct gacgctttgg cagcagggcc ggaccggcat tccgattatc
     7141 gatgccgcga tgcgctgttt gcacaaaacc ggcagcctcc accccgccct gagacgcttg
     7201 agcgcggatt ttttctgcca cgttttaaac ctcccccgcc gcgaaggcga gatatggttt
     7261 gcccggcagc tgaccgattt cgatgcggca atcaaccaag gcaactggtg gcttgccgcc
     7321 tcacggcaca cctgccccga cattgccgcc gcctcatacc gtaccgaccc ggacggtact
     7381 tttatcaaac ggcacattcc cgaactcgcc cacctgtccg ccgacaccgt ccacacgcct
     7441 tggcggtttg cctgttcggt cgatacccac ggctatcccg cccatcctgt cgccggtatt
     7501 ccctaagcgg tacggcaacc taaaacaaat gccgtctgaa cttccgtttc agacggcatt
     7561 gcgtccgaat gactcaatat cttccgtttt tttcactttc aatcatcagg tatgcccaaa
     7621 tcaccgccac ccaagccgac accgttccca tcgccggcaa aaaatgctgc caatccgtca
     7681 tcggcggcac agggcaacgg ccgcattcga tttccgcgcc gcccaacaac cggtacagca
     7741 acatatccgc aatccaaagc aacaacgggt atttcaaacg ctgcaaccaa aaaaccaacc
     7801 acgtccgcac gattcacctc accccccatt ccgacaacga tattgtaccg cttccgtgca
     7861 atgttaaaat aagcccactt ctaccgccgt acaaaacgcc atgctcaccg atttagaaaa
     7921 aaacgccatc cgcgaccatt accaaaacat cggcaaaaac ctgcccggtt tccgtccgcg
     7981 cgcttcgcag cgggaaatga ttgcggcggt tgccaacgct ttttcgcgga cgttggcgcg
     8041 cgaagaaggc ggcgagccgc ccaagcgcga gggcgagagc atcgccgtaa tcgaagggcc
     8101 gaccggcgtg ggcaaatcgc ttgcctacct gctggcgggc ggcatcatgg cgcagacgcg
     8161 cggcaagcgg ctgattgtga gcagcgcaac ggttgccttg caggagcagt tggtagaccg
     8221 cgacctgccg tttttggtcg aaaaaagcgg tttggaactg agcttcgcgc ttgccaaagg
     8281 gcgcggccgc tatctctgcc cctacaaact ctatcaactg acgcaaagca atgcccagca
     8341 aaacctgctc ggctttgaag cccccgccgt cttgtgggac agcaaaccca agcccgaaga
     8401 attgaagctg ctgcgcgaca ttgccgacga attttctgcc cgacggttca atggcgaccg
     8461 cgacgcctgg ccggaaaaaa tcgacgacgc gatttggctc aaagtgacca acgaccgcca
     8521 cggctgcctg aaaaccgcct gtcccaaccg tccggaatgt ccgttttacc tggcacgcga
     8581 tgtcttggaa accgtcgatg tcgtcgttgc caaccacgat cttctgcttg ccgacatcag
     8641 tatgggcggc ggcgtgattc tgcctgcgcc cgaaaacagt ttctattgca tagacgaagc
     8701 gcaccacctg cccaaaaaag ccctcagccg ttttgccgcc gaacattcat ggaatattgc
     8761 cgtttggacg ctggaaaaac tgccgcagct gaccggcaaa attgccgcgc ttaccgataa
     8821 agccgaactt gccaacctgg ccgacgaagc cgccgcatcc ttgctcgaca gcctgcacga
     8881 atggcagttc catttggcgg aagagccgtc tttaagtatg gggccgtctg aaaacgacag
     8941 acgaaccaac agcgaaccga cttggctgtg ggaagacggc aaaatccccg aaggcctcga
     9001 aaccaccgtt tccaatacgg ccattgctgc gcgcagcctg ctcaaacacg ttgtcgggct
     9061 gaacgatgcg ctttccgccg cgcgccgcga aaaagaacag gacggcgcgc tcctcgaccg
     9121 cctgaccggc gagttcggca tcttcatcgt ccgtatcgaa caaatcagcg cggtttggga
     9181 tttgctctcc accgtccccc tcgaaggcga agagccgctg gcaaaatgga taacccgccg
     9241 tgccgacgac aaaaacgact acattttcaa cgccagcccc atcagcagcg catcccacct
     9301 tgccaacagc ctgtggcggc gtgcggcagg cgcggtattg acttccgcca ccctgcaatc
     9361 cttgggcaac ttcaacctga tgctgcgcca aaccgggctg caatggctgc ccgaaaccac
     9421 caccctcgcc ctcaaaagcc cctttgactt tgaaaaacag ggcgaactct acatcccccc
     9481 catatacgcc agccccaaag accccgaagc ccacaccgcc gccgtcatcg aatggctgcc
     9541 caagcttatt tcgcccaccg aagccatcgg cacgctcgtc ttgttttcct cgcgcaaaca
     9601 aatgcaggat gtcgccctgc gcctgcccgg agactacctg ccgctcttgc tcgtacaagg
     9661 cgaattaccc aaagccgtcc tcctgcaaaa acaccaccgg gccatagaag aaggcaaagc
     9721 cagcatcatc ttcggactcg acagtttcgc cgaaggactc gacctgcccg gcaccgcctg
     9781 cgtgcaagtc atcatcgcca agctgccgtt tgcgatgcct gacaacccca tcgaaaaaac
     9841 ccaaaaccgc tggatagaac agcgcggcgg caatcctttt attgaaatca ccgtccccga
     9901 agccggcatc aaactcatcc aggccgtcgg ccgcctcatc cgcaccgaac aagactacgg
     9961 ccgcgtaacc atcctcgaca accgcatcaa aacacagcgg tacggccaac aattattggc
    10021 cggcctgccg ccgtttaaaa ggatagggta aacataccgc ccttcccgca acagaaagga
    10081 aaaattcatg aactttaccc gactgctcaa ccaagtctta agcacggttc aaaaaaaagg
    10141 cggcacattc tccgacagcc cgctcaattc attcggcgga ggcgcgctgg ttgccggtat
    10201 cgcctccatg ctgctgaacg gtaaaaaccg caaaaccatt accaaaatcg gttcgaccgc
    10261 cgctttgggc tacctcgcct accggggcta tcagatgtgg cagcaaaaca aagggcgggc
    10321 aactgtaaca caaagcgatt tccaacctgc cggagaaact gaagaaacat acagccgcac
    10381 cgtattgcgc accatgatag ccgccgccgc ttcagacggc atgatagacg aagccaaacg
    10441 ccggactatc gaacaggaaa gcggcacaga ccccgaaact gccgcatggc tcgccgccga
    10501 ataccgcctg cccgcaagca tcgaagacat cgccgccgcc gtcggcaacg atgaggcgtt
    10561 ggcggcggag gcctatctgg cggcaaggtt ggtctgtgcc gatttgtcgc ggaaagaaac
    10621 cgtcttcctc gcccgcctgt cgcaggcttt gaaactggat gacaatctgg tggagagttt
    10681 ggaaaggcaa ttggggtttt gatgacacag ccggggaggg aaaatcatca aaaaacggtt
    10741 attccgaccc cggcctcaaa acggcaa
//
LOCUS       DAXHSJ010000054        10466 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000054 DAXHSJ010000000
VERSION     DAXHSJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.054"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            84..1049
                     /locus_tag="ACFM8U_001028"
     CDS             84..1049
                     /locus_tag="ACFM8U_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6334284.1"
                     /translation="MLRLTALTLCTVLALGACSPKDSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLNKLGVKTGLSIDKNRLPYLDEYYKTTKPA
                     GTLFEPDYEALNAYKPQLIIIGSRAAKAFDKLNAIAPTIEMTADTANLKESSKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1214..2182
                     /locus_tag="ACFM8U_001029"
     CDS             1214..2182
                     /locus_tag="ACFM8U_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6334285.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMVGASQSAALGLLLMTLLL
                     PAAPLLAKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVIEAVATFIAY
                     ENEMLQMLGVWQQGDFSGVLLGRYELLWATGILALFAYLIADQLTILGLGETVSVNLG
                     LNRTAILWSGLIIVALITSLVVVTVGNIPFIGLVVPNIISRLMGDKLRQSLPAVALLG
                     ASLVLLCDIVGRVIVFPFEIPVSTVFGVMGTALFLWLLLRKPAHAV"
     gene            2172..3146
                     /locus_tag="ACFM8U_001030"
     CDS             2172..3146
                     /locus_tag="ACFM8U_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC6334286.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHLLGMQAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3191..3817
                     /locus_tag="ACFM8U_001031"
     CDS             3191..3817
                     /locus_tag="ACFM8U_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC6334287.1"
                     /translation="MAQEHFPSFFNQAPTITVRDALAEFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGAELPQRGDIEAFMQGGRDEGTTGVTASVVQLLTG
                     AAPETGFGGVGPAGRFARRHLLSFGAGEINGTLALRRRDTGKTVAVSLNAALQPFAPE
                     MRELMPKAVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3899..4657
                     /locus_tag="ACFM8U_001032"
     CDS             3899..4657
                     /locus_tag="ACFM8U_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6334288.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKAVLTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(4716..5054)
                     /locus_tag="ACFM8U_001033"
     CDS             complement(4716..5054)
                     /locus_tag="ACFM8U_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC6334289.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            5149..5361
                     /locus_tag="ACFM8U_001034"
     CDS             5149..5361
                     /locus_tag="ACFM8U_001034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334290.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRAETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            5518..9474
                     /gene="purL"
                     /locus_tag="ACFM8U_001035"
     CDS             5518..9474
                     /gene="purL"
                     /locus_tag="ACFM8U_001035"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC6334291.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKTLDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAANIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVEDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHHPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQQDRDYVDAAFKAAD
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNGRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRIHLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPDTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKSAS
                     LILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG"
     gene            9569..10327
                     /gene="gloB"
                     /locus_tag="ACFM8U_001036"
     CDS             9569..10327
                     /gene="gloB"
                     /locus_tag="ACFM8U_001036"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC6334292.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGEGLITVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAEHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
BASE COUNT         2380 a         3138 c         2697 g         2251 t
ORIGIN      
        1 attatcattt atccttttct aaagccgttc cggtttgtcc gaccggcggc tttgccccaa
       61 tatccccatt ttggagacac cctatgttgc gtttgaccgc attgaccctc tgcaccgtcc
      121 tcgccttggg cgcgtgttcg ccgaaagatt ccgactctgc cccacaagcc aaagaacagg
      181 cagtttccgc cgcacaatcc gaaagcgcgt ccgttaccgt caaaaccgcg cgcggcgatg
      241 ttcaaatacc gcaaaacccc gaacgtatcg ccgtttacga tttgggtatg ctcgacactt
      301 taaacaaatt gggagtgaaa accggtttat ccatcgataa aaaccggctg ccttatttgg
      361 acgaatatta caaaaccacg aaacctgccg gaactttgtt cgagccggat tacgaagcct
      421 taaacgccta caagccgcag ctcatcatca tcggcagccg cgcagccaaa gcgtttgaca
      481 agttgaacgc cattgcgccg accatcgaga tgaccgccga taccgccaac ctgaaagaaa
      541 gctcaaaaga gcgtatcgac gcgctggcgc aaatcttcgg caaacaggcg gaagccgaca
      601 agctgaaggc ggaaatcgac gcgtcttttg aagccgcgaa aaccgctgcg caaggcaaag
      661 gcaaaggttt ggtgattttg gtcaacggcg gcaagatgtc cgccttcggc ccgtcttcac
      721 gactgggcgg ctggctgcac aaagacatcg gcgttcccgc tgtcgatgaa tcgattaaag
      781 aaggcagcca cggtcagcct atcagctttg aatacctgaa agagaaaaat cccgactggc
      841 tgtttgtttt agaccgcagc gcggccatcg gcgaagaagg tcaggcggcg aaagacgtgt
      901 tgaataatcc gctggttgcc gaaacaaccg cctggaaaaa aggacaggtc gtgtacctcg
      961 ttcctgaaac ttatttggcg gccggcggcg cgcaagagct gctgaatgca agcaaacagg
     1021 ttgcggatgc ttttaacgcc gcaaaataat gaaacggcgg cattcgatgc cgtctgaaac
     1081 acggatgcaa accgcctcct gggtttcaga cggcatttgc ccgatacgga ggtttcaaac
     1141 aaggctttcc gctccgacgg ttcggactgc cttgtttgaa tcttctacgc cttaacgctt
     1201 ttcccttctg tttatgactg ccaaaccttt ttccctcaac ctgaccaacc tcctgctgct
     1261 ggcggtgttg tttgccgtca gcctgtcggt cggcgttgcc gatttccgct ggtcggatgt
     1321 gttttcgcta tccgacagcc agcaggttat gttcatcagc cgcctgccgc gcacgtttgc
     1381 gattgtgctg acgggcgcgt cgatggcagt ggccggcatg attatgcaga ttttgatgcg
     1441 caaccgtttt gtcgaaccgt cgatggtggg cgcaagccaa agcgcggctt tgggtttgct
     1501 gttgatgacc ctgctgctgc cggccgcgcc gctgctggca aaaatgtcgg ttgccgccgt
     1561 tgccgcgctg atcgggatgt tggtctttat gttgctgatc cgccgcctgc cgccgaccgc
     1621 gcaactgatg gtgcctttgg tcgggattat tttcggcggt gtgattgagg cggtggccac
     1681 ctttatcgcg tatgaaaacg aaatgctgca aatgctcggc gtgtggcagc agggcgattt
     1741 ttccggcgtg ttgctcggac gctatgagct gttgtgggca acgggaattt tggctttgtt
     1801 tgcctatttg attgccgacc aactgacgat tttgggtttg ggtgaaacgg tgagcgtgaa
     1861 cttggggctg aaccggacgg cgattctgtg gtcggggctg attattgtgg cgctgattac
     1921 gtctttagtg gtcgtgacgg tcggcaatat tccgtttatc ggcctagtcg tgccgaacat
     1981 catcagccgc ctgatgggcg acaagttgcg tcaaagcctg cctgcggtgg ccttgctggg
     2041 cgcatctttg gtgttgctgt gcgatattgt cggacgcgtg attgtgtttc cgtttgaaat
     2101 tccggtctct acggtttttg gtgtgatggg gacggctttg tttttgtggc ttttattaag
     2161 gaaacctgct catgccgtct gaaaaaaata tcggttttat ggcaggaagc agccgtccgt
     2221 tgtgggttgc ctttgcgctg ttgctggttt cctgcatctt gtttatgacg ctcaacgtca
     2281 aaggcgattg ggattttgtc ttgcacctgc gcctgaccaa acttgccgcg ctgctgatgg
     2341 tcgcctatgc ggtcggcgtg tccacgcaac tcttccaaac gctgaccaac aatccgattc
     2401 tgaccccttc gattttgggt ttcgattcgc tatatgtgtt tttgcagacc ttgctggtgt
     2461 ttacgttcgg cggcgtgggc tatgcttccc tgccgttgac gggcaaattc ggctttgaac
     2521 tggttgttat gatgggcggt tcgctgctgt tgttctacac gcttatcaaa cagggcggac
     2581 gagatttgtc gcgcatgatt ttaatcggcg tgattttcgg gattttgttc cgcagcctgt
     2641 cgtcgttgct ttcgcgcatg attgaccccg aagaatttac cgccgcgcag gcgaatatgt
     2701 ttgccggatt caataccgtc cacagcgagc ttttgggcat aggcgcgctg attctgctcg
     2761 tcagcgcggc agcggtttgg cgcgaacgct accgcttgga cgtacacctt ttggggcgcg
     2821 accaagccgt caatttgggc atcagctaca cgcgcaacac cttgtggata ctgctttgga
     2881 ttgccgcgct ggtggcgacg gcgaccgccg tggtcggccc ggtaagcttt ttcgggcttc
     2941 tcgccgcctc gcttgccaac cacttttccc cgtccgtgcg ccattccgtc cgcctgccga
     3001 tgacggtttg tgtcggcggt attcttctgg tcggcggaca gaccgtattc gaacacctgc
     3061 tcggtatgca ggcagtgttg agcgtggtag tagaatttgc gggcggactc gttttcctct
     3121 atctcgtttt aaaacacaaa aaatgacgga tgccgtctga acggccgtcc gcctcgacag
     3181 gacaaaacat atggcacaag aacatttccc atcattcttc aaccaagccc cgaccatcac
     3241 cgtcagggac gcgcttgccg aattcctcgg cgcggccgaa aacggcatcc tcacttaccg
     3301 ctacgccgat gccgtgcgcc tgtgcggaca ttcctgcccg accgtcgcgg gcgcgtacct
     3361 gatggttatc aaaggtctga aagcccttta cggcgcagag ctgccgcaac gcggcgacat
     3421 cgaagccttt atgcagggcg ggcgagacga aggcacgacc ggcgttaccg cttccgtcgt
     3481 ccaactcctg accggcgcgg cacccgaaac cggctttggc ggcgtaggcc cggccggacg
     3541 ctttgcccgc cgccacctct tgtcctttgg tgcaggcgaa atcaacggca cactcgcgct
     3601 ccgccgccgc gataccggca aaaccgttgc cgtcagcctc aacgccgccc tgcaaccctt
     3661 tgcacccgaa atgcgcgaac ttatgcccaa agccgtcggc ggcagcgcaa gcgcagaaga
     3721 actcgaacgc ttcggacaac tctggcaggc gcgcgttaaa gcatttttga ccgaatcggc
     3781 ggacgacccg cagttcgtca tcgtccgcga agtgtgagcg ttcagacggc attccgaatt
     3841 tcaaatgccg tctgaacccc cgccaaacaa caaacctacg cccgacaagc atccgcctat
     3901 gattaccatc cgcaacgtca gctaccgcat cggcacacgc cccatcctcg acaacgtcag
     3961 cctcgacatc cccgaaggcg gcattaccgc cctcgtcggc cccaacggtg cgggcaaatc
     4021 caccctgttt tcctttatgg cgcggctgcg cccgcttgaa agcggcagca tcgcctaccg
     4081 cggcaaaaac cttgccggca cccccaccgc cgaactcgcc cgcacgctct ccatcctcac
     4141 ccaagaaaac agcatcatga gccgcatcac cgtgcgcgac ctgctgatgt tcggccgtta
     4201 cccctaccat caaggcagac cgactgccga atgccgccgt atcgttaacg gtgcaatcga
     4261 agaattccac ctgcaagacc tctccgaccg ctatctgacc gagctctccg gcggccaacg
     4321 ccaacgcgcc atgattgcga tggtgttctg ccaaagcacc gactacgtcc ttttggacga
     4381 accgctgaac aacctcgata tgtatcacgc ccgctcgctc atgcagattc tgcaaaagct
     4441 cacgcgcgaa cacaaacgca ccaccgtcgc cgtattgcac gacattaacc aggccgcagc
     4501 ctacgccgac cacgtcgtcg ccatgaaaaa cggcaaagcc gtcctgaccg gcacgcccga
     4561 agaagtgttc accgtacaca acatcaaaga actgttcgac atggatgtgg acgtactcga
     4621 ctacgaaggc aaaaaactca tcgtccacca tatctgaagc tcaaaaagaa aatgccgtct
     4681 gaaaaccttt cagacggcat ttccattcca aacgctcaaa ccgccgcgtc cgaacgttcg
     4741 cccgtgcgga tacggattgc ctcctcgacc ggcagcacaa aaatcttgcc gtcgccgatt
     4801 ttgcccgaac gcgccacctc gacaatcacg tcaatcgcgc gttccacagc gtcatccgcc
     4861 aacaccagct cgattttgac cttgggcagg aaatcgacgg cgtattccgc accgcgatag
     4921 atttccgtat gccccttctg cctgccgaac cctttgacct cgctgacggt catccccgta
     4981 atgccgattt ccgtcaacgc ctcgcgcacg tcgtcgagtt tgaacggttt gacaatcgcc
     5041 tcgatttttt tcatcacatt ctccattaat taacgtaaac aaacgacaaa tatagcagaa
     5101 accgccggca aacgataaac ggtactgttc gcccggcgat gcggcgattt gccggaaacc
     5161 gtcattcccg cgcaggcggg aatcccgatc tctgcatttc agcaatattt aaaggtttct
     5221 gtaaatccaa acttccatat tcctaaccgt gcggaaacga cggcatttga tctaaaaaca
     5281 tacggaagca gttgcccgcc cctctacttc ctgcaattat ccgcataccc ggctttgggt
     5341 aaaaatcaga aaaacagata aaacccttat tccttaatat cttcagacgg cacctgtatt
     5401 ttccccaact gtatgccgtc tgaagattgt caacatttta agccaaaata tccaagcaat
     5461 acaaacctgt ccggcgtata atccctcttt ttcccaaccg catttgagag ccgaattatg
     5521 tctgttgtgt tgcccttgcg cggcgttacc gccctttccg atttccgtgt tgaaaaactc
     5581 ttgcaaaaag ccgccgcact cggtctgccc gaagtcaaat taagcagcga attttggtat
     5641 ttcgtcggca gcgagaaaac actggatgcc gcaactgtcg aaaaactgca agccttactg
     5701 gcagcgcaaa gcgttgaaca aacgcccgaa gcgcgcgagg gcttgcattt atttttggtc
     5761 acgccccgtt tgggtacgat ttcgccttgg gcttccaagg caaccaatat cgcggaaaac
     5821 tgcggtttgg caggcatcga gcgcatcgag cgcggcatgg cggtgtggct ggaaggtgcg
     5881 cttaccgatg aacagaaaca gcaatgggcg gctttgctgc acgaccgcat gaccgaatcc
     5941 gtattgcccg attttcagac ggcatccaaa ttattccacc atctcgaatc cgaaaccttt
     6001 tccaccgttg atgttttagg cggcggtaaa gaggctttgg tcaaagccaa taccgaaatg
     6061 ggtttggcac tctccgccga cgaaatcgat tatctggttg aaaactatca ggctttgcag
     6121 cgcaatccgt ccgatgttga attgatgatg ttcgcgcagg caaacagcga acactgccgc
     6181 cacaaaatct tcaacgccga tttcatcctc aacggcgaaa aacaaccgaa atccctgttc
     6241 ggcatgatac gcgacacgca caacgcgcat cccgaaggta cggtcgtcgc ttataaagac
     6301 aattcgtccg taatcgaagg cgcgaaaatc gagcgtttct atccggatgc ggcggaaaac
     6361 caaggctacc gtttccacga ggaagacacg catatcatca tgaaagtgga aacgcacaac
     6421 cacccgaccg ccatcgcgcc gtttgcgggc gcggcgacgg gcgcgggcgg cgaaatccgc
     6481 gacgaaggcg caacgggcaa aggttcgcgt ccgaaagcag gcttgaccgg ctttaccgtg
     6541 tccaacctca acatccccgg cctcaaacag ccgtgggaac aagattacgg caagccggaa
     6601 catatttcct cgccgctgga cattatgatt gaaggcccca tcggcggcgc ggcgttcaac
     6661 aacgaattcg gccgccccaa cctcttgggc tacttccgca cctttgaaga aaaatttgac
     6721 ggtcaggttc gcggctatca caaaccgatt atgattgccg gcggtctggg cagcattcag
     6781 gcgcagcaga cgcataaaga cgaaatcccc gaaggcgcgt tgctgatcca actgggcggc
     6841 ccgggtatgc ttatcggctt gggcggcggc gcggcttctt cgatggatac cggtacaaat
     6901 gacgcgtctt tggacttcaa ctccgtgcaa cgcggcaacc ccgaaatcga acgccgcgcg
     6961 caggaagtga tcgaccgctg ctggcagctc ggcggcaaaa acccgattat ctccatccac
     7021 gacgtgggcg cgggcggttt gtccaacgcc ttccccgaac tcgtcaacga tgccggacgc
     7081 ggcgcggtat tcaagctgcg cgaagtgccg ttggaagaac acggtttaac gcctttgcaa
     7141 atctggtgta acgaatcgca agagcgttat gtgttgtcga ttttggaaaa agatttggac
     7201 accttccgcg ccatctgcga acgcgaacgc tgcccgtttg ccgtggtcgg cacggcgacc
     7261 gacgacggcc atttgaaagt gcgcgacgat ttgttcgcca acaaccccgt cgatttgccg
     7321 ttgaacgtct tgctcggcaa accgcccaaa accacgcgta ccgacaaaac agttacgcca
     7381 tctaaaaaac cgtttcacgc cgacgatatc gacatcaccg aagccgccta ccgcgttttg
     7441 cgcctgcctg ccgtagccgc caaaaacttc ctgattacca tcggcgaccg cagcgtcggc
     7501 ggcatgaccc accgcgacca aatggtcggc aaataccaaa cccccgtagc cgactgcgcc
     7561 gttaccatga tgggcttcaa tacctatcgc ggcgaagcaa tgtctatggg cgaaaaaccg
     7621 accgttgccc tgtttgacgc gcccgcctcg ggcagaatgt gcgtcggcga agccatcacc
     7681 aacatcgcgg cagccaatat cggcgacatc ggcaacatca agctctccgc caactggatg
     7741 gcggcgtgcg gcaacgaagg cgaagacgaa aaactctacc gcaccgtcga agcggtttcc
     7801 aaagcctgtc aggcattgga tttgagcatc cccgtgggca aagacagcct gtcgatgaaa
     7861 actgtgtggc aggacggcga agagaaaaaa tccgtggttt cgcccttgag cctgattatc
     7921 tccgcgtttg ctccagtgga agacgtacgc aagactgtta cacccgagtt gaaaaacgtc
     7981 gaagacagcg tattgctgtt tgtcgatttg ggcttcggca aagcgcgtat gggcggctcg
     8041 gcgttcggtc aggtgtacaa caatatgagc ggcgacgcgc ccgatttgga cgacacaggc
     8101 cgtctgaaag cgttttacaa cgtgattcaa cagcttgtcg ccgaagacaa acttttggcg
     8161 tatcacgacc gcagcgacgg cggtttgttt gcgacactgg cggaaatggc gtttgcggca
     8221 cggtgcggta tcagtgccga tatagattgc ctgatggata aattcctgcc gattcatcac
     8281 ccggatttcc aaggcgaccc ggccgaggac ttatctgacg aactttataa tcatgccgcc
     8341 attaaaatct tattcaatga agaattaggt gcggttatcc aaatccgcca acaagatagg
     8401 gattatgttg atgcggcatt taaagcggcc gacttaaccg atgcggtcag ccggattggc
     8461 tctcctgatt ttgacaatga gtttatttct ttctttggtt acggctattt cttggaacaa
     8521 aaccgcgccg acctgcaacg cgcatggcag gaaaccagcc acgccatcca acgcctgcgc
     8581 gacaaccctg cctgcgccga cagcgagttc gcattgattg gcgacaacgg acgcagcgca
     8641 ttgtttgcca acctgaaatt cgacgtgaac gaagacatcg ccgcgccgtt tatcaacagc
     8701 ggcgcgaaac ccaaaatcgc catcctgcgc gaacagggcg taaacgggca aatcgaaatg
     8761 gccgccgcct ttacccgcgc cggattcgat gcttacgacg tgcatatgtc cgacctgatg
     8821 gcaggccgca tccacctcgc cgacttcaaa atgctggcgg cgtgcggcgg cttcagctac
     8881 ggcgacgtac tcggcgcggg cgaaggctgg gcgaaatcga ttctgttcca ccctgctctg
     8941 cgcgaccagt ttgccgcctt cttcgccgac ccggacacgc tgacattggg cgtgtgcaac
     9001 ggctgccaaa tggtcagcaa ccttgccgaa atcatccccg gcacggcagg ctggccgaag
     9061 ttcaaacgca acctgagcga acagtttgaa gcgcgcctaa gcatggttca cgtccccaaa
     9121 tccgcgtcgc tgattctgaa cgaaatgcaa ggctccagcc tgcccgtcgt cgtcagccac
     9181 ggcgaaggcc gcgccgactt cgcacttcac ggcggcaaca tttctgccga tttgggcatt
     9241 gcgctgcaat atgtggacgg tcaaaaccaa gttacccaaa cctatccgct caaccccaac
     9301 ggctcgccgc aaggcatcgc cggcattacc aacgccgacg gccgcgtgac catcatgatg
     9361 ccccaccccg aacgcgtgta ccgcgccgca caaatgagct ggaaaccgga aggctggacg
     9421 gaactgtccg gctggtaccg cctctttgcc ggcgcacgta aagccttggg ctaaccgccc
     9481 tactcaaacc aatgccgcct gaagaatatt tcagacggcg ttccggcata ccatccttta
     9541 aacggtatcc gtccaccgag gaacactcat gaaaatcacc cccgtcaaag ccctaaccga
     9601 caactacatc tggatgatac agcacggcaa ccatgccgtc tgcgtcgacc cttccgaacc
     9661 ctcgcccgtc ttggaattcc tcgtccgcaa ccgcctcatg cttgcccaaa catgggtaac
     9721 gcacccccat cccgaccacg agggcggtgc ggcggcactc tggcgcggct acatggaatc
     9781 gcccgtttac ggcgaatccg acatcgaagc agcaacccac accgtaaccg ccggcaccca
     9841 attcaccttc ggcgaagggt taatcaccgt ttgggcaacc ccgggccaca cagaccgcca
     9901 caccagctac cttctcgaaa cttcagacgg catacacgtc ttttgcggcg acaccctttt
     9961 ttccgccggc tgcggacgcg tgtttaccgg cacaatcgaa cagctttacg acagcttcca
    10021 acgcttcaac caattacccg aaggcaccct gttctacccg gcgcacgaat acaccgccgc
    10081 caacctgcgt ttcgccgccc atatcgagcc ggacaacgcc gacattcaga cggcactgaa
    10141 agcggcagaa cacacgccta ccctgcccgt taccctcgcg cacgaacgcc gcgtcaaccc
    10201 gtttttgcgc gtcgacctgc cgcacgtcag agaccgcgcc gaggcattga gcgggaaaac
    10261 gttaaacagc agcctcgata cctttgtcgc gctgcgtgaa cttaaaaacc aataccggac
    10321 gaaataaaac aacgggaaaa cgcagccatt cctaggattt ttattaaaat cttaaataaa
    10381 atcatacaat catcgccaat agacgaaagg acaccgttgc cttataatca aacaaaaaca
    10441 aaatatataa tatagtggat tgaatt
//
LOCUS       DAXHSJ010000055        10432 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000055 DAXHSJ010000000
VERSION     DAXHSJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.055"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            154..1038
                     /locus_tag="ACFM8U_001037"
     CDS             154..1038
                     /locus_tag="ACFM8U_001037"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC6334293.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLESGIREKISSHLARGKVECKIQIQDTENGNQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTDALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIGALSEIFPALVEAHKEKIRARLAEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(1122..1637)
                     /locus_tag="ACFM8U_001038"
     CDS             complement(1122..1637)
                     /locus_tag="ACFM8U_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6334294.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(1712..2131)
                     /locus_tag="ACFM8U_001039"
     CDS             complement(1712..2131)
                     /locus_tag="ACFM8U_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334295.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAIIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(2252..3250)
                     /gene="holA"
                     /locus_tag="ACFM8U_001040"
     CDS             complement(2252..3250)
                     /gene="holA"
                     /locus_tag="ACFM8U_001040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC6334296.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEINKLGLLYPKGHTVNIDQAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGEKQTLAPIAVRRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            complement(3252..3731)
                     /gene="lptE"
                     /locus_tag="ACFM8U_001041"
     CDS             complement(3252..3731)
                     /gene="lptE"
                     /locus_tag="ACFM8U_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC6334297.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(3783..4562)
                     /gene="pgeF"
                     /locus_tag="ACFM8U_001042"
     CDS             complement(3783..4562)
                     /gene="pgeF"
                     /locus_tag="ACFM8U_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC6334298.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVLRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTTVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            4715..4939
                     /locus_tag="ACFM8U_001043"
     CDS             4715..4939
                     /locus_tag="ACFM8U_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334299.1"
                     /translation="MMPLYTFSELYMFQQGMAHQAPHCVLPERDYPPIRFYRYKQMGF
                     NRKGMGIKSISDISRAMPSENQSPLSDGIV"
     gene            5016..5963
                     /locus_tag="ACFM8U_001044"
     CDS             5016..5963
                     /locus_tag="ACFM8U_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6334300.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMAYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLTIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(6116..7240)
                     /gene="rluD"
                     /locus_tag="ACFM8U_001045"
     CDS             complement(6116..7240)
                     /gene="rluD"
                     /locus_tag="ACFM8U_001045"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC6334301.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIIYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            7239..8042
                     /locus_tag="ACFM8U_001046"
     CDS             7239..8042
                     /locus_tag="ACFM8U_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC6334302.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(8117..10336)
                     /locus_tag="ACFM8U_001047"
     CDS             complement(8117..10336)
                     /locus_tag="ACFM8U_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC6334303.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRVWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTAVRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     SGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         2453 a         3139 c         2634 g         2206 t
ORIGIN      
        1 gttaatccac tatacatttc ccttccttgc agcaacaaaa gcgcgtgatt ccaatctgcc
       61 gctgcaaaat cttcaatttt tcacacccga aaacgcttgc cgccatacag atgtgatata
      121 ttgattccat tttaacgggc ttcggagaac gacatgagca gcggaaatat ccacatccac
      181 agcatgaccg gctttgccaa cgcggcggcg gaatgcggca gcaaacgcat caacctcgac
      241 atccgcgccg tcaaccaccg ctttttggac attcaaatca ggatgccgga tgatttgcgc
      301 catttggaaa gcggtatccg cgagaaaatt tcatcccatc tggcacgcgg caaagtcgaa
      361 tgcaaaatcc aaattcaaga tacggaaaac ggcaatcagt ctttggaact gaatcgcgat
      421 ctggtcggac agcttgccga aatcaacaaa gacctccgca agcatcacga tttggcaaaa
      481 ctgggcgttg ccgacatcct gcgtttcccc ggcgtattgg caagccaaag ggaaaatacg
      541 gacgcattgg caaaggttat tgccgatttg accgattcgg cactgaaaga ctttaccgcc
      601 gcacgccgac gcgaagggaa aaaattgggc gaacacctgc tgcaacgcct cgagtctatg
      661 gaagaaatca tcggggcatt gagcgaaatc ttccccgcgc tagtggaagc gcataaagaa
      721 aaaatccgcg cccgccttgc cgaagccgtc ggcgacatca ataatgacag gctgcaacag
      781 gaatttgccc tctttattca aaaatccgac atcgacgaag aattcagccg cctgcgcacc
      841 cacattgccg aagtgcgccg tatcgttacc gaacacaaag gcagcagcgg caaacggctg
      901 gacttcctga tgcaggaatt aaaccgcgaa gccaacactt tgggcagcaa atccattgcc
      961 gccgaatgca cccaagcctc ggtcgaactg aaagtcttaa tcgagcagat gcgggaacaa
     1021 gtgcagaata tcgaataatc ggtttccccg ataagcaaat gccgtctgaa agccctttca
     1081 gacggcattt tttcaggttg aaacctttgc ggtataaagg cttaaggctt tcctttgacc
     1141 gccaccaatc ttgttcctgc aagaaaatca tacagaaact gccgatcggg attcagcagt
     1201 gcgaaccccc acggtaaaat cagccaaatc aatgccgcgc cggccgcgcc cttgggcgga
     1261 atgccgagga agtggcgtaa tccggcatag gctaacatag ggataaatac gataaatatg
     1321 cacgcccaaa taaagcgcag gcgcagcagg tgcaaagacg gctgtatgcc gttaaggtcg
     1381 cacaagccga ttttccatgt cctcatcgcc aaggtctgcc cctgaccatg ccagttggcg
     1441 cggaaataaa gccaccatgc tcccattatc aggatacttg ttaccaatgc agaaaccgcg
     1501 atagaaacgg gattcagaaa aatggcggca ataccggcaa acaatgctgc caaacaggtt
     1561 gccgcaccga ccagcagcat ttcgtacagc atggcggcaa agcggcgttt gagcggggcg
     1621 gttttcagta tcatcatctt ttcaatcgga atatgccgtc tgaaacgcca acaggcttca
     1681 gacggcattc atgcagatat gtctcatgtg gttaacggtg cggacgtttc tgaccacgcg
     1741 actgttttgc cgccatttga ttgatttcct gacgtgtcgg gcgttttttg aacatagcgt
     1801 gcgcctcgtc caccgaacat ttttgcgaca ggcagatcag ggtaatcgca tctttctggt
     1861 gttgtccgcc gctgtggtac atacggcgca ggtttgcctc gttgattctg cctttttcgg
     1921 gatcgatgtc gaattttttc aggcgttcga taaactggcg ttgctgtcgg cgggatgtcc
     1981 aaacaaaacc gcccgcagcg gcagcgacaa tgatggcggc aagcaggatg gcaagcggcc
     2041 aggaatcgac aatcaatacg ataatgaggc ttaaaacggc ggtggtcagt aaaatcaggc
     2101 cgcccaagcg cagtttggtc ttattgtcca tagggttaat gttcctttat tgaggtggtc
     2161 gtgccgtctg aaatgttttc ggacggattg gtgaatactt ttcaggatta acggtatttt
     2221 acagtatttt cggattttgg ggattaccgc tttattccgc cagcgacacg acaagccgtt
     2281 taaataccgt ccatgcgtcg ccgtcttccg cacctttgat gattcggtcg atttgggcgc
     2341 aggttttgag cgcgtcaagc aggcggacga cggaaatccg tctgaccgca atcggagcga
     2401 gcgtctgctt ttcaccccaa agcctgaggc tgttgcggac ggattggatg ctctgcccct
     2461 gcttcagggc ggcggcaagc cggatcagcg tccgcacgtc ttcggcaacc gcccacagca
     2521 acagcaccgg ttcttcgcct tcctcctgca atccgtccaa caatctgcat acgcgcggga
     2581 catcgccctt catccacgcg cctgccagtt ggaacgcgtc aaagcgggcg acgttggcaa
     2641 cggcggtttg cgcctgatcg atattgacgg tatgcccttt cggatacagc agcccgagct
     2701 tgttgatttc ctgacgcgcc gccaacaaat tgccttccac gcgctcggca aacagtgcca
     2761 atgcgtctgc ctcgataccc aaaccgattt tgtccagccg tccgcgtatc cattggggca
     2821 aagccgccgc gccgaccggt ttggcttccc acacttcccc ctttgccgcc aatgcggcaa
     2881 accatttgga ctggagctgg gttttctcca gtttgggcag caaaaccagc gttaccgtat
     2941 cttccggcag gcgggcggca aaatcctgca acgcctcgcc gccggttttg ccgggttttc
     3001 cgttggggat atgcagttcc aacagcttca aatcggcaaa cagacccgca ctgcctgcgg
     3061 tttgcaacag ttcgttccaa tcgaaggcat tgtctgccgt ataaacttcc cgattaaggt
     3121 aaccttgttt cttcgccgcc gccctcaatg cgtccaatgc ctcgatacgc aacagttctt
     3181 cctcgccgtg gatgacgtac agaggtttca aaggcgcgtc cgtatcaatg cgtccgatat
     3241 gtgccgccac gtcattccgc cttcagaaag gtcaggcggc ggacaatctg ttcggcggca
     3301 tcctgccgca tttccgccca caatgccgct tcctcttcct gtttgcccaa gatctcgttg
     3361 tcggcataag caaggacgcg gcggacggac acggtcatcg gtttgccgac cggctcgccg
     3421 cgtttcaata cctgcgcttc aaccgtcaat atcaaaagat attcgttgat gactgccgca
     3481 cgggtaacgg tgtaggtttc cttgttttgg gaaacgctgt ctatacgcag ggtcatctgc
     3541 gcgccggcag catcgtccac cctgcccgaa gcctgataca gcgcggtttc caaaggaaac
     3601 tgcaatgcct gtccgccttc gatgtgccag ctccggtagg tcagcggcgg agaaatgccg
     3661 tctgcacctt tcaggtggaa accgcacgcg cccagcatca gcactgcggc agtaagaaac
     3721 agtttgttca tggcttgcct ttctcaaaag cacaaagtcg gtagattata tcagcggcgt
     3781 gttcagacgg cattgccgtc cagccaaatc aggctcgcca tacgccctgt cgctccgtcg
     3841 cggcggtagg aaaagaaagt gtcccgttcc aaaaccgtac aatgcgtgcc gccatatacg
     3901 ccgcccacgc cttcacgctt cagaatcagg cgggcgagcg cgtaaaggtc ggcaaggaat
     3961 ttgccgccgc ctatgccttc aaatgcggcg gcggcttcgg gcatgggcgt gcagaacgca
     4021 tcaaacacat cctgtccgac ttcaaacgca tccgcactga tggcagggcc gagatacgcc
     4081 atcatttcga cgggcggaac cttcatcgcg gcaatggtgt tttgcaatac gccgcccgcc
     4141 aaaccgcgcc agcccgcgtg tgcggcggca accgtcgtac ccgccctgtc gcaaaacaaa
     4201 acaggcaggc agtctgcggt catcacggca caggcaacct tgcccgtgtc gtccacggaa
     4261 gcgtccgcat cgggcgtgcc gtccaatgct tcggcagcat tgacgacaat ggtgctgtgg
     4321 atttgattga ggtaggcaac gggcaatccg acctgctgtt gcacgatttc gcggttgcgg
     4381 agcacggctt cgggattgtc gccgacgtgc gtaccgaggt tcaaactctg atacgcacct
     4441 tggctcacgc cgccgttgcg cgtggtaatc agggttttca cattggcggg cgcgggccaa
     4501 tcggcggtca ggaagttttt gcctttcggg gcaagattta gggtttctgt gatggttttc
     4561 attggatatt ggaataggtg tgtggcggaa aaggttgatt aactgccttc ggggcaaatg
     4621 gatgcttgtt ttttcgtcat tcccgcacag gcgggaattc ggagattcgg gattgttttc
     4681 aaacgttcgg gtttggattc ttgccggtgc aggaatgatg cccttatata ctttctccga
     4741 gctttatatg tttcaacagg ggatggcaca tcaagcaccg cactgcgtgt tgcccgaacg
     4801 agactaccct ccgattagat tctatcgcta taaacagatg ggtttcaacc gaaaaggaat
     4861 ggggataaag tccatttccg acatctctcg ggcgatgccg tctgaaaacc aatctccact
     4921 ttcagacggc attgtttagg cgcggtcatt ttgcaaatta aggttaaggc gtgtatgctt
     4981 gccttccctt aaaaacaaac acgcaacgca ttattatgaa tatcctcagt aaaatcagca
     5041 gctttatcgg aaaaacattt tccctctggg ccgcgctctt tgccgccgcc gcttttttcg
     5101 cgcccgacac tttcaaatgg gcggggcctt atattccttg gctgttgggc attattatgt
     5161 tcggtatggg tttgacgctc aaaccttccg acttcgatat tttgttcaaa catcccaaag
     5221 ccgtcatcat cggcgtaatc gcacaattcg ccattatgcc ggcaaccgcc tggctgctgt
     5281 ccaaactgtt gaacctgcct gccgaaatcg cggtcggcgt gattttggtc ggctgctgcc
     5341 cgggcggtac ggcttccaat gtgatggcct atctggcgcg cggcaatgtg gctttgtcgg
     5401 ttgccgttac gtctgtttcc accctgattt ccccattgct gactcccgcc atcttcctga
     5461 tgcttgccgg cgaaatgctg gaaatccaag cggccggtat gttgatgtcc atcgtcaaaa
     5521 tggttttgct ccccattgtt ttgggtttga ttgtccataa ggttttgggc agtaaaaccg
     5581 aaaagctgac cgatgcgctg ccgctggttt ccgttgccgc catcgtgctg attatcggcg
     5641 cggttgttgg ggcaagcaaa ggcaagatta tggaaagcgg cctgctgatt tttgcggttg
     5701 tcgtactcca caacggcatc ggctacctgc tcggcttctt tgccgccaaa tggaccggcc
     5761 tgccttatga tgcacaaaaa acgctgacca tcgaagtcgg tatgcaaaac tcgggcctgg
     5821 ccgccgcgct tgccgccgca cactttgccg ccgcgccggt cgttgccgtt ccgggcgcat
     5881 tgttcagcgt gtggcacaat atctccggct cgctgctggc aacttattgg gcagccaaag
     5941 ccggtaaaca taaaaaaccc taaacgcctt tcatcataat gccgtctgaa cggggcaaac
     6001 aaaaccggcg gtttctgccc ggccccttca gacggccttt gtttgaaatc ggcggggacg
     6061 attgcccggc tgctccgatt cggctgcctg cgttcgcccc gcctttcaag ccgcctcact
     6121 cccttacata aaccacttcg acatcatagt cgtcttcgtt ccaatcatcg tcgtcgtccg
     6181 cgccgaattt gtcctgccat tcttcttcat tgctcaaaga cgaatccaaa ccggcttcaa
     6241 gacggaggac agacaacaaa tgatatatgt cgtccggaat cggtgcctca aacgaaacgg
     6301 tttcgccgct ttccggatgg gtaaaactca agcggtaggc gtgcaacgcc tgacgcgcac
     6361 ccaaactttt aacggcttct ttcaccgtgt cgccgcacgg atggcgcggg ttgccgtaaa
     6421 ccgggtcggc ggcaagcgga tggttggcct cgcgcatatg gacgcggatt tggtgcgtcc
     6481 tgcccgtttc gagcgagcat tcgatatagc tgtgggtaag atagcgttcc aacactttga
     6541 cgtgggtaac ggcaggcttg ccgccgaatt tgacgactgc cattttcagg cggttgtgcg
     6601 gatcgcgtcc gatttgggtt tcgattttac cgtcaaaggg gacgatgccg ttggcgacgg
     6661 cgcggtagat acgtttgacc gtgcgttctt gaagctgcct cacgagggaa ttttgcgccg
     6721 acagggtttt ggcaaccacc atcagcccgc tggtttcctt gtccaaacgg tgtacgatgc
     6781 ctgcacgagg tacttggctc aattcgggac agtgcgccaa gaggccgttg agcagcgtcc
     6841 ccgtccagtt gcccgccgcc ggatgcacca ccagtccggc cggtttgttg acgacgatga
     6901 cggtatcgtc ttcgtaaata atatccaaag ccataggctc tggaacaaac gccagatttt
     6961 cctcactcgg acggacggtt acacaaattt gctcgccgcc tatcattttg tctttgggtt
     7021 gcgaaggttt atcgtttaca ataaccgcgc cttctttaat ccatgatgtc aggcggctgc
     7081 gcgagtagtc gggcagaagt ttcgccaaca ccgcatccaa ccgcccgccg gcaagctcga
     7141 gcggaacggt cagaccgata cggttttcag tttcggaagc tgacgcaaag tctgaatcgt
     7201 cgctataatc ggattcatta tcaaaggaag tattctgcat gaaaaaaatt cttttaacgg
     7261 tttcattagg tttggcactg agtgcctgtg ccactcaagg tacggtcgat aaagatgctc
     7321 agattaccca agattggagt gtggagaagc tctatgccga agcccaggac gaattgaaca
     7381 gcagcaatta tacgcgggct gtcaagttat acgaaatctt ggaatcgcgc ttccccacca
     7441 gccgccatgc ccggcaatcc caactggata ccgcatacgc ctattataaa gacgatgaaa
     7501 aagacaaggc tctggcggca atcgaacgct tccgccgcct ccatccgcag catccgaata
     7561 tggattacgc gctgtatctg cgcggcttgg tgctgttcaa cgaagaccag tccttcttga
     7621 acaaactggc ctcgcaagac tggtccgacc gcgacccgaa agccaaccgc gaagcgtatc
     7681 aggcctttgc ggaactcgtc caacgcttcc cgaacagcaa atacgccgcc gatgcaaccg
     7741 cacgcatggt caaactggtc gatgcactgg gcggcaatga aatgtcggtg gcgcgctact
     7801 acatgaaacg cggtgcatac atcgccgccg ccaaccgcgc ccaaaaaatc atcggcagct
     7861 atcaaaatac acgctatgtc gaagaatcgc tcgccatatt ggaacttgcc taccaaaaac
     7921 tcggcaaacc acagcttgcc gccgatacgc gccgcgtgtt ggaaaccaac ttcccgaaaa
     7981 gcccgttttt gacgcacgct tggcagcccg acgatatgcc ctggtggcgt tactggcact
     8041 gattccgcac cgatgtcatg atgccgtctg aaaccctttc agacggcatt ttccgttccg
     8101 gcaccagcct ttccgttcac tcaaacggtt ttttctgcca atagaattta taccctctca
     8161 aacgttgggc cttcacgccg ccgcgcccca agccgaattg cagcgcgccc gacaaatcgg
     8221 tacgcagcag tttgatgccg tgtgcgcgga cacggttctg caccgcttcg gtcggatgtt
     8281 tgtaggcgtt ggcataaccg cttgaagcaa cggcatattc gggcgagacg gcattgagga
     8341 acacgcccga cgaggacgta ttgctgccgt gatgtcccaa aaccaaaacc tggctgtaca
     8401 ggttgcctcc atacttgccg accaggcttt cctcgccctt cgtatccaaa tcgcccgtta
     8461 ccagcagtgc cgcaccgccc gccacaacac gcaaaacaca acttttccca ttatcatcga
     8521 tgtttttgcg ttcagacggc atcaaaaact caaaatccac gccgtcccat tgccaacgct
     8581 gttccgcaca atgccgcgcc ccctcataaa attccggctg tccggcataa atcccgctgt
     8641 tcggtatctt gccgactgcc tgaaaaccgc cgtcgtggtc gctgtcgtga tgcgacagaa
     8701 ccagcttgtc caaacggcgg acacccgccg cattcaaact cggcacaatc cccatctgcg
     8761 ccgccgctgc cgttccggtg tcaaacaaaa gatgatgatt tgccgtctga accgacaccg
     8821 acaaaccctg ccccgcatcc caaaccgtaa ccgcaacctc attttccggc acgccgggtg
     8881 aacggtaaaa cacaaaccct gccaacagca acaccgccca cggacgcaaa cccaagccgc
     8941 gcggcagcag caacagcaaa gcggcacaca ccgccaacac caacagcggc aaaggtgcgg
     9001 aggcaacggc aaactcgggc gacacatcgg caagccacac caaaaaccgc aaagtatatt
     9061 ccgccaaaaa tgcccccaac tgttgcaaag gcgcaaacgg cacgaccgaa cccagcaacg
     9121 ccagcggcgt caatacccaa gaaaaccacg gaatcgccac cgcattgacc aaagggctga
     9181 ttaaaggcag cgaagcaaac agataaccga gcaaaaccag cgacaacacc gaagccgccc
     9241 actgcccgcg cacggcggtt tgccgtttcc cttcgtgcaa acgccccgaa cacgcccata
     9301 tcagggccgc caccaaaccg aaagacagcc aagtccccac acccaagacc gccaaagggt
     9361 cgaacagcag cactgccgcc aacgcctgcc accacgtcgc ccacgccgac aatcttcccc
     9421 tgcgccaagc ccacgcaaac gccgccaaca tcaaaacgct gcgctgcgtc ggcacggaaa
     9481 aaccggcaag cagcgcgtaa aacagcgcgc ctgcacaccc cgccgccaaa acccacacgc
     9541 gcggccgggc aggaaggcgc ggggaacagg caagcagccg cttcgccagc cacgcaaaca
     9601 tcaccgccac catcgtaacg tgcaaacccg aaatactgac caaatgcgtc agccccaacg
     9661 gtcggaacgc ctgccacaat tcggggcgca atgccgactg ttcgcccacg ctcaacgcgc
     9721 gcatcagccc gatgccgtct gaaagcccgc cgtccgcatc cgcctgctgc caattacggc
     9781 tgatgcggct gcgccaaacc gcaatccccc aaccgcttcc gccatgcagc aaaaccctgt
     9841 ccgcaccgac cgtccccgcg ccgcctatcc cgttggataa tgcccacgcc tcacggttca
     9901 aaccgcgcag gttcaattcg ccgacgacgg ggtgcacacg tgccgttatc cgccatctgc
     9961 tcccgaccgc ccattcgcgc cgtttgtagt ccgacagcag caaatcaaac gtccgaccac
    10021 cgctgtccac agcctttgcc gcaaactgca cgcgccgccc gtccgacctc ggcatatccg
    10081 ccacttccac cgtcaacggc acacctgaaa cggcttctgc ccaccattgg gaagacaatg
    10141 ccgcctccgt cctgaatacg ccgtaagccg cgcccgccaa cacgcacagc atcagaccgg
    10201 caaacgcaaa acgccttgca aacacagcaa aaaccgcaaa agccgccagc caaaacagcc
    10261 aatgcggcac gaccggcagc gcgaacgaag ccgccgcacc gacaacccaa cacggcaaaa
    10321 accaacgacg gaacacgtct ccccacccca gatatttcaa aaataaacgg ataataaagc
    10381 aaatccgaaa aaccgtcata acaagatttg aaaatgatat agtggtttaa ca
//
LOCUS       DAXHSJ010000056        10431 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000056 DAXHSJ010000000
VERSION     DAXHSJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.056"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(4..1005)
                     /locus_tag="ACFM8U_001048"
     CDS             complement(4..1005)
                     /locus_tag="ACFM8U_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC6334304.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPLARLKQWLGMMKKEFAAAQNLFDRVRTVKDADEVRNILAEFEREMNT"
     gene            complement(1082..4915)
                     /locus_tag="ACFM8U_001049"
     CDS             complement(1082..4915)
                     /locus_tag="ACFM8U_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC6334305.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVGRFDSSFDETSQKRRQILERLSQITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEFVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKTRWEWLLANLDTPLADYKARYGAA
                     VHAAPEAKNNESCFIAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLSNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQIGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKNVPAKTPRSLLGIEDGKSIPIIRNPASPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASNLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5176..5272
                     /gene="ffs"
                     /locus_tag="ACFM8U_001050"
     ncRNA           5176..5272
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFM8U_001050"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5439..7130
                     /gene="dld"
                     /locus_tag="ACFM8U_001051"
     CDS             5439..7130
                     /gene="dld"
                     /locus_tag="ACFM8U_001051"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC6334306.1"
                     /translation="MSASQLLSRLTQTVGEKYIITEPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINDNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7262..7369
                     /locus_tag="ACFM8U_001052"
                     /pseudo
     CDS             7262..7369
                     /locus_tag="ACFM8U_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7425..7796
                     /locus_tag="ACFM8U_001053"
     CDS             7425..7796
                     /locus_tag="ACFM8U_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC6334307.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            7951..8499
                     /locus_tag="ACFM8U_001054"
     CDS             7951..8499
                     /locus_tag="ACFM8U_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6334308.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLAQTEINPELQAYA"
     gene            8633..9724
                     /locus_tag="ACFM8U_001055"
     CDS             8633..9724
                     /locus_tag="ACFM8U_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6334309.1"
                     /translation="MNAQTLIANVAELVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGRVLAGTGMSNTVKHLAGIEKHNLQAERADGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNRYLDDGYDSLKARLD
                     GAWAETERLAGLAWNNTPDTLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10196..10366
                     /locus_tag="ACFM8U_001056"
     CDS             10196..10366
                     /locus_tag="ACFM8U_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC6334310.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2433 a         2912 c         2801 g         2285 t
ORIGIN      
        1 tattcaagta ttcatttctc gctcaaattc agccaagatg ttccgaactt cgtccgcatc
       61 cttaaccgtt cggacgcggt cgaacagatt ttgtgctgct gcaaattctt tcttcatcat
      121 acccagccac tgtttcagcc gcgccagcgg atatttgttg tttgcctctt ttgtcaggca
      181 cagctcgaaa aactgccgta tccatttgga aacttcggca aaatccgtgt ctttgaccgg
      241 cccgccgttc tcgtattgct tgatttgccg cgccaaatcg gggcggatga ccgcgccgcg
      301 accgagcatc acgctgttgc agccgctgat tgttttgatg ccgatatagt cttgcaggct
      361 gaaaacgtcg ccgttggcgg taacgggaat attgacgctg tctcggattt tccttatcca
      421 ttcccaatgc gccggcggtt cgtaaccctc ggctttggta cgcgcgtgta cggtcagtcc
      481 gcatgcgccc ccttcggcaa tcgcacaggc gcattccaaa gcccggcttt tgtcttcgta
      541 accgagccgc atttttgcgg tgagcggaat atgtgcgggc aaacgtccgc gcagcgtttt
      601 gacgatgtgg aatatcagtt ccggctcttt taaaaggatt gcgccgcctt tgtgtttgtt
      661 gacggtgggc gcggggcagc cgaagttcaa atcgattttg ttcgcaccga agcggacggc
      721 ttccagcgca ttcgccgcca tattgtccgc atcgctgccc aaaagttgga cggtgcaggg
      781 cgtgccggaa aacgttttgt ttccgttggc gatttcgggg acatatttta accatatgga
      841 tcgggaatgc acggtatggg taatgcgtac aaattcgctg acgcattcgt cgtagccgcc
      901 aatacgcgtc agcaggtcgc gcatcacgtc atcgaccaga ccctgcatag gggcaaggat
      961 gattcgggtt ttttgtttgg gttcggttgt ttgtccgtca atcatgattc gggtaggtat
     1021 ttgattcaaa caatgccgtc tgaaggcttt cggcgttcag acggcatttc cgtgtccgtt
     1081 gttacagcaa tactttttcc acgccgccgt tgttggcttt tttcacaaac tcgtccagcc
     1141 agttttcgcc gaggatgtgt ttcgccattt cgataacgat gtagtcggca ggcatattgt
     1201 tgtcgtcggc gtagcggctc aaaccttgca ggcaggcggg gcaggaggtc agcattttga
     1261 cgggttcacc ctgcggcagc tctttgaggt ttttctcgat ttcctcttgt ttgcggaatt
     1321 tgacttgggt ggcgatatcg gggcgtttga cggcaaacat accggattcg ccgcagcagc
     1381 ggtcgcttaa aacgactttc tgccccatca ggttgcttgc catttgggtg gcgttcatgg
     1441 ttttgatggg tgtgtggcag gggtcgtggt agaggtattg ctgacctttc acgccgtcga
     1501 gtttcacgcc tttttcgagc aggtattcgt ggatgtcgat gatgcggcag ccagggaaga
     1561 tttcctcgaa gcggtatttt tcgagctggt cataacaagt gccgcaactg acgacgacgg
     1621 ttttgatgtc gaggtagttg agggtgttcg ccatacggtg gaaagccacg cggttgttgg
     1681 tgctcatttc ttcggctttt gccttattgc cgcccgcgtc ttgcggatag ccgcaacaca
     1741 tatagccggg cggcagaacg gtttgtacgc cgacgtgcca gagcatagct tggacggcaa
     1801 gcccgatttg gctgaacagg cgttccgaac cgcagccggg gaagtagaac acggcttcgg
     1861 catcttccgg agaagccgga ttacggatga tggggatgct tttgccgtct tcgatgccca
     1921 ataaggagcg cggtgttttg gcgggtacgt ttttgggcag ggggcggttg atgaaatgga
     1981 taacctgttc tttaatcggg gctttgccga cggttgcctt gggttcggct ttttgctttt
     2041 tcgtgccgat tggaagaagt ttgccgattt tgtaggcgaa attctgcgct gggaagccga
     2101 tctgtatcat ggcggcgcga agggctttga tggttttcgg gccggtggcg ttcaaaaacg
     2161 ccatacccat agctgcggca ggcgcaaatc gtttgtggcc ggaatcggca agatagttgc
     2221 ggacggctac ggtaacgtcg ccgaagtcga tgttgacggg gcagggtttg acgcagcggt
     2281 ggcacacggt gcagtggtcg ccgatgtcca tgagttcttc aaagtgtttg atggaaacgc
     2341 cgcggcgggt ttgttcttcg tataagaagg cttcggtcaa taagcccacg ccgaggattt
     2401 tgttgcgcgg actgtacagc aggttggcac gcggaacgtg agtagagcag acgggtttgc
     2461 atttgccgca gcgcaggcag tctttgacgg aatcggcgat ggtaccgagg tctgattttt
     2521 ccataatcaa cgattccgca cctaaaagct cgaaggatgg cgtataggcg ttgcttaagt
     2581 ccgagccttt catcagtttg tgacggttga aggtgtgttt cggatcgact tggtttttgt
     2641 agtcccaaaa cggctgcatt tcttcgtcgc ttaaaaattc gagcttggta atgccgatgc
     2701 cgtgttcgcc ggaaatcacg ccgttaagcg aacgggcgat tttcataatg cgttcgactg
     2761 agcggtatgc cgtctgaagc atttcggcat cgtctgagtt aaccggaata ttggtgtgaa
     2821 cgttaccgtc gccggcgtgc atatgcaggg cgacaaagac gcggctgcgt acggtttttg
     2881 cgtggatttt tcccaagcct tggataattt tggtgtcggt tttgccgctg aagatttcag
     2941 aaagcggttt cattacgtcc gctttgacag acacgcgcag gcggaaatcg cggaaggcaa
     3001 taaagcagct ttcattgttt ttggcttcgg gtgcggcgtg gacggctgcg ccgtagcggg
     3061 ctttgtagtc ggcaagcggc gtgtcaagat tggcgagcag ccattcccaa cgcgttttga
     3121 cggcggaaac gtgggccagg gcgtgtttgc cgcgttcgcc caatagttcg gcggtcggca
     3181 ggtcggttcc cattttgtcg atggggagtt tgccggacag atattgctcc aaggcggcac
     3241 agagtttgag tttgttttga atggaaagct caatgttgat gcgttcgatg ccgtctgaat
     3301 actcaccgag cctttcgagt gggatgacca cgtcttcgtt gattttaaag gcgttggtat
     3361 gtttggcgat ggcggcggtg cggctgcggt cgagccagaa ggttttgcgc gcttcgggcg
     3421 atacggcgat aaagccttcg ccgtcgcggg cgcgggcaag ttcacagatg tgctcggcgg
     3481 cggcctctac ggcggcttcg tcgtctgaaa ccacgtccgc cagtaagacc attttcggcc
     3541 gccccttacc cgctgctttg gtggcgtagc cgacggcgcg gacgtaacgc cagtccaaat
     3601 gttccaaacc cgccagccgc acgctgtcgt gggcgagcag gaaatcgcgg atttcgacga
     3661 tagaaggcgt agcggtggca accgtgccga aaaactccat acacacggtg cgcgtgtatt
     3721 tcggcatttt gtgcaacaca aaggcgacgc cggtgatgat gccgtctgta ccttcttttt
     3781 gcacgccggg caggccgctc aagaatttgt cggtaacgtc tttgcccaaa ccgactttgc
     3841 ggaatttgtg gccggggatt tccaagcgtt cggttttaac gatattgatg ccgtctgaat
     3901 ccagcgtgtg aacatcgaac acggcggttt cttcgtcgtg gattttgccg aaattgtggc
     3961 gcacgcgctc gatacgcagc cattcgcctt gagggttgac catctgccag taggcgaggt
     4021 tgtccaacgc cgtcccccac agcacggctt ttttgccgcc cgcgttcatc gccacattac
     4081 cgcccacgca agacgcatcg gcggaagtcg gatcgacggc gaacaccaaa cctgcctgat
     4141 gcgcggtttc ttccacccgc cgcgtaacca cgcccgcgcc gcaccggata atcggatgtt
     4201 tgccatccaa gcccgccagt tcgacaaact ccacgccgcg atgtttgtcc agcttttccg
     4261 tattgatgac cgcgctcatt gcgtccaaag gaatcgcgcc gccggtataa cccgtaccgc
     4321 cgccgcgcgg gataataacc aaatccagct cgattaaggc gcgcaccaaa ggcgcgatct
     4381 ctgcctcggt atcaggattg acgacgacaa acggatactc cacgcgccag tcggttgcat
     4441 cggtaacgtg cgttacgcga gccaagccgt caaacataat attgtgcggc ttggtgattt
     4501 ggctcaaacg ctccaaaatc tgccgccgct tttggctggt ttcatcaaaa ctgctatcaa
     4561 aacgcccgac cgccttttcc gccgccgcaa ccaagacatc gacttggcgg ttgtcgtcgc
     4621 ggcgtttgcg gatttcgttc aacctgtggc gcatttcgcg taccagcgcg gcgcggcgtt
     4681 tcggatgctc cagcaaatca tcgaccagat acggattgcg tacgaccacc caaatatcgc
     4741 ccaatacttc aaacagcatc cgtgccgaac gcccggtttt acgctgtccg cgcaggtctt
     4801 gcaggatttg ccacgcttcg tcgcccaata atcggatgac gatttcgcgg tcggtataag
     4861 aagtatagtt gtagggaatt tcccgaatac gctgaggggc ggtagtcgtg gtcatggtgt
     4921 gtccgtgttt ggcagttttt atttgaatgc cgtctgaaaa ttcggtttca ggcgagtttg
     4981 aaaaggcgat aatgtagttc aatttttgat atttttcaat gccgaaacag gaaataccct
     5041 tatagaaaca aaataacgct ttgagaaatt gttgacaata ttatcaaata taaattttag
     5101 gtggtgtttc cggcgtatga ccgtatcaaa acggttgagt ttttcagaaa acccgttata
     5161 atgccgtttc tctgaggcgg gtctccccgc atggcaaatc ggaacaccgg gtcaggggcg
     5221 gaagccagca gcccactccg atgcgccagt gccgggggtt tggcccgccg ccctatttga
     5281 aacgccggag ctttgatgtt ccggcgttcc cgtatcgcac cgatgccgtc tgaaagccgt
     5341 tcagacggca ttttctttac tttgccataa cgtataacta caatgtgaaa acaattccaa
     5401 tgtgcaatgc acctgccgac aattttaagg aaactgccat gagcgcatca caattactgt
     5461 caagactgac ccaaaccgta ggcgaaaaat acatcatcac cgaacccgcg aaaaccgaac
     5521 aataccgcca aggctaccgc ttcggcgagg gtaaggcgtt ggcggtggtg cgccccggaa
     5581 gcattctgga aatgtggaaa attctacagg cgtgcgtcga agcggacgtg attgtgatta
     5641 cgcaggcggc gaataccggt ttgacgggcg gctcgacccc cgacggcaac gattacgacc
     5701 gcgacatcgt gatagtgaac actatgcgga tgaacatcat ccaaaccatc aacgacaacg
     5761 aacaagtcgt ctgcctgccc ggctcgaccc tgaaccagct tgaattgctg ctgaaacctt
     5821 tgggacgcga accgcattcg gtcatcggct catcctgcat cggcgcgtcc gttttaggcg
     5881 gcgtgtgcaa caactccggc ggcgcgttgg tacagcgcgg cccggcctac accgaaatgg
     5941 cactgttcgc ccaaatcaac gaagaaggca ggttggaact ggtcaaccac ttgggcatag
     6001 acttgggcga cacgcccgaa gaaatcctga ccaacctgca aggtcatcat tatcaaaaca
     6061 aagacatcaa acaagacgcg ggcaaagggc acgaccacgc ctattgcgaa cacgtccgcc
     6121 aagtggacga accgaccgcc gcgcgtttca atgccgaccc cgcccgccat tacgaagcct
     6181 cgggctgcgc gggcaaactg atggtttttg ccgtccgttt ggacaccttc ccgcaagaaa
     6241 aacaaaccgc cgtgttctac atcggcacga acgacatcaa cgagctgacc gacatccgcc
     6301 gcgccgcctt gggcgaattt gaaagcctgc ctgtttccgg cgaatacatc caccgccacg
     6361 ctttcgacat tgccgacgtg tacggcaaag acacgtttta cgtcatcaaa aaattcggta
     6421 cgcaccaact gccgaaatta tttgacttga aggcgcgcgt ggacaggttc ggcaaaaaag
     6481 ccagcttcct gcccaaacat ttttccgaca aggccatgca gttcgtcagc aaatacctgc
     6541 ccgaccacct gcccaaatcc atgcgcgatt accgcgacaa atacgaacac cacctgattc
     6601 tgaaaatggg cgggaaaggc gtggatgagg cgcgcgcgtt cttaaaagaa tattttgcac
     6661 accacagcgg cgcgtttttc gagtgcaacg ccgaagaaac ccaagccgcg atgctgcacc
     6721 gtttcgccgt cgcctccgcc gccatccgct accgcgccgt gcatgatgac gaagtggaag
     6781 acttggtcgc gctcgacatc gccctgcgcc gcgacgatcg cgactggttt gaaaaactgc
     6841 cgtcggaaat cgacaataaa atcatccata aactctacta tggacatttc atgtgccacg
     6901 ttttccatca ggattacatc atcaaaaaag gcaacgactg catggcgttg gaacacgaaa
     6961 tgctgcatct tttagaccaa cgcggcgcgc aatatcccgc cgaacacaac gtcggtcatt
     7021 tgtatgaagc caaacccgcg ctcaaacagt tttaccgcaa acttgacccg accaacagct
     7081 tcaacccggg cgtaggcaaa accagcaaaa agaaaaactg ggcggaataa gcgcgtccgc
     7141 tttgaaggca ggcaatgccg tctgaaggca aaacgccgtt cagacggcat tgtcgggttt
     7201 gaagttcccc gtcgggtaat cgctttcaaa ccgtatgccg acacacgggc gcgcccctcc
     7261 caaatatagt ggattaaatt taaatcagga caaggcaacg aagccgcaga cagtacagat
     7321 agtacggcaa ggcgaggtaa cgccgtactg gtttaaattt aatccactat aaagtctgat
     7381 cctaccttcc caaagggcag ggtttcaacc gaaaaggaaa cacgatgaaa ccatacaaaa
     7441 tctacaccca tcccgccctg ccgccccaag ccgtcaaaca aggctggtca tggccggggc
     7501 tcttgttcgg cacgctgtgg gcgtgtttca aaagaatgtg gggtttgggg ctggggctga
     7561 cgggcgcgat attcgtgctg gctgtgtttg cccaactggt ttacggcgac acgcccgcca
     7621 cagactcggc gtttaacgta ttgggcttgg cggtttccgt ctggttcggc gcaaagggca
     7681 acagccttta tgcacgccac cttttatcgc gcggctatac cgaattgccc gaaacggttg
     7741 aggcagccaa tccccaagcc gcattggcgc aatatttcgg gcgcgggggc cgataagcgc
     7801 gttttctttt ccatcatgcc gtctgaaaaa tgttcagacg gcatttctta taaccttttg
     7861 tttgcagcac ataaccaatc agtctttcct attccgttcc gaaatgcata ccatgcgccc
     7921 gtacccaata tttgcgaaca aggaaagaaa atggcacgtt taaccgtaca caccctcgaa
     7981 accgcccccg aagccgccaa agcgcgcgtc gaggcggtac ttcaaaacaa cggctttatc
     8041 cccaacctta tcggcgtatt atcaaacgcc cccgaagcct tggcgtttta ccaagaagtc
     8101 ggcaagctca acgccgccaa cagcctgacc gccggcgaag tcgaagtcat ccagatcatc
     8161 gccgcccgta ccaaccaatg cggtttttgc gtggcagggc acaccaaact cgcaaccctg
     8221 aaaaaactcc tgtccgaaca atccgtcaaa gccgcgcgcg ctttggcagc aggcgaattt
     8281 gacgatgcca aactcggcgc gctcgccgcc ttcacccaag ccgtaatggc gaaaaaaggc
     8341 gcggtatccg atgacgaact caaagcattt ttcgacgcgg gctacaacca gcagcaggca
     8401 gttgaagtcg tgatgggcgt agccttggca accctgtgca actacgtcaa caacctcgcc
     8461 caaaccgaaa tcaaccccga attgcaggct tacgcctgat acggcaaacc gcccgaatat
     8521 cgggcggttt ttcaaccccc ctcctcaaac aaaaacaagc cgccaagcgg cagggaaagc
     8581 agccgcgcat caggcgcgcc cccgattccc ccgcaaacct taaggaacaa agatgaacgc
     8641 ccaaaccctg attgccaacg ttgccgaact cgtcaaaacc aagctcaaac ccatagtgga
     8701 cgacatcgac cgcaaaggat actaccccga agcatttatg cgcgaactcg gcgcaatcgg
     8761 cggattcggc gcagtcggga cagaagccga aggcggcaac ggcttaggct tggcaacgca
     8821 aatcgccgtt ttgcgcgaaa tcggcaaaga atgcggcgca acctccttca gcgcgtggtg
     8881 tcaggcggct tgcgcgtggt atctgcacca aacgcccaac caagccgtca aagacaaata
     8941 cctcgccgac atcctgcaag gcagagtatt ggcaggtacc ggaatgtcca ataccgtcaa
     9001 acaccttgcc ggcatcgaaa aacacaacct tcaagccgag cgcgcagacg gcggctacac
     9061 agtcaacggc gcgctgccgt gggtgtccaa catcggcgaa gaccacatct gggcaaatac
     9121 cgcccaaatc ggcggcggct acgtcatgtt tatcaccggc ggacaatggg aaggcgtaag
     9181 cctgcaaaac tgccccgaat tttgcgccct cgaaggtacg cgcaccttca gcctgaactt
     9241 caaagacgta tttatccccg acgaagacat catcgccgcg cccgaacagt ttgccgacta
     9301 catccaaagc atcaaagcag gtttcatcct cctgcaaatc ggcatcggcg cgggcgtgat
     9361 tgacggcagc ctcggcatca tccgcctcgc caacatcgtc aacgccgaag tcaaccgcta
     9421 tctcgacgac ggctacgaca gcctcaaagc aaggttggac ggcgcatggg cagaaaccga
     9481 acggctcgcc ggtttggcat ggaacaacac gccggacaca ttagcgaccc tcaagctgcg
     9541 cgaagccgcc gccgtactgg cacttgccgc cgcccaatcc gccgccctgc attccggcgc
     9601 aaaaggctac ctgatgcgca gtcccgccca aaggcgcgtc cgcgaagcaa tgtttgtcgc
     9661 catcgtaacc cccgcgatca aacacctccg aaaagaaata gcggcaatcg aagccgcaaa
     9721 ataaacaggc ggaaaaaacc gaaaccccca ccgcgtcatt cccgcgaaag cgggaatcca
     9781 gcccccaacg caacaggaat ccatcggaaa aaacagaaac ccccaaaccg tcattcccgc
     9841 aaaagcggga atccagcccc ctaacgcaac aggaatccat cgaaaaaacc gaaacctcca
     9901 aaccgtcatt cccacggaag tgggaatcca gtaaccgaaa aaccacagga atctatcgaa
     9961 aaaaacccca ccccccaaaa aaaccgggcg gatacctcac catcctccca aaccctgatt
    10021 tatagcggat gaacaaaaat cagggcaagg cggcgaaacc gcagacagta caaatagtac
    10081 gaaaccgatt cacttggtgc ttgagcacct tagagaatcg ttctctttga gctaaggcga
    10141 ggcaacgccg taccggtttt tgttcatccg ctatacaaaa aacaaggaaa cacaaatggc
    10201 gcaatatatg tgcggcccct gcggctggat ttacgatgaa gaactcggcg acccagaaca
    10261 cggcatcccc cctgggacaa agtttgaaga cattcccgac gactggaaat gccccgaatg
    10321 cggcgtaggt aaagaagatt tctacctgtt ggatttcgtg atatagcggc agccgcaccc
    10381 ttagggaaaa ccccgtcatc cggcgttccg cctgcggcgg cagggaacgc c
//
LOCUS       DAXHSJ010000057        10374 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000057 DAXHSJ010000000
VERSION     DAXHSJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.057"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..489
                     /locus_tag="ACFM8U_001057"
     CDS             <1..489
                     /locus_tag="ACFM8U_001057"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFC6334311.1"
                     /translation="LPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQI
                     RDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGV
                     ARYEDLITFVQDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQWYLDNKTWWQ
                     NA"
     gene            594..1460
                     /gene="rfbA"
                     /locus_tag="ACFM8U_001058"
     CDS             594..1460
                     /gene="rfbA"
                     /locus_tag="ACFM8U_001058"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC6334312.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            1514..2053
                     /gene="rfbC"
                     /locus_tag="ACFM8U_001059"
     CDS             1514..2053
                     /gene="rfbC"
                     /locus_tag="ACFM8U_001059"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6334313.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAIDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIGWPLQTA
                     PLLSPKDLAGKVLAEAVTF"
     gene            2077..4191
                     /locus_tag="ACFM8U_001060"
     CDS             2077..4191
                     /locus_tag="ACFM8U_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFC6334314.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLSTLLPEF
                     HIYKDGKGAEAVVGWGLRPTTHKARAFATEHQLPFIALEDGFLRSLGLGVAGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQTMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQNVDKVYCVTSQMGFEALLCGKPLTT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMGI
                     YFNAEAPSRLEHILQNQNFDDQDFQTALKLQKMLTENHISKYNVGNSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYHNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQADILTCLQYADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGCFAKKLGKIKQLYRSFK"
     gene            4328..5587
                     /locus_tag="ACFM8U_001061"
     CDS             4328..5587
                     /locus_tag="ACFM8U_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFC6334315.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            5954..7360
                     /locus_tag="ACFM8U_001062"
     CDS             5954..7360
                     /locus_tag="ACFM8U_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6334316.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     DHLLQNGHPEQALPWLEAAAAQRHPKALFTLLQQREHNGTPTGQLLNEYAWLSEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVATAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAISNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            7578..8792
                     /gene="gltS"
                     /locus_tag="ACFM8U_001063"
     CDS             7578..8792
                     /gene="gltS"
                     /locus_tag="ACFM8U_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC6334317.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            8891..9907
                     /locus_tag="ACFM8U_001064"
     CDS             8891..9907
                     /locus_tag="ACFM8U_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6334318.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AASDAGVPQAAEDAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            9918..10115
                     /locus_tag="ACFM8U_001065"
     CDS             9918..10115
                     /locus_tag="ACFM8U_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334319.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            10133..10282
                     /locus_tag="ACFM8U_001066"
     CDS             10133..10282
                     /locus_tag="ACFM8U_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334320.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAVFSCVSYADVSTHKTAHKAL
                     PYVPS"
BASE COUNT         2658 a         3000 c         2441 g         2275 t
ORIGIN      
        1 ttgccgacca ttgtaaccaa ctgctccaac aactacggtc cttaccattt tccggaaaaa
       61 ctcattcctt tgatgattct gaacgcgctt gacggcaaac cgctgcctgt gtacggcgac
      121 ggtatgcaaa tccgcgactg gctgtttgtc gaagaccacg cgcgcgcact gtatcaggtt
      181 gttaccgaag gtgttgtcgg cgaaacctac aatatcggcg gccacaatga aaaagccaat
      241 attgaagtcg tcaaaaccat ctgcgccctg ctggaagaac tcgctcccga aaaaccggcc
      301 ggtgtggcgc gttatgaaga tttgattact ttcgtacaag accgccccgg ccatgacgta
      361 cgctacgccg tcgacgcagc caaaatcagg cgggatttgg gctggctgcc tttggaaacc
      421 ttcgagtccg gcctccgcaa aaccgtgcaa tggtatctgg acaacaaaac ctggtggcaa
      481 aacgcataaa acggcaactg ccgtccggaa cgcttaggta cgggcaaata gttttcagac
      541 ggcatcccga cgcaatgccg tctgaaaacc catcgcaaag gaagaaaaca aagatgaaag
      601 gcatcatact ggcaggcggc agcggcacgc gcctctaccc catcacgcgc ggcgtatcca
      661 aacagctcct gcccgtgtac gacaaaccga tgatttatta ccccttgtcg gttttgatgc
      721 tggcgggaat ccgcgatatt ttggtgatta ccgcgcctga agacaacgcc tctttcaaac
      781 gcctgcttgg cgacggcagc gatttcggca tttccatcag ttatgccgtg caacccagtc
      841 cggacggctt ggcacaggca tttatcatcg gcgaagaatt tatcggcaac gacaatgttt
      901 gcttggtttt gggcgacaat attttttacg gtcagtcgtt tacgcaaaca ttgaaacagg
      961 cggcagcgca aacgcacggc gcaaccgtgt ttgcttatca ggtcaaaaac cccgaacgtt
     1021 tcggcgtggt tgaatttaac gaaaacttcc gcgccgtttc catcgaagaa aaaccgcaac
     1081 ggcccaaatc cgattgggcg gtaaccggct tgtatttcta cgacaaccgc gccgtcgagt
     1141 tcgccaaaca gctcaaaccg tccgcacgcg gcgaattgga aatttccgac ctcaaccgga
     1201 tgtatttgga agacggctcg ctctccgttc aaatattggg acgcggtttc gcgtggctgg
     1261 acaccggcac gcacgaaagc ctgcacgagg cggcttcgtt cgtccaaacc gtgcaaaaca
     1321 tccaaaacct gcacatcgcc tgcctggaag aaatcgcttg gcgcaacggt tggctttccg
     1381 atgaaaaact ggaagaattg gcgcgcccga tggcaaaaaa ccaatacggc caatatttgc
     1441 tgcgcctgtt gaaaaaataa tgtttgaggc cgtctgaaac ttttcagacg gcctttagat
     1501 gaaagataaa aagatgaaca tcattgatac cgccattcct gacgtaaaac tgcttgagcc
     1561 ccaagtcttc ggcgacgcgc gcggcttttt tatggaaacc ttccgcgacg agtggtttaa
     1621 aacccaagtc tgcgaacgca ccttcgtgca ggaaaaccac tccaaatccg gcaaaggcgt
     1681 attgcgcggc ctgcactacc aaaccgaaaa cacacaaggc aaactcgtac gcgtagttgt
     1741 cggcgaagtg ttcgacgtgg ccatcgatat gcgcgaaggt tcgccgactt tcggcaaatg
     1801 ggcgggcgca accctgtccg cgcaaaacca atgccagctt tggatacccg aaggcttcgc
     1861 gcacgggttt tgcgttttgg gcgacgcagc ggaagtcgtg tacaaatgca cggactatta
     1921 caagcctgaa gccgaacagg ttttaatatg gaacgacccg acagtcggca taggctggcc
     1981 gcttcaaacc gcgccgctgc tgtcgcccaa agatcttgcc ggcaaagtat tggctgaagc
     2041 ggtcacgttt taagactatt caggccgtct gaaagaatgt tcctcttttc agacggccta
     2101 caatctatta ataacaataa tcgacgaaaa cgcattgtga aaaacgccta catcccctct
     2161 cgcggcatcc gcaaaatccc ccatctctcc accctattgc ccgagtttca tatctacaaa
     2221 gacggaaaag gagcagaggc tgttgtcggc tggggtttgc gcccgacgac acacaaagcg
     2281 cgtgcttttg ccaccgaaca ccagcttccc tttattgctt tggaagacgg ctttttacgc
     2341 tcgcttggcc tgggcgttgc cggctatcct ccctattcca tcgtctatga cgacatcggc
     2401 atctactacg acactacccg cccttctcgt ttggaacaac tgattcttgc cgccgatacc
     2461 atgccgtctg aaaccttggc tcaggcgcaa cagacgatgg atttcatcct gcaacaccac
     2521 ctgtccaaat acaaccacgc gcccgaactt tcagacgatc atcctttacg ctccccatcc
     2581 aagcctgaaa ccgtcctcat tatcgaccaa accttcggcg atatggccat ccaatatggc
     2641 ggcgcagacg cctctacgtt tgaactgatg tttcagacgg ccttgaatga aaacccgcaa
     2701 gccgatatct gggtaaaaac ccatcccgat gttttgtgcg gcaaaaaaca aggctatctg
     2761 acccaactgg cgcagcaaca ccgcgtccat cttctggcag aagacatcaa cccgatttct
     2821 ttgttgcaaa acgtcgataa agtttattgc gttacctcgc aaatgggttt tgaggcactt
     2881 ttgtgcggca agccgctgac caccttcggc ctgccgtggt atgccggatg gggtgtaagc
     2941 gacgaccgcc atcctgaaat caaccgcctt gttcaaaccc aacgccgcgc cacccgcaac
     3001 ttgctgcagc tcttcgccgc agcctatctg caatacagcc gctacctcaa ccccaatacc
     3061 ggcgaagcag gcagcctctt tgatgtcatc gactatctgg cgacggtcaa acgtaaaaac
     3121 gacaaattgc gtggcgagtt atattgcgtc ggtatgtctt tgtggaaacg cgcggttgcc
     3181 aaaccgttct ttaacgtacc ctcttgccgt ctgaaattta tctcttccac ccaaaaactg
     3241 gcaagggtca aactgtccga cgatgcacgc atcctggctt ggggcaacgg caaagaggcc
     3301 atcgtccgct ttgccgaaca acaccacatc cccctgctgc gcatggaaga cggctttatc
     3361 cgctcggtcg gactcggctc caacttagtg ccgccgctgt cgctcgttac cgacgatatg
     3421 ggtatttact tcaatgccga agccccatcc cgccttgaac acatcctgca aaaccaaaac
     3481 ttcgacgatc aagactttca gacggccttg aagttgcaaa aaatgctgac cgaaaaccat
     3541 atcagcaaat acaacgtcgg caactcagac tttaccgccc catcaaccga caaaaccgtg
     3601 atcctcgttc ccggtcaggt tgaggatgat gcgtctatcc gctatggttc gccccaaatc
     3661 tatcacaatc tggatttgct ccgtaccgta cgcgaacgaa accccaatgc ctatatcatc
     3721 tacaaaccgc atcccgatgt agtcagcggt aaccgcatcg gccatatttc ccctgaagat
     3781 gctgcacgat atgccgacca aaccgccgaa caagccgaca tcctgacctg tctccaatac
     3841 gcagacgaaa tacataccat gacttcgctg accggttttg aagccttgtt gcgcggcaaa
     3901 aaagtcagct gctacggcct gcctttttac gcaggctggg ggcttaccca agatctgctc
     3961 cccatcccgc gccgtagccg cagacttgag ctttggcagc tgattgccgg cacgctcatc
     4021 cactatcccg actacatcca ccccgaaacc catcaggcca taaatgcgga aaccgcagcc
     4081 caaatcctga tacgacaaaa aaatatgcaa aaaaacaaca acggattaca tcgcgggtgc
     4141 tttgccaaaa aattaggtaa aatcaaacaa ctatatcgat ctttcaaata aataccatca
     4201 aagttaacga tgcgtcataa acttgcctct attgcggcat cattgccttt gcatcgttaa
     4261 ttctcttggc gtatgcttga aagttcaacc taaaactatt acataaaaaa caaaaccaca
     4321 ttgcaacatg aaacagaccg tcctcaaaaa taacctgcaa aacctgcttg aaagcgcaga
     4381 aaatatcctg ctgcttcaag gccctgtcgg cgattttttt ctgcgccttg ccgactggct
     4441 gactgcaaac ggcaaaaccg tacataaatt caactttaat gcaggcgacg actattttta
     4501 tccgcccact caagcgcata ccgttgtttt taacgacaac tacgatgcct ttcctgagtt
     4561 tttgcaagaa tacatcactc aacatcacat ccaggccgtt gtctgctttg gcgacacacg
     4621 cccttatcac gtcattgcaa aacgcattgc aaacgaaaac caagccagtt tctgggcgtt
     4681 tgaagaaggc tatttccgcc cctactacat caccttagaa aaagacggcg tcaacgcatt
     4741 ttccccgttg ccgcgccgtg ccgacttttt tcttgaacaa ttccctaagc ttgcccagca
     4801 agaatataaa gcgccaacgc cggtacacgg cggttttacg cccatggcaa aaaacgctat
     4861 ccgttactat atcgagttgt tccgcaatcc acgcaaatac cccgactaca tccaccaccg
     4921 cgcacccaat gccggccatt acctcaaacc gtggtcgctc tccatcctca agcgtttgaa
     4981 ctactatatt gaagacatcc aaatcgccaa acgtgtggaa gcaggcaaat acggcaagtt
     5041 ttttattgtt cccttacagg tattcaacga cagccaagtc cgtatccatt gcgactttcc
     5101 cagcgtccgc agcttcctgc tccatgtttt gagttcattt gccgagcacg cgcctgccga
     5161 taccaacatc atcatcaagc atcatccgat ggaccgcggt tttatcgact actggcgcga
     5221 cattaaacgc tttatcaaag aacaccccga actcaaaggc cgcgtgattt atgtccatga
     5281 tgtccccctg cccgttttcc tgcgccacgg tctcggcatg gtcaccatca acagcaccag
     5341 cggcctgtcc ggactgattc acaatatgcc agttaaggtt ctcggccgtg cctattatga
     5401 tattcccggc attactgacc aaaatacctt ggcagaattt tggaatcatc cgacaccgcc
     5461 tgacaaagag ctgttccatg cctaccgaat gtaccacctc aacgtgaccc aaattaacgg
     5521 caacttctac agtcaggtgt ttttccccaa caaaaaaacc tccaactctt ccacaccagt
     5581 aatctgactt agcgaaggaa gttcaggccg tctgaaaaca tttcagacgg cctgaaacaa
     5641 tcaatacctt agctactgcc atgtaaataa aacacaaaaa tctgcattta tcattaacaa
     5701 taaattacaa aaacagtata atgaccgagc tgccatgagc gcataccgac tcaacctgag
     5761 ccctttataa cacacaaaat atggatgtat ccccaggcaa aacaatataa caagccaaac
     5821 atcctaaaga taagccggca aggaaataca ctctgcaaaa cgatgccgag caatattttt
     5881 acaaagccct caaccggtat cgccgcccat atgccgcagc atccgtcttc cactttatat
     5941 ccgcccgcaa accatgaccg ccgctcctga tatcctatac cgtcaagccg ccgccctttt
     6001 ggaacaatcc aataccgccc aagccctgcc cctgctgcaa caggccgcag agcagggata
     6061 tacggaagct gcttttgtat tgggcgacca tctgctgcaa aacggtcatc cggagcaggc
     6121 acttccatgg ttggaagccg ccgcggccca acgccatccc aaggcactct ttaccctgct
     6181 gcaacaacgc gaacacaacg gcaccccgac cggacagctt cttaacgaat acgcctggct
     6241 gagtgagcaa ggtcacctcg aagcccaatt aattctcatg cgttaccaca cgcagcgcaa
     6301 cgatccacaa tcgctctact gggcggagct tgccgctgcc caatatgccg cacctgcgta
     6361 ttaccatttg gcacgccatc atcaacgcca aggcgacgtt gcaacagcta tcgaacaata
     6421 cgaaaaagcg gcagcgctcg gcgtgactgc agcctgctgg caactcggtc aaatctactt
     6481 ctacggcaca ggtgtcagcc ccaaccacgc acaagccgaa cactatctcg aacaagccgc
     6541 gcaagccggc cacatcgccg cacaaaccct gctggccgac cttcttgctg cccaacgcaa
     6601 acccgaagcc ttagaatggt atcgtcgtgc cgccgacaaa gagcaagcgg aagcacaggc
     6661 caagctggct caatacgccc tgaccggcga actttccgaa cgcgatccgt ttcaagcggc
     6721 acgatatgcc aaagccgccg ctgagaaaga ccatcccgaa gccctgaaaa tcatgggcga
     6781 cctctaccgc tacggtctcg gtatcaaagc cgacaaccat atcgcgcaag attactacca
     6841 ccgtgccgcc gcgctgggtt ctgccgccgc agcacaaaaa ctcatcagcg acgccgcgct
     6901 gtaccatccg caacaatacg aacaaatcaa aactgccgcc ctgcaacaac aacaaaccga
     6961 aaccatctac cgtttggcgg aagcacaagc ctgcgccatc ggccgtcccg ccgactacaa
     7021 tgccgcgcga aaaaattaca tggaagctgc cgggttccac cataaaaacg cagcggcagc
     7081 cttaggccgc atctaccatt acggcctcgg tacggcgcaa gatcctcggg cggctgcaca
     7141 ctggtacgcc atcgctgccg aacaaaacca cccttccgcc caataccacc tcgcctgttt
     7201 ttactatcac ggacaaggtg tcggctgtca tgttccgacc gcctgctact ggctgcaggc
     7261 tgccatcagc aacggccaca cttcggccga atcattaata tccctattag aacaatggcg
     7321 acgcgaagca caccatgcca tcggacaaaa ggccgtctga aaagatttac actcgcattt
     7381 tttgacaatc tttaactatt cccctaatat ttgccagtta tttttcacgg acacgccatt
     7441 gttttcattt ctttctgaaa acaccttgtc cgcgcatcaa taccatgaca ctcggcggat
     7501 aacgccaagc gttgaaacac actacatccg gaacaaaaac ggatgctcgg aaaaatattt
     7561 ctaggaggtg aaacaacatg gaatgggaat tcaacagtta ttacacactg attgccgcca
     7621 cgctcgtgtt gctggttggt aaatttctgg ttcaaaaaat caaattctta cgagacttca
     7681 atattcccga gccggtcgct ggcggtttgg ttgccgcgat tatcctgttt gcgctgcacg
     7741 aggcgtacgg cgtgagcttc aaatttgaga aaccgctgca aaatgcgttt atgctgattt
     7801 ttttcacgtc catcggcttg agcgcggatt tttcccgttt gaaggcgggc ggtttgccgc
     7861 tggtggtttt taccgcgatt gtgggcggat ttatcttggt gcaaaacttt gtcggggtcg
     7921 gactggctac ggctttgggt ttggacccgc tcatcggtct gattaccggt tcggtgtcgc
     7981 tgacgggcgg acacggtacg tcaggtgcgt ggggacctaa ttttgaaacg caatacggct
     8041 tggtcggcgc aaccggtttg ggtattgcat cggctacttt cgggctggtg ttcggcggcc
     8101 tgatcggtgg gccggttgcg cgccgcctga tcaacaaaat gggccgcaaa ccggttgaaa
     8161 acaaaaaaca ggatcaggac gacaacgcgg acgacgtgtt cgagcaggca aaacgcaccc
     8221 gcctgattac ggcggaatct gccgttgaaa cgcttgccat gtttgccgcg tgtttggcgt
     8281 ttgccgagat tatggacggc ttcgacaaag aatatctgtt cgacctgccc aaattcgtgt
     8341 ggtgtctgtt tggcggcgtg gtcatccgca acatcctcac tgccgcattc aaggtcaata
     8401 tgttcgaccg cgccatcgat gtgttcggca atgcttcgct ttcgcttttc ttggcaatgg
     8461 cgttgctgaa tttgaaactg tgggagctga ccggtttggc ggggcctgta accgtgattc
     8521 ttgccgtaca aaccgtggtg atggttttgt acgcgacttt tgttacctat gtctttatgg
     8581 ggcgcgacta tgatgcggca gtattggctg ccggccattg cggtttcggc ttgggtgcaa
     8641 cgccgacggc ggtggcaaat atgcagtccg tcacgcatac tttcggcgcg tcgcataagg
     8701 cgtttttgat tgtgcctatg gtcggcgcgt tcttcgtcga tttgattaat gccgcgattc
     8761 tcaccggttt tgtgaatttc tttaaaggct gattttccgc ctttccgaca aagcacctgc
     8821 aaggtttacc gcctgcaggt gcttttgcta tgatagccgc tatcggtctg caccgtttgg
     8881 aaggaacatc atgtatcgga aactcattgc gctgccgttt gccctgctgc ttgccgcttg
     8941 cggcagggaa gaaccgccca aggcattgga atgcgccaac cccgccgtgt tgcaaggcat
     9001 acgcggcaat attcaggaaa cgctcacgca ggaagcgcgt tctttcgcgc gcgaagacgg
     9061 caggcagttt gtcgatgccg acaaaattat cgccgccgcc tacggtttgg cgttttcttt
     9121 ggaacacgct tcggaaacgc aggaaggcgg gcgcacgttc tgtatcgccg atttgaacat
     9181 taccgtgccg tctgaaacgc ttgccgatgc caaggcaaac agccccctgc tgtacgggga
     9241 aaccgctttg tcggatattg tgcggcagaa gacgggcggc aatgtcgagt ttaaagacgg
     9301 cgtattgacg gcagccgtcc gcttcctgcc cgtcaaagac ggtcagacgg catttgtcga
     9361 caacacggtc ggtatggcgg cgcaaacgct gtctgtcgcg ttgctgcctt acggcgtgaa
     9421 gagcatcgtg atgatagacg gcaaggcggt aaaaaaagaa gacgcggtca ggattttgag
     9481 cggaaaagcc cgtgaagaag aaccgtccaa acccacgccc gaagacattt tggaacataa
     9541 tgccgccgcc agcgatgcgg gcgtacccca agccgcagaa gacgcgcccg aaccggaaat
     9601 cctgcatccc gacgacggcg agcgtgccga taccgttacc gtatcacggg gcgaagtgga
     9661 agaggcgcgc gtacaaaacc agcgtgcgga atccgaaatt accaaacttt ggggaggact
     9721 cgataccgac gtgcaaaaag agttggtcgg cgaacaacgc aagtgggcgc aggaaaaaat
     9781 cagcaactgc cgacaagccg ccgcgcaggc agaccggcag gaatacgccg aatacctcaa
     9841 gctgcaatgc gacacgcgga tgacgcgcga acggatacag tatcttcgcg gctattccat
     9901 cgattagggg caaaccgatg aataccgtcc caaaaagcag gattcccgtc aaaccgctgc
     9961 ccgaaaaaac cacagacgaa gccaaagtcg aaaaatggcg gcagctcggt gcggaacacg
    10021 gtttgtcggg cgaatgggca gttgccgcca gattgggcga aaacggtttt accgaagaac
    10081 agatggaaaa tatcgccaac ctgttcggca gataaagaga aaattgacgg aaatgccgtc
    10141 tgaaaccctg ttgtcggttt cagacggcat tttgaccaat acggtacgca ggcgcaaaac
    10201 agccgtcttt tcctgcgttt cctatgctga tgtttcaaca cacaagacgg cacataaagc
    10261 gttgccctat gtgccgtcct gattcggaag gggttacgcc cctcccaaat atagtggatt
    10321 aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag agaacgattc ttta
//
LOCUS       DAXHSJ010000058        10355 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000058 DAXHSJ010000000
VERSION     DAXHSJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.058"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            50..277
                     /locus_tag="ACFM8U_001067"
     CDS             50..277
                     /locus_tag="ACFM8U_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334321.1"
                     /translation="MNVWDIWSRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVL"
     gene            complement(275..390)
                     /locus_tag="ACFM8U_001068"
                     /pseudo
     CDS             complement(275..390)
                     /locus_tag="ACFM8U_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            607..1908
                     /gene="bioA"
                     /locus_tag="ACFM8U_001069"
     CDS             607..1908
                     /gene="bioA"
                     /locus_tag="ACFM8U_001069"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC6334322.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVEAQIKQMSHVMFGGLTHDPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDEADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            1905..2552
                     /gene="bioD"
                     /locus_tag="ACFM8U_001070"
     CDS             1905..2552
                     /gene="bioD"
                     /locus_tag="ACFM8U_001070"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC6334323.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEDLLTIDYIQQQDYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLIFNHIHDSSDECVAQDSLNYLQCRLKDSFPEAEWLELDKTDAV"
     gene            2567..3043
                     /locus_tag="ACFM8U_001071"
     CDS             2567..3043
                     /locus_tag="ACFM8U_001071"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC6334324.1"
                     /translation="MEHLFGKWLPDLPADVSDGISLPMVRLLHTRSLTAALRPLPHTF
                     SVELLKLGELETECGGRLVREVLLKLDRIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVAGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            3073..3963
                     /gene="ubiA"
                     /locus_tag="ACFM8U_001072"
     CDS             3073..3963
                     /gene="ubiA"
                     /locus_tag="ACFM8U_001072"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC6334325.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFTANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            4148..4597
                     /gene="ptsN"
                     /locus_tag="ACFM8U_001073"
     CDS             4148..4597
                     /gene="ptsN"
                     /locus_tag="ACFM8U_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC6334326.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            4601..5563
                     /gene="hprK"
                     /locus_tag="ACFM8U_001074"
     CDS             4601..5563
                     /gene="hprK"
                     /locus_tag="ACFM8U_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC6334327.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            5544..6398
                     /gene="rapZ"
                     /locus_tag="ACFM8U_001075"
     CDS             5544..6398
                     /gene="rapZ"
                     /locus_tag="ACFM8U_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC6334328.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIAYLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            6480..7421
                     /gene="ylqF"
                     /locus_tag="ACFM8U_001076"
     CDS             6480..7421
                     /gene="ylqF"
                     /locus_tag="ACFM8U_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC6334329.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIGMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            7522..8124
                     /locus_tag="ACFM8U_001077"
     CDS             7522..8124
                     /locus_tag="ACFM8U_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC6334330.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVIQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPLTDEEEPEAVPSDTLPN"
     gene            8308..9981
                     /gene="recN"
                     /locus_tag="ACFM8U_001078"
     CDS             8308..9981
                     /gene="recN"
                     /locus_tag="ACFM8U_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC6334331.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKSVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGWSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEECLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            10091..10167
                     /locus_tag="ACFM8U_001079"
     tRNA            10091..10167
                     /locus_tag="ACFM8U_001079"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10125..10127,aa:Arg,seq:acg)
     gene            10185..10259
                     /locus_tag="ACFM8U_001080"
     tRNA            10185..10259
                     /locus_tag="ACFM8U_001080"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10218..10220,aa:Glu,seq:ttc)
BASE COUNT         2496 a         2604 c         2814 g         2441 t
ORIGIN      
        1 atagggctgt aaccttcaat cggaatttgt tgcctgcggg atatacggta tgaatgtttg
       61 ggatatatgg agcaggatgg tggaaatcta tcataagtat aagaagccgt gcctggtttt
      121 ggcggtggat tttgtgatgg gtatggtatt catagagccg aatgaggagc cgtgcatcgg
      181 taggtgctat gcgcctatgt cggagtcccc tgattttgct aacgctgttg cgatggctgt
      241 tgctatgatc tgtatcgtat ggattgccgt tttatagtgg attaaattta aaccagtacg
      301 gcgttacctc gccttgccgt actatttgta ctgtctgcgg cttcgtctcc ttgtcctgat
      361 ttttgttaat ccactatatc tatgactgat tgaagcgttg ggcggaggct gcgtgaaacg
      421 gtattgggcg ttgggccgtc tgattccaat cgggcttgag gaatgcgaaa cggtgtgcgc
      481 ttatgttgcg gaacgatttg tttcgcggtt ttgcgcccga aacggatgga aaggtgtggg
      541 aagcggtctg tcggagtaga atacgcgttt gcgtttgaat acagtaagaa gaaaagagag
      601 aaacttatgc cgtctgaaca tcaacacaca tcatcattgc ttgatttcga ccgtacccat
      661 ctgcttcatc cctatacttc catgaccgat ccgcttcccg tttatcctgt caaacgtgca
      721 gaaggggtgt ttatcgaatt ggcggacggc acacggctga ttgacgggat gtcgtcttgg
      781 tggtgtgcga tacatggtta caatcatcct gttttgaatc aggcggttga ggcgcagatt
      841 aagcaaatgt cgcatgtaat gtttggcggt ttgacgcacg atcctgcggt ggaactgggc
      901 aagttgttgg tcggaatttt gccgcagggg ttggaccgta ttttttatgc ggattctggt
      961 tcggtttcgg tagaagtcgc gttgaaaatg gcggtgcaat accagcaggc gcgggggttg
     1021 acggcgaagc agaatattgc gacggtacgg cgcgggtatc acggcgatac ttggaacgcg
     1081 atgtccgtct gcgatccgga aacggggatg caccatattt tcggcagcgc gttgccgcag
     1141 cgttattttg tcgataatcc gaaaagccgt ttcgacgatg aatgggacga ggcggatttg
     1201 cagcctgtcc gcgccttatt tgaagtgcat catgcggata ttgccgcctt tattttagag
     1261 ccggtcgtgc agggcgcggg cggcatgtat ttttatcatc cgcagtatct tcgcggattg
     1321 cgcgatttgt gcgacgaatt tgatatcgtt ttgatttttg atgaaatcgc caccggattc
     1381 gggcgcacgg gcaagatgtt tgcctgcgaa cacgcggagg tcgtgccgga tattatgtgc
     1441 atcggcaagg gtttgagcgg cggctatatg acgctggcgg cagcaatcac ttcgcaaaaa
     1501 gttaccgaaa cgatttcgcg cggcgaagcg ggcgtgttta tgcacggccc gacgtttatg
     1561 gcaaacccgc tggcgtgtgc cgttgcctgc gcttcggtca aactgctttt gtctcaagac
     1621 tggcaggcaa atatccgccg cattgaaagc atcttaaaag gccgtctgaa agccgcgtgg
     1681 gacattcgcg gcgtgaaaga cgtgcgcgtt ttaggtgcca tcggggtgat cgagctggaa
     1741 aaaggcgtgg atatggcgcg ttttcaagcg gattgcgtgg cgcagggcat ttgggtgcgc
     1801 ccgttcggca ggctggtgta tctgatgccg ccttacatca tttcagacgg cgttttgacc
     1861 aaacttgccg acaaaaccgt gcaaatcttg aaggaacaca gcaaatgaaa ggcgtttact
     1921 tcgtcagcgg catagacacg gacatcggca aaaccgtcgc caccggcgtg ttggcaaaac
     1981 aattgttgca gcagggcaaa agcgtgatta cgcaaaagcc cgtgcaaacc ggttgccaaa
     2041 acattgccga cgacatcgcc gtccatcgta aaatcatggg taaacccatg caggaagccg
     2101 acaaacaagg gctgaccatg cctgaaatct tcagttatcc agcttcgcct catctcgctg
     2161 cccgtcttga cggcagggct ttggacttgg acaaaatccg caccgccaca caagaattgg
     2221 cggcgcagta cgaagtcgtt ttggtcgaag gcgcgggcgg attgatggtt ccgctgacgg
     2281 aagatttgtt aaccattgat tatattcagc aacaagatta tcccgtcatc ctcgttacca
     2341 gcggacggct gggcagtatc aaccacactt tactcagttt cgccgcgctc aaacaatacg
     2401 gcatccgcct gcacagcctg attttcaacc atattcacga cagttccgac gaatgtgtcg
     2461 cgcaagacag cctgaactat ctgcaatgcc gtctgaaaga cagtttcccc gaggcggaat
     2521 ggttggagtt ggataaaaca gacgcggtat aaagattggg aaaaatatgg aacacctatt
     2581 tgggaaatgg ctgcccgacc tgcccgccga cgtttcagac ggcatcagcc tgccgatggt
     2641 gcggctgctg cacacccggt cgctgaccgc cgcattgcgc cccttgccgc atacattttc
     2701 ggtggaactg ctgaaactgg gcgaattgga gacggaatgc ggagggaggc tggtgcgcga
     2761 agttttgttg aagctggacc gtatccctgt tgtcgcggca aggagcgagt gccggatagg
     2821 ttcggcgttt tggcaaaaca ttttggactg cggcacgcgc cctttgggcg agcgtctgtt
     2881 tcaagccgat ttggaagggg cgcgttcggc gtttgagttt gccgttgccg gcgaaggatg
     2941 cggacggtac tttgccgcgc ggcgttctcg gttttcccgt cacggcgagg aaatgctgct
     3001 gaccgagtat tttctgcccg aactgaaacg ctttatcgga taaaataccg ttttttcaag
     3061 ctgtgcggca atatgaatcc taaatcccct ttatttttac gactgtccga ccgtttggat
     3121 gtgtacctgc gcctgatgcg ggcggacaag cccattggga cgctgctttt actgtggccg
     3181 acctactggg cattgtggct ggcttcagac ggcattcccg atttggcggt attggcggcg
     3241 tttacaatcg gtacgttttt aatgcgcagt gccggttgcg tcatcaacga ttttgccgac
     3301 cgcgattttg acggggctgt cgagcgcact aaaaaccgtc cgttcgcaca gggtcgggtc
     3361 ggaaaaaaag aagcgctgct gctgacggca tttttgtgcc tgcttgccgc attgtgcctg
     3421 attccgctga atcatctgac atggctgatg agcctgcccg cgctgtttct tgcgctgact
     3481 tacccgttta ccaaacgctt ttttccgatt ccccaactct atctcgggct tgccttttcc
     3541 ttcggtatcc cgatggcgtt tgccgcagtg ggcaacagcg tccccgttga agcatggata
     3601 ctttttaccg ccaatgtttt atggacactg gcttacgaca cggtttatgc aatggcggac
     3661 aaagaggacg atttgaaaat cggcatcaaa acctccgccg tcacgttcgg gcgttacgac
     3721 atcgccgccg ttatgctgtg tcacggaggc tttaccctgc tgatggcagt attgggggcg
     3781 gttatcggtg cggcatgggc atattggacg gcaatcccca tcgtcctgct gctgcaatac
     3841 cgccaatatg ccgccatcaa aagccgcgtc cggcaaatct gttttgaaac gtttttggca
     3901 aacaacagaa ttggttgggt gtggtttacc gccatttttg cccatacgtt tttcgcgaaa
     3961 taaggcaggg caatgccgtc tgaagagccg taaactgctt tggacggcat ttctatctgt
     4021 gccgaaaagc gttaaaatat gtttttaaaa cgctgtgtta tgtcagcccg taccgtatgc
     4081 gggattgaga tttgccccgg cagtcggtac aatctttctg ttttgcgatg tctgaaaaga
     4141 gaagcttatg agccttatcg gcgaaatttt gcctttgtcc catattgttt tggatatgga
     4201 ggtgggcagt aaaaaaaggc tgtttgagga agcaggcctg cttttggaac gcgaatcctc
     4261 attgtcccat gctgatgttt tcgaatgtct ttttgcccgt gaaaaactcg gttcgaccgg
     4321 tttggggcag ggcgttgcca tcccgcacgg ccgtcatgcc ggcgtgaagc aggcgacggg
     4381 cgcgttcatc cgcacgcgcg aacccgtcgg atttgacgca ccggacggca aaccggtttc
     4441 cctggttttt atcttgctgg ttcctgagaa tgcaacgggc gagcatttgg aggtattgtc
     4501 caaactggcc ggcaagtttt cccaaaaaag catccgcgaa tcgctgatga cggtttcttc
     4561 tgcggaagaa gcccgcgcca tcctgaccga agaataaaat atgcccagta tctctgtccg
     4621 ccgactgttt gacgacaacc aatacaagtt gcaactcgcc tgggccgccg gcaattcggg
     4681 tgcggacaac cgtatcggcg tagaggcgga caagcccgtc ctcgccttgg tcggacacct
     4741 gaatttcatc caccccaacc aaatccaagt ggtcggtttg gcggagtcgg aatatctgaa
     4801 ccgtctcgaa tcgggggaaa cgggttatca gttcggcgac ctgttcgaca tttctatgtc
     4861 tttggttatt gtggcaaacg gcttgccggt ttccccggga ctgcgcgact actgccataa
     4921 aaacgatatt ccactgctga cttccaaact cgaaagcccc tatctgatgg acgtgttgcg
     4981 gatttacctg caacgcaccc tggcggcatc gtccgtcaaa cacggcgtat ttctcgatgt
     5041 gtttgaaatc ggcgtgctga ttaccggaca ttcgggtttg ggtaagagcg aattggcgtt
     5101 ggagctgatt tcgcgcggcc acagcctgat tgccgacgat gcggtcgagc tgttccgcat
     5161 cggcccggaa acgctggaag ggcggtgttc gcctatgctg cgcgattttt tggaagtgcg
     5221 cggcttgggg atactcaata tccgccatat tttcggcgaa acttccatcc gccccaaaaa
     5281 aatcctgcaa ctcattatca atttggtcga ggcagacgac gagtatatga agcagctcga
     5341 ccggttgagt atccgcaccg aaaccgaatc catcctcaac gtcaacgtcc gttcggtaac
     5401 gctgcctgtc gcggtcggac gcaaccttgc cgtattggtt gaagcggcgg tacgcaatta
     5461 cattttgcag ttgcgcggta aggacagtac gcgcgaattt ttggaacgcc atcagacgca
     5521 acttaaagaa aacgaacaga atcatgaaaa tcgtcctgat tagcgggttg tccggttcgg
     5581 gcaaatccgt cgcactgcgc caaatggaag attcgggtta tttctgcgtg gacaatttgc
     5641 ctttggaaat gttgcccgcg ctggtgtcgt atcatatcga gcgtgcggac gaaaccgaat
     5701 tggcggtcag cgtcgatgtg cgttccggca ttgacatcgg acaggcgcgg gaacagattg
     5761 cctatctgcg cggactgggg cacagggttg aagttttgtt tgtcgaggcg gaagaaagcg
     5821 tgttggtccg ccggttttcc gagaccaggc gaggacatcc tctgagcaat caggatatga
     5881 ccctgttgga aagcttaaag aaagaacggg aatggctgtt tccgcttaag gaaatcgcct
     5941 actgtatcga cacttccaag atgaatgccc aacagctccg ccatgcagtc cggcagtggc
     6001 tgaaggtcga acgtaccggg ctgctggtaa ttttggagtc cttcgggttc aaatacggtg
     6061 tgccgaacaa cgcggatttt atgttcgata tgcgcagtct gcccaacccg tattacgatc
     6121 ccgagttgag gccttacacc ggtatggaca agcccgtttg ggattatttg gacggacagc
     6181 cgcttgtgca ggaaatggtt gacgacatcg aaaggtttgt tacgcattgg ttaccgcgtt
     6241 tggaggatga aagcaggagc tacgttaccg tcgccatcgg ttgcacggga ggacagcacc
     6301 gttcggtcta tattgtcgaa aaactcgccc gaaggttgaa agggcgttat gaattgctga
     6361 tacggcacag acaggcgcaa aacctgccga gccgttaatc ccattaaaca attatgccgt
     6421 ctgaaacccc gcttttctgt ttcagacggc atttcaaata ttgagaagaa aggaaagata
     6481 tggcaatcca atggtttccc ggtcatatga acaaggcgaa aaaagccatc gccgagcgtg
     6541 caaaaagcgt tgatatggtg attggaatgc tggacgcgcg tatgcccgcc tccagcgaaa
     6601 accccctgct tgcccagctt tccaaaggca aacccaaact taaaatccta aacaaacaag
     6661 atcttgccga ccccgaacgc acaaaggtct ggcttgacca ctacaacagc cgccccgaca
     6721 cgtgcgccat tgccctcgat tcctccgaaa ccggcgcaca cggcaaaatc acccaagcct
     6781 gccgcgcgat gattccccac cggcaaggca tagacaaacc cttgcgcgtc ttgatttgcg
     6841 gcattcccaa cgtcggcaaa tccaccctca tcaacggcat gataggcaaa aaatccgcca
     6901 aaaccggcaa cgaacccggc atcaccaaag ccgaacaacg ccttttcctt gccgacgact
     6961 tctggctgta cgacaccccc ggaatgctgt ggccgaaaat cattgtcgaa gaaagcggct
     7021 acaacctcgc cgccggcggt tcagtcggac gcaacgcatt ggacgaagaa gaagtcgccc
     7081 tcgaactttt agactacctc cgccgccact acctccctat gttgcaagaa cgctaccaag
     7141 ccgacaaaga ccccagcagc cactgggacg aaaacgtttg gctcgaatgg atagccaaaa
     7201 aacgcggcgc agtcctcagc ggcggacgga tcaactacca aaaagccgcc gaaaacatcc
     7261 ttaccgactt ccgtgaaggc aaaatcggca gaatcaccct cgaaacgccg aaccaatggg
     7321 aaacttggct caaaaaagcc cgtcagaaag aagccgaact caaagccata cgcgaagcca
     7381 gaaaagcaga gcgtaaaggg cagaagcctt cggaagcata aagaatgccg tctgaaaaat
     7441 atttttcagg cagcttctct ctactccaac cgatttcaga cggcatatcc aaacccatgc
     7501 cgtttcagca cggatacccg tatgaccgac aaaatttctc ccgacgcact aattgaagcc
     7561 gcactgctga cccaaaccga accgctgacc gaaaaatcta tgcgcgaatt gtgtgtgccg
     7621 ccactgtcgc aggacaaact gattgatgtg ttggcgcagt tgaaaacgcg ttggcaggat
     7681 agggcgttgc aactggtgca tacgcaagag ggctggcgtt ttcagattgt tcagacggca
     7741 ttcgagcggc tgggcagcct gcaagaacag cgtgcgccgc gctactcccg cgccgtgatg
     7801 gaaacactgg cgattatcgc ctaccagcag cccgtaacgc gcggcgacat cgaaggcata
     7861 cgcggtgtgg cggtgtcgca gaacgtgatc cagaccttgc aggatcgggg gtggattgaa
     7921 gtcatcggac atcgggacac attgggaaaa cccgcattgt gggcaacaac ggcaacgttt
     7981 ctcagcgatt tgcgcttgga cggtctggaa gaacttcctc cgctgaccga actgggcgaa
     8041 ctggttttgc ccgatttgat agaaatgccg cttacggatg aagaagagcc ggaagccgta
     8101 ccgtccgata ccctgcccaa ctgaaattcc aaatgccgtc tgaaacgcac attgcttcag
     8161 acggcattgc aacaaataag cagataaaaa caagtactaa gcaaaattaa ggaaaactta
     8221 tttttaattt aaaaaaatct tagttataat tcgtatatct aaagttgata ttgcttttgt
     8281 cggtagaatt gctaaggaat cctcacaatg cttctaacac tttctttgcg tgattttgtc
     8341 attgttgaaa atctgaatct ggattttcaa agcggcttta ccgtattgac cggagaaacc
     8401 ggcgcgggca agtccattac tttggatgcg attggtctgc tgttgggcga caaagccgat
     8461 tacagccaag tccgcagcgg tgcaaaagaa gcgcagttgt cggcattgtt cgatatttcc
     8521 catttgcctg ttttaaaagc agaattgtat gaacaggggc ttttaaacga cggagaagaa
     8581 gaactcagta tccgccgcat tatcgatgcc aaaggcaaaa gccgcagctt tatcaacaat
     8641 caggccgcta ccttggcgca actcaaatcc gtcggtagcc agcttatcga catccacggg
     8701 caaaacgccc atcattcgct taatcaggaa gccgcccagc gcgaattgtt ggacgcattt
     8761 gcgggtagca gggcgcaggc ggaaaccgtc aggcagcttt atcaaaattg ggccaatgcg
     8821 aaaaaagccc tccaagaggc gcaggaacac gccgatgccg tcattatcga gcgggagcgt
     8881 ctggaatggc agtttaacga actgaatcag ttggacatta aacaaggcga atgggaagcc
     8941 ctcagccaaa gccacgacag ccttgcccat tctgccgagc tgttgcaggc tgccgaagaa
     9001 gtcggaagca agattgacgg cgacaacggc atccaacgcc atatctatca atgtcaaaag
     9061 ctattggcca atctgcaaaa catcgagccg cgctttgccg agagcctgaa tatgttggca
     9121 agcatcgaag ccgaattggg cgaaatcagt gccaatatgc gcgatgtggc aggttggagc
     9181 gacatcaatc ccaatgaatt ggcggcacag gaacagcgta tgggcgaact gatggggatg
     9241 gcgcggaaat accggatcga acctgaagag ttgcctgcca agttggcaga aatcgaagag
     9301 tgcctgcaaa gcctgcaagc tgccgccgat ttggacgcgc tcgagcataa tgttgcccac
     9361 aattttgccg aatatcagga agctgcccac atcctttctg ccatgcgcca tcaggcggca
     9421 gagcgtttga gcggcgaaac gaccgagcat atgcaacacc ttgccatgaa aggtgcacgt
     9481 ttcgacatcg tcctgttgcc ttcgtcgccg acggcacacg gtttagagca ggttcaattt
     9541 caagttgccg ccaacaaagg caatccgccc cgtccgctga ataaagttgc ctccggcggc
     9601 gaattggcgc gtatcagcct tgccttacag gttgttgcca gtcaatatac ccaagttccc
     9661 accctgattt ttgatgaggt cgataccggt attggagggg gagtggctga aatggtcggc
     9721 aaggcattac gtgcgttggg cagaaaacat caggtgcttg ccgttaccca ccttccccaa
     9781 gtcgcatcct gcggagaaaa ccactggcgg gtgcgcaagc acagcgaggg agagcaaacc
     9841 gtcagcgaaa tcagtatatt ggatgaaatc caacggatcg aagaggttgc ccgtatgttg
     9901 ggcggagaag tcattaccga tacgacgcgg caacatgcgg cagaattgct gcaacttgcg
     9961 tcgaaaaata gtttatttta aaatcaatca gttaaaaaat aactaaaaat aaaagtctaa
    10021 aacaatagac agaactcaga taaatccgta ttatcacgct ttcttaatca cttgaacaag
    10081 tgattgtgct gcacccgtag ctcagttgga tagagtatct ggctacgaac cagagggtcg
    10141 ggcgttcgaa tcgctccggg tgcgccagta agaaaataca atatgcgccc atcgtctagc
    10201 ggttaggaca tcgccctttc acggcggtaa ccggggttcg attccccgtg ggcgtgccag
    10261 tttaaaatgc ctccgatatg atctgaggca tttcccattt cccatttccc atttcccatt
    10321 tcccatttcc catttcccat ttcccatttc ccatt
//
LOCUS       DAXHSJ010000059        10145 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000059 DAXHSJ010000000
VERSION     DAXHSJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.059"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(238..1455)
                     /locus_tag="ACFM8U_001081"
     CDS             complement(238..1455)
                     /locus_tag="ACFM8U_001081"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC6334332.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1657..2385)
                     /gene="rph"
                     /locus_tag="ACFM8U_001082"
     CDS             complement(1657..2385)
                     /gene="rph"
                     /locus_tag="ACFM8U_001082"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC6334333.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2467..2931)
                     /locus_tag="ACFM8U_001083"
     CDS             complement(2467..2931)
                     /locus_tag="ACFM8U_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC6334334.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3148..3924
                     /locus_tag="ACFM8U_001084"
     CDS             3148..3924
                     /locus_tag="ACFM8U_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6334335.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPGCQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4169..5023
                     /locus_tag="ACFM8U_001085"
     CDS             4169..5023
                     /locus_tag="ACFM8U_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC6334336.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5064..6272
                     /gene="pncB"
                     /locus_tag="ACFM8U_001086"
     CDS             5064..6272
                     /gene="pncB"
                     /locus_tag="ACFM8U_001086"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC6334337.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDDPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6364..8082
                     /gene="argS"
                     /locus_tag="ACFM8U_001087"
     CDS             6364..8082
                     /gene="argS"
                     /locus_tag="ACFM8U_001087"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC6334338.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIHAALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVSEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKTGEWDADAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8628..10046
                     /locus_tag="ACFM8U_001088"
     CDS             8628..10046
                     /locus_tag="ACFM8U_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6334339.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFVLTAPTPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGPMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHSKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2321 a         2997 c         2556 g         2271 t
ORIGIN      
        1 cttagctcaa agagaacgat tctctaaggt gctgaagcac caagtgaatc tgttccgtac
       61 tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataaatt
      121 taatccacta tattttttgt tccaaagtca aaatatgccg tccgaacatt cgggcggcag
      181 acaaaacggc actgcccgat aaaggcagtg ccgttgtccg tttcaaaccg tgaaacatca
      241 gcccaaatta aaggctttat gcaataccct ggttgccagt tccatgtatt tttcatcaat
      301 caatacggaa actttgattt cggaggtgga aatcatttgg atgttgatac cctcttcggc
      361 gagcgtgcgg aagattttgg cggctacacc gacgtgcgaa cgcataccca aaccgactgc
      421 agagactttg catacggtgt cgtcgccatc aatagaagcc gcgccgatac tgtcttggcg
      481 ttccgacagg atttccaaag tctgcttgta atcgccgcgc ggtacggtaa aggaaaaatc
      541 ggttgtgcct tcgctgccga cattttggat aatcatatcg acttcgatgt tggcatcggc
      601 gaccgcgccc aaaatctgat aggcgacacc gggtttgtcg ggtacgccgc gcacgttgat
      661 gcgggcttgg tttttgtcga atgcgatacc ggttacggca gctctttcca tgttgtcgtc
      721 ctcttcaaag gtaattaagg tgccattgcc gccgtcttgc aggctgctca gtacgcgcag
      781 gcgcactttg tactttccgg cgaattctac tgaacggatt tgcaagactt tcgagccgag
      841 gcttgccaat tcgatcattt cttcaaatgt aaccgtatcc atgcggcgcg cttcgggtac
      901 gacgcgcgga tcggtggtgt acacgccgtc cacgtcggta tagatttggc attcgtccgc
      961 tttgagggct gcggcaagcg caacggcgga agtgtcggaa ccgccgcgtc cgagcgtgga
     1021 aatatcgcct tcactgctga tgccttggaa gccggcaacg atgacgactt tgccggcggt
     1081 aaggtcggca cgcatttttt cgtcatcaat gctttcgatg cgggctttgg tgtgggcggt
     1141 atcggttttg agggcgacct gccagcctgt gtagcttttg gcatccacgc cgatgtcttt
     1201 caatgccatc gccaaaaggc cgatggttac ttgttcgccg gtggacaaaa ccacgtccaa
     1261 ttcgcgcgga tcgggatgct cctgcatttc gtgcgccaac gcgaccaggc ggttggtttc
     1321 accgctcatg gcggatacga cgacgacaat gtcgtgtcct tcggcgcggg ctttggcgac
     1381 acgtttggcc acgtttttaa tgcgttcggg cgagcctacg gatgtgccgc cgtatttgtg
     1441 tacgattaac gccatgtttc gtgctttctt gtgtgggatt ggtcgggcag catggtttgc
     1501 tggaaaagcg gctattatta ctatttttta catgaagttc aagaacggac tgcgctttcc
     1561 cgcctgccgt ttgacagcgg tcagcgaaaa acctgttctt tcagattgtt gacaaaatgc
     1621 cgtctgaacg gttttcagac ggcatccgga cgacaatcag gcggcggaca acgcattttg
     1681 ctggtgttgc agcagttcgc ctatgccttt ttgcgccagt gcaaccagtt tgcccaattc
     1741 gtccaaactg aacggcgcgt cttccgccgt accttggatt tcgatgattt ttcccgatgc
     1801 ggtcatgacg atattcacat cactgtcgca accggagtct tcgggataat ccaaatccaa
     1861 aagcggcacg ccgttcacta cgcctactga cacagcggca acggcttcgc ggatggggtt
     1921 ttcactcaaa atgccgtctg aaaccagttt gccgacggcg atttgcagcg cgacaaacgc
     1981 accggtaatc gaagccgtgc gcgtaccgcc atctgcctga atcacatcgc agtcaatcaa
     2041 gatttgtcgt tcgccgagtt tttccatatc tacgaccgcg cgtagcgaac gcccgataag
     2101 gcgttggatt tcttgtgtgc gcccggactg tttgcccgcc gaagcttcgc ggcgcatccg
     2161 ggaagcagtt gaggcaggca gcatcccgta ttctgcggtt acccagcctt ggtttttgcc
     2221 gcgcaaaaac ggtgggacat tttcgtctat ggaagcggta caaatcactt tggtattacc
     2281 gcattcaata aggcacgaac cgtccgtatg cggcaggaaa tgaggggtga ttttgatatc
     2341 gcgcaggctg tcggcggcgc gtgaggtgcg gatgtaatca ggcatactgc cctcccgtta
     2401 aaaacagata aattaaaaag ccttaaatat gaaaaatcac atttaaggcc ttcaaactga
     2461 aaatttctac gcctcttcgg ctttgctgcg gataatcaaa agcggcaggt ggctttggcg
     2521 cattaccgtt tcggcaaaac tgcccattaa aaggtgcatc agcccggtac gtccgtgcgt
     2581 acccaacacc agcaagtcgg caccgttttc atcggcataa tcaaccaaat cctgcgccat
     2641 ttcacgcgca cccttattgg caaccagcag gtgtttgacg gtattttcca cacccagttc
     2701 ccgggcggtg cgctcggcgg catccaaaac ttcgttgcct tgcgcgacgg cggcggcttc
     2761 gtagctttcg tgttgcaaaa attcgggggc gagcgccata tattcggcag gattggcaac
     2821 gtgtaccaaa gtcagacgcg caccgttgac cccggcaagc tcggcggcat gtttcagggc
     2881 attgatggac gtttcactgc cgtcaacggc aacaaccaga tgtttgtaca tatcgtattc
     2941 tccttttgca ccgcctcgcg gtgccctctt gtcggatggg cgcagggaca gtttgcgccg
     3001 tttcattata gacccgctgt cgggctttat acaacagccg aacagcccga ccgctttcca
     3061 gtataatatg ccgcttccgt gcagtcaggc attttttgcc ggctttcgtt cactttttga
     3121 tttgacgcaa tcttgcagga ttcgaccatg tccgacaacg ctttgacctc ttcgcgacgc
     3181 ttcggcggca tcgccagact ctacggagac tctgccttgg cgcacttttc acaggcacac
     3241 gtctgcgtag tcggcgtggg cggtgtcggc tcgtgggcgg tcgaggcttt ggcgcggacg
     3301 ggcatcggac gtttgacttt gattgatttg gacaacgttg ccgaatcgaa tgtcaaccgc
     3361 cagctgcacg ccctgaccgg cgacttcggc aaagcaaaag ttaccgcctt gcgcgaacgc
     3421 gtcgcccaaa tcaacccagg atgccaagtg tttgaaattg aagatttcgt taccgaagac
     3481 aatttgccgg aatacttcgg aaaaggtttt gatttcgtca tcgacgcgat cgaccaagtg
     3541 cgcgtcaaag cagcaatggc ggcttatttt gtggaacgca aacaaccgtt tgtcctcagc
     3601 ggcggcgcgg gcggacaaaa aaatccggcg ttaatccaaa ccgccgattt gagccgcgta
     3661 acccacgacc cgctgcttgc caacctgcgc tacaccttgc ggaaacgcta cggattcagc
     3721 cgcgatacga aagcaaatat gcgcgtgcct tgcgtgtatt cgaccgaaaa tatcgtgccg
     3781 ccgcagtcta gggaggcttg ttcggcagat gccgctccgc aaggcttgtc gtgcgccggc
     3841 tacggtgcaa gcatgctcgt taccgcttcg ttcgggctat attgcgcaca ggcggcggtg
     3901 gaacacatcg cagacaaaaa ataagcaatg ccgtctgaaa caggattcag acggcatttg
     3961 aacaaacgcg gcaatcaagc tgtcggttca gaaaaaccaa catcggcacg cctaaaaccg
     4021 tattcacaac ctgctccttt tcaaaacatt tgcatttaaa agccgttata atgccgtctg
     4081 aacatctgcc cgaccacatt atgcgtgaat gtcggcagat tgttttcttt tgtaaactta
     4141 tattaaaatc cacttaccga ttcacgccat gccgcccatc cctgccccat ccgcaccatc
     4201 cgagcacact gtcgcatggg tattcggcca acccgttacc gatttgccgc aggatttgtt
     4261 tatcccgccc gacgcgctga aagtcgtatt gggcagtttt caaggccctt tggatttgct
     4321 gctctacctt atccgcaagc agaacatcga tgttctcgat attccgatgg tgaagattac
     4381 cgagcagtat ctgcactaca tcgcccaaat agaagcctat cagtttgatt tggcggcgga
     4441 atacctgctt atggcggcca tgctgattga aatcaaatcg cgcctgctgc tgccgcgtac
     4501 cgaaaccgtc gaagacgaag aagccgaccc gcgtgccgag ttggtgcgcc gcctgctggc
     4561 ttacgaacag atgaagctgg cggcgcaggg tttggacgcg ctgccccgag ccggacggga
     4621 tttcgcgtgg gcttacctgc cgctggaaat tgccgtcgaa gccaagctgc ccgaagtcta
     4681 tattaccgac ttgacgcaag cgtggctggg tattttgtct cgggcaaaac acacgcgcag
     4741 ccacgaagta atcaaagaaa ccatctccgt gcgcgcgcaa atgacggcaa tcctgcgccg
     4801 tttgaacgga cacgggatat gcaggtttca cgacctgttc aatcccgaac agggcgcggc
     4861 ttacgtggtc gtcaacttca tcgcactgtt ggagcttgcc aaagaaggtt tggtcggaat
     4921 cgtacaggaa gtcggtttcg gagaaatccg aatcagcctc aatcatgagg gggcacattc
     4981 agacggcatt tccggcacac ggggcgggcg cgatgtgttc taatacgccc caagccgcca
     5041 ccaaaaatcg ggagacacgc catatgaccg gcatcataca ttcgctgctt gacaccgacc
     5101 tctacaaatt cactatgctg caagtggttc tgcaccagtt tccgcagacg cacagccttt
     5161 acgaattccg ctgccgcaac gcctcgaccg tctatccgct tgccgacatc aaggaagact
     5221 tggaagccga actcgacgcg ctctgccggc tgcgcttcac ccacgacgaa ctcggctatc
     5281 tgcgctccct gcgtttcatc aaaagcgact ttgtcgatta tctcgaactc ttccagctcc
     5341 aacgccgctt tgtcgaaatc ggcacagacg ataaagaccg tctgaacatc cgtatcgaag
     5401 gtccgatgat acaggcgatg ttttttgaaa tcttcatcct cgccattgtc aacgaacttt
     5461 acttccgccg gctggaaacc cctgccgtta tcgaagaagg tgaacgccgg cttcaagcca
     5521 aagccgcgcg cctcaaagaa atcgccgccg cacaaaaccc cgacgatccg cccttcctga
     5581 tttccgactt cggcacgcgc cgccgctaca agctcgcgtg gcaggaacac gtcatccgca
     5641 ccctgctcga agccgcccca agcatcgtgc gcggcaccag caatgtcttt ctcgccaaaa
     5701 aactcggcat tacccccatc ggcaccatgg cgcacgaatt cctccaagcc ttccaggccc
     5761 tcgacgtacg cctgcggaat ttccaaaagg ccgcgctcga aagctgggtg cacgaatacc
     5821 ggggcgattt gggcgttgcc ctgaccgacg ttgtcggtat ggatgccttc ctgcgcgatt
     5881 tcgacctcta tttcgccaaa cttttcgacg ggctgcgcca cgacagcggc gacccttacg
     5941 tttggggcga caaagcctac gcccactatc aaaagctcaa aatcgacagc cgcaccaaaa
     6001 tgctgacctt ctccgacggg ctggacatcg aacgctcttg ggcattgcac caatatttca
     6061 aagaccgctt caaaaccggc ttcggcatcg gcaccaacct caccaacgat atggggcata
     6121 cgcccttgaa tatcgtcttg aaactggtcg aatgcaacgg gcagtccgtc gccaagctgt
     6181 ccgactctcc gggcaaaacc atgaccaaca acagcacctt cctcgcctac ctgcgccaag
     6241 tgttcgacgt acccgaaccc gaaacgccgt aaaccggcag aaaaagcgca caattcctgt
     6301 ttctgccgca taaaatcttt taaaataccg cctgatttga atttaaccga aagaccgaac
     6361 ttcatgaacc tacatcaaac cgtcgaacac gaagccgccg ccgcctttgc cgccgcaggc
     6421 atcgccgaca gtcccgttgt tttgcagccg accaaaaatg ccgaacacgg cgatttccaa
     6481 atcaacggcg tgatgggtgc ggcgaaaaaa gccaaacaaa acccgcgcga actggcgcaa
     6541 aaggtcgccg acgcattggc aggcaacgcc gtcatcgaaa gcgcggaagt cgccggtccc
     6601 ggttttatca acctgcgcct gcgccccgaa tttctcgctc aaaacattca tgcggccttg
     6661 aacgacgcgc gtttcggcgt ggcaaaaacc gacaaaccgc aaaccgtcgt tatcgactat
     6721 tcctcgccca atctggcaaa agaaatgcac gtcggccatc tgcgttccag catcatcggc
     6781 gacagcattt cgcgcgtgtc agaatttatg ggcaataccg ttatccgtca aaaccacgtc
     6841 ggcgactggg gtacgcaatt tggcatgttg gtcgcttatt tggtcgagca gcaaaaagac
     6901 aatgccgcgt tcgagctggc ggatttggag cagttttacc gcgccgccaa agtgcgcttt
     6961 gacgaagacc ccgcctttgc cgacaccgcg cgcgaatacg ttgtgaagct gcaaggcggc
     7021 gatgaaaccg ttttggcatt gtggaaacag tttgtcgata tttcgctctc gcacgcccaa
     7081 gccgtttacg acacgctggg cttgaagctg cgccccgaag acgtggcggg cgaatcgaaa
     7141 tacaacgacg atttgcagcc cgtagtcgat gatttggttc aaaaaggtct ggcagtcgaa
     7201 gacgacggcg caaaagtcgt gttcttggac gagtttaaaa acaaagaagg cgaacccgcc
     7261 gcatttatcg tacaaaaaca aggcggcggc ttcctttacg cctccaccga tttggcgtgt
     7321 ctgcgctacc gcgtcggcac gcttcatgcc gaccgcctgc tgtacgtcgt cgaccaccgt
     7381 caagccctgc actttgagca actctttacc acttcccgca aagcaggcta tctgccggaa
     7441 aacgtcggcg cggcatttgt cggcttcggc acgatgatgg gcaaagacgg caaaccgttc
     7501 aaaacacgca gcggcgacac tgtaaaactg gtcgatctgc tgaccgaagc cgtcgaacgc
     7561 gccaccgctt tggtgaaaga gaaaaatccc gaattgggcg cggacgaagc cgccaaaatc
     7621 ggcaaaaccg tcggcatcgg cgcggtcaaa tacgccgact tgagcaaaaa ccgcaccagc
     7681 gattatgtgt tcgactggga cgctatgctc tcgtttgaag gcaacaccgc cccctatctg
     7741 caatacgcct acacccgcgt gcaaagcgtg ttccgcaaaa caggcgaatg ggatgcggac
     7801 gcgccaaccg ttttgaccga accgctggaa aaacagcttg ccgccgagct gctgaaattt
     7861 gaagacgtac tgcaaagcgt ggcggacacg gcgtatccgc actacctcgc cgcctacctc
     7921 tatcaaattg cgaccctgtt cagccgcttc tacgaagcct gtccgatact caaagccgaa
     7981 ggcgcaagcc gcaacagccg cctgcaactg gcaaaactca ccggcgacac gctgaaacaa
     8041 ggcttggatt tgctgggcat cgatgtgttg gacgtaatgt aaaaccgcac cgcccgattg
     8101 cggacaacag ccccgccatc ctcatccgaa tctgaagaaa gcggcgcgat acaccgtatc
     8161 cgccgtccct cccaaaatgc gaaacaaaca aacgccaagc aagcaagcaa gcaagcaagc
     8221 aagcaagcaa gcaagcaaaa aattataacc cccttcctgc cgacgcacgc actttccgcg
     8281 cggcgcattt cccttttccc gcccctcaaa cccgcctttt cttcaggcag ggtttcagcc
     8341 cgcccccttt ccctgttttc ctttccccga cacgcgcgcg cgacccctgc cgcatcgtgc
     8401 cgcactttcg cgcccggacg gcatcgttcc gccatccggt tctctgtttt aaatacccct
     8461 gtttcagaaa gaaatgcaga tgcttcaaca cacaggacga catataaagc gtcgccctat
     8521 atgttgccct gatttggaag gggttacgcc tcccaaataa agcctgatcc tgccgccccg
     8581 aaggacagat gtccgagtgg cgaagtttca accgaaaagg aaatacgatg aatattcaca
     8641 ccctgctctc caaacaatgg acgctgccgc cattcctgcc gaaacggctg ctgctgtccc
     8701 tgctgatact gcttgccccc aatgcggtgt tttgggtttt ggcactgctg accgccaccg
     8761 cccgcccgat tgtcaatttg gactaccttc ccgccgcgct gctgatcgcc ctgccttggc
     8821 gtttcgtcaa aattgccggc gtattggcat tttggctggc ggttttgttt gacgggctga
     8881 tgatggtgat ccagcttttc ccttttatgg atctcatcgg cgccatcaac ctcgtcccct
     8941 tcgtcctgac cgcccccacc ccttatcaga taatgaccgg gctgttgctg ctgtatatgc
     9001 tggcgatgcc gtttgtgttg cagaaagccg ccgccaaaac cgacttccgg cacattgccg
     9061 tctgcgccgc cgttgtggcg gcagccggct atttcaccgg ccatttgagt tactacgacc
     9121 gggggccgat ggccaatatc ttcggcgcaa acaacttcta ttacgccaaa agtcaggcga
     9181 tgctctacac cgtcagccag aatgccgact ttattaccgc cggcctggtc gatcccgtct
     9241 tcctcccctt gggcaatcaa cagcgtgccg ccacgcatct gaacgagccg aaatctcaaa
     9301 aaatcctctt tatcgtcgcc gaatcttggg ggctgccggc caatcccgaa cttcaaaacg
     9361 ccacttttgc caaactgctg gcgcaaaaag accgtttttc ggtttgggaa agcggcagtt
     9421 ttcccttcat cggcgcgacg gtcgaaggcg aaatgcgcga actgtgcgcc tacggcggtt
     9481 tgcgcgggtt cgcactgcgc cgcgcgcccg acgaaaaatt tgcccgctgc ctccccaacc
     9541 gtttgaaaca agaaggttac gccacctttg cgatgcacgg cgcgggcagt tcgctttatg
     9601 accgcttcag ctggtatccg agggcgggct ttcaagaaat caaaaccgcc gaaaacctga
     9661 tcggtaaaaa aacctgcgcc attttcggcg gcgtgtgcga cagcgagctg ttcggcgaag
     9721 tgtcggcatt tttcaaaaaa cacagcaaag gattgtttta ctggatgacg ctgaccagcc
     9781 acgccgacta tcccgaatcc gacattttca accacaggct caaatgcacc gaatacggcc
     9841 tgcccgccga aaccgacctc tgccgcaact tcagcctgca cacccaattc ttcgaccaac
     9901 tggcggattt gatccaacgc cccgaaatga aaggcacgga agtcatcatc gtcggcgacc
     9961 atccgccgcc cgtcggcaac ctcaatgaaa ccttccgcta cctcaaacag gggcacgtcg
    10021 cctggctgca cttcaaaatc aaataacaac aatgccgtct gaacgcacca acagccttca
    10081 gacggcattt tgcagacaga ccgacccttc aagcccactt ttttcatcat ctccgataaa
    10141 ttgct
//
LOCUS       DAXHSJ010000060        10046 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000060 DAXHSJ010000000
VERSION     DAXHSJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.060"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..571
                     /locus_tag="ACFM8U_001089"
     CDS             <1..571
                     /locus_tag="ACFM8U_001089"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6334340.1"
                     /translation="FHFRKTEKECAVLGTLSRLDTVRAVHLMLDILKKMVDRGMPVRL
                     NIAGIGEEMDNLKTQAKRLGIEDKITFLGGIRDLTGYFKEIDILVNTPHCVGDHGAGV
                     GNNILEAGLYDTPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLRE
                     AMGKRLHAHVAALCSDDEIYRTTMAAYTM"
     gene            591..1759
                     /locus_tag="ACFM8U_001090"
                     /pseudo
     CDS             591..1759
                     /locus_tag="ACFM8U_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1752..2279
                     /locus_tag="ACFM8U_001091"
                     /pseudo
     CDS             1752..2279
                     /locus_tag="ACFM8U_001091"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            2328..3329
                     /locus_tag="ACFM8U_001092"
     CDS             2328..3329
                     /locus_tag="ACFM8U_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFC6334341.1"
                     /translation="MPYFTGNQTNEKNNILILSAGRRVELVQDFQTEAARFSDGISVF
                     ATDLNPRMSSACHVSDGSFEVPPISADSYIDSIFNLAVRENIGLIIPTIDTELQKLAD
                     ERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRSPEIYDRKKLVFPCFAKPY
                     DGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFSEFTVDMYYDRQCRLKCAI
                     PRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCITVQFFYNEETGEFYGVEI
                     NPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWENNLIMLRYDAKVLVHEND"
     gene            3316..4003
                     /locus_tag="ACFM8U_001093"
                     /pseudo
     CDS             3316..4003
                     /locus_tag="ACFM8U_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            4051..4245
                     /locus_tag="ACFM8U_001094"
     CDS             4051..4245
                     /locus_tag="ACFM8U_001094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334342.1"
                     /translation="MTKYYSWGVNGFPPYVFDTIWKRWKLNGRGGGYRVRHPGRHNRR
                     GQSGKAAAAQKPRDLDSISD"
     gene            4369..5544
                     /locus_tag="ACFM8U_001095"
     CDS             4369..5544
                     /locus_tag="ACFM8U_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC6334343.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPNTKAVIVVHLAGMPAEMDGIMTLAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKSFDDTPWRPKERLKNAVELGDTSLMFLVHPTLTDDEIAFCKKHIEAVLAEATR"
     gene            5592..7502
                     /gene="pglD"
                     /locus_tag="ACFM8U_001096"
     CDS             5592..7502
                     /gene="pglD"
                     /locus_tag="ACFM8U_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HFC6334344.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAPLTIGLFVRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQIREYSAVAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIISKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAVEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPDITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            7558..8850
                     /locus_tag="ACFM8U_001097"
     CDS             7558..8850
                     /locus_tag="ACFM8U_001097"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC6334345.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(8902..9738)
                     /locus_tag="ACFM8U_001098"
     CDS             complement(8902..9738)
                     /locus_tag="ACFM8U_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC6334346.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIRAGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYAFLAERFGMLASAGSDFHRLNDFSSGILGACPELPENCRPVWAHFS
                     RH"
BASE COUNT         2438 a         2971 c         2495 g         2142 t
ORIGIN      
        1 atttcatttc cggaaaaccg aaaaagaatg cgccgtactc ggcacgcttt cccgtttgga
       61 caccgtccgc gccgtgcatc tgatgttgga tattttgaag aaaatggttg accgaggtat
      121 gcccgtccgc ctcaacatag cgggcatagg agaagaaatg gacaatttga aaacgcaggc
      181 gaagcgtctg ggcattgaag acaaaattac cttcctcggc ggcatacgcg acttgaccgg
      241 ctacttcaaa gaaatcgata ttttggtgaa cacgccgcat tgcgtgggcg accacggtgc
      301 aggtgtcggc aacaatattt tggaagccgg cctttacgac acgcctgtcg tgacctacga
      361 catggcgggt atttccgaaa tggtcgtcaa cggagaaacg ggttactgca tccccttcgg
      421 cgacgaagaa gcctttatcg ccgccgtaga cacgctcgtc agacagccgc tgttgcgcga
      481 agcaatgggc aaaaggttgc acgcacacgt cgccgcactc tgttccgacg acgaaatcta
      541 ccgtaccacc atggcggcgt ataccatgta atgccgtctg aaagccgccg atgaacatca
      601 ccatagtcgc cccttattgc tcgctgccgt ccgaaccgta tttcaaccgc ttttggtatc
      661 tggcagaacg attgtcgcaa tcgcacgacg tgttgctgat taccagccgt ttccgccact
      721 acgacaaatc cttcagacgg cccgaagatg ccgaggccgc ctcgcaaggc cgtctgaaag
      781 tcatgctgtt ggaagaaagc ggctacggca aaaacgtgtc tttagaacgt gttgccagcc
      841 attaccgctt cgtcaaaaat tttgagcaat ggctgaaaaa ctgccggccg ggcgaacaag
      901 acatcgtcta ttccgcctat ccgctgatgg caaccaacct gctgttgggc aaacacaaag
      961 cgcgtttggg ctacaaactg attgtcgatg tgcaggacgt atggccggag tctttctcct
     1021 cggtcgtacc gtttttgaaa aaagtaccgc acaagctgct gccctttgct tcacgcgcca
     1081 accgtgccta ccgctgcgcc gacgcgctgg ttgccgtatc gcagacttat ctcgaccgcg
     1141 ccaaagaagc caatccgaac gtacccggcg aagccgtcta tatcggtacg gattttgccg
     1201 ccatcgcccc ccccccccgc gattccgctc caaaaccgtc cgccttttct acttgggcac
     1261 gctcagttac agctatgacg tggaaaccgt gtgcaaaggc gttcggaaac ttttagacga
     1321 cggcgaaaac gtcgaattgc acattatggg cggaggaccc gatttggaga aactcaaaca
     1381 atatgaaaat cgcgccatca agttttacgg ttatttaccc tattctgaaa tgatgtcgat
     1441 tgccaaagcg tgcgacattg ccgtcaacgc catccatagc cacgccatgc agtcggttac
     1501 caacaaactc tccgactata tggctttgca aaaaccgatt ctgaacagtc agaacaatgc
     1561 cgaagtcctc gacctcatga atctgctgcc gcacgaaaac taccgttccg gcgatgtgga
     1621 cggtttcgtt caagccgcca aagatatttt gaagcgcaaa aacgatcctg ttcagtccga
     1681 agaaatcgtc cgccgcttca agcgcgacat ttcctatcaa aaaatcgtca acctgattga
     1741 aagattggca aatgagtaaa gccgtcaaac gcctgttcga catcatcgca tccgcatcgg
     1801 gactgattgt cctctcaccc gttttcctga ttttggcgta cctcatccgc aagaatctgg
     1861 gttcgcccgt cttcttcatt caggaacgcc ctggaaagga cggaaaacct tttaaaatgg
     1921 tcaaattccg ttccatgcgc gacgcgcttg attcagacgg cattccgctg cccgacggag
     1981 aacgcctgac accgtttggc aaaaaactgc gtgccgccag cttggacgaa ctgcccgaat
     2041 tatggaatgt cttaaaaggc gagatgagcc tggtcggccc gcgcccgctt ttgacgggct
     2101 acctgccgct ttataacgac ttccaaaacc gccgccacga aatgaaaccg ggcattaccg
     2161 gctgggcgca ggtcaacggg cgcaacgcgc tttcgtggga cgaaaagttc gcctgcgatg
     2221 tttggtatat cgaccattac agtttttggc tggatatgaa aatcctgttt ttgacaatca
     2281 gaaaagtctt gattaaagaa ggcatttcgg cggaagggga agccactatg ccttatttca
     2341 ctggaaatca aactaatgaa aaaaataata tactgattct gtcggcaggt cgccgtgtcg
     2401 aattggtgca ggacttccaa accgaagccg cacgtttttc agacggcatc agcgtttttg
     2461 ccactgacct caacccccgc atgtcttctg cctgccatgt ttcggacggt tcatttgaag
     2521 tgccgccgat tagtgccgat tcatatatcg acagcatttt caatctggcg gtacgcgaaa
     2581 atatcggttt aatcatccca accatagaca ccgaactgca aaagctggca gacgagcgcg
     2641 aacgctttga agcggcaagt gtccacatcg tcgtttccga ttcggacttc atcgcacaat
     2701 gccgcgacaa acggaaaacc gccaccttgt ttgcccgcta cggcatacgc tcacccgaaa
     2761 tttacgaccg caagaagttg gttttccctt gcttcgccaa gccttatgac ggcagccgcg
     2821 ccatcggtgc aaaacggatt gacacacctg ccgacctgac accggaagtc ttggcagaca
     2881 ccaaattaat gttctgtcag tttatcgaca tcgaaaataa attttccgag tttaccgtcg
     2941 atatgtatta cgaccgtcaa tgccgtctga aatgcgccat cccgcgaaaa cgcttggaag
     3001 tgcgctccgg cgaaatcagc aaaggcgtta cctgcaaaaa cagcctctac caaacgctgc
     3061 tggaaaaaat gtcggtattg cagggcgcgc gcggctgtat cacggtgcag ttcttctaca
     3121 acgaagaaac cggcgaattc tacggcgtgg aaatcaaccc ccgcttcggc ggtggcttcc
     3181 cgctgacgta tgccgcaggc ggcaattatc cgagccggtt gatgcgcgaa tacctcggcg
     3241 acgaagaaat cccgttttca gacgactggg aaaacaacct cattatgttg cgctacgatg
     3301 ccaaggtctt ggttcatgaa aacgattaat cctgaaacca ccgtcatcgt cttcgacttg
     3361 gacgataccc tgtattcgga atacgaatac aagctctccg gtatccgttc ggtcatcgat
     3421 accgttgccg cactgtatcc cgattggaat tcagacaacc tatggcgcaa catcgacccc
     3481 gacggcaaag attggctgga caaattgtgc cgccactgtg gttttaatga atcggaaaaa
     3541 caggttcttt ctgtggcaat accggctgca ccgcccgacg ctgactccgt atgctccgcc
     3601 ggatttcctg tccgaactga ccgcgccctt cgccgcacgc gcgctgatta ccgacggcag
     3661 aagcctgacc caacgcctga agctggaagc cttgggtctg tcttccctgt ttgacgacat
     3721 cctcatctct gaagcctgct cttcggaaaa gcccgacggc aaacgcttcc gccatcttca
     3781 agacaaatat gccgacaagg caggctgctt catctatatc ggcgacaaca tttccaaaga
     3841 ctttatcgcg ctgaacactt tgggctggat aaccatagga ttgcttccct ccggacagaa
     3901 catccaccgc caccagcctg aaagttttaa cacagaatat catcccgact tttgggttaa
     3961 ctcccttcaa gatttagttc ctataattga ttcaaatact taaccattac ataatcaaac
     4021 atgcactcac aatattaaaa ggactatttc atgacaaaat attattcatg gggcgtaaac
     4081 ggctttccgc cctacgtttt cgacaccatt tggaaacgat ggaagttgaa tgggcgcggg
     4141 ggcggttatc gtgtgcgaca tcccggacgg cataaccgtc gcgggcaatc cggcaaagcc
     4201 gctgccgcgc aaaaaccccg agacctcgac agcataagcg attaaaatac acccccgtac
     4261 agaccgattt tgacaacacc tgcggcgcgc gcccgattct tcggaacacg cccccttcag
     4321 acggcatagg gtcggaaatg ccgtctgaaa accgacggac aaaccatcat gctgaacact
     4381 ttcctttccc cgtggccctg cttcacccaa gaagaagccg atgccgtttc caaagtcctg
     4441 ctgtccaaca aagtcaacta ctggacgggc agcgaatgcc gcgaatttga aaaagaattt
     4501 gctgctttcg ccggcacgcg gtacgccgtc gcccttgcca acggcacgct ggcactcgat
     4561 gtcgcgctca aagcaatggg cataggcgcg ggcgacgatg tgattgttac ctcgcgcacc
     4621 ttccttgcct ccgcatcctg catcgttaac gcaggcgcaa accccgtgtt tgccgacgtg
     4681 gatttgaaca gccaaaacat cagcgcggaa accgtcaaag ccgcgctgac gccgaatacc
     4741 aaagccgtca tcgtcgtcca ccttgccggt atgcctgccg aaatggatgg cattatgact
     4801 ttggcaaaag aacatgattt gtgggtgatt gaagactgcg cccaagcgca cggcgcaaaa
     4861 tacaaaggca aatccgtcgg ctctatcgga cacgtcggcg cgtggtcgtt ctgccaagac
     4921 aaaatcatga ccaccggcgg cgaaggcggt atggttacga ccaacgacaa aaccctgtgg
     4981 gaaaaaatgt ggtcgtacaa agaccacggc aaaagctacg atgccgtgta ccaccgcgaa
     5041 cacgcgcccg gtttccgctg gctgcacgaa agtttcggca caaactggcg tatgatggaa
     5101 atgcaggcgg caatcgggcg catccagctc aaacgcctgc ccgaatggac tgcctgccgt
     5161 caagcgcacg ccgccaagct ggcggaaagc ctaggcaaat tcaaaagtat ccgcttggtt
     5221 gaagtcgccg actacatcgg acacgcgcaa tataagttct acgccttcgt caaacccgaa
     5281 cacctcaaag acggctggac gcgcgaccgc atcgtgaacg aactgaacgc acgcaaagtc
     5341 ccctgctatc aaggcagctg ctccgaagtc tatttggaaa aatctttcga cgacacgcct
     5401 tggcggccga aagagcgttt gaaaaatgct gtcgaactgg gcgacaccag cctgatgttc
     5461 ttggtgcacc cgacgctgac cgacgacgaa atcgcgttct gcaaaaaaca catcgaagcc
     5521 gtattggcag aagccacacg ataacccttc agacggcata tgccgcctga aagcacatac
     5581 cgccccacga tatgactctg gaaaccctga tcgccctgcc gcgcaacatc aagaaaatct
     5641 gtttcctcat acacgatttt ctgatgattt tcattgcatt ctggttcacc caaagcctaa
     5701 aggccgacta ctcgaacgaa tggttcgacc ctgccaactg gcagtctttc ctattgaccg
     5761 cgccgttgac catcggcctg ttcgtcagaa tggggctcta ccgtgccgtt acacgcttca
     5821 tcagcttccg catcctctcc accgcgctgg caggcagcct cgcctccgcc gtattgttct
     5881 tcctcaacac attgattttt gaagaaaggc tgcgcctcgc cctgccgatt gtctatttct
     5941 tactgctgtt tgtttccgtg accggctcgc gtatggtttt gcgcggactg ttgtccgaac
     6001 accccaaaaa acagatgatc cccgtcatca tttacggcgc gggacggtcg ggcagacaac
     6061 tgcttgaggc cgtcaaacag atacgcgaat attccgcggt cgcctttgtg gacgacaacc
     6121 ccaagctttg gcacaccgtc atctacgacc ttgccgttta ccagcccgat gccatcgcct
     6181 tcctcatcga acgctacggc gtggaaaaaa tcctgctcgc catccccggc gcgacccagg
     6241 aacaacgccg ccgaatcatc agcaaactgg aagcctatcc ctgcgaagtg ttgaccattc
     6301 ccggaatgaa agacctgatg gacgggaaaa tcagcatcgg cacgctcaaa aaaatctccg
     6361 tgtccgacct gctcgggcgt gattccgtcg cgcccgacaa ccgcctcatg agtgccgaca
     6421 tcgaaggcaa aaccgtcatg gtaaccggcg cgggcggctc catcggttcg gaactctgcc
     6481 gccagattat ccgccgccgc cccgaaaagc tgctgctgtt tgagttatcc gaattcgccc
     6541 tgtacgccgt cgaaaaagaa ttgcgcgaaa cctgcatcca aaaacgcctc gacaccgaaa
     6601 tcctgccctt tctcggttcg gtgcaaaacc gcacgctgct cgaacacgtc atgaccgcct
     6661 tttccgttgc gaccgtctat cacgccgctg cctacaaaca cgtccccatg gtcgaattca
     6721 acaccgtcga aggcatacgc aacaacattt tcggcacact cgagtgcgcg cttgccgcca
     6781 cgacatcggg cgtaagaact ttcgtcctca tctccaccga caaagccgtc cgccccacca
     6841 acaccatggg tgccagcaaa cgcatggcgg aactctgcct tcaggcactc gccgccgaac
     6901 ccggacaaaa aacccgcttc agcatggtac gtttcggcaa tgttttaggt tcgtccggct
     6961 ccgttgtccc gctgtttgaa aaacagattg cagaaggcgg cccgcttacc ctgacccacc
     7021 ccgacatcac acgttatttc atgaccatac ccgaagccgc ccaactcgtc atacaggcag
     7081 gcgcgatggg tacgggcggc gacgtattcg tcctcgatat gggcgaatcc gtcaaaatca
     7141 tcgaccttgc ccgccaaatg attaccttaa gcggcctcaa acccaaaaca cccgaacaac
     7201 ccgacggcga catcgaaatc ctcattaccg gactgcgtcc cggcgaaaaa ctctacgaag
     7261 agctgctcat cggcgacaac gtccgcaaaa ccggccatcc gcgcattatg acggctgacg
     7321 aggcgatgct gccgtggcac gagctctctg tcctgctcga ccgcatccgt gcggcctgcg
     7381 accgttacga ccagcaggca atccgcaccc tgctcatcaa cgccccgacc ggctttaccc
     7441 caagcgacgg tatctgcgac ctgctttggg tacgagaaac acacagaaaa aatgccgtct
     7501 gaaccttcag acggcataac gtacaaacca acctgcctga cacacacgga gtttgatatg
     7561 cagttttcgg cattcggcga aaaattcacg caacacagcg gcatcctgca actgatggac
     7621 gacctcggcg acgcgctcaa aagcgacaag cccgtcaata tgctcggcgg aggcaacccg
     7681 gcacgcattc cggaaatcga tcgggcgttc gccgacatat tctccaaact ggcggcggaa
     7741 cacgccgtcg aaaacatcgg caactactcc aatccccaag gcgatgccgc gctgattgac
     7801 gcgctgaccg ccttcctcaa ccgcgaatac ggctggaatt tgaccgccga caatatcgcg
     7861 ctgaccaacg gttcgcaaaa cgcgtttttc tatttattca acctcttcgg cggcaaattc
     7921 aagctttcag acggcacatc cgcagaaaaa gccattttgt tgccgctcgc gcccgaatac
     7981 atcggctatg ccgacgtaca tgtcgaaggg cagcacttcg tttctgtcaa gcccaaaatc
     8041 gaaaacgtcg aacacgaagg cgaagcaggc ttcttcaaat accgcgtgga ctttgacgca
     8101 ctggaaaacc tgcccgaact caaagcgggc aaaatcggcg cgatttgctg ttcgcgcccg
     8161 accaacccga ccggcaatgt gttgaccgac ggcgaaatgg cacgtttgga cgctttggcg
     8221 cgtgaacacg ggattccgct gattatcgac aacgcctacg gaatgccgtt ccccaacatc
     8281 atttacagcg acgtaacgct gaattggcac gaaaacatca tcctctgctt cagcctttcc
     8341 aaagtcggcc tgccgggcgt tcgcaccggc atcattgtcg ccgcgcccga agtcgtcaaa
     8401 gccgtcagca gcctgaacgc gattgtgaac cttgccccca cgcgcttcgg cgcggccatc
     8461 gccgccccgc tactggaaag cggcgagatg aaacggcttg ccgaccaagt catccggccg
     8521 ttttaccgca atcaggcgca aaccgccgtc tcgctgctca agcgcgagct gggcgcgtac
     8581 ccgatgaaaa tccacaaacc cgaaggcgcg attttcctgt ggctctggtt tgaaaaccta
     8641 cccgtttctt cgcaaaccct gtacgaaatg ctcaaagccg aaggtacgct gattattccg
     8701 ggcgagcatt tcttcgtcgg catcgacaca caggattacc cgcacgcggg cgagtgcatc
     8761 cgcatgagca tcgcgcagga cgctcaaacg ctggaaaaag gcattgccgc catcggtaaa
     8821 accgtccgaa aactgtacga caacgtttaa accgcaaaaa atgccgtctg aaagcctttc
     8881 agacggcatt tttatccgca ttcaatgtcg ggagaaatgc gcccacaccg gcctgcagtt
     8941 ttccggcagt tcgggacacg cgccgaggat gccgctgctg aagtcgttta aacggtggaa
     9001 gtcgctgccc gcactggcga gcatgccgaa gcgttctgcc aaaaatgcgt agttgaggcg
     9061 gtcgtttttg cagcagttgc cgctgtggac ttcgatgccc gcgccgccga ggtttttaaa
     9121 ttcttcaaac agattgcgct tggcggtagc ggacaaatca tagcgcatcg gatgggcgat
     9181 gactgccatg ccgcccgcgc cgttgacggc ggagacgcag tcttccagcg tcgcccattc
     9241 gtgtcggacg gcgcaggatt tgccgtcgcc caagtatttg gtgaacgcct gctgcttgtt
     9301 tttgacatgt cccgcccgga tgaggaactc ggcgacgtgg gtgcggctga ccatttcttt
     9361 gtttgccgcc agcgcgagcg cgccgtcgta tgcgccgccg atgcctttct tttcgagctt
     9421 ggcggcgatg gcttcgaggc gtttcagacg gccttgccgc acttgcgcca acaggttttg
     9481 caggttttcg tcttgttcgt caaagtctaa gccgacaacg tgtatggtac gcccgcgcca
     9541 cgttacggag atttccacgc cgttaatcag gcgcaaaccg agcctgtcgg cttcggcacg
     9601 cgcttcggcg atgccgccgg tgtggtcatg gtcggtcaac gccagcagcg tgcagccgtt
     9661 ttgatgcgcg aggcgcacga cttcggcggg ggaaagcata ccgtcggaaa cggtggaatg
     9721 gcagtgcagg tctatcatgg ggtgttatat gtgtggtgaa tgaaggtcgg ggggcaatat
     9781 tgtcgggcag tgtggcggcg gcggtcattt taaaaaacag gtgtatggca aatgcaaaga
     9841 aaaagtccct ataccgtcat tcccgcgcag gcgggaatcc agactttgat ttattggaaa
     9901 tgtttaaggt taaccgctat ttcaaacttc cggattcccg cctgcgcggg aatgacgata
     9961 gggatgtttt caattttaat ctactataaa agactgtctg aaaacgtggt tttatagtga
    10021 attaaattta aaccggtaca gcgttg
//
LOCUS       DAXHSJ010000061         9877 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000061 DAXHSJ010000000
VERSION     DAXHSJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.061"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(313..546)
                     /locus_tag="ACFM8U_001099"
     CDS             complement(313..546)
                     /locus_tag="ACFM8U_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC6334347.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(712..912)
                     /locus_tag="ACFM8U_001100"
     CDS             complement(712..912)
                     /locus_tag="ACFM8U_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC6334348.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1084..1959)
                     /gene="xerD"
                     /locus_tag="ACFM8U_001101"
     CDS             complement(1084..1959)
                     /gene="xerD"
                     /locus_tag="ACFM8U_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC6334349.1"
                     /translation="MEEGLIDRLLETLWLDRRLNQNTLNGYRRDLEKIARRLSQSGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVQHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2011..2451)
                     /locus_tag="ACFM8U_001102"
     CDS             complement(2011..2451)
                     /locus_tag="ACFM8U_001102"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC6334350.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2772..3710
                     /locus_tag="ACFM8U_001103"
     CDS             2772..3710
                     /locus_tag="ACFM8U_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC6334351.1"
                     /translation="MSIHTLKHLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYSSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3839..4132
                     /gene="hfq"
                     /locus_tag="ACFM8U_001104"
     CDS             3839..4132
                     /gene="hfq"
                     /locus_tag="ACFM8U_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC6334352.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            4217..4783
                     /locus_tag="ACFM8U_001105"
     CDS             4217..4783
                     /locus_tag="ACFM8U_001105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6334353.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLSYGFTNRKNNPPYLLAFEKLRQK"
     gene            4837..5211
                     /locus_tag="ACFM8U_001106"
     CDS             4837..5211
                     /locus_tag="ACFM8U_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC6334354.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5221..5715
                     /gene="folK"
                     /locus_tag="ACFM8U_001107"
     CDS             5221..5715
                     /gene="folK"
                     /locus_tag="ACFM8U_001107"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC6334355.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRL
                     LPDK"
     gene            complement(5842..6321)
                     /locus_tag="ACFM8U_001108"
     CDS             complement(5842..6321)
                     /locus_tag="ACFM8U_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC6334356.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDCKDNAYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVFQI"
     gene            complement(6540..7277)
                     /gene="ubiE"
                     /locus_tag="ACFM8U_001109"
     CDS             complement(6540..7277)
                     /gene="ubiE"
                     /locus_tag="ACFM8U_001109"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC6334357.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7320..7688)
                     /locus_tag="ACFM8U_001110"
     CDS             complement(7320..7688)
                     /locus_tag="ACFM8U_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HFC6334358.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWVYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7808..9427
                     /gene="mnmC"
                     /locus_tag="ACFM8U_001111"
     CDS             7808..9427
                     /gene="mnmC"
                     /locus_tag="ACFM8U_001111"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC6334359.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHNTEQTELLLTGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLNTPYAEPLCGLFWQHGVW
                     LNPPAFVRALLRHPLIGLHEDTPLTGISHDGEKWIASTPNGIFSATHIVYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKYQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(9541..9658)
                     /locus_tag="ACFM8U_001112"
                     /pseudo
     CDS             complement(9541..9658)
                     /locus_tag="ACFM8U_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
BASE COUNT         2640 a         2996 c         1987 g         2254 t
ORIGIN      
        1 ctaccgatta cacaaacaca tatcatcttt acacaatcat gcttccatca acagtaaaac
       61 atgatatgat tgccaacaat aacatctcac aataaatttt ctaattttta ttgaaaaaat
      121 caataaataa gaattctccc aaccgacaaa taaaataaag aaagggttaa tatgcaacat
      181 cgaagaagac tagcaattta ccaagcatcc aaacgtgctt cctttaccgg caggtcatcc
      241 gccccgcaaa aacgtaaaga acgttaattt gaaaaaaatg ccgtctgaag tcctgcttca
      301 gacggcattt ttttaccgtt cgagaaactg tttcaacctg tcctcatcca aaaaccagtg
      361 acagatttcc tcatctcccg ccaacaaaac gggaaccagc tcattgtatt tttcttccaa
      421 aacaggattt tcatccacat cgaccacttc cagcccgaac ccgtattcat tttgaaaagg
      481 tttgagtgca tcgcgcattt tgtggcacaa gctgcaatat tcacgaaaca tcaaggtcaa
      541 tttcatccgc gtttccttat ctgtcaattt gcacacgcca aaaccttaga cgcagcagaa
      601 tcatggtcta tttgggaaaa aacaatgttt tcaaggaaga tgatactcaa gtcctgccaa
      661 aaacagcaaa aatgccgtct gaacagttca gacggcattt ccaaaaccgt tttacgcttg
      721 aacattgata cccgccgtaa ccgtcggttg cgcattgtgc gccgtcagaa acgaagcggc
      781 aagctcccca cagcagccgc agcagctcgc tacgcccaat tgcgactgca aatcgcccat
      841 tgtggtcgcg ccggcggcga tggtttcctt gatttgatgg tcggtaacgg cattgcagat
      901 gcagacaaac attttgtgct ccgtgtgttc tcaaactatc gtaccgatag cggcttttat
      961 tatcgtatgc aaatataaat aaaaacggtt cgcattgcaa ggtcggcata cacggtttgt
     1021 cttggtaatt ttttatcaag ttttcatttc cgattgattt cttattaaac cgaagcagaa
     1081 gcctcagttt cgggaatggt gttccttcac tacaccctgc aaccatacat tggcaacatg
     1141 ggtgtaaatc tgcgtcgtat tcaaatcggc atgtcccaac atatcctgaa ccacgcgcaa
     1201 atccaatccg tgctgcacca agtgcgtggc aaaggcgtgg cgcaggctgt gcgggctgat
     1261 gtgcccgatg cccgccctac ctgcatattc tttgacaatc atccatgcca actgacgaga
     1321 aatgcccgtc tttttctgac tgacaaacaa tgcgtcgcaa ttcctgcctt tcagcagaag
     1381 tgggcgtgcc tccgtataat agcgttccac ccaatacgcc gactcctgcc ccatcgggac
     1441 catcctctgc ttatcgccct tgcccagcgc ggtaatacag cccctgtcca aatccacatt
     1501 gccgaagttc agcccgaccg cctcgctgac gcgcaagccg gtcgcgtaca tcaattcgag
     1561 cagtgccttg tcccgcaaac cgtgtggcgt atcagtatcc ggagtggcaa gcagtcggga
     1621 aatctgctgc tcggtgatca gggtcggaat attcttgtcg attttgggcg gtttcagcaa
     1681 acgggtggga ttgtccgtcc ttatgccttc acgctccatc catatataca ggcgtttgca
     1741 cgccgataat gcgcgcgcct gcgaactccg ttgctctccg tcaacataaa ccgccgccgc
     1801 caaatccgct tcgtccgcat ccttcagcgt tctgcccgat tgggacaggc ggcgggcgat
     1861 tttttccaaa tcgcgccggt aaccgtttaa agtattctga ttgagccgcc tgtccaacca
     1921 cagcgtttca agcagcctgt cgatcaaacc ttcttccata ccgttccaaa caaatgccgt
     1981 ctgaatcttc ttcaggcggc atggttcaca ttatcgggaa agcgtttcca acacttcctg
     2041 tgcatgaccc gccactttga ctttccgcca ttcatgggcg atttctccat ccttattcaa
     2101 gacgaacgta ctgcgctcga tacctaacga ctctttcccg tacagtttct tcaatttgat
     2161 gacatcaaac aggcggcaca ctgtttcatc cttgtcgctc aacagctcga accggaaacc
     2221 ctgcttggcg caaaaattct gatgcgcctt tacgccgtcg cgggaaatac cgaccacggt
     2281 ataacccaat gcctcaaact gttccaaacg cgcattgaaa tccaagcctt ccgtcgtaca
     2341 gccaggcgta ctgtctttcg gataaaaata cacgaccaaa ggcagatgtt ctgccgaatg
     2401 aaaatccgca ccgctgctcg aaggcagggt aaattcatat ttcacatcca tagtcctact
     2461 cccgatattc ccattattca aaacggcacg cagacgcacc gccgcaattg ccaaaccaac
     2521 cccgattcta ccgccccaaa ggacaaggat tcaaccgccg gaaacatcca aaccgacaca
     2581 cgacggcatg aaaaatatcc atgtcaaacc acaaaatatg ttccgattta aaaacagaat
     2641 gttataaaac ccaatcccca aaacacaaca agaccgcccg cctacgggca gtctcctgtc
     2701 agacgacata ctttacagat ggctgttttt tcaacaaaat aacgccaata ctcaaaaata
     2761 tggaatcaaa aatgtccatc catactctga aacacctgcc ctcatcgctg ctgctcggtc
     2821 tctgcctttc cctgccgtca gcccaccttt ttgccgacaa cgacatttta gggcaatttt
     2881 tagaacagaa catgcttacc tcctccgatc cgatagaaat attcgccgaa agcacgatac
     2941 accccaccaa cacccaagcc attacaggcg gtctgattct ctcctcacag tctgccctgg
     3001 tcgtcaacaa caaaaccgga cagatactgt atcagaaaaa cgccgacagg attatgccca
     3061 tcgcctccat ttccaaactg atgagcgcga tggtcgtttt ggatgcaaac ttggacatga
     3121 acgaaaccgt taccattacg cccgacgaaa tcgaccgcat caaagggacc ggcagccgtc
     3181 ttgccatagg tacggcactt acacgcaaag aactgctgca cctgagcctg atgagcagcg
     3241 aaaaccgcgc cacccatgca ttgggcagaa cctaccccgg cggcatgggc gcatttgtcg
     3301 ccgctatgaa ccgcaaagcc caaagcctgg gcatgtacag cagccgcttt tacgaaccga
     3361 ccggactcaa cttccaaaac gtttccaccg ccaaagacct gagccttatg gtcaacgccg
     3421 ccgcccaata tccgcaaatc cgcaccaact cgacttccaa ctacgcctcg gtacagacca
     3481 aaaacgggca gcagaactac aaaaactcca atgcgctggt cagggaaggc atgtggaaca
     3541 tcgaattgca gaaaaccggc tacatacgcg aagcaggcag gtctatggtt gtcaaagcca
     3601 acattcaaaa tcaacccgtt accatcgtat tgctgaactc gcccacatcc gccacacgcg
     3661 tcaacgacgc ccgcaaaatc gaatcgtgga tgctgcagca acgctcctga catacaaatg
     3721 cccggcggaa aaccgcccaa acacaaaaaa aattggtaat ttttcctatt caaggctaca
     3781 attcgacacg cacagggcgt ctgcccgatt tcacaatcat aatagcggag ttaaaaatat
     3841 gacagctaaa ggacaaatgt tgcaagatcc cttcctgaac gccctgcgta aagagcatgt
     3901 tccggtttcg atttacttag ttaacggtat caaattgcaa ggtcaggttg agtctttcga
     3961 tcaatacgtt gttctcctga gaaacacttc cgtcacccaa atggtttaca aacacgccat
     4021 ttccaccatc gtaccggcac gctccgtcaa cctacaacat gaaaacagac cccaagccgc
     4081 accgacttcg accctcgtcc aagtggaaac cgtccagcag cctgccgaat aatccgcacg
     4141 aagcatgacg tgtcatatct ttcaatacct taccggacaa cggtaaggta tttttatttt
     4201 cagacagcat ttaaaaatgt tattgcaaaa catccttcca ttcgcccatt gccttttgcg
     4261 gaaggcactt cccgaaggtg gcaatgcttt ggacggcacc gccggcaacg gacacgacac
     4321 ccttttcctc gcacaaaccg caggcatccg ggggaaagtg tgggcattcg acatccagcc
     4381 gcaagccctg agccgcactc gatgccgtct gaaagaagcg agatacgaga atgtgcaact
     4441 catcttagac ggacacgaaa acctgaaaca atatgtttca gaaccactaa atgccgccat
     4501 tttcaatttc ggctggctgc ccggcgggga caaaagcctt accacccgca cggaaaccag
     4561 cattgccgcc ctttctgcca ccttatccct actgaaagaa aacggtatgc ttattgccgt
     4621 cctctatccg ggacacgaaa acggcaaaca ggaggcagaa gcaatcgaac aatgggcaaa
     4681 aaacctgcct caagaacagt ttgccgtttt gagttacggc tttaccaacc ggaaaaacaa
     4741 cccgccctat ctgttggcat ttgaaaagtt acgtcaaaaa taaatgtttg cggtaaaata
     4801 agccgattct aacccttatt ttgatacgag ccgaccatgc aatatctgat tgtcaaatac
     4861 agccatcaaa tcttcgttac catcaccatt ttggtattca acatccgtgt tttcctactt
     4921 tggaaaaatc cagaaaagcc cttggtcggc ttttggaagg cactgcccca ccttaacgac
     4981 accatgctgc tgtttacggg attgtggctg atgaaaatta cccatttctc cccgttcaac
     5041 gcgccttggc tcggtacaaa aatcctgctt ctgctcgcct atatcgcatt gggtatgatg
     5101 atgatgcgcg cccgtccgcg ttcgaccaag ttctacactg tttacctgct cgccatgtgt
     5161 tgcgtcgcct gcatcgttta ccttgccaaa accaaagtcc tgcctttctg aaacaccgtt
     5221 atgaacaaca gacattttgc cgtcatcgcc ctgggcagta atcttgaaaa ccctgcccaa
     5281 caggtacgcg ccgcattgga cacgctgtcg tcccatcctg acatccggct tgaacaggct
     5341 tcctcactgt atatgaccgc acccgtcggt tacgacaatc agcccgattt catcaatgcc
     5401 gtctgcaccg tttccaccac tctggacggc attgccctgc ttgccgaact caaccgtatc
     5461 gaagccgatt tcggacgcga acgcagcttc cgcaacgcgc cgcgcacatt ggatttggac
     5521 attatcgact ttgacggcat ctccagcgac gacccccgac tcaccctgcc gcatccgcgc
     5581 gcgcacgaac gcagtttcgt catacgccct ttggcagaaa tcctccctga ttttattttg
     5641 ggaaaacacg gaaaggttgc cgaattgtca aaacggctgg gcaatcaagg catccgtctt
     5701 ttaccggata agtaattccg cacgcggatg ccgtctgaat gccggtttca gacggcattt
     5761 ttcctttgcc gccaacacgc gtgcaaaaaa atcgcccctt ggaaaagggg cgcaaaagga
     5821 acacaaacca ctaccaaaac tttaaatctg aaacactgcc tgccgcatac tgtatccgac
     5881 aggatataaa gccctcacta aatcgtttcg agaaatccaa acttcttcat cgccgacaga
     5941 aaatctgcct ttctccggca ccagctccaa caaaaacggt tgaaccgccg tatgcagcct
     6001 gtccttacac cgccccagct ttctggacaa cgcggacagg gcgcgtttgt aggcattatc
     6061 cttgcagtca agctcccgcg cactgccgac ccagctcaaa cgaaggttgc ggctttcatc
     6121 ctcaatcagc aaaatgcctg ttttgacttc ccccacctcg ggctcgcatg aaagcgcaat
     6181 ataatatttg ccgtcgatgt tgacatacgc cgcttcatgc acctgacagt tgcagccgga
     6241 agtggcaagc agcgaataaa cagaatcccg atgcagtttc aaaatatcaa gggaaatctg
     6301 tttgaaaata ctttccggca ttttccctcc tttcgtctgc tgcgttgttt actggattga
     6361 ctgcattata cagacatctc ccaatcttgc aaatgataat tgttcctatt ttttaaaaag
     6421 cttataaatt attgatatat atgtaaatta atttttaaga tttaaaacct ttgtctcaag
     6481 aaacacaaaa atgccgtctg aaacgtgttc agacggcatt ggctgcacag gcagtccgtt
     6541 taaaatttca cgcccttatg cagcgcgacg atgcccgcac tcatattgtg ataatccacg
     6601 ctgtcaaagc ctgcatccag catcatctgt ttcaaagttt cctgatcggg gtgcatacgg
     6661 atggattcgg caagatattg ataactctcc gcatctttgg caatcagcct gcccatgacc
     6721 ggcagcagct tgaacgaata gaaatcatac gcgccttcca aaggtttgta gattttggaa
     6781 aactccaaca ccagcaacgt gccgcccggt ttcaaaacac ggtacatctc tttcagcgcg
     6841 gcatctttat gcgtcatgtt ccgcaagccg aacgccacgg aaaccaagtt gaaataattg
     6901 tcggggaaag gcagtttttc cgcatcggca agcgatacgg gcaaaatcat gccttcgttc
     6961 aacagacggt cgcgcccgac ggtcagcata gaggaattaa tatcggtcag ccaaacctcg
     7021 ccttccttgc cgacccgttt cgcccaaccg cgcgacaaat cgcccgtacc gcccgcaatg
     7081 tccaacactt tatcgccttt tttcaggtgc gccgtgttga tggtgaaatg cttccacacc
     7141 ctgtgcagcc ctgccgacat cacatcgttc ataatgtcgt agtttttggc gacggagtgg
     7201 aacacttccg ccacttttcc ggctttctca tcttcgttga ccgtactgaa tccgaaatgc
     7261 gttttttgtc cgcccatctt ctgaccacct ccctgatgcc gtcctccggc atcccaaact
     7321 taacaatctg attgcttttc aaaacgcgac gcaccagccg ccgccaattt gtccaaatat
     7381 tcctgccaca tcgcatcgta tccgtatgcc agtctgtgca gatacaccca atcgtaaaga
     7441 ccgctgtcgt gcccgtccga aaaactgatt ttcagcgcat actgtccgac aggctgcaaa
     7501 tccgtgattt ggacatccgc cttgccggtc tgcaaaacat cctgtcccac gccgtgtccg
     7561 cgcacttccg cactcggcga atagacgcgt aaaaattcgg caggcagatt tttaggttcg
     7621 tccccataag tcaaaaccaa cacggtacgg tttttcaaca aacggatttc aacaggaata
     7681 ttattcaaaa aacacctcgg ttcggcatac aatatccggc aggcgcaggc gtgccttcaa
     7741 acggcgcgta ttttaacagc tttatcccga taccgatatt caaacccaca tttcagacgg
     7801 cattgatatg gacaatctcg tatgggacgg cattcccgac atccgcacac tcgaccaaac
     7861 catccgcaaa cacgcacacc cgctcaacct gattgtctgc ctccccgata atcagattcc
     7921 cgattttcaa accgcacaag atgcttcgga cgcggaatgc cgtctgaagc accgtttgga
     7981 tcaggcaacg cagtgcctcc agttcgacag catcaacctc atcgaacaca tcctgcccga
     8041 tgtccgcttc tggctggttc ccccttcacg cacccgccgc ctgcacgaac acttccacca
     8101 catttcctgg cagaccgaag ccatcccgca aaccgaaagt aagcccgaca aaccctggtt
     8161 tgcacttcca caaacatccg aacggcaaaa accggaacac atcctcgtta tcggagcggg
     8221 catatccggc gcggcaaccg cccacgcctt agcatcatac ggcatttccg ttaccgtatt
     8281 ggaagcccga aaagccgccc aagccgccag cggcaaccgc caagggctgc tctacgccaa
     8341 aatctcgcca cacaataccg aacaaaccga acttttgctc accggctacg gctacaccaa
     8401 acgcctgctc ggacacatcc tgcccgaatc cgaaacctgg ggcggcaacg gcatcatcca
     8461 cctcaattac agccgcaccg aacaacaacg caatcacgaa ttgggtttgc aaaaacacca
     8521 taaccacctc taccgcagca tcacgcaggc agaagccgaa aaaatcgccg gtatcccgct
     8581 gaacacgccc tacgccgaac cattatgcgg actgttttgg cagcacggcg tatggctcaa
     8641 tccacccgca ttcgtccgcg ccctcctccg ccatccgctg attggactac acgaagacac
     8701 accgttaacc ggtatttccc acgacggaga aaagtggatt gcaagcacgc caaacggcat
     8761 cttttctgcc acacacatcg tctactgcac cggcgcgcac agcccctgcc tgcccgaaac
     8821 caacctcgcc gccctgcccc tcaggcaaat acgcggacaa accggcctca caccgtccac
     8881 cccgttttcc gaacaactgc gttgcgccgt ttcaggcgaa agctacatca gcccgtcgtg
     8941 gcacggactg cactgctacg gcgcgagttt tattcccaac agcagccata ccggatggaa
     9001 cgaagccgaa gaagcctcaa accgccaagc attggcacac cttaaccccg cccttgccga
     9061 atcattgttt gccgccaacc caaaccccca aaaataccaa gggcacgccg ccatacgctg
     9121 cgacagcccc gaccaccttc ccctagtcgg cgcactcggc gacattgccg ccatgcggca
     9181 gacctacacc aaactcgcgc tggacaaaaa ctaccgcatc gacaccccat gcccatacct
     9241 gcctaatgcc tacgtcaaca ccgcccacgg cacacgcggg cttgccaccg cccccatctg
     9301 cgccgccgcc gttgcagccg aaatcctagg cttgccccat cccctctcaa aacgcctgcg
     9361 ccacgcccta caccccaacc gcaccatcat ccgcgccatc gtcagaagga aggatctaac
     9421 cccttaagcc tttgccgcat gccgtcccga ccacagaaaa tcatattcaa ataaacatac
     9481 caatcctgac ggtaaaatat cgaaaacacc cataacgttt tcttcccaaa ataaggagta
     9541 aggaatgcct aacaaattgc ctttgctgtc caagatgaat gttccgtaac attcgaagac
     9601 ttccttaacc ctgccagccg tttcggtttc tgtaccccct gttagataag gaaggaatga
     9661 aaaagccctg ttgattgtat gaagtaaagg gcgttaattt ttgttaatct tcccttctca
     9721 ggaactcaat atataaggat gttaattttt atttcccttc tcgaggactc aatatagtgg
     9781 attaacaaaa accagtacgg cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc
     9841 ttcatcgcct tgtcctgatt tttgttaatc cactata
//
LOCUS       DAXHSJ010000062         9877 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000062 DAXHSJ010000000
VERSION     DAXHSJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.062"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            205..1254
                     /locus_tag="ACFM8U_001113"
     CDS             205..1254
                     /locus_tag="ACFM8U_001113"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6334360.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRIGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(1323..2864)
                     /locus_tag="ACFM8U_001114"
     CDS             complement(1323..2864)
                     /locus_tag="ACFM8U_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC6334361.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(3475..4830)
                     /gene="xseA"
                     /locus_tag="ACFM8U_001115"
     CDS             complement(3475..4830)
                     /gene="xseA"
                     /locus_tag="ACFM8U_001115"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC6334362.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            5027..5848
                     /gene="nadE"
                     /locus_tag="ACFM8U_001116"
     CDS             5027..5848
                     /gene="nadE"
                     /locus_tag="ACFM8U_001116"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC6334363.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGMYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(5918..6391)
                     /gene="dnrN"
                     /locus_tag="ACFM8U_001117"
     CDS             complement(5918..6391)
                     /gene="dnrN"
                     /locus_tag="ACFM8U_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC6334364.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPIGVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            6689..7021
                     /gene="trxA"
                     /locus_tag="ACFM8U_001118"
     CDS             6689..7021
                     /gene="trxA"
                     /locus_tag="ACFM8U_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC6334365.1"
                     /translation="MSSELIVHTGDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(7074..8021)
                     /locus_tag="ACFM8U_001119"
     CDS             complement(7074..8021)
                     /locus_tag="ACFM8U_001119"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6334366.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            8349..9737
                     /locus_tag="ACFM8U_001120"
     CDS             8349..9737
                     /locus_tag="ACFM8U_001120"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6334367.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
BASE COUNT         2415 a         2729 c         2593 g         2140 t
ORIGIN      
        1 ttgaaagatt ctacccccgc acgcaaaccg atttcaaccg ttgcacaaac tttgcctcgc
       61 cacaagccgc agcgacgatt tcatccgcaa aaccgccgca tcaggtacaa tatcgaaccg
      121 tccgactgag gacggcattt tatcaacccg tcctgccgca cacgccgcag aagaaccgcc
      181 ttatcaggcg agttaggaaa aatgatgtcc aaacagccca ccagcaaacg ccaatggcgc
      241 gacggcgcag ccccgtctgc caagaaaacc gccaaaccgt tcaaaagcaa agcccgtccc
      301 aaagacgaaa cggacaaaac cgcatcccaa ccttacggac aaaaagcttc agacggcatc
      361 aaacctcaaa acctccccaa acagcgcgcc gccaaagcca aaaaactcgt cgtccgcaat
      421 cccaaccaaa aaattatgga acacgcgcgc gatttaaaag aacgccgcag cgacctatcg
      481 cgcatggaac ccgaacgcct gcaaaaagtg cttgccgcgt ccggcgtcgg ctcgcgccgc
      541 gaaatggaag aatggatcaa caacggctgg gtaacggtca acggcaaaac cgctcaactg
      601 ggcgacaaag tcacccccga cgaccacgtt accgtcaaag gcagcatcat caaactcaaa
      661 tgggccgacc gcctgccgcg catcatccta tattacaaac aagaaggcga aatcgtttcc
      721 cgcgacgacc cgcaaggccg cgtcagcata ttcgaccgcc tgccgcaggc cgccagcagc
      781 cgctgggtcg ccatcggacg cttggacatc aacaccagcg gacttctgat tctcaccacc
      841 tccggcgaac tcgtccaacg tttcgcccac cccagcttcg aagtcgaacg cgaatacgcc
      901 gtgcgcgtac tgggcgggct gaccggcgaa caaatgcgcg tcctcaccga agaaggcgtg
      961 atgctcgaag acggcttggc aaaagtcgaa cgcatccgcg aacaaggcgg cgaaggcgcg
     1021 aacaaatggt acaacgtcgt gattaaagaa ggccgcaacc gcgaagtgcg ccgcattttt
     1081 gaaagccaag gactcaccgt cagccgcctc gtgcgcatcg gcttcggtcc catcggactg
     1141 cccaaccgcc tcaaacgcgg acagttctac gaactcaacc ccgccgaagt cgccaacatc
     1201 atcaaatggg cggacatgct gctgccgggc gaacgccgcc gcaaaaaagc ctaaacccgc
     1261 caaaacacaa aaatgccgtc tgaaacatct gctgtttcag acggcatttt attcgggcgt
     1321 tttcaggaga aaaggtcgag tgctttgaca aagaccatca ccacgccgta ggcgagcggt
     1381 atgacggcca atgcccaacg ccaccagacc gatatgccgc cgccggaaac tttttcgccc
     1441 acaaggtaag cgtcggatat ggcggtttcg tcgtcggggt tgccgctgtg tgcggcggtt
     1501 ttgatgtctt tttcgtggtg tttttcgtgt acggatttga cggcgaggtt gcacaacaaa
     1561 ccgataatca gcaggcacgc catgatgtac atggttacgc tgtatgcctg tgccgccggt
     1621 atgccgctgt cgatttggct ttggcgtatg taattgacca gtaccgggcc gatgacggcg
     1681 gcggttgacc aggccagcag gatgcgtccg tgaatcgcgc cgacctgata ggtgccgaac
     1741 aggtctttca ggtaggcggg aatggcggca aatccgccgc cgtacatgga aataatcacg
     1801 caaaagccga tgatgaacag ggctttgctg ccgccctcgc cgatggaggg aacggcgaaa
     1861 tacagcagcg agccgagtac gaagaagatg gtgtaggtgt ttttgcgtcc gattttgtcg
     1921 gaaacgctcg accacaaaaa gcgtccgccc atgttaaaca ggctcaggag gctgacgaag
     1981 cctgccgccg cacctgcgcc gactgctgcc tgcctgccta tggaggtttc ggaaaagagt
     2041 tcctgaatca tcacggatgc ctgacccaat acgccgatgc cggcggttac gttcaggcac
     2101 aatacccaga acaacagcca aaactgcggc gttttcatgg cttgggacac gttgacatga
     2161 ttactgctga ccagcttgtt ttgcgttttc ggcgcggtat agccttcagg tttccagccg
     2221 tcggcaggta cgcggatggt aaacgcgccg aacatcatca gtgcgaggta aagcaggccc
     2281 aatacggcaa aggtttcggc aaccccgacc gaagcggcgt ttgaaaaggc gttcatcagt
     2341 gatacggaaa gcggagaggc cagcattgcg ccgccgccga aacccataat cgccaaaccg
     2401 gtcgccatac ccggcttgtc gggaaaccat ttcatcagtg tggaaaccgg cccgatgtag
     2461 cctaagccta agcctacgcc gccgatgacg ccgttgccca aatagagcag gaagaggttg
     2521 tgcgtacgca cgccgaatgc ggatacgaag aagcccaggc tgaaacagca ggcggcggca
     2581 aatatggctt tgcgcggacc tacccgttcc atccatgtgc cgaacagggc agccgacgcg
     2641 cccagcatcg cgagtgcgat actaaaaatc caacctacgg tcgtcagctt ccaatctccg
     2701 gccgccgatt cggttatgcc gataagtttg gtcagcggcg cgttgaatac ggaataggcg
     2761 taaatctgcc cgatggcaag gtgcaccgcc aatgctgcgg gcggtacgag ccaacggttg
     2821 aaacccggct tggcaatgct tgcctcacgg tctaaaaact tcataacatc ctctttctgt
     2881 cagttgaaaa ataaaatttc atttgctcaa tggaaactta ttgaaaatta taaaaaaata
     2941 tcgggtcagg tttttatccg ccccaagatg cgccgtctga aacatttcgg gtgtacggaa
     3001 aggtttctgt tttttccgac aaattcctgc ggcttttcgc ttccggattc ccgcttttgc
     3061 gggaatgacg aattaaagat tatcttaagg tcaagggact ggattcccgc ttttgcggga
     3121 atgacggcgg cgggttgctg ttttttattt ataaatttct gtaactttgg atttcagcca
     3181 tcttcaataa attatcgaaa cgtatcaatc gtcattcccg cgcaggcggg aatccatcca
     3241 gttcgttcga tttcggtttt ttaagtttcg agtaacttct gactcgtcat tcccgcgtag
     3301 gcgggaatcc ggttttttga gtttcagtca tttctaatac attcccacca cttttcgctt
     3361 ccggactccc gctttttcag gaatgacgaa ttaaagatta tcttaaggtc aagggactgg
     3421 actcccgctt ttgcgggaat gacggcggag ggggagcggt ttttccgatt gggtttaaat
     3481 gcaatcgaac aaatcctgct gcccttgttc tttgcttacg cgcacgtcgg tttcgccgtc
     3541 ggcgaaggta atgtgcagct tctgcccctg cttcaaaaca tcggcgttgc ggatgacttg
     3601 tccgcgtgtg tttttgacga cggaaaagcc gcgctccaga atgtgctgcg gcgaaacggc
     3661 ttcgagcaat gcggcttggg cggtcaggct ttggcggcgg tgggtaagca gttggctgaa
     3721 ggcggcgggc aatgccgtct gaaggcggac gatgttgttt ttgcaaacgg aaacatcagg
     3781 acggtaatgt tgcagggctt gggtttggcg ttcaaaacgg gcggtgtggg cacggaggtt
     3841 ttgcttcata gagtaagaca gcgtttgcgc cagcttgccg attgaagcgc gctgctcatc
     3901 aagtttttgg cgcggatgac ggatttgccg cgcaagccag tcgagttttt ggctggcatc
     3961 gaaatagcgt tgttccaaaa cggttttcag acggccttgc gcctgagcga gacgatgtag
     4021 cgattcttgg cggttggggc tgaccagctc cgccgcaccg gtcggcgtgg gcgcgcgcac
     4081 gtcggcgaca aaatcggcaa gcgtaaaatc ggtttcgtgt cccacgccac tgacgacggg
     4141 aaccgtgcag gcttcgattg cgcgcacgac cggttcttcg ttaaacgccc acaagtcttc
     4201 aatgctgccg ccgccgcgac agacaatcaa cacgtcgcat tcggcgcgtt gcgaggcggt
     4261 tttaatcgct tgggcaattt gtaactcgct gcctgcgcct tgaacaggtg tcggataaac
     4321 gataacggga atttcgggcg cgcggcgttt caaggtggta acgacatcgc gcaatgccgc
     4381 tgctgccagg ctggtgacga tgccgataca ttgcggacgg gtaggcaacg gctttttgcg
     4441 ttccgccgca aacgcgcctt ccgcctgcaa ctgtgccttc aaccgctcgt aggcttcgta
     4501 aagctgcccc aaacctttga gccgcacctc attcacggta atttgaaatt cgccccgcgc
     4561 ctcgtaaata ctgatttttc ctgatacttc gatatggtcg ccttctttca aaggcttcgc
     4621 caaacgcgcc gccgcacctt tgaacatcgc gcaacgcacc tgcgcgcggc tgtctttgag
     4681 cgagaaataa taatgtccgc tggcggcacg ggtcaggttg gacacttcgc ccgcaatcca
     4741 caggccggca aggtggtttt ccaaaagact tttggcaaat gcgttcaact cggaaacgga
     4801 caacacgtca gaatgaaaaa aatcagacat agaatcaatc aaataataaa aaatatgaat
     4861 atgttttgaa gcctaaggcg gtattagtcc gcctaaattg tcaacaatat tataacacgc
     4921 gccatcttgc cgcccgcctt ttcccgtatg acttttttaa gcggggaatg ggaaaaatat
     4981 tcatcaaccc gccctcaatc tattcaaatt acaccgccga caagctatga tgcggatatt
     5041 ttcgacagga ggaaaaatgg atacgcaggc agttatcaca catattgtcc gatggttgga
     5101 cgaatacgcc gcccgggcaa atgcaaaagg gtttgtcgtg ggcgtttccg gcggcatcga
     5161 ttccgccgtc gtctccgcac tcgccgcccg caccggccgc accacgctgc ttctggatat
     5221 gccgatacgc caacaccccg accagcttga gcgggcaagg ctgcacatcc gcaatctgca
     5281 acggcaatat gccaatgtaa gcgcacaaac ggtcgatctg accgacacct tccagacctt
     5341 tgaacaaacc gtcggcgttc atcagacggc atttgacaat cagccgcttt ccctcgccaa
     5401 cgccagaagc cgcctgcgta tgctgaccct gtactactac gggcagatac acggactact
     5461 ggttacgggg acaggtaaca aaattgaaga tttcggtgta ggatttttta ccaaatacgg
     5521 cgacggcggc gtggacatca gcccgattgc cgacctgacc aaaacccagg tttaccggct
     5581 tgccgaagca ttgggcgtgg acgaggcgat tcaaaaagcc ccgccgaccg acggcctgtg
     5641 ggatacggaa cgcaccgacg aggaacagat gggcgcgagc tatcccgaac tggagtgggc
     5701 aatgggcatg tacggcacgc gcaaacccga agattttgaa gggcggcagc gcgaagttct
     5761 ggaaatctat acgcggctgc accgcgcgat gcagcacaaa atcaacccga ttcccgtgtg
     5821 ccgcattccg cccgaattgt tgggctgaaa cgtggaaatg ccgtctgaaa cggaaaaccg
     5881 tatttcagac ggcatggaaa tatccgactc ctatccctta agaatcgagt acgcgggcaa
     5941 acaaaatatc gttttccaaa tgaatgtggt cgttcaaatc ctccaccatt tctttcgcca
     6001 gcgcgtaaag ccgcgtccag cttccgcaag ccccttctgg cggttggaaa ttgtcggtca
     6061 gctctttgag ccgtgcgatg gcgcggtcgt gttcttcatg ttcgtgcatc atcacgccga
     6121 tgggcatcgc cgcaccgcgt ccgacaccct gattaatcat cggaaacagc atccgctcct
     6181 ccttcatcat atgcatcagc agttcgttct gcatataggc aagcagcccg gcaatttctg
     6241 ccggaaaggt atcggcatgg acttgggcca ctttctgcgc cagcggcacc aattcttcaa
     6301 attgtgcgcg gtggacattg tggtagcgtt gcaggatatg atcgacggtt gcaccaaagg
     6361 gggcggtctc ccaaacggaa aaatcagtca tcgcagtgtt ccttttacag ggtttcgggt
     6421 ttggttttga acattcatac tttaagaatc aattcaaacg gagcatacac cgcccgcgcg
     6481 cttctgtaca gcctcaaacg tattccttac attttgataa taaaagtaat tttcagaaat
     6541 aaaatactgt ccgaaccgtt ttttagaatt tgcaaaggcg attggggcgg tacagaaaaa
     6601 ctattatccc gcccgcccac ttgaaatttt tatgcccaag ccctatcctg cacgctatcg
     6661 tgccaatccc aaccgaaaag gaaaaataat gagcagcgaa ttgattgtac acaccggcga
     6721 tgccgctttt gaaaaagacg ttttaaatgc agatatcccc gtcctgctgg acttttgggc
     6781 tccgtggtgc ggcccctgca aaatgattgc cccgattttg gacgacattg ccgccgaatt
     6841 tgaaggccgt ctgaaagtgg tcaaaatcaa catcgacgac aacgaagcca ccccgtcccg
     6901 tttcggcgtg cgcggcattc cgaccctgat ggtgttcaaa aacggcgaag tcgtcgccac
     6961 caaagtcggc gcattggcaa aaggtcagct gaccgccttt gtcgaagcct ctatcgcctg
     7021 ataaagcgca atcgaaaaag ccgccggaag attccggcgg ctttttcgca cccttaagat
     7081 ttgtggcgga tttcccagca cctatggatt tttttgttgc ggaaatcttc gggaacggat
     7141 tgtttggaaa tgtctttgac ggcgtattgt tccgatacca agtcgtctaa gacgaagctg
     7201 cgcaggttgt tggaaaagta caaaatgccg tctgaagcga gcagcttcac cgcgccgtca
     7261 atcagctttt tgtggtcgcg ctggatgtcg aggatgtcgg acattttctt gctgttggaa
     7321 aaactgggcg ggtccatcac aatgaggtcg aaccgcctgc cttccccata tgccgtctga
     7381 agatattgga acacgtcggc gcggacgatt ttatgccgtt ccgtatcgat gccgttcaat
     7441 tcaaaattgc gtctcgccca atcgagataa gtgttagaca aatcgacagt ttcgctggat
     7501 gccgcgccgc cggtggcggc atagacagtg aagctgccgg tgtaggaaaa caggtttaaa
     7561 aaacgtttgc ccgccgccgt ttcgccgact tttttgcgcg tgtttcgatg atccaaaaaa
     7621 agccccgtat ccaaatactt atcaaggttg acccaaaact tgcggccgtt ttcggtgatg
     7681 acgaaatcgt cgcccgcctt gccggttttt tcgtattgct gcaaaccttt ttggcgttcg
     7741 cggcgtttga ggcggatttg ttcgggcgca aaaccggtaa cgaaggcgac ggcttccaag
     7801 acttcggcaa gccacgcttc gtattcttcg ggccgcatca gccagccggt atcgtattcc
     7861 tgaaggtgga ttcgatcgcc gtaaacatcg gcggcaaagg ggaattgggg gatgtcgcgg
     7921 tcgtaaatgc gccaggcttc gatgccgttg cgtttcgccc atttcataag gtgtttgatg
     7981 tttttgccca agcggttggc aaacggtgtg atgtcggtca ttggtttcag gcggaataaa
     8041 gtggaaaacg gcaattttac tgtaattaac gcccgattgc ttgaccgttt cgggcaaacc
     8101 ctataccatc caccgcttat cttgtcatac gaagccatcg ccttccaacc taaaccgccc
     8161 ttacgggcgc gtttcttctg ttgctttgat tttgcaaagc gtatctgtgc aggttgccgt
     8221 cgatgtaaac cacaagcaag ccgcttgcga caaccctgta acttcacatt ccccgtatcg
     8281 ttacccttcc ctgcttcagg ccgtctgaac ctttcggacg cgggcgttgt tgtcttccaa
     8341 ggatagccat gtctattaaa tttgccgatt tgaaccttga taaaaacatt ttgtccgccg
     8401 tcagcagcga gggttacgaa agcccgacgc cgattcaggc gcaggcgatt ccgtttgctt
     8461 tggaaggccg cgacatcatg gcttcggcgc aaaccggctc cggcaaaacc gccgcctttc
     8521 tgttaccgac tttgcagcga ctgaccaaac gcagcgaaaa accgggcaaa ggcccacgtg
     8581 ctttggtgtt gaccccgacc cgcgaattgg cggctcaagt cgagaaaaac gcgctggcgt
     8641 atgccaaaaa tatgcgttgg ttccgtaccg tcagcatcgt cggcggcgca tcgttcggct
     8701 accaaacccg cgccctgagc aaaccggtcg atctgattgt cgccacgccg ggccgcctga
     8761 tggacttgat gcaaagcggc aaagtcgatt ttgaacgttt ggaagtgctg attctggacg
     8821 aagccgaccg tatgttggat atgggcttta tcgacgacat cgaaaccatc gtggaagcaa
     8881 cgccgagcga ccgtcagact ttattgttct ccgccacttg ggacggcgcg gtcggcaaac
     8941 tggcacgcaa actgaccaaa gaccctgaaa tcatcgaagt cgaacgcgtg gacgaccaag
     9001 gcaaaatcga agaacaactg ctgtactgcg acgatatgcg ccacaaaaac cgcctgctcg
     9061 accacatcct gcgtgatgcc aacatcgacc aatgcgtgat tttcacgtcc accaaagcca
     9121 tgaccgaagt catcgcggat gaattgtacg aaaaaggttt cgccgccaac tgcctgcacg
     9181 gcgatatgcc gcaaggctgg cgtaaccgca cgctgatgga cttgcgcaaa ggccgctgca
     9241 aaattttggt tgccaccgat gtagccgcac gcggtatcga cgtaccgacc attacccacg
     9301 ttatcaacta cgacctgccg aaacaggcgg aagactacgt ccaccgcatc gggcgcaccg
     9361 gccgcgcagg ccgcacgggt attgcgatta cgtttgccga agtgaacgaa tacgtcaaag
     9421 tccacaaaat cgaaaaatac attaaccgaa aactgcccga actgaccatc gagggcatgg
     9481 aaccgacccg caaacgcaaa tccgcaggcg gcaagccgaa aggcaaaggc ggctggggcg
     9541 atcgtaaatc cggcggttgg cgcggcgatc ataaaccggg caaagaaggc ttcggcggca
     9601 aaacgcgcgg cgaaggtttc aagaaagaag gctttaagaa agacggtttc aaaaaaaccg
     9661 gcgaaggctt caaaggcaaa cgtaaagccg gcgattcttt tgcaggcaaa agcgaacgcc
     9721 gttacaaaga ccgctaagcc ccaacctgcc tcataaacca atgccgtctg aaaccgattt
     9781 cgagtttcag acggcatttt tgcaatgttt caacaccgcc cggctttgat acccaaagga
     9841 ttaatctgca atatagtgga ttaacaaaaa ccagtac
//
LOCUS       DAXHSJ010000063         9781 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000063 DAXHSJ010000000
VERSION     DAXHSJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.063"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(338..4507)
                     /locus_tag="ACFM8U_001121"
     CDS             complement(338..4507)
                     /locus_tag="ACFM8U_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC6334368.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHHPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTGKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4562..6391)
                     /locus_tag="ACFM8U_001122"
     CDS             complement(4562..6391)
                     /locus_tag="ACFM8U_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC6334369.1"
                     /translation="MMIKPTALLLPALFFFPHAYASAADLSENKAAGFALFKNKSPNT
                     ESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAPDNVKTM
                     LRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEYYRNALE
                     NWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADLNVVVDS
                     GRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHYSGASVQ
                     ADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKGYIGSVV
                     WDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVRDRAGID
                     ARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDGKIGTTL
                     GTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTLARDNADVPSGLMFRSGGA
                     SSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDAAANFKR
                     MKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6785..8014
                     /gene="sstT"
                     /locus_tag="ACFM8U_001123"
     CDS             6785..8014
                     /gene="sstT"
                     /locus_tag="ACFM8U_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC6334370.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8126..9409
                     /locus_tag="ACFM8U_001124"
     CDS             8126..9409
                     /locus_tag="ACFM8U_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6334371.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNSAESANQTGNNQPADSSDSAPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLT
                     HCKGDSCNGDNLLDEEAPSKSEFENLNESERIEKYKKDGKSDKFTNLVATAVQANGTN
                     KYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGN
                     IFAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGTAVYNGEVLHFHTENGRPY
                     PTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGR
                     FYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9484..9699)
                     /locus_tag="ACFM8U_001125"
     CDS             complement(9484..9699)
                     /locus_tag="ACFM8U_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334372.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(9692..>9781)
                     /locus_tag="ACFM8U_001126"
     CDS             complement(9692..>9781)
                     /locus_tag="ACFM8U_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6334373.1"
                     /translation="VVTMEKDLRRMLLFFKREAYVVILERDRV"
BASE COUNT         1956 a         2502 c         3028 g         2295 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacctgtgta
       61 tcgttgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccag tacagcgttg
      121 ccttgccttg gctcaaagag aacgattctc taaggtgctc aagcaccgag tgaatcggtt
      181 ccgtactatt tgtactgtct gcggcttcgt cgccttatcc tgatttttgt taatttacta
      241 tatcgattct gttcccttgc aggcggacgg cggcggcgga cggtggaaaa gcaggttttc
      301 aaatgtccgg tttggcggcg cgccgtctga aaaccgctta ttttcctttg ctgtttccgg
      361 cggagtcttt tttgtccgaa ccggagaagc ggtcgaaacg tatggtgtat gtcagctcgc
      421 cgcccgacga acggctgccg atacgggcaa ccgcctgtat ggcgcgggtc agccggtaaa
      481 tcagtttgac ggactgttcc gcgctggaga tgctgtattc gtagccgatg tagagtttgc
      541 cggtcagttg tttgccgacg gtcagcacct gttcggcggg gttgagttcg ccggtttgcg
      601 cgttgcggct gcgcttgctg gtaaagccca aatcatccac cagcccgatg cggtcgttga
      661 tttgcccggc aagcagcgcg ccggcggctg cggacagggc ggcattgtcg ccgctgctgc
      721 cactgccggc gcggttgagg atgagccagg agagcttgtc tttttcactc atcggttcgt
      781 ttgccgtcag cgtaatgcgc ggactgttga ggctgcccaa tatttccacg cccgcaccga
      841 cgggggaaag gcggcgttcg gcgcggatgt tgaggttggg gtcgttgagc gggccgacaa
      901 aggagaccgt gcctttggta atgtccaaat cctgcccgta tgccttataa cgccctttga
      961 tgacgcggac cgtgcccacg ccccgcacgc ttccgcccga ttgggcggtc agggtcagtt
     1021 tgccgcctat ggtaacgtcc gcgccgtagc cggcgaagcg gatgccgtca ttgaggtcta
     1081 aagtcaggtt catattgacg gggagcggtg ccgccgcctc ttttttgact tcgcctaata
     1141 cgacgacatc gtcgccgacg gacggcatcg aggatttttg cgaaccgaac agcccctgat
     1201 ccgttttaat catcccggta acggatatgc ctttttgcgg cgaatagcgc aggcgggtgt
     1261 tgccggaaac cgtcaggcgg cggttggggc gggacaggat gcggtatttg tcgaacaccg
     1321 cgccgatatc gacatcgggt ccgctgtttt ccataccgac cgtaccggag agttccgccg
     1381 tcccttcgtg ccggaatttc aggctgtcga ttacccattt cctgcccgcg atatgcgaac
     1441 gcagcgagcc gttgtccaag atgatgcctt gggtttggtt gcggtaatag agtttgtcgc
     1501 cgttgatgct gccgcccaag tgcggatcgg cgatgctgcc gccgagggtt acggcggcat
     1561 tcaggctgcc tttgacggtt tgtccgaccg gtaggaagtt gcggaatact tcggcatcgg
     1621 caacggtcag gttgagcctg ccgcccaaag gcgcggtatc gaaagagcgg ctttgcccga
     1681 cggtgatgtt gccgttgatt ttcccgtagt tgctgctgcc gttgacggcg gcattgacgg
     1741 acggagagcc tacccgtccg ccgatttgcg cggcggcatt caggctgccg gtaatgtttt
     1801 gcgcggcggc gggcagaaag ggcttcaatg cgcccaagtc gggaagggag gcggtaatcc
     1861 tgccgccgag cggtgcattt gccatattgc cgccgaaggc gttggcgatg cccaaatcgg
     1921 cgttaatccg cccgaaacgc gcgccgccgt caagcaggat tccgatacgg tcgttttgaa
     1981 agcgcgtttt cagggaaaat gcgttcaaac ccaaagcctg cccgccgggc aatacggcat
     2041 cgccgctttg ccggctgata ttgaggtagc cgcgcgcgtt gcgcccgtag gcgacatccc
     2101 agtcgccgtt taaaaccaga ttgtgttcga agggcggttt gaagaaattg tgcaactcgg
     2161 cgatatgcag accgtgtgcg ccgccttttg ccgatatgcc ggttttttta tcccaagaaa
     2221 agtgttgcag gttgaggctg ccgcccattg cctgccaatt tgccgcactt gccgccacgc
     2281 gttccgcacc ggcttcgagc gtcatacggt tttgcagctt gaggttgaat gcgccgccga
     2341 tgtcgaggat gccgatgctg cctttccatc gggtaagttc cctgttgatg ccgcctgaag
     2401 cgtccaaatc gaatttgaac ggtttgccat ccagcgtcat ggcggcgtgt gtgcggatgc
     2461 ggtgctgcac gcccgtgccg tccagcatca ggtcggcggt atcgacaacc gccgctccgc
     2521 ccgacagcga aaggcggctg cctttgatgt cggcgcgtat cgggcggctt gtgtcgggcg
     2581 aacctttgag cgtgaaatcg agcgaacgga tgtctgccgc cttgccgatg tgcaggttgc
     2641 gcgccgcgcc ggaaaggtcg gtttcaaagg ttcggatgcc gccgtccaaa tcgccggaaa
     2701 ggtgtccgcg tacatttaaa gaccccgcga gtccgaaacc gaaacgggat aaatcgggtg
     2761 cggtgatatt gaggttaagc cggtcgcctt ttttgccgaa gccgccgtct gttttaataa
     2821 tgttccgccc cagccgcaaa tcgacggcgg cacgcggaag gtggcgggac tcgtaaacaa
     2881 tgtcggcact gccggcaatc ggtacgccgt tgaacgtgcc gggtaaaaac cgcattttgc
     2941 ctgtgaattt ctcttttgcc agttcgccgg caaggtttat tgagccgttg atattgcctg
     3001 ccggaagttg cggatcgatg cgcgaagggt cgaatgcgcg ggaacggatg tccagcttga
     3061 gcaggcggtc tttaaacagc tcgagatagc cttgcgcggt caggctgcct tgcccggcgg
     3121 cgatgttgac ggtgtcgagc accagtttcc gctgtccgtt tgcggggtcg cttgcaatgg
     3181 cgaggctgcc gtccgtgcgc gccgtgccga tgccgagttg ccaagagatt ttgggcgagg
     3241 cggtcgtgcc accgatgccg atgctgccgt ccaacctgcc tttgaacgcg gtttgcagta
     3301 cgtcttccgc gccgacggag ttgatgccta tatttaaatc gaggatgtct tttccggtgt
     3361 cgattttgcc cgacagcctg atgccgcccc gtccgagcag ggcggcggac gtattgccga
     3421 tatgcaccgt gccgtcctgc cggatgacaa agctgcctaa aacctgacgg acggggatgc
     3481 cgttgcggtc ggcaaaaccg gctttggtgt tttccaaatc gagcgagcct tccagcgcga
     3541 tgccgtctga aaacgacggg atggcggtca ggtcgaaatt cagcccggca tcgggcaggg
     3601 aaggtacgaa ggcggccgga ttgatgttga accctttgac cagtacttct tccaatgttt
     3661 tatccaatga ttcggcaaac gggtggatga cggattttcc cgagaggcgg atattgccgc
     3721 cgtcgatcgc cagttcggcg cgcacatcct tcaggctacc gctcagccga gccgtactgt
     3781 gtatggtttt gccttcgagt ccgcctttgg tgtaaatggc ggtatcgagg gcaaacggtt
     3841 ttttcaagcc gaccgaggcc gcccccgacg aactgctcca cggtgtgtcg gcggccttca
     3901 ggtcaaggcg gtgtcctttg cggtcgtaac ggtatgaagc atccagccgt tcgagataga
     3961 cggtttgttt gtcaaaggct ttgcccatgc tgattttgcc cgtctcgaag cggtcgagat
     4021 agacggcggc aggcaggtct atgctgtcgg gaaggctgag cggcgggcgt tcttctttag
     4081 gcggagtcgg tttggtaacg atggcgatgt cgccggcgga aatttcggta atgtgcaggc
     4141 tgcggcgcat cagttcggac ggtttccacg cgaagcggaa gcggctgatt ttaaggtctg
     4201 ccccctcggt ttctatcgac cagttgtcgc cgtcgaagcc gtcgagcagc gtgcctttga
     4261 ggttttggga ggaaatgttt acgccgaacc aagacgggat ttggtacagc ccgaagcgca
     4321 aacccgcttc cgtgccggcg agccagccga ggaaacatac tgccaaaatc agaacagaca
     4381 gcagtgccgc cgacagcttc agcagcgggc ggcgtttttt tgccggcggg gtagggtggt
     4441 gttcagacgg cattttgcgc gtgccgtttt cggtcggatc ggtatctgtc ggtgcggtat
     4501 cggtcataat gtctgtttca aaatggtttg ctgcaatgcc gtctgaagcg gccgtatcgg
     4561 tttagaagcg tgttcccaag ctgatgtgcc agcggatttt cttatcgctg tgcccgtagg
     4621 cgatgtcgaa ggaaaacggc gcaagcgggc tgaaccagcg cacgcccagt cccgaaccgt
     4681 gtttcagctt catacgtttg aaattggcgg cggcatcgcc catatcgtgg aataccgcgc
     4741 cggaaagggt gcgcgtaaac ggcagttggt attccaggct gcccaccaag agggcgcgtt
     4801 cgggcaggac cgatccgttc gggccggcaa gtccgatgct gtcgagttcg taaccgcgca
     4861 cggaagacgc gccgccgctg cggaacatca gccccgaggg gacatcggca ttgtcgcggg
     4921 caagggtgta acccgcttgt ccgcgtatga tgaacgtgcc gagtttcttg ttttcgggcg
     4981 tgaagaaata acctgcgcgg gcagaggtgc ggatcagcgc ggtggaggac aggaatgtgc
     5041 ccaaagtcgt accgattttg ccgtcgaggt aatggccgtt ttcgggatgc agcacgttgt
     5101 tgagcagctg gcgtttccaa gaggcggtca gcatcgtggc gtggctgttg cccaaatcga
     5161 tatccgagcc ggggattttc cggccttctg cgagaaactc tgcccccagc ctggcatcga
     5221 tgcccgcgcg gtcgcgcaca taccagatgc cgccggagaa ggcgcgtttt tcgaggtttt
     5281 gggtggtcga acggttgtag gaaacgttgc ttgtccagta gttgccccga tagttgcgcg
     5341 gctggctgat gccggcggca agcgtggttt cgtatttgtc catatcccag acgaccgagc
     5401 cgatatagcc tttgttgaag aggttgtaat agtcgtaggc gattttgccg cccaaaccgt
     5461 attccgaatc gaggcggatg ccggtttcga gtttgtggcg tttgacctcg gttacgctga
     5521 ctttgacggg aacgcggtcg ccttggagac ggtcgaagtc ggcttgtacg gacgcgccgg
     5581 aataatgccc gttttgttcg agcgcctgtt ggaagtcgag cagcaggtcg aggtcgtagg
     5641 gcgtgcccgg ttggaagcgc gccaggccgg agacgatttg ttcggggtaa cgctgcgtgc
     5701 cggtgatttc aaagtcgccg aaggcgatgg ggcggccgct gtccacgacg acattcaaat
     5761 cggcggtggc ggtatcgggg ttgacggccg cccgggtgtt gccgagtttg gcaagcgggt
     5821 agcctttgcg cgttaccgcg ccgaggacgg aagttttgct gttttcccaa ctgtcctgat
     5881 cgaaatcgct gcctaccggc tgctgccagt tttccagcgc gttgcggtag tattcggcga
     5941 ggttgccgtc tgaaaggatg tcgccgagga tggcgacgcc gacgttggcg attttggtgc
     6001 gcgggcccgg tgtgatgtgc accgtataag ctccgtcttt ttccgtcagg ctgaccttgc
     6061 tgctgaaata gcctttgctg cggagcattg ttttaacgtt gtccggcgct tcttcggcga
     6121 ggaagcccgt ctgttccttg tccaatactt cttcctgctg ctgcgtgatg agcggcaggt
     6181 gttcttcgac catatctttg atttcactgt cctgcgtgtc gatgcggacg ggaaatttgg
     6241 gttttaattt gactgattcg gtgttggggc ttttgttttt gaacaatgcg aaacccgccg
     6301 ccttgttttc ggaaaggtcg gcggcagacg cgtatgcgtg cggaaagaaa aataaagccg
     6361 gcaggagcag ggcggtcggt ttgatcatca tggtacgggt gtcgggtatc ggtgtggcgg
     6421 ctttcgattt taacactgtt cggacggggc gggacgcttc agacggcata ccgccgccgg
     6481 tatttcggag gttgcggccc acctggaatg tatctgcttg ttttaaggta ttttgtttcc
     6541 aatccgcata tggaaaacag gctgcgtttc ggaatagtgt cgaaaaataa acgggattaa
     6601 ataaatttat tttcgttcaa taaattaaac cgttgcgccg aatgataaag caaagcctgt
     6661 ccggcactgc atagggtaaa tcaacgcaac cttaaaacac agccgaaaaa taaaaaaaac
     6721 acagagaatg agttttcttt taaaatacaa cattttttaa catatcacaa cataaggaag
     6781 aattatggcg ttcggcaaat ccttatttca tgcgatcggc cgcgtcagcc tggtcaggca
     6841 gattgccgcc ggtttggcgt tgggtatcgt aatcggttcg gtttccccgc aactgggctt
     6901 ggcggcaggc ttgttcggca gcctgtttgt cggcgcactc aaagcggtcg cgccggtttt
     6961 ggtatttatt ttggtggcgg ccacaatcgc gcagcaccaa aaaggcaaca aggcgcatat
     7021 caggccgatt atcgtccttt acctcatcgg tacgttttcc gcagccctga ccgccgtcat
     7081 cgccggtatg gttttcccga cgcacattgt tttggcgggc gcgggcgatg tgtccgccgc
     7141 gccgccttcc ggcattgtgg aagtattgaa atcgctgctg atgaacctgg tcgccaaccc
     7201 gattaacgcg attgccaatg ccaactatat cggcattttg gcttgggctt tggttttggg
     7261 cgcggcgttg cggaatcacg gttcggacgt tacgcggcag gtcgttgccg atttggcgga
     7321 agcggtttcc accgtcgtga aatggattat ccgttttgcc cctttgggca ttttcgggct
     7381 ggtgtcgtcc acaatcgcgg agacgggttt cggcgcgctg gcgggttatg cgaagctgct
     7441 tgcggtactg ttgggctgta tggcgtttat cgcgctggtg gtcaatcccg ccatcgtgtg
     7501 gtggaaaatc cgccgcaacc cttatccgct ggtgttcacc tgcctgcgcg aaagcggcgt
     7561 gtatgccttc tttacccgtt cttccgccgc caatatcccc gtgaatatgg ctttggcgaa
     7621 aaaactgggg ctgcacgaag acacttattc gatttccatc ccgttggggg cgaccgtcaa
     7681 tatgggcggc gcggcgatta cgattaccgt tttggctatg gcggcggcgc acacccaagg
     7741 catacaggtt gattttgcca ccgcgctgct gttgagcctg gtggcgacgg tcagcgcgtg
     7801 cggcgcgtcc ggcgtggcgg gcggttcgct gctgctgatt ccgctggcgt gcagcctgtt
     7861 cggcatcagc aacgatgtcg ccatgcaggt ggttgccgtc ggctttatca tcggcgtgat
     7921 tcaggattcg gcggaaacgg cgttgaattc ttcaaccgat gttttgttta ccgctgccgc
     7981 cgatttgggc cgacagagaa accgggcgga ataaaccaag ctatgccgtc tgaagcccgt
     8041 ttggcgttca gacggcatat ttttacaatt ttatgggcat atggcggaaa caataccggc
     8101 tgccttgaat atgaagggga tgatgatgtt tgaacgcagt gtgattgcaa tggcttgtat
     8161 ttttgccctt tcagcctgtg ggggcggcgg tggcggatcg cccgatgtta aatcggcgga
     8221 cacgctgtca aaaccggccg ctcctgttgt tgctgaaaaa gagacagagg taaaagaaga
     8281 tgcgccacag gcaggttctc aaggacaggg cgcgccatcc acacaaggca gccaagatat
     8341 ggcggcagtt tcggcagaaa atacaggcaa tggcggtgcg gcaacaacgg acaaacccaa
     8401 aaatgaagac gagggaccgc aaaatgatat gccgcaaaat tccgccgaat ccgcaaatca
     8461 aacagggaac aaccaacccg ccgattcttc agattccgcc cccgcgtcaa accctgcacc
     8521 tgcgaatggc ggtagcaatt ttggaagggt tgatttggct aatggcgttt tgattgatgg
     8581 gccgtcgcaa aatataacgt tgacccactg taaaggcgat tcttgtaatg gtgataattt
     8641 attggatgaa gaagcaccgt caaaatcaga atttgaaaat ttaaatgagt ctgaacgaat
     8701 tgagaaatat aagaaagatg ggaaaagcga taaatttact aatttggttg cgacagcagt
     8761 tcaagctaat ggaactaaca aatatgtcat catttataaa gacaagtccg cttcatcttc
     8821 atctgcgcga ttcaggcgtt ctgcacggtc gaggaggtcg cttcctgccg agatgccgct
     8881 aatccccgtc aatcaggcgg atacgctgat tgtcgatggg gaagcggtca gcctgacggg
     8941 gcattccggc aatatcttcg cgcccgaagg gaattaccgg tatctgactt acggggcgga
     9001 aaaattgccc ggcggatcgt atgccctccg tgtgcaaggc gaaccggcaa aaggcgaaat
     9061 gcttgctggc acggccgtgt acaacggcga agtgctgcat tttcatacgg aaaacggccg
     9121 tccgtacccg actagaggca ggtttgccgc aaaagtcgat ttcggcagca aatctgtgga
     9181 cggcattatc gacagcggcg atgatttgca tatgggtacg caaaaattca aagccgccat
     9241 cgatggaaac ggctttaagg ggacttggac ggaaaatggc ggcggggatg tttccggaag
     9301 gttttacggc ccggccggcg aagaagtggc gggaaaatac agctatcgcc cgacagatgc
     9361 ggaaaagggc ggattcggcg tgtttgccgg caaaaaagag caggattgat gttgatgccg
     9421 tctgaagcgg gggcgttcag acggcattgt gatgtttggg gtggggtccg gttcaagtcc
     9481 gcatcagttg ataaacccgt cgtctttcat tttcttttcg gaaaaatact ggccgcatac
     9541 cagcagggcg gcgaggatga tgacaaacag ccccgccata gtcagtgtga ttttgaagaa
     9601 caattccgct tcgagaaaag aaaaccaaag ttgaacaatc agcagcagca cccagccggc
     9661 aaacagtccg agacaggcat aaacgccgag cttaaacacg atcccgctcc aaaatgacga
     9721 cgtaggcctc gcgtttgaaa aacagcagca tacggcgcaa atctttttcc atcgttacga
     9781 c
//
LOCUS       DAXHSJ010000064         9756 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000064 DAXHSJ010000000
VERSION     DAXHSJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.064"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            125..1117
                     /gene="pheS"
                     /locus_tag="ACFM8U_001127"
     CDS             125..1117
                     /gene="pheS"
                     /locus_tag="ACFM8U_001127"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC6334374.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            1294..2358
                     /locus_tag="ACFM8U_001128"
     CDS             1294..2358
                     /locus_tag="ACFM8U_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6334375.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            2365..2883
                     /locus_tag="ACFM8U_001129"
     CDS             2365..2883
                     /locus_tag="ACFM8U_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334376.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            2919..5282
                     /gene="pheT"
                     /locus_tag="ACFM8U_001130"
     CDS             2919..5282
                     /gene="pheT"
                     /locus_tag="ACFM8U_001130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC6334377.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     CVFEIARVFSKGSDGQFVQNERIGGLWYGAVMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRVVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            5356..5658
                     /locus_tag="ACFM8U_001131"
     CDS             5356..5658
                     /locus_tag="ACFM8U_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC6334378.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            5642..5950
                     /locus_tag="ACFM8U_001132"
     CDS             5642..5950
                     /locus_tag="ACFM8U_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334379.1"
                     /translation="MTNNVDLKVPVKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(6229..6384)
                     /gene="rpmG"
                     /locus_tag="ACFM8U_001133"
     CDS             complement(6229..6384)
                     /gene="rpmG"
                     /locus_tag="ACFM8U_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC6334380.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(6413..6646)
                     /gene="rpmB"
                     /locus_tag="ACFM8U_001134"
     CDS             complement(6413..6646)
                     /gene="rpmB"
                     /locus_tag="ACFM8U_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC6334381.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(6896..8422)
                     /gene="farB"
                     /locus_tag="ACFM8U_001135"
     CDS             complement(6896..8422)
                     /gene="farB"
                     /locus_tag="ACFM8U_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC6334382.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(8446..9600)
                     /gene="farA"
                     /locus_tag="ACFM8U_001136"
     CDS             complement(8446..9600)
                     /gene="farA"
                     /locus_tag="ACFM8U_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC6334383.1"
                     /translation="MDTHADETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARTQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         2607 a         2308 c         2493 g         2348 t
ORIGIN      
        1 agaaaattta tccaatggat tgaccgtgaa gaaaataagg tcgtctgaag agtctgatat
       61 gtcaggctat acaggcggcc tcgttgtttc aggtggcata tcattaattg acagacttga
      121 tattatggaa aatgtaaacc gcatcgttgc agaaggcatt gccgcagtag aagctgcgca
      181 agacttcaac gctctagaac aaatcaaagc ccgttatctt ggcaaaaccg gcgagttgac
      241 cggacttctg aaaactttgg ggcaaatgtc gcctgaagag cgcaaaacca taggtgcgca
      301 tatcaatgaa tgcaaaaacc ggtttcagac ggcttttaat gccaaacgcg atgccctcaa
      361 cgaagccaag ctgcaagccc gacttgccgc cgaagccctc gacattaccc tgcccggacg
      421 cgctcaggaa ggcggtagcc tgcatcccgt aaccctgacc ttgcaacgtg tggtcgaact
      481 ctttcacgga atgggtttcg aagtggcgga cgggcctgaa atcgaagacg attttcacaa
      541 tttccaagcc ctgaacatcc ctgcaaacca tcctgcccgt gcgatgcagg atacgtttta
      601 cgttgaaaac ggtgatgttt tgcgtacgca cacttccccg attcaaatcc gctatatgct
      661 cgataaaaaa gagccgccca tccgcattat cgcccccggc cgcgtttacc gtgtggacag
      721 cgatgccacg cactcgccta tgttccatca ggcggaaggt ttgtgggtag aagagggcgt
      781 aacttttgcc gacttaaaag cagtgttcac ggattttatc cgtcgcttct ttgaacgcga
      841 tgatttgcaa gtacgtttcc gcccgtcttt cttcccgttc accgaaccgt ctgccgaaat
      901 cgacattatg ggcgaaaacg gcaaatggct ggaagtcggc ggctgcggta tggtgcatcc
      961 caacgtgttg agaaatgtga atatcgatcc cgaaaaatac accggtttcg ctttcggtat
     1021 cggtctcgac cgtttcgcga tgctgcgtta caacgtgaac gatttgcgct tgttttttga
     1081 taacgatttg aattttttga agcagtttgt aaaatgattg tgtaggctgc ctaaaaatga
     1141 aaaaagcagc ctgtgcctac ctcagaaagc taaatcatga aacgctatgt cgctccatta
     1201 tccgcctgct atgcgtttac tatggtgctg tggttgctgt cttttcacaa tgtgtcttgt
     1261 acaaatattg ttgatatata ttagggggat tatatgatta aatcattttg gcttgaaaat
     1321 ttcgggccga ttagcttatt gaaggctgat aatttaggga aaataaattt aattattgca
     1381 gaaaatagca aagggaaaac ttttatttta aaggcattat atagtgtttt aaaaagccat
     1441 gaggaagctc ataaaggaaa gaatattcgt gatttttctg aggaattgcg agataagcta
     1501 tattggacgt tccaagtaga ggaaattggt gatttagtta caagaggaaa agaaaatcca
     1561 aacgaacggc ctcttaagtt aagtatgact ttagaagata gttcttcagt tttgttttct
     1621 tttggtagaa ctactaaaaa gctaattaaa ccggagctat acgaactttc tcccagaata
     1681 aacgctaact ctattttttt accacccaaa gaagttctat cactatttga tgtaattaaa
     1741 aaatcagagg aagaaaagag atttggtttt gatgctactt acattgattt ggtaaaagca
     1801 ttggatatta aaccaacaaa agggaggaat taccctgagg cagcggaagc tagaaaggat
     1861 ctagaagcat tgtttggcgg ttatgttagc tacaatgata aaaagaaggc atgggtatat
     1921 gaaaaagata gacaaacttt ctctatcaat tctacagcag agggggtaaa aaagattggg
     1981 atattagaca ctctattagg aaatcggttt ttgagtcctg gatcaatctt atttattgat
     2041 gagccagaag ctaatctaca tccgaaagca attaatcaat ttctagaaat tctgttttcc
     2101 ttatctaaat ccaaattggg aatacagatt tttatgagta cgcattcata ttttgttatc
     2161 aagaagctgt gtttattagc tagaaagcat agtgaaagta ttcctgtttt tatgcctgga
     2221 aaagaaaagg aacagactac ttgggtgcag gaaaatttac aaaatagcat gccggataat
     2281 gaaattattg gtgagtctat tcgattgttc agagaagaaa taggtgtttt atctccggaa
     2341 acagcatgga ttggttaggt ttttatgagt gaaataataa atctcactga atcaggtata
     2401 acttttaatc tagtaaaaaa taacctgttt tatatagaac catatatccg gaagagaagg
     2461 caagaaggta ttaagacagt agagtttatc tatctgcaag aagataattt gttatttgtt
     2521 gaggcaaaac aaagtattcc aaatcaagaa agatctcctg agcgttttga tgaatatatt
     2581 tcagagattt atcaaaaatg gtgtaatgca ttaaatgttg aaattttggg aatattaggc
     2641 agagaagata tcaaagaaac aattatgcct agtgcttttt caaatttgca atggggtagc
     2701 atagaaatca aattattact ggttatcccg gatgtgccgc ttaattatct tgggcaattg
     2761 aatgaattaa taaagcaaga atttaataag aaagatactt tgcggttgat ttctttatgg
     2821 aacatttctg tagaagtaat taatagggac ttagccattc aaaaaggttt agctagctct
     2881 tgaaaatcta aagttaatta gttaattgag aacataacat gcaattctcc tactcatggc
     2941 tgaaaaccca agccgatacc gaactttcct ccgataagct ggaacatctg ttaacgatgt
     3001 ccggcttgga agtggaagag gccgaaactg ccgcgcctgc gtttgcgggc gtggtgattg
     3061 ccgaagtgaa atccgttgaa aaacatccgg atgcagaccg tttgaacgtt acccgagttg
     3121 atgcgggtac gggcgggttg gtgcagattg tgtgcggtgc gccgaatgtg aaagcgggca
     3181 tcaaagtgcc gtgttcgctg ccgggtgccg ttttgccggg taatttcaaa atcaagccga
     3241 ccaaaatgcg cggcgtggtg tcggacggga tgttgtgttc caccgacgaa ctcggtctgc
     3301 ccgacgacgg tgtgaacggc ctgcacattc tgcctgaaga tgcgcccgtc ggtaccaata
     3361 tccgcgaata cttggatttg gacgatacgc tgtttacgtt gaaaattacg cctaaccgcg
     3421 ccgactgctt gagcatcaaa ggcattgcgc gcgaagtgtc cgcattgacg gggtgcgcgt
     3481 tcaggcagcc cgaaatccat accgcgccga tcacgggcag tcgaaaacag cccgtgcaga
     3541 ttaacgcgcc tgccgattgc ggccgtttta tcagccgcgt tattgaaaat gtcaatgcga
     3601 aagcggctac tcccgattgg atgaaacaac gtttggagcg cagcggcatc cgcagtattt
     3661 ccgcgctggt ggacatcggc aattatgtga tgctggaaat cggtcagccg atgcacgttt
     3721 ttgatgccga caaactttcc ggcagcctgc acatccgccg cgcgcgcgaa ggggaaacgc
     3781 tggaatgcct gaacgagaaa accgtttccc tgtctgaaaa cacgctggtc gtggcggacg
     3841 aaaaaggcgt gttgagtttg gcgggcttaa tgggcggcgc ggcgagcgcg gtttcagacg
     3901 gcacgcaaaa tatcgtgctg gaagcggctt ggtttgcgcc cgaaatcatc gccggcaaat
     3961 cgcgccaata cggtttcggt tcggattcgt cgttccgctt cgagcgcggc gtggattacc
     4021 gtttgcaggc ggatgccatt gaacgtgcta ccgaattggt gttgcagatt tgcggtggtg
     4081 cggcaggcga gatggtggaa gcgcaaggcg aattgcctga agcgaagcag gttggattgc
     4141 gtttggaccg tctgaaaacc gtgttgggcg tggacattcc tgccgaacag gtggaaacca
     4201 ttttgcaaca cttgggcctg cagcccgaga aaacggcgga aggcttccgc gttaccgcgc
     4261 cgagcttccg ttttgacatc gaaattgagg ctgatttgat tgaagaaatc ggacgcgttt
     4321 acggctatga aaacatcccc gacgattaca cgtcaggccg tctgaaaatg ctggaactgc
     4381 ccgaaacacg ccgcccgcgt tttgccgttt acaacgaaat ggcggctcgc ggttaccgcg
     4441 aagtggtcag ctatgccttc gttgacgagc agtgggaaca agattttgcc gccaacgccg
     4501 accccatccg cctgcaaaac ccgctggcgg cgcagtatgc cgtgatgcgt tccacgctca
     4561 tcggcggctt ggtggaaatt ctgcaaaaca atctgaaccg caaacaaaac cgcgtgtgcg
     4621 tgtttgaaat cgcccgcgtg ttcagcaaag gttcagacgg ccagtttgtc caaaacgaac
     4681 gcatcggcgg attgtggtac ggcgcggtca tgccggaaca atggggcggg aaaacgcgca
     4741 atgcggattt ttacgacatc aaggcggacg tggaaaatct gttgaaaaac aaagcagtcg
     4801 agttcgttaa aaccggacat cccgccctgc atcccggacg tgccgccaat atcgtttcag
     4861 acggcaaagt catcggcttt gtcggcgaac tgcatccgaa atggctgcaa aaatacgacc
     4921 tgccgcaagc gccgctggta tttgaaatcg atatggcggc cgtgttggaa tgcgggaaaa
     4981 cgcgctatcg ggtcgtatcg aaattccagc cggtgcgccg cgatttggcg tttgtgatgc
     5041 cggaagctat gagccatgat gatttgctgc ttgtcttgaa aggcgcggca aacaagttgg
     5101 tacaggaaat cagcgtgttt gacgtgtatc gcggcacggg actgcccgaa gggatgaaga
     5161 gcgtggcggt caaagtgatt ttgcaggata tggaaaacac gctgacggat gaggcagtcg
     5221 agccgcttat cggaaaactg attggcgcgg caacggcggc aggggcgcgg cttcgcagtt
     5281 aaaaaataaa tattcgcttg aattttaaat aaaaattggt aataatccac aactgttaca
     5341 acagaaggta atcatatgac tctcactaaa gcagaactgg ccgatatttt ggtagacaaa
     5401 gtcagcaacg tcaccaaaaa cgatgccaaa gaaatcgtcg aactcttttt tgaagaaatc
     5461 cgcagcactt tggcaagcgg cgaagaaatc aaaatttccg gtttcggaaa tttccagttg
     5521 cgcgacaagc cgcaacgccc gggtcgtaat cctaaaaccg gcgaggaagt gccgattacc
     5581 gcccgccgcg tggtaacttt ccatgccagc cagaaactca aaagcatggt ggaacactac
     5641 tatgacaaac aacgttgatt tgaaggttcc cgtaaaacgc tatttcacgc tggacgagtt
     5701 gtgcggactg ttgcaaatca gcccctatgg ttttgcgcaa tggcagcatg atcacggtgt
     5761 ggttgtcggt tacggcggcg aacgctacac ccgtttggat gtggtgaaac tgttgaaatt
     5821 gcagagcacg tttgcaccgt atgcagaagg tgcggagtcg ggttcggacg gcaaccgtcc
     5881 ggttacgctt caggaaatcg gagacggtct gaaagagctg ttggcggatt tggataagga
     5941 attgtgctga tttgaggccg gttgcaggta tgcagccggt tttgttttac acgctaaaaa
     6001 ataattatag tggattaaca aaaatcagga caaggcgacg aagctgcaga cagtacaaat
     6061 agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc tatccttggc
     6121 agacggtttg cagcagattc gcatacggca gatgtttcaa gcggaaaaga aacacgataa
     6181 aaataaaaaa gcctccgaac agtcggaggc tttgctttca aactgaaatt atttcagttt
     6241 ggtttctttg tacactacgt gtttgcgggc aaccggatcg aattttttaa tttccagttt
     6301 gccgggcata gtgcgtttat ttttggtagt ggtgtagaag tgaccagtac ctgcactgga
     6361 ttccagtttg attttatcgc gcattgcagt aatccttaac taagggttta aattaagctt
     6421 caccgcgagc acgcaaatca gccaatacga catcaatgcc tactttgtcg atggtacgca
     6481 atgcagcgtt agaaacgcgc aggcgaaccc agcggttttc actttctacc caaaaacgac
     6541 gtgattgcaa gttgggcaaa aaacggcgtt tggttttgtt gttggcgtgc gatacgttgt
     6601 tgccggacat cgggcgtttg ccggtcactt tgcaaactcg tgccataatc agtctccaaa
     6661 cttctaaaat agtaaaacgc gatttatacc acgaaaaaca gtgttagttc aagtgtttta
     6721 gagaaaaacg gctgttttac gcggataatc cttccgattg tcgcatctgc ggaacgtttg
     6781 caggtgcgaa acagatgggg cgcgattata gcggatttcg ggaactgctg cattcaaaat
     6841 gccgtccgaa catatttcca gtattttcag acggcatttc aagttttcaa atccctcaat
     6901 gtccaccgcc gccgccgttg tggaacggcg gttttgccag ccatatgacg ggtatcatga
     6961 taatgaataa caagctgccc gccataaaga tttcgttcga gccgataata aaaccctgct
     7021 gggtaatggt attgttgatg atgcctaggg tttgaatgtc ggaaacgccg tgctgggaca
     7081 aatgagcggc cgtttcgtgc aatgttgcgg aatagggcgt gatgtgttcg gcaaagcgtg
     7141 tatggtgcaa cgcttcacgc cgttcccaca gggtactgac gacggatacg ccgacaccgc
     7201 ccatcagcac gcgcaggaaa ttcgacaggc tgccggcggc ggcaatctgt ccgcccttca
     7261 tgtgcgacaa ggtgatggtg gtcagcggca ggaagaacat ggcaacgcct actccctgcc
     7321 aaaactgcgg ccagatgacg ttgccgatat ccatgtcggc ataaaaatcg gtgcgccaat
     7381 agaaagtgaa ggcaaaggtc aggaagctgg cggttacgag caggcgcata tcgattttat
     7441 tgccgaacct gccgattaac ggagacagga agacgggcag gatgccgacg ggcgcggcgg
     7501 caagccccgc ccacgtggag gtatagccca ggttggtctg caacactaac ggcagcaggg
     7561 tcagcgtccc catatacacc ataaaaccca acgaggtggc aatgacgccg acggtaaaat
     7621 tccgatcttt aaacagcgat aaatcgacaa tcggatattt ttctcccaat tcccaaacaa
     7681 taaaatacga caagcacacc agtgcgacta cgcccaaggt aatgatttct ccagaggcga
     7741 accagtcgag ttccttaccc ctgtccaaca tcatctgtaa cgcgccgata ccgactacca
     7801 tcaatgtaag cccgacatag tcggtcggca tcttaacggt ttccgtttcc cgatatttca
     7861 aatgtttcca ggttatccat gccgatatga taccgatagg gatattaatg aagaaaatcc
     7921 aaccccaatg ccagtttccg gaaatccagc cgccgagtat cggcccgaga acaggggcaa
     7981 caacgacggt cattgcccac aatgccagtg ccagcgtccg ttttgcgggc ggataggatg
     8041 ccattaacag gctttgcgac aagggaatca gcggcccggc gataaagccc tgcaagatgc
     8101 ggaaaacaac cagcgactga aggttggggg caataccgca cagccacgat gtgatgacga
     8161 aaccgacagc ggcggcggta aacaatttga cctcgccgat gcgttttgcc aaaaagcccg
     8221 tcagcggcac ggaaacggcg tttgccacag aaaaggaagt gatgacccac gtcccctgag
     8281 tggttgccgc accgaggttg ccggcgatga cggggacggc gacgttggcg atagtcgtat
     8341 ctaaaacttc cataaatacg gcaagcccca aagacagcgt aacccacgcc aatgccgcac
     8401 ccttaagcgg tggataatcc attgtgtcct ccgtttcaga cggcatcagg gcgcggattg
     8461 cccgaggatt tcatcgaccg tccgatcgac ttccgaccag tcggtacttt ccatttccgg
     8521 caatgccgca cccggcgttt ttgaaacagg cgcgcctgcg gcggaagtat ccactttaac
     8581 cgtcatcgac aaaccgatac gcaacggatg cctgtccaca tcttcgcggt tcagcacgat
     8641 acggacgggg acgcgctgca ccactttaat ccagttgccc gttgcgtttt gcgccggaat
     8701 cagcgaaaac gcgctgcccg tacctgccga aaaacctgcc accctgccgc gataaacaat
     8761 ttgtttgccg tacaaatcgg acaccagctc ggcaggctgt ccgattttca tatgccgcaa
     8821 ctgcgtctct ttaaaattag catccaccca cacatccgac agcggcacca ccgccatcag
     8881 cggcgcgcct gccgccacct gctgcccgac ctgcaccgaa cgcttcgcca cctgaccgtc
     8941 cgccggcgcg cggatttgcg tccgctgaag gttcaaccac gcatctttca acctgccgat
     9001 tgccgtctga accgccggct gttcgcgcaa agaaacctga ccgcccaaag ccgcgcgtgc
     9061 cgaagattct tccgccaaag ccgctttgac cgccgcctgc gcctgagaca ctgccgcacg
     9121 ggcgtgtgcc agctcttcgg cggacaccgc gcccgattcc gccaaagcag accggcggcg
     9181 caaatcatcc tgcgtgcgtg ccaaatccgc ccggcgcaag gcaacctgcg cccccgcctg
     9241 cgaagtggcg gcattttgcc ggcggttttg ccgcaccgcc tgaaccagct cgttttttgc
     9301 ccgctcgtaa gccagcacat cattatcgtc gtccaatacc gccagcacgt cgcctttttt
     9361 cacggcatcc gtatcgtcgt gcaaaacctt ccgtaccgta ccgccctttt gcggcgtaac
     9421 ctgaaccacg cgtccggcaa cataagcgtc ttccgtttcc tcttcgtgct gccaccataa
     9481 aaaaaacgcc gacccggcgg ctgcggcggc aagcgcgaac agcagcgtca atgccgtcag
     9541 gcggcgtttg cgtttggctt gcgtgttttg aagttttgtt tcgtccgcgt gcgtatccat
     9601 aagattgggt tccgaatttc ctgatttcat ataaacaatg ttctttcaat aggaagcaga
     9661 ttctatatcc gcgcaataac gcaatcaagt cagtatttta gatgcaatat tcaaaaaaca
     9721 acgcttcagg cggcaaaact gaaaatatat ttcgtt
//
LOCUS       DAXHSJ010000065         9755 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000065 DAXHSJ010000000
VERSION     DAXHSJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9755
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.065"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(64..1206)
                     /locus_tag="ACFM8U_001137"
     CDS             complement(64..1206)
                     /locus_tag="ACFM8U_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC6334384.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQVNPAFAPISGSDNDRRIIQTALANARSA
                     GVDDIVSFSVADAQSVRPNGVGGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WQVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1255..1677)
                     /locus_tag="ACFM8U_001138"
     CDS             complement(1255..1677)
                     /locus_tag="ACFM8U_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334385.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1670..2479)
                     /locus_tag="ACFM8U_001139"
     CDS             complement(1670..2479)
                     /locus_tag="ACFM8U_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6334386.1"
                     /translation="MIQDTRPLIRVVAGILLNRDGDYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCNDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2561..2938)
                     /gene="acpS"
                     /locus_tag="ACFM8U_001140"
     CDS             complement(2561..2938)
                     /gene="acpS"
                     /locus_tag="ACFM8U_001140"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC6334387.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3138..3296)
                     /locus_tag="ACFM8U_001141"
                     /pseudo
     CDS             complement(3138..3296)
                     /locus_tag="ACFM8U_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(3319..3996)
                     /locus_tag="ACFM8U_001142"
     CDS             complement(3319..3996)
                     /locus_tag="ACFM8U_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HFC6334388.1"
                     /translation="MKLKNKYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRG
                     INPIFLKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKR
                     NGVVYTEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSN
                     EFARGKQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMK
                     MLRNNPI"
     gene            complement(4043..4657)
                     /locus_tag="ACFM8U_001143"
                     /pseudo
     CDS             complement(4043..4657)
                     /locus_tag="ACFM8U_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(4667..5446)
                     /locus_tag="ACFM8U_001144"
     CDS             complement(4667..5446)
                     /locus_tag="ACFM8U_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFC6334389.1"
                     /translation="MNQKYILSANNNSLIEEIHNTVQSIGYCIVRGLNLNHLDDSRRN
                     KKLFDFLSQLGMLTNHKDDGFKSIFLDIKYRGDDYVINNDITFSEDVGECPLHSDSSF
                     SENPESYLVMYVVKSANDGGNSLFLSSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPT
                     SFDKKQGVIWGNILSLNTQMIRFRSDCIYKGIEENRNKVSKEMVLALDYLVNVIKNAS
                     DIQEFSAQDDGLIIIDNVNGLHARTDYTDKNRHYIRARITV"
     gene            complement(5445..5552)
                     /locus_tag="ACFM8U_001145"
                     /pseudo
     CDS             complement(5445..5552)
                     /locus_tag="ACFM8U_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(5598..6326)
                     /gene="pdxJ"
                     /locus_tag="ACFM8U_001146"
     CDS             complement(5598..6326)
                     /gene="pdxJ"
                     /locus_tag="ACFM8U_001146"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC6334390.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAEFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(6379..7122)
                     /gene="recO"
                     /locus_tag="ACFM8U_001147"
     CDS             complement(6379..7122)
                     /gene="recO"
                     /locus_tag="ACFM8U_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC6334391.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVSFVPVSASWYGSQELKTLHRAEWVGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(7156..8283)
                     /gene="pheA"
                     /locus_tag="ACFM8U_001148"
     CDS             complement(7156..8283)
                     /gene="pheA"
                     /locus_tag="ACFM8U_001148"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC6334392.1"
                     /translation="MLECTINYCSGEIMSQTIDELLIPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASSSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRQDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(8320..9552)
                     /locus_tag="ACFM8U_001149"
     CDS             complement(8320..9552)
                     /locus_tag="ACFM8U_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFC6334393.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHNGSATVMAVTMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
BASE COUNT         2312 a         2576 c         2495 g         2372 t
ORIGIN      
        1 atcggccgga tattaaaaaa agccgtctga acatttttca gacggctttt ttgtacactt
       61 tcctcaacgg ttcgatccct gcaccatatc aatcaggaac aggcggcagt cgaggttgcc
      121 gttataaagc ggaattttcc gcttgggcga caggcgcatg aatttgggca tttccctgtc
      181 gccggtaaac ataccgacct gccagccggc atagtgtttt ttcaaccacg tccccaactg
      241 cggatacagt gcctgcaaag cgcggacttc ctcaaggcgc acaccgtagg gcggattgga
      301 caccataatg ccgcccacgc cgttcggacg gacggattgc gcgtcggcaa cgctgaagga
      361 aacgatgtcg tccaccccgg cactgcgtgc gttggccaat gccgtctgaa tgatgcggcg
      421 gtcgttgtcg ctgcccgaaa tcggtgcaaa agcggggttg acctgcgctt cggcgcggcg
      481 gcgcaaatcc gcccacagcg ttttatcgaa attttgcagt ttttcaaaac cgaagcggcg
      541 catcatgccc ggcgcgcggc gggcggcaat ccaagcggct tcgatagcaa tcgtgccgct
      601 gccgcaaaac gggtcttgaa acggctgcgt gccgtcgtag cctgccgaga gcagcaatcc
      661 ggcggcaagg ttttcgcgca gcggggcttc gccggtatcg agacggtagc cgcgtttgaa
      721 cagggcttcg cccgaagtgt cgatgaagat ttcgacattg cgttcgtcca aaaaggcgtg
      781 gatgcgaaca tcgggcgcgg ctttgtccac gctcggacgt gcgtcgtaaa tgtcgcggaa
      841 agcgtcgcag acggcatctt tgacggtcaa accaacaaat tggatgctct taacgttggc
      901 acgctttgcc tcgactttga ctttgaacgt ctgctgtaaa gtaaaccaat taaaccagtt
      961 gatatttttg gcgagtttgt agatgtcgcg ctcattgcgg tatgtccctt tggtcaggcg
     1021 cagcaggata cggctggcag tacgcgaatg caggttggcg gcgtaaacct gttccaatcc
     1081 gccccggcag gaaacgccgc cgtcaaacac ttgtacatcg gtacagccga ggctttcgag
     1141 ttcttgagat aaaacggtct ccaagccgcg cgggcaggtg gcgaaaagtg tatataaagt
     1201 attcataacg tgtcctttcc ggacggatgt tccggcaatg gcggattgaa aggtttattc
     1261 ggcggtaaat gccgtctgaa atgtccgact gccgattgta atgtccgccg taaaatcgcg
     1321 cctgccttcg acacagatgg gcaggcggat gcgtactgcc tgccaagtcc ccgacggttg
     1381 ccgctcgaac atatagcggt tgaaacccat atccatattt ttcatactga agctgatgct
     1441 gacctgttcc gtgccggcgg gcgcgtgttc gatataaata tcaaacggtt ttttggttga
     1501 aacggcggcg gcgcggacgc ggcttccgtc cggcagcgtg caaccctcgg tcaaatcgca
     1561 ttgcgccgcc acagcttgcg gctgctgcgc ctgccaccat tgcaacaaaa cgagcttgac
     1621 ggcggcaaag gcaatcagca gcagtgcggc aagcagtaat ttacggtttt tattcatgat
     1681 tcttccctat tgtttcatcc cttaccacca cgtgcgcccc caatccgagc catagttttc
     1741 cataacgtgc aaccgtctgt ccgtttgctg caagtacgag cggcacggat acgccctgcc
     1801 gcagggcttc tgccgcctct tgtgcctgaa catcattgca cacgcgccac acgacaacat
     1861 ccgcgccctt ttcctgcgcc tgccgccatt gttcgcgggt ctgcaccatc atccacacgc
     1921 tgtcggcgtg ttcgggtctg tcctgccatt gcgccgcctc catcaaaacg ttcgcaccgc
     1981 ttccctctgc cgaacccaaa ggcaggacgc ggtacgcgcc catactgttc tccccgtgca
     2041 aacccgtttt caggctgccg tacaaacggc gcgggacgga cagcgaacgc aaaagcgcgc
     2101 cgttggcggg cagcatgggg gcaacggtaa aatcacccgc cttctgccaa gaccattcct
     2161 gcccttcgcg ggattgcggt tcgcccgtcc attggccggg gttgacccat aggaatttca
     2221 ggcagacgcg ggcgtgttcg taggaatgga ttttggttaa ccaaggcgtg gcggcaagga
     2281 tgcggatgcc gagttcttct tcaaactcgc gttgcaaggc ttggaagtcg gtttcgcccg
     2341 cttcgacctt gccgccggca aattcccaat atccggcata gggcttgcct tcggggcggg
     2401 aactgagcag gtagtcgccg tctcggttga gcaggatgcc ggcaacgacg cggataaggg
     2461 gtcgggtgtc ttgaatcatg gcgggtacgc ggttggtcgg tcggagtgca aatgagggac
     2521 gagcaatgcc gtcaacgggt ttgccgcatt tcagacggca ttatttttcg gcaacgacaa
     2581 acgccaatac ggtgtcttct tcgtcgctca tgctgaggct gacgcggctg atgccttgtt
     2641 cctccagcca tttggacagg gcggggccgt agaaaaattc gggcttgccc aatgcgtcat
     2701 gcccgatgcc gatgttgcgg aaggaaaccg cgccgcgtat gcccgtgccg acggctttgg
     2761 cgaaggcttc tttggcggca aagcgtttgg cgaggtagtt gacgggtttg cccgcttgcg
     2821 gaaattcaag cagctcttcc ggagtgagga tgcgcccggc aaacgcctgt ccgaattttt
     2881 tgtttaagcg gatgatgcgc ttgagggaaa caatgtctgt gccgatgccg taaatcatat
     2941 ttgcgctcct tcgcccttgg ttgcgggtag tgatgaatgt gatgatgaat gccgtcaatt
     3001 tcttggggcg ttaaaatggc gtgaatatcc acttttgggg ttttgtcggt aacaattttc
     3061 actgtaacct tttgcatttg aaattctaaa acatcgtgcc aattatttaa taaggagtta
     3121 aggcatttaa taatgaatta aagtttgatt aaacccgaat gcccgatttt cagagccaat
     3181 accaataaaa caataaaaat taacatttta aaaaaatttg ggctaatctt agtccttaac
     3241 cgaattccaa catacaatcc aataacagac aatacggaca gtaaaaatat taaaccgggg
     3301 gcttaggaag caaaaatatt aaattgggtt atttcgaagc atcttcatta atattttacc
     3361 taaatcttca tgccggtggt taaacctaaa ctccgtttct ttgagatgca gataaaacat
     3421 ctctttggaa ataccatgaa atttagccaa acgcccctta gtataactcc aaaaagattc
     3481 aataccattg atatgttgct ttcctcgagc aaactcattg gaaccatgat ggacacggta
     3541 atgcttctca tagcccatat cgacaagacc gtcatatgct ttccagccgt cagtattgat
     3601 ttcactgtca gcagatatgt gaccacgtat gaccttgatt agtgaggctt tcgaagcgtc
     3661 gggaacgatt tccgtataga ccacgccatt gcgtttcagt atgccaaata cgatggtttt
     3721 acctgacgct ccgcgaccgc gcttacccct gatacgtcgc gcaccaaagt aagattcatc
     3781 taattcgacc acaccggaca gtggtgagct ttgttcgcac agagcagcaa tcctgtgtct
     3841 gattttcagg aaaataggat tgataccgcg tacactaatg ccggtcattt tagcagtatc
     3901 ggaagcggtc aaatcaagag cgaaacagcg gattatttct ctaaactttt gctctgaaat
     3961 tttgctgaac ttttgatact tattttttag tttcatatta ggagcttatc aggttttttg
     4021 atgattttgc ttcctaagcc cctaaaccgt attcactctt atttaataac caatactggt
     4081 ctcttagcat atatatttga acaattttcg ccaaaagata gcatagattg cttgatttta
     4141 cgatacgatt tttattttct gtttcgctaa gcaaaaatat taacaatatg ggagacatgg
     4201 cattggttga accgccgatg atgcctgcca aaaacccaaa aaaaaccata ttcttattat
     4261 tggcaactac ttgaatattt tttgcttttg cacatacatt taaaatacca ttgacagaat
     4321 aatacaatgt aatgattgcc atcagtaaaa gcagccaaga cactggaggt atcaaaagca
     4381 acttcacccc caaaatgctg ccaacgacgc tgccgatagc aagcaattta taggttttta
     4441 aataataaac aatctcttgc caaaaaccct ttttgttatt gctgcataga accaacaagc
     4501 tcattaacag gcttggtaat gccaccaagg caacaacctt agacaatggc atgataaaag
     4561 ccaatgcggt tgtaccgagc atcggaaatc ccatgcctgt aattccgtgc agtattgcgg
     4621 cagcaacaaa aacgatagat tgcattattt cttgcataac cgtcctttat acagtaattc
     4681 ttgctctaat ataatgcctg tttttatccg tataatcagt tctggcatgc aagccattga
     4741 cattgtcaat aataatcaaa ccatcatctt gtgcagaaaa ttcttgaata tcactcgcat
     4801 tttttataac atttacaaga taatcaagtg caagtaccat ttcctttgat actttatttc
     4861 tattttcttc aataccttta tagatacaat cacttctaaa tctaatcatt tgtgtattga
     4921 gcgataagat attaccccat atcacacctt gttttttatc aaatgatgtt ggtgttttaa
     4981 atggatataa attgcccgtt aatgttttta agtgtttttt accggtttct gttttagata
     5041 actgattgac aatatctgat gaacttaaaa atagggaatt acctccatca ttggctgatt
     5101 ttactacata cataaccaaa taactttccg ggttttcact aaaagatgaa tcactatgaa
     5161 gtggacattc tccaacatct tccgagaaag ttatatcatt atttattaca tagtcatcgc
     5221 cgcgatattt aatatccaaa aatatagatt taaaaccatc gtctttgtgg tttgtcagca
     5281 ttcctaattg agatagaaag tcaaataatt tcttgtttct ccggctgtca tcaagatggt
     5341 ttagattaag accgcgaaca atacaatacc caatactctg tactgtattg tgaatttctt
     5401 ctatcaaact attattgttt gcagataaaa tatacttttg attcatatag tgaattaaat
     5461 ttaaaccagt acggcgttgg cttgccttgc cgtactattt gtactgtctg cggcttcgcc
     5521 gccttgtcct gatttaaatt taatccacta taacatatcc ctgtcgtgaa atgctgtctg
     5581 aaagggtttg cctgccctta cggcagcagc cttgccctga acatcgcctc cttcatctgg
     5641 cgcacggctt cgggcagtcc gaggaagagg gcttgggcaa tcagcgaatg cccgatgttc
     5701 agttcgcgga tggcgaggat ttgggcgatg ggggtaacgt tgtgtatggt cagtccgtgt
     5761 ccggcgttga cgaccaagcc caaatcgccg gcaaaatgcg cgccgttttg cagacgctcg
     5821 aattgcttga gttgttcggc gtgactgcgt gcatcggcat atgcgccggt gtgcagctcg
     5881 acaacgggcg cgccgacatc ataggcggct tggatttgcc tgtcgtcggc atcgataaac
     5941 aaagacacgc gtatgcccgc gtcggtcagg attttggtga attcggcgat tttttcctgt
     6001 tgcgccaata cgtccaaacc gccttcggtc gtgatttcct gccgtttttc aggcacgatg
     6061 cacacgtctt ccggcatcac tttaagcgcg ttttcgagca tttcttccgt caacgccatt
     6121 tcaaggttca ggcgcgtgcg gatggcgttt ttgacggcaa acacgtccgc gtctttgatg
     6181 tggcggcggt cttcgcgcag gtgcatggta atcaggtcgg caccgtgcgt ttcggcaacc
     6241 agtgccgcct ccacggggct gggataagtc gtgccgcgcg cgttgcggac ggtggcgatg
     6301 tggtcgatgt tgacacccaa aagcataatg tttccttttc tcttcgtttt tctgtttggc
     6361 ggcatggtgt gggcgcagct acgcggtatt gcggtcaaac tgccttatct gttccaacac
     6421 ctgccgcgat ttcaagcctt cgggaagcag cgtgccgatc agcagccgtg tgattttcaa
     6481 tgcctgttgc aggctttcgg cggtgcggaa actgccttcg cgcaaatcga tcaggctttg
     6541 cccttcgaca attgcgccgg cctcgtgcga aagcgcgtct gcgtcctcgc atacgggcat
     6601 gaccgcctct tcgggcatca gccggtaggt tttgtccgcc aaaatatcgc cgcccgtgcc
     6661 gtcggcgtgc aaatcggggg caacgcccaa ggcattgagc agcttccact cgaagcggcg
     6721 caaatcggca atgtggttgg cttcacgaca caccgcctcc atcacttttg ccaacgcgtc
     6781 gtacagttcg ggcatcggat cttcgcgcgc ggtcagtttc aacaccagtt cgttcacata
     6841 cagaccgctg aacaacgccc tgccctgcgg ctgccgccat ccgccgaccc attcggcacg
     6901 gtgaagggtt ttgagttcct gactgccgta ccacgacgcg ctgacgggca cgaaagaaac
     6961 cagcacgccg cgcaattcgc tctgccgctt gcgcgcgctt cttgccaaaa gtgccacacg
     7021 cccgtaacgg cggctgaagg cttcgacccg caggctgctt tcgcgccatg gcgaggaagc
     7081 caacagaaag acgggttcgt ggttgactcg gtgttcggac atcatgcctg aaatgccgtc
     7141 tgaacgctgc tgccgctaca aaacggcggt cgggtacgaa ccgatgactt tgacgaacga
     7201 agcgcgttcg cccaagcgtt ccaatgccgt ctgaatctgc gcgtcctggc ggtgtccttc
     7261 gatgtcgatg aagaacaggt attcccacaa aacggatttg ctcggacggc tctcaaactt
     7321 ggtcatggaa atacccgact cggtcagcgg ttgcagcagc gaggcaaccg caccggcgcg
     7381 gttgggcgcg gaaacggcca gcgaagtctt gtcgctgctg cttgcaccgg tttcgtgatg
     7441 tcccatcacc aagaagcgcg tggtgttgtt cggttcgtct tcgatgcact cggcaaccat
     7501 atcgagtccg tagatttccg ccgccgtgcg tccggcgatg gcggcaaccg taccgtcgtc
     7561 cgattcggca accagccttg cggcttcggc attgctggac acggcaatcc gttcggcgtt
     7621 gggcaggtgt ctgcccaacc agtcgttgca ctgcgccaac gcctgcgcgt gggaaaagac
     7681 tttggcaatg ccttcggtgc tgccgttgtt tttacgcaaa aggttgtggt ggatgcgcaa
     7741 aacgacttcg ccgcacgcct gcaacgcggt aacggcaagc aaatccaacg tgcgcccgac
     7801 cgagccttcg gtcgagtttt ccacgggcgc gaccagataa tccgcctgac gggtttcaac
     7861 ctgcttgaag cagttgtcta tggtcggaca cgccatggtg tgcgcggcgt gtccgaaatg
     7921 cttgatcgcc gcctgctggg taaacgtgcc ctgcggcccc agataggcga tggtcagcgg
     7981 gcgttctacg gcgaggcact cgctcatcac ttcccgaaac aggcgtgcta ccgattcgtc
     8041 gggcagcggg cctttgttca aatcctgaat gcggcgcaac acggcgactt cgcgttcggg
     8101 acggtacact gcgcccgtgc ctttcagttc gccgatggcg tgtgcgtgtt gcgcgcgttc
     8161 gttgagcagg cgcaggattt ccgcatcaat ggtgtcgatg gcgttgcggt gcgggatgag
     8221 gagttcgtcg atagtttggg acataatttc gcctgaacag taatttatcg tgcattctaa
     8281 catcaaatgc cgtctgaagc ggtttcagac ggcatcggtt tacaagtatt cgctctgttc
     8341 gttttccttc cacgcccggt gcgaacacag ccacagaagc gcaatgccgc aagaggtgga
     8401 cgcggtcatg gttaccgcca tcacggttgc cgaaccgttg tgcaagaagg tcgccgccat
     8461 acccacgccc gcgccgatta aggactggaa cacgcccaac acggcgttcg cgctgccgcc
     8521 ctcttctttg aaataagaca taaagcatgc ctgcgtgttc gcgccgacca agccctgcgt
     8581 gccgacggaa aacatcacgc acgccaccaa gagccagaac ggaggcaacc cgaaaaacag
     8641 cacggcggcg agttgggaca ggttggcggc aaattggacg acaatccccc acagcaggat
     8701 gctttgcgga tgcgcgccgg ttttgagccg ccacgcggta atgcggttga aaaacatcat
     8761 cgtgatgatg ttgagtgcaa acgcccaagc gtattgatga ggcgtaacac ggtaaagctg
     8821 ctggtacacg aaggaagatt cggtcagaaa ggcgaacatc gaaccgaagc tgaatgcctg
     8881 aaaaaacaga taacccatcg cggcacgggt tttcaatacg cgtttgaacc gcccagccac
     8941 cagcccgaac acatcccgtc cgattttgcc gccgacggca ggcttgggca ggaaatactg
     9001 caccaaaccg agcagcacca gcgaatacgc cgccagaaaa acaaaaatcg cctgccagcc
     9061 acccaagccc tgcaacagtg cgccgaccat gggtgcgacc agcggcacaa ccatcaaaat
     9121 gatgccgata agggcaaaca tctgggcggc tttgcgtccg gaataataat cgcgcaccat
     9181 tgcgccgacg atgaccacag tcatgcccgc accgaatgcc tgcacgacgc gcaggttgag
     9241 gagctgttcg gcactcgaag caaatacgat ggcggcaacg gcaaggcagt agacggccag
     9301 tccggtcagc gcgacgggtt tgcgcccttt gatgtcggac accgaaccgc cgaccacctg
     9361 tccgaacgcc gtgccgaaca taaacaaact caggctctgt tcgatgcggt ggacatccgc
     9421 gttcagcgac tgcgccattt cgggaatcgc gggcaggtag gcatcgatgg aaaacggcat
     9481 cagcgtaacc agcatcgcca tcaaaaccgc catcagtttt tcgctcattt caggataatg
     9541 ggcagaaggc ataaattctc gtccgattcg caaacaaacc gcccggcggc ggaaaacaca
     9601 atacgcggaa acggaaaatt ttaacacacc ggcacaaaca ggcggcaaca aaagccccgg
     9661 caaaaccgga cgaaccggaa cgatatagtg gattaacaaa aaccagtaca gcgttgcctt
     9721 gccttagctc aaagagaacg attctctaag gtgct
//
LOCUS       DAXHSJ010000066         9665 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000066 DAXHSJ010000000
VERSION     DAXHSJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.066"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..349
                     /locus_tag="ACFM8U_001150"
     CDS             <1..349
                     /locus_tag="ACFM8U_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFC6334394.1"
                     /translation="LKFAAGVLSKEMQELAGMTRATVWLSDDTLVKQVDSREGQNFDD
                     SYYAFLPDMLQNPEHVIRDNRELIFTARYKGSALWAVLKYIKEVDEIYLQSYRISNDK
                     EIAKFMAKKKVLK"
     gene            459..875
                     /locus_tag="ACFM8U_001151"
     CDS             459..875
                     /locus_tag="ACFM8U_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFC6334395.1"
                     /translation="MIDVKIDNIFVVLNQIEQLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            1106..2167
                     /locus_tag="ACFM8U_001152"
     CDS             1106..2167
                     /locus_tag="ACFM8U_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFC6334396.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAEGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            2229..2555
                     /locus_tag="ACFM8U_001153"
                     /pseudo
     CDS             2229..2555
                     /locus_tag="ACFM8U_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2543..3240
                     /locus_tag="ACFM8U_001154"
                     /pseudo
     CDS             2543..3240
                     /locus_tag="ACFM8U_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            3237..3797
                     /locus_tag="ACFM8U_001155"
     CDS             3237..3797
                     /locus_tag="ACFM8U_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFC6334397.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            3808..5781
                     /locus_tag="ACFM8U_001156"
     CDS             3808..5781
                     /locus_tag="ACFM8U_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
                     /protein_id="HFC6334398.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVITGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDNTLRLHGANNRISYNNHDIFHKANKPRFAEDIESKPNTLSGY
                     GIRNFKVETFRGDLNTLKTDGIYSLSTAVGSSNLPIENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDTA
                     AVGYEDRNERQRSALVSTWTDENLNDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGARRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(6487..6789)
                     /locus_tag="ACFM8U_001157"
     CDS             complement(6487..6789)
                     /locus_tag="ACFM8U_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFC6334399.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(6761..7048)
                     /locus_tag="ACFM8U_001158"
     CDS             complement(6761..7048)
                     /locus_tag="ACFM8U_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFC6334400.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            7128..7733
                     /locus_tag="ACFM8U_001159"
     CDS             7128..7733
                     /locus_tag="ACFM8U_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334401.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            7712..8008
                     /locus_tag="ACFM8U_001160"
     CDS             7712..8008
                     /locus_tag="ACFM8U_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFC6334402.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            8005..8214
                     /locus_tag="ACFM8U_001161"
     CDS             8005..8214
                     /locus_tag="ACFM8U_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334403.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(8384..9566)
                     /locus_tag="ACFM8U_001162"
                     /pseudo
     CDS             complement(8384..9566)
                     /locus_tag="ACFM8U_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743106.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         2444 a         2717 c         2649 g         1855 t
ORIGIN      
        1 gcttaaattt gctgcgggtg tattgagcaa ggaaatgcaa gaactggcag gtatgactcg
       61 agcgacggtg tggctgtctg atgatacatt ggtcaaacag gtggacagtc gggaggggca
      121 gaatttcgat gactcctact atgctttttt gccggatatg ctgcaaaacc ctgaacatgt
      181 catccgcgac aatcgtgaat tgattttcac agctcgctat aaaggctcgg cattgtgggc
      241 agttttaaaa tatattaagg aggtggatga gatttatcta cagtcgtacc gaatcagtaa
      301 cgacaaagag attgccaaat ttatggcgaa gaagaaagta ttgaaataga cgttgggcaa
      361 ggctcgaatt cacttgcaca cgctctcgga caccctaaag ggcaggctgc ggtatcgaga
      421 ttatcaccgc ttttccaacg cctataagga atcataccat gattgatgtc aaaatagaca
      481 atatctttgt cgtcctaaac caaatcgagc agcttggcaa cgggatcgaa aaccgctacc
      541 tgctgatgcg ccgactgtcc gaaaccatgc acacggcggt caagctcaat ttccgctacg
      601 caggccgtcc gaaatggttg gggctaaaat accgcgacgg caagccgctt tcggattcgg
      661 gtcgtctgaa agacagtttt tccacactgt cagacaacga taccgccctt gtcggtacga
      721 atatcgtcta tgccgccatc cacaacttcg gcggtatggc ggggcgcaac cgcaaagttc
      781 ggattccgca acgggaattt ttgacgctga cggacgacga caaacaggct ttgatggacg
      841 atgtgcagga ttatttttcg ggtctgatac cgtgaattta taaaaccctc aaaaacgcgc
      901 tttttagcgc gtttttttat gcgggtaata caaacccctg cccaagatat aaaaatcaat
      961 cctagacgct tctaaaaagc ccctgaaaac gattaattgt gtatcgcgcg gacaggtttt
     1021 aaaaaaatgg cgggagggtt tgaagcacgc ctactctttg ttgttttttc aaataggcaa
     1081 aatgaccgta ttgagagagg tacacatgtc caaaaatgca caaaaaaccc tacttgccgt
     1141 gtgcagtttc gaggtgcagc caaaagacgg gcgaatccaa ctgctgccat atggcgaatt
     1201 tcgcgcagta gacggtcgtc cgactgatgt ccctgcgtgg tatctgaccg aagaaaacgg
     1261 tcatgatgtc gcgttgttgg ccaacagctc gcgcaatcag ttggttgtcg attatgaaca
     1321 ccagacgctc tacaaagaga aaaacggaca acctgcacct gccgccggtt ggatgcgttg
     1381 gctggagttc acgcctaaag gcatgtttgc cgaagtggag tggacggaca aggcggctgc
     1441 ggcaattgcc gcaaaagagt atcgctacat ctctgctgtg ttttcctatg acacaaaggg
     1501 atatgtaagc aaaatttttc acgcagcgct gacaaatttc cccgcgttgg acggtatgga
     1561 cgaggtgctg gcggcagcgt cggcgcaaat tttaaaaccg gaaacggagc aaaaccctat
     1621 gaaagagttg ttacagcaac tgttcggtct gcctgatgcg ggcgaagaag aactgaaggc
     1681 ggcattgtcc gcgctcgtgg aagccaagcc gaaagacgtg gcattgtctg ccgacgtgtt
     1741 cgcgcagctg gcggaaaaag acagccgcat cgcggcattg acggcgcaaa ccgccaagcc
     1801 tgatttgact aaatacgcgc ctatctcagt ggttcaagag ctgcaaagca aagtcgccgc
     1861 gctgactgcc aagcaggaag cagacaaagg caacgaattg attaccgccg cgctgacttc
     1921 aggcaaattg ctgcctgctc agaaggagtg ggcagaaggc gtattgaaac agccgggcgg
     1981 cttggcattt ttgaccggct ttattgaaaa cgcccagccg gtcgctgcac tggcaggctc
     2041 gcaaacgggc ggcaaagcac ccgacgaacg cgtcgccgca ctgactgcgg aagaggcagc
     2101 cgcagcaaaa atgctgggca tgtccggcga agaatttgta aaaatcaaag aaagcgaagg
     2161 taagtaatgg acaaatcagc gattttgacc gcaatcacgg cagcattccg caaagaattt
     2221 caaacggggg cctgcaaaac cgcctgaacc gtttaaccgc caaacaggtg cagccggtag
     2281 cgcaggcagt gcgcctgtta tccacgtcat cgctgttgtc ggtggcaacg gcattaatcg
     2341 aggcgcatgg cgaagagatg accgcgcccg atttgattga ggttaaccgc gccatgcgcc
     2401 gccgtatgca ggccgagatt gccgccttgc gggcggtgca gacggctgct gccgagtctg
     2461 gtgggctgac ggccaacgcc gtgtataccg aggcttacca aacggcagaa tccctgcgcg
     2521 cggcggcagg ccgtctgaat gcgttggttg cggcggcacg gcggaaatac cggcaatcgc
     2581 cgacgagccg ggcgcggccg ccaatgtggc gacggcgagg cgcaactgat ggccgccccc
     2641 gccggtgtgg ccaccgaatg ccgccttacc gtacaaggcg gcaccgaccg agaaagcgat
     2701 gcctcactgc tggcgcgtct gttggaaatc atccgccgac cgcccgcagg cggcaaccgt
     2761 tacgactata aaaactgggc gttgagtgtt gacggcgtaa ccagcgcata tgtttatccg
     2821 ctgcgccgcg gcttgggtac ggtggatatt gccattacct ccgccgacgg tgtgtcgtcg
     2881 gaagaaactg tgcgccgcgt acaggcttat atcgacgaga tgcgcccggt aacggcaaaa
     2941 aatgcgctgg tactcaagcc aaccgtaacg gcggtgcctg ttaccgtgca agtcaagctc
     3001 gacggcatcg acttggacga ggccaagcgc cgcatacgga cggccctaaa agaatatttc
     3061 gacaccctga tccccggcga cggcctgact gtgtcgcaaa tcgaggctgc tatcagcaat
     3121 gtggatggtg tgatcgaccg ccgtctgact gcgccgacgg ccaaccgtgc cgccgatacg
     3181 gttaaccgca tcgagtggtt taaagcgggc gcgattaatg taacggagat gccgtcatga
     3241 gctatcaaga catcttgcgg ggcctgttgc cccccgtgtc gtatgcccgc aatgccccgc
     3301 gtgtgcgggc gcaggcagaa atagacggcg cagcgctgga tgcggtggcg gaatcggctc
     3361 aaagcgttgc cgatgccgtc gacccgcgca gcgccggcca aatgctggcc gattgggagc
     3421 gcgtattagg tttggacggt acgggcaaaa accgccagcg ccgtgtgttg gccgtcatgg
     3481 ccaagctaaa cgaaacaggc ggcttgagta ttccttattt tgtgcgtttg gccgaggcgg
     3541 cgggctatca aatccaaatc gacgaaccgc agccgttccg cgccggtgtc aaccgtgcgg
     3601 gcgaccgtct tgcgccgcag gaaatcatgt gggtgtggca cgttaacgtg cgcggcggca
     3661 acaaccgcat tacccgattc cgcgccggta tctcggcggc gggcgacagg ctgaccgatt
     3721 acagcgatgc cgtgatcgaa agcctgttca accgcctcaa gcccgcccac accgctatcc
     3781 gatttaccta ccgataagga ctaaaccatg caccccatcg aaacccccga caagaccttc
     3841 cacgacggcg acggcgtgtc cgaattgggc accatcctgc ccgcgtggtg gctcaaccaa
     3901 gtgcaatccg agctgctggc cgtgctgact gcggccggta tccagccgga taagtcgcag
     3961 cctaatcaat tactggcagc actaaatagg ctggctgtag ttatcaccgg cgaccaaacc
     4021 attaccggcc aaaaaacctt tactgcccaa acccaattcc aaagcggcat ccatttatcc
     4081 gccaaccaga cgaactggaa cggcggccac aaagcctaca tcggcgcgga tgccgacaac
     4141 gcccacatcg tcttcggcga caacaccctg cgcctgcacg gcgcaaacaa ccgcatttcc
     4201 tacaacaacc acgacatctt ccacaaagcc aacaaaccgc gttttgccga agacatcgaa
     4261 agcaagccga acacactgtc cggatacggc atccgcaatt tcaaagtcga aaccttccgg
     4321 ggcgatttga acaccctcaa aacagacggc atctattccc tgtcgacggc ggtcggcagc
     4381 tccaacctgc ccattgaaaa caccgcctgc catatccaag tcatcgccgg cacgaaacac
     4441 ggctggtgca ggcagttggg ttatcccgcc tacacgtccg acgtgtacga acgccaccaa
     4501 acgagcagcg caaacgacaa ctggtccgca tggaaaaaac tcaattcgga cggcatcccc
     4561 gtcggcgcga tcgtatcctt tcccaaagcc gtccgaaacc ccgcaggcta tctcaaagcc
     4621 aacggcacga cctttgcaca aaacaccttc cccgacctct accgcgccct gggcaacagc
     4681 aaccgcctgc ccgatttaag ccgtaccgac atcggcatca ccgcgtggtt tccgtccgac
     4741 caaatcccga ccggctggct ggcgtttgac gacatccgca cgcgcgtaac cgaaaccgct
     4801 tatcccgagc tgtaccgtct gctgaccgga aaatacggca gcatccaaaa cgtcccgcag
     4861 gcggaagacc gctttatccg caacgcgggc aacagcttgg cagtcggaac gaagcaggaa
     4921 gacgaaatca aacggcacgt ccacaaagta ttttcacact ggacaaacca cacagacacg
     4981 gcagccgtcg gttacgaaga ccgcaacgaa aggcagagaa gcgcgctcgt atcgacttgg
     5041 acggacgaaa atttaaacga caacggcttt ttaaccccgc gcccggacag caaaatggcg
     5101 acaggcggcg acgaaaaccg ccccaaagcc ttggttttaa aactgtgcat caaagccgca
     5161 gacaccttgg gcgaagccgt gttctggata aagtcccacg gcgaaaccat caacgcaggc
     5221 gcgctggacg cgggcacgct ggcgcaaggt ttgcaagaca aagccgaccg cgaccacacc
     5281 cacaccgccg cccaaatcca agggctggac gaaaaaatca gcaccgccgt tgccgcgcaa
     5341 ttcacacgcc aaaccatcgg cggcgtggat attgtcagat tccccgacgg cacaatgata
     5401 cagaccggca gctacaggtt cacacgaagc ggcggcccca tcgaaaacga agtcgtcttc
     5461 cccgtcgcct ttgccgacgg caacgtcaaa tgcttcgtat ccgaacgcca ttcgggacgc
     5521 gttaccggcg ctcgaaggca acacaactgg ctgtttatcc gcgcaaaaaa ccacgccgcc
     5581 gccattatta ccaactggta cgaaggcagt tgcgactgga tggccatcgg caaagccgcc
     5641 tcgggaaacg ccgccagctc cccgataggc cccgaaatac ctgaaaccaa cgaagaaccg
     5701 caaagagaga gtggaagaac atcaaccgga ccccgaaacc gccgccgccg agacggcttg
     5761 ctcgaggcac tgcaagacta gcgggctgta gagatggctg tagagatggc tgtagagatg
     5821 gctgtagaga tggctgtaga gacgggctgt agagatggct gtagagatgg ctgtagagac
     5881 gggctgtaga gatgggctgt agagatgggc tgtagagatg ggctgtagag atgggcttca
     5941 gcccgccgat ccaagcaatc cgaccgaaac cggccgcgcc gccaatcccc cgaaacctat
     6001 gccccgccaa tcctgccact cttcgtcatt cccgccgctt tcgtcattcc cgcgaaagcg
     6061 ggaatccagc ccccaaacgc ggcaggaatc tatcggaaaa aacagaaacc cccgccaccg
     6121 tcattcccgc gcaggcggga atccagaccc cgacgcaaca ggaatctatc ggaaaaaaac
     6181 agaaaccccc gccgccgtca ttcccgcgaa agcgggaatc cagaccccaa acgcggcagg
     6241 aatctatcgg aaacggctga aaccgaacgg actggattcc cgcctgcgcg ggaatgacgg
     6301 cggcaggggt ttcgggattc ccgccttcgc gggaatgacg gaaagtggcg ggaataacga
     6361 aaggcgggaa tgaccgcgca aaaagccgct gcccccttcg gacggcaccg gcaacaaaaa
     6421 accgcacggc cgaaaccgcg cgggaaagga tagtcgggcg cgcccgataa gcagcggccg
     6481 cccccgttat ttcaattggg cgacatattg gcgcaaaacc tcgttgatgc gcgtctgcca
     6541 gcccttgccg ccggcgcgga atttttcgac cacatcggcg gacaggcgta tggtaacgag
     6601 ttgtttcggg gttttgcctg tgtttcgttt ttgcattacg cccttttctt ccaattgttt
     6661 ttgatgggaa aaaagcacct gtgccaagtc ttcgggcagg gcttcggcaa tggggcgggc
     6721 aagcgcaaaa tcttcggcgg caagttcccg cacttcgccg tcagcgtttg ttaaggattg
     6781 acgttgcata ttttttaacc tctcttttat tcgctttgcg aaaactgatg acacggatgc
     6841 cgtcttttat cggcgtaaaa cagacaatgt gcaggcgttg cgtatcgcct agataagcag
     6901 cggcaacata acgcggttcg gggtaatcaa agcggacatc gggcacaata acggccgttg
     6961 tccagcgtat ttgcccgact gattcaaagg gcaaattccg ctcttcgata ttgcgttgat
     7021 ttttttcgga gtcaaattca atcttcattg cagcttgcag cgtattttgt cgttacatta
     7081 taagcggcaa aaaaccaaaa tgtaaataca aaaaaggaaa ccccgaaatg accatctatt
     7141 tcaaaaacgg cttttacgac gacaccttgg gtagcatccc cgaaggcgcg gttgccgtcc
     7201 gcgccgaaga atacgccgcc cttttggcag gacaggcgca gggcgggcag attgccgcag
     7261 attccgacgg ccgccccgtt ttaaccccgc cgcgcccgtc cgaataccac gaatgggacg
     7321 gcaagaaatg ggaaatcggc gaagccgctg ccgccgcccg tttcgccgaa caaaaaaccg
     7381 ccacggcatt ccgcctcgcg gcaaaggcgg acgaactcaa aaacagcctc ttggcgggct
     7441 atccccaagt ggaaatcgac agcttttaca ggcaggaaaa agaagccctc gcgcggcagg
     7501 cggacaacaa cgccccgacc ccgatgctgg cgcaaatcgc cgccgcaagg ggcgtggaat
     7561 tggacgtttt gattgaaaaa gttgtcgaaa aatccgcccg cctggccgtt gccgccggcg
     7621 cgattatcgg aaagcggcag cagctcgaag acaaattgaa caccatcgaa accgcgccag
     7681 gattggacgc gctggaaaag gaaatcgaag aatggacgct aaacatcggc tgaaaaaata
     7741 cgtttaccac ctgttggtag ccatcgacca actgttcaac gccctaaccg gcggcgcggc
     7801 ggacgaaacc ctctcaagcc gcacctatcg cggcgcgcgg ctcgcccaaa agcccaaaac
     7861 ccgctggaag gttttataca ccctgatcaa cggcgtattt ttcgaccgcc aacactgccg
     7921 gcaggcgtat atcagcgaac tgaaaggcag gcagcacgac gcgcggttca accaaagccg
     7981 cgccgccggg gaaaagggga cgcgatgaac tacttcggca tggtagagtt tctgcgcctg
     8041 atggcaatgg tgcggccgcc atttgtcttc ttctccggca cccgcagcga actgcctgct
     8101 tatcttgacc tcgtggcaga actgcgcctg accggatggg agcgttttgc aggcagccaa
     8161 accttgactg tgagcagcac atcaacagca attcaagcta cgacgaccac ctgatttata
     8221 agttctgacc gcaagtagcg tactactttt aaaggcataa gataatcccc gtttaacaga
     8281 ccattaaacg gggataaatt tgtgcaaaag ctaatacaat ttcctacgct tcggcggtgc
     8341 aaaagctgcc gccaattcgt gcaaaagctg ccgccgcctt acattccagt gcaatgccgt
     8401 ctgaaacttc gctaatctcg ggttgccgcg cgctgtgttg ttcttcggta ctcagcaaaa
     8461 agccgtacag acgctccaat cgggcgcggt aggcggtgaa tttgttgtag aacatccgga
     8521 agaaagaaag cgcgttttgc agtcgcgcga aggcttggac ggtctgctgg atgtcgccga
     8581 ttttgatttg tccggcaaac aggcgcggcg cttgcaaaat aatggggaag agcttgatgc
     8641 cgttggtgaa catatcatta aagccgctca ggcagacgct ttgtcgcgca atacgccagc
     8701 ggttgcggat aatggcttta aaacggtcag aaagctggtc gtgttcgtgt ttttcgccgc
     8761 tgtaaaacgc cacgctttcg gcgtggtcgc gcacgcggat aagggaataa cggtagtcgc
     8821 cgttgagttt ttcgttttca tagttgtgac gaatcaatgg gttgcctatc cacatggcga
     8881 taaaggtcgc caaaatcaca aatatataga caaaccaaac gatgccgtgc ggaatgtcga
     8941 agccgaacac ggtcaggatg ccagccaagc cccgcaaaac aacggcaaat tccagagaag
     9001 taacgaccga attgaccatg ccgcgcacaa attcgatggt cgaagcgata aattcctgcg
     9061 cgtcctgttg gatacgctgg tcgatgttgt ccggcgcgtg gcggcgcatt tgcaggcggt
     9121 agtagttttt gtcggcaagc cagcgtgctg tcagcacttc gttgagccgc tccgaccatt
     9181 taatcgccaa gccttgatcg aggaagtcgt tgacgacgtt gttaaacgcc cgtatcagca
     9241 ccacgcccgc gttcattgcg gcaaacatcc aaaatgccga agcatttcaa atcctgcatc
     9301 gagtcgtaaa gtcctttgga cataaaggta ctcaacacat tcagccgtac ttcggtcaac
     9361 agcagcgtaa tcatcgccgt aatcagcagc aagactttga ccgcgctttt cggagtcagg
     9421 caaaggcgca ggatatggct gaatgcgcgc ccgaagcgcg ttttgcgcac gacaaacaga
     9481 atcaccgctg aagcggcggc gaccgtcaat aaggtctgca acagccaaga cggcgtggaa
     9541 taaagctcgg tttgccattt ctgcatggga aatttcttac ggtatcaatg ccgtctgaaa
     9601 aagacgggta cagttgattt tttgatgaag tttggggaag ttttgccggt cagggtacat
     9661 tgcgt
//
LOCUS       DAXHSJ010000067         9644 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000067 DAXHSJ010000000
VERSION     DAXHSJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.067"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(18..302)
                     /locus_tag="ACFM8U_001163"
     CDS             complement(18..302)
                     /locus_tag="ACFM8U_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334404.1"
                     /translation="MPSEPPFRRHFTHQNRKTAPKIIITKHNGKPDTKPNTAAKMPSE
                     AVGATILIWGPLQGRITFLNHPFDSQINSAFACQKQNQYFAVRIGTFIVD"
     gene            complement(308..1363)
                     /locus_tag="ACFM8U_001164"
     CDS             complement(308..1363)
                     /locus_tag="ACFM8U_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6334405.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(1469..1951)
                     /locus_tag="ACFM8U_001165"
     CDS             complement(1469..1951)
                     /locus_tag="ACFM8U_001165"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6334406.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(1998..3134)
                     /locus_tag="ACFM8U_001166"
     CDS             complement(1998..3134)
                     /locus_tag="ACFM8U_001166"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC6334407.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKSWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            complement(3131..3676)
                     /locus_tag="ACFM8U_001167"
     CDS             complement(3131..3676)
                     /locus_tag="ACFM8U_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFC6334408.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKNREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTHQTGIRANT
                     QTDFSKFKGCLKDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(3673..5148)
                     /gene="trpE"
                     /locus_tag="ACFM8U_001168"
     CDS             complement(3673..5148)
                     /gene="trpE"
                     /locus_tag="ACFM8U_001168"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC6334409.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            complement(5259..5624)
                     /locus_tag="ACFM8U_001169"
     CDS             complement(5259..5624)
                     /locus_tag="ACFM8U_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334410.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            complement(5645..7555)
                     /locus_tag="ACFM8U_001170"
     CDS             complement(5645..7555)
                     /locus_tag="ACFM8U_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6334411.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDRGEKIGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKEATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFTYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     TLNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(7574..8218)
                     /locus_tag="ACFM8U_001171"
     CDS             complement(7574..8218)
                     /locus_tag="ACFM8U_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6334412.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(8382..9200)
                     /gene="opc"
                     /locus_tag="ACFM8U_001172"
                     /gene_synonym="opcA"
     CDS             complement(8382..9200)
                     /gene="opc"
                     /locus_tag="ACFM8U_001172"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HFC6334413.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT         2094 a         2099 c         2738 g         2713 t
ORIGIN      
        1 cgctgtactg gtttttgtta atccactata aatgttccga tacgaactgc aaaatattgg
       61 ttttgtttct gacaggcaaa agcactgttt atttggctgt caaaaggatg gttaaggaaa
      121 gttatgcgcc cctgaagcgg gccccagata aggatggttg cgccgacggc ttcagacggc
      181 attttggcgg cggtgttggg ttttgtatcc ggtttgccgt tgtgttttgt gatgatgatt
      241 ttgggcgcgg tttttctgtt ttgatgtgtg aaatgccgtc tgaaaggcgg ttcagacggc
      301 atagcggtca tttttgtgcg gtcaggcggt cgaatatgcc gccgtcggcg aagtaggttt
      361 tcatgatgtt gtcccatccg ccgaattttt cttcgggaga gaatgagtct aagtctggga
      421 agtcggcttt gtgtcttgcc aatacttcgg ggttgcgggg gcgcaggtag agtgaggcgg
      481 cgagttcttg cgccggttcg ctccaaaggt attcgagata ggcgcgggcg gttttttgcg
      541 tgcctttttt cgcgacgacg ctgttgacga cggcgacggg gctttcggcg gaaatggtgt
      601 agctcggata gacgatttca aactgtcctt gggtcagttt tttgctgacg tagttggctt
      661 cgttttcaaa agtaatgagt acgtcgccga tgttgcgttg tgtgaaggtg gtggtggcgg
      721 cgcgtccgcc gttttcaaaa acgggggtgt ttttgaggat ggatgcgacg agtttttggg
      781 cttcctgttc gttgccgttg gtggttttca gaccgtaacc gtatgcgccg aggaaggcgt
      841 agcgtccgtt gcccgaggtt ttgggattgg cgatgacgat gttaacgccg tctttggcaa
      901 ggtcgttcca atcgcggatc tgtttggggt tgttttttcg gacaaggaaa accatagtgc
      961 tggtgtaggg cgcggcgtgg tcggggaggg cttgttgcca gcctttttct accagtcctt
     1021 ttttttcgag caggtcgatg tcggaggatt ggttcatggt tacgacatcg gcttgaaggc
     1081 cgttggctac ggataatgcc tgtttgctgg agccgccgtg ggactgttgg atgctgacgg
     1141 atgtgccggg gtgttcggat tggtatgttt tgataaataa ggggttgtat tctttgtaaa
     1201 aatcccgtgc cacatcgtat gaggcgttga gcagggtaat gttttttccg tcggattcgg
     1261 tattggccgg ggcattttgt ccggacggat ggtttgaatc ggctgcgggg ctgcaggcgg
     1321 tgagcagggc tgcggtatag agtgccggtg cgtaggtttt catatgcttg tcctgtcggt
     1381 tggtagatgg ggcaacttta tacggctgtc tgcgcttgtg gaaataatgt ttgatttgaa
     1441 gattatcagt tttggttata aggacggatc agaggtgttt ccgcatcagt tcgcatttga
     1501 ttttgatgct ggggtcaagc tgcaatactg ccgaaccgag cgattcgtag cggttgagaa
     1561 ggtaaaacgg gactgagttg agcgatgcgt agagtttcag aaagctcaaa ccggatttgt
     1621 gggcgatggt ttccgcctga tgaagtaggg cagtgcccag tccgaggttg tggaacaggg
     1681 ggtggacgta aagtgcatcg agttgtgctt cttggcagtc gatttggaaa aatccctgta
     1741 tgttgccttt gtattcggca acccaaagtg ctttgtcggg atcggaaatg gtcggcaggt
     1801 agctttctgt gtttaacaag ccttcccata cttttagggc gtgttcgttg tagctgagga
     1861 tgcaggtgta ttggacggag tgcaggtgga ctttgaagat gtctttgcag tcttgcacgg
     1921 tggcggggcg taacagtgtc agcaggttca tggcagtatg tcggcggctt cagacggcat
     1981 ctgtgccgtt ggtcggatta tagggactga tgcagttttt ttgcttcttg aaatgcggtg
     2041 tccgaatcgg tggttaaaac ggtaaagtgt cccattttcc gccctttgtg cgcggttttt
     2101 ttgccgtaaa ggtgcaggtg cgcgtccggc cggctttgca ggggaaacca atccggttcg
     2161 ccgccgtctt cctgccaaac gtcgcccaaa atattcgcca tacagcaagg cgataataat
     2221 ttggtgtcgg caggcggcag gttgcacata atgcgtacct gttgctggaa ttggtctgcc
     2281 gcgcaggcat cgacggtatg gtggccggag ttgtgcggac gcggcgcgat ttcgttgacg
     2341 accaattcat gtgtgtcacc gacaacaaac atttctaccg ccaatacgcc gacataatcc
     2401 aattcgtccg ccaagcgttg cgccatctgc cgcgcctgtt gctgcacgtc ggcactcagt
     2461 cgcgcgggga cgatggaata agccaagatg ccgttttcgt ggatgttttc ggcaggatcg
     2521 aaagtttgca cgttgtcatt gttcagacgg catacgataa cggaaatttc gccgcgcaag
     2581 tccaccattt tttccaaaac gcaatccacg ccgcggtgtt cggcaaacgc ggctttgagt
     2641 tcatccaccg tttttacgcg gatttggcct ttgccgtcat agcccaatgt agcggttttc
     2701 aggatgccgg gcagaaattg tatgctttct tcagtgatgt cttcggcttt gcaaattgct
     2761 tgatacggcg cggtttgcag gcctgctttg cgtatccatg acttttcctg aatgcggttt
     2821 tgcgcgatgg caacgcagtc gccgctgggg gaaacattgg tatgtttggc gagaaaacgc
     2881 atcgcatcgg cattgacgtt ttcgaactcg gtcgtaacag ccgcacattt tgccaattct
     2941 tccaaagcgg tttggttgtc aaacggcgca cacaaatggc ggtcggcaaa ttccgctgcc
     3001 ggcgcattcg ggttgggatc gagtacggtt actttgtagc ccatggtttt ggcagcaaca
     3061 gtaaacattc tgcctaattg tccgccgcca agaatgccga gcatggcggg cggagaaaga
     3121 gatatgtttt tcatgctgac tcttcaaatt gtacaagttg atagctataa ctaattcttg
     3181 acggatgtct tgtatcgctg gaattaccag tttcagaaat acagaatact ttttccataa
     3241 atttttctgc ttttagaaat tccagtattc tgtttttttc atccttataa gcaccgcggt
     3301 ctgtacccca tgcaagaata atcatatcgg catctttcaa acatccttta aatttggaaa
     3361 aatcggtttg ggtgtttgcc ctaattcctg tttgatgtgt agagtaactg gaaaaaatat
     3421 ttaacatttt gaagttggta aaaccgtaca tatccaagaa acgtgcaagt tgggtaaggg
     3481 ttttgtcgct tctttcatca tttgctttac ttggattaat tcctatagcg acagctgaga
     3541 aatttttagg attttctgtg ttgccgctcc atctaaccgt taggatttct cgattttttt
     3601 cattatctgt atagagaccg tctttggtag tcggtctgag tgtttggcga agctcataaa
     3661 gtttttcata agtcatttat ccaacccttc ctgcaccatc tgcgcggcgc gtataacggc
     3721 tcgggctttg ttttgtgttt cctgccattc ggaggccgga tcggagtcgg caaccacgcc
     3781 cgcgccgctt tggacgtata gcgtgttgtt ttttactacg gcggtgcgga tggcgattgc
     3841 caaatccatg tcgttgttga aaccccatac gccgacggca ccgccgtaga tgccgcgttt
     3901 gctcggttcg acttcttcga tgatttccat ggcgcggact ttgggtgcgc cggagagtgt
     3961 gccggcaggg aaggtagcgg cgaggatgtc catgttggtc atgccgtctt tcagacggcc
     4021 ttcgacgttg gaaacgatgt gcattacatg ggagtatttt tcaatcacca ttttgtcggt
     4081 aactttgact tcgccggttt tactgatgcg gccgacgtcg ttgcgtccta agtcaatcaa
     4141 catgacgtgt tcggcgattt ctttggcatc gcttaacaaa tcttgttcgt tggcaaggtc
     4201 ttcggcgggg gttttgccgc gcaggcgcgt gccggcgatg gggcggacga tgacatcgtc
     4261 gcgttcgcgg cggacgagga tttcgggcga ggagccgacg atgtggaaat cgccgaaatc
     4321 gtagtaaaag agataaggcg aagggttgag cgtacgcagg gcgcggtaga gggcgagcgg
     4381 gctgtcggtg aattccatgc tcatgcgctg gctggggaca acctgcatgc agtcgcctgc
     4441 gaagatgtag tctttgattt tgttaacgca ggctttgaac ggctcttcgc cgaattcgct
     4501 gacggcttcg gtgtgtttgc tgccgagcga gagcgggatg gcgcagcttt ggcgcaactg
     4561 ggtgcggatg tcttcgaggc gttcgcgggc gcgttcgtag ccgtcgggct gcgacggatc
     4621 ggcgtaaacg acgagatgga ttttgccgct caaattgtcg atcaccgcca attcttgcga
     4681 cagcatcagc aagatgtcgg gcgtgccgag cgggtcggct ttggtggtgt ttttcaggcg
     4741 gtgggcgaag tgttcaaaat tgtagatggt ttcgtaaccg aagtagccga ccagtccgcc
     4801 ggtaaagcgc ggcaggcttg ggatttcggg tgttttgaag cggttgtgga aggcttcgat
     4861 aaagggcagg gggttgccgt cgtgttgctc gacaatttcg ccgttttgat aaacatcgac
     4921 gtgtttgccg ctggctttga gatagtggct gcaaggcagg ccgataaaag aatagcggcc
     4981 gaaacgttcg ccaccgacaa cggattcgag caggtaggta taggggcggt tggcgagttt
     5041 gagatagagg gaaagcggcg tatccaaatc ggcaaggagt tcttgcacga gcgggatgcg
     5101 gttgtagcct tgggcggctt gggcttggta ttcttgtttg ctgatcattt ctgctttccc
     5161 aaagggcggt ttcggacggc gcggcaacgg gcgcgagtat agcattttat cggaattgtt
     5221 gacagtctga ccggagatgc cgtctgaaaa ggacgggctt acggctttgt tttgcgctgc
     5281 cggtattgtt tccatttaaa cagataacct gcggttacgg gaatcagggc taagatggtt
     5341 ttgaacgtcc atgatgcata ccaaggtaaa gaaccgggtg cgaccgattg attcagatac
     5401 ggccaccaac aggcagtcag gcagccgagc agcagcggcc agatatgccg gctgaggttt
     5461 ttttgccaaa ggaggaatga tgcggcagac caaagcaggg taagggcagt gatttttaac
     5521 acggtataag catccgctcc aaacagggcg gcgagaaaat acgtaccggc aatccagagg
     5581 acggcaagta tcaggacaat gatttcttca ggtcgtttga acattttttt cttcctgttt
     5641 gatttcagac ggcattgccg ttctgtttgg tttccagcag ctcccagcgt tccagctttt
     5701 ccaaaagcag catttcgatt tcttcggcgc ggctttgcag tgcgccggct ttttcgtaat
     5761 ctttgaagat ttcgggatcg gaaagctgcg cgttgatttc tgcctgttcg gcttccaagg
     5821 cggcgatttc gtcgggcagg gcgtcgagtt cgcgctgttc tttgtaggac agtttgaccg
     5881 tgcggttggc tttgggtttt tctttcaccg gttcggcatc ggctgttttg ggtgcggatt
     5941 ccgtctgaat cttcgcttcg cgtgattttg cgtcgatgta gtcctgatag ccgccgatat
     6001 attcttttaa ccttccattt ccttcaaaaa taatgctttg ggtaatcacg ttatcgagga
     6061 acatacggtc gtgtgagaca aggaataccg tgccttgata atcgcgcagc aaatcttcaa
     6121 gcagctcttg ggtatcgata tccaagtcgt tggtcggttc gtccaaaatc aaaatattag
     6181 caggacgggt aaagagtttt gccagcaaaa ggcggttgcg ttcgccgccg gagagcgatg
     6241 aaacgggact ttgtgcgcgg gctggatgga ataagaaatc ttccagatag ctcattacgt
     6301 gttttttctt gccgccgact tcgacgtaat cgttgccttg accgagcgtg taaaacacgg
     6361 tgtcgttttc gttcaacgtg ctgcggaatt ggtcgaaata ggcaacttcc tgcttactgc
     6421 cgatacggat tctgccgtag gtcggctgca attcgcccaa aatcagcttg aggaaggtgg
     6481 ttttgccgat accgtttggg ccgattaagc cgattttgtc gccacgctgc aagatggcgg
     6541 agaatttgtc catgatgact ttgtcgccat aggtaaacga ggcatgttcc agttcggcaa
     6601 tgattttgcc gcttttctcg ccgctgtcga gcttgaaatt gacctgccct tgtacgttgc
     6661 ggcgttcggc acgctggcgg cgcagttctt ccaaacggcg cacgcggcct tcgttgcggg
     6721 tacggcgcgc ttcgatgcct ttgcgtatcc acgcttcttc ctgcgcgtgg aatttgtcga
     6781 agaggcggtt gtgttccgct tcgactgcca actcttgcgc ttttttctcg ctatatttgg
     6841 agaacgagcc gggataggaa cgcaaaatac cgcgatcgag ttcgacaatc cgcgtggcga
     6901 tattgtccaa aaaacggcgg tcgtgggtaa tcacaaccag gctgccttca aacgctttga
     6961 gcaggttttc caaccaaata atcgcgtcga tgtccaaatg gttggtcggt tcatcgagca
     7021 gcaatacgtc gggcttctgc acccaagcct gcgccaaggc gacgcgcttt ttctgaccgc
     7081 cggagaggtt gccgattttt tcgttttccg gcaaaccgag ttcgcccaaa gtctgcttca
     7141 ccgccgcatc caacttccag ccgtccttcg cttcgatctc aagttgcaat tcgttgagct
     7201 ctttcaataa ggcctcactc gaaccatttt ccaactcatg gctgacatga tgataacggc
     7261 gcaataaatc gcgaatttcg cccaaacctt cggcaacagt atcaaatacg gttgcttcct
     7321 tatcaaaaaa ggattcctgc ggtacataaa cgattttgag gttgttttgg acaataatct
     7381 gcccatcatc aagcttttgc acgccggcga ggatttttaa aaacgaagac ttacccgcac
     7441 cattacgacc gattaagccg attttttcgc ctctgtcgag ttggaaggaa gttttgtcga
     7501 gcagggcgac gtggccgacg gcaaaggaag cgttttctac agataatata ttcatgatac
     7561 aaattctcaa cagttaccgt ttggatttta ccgcaagttt ggcgcgggca atttcaaccg
     7621 cacccggcag gacggaaaca ataatgatgc cgcccatcac caagcccaga ttgtttttta
     7681 cgacggggaa gttggcaaag aaatagcccg cgtaagaaaa caggataacc cacaacaagc
     7741 caccgatgat gttgtagcgg ataaatttgg catagtgcat tttccccata ccggcgacga
     7801 agggggcgaa ggtgcggacg atgggcataa aacgggcaat gatgatggtt ttgccgccgt
     7861 gtttttcgta aaaacggtgg gttttatcga gatattcacg tcggaagatt ttagaatcgg
     7921 gattggcgaa cagcctgccg ccgaaatatt tgccgacggt aaaattgagc gcgtcgccga
     7981 gtatggcggc aaggcttaat aatgcaacca tcaaatgaat atccataccg cccagcgcgg
     8041 caataccgcc ggcggcaaac agcagcgaat cgccgggcag taagggcgta acaatcaggc
     8101 cggtttcgca aaaaacaatc aaaaacagaa tcgcataaat ccacacaccg tattgcgccg
     8161 acagcgcgag caggtgttgg tcgatatgga ggatgaagtc gatggcggag gcaagcacgg
     8221 cgcgttccta aaaaaaacaa accgcgtatt ttaaccgatt ggaaaaatgc cgtctgaaat
     8281 tttcagacgg catcggctat tcaaattcat ttcacgtaaa aaccgcaaac caaaatagtt
     8341 tgcggtttgg catttaaagt gacaatgatg atttcaaatc atcagaattt tatgccgacg
     8401 cgcaagccgt attcacgaat actggttttc gggatggtga gcgatacgtc gccactcttg
     8461 gttgttacac taaactcgcc ggattctttg taagtgcgtt gtttgtagaa cggccccgcc
     8521 tcgatgctgg cggattcgcc cagtttttta ccaatatttg cacccaatcc aaagccccag
     8581 ccgttggttt tttgattgat gtcttttttg agattggtaa cgccggtgtt taatttatag
     8641 cgggaattga ggtcaaattt cacttcagac caagggttga tataccagcc gttacccagt
     8701 tgggaaagca aatccgcgtg aactttggct aaccacgact gacggctgct gtgaagcgta
     8761 tgcttggtgg ttttaatgct gtcttttgaa gattcaaaac ccaagccggc acccacacgg
     8821 aaatttaaag aatcacttaa cgtttgggtg taggtgtagc ctgtgtaaag atcgatacgg
     8881 ttttcaggaa cgccggtggg cagttttaca tttttagtct tacccagctt gttctcatct
     8941 gtttccaaat taataatatt ttttttgctg cgcccgaaac cggcttccaa gcggatgcct
     9001 tggttggcat caaaaggaat atcagcacgt acgccgatgt gtttggcagc tttgtgtttt
     9061 tctttcagga aagcacgagt tgaagaaatg gaagagaggt cggtgtggac ggtaaactca
     9121 ttagcggttt gaagctcttg tgcagcggcg gcagtaccgg tcagggcaat catggcacat
     9181 gtaaaaactg tttttttcat agttaaaatc tccaaaattt ggattgtagt cggatatggt
     9241 aacataacgt aaataatcgt tacgcttaca attatattct taagctttcg ggggggggga
     9301 ttttacatat attaataaaa attaacaaat agttatttgt ttacaacgaa ttgttattct
     9361 cacttggttt tctgtttttt atgggaatga cgaaatttta ggtttctgat ttcggttttc
     9421 tgttttaagg gaatgacgag acttgagatg gcggcattta tcgggagcaa ctgaaaccac
     9481 tctgccgtca ttcccgcgaa agcgggaatc taggttcgtc cggtttcggt tatttccgat
     9541 agattcctgc cgcgttgggg gtctggattc ccgcctgcgc gggaatgacg aatttcaggt
     9601 tgctgttttt ggttttctgt ttttgtgaaa ataacaggat gaga
//
LOCUS       DAXHSJ010000068         9633 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000068 DAXHSJ010000000
VERSION     DAXHSJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.068"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(44..3076)
                     /locus_tag="ACFM8U_001173"
     CDS             complement(44..3076)
                     /locus_tag="ACFM8U_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6334414.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPSGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFEDVPSERPSRRASDTEADE
                     GAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3241..3921
                     /locus_tag="ACFM8U_001174"
     CDS             3241..3921
                     /locus_tag="ACFM8U_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFC6334415.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLSYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3950..4309
                     /locus_tag="ACFM8U_001175"
     CDS             3950..4309
                     /locus_tag="ACFM8U_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFC6334416.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            complement(4441..4860)
                     /locus_tag="ACFM8U_001176"
     CDS             complement(4441..4860)
                     /locus_tag="ACFM8U_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334417.1"
                     /translation="MISIFKAYYDVYEFNGELLKRCIFLDEENNEIIKDRIYEYLIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYNNEFDEEDGW
                     PDLSCSLHTFKTTLIAWNAFLQLPKSIHSVVETVIEE"
     gene            complement(5350..5886)
                     /locus_tag="ACFM8U_001177"
     CDS             complement(5350..5886)
                     /locus_tag="ACFM8U_001177"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC6334418.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5920..6276)
                     /gene="folB"
                     /locus_tag="ACFM8U_001178"
     CDS             complement(5920..6276)
                     /gene="folB"
                     /locus_tag="ACFM8U_001178"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC6334419.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            6332..6934
                     /gene="plsY"
                     /locus_tag="ACFM8U_001179"
     CDS             6332..6934
                     /gene="plsY"
                     /locus_tag="ACFM8U_001179"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC6334420.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLIAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHLSWIWATLLIALLVLFRHKSNISNLLKGKEGKIGEKR"
     gene            complement(6953..7078)
                     /locus_tag="ACFM8U_001180"
     CDS             complement(6953..7078)
                     /locus_tag="ACFM8U_001180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334421.1"
                     /translation="MFEHIGVVAGVECVSVTEHGMKAVCAEMPSERGNMVSDGMT"
     gene            7022..7867
                     /locus_tag="ACFM8U_001181"
     CDS             7022..7867
                     /locus_tag="ACFM8U_001181"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC6334422.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNKKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKELISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            8113..9087
                     /locus_tag="ACFM8U_001182"
     CDS             8113..9087
                     /locus_tag="ACFM8U_001182"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC6334423.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTLGHGVYVFTLNTENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(9230..9448)
                     /locus_tag="ACFM8U_001183"
     CDS             complement(9230..9448)
                     /locus_tag="ACFM8U_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC6334424.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         2278 a         2806 c         2173 g         2376 t
ORIGIN      
        1 ccgtctgaaa cagtcttcag acggcatttc catttgcaaa aaatcaagca ttgtccaagg
       61 ggacgagaat cgtacggttg ccgttgtgtt ccggtgcgga cacaatgcct tccgcctcca
      121 tctggtcaat cagacgcgcg gcgcggttgt agccgatgcg caaggcgcgc tgtacgcccg
      181 aaatactggc tttgcgcgtt ttcaggacaa cggatacggc ttcatcatac atcggatcgg
      241 tttcgccgtc gccgctgcgc ccgatgccgg gcagctcttc gctgccgccg ccgctcaaaa
      301 tatcgtcaac atagtccggc tcgccaaact gtttcaaata ttcgaccacg cggtgcacct
      361 cttcatccga ggcaaacgcg ccgtgaacgc gctgcggata ggcagtaccc ggcggcagga
      421 acagcatatc gccctgaccg agcaggtttt ccgcgcccat ttggtcgaga atcgtgcggc
      481 tgtcgatttt gctggacact tggaacgcga tacgcgtcgg gatgttcgcc ttaatcagac
      541 ccgtgatgac atcgacgctg gggcgttgtg tggcaagaat caaatggatg cctgccgcgc
      601 gggctttttg ggcgaggcgg gcaatcagtt cctcgatttt cttgcctgcc gtcatcatca
      661 aatcggcaaa ctcatcgacc acgaccacga taaacggcag tttttccaaa ggttcgggat
      721 cgtcgggcgt gaggctgaac ggattgccga ttttttctcc ccttgctgcg gcttcggcga
      781 ttttttgatt gaagcccgca agattacgca cgcccataaa gctcatcagg cggtagcgtt
      841 tttccatttc gttaacacac cagttcagcg cgtttgccgc cagcttcata tcggtaacga
      901 caggggcgag caggtgcggg atgccttcgt aaatgctcaa ttccagcatt ttcggatcga
      961 tcataatcat acgcacgtct tccggcgcgg ctttgaaaag catagacaga atcatcgcgt
     1021 tgacacccac cgatttgccc gaaccggtcg tgccggcaac caataaatgc ggtgctttgc
     1081 ccaagtcggt tacaacgggc tgtccggtga tgtcctgacc gagcgcgagc gtcagcttgg
     1141 atttggattc ggcaaactcg ggcgaattga agatttcgct caggcgtatc atttgacgtt
     1201 tcgggttcgg aagttccaaa cccatacagg ttttgccggg gatggtttcg acaacgcgga
     1261 tggaagccac gccgagcgaa cgcgccaagt ctttttccag atttagaacg gaattgccgc
     1321 gcacgccgac atcgggttcg atttcataac gcgtaatcac ggggccggaa taagaatcga
     1381 caaccttgac cttgactttg aactccgcca atttttcttc gatggtgatg ctgttttcca
     1441 acagttcttc ttcggtttgc gtcgcctcgg gattgaacag cggcggcaga agcaggtcgg
     1501 ttgtcggcag gtgttcggat accgcaccgg tttcttccga ttggaacgcc ccttcatccg
     1561 cttccgtatc cgatgcccgg cgggacgggc gttcagacgg cacatcttca aacggacaaa
     1621 ccgcctgact gtcatgcccg aacgcttcgg tttccgacag atattgcccg cttgaccgct
     1681 ctgccgcacc ctcactgccg tcatccgcaa tagcggcggt ttcctcctgc caacctccat
     1741 tcaaaacatc atcggcaaga tggtcggttt cggcaatgcg gctgcgttgc gcctgctcga
     1801 atcctgacgg cggttcatag gtacggttgt agatttccga tacgggaggt ggcggcggaa
     1861 tatcgattgc gggcatggga actttcggca cttcgggtgg ttcgaccacc caagcatcgg
     1921 gcgcggcagg ttcttcaata tggatatcgg cagcttcatc ataaaccgga ccgccgtatc
     1981 ccacagacga aacggtttcc gtgaaaacat tttcggtttc agacggcatt tcgacatccc
     2041 gtttcccgac aaccgtccga ctttccggaa tcaggcgcga aatccgcgcc tccgcagaaa
     2101 ccgttgcctt gttcggttct ttcaaattga cggaaacgcg gcggcaggca tcgcggacgg
     2161 ctgtgccgtc tgaaacggac tgcccttggg aaacatccgg ggattttgcc tccgcctgcc
     2221 ctgcatgacg gcggaacggc gcattccgtg cattttcggc tttgactgca gaaaaggcag
     2281 acggatgctg cttctcaaaa tacggctcaa atccgtaatc cgcagactcg gaaaacgctt
     2341 ctttgtccgc atcgaacata cgggaatacg tgcgttgaag cacagggtcg tccaaaccga
     2401 taatctgcag cccttccatc ggggtatcgg acacgcggac tttggggacc gctgtttcgt
     2461 ttttctcaaa tgcatcgata tagcgttttt tcgtttccct taaagccgcg ctgtttaaag
     2521 cacgcgttgc ttcttccaaa gtgatttcct taaaaaccgg acggacgggc gaaatttcgc
     2581 tttcagacgg catcaaccct tcagcaatac tccggtcgaa tgggatgcgg cggttgtcga
     2641 ttacggcagt tgcaatgtct tccgtatcgg cagcctcttc ctccgcagct tctgcttctt
     2701 ccgtttccgc ttcttccgtc ccgtttccac tgtctgaata tccgtcggat gcggtcggaa
     2761 catccgcacg gtttgcagaa gcaggcggaa cgggcctgtc cggttcggat tcgtgcgcca
     2821 gatagtttcg gcagaaacgg acgacaccga caaacagcca atacactgcc gttttgacgg
     2881 catggaaaag catcagggca agttcgggaa aatcgggcat accgtctttg atttcaggca
     2941 gctccgcctg tttttcccct ttcttttcct gccacgcaga aacctcgcgc atccactcgc
     3001 gttcggattg tgcgcggaca aaaaacaagc cggcaagcgc aagcaacaaa ataacgatca
     3061 aaactatcca aaacatatgc cgtttcccgc aagacaaacg attaaacggg tttattttaa
     3121 cgtgtttcca tcccaaaaaa aacaaccggt cggcaattct gccgaaaccg attcggcttt
     3181 cggtttgccc ccgccgacag tataatagcc gtcctttccc ttaatctttg cggcattgcc
     3241 atgattttcc aaacagtctg gttttcagat atggtactgt cggtttcgtg gattgtcctt
     3301 atcctgatac tggcagcttc cgcgccgtcc gcattccgct cgcttgcccg ctaccgttct
     3361 gcccttcccc tgtgtaccgt aattttttcc gcagcatggt gtctgaacgc ctctgccggc
     3421 ggcggacagc ttgcccagat gaactaccac ctgctcgccg tcaatctggt tgcactgatg
     3481 gtgggcactt ccgccgcctt ttggcttgcc gcgctgctga tgctgtccta ctgcctgctg
     3541 tttgccggtt ctgccggcgc atatccgccg aacgctttag tgctgattct gcccgcgctt
     3601 gccgtcaacc gcctgtcgcg tatgctggtc aaccgcctgc cgcccaatat tttcatcttt
     3661 attttcgtca acggctttct cgcttccgcc gccggcattc tgttgaccgg gctggtgctg
     3721 accggtattt tggatgctgc aaacgctttt ccgtccgaaa tattgtggac gaccgccctg
     3781 cccgtcttta ttctgctggc gtgggcggaa gccttcctca gcggcatttc aactgccata
     3841 tttgttgcac tgaagcccca ttggattaac actttcgacg acaaccgcta tcttaagtcc
     3901 gaccgcggca tatggcggta gccttcagac ggcatagaca ggcaaaacca tgctttccaa
     3961 catcatcccc ctttccatcg gtgcggcact cggtgcaacg gcgcgttggc tgctcaacct
     4021 cgccgttccc gcatcgttgt ctcccgccac gggcaacctg tttgccaact ggacgggcgc
     4081 gctgctcatc ggcatctttg ccgaaaccgt caaccatccg caatggaagc tgctgctgat
     4141 taccggtttt ctcggcagcc tgaccacgct ttccggattt tcactggaaa ccgtaaccct
     4201 gctccaatcg aatcgtcctg cttcagcact tgccaatatt ttcctgcaca cggcaggttc
     4261 gctgctgctg acttggctcg ggctgaagat agggacggca gtcaaataat caaaaaaatt
     4321 gcttcctttg gttttgacac aaactataca agtcctaaca tcttaatcat ggcgcagcat
     4381 ataagcgtgg atttgcagcc caatctatac tttctgattc tattcattaa gccattatgc
     4441 ttattcctca atcacggttt ccaccactga atgaatactt ttaggcaatt gcaaaaaagc
     4501 attccaagca attaaagtag ttttaaaagt atgcagcgaa cagcttaaat caggccagcc
     4561 gtcttcttca tcaaattcgt tgttatagaa aactactttt tcagcagaaa tatcccagca
     4621 aaaaccttgt ccacctcctg aataaacttc attgggatta ttttctgctt gatggataaa
     4681 ttctaaattt tcatctatgt ctttttgtag ataaaggtct cctgttaaaa cataaattaa
     4741 gtattcatat attctatctt taattatttc attattttct tcatctaaaa atatacatct
     4801 ttttaatagt tccccattaa attcataaac atcgtaataa gctttaaaaa tagatatcat
     4861 atcatttcct tatcttccat aaatcggctt cggttgttta aataccgccc gtgccaatta
     4921 ccgttctcaa attcaaattg ggcttttagg tctataacaa agaaaaagcc ttggcaggat
     4981 aaacctacca aggcttttta ttaaaaaaaa cgctctatta ctgtgtctaa aaatgcaaaa
     5041 aaagtaacca tcataaccct tttgccaaaa tcaatatcat catccgatga atgaattgac
     5101 ggtatataag aaagaattaa taagctcata aaccggtaag aaaaatcagt aacaaagata
     5161 ttcattatta actattctta aaaatgatca cgttcgtttg ggtaattatc agcccatacc
     5221 ataccctact cctattacta aagcacgaat gactttaaag gacgcgccca gagacaagcc
     5281 gaaaaggcat atctcatccg ccaaacaaaa tgcccgccga cttactccgg caggcagttc
     5341 aacaccctgt caatccttca tcagccagta ttgcaaaccg attaatgtct tgccgtcttt
     5401 aatttcatcg tttgccaatg cctgacggac ttcttctttc gacatcaata cggtttccgt
     5461 aatctcgtct tcgtcattgg caagcgtact gcccaagcgc acgccttccg cctcgaacag
     5521 atacattttt tcattgcaaa aaccgaccgc cgtataaaag ctgtacagca ggcgcacgct
     5581 gtcggcaaca taaggtgttt cctccgccaa ttctcgcagc gcacacgctg ccatatcctc
     5641 gcctgccaca tccagcttgc ccgcaggaag ttccaaggtc gcctgatttg ccgcataacg
     5701 ccactgccgc accaaaacga ccttttcatc atccgttacc gccaacacgc acgccgcgcc
     5761 cggatgacgg atgacgatac gctgcccttc attgccgttg ggcaacctga ccttatccct
     5821 gctgatactg acgaaaccgc cctcgtaaat ggtttcgccg cctaatttta cttccctcaa
     5881 atccacttca tatctccttt ccgtttccta ttctgccgtt caatcttcac gcttgccgcg
     5941 ctcgatttcc acgccaacct cgcgcacgcc ttccaaaata cccggcttga cgattttcac
     6001 gcgcacccac accgcgccga aatatcccaa aaccaaatcg gcaatatatt ccgccaacgc
     6061 ttccaaaagc aggaaatcct gttctttcag atgtcggcgc aatgtttcgc atacctcggc
     6121 ataatgcacc gtattggcaa tatcgtcgtc cgaacccgct ttctcgggaa cgccgatgtc
     6181 caaatcgaca atcagggtct gcaaccgttc gcgttcccag ccgtacacgc cgataagcgt
     6241 atccgccttg atgccgtgca aaaagatttt atccataatc cgccccgttt tttgctaaaa
     6301 taaccgcacc attctaagta aagtaccaca aatgttcaac ataccggctg ttgccgtttc
     6361 ctatctgatc ggctcacttt ctttcgccgt catcgtttcc aagtattacg ggatggacga
     6421 cccgcgcacc tacggatcgg gcaatcccgg cgcgaccaat gttttacgca gcggcaaaaa
     6481 aaaggcagcc gcgctgacgc tcttgggcga tgccgccaaa ggtttgattg ccgttttgct
     6541 tgcacgcgtg cttcaagaac cgctcggttt atccgacagc gcaatcgctg ccgtcgcact
     6601 cgccgcgctg gtcgggcata tgtggccggt gtttttcgga tttaagggcg gcaaaggcgt
     6661 ggcaacggca ttgggcgtgc ttttggcact ctctcccgca actgccttgg tctgcgcgtt
     6721 gatttggctt gttatggcat tcggcttcaa ggtgtcctcc cttgccgcat taaccgccac
     6781 aatcgccgcc ccccttgctg cgctgttttt tatgccccac ctttcttgga tttgggcgac
     6841 gctccttatc gccctgctgg tgttgttccg ccacaaaagc aatatcagca acctgcttaa
     6901 aggcaaagaa ggcaaaatcg gcgaaaaacg ctgattcggc atttgttttc cattacgtca
     6961 tgccgtctga aaccatattt ccgcgttcag acggcatttc cgcacaaacg gctttcatac
     7021 catgctcagt tacagacacg cattccacgc cggcaaccac gccgatatgc tcaaacattt
     7081 caccttgttt ttagtgctgc aatatttcaa caaaaaagac aagccctact ggtacatcga
     7141 cacgcacggc ggcgcgggtt tgtacaatct cgaaggcagc gaggcacaaa aagtcggcga
     7201 ataccggcaa ggcatcgccc tgctccgaca ggcgcaaaac ctgcctgccg aactctctga
     7261 ctttgccgcg cacatacaaa aaatcctgcc ctcgcccgaa ctttactgcg gttctccgtg
     7321 gctggcgcaa tcgctgaccc gcgtcggcga caaattacgc ctgtttgagc tgcaccccac
     7381 cgactttgtc catctgcaaa acaatatggg cgaagcagga ttgggcaaac gcggacaagt
     7441 gttgcgcgaa gacggttaca aagagctgat ttccctgctg ccgcccccgc cgcgccgcgc
     7501 caccgtcctc atcgacccgc cctatgagga aaaacaggac taccggcgtg taacggaaac
     7561 gctgaaagcc gctttaaaac ggtttgagtc cggctgttat ctcatttggt atccctgcct
     7621 cagccgcgaa gaaagccgca agctgcccga agaattgaaa aaactcctgc ccgacaacta
     7681 ccttcacgcc gaacttcacg tccacgcgcc gaaagccgac ggtttcggta tgcacggcag
     7741 cggaatgttc gtcatcaatc cgccctatct gttggcggaa caattggcgg caaacctccc
     7801 cgccctgacg cggctgctgg cgcaagacga aggcgcgcgc taccttttgg acagcaaaat
     7861 ccgctgaccc cgatgccgtc tgaaaccctt cagacaacat ctcccgtcaa ataaacaaaa
     7921 ccggacgata taccggcagt cggcactttt aagaataaat atcccctcat ccgcctgccg
     7981 cgttccgact tgccccatgc cgttttttta cactacaatc ccctagacta tcctgcaaaa
     8041 cgcaccaaag gaatacgcca caataacctg ccgcctttca gacggcacaa acctttccga
     8101 aacgagcaaa ctatggacac actgacccga ttcctccccg aacacctgca acaaaaccag
     8161 ctgcccgaag cactcggcgg cgttttactg tccgttgttt ccgcctgcac cgaaatcaac
     8221 gccaaagtcc gcctcggcgc actggccggc gtattgggta tggcgggcac gggcaatatt
     8281 cagggcgaag accaaaaaaa actggatgtt atcgccaaca acatcatgat tgacacactc
     8341 aaagccaacc ctgccgttgc cggtttggca agcgaggaag aagactcttt cgtaagtgcc
     8401 ggtgaaaacg gacgctatct cgtcctattc gaccctttag acggatcgtc caatattgat
     8461 gtcaacattt ccgtcggtac gattttctcc atccttgaaa aacccgaagg cgcattggca
     8521 accgaatcat tccttcaaac gggcagacag cagcttgccg caggctatgt cctctacggg
     8581 cctcaaaccc agctcgtatt cacactcgga cacggcgtgt acgtgtttac actcaatacc
     8641 gaaaacgaat ttgtcctgac caaagaaaac ccgaaagtac ccgaaagtac caaagaattc
     8701 gccatcaata tgtccaaccg ccgccactgg ctgccccccg tccaacaata catagacgag
     8761 ctcttggcgg gcgaaactgg cacgcgcggc aaaaactaca atatgcgctg ggtagccagc
     8821 atggttgccg aaatccaccg catcctgatg cgcggcggcg tgttcatgta tccgcaagac
     8881 aaacgcgacc ccgccaagcc cggcaaactg cgcctgatgt acgaagccaa ccctatgagc
     8941 ctgatactcg aacaggcggg cgcatcggca agcaacgcat atcaagccat gctcgacata
     9001 cagcccgaaa acctgcacca acgcgtcgcc gtcttcatgg gcagcagcga agaagtcgcc
     9061 tatctcgacc gcctgcacgc aaaatagaca gacagccgtt cacacaagta cacggcggcg
     9121 gcaatccgat accgccgccg ttatttcaaa cagcacaagc cttccggtca aatgacaaaa
     9181 atgccgtccg aacacatttc agacggcatt tcaaaaactc aaacccgaat tattttcccg
     9241 ctgctttttg caggatggcg tacaactcgt ctttcagttt caatttggct tttttcagct
     9301 catcgatggt ttccgcaccg ctggtaaccg gattgttgac cagaccggta attttatcgt
     9361 ccagctcgtt gtgttcgtcg aacagacggg cgaagcggga attttcctgt ttcaatttgg
     9421 aaatcaaatc acggtattct gggaacatat cgctttcctc tcttggttta cggttaataa
     9481 agtcaaactg aatctttaca ttaattataa caaagataat atttttatcc agtattttat
     9541 cggatacggc aaaattttct tttaacaccg ccgtccggtt gataaaaacg ttgacaatat
     9601 cacgctttac gcataaattt tcagcctttc cta
//
LOCUS       DAXHSJ010000069         9373 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000069 DAXHSJ010000000
VERSION     DAXHSJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.069"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(222..1658)
                     /locus_tag="ACFM8U_001184"
     CDS             complement(222..1658)
                     /locus_tag="ACFM8U_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6334425.1"
                     /translation="MFIKSICLSNFKGFIGDNHQIKFKIPDGATAGSGLNIFIGENNS
                     GKSSIFEAINFLRNGIKEDEAYRIKSKLADDTQPNDACVELAFCGNIQNAITYFVQRN
                     KQAAFNNAINNEQNLKAKRNTVSCKKLDLWDNESQDYSNPSGIDAPFKALFETNFIWA
                     DTNPNDEAAFGSTTLCGLLLKEIAQAHINSAEYQTFRDSFNQVFNSPASNLRQQIATI
                     EQKVQRIFTEQFGQADIHFRFDELKIDSFFKNASIFIDDGVDIPMSEKGNGMQRSVAL
                     ALLQVYAEELAHDEEQGQTKPFYLFIDEPELCLHPKGQTKLLEALLEISRTKQVFLTT
                     HSPYFLVTPHLSNVGLFIFRKDGISNIVEDASLEPMFPWSPTWGEINFKAYKLPTVEL
                     HNELYGKLQHDCGQFREKDFEQWLQSRNIQFTKQWIRENNGAAQAPYDVTLQTFIRNH
                     IHHPENRRNALYTENELRQSIEEMIRLL"
     gene            complement(1807..3186)
                     /gene="norM"
                     /locus_tag="ACFM8U_001185"
     CDS             complement(1807..3186)
                     /gene="norM"
                     /locus_tag="ACFM8U_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC6334426.1"
                     /translation="MLLDLNRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            3521..4672
                     /locus_tag="ACFM8U_001186"
     CDS             3521..4672
                     /locus_tag="ACFM8U_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC6334427.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            4776..6074
                     /locus_tag="ACFM8U_001187"
     CDS             4776..6074
                     /locus_tag="ACFM8U_001187"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC6334428.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMEGWKESTFGIKNYEE
                     LPDNAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            6539..6922
                     /locus_tag="ACFM8U_001188"
     CDS             6539..6922
                     /locus_tag="ACFM8U_001188"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6334429.1"
                     /translation="MPKSLSLQDVQLRFSSKFPDKTVLKFTKNYEPVTIQCPLHGSVV
                     YGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGEIYEFASTLAASKFIGCSN
                     STLAVRLSGRTPFDRLIRYRYRLVK"
     gene            6936..7346
                     /locus_tag="ACFM8U_001189"
     CDS             6936..7346
                     /locus_tag="ACFM8U_001189"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6334430.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            7463..7855
                     /locus_tag="ACFM8U_001190"
     CDS             7463..7855
                     /locus_tag="ACFM8U_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6334431.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            8075..8455
                     /locus_tag="ACFM8U_001191"
     CDS             8075..8455
                     /locus_tag="ACFM8U_001191"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6334432.1"
                     /translation="MTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQLLYY
                     DTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALNTPYG
                     SIRTKLDGRSNPDNLVCNRYKVLL"
     gene            8569..9129
                     /locus_tag="ACFM8U_001192"
     CDS             8569..9129
                     /locus_tag="ACFM8U_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334433.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
BASE COUNT         2535 a         2259 c         2245 g         2334 t
ORIGIN      
        1 ccatatgttt ttatcaaaac aaagattaaa caggtttatt gctgtttatc ctcaaattgt
       61 ccttatttca gcaggcgatc aaagtttttt agtttttacc atcatcaacg gttacccaaa
      121 aattttgaat aactgaattt tcttctttgg gctagaaagt gtggcttcag acgacgtttt
      181 gaaaacattg agagaattga ccgcactttt tactccttca actataacaa tctaatcatt
      241 tcctcaatag attgtctaag ttcattttcc gtataaagtg catttcgccg attttcagga
      301 tgatgaatat ggttgcggat aaatgtttgc aaggttacgt catatggagc ttgtgcggct
      361 ccattgtttt ctcgtatcca ttgtttagtg aactgaatat tcctactctg taaccattgt
      421 tcaaagtctt tttctcgaaa ttgcccacaa tcatgttgta atttcccata taattcatta
      481 tgtaactcca cggtaggtaa tttatacgcc ttgaaattaa tctctcccca agttgggctc
      541 caaggaaaca ttggctccaa tgaagcgtct tcaacaatat tgctaattcc gtcctttcta
      601 aaaataaaca gaccaacatt acttaaatga ggtgtaacca aaaaatatgg ggaatgcgta
      661 gtcaaaaata cttgtttcgt tcttgaaatt tctaacaacg cctcaagtaa tttagtttgc
      721 ccttttggat gtaaacaaag ctcaggctca tcaataaaca aataaaacgg cttagtctga
      781 ccttgctctt catcatgcgc taactcttct gcatagactt gaagcaaggc taatgcaaca
      841 gaacgctgca taccattacc tttttcggac attggaatat ccacaccatc gtcaataaag
      901 atagaggcat ttttaaaaaa cgaatctatc tttagttcat caaatctaaa atgaatatca
      961 gcttgtccga attgttcagt aaaaatacgt tgaactttct gttcgatagt agcaatttgt
     1021 tgacgcaaat tagaagctgg tgaattaaat acctgattaa aactatcacg aaatgtttga
     1081 tattcagccg aattgatatg cgcctgagca atttctttca gtaacaatcc gcaaagagtt
     1141 gtagacccga aagccgcttc atcattagga tttgtatctg cccaaataaa attagtttca
     1201 aaaagagcct taaaaggagc atcgatccca cttggatttg aatagtcctg agattcgtta
     1261 tcccacaaat ccaatttctt acaagaaact gtatttctct tagcttttaa attctgctca
     1321 ttattaattg cattattaaa agctgcttgt ttattccttt gaacaaaata agttattgca
     1381 ttttgaatat tcccacaaaa agccagctca acgcatgcgt catttggctg agtatcatct
     1441 gccagtttac tttttattct ataagcttca tcttctttga taccgttcct taagaaattt
     1501 atagcttcaa aaatagacga tttcccagag ttattttctc cgataaaaat gttcaaacca
     1561 ctaccagcag ttgctccatc aggaatttta aatttaatct gatggttatc accgataaac
     1621 cctttaaaat tagataaaca aatacttttt ataaacataa cggtttcctt atcttaagtt
     1681 agaaatagag attaagctaa aacaccaaac tctctccctt caaaaactct aaattctcct
     1741 agctcaaccc tgccacatca tctattaata ttgtaattag cagacttcat cctacccatg
     1801 gagttttcag acagccttat gcgatctgac catctcccta ctgcacaatt ccaagcacca
     1861 caccaaggcg atggcggcga tggtgagcga ggcaatcaat gccgtccaga agccgtaaat
     1921 gcccatatcg aaacggtagg cgagcaggta gcccggcagc agaccgcagc cccaaaaggc
     1981 ggcggcgtgg atgaacatcg gcacctttgt aactttgtag ccgcgcaagg cgtaggaggc
     2041 gatacattgg gtgaagtctg ccggttggaa caagccggcg aacagtaaga cggtggcggc
     2101 gatgcttaaa accgccggat cattgttgta catacttacc agcggcgaac ggaataatac
     2161 caaggaaagc acggtaatca cggcgagcat ccatcctaac accagtgaca cgcccgaaat
     2221 ataacgcgcc cgcgaaaatt cgcgccgccc aagcgaaaag ccgatgcgca ccgtccccgc
     2281 cgagccgacg ctttgcggaa tcatatagag aatccccgac aaactgatgc cgacctgctg
     2341 cgccgccaca taatcctcgc cgaaaggcgc aatcaaaaac acgataaacg aaaacgcgct
     2401 ggcttccaaa aaataagaca gcccgatggg tgcgccgatt ttccaaatct gtttgaacac
     2461 cgcccaatcc ggtttgccga atttcgccgt cagtccgaat gggcggaaga aattttcctt
     2521 ggcgatataa atccacaatg ccagcgcgct gaaccaaaac accgccatcg tcgccagtcc
     2581 gcagcctgcg ccgcccaaag cgggcatacc gaatttgccg taaacgaaaa tatagttcag
     2641 cggcacgttc aacacaaacg ccgcaaagct gaccaacata atcaggcgcg ggcggttcag
     2701 gctggaggcg taggcgtgca gtgcgcggtg taccattgcc gccggcatcg ccaagctggt
     2761 gaacagcata tactgcgcca ttgtgccttc cacataatcg ctcaaggtca gccagttgcg
     2821 gaacggcgta atcgccgccc acatcaagac catgccgaac acgcccaaaa acagcccgaa
     2881 ccaaatcccc tgccgtcccg tttcgcccac ttcgtcggtt ttacccgcgc cgtaaagctg
     2941 ggcaatcatc gggttcagcg ccgccataat gcccataaag gtaatataaa ccgtggcaaa
     3001 cgcgctgctg cccaaagcca ccgccgccaa gtcttccttg cccgcaccgc ccgccatcac
     3061 ggtatcgaca aaaccgatgc ccacctgcgc gacctgcgcc aacagcatgg gcagggcaag
     3121 ggcggtcagc aggcggattt ctttcaggaa gacggaaaag gaaaagcggt tgaggtcgag
     3181 cagcataagt gttcaatcaa caaaaatgcc gtctgaagca gaaaacggca gcaggaagtg
     3241 atgagaaata atggcgcaca ttatatagta aaaaaatgcc gtgccgtcag acggcggata
     3301 cagggtataa aagtatattc agattgtgtg tattttatgg taaagtttgg ttttaacgac
     3361 ttgacggcat tgagccgtcg gacaggggct gttcggattc tgaatcggaa ggaagcatcg
     3421 ccgttttgac agcggcgtga tgcgttgcgg caaagatgcc gtctgattgc ggatcgggca
     3481 gccttttgta tttacatgat aaaatagaag gcagattctc atgcagacat ggcagcttcc
     3541 ggaacatatc gccgacgtac tgcccacgaa cgcgcggcag cttgaaagcg cgagggagca
     3601 gttgttggca ctgttccgcg tacacggtta tgaactggta cagcctccgc tgatggagta
     3661 cgcacattcc ctgctgacgc atatcgatgc ggggctttcc ctgaaaacca ttttggtaac
     3721 ggacaggctc agcggcaggc agttgggcat acgcgccgac atcacgccgc aggtggcgcg
     3781 tattgatgcc catcttttat ccgccaacca agggattaac cggttgtgtt atgccggtcc
     3841 ggtgttgcac gcgcagcccg acggtctgct gaatatgcgc gaacccttgc aggcaggggc
     3901 ggaaatgtac ggttttgccg acatccgtgg cgacatcgag ctgatagacc tgatgctgaa
     3961 aagcatgaaa attgccgata tgggcaaagt gctgctttcg ctggggcata tcggcatatt
     4021 ccgcgccttg tccgatgcgg cgcatttgga tgcggggcag tccgcaacgc tgcttgcctt
     4081 gatgcaggat aaagataccg gggcggtcga agcgcaggtc aaggcttgga agctggacgg
     4141 catgtgggca aaagcattct cgctgttgcc gcgcctgtac ggcgggcgtg aagtgttgtc
     4201 cgacgcgcgc ggacggttgc cggatttgtc ggcggtcggc ggcgcgttgg gcgaattgca
     4261 ggcggtgtgc gacgcattcc ccgattgtga aatccatatc gacttgtccg agctgcgtgt
     4321 cgacaattac cacacgggct tgctgtatgc cgcctatgcc gccgatttcc acgacgcggt
     4381 cgcgcgcggc gggcgttatg acggattggg cggatatttc ggcagggcgc gcccggcaac
     4441 gggattcagt ttcgacttgc gcagctttat cgggcgtttg cccgccatcg aacggcagcc
     4501 cgccgtgttg gtcgatgcgg aagatgccga agcggcgcgc gaagcggtcg aagccttgcg
     4561 tgaacaaggg cagtgtgtcg taatcgatta cggtatcgga cacaatgttt cggaagagct
     4621 tgcaggccgt ctgaaaaaga cggacggcgt ttggcaggtc gtgaaacgct aaatacccgt
     4681 tcatggcgga tgaaaggcaa atcgtggcgg ggcgcaaagc cgcaccggtt tggggatttc
     4741 cgcaataatt tttaatatcg ataggttata tggctatggc taaaaatgtt gtagtaatcg
     4801 gcgcacagtg gggcgacgag ggtaaaggta aaatcgttga ctggctggcg gaagaagccg
     4861 gcggcgtggt gcgcttccaa ggcggccaca atgcgggcca taccttggtt gtcggcggca
     4921 aaaaaaccat tttgcgcctg attccgagcg gcatcctgca tgaaagtttg gactgcttca
     4981 tcggttcggg cgttgtcgtc tcccccgaag ccctgttggg cgaaatcgac gagttgaacg
     5041 cggcaggcgt gaaaaacgtc gaaggccgtc tgaaaatcgc gccgacctgc ccgctgatcc
     5101 tgccttatca catcgcgctc gaccaagccc gcgaagcatc gcgcggcaaa ggcaaaatcg
     5161 gcacgaccgg ccgcggcatc ggccctgcct acgaagacaa agtggcacgc cgcgccattc
     5221 gcgccgccga tttgctgcat cctgaaaaac tgcgtgaaaa actggatgcc gtccttgcct
     5281 attacaatgt ccaactgcaa catctgcaca atgccgagcc ggttaaagcg gaagacgtga
     5341 tggcggttat cgaaaaagtc gcgccgcgca ttgcgccgat gattaccgac gtgtcccgcg
     5401 tgttgaacga gaaaaacaaa aacggcgaaa aactgctgtt tgaaggcgcg caaggtgcgt
     5461 tgttggacat cgactacggc acttatccct tcgttacctc gtccaactgt ctggcgggag
     5521 ccgcttccgc aggcgcgggc gtaggtcctc aaatgctgga ttatgttttg ggcatcgtca
     5581 aagcctatac cacgcgcgtc ggttcaggcc cgttcccgac cgaattgttc gacgaagtag
     5641 gcgcaggttt ggcggaacgc ggacacgaat tcggctcggt aaccggccgc gcacgccgct
     5701 gcggctggtt tgatgccgcc gccctgaaac gctccatcca aatcaacggc atttccggta
     5761 tgtgcattac caaactcgat gtaatggacg gcgttgaaac catcaatatc tgcgtcggct
     5821 acgaattgcc cgacggcggc aaaaccgata tcctgccttg cggttcggat gcggtggaaa
     5881 cctgcaaacc gatttacgaa acaatggagg gttggaaaga atcgactttc ggcatcaaaa
     5941 attatgagga actgcccgat aatgccaaag catatttgaa acggattgaa gaagtctgcg
     6001 gcgcgccggt cgccatcgtc tccaccggcc ccgaccgcga agaaacgatt gtgctgcatc
     6061 atccgttcgc ataaggtttt gcagtaaaat tgccgtctga agccctaatc cgccgttggt
     6121 cataaatagt agggtatcaa catttcgggc tacaatggta cgtcagccaa tgccaagacg
     6181 tgccagcctg attggttgat gtgtgtacta ttgcataggc ggttaagcca tgcaggagat
     6241 gaaagtgtat atttcgcgca aagcccatta gccgcaagcg aggggcgggg tgcgcgagag
     6301 agaggggggc aggtgctaag tcattggtta tctccttact atctatctaa ctacgggtgc
     6361 attatgcgcc tatgcctgtt gtttgtcaaa cactatacag ttaaaatgtg taaagattta
     6421 gtaaggacat ataccctttt ctttacattt agctccatcg gataccagtg cgttcagaga
     6481 aaatttgaaa atttctatat tttggttgta taatgctttc attttagaaa ggtctataat
     6541 gccaaaatca ctctcgctac aagatgttca gttaaggttt tccagtaaat ttcctgataa
     6601 gacggttttg aagttcacta aaaactacga accggtaaca atccaatgcc ctttgcatgg
     6661 gtcggttgtt tacgggaatc tacaagctgc catgaaatcc tcaacaggat gtcctgaatg
     6721 tactcgaaat cccgaaaagc cctctccaaa cgccgttgcc attaggttgg aggacacaga
     6781 gacaggggaa atctacgagt ttgcaagtac actggctgcc agtaaattta tcggttgctc
     6841 caacagcact ttagctgtac gtttaagcgg acgcactccg tttgaccgat tgattaggta
     6901 tcgttatagg ttggtaaagt aagttcacaa ccactatggc acttacattt actcaagcag
     6961 tttctaagct aacctctaaa tttccacatt tgaatcttgt ggagttcaat ggcgttcgtt
     7021 acccgacggt aatcgtctgt cctatacacg ggagggttac ttgctctaca ttcaaaagta
     7081 tgttggactc taaaagtggg tgtccaaaat gtgcatctta tggtgtcaat tcccacaaaa
     7141 ttccagaaga tacaatagat aaattatcta aaaatacagt attggaggat actgtaactg
     7201 gcgaaacact tacattcccc tcaagagcat ctgctgcaag gtcattgggt ataaacccag
     7261 cagctatcac tgaccgtata aaaggtcggg ttcacacaga gactttactt gcagggaggt
     7321 ataaggttca catctgcact aaatgacgta tacacttttt aacagtgtat acccctcgcc
     7381 actatcaggt gcttctcgaa aaatttgaca tttttataaa tttgctatta aatccatttg
     7441 acaatcaatt ttggagtctc aaatggccaa atctttcaac caagcagctt ctgaacttac
     7501 tgatatattc cctaatatct ctctaaccgg ctttgacggt gtgaattacc cagtaaccgt
     7561 taattgtcct atgcacggga acgttcgtta ttcgacattt aatgccctta taaagtcaaa
     7621 gtacgggtgt cctgagtgtg ctaagatgtc aaaaacccaa acacctccaa atgtagggaa
     7681 gcccctcctc atcctcgaca caacgaccaa cgaaacactc acgttcccaa gcgttactgc
     7741 cgcaggtgct gctttaggtg tacatttcca acaaatcaat cacaggctca agggtcgaac
     7801 gtcgcccgac aaccttattt caaacaggta caaagtgctt gggtacgata ggtaaggttc
     7861 acaacaccct aaatcctacg aaccaagctc gacggacgtt caaatcccgg ttagagttaa
     7921 tttatgtaaa gatttagtaa agacgtatac catttttctt tacattgtgc ttgcgcagga
     7981 tttcaggtag gtctcaaaaa atttgaaaat ttctatattt tggttgtgta atccatttgc
     8041 acataaccta tggagacaaa ttatgggtaa gcgaatgact ttcgataccg ccaaatcacg
     8101 ctttcaagag aaatttccac atttagaatt gttggagttc agtggcattt ataaaccttc
     8161 cagtgttaga tgtcctacgc acggggttgt ccaacttttg tattacgaca cagctataaa
     8221 gtcaaagtat gggtgtccgg agtgcgggaa acttaaaatg aaggaaaata cgcctcccca
     8281 aaaccaaaaa cctgtctcca tcctcgacac cgccacaggc gaaacactca cgttccccag
     8341 cgtacaagct gccgccaaag ccctaaacac cccctacggc tctatacgaa ccaagctcga
     8401 cggacgttca aatcccgaca accttgtctg taacaggtat aaggttctgc tataatcaac
     8461 ctatggaaaa tattgaagaa tacgcactgc tgtctcccga agccctgctg gaacgcctgg
     8521 ataccgtttt gagtatcaga atcggcggca agggttggga atctagttat gaccgccaac
     8581 tttgcacaga cgctggtcga aatacaggac agtctgtaca gggttgtgtc aaccgtccaa
     8641 tacggggatg acaacctcaa gcggttgaca gcggacaaac ggaagcagta tgagttgaac
     8701 ttcaagattt ccgagggttc tacgcgtgta gagtccgact ttaaagagac tttggttcgg
     8761 ttcggtagag atatgcttca agatatgccc cctaaaatcc gttcggcaac gctggtagcg
     8821 ttgacgaccc tgcttgtcgg aggggcgttg ggttacggtt atttggaata cctgaagcag
     8881 gttgcttcgg aagggtatca gaccgagcgt ctgtataatg ccgtcgaccg tcttgcagaa
     8941 tcccaagaac ggataacgtc cgccatcctg aagggtgcta gaggtgccga tttcgtgcaa
     9001 atcggcagac gttcctacag tagggaggat atatcggagg caaatagacg tgcagagcgt
     9061 gtcccgtatg gcgcagagtt ggtttcagac ggcaatttta ccgctgtttt atctgatata
     9121 ggggattaac aaaaatcagg acaaggcgac gaagccgcag acagtataaa tagtacggaa
     9181 ccgattcact tggtgcttca gcaccttaga gaatcgtttt ctttgagcta aggcgaggca
     9241 acgccgtact ggtttttgtt aatccactat attttctgtc cggatacggt ttatcagggt
     9301 atatcaatgc ggcgtatccg gtgcggaaat ggatacggtt ggtgtcggta tggaaacctg
     9361 atgttttcag aca
//
LOCUS       DAXHSJ010000070         9285 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000070 DAXHSJ010000000
VERSION     DAXHSJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.070"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(63..389)
                     /gene="cas2"
                     /locus_tag="ACFM8U_001193"
     CDS             complement(63..389)
                     /gene="cas2"
                     /locus_tag="ACFM8U_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFC6334434.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     gene            complement(382..1296)
                     /gene="cas1"
                     /locus_tag="ACFM8U_001194"
     CDS             complement(382..1296)
                     /gene="cas1"
                     /locus_tag="ACFM8U_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HFC6334435.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNDESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QFWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLVVIHLYEQGRLKTELTPSIKQHLIKTLHCQTSIERQHFSTLAAIDKMVS
                     SFQAGVTDKNAKQLKLPEILPLKAYQYE"
     gene            complement(1362..4607)
                     /gene="cas9"
                     /locus_tag="ACFM8U_001195"
                     /gene_synonym="csn1"
     CDS             complement(1362..4607)
                     /gene="cas9"
                     /locus_tag="ACFM8U_001195"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HFC6334436.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEEENPIRLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELNLLFEK
                     QKEFGNPHISDGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPTEPKAAKNTYTAER
                     FVWLTKLNNLRILEQGSERPLTDTERTTLIDEPYRKSKLTYAQARKLLELDNTAFFKG
                     LRYGKDNADASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRVQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIDLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAATKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEEGFKERNLNDTRYVNRFLCQFVA
                     DHMLLTGKGKRRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKAHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDES
                     ISVLRVPLTQLKLKDLEKMVNREREPKLYDALKARLETHKDNPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGGKYYLVPIYSWQVAKG
                     ILPDRAVVAFKDEEDWTVMDDSFEFRFVLYANDLIKVQLKKDSFLGYFSGLDRATGAI
                     SLREHDLEKSKGKDGMHRIGVKTALSFQKYQIDEMGKEIRPCRLKKRPPVR"
     gene            complement(4885..8319)
                     /gene="dnaE"
                     /locus_tag="ACFM8U_001196"
     CDS             complement(4885..8319)
                     /gene="dnaE"
                     /locus_tag="ACFM8U_001196"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC6334437.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     EAYVGKDRNVHHAELNPEWLDNGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLVKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMETEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKSTTGDIVDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            8644..9084
                     /locus_tag="ACFM8U_001197"
     CDS             8644..9084
                     /locus_tag="ACFM8U_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6334438.1"
                     /translation="MRHRQCGFVGEQDLQPAAGQDFFDRLAAYGVSDGIFTEQAVRIH
                     RWFCGNFGYGGGRCYNNGLFLQGNIMEATVYLEDNEYIALCDLLKLAGLAESGGQAKA
                     FIAEGLVSRNGETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            9062..9271
                     /locus_tag="ACFM8U_001198"
     CDS             9062..9271
                     /locus_tag="ACFM8U_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC6334439.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSEDENAV"
BASE COUNT         1979 a         2256 c         2518 g         2532 t
ORIGIN      
        1 ccccaacggg aaatccttat tctataagga tttcccgttt tatttagtct gaaaaaatgg
       61 gcttaaaata acaataattg gtctgaattg accttttttt cttgggtttt cagctcgccc
      121 aacagcagtt tcatggcggc atattgcttt tctgttatct ccaaacagcg gattgagcct
      181 tcttgcggaa ggtttgcaca tagtctattg tggtgttttt gcaacgaatc gcggcctttg
      241 acgatacggc tgtatacgga aagttgcagc atttggtatc cgtcttttaa taaaaactgg
      301 cggaattgat tggcggcttt gcgctttgcc gccgtgataa ccggtaggtc gaagaagacg
      361 ataatcctca taaatttggc ctcactcata ttggtatgct ttcaatggta ggatttcagg
      421 cagtttcagt tgtttggcgt ttttatcagt tacgccggct tgaaatgacg aaaccatttt
      481 gtcgatggca gccaaggtgc tgaaatgttg ccgttcgatg ctggtttgac agtgaagggt
      541 tttgataagg tgttgtttga tgctgggcgt cagttcagtt ttcagacggc cttgttcgta
      601 caaatgtata acgaccagat cggccaatgg gcgtaagggt tcgataaagt catcggcgag
      661 attgaatgga ttcaactcac ttctatgaaa caagcctaat gcaggcaacc aaccgtataa
      721 ggtcagggcg cgtgcaactg cagcccggag cacagcgtag gtatagttca atgcggcatt
      781 gacggcgttg ttgtcattgc gggtaaattt ttcgccaaag agggcttgga agtagagggc
      841 ggcggcttgg gcttcgcggt ttcctgtatc gcccgagcgg acttcagatg ccaaagtgcg
      901 cagccgtttg gctgcaagat cattgccggt ttcgtctgcg acaaatgctt gattcaggat
      961 tttttggcgg acgatatgtt gccagaattg cttttttaag ggttcgctta tattcagctg
     1021 cagtttgagg attttaagtt ggcggtgata ttgggcataa ggcagccatt gtccgcaggg
     1081 caggaactgc tcgtcacatg tcagtaaggt tgcgccatgt tcggctaagg ctgacagaag
     1141 tggagcggta atcagggttt cgcggttttc gatgatgata acggcgatgt cttctaatgg
     1201 tacggtgtgg gactcgtcat tttgttgaat cagcagttgc cggcgttgta aggatagttt
     1261 gccgccgttt tgaatgagca ggctgcgcca agtcatgttt ttccccttat ttttatataa
     1321 aaatccctgc ttcaggccgt ctgaaacagg gatagattaa tttaacggac aggtgggcgt
     1381 tttttcagac ggcatggtct gatttctttg cccatttcgt cgatttggta tttttgaaag
     1441 gaaagggcgg ttttaacgcc gatacgatgc ataccgtctt taccttttga tttttccaaa
     1501 tcatgctccc gaagagaaat ggctccagtg gctcgatcta atcctgaaaa gtaacctaaa
     1561 aagctgtctt ttttaagttg aactttaatt aaatcattcg cataaagtac aaacctaaac
     1621 tcaaaactat catccattac cgtccaatct tcttcatctt taaaagcaac aacagcccta
     1681 tccggtaaaa tccctttcgc tacctgccaa ctgtaaatcg ggaccagata atacttgccg
     1741 cctttctcaa acacatctac gcgcaccatg gttgcgttgt cggcaatacc gttatggttg
     1801 cgtacccata cgccggtttt ctgtacttgc tctacgcgta cggcttttac ctgttgggtg
     1861 cggttgcctg ccttatcgta tttgtaaaac ggctcggcaa aggctttggc aggattgtct
     1921 ttatgcgtct ccagccgtgc tttcagtgcg tcgtatagct taggttcgcg ctcccgattg
     1981 accatttttt ccaagtcttt cagttttaac tgtgtcagcg gtacgcgcaa cacgctgatg
     2041 ctttcgtcca gtcgtttggc ggatttgacg gtctccatat ggccttgccc gctcatcttc
     2101 cgattgggcg cgcgtgaaac aaacagtggt gtaacgtatt cgtgtacggc ttcgggacga
     2161 gaagataatt tttcagcgag caatgttcgc agtttttctg gggtatcggc ttcttcaaat
     2221 tcgggtttgc cgtccggttt gccgaagacg cgaatcatga cttcttgtgc gaaaaattcc
     2281 caaggttgcg ggaagtgtgc tttttgatgc agcacttctc ctgtttcttt gtctatggtt
     2341 ttgccgtcaa acgcgttcat ctctttatag cgtacgaatc gggtaatttt ctgctgcatg
     2401 gcaacggtcg agcaggcaac gacgaccgcg tccaaggcgt gatggcggtc gttttccgca
     2461 cgcactttgc gtaatcccca aaagccgcgc aacagattgg taatttgtcc gtttgaggca
     2521 aagacacgtc ttttcccttt acctgtcagc agcatatggt cggcaacgaa ttggcacagg
     2581 aaacggttga cgtagcgcgt atcgttcaga ttgcgttctt taaagccctc ttcatcgaat
     2641 ttttgcagca gaatccgttg ttttttactg cgtgggaaac ggctggtttc gacacgcgct
     2701 ttaaattcct gccattcgcg gctgttgtct ttgccgttga agtattcgta aggggtttga
     2761 ttgcctttgt tttggttttc gctgcccaat accagcactt tattgttgaa actgtcgtcc
     2821 catgtgcgcg agaacggcag ggcatggtcg atttcgacat agcctttttc gttcagacgg
     2881 cctaagttga tttctttgcc cgaatacagg catttgccgt gttgttgctc gtacaggcgc
     2941 agtttcagaa tatctttgga tttgggttcg ccgacaaaat tggggaaata ctctcggaat
     3001 ttggtggcgg ctttttcacg gtctttacgg ttttcttctt ggcgtttttc gatttctttg
     3061 cggtctttaa acgattttcc tacttccctt gccgtttcaa tatggatacg tgcgggggag
     3121 ccgtaacgcc gaaccacgcc gttaatgact tttctggcct gagataaggc acgcaaaaca
     3181 acggggttgc ggatttcgtc ggcagggatg ggtggcagat caatcttttc ttccgtattc
     3241 ttcttgccgt aatggtctcc gtagatttcg gcgcaggctt catcgtaacg tttgccttgt
     3301 tccattagag gcacaattcg gcgcaatgct ttcaaggaaa tttgaacgaa cttgtcgaag
     3361 ctgatgtgtt ttaacagcgc ttctaagatt tcgggctgta cacggtcttt cagacggcct
     3421 gtaatgtcct catcggtttt gaacagggag aatgccgtgc cgatttcgtc ttgtaattcg
     3481 ggagaaaggt ttaatgggga ttttttgtct ttcaatcctt ctttttccag cgcacggctg
     3541 atggcatggt aggccttcat ttccatcaac gttgaggcgt cggcattgtc tttaccatag
     3601 cgcaagcctt tgaaaaaggc ggtattgtct agctccaaaa gcttacgggc ttgtgcgtaa
     3661 gtcagtttgg attttctgta tggctcatct ataagcgtgg tgcgttcggt atcggtcaat
     3721 ggccgctcgc tgccttgctc taaaatacgc aggttattca gtttggttag ccagacgaaa
     3781 cgttcggctg tgtaggtgtt tttagcggct ttcggctctg tcggctcaaa ggtgcaatgc
     3841 cccaacattt tttgaacagc atcgccggat agggcggggc gttgcgtcat cagcagggtt
     3901 tcaatacctt ctttaaggcc gtctgaaata tgcggattgc caaattcttt ttgtttttca
     3961 aacagtaaat tcagctccgc ctgcaaatct ttgcggctga acgtatgcga ataatcgccg
     4021 cgttggttgc ggatatggcc gctttctttc tcaaatttat ttaaagccaa ttcggccggt
     4081 gtgcggaaat cgcctgtctg taaggcatgg gcattgtcgg ctacgccttt aagcaaagcg
     4141 ccaagctcct tatcggcagt ttcgccctcg tttttccgtt gcgataaata gccgcggtgt
     4201 ttgattaaat gcaacaagac tgccgaccac tctaaaggcg tcagtttgcg gtctaatgcg
     4261 gctgcgcgaa gttgccatgg tgtattcggt aaggatttaa tcaagccgtt ttcatcaaaa
     4321 tcggcggctt gtaatacgcc ttcgcgtttc aataggcgac gggcccgaag caggcggtgg
     4381 gcgcgacggc gggtcaggcg gcgaacactg cgtgccaaac gccttgccat ggcgagggag
     4441 tcgcctgttt tcggtacttc tgcacgctca aatacgcgca cgcccaaatc aatcagacgg
     4501 atggggtttt cttcttcgtc aatttctacc atcgcccagc cgacggatgc gatgccgata
     4561 tcgagaccga ggatgtagtt gattggattg ggtttgaagg cagctattta ttactccgta
     4621 aacaacgttg caaataatga taatatatcc gattgtctta tttatataca aatagattat
     4681 tgacttataa tctcacaagg gctagaatcc caaacatatt gtcgcactgc gaaatgagaa
     4741 ccgttgctac aataaggccg tctgaaaaga tgtgccgcaa cgctctgccc cttaaagctt
     4801 ctgctttaag gggcatcgtt tatttcggtt aaaaaatgcc gtctgaaacc ggttttgggc
     4861 ttcagacggc atttgtattt ttggttacca attcacgcgc accgaccggc tgccgagcaa
     4921 tgtttccagt tcgccgaaca atgcggaact cggcgtaacc gtccatttcg gcggcacttg
     4981 aagcctgccc gacgcttttt cgttggcata cgacagttgc agcgggatgc gcggcgtgtc
     5041 gggcagttgg tgggcggcaa gcagttgtac cagtccgccg atgtcgtgat gcggggcgag
     5101 ggcgaggctg aggctgcggg cgtagcgttc gcgtgccgtt tgcagggtca tgacttggtt
     5161 tgccagaata cgcagtccgt cgccgccgcc gtagtcgtcg cggctgactt tggattcgat
     5221 aatcagcact tggtcggctt tgaggcagtc ggcgcagttt tccaacgtct gaccgccgac
     5281 catgatttca acctgtccgc tcaaatcttc aaggctgacg aaggcgattt tgccgcgctt
     5341 gcccatcatg gtgcgtacgg cggtaacgaa tccggcgagg cgcacgctgt cttgcggttt
     5401 gagtttcgac agtttctgcg gggcgatttg gcggacttct tgggcatacg ggccgaacgg
     5461 gtggccggac aggtaaaagc cgatgacggt tttttcttcg gcgagttttt ccgattcgct
     5521 ccacatcggc gcgtcgatga gccgcaccgg ttcgatggcg tcttccatca tgtcgaaaag
     5581 cccgccctga ttggcgttgg cggctttttg gtcggcgttg tccatagcga ggtcgatgtt
     5641 cgccaagagc atggcgcggt tgggttcgat gctgtcgaat gcgccgccgc gtatcagagc
     5701 ttcgagggtg cggcggttca tgtgttcttt gccgacgcgc tcacagaagt ccaagaggcc
     5761 ggtaaacttg ccgccgcttt gccgcgcggc ggtgatggat tcgacggcgg cttcgcccgt
     5821 gcctttaatc gcgccgagcg cgtagcggat tttcatgtcc ggatacggcg tgaagcggta
     5881 gtcggattcg ttgatgtcgg gcggcaggaa ctcgatgccg ttggcacggc agtcgtcgta
     5941 gaaatgcttg agctggtcgg tgttgtccaa ttcggacgac atggtcgccg ccataaattc
     6001 ggcggggtag tgggctttga gccatgcggt ctgataggaa atcagggcgt aggcggcggc
     6061 gtgggatttg ttgaaaccgt agccggcgaa tttttccatg tagttgaaga tttcgtcgga
     6121 tttttcgcgt gaaatgcctt gttttgccgc accttcggcg aagatttcgc ggtgtttcac
     6181 catttcttcg ggctttttct tacccatggc gcggcgcagc aggtccgcac cgccgagcga
     6241 gtagccgccg ataatctgcg ccgcctgcat cacttgttcc tgatacacca taatcccgta
     6301 ggtcggcgcg aggatgcctt cgagtagcgg atggatgtat tggaattctt gccccttcat
     6361 acgcgcgacg aagtcgggga tgttgtccat cgggccgggt cggtagagcg acacgaaggc
     6421 gatgagttct tcaaacttgg tcgtatgcgc cgttttcagc attttcttca tgccggtcga
     6481 ctcgaactgg aatacggcgg tagtgttcgc atcgcggaag atttggtagg caacctggtc
     6541 gtcaagcgga atcttgccga catcgacgat atcgccggtg gtgcttttga tgttgttctg
     6601 cgccatttcg ataatggtca ggttgcgcag gcccaaaaag tcgaatttca ccaaacccac
     6661 atcttccacg tcgcctttat cgtacatgga tacgggcgag gcggattcgt ccgcctgata
     6721 cacggggctg taatcggaaa tcttgcccgg cgcaatcaac acgccgcccg cgtgcatacc
     6781 cagaccgcgc gttaaatctt ccagcttttt cgccaacgta atcagttcgt ccgcttcttc
     6841 cgcttcaatc aattcctgaa tctgcggctc ggtctccatg gctttttcca aactcagggg
     6901 tttgttggct tccaacggaa tcagcttgga cagtttgtcg cacagcataa acggcagttc
     6961 cagcacacgc cccacgtcgc gaatcaccgc tttggacgac attgtgccga aggtaacaat
     7021 ttggctgact gcttccgcgc catatttttc gcgcacatat tcaatcacgc ggccgcgatt
     7081 tgcctgacag aagtccacgt caaagtcggg catggaaacg cgttcagggt ttaagaaacg
     7141 ttcgaacagc agcgcgtatt tgagcgggtc gaggtcggta atcttcagcg aatacgccac
     7201 cagcgaaccc gcgcccgaac cacggcccgg cccgaccgga cagccgtgcg ttttcgccca
     7261 gttgataaag tcttgtacga taaggaaata gccggggaat ttcatctgga tgatgatgtt
     7321 cagctcaaaa tccaaacgtt cctgatattc cggcattttt gccgcacgct ccgcctcgtc
     7381 gggataaagc tgaaccatgc gttcctgcaa accctcgttg gacagtttca ccaaataatc
     7441 atccagtgac aggccgtccg gagtagggaa gagcggcagg aagtttttgc ccaatgtgat
     7501 gtgcaggttg cagcgtttgg cgatttccac cgtgttttcc aaggcctcgg gcaaatcggc
     7561 gaaacgttcg gccatggttt ccggcggaat gaaaaactgg ctcggcgtga aatcgcgcgg
     7621 acgttttttg tccgtcaata cccagccgcc cgcgatgcac acccgcgcct cgtgcgcgtt
     7681 gaaatcgtcg cggctcataa actgcgtcgg atgcgtcgct accaccggca aacccagttc
     7741 ctccgccagc ttcacgctgc ccgaaacgca agcctcccat tcggggcgtt cgggcaggcg
     7801 ttgcagctcc agataaaacg catcgggaaa ccacgccgca tatttcaacg ccgccgcacg
     7861 cgccgcatct tcattgccgt tcaacagatt cacgcccact tcgccgtaat gcgcgccgct
     7921 caaacaaatc aagccgctgt tgtcgccgtt gtccagccat tcgggattga gttccgcgtg
     7981 atggacattg cggtctttgc cgacataagc ctccgtcaaa agctcgctca agcgcagata
     8041 acccgcatcg ttgcggataa tcagcatggc gcgaaacggc ttgtcgggcg cattcggatt
     8101 gcctatccat acgtccgccg cgccgatagg cttaatcccc gcgctgcggc aggctttata
     8161 aaatttcacc aaaccgaatt cgttcatcaa atcgctgatg cccaaagcag gcaaaccgta
     8221 ttcctgcgct ttggcaatca gttttttaat ccgcaccata ccgtcggtaa tcgaaaattc
     8281 ggtatgcagg cgcagcggga tgtaggtcgg ttcggtcatg gcaaaatcgg cgtggacaat
     8341 aaaaggcgta ttgtagcagg gttgtcttta gatggcggtg taggtaatgc tgtttcagat
     8401 tcagacggca tgacctgcaa atgtttttga gcttttacga cggcaaaaaa atgcctccta
     8461 ccgtgtggtg gaggcttccc aaggagtatt gatagatata aaggactatc aaactagtta
     8521 taaggaacta tataccttat tcggacggat ggcaagcagt taaattaatt ttacgttcaa
     8581 acaggttttt gatttcgttt tgatgccgat tgccggtgta tcgggcagtt cgcgtttgag
     8641 gaggtgcgtc atcgtcaatg cggattcgtc ggggaacagg atttgcagcc tgccgccgga
     8701 caggatttct ttgaccggct ggcggcatac ggggtttcag acggcatttt tacggaacag
     8761 gcggtgcgga tacatcgctg gttttgcggt aattttgggt acggcggcgg caggtgctac
     8821 aataacgggc tctttctgca agggaacatt atggaagcca ctgtctatct cgaagacaac
     8881 gaatacatcg ccttgtgcga cctcttgaaa ttggccggac ttgccgaaag cggcggacag
     8941 gcgaaagcgt ttatcgccga agggctggtg tcgcgcaacg gcgaaaccga aacccgtaaa
     9001 accgccaaaa tacgcggcgg cgaagtcatc gagtttgacg gcgcacgctt ggaaatcgca
     9061 gatggatacg accctgaagc ataaagccga agcccttttg ggcgagccgc ttttagacga
     9121 acccgtccgc cccgagtcgt gggaatgctg cggcagcgac tgcggcgagg cgtgcattca
     9181 gacgatttat tgggcagata aggcccgcta cgacgcgcaa cggcggaaat tgaaagaagc
     9241 aggttggtcg gaagacgaaa atgccgtctg aaaacggttc ggctt
//
LOCUS       DAXHSJ010000071         9266 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000071 DAXHSJ010000000
VERSION     DAXHSJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.071"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            165..1508
                     /locus_tag="ACFM8U_001199"
     CDS             165..1508
                     /locus_tag="ACFM8U_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC6334440.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1511..2743
                     /locus_tag="ACFM8U_001200"
     CDS             1511..2743
                     /locus_tag="ACFM8U_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC6334441.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2736..3512
                     /locus_tag="ACFM8U_001201"
     CDS             2736..3512
                     /locus_tag="ACFM8U_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC6334442.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3512..4138
                     /locus_tag="ACFM8U_001202"
     CDS             3512..4138
                     /locus_tag="ACFM8U_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC6334443.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4142..4735
                     /gene="nqrE"
                     /locus_tag="ACFM8U_001203"
     CDS             4142..4735
                     /gene="nqrE"
                     /locus_tag="ACFM8U_001203"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC6334444.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4749..5966
                     /gene="nqrF"
                     /locus_tag="ACFM8U_001204"
     CDS             4749..5966
                     /gene="nqrF"
                     /locus_tag="ACFM8U_001204"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC6334445.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6118..7173
                     /locus_tag="ACFM8U_001205"
     CDS             6118..7173
                     /locus_tag="ACFM8U_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6334446.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPNSEISRFNQTTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTTMSSEFEKLLR"
     gene            7199..7414
                     /gene="nqrM"
                     /locus_tag="ACFM8U_001206"
     CDS             7199..7414
                     /gene="nqrM"
                     /locus_tag="ACFM8U_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC6334447.1"
                     /translation="MKTLLLTFSIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7538..8116)
                     /gene="grpE"
                     /locus_tag="ACFM8U_001207"
     CDS             complement(7538..8116)
                     /gene="grpE"
                     /locus_tag="ACFM8U_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC6334448.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPTYED
                     LQARVADLEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQLTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8300..9118
                     /gene="cysE"
                     /locus_tag="ACFM8U_001208"
     CDS             8300..9118
                     /gene="cysE"
                     /locus_tag="ACFM8U_001208"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC6334449.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKECGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2238 a         2579 c         2297 g         2152 t
ORIGIN      
        1 aaacagcttc cgcaaggaaa aaagcagcaa atctgacaaa caggtacaat tcctgataga
       61 atctaacact ttgctacatt aatttattca atgcttttga tgccacgcga cacggtatca
      121 aaacacgccg tttttacctt aaccatcaca agtaatgcaa atatatgatt aaaatcaaaa
      181 aaggtctaaa cctgcccatc gcgggcagac cggagcaagt catttatgac gggcccgtca
      241 ttaccgaagt cgcgttgctt ggcgaagaat atgccggtat gcgcccctcg atgaaagtca
      301 aggaaggcga tgccgtcaaa aaaggccaag tgctgtttga agacaaaaag aatccgggcg
      361 tggtgtttac cgcgccggtt tcaggcaaaa tcgccgccat ccatcgcggc gaaaagcgcg
      421 tgcttcagtc ggttgtgatt gccgttgaag gcaacgacga aatcgagttc gaacgctacg
      481 cgcccgaagc gttggcaaac ttaagcggcg aagaagtgcg ccgcaatctg attcaatccg
      541 gtttgtggac tgcgctgcgt acccgtccgt tcagcaaaat ccctgccgtc gatgccgagc
      601 cgttcgccat cttcgtcaat gcgatggaca ccaatccgct ggcggcagac cctgtggttg
      661 tgatcaaaga agccgccgag gatttcagac gaggtttgct ggtattgagc cgtttgaccg
      721 agcgtaaaat ccatgtgtgt aaggcagctg gcgcagacgt gccgtctgaa aatgctgcca
      781 acatcgaaac acatgaattc ggcggcccgc atccggccgg tttgagtggc acgcacattc
      841 atttcattga gccggtcggt gcaaacaaaa ccgtttggac catcaattat caagatgtaa
      901 ttgccatcgg acgtttgttt gcaacaggcc gtctgaacac cgagcgcgtg attgctttgg
      961 gtggttctca agtcaacaaa ccacgcctct tgcgtaccgt tttgggtgcg aaagtatcgc
     1021 aaattactgc gggcgaattg gttgacgcag acaaccgcgt gatttccggt tcggtattga
     1081 acggcgcgat tacacaaggc gcgcacgatt atttgggacg ctaccacaat cagatttccg
     1141 ttatcgaaga aggccgcagc aaagagctgt tcggctgggt tgcgccgcag ccggacaaat
     1201 actccatcac gcgtacgacc ctcggccatt tcctgaaaaa caaactcttc aagttcacga
     1261 cagccgtcaa cggtggcgac cgcgccatgg tgccgattgg tacttacgag cgcgtaatgc
     1321 cgctagacat cctgcctacc ctgcttttgc gcgatttaat cgtcggcgat accgacagcg
     1381 cgcaggcatt gggttgcttg gaattggacg aagaagacct cgctttgtgc agcttcgtct
     1441 gcccgggcaa atacgaatac ggcccgctgt tgcgtaaggt gctggaaacc attgagaagg
     1501 aaggctgatt atgggcttga aacattttct ggaaaaaatc gaaccgcact tcctgccggg
     1561 cggcaaacat gaaaaatggt atgccctcta cgaagctgcg gcgacgattt tctatacatc
     1621 cggtgcggta acgcgcaaag cggcacacgt ccgcgatgcg ctcgactcca aacgcatgat
     1681 gattttggtg tggctggctt tgttccctgc catgttctac ggtatgtaca acgtcggcgc
     1741 acaggcattc ggtgcgttaa cgcccgattt gctgcaacaa agcatcgcca acgactggca
     1801 ttacgccctt gccaacgctt tgggcatcaa tatgtcgtct gaagcgggcg tgttgggcaa
     1861 aatgctgttc ggcgcgattt acttcctgcc gatttacgcg accgtattta ttgtcggcgg
     1921 tttctgggaa gttttgttcg ccaccgtgcg caaacatgaa atcaacgaag gtttctttgt
     1981 tacctcgatt ctgtttgcct taatcgttcc gcccacgctg ccgctgtggc aggcagcttt
     2041 gggtatttct ttcggcgtgg tggttgcgaa agaagtattc ggcggtacag gtaaaaactt
     2101 catgaaccct gcgctggcag gccgtgcctt cctgttcttc gcctaccctg ccaacttgag
     2161 cggcgatgcg gtttggacgg cggttgacgg ctattccggc gcaaccgcgc tggcgcaatg
     2221 ggcggcacac ggtgcagacg gcctgaaaaa cgccgtaacc ggtcaaacca tcacttggat
     2281 ggatgcgttt atcggcaaac tgcccggctc catcggcgaa gtatccactt tggcactctt
     2341 aatcggcggc gcgtttatcg tgtttgcccg catcgcttct tggcgcatta ttgccggcgt
     2401 gatgatcggt atgattgcca tgtcttcgct gttcaacttc atcggttcgg acaccaacgc
     2461 tatgtttgct atgccttggt actggcattt ggtcgtcggc ggcttcgcca tcggtatgct
     2521 gtttatggcg accgaccccg tttccgcttc ctttaccaat gtcggcaaat ggtggtacgg
     2581 cgcactgatc ggtgtgatgt gcgtattaat ccgcgtggtc aatccggctt accccgaagg
     2641 catgatgttg gcgattctgt ttgccaacct gtttgccccg attttcgact atttcgtcgc
     2701 acaagcgaac atcaaacgca gaaaggcgcg cagcaatggc taagaaattc gataaagaca
     2761 gcttcagcgg cacgctgatt gtcgtgttgg cggtcagcct gatttgctcg gtcatcgttg
     2821 ccggtgcggt cgtcggcttg aaacccatcc aagagaaaca aaaactccaa gacaaacaag
     2881 gctatatctt gagcgtagcc ggtttgatgg ataaggacac cgacatcggt aaaacctttg
     2941 ccgagcgtat cgagcaacgt gttgtcgatt tggcgaccgg cgaatacgtc gccgatgcgc
     3001 ctaaagactt cagcgcgcgc atcgcaggca aagaccctgc ccaaagcatc cgcatcaaac
     3061 ccgaagacga tttggcaggc atcaaaagcc gtgccaaata caccgaggtt tatttggtaa
     3121 aaggcgaaga cggcaaaatc gggcaaatca tcctgcctat gcacggtaac ggtttgtggt
     3181 cggtcatgta cggctttgtc gccatccagc ccgacggcaa caccatcaac ggcattacct
     3241 actacgaaca aggcgaaacc ccgggcttgg gcggcgaaat cggcaatccg ttgtggcagc
     3301 aaaaattcgt cggcaaaaaa ctgtttgacg gacaaggcaa actcgccctg cacgtcggca
     3361 aaggcgcggg ttcggacaaa gaacacggcg tagatgccct ctccggcgca tcgctgacat
     3421 ccaaaggcgt gcaaggttcg ttcgcctact ggttcggcga aaacggctat atcccctacc
     3481 tgaacaaatt gaaatcagca ggagcgcaat aatggctgat atgaaacgct tgaaacattt
     3541 gatgttttca ccctttatcg acaacaaccc gattgccttg caggttttgg gtatttgttc
     3601 ggctttggcg gttaccacca aacttcagac ggccatcgtg atgggtattt ccgtagcttt
     3661 ggtaaccggt ttttccagct tcttcatttc gctggtacgc aactacatcc ccaacagcat
     3721 ccgtattatc gtgcaaatgg cgattatcgc gtcgctggtt acgctggtcg accaactctt
     3781 gcaggcattt gcctacgaat tgtccaaaca gctttccgta ttcgtcggtc tgattattac
     3841 caactgtatc gtgatgggcc gcgccgaagc atttgcgatg aaagagccgc cgctggaaag
     3901 cctgatcgac ggcatcggca acggcgcggg ctacgggata atgctgcttg tcgtcgccac
     3961 cgtccgcgaa ctgattggct cgggcaaact cttgggctac accgttttcc aaaccgtgca
     4021 ggacggcggc tggtatcaga ccaacggctt gttcctgctc gcccccagcg cgttcttcat
     4081 catcggcttt ttgatttggg gactgcgtac ctggaaaccc gaacaggcgg aggaataagc
     4141 tatggaacac tatttgagcc tcttcatcaa atccgtcttc attgaaaata tggcactatc
     4201 cttctttttg ggtatgtgca cgtttttggc ggtatccaaa aaagtatcca ccgcattcgg
     4261 tttgggtgtg gcggtaattt tcgtactcgg gctgtccgtc cctgtcaacc aactcgttta
     4321 ctcgctgctc aaagacggcg cgattgtcga aggcgtggat ttgacctttt tgaaattcat
     4381 caccttcatc ggcgtgattg ccgctttggt gcagattttg gaaatgttct tggataaatt
     4441 cgtccccgcc ctctataacg cattgggcat ctacctgccg ctgattaccg tgaactgcgc
     4501 gattttcggc gccgtatcgt ttatggcgca acgcgaatac aacttcggcg agtccgttgt
     4561 atacggcttc ggcgcgggct tgggctggat gttggcgatt gtcgctttgg cgggcattac
     4621 cgaaaaaatg aaatattcgg acgctcccaa aggcctcaaa ggcttgggca ttacctttat
     4681 cgccgccggc ctgatggcga tggcgtttat gtcgttctct ggcatccagt tataagaaag
     4741 ggatcggtat ggagattatt ttaggtatcg tgatgtttac cgtcatcgtt ttggttttgg
     4801 cactgatgat tctgtttgcc aaatccaaac tggtcagcga aggcgacatc accatcaaag
     4861 tcaacggcga aaaagagctg acgatgcccg ccggcggcaa actcttgggc gcgcttgcca
     4921 acgaaggcat ctttatcccc tccgcctgcg gcgggggcgg ttcgtgcgga caatgccgcg
     4981 tcgtcgtaaa aagcggcggc ggcgacatcc tgccgaccga gctgtcccac atcagcaaac
     5041 gcgaagcacg cgaaggctgc cgtctgtcgt gccaagtcaa cgtcaaaacc gacatggaca
     5101 tcgaagtccc cgaagaagtg ttcggcgtga aaaaatggga atgcaccgtc atctccaacg
     5161 ataacaaagc cacgttcatt aaagaactca agcttgccat tcccgaaggc gaagaagtcc
     5221 ccttccgcgc cggcggctac attcaaattg aagccccgcc gcacaccgtt gcctacaaag
     5281 acttcgacat tcccgaggaa taccacgaag actgggacaa atacaatttg tggcaatacg
     5341 tttccaaagt ggacgagccg attttgcgtg cctactctat ggcttcgtat cctgaagaaa
     5401 aaggcatcat tatgctgaac gtgcgtatcg ccacgccgcc cccgcgcgta cctgatgcgc
     5461 ctccgggaca aatgtcgtcc tacatctggt cgctcaaacc cggcgacaaa gttacgattt
     5521 ccggcccgtt cggcgaattt ttcgccaaag acaccgatgc cgaaatggta tttatcggcg
     5581 gcggtgcggg tatggctccg atgcgttccc acattttcga ccagttgaaa cgtttgaact
     5641 ccaaacgtaa gattaccttc tggtacggcg cacgttccaa acgcgagatg ttctatgtcg
     5701 aagactttga ccaactcgcg gcagagttcc cgaacttcac gtggcacgtc gccctgtccg
     5761 acccattgcc tgaagacaac tgggacggct acacaggctt cattcacaac gtggtttacg
     5821 aaaaccatct gaaaaaccac gaagcgccgg aagactgcga attttatatg tgtggtcctc
     5881 cgatcatgaa ccagtccgtc atcaaaatgc ttaaagactt aggcgtggaa gacgaaaaca
     5941 tcctcttgga cgatttcggc ggttaaatat tcggcttcaa acacaaaagg aagaaacctc
     6001 ggtttcttcc tttttttatc cgataccgtc cgaacacctt tcagacggca tcggcataga
     6061 aacataccgg ccgttttgcg atataattca aaccatttgc caaaaccgtc aaacactatg
     6121 ccgtctgaaa cacgcctgcc gaactttatc cgcaccttga tatttgccct gagttttatc
     6181 ttcctgaacg cctgttcgga acaaaccgcg caaaccgtta ccctgcaagg tgaaacgatg
     6241 ggcacgacct ataccgtcaa atacctttca aacaatcggg acaaactccc ctcccctgcc
     6301 gaaatacaaa agcgcatcga tgacgcgctt aaagaagtca accggcagat gtccacctat
     6361 cagcctaatt ccgaaatcag ccggttcaac caaaccacgg ccggcaaacc cctccgcatt
     6421 tcaagcgact tcgcacacgt tactgccgaa gctgtccgcc tgaaccgcct gacacacggc
     6481 gcgctggacg taaccgtcgg ccctttggtc aacctttggg gattcgggcc cgacaaatcc
     6541 gttacccgtg aaccgtcgcc ggaacaaatc aaacaggcgg catcttatac gggcatagac
     6601 aaaatcattt tgaaacaagg caaagattac gcttccttga gcaaaaccca ccccaaggcc
     6661 tatttggatt tatcttcgat tgccaaaggc ttcggcgttg ataaagttgc gggcgaactg
     6721 gaaaaatacg gcattcaaaa ttatctggtc gaaatcggcg gcgagttgca cggcaaaggc
     6781 aaaaacgcgc gcggcgaacc gtggcgcatc ggtatcgagc agcccaatat cgtccaaggc
     6841 ggcaatacgc agattatcgt cccgctgaac aaccgttcgc ttgccacttc cggcgattac
     6901 cgtattttcc acgtcgataa aaacggcaaa cgcctctccc atatcatcaa cccgaacaac
     6961 aaacgaccca tcagccacaa cctcgcctcc atcagcgtgg tcgcagacag tgcgatgacg
     7021 gcggacggct tgtccacagg attattcgta ttgggagaaa ccgaagcctt aaagctggca
     7081 gagcgcgaaa aactcgctgt tttcctgatt gtcagggata aaggcggcta ccgcaccacc
     7141 atgtcttccg aatttgaaaa actgctccgc taaacataca aaccgattag gaaacaccat
     7201 gaaaaccctg ctccttacct tcagcatctt cctgaccgtc atcatcggta tggcggtcgg
     7261 ctatattttc tccaaacgca gcatcaaagg cagttgcggc ggcattactg ccttgggtat
     7321 gaaaaaaatg tgcgactgcg acacaccttg cgacaccctg caaaagaaac tggatgaaga
     7381 aaaacaggca ggcggcatac gggttgaccg ttaacgccga aacaaaatgc cgtctgaagc
     7441 ccgacaggcg ggttcagacg gcattgccgc tttacttcga tcggtttata cgcaaaacgc
     7501 gcttcaggaa ataaatcaga ttattcccca gacgccttca ggcttccttc tgcgccaccg
     7561 taaccatagc cgggcgcaac acgcggtcgg acagcgtata acccttcttc atcacgccca
     7621 ccacggtatt gggttcctgt tcgcttgcca ccgcctgcat cgcctgatgg atattcggat
     7681 cgagcttatc gcccgcttta ggattgattt ccttgatttg cgtagcatca aatgctttct
     7741 gcaactcgtt caaagtcagc tgcacgccca ttttcagcgc atcgaaattg ccgctctgat
     7801 ccaaaagcgc catttccaga taatccttga ccggcaacat ttctactgca aacttctgtc
     7861 cggcgaactt gtgtgtatcg gcaatttcct gctgatggcg gcggcgcagg ttctgctcgt
     7921 ttgccaaagc acgcagctgc tcgtctttca actgcgcttc cagatcggca acacgcgcct
     7981 gcaaatcctc ataagtcggc tcggcggcag cctgctcctg cacaccgtcc gcatttccta
     8041 ctgtctcgac ggtttccacc gcctccacat tttcaaccgc ttcttcactg ttttgctgct
     8101 gtgtctgttc gctcatatcg tatcccttaa aacaaattgg aaatcaaaat ccagttatta
     8161 cccgcgcaag ataaggacat tttcaagaaa cttcaagcaa aagcagagaa tttcaaattc
     8221 attttcaaat cccctaccga aaaaataata tagacggtaa gatactgccg accatatcga
     8281 ttttcaaacg tcatcactca tgaaaaaaga ccatttaaac acaaccggtt tcgacctctg
     8341 gcacaccatc cgcgaagaaa ccgcggccgc ggtctccgcc gaaccgatgc tggcaagttt
     8401 tttgcaccaa accgtgttgc gccacgagtc cctcggctcc gtccttgcct accacctttc
     8461 cagcaaactc ggcagcccga ttatggacgt gcgcgcgctg tttgaaatct accagcaggc
     8521 gttgggcagc gacacccaaa tcggcaaatg cgtcgaagca gacttaaaag ccatctacga
     8581 acgcgatccc gcctgcgacg aatattcgct gccgctttta tatttcaaag gcttccacgc
     8641 gattcaggca caccgcatca accatcggct gtatctcgac gggcgcaaaa cgctggcgta
     8701 tttcctgcaa aaccgtatgt ccgaagtatt cggcgtggac atccatcccg ccgcccgttt
     8761 cggatacggg ctgatgctcg accacgccac cggctttgtt gccggcgaaa ccgccgtgtt
     8821 gggcaacaat atttcgattt tgcacggcgt aacgctcggc ggttcgggca aagaatgcgg
     8881 cgaccgccac cctaaaatcg gcgacggcgt gatgatcggc gcaaacgcct cgatattggg
     8941 caatatccgc atcggcagca atgccaaaat cggggcgggc agcgtcgtgg tttcagacgt
     9001 gccgccgtcc atcacggttg tcggcgtacc cgccaaaccc gtggcgcgat cgctcaaaac
     9061 accgtcggcg gatatggatc aaaatatcca gtttaccgaa atcgacttta tgatttgaat
     9121 atccgcacca atgccgtctg aaacgccaaa cgtgcttcag acggcattgc ccgcttcaag
     9181 cctgcgcctt gatatgccgc cgatgccggc acttgccagt cggacaaaat gccttttatt
     9241 cgatacaccc gattcccacg caaccg
//
LOCUS       DAXHSJ010000072         9237 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000072 DAXHSJ010000000
VERSION     DAXHSJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.072"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            88..297
                     /locus_tag="ACFM8U_001209"
     CDS             88..297
                     /locus_tag="ACFM8U_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC6334450.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            386..1600
                     /locus_tag="ACFM8U_001210"
     CDS             386..1600
                     /locus_tag="ACFM8U_001210"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC6334451.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1687..4266)
                     /gene="clpB"
                     /locus_tag="ACFM8U_001211"
     CDS             complement(1687..4266)
                     /gene="clpB"
                     /locus_tag="ACFM8U_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC6334452.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNMPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGTANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLAV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4527..5537
                     /gene="trpS"
                     /locus_tag="ACFM8U_001212"
     CDS             4527..5537
                     /gene="trpS"
                     /locus_tag="ACFM8U_001212"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC6334453.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5543..5884
                     /locus_tag="ACFM8U_001213"
     CDS             5543..5884
                     /locus_tag="ACFM8U_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334454.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPGDLEAVSGKCMETDGKGAPSGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6205..6528
                     /locus_tag="ACFM8U_001214"
     CDS             6205..6528
                     /locus_tag="ACFM8U_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334455.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(6737..8245)
                     /gene="ppx"
                     /locus_tag="ACFM8U_001215"
     CDS             complement(6737..8245)
                     /gene="ppx"
                     /locus_tag="ACFM8U_001215"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC6334456.1"
                     /translation="MTTHPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKIIAKDFQAAISAARNEIQRISKNMRREGWDSAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(8361..9201)
                     /locus_tag="ACFM8U_001216"
                     /pseudo
     CDS             complement(8361..9201)
                     /locus_tag="ACFM8U_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714481.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
BASE COUNT         2137 a         2397 c         2437 g         2266 t
ORIGIN      
        1 atttgaattt ttcagaaaat gaagcggacg tttgggcgga ggcaacttgt ttgataagat
       61 agcaatattt caaaacggag aaagatcatg ccttatgtca atattaaagt aaccggcggc
      121 aaggaagcac cgactgccgc gcaaaaagcg gaactgatcg gcggcgtaac cgaattgctg
      181 gcacgcgtgc tgggcaaaaa tcccgaaaca acggttgtcg tgattgacga agtggatacc
      241 gataactggg gaataggcgg caaaagcgtc agcgaacggc gcaaagaggg caggtaaaaa
      301 gcctgaaaat ctcggtttga tgctttaaat tccgcgtgaa aaagagtaca ttcccaccca
      361 ttgcccaaaa tttacggaac acattatgga taaatttccc aagtctgcaa agctcgatca
      421 cgtctgttac gacatacgcg ggccggttca caaaaaagcc cttcagttgg aagaggaggg
      481 caataaaatc cttaaactca atatcggcaa ccctgcgccg ttcggctttg aagcccctga
      541 tgaaatcttg gtcgatgtca tccgcaacct gccgacttcg caaggctatt gcgattctaa
      601 agggctgtat tccgcccgca aagccattgt tcactactat cagaccaaag gtttgcgcga
      661 tattacggta aacgatgtct atatcggcaa cggcgtgtcc gagctgatta cgatgtctat
      721 gcaggcattg ctcaacgacg gcgacgaaat cctgattccc gcgcccgact accctttgtg
      781 gacggcggcg gcaacgcttg cgggcggtac ggtacgccat tatctgtgcg acgaagaaaa
      841 cggctggttc cccaaccttg ccgatatgga agccaaaatc acgcccaaaa ccaaagccat
      901 cgtcgtcatc aatcccaata atccgacagg cgcggtgtac agcagggaaa tcctgttgga
      961 aatcgccgaa ctggcgcgca agcacggttt gattattttc gccgacgaga tttacgataa
     1021 aatcctttat gacggcgcgg ttcaccacca tattgccgcg cttgcccccg accttttgac
     1081 ggtaacgttc aacggtttgt ccaaagccta ccgcgtcgcc ggattccgcc aaggctggat
     1141 ggtgctcaac gggccgaaac atcatgcaaa aggttacatc gagggtttgg atatgctctc
     1201 gtctatgcgc ctgtgtgcca atacgccgat gcagcacgcg attcagacgg cattgggcgg
     1261 ctatcagagc attaacgaat ttatcctgcc gggcgggcgg cttttggaac agcgcaacag
     1321 ggcgtgggaa ctggtcagcc aaatccccgg cgtgtcctgc gtcaaaccga tgggcgcgat
     1381 gtatatgttc ccgaaaatcg ataccgaaat gtaccgcatc cgcgatgaca tgaaattcgt
     1441 ttacgatttg ctggtgcgcg aaaaagtctt gctggtacag ggaacggggt ttaattggat
     1501 caagcccgac catttccgca ttgtcacgct gccttacgtc catcagattg aagaggcgat
     1561 gggcaggctg gcaagattcc tgcaaaccta ccgccaataa ggggacggtt tgtctgccga
     1621 ggataaaaaa tgccgtctga aacggagatt cccgtttcag acggcatttc caacagcagg
     1681 aacaaattag gcaaatttca gtctgtcgcc gtcggcttcc accctgattt cgctttcggg
     1741 cgcatagttt ccggcaagca gggcttttgc cagcgggttt tcgatttccg actggatggc
     1801 gcgtttgagc ggacgtgcgc cgtaaatcgg gtcgaaaccg gctttggcga tgatgtccag
     1861 tgcggcatcg gaaacagcca ggcgcaggtt ttgtttttcc aaacgttttt ccaagccttt
     1921 gagctggatt ttcgcaatgt tgcggatatt atcctgatcc agtccgtgga acacgaccac
     1981 ttcgtcgata cggttgatca tttcaggacg gaaatgttct ttcacatcct ccatcacaac
     2041 ttctttcacc gcttcgtagt cttgagtacc catttgttgg atatgttggc taccaatatt
     2101 ggaagtcatc acgataacgg tatttttgaa gtccacggtg cgaccttgtc cgtcggtcaa
     2161 gcggccgtca tccaatactt gcagcaggat gttgaacaca tcgggatggg ctttttccac
     2221 ttcgtccagc agaatcacgc tgtacggttt gcggcgcact tgttcggtca ggtagccgcc
     2281 ttcttcgtag ccgacatagc ccggaggcgc gccgattaag cgggcaacgg cgtgtttttc
     2341 catatattcg gacatatcga tgcgaatcag atgatcttcg ctgtcgaaca gaaagcctgc
     2401 cagggcttta cacaactcgg ttttacccac gccggtcggg cccaagaaca ggaagctgcc
     2461 gtaaggcttg ttcggatcgg caagaccgga gcggctgcgg cggatagcgt cggacacggc
     2521 acgcacggct tcgtcctgtc cgaccacgcg gcggtgcaat acttcttcca ttttcagcag
     2581 tttgtcgcgt tcgccttcca tcattttgga tacgggaatg ccggtcatac gggaaaccac
     2641 ctctgcgatt tcctctgcgc cgacattatt acgcaagagt ttgtttgccg gttttgtgct
     2701 gtccgtatct gcccgttcgg cggctgcacg ctgtttttcc aaatgctcca aatcttcata
     2761 catcaatttt gaagccagtg ccaaatcgcc ttgccgtttt gcctgttcga ttttaatttt
     2821 gacttcgtca atttgtttct taatattagc agtaccgtct gaaattgctt tttcggcttt
     2881 ccagatttcg tctaaatcgg cgtattcttt ttgcagaccg ttgatttcct cgtctatcag
     2941 ttccaaacgt tttttgctgg catcgtcttt ttctttttca acgtgcgcct tttccatccg
     3001 aagctgaatt agacggcggt cgattttgtc cattgcttcc ggcttggttt ctttttccat
     3061 cttgacacgg ctggcggctt cgtcaatcaa atcaatcgct ttatcgggca ggaagcggtc
     3121 ggtaatgtag cggtcgctca actccgctgc ggcaacgata gcagggtcgg taatatcgat
     3181 accatggtgg atttcataac gctcctgtaa accgcgcaaa atagcgatgg tgtcttccac
     3241 gcttggctcg ccaaccaata ctttttggaa gcggcgttcg agtgccgcat ctttttcgat
     3301 gtattggcgg tattcgtcca aagtggtcgc gccgatacag tgcaattcgc cacgtgccaa
     3361 agccggtttc agcatattgc ccgcatccat cgcaccgtcg gttttgcctg cgccgaccaa
     3421 agtatggatt tcgtcgatga aaatcagagt gttgccgtcg tctttcgcca aatcgttcaa
     3481 cacgcctttc aagcgttctt caaattcgcc gcggtatttc gcgccggcaa tcaaagccgc
     3541 caagtccaga accaaaaggc gtttgttacg cagggattca ggcacttcgc cgttgacgat
     3601 acgttgcgcc aagccttcaa caatagcggt tttacccaca cccggctcac caatcagcac
     3661 agggttgttt ttggtacggc gttgcaatac ctgaattgca cggcggattt cgtcgtcacg
     3721 accgataacg gggtcgagct taccgtctcg ggcgcgttgg gtcagatcga gcgtgtattt
     3781 tttcagcgca tcgcgttggt cttcggcatt ggcatcgttc acgttttgtc ctcctcgtac
     3841 cgcgtcaatc gcggcattga tgttttgttc ggtcgcgccg gcttctttca aaattttgcc
     3901 ggtcgcatcg ttctgctgca ccaaggcaag caggaaaagt tcactggcga tataggcatc
     3961 accgcgtttg gtggcagcct tgtccatcag gttcaacacc gcctgcaatt cgcggctggg
     4021 cagaatgtcg ccgccctgac cggacacttt cggcaggctg tttaaatgct gctgcaaacg
     4081 ctgtttcact tgcggcatgt tcacgcccgc atgagccaag agcgcggcgg ctccgctgtt
     4141 ttggtcgtca agcagggctt ttaacacaaa gcccgcttcc agatagctgc cgtccgcagc
     4201 caacgccaaa ctctgagctt ctgcaagggc ttgttggaat ttggcggtta atttgtcgta
     4261 tcgcattttt gtttcctttt caaaatgtcc gctgtcgaag cctatatgtg cataattgtg
     4321 gataactcaa gttctgtttt ctgtttttct atatttaatt cgatatatca ttgaatttaa
     4381 agtatataaa aatgtataat aatgtgtata actatatctt cttaatatgg aaaagtctgt
     4441 tgtcggctgg atgtaggtgg caaatcgggt ataatcggcg catctttttc cctttcagac
     4501 ggcattgatg ccgcaaggac atttttatga gcaaaaaacg agttctgacc ggcgtaacca
     4561 ccaccggcat cccgcatttg ggcaactacg tcggcgccat ccgccccgcc gtccgcgcgg
     4621 cgcaaaaccc cgataccgaa tccttcctct tcctcgccga ctaccacggc atcatcaaat
     4681 gccacgagcc ggagatgatt caccaatcca cccaagccgt tgccgccact tggcttgcct
     4741 gcggactcga ccccgagcgc accaccttct accgccaaag cgacattccc gaagtgatgg
     4801 aattgaactg gattctgacc tgcattaccg ccaagggttt gatgaatcgc gcccacgcct
     4861 acaaagccgc cgtgcaggca aatgcggaaa acgggcagga agaccccgat tttggcgtgg
     4921 aaatgggttt gttcagttat ccgattctga tgactgccga catccttatg ttcaacgcca
     4981 acgaagtgcc cgtcggacgc gaccaaatcc aacacgtcga aatggcgcgc gacattgccg
     5041 gacgcttcaa ccaccgtttc caagaactct tcaccctgcc cgaagtgaaa atcgacgaaa
     5101 acgtcgaact cttggtcggt ttggacggac gcaaaatgtc caaatcctac ggcaacacca
     5161 ttccgctttg ggaaaacgac aaaaaaaccc aaaaatcggt caacaaaatc atcaccaata
     5221 tgaaagagcc gggcgagccg aaacagcccg acgaaagccc attgtttgaa atctacaaag
     5281 ccttctccac gccgtctgaa acggcagaat ttacgaaaat gcttgccgat ggcttggctt
     5341 ggggcgaggc aaaaaaactt ttggcggcga aaatcaacgc cgaactcgcc gaaccgcgcg
     5401 aacgctacaa cgagctgacc gccgaccctt cgcaaatcga agagattttg caggcaggcg
     5461 cgcaaaaagc gcgtaaagaa gcacgcgaat tgttggacaa agtacgcgat gcggtcggca
     5521 tccgcccgtt gaaataaacc cgatgccgtc cgaacctctt aaaggcttgc cggaacaaaa
     5581 cgtcgtccgg ctgaccggca agcatcccgg cgacttggaa gccgtcagcg gcaaatgtat
     5641 ggaaaccgac ggaaagggcg caccttcggg ctgggcggca aacggcgtgt gccatacctt
     5701 gtttgccaaa ctggtgggca atatcgccga agacggcggc aaactgacgg attacctgat
     5761 ttcgcattcc gccctgcaac cctatcaggc aggcaaaagc ggctatgccg ccgtgcagaa
     5821 cggacgctat gtgctggaaa tcgacagcga gggggcgttt tatttccgcc gccgccatta
     5881 ttgaggtatt cggacatccc ggaatatatt tgggtttttc aaaccctgca ggaaaaagtc
     5941 cgcaccgtcg ggaaactcaa aaggaggggg atatgtgtta caattttccg aactgttttc
     6001 ataaaatagt tttcggacgt gtttcaatat ggcattgatg ccgccttatt gttcgagaaa
     6061 aaaaccttta tatttaaata taattgggtt ttaactaaat gggaaaccgt tttctctccg
     6121 gccgatgggc aaaatcagcc gattgatgga acacggtccg atttttaagc aaaaccttaa
     6181 tcaacaactc aagaaggata agacatgaaa aaattattga ttgccgcaat gatggcggct
     6241 gccttggcag cttgttcgca agaagccaaa caggaggtta aggaagcggt tcaagccgtt
     6301 gagtccgatg ttaaagacac tgcggcttct gccgccgagt ctgccgcttc tgccgtcgaa
     6361 gaagcgaaag accaagtcaa agatgctgcg gctgatgcaa aggcaagtgc cgaggaagct
     6421 gtaactgaag ccaaagaagc tgtaactgaa gcagctaaag atactttgaa caaagctgcc
     6481 gacgcgactc aggaagcggc agacaaaatg aaagatgccg ccaaataatt tgttgccttg
     6541 gcaaaaatga tgggatgccg cctgccggca acccaaacga aaccgcctga agattttcag
     6601 gcggtttttg agttgtatgc cccttgtttt ttacgcctgc atgaccgttc cgagcagatg
     6661 tgccgcgcat atcgggatgt tgcggacagg caaatgccgt ctggacaggg tttggacgga
     6721 atttgttccg taaggttcag acggcctcga ctttaaacgg catattgatt ttttgccatt
     6781 ggacgctttc atattccaat gcgtcggcaa tcaggggatg gcgttccagc cattccctgt
     6841 caatacgcag gatgaagccg cagctttccg tatccgtgcg caactgcata tttttcggga
     6901 aagacaggtc ttggcgcgaa cggcagaaca gggcagcaag gcgcagggac aaaacggcat
     6961 accacaacat ttcgttggtg ccgatgatgc cgctcatttt tttcatatcg ccgcgatgac
     7021 cgatgaccag ttgggcaagt atggtttgtt ccttgcgtga aaagcccggc atatcggcgt
     7081 tttcgaggat gtaggcagaa tgcttgtgat agccggtgtg ggcgatgtcc aaaccgattt
     7141 cgtgcagcgc ggcggcgcgt ccgagatact gttgccacaa ggcaagctct tgaaccgtaa
     7201 catttttagc gtggcagagg ctgtccataa aggtttgcgc ggtctcggcg gtgcgtttcg
     7261 cctgattgag gctgacgtgg tagcggtgtt ggaactcggc aaccgtttgt ccgcgcatat
     7321 cttcgtttaa accgcgcccg atcaaatcgt aaaacacgcc gtcgcgcagg gcggcttcgg
     7381 ttacggtcat cctgtcgagt ttcatttctt caaacgccgc catcatcacg gcaagtccgc
     7441 cggcaaaaac ttcgatgcgt tccggtttca ggttttcaaa tttggctttt ttgaccgaac
     7501 cggcttcgat gatgcgttcg gcgagggcgc gcattccttt gtaggtaatg tccgcctctt
     7561 ggggcatttc ggcggcaagc acgtcgcgga tggattttgc cgaacccgat gtgccgacgg
     7621 cggaatccca accttcgcgc ctcatatttt tgctgatgcg ctggatttcg ttgcgggcgg
     7681 cggaaatggc ggcttggaag tctttggcga tgattttgtt ttggaagaag cgcaggctgt
     7741 aggttacgca gcccaagggc aggctttcag taatgtcggg attcagcgtc gagccgatga
     7801 caaattctgt cgaaccgccg ccgatgtcga taaccagcat tttgccgccg cccgggggga
     7861 gggtgtggat cacgccggta taaatcagcc gcgcctcttc gcgcccggcg atgatttcga
     7921 tggggaaacc cagtgccgct tcggctttgg gaaggaaatc tgcgatgttt ttggcgacgc
     7981 ggaatgtatt ggttgccacg gcgcgtacct gttcagggcg gaagccgcgc aggcgttcgc
     8041 cgaattttgc cagacagtcc aaagcctgtt cttgggaagc ggcactcagg tttttctgtt
     8101 cgtccagtcc ggcggcgaag cgcaccatct gtttgaacga atcgatgact tttaattgtc
     8161 cgttgttgtt ttcgcaaatc tggaggcgga aactgttgga acccaaatcg acggaggcga
     8221 ggacgtttgc gggatgggtg gtcatggtgg ataccggtgg gggaaaaacg caatgttact
     8281 ctgacggcgc aggcgttgac aataaatgat gcggcggttt ttgattctgc ccacggatgt
     8341 tgccgacggc atttttgcgc ttatttgaaa tccttttcca cgctcatgaa aatctgcgtg
     8401 tttttgcgtg tgtaaaaact tttcatattg ctgtcgattt tcagatagcg gaaattgagt
     8461 tgcggcgtaa agcccttcca agagattttg tcgtgccaca acgacaggtt tgcctgatat
     8521 tcgtggtctt tgcgcgggaa gcggtacaca atggtcccgg gtgcgtcaaa catcctgcgg
     8581 gtatagcgca ggtttgcccg cagacccaag cccgccgtcg aacgttttga ctgcgccgac
     8641 acgcaaaccc ttgcggacgg aagcctgttc cgcctctttc gtcatgttgt gcgaccagtc
     8701 cgcaccgccg taaagcagcc agtctttagg cgcggaatac atcagcgtcg cgcccgccag
     8761 cggcatatgg ctgtcgtatc gggcggcggt gcggtcttcc tgataacgct tccacatatt
     8821 gcccacgttc agcgtcagcc gccagcgttc gctcaagcgt cgggagaaat cggcattgaa
     8881 gccgccgacg aaattgtatc ggctgccgcc taagaggttt tgcccgataa acggcacgat
     8941 gccgaacgag cgcgttaccg aacgattttt atagccgagc gacaggcgca ggcactgttc
     9001 gctgaaatct ttgttatccc aataatgcac gccgccgccg ctgatgccgc cgtagaggaa
     9061 atgatgcccg cccgcattga tttcgcgcga cacgcctaag ccctagcgca aaccgtgtgc
     9121 cttttgcggc aggctgtcgg cggttttcgt ccagcttctg cccgcaaatt caatcgtttt
     9181 tttggacgaa gcgttgttta tagcggatta acaaaaatca ggacaaggcg acgaagc
//
LOCUS       DAXHSJ010000073         9076 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000073 DAXHSJ010000000
VERSION     DAXHSJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.073"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(133..468)
                     /locus_tag="ACFM8U_001217"
     CDS             complement(133..468)
                     /locus_tag="ACFM8U_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6334457.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            597..1502
                     /gene="mtrA"
                     /locus_tag="ACFM8U_001218"
     CDS             597..1502
                     /gene="mtrA"
                     /locus_tag="ACFM8U_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC6334458.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1564..2052)
                     /locus_tag="ACFM8U_001219"
     CDS             complement(1564..2052)
                     /locus_tag="ACFM8U_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6334459.1"
                     /translation="MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2199..3101
                     /locus_tag="ACFM8U_001220"
     CDS             2199..3101
                     /locus_tag="ACFM8U_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6334460.1"
                     /translation="MDTAKKDILGSGWMLVAAVCFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSLSSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3142..4272)
                     /locus_tag="ACFM8U_001221"
     CDS             complement(3142..4272)
                     /locus_tag="ACFM8U_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6334461.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPSAENPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4477..7101
                     /gene="alaS"
                     /locus_tag="ACFM8U_001222"
     CDS             4477..7101
                     /gene="alaS"
                     /locus_tag="ACFM8U_001222"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC6334462.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTTKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7163..7894
                     /locus_tag="ACFM8U_001223"
     CDS             7163..7894
                     /locus_tag="ACFM8U_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334463.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8201..>9076
                     /locus_tag="ACFM8U_001224"
     CDS             8201..>9076
                     /locus_tag="ACFM8U_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HFC6334464.1"
                     /translation="MKTLLQLQTAAQNFAAYYKDQTDERREKDTFWNEFFAIFGIDRK
                     NVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTKAKEQAERYLQEIGRTKPS
                     ALPEYYAVSDFAHFHLYRRVPEEGVENQWQFPLEALPEYITRGVFDFMFGIEAKVRQI
                     QEEADIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFLFFADDSAVFQRNYLFQDF
                     LESCKETDTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTA
                     KQHRALIDCGNFDWRN"
BASE COUNT         2305 a         2399 c         2190 g         2182 t
ORIGIN      
        1 cacgcttacc ccttgtttct caaatgccgt ctgaaaaaaa cggctgtata tgttgtctgc
       61 aaaatatcgg gaagcataac agacaaaatg ccgtctgaaa tattttcaga cggcatttca
      121 atccgattcg gattattttt gcgtttcaac cgcttccaat gcacgcaggg cgtaagtgta
      181 agcggcaccg gcattcaggg cgatggcggt tgccaatgcg ccggcgattt cgctgtcggt
      241 cgcaccggct ttggcggcgg cggcggcgtg aacactgatg cagctctcac aacgtgtggt
      301 aatggcaacg gcgatggcaa tcagttcgcg cgttttggca tcaagtgctt cggcggcggc
      361 ggcctgttcc aatgcgccgt aggcctgcag cattttagga tgcgccttac ccagctcgcc
      421 gaacgatttt ttaaccaatg cggtatgttc tttccaatct ttaaacattt tcttttcctt
      481 tctcttgcgt ttaaccctga tacgcgcttg cgtatctgtt ttcgatgtgc gtattattgc
      541 aattattcag ttgtgtttct cgtttaatca tctcatttta tggttcaaaa agatttatgg
      601 acattctgga caaactggtc gatttcgccc aattgacggg cagtgtggat gtgcagtgcc
      661 ttttgggcgg acaatggtcg gtacggcatg aaaccttgca acgcgaagga ttggtacaca
      721 ttgttacatc gggcagcggc tatctctgca tcgacggcga aacttccccg cgtccggtca
      781 gtacagggga tattgtattt ttcccgcgcg gcttgggtca tgtgttgagc cacgacggaa
      841 aatgcggaga aagtttacaa ccggatatgc ggcagcacgg tgcgtttacg gtcaagcagt
      901 gcggcaacgg acaggatatg agcctgtttt gcgcccgttt ccgctacgac acccacgccg
      961 atttgatgaa cgggctgcct gaaaccgttt ttctgaacat tgcccatccg agtttacagt
     1021 atgtggtttc aatgctgcaa ctggaaagca aaaaaccttt gacggggacg gtttccgtgg
     1081 tcaacgcatt gtcgtccgtc ctgctggtgc ttatcctgcg cgcctatctc gaacaggata
     1141 aggatgtcga actctcgggc gtattgaaag gttggcagga caaacgtttg ggacatttaa
     1201 tccaaaaggt gatagacaaa ccggaagacg aatggaatgt cgacaaaatg gtggcggctg
     1261 ccaatatgtc gcgcgcgcaa ctgatgcgcc gtttcaaaag ccgggtcgga ctcagcccgc
     1321 acgcctttgt gaaccatatc cgcctgcaaa aaggcgcgtt gctgctgaaa aaaaacccgg
     1381 attcggtttt gtcggtcgca ctgtcggtag gctttcagtc ggaaacgcac ttcggcaagg
     1441 cgttcaaacg gcaatatcac gtttcgccgg gtcaataccg gaaagaaggc gggcaaaaat
     1501 aaatcggggc ttcaaacgca aatgccgtct gaaaaggctt tcatacagca tttgcgtacc
     1561 gcgtcatttc aagggctgca tcttcatcac ttccatcaaa aagttggtaa atgcggggtt
     1621 gttgggtttg acatccatat ttttccaacg ctgctgccag ccgcgcaagg cattctggat
     1681 atacagcttg gactgttccg tattgattgc gccccgctgg ctgtctatcg ccgaacgcag
     1741 gtagatttca tacatactgt catcgacggc attgcgtccg accaggcgtt ttctgaagtt
     1801 gttcagatat tgcgccgcct gaaccttggt cattttaccg atacccacct gatagcccaa
     1861 gcgcgtcgct tcatcgctga ttttggcaac atccgtccaa tgcgaagagg caaggcggaa
     1921 accttttgca ggtgcttccg ttttgacggt attgatagga ttcacgggga tttccgtcaa
     1981 tgtgggcaca taaatagact ggcagccgga aagaactgcc gcaatggaaa gagggataag
     2041 gtattttttc atgcccccat tataatcaag tttgccttga gaaaacaaat tgttcggcaa
     2101 gaaaaataaa atttcggcat cagaagcagg caaaaacaca ttccacaagc cttgccgcaa
     2161 ggtttacaat ccgaccgtcc ttatcgcaac gaccgtttat ggataccgca aaaaaagaca
     2221 ttttaggatc gggctggatg ctggtggcgg cggtctgctt caccattatg aacgtattga
     2281 ttaaagaggc atcggcaaaa tttgccctcg gcagcggcga attggtcttt tggcgcatgc
     2341 tgttttcaac cgttgcgctc ggggctgccg ccgtattgcg tcgggacacc ttccgcacgc
     2401 cccattggaa aaaccactta aaccgcagta tggtcgggac gggggcgatg ctgctgctgt
     2461 tctacgcggt aacgcatctg cctttggcca ccggcgttac cctgagttac acctcgtcga
     2521 tttttttggc ggtattttcc ttcctgattt tgaaagaacg gatttccgtt tacacgcagg
     2581 cggtgctgct ccttggtttt gccggcgtgg tattgctgct taatccctcg ttccgcagcg
     2641 gtcaggaaac ggcggcactc gccgggctgg cgggcggcgc gatgtccggc tgggcgtatt
     2701 tgaaagtgcg cgaactgtct ttggcgggcg aacccggctg gcgcgtcgtg ttttaccttt
     2761 ccgtgacagg tgtggcgatg tcgtcggttt gggcgacgct gaccggctgg cacaccctgt
     2821 ccctctcatc ggcggtttat ctgtcgggca tcggcgtatc cgcgctgatt gcccaactgt
     2881 cgatgacgcg cgcctacaaa gtcggcgaca aattcacggt tgcctcgctt tcctatatga
     2941 ccgtcgtttt ttccgccctg tctgccgcat tttttctggg cgaagagctt ttctggcagg
     3001 aaatactcgg tatgtgcatc atcatcctca gcggtatttt gagcagcatc cgccccactg
     3061 ccttcaaaca gcggctgcaa tccctgttcc gccaaagata aaaaatgccg tccgaacatc
     3121 cttcagacgg catatcgggc tttatttccc cgccttcaca tcctgccact ggcgcaccat
     3181 aaacttcaat gccgccggct ggataggcac catgataaag ctgtttttca aatcctcctc
     3241 ggttgggaaa atcgtattgt cgtttttaaa ttcgtcttcc atcagctcac gcgccggctt
     3301 gctcgaaggc gcataagtaa cgaaattgcc gtttttcgcc gacacttccg gatcgaggaa
     3361 gtcgttgatg tatttgtgcg cgttggcgac gtttttcgca tctttcggaa tcacgaaaga
     3421 atccacccaa atccccacgc cctctttggg catcatcacg cggatttttt ccttgccgcc
     3481 cgcttcttcg gcacggcgtt tggcgatgtt caaatcgccg ccgaaaccga ttgttacgca
     3541 ggtatcgccg cgcgccaaat catcgataaa gccggacgaa gtaaagcgtt tgatattggg
     3601 gcggtttttc ttgagtaggg cggttgcctc cctgatgtct tccgtattgc tgctgttcgg
     3661 gtttttaccc aaatagttca acaccatagg atagatttcc gccgcgctgt ccaaatagct
     3721 gatgccgcat tgcttgagtt tggacgtgta ttcggggtcg aacaccaaat cccactggtt
     3781 gtccggcagc ttgtccgtac ccaaagcctt tttcacgcgt tcggtattga tggcgaaggt
     3841 atttgtcccc caataaaacg gcacggcgta ttcgtggccg ggatcgaccc cgtccatcag
     3901 cctcatcatt tcggggttga ggtgtttata attgggaatc agcgacttat cgattttctg
     3961 atacgcacct gccttaatct gcctgcccac aaacgcattg gacggcgcga caatgtcgta
     4021 accggacttg cctgtcagca ccttgctttc cagcgtttca tcgctgtcgt acacatcata
     4081 agtaaccttg atgccgtttt tcttttcaaa atcggcaacg gtttccggat cgacatattc
     4141 cgaccagttg taaattttca atacgttttg gttttccgcc ggtgccggat tttcggcaga
     4201 tggtttgtcc gaaccgccgc acgctgcaag cagcaaagca gtcaggacgg ccaggggcag
     4261 atgtttggtc attatcattc cttgcatatc gggttggaga aagcggccat tatagccgat
     4321 attggcaaca gggcttcaga cggcattcaa aatcccgcca cactcttccg aaaaccgccg
     4381 cttccatagc tagaaacagg gatttgcggt aagataccgc cgttcgtttt ccctgctttt
     4441 accatgacaa gacatttgag agacattgaa aaaattatga aaacctccga actgcgccaa
     4501 aaattcctaa aattttttga aaccaaaggc cacaccgtcg tccgctcttc cagcctcgtg
     4561 ccgcacgacg acccgaccct gctgtttacc aacgcgggca tgaaccaatt taaagacgta
     4621 ttcttaggtt tcgacaaacg cccgtacagc cgcgccacca ccgcgcaaaa atgcgtacgc
     4681 gccggcggca aacacaacga cttggaaaac gtcggctaca ccgcccgcca ccacaccttc
     4741 tttgaaatga tgggcaactt ctccttcggc gactacttca aacgcgacgc catccacttc
     4801 gcttgggaat ttctgacttc ccccgaatgg ctcaacatcc ctaaagacaa actgttggcg
     4861 accgtttacg cggaagacga cgaagcctac aacatctggt tgaacgaaat cggtatgccg
     4921 tccgagcgca tcgtccgcat cggcgacaac aaaggcgcga aatacgcgtc cgacaacttc
     4981 tggcaaatgg gcgacaccgg cccttgcggc ccctgctccg aaattttcta cgaccacggc
     5041 gaagaaatct ggggcggcat tcccggcagc cccgaagaag acggcgaccg ctggatcgaa
     5101 atttggaact gcgtatttat gcagttcaac cgcgacgaac aaggcaatat gaatccgctg
     5161 cccaagccgt ccgtcgatac cggcatgggc ttggaacgca tggccgccgt catgcagcat
     5221 gttcacagca actacgaaat cgacttgttc caagacctgc tcaaagccgt tgcccgcgaa
     5281 accggcgcgc cgttcagtat ggaagaaccc agcctgaaag tcatcgccga ccacatccgc
     5341 tcctgctcat tcctgattgc agacggcgtc ttgccttcca acgaaggccg cggctacgta
     5401 ttgcgccgca ttatccgccg cgccgtgcgc cacggttaca aactgggtca aagcaaaccg
     5461 ttcttccaca aactggtggc cgatttggtc aaagagatgg gcggtgccta ccctgagttg
     5521 aaagagaaac aagcacaaat cgaagaagcg ttgaaaaacg aagaaagccg ttttgcccaa
     5581 acgctggaaa ctggtatggc tttgttggaa aacgcgctgg ccaaaggcgg caaaacactc
     5641 gacggcgaaa tcatcttcaa actctacgac acctacggtt tcccatacga tttgaccgcc
     5701 gacatctgcc gcgaacgcaa tatcgaactg gacgaagcag gcttcgagcg cgaaatggaa
     5761 gcccaacgcg cacgcgcacg cgccgcccaa agcttcaaag ccaacgccca actgccttat
     5821 gacggtcaag acaccgagtt taaaggttat agcgaacgcc aaaccgaatc caaagtcctc
     5881 gccctctaca aagacggcga gcaagtcgac gaattgaacg aaggcgacag cggcgcagtc
     5941 gtcatcgact ttaccccgtt ctatgcagaa tccggcggcc aagtcggcga cgtcggctat
     6001 atcttctcag gcgaaaaccg ctttgaagta cgcgataccc aaaaaatcaa agcggccgta
     6061 ttcggccaat tcggtgtaca aacctcaggc cgtctgaaag tcggcgacag cgttaccacc
     6121 aaagtagatg acgaaatccg caatgccaat atgcgcaacc acagcgcgac ccacctgatg
     6181 cacaaagccc tgcgcgatgt attgggcaga cacgtcgaac aaaaaggctc tttggttacc
     6241 gccgaatcca cccgtttcga catttcccat ccccaagcgg taactgccga agaaatcgcc
     6301 gaagtcgaac gccgcgtcaa cgaagccgtt ttggcgaacg ttgccgtcaa cgccgccatt
     6361 atgagcatgg aagacgcgca aaaaaccggc gccatgatgc tcttcggcga aaaatatggc
     6421 gacgaagtac gcgtattgca aatgggcggt ttctctaccg aattgtgcgg cggcacgcac
     6481 gtttcacgca ccggcgacat cggcctcttc aaaatcatca gcgaaggcgg tattgccgca
     6541 ggcgtgcgcc gtatcgaagc catcaccggc ctgaacgcac tcaaatgggc gcaagagcaa
     6601 gagcgtttgg tgaaagacat cattgctgaa accaaagccc aaaccgaaaa agacgtactg
     6661 gcgaaaatcc aagcaggcgc ggcacacgcc aaagccttgg aaaaagaatt ggcacgcgcc
     6721 aaagccgaac tcgccgtcca cgcaggcgca aaactcttgg acgacgcaaa agacttgggc
     6781 gcagccaaac tcgttgctgc ccaaatcgaa gccgacgcag ccgccctgcg cgaaatcgtt
     6841 accgatttaa ccggtaaatc cgacaacgcc gtgattcttt tagcggcagt aaacgacggc
     6901 aaagtctccc tgtgcgccgg cgtatccaaa ccgttgaccg gcaaagtgaa agcaggcgat
     6961 ctggttaaat ttgcagccga acaagtcggc ggcaaaggcg gcggccgtcc cgacttggcg
     7021 caggccggcg gcacggatgc cggcaaattg cccgaaatgc tggtaagcgt ggaaagctgg
     7081 ctttgtcaga aattaagtta aatttaaatc ttaataagcc atttgagtct tgttcaaatg
     7141 gcttatattt tggattgtta gaatgaatac taaaacagag ttacaaaaac ttttagaaga
     7201 ggatatatca actttaacag aaacactaat ttgtgcagat gctcttcctc caagatatgt
     7261 acgtagcatt gcaactccta ttgtccgtcg atggttaatt gacaaacaat taaatattct
     7321 cgcaaaagaa ataggtttaa ctatagaatt gcccatcctt gatacaagtt tggtatttga
     7381 aaaattgtca accttagaga ataaagttaa tttttatatg gctggtggag tttatttagg
     7441 cggagaattt attagctcta tatatcattc atcacaagaa ttttctggtg aaccaataat
     7501 ctatgctgaa ccaaatataa ttttatgtcc tgctgaaaaa tttttaactt taaaacgagt
     7561 tttccataat ggaaatattt ttaatatgaa tcagataatt acttttttat ccaataaaca
     7621 aggaggagta cattttgata aaaattacga taaatataaa acttggcaag ttgctataga
     7681 aaaagcagca aattttttaa aactcggtaa tccatataat gaagataaat tatctcttag
     7741 tgaagaacat gatactattt tagtagtttt accattagaa aaaggatatg aatggaattg
     7801 tcttgagata gaagtactat ccgctgctca atctctagca aatatctatt gcaacaaagt
     7861 tcgcctcata gatggtcatg tttggaagga ataaaagaaa aagcatagtt ggtaattttt
     7921 agttacgtag ggtgggcttc agcccaccgt tttttcattt ttgattgttg attggtgggc
     7981 taaagcccac cctacggtta ctaaaatccc gtcattacct cgcaggcggg aatccggttt
     8041 gttcggtttc ggttttttag ggacagtgcc gcaacattaa atttccagat tcccactttc
     8101 gcacaaataa cagcgcaaat agggcaaaga taaggatatg ggtttttttc tttgaattta
     8161 ctatacaatt cgcccgtccc cgttatagaa acccaagccg atgaaaaccc tgctccaact
     8221 ccaaaccgcc gcacaaaact tcgccgccta ctacaaagac caaaccgacg aacgccgcga
     8281 gaaagacacc ttctggaacg aatttttcgc cattttcggc atcgaccgca aaaacgtcgc
     8341 ccacttcgaa taccccgtca aagaccctgc cgacaacacc caattcgtcg atatattttg
     8401 ggagggcatc ttccttgccg aacacaaatc cgccaacaaa aacctgacca aggccaaaga
     8461 gcaggcggag cgttatttgc aggaaatcgg gcgcaccaag ccctctgcgc tgcccgaata
     8521 ttacgccgtc agcgattttg cccatttcca cctttatcgc cgcgtacctg aagaaggcgt
     8581 ggaaaaccaa tggcagttcc ctttggaagc attgcccgaa tacatcacgc gcggcgtttt
     8641 cgacttcatg ttcggcatcg aagccaaagt ccgccaaatt caagaagaag ccgatattca
     8701 agcggcggcg gccatcggca ggctgcacga cgcgctcaaa gaagaaggca tttacgaaga
     8761 acacgagctg cgcctcttca tcacgcgcct gcttttcctc ttttttgccg acgacagcgc
     8821 cgttttccag cgcaactatc tctttcaaga ctttttagaa agctgcaaag aaaccgacac
     8881 gctcggcgac aagctcaacc aactctttga attcctcaac acacccgacc aaaagcgcag
     8941 caagacccaa agcgaaaaat ttaaaggttt cgaatacgtc aacggcggcc tgtttaaaga
     9001 acgcctgcgc accttcgact tcaccgccaa gcagcaccgc gccttaatcg actgcggcaa
     9061 tttcgactgg cgcaac
//
LOCUS       DAXHSJ010000074         8885 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000074 DAXHSJ010000000
VERSION     DAXHSJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.074"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            142..571
                     /locus_tag="ACFM8U_001225"
                     /pseudo
     CDS             142..571
                     /locus_tag="ACFM8U_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
     gene            complement(619..1962)
                     /locus_tag="ACFM8U_001226"
     CDS             complement(619..1962)
                     /locus_tag="ACFM8U_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC6334465.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERQLADLKMRGRAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGAWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRNRVHGQVEDAVLNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIRLANDSPFGLGACIYTADTGRA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1967..2620)
                     /locus_tag="ACFM8U_001227"
     CDS             complement(1967..2620)
                     /locus_tag="ACFM8U_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC6334466.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGTTGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2771..3061
                     /gene="gatC"
                     /locus_tag="ACFM8U_001228"
     CDS             2771..3061
                     /gene="gatC"
                     /locus_tag="ACFM8U_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC6334467.1"
                     /translation="MALTLADVDKIAQLSRLHLTAAEKEKSLQELNDIFAMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            3124..4569
                     /gene="gatA"
                     /locus_tag="ACFM8U_001229"
     CDS             3124..4569
                     /gene="gatA"
                     /locus_tag="ACFM8U_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC6334468.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGSSGGGLPIGV
                     QLIGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4566..5498
                     /locus_tag="ACFM8U_001230"
     CDS             4566..5498
                     /locus_tag="ACFM8U_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC6334469.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5541..6971
                     /gene="gatB"
                     /locus_tag="ACFM8U_001231"
     CDS             5541..6971
                     /gene="gatB"
                     /locus_tag="ACFM8U_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC6334470.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVAEKAIKLGLALDAKINQKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQEEFGTRREIKNLNS
                     FRFLEQAINYEVEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPKLPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ASGQGKPTANWMNGELAAALNKAGLELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWEEPEATIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7078..8088
                     /locus_tag="ACFM8U_001232"
     CDS             7078..8088
                     /locus_tag="ACFM8U_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC6334471.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMDGHSEQALSEAEKAQGKILMCCTTAQSDISINIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDNGKPVILLATGTGYAP
                     IRSILLDLIRQNSSRVVHFYWGARHQDDLYALEEAQGFACRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8218..8850)
                     /gene="pdxH"
                     /locus_tag="ACFM8U_001233"
     CDS             complement(8218..8850)
                     /gene="pdxH"
                     /locus_tag="ACFM8U_001233"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC6334472.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
BASE COUNT         2296 a         2738 c         2145 g         1706 t
ORIGIN      
        1 ttttgggaat ctgttttacc ccaatatata aagcaccata ttaaggcgga gtgtcttccc
       61 cactttgacc cgaacccgga aaagacaccg cccaagccaa tcctgatgct gccccgacag
      121 ccaaccatta aggaaatcct aatgaacttt gctttatccg tcattatgtt gaccctcgcc
      181 tctttcctgc ccgtcccgcc tgccggagcc gccgtcttta cttggaagga cggcggcggc
      241 aacagctatt cggatgtacc gaaacagctt catcccgacc aaagccaaat cttaaacctg
      301 cggacgcgcc aaaccaaacc ggcggtcaaa cccgcccaag ccgacgcagg gaagcgcaca
      361 gacggcgcgg cacaggaaaa caatcccgac actgccgaga aaaaccggca gcttgaggaa
      421 gaaaagaatt gccgaaaccg aacggcagaa caaagaagaa aactgccgga tttcaaaaat
      481 gaacctgaag gcggtgggaa actcaaatgc gaaaaacaag gacgatttga ttcggaaata
      541 caataacgcc gtaaacaaat actgccgtta atcggctcta gcgcaaaccc gatgccgtct
      601 gaagcggcac gggtttgttt atttctgcca gtaggttttg acgttgacga actcgtacag
      661 cccgaattcg gacaattcgc gcccgtaacc ggaatcttta acccctccga aaggcaggcg
      721 caaatcgctg ctggtatggc ggttgataaa caccgatccc gcctgtattt tttcggcaaa
      781 ccgccaggcg cgtccggtat cggcggtata aatgcaggca ccgagcccga acggggaatc
      841 attggcaagg cggatggcat gttcttcgtt ttcggcgcgc aaaatcaggg cggccggccc
      901 gaatacttct tctctccaga cgcggcaggc aggatttacc ctgtctaaaa ccgtcgcggg
      961 ataaaaccag cctcgccctt gtgggatttt tccgccggtc aggcacaccg cgccgtttaa
     1021 aacagcatct tcaacctgcc cgtgaacccg gttccgcaaa tcttcacggt gcaacggtgc
     1081 aagcgtggta tcgggatgtt tggggtcgcc cattttcaat ttggcgcatt cggcaagaaa
     1141 cagcgtgata aaacgatcgg ctgcggcttc ggttacgatg atgcgcttgg cggcgttaca
     1201 cgattgcccc gcatcgcgga aacgggaata acaggcttct gcggcggcgc gttccaaatc
     1261 ggcatcgggc atcacgataa aggcgttgct gccgccgagc tccaacacgg ttttcttgag
     1321 gtttgcgccc gcatgtgccg caaggatgcg ccccgtatgc gtcgagccgg taaacgccat
     1381 cgcatcggta tcttcaaccg ccttgagcgt gcccgcctca tccaaccacg cgcctgccaa
     1441 aggaatgccg tctgaagcca aatcgaacaa cgcctgactg acgcgtgcca cgctgggcgc
     1501 gggtttgacg gcgcacgcgt tgcccgcgca catagcggga acggcgaaac gcaatacctg
     1561 ccagacggga tagttccaag gcatgacggc aaacaccacg cccaaaggct caaaccttac
     1621 ctgactcaaa ctcgcctgcg tcgcgatggt tttgtgggcg agcagttcgg gggcaaggcg
     1681 ggcgtaatag cgtatcagtt cgatagactt accgatttcc gcgcggcatt cgtgcaggca
     1741 gcgaccgact tcctcgcaca ccatttccgc aaaacgctct ttctccgcct ccaaacggtc
     1801 ggcaaatttt tgcagacgcg cggcacgttc ggttacgccc aattgcgcga acgccctgcc
     1861 gcgcattttc aaatccgcca gttgccgttc aaactccgca taatcttgag cagggcggcg
     1921 gtaaagcgtt tcgcccgtaa atacattgac actgtgaaac atcgaatcaa cctgccagtt
     1981 gcgggaatat cgttttcagt cccgacacaa taatctccac cgataccgcc gccagcatca
     2041 tacccataat gcggtttaaa atcgtcagcc ccgtcgtgcc cagcaggcgg ctgaccttcc
     2101 cggcaacgat taaaatggca taacaaatcg cactgaccac caaaccggcc gcaataatca
     2161 acgcgatgtc gccgtatgtt ttagccgccg aagcgtaaat aatcacggtc gaaataccgc
     2221 ccgggccgat ggtgatcggt atggcgatgg gcacgacggc aatcgctccg gcattgcggg
     2281 cggggcgcgc ctgccccgtt tccggctgcg cgccgagatt ctgcttggcg ggattgtcgt
     2341 tgccgttcat catagaaatg gcaatcagca acaccaaaat tccgccgccg acctgaaacg
     2401 aaccgacgct gatgcccaaa accttcagca gcgtaccgcc gatcagcgca aataccgcaa
     2461 tcacggcaaa cacggcaacg gcggccgtcc gcgcgacctt cctgcgctcc ttcgtgctgt
     2521 gcccgttggt caggtcaagg taaagcgaca acgcgctaaa cggattaatc agcaccaaaa
     2581 aagccacaat cagcttgccg atttccatac ccaatcccat tatttccccc tccttcaaac
     2641 ccgtgcggca ggcatccgat gctgcaaatt gccgccgcaa cggatttttc cgttataatt
     2701 aaaaattcaa gcaatacgcc ccatcatacc cgaacgacgg tatctttacc atcagacaag
     2761 gatgcttttc atggcactga cacttgccga cgtagacaaa atcgcccaac tctcccgact
     2821 gcacctgact gcggcagaaa aagaaaaatc gcttcaagaa ttaaacgaca ttttcgctat
     2881 ggtcgaacag atgcaaacca ttaacacaga cggtatcgaa ccgatggcgc acccgcacga
     2941 ggccgccctg cgcctgcgcg aagacgaagt aaccgaaacc gaccgcgccg ccgaatacca
     3001 agccgttgcg ccggaagtac gcaaccgcct gtacatcgta ccgcaagtta ttgaagaata
     3061 atccgaatat tcttcagacg gcatcagcaa taccgcccga agccctttaa ggatggaaga
     3121 tttatgaccc aatacacatt gaaacaggca agcgtcctgt tgcagtccaa acagatttcc
     3181 gccgtcgaac tggcaagcgc ataccttgcc gccatcgccg aaaaaaatcc cgccctcaac
     3241 ggctatatca ccatcgacca agataaaacc cttgcagaag cccgtgccgc cgacgaacgt
     3301 atcgcacagg gcaacgcttc ggcacttacc ggcgttcccg tcgcctacaa ggatattttc
     3361 tgccaaaccg gctggcgcag cgcgtgcgct tccaaaatgc tcgacaactt catctccccc
     3421 tacaccgcca ccgtcgtcca aaacctgctc gacgaaggta tggtaacgct cggccgcacc
     3481 aatatggatg agttcgctat gggttcgacc aatgaaaact cattctacgg tgcagccaaa
     3541 aacccatgga atcttgagca cgtccccggc ggttcgtcag gcggttccgc cgccgtcgtt
     3601 gccgcgcgcc tcgcccctgc cgcgctcggt tcggacaccg gcggctctat ccgccaaccc
     3661 gcatcgcact gcggcattac cggcatcaaa cccacatacg gcacggtttc ccgcttcggt
     3721 atggtcgcct acgcctccag cttcgatcaa accggcccga tggcgcaaac tgccgaagac
     3781 tgcgcgattc tgttaaacgc gatggcaggt ttcgacccca aagactccac cagcctcgag
     3841 cgcgaaaaag aagactacac ccgcgatttg aaccaaccgc tcaaaggttt gaaaatcggc
     3901 ctgcccaaag aatatttcgg cgaaggcaac agcgccgatg ttctgacggc attgcaaaac
     3961 accattgatt tgctgaaagc ccaaggcgcg gaattgattg aagtttccct gccgcaaacc
     4021 aagctgtcca tccccgccta ctacgtcctc gcctccgcag aagccagcac caacctttca
     4081 cgttacgacg gcgtacgtta cggacaccgt gccgcccaat tcgccgattt ggaagaaatg
     4141 tacggcaaaa cccgcgccga aggtttcggc agcgaagtca aacgccgcat catgatcggc
     4201 acttatgtac tgtcgcacgg ctactacgat gcctactatc tcaaagccca aaaactgcgc
     4261 cgcctcgttg ccgatgattt tcagacggca tttgcacggt gcgacctcat cctcgcgccg
     4321 accgcaccct ccgccgcacc caaaatcgga gcggatgctt cgccggttga aacctacttg
     4381 agcgatatct acaccatcgc cgtcaacctc gccggactgc ccgcattgac cctgcccgca
     4441 ggctccagcg gcggcggact gcccatcggt gtacagctta tcggcaacta cttcgccgaa
     4501 gccaaaatcc tcggtgcggc gcatcaaatc caactcaaca gcgattggca cggcaaacga
     4561 cccgaatgaa gcagaaccga acctttacct tccccgattt tcgcaccgtt tacagctatg
     4621 cgcctttata tcggctgcaa catttaaaat acacattgcg aaaatttttc ggaaaaaaag
     4681 aaatttacgc cttcgagcag tttgtcaacg cctcccctat ccgtcagggg ctgttcctcc
     4741 actgcccgca aaatgcctat ccgctactgc gcgaatttgt tgacaggcgt tttaactgca
     4801 aacgccgttt agatgcgatg acggcagatt ttctcatggc ggaaaaactg ttcggcacag
     4861 acatcctgca ccaaatggaa gactaccgct tccatttggt cttggcgcac ctttcagacg
     4921 gcatcagctt gtggctcaac cgcaacgaca actgcgtcga agaaggcgcg tggtctttat
     4981 ctttgcgcga cgaagcaggc aaccggctgt atatggcgac cttcgccttt gtcggcacac
     5041 acctgctgac agcctccgta caagggccgg cgggtgaaga agccaaagac accgtccgcc
     5101 gcataaccaa acaactccac ggcttgcgtc cccaacaact gatggtaacc gccctgcaat
     5161 atttcgccgc cgtactcggc ttggacggcg caatgggcat tgcacaaaaa catcaggtca
     5221 aactgcgctg gaaacttaaa aagcgtgtca aaatgaatta cgacgcattc tggcaggaat
     5281 acggcgcaag tttggaacgg gacggctact ggcatctccc ccaaaccccc gcccgcaaag
     5341 accttaccga catcgaaagc aaaaaacgct cgatgtaccg caagcgttat gaaatgctgg
     5401 acaacatggt tgcagagatg aaggacggtc tgaaaacaga agcacgcggc atttcagacg
     5461 gcatccaaac ggaaaaaccg ctccgccgga cagcctgacg cgaagactat cgaattgata
     5521 ttttagagaa agaagctctt atgacctggg aaaccgtaat cggcttggaa atccacgtcc
     5581 aattgaacac caaatccaaa atcttcagcg gcgcatcgac cgcattcggc gcagaaccca
     5641 acgcgcacgc cagcgtggtg gaatgcgcgc tgccgggcgt actgccggta atgaaccgtg
     5701 aagtcgctga aaaagccatc aaattgggtt tggctttaga tgcgaaaatc aatcagaaaa
     5761 acgtgttcga ccgcaaaaac tacttctatc ccgacttgcc caaaggctat caaatcagcc
     5821 agttggactt accgattgtc gaacacggca aattggaaat cgtggtcggc gacgatgtga
     5881 aaaccatcaa cgtaacccgc gcccatatgg aagaagacgc gggcaaatcc gtgcatgaag
     5941 gcttaaacgg cgcgaccggt atcgacctga accgcgcagg cacaccgctg ttggaagtcg
     6001 tatccgaacc cgaaatgcgc tccgccgccg aagccgttgc ctacgccaaa gccctgcaca
     6061 gtttggtaac ctggctggac atttgcgacg gcaatatggc ggaaggttcg ttccgcgtcg
     6121 atgccaacgt atccgtgcgc ccgaaaggtc aagaagagtt cggcacgcgc cgcgagatta
     6181 aaaacctcaa ttccttccgc ttcttggagc aggcgattaa ttacgaagtg gaagcgcaaa
     6241 tcgagatttt ggaagacggc ggcaaagtac agcaggcaac catgctattc gaccctgaaa
     6301 aaggcgaaac ccgcgtgatg cgcctgaaag aagacgcgca cgactaccgc tacttccccg
     6361 atcccgattt gctgcccgtc atcatttcag atgcccaaat gcaaaaagcc aaagcagaaa
     6421 tgcccaagct gccgaaagaa atggcggcgc gtttcgtggc ggattacggc gtgtccgaat
     6481 acgacgcgcg cctgctgacc gcaagccgcg cacaggccgc ctattttgaa gaagccgcca
     6541 aagcaagcgg acaaggcaag ccgactgcca actggatgaa cggcgaactc gccgccgcgc
     6601 tgaacaaagc cggcctggaa cttgccgaca gcccgattac cgccccgcgc ctcgccgcgc
     6661 tggtgggcaa aatcgccgat ggcacattaa gcagcaaact ggcgaaaaaa gcctttgaag
     6721 ccatgtggga agaacccgaa gccaccattg ccgaaatcat tgaaaaacac ggtttgcaac
     6781 agatgaccga taccggcgcg attgaagcca tggtggacga agtgctggcc aacaacgcca
     6841 aagccgtgga acagtttaaa tccggcaacg aaaaagccct gaatgcgatt gtgggacaag
     6901 tgatgaaggc cagcaaaggc aaagcgaatc ccgcgcaggt gcaggagttg attaaagcta
     6961 aactggctta atccgttatc acacaggtcg tctgaaagca aagttccaac gaaggtaaaa
     7021 caggaaataa gctttcagac ggccttttaa cttaatctgc tcaaaccata ccaagacatg
     7081 aaccacaccg ttaccctgcc cgaccaaacc acctttgccg ccaacgacgg tgaaaccgtt
     7141 ttggccgctg ccgcccgaca aaacctcaac ctgccccatt cctgcaaaag cggtgtctgc
     7201 ggacaatgca aagccgaact ggtcagcggc gatattcaaa tggacggaca ctcggaacag
     7261 gctttatccg aagcagaaaa agcgcaaggc aagattttga tgtgctgcac cactgcgcaa
     7321 agcgatatca gcatcaacat ccccggctac aaagccgatg ccctacccgt ccgcaccctg
     7381 cccgcacgca tcgaaagtat tattttcaaa cacgatgtcg ccctcctgaa acttgccctg
     7441 cccaaagccc cgccgtttgc cttctacgct gggcaataca ttgatttact gctgccaggc
     7501 aacgtcagcc gcagctactc catcgccaat tcgcccgacc aagaaggcat tttggaacta
     7561 cacatccgca ggcgcgaaaa cggtgtctgc tcggaaatga ttttcggcag cgaacccaaa
     7621 gtcaaagaaa aaggcatcgt ccgcgttaaa ggcccgctcg gttcgtttac cttacaggaa
     7681 gacaacggca agcccgtcat cctgctggca accggcacag gctacgcccc catccgcagc
     7741 atcctgctcg accttatccg ccaaaacagc agccgcgtcg tccatttcta ctggggcgcg
     7801 cgtcatcagg atgatttgta tgccctcgaa gaagcacaag gattcgcatg ccgtctgaaa
     7861 aacgcctgct tcacccccgt attgtcccgc cccagagagt gctggcaagg aagaaaaggc
     7921 cacgtacaag acatcgcggc acaagaccac cccgacctgt cggaatacga agtatttgcc
     7981 tgcggctctc cggccatgac cgaacaagca aaaaatctgt ttgtgcaaca gcataagctg
     8041 ccggaaaact tatttttctc cgacgcattc acgccgtccg catcataatt ccccggtata
     8101 aaaaagattc aggctttccg ttcggaacac aaaaaacttc ccgtccgtat tttccccgtg
     8161 aaaaaatgcc gtctgaaacc cgattcctgt tttcagacgg catatgtttt ttcctgttca
     8221 aggcgacagc cgctcgcgta tccagccgcc atccagcaaa cggtattgga tgcggtcgtg
     8281 cagcctgctc ggcctgccct gccaaaactc aatcaaatcg ggaatcacaa tatagccgcc
     8341 ccaatgcggc ggacgcggca cgtgcaaagg atgtttgagt ccgaccgccg ccgcctttgc
     8401 caccaatacc gccttgttcg gaatcacctc gctctgcgca cttgcccacg cacccaaacg
     8461 gctctgatac gggcggctct caaaatattc gtccgacaac ttctccgcca gcctttcaac
     8521 acgcccttcc acgcgcacct gacgctccag ctccggccaa aaaaacgtca tcgccgcaaa
     8581 tggatgagca tccagcgaac gccccttgcg gctgtgataa ttcgtaaaga aaacaaaacc
     8641 ttcagaatta acttccttca gcagcaccat acggctgtta ggcctgccgc gtccgtcgac
     8701 cgccgccaca ttgaccgccg tcggctcgtt gacctgtgcg cgtaccgcct cgtccaacca
     8761 ccgctcgaac tgctcgatcg gattgtcggc gcaatcgcct tccgacaatt cccgtttgct
     8821 gtaatcttcc cgaatattgt gcaaatccat ttaccgctcc tcttaatagt ggattaacaa
     8881 aaacc
//
LOCUS       DAXHSJ010000075         8857 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000075 DAXHSJ010000000
VERSION     DAXHSJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.075"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..1939
                     /locus_tag="ACFM8U_001234"
     CDS             <1..1939
                     /locus_tag="ACFM8U_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC6334473.1"
                     /translation="ISPEIFGTLFQSVMDAQERREAGAHYTETANIDKVINGLFLENL
                     RAEFEAVKALKRDKDKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAE
                     ALKDKTGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEV
                     ACHTLPLEDAAEIIHANSLRTPWQAADYIFGDPPFIGSTYQTKEQKNDLESICGHIKG
                     YGLLDYVCNWYVKAAGIMAQHPQVQTAFVSTNSICQGQQVEILWGSLLKQDIEIHFAH
                     RTFQWTSQAAGKAAVHCIIVGFRQKPQMPSEKTLYDYPDIKGEPVKHAAANINPYLID
                     APDVIIAKRSRPVHCEPDMVNGSKPTEGGNLILSTDEKEALIAAEPLAEQYIRPFVGA
                     EEFINGKTRWCLWFHGVSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATP
                     WLFQKIRQPSDGNFLIIPRVSSESRRFVPIGYLSYETVVSDLVFTLPNASLYHFGILS
                     STMHNAFMRTVAGRLESRYRYSNTVVYNNFPFPESCRMPSENDRQDPLRAAVETAAQA
                     VLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALDKAVDKAYGYKTGKNT
                     DDEAERTAFLFELYRKAAAIA"
     gene            complement(2464..3147)
                     /locus_tag="ACFM8U_001235"
     CDS             complement(2464..3147)
                     /locus_tag="ACFM8U_001235"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC6334474.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            3443..5746
                     /gene="parC"
                     /locus_tag="ACFM8U_001236"
     CDS             3443..5746
                     /gene="parC"
                     /locus_tag="ACFM8U_001236"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC6334475.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPIAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            5766..7342
                     /locus_tag="ACFM8U_001237"
                     /pseudo
     CDS             5766..7342
                     /locus_tag="ACFM8U_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
     gene            7335..7802
                     /locus_tag="ACFM8U_001238"
     CDS             7335..7802
                     /locus_tag="ACFM8U_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFC6334476.1"
                     /translation="MTELQHPVLVVDNETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            7906..8658
                     /locus_tag="ACFM8U_001239"
     CDS             7906..8658
                     /locus_tag="ACFM8U_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6334477.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLTEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAKPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         2205 a         2774 c         2125 g         1753 t
ORIGIN      
        1 catcagtccc gaaatcttcg gcacgctctt ccaatcagtc atggacgcac aagagcggcg
       61 cgaagcgggc gcacactaca ccgaaaccgc caacatcgac aaagtcatca acggcctttt
      121 tttagaaaac ctgcgcgccg aatttgaagc cgtcaaagcc ctcaaacgcg acaaagacaa
      181 aaaactcgcc gccttctacc aaaaaatcca aaacctgcaa ttcctcgacc cggcctgcgg
      241 ctgcggcaac ttccttatcg tcgcctacga ccgtatccgc gccctcgaag acgacatcat
      301 cgccgaagcg ctcaaagaca aaacaggcgg cttattcgac agcccgtccg tccaatgccg
      361 tctgaaacaa tttcacggca tcgaaataga cgaatttgcc gtcctcatcg cccgcaccgc
      421 catgtggctc aaaaaccacc aatgcaacat ccgcacgcaa atccgcttcg acggcgaagt
      481 cgcctgccat acgctgccgc tcgaagacgc cgccgaaatc atccacgcca acagcctccg
      541 cacaccttgg caggcggcgg actacatctt cggcgatccg ccctttatcg gctcgaccta
      601 ccaaaccaaa gagcagaaaa acgacctcga aagcatctgc ggccatatca aaggctacgg
      661 cctgttggat tacgtctgca actggtatgt caaagccgcc ggtatcatgg cgcagcatcc
      721 ccaagttcag acggcatttg tttccaccaa ttccatctgc caaggccagc aggtcgaaat
      781 cctttggggc agccttttga agcaagacat cgaaatccac tttgcccacc gcacctttca
      841 atggacgagc caagccgcag gcaaagccgc cgtccactgc atcatcgtcg gcttccgcca
      901 aaagccgcaa atgccgtctg aaaaaaccct ctacgactat cccgacatca aaggcgaacc
      961 cgtaaaacac gccgcggcca acatcaatcc gtatctgatt gacgcgcccg atgtgattat
     1021 tgccaagcgc agccgtcccg tacattgcga acctgacatg gtcaacggaa gcaaaccgac
     1081 cgaaggcggc aaccttatcc tttcaaccga tgaaaaagaa gccctgattg ccgccgaacc
     1141 cttggcggaa caatacatcc gtccctttgt cggcgcagag gaatttatca acggcaaaac
     1201 ccgttggtgc ctgtggtttc acggcgtatc cgacgttaaa cgcaaccacg acctgaaaca
     1261 gatgccccaa gttcaagccc gtattcaggc agtcaaaacc atgcgcgaag ccagcagcga
     1321 caaacaaact caaaaagatg cagcaacccc gtggcttttt caaaaaatcc gccagccttc
     1381 agacggcaat tttctgatta tcccacgcgt ctcatccgaa agccgccgtt tcgtccccat
     1441 cggttatcta tcatatgaaa cagttgtcag cgatttggta tttacccttc caaacgccag
     1501 cctctaccac ttcggcatcc tcagctccac catgcacaac gcctttatgc gtaccgttgc
     1561 cggccgtctg gaaagtcgtt accgctattc caataccgtt gtgtacaaca acttcccctt
     1621 ccccgaaagc tgccggatgc cgtctgaaaa cgaccgccaa gacccgctcc gcgccgccgt
     1681 cgaaaccgcc gcccaagccg tcctcgacgc gcgcggacaa taccgccgag aagcgcggga
     1741 agccggtttg cccgagccga ccctcgccga actctatgcg cccgacgcag gctataccgc
     1801 cctagacaaa gcccacgccg ccctcgacaa ggcagtcgat aaagcctacg gctacaaaac
     1861 aggcaaaaac accgacgacg aagccgaacg caccgccttc ctgttcgagc tgtaccgcaa
     1921 ggcggcggca attgcgtagt agttgcgtag tagttgcgta gtagttgcgt aggatagttg
     1981 cgtagggtgg gcttcagccc accaactccc accaactccc accaactccc accaactccc
     2041 accaactccc accatcttca ccctaaaacc ccgtcatctc cgcaaaagca attgcgtagg
     2101 gtgggcttca gcccaccgtt tttcttcatt ttcggttgtt gattggcagg ctgaagccca
     2161 ccctgcggtt acaaaaatcc cgtcattccc gcgcaggcgg gaatccagaa cgcgggacgg
     2221 cggcaatatt caaaggttgt ctgaaaattc agaggttcta gattcccgcc tgcgcgggaa
     2281 tgacggcgga gcggtttctg cttttccgat aaatgaccgc aacctaaaac ccatccttcc
     2341 cacgaaaaca gaaaaacaaa aacctaaaat cccgtcgttc tcgcgaaagc agttgctacg
     2401 tttaccgtca aaaatgccgt ctgaacgctc aagccttcag acggcatttt ttatttcaca
     2461 cccttacagg tagaattttt cgatgacttt caaattgtcg tccaatttgt acaccaacgg
     2521 ctgaccggtc gggatttcca agcccataat gtcttcgtcg gaaatgccct cgatgtgttt
     2581 tgccagcgcg cgcagggagt tgccgtgcgc cgccaccaag acgcgtttgc cgctcaaaat
     2641 cgcgggggcg atttggtctt cccaaaacgg caatacgcgc tccagcgtta cttgcaggtt
     2701 ttcgccgtcg ggtacgacat cggcaggcag atgggcatag cggcggtctt tgtgtgcgga
     2761 aaactcatcg tctttgtcca aaagcggcgg cagggtgtcg tagctgcgcc gccagatgcg
     2821 gacttgctcg tcgccgtatt gttcggcggt ttgttttttg tccaggcctt gcagtcggcc
     2881 gtagtggcgt tcgttcagcc gccatgtttt gatttgcggt acgaacagtt ggtcggattc
     2941 ttccaaaacg atgttgcagg tcttaatcgc gcgggtcagg acggatgtga aggcgatgtc
     3001 gaactcatag ccgttttctt tcagtttttt accggcggca gcagcctcgg caagcccctg
     3061 ctcgctcagc ttcacgtcgc gccagcctgt aaacaggttt ttcgcgttcc attcgctttg
     3121 tccgtggcgg ataaatacca attccatatc gtctccaatg tgtgaaagtg ggaaagcctt
     3181 atttataaca tattttcaca ttcccgtatt tgattcagat tcagacacgc gcccactatg
     3241 gtttgccgtt ttgatttaca ataatgccct ttgctttaca ttccgcatac acaatgtttc
     3301 aacacacagg acgacacata aagcaccgcc ctatgtgttg ccctgatttg gaaggggttg
     3361 ccccctccca aataaagtct gattctaccg ccccgaagga cagatgtcca agtggcgggg
     3421 gttcaaccga aaaggaaata cgataaatat gcaaccgcac gctccacata ccgattccaa
     3481 tacgctgatg ctcggccgat acgccgaacg cgcctatctc gaatacgcca tgagcgtggt
     3541 caaaggccgc gcgctgcctg aagtttcaga cggccaaaag cccgttcagc ggcgcatttt
     3601 gtttgccatg cgcgatatgg gtttgacggc gggggcgaag ccggtgaaat cggcgcgcgt
     3661 ggtcggcgag attttgggta aataccaccc gcacggcgac agttccgcct atgaggcgat
     3721 ggtgcggatg gcgcaggatt ttaccttgcg ctatccctta atcgacggca tcggcaactt
     3781 cggttcgcgc gacggcgacg gtgcggcggc gatgcgttac accgaagctc ggctgacgcc
     3841 gattgcggaa ttgctgttgt ccgaaatcaa tcagggaacg gtggatttcg tgccgaacta
     3901 cgacggcgcg tttgacgagc cgctgcacct gcccgcccgc ctgcctatgg tgttgctcaa
     3961 cggcgcgtca ggcatcgcgg tgggcatggc gaccgagatt ccgtcgcaca atttgaacga
     4021 agtgacgcag gcagcgattg cgctgttgaa gaagccgacg ctggaaaccg ccgacctgat
     4081 gcaatatatt cctgcgcccg attttgccgg cggcggtcaa atcatcacgc cggcggacga
     4141 attgcgccgg atttacgaaa ccggcaaagg cagcgtgcgc gtgcgtgcgc gttatgaaat
     4201 cgagaagctg gcgcgcggac agtggcgcgt catcgtaacc gagctgccgc cgaacgccaa
     4261 ttccgccaaa atccttgccg aaatcgaaga acaaaccaac ccgaaaccga aagcgggtaa
     4321 gaaacagctc aaccaagacc agctcaacac caaaaagctg atgctggatt taatcgaccg
     4381 cgtgcgcgac gagtccgacg gcgaacatcc cgtgcgtctg gtgttcgaac cgaaatccag
     4441 ccgcatcgat accgatacct tcatcaacac gctgatggcg caaacttcgc tggaaggcaa
     4501 tgtgtccatg aacttggtga tgatggggct ggacaaccgc cccgcgcaga aaaacctgaa
     4561 aacgattttg caggaatggc tggatttccg caccgtaacc gtaacacgcc gtctgaaatt
     4621 ccgtttgaac caagtggaaa aacggctgca catcctcgaa ggccgtctga aagtctttct
     4681 gcacatcgac gaagtgatta aagtcatccg cgaatcagat gacccgaaaa ccgatttgat
     4741 ggcggcgttc gggctgaccg aaatccaagc cgaagacatt ttggaaatcc gcctgcgtca
     4801 gttggcgcgt ttggaaggtt tcaaactcga aaaagaattg aacgaattgc gcgaagaaca
     4861 aggccgtctg aacatccttt tgggcgacga aaacgaaaaa cgcaagctga ttatcaaaga
     4921 aatgcaggcg gacatgaaac agttcggcga cgcgcgccgc acgctggtgg aagaagccgg
     4981 acgcgccgtg ctgacacaaa ccaccgccga cgaacccatc acactgattc tgtctgaaaa
     5041 aggttggata cgcagccgcg ccggacacaa tctcgatttg agccaaaccg cgttcaaaga
     5101 aggcgaccgc ctcaaacaaa ccctcgaagg cagaacggtt ttacccgtcg tcattttgga
     5161 ttcatcgggc agaacctaca cccttgatgc cgccgaaatc cccggcgggc gcggcgacgg
     5221 cgtgccggtt tcatccttga tcgagctgca aaacggtgcg aaacccattg cgatgttgac
     5281 aggattgccg gaacaacatt atttattatc aagcagcggc ggctacggct tcatcgccaa
     5341 actgggcgat atggtcgggc gcgtgaaagc gggcaaagtg gtgatgaccg cagacagcgg
     5401 cgaaaccgtt ttgccgccgg ttgccgtcta tgcctcctcg ttcatcaacc ccgactgcaa
     5461 aatcattgcc gccaccaatc aaaaccgcgc cctcgccttc cccatcggcg aattgaaaat
     5521 tatggcgaaa ggcaaagggc tgcaaatcat cggattaaac gccggcgaat cgatgacgca
     5581 taccgccgtt tcttccgagc cggaaatcct gattgaaagc gaaggcaggc gcggcgcagc
     5641 gcacaaagac cgcatcccca tctccctgct tgaggcaaaa cgcggcaaaa aaggcaaact
     5701 attacccata tcgggcagcc tgaaacagct ttcttccccg aaataaaccc gactctgcac
     5761 atactatggt aatttccaat acccgcgaac ttgagaaact caaagaccgg attcccaatc
     5821 tgatcaacat cgcccgcatc gccatcgtct tccccctgct gattatgcat atcttcgggt
     5881 tggaggtcag cggacatgaa aacctgcacg cttcgtggac ggcgtgggca ttttatgttt
     5941 ggctcgccat ttcctgctgg ctgattttct tttccatcat ccatccgcat tggcaatggc
     6001 agtcgctgaa aatgccgcgt ttcagcgcgg tggcggacat cacgatgatc ggcgtgctga
     6061 cctacctgtt cggcggcatc gattccggct tcggcatcct gattctgccc ttcgtcgtct
     6121 gctcctgcct gctcagctac gggcgttacc ccctgctcta tgccagctac gccgccatcc
     6181 tgctgatatt gaatgccctt gcagacagca atatcaatat gtatccgctg attctggatg
     6241 caaaaaccgt caccaacacc ttcgtcctcg tggctggttc ctatttcgtc gccatgattg
     6301 cctcactgtc ggtcagatac atcgaccgtg ccggaaaact cgcctacgac agccatatcg
     6361 cctaccggcg catcaaaggc ttgagccaaa ccgtactcaa ccgcgttcag gaagcggtcg
     6421 tcgtcatcaa cgtcgagcat cagaccatac tgttcaataa aaaggcaaaa gacctgctac
     6481 ccatgcttga aatcggacag catactgccc tgttcgaccc tattaccgtt ttatgggaca
     6541 aggcctcttc gcgtactttc gaacgcaata tcgacacgcc cggcctgaac gccggcatcc
     6601 gcgccgttcc ggtcaacaaa gggccggaca agctgctcat cctctacatc cgcccgcaaa
     6661 gcgaaattca ggcagaagcc ctgtccgtca aacttgccgc gctcggacaa ctgaccgcca
     6721 acctcgccca cgaaatccgc aacccgatgt ccgccatccg ccacgccaac gacctgctgc
     6781 gcgaaaatat ggaagcgggg gcggcagatc cgttcaacgc caaattgtgc aaaatcatcg
     6841 acggcaacgt ctgccgtatc gacaaaatgc tcgaagacat ttcctcgctc aacaagcgca
     6901 acaaaaccga acgcgaaacc atcagcctga taccgttttg ggaagaattc aaacaagagt
     6961 tcctgctcgg ccatcccgat gccgccggct gcatccgtcc gaacattcag ggcggcagcc
     7021 tgaccgccta tttcgatccc gcccacctgc gacaaattat gtggaacctc gccaacaacg
     7081 cgtggcgaca cagccgcaaa cagcccggct caatttccgt caccatccgc cccgcgcaaa
     7141 aaaacaccgt ctgcatcctc tttgccgacc gcccgaagtg caggaacacc tgttcgaacc
     7201 cttttacacc acggcggaaa acggcaccgg cctcgggctg tatgtcgccc gcgaactggc
     7261 gcacgccaat ttcggcgatt tgacctacct gcccgaagcc aaatgtttcg aactcgcatt
     7321 accggaaaaa accaatgact gaactgcaac accccgtcct cgtcgtcgat aacgaaaccg
     7381 acattctcga cctgatggaa atgaccctga tgaaaatggg cttgcgcgtc cataccgcgt
     7441 caggcgttgc cgaagccaaa aacaagctcg acagccaacg ctattcgctc gtcctgaccg
     7501 atatgcgtat gccggacggc tcggggctgg aagtcgtcca acacatcaac agccgcctgc
     7561 tcgatacgcc ggttgccgtc atcaccgcct tcggcaacgc cgatcaggca caggaagcgt
     7621 tgcgttgcgg cgcgttcgac cccgatacca tgcagataca ggactatctc gaccaaatcg
     7681 aacgcgacat tatcgaacaa accctcaaac aaaccgaagg caaccgcacg caggccgcca
     7741 aacgcttggg catcagcttc cgttccatgc gctaccgtat ggaacgcctc aacatcggct
     7801 gacgacaaaa cggcatccgc accatctccg cccacccgaa aaaatgccgt ctgaaacggc
     7861 acgggaaagc gggttcgccc cacgcccgaa cggacacaaa acaccatgac cgacatcctt
     7921 attgacaaca ccgccaccga aaccgtccgc accctgatac gggcattccc ccttgtgccc
     7981 gtttcccaac cgcccgaaca aggcagctac ctccttaccg aacacgatac cgtcagcctc
     8041 aggcttgtcg gggaaaaaag cagcgtcatc gtcgactttg cctcgggagc cgcacaatac
     8101 cggcgcacaa aaggcggagg agaactcatc gccaaagccg tcaaccatac cgccaaaccc
     8161 accgtatggg atgcaaccgc aggattgggg cgcgacagct tcgtcctcgc ctcgctcgga
     8221 ctgaccgtta ccgccttcga gcagcatccc gccgtcgcct gcctgctttc agacggcatc
     8281 cgccgcgccc tcctcaatcc cgaaacgcaa gacaccgccg cgcgcatcaa cctccatttc
     8341 ggcaacgccg ccgaacagat gcccgccctt gttcaaacac aaggcaaacc cgacatcgtc
     8401 tatctcgacc ccatgtatcc cgaacgccgc aaaagtgccg ccgttaaaaa agaaatgacc
     8461 tacttccatc ggctcgtcgg cgaagcgcaa gatgaagcgg cactcctgca taccgcacgc
     8521 caaacagcaa aaaaacgcgt cgtcgtcaaa cgcccccgcc tcggcgaaca ccttgccgga
     8581 caagcccctg cctaccaata caccggcaaa agcacccgct tcgacgttta cctgccctac
     8641 gggacggaca agggataacg cccataaaac aagacaccga aaaatttgcc gttcttatgc
     8701 aaacgagaaa ccggtttttg cgtttcgact gttttggata aatcatcaca ccttaaagtt
     8761 tgtcattccc acggaagtgg gaatccgatt cattcagttt tatagtggtt taaatttaaa
     8821 ccactatagc tgttttcgag tttcaggcaa cttccaa
//
LOCUS       DAXHSJ010000076         8854 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000076 DAXHSJ010000000
VERSION     DAXHSJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8854
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.076"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..297)
                     /locus_tag="ACFM8U_001240"
     CDS             complement(<1..297)
                     /locus_tag="ACFM8U_001240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6334478.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRH"
     gene            536..1435
                     /gene="crgA"
                     /locus_tag="ACFM8U_001241"
     CDS             536..1435
                     /gene="crgA"
                     /locus_tag="ACFM8U_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC6334479.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSAEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1662..4877)
                     /gene="carB"
                     /locus_tag="ACFM8U_001242"
     CDS             complement(1662..4877)
                     /gene="carB"
                     /locus_tag="ACFM8U_001242"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC6334480.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEELQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQVLGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRS"
     gene            complement(4906..5547)
                     /locus_tag="ACFM8U_001243"
     CDS             complement(4906..5547)
                     /locus_tag="ACFM8U_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFC6334481.1"
                     /translation="MSIPINFNNLKYLLNDMRNKNRIIEAFPFNYNQRQYAVILTRYK
                     PDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFINFFKINVQAGAAKIR
                     EIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIYCYDVRRNGKDKAGKP
                     NRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDAEIIREVTNRQ"
     gene            complement(6226..6615)
                     /locus_tag="ACFM8U_001244"
     CDS             complement(6226..6615)
                     /locus_tag="ACFM8U_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC6334482.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSEAEAKLWQHLRASRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQYAQ"
     gene            complement(7188..7313)
                     /locus_tag="ACFM8U_001245"
                     /pseudo
     CDS             complement(7188..7313)
                     /locus_tag="ACFM8U_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7359..8492)
                     /gene="carA"
                     /locus_tag="ACFM8U_001246"
     CDS             complement(7359..8492)
                     /gene="carA"
                     /locus_tag="ACFM8U_001246"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC6334483.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYASGLIIRDLPLLHSNFRASESLHDYL
                     VRNQTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         1969 a         2270 c         2323 g         2292 t
ORIGIN      
        1 gtgtctgccg tcgctttggt agagtttgcc gtgtccagcg gttaatactt cgtctatgta
       61 ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa tcacagcatc
      121 catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa tcacggtttc
      181 ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa gcgtgcggtt
      241 taacccgccg tgagaatgtc cgaacgcttt gccgccgtct aataataaaa tattcatttg
      301 ttacctcgtt tgtgaattga tgaatgtatt gtgccgtttt actttataat ttaaaagtgc
      361 aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgttccaac acacgggatg
      421 gcacataaag caccgccctg tgtgccgtcc tgattcggaa ggggttacac ccctcccaaa
      481 taaagtctga tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa acacgatgaa
      541 aaccaattca gaagaactga ccgtatttgt tcaagtggtg gaaagcggca gcttcagccg
      601 tgcggcggag cagttggcga tggcaaattc tgccgtaagc cgcatcgtca aacggctgga
      661 ggaaaagttg ggtgtgaacc tgctcaaccg caccacgcgg caactcagtc tgacggaaga
      721 aggcgcacaa tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag cggcggaaac
      781 cgaaatgctg gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt ccgcgatgcc
      841 gatggtgctg catctgctgg cgccgctggc agcaaaattc aacgaacgct atccgcatat
      901 ccgactttcg ctcgtttctt ccgaaggcta tatcaatctg attgaacgca aagtcgatat
      961 tgccttacgg gccggagaat tggacgattc cgggctgcgt gcacgccatc tgtttgacag
     1021 ccgcttccgc gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc cgcaatctgc
     1081 agaagagctt gccggtcacc aatgtttagg cttcaccgaa cccggttctc taaatacatg
     1141 ggcggtttta gatgcgcagg gaaatcccta taagatttca ccgcacttta ccgccagcag
     1201 cggtgaaatc ttacgctcgt tgtgcctttc aggttgcggt attgcttgct tatcagattt
     1261 tttggttgac aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg aacaaacctc
     1321 caataaaacg caccccttta atgctgttta ttacagcgat aaagccgtca acctccgctt
     1381 gcgcgtattt ttggattttt tagtggagga actgggaaac aatctctgtg gataattcta
     1441 taaaaacctg ccataaaatt gtttttactc acttaataac tttgagaaaa aataaataag
     1501 atgatgtata gtcggcggca atggcagtct atatggggag cgtgataggg ctgttcctta
     1561 tgtacggctt gtgtgtggca acatcggctt tccaaatgac aaaatgccgt ctgacaccgt
     1621 aaaaaaaggt ttcagacggc attttcaacc cgattcaggc atcagctgcg gtttttcaaa
     1681 cgcccgtgca gttcttgaac gctgtacacg cccaagtggt cgcggctttt cgcgccttcg
     1741 ctcatcgctt cgccgcccgc agtagtggtg tattgcggca cacgttgctg caatgcgctt
     1801 tggcggatga tgtggctgtc ggacacggat tgcggatcgc tggaaacggt attcacgacc
     1861 agtgcgattt cgccgttttt cagcgcgtcg ccgatgtgcg ggcggccttc ggggacttta
     1921 ttgatggttt gtacaatcag cccgtgttcg gtcaggtatt gcgccgtgcc gcgcgtggcg
     1981 cagatgccgt agcctaaaac ttggaagttt ttagcggttt taatgacgcg ttctttgtct
     2041 tcttcgcgca cggagaggaa gattttgccg gtcgggttga ggcgttcgcc tgcgccgagt
     2101 tgggctttgt agtaggcttc gccgaagctt gcgcccacgc ccatcacttc gccggtggag
     2161 cgcatttccg gtcccaaaat cgtatccacg cccgggaatt tgatgaaggg gaacacggcc
     2221 tctttaacgg catagaaatc ggggacgact tctttttcca cgccttgttc tttcagggaa
     2281 atgcctgcca tgcagcgcgc gccgactttg gcgagcggca cgccggtggc tttggagacg
     2341 aagggcacgg tgcggctggc gcgcgggttc acttccaata cgaacaccac gccgtcttgt
     2401 acggcaaact gcacgttcat cagtccgacc acgcccagcg cgtacgccat cgctttggtt
     2461 tggcggcgga tttcgtcttg gatttcttcg cttaaggagt agggcggcag cgagcagccg
     2521 gagtcgccgg agtggatgcc cgcctgttcg acgtgctgca tgatgccgcc gataaccacg
     2581 tctttgccgt ctgaaacgca gtccacatcg acttcaatcg cgttgttgag gaagaaatcg
     2641 agcaacacgg ggctgtcttc ggaaacctgc acggcttcgc gcatgtattt ttgcaactct
     2701 tcggcagagt gaacaatctg catcgcgcgt ccgccgagga cgtaagacgg gcgcacgacc
     2761 agcggataac cgatttcttc ggctttgacg agggcttctt cttcgttgtg ggcgatgcgg
     2821 ttgggcggtt ggcgcaggcc taagtcgttc aacacttttt ggaagcgttc gcggtcttcg
     2881 gcggcatcga tgctgtcggc ggacgtgccg atgatgttta cgccgttttc aaccaacgcg
     2941 ttagcgagtt tcagcggggt ttgaccgccg taatgcacaa tcacgcccca cgggttttcg
     3001 gtgcggacga tttccaacac gtcttccaat gtcagcggct cgaaatagag gcggtcgctg
     3061 gtgtcgaagt cggtggacac ggtttctggg ttgcagttga ccataatcgt ttcaaaaccg
     3121 gattcgcgca gggcgagcgc ggcgtgaacg cagcagtagt caaactcgat gccctgaccg
     3181 atgcggttcg ggccgccgcc gagaatcatc acttttttac ggtcggaagg acgggattcg
     3241 cattcttctt cgtaagtgga gtaaagatag gcggtttcgg tggcgaactc ggcggcgcag
     3301 gtgtcaacgc gtttgtaaac cggatgcagc ttcagcgcgt agcggtgttc gcgcacttct
     3361 ttttcgctta cgttcaacaa ttgtgccaaa cgtttgtcgg agaagccttt gcgtttcaga
     3421 cggcgtaggg cggcgaaatc caaatcactc aaaatgccgt ctgaaaccgc tttttcttcc
     3481 ttcatcaagt cttcgatttg cgccaggaac caagggtcga tggcgcagat ttcgtggatt
     3541 tcttccagcg tgaagcccgc gcggaacgcg tctgccacaa acagcatacg ttcggggccg
     3601 gggttcgcca gttcgcggcg gatttccgct ttgtcggagc ttctcggatt gaagccgcac
     3661 aagcctgttt ccaagccgcg cagggctttt tggaagcttt cctgaatcgt gcggcccatc
     3721 gccatcactt cgcccacgga tttcatctgc gtggtcaggc ggtcgtctgc ggcagggaat
     3781 ttttcaaatg cgaaacgcgg gattttggtt accacatagt cgatggaagg ctcgaacgac
     3841 gcgggggttt tgccgccggt gatgtcgttg cgcaactcgt ccagcgtaaa gccgaccgcc
     3901 agttttgccg ccaccttcgc aatcgggaag cccgtcgctt tggaagccag cgcggacgaa
     3961 cggctcacgc gcgggttcat ctcaatcaca atcatctcgc cgttttcagg gttcaccgca
     4021 aactgcacgt tcgagccgcc cgtgtccacg ccgatttcgc gcaataccgc cagcgaagcg
     4081 ttgcgcatga tttggtattc tttgtccgtc aacgtttgcg ccggcgcaac cgtaatcgag
     4141 tcgcccgtat gcacgcccat cgggtcgaag ttttcaatcg agcagatgat gatgcagttg
     4201 tcgtttttat cgcgtacgac ttccatctcg tactctttcc agccaaggac ggactgctca
     4261 atcagcagct catgcgtggg cgaggcatcg aaaccgcgtt cgcaaatcgc caaaaactcg
     4321 tctttattgt aggcaatacc gccgcccgaa ccgcccatcg tgaaagaagg acgaatcagc
     4381 gtcgggaagc cgacctgctc ctgcgccgcc aaagcttcgt tcatcgtgtg gcagacaaaa
     4441 gatttcgggc aagacaaacc gattttttcc atcgcttctt taaagcggcc gcggtcttcc
     4501 gccttgtcga tcgcgtcttc cgtcgcgcca atcagctcga cattgtattt tgccagcacg
     4561 ccgttgcgtg ccaaatccag cgcacagttc agcgcggtct gaccgcccat cgtgggcaga
     4621 atcgcatcag gccgctcctt ggcgataatc ttttccaccg tctgccacat aatcggctcg
     4681 atgtaggtaa catccgccat ttcagggtcg gtcatgatcg tggcgggatt ggaattcacc
     4741 agaatgactt tatagccttc ttcacgcaaa gccttgcagg cctgtgcgcc cgaatagtca
     4801 aattcgcagg cctgaccgat aacgataggg ccggcgccga tgataaggat ggattttagg
     4861 tcggtacgtt tgggcatggt gggtctttct tttgaagaat ttggattatt gacgattggt
     4921 aacttctcta attatttctg catctgtttt ttcatcgctt ggattatctg aaaagaaaaa
     4981 actgtaatta ctatcggctt taaatttttc gtatagctct gggcgcaaaa tttttgcttt
     5041 ttcactattt tccacgctcc tgcgattagg cttgccagcc ttatctttcc catttctacg
     5101 gacatcatag caataaatag tgttaggaga attaggttct aaacgagtag ctacaatctt
     5161 tttataaatt atgtctaaat cttttttagt ttgtgttgga atgaaatctg caaaaagatt
     5221 actaaaactc tgaaaaattt ctctgatttt cgcagcacca gcctgaacat taattttaaa
     5281 aaaattaata aaatcagtag cacttttaaa atgaacttca taaaaatcag catacgcaaa
     5341 tattgaattt tcactattca aattaaaaaa ctctaatttt gcttgtgcat aatcatctgg
     5401 tctaggttca tcaggtttat acctagtcaa aataacggcg tattgccttt gattataatt
     5461 aaaaggaaat gcttcaatta ttctattttt gtttctcata tcattaagca aatactttaa
     5521 attattgaaa ttaataggta ttgacatatt taaactcctg cgaatatgtt gcaataaacg
     5581 tagcaatcgt aggttgggtt gaaaacccaa cacaaaccgc ttaattgtct aaaatgttgg
     5641 gtttgctaac ctaacttgta tatcgaaacc cccgtattgt agcaagccgt agcccgtatg
     5701 tagggtatgt gcggtacgta tgcacgcgtt ctccattttc cctgcaaccc caacccaccg
     5761 cgtgcgtgcc ttgcggcaca ctctctgccg atgggtttaa aggtcgtctg aaaaaacgct
     5821 cgcggcgttt gttccatctg ccttgcgata aaggctgcct gaaaaattcc cgctgttgat
     5881 atttcgggca ttctttctca aaccgcctgc aaccgcaaaa tccgctgcaa tctgccccct
     5941 cccccgtggg ggaggaccgg ggagagggca ttcttcgaat ttcggcaacc ttccccaaca
     6001 ccctaaccgc tcaaacacaa gccttgcggc ttgttgccct ctctctggct ctctcccacg
     6061 ggagagagga cgtggtggct gttggaatta agggttctgc aggaaaaaca ggttgtgcgg
     6121 gctgtttttt gtttttcaga cggtcttcta aacgctcaat tcaagcagcc tgtaccctac
     6181 ctgtatccta taattttcaa aactctaatc aaaattaacc gtttgttatt gcgcatactg
     6241 cttttccaat tcctgcaata cgcgcaggat ttccgccagt acatcgtttg tctgctgcaa
     6301 aatttcgtga ttccaaaaac gcagcacggt aaagcccagg ctgttgagat atgccgtccg
     6361 cgcgtggtcg tatacggctt gttccgcgtg ctgcccgccg tctgcttcga caatcagctt
     6421 gggcgttacg cacataaaat caacaatata gtttcccatc ggctgctggc ggcggaattt
     6481 gtagccattt agacggcttg cccgcaggtg ttgccacaat tttgcttctg cctcgctcat
     6541 ttcttggcgc atggctttgg cgcgttggcg caagatgggg ttgtcggtgg tcaagagttt
     6601 ctcgtgcggg ttcattttgt tgtcgtgctg tttgctgccc tctctctaac tctctcccac
     6661 ggggagagag gacggggcgg ttgttggggt taaaggggct gtaaggaaaa caagtcgttc
     6721 aggccacttt ttacttttca gacgacctgc aatcaacggt tgtaaatcca agttgctttt
     6781 caaaccgcct gtaaccgcaa aacttgctgc aacctacccc ctcccccgtg ggggagggct
     6841 ggggagaggg cattcttcga atttcggcaa ccttccccaa caccctagcc gcccaaacac
     6901 aagccttgct gtttgttgcc ctctctctaa cgctttccca cagggagaca ggacggggcg
     6961 gctgtagtga ttaagggttt tgcaaggaaa acaaggaaaa caagttgtac aggctgtttt
     7021 ttatttttca agcgacctga aatcaacgct tgtaaatcca agtcgctttt caaactgcct
     7081 gcaaccgcaa aatctgctgc aacctacccc ctcccccgtg ggggagggct ggggagaggg
     7141 cattttccaa gttgcggcaa tctttcccaa atcccttagt cttctaaaat acaagcctta
     7201 cggttgttgc caaatgaaac ttcttccata cccaaaactt gcgtgtaaaa cgcaatggtt
     7261 cggtcaatgt cggcaacggt cagtaccaga tggtcgagtg cgctaatctt catttcccgc
     7321 tttctttttc aggcggcggc agtgccgtct gaaagccatt atgcccgttt tgccgctttc
     7381 atattgccaa tgaatttgtc aaacaaatag ccgacatctt gcggaccggg gctggcttcg
     7441 gggtggcctt ggaagcagaa cacaggtttg tcggtcagct cgatgccttg caaagtgttg
     7501 tcaaacaagg atttgtgggt aatgcgtgcg ttagcgggca gggtgtcggc atcaacggca
     7561 aaaccgtggt tttggctggt gatgacgact ttgccgctgt ccaaatcttg cacagggtgg
     7621 ttcgcaccgt ggtggctgaa gcgcattttc agggttttcg cgccgatagc gaggctgatg
     7681 agctggtgtc ccaagcagat gccgaaaatc ggtttgccgc tttccatcag tttttgcacg
     7741 gcttcgatgg cgtaggtgca aggctcgggg tcgccggggc cgttggacag gaacacgccg
     7801 tcgggattga gtgccaacac gtcttgtgcg ctcgtttggg cggggacgac ggtcaggcgg
     7861 cagccgcgcg aggcgagcat acgcaggatg ttggttttca cgccgaaatc gtaggcgacg
     7921 acgtggtaag gctgttcgtc aggggtaacg aaacccttgc ccaattccca ttcgccttcc
     7981 gtccattcgt aagtttccgt gcaggaaact tcttttgcca agtctttgcc gaccatactg
     8041 ccgaacgcgg cgatgagttc ttgcgctttt tcgattgtgg catccgcacc ggtcagaatc
     8101 gcgccgcctt gcgcgccttt ttcgcgcaac agcgtggtca ggcggcgggt gtcgatgtcg
     8161 gcgatggcga cggtttggtt gcgtaccaaa tagtcgtgca ggctttcgga ggcgcggaag
     8221 ttgctgtgca agagcggcag gtcgcggata atcaggccgg aggcataaac gctgcggctt
     8281 tcttcatctt cggcgttggt gccggtgttg ccgatgtggg ggtaggtgag ggtaacgatt
     8341 tgtttgcagt aggacgggtc ggtcaggatt tcctgatagc cggtcatcga agtattgaac
     8401 acgacttcgc cggaagtcga accttcgtaa ccgattgatg tgccgtggaa tacgctgccg
     8461 tcagcgagga cgaggagggc gggggtgctc atgatgggaa tcctgttttt aataaaattc
     8521 ttgacaatgc cgtctgaagg gacttcggca gggggcatcc ggtcggaaaa atccggtcaa
     8581 aaaaaaacac gccgcgcaag atagacgcgt aacgtgcttt tcgggcaagt gcctataccc
     8641 tgaaagcagc atattttaag acgaaacata cccgatttca agcgcaaacg cggcaggcgg
     8701 gctgatatgt tcgcgcgaaa acggaaatcc gtggcgtaaa accgcccgct ccgcgcccac
     8761 tatgcccgtt acgcatttcc ccacgtccgt ccggcgaaac tatgggaata cccgaatcgt
     8821 caggaatacc tgaatcgtca ggaatacccg aacc
//
LOCUS       DAXHSJ010000077         8828 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000077 DAXHSJ010000000
VERSION     DAXHSJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.077"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(175..867)
                     /locus_tag="ACFM8U_001247"
     CDS             complement(175..867)
                     /locus_tag="ACFM8U_001247"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6334484.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(975..2168)
                     /locus_tag="ACFM8U_001248"
     CDS             complement(975..2168)
                     /locus_tag="ACFM8U_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6334485.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="ACFM8U_001249"
     CDS             complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="ACFM8U_001249"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFC6334486.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3021..3950)
                     /locus_tag="ACFM8U_001250"
     CDS             complement(3021..3950)
                     /locus_tag="ACFM8U_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFC6334487.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKL
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="ACFM8U_001251"
     CDS             complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="ACFM8U_001251"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFC6334488.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4778..6985
                     /gene="uvrD"
                     /locus_tag="ACFM8U_001252"
     CDS             4778..6985
                     /gene="uvrD"
                     /locus_tag="ACFM8U_001252"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC6334489.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTRRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLIAGNHAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAQ
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGVRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQMPLPEIIVGILKDSGLTEHYRTQKSDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            7218..7730
                     /locus_tag="ACFM8U_001253"
     CDS             7218..7730
                     /locus_tag="ACFM8U_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6334490.1"
                     /translation="MSQVFKDFDLSSVWKTNSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIALIAVQNGGMFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHALFALDYRECGKNGEPKVVFVELAPDFETFIRSLR
                     HEDEFIDEEI"
     gene            complement(7920..8135)
                     /locus_tag="ACFM8U_001254"
     CDS             complement(7920..8135)
                     /locus_tag="ACFM8U_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334491.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQLVQELEQHLIEAYNLMDTTG"
     gene            complement(8259..8618)
                     /locus_tag="ACFM8U_001255"
     CDS             complement(8259..8618)
                     /locus_tag="ACFM8U_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6334492.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
BASE COUNT         2211 a         2442 c         2195 g         1980 t
ORIGIN      
        1 cctctttcag ttacagcagc ttgatccctt tcccttatcc aacgggggaa ggctaggata
       61 gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca accctctccc
      121 tccgtacggg agggggtgga ttctcgcggg cgaagcccac gctacggtta gcctttaccc
      181 cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt cggtatgcgg
      241 cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc ggaggtggca
      301 gagttcgtgg atgcagacat agtcggcgac gtattcgggc gtgccgatca gccgccagtt
      361 gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt tgctcaggtc
      421 tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg gcagcaggta
      481 ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg cggtttcttt
      541 tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt gggtatccag
      601 cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg aaacaggatg
      661 cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca gccagtttgc
      721 taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca tattgacggg
      781 gcgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc acaaaccgtc
      841 ggaaagagta taggtgaagc gtttcatagt tgtgaatagg tttcagaccg gatacatcgt
      901 ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa aatcaaacat
      961 ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt gatggcgctg
     1021 agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag cgggtgtttg
     1081 tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag ggaaatcggt
     1141 aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt gccgatggtg
     1201 gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt attgcgttcg
     1261 atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc aatcaccgaa
     1321 gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta tcccgccgca
     1381 tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg gctgaccacg
     1441 cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcaggat cgcccgcacc
     1501 gagctttggc tcacttccgg caacgccgat ttgcctttga tacctgcgtc cagcagacgg
     1561 tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt aacaatcgcc
     1621 attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag cggcggattc
     1681 gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac ttgggcgaat
     1741 ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat cacgccgctg
     1801 atcacggcac cggtttccgg tgacagtccg gtcagcaggt tcagacccaa gcctgcgccg
     1861 ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc caagaaatag
     1921 cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga aacggcgaca
     1981 atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag aatcacaaag
     2041 ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa gcccgggccg
     2101 atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttctgcg gtttttttga
     2161 tcagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg cgaaacctgc
     2221 ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg ttttcagacg
     2281 gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt tcctgccgga
     2341 ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg ctgtcggctt
     2401 gcacggcaac caggctgccg tccactgctt tgacctgccc gtcccgcacc atctgcaata
     2461 cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg cggggaacca
     2521 gcgtaccgtc gggcatatag cggcggtcgg cgaatacttc agaaatcaca cccaagcctg
     2581 cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc aatttcgggt
     2641 cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc gccgcttgat
     2701 tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca cggcacaagg
     2761 cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg gacagattca
     2821 tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg atgcgcacgc
     2881 cgttttgttg ggcatacgcc aatgccgccc gaatatcggc aatgctgccg gcgtgttggg
     2941 cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg ccgcagcctt
     3001 cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat ttggttcaga
     3061 taggcttggt ttttgcgccg cagggcggtg gcttctttca agccgattat tttgaatttg
     3121 actttgctgc cgaagcgcac ctgtgccagc ctgcccaaat cggcggcggc aacggtagcg
     3181 attttcggat aaccgccggt ggtttgcgca tcggccagca ggataatcgg tttgccgccg
     3241 ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa aggttgcgac
     3301 agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct ttgcagcgtc
     3361 cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata ttccgacgaa
     3421 ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt ggacaattcc
     3481 tgcgcacctt tgccgatggg gagataatcg cctttttgca gcattctgcc ctgatggccg
     3541 ccgaaaccgg ctttcaggtc ggtgcttctc gaacccatca cttccggcac atcaaatccg
     3601 cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccagttt caaggtctgc
     3661 cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa ttccgcctga
     3721 tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat tatcccgccc
     3781 aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt gcccgccgcc
     3841 aaagcaaccg tgtccatcgc accggcatga ccgatgccgt aacgccggtg tccgtagcgt
     3901 ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa cgtgaatcat ggctcaatcc
     3961 tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga ttcaaatcgg
     4021 ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc gaagcgaacg
     4081 gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg gacgttctcg
     4141 gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa gggaaaccgg
     4201 gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg acgatttcgg
     4261 aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat tcgccgccgt
     4321 agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg tgttcccaca
     4381 catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggta aacacggtca
     4441 gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc gcagcggcaa
     4501 acgcccacaa cttttgctgt ttgcccagtt cggaaggcgc attcagtcgg cagaccaaag
     4561 cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg ttatgtttta
     4621 atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat catcaaaccg
     4681 tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa accgtttcta
     4741 ttaaaatagc gcattccact tttcagacgg catccttatg tttcccgacc aatccgcccc
     4801 caacctgctg caaggcttga atcccgaaca actctccgcc gtaacctggc cgccgcaatc
     4861 cgcacttgtg ctggcgggcg cgggcagcgg caaaacgcgc gtgctgacca cgcgcatcgc
     4921 atggctgttg caaagcggac aagccagcgt gcacagcatt atggcggtaa cgtttaccaa
     4981 caaagccgcc aaagaaatgc aaacccgttt gggcgcgatg attcccatca atgtccgcgc
     5041 catgtggctc ggcacgttcc acggtctctg ccaccgcttt ttgcgcctgc accaccgcga
     5101 cgccggtctg ccgtcttcct ttcaaatcct cgacggcggc gaccagcttt ccctgatcaa
     5161 acgcctgctc aaaagcctca acatcgccga agaaatcatc gcgccgcgtt cgctgcaagg
     5221 ctttatcaat gcgcaaaaag aatccggttt gcgcgcttcc gtgttgagcg cgcccgatcc
     5281 gcacacgcgc cgcatgattg agtgctacgc cgaatacgac aaaatctgcc aacgcgaagg
     5341 cgtggttgat ttcgccgaac tgatgctccg cagctacgaa atgctgcaaa gcaacgaaat
     5401 cctgcgccaa cactaccaaa accgcttcaa ccacattctc gttgacgagt tccaagacac
     5461 caacaaactg caatacgctt ggctgaaact gattgccggc aaccacgccg ccgtctttgc
     5521 cgtcggcgac gacgaccaaa gcatctaccg gttccgtggc gcgaacgtcg gcaatatgac
     5581 cgcgctgatg gaagagttcc acatcgacgc gcccgtcaaa ctcgaacaaa actaccgctc
     5641 cgtcggcaat atccttgccg ccgccaacgc cgtgattgaa aacaacgacg aacgcctcgg
     5701 caaaaacctg cgtaccgacg ccgaagcagg cgacaaaatc cgctactact ccgcctttac
     5761 cgaccttgaa gaagcccaat tcatcgtgga cgaaaccaaa gcccttgaac gcgaaggctg
     5821 ggatttggac gaaatcgccg tcctctaccg cagcaacgcc caatcgcgcg tcatcgaaca
     5881 aagcctgttc cgcagcggca tcccctacaa aatctacggc ggggtgcgct tttacgaacg
     5941 ccaagaaatc aaacacgcgc tcgcctacct gcgcctcgcc gtcaatcccg atgacgacaa
     6001 cgccctcttg cgcgtcatca acttcccacc gcgcggcatc ggcgcgcgca ccgtcgaaaa
     6061 tcttcagacg gcctcaaacg aacaaggcat taccctctgg caagccgcct gcaacgccgg
     6121 cgcgaaagcc gccaaagtcg tcgccttcgt ccgcctgatt gaagccctgc gcaaccaagt
     6181 cggacaaatg cccctgcccg aaatcatcgt cggcatcctc aaagacagtg gcctgaccga
     6241 acactaccga acccaaaaaa gcgacaacca agaccgcctc gacaacctag acgaactcgt
     6301 caacgccgcc atcgagttca aacccgaaga cagcaacttt gaaaccctgc ctgaaaacat
     6361 ttcagacgac cccgccttcc ccatcctcgc tttcttgagc aacgccgccc tcgaatccgg
     6421 cgaaaaccaa gcaggcgcag gcgaaaaagc cgtccaactc atgaccgtcc acgccgccaa
     6481 aggcttggaa ttcaacgccg tcttcctcac cggcatggaa gaaggccgct tccccagtga
     6541 aatgagcctt gccgaacgcg gcggcctcga agaagaacgc cgcctgatgt acgtcgccat
     6601 cacccgcgcc cgcaaacgcc tctacattac gatggcgcaa caacgaatgc tgcacggaca
     6661 aacccaattc ggcatcgtct cccgcttcgt cgaagaaatc ccacccgaag tattgcacta
     6721 cctgtccgtc aaaaagaccg cctacgacag ctacggcagc ccgcgccaaa ccgccgcgtc
     6781 caaagacaaa atcatcgacg actacaaaca gccccaaacc tacgcaggct tccgtatcgg
     6841 acaaaacgtc cgccacgcca aattcggcac cggcgtgatt atcgatgccg cagacaaagg
     6901 cgaatccgcc cgactgacca tcaatttcgg caaacagggc gtgaaagagt tggataccaa
     6961 gtttgcgaaa ctggaagaga tgtagaaggg agatggcaga gaccgtctga aaacgggaac
     7021 aacaattttt gcccaaacct ccgccgtcat tcccgcgcag gcgggaatcc ggacctttcg
     7081 gcacggaaac ttatcggata aaaggtttct ttagattcca cgtcctagat tcccgccgga
     7141 acataaatga cggacggtaa aagccgggta tgaataccca ccctctgtta tcactgagat
     7201 caataaggaa gaacattatg tcccaagttt ttaaagattt tgacttgtcc tccgtatgga
     7261 aaactaatag ttgggcagat gaaaactaca aagaagcccc gtttacccct gaaattttgg
     7321 ctgccgtaga aagtgagcta ggctataaat tgccgcaaag ttttattgca ttgatagcag
     7381 tacaaaacgg cgggatgttt gtcaaaaact gctttccgac cacacagaga aattcgtggg
     7441 cgaaagatca tgtgcaaatt tgcgaggtat cgggaatcgg ttttgaaaaa gaaggaagcc
     7501 tgtgcggcga aacagggcaa aaactttggc tggaagaatg ggagtatccg cccatcggcg
     7561 tgtattttgc caacgacccg tcaggcggtc atgccctgtt tgccttggac tatcgggagt
     7621 gcggcaaaaa cggagagccg aaagtggtgt ttgtcgaact cgcccccgat tttgaaacct
     7681 ttatccgcag cttgcggcat gaagatgagt ttattgacga agaaatataa aacggcggtt
     7741 gaaaaactga aatcattaag tgaaaacggg cgaaataacg ggtaaccgtt tgaatccgta
     7801 agggaaacag ttcggcggaa cgtgccatcc aagacctttg caaagaaaaa ctgtccccga
     7861 cagcattgac attattaaca gaacttatca aatttggagc tatcccaaat ataattcggt
     7921 tatcctgttg tatccattag attatatgct tcaattaaat gttgttctag ctcttgcact
     7981 aattgaggat tgcgaattcc tgatacaatc tcaaattctt ctgcatctat gcaaacacct
     8041 gcataaattt caataacaag ggaacgcaat aattgaagct cttctcttgt taaagaaata
     8101 ataatgtcat cacctgtgta attgattata ttcataataa ttttattttt gcttgtcaaa
     8161 gtaagtttta cctaaggttg gtctaaatgc agtcccacca tcttttgaat ttgggtctct
     8221 aattacaatt gctccagact tatcatccca aattgctctt atgtgtttgg attgtaatct
     8281 tcgaattccc aagaaaaaaa tcgtaataag tttgaaagtg tcaaatccca agtttctttt
     8341 gagcaatatt ctaatatttt atcaatttca cttttaataa tcttatgatc aaactgttct
     8401 aatattaatg cattagacca aaaaaaacct tctttattac aatgatggga aatccattta
     8461 ggagaacaaa tgcaaagtga aaaaatagat gagccttgtt ctccttcgat tccgatatcc
     8521 aaatctatcc atctatggaa attatctgga atttcggggg taaatttttc aaaatcaata
     8581 tcatataaat ttatgctttt taaatccaat ttaatcatta gggctgtcct agataaatag
     8641 ggaaattcaa attaagttag aattatccct atgagaaaaa gtcgtctaag ccggtataaa
     8701 caaaataaac tcattgagct atttgtcgca ggtgtaactg caagaacagc aacagagcct
     8761 gacagcattg tttatacgga ttgttatcgt agctatgatg tattagatgt gagcgaattt
     8821 agccattt
//
LOCUS       DAXHSJ010000078         8728 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000078 DAXHSJ010000000
VERSION     DAXHSJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.078"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(305..1201)
                     /gene="argB"
                     /locus_tag="ACFM8U_001256"
     CDS             complement(305..1201)
                     /gene="argB"
                     /locus_tag="ACFM8U_001256"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC6334493.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKAGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1371..2501
                     /locus_tag="ACFM8U_001257"
     CDS             1371..2501
                     /locus_tag="ACFM8U_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC6334494.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNVIGDLR"
     gene            2572..3423
                     /gene="lgt"
                     /locus_tag="ACFM8U_001258"
     CDS             2572..3423
                     /gene="lgt"
                     /locus_tag="ACFM8U_001258"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC6334495.1"
                     /translation="MIIHPQFDPALISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3506..4168)
                     /locus_tag="ACFM8U_001259"
     CDS             complement(3506..4168)
                     /locus_tag="ACFM8U_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC6334496.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4263..6056)
                     /locus_tag="ACFM8U_001260"
     CDS             complement(4263..6056)
                     /locus_tag="ACFM8U_001260"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC6334497.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6303..7412
                     /gene="proB"
                     /locus_tag="ACFM8U_001261"
     CDS             6303..7412
                     /gene="proB"
                     /locus_tag="ACFM8U_001261"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC6334498.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVSVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7425..8687
                     /locus_tag="ACFM8U_001262"
     CDS             7425..8687
                     /locus_tag="ACFM8U_001262"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC6334499.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVTALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KAGLAQTRIDPDALQLIENTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1977 a         2471 c         2279 g         2001 t
ORIGIN      
        1 aaagaaacct tttttcccga taagtttccg tgccgacagg tctagattcc cgcctgcgcg
       61 ggaatgacga aatttcaaag ttatggcgtt atcggaaaaa caaaaaatca aagccggaga
      121 atttatccca aacaaccgga tttcaaaaaa accagattcc cgccttcgcg ggaatgacgg
      181 attttaggtt tctgttttgg tttctgtttt cgcgggaata acaacttaga aattgcccga
      241 aaccccaaaa acagaaaccg gacaaacggg ttttccgcca aagccggcat tttccgactt
      301 tgcttcaggc atcttcccca ccgcccaaaa tcatcgaacc gataccggca tcggtaaaga
      361 tttccagcaa aagcgcgttg ggcaacctgc cgtcgatgat atgcgtggct ttcacaccgt
      421 tgacggcggc ttcgaccgca gaagcgattt tcggcagcat accgccatac agcgtgccgt
      481 cggcaatcag ttcatcaatc cgtttcggcg tgagtttggt cagcagattg cccgttttgt
      541 ccatcacacc ggcgatattc gtcatcatca agagtttttc agcgttcaat tcttccgcca
      601 atttgcctgc caccaaatcg gcgttgatgt tgaacgcttc gcctttttca cctacgccga
      661 cgggggcgac gacgggaatg cagccgcgtt ctatcagccc tttaaccaaa ccggtatcga
      721 tgctttccac cgtaccgacc tgtccgatgt ccacgccatt ctgttcgggc gtatcgatca
      781 aaagtttctt cgccttaatg aaatgatcgt cgcgtccgct cacgccgacc gcgtgtccgc
      841 catatgtgtt aatcatcgac acgatttctt tattgacatg cccgcccaac accatttcga
      901 caatatccat cgcctctttg tcggtaacgc gcattccttg gacaaactca ccctttttgc
      961 cgactttttc aagcatcgca ttgatctgcg gcccgccgcc gtgaacgatg acgggatgaa
     1021 tgccgaccag cttcagcagc acgacatcgc gggcaaaccc tgctttcaag gcaggttcgg
     1081 tcatcgcgtt gccgccgtac ttgatgacgg cgaccgaacc ggaaaaccgg cggatgtaag
     1141 gcagcgcttc ggcaaggata cgcgctttgt cggcggcgga aataatgttt tcagactcca
     1201 taacgactcc ctattgttga caatttcggc ttatgttaac gccaaacgcc gtctgaaaca
     1261 atcttcagac ggcatccggc acaccttcgg gcaggttgaa gccttgcgat acgggcggta
     1321 aaatcgcggt ttatccggtt cgagaaaccg gcgacagcaa ggactttccc atgattaaaa
     1381 tcagcacccg cttcgatgcc ggctcggtcg tcgtcaaaga cctgaccgac ccttccaaca
     1441 tccgcctcgc cctgcgtccc gacaacgcct ccgattttgc acaatggttc tacttccggc
     1501 tgcaaggcgc ggcatatcaa aactgcatca tgcattttga aaacgcggca gaagccgcct
     1561 acccgaaagg ctgggaaggc tatcaggcgt gtgcctcata cgaccgccgc aactggttcc
     1621 gcgtaccgac ttcctacgaa aacggcgtac tgaccgtcaa tcatacgccg ctgtccaaca
     1681 gcgtgtatta cgcctatttc gaaccttact ccgaagaaca gcatttaaac ctcctcggcg
     1741 acgcgcaagg cagcggcctg tgccgcatcg acgacttggg cagcaccgtg caagggcgcg
     1801 acatcaatct gctgaccatc ggcaatcagg tcgaaagcga tctgaaaatc tggattaccg
     1861 cccgccagca tccgggcgaa accatggcgg aatggtttgt cgaggggctg ctcggcaggc
     1921 tgctcgatcc gcaagacccg acagcgcgca ccctgctcga ccgcgccacg ttctatatcg
     1981 tccccaatat gaaccccgac ggttcggcac tgggcaacct gcgtaccaac gccgccggtg
     2041 cgaacctcaa ccgcgaatgg gaaaatccga ctttggaaaa aagccccgaa gtgttcttcg
     2101 tgcgcgaaaa aatgctggaa accggcgtgg atttgttctt ggacatccac ggcgacgaag
     2161 gactgccctt tgtctttgtt gcaggtacgg aaggcgtgcc gaactacaat ccgcgcattt
     2221 ccgcgttgga aacacagttc aaaaccgccc ttttgaacgc cagccccgat ttccaagacg
     2281 aatacggtta cgaaaaagat gcgcccggtc aggcaaacat gaccttggca acaaactggg
     2341 tcggcaaccg tttcaactgc cttgcctata cgttggaaat gccgttcaaa gacaatgcca
     2401 acctgcccga cgacgatttc ggatggaacg gtcaacggtc gttgcgcctg ggcgaagccg
     2461 cgttgtccgc catcctcaac gtcatcggcg atttgcgctg aaacccttat tgcagaaatg
     2521 ccgtctgaag catcatgcct ttcagacggc atttgaaaac gaaagcctgt tatgattatc
     2581 catccccaat tcgatcccgc cctcatcagt atcggcccgc ttgccgtccg ctggtatgcc
     2641 ctaagctaca tcctcggatt tattcttttt acctttctcg gcagaaggcg catcgcgcaa
     2701 ggcttgtccg tttttaccaa agaatcgctc gacgacttcc tgacatgggg cattttgggc
     2761 gtaattttgg gcgggcgttt gggttacgtc ctgttttaca agttttccga ctacctcgcc
     2821 catccgcttg atattttcaa ggtatgggaa ggcggaatgt cgttccacgg cggctttttg
     2881 ggtgtagtta ttgccatatg gttgttcggc cgcaaacacg gcatcggctt cctcaaactg
     2941 atggacacgg tcgcaccgct cgttccgctg ggtctcgctt cgggacgtat cggcaacttc
     3001 atcaacggcg aactttgggg acgcgttacc gacatcaacg cattttgggc aatgggcttc
     3061 ccgcaggcgc gttacgaaga tgccgaagcc gccgcgcaca atccgctttg ggcagaatgg
     3121 ctgcaacaat acggtatgct gccgcgtcat ccctcgcagc tttatcagtt tgcacttgaa
     3181 ggcatctgcc tgttcgccgt cgtttggctg ttctctaaaa aacagcggcc gaccggacaa
     3241 gtcgcctcgc tcttcctcgg cggctacggc atattccgct tcattgccga attcgcacgc
     3301 caacccgacg actatctcgg gctgctgacc ttggggctgt cgatggggca atggttgagc
     3361 gtcccgatga ttgttttggg tatcgtcggc tttgtccggt tcggcatgaa aaaacagcac
     3421 tgaccgcctg tgaaaaatac cgtctgaaac atggaaatcc ggtttcagac ggcattttta
     3481 cgtttggttc atggaaacaa acgcttcaat tcttaccgca acacgccttg tattttctgc
     3541 cgctgccgca cggacaggga tcgttcctgc ctgttttttc gccttccctg cggacggttt
     3601 gcggcttgtt gatgaccgcc tgccagtagc ggtagatatc cgccaatgca taaggcaact
     3661 cggattccag ttccgccagc tcgccttctg tgaattgcag acggatagcg ccgttgtcct
     3721 cttcgtcgta aataccgccc aatgccatga tgggataaaa caactcttca aacgcttcat
     3781 catcgacggc ttcaaaccaa tcggtcggca caatatccaa accgtaaaga taagcattgc
     3841 accatgtgta aaaatcgctg ccgccgtctt cgttttcata cagccacaaa tcgggcagtt
     3901 ttttatccga catcgcggcg gttgtttcca tcgccattgc caaaaccagc cgttcgattt
     3961 cggaacgttc ggcggcggta aattgcgatt cgtcgcccaa cacttcgggc agccagtcga
     4021 gcggtgtcaa tttgtccggc ccgctcaaca gcgccgtcat aaaaccttga acctcgtcgc
     4081 aacgcatcgt gttgccttgt tcgcttttgg catccaacaa ttcgctcaac cgccgtttgg
     4141 atgcttcggt aaattttcgg gaatccatca ttttcctttt caaatgggtt ttgcgcccta
     4201 ttatcgccgc aatgccgtct gaagcctttt tatgcttcag acggcatcgt ctgttttcac
     4261 gctcaaatcg taccgctgcc ctgcgctttc ggtttggcgg cactaaattc cagcttgctc
     4321 aaagcggaaa ggtaggcttt ggcggtggcg accaaaacgt cggtatccgc gccctgaccg
     4381 ttgacgacgc ggttgccgcg cgccagacgg acgctggttt cgccctggct ttccgtacct
     4441 tgcgtgacgg cgttgacgga ataaatctgc aaagccgcgc cgctttgcgc cacgctttca
     4501 atcgctttga aaatcgcatc cacggggccg gagcctgtgg cggaagcgcg tttttcttcg
     4561 cctttgatgc tgaacacgat gtcggcgcgc ggctcttcgc cggtttcagt gctgattttt
     4621 tgggagatga atttgtagct ctcggcattc atgctgccca tttcgtcgga taccagtgcg
     4681 tgcaggtctt catcgaagat ttcgcgtttt ttgtcggcga gttctttgaa gcgggcaaac
     4741 gcggcgttca gtgcctcttc gctttccaac tcgatgccca aatccgccag cttggttttg
     4801 aaggcgttgc ggccggacaa tttgcccaag ctcaaacggt ttgttgccca gccgaccgat
     4861 tcggcggaca taatctcgta agtttcgcgg tgtttcagca ccccatcctg atggatgccc
     4921 gattcatgcg aaaaggcatt ggcaccgaca atggctttgt tgggctgcac gggatagccc
     4981 gtaatggtgg acaccagttt ggacgaaggc acgatttgcg tggtatcgat gccggtttcc
     5041 aagccgaaca agtcgtggcg cactttcaac gccatcacga tttcttcaac cgaagcattg
     5101 cctgcacgtt cgcccaagcc gttgacagta cattccacct gacgcgcacc gccttttaat
     5161 gcggcaagcg aattggcaac cgccaagccc aaatcgttgt ggcagtgtgc cgaccaaacg
     5221 actttgccgc cgttgggcgt tttggcaatc agttcgcgga aaaattcttc ggttttgtac
     5281 gggatggaat agccgacggt atcgggaata ttgatggtgg tcgcgcccgc ttcaatcacc
     5341 gcgccgcaga tttcggcaag gaaatcgatt tccgaacgca acgcgtcttc gcaggaaaat
     5401 tccacatcgt cggtgtattc acgagcgatt ttcaccgctt tgaccgccgc ctcaatcacc
     5461 tgcttcggct tcatcttcaa tttgtactcc atatggatgg ggctggtggc gatgaaggtg
     5521 tggatgcgtt ttttcggcgc gggcgcaacg gcctcacccg cctgacggat gtcccgctcg
     5581 atggcgcggg acaatgaaca gaccgttgat ttggtaatgg ttttcgcaat cgcattgacc
     5641 gcctcgaaat cgcccgggct ggcagcggca aaacccgctt cgatgatgtc cacacccaat
     5701 ttttccagct ggcgggcgac gcggattttt tcctctttgg tcatagcggc gccgggcgac
     5761 tgctcgccgt cgcgcagggt ggtgtcgaaa atgataacgc ggttggcctg tgtcatttct
     5821 gttctctcca gagattcgtt tttttgtaaa aaagcattca aatccagcgg cctgccttgc
     5881 gattcggcaa gcgcggtcag cttttgcagt tgcgccaaag gcagcgaccc gcgcgtgcgc
     5941 catttataga tggcggcggt cgtcgcggca ttttcgggat cgtgctgttt caacgcttcg
     6001 gcaagcgcgt tcacgccgcc gaaataagca accaagcggt ctatatctaa ttgcatggta
     6061 gtccttgtct aaaataaatc agcctacagc agaaatatgg acaggattat acgcattttg
     6121 gacaaaacgg caatataaat ttgaaaaaat ctcaataaac cgccatagat tttattaatt
     6181 ttatacattt tgtccatttt attgcagaca cggcgtgatt gttgacaatt gcggcagaac
     6241 agggcaaatt cggcggcaca aggcggcaaa cgagacagga caaaccaaca aggacggaaa
     6301 caatgaaata caaaagaatc gtatttaaag tcggcacatc ttcgattacc cattcggacg
     6361 gcagtctctc gcgcggcaaa atccaaacca tcacccgcca gcttgccgca ttgcatcatg
     6421 cgggacacga gctggtcttg gtatcttccg gcgcggttgc ggcagggttc ggtgcgctgg
     6481 gtttcaaaaa acgtccggtc aaaatcgccg acaaacaggc ttccgccgcc gtcgggcagg
     6541 ggctgctgat ggaagaatat acggcaaacc tgtcttcaga cggcatcgtg tccgcacaaa
     6601 tcctgctcag ccgcgccgac tttgccgaca aacgccgcta ccaaaatgcc ggcggcgcac
     6661 tttccgtgct gctgcaacgc cgcgccgtcc ccatcatcaa tgaaaacgat acggtttcgg
     6721 ttgaggaatt gaaaatcggc gacaacgaca cattgagtgc gcaagtggcg gcgatgatac
     6781 aggcagacct cttggtgctg ctgaccgaca tagacggtct ttacacgggc aacccgaaca
     6841 gcaatcccga tgccgtacgg ctggacaaaa tcgaacacat caaccatgaa atcatcgaaa
     6901 tggcgggcgg ctcgggttcg gcaaacggca caggcggtat gctgactaaa atcaaagcgg
     6961 cgacgattgc gaccgagtcc ggcgtatcgg tctatatctg ttcctcgctc aaacccgatg
     7021 cacttgccga agcggcagat aatcaggcgg acggctcgtt tttcgtcccc cgtgccaaag
     7081 gtttgcggac gcagaagcaa tggctggcgt tctattccga aagccggggc agcgtttatg
     7141 tggacgaagg tgcggaacac gctttgtccg aacaggggaa aagcctgctg atgtcgggca
     7201 ttgccggaat cgaagggcat ttttcccgta tggacaccgt aaccgtgtac agcaaggcaa
     7261 ccaaacagcc cctgggcaaa gggcgcgtcc tgttcggttc tgccgccgcc gaagacctgc
     7321 tcaaatcgcg taaggcgaaa ggcgtgttca tccatcggga cgattggatt tccatcacgc
     7381 ccgaaatacg cctgcttctg accgaatttt agaaaaggaa acccatgtca aacacacaaa
     7441 aacagcttgc ccttgccaaa gcggcaaaaa aatccgtcaa cacggcggat acggaagaaa
     7501 aaaaccgcgc gctgcttgct atggcggaca gtctggaagc ggcggcggca gatattttgg
     7561 cggcaaaccg tcaagacttg gaagccgcgg caggcaacat tcccgaaagc atgaccgacc
     7621 gccttttgtt ggacggcaaa cgcatttgcg cgatggcgga cggcattagg gcggttaccg
     7681 cactgcccga ccccgtgggc gaaatactgg aaacctcgac tctgcccaac ggcttggaaa
     7741 tcgtcaaaaa gcgtgtggcg atgggcgtta tcggcattat ttacgaaagc cgcccgaacg
     7801 ttacgtccga tgcggcggct ttggcactga aaagcggcag cgcggtcgta ctccgcagcg
     7861 gcaaagatgc attccaatcc gcacgcgcca tcgttgccgc actcaaagcc ggtttggcgc
     7921 aaacccgcat cgaccccgac gcgctccagt tgattgaaaa cacagggcgt gagggcagtt
     7981 acgaaatgat gagggcgaaa gattatctag acctgctgat tccgcgcggc ggggcggggc
     8041 tgatacgggc ggtggttgaa aatgccgtcg tgccggtcat tgaaacggga acgggcattg
     8101 tccacattta tatcgacaag gacgctgatt gggataaggc actccgtatc gtgtacaacg
     8161 ccaaaaccag ccgtccgtcc gtgtgcaact cgatggaagt gctgctggtg catgaagaca
     8221 ttgctgccga cttcctgccc aagctcgaac gactgttggt tcgcgaccgc atagaagccg
     8281 gactgccgcc cgtccgcttc cgtttggatc cgcaggcggc gcggcatatc ggcggcgaag
     8341 cggcgggtgc agacgatttc gataccgagt ttttagacta catcctcgcc gtgaaaaccg
     8401 tcgcatcggt cgaagaggcg gtcgggcaca tcgaagccca cggcacacac cattcagacg
     8461 gcatcgttac cgaaaaccgc cacgctgcgg actatttcac gacccatatc gattctgccg
     8521 ccgtgtatgt caacgcgtcc acgcgcttta ccgacggcgg cgaattcggc ttgggttgcg
     8581 aaatgggcat ctccacgcaa aaactccacg cccggggtcc gatgggtttg aaagagctga
     8641 cgagttacaa atacatcgta caaggcacgg gacaggttag ggaataatcc gaattcaaac
     8701 gtctgatata gtggattaac tttaaacc
//
LOCUS       DAXHSJ010000079         8706 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000079 DAXHSJ010000000
VERSION     DAXHSJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.079"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(134..1576)
                     /gene="aldA"
                     /locus_tag="ACFM8U_001263"
     CDS             complement(134..1576)
                     /gene="aldA"
                     /locus_tag="ACFM8U_001263"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFC6334500.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEEAIAREPKGGRADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRKRSDELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1684..2601)
                     /locus_tag="ACFM8U_001264"
     CDS             complement(1684..2601)
                     /locus_tag="ACFM8U_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6334501.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            2774..3574
                     /locus_tag="ACFM8U_001265"
     CDS             2774..3574
                     /locus_tag="ACFM8U_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6334502.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLHQDLLG"
     gene            3622..4398
                     /gene="mlaE"
                     /locus_tag="ACFM8U_001266"
     CDS             3622..4398
                     /gene="mlaE"
                     /locus_tag="ACFM8U_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC6334503.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            4449..4943
                     /gene="mlaD"
                     /locus_tag="ACFM8U_001267"
     CDS             4449..4943
                     /gene="mlaD"
                     /locus_tag="ACFM8U_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC6334504.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVGFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            4980..5570
                     /locus_tag="ACFM8U_001268"
     CDS             4980..5570
                     /locus_tag="ACFM8U_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC6334505.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            5627..5905
                     /locus_tag="ACFM8U_001269"
     CDS             5627..5905
                     /locus_tag="ACFM8U_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC6334506.1"
                     /translation="MHTELNNGTLHISGDITVKTLTGEAFERFQQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            5902..6729
                     /locus_tag="ACFM8U_001270"
     CDS             5902..6729
                     /locus_tag="ACFM8U_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC6334507.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            6854..7216
                     /locus_tag="ACFM8U_001271"
     CDS             6854..7216
                     /locus_tag="ACFM8U_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC6334508.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7216..7599
                     /locus_tag="ACFM8U_001272"
     CDS             7216..7599
                     /locus_tag="ACFM8U_001272"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6334509.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            7596..8084
                     /locus_tag="ACFM8U_001273"
     CDS             7596..8084
                     /locus_tag="ACFM8U_001273"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC6334510.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLSDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8166..8294)
                     /locus_tag="ACFM8U_001274"
     CDS             complement(8166..8294)
                     /locus_tag="ACFM8U_001274"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC6334511.1"
                     /translation="MAGVTVGRGSVVGAGAVVTKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(8300..8680)
                     /locus_tag="ACFM8U_001275"
     CDS             complement(8300..8680)
                     /locus_tag="ACFM8U_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC6334512.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
BASE COUNT         1945 a         2508 c         2282 g         1971 t
ORIGIN      
        1 taaatttaat ccgctataca aagacaaaaa catcgacctc ggtcatgact gattgccgat
       61 gaagcaataa aaatgccgtc tgaacctgaa aacgggttca gacggcattt tctatcgggg
      121 ttttaaagcg gcattaaatg tcggtttcca aataaacgac ttgggtttgc agatattctt
      181 ccaaaccgtg tttgccgtcc gcgccgccga taccggattt tttccaaccg gcgtggaaac
      241 cctgcatggc ttcgaagttt tcgcggttga tgtaggtttc gccgaattgc aggcggcggg
      301 taacgtagaa ggcttcgttt aaattagtcg tataaacaga actggtcaga ccaaactcgc
      361 aatcgttagc cagtgcgatg acttggtcga gcgtgtcgaa agcggaaacg ggcagcacgg
      421 ggccgaaggt ttcttctttc ataatgtcca tactgttgtc ggtgtcggtc agcagggtcg
      481 gctcgaagaa atagccgcgc ccttcggcgc gtttgccgcc gcagaccagt ttcgcacctt
      541 gtttgactgc ccgttccact ttttcggcaa cggctttgac ggcgcgctct tcaatcagcg
      601 gacccatttc cagcgcgcct gcttcggctt cggcagggtt gccgtagcgc acgcctttca
      661 tggcggcggt cattttttca atgaacgcgt ctttcaggct gctgtggaca tagacgcgct
      721 cggcgcagtt gcagatttga ccggtgttgc cgacgcgcga agccaagatg gatttcaccg
      781 ccaaatccaa atccgcgtct ttcaaaacga tggcgggggc tttgccgccg agttccagcg
      841 aaactttggt gatgttggcg gaagcggctt ccatcacttg gcggcctgct tcgacggagc
      901 cggtcaggct gaccatatcg acttgcggat gggcagacaa ggcattgccg atttccgcac
      961 ccggaccgtt caccacgttg aacacgccgg cgggcaatcc gaccgcatcg acgatttcgg
     1021 cgaagatgtg gcagttgatc ggggttacgc tgctgggttt gacaacgatg gtgttgcccg
     1081 ttactaaggc gggacccatt ttgcgggcaa tcaggaagaa agggaagttc cacggcaaaa
     1141 tgccggcaat tacgcccagc ggacgtttga acaataaaat attttcacgc gggcggtcgc
     1201 tttggatgat ttcgccttcg taacggcgcg cccattcggc ttggtaatcg agatagtcgg
     1261 cggtgaacat cacttccacg cgcgccaagt ctttggtttt gccgccttcg gcaacaatgg
     1321 tgtcggtcag ctcgtcggag cgtttgcgta tgccttgggc gattttgcgc aaatacgcgc
     1381 cgcgttcgac cgcaggcaga cgttcccaag ccggttgcgc cgcacgcgcc gccgctacgg
     1441 cgcggtcaac gtccgccctg ccgcctttgg gttcgcgggc gatggcctct tcggtggacg
     1501 ggttcaatac gtcgcgccat tcgccgttga aatcgttttc aaagcgtccg ttgatgtaca
     1561 ttgccaattg tttcatttcg ggttctccgg ttttgtagtc agatgtagtt ttagtttatt
     1621 cccaaataaa ttgccgtaca agtttcttta cacgcggatt tcgggtttga aatcaaatgc
     1681 ggcttaccgg ttttcttttt tggtttccgc aggcgtgctg ccgaaatatt gcctgaatgc
     1741 ctgagtaaag ctggaaacgt ggcggtagcc gcacaaatac gcggtttcgc ctatgctttt
     1801 gcccccgttt tgcaacaggt agagcgcgtg ctgcatttgt ttgtggtgca gccactcgct
     1861 tgccgtaatg ccgaaatggt cgcgcatacg gcgttgcagc gtcctttcac tgatgttcag
     1921 cgcgtctgtc agccggttga cttggtgtgc gccgtcggca aacgcggcat ttagggtgcg
     1981 gctgaagtct tcagacggca tagcgtctgc ttccgccgtt tgccccgccg ccggcccgat
     2041 gccgtctgaa accgtgtccc acaagtccga cagcagccgc aacacgtccg cctcgcggcg
     2101 caatgtttcg cccaaatgcc cctttgggac ggctttcagg caggatgccg ccaagccgcg
     2161 caggttcggg ggcaaatccc atatcctgac cggttcgcgg taaagcaggg gtgcgaaacg
     2221 cgcgtattcc ggacgcagca gccattgttc cataccttta atggtcattt tgaccgtttt
     2281 gccgcctcgg taaagatagc ggctgaacag gacttcttcc ccgacagcaa ttaggacaat
     2341 cttgccgccg tccgcatcga tttggaagcg gcagcggttg atgccgaagt ccaaactgcc
     2401 ttccagcaag agcacgaacg acacataagg ttcggcgagg cggctgctgc aaaaatcaca
     2461 ccgtgccgtt actgtgccgc cgtggatgga aatgccgttg gacaaagtat cgaaacggta
     2521 gctgccctcc acatagctgc cgccgccgct tttgactaga ttgacgaacc ggctgctgtt
     2581 caaatgttct gctttttcca tagttttctg ataaaggtta tcatttgaaa gataacattt
     2641 ttcgccacag caaacaatcc gccgccgaca acggcatttt cacaacattt accgtccgta
     2701 tgccgtctga aaattgcccg tcttcccgat aagccttaaa atacacgccg tcaaacctac
     2761 cgtccgtccg tacatgagcc catccccctt tatcgaaatg aaagacgtcg ccttcgcgta
     2821 tggcgaccgc ccgattctga agaacatcaa tttcagcatt ccgcaaggca attttgccgc
     2881 cgtaatgggc ggttcgggca gcggcaaaac cacgctgatg cgactgatta caggacagat
     2941 tcgtccgcag tccgggcagg ttttgattga aggacgggat ttggcgggct tttcggctga
     3001 cgagttgtat gaacaccgcc gccgtatggg cgtattgttc caacacggcg cgctgtttac
     3061 tgatttgtcg gtattcgaca atatcgcttt tccgatgcgc gaactgacgc gcctgccgga
     3121 agcggtgatt cgggatttgg ttttgttgaa attgaacgcg gtcggtctgc gcggtgtgga
     3181 aaacctgatg ccgtccgagt tgtccggcgg gatgtcgcgc cgcgtcgcgc ttgcccgcac
     3241 gattgcgctc gaccctgaaa ttatgttgta cgacgagccg tttaccggcc tcgatccgat
     3301 ttccttgggc gtgattgccc acttgatcag ccgcgtcaac aaggctttgc gttcgaccag
     3361 tattatggta acgcacgaca ttgaaaaatc tttggaaatc gtcgatcagg tgattttctt
     3421 ggcgcacggc gaaattatgt tctccggctc gccgcaggaa atgcgcgaac tggattcgcc
     3481 ttgggtgcgc cagtttgtcg gcgggctggc agacggtccc gtagcatacc gctatccggc
     3541 gcaaacgtcg ttgcatcagg atttgctcgg gtaaatgagc cggtgccgtc tgaaaccgtt
     3601 gaacttcaca tatagaaatt tatgaatttt atccgttccg tcggggcgaa aaccctcggc
     3661 cttattcaat ccctcggcag tattacgctg tttctgctga acattctggc gaagtccggt
     3721 acggctttcg tccgtccgcg cctgagcgtg cgccaagtgt atttcgccgg cgtgctgtcg
     3781 gtgctgattg ttgccgtttc ggggctgttc gtcggtatgg ttttgggttt gcagggctat
     3841 acgcagttgt cgaaattcaa atccgccgat attttgggct atatggttgc ggcttcgctg
     3901 ttgcgcgagc tgggtccggt attggcggct attctgtttg ccagcagcgc gggtggtgcg
     3961 atgaccagcg aaatcggtct gatgaaaacg accgaacagc tcgaagcgat gaacgtgatg
     4021 gcggtaaacc ctgtcgcccg cgtggttgcg ccgcgctttt gggcgggcgt gttttccatg
     4081 ccgcttttgg cttcgatttt caacgtggcg ggtattttcg gcgcgtattt ggtcggtgta
     4141 acctggctgg gcttggacag cggcattttc tggtcgcaaa tgcagaacaa catcacgata
     4201 cattacgatg taatcaacgg tctgatcaaa tccgccgcgt tcggcgtggc ggtaacgctg
     4261 attgccgtgc atcagggctt ccactgcgtc ccgacctcgg aaggcatttt gcgcgccagc
     4321 acgcgcacgg tggtttcgtc cgccctgacg attttggcgg tcgattttat attgaccgcg
     4381 tggatgttta cagattgaca gggcgtctga agacgaaacg cggaacatcg gatattcaag
     4441 gaactttaat gaaaaagaac atattggaat tttgggtcgg actgttcgtc ctgattggcg
     4501 cggcggcggt cggctttctc gctttccgcg tggccggcgg tgcggcgttc ggcggttcgg
     4561 acaaaactta cgccgtttat gccgatttcg gcgacatcgg cggtttgaag gtcaatgccc
     4621 ccgtcaaatc cgcaggcgta ttggtcgggc gcgtcggcgc tatcggactt gacccgaaat
     4681 cctatcaggc gagggtgcgc ctcgatttgg acggcaagta tcagttcagc agcgacgttt
     4741 ccgcgcaaat cctgacttcg ggacttttgg gcgagcagta catcgggctg cagcagggcg
     4801 gcgacacgga aaaccttgct gccggcgaca ccatctccgt aaccagttct gcaatggttc
     4861 tggaaaacct tatcggcaaa ttcatgacga gttttgccga aaaaaatgcc gacggcggca
     4921 atgcggaaaa agccgccgaa taaacgtatg ccatcatatt caaactagga cacttaatca
     4981 tgaaaaaatc ctccctcatc agcgcattgg gcatcggtat tttgagcatc ggcatggcat
     5041 ttgccgcccc tgccgacgcg gtaaaccaaa tccgtcaaaa cgccactcaa gtattgagca
     5101 tcttaaaaag cggtgatgcc aacaccgccc gccaaaaagc cgaagcctat gcgattccct
     5161 atttcgattt ccaacgtatg accgcattgg cggtcggcaa cccttggcgc accgcgtccg
     5221 acgcgcaaaa acaagcgttg gccaaagaat ttcaaaccct gctgatccgc acctattccg
     5281 gcacgatgct gaaattaaaa aacgccaacg tcaacgtcaa agacaatccc atcgtcaata
     5341 aaggcggcaa agaaatcatc gtccgcgccg aagtcggcgt acctgggcaa aaacccgtca
     5401 acatggactt caccacctac caaagcggcg gtaaataccg tacctacaac gtcgccatcg
     5461 aaggcgcgag cctggttacc gtgtaccgca accaattcgg cgaaattatc aaagcgaaag
     5521 gcgtggacgg actgattgcc gagttgaagg ctaaaaacgg cagcaagtaa tccataagcc
     5581 atgccgccag actgaaggcg acatggattt tttcctaccg aacactatgc atacagaact
     5641 caacaacgga acactgcaca tcagcggcga catcaccgtc aaaaccctga ccggggaggc
     5701 ttttgagcgt ttccagcagc aatgccgtct gaaagaaacc attgccgtcg atttcggcgg
     5761 tgtcaaacgt gccgattccg cctgcgtgtc gctgctgctc gaagtgctgc gcggctgcaa
     5821 aggcagcgtc aggctgaccg gcattcccga atccgtgcgc gcgctgtccg aactgtacga
     5881 aatcaaagac tggctgaaat catgaaaaaa accgcctatg ccttcctcct gctgatcggg
     5941 ttcgcttccg cccctgcatt tgccgaaacc cgccccgccg acccttatga aggctacaac
     6001 cgcgccgttt tcaaattcaa cgaccaagcc gaccgctaca ttttcgcccc tgccgcgcgc
     6061 ggctaccgca aagttgcgcc gaaacccgtc cgcgccggcg tgtccaattt ttttaacaac
     6121 ctgtgcgacg tggtcagctt cggcagcaat atcttgcgct tggacatcaa acgcgcaagc
     6181 gaagaccttg tccgcgtcgg catcaacacc actttcggtt tgggcgggct tatcgacatc
     6241 gccggcgcgg gcggcattcc cgacaataaa aacaccttgg gcgacacgtt tgcttcgtgg
     6301 ggatggaaaa acagcaatta tttcgtgttg cccgtcttag ggccgtccac cgtccgcgac
     6361 gcgctcggca cgggtattac ctccgtttat tcgcccaaga atatcgtctt ccgcacccct
     6421 gtcggacgct ggggcacgac tgccgtatcc gccgtcagta cgcgcgaagg tctgctcgat
     6481 ttgaccgaca gtctggacga agccgccatc gacaaataca gctacacgcg cgacctctat
     6541 atgaaagtcc gtgcgcggca gaccggtgca acacctgccg aaggtacgga agataacatc
     6601 gacatcgacg aattggtcga aagtgccgaa accggcgcgg cggaaactgc cgttcaagaa
     6661 gattccgtat ccgaaacaca ggcagaagca gcaggggaag ccgaaacgca acctggaaca
     6721 caaccttaaa gccatgccgt ctgaaaatcc gtgttcggac ggcattccct tctgtcggaa
     6781 cggacagtcc gaaaccacag attgggctat aatctctttt ttgcgcatac cgcgcgacag
     6841 caaaggaaaa accatgtacg aagtcaaccg cagcgtattc gtcctcatcc ctttagaacc
     6901 tttttggaac tggctgcaaa ccctgccggg caaccacctc gacggactga ctcttgaaga
     6961 catccaagcc gacgccaatt cctaccttgt ccgcccgtgc gaaaccgccg acgaagtatg
     7021 ggacgaaatc gaagcccgtt ttgaagacat tttcgccgcc gaacttgccg actggtgcga
     7081 agacgaacgc gagtggcctg cgctcgatgc cgacattttc aacgaatggt tcgacatcca
     7141 gctttctacc gtcatcaccg acctcgaaca cgaaccgctt gcccgcgaag ccttccaacc
     7201 catcaacctg aactgatgaa gctgaccgtc cgcaactacc atctcgacgg ctacggccat
     7261 gtcaacaatg cgcgctacct cgaatttttc gaagaagcgc gctgggcgtt ttttgaagaa
     7321 cgcggactgc tgcacgagtt ggcaggcctc atactgattg tcgcccgcat cgacatccga
     7381 tacagccgcc ccgccgtcga aggcgacgta ttgcagtttt catgccgcct gaaaaccccc
     7441 ggcacgcgcc gtattgtgct gacccagacg attacactgc caaacggcaa aaccgccgca
     7501 gaagccgaca tcaccctgat gcccgtccac gccgccacac aacgcaccgt cagcctgccc
     7561 gccacccttg cccgcgcatt ggaagccttg tccgaatgaa aaaaatactc accgccgccg
     7621 tcgtcgcact gatcggcatc ctccttgcca tcgtcctcat ccccgacagc aaaaccgcgc
     7681 ccgctttctc cctgtccgac ctgcacggaa aaaccgtttc caacgccgac ctgcaaggca
     7741 aagttaccct gattaatttt tggtttccct cctgtccggg ttgtgtgagc gaaatgccca
     7801 aaatcattaa aacggcaaat gactataaaa acaaaaactt ccaagtcctt gccgtcgccc
     7861 agcccatcga tccgatagaa agcgtccgcc aatatgtcaa agactacggt ttgccgttta
     7921 ccgtcatgta tgatgcggac aaagctgtcg gacaggcgtt cggcacacag gtttatccga
     7981 cttccgtcct tatcggcaaa aaaggcgaaa tcctcaaaac ttatgtcggc gaacccgatt
     8041 tcggcaaact ctaccaagaa atcgataccg cgctggcaca atagcgatgc cgtctgaagc
     8101 gcaaaaaaac agaaacgatg ttcctgtttt ttcagatcat ccgccccgac acgttcagac
     8161 ggcattcaac cttccggcag attctttttc accactgccg gattgcctgc cgccaaagag
     8221 tagggcggaa tgtcttttgt aaccaccgcg cctgcgccca cgaccgaacc gcgtccgacg
     8281 gttacgcccg ccattacaat caccctgtgc cccggccaga catcgtcctc caacgtaatc
     8341 ggacggattt ccgtgtagcc ctcaaagcgt tttttgaacg gtcaaacttg tggttatttg
     8401 aataaaacag acattccggc tccatcatca catttttgcc gaccaccagc ccacggcaga
     8461 tttcacagtt tgccccgatg cccgagccgt cgcccaaaac cgtatccgga aacacatacg
     8521 ccccgcgttc gatattgacc ccgcgtccga tatgcggaga aacccgccgc gccaaaaaac
     8581 cgcgcacccg tctgccgacg atgccgatgc cgcgcatatg tgacggcggc aaccccgaac
     8641 cggcaaaacg cagcagcagg aaaaagaatt ttgatcccat caaacctccc tcaaaagaac
     8701 ttttta
//
LOCUS       DAXHSJ010000080         8704 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000080 DAXHSJ010000000
VERSION     DAXHSJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.080"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            197..1732
                     /locus_tag="ACFM8U_001276"
     CDS             197..1732
                     /locus_tag="ACFM8U_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6334513.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMSGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     DEK"
     gene            1729..1821
                     /locus_tag="ACFM8U_001277"
     CDS             1729..1821
                     /locus_tag="ACFM8U_001277"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC6334514.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1881..3101)
                     /gene="lysA"
                     /locus_tag="ACFM8U_001278"
     CDS             complement(1881..3101)
                     /gene="lysA"
                     /locus_tag="ACFM8U_001278"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC6334515.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTASVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQNEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVEPKNIAPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(3112..3282)
                     /locus_tag="ACFM8U_001279"
     CDS             complement(3112..3282)
                     /locus_tag="ACFM8U_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6334516.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSKPQSAPQTQK"
     gene            3353..3676
                     /gene="cyaY"
                     /locus_tag="ACFM8U_001280"
     CDS             3353..3676
                     /gene="cyaY"
                     /locus_tag="ACFM8U_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC6334517.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     KIAEL"
     gene            3703..4719
                     /locus_tag="ACFM8U_001281"
     CDS             3703..4719
                     /locus_tag="ACFM8U_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFC6334518.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4744..5163
                     /locus_tag="ACFM8U_001282"
     CDS             4744..5163
                     /locus_tag="ACFM8U_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC6334519.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPEVLQSFFEYVPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNYVLILWDDACTIAALCINDYIHAVYDFVAQ
                     AGYCRNGFPEAQGEWLKVADRVLDDELLDKILSRKPT"
     gene            5185..5691
                     /gene="luxS"
                     /locus_tag="ACFM8U_001283"
     CDS             5185..5691
                     /gene="luxS"
                     /locus_tag="ACFM8U_001283"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC6334520.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVANVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            5837..8617
                     /gene="polA"
                     /locus_tag="ACFM8U_001284"
     CDS             5837..8617
                     /gene="polA"
                     /locus_tag="ACFM8U_001284"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC6334521.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPSEKQAEKATVPERLDYQA
                     VTTEAQFAALLDKLAQADTIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQYVLGRLKPHLGNPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMRRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSHWLDSAGLKTKLIMQVHDELVLEVPEAELDLVKEKLPQIMAKVDEGMLK
                     VPLVAEVGVGEN"
BASE COUNT         2037 a         2248 c         2293 g         2126 t
ORIGIN      
        1 cccctaaaat ttttcgtttt caagccttca ccgcttgcca tcagcgttaa atttttttac
       61 gataagcaca tagattgtaa acaatcggcc acaagccggt ttgttttttc agaagacatt
      121 atccctgtca gacgctattt ctatatattt cgcctataac ggcttgtttt taataaataa
      181 ctcaagaggt atcaacgtgt ctgattccaa aacgaaagaa cgcgccacat tcggcacgcg
      241 ccgcgcgttt atgattgccg ccatcggatc cgccgtcggc ttgggcaata tttggcgttt
      301 cccctatatt gcttttgaaa acggcggcgg cgcgttcatc ctgccctatc tggtcgcgct
      361 tctgacggcg ggcatcccgc tgctgctgct cgattatgcc atcggccacc gttaccgtgg
      421 ttctgcgccc ttggctttcc gccgcctcgg acgatggttt gagccggtcg gctggtggaa
      481 cgtgatgacc aatatcgtca tctgcatcta ttacgcagtg attatcggtt gggcggcaag
      541 ctatacctat tattcggtca acgctgcctg gggtgcggat ccgcagggtt ttttctttaa
      601 ggacttcctg caaatgtcgg gcccggaagc cttgggtttg gattttgtcg gcaaagtggc
      661 cggtcctttg gcgggcgtgt gggtttttac cgccgccatt atggctttgg gcgtgcaaaa
      721 gggcgtggcg cgcgcctcgt cgttctttat gccgctgctt ttggtgatgt ttttgattat
      781 ggtcggcatt tcgctgacgt tgccgggcgc ggcaaagggc ttggacgcat tgtttacgcc
      841 cgactggtcg aaactcgccg attccaaggt ctgggtggcg gcatacgggc agattttctt
      901 ttcgctttcc atctgcttcg gcattatggt tacctattct tcttacttga agaagaaaac
      961 ggatttgggc ggcacgggct tggtggtcgg ttttgccaac agcagttttg agttgctcgc
     1021 gggcatcggt gtgtttgccg cattgggctt tatggcgcag gcgggcggta aggcggtcaa
     1081 cgaggttgcc tcaggcggca tcggtttggc gtttatcgcc tttccgacca ttatcaacca
     1141 ggcgccgatg ggctggctga ttggcatatt gtttttcggt tcgctggtat tcgccggcgt
     1201 tacgtcgatg atttccatcc ttgaagtgat tgtggcggcg attcaggaca agctgaacat
     1261 cgggcgcgtc aacgccacgc tgctggtctg cattccgatg ggcattgttt ccacgctgct
     1321 gttcggtacg gcgacggggc tgccggtttt ggacgtgatg gacaaattcg tcaacaccta
     1381 cggcattgtt gccgccggct ttgtttatgt tgccgccatc atcatcagcg gcaggctgcc
     1441 ggaattacgc aagcacctga acgctttgtc ctccatccgc atcggcggct tgtggacggt
     1501 ctgcgtcgtg gttaccgtcg tgatgctcgg ctatatgctg tttaaagata ccagcggcct
     1561 gatggagaaa aattacgaag gttatccgga tggtttcctc agtattttcg gctgggggat
     1621 gtcggcggcg ttggtcgtgt tcgggctgct gctgtcgttg ctgccttgga aacacggtca
     1681 ggatttcaac gtcaaagacg aacacgaaca tgaacaagga gacgaaaaat gagtacttcc
     1741 gccattgtga tgatgattgt ctcaatcgtg ataatctggg gagggctgct gctttccctg
     1801 ttaaggctgc cgaacgagta agcctttaga gcgttaaaaa tgccgtctga accgcttcag
     1861 acggcatttt gatgttcggc ttacaggcag gcgagttcgt ttgccatttg ctgttccaag
     1921 gtttcgcgcc ggcggatgag tcggtattcg ttgccgtcca ccaacacctc tgccgcacgg
     1981 ttgcgcgcgt tgtaattgct cgccatactg gccccgtatg cgcccgcgct gcggataagc
     2041 agcaaatcgc cttcttcgca ggcgatggtg cggtctttgc cgaggaagtc gccggtttca
     2101 caaatcgggc cgacgatgtt ggcagtcagc ggcgcgatgt tttttggttc taccgcttcg
     2161 atgtgatgat aggcatcata tagggcgggg cgcattaaat cgttcatcgc cgcatcgacc
     2221 atcacaaagt ttttctcttc gccgtatttg acgaattcga cacgcgtcaa caacgcgcct
     2281 gcgttgccga ccaagctgcg gccgggctca agaatgagtt tcagacggcg tgtgccgatc
     2341 agtttttgaa ccgcttgggc atacgcgccc aaatcaggca ctttttcgtt ttggtaaaca
     2401 atgccgacgc cgccgcctaa gtccaaatgt tccaaaacaa tgccttcggc ggcaagcgcg
     2461 tcaaccaaaa tcaaaatgcg ttcgcaggct tcgaccagcg ggcttaagtc ggttagttgg
     2521 gagccgatgt ggcagtcgat gccgatgatt ttcaaattgg gctgttgtgc ggcatggcgg
     2581 taggcttcga gcgcgtcggc gtaggcgata ccgaatttgt tggctttcaa acctgtggag
     2641 atgtaaggat gggttttcgc atcgacatcg ggattgacgc gcagggagac ggacgcggtt
     2701 ttgcctaaac gcgcggcaac tttttgaata cggtcgattt cgggaatgct ttccatattg
     2761 aagcatttca cgccggcatt cagcgcgaac tcgatttctg cctcgctttt gcccacgcct
     2821 gaaaatatgg tttttgccgc gtcgccgcct gccgccaaaa cgcgtgccaa ttcgccgccg
     2881 gacacaatgt caaaaccgct gcccagcgag gcgaagtgtt tgataatgct cagattgccg
     2941 tttgccttga cggcgtaaca gacgagcggg ttcaaagcgg caaacgcggt ttggtagtgt
     3001 tcaaatgctt cggtcagcgc ggattggctg tacacataaa gcggtgtgcc gaatgcttca
     3061 gcaaggcggg ggtaggggac ttgttcgcaa aatagggtca tgttttcgtt ttcatttttg
     3121 ggtttgtgga gcggattgcg gtttgctttg aagttgcaaa ccggtttgga ttacgccgaa
     3181 acgcgccttg tcgccttctt tgggcaggta gaggtcgcct ttgtaaccgc aggccgagag
     3241 caggagggcg gttgccgccg caaaaaatac gccgtatttc atcggtaaac ttccttcata
     3301 agcgcgaatg tggcaagatt cggcatctta aacaaaaaac acgcaaaaag ctatgatgac
     3361 cgaaagcgag tttatccgcg cgagcgaagc attatttgaa cacatcgaag accaaatcga
     3421 cgaaaacggc tgggatttcg actgccggtt tgccggaaac gtactgacca tcgaagccga
     3481 aaacggcacg caaatcatcg tcaaccgcca cacgcccaac caagaattgt ggattgccgc
     3541 caaaagcggc ggctaccatt tcgccgagca aaacggcaaa tggctggcaa cgcgcgacag
     3601 ccgcgatttt tatgacgttt taaacgaagc cctgagcgcg gcttcgggcg aagcggtaaa
     3661 gattgccgaa ttgtaatttg agtgttatca cggaaagaaa aaatgaacac acgtcccttt
     3721 tatttcggac ttatatttat cgcgattatc gctatacttg ctaactattt aggaaacact
     3781 gatttttccc atcattatca tatcagtgct ttaattattg ctatcttgct gggaatggca
     3841 atcggcaata ccatttatcc gcaattttca acacaagtgg aaaaaggcgt tttgtttgcc
     3901 aaaggcgcgc ttcttcgcac tggcattgtg ctgtatggtt tccgcctcac ttttggcgat
     3961 attgccgatg taggattaaa tgctgtcgtt accgatgcaa tcatgctaat ttcaaccttc
     4021 ttttttaccg cacttttagg cattcgttat ctaaaaatgg ataaacaatt ggtttatctc
     4081 actggggcag gttgcagcat ttgcggtgcg gcagcggtga tggcggcaga gcctgttacc
     4141 aaagcagaat cccataaagt ttcagtggcg attgccgtag tggtcatttt cgggacgctt
     4201 gctattttta cttacccctt gttctacacg tggtcacaac atttaattaa cgcccatcaa
     4261 ttcggtattt atgttggttc tagtgtacac gaagtggctc aagtttatgc gattggggaa
     4321 aatattgatc ctatcgtggc gaatactgcc gtcatttcca aaatgatccg agtgatgatg
     4381 ctcgccccct ttttattaat gctttcttgg ttattaacac gtagtaatgg agtatcagaa
     4441 aatacatcac acaaaattac aattccttgg tttgctgtac tttttattgg tgttgccatt
     4501 tttaattctt ttgatttatt accaaaagaa ctcgtgaaat tattcgttga aatcgattct
     4561 ttcttattaa tttcatcaat ggctgcgctt ggcttaacga cgcaagcaag cgcaatcaaa
     4621 aaggcaggat taaaaccgtt tgttttagga atactaactt atttatggct agtggttggt
     4681 ggatttttag tgaactatgg aatatcaaaa ttaatataaa attcactaaa gagagcgtac
     4741 ccaatggcac aattaccgct atatctgact tctgaaatca aagactttac tgtcggcacg
     4801 cccgaagttt tacaatcatt tttcgaatat gtcccttatg gcgtcgtctt tgaagacgac
     4861 ggcgacacag gctacttcta tgccgcttcg caagacggga ttctagatgc cttgcacatc
     4921 tacaatgttg aagatgtatc cgacaaacat atccccaatt atgtcttgat tttatgggat
     4981 gatgcctgca ccatagccgc attgtgtatc aacgactaca ttcatgccgt ctatgatttt
     5041 gtcgcgcagg caggatattg ccgcaacggc ttccctgaag cacaaggcga atggctgaaa
     5101 gtcgcagacc gcgtcttgga cgatgaattg ttggacaaaa tcctatcccg aaaacctaca
     5161 taacccactc aaaaggataa caaaatgccc ctactagaca gtttcaaagt tgaccacacc
     5221 cgtatgcacg cccctgccgt acgcgtggca aaaactatga ccacgcccaa aggcgacacc
     5281 attaccgtgt ttgacctgcg tttctgcatt cccaacaaag aaatcctgcc cgaaaaaggc
     5341 atccacacgc tggagcattt gttcgccggt tttatgcgcg atcacttgaa cggcaacggc
     5401 gtggaaatca tcgacatttc cccgatgggc tgccgcaccg gtttttatat gagtttgatt
     5461 ggtacgcctt ccgagcagca ggttgccgat gcgtggctgg cttcgatgca ggatgtggcc
     5521 aatgtcaaag accaaagcaa aatccccgag ttgaacgaat accaatgcgg cacttaccaa
     5581 atgcactcgc tcgccgaagc gcaacaaatc gcgcaaaatg tgctggcgcg caaagtggcg
     5641 gtgaataaaa acgaagagct gacgctggac gaagggctgc tgaacgccta atccgccaaa
     5701 aatgccgtct gaacaagggt ttcagacggc atttgccttt tccgttataa tccggggttg
     5761 tgcggggtcg ggttttaagc cggcatcgtc cttccctatt tttttctgtc ccttatcggt
     5821 tttaagcggg ttttttatgt ccaacagacc tacactcctc ctcgttgacg gctcgtccta
     5881 cctctaccgt gcgtatcacg cgatggggca aaacctgacc gcccccgacg gcgcgccgac
     5941 gggtgcgctg tatggcgtat tgaatatgtt gcgccgtttg cggtcggaat atccgcacga
     6001 ttattgcgcg gtggtttttg atgcgaaagg caaaaatttc cgccatcaaa tgtttgaaga
     6061 atacaaggcg acgcgcccgc cgatgcccga cgatttgcgc ccgcaggcgg aagcactgcc
     6121 ggatttagtg cgcctgacag gctggccggt attggtgatt gggcaggtgg aggcggacga
     6181 tgtgatcggc acgctggcga aacagggggc ggaacatggt ttgcgagtca ttgtttcgac
     6241 cggcgataag gacatggcgc agttggtgga tgagcgcgtt acgctggtga acacgatgag
     6301 cagcgaaacg ctggacattg aaggcgtgaa ggcaaaattc ggcgtgcgcc ccgaccaaat
     6361 ccgcgattat ctcgcgctga tgggcgacaa ggtggacaac gtgccgggcg tggaaaaatg
     6421 cggcccgaaa acggcggtga aatggctgga agcctacggt tcgctggctg gtgtgatgga
     6481 acacgcttcg gaaatcaagg gcaaagtggg cgagaatctg cgtgccgccc tgccgcgcct
     6541 gccgctgtcg tatgacttag taacgattaa aaccgatgtg gacttgcacg ccgagctttc
     6601 agacggcatc gaaagcctgc gccgtactac gccgaaatgg gcgcagctgg ttgtcgattt
     6661 caaacgctgg ggcttccgca cctggctgaa agaagcggaa tcaaacatga ataccggctc
     6721 gaccgatgat ttgttcggca gcgacagcat cggcgagcag gcggctttga atgcggaaat
     6781 gccgtctgaa aaacaagctg aaaaagccac cgtccccgaa aggctggatt atcaagccgt
     6841 taccaccgaa gcgcagtttg ccgctttgtt ggacaaactg gctcaggcgg acacaatcgg
     6901 catcgatacg gaaaccacgt cattagacgc gatgaacgcc gcgctggtcg gcatcagcat
     6961 tgcgttccaa gcaggggagg cggtttacat ccccgtagga cacagcctga ccgccgcgcc
     7021 tgaacagctt gatttacaat acgtattagg ccgtctgaaa ccgcatttgg gaaaccccgc
     7081 cctgaaaaaa atcgggcaaa acctcaaata cgaccaacac gttttcgcta actacggcat
     7141 cgccctgaac ggcattgccg gcgacgccat gctcgcttcc tacatcatcg agagccatct
     7201 cggacacgga ttggacgaat tgtccggacg ctggctcggc ttggaaacca ttacctacga
     7261 atcgctgtgc ggcaaaggcg cgaagcaaat cggttttgcc gatgtcgcca tcgggcaggc
     7321 gaccgaatac gccgcccaag acgccgattt cgccctgcgc ctcgaagcgc acttgcgcgc
     7381 gcaaatggac gaaaaacagc ttgaaatgta tgaaaaaatg gagctgcccg tcgcgcaggt
     7441 attgtttgaa atggaacgca acggcgtgca aatcgaccgc gccgaactcg cccgccaaag
     7501 cgcggaactc ggcgccgagc tgatgaagct cgaacaacaa gcctatgccg ccgcaggcca
     7561 gccgttcaac ctcaattcgc ccaaacagct gcaagaaatc ctgttcgaca aaatgggcat
     7621 ccccaccaaa ggcctgaaaa aaaccgccaa aggcggcatt tccaccaacg aagccgtgct
     7681 cgaacagctc gcgcccgact accccctgcc caaaatcatc ctgcaaaacc gcagcctggc
     7741 gaaactcaaa tccacctaca ccgacaaact gcccgaaatg atttcgcccc gggataaccg
     7801 cgtgcatacc acctacgccc aagccgtcgc cattacagga cgcttggcaa gcaacaaccc
     7861 caacctgcaa aacatcccca tccgcaccgc cgaaggccgc cgtgtgcgcc gcgcctttac
     7921 cgcaccgcaa ggcagcgtca tcgtttccgc cgactattcc caaatcgagc tgcgcattat
     7981 ggcgcacctc tccggcgaca aaaccctcat cgccgcgttc caaaacggcg aagacgtaca
     8041 ccgccgcacc gccgccgaag tgttcggcac ggcgcccgaa aacgtctcgt ccgagcaacg
     8101 ccgctacgcc aaaaccatca acttcggctt aatttacggt atggggcaat acggtttggc
     8161 aaaatcattg ggcatcgaca acctttccgc caaaaacttt atcgaccgct acttcgcccg
     8221 ctaccccggc gtcgccgaat acatgcggcg caccaaagaa caagccgccg cccaaggcta
     8281 cgtcgaaacc ctgttcggca gaaggctcta tttgcccgac atccgcaaca aaaacgccaa
     8341 cgcccgcgca ggagccgagc gcgccgccat caatgccccc atgcaaggca ccgcgtccga
     8401 cctcatcaaa cgcgccatga tagacgtgtc ccattggttg gattcagccg gcctgaaaac
     8461 caaattgatt atgcaggtgc atgacgaatt ggtgttggaa gtacccgaag ccgagctgga
     8521 tttagtcaaa gaaaaactgc cgcagataat ggcgaaagtg gatgaaggga tgttgaaagt
     8581 gccgctggtg gctgaggttg gcgtagggga gaattaggaa gaggcacatt gattgaaagg
     8641 tgttatatgc tatctttatt taaataaaat ttaatttttg gtatattttt tctaaatgtt
     8701 ccta
//
LOCUS       DAXHSJ010000081         8629 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000081 DAXHSJ010000000
VERSION     DAXHSJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.081"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(1..182)
                     /locus_tag="ACFM8U_001285"
                     /pseudo
     CDS             complement(1..182)
                     /locus_tag="ACFM8U_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            328..1032
                     /locus_tag="ACFM8U_001286"
     CDS             328..1032
                     /locus_tag="ACFM8U_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC6334522.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQTDASLEYTDFSVSRERRNEVIDQVSKDAILRFK
                     TRAEKLAGILGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     QGVEEISISVNGTVQF"
     gene            complement(1096..1662)
                     /gene="dcd"
                     /locus_tag="ACFM8U_001287"
     CDS             complement(1096..1662)
                     /gene="dcd"
                     /locus_tag="ACFM8U_001287"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC6334523.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(1728..2144)
                     /locus_tag="ACFM8U_001288"
     CDS             complement(1728..2144)
                     /locus_tag="ACFM8U_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFC6334524.1"
                     /translation="MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGGVGKDGLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(2206..3105)
                     /locus_tag="ACFM8U_001289"
     CDS             complement(2206..3105)
                     /locus_tag="ACFM8U_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC6334525.1"
                     /translation="MWFKQISFYPLNKEKLPEADILADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNIGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            complement(3136..4593)
                     /gene="der"
                     /locus_tag="ACFM8U_001290"
     CDS             complement(3136..4593)
                     /gene="der"
                     /locus_tag="ACFM8U_001290"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC6334526.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEEIL
                     ENFPEPEVEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(4743..5372)
                     /locus_tag="ACFM8U_001291"
     CDS             complement(4743..5372)
                     /locus_tag="ACFM8U_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC6334527.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(5373..6668)
                     /gene="hisS"
                     /locus_tag="ACFM8U_001292"
     CDS             complement(5373..6668)
                     /gene="hisS"
                     /locus_tag="ACFM8U_001292"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC6334528.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            complement(6786..7442)
                     /locus_tag="ACFM8U_001293"
     CDS             complement(6786..7442)
                     /locus_tag="ACFM8U_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6334529.1"
                     /translation="MMKFKYVFLLACVAVSLSYRLNAAPMFNDNPVVYGKIKVQSWKE
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLKKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLEAWQTREGNLAGKILAVVPKKAETISNKLFFTHHPKRQTEFAVEQIRQARAE
                     "
     gene            complement(7500..7691)
                     /locus_tag="ACFM8U_001294"
     CDS             complement(7500..7691)
                     /locus_tag="ACFM8U_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334530.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(7724..8260)
                     /locus_tag="ACFM8U_001295"
     CDS             complement(7724..8260)
                     /locus_tag="ACFM8U_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334531.1"
                     /translation="MEKIIDSKVRETNFNQAWRVIRAMQVDNISDEQVVIVRMSCYLE
                     KDEIFKNVERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(8428..>8629)
                     /locus_tag="ACFM8U_001296"
     CDS             complement(8428..>8629)
                     /locus_tag="ACFM8U_001296"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334532.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT         2065 a         2073 c         2133 g         2358 t
ORIGIN      
        1 tgcaaaaccg taagggtcgg tcaggatgct tgctgtttct ttccgcaagt agcggaacat
       61 cggtgcaagc acggcggttt tgatgccttt cctctggaat gccagtgcct gattttgaaa
      121 aaaatcccgt ccacatcctg ttcggattgc ggataccatg aggggatgac gaggacgtgc
      181 aagggttcgg gcatagtggg attccgtatc ggaaaggcgg ttattataag acagacgcag
      241 accgaatatt taaattgttg ccttacgcta atgcaattta gcgcgccgat atgctagatt
      301 ggcggtttta tgagtgagga tacggatatg ttgcgttcta ttttggcggc ttccctattg
      361 gcggtatctt ttccgacggc ggctgaagca ttgaattaca atattgtcga attttctgaa
      421 tcggccggtg tcgaggcggt tcaggataca atgtccgcgc gtttccaggt ggcggcggaa
      481 ggacgggaca aaaatgccgt caatgccgag tttgttaaaa aattcaacag ttttaacaga
      541 aaatcgaaaa acggcagctt taaaaccgaa ttggtatcgc gcagtgcgat gccgcgctac
      601 cagtacacca acggcagacg cattcaaaca ggctgggagg agcgtgcgga atttaaggtc
      661 gaaggtaggg attttgatgc gttgaaccgt tttattgctg atgttcagac ggatgcttcg
      721 cttgaatata ccgatttcag cgtgtcgcgt gaacgccgaa acgaggtcat cgatcaggtc
      781 agcaaggatg ctattttgcg ttttaagacg cgtgccgaaa aactggcggg cattttgggt
      841 gcgtccggtt ataaaatcgt caaattaaat ttgggacaca tcggcagcca tatcgcggga
      901 gggggagctg ctcaggcaaa aatgcttcgt gccatgccga tggcggcaag cgtcaatatg
      961 gagggtgcgg attctgccgc gcagggtgtg gaggaaatca gcatcagcgt caatgggacg
     1021 gttcagttct aaccacggat aaacaggtag atgccgtctg aaaccggatg atacggttca
     1081 gacggcattt atatttcagg ctttaggcag ggtaacgccg gtttgcccca tatatttgcc
     1141 gttgcggtct ttgtaggaag tttcgcagat ttcgtcgctc tcgaaaaaca ggacttgcgc
     1201 cacgccttcg cctgcgtaga ttttggcggg caggggggtg gtgttggaaa attcgagggt
     1261 aacgtagcct tcccattccg gttcgaacgg ggtaacgttg acgataatgc cgcagcgggc
     1321 gtaggtggat ttgcccaagc agacggtcag gacgttgcgc gggatgcgga aatattcgac
     1381 cgtgcgcgcc agtgcgaagg aattgggcgg gatgatgcag cagtcgtctt caacggtaac
     1441 gaagtttttc gggtcgaagt ttttgggatc gacgatggtg ctgttgatgt tggtgaagat
     1501 tttaaattcg tttgcgcagc ggatgtcgta gccgtagctg gacgtaccgt aggagatgat
     1561 gcgtttgccg tcggcttctt tgatttggtt cggctcgaaa gggtcgatca tgccgaattc
     1621 ttcgctcatt cggcgtatcc atttgtcgga cttgatgctc ataatgtttt ccttgtttct
     1681 tgcagtgttc ggacaaagca ttgggggatg ccgtctgaaa acggggctta tttgtttttg
     1741 ggcagcttca cttctttaat catgccgttt tcgcatttca taatgaagag ggtttcgccg
     1801 ttttgcgaga cggtaaatct gccgtgtgcc aagccttttt tgaacgtgcc cgagagtacc
     1861 atgttgcgga atttggtact gtcggaattg aacggttcga taaatatttc gcggttggcg
     1921 gcaacggtat aaacgccttg tccgtcgaat ttgccatttt taaacgaacc ggtataaccg
     1981 cgcccgtccc ggcaacgcca tatgcctttg cctgaaggaa gcccgtcttt tccgactccg
     2041 ccgtcgtagg tgcagccggg ttcttgatag gaagtcagaa cggcggccga agtggggagg
     2101 gcgaacatca tggcgggcag taggaatgcg agatgtttaa gcataagggt tattccattg
     2161 gattttggtt gacggtattt tgtcgtgaaa aagccgtctg aaaaatcaat cttgccagcc
     2221 gcccaaatag gaaaccagtt cttccaacat ggtgctgacg gattccgcca tcagaatttg
     2281 cgaggcgaag gcaaggccgg cggcatcgtc gccgttgctt tcggcttctt cctgcaatac
     2341 gtcgaggtat tggatgcgct tgagtgtgaa gtcttgagtg aggataaagg caatttgttc
     2401 gcgccacacc aagcccagtt gggtaacggt tttaccgttt ttgacgtgtt gaaccacttc
     2461 gtcggcggtt aaatcttgtt tggatacttt gacgacggga acaatatcgc ccgtaccttt
     2521 gagttcgcaa tcgctgtcta attcaaaacc gccttcgcaa tgcccttgca acagccagcc
     2581 ggtcatcaag gaagagggcg attgcttggt attcggcagc gaggcttcca aaccgcccaa
     2641 agcttcgcgc agcttggtca ggatgttttc cgctttggcg gaagccgcgt tattgacgag
     2701 caggtagccg tggcgggtgt taaacactgc ttctgtacgg ctgctgcggg taaacgctcg
     2761 gggcagcagg tcgtctgtaa tttgctcttt aagctcttgt ttttctttgc ggccgatatt
     2821 gcgtgcctca ttgttttgga tttccgctac cttctcttcc aaaatatcgc ggatgacgcc
     2881 ggcaggcagg actttttcct cttttttcag ggcgacgcgc aaggtaaagt cagcagggaa
     2941 aacgagttcc ggggagaatg aaaccggtgc ggtaaagcct tcgctgaacc agtctaagcc
     3001 ttggcaatgg gtaaattcag cttcagcaag tttgtcggca agtatgtccg cctcaggcag
     3061 cttttctttg ttgagcggat aaaaactaat ctgcttgaac cacataatgt ttcctattgt
     3121 ttgaaatgtc gagaattatt tgctgaattg ttttttcaca ctgactttgg ttttcttctt
     3181 gaagcggttt ttctcttctt tgcggccttc gcgtttctca atacggttgc tcaggctgac
     3241 gcggcgcagc ggttttttct tcggtttgtc ttccgcattt tcatacgggt tttccgaaac
     3301 attgtattga atccgcaacg gcgtgccttg caggttgaag gctttgcgga acgtttgggt
     3361 cagatagcgc gtatagctgt cggaaatcgc gtgcagcgaa ttgccgtgta ccacaattac
     3421 gggagggttc atgccgcctt ggtgggcata acgcattttc ggacgcacca agccggcacg
     3481 cggcggttgc tgacgctcga tcgcgctttg taatacgcgc gtgattttcg gtgttggcat
     3541 cttaatcatc gccgcgttgt aggcagcctg aatgctgtcg aacaatccgt ctatgccgcg
     3601 ttctttcaat gcggaaataa agtggaattt agcaaaatca aggaaataca gtttgcggtt
     3661 gatatcgcgt ttcacttgct cgcgacgttc ttcgctgatg ccgtcccatt tattgaccgc
     3721 caccaccaaa gcacggcctg cctccaaagc gaaacctgca atcgtcgcat cttggtcggc
     3781 gatgtcctgc tgcgcgtcca ataccaaaac agcgacgttt gccgcttcaa ccgcctgcat
     3841 ggctttgata acggagaatt tttccactgc ttcatccact ttgccgcgac ggcgcacacc
     3901 tgcggtatcg atgatggtaa acggtttgcc ttcgcgctcg aaatcgatat ggatactgtc
     3961 gcgcgtcgtg ccggccatat cgaaggcaat cacgcgctct tcgccgagaa tggcgtttac
     4021 cagcgtggat ttgccgacgt tcggacgacc gatgacggca aaaacgggat gttttgcatc
     4081 ggcttcttcg acttccggct cggggaagtt ttctaaaatt tcttcaatca ggtaatacac
     4141 gccgtcgccg tgcgcaccgg aaataacata agggtcgccc aaagcaagtt cgtagaactc
     4201 ggcggcaagc acggccctgt tgccgccttc gcctttgttt accgccagat aaacagggcg
     4261 cgggctttgg cgcaaacggt cggcaatgat tttgtcttgc ggggtcagac cggtgcggcc
     4321 gtccaccaaa aatacgacgg catcggcttc atcgacagcc tgcaaggttt gttttgccat
     4381 ttcgtgcaaa atgccgctgt ccacaaccgg ctcgaaacct ccggtatcga tgaccaaata
     4441 aggtttgctg ccgactttgc cgtgtccgta atggcggtcg cgcgtcagac cgggcaggtc
     4501 gtgtacgagc gcgtctttgg tgcgcgtcaa acggttgaat aaagtagatt tgccgacgtt
     4561 ggggcggccg acaagcgcga tggttggttt catgattggg tctttctgtg tcaagtgccg
     4621 ttcgggagaa ctgaacacaa gcaggtgtcc gttgggacac ggcagatggt tttacgggaa
     4681 ttgccgtagg atagtgttgt ctgaaatgcc gtctgaagag agggtggcat ttcagacggc
     4741 atttatttca gcgaatcgag tttcatttga accaattcac gaccgacaga atcttgaggc
     4801 attttttcta aagcctgacc gtagtttttt aaggcttcct ggctttttcc ctgtgcggca
     4861 tagacatcgc ctttggtttc catcagcagg ggggagaagt ccgcttcaac cggcgtgtcg
     4921 agcgcggcaa gcgcggcatc gtattttttt tgttgcaaca acacaacgcc cagacgctgc
     4981 gccgccaacg cttgaatcag gctgtctttt tggttggaca acacccattt caaatggcct
     5041 tcggcaacat cgtaacgctg cgcgtcaaat tcggttgccg ccgccatcag cgtggcttgg
     5101 gcggcggaaa tagaatgcgg gtagctttgt tggagtttgg tcaattcggc attaatttcg
     5161 ctttgcgggg ctttgttttg cgccttttcc acgatgtttg ccagcaccgc cgccgcttcc
     5221 tgattttggg aagccgcacg gttttggtaa accgtgtatc ccaagtagcc gagtgctgcc
     5281 aaaatcagca gggcaaacag ccatttgccc gtggttttcc aaaaatattt aaagttgtct
     5341 aactcttgtt gttcttcgag atgggctgcc atttatgcgt tcttccattg ttgtaaagta
     5401 tgggttaaat cctcggcggc gacagtttgc tgaccgtgtg cgccgttcat gtctttgagc
     5461 gtaaccgtac cgttcgccag ttcgtcttgc gcgacaatca gggcaaagcg cgcgccgctg
     5521 ttgtcggctt ttttcatttg cgctttcagg ctttgatagc cggaatgctg cattacattg
     5581 aaaccttgcg cgcgcaaggc ttgcgcgtat ttcatcacct gcaagtccgc cctttcgcct
     5641 tggtgcattg catagacatc aggcgcagcg ttcacttcca gcgagccgta ttcgctcacc
     5701 aaaagcagca gccgttcgat gcccatcgca aagccgatgg acggcgcagg tttgccgcca
     5761 agttcctcaa tcaaaccatc gtaacggccg ccgccgcaca cagttgcctg cgcgccgagt
     5821 ttgtcggtcg tccactcaaa aaccgtctga ttgtaataat ccaaaccgcg aaccaagcgc
     5881 ggattttcaa tatattgaat acccaaacca tccaacatcg ccttgaagcg tgcatagtgg
     5941 ttttgcgaag cctcgcccaa gtaatccacc aaacgcggcg cggcgttgca gatttcctgc
     6001 aaatcagggt ttttcgtatc taaaacgcgc aaaggattgg ttttcagacg gcgtttgctg
     6061 tcttcatcca atttatcttc ataacgggtc agatattcaa ccaatgccgc acggtgtgcc
     6121 gcgcgttcct cacggttgcc caagctgttg atttccaaag tcaggtattc gcgaataccc
     6181 aatttttccc acaagtcggc agacatcgcg atgatttccg catcaatatc cggaccttca
     6241 aaacccaaag cttcgatacc gacctgatgg aactgacgat aacggccttt ttgcggacgc
     6301 tcgcggcgga acatcggccc catataccac agcttttgcg ggctgttgta gagaaggttg
     6361 tgttcgacca ccgcacgcag acaagaagcc gtaccttcag gacgcaggct caaactcaaa
     6421 gaatcgtttg aatcggaaaa ggtgtacatt tccttgccga ccacatcggt ttcttcgccg
     6481 atggagcgga caaacaaacc ggtttgctcg acaatcggcg tacggatttg ctggtagccg
     6541 taagcgcgcg tccaacggtt taccgtatct tcaaacgcct gccaaaacgc agccgtcagt
     6601 ttgaaatctt tttgttcgac aggtaaaagg tcgttcatac cttttacaga ttgaattttt
     6661 tgtgccattt caatatggga taagcgaaaa tcaaatagtt gggcattata gctgattttt
     6721 aagggtttgt gaggttggga gtaaatcaaa aggccgtctg aaacagtttt cagacggcat
     6781 ttgacttact ctgcacgtgc ttgcctgatt tgttcgactg caaactccgt ctgccgcttg
     6841 ggatgatgtg tgaaaaacaa tttatttgaa attgtctcgg cttttttcgg cacgaccgcc
     6901 aaaatcttac ctgccaaatt tccctcacgg gtttgccaag cctccaaaaa ctgcgccctg
     6961 ctcattgaaa catgccccag cgacgggtcg gcaagcaaaa ccgtattgcc gtctatcccg
     7021 cgcaataccg agaaatgatc atccttgcgg tatttcagat acacgatgac ggggattttc
     7081 aactgcgcga gctgctcgaa agacagggca tagcctttcg cttcaaaacc caaatcaggc
     7141 ataatgcgcc gcatatcctc aaacgacgcg cgcatctgct ccttatccag ctttttcaac
     7201 acttcttctt ccgtcagcgt ttgcccgtaa aaattgttca aaagcgtcgc caccgaagcc
     7261 gccccgcagg aaaaatccaa atcctgcttt acaatattga aatcgcgcct ctctttccaa
     7321 ctctgcactt tgatttttcc gtaaacaaca ggattatcgt taaacatcgg cgcagcattc
     7381 aaacgataag ataaagaaac ggcaacacac gccaacagaa aaacatattt gaacttcatc
     7441 atattttcca cataaagggc agcctgaaaa tctttcaggc tgcccttgtc aaattattcc
     7501 tagctttcgg cttttttggc aaaccaaaca atccgattac ccgcataata ctttccattt
     7561 attgaaatcc gacaagccgc gcccaaaaaa tgccatgcac tgtcgatttc cgcagcaatc
     7621 tttgtaccgt tttcttcaaa ttccaaatat tcacccaata ataaacttga aacagaacgc
     7681 gtgggcagca agtgcccacc ctacgcttat tcaaataatt tgattaaata aagaataaag
     7741 aaaatgcatc aggaacaaaa ttataatctg ccacctgact cacaccgctt ttaaagtaag
     7801 gggcatcaaa atcaaaaccg caaaaaaaat aatttttgca ttgattttta atagatttaa
     7861 aattcgaata tagtgtttct ccagatttaa aaagtttatc tttatctata tatttgatgc
     7921 tttccctatc caaaataaat atttcaaaca ttaaaaaatc attacatgac caagccaaat
     7981 cataaatttg attcaaatgg gtatcataaa gataaaaata ataaggtttg ggaacaggta
     8041 aaatatttaa tggaaaagga ggactaattt tcttaatatc tgatgactga tatccatatc
     8101 tttctacatt cttaaaaatt tcatctttct caagataaca agacattctg acaatcacaa
     8161 cctgttcatc ggatatatta tctacttgca tggcgcgtat aacacgccat gcctgattaa
     8221 aattagtctc ccttacctta gaatctatta tcttttccaa agatgaaaat gccccttatc
     8281 ttgaactctc cagttagaac ctagaagttt cgtacctctt aaatgtgtat taatatttct
     8341 tatagtttca ttaaaatgcc acgcgctgcc gatttcaacg gtaattttcg taccgctttc
     8401 ttcaaattcc aggtattccc ccattagcta acgcaaagaa gcagacgcca tttcggcttc
     8461 gttatgataa acccgccttc cgttgatata gacttccgcc cctgtccgct ccaaattcca
     8521 aaaattccgt actgaaattt ccatatcccg atattgtgca gaccatgttt tttcgaaggt
     8581 tttcataaaa tttcctatac ctgtccaatc ggcacatatc aattgcatt
//
LOCUS       DAXHSJ010000082         8619 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000082 DAXHSJ010000000
VERSION     DAXHSJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.082"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(137..790)
                     /locus_tag="ACFM8U_001297"
     CDS             complement(137..790)
                     /locus_tag="ACFM8U_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFC6334533.1"
                     /translation="MWEILFRHQDFVAINKPQGISVHRSDGEVSLTATLAAQLGVEKV
                     WLLHRLDKQTGGILLFALNSQSAAVLAAQFAERKMKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNMENIAVTQFFSIRISEKMRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDRLYGGTESETTFLYAWKIQFTYQQQEIEITAPLKNAWQAENISHALEEFYMAKAV"
     gene            complement(826..1338)
                     /gene="coaD"
                     /locus_tag="ACFM8U_001298"
     CDS             complement(826..1338)
                     /gene="coaD"
                     /locus_tag="ACFM8U_001298"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC6334534.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETVKRYVPPAVYQ
                     KMIAEHHNNA"
     gene            complement(1462..2145)
                     /locus_tag="ACFM8U_001299"
     CDS             complement(1462..2145)
                     /locus_tag="ACFM8U_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFC6334535.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            complement(2302..2568)
                     /locus_tag="ACFM8U_001300"
     CDS             complement(2302..2568)
                     /locus_tag="ACFM8U_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC6334536.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(2641..3111)
                     /gene="rlmH"
                     /locus_tag="ACFM8U_001301"
     CDS             complement(2641..3111)
                     /gene="rlmH"
                     /locus_tag="ACFM8U_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC6334537.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKTWQQNGEHVCFV
                     IGGADGMTDRLKQQARIMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            complement(3165..3551)
                     /gene="rsfS"
                     /locus_tag="ACFM8U_001302"
     CDS             complement(3165..3551)
                     /gene="rsfS"
                     /locus_tag="ACFM8U_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC6334538.1"
                     /translation="MNEQELQDLQKMVEVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            complement(3610..4215)
                     /gene="nadD"
                     /locus_tag="ACFM8U_001303"
     CDS             complement(3610..4215)
                     /gene="nadD"
                     /locus_tag="ACFM8U_001303"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC6334539.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDTVVFLPAGGPYH
                     KDAASTSAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            complement(4212..4811)
                     /locus_tag="ACFM8U_001304"
     CDS             complement(4212..4811)
                     /locus_tag="ACFM8U_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC6334540.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGIDLSEGMETS"
     gene            complement(4840..6381)
                     /locus_tag="ACFM8U_001305"
     CDS             complement(4840..6381)
                     /locus_tag="ACFM8U_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6334541.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRCAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSADESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTAYRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGADNYARAMVLTLLLSAFALGIFLLLDGGEGGK
                     QTETL"
     gene            6620..8005
                     /locus_tag="ACFM8U_001306"
     CDS             6620..8005
                     /locus_tag="ACFM8U_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC6334542.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            8025..8543
                     /locus_tag="ACFM8U_001307"
     CDS             8025..8543
                     /locus_tag="ACFM8U_001307"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC6334543.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT         1966 a         2509 c         2257 g         1887 t
ORIGIN      
        1 gaactgcttc atcaccgccg cttcgtcagc agcgaagaag cgaactatac gcttgaagag
       61 atttgcggtc aaccgttttc ttaaaaaatt tttataaaaa tatttatcat attgtttata
      121 aattaattta tatttattaa actgcttttg ccatataaaa ttcttccagc gcatgagaga
      181 tattttcagc ctgccatgca tttttcaaag gtgcagtaat ttcgatttct tgttgttgat
      241 aggtaaattg tattttccat gcatacagga acgtagtttc ggattctgtc cctccgtata
      301 agcggtcccc caaaatagga ctgcccagac ttttcattgc cactctcagc tgatgtgtct
      361 taccggtatg aggctcaaga atgaacagcc gcattttttc actgatacgt atactgaaaa
      421 actgggtaac ggcaatattt tccatattgc gcgtcagctt ccacattccg cgtctggatt
      481 tttccatgcc gccttttatc catccttgtt ttttggacgg tttgcggtcg gacaaagcta
      541 aataggtttt cttcattttc ctttcggcaa attgtgcggc aagaacagcg gcactttggg
      601 aattaagggc aaacagcaat atgcctccgg tctgcttgtc caacctgtgc agcaaccata
      661 ccttttctac gcccaactgt gccgcaagtg ttgccgtcag actgacttcg ccgtcggaac
      721 ggtggacgga tatgccttga ggtttattga tggcgacaaa atcttggtgt ctgaataaga
      781 tttcccacat atttgaacct gaatatattt gacagaagct ttctgtcagg cattgttgtg
      841 atgttctgca atcatttttt ggtacacggc cggcggcaca tatcttttga cggtttccgt
      901 ccagccttcc ggcccgacca aacctttgac catagtggac gacacttcgg cgatttcgcg
      961 cggcggcatg aggaatacgg tggatatttc gggggcgagg tcgctgttga tatggcgcat
     1021 ggaacgttcg tattcgtaat ccgaagcaga acggatgccg cgcacgatga atcctgcatc
     1081 tacctcacgg gcgtaatgca ccagaaatcg gttttcaaat acatcggttc tgacgttggg
     1141 aaacatttta gtaatatcgc acaacatatc ctgcctttca gcgacggtat aggtgctgcg
     1201 tttgtcgggg ttaatgccga tggcgacgat gagttcgtca aacatagatt gcgcctgccg
     1261 tatcatccac agatgcccca atgtgggcgg atcgaaactg ccggcataaa cggcgcggcg
     1321 cggtgtattc ggtaacattt gattcctccc ggcttcatag tcggctgtgt gttggtgcgt
     1381 gtgcatccgt attgtatgcc caaagtaaaa tgccgtctga agcattttca gacggcatag
     1441 tcggacggcg ttttaccggc atcaatcctc gccgtttaaa gacaacagga tgttcagcag
     1501 gctgctgaag atgttgtaaa gcgagataaa cagtgtcagt gccgcgctga tatggctgtc
     1561 ttcgccgccg tcgatgacgg tgcgtacctg ccacataatc attaaggaac tgaacaagac
     1621 aaaaccggcg gaaatggtca gggcgagtgc gggaataccc aaaaacagat tggcaaccac
     1681 ggcgaccatc agaatgaccg cacctacggt caggaagcgt ccgagcgcgt tcatatcgag
     1741 ccgggttcgg cgcgccaagg cggacatcgt taaaaagacg gcggcggtca tcgcggcggc
     1801 aatgccgacg attttcgcac cgtcggcaat atggagcgcg tattgcagca cggggccgat
     1861 caatacgccc ataccgaatg tgaataccat cagcagggta acgccggtat tgctgtaacg
     1921 gtttttctcg atgaagtgga tcataccgta gaaaaacgcc aacacgacgg caaaccctat
     1981 ccagcgcgaa ccgaaggcgg cgtaaaaatt gaaaccggca ttggcggcaa gtgccgcgcc
     2041 tgcggcagcc ggaatgaatg aaaatccgag caggcggtag gttttctgca ggacggtgtt
     2101 tttagaaacc gtatgcgcgg tgtagtcgta aacgtcgtgt tgcatatcat ctgctcctga
     2161 aagcgcggtt gggaataatg ggggatttta acattgccca atgtcaaaat ttgtccggtt
     2221 gcgtgaagat aaagttgtcc ggcgtatttt aaaggccgtc tgaagcagtt tcggacagcc
     2281 tgtgttcaaa acggaaaacc gttattgcgg aacgtatccc tgaacggcat ccgcgccgtc
     2341 gccgaagaaa tactgctcca tctgctgagc caggtattcg cgcgcgcgcg gatcggcgag
     2401 gcttaaacgg ttttcgttaa tcagcatcgt ttggtggcgc gtccacgccg cccacgcttc
     2461 ttgcgatacg ttttcaaaaa tgcgcttgcc caattcgttg ggaagcggtg gaaatttcat
     2521 gccttcggct tctttgttga gcttgacgca gaataccatg cgtgccatag cggattcctt
     2581 tgctgtgttc agaaataacg gggtaatttt aaccgattag ggatacggac aaaagccttc
     2641 ttattcccga tgatagggat ggttgtgcag gatggaggcg gcgcggtaga gctgctcggt
     2701 cagaaggacg cgcaccatcc cgtgcggcag ggtcaggctg gacaggcgca tcattatgcg
     2761 ggcttgttgt ttgaggcggt cggtcattcc gtccgcgccg ccgatgacga agcagacgtg
     2821 ttcgccgttt tgctgccaag ttttgaggtg ttccgccagc tcgacggagg tcggtgcttt
     2881 gccgcgttcg tcaaggacga cgaggaacgc gccttgcggt atggcttcga ggatgcgttt
     2941 ttcttccgcc gccatacctt gggcggcatt cacgcccgcg ccgcgttttt cgggtttgat
     3001 ttctttgagt gcgtaggcga cgtcgcgtcc gaagcgtttg gcgtattcgg cgacggcctc
     3061 atcaacccag cgcggcattt tggtgccgac tgccaaaacg gtaatattca atgctttctc
     3121 ccttacagga aaatgccgtc tgaacattca gacggcatcg ggaatcagtc tgcagcgtgc
     3181 cacggcttct gcattccggc gtggaaactc ggtttctcgc cgccccagag ggtgtcgatg
     3241 tcgtagaagt cgcgcacggc agggagcatg acgtggacaa cgaggtcgcc tgcgtcaacc
     3301 agcgtccatt cgccgctgtc tccttcggta ctgaggattt caaaaccggc ttctttcaaa
     3361 tcgacggcaa cgttgttggc aagggctttg acttggcgcg tactgtcgcc gctggcgata
     3421 atcattctgg caaacagcga agttttgtct tgcgtttcga gaacggaaat gtctttggct
     3481 ttgatgtctt cgagggcgtt gacggcgacc tcgaccattt tttgcaggtc ttgcagttct
     3541 tgttcgttca ttatgttcct aacgggatgt tttcagacgg cattatagcc gtttcttact
     3601 gatttgactt tatttttcat acaaaccgtg ttcgcggatg tagcgtgcgg cggcaggcgg
     3661 gatgccgtct gaaacgcctt ggccggcaag gttgcggcgg atttccgttg acgacacatt
     3721 atgcatcggg gcggacaaga tgcggacgct gccgtcctga agggacttgc ccagccacgc
     3781 gtgcagttcg cgcggggttt ggtgcaggct gtcgccctgc cgcatggcga cggcgatatt
     3841 ggtttcgcgc acgagcatct gccatttttt ccatgtgtgc agcttcatca ggctgtcgct
     3901 gcccatcagc caccaaagtt gcgcggatgg gaactgctgg cggaagattt ggacggtatc
     3961 aaaagtatag gttgcacctt ctcggacgat gtcgcaatcg ctgacggcaa aacgcgcgtc
     4021 ttctgccgtc gccagttcga ccatggcaag gcggtcggcg gcggaagtgg aggctgcgtc
     4081 tttgtgatac gggccgcctg ccggcaggaa aacaaccgtg tccaacccga tttcatcggc
     4141 aaaggcacgg gcgatatgaa gatgcccgtt gtgtatcggg tcgaaagtgc cgccgaacag
     4201 tccgattttc ttcatgatgt ttccattcct tccgataagt ctatgccgtc tgaaacactg
     4261 ccgtccacaa ccagcgtcag tttgccggta acgggatgga tgaccagtcc gtgaacggcg
     4321 atatggcgcg gcatcagcgg atggttacgg ataaggtcca ccgtgtggcg cacgctgtct
     4381 tcgacgttgt cgaaaccggt cagccagccg tcgaggtcga taccggcata acgcagggtt
     4441 tcgatacggt cttcgggaat ccggctttcc cggacgcgcc cgaggaattc ttcggcattc
     4501 agcccctgca taccgcaatc gtgatgggcg atgaccataa tctctctgac cttcaattca
     4561 aacacggcaa ccaaaagact ccgcatcacc gaaccccacg ggtgcgtaac cagcgcgccg
     4621 gcatttttaa tcagcttggc atcgccgttt ttcaaaccca acgcgtcggg cagcagcccg
     4681 ataatccgcg catccataca ggacaaaact gccagcccgc gttcggggta tttgtcggta
     4741 aagtattttt catattcgcc cgactcgaca aactgccggt tatgggcaag gatgttatcc
     4801 aactcgctca ttttgccgtc ctctgaaaaa gggttcacat tataacgttt ccgtctgttt
     4861 tccgccttcg ccgccgtcca acagcaggaa aatgcccagc gcgaacgccg acaacagcaa
     4921 tgtcaacacc attgcccgcg cgtaattgtc cgcacccgcg cgtcccaaat aggcataaat
     4981 caaagtcgtc agcgtctgcc attccggacg cgacaggaac aatgtcgccg caaattcgcc
     5041 cacgcaggtt gccgccgcca aagtcagacc gcgccgcaac gccggtttca aaagggggaa
     5101 cgtgatgcgg tatgccgtct gaaagccgtt tgcacccaaa cccgccgccg ccctgccgta
     5161 atccggcggc agtgcatccc aggctgataa aacatctttt gccacaaacg gatacgccag
     5221 cagcgcatac atcgccagca gcagcggcaa cgaagccgtc cactgcggat aaagcagcag
     5281 cacgcccgcc gaaacacaaa ccggcgacac cataaacggc aaaaacatca gcccgcgcat
     5341 ccacgccgac cgccgcgccg ccgccgcata caccacaccc aaaaccgccg ccgcatacac
     5401 cgccgccgcc gagaagcgca aagtattcca caccgcctgc cacgtttcac tttccattaa
     5461 cacacgccac gattcgtcgg ccgaccacgc tttcacaaca attgccaaca aaggaaacag
     5521 gcagcacaca gacaacaccg ccgccgcaaa cgccagcagc acatattccc cgaccgactg
     5581 cggcggcgac ggcatcacag gggaaaccgc cttatccgaa accgcgcacc tgccgaacca
     5641 cgcatacagc aaccctgccg ccgccgttac ccccaacacc agccacacca gcaccgaagc
     5701 aaccgccata tcgagttcga acatgaccaa ctggtaaatt tccacttcga ccgtggcata
     5761 acggctgccg cccagcagca atgccagccc gaaccccgaa aaacaataca ggaagacaag
     5821 gcacacgccg ccggcaagcc acgggcgcaa aacgggcatt tcaatgtccc aaaaccgccg
     5881 ccacgccccc gcgcccaacg tccgtgccgt ctgaagccgt gccgcaggca cttgcacaaa
     5941 cccctgatac gccgccctga ccaacacagg aaggttgaaa aacacattgc cgtacaacaa
     6001 cagatacggc gtatcctgcc gtccgcgcca caacaggccg tccgccccaa acagagccag
     6061 cacgcccacg cccgccacca acgtgggcat cacaaaaggc agcatcagca ggcgcagcac
     6121 caaagcccgc cccggaaacg ccagccgcgc cagcacccac gcgacaggca cgcccaaagg
     6181 cagcaccagc acacaggttg ccgctgcctg aaataccgtc cacgccaaac gtttgagcat
     6241 ataggcatcc gacagcaccg cgcgccacgc caaaccgtca tacgccgcca ccgcccacaa
     6301 aggcgcaacg accattaccg ccaaaaaagc cgaaggcagc agggcaaaag caccccatac
     6361 cgcccaacgc cgtccatcca tcgccttccc cacttgaaac actgatgttg cgattgtacc
     6421 caaaagcccc acataccgta tatttcaatc cgactacata ccgtatccgc cttcctcccg
     6481 ccgtctgaaa taaatcgttc aaataaacag gaatataact tcagacaaac aacttaccgc
     6541 ccgatttgtg ctatcgtttt cgcacaactt aaaaaaacct gacaattttg tacttttatt
     6601 acagagaaag gctttacaaa tggacggctg gacacagacg ctgtccgcgc aaaccctgtt
     6661 gggcatttcg gcggcggcaa tcatcctcat tctgatttta atcgtcaaat tccgcatcca
     6721 cgcgctgctg acactggtca tcgtcagcct gctgacggct ttggcaaccg gtttgcccac
     6781 aggcagcatt gtcaacgacg tactggtcaa aaacttcggc ggcacgctcg gcggcgtggc
     6841 gcttctggtc ggcctgggcg cgatgctcgg acgtttggtc gaaacatccg gcggcgcaca
     6901 gtcgctggcg gacgcgctga tccggatgtt cggcgaaaaa cgcgcaccgt tcgcgctggg
     6961 cgttgcctcg ctgattttcg gcttcccgat tttcttcgat gccggactaa tcgtcatgct
     7021 gcccatcgtg ttcgccaccg cacggcgcat gaaacaggac gtactgccct tcgcgcttgc
     7081 ctccatcggc gcattttccg tcatgcacgt cttcctgccg ccccatccgg gcccgattgc
     7141 cgcttccgaa ttttacggcg cgaacatcgg ccaagttttg attttgggtc tgccgaccgc
     7201 cttcatcaca tggtatttca gcggctatat gctcggcaaa gtgttggggc gcaccatcca
     7261 tgttcccgtt cccgaactgc tcagcggcgg cacgcaagac aacgacctgc cgaaagaacc
     7321 tgccaaagca ggaacggtcg tcgccatcat gctgattccc atgctgctga ttttcctgaa
     7381 taccggcgta tcggccctca tcagcgaaaa actcgtaagt gcggacgaaa cctgggttca
     7441 gacggcaaaa ataatcggtt cgacaccgat cgcccttctg atttccgtat tggtcgcact
     7501 gtttgtcttg ggacgcaaac gcggcgaaag cggcagcgcg ttggaaaaaa ccgtggacgg
     7561 cgcactcgcc cccgtctgtt ccgtgattct gattaccggc gcgggcggta tgttcggcgg
     7621 cgttttgcgc gcttccggca tcggcaaggc actcgccgac agcatggcgg atttgggcat
     7681 tcccgtcctt ttgggctgtt tccttgtcgc cttggcactg cgtatcgcgc aaggttcggc
     7741 aaccgtcgcc ctgaccaccg ccgccgcgct gatggctcct gccgttgccg ccgccggctt
     7801 taccgactgg cagctcgcct gtatcgtatt ggcaacggcg gcaggttcgg tcggttgcag
     7861 ccacttcaac gactccggct tctggctggt cggccgcctc ttggacatgg acgtaccgac
     7921 cacgctgaaa acctggacgg tcaaccaaac cctcatcgca ctcatcggct ttgccttgtc
     7981 cgcactgctg ttcgccatcg tctgacagac ggaaaggata gtaaatgact acgcattttg
     8041 tcgttatggg cgtatgcggc tgcggcaaga ccaccgccgc gctgtccctg cagaaacacc
     8101 tcggtcaatg tccctatgcc gaaggcgacg agttccacac ccaagccaac cgcgacaaga
     8161 tgggcgcggg tattccgctg accgatgaag accgctatcc gtggttgggc aatctgcgcg
     8221 actggatgac gcaacaggcg caaaacggtg cgaaccacac catcgtaacc tgttccgccc
     8281 tcaaacgcgg ctaccgcgac attctgcgcg gagccgaagg caaagctgcc ttcatccacc
     8341 tcagtccgcc gcaagacatc aacctcgagc gcatgatgtc gcgcaaagga cattacatga
     8401 aagcagggat gctcgattcg caactggaaa tcctcgagga actgggcgaa ggcgaatacg
     8461 gggtcaaaat cgccaacccc ggcacgcccg aagcggtcga agccgatatt ctgaactggg
     8521 ttgcctcgga aaacctgctt tgaagcaata tgccgtctga agcccgacac aggatgggtt
     8581 tcagacggca taaacatcgg gaacagaatg gattacatt
//
LOCUS       DAXHSJ010000083         8604 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000083 DAXHSJ010000000
VERSION     DAXHSJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.083"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            77..1246
                     /gene="prpF"
                     /locus_tag="ACFM8U_001308"
     CDS             77..1246
                     /gene="prpF"
                     /locus_tag="ACFM8U_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HFC6334544.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDLYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1659..2858
                     /locus_tag="ACFM8U_001309"
     CDS             1659..2858
                     /locus_tag="ACFM8U_001309"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6334545.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGTVGMLLNELEKHELHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            2966..3586
                     /locus_tag="ACFM8U_001310"
     CDS             2966..3586
                     /locus_tag="ACFM8U_001310"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6334546.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKVDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(3713..4156)
                     /locus_tag="ACFM8U_001311"
     CDS             complement(3713..4156)
                     /locus_tag="ACFM8U_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC6334547.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLATPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4330..4698
                     /locus_tag="ACFM8U_001312"
     CDS             4330..4698
                     /locus_tag="ACFM8U_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6334548.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            4743..5897
                     /locus_tag="ACFM8U_001313"
     CDS             4743..5897
                     /locus_tag="ACFM8U_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6334549.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(5954..6826)
                     /locus_tag="ACFM8U_001314"
     CDS             complement(5954..6826)
                     /locus_tag="ACFM8U_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC6334550.1"
                     /translation="MPILNHIALIGVGLIGGSFILDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            6911..7723
                     /locus_tag="ACFM8U_001315"
     CDS             6911..7723
                     /locus_tag="ACFM8U_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC6334551.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
     gene            complement(7752..8561)
                     /locus_tag="ACFM8U_001316"
     CDS             complement(7752..8561)
                     /locus_tag="ACFM8U_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6334552.1"
                     /translation="MQPPKNLLFSSLLFSSLLFSSLLFSSTAQAASEDGSRSPYYVQA
                     DLAYAAERITHDYPKATGANNTSTVSDYFRNIRAHSIHPRVSVGYDFGDWRIAADYAS
                     YRKWNNNKYSVNTKEVLRNQGNWQELKTENQENGSFHASSSLGLSAIYDFKLNDKFDK
                     FKPYIGARVAYGHVKHQVHSVRKETTTTFSKAGAGATEPGKIILGPISKPAYHESHST
                     SSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYRF"
BASE COUNT         1857 a         2312 c         2509 g         1926 t
ORIGIN      
        1 gctttggtcg gagtggcagc ctaatccact ataaaaatcg tgggcagagc ccacgctaca
       61 taaggagaat ctagaaatgc cgcaaattaa aattcccgcc gtttactacc gtggcggtac
      121 atcaaaaggc gtgtttttca aacgttccga cctgcccgag gcggcgcggg aagcgggcag
      181 cgcacgcgac aaaatcctct tgcgcgtact cggcagcccg gatctctacg gcaagcagat
      241 agacggtttg ggcaacgcca gctcgtccac cagcaaggcg gtgattttgg acaagtccga
      301 acgcgccgat cacgatgtcg attacctttt cgggcaagtt tccatcgaca aaccttttgt
      361 cgattggagt ggcaactgcg gcaacctcac cgccgccgtg ggcgcatttg ccatcgagca
      421 aggcttggtc gataaaggca agattccttc agacggcatc tgcacggtca aaatctggca
      481 gaaaaacatc ggcaaaacca ttattgccca tgtaccgatg caaaacggcg cagttttgga
      541 aacaggcgat tttgagctcg acggcgtaac gttcccggca gccgaagtac aaatcgaatt
      601 tcttgatcca gccgacggtg aaggcagtat gttcccaacc ggcaatttgg tcgatgaaat
      661 tgatgtgccg aatataggcc gtttgaaagc cacgctcatc aacgcgggca ttccgaccgt
      721 tttcctgaat gccgccgact tgggctacac gggcaaagag ttgcaagacg acatcaacaa
      781 cgatgccgcg gctttggaaa aattcgagaa aatccgcgct tacggtgcgc tgaaaatggg
      841 tctgatcagc gacgtatccg aagctgccgc ccgcgcgcac acgccgaaag tcgccttcgt
      901 cgcgcccgcc gccgattaca ccgcctccag tggcaaaacc gtgaacgccg ccgacatcga
      961 tttgctggta cgcgccctga gcatgggcaa attgcaccac gcgatgatgg gtaccgcctc
     1021 tgttgccatt gcgaccgccg ccgccgtgcc cggtacgctg gtcaaccttg ccgcaggcgg
     1081 cggaacgcgt aaagaagtgc gcttcgggca tccttccggc acattgcgcg tcggtgcagc
     1141 cgccgaatgt caggacggac aatggacggc caccaaagcg gttatgagcc gcagcgcacg
     1201 cgtgatgatg gaaggttggg tcagggtgcc ggaagattgt ttttaaattg acgtagcatg
     1261 ggtttgcccg cgagccataa acaggtcgtc tgaaaaacaa gtaaacatca aatcactgac
     1321 cattcctttc ccttgccctg tggcggaagg cggcaaatca caaggaagaa cacggaaacc
     1381 ccgataaaag acagcttccc gtattaccgt cattcccgcg caggcgggaa tctagacctg
     1441 tcaatatgga ggattggtag gggaaaacag gtttcgtgag ttctacattc tggattcccg
     1501 ccacagcctg tcctcgcgta ggcggggacg gaataacgat agaaaatgcg gcatacgctt
     1561 tgcccaaaga ggccgtctga aacaccttgc gcctgatgtc tgcctttttc agacgacccc
     1621 acaccaaaaa acaaccacaa actacaagga gaaacatcat gtccgaccaa ctcatcctcg
     1681 ttctgaactg cggcagttca tcgctcaaag gtgccgttat cgaccgcaaa agcggcagcg
     1741 tcgtcctaag ctgcctcggc gaacgcctga ccacgcccga agccgtcatt acgttcagca
     1801 aagacggcaa caaacgccaa gttcccctga gcggccgcaa ctgccacgcc ggcacggtgg
     1861 gtatgctgtt gaacgaactg gaaaaacacg aactgcacga ccgcatcaaa gccatcggcc
     1921 accgcatcgc ccacggcggc gaaaaataca gcgagtctgt tttgatcgac caggccgtaa
     1981 tggacgaact caatgcctgc attccgcttg cgccgctgca caaccccgcc aacatcagcg
     2041 gcatccttgc cgcacaggaa catttccccg gtctgcccaa tgtcggcgtg atggatactt
     2101 cgttccacca aaccatgccg gagcgtgcct acacttatgc cgtgccgcgc gagttgcgta
     2161 aaaaatacgc tttccgccgc tacggtttcc acggcaccag tatgcgttac gttgcccctg
     2221 aagccgcacg catcttgggc aaacctttgg aagacatccg catgattatt gcccacttag
     2281 gcaacggcgc atccattacc gccatcaaaa acggcaaatc cgtcgatacc agtatgggtt
     2341 tcacgccgat cgaaggtttg gtaatgggta cacgttgcgg cgacatcgat ccgggcgtat
     2401 acagctatct gacttcccac gccgggatgg atgttgccca agtggatgaa atgctgaaca
     2461 aaaaatcagg tttgctcggt atttccgaac tttccaacga ctgccgcacc ctcgaaatcg
     2521 ccgccgacga aggccacgaa ggcgcgcgcc tcgccctcga agtcatgacc taccgcctcg
     2581 ccaaatacat cgcttcgatg gctgtgggct gcggcggcgt tgacgcactc gtgttcaccg
     2641 gcggtatcgg cgaaaactcg cgtaatatcc gtgccaaaac cgtttcctat cttgatttct
     2701 tgggtctgca catcgacacc aaagccaata tggaaaaacg ctacggcaat tcgggcatta
     2761 tcagcccgac cgattcttct ccggctgttt tggttgtccc gaccaatgaa gaactgatga
     2821 ttgcctgcga cactgccgaa cttgccggca tcttgtagcc aaaaaaggga cgagtccgca
     2881 aaaatgccgt ctgaaatttt cagacggcat tttgtttggc gtttgggcgt acaatacgga
     2941 aaacatgacg ataaggaaac aaaccatggc acagtttttc gccgttcatc ccgacaaccc
     3001 ccaagaacgc ctcatcaagc aggcggtcga aatcgtcaat aaaggcggcg tggtcgttta
     3061 tccgaccgat tcctgttatg ccttgggctg caaactcggc gataaggcgg cgatggaacg
     3121 catactctcc atccgcaaaa tcgatttgaa acaccacctg accctgatgt gcgcagattt
     3181 gagcgagttg ggcacatacg ccaaagtcga caacgtacag tttcgtcagc ttaaagccgc
     3241 cacacccggg ccttatactt ttattttaca ggcgacgaag gatgtgccgg cgcgcacgct
     3301 gcacccgaaa cgcaaaacca tcgggctgcg tattcccgat aatgccattg cacaagccct
     3361 gctgggggaa ttgggcgagc cgcttttaag ctgcaccctg atgctgcccg aagacggcga
     3421 accattgacc gatccttatg aaatccgcga gcgtttggaa cacgccgtcg atttggtgat
     3481 tgacggcggc tggtgcggaa ccgagccgac caccgtcgtc gatatgaccg acggcacgga
     3541 actggtgcgc cggggttgtg gcgatacggc ggtgttcggt ttgtagggaa accgatgccg
     3601 tctgaagcat cggctgttca gacggcattg cgcgccttgc cggcggcagt ccgaaatgcc
     3661 ggcgcgtatc gcgctcggtc ggaatatccg tttgaaacgg cattttgatg cattactgca
     3721 ccgcaatcgg aattctcggt tcgtagagca ggtcgtaggt cggcttgttg agcaggtctt
     3781 ggagcgtgaa accgtccaga tacgtgaaaa acgacttcat tgcaccgccg agtatgcctg
     3841 tcagacggca ggacggtgta atcaggcatt cgttgttcga acccatacat tcgaccagct
     3901 gcatcggttc gaggtggcgg acgaccgagc cgatgttgat gcggtcgggc ggtgtggcaa
     3961 gccgcagacc gccgcctttt ccgcgcacgc tgtggaggaa gccgcctttg accagcgcgg
     4021 taacgacctt catcagatgg cttttggaaa tgccgtaggt tacggcgatg gtactgatgt
     4081 tgaccagcgc atcgtcgttg atggcagtgt agataaggac gcgcagcccg tagtccgtat
     4141 gttgtgtcaa atacatgatt ttctcggtat ggattgttat tcttatcggt acggtttaag
     4201 gttcacggac aataccttaa tggttgaaac cctgtccgtc ggggcggtag aatgcagcct
     4261 gtctgcggcg gtatgccgtc tgaaacatcc gcgctaccgt ttgagaattt gttattgtaa
     4321 ctcaaaatca tgaaaccgtt gaaacgacat cccgccctta tcgggctttc gcgtgaccac
     4381 caccattcgc tttccctgtg cgtgcgtctg ttgcggacgc cggaagaaag gcatcgggac
     4441 gaactcgaac cgcatttttc cgaattggaa acccattttc gcgaagaaga aaccaagttt
     4501 gccccaattt ggcagaatgt cgcccccgaa ttgaaacaac gtttcgagaa agaccacgcc
     4561 cgactgcggc agatgatggc aagccccgaa tacggtaacg cggcgtggaa taccgctttt
     4621 gccacaaccc tgcgcgacca cgcgcgcttt gaagaacgcg agctgtttcc cgccgccgaa
     4681 ccgtttttgc cggcatgatt ccgttttgcg gcaaatatat taatgataaa caaggaacac
     4741 acatgaaatt taccaagcac cccgtctggg caatggcgtt ccgcccgttt tattcgctgg
     4801 cggctctgta cggcgcattg tccgtattgc tgtggggttt cggctacacg ggaacgcacg
     4861 agctgtccgg tttctattgg cacgcgcatg agatgatttg gggttatgcc ggactggtcg
     4921 tcgtcgcctt cctgctgacc gccgtcgcca cttggacggg gcagccgccc acgcggggcg
     4981 gcgttctggt cggcttgact atcttttggc tggctgcgcg gattgccgcc tttatcccgg
     5041 gttggggtgc gtcggcaagc ggcatactcg gtacgctgtt tttctggtac ggcgcggtgt
     5101 gcatggcttt gcccgttatc cgttcacaga atcaacgcaa ctatgttgcc gtgttcgcgc
     5161 tgttcgtctt gggcggcacg catgcggcgt tccacgtcca gctgcacaac ggcaacctag
     5221 gcggactctt gagcggatta cagtcgggct tggtgatggt gtcgggtttt atcggtctga
     5281 ttggtacgcg gattatttcg ttttttacgt ccaaacggtt gaatgtgccg cagattccca
     5341 gtccgaaatg ggtggcgcag gcttcgctgt ggctgcccat gctgaccgcc atgctgatgg
     5401 cgcacggcgt gatgccttgg ctgtcggcgg ctttcgcgtt tgcggcaggc gtgatcttta
     5461 ccgtgcaggt gtaccgctgg tggtataaac ccgtgttgaa agagccgatg ctgtggattc
     5521 tgtttgccgg ctatctgttt accggattgg ggctgattgc ggtcggcgcg tcttatttca
     5581 aacccgcttt cctcaatctg ggtgtgcatc tgatcggggt cggcggtatc ggcgtgctga
     5641 ctttgggcat gatggcgcgt accgcgctcg gtcatacggg caatccgatt tatccgccgc
     5701 ccaaagccgt tcccgttgcg ttttggctga tgatggcggc aaccgccgtc cgtatggttg
     5761 ccgtattttc ttccggcact gcctacacgc acagcatacg cacctcttcg gttttgtttg
     5821 cactcgcgct tttggtgtat gcgtggaagt atattccttg gctgattcgt ccgcgttcgg
     5881 acggcaggcc cggttgagac aaaccgccgc agatttcggg tctgggcggt ttgcttttca
     5941 gacggcaggg cggtcagttg ccgtccagcc agcggtcgcg tgtggttttg gcttcttcaa
     6001 aatagcggta cagggcttcg cggtcgtcgg tggtcaggat gtttgccaaa acgtccaact
     6061 gtttgcccaa gccttgaacc agttgcagca ggctgtcttt gttggcaagg cagatgtccg
     6121 cccacacggc gggatgaccg gaggcgatgc gggtgaagtc ccgaaagccc gtggcggcga
     6181 atttcagata ttcctgtccg tcgggatggt cgagaatctg gtggacatag gcgaaggcgg
     6241 tcaggtgggg catatgggag acggcggcga aaaccgcgtc gtggcgttgc gcgtccatcg
     6301 tataaatttc cgccccgacc gcgtgccaca ggttttctat caaggcaatg ccgtctgaat
     6361 gttcgccgcc gtgtggcgtg atgatgagtt ttctgtggcg gaacagcccg aactgcgcgg
     6421 cttgcgcacc gcttctgtcc gaaccggcaa tcgggtgggc ggcgatgcag cggtgcaggc
     6481 ggtcgggcag acagcggcgg aaggcttcga tgaccgaaga tttggtgctg ccgacatcgg
     6541 aaatccaagt gtgttccggc aaaacggggc gcagcgcggt caaaatggcg ggaacggtgg
     6601 cgacgggcgt ggcaatcagt accaagtctg caccaccgat gctgtccgcg tcgatggcaa
     6661 cggaagcccg gtcaatcacg ccgcgttcca atgcacgttc gaggttgtcg cggtcggtgt
     6721 cgataccggt aacggtgcgg acgagtccct gccttttgag gtcgagaatg aacgaaccgc
     6781 cgatcagccc tacaccgatg agggcaatat ggttcaaaat gggcatttgt gtaaacggtt
     6841 ttcgcaaagt accgtcatgg tagcctatcg gcggaatatg ccgcaaggtc ggcaggaaaa
     6901 aggagaagaa atggacaaaa tcagagttgc cgccgtgcag atggtgtcgg gcgtgtcgcc
     6961 ggaaaccaac gtcgccgcca tgaaacgcct ggtcgcacgg gcggcggagc agggtgcgga
     7021 ttgggtgctg ctgcccgaat attgggtgct gatgggcgca aacgataccg acaaactcgc
     7081 gcttgccgaa cctttgggcg gcggacgctt tcagacggca ttgagtgaaa cggcgaaaga
     7141 atgcggtgtg gtgctgttcg gcggaaccgt gccgctgcaa agccccgagg cgggcaaggt
     7201 catgaatact ttattggtgt acgggcgcga cggcaaacgg acggggctgt accacaaaat
     7261 gcacctcttc ggcttttccg gtttgggcga acgctatgcc gaagccgata ccatccgcgc
     7321 gggcggggat gtgccgcact tgtcggcaga aggcgtgtcg gtggcggcgg gcatttgtta
     7381 cgatgtccgc tttcccgaat ttttccgccg ccagctgccg tttgacgtat tgatgctgcc
     7441 cgctgcgttt acgcacacga cgggcaaggc gcactgggag ctgcttttgc gcgcgcgtgc
     7501 tgtcgaaaac caatgttatg ttgtggcggc ggcgcagggc gggctgcacg aaaacggcag
     7561 gcgcacgttc ggacacagta tgattgtcga tccgtggggc gacgtgttgg acgtattgcc
     7621 cgaaggcgaa ggcgttgtta cggcagacat cgatgccaac cgcctgaaca gcgtccgcaa
     7681 ccgcctgccc gccttgaaat accgggtttt ggatgccgtc tgaaggttca gacggcatcg
     7741 gtgccgggaa atcagaagcg gtagcgcatg cccaatgaga cttcgtgggt tttgaagcgg
     7801 gtgttttcca agcgtcccca gttgtggtag cggtatccgg tgtccaaggt cagcttgggc
     7861 gtgatgtcga aaccgacacc ggcgatgaca ccaagaccca agctgctggt gctgtggctt
     7921 tcgtgatagg caggtttgga gatcggacct aatataattt tgcctggctc tgtagcacct
     7981 gcccctgctt tactgaaagt agtcgtggtt tcttttctca ccgaatgaac ctgatgttta
     8041 acgtgtccgt aggcgacgcg cgcaccgata tagggtttga atttatcgaa tttatcgttg
     8101 agtttgaaat cgtaaatggc ggataagccg agagaagaag aggcgtggaa gctgccgttt
     8161 tcctgatttt ccgtcttcag ttcttgccag ttgccttggt ttcttagcac ctcttttgtg
     8221 tttacggaat atttattgtt gttccatttt ctgtaactgg cataatctgc cgctatcctc
     8281 cagtcgccga aatcgtagcc gaccgacacc cgggggtgga tggaatgcgc acggatgttt
     8341 ctgaaataat cgcttactgt gcttgtgttg tttgcaccgg ttgctttcgg ataatcgtgg
     8401 gtaatacgtt cggcggcata agctaaatcc gcctgcacat aatacgggct gcggctgccg
     8461 tcttcacttg ccgcctgcgc tgtggaagag aagagaagag aagagaagag aagagaagag
     8521 aagagaagag aagagaagag aaggtttttt gggggctgga ttcattttcg actccgtatt
     8581 cggttttaac tgattaaaaa gaaa
//
LOCUS       DAXHSJ010000084         8571 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000084 DAXHSJ010000000
VERSION     DAXHSJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8571
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.084"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(154..3006)
                     /gene="gcvP"
                     /locus_tag="ACFM8U_001317"
     CDS             complement(154..3006)
                     /gene="gcvP"
                     /locus_tag="ACFM8U_001317"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC6334553.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAKADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVSADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDAFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTVGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLGEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGEWAHPYSREEAVFPQ
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3037..3396)
                     /locus_tag="ACFM8U_001318"
     CDS             complement(3037..3396)
                     /locus_tag="ACFM8U_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334554.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3395..3574
                     /locus_tag="ACFM8U_001319"
     CDS             3395..3574
                     /locus_tag="ACFM8U_001319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC6334555.1"
                     /translation="MPSEPAGRLFQKTPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYINNLGADKPLFI"
     gene            complement(3567..4208)
                     /locus_tag="ACFM8U_001320"
     CDS             complement(3567..4208)
                     /locus_tag="ACFM8U_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC6334556.1"
                     /translation="MPSEKPMNRRIFLLGAGALLLTACGKKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEAYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4198..4749)
                     /locus_tag="ACFM8U_001321"
     CDS             complement(4198..4749)
                     /locus_tag="ACFM8U_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC6334557.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKRQAFQTAFEGFDIDTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDTHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRNTLKYHPKWLDAV"
     gene            complement(4753..5271)
                     /locus_tag="ACFM8U_001322"
     CDS             complement(4753..5271)
                     /locus_tag="ACFM8U_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC6334558.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGSETAVK
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5271..6932)
                     /gene="pqiB"
                     /locus_tag="ACFM8U_001323"
     CDS             complement(5271..6932)
                     /gene="pqiB"
                     /locus_tag="ACFM8U_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC6334559.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQAKVVDLLDKFDKLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLAGNPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(6925..8385)
                     /locus_tag="ACFM8U_001324"
     CDS             complement(6925..8385)
                     /locus_tag="ACFM8U_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HFC6334560.1"
                     /translation="MGSAVGGQPLEKIFLPDTKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPRLDRGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1914 a         2215 c         2440 g         2002 t
ORIGIN      
        1 tttgtttgtc cgtgtggaaa tgacatcaaa gggtgtgaat aggcttttca tctttgaaat
       61 ctgtttgcac gatcggatga ataaaaagta ggatgataaa aaagctgatg tatggatact
      121 ggtttaaccc atttgtcttc ggggtttcaa cggtcagtct tcataatttt ccatcggtgg
      181 gcagctgcac acgaggttgc ggtcgccgta cacgtcgtcc acgcggttga cgaagggcca
      241 aaacttgtgt tcgcggacga agggctgcgg gaagacggct tcttcgcggg aatacggatg
      301 cgcccattcg ccggttatat ctgcggcggt gtgcggcgcg ttgaccagtg ggttgtcgtc
      361 tttcggccat tctccgtcga tgactttctg cacttcgcgg cgaatggatt tcagggcggc
      421 gataaagcgg tcgagttcgg ctttgctttc gctctcggtc ggctcgatca tcagcgtacc
      481 ggcaacgggg aaggaaaccg tcggggcgtg gaagccgtag tccatcaggc gtttggcgat
      541 gtcggtttcg gtaatgccgc tttcggcttt gagcggacgc aaatcgacga tacattcgtg
      601 cgcgacgcgg ccgtttttgc ctgtatacag gatcggatag tcttcgccca gccgtttggc
      661 aacgtagttg gcgttgagca atgcccagcg tgtcgcctgt tccatgcctt gtttgcccat
      721 catggtcaga tacatccaag taatcggcag tatggacgca gaaccgaagg ccgcagcagc
      781 aacggcggtt tggtcggcac tggcgctgtg ggtgtcggtc aaagcgtgtc ccggtgcgaa
      841 cggagccaga tgggctttca agccaatcgg acccatgccc ggaccgccgc cgccgtgagg
      901 gatacagaag gttttgtgca ggttcatgtg caacacgtcc gcgccgactt cggcaggctg
      961 catgatgccg atttgggcgt tgaggttggc gccgtccata taaacctgtc cgccgttttc
     1021 atggatgatg cggcagatgt cgcggatgcc ttcttcgtac acgccgtggg tggacggata
     1081 ggtaatcatg atggcggaca aagcgtcgcg gtgttgctcg gctttggctt tcaaatcgtc
     1141 gatgttgacg ttgccgtgtt cgtcggtatc gacgacgacg actttcaaac cgagcatggc
     1201 ggcggtggcg gggttggtgc cgtgggcgga tttgggaatc aggcagatgt tgcgctgcgc
     1261 ttcgccttgc gcttcctgat agcggcggat ggcaagcata ccgctgtatt cgccctgcgc
     1321 gccggagttg ggctggaagg aaattgcgtc aaagccggtg atggctttca ggctgttttc
     1381 catatcggca agcaattcgc ggtagccgac ggtttgcgct tcgggagcgt aagggtggat
     1441 gtcggagaac tccgcccaag taatcggcag catttccgca gtcgcgttga gtttcatggt
     1501 gcagctaccg agcgaaatca tgctgcggtt catcgccaaa tcgcggtctt cgagtttttt
     1561 caggtagcgc agcatttcgt gttcggtgtg gtaacggttg aacacaggat gttgcagaat
     1621 gtcatcttga cgcagcaatt cggcgttcag acggcctttg acatcatcgg caaatgcgaa
     1681 cgcatccttg ccggtaaacg cgcggtacaa atcaaccaaa tcttcgcatg ccgatgtttc
     1741 gtggaatgca gccgcaactt gagtatcgtt aacgcggcgc aggttatagc ccgattccaa
     1801 agcagcggca aacacttggt ctgctttctc tttgttgccg aaatcgacgg taacggtatc
     1861 gaagaagacc ttatgaacca cattcaggcc gtctgaaacc agcgcatcgg caaaggcaga
     1921 agccagcgcg tgaatgcggt tggcgatgcg tttcacacct tcagggccgt ggtaaacggc
     1981 gtacataccc gccaagttcg ccagcaatgc ctgcgcggta caaatattgg atgtcgcttt
     2041 ttcgcggcgg atgtgttgct cgcgggtgga caacgccatg cgcaaggcag gtttgcccga
     2101 tgcgtctttg gatacgccga tgatgcggcc cggggcggaa cgtttgaact cgtctttaaa
     2161 cgcgaaataa gcagcatgcg gaccgccgaa gcccatcggt acgccgaagc gttgggtgtt
     2221 gcccaaagca atatccgcgc ccaattcggc aggcgatttc agcaaaacca agctcataat
     2281 gtcggcggaa acggccacga ttgtgccttt ggctttcaga cggccgataa cgtcctgcaa
     2341 gtcttgcaca tcgccgtctt tgccgacgta ttggaacagc gcgccgaagt attcgccttc
     2401 atccgctttg gcaaaatcgc cgaccaccag ctcgaagccg aaatacttgg cacgggtttt
     2461 catcacgtcc aaagtttgcg gatacacgcg ctcgtccaca aagaaacgct cggattttac
     2521 cttgcccacg cggtgcgcca tcgccatcgc ttcggcggcg gcggtcgctt cgtccagcaa
     2581 agacgcgccc gccacaggga aaccggtcaa atcgatacac acttgttgga agttcaacaa
     2641 agcttccaaa cgaccctggg caatttccgc ctgatacggc gtgtaggcgg tataccagcc
     2701 cggattttcc aatacgttgc gcaaaatcac attcggcacg cgggtcgggt aatagcctaa
     2761 accgatatag gacttgttga tcacgttttt cgacgcaatg cctttcaatt ttgccaacgc
     2821 gtccgcttcg gtcagggcat cgggcaaatc gagttcggac ggcatacgga tgctttgcgg
     2881 cacggtgttg ccgacaaaat cgtccatact tttctcgccg acagcggcaa gcaacgccgc
     2941 ttcgtcgccg aaactcaaat gccgcgcggc aaattcgttg gggttgaaca gttcggacag
     3001 tttcatttct actccttcgg gtttgtaggg ctgccatcag gcagaatttt tagacatcgg
     3061 atgattcggg caagggtagc acaaaagacg ggataatggc ggacaatgcg ccagaatgca
     3121 gcaggtcagg acaagcagcg ccacaagcgg cagcgataag ggaaagccgc cgcccaaaac
     3181 catgaaacct gcaggcacca gccaagccaa tggatacgcc ggaagcggaa aaacaatatt
     3241 gagcagaacc aaaatcacga caaacagcgg cagacccgcc atcaggttgg cacgcctgta
     3301 tgttgcgtac gggtcggatg cttcttcagg ttcgcaatat tccatgattt cagcctgtcc
     3361 gggctttatc cacagcaggc tcggacggtc ggtcatgccg tctgaaccgg caggccgtct
     3421 ttttcaaaaa acgccatacc gctgcaactt catgcggctg tatggcgtag gctttgcact
     3481 tcagacggca tatgcgtccc gaagcagttt ggtgtcccag tcggttcatg attatataaa
     3541 caacttgggt gcggataaac ctttgtttat ctaaacccct gttttctcaa aaattgattc
     3601 aaatcttcgg caaatttccg atagcctttg ccgttggcgt ggatttggtc ggatttcaga
     3661 ttattatcgc ccaaaatttc cgcccacgcg ccgccgaaca gcggaatgcc gtatgcctcg
     3721 gacaaatcct catacagcgg atggtcgctc aaatgcccga acaacgcgcc cagtgtgatg
     3781 tgcggcacgc cgacgaggac ggcggggatg ttttccttct gcacggtttc gatgattttc
     3841 gcgatattgg cgcgggtctg ctccttggga actttgcgca gaaagtcgtt gccgcctatg
     3901 ccgacaatca caagcttggg tttgcgtgcc aacagcgcgg gcaggcgcga cagggcttgc
     3961 gcggatgtat cgcccgatac gccgccgttg acaatattcc aacccgtcag tttttgcagt
     4021 tgcgcggggt aggattcgcc ggggtttgcg ccgtagccga aggtgagcga atcgcccaag
     4081 gcaagtacgg tgcttccttc gggaattttg gcgtgggttc gggcggattt tttgccgcag
     4141 gcggtaagaa gcaacgcgcc cgcgccgagg aggaagattc ttctgttcat tggtttttca
     4201 gacggcatcg agccatttcg ggtggtattt cagggtgtta cggtaaaccg gacagctttc
     4261 ggcgtgcgcg cctttgaggt agccggtact catcagaaat tcgccgacga tttcgccgcc
     4321 gacgaatttg aaatgttttt taaagagttt aacccattcg tctttgcttc gcggatggtg
     4381 cgtgtcgagc cagttcttga acgagccgta ttcttgttgc aacgcttgga tttgccgtgc
     4441 attgaaaatg gcggcatcga ttttcaggcg gttgcggaca atgcccgcgt cggcaagcag
     4501 gcgttcgcgg tcggtgtcgt cgaaggcggc aaccgtatcg atgtcgaaac cttcaaatgc
     4561 cgtctgaaac gcctgccgct tcttcagcat cagcgtccag tttaatcctg cctgattgat
     4621 ttccaacacc agccgctcaa acaattcatt gtcgtcctca atcggaaaac cgtattgcgt
     4681 gtcgtggtaa tgtttgttcg ggttatcggt gttttcggga agtgaggcgg caaattcgca
     4741 atagttcacg gtttactcga ccatctgttg cgccgcctgt ttcagtccct gttcgagtgc
     4801 ggcggtcatg gcggcgtagc cgtcaccctg ctgttcggtt tcgatatgga aaggtctgtt
     4861 cgtaccgtcg ggtaggacgg cgtagccgct gatgagggtt ttgcccgtgt agctgccttg
     4921 gaatgcgtcg atatagaccg tccatttttc ggtactgccg ctgcgtgagg caggaacaaa
     4981 gatgcgtgtg ctgtccaaac ggttgaatgc attgctcaac gccgcttcga gcatatcgtc
     5041 caaggtgtct gcccaaacgt ggttttgtgc ggtgttgatg cggtaggggt cggtttgata
     5101 gaccagtccg ccgcgtttga gcggctcggc aagacggact ttgacggcgg tttcgctgcc
     5161 ttgcgttgca ggacggatgt agcggctgtc gggcaacacg aaatattgtg tgctttgcac
     5221 agtaccgcag gcggcaagcg tcagggcggc ggcaatcggg aaaaggcgca ttatcggctt
     5281 cctttcggga tggggtcttt gctgctgctg ttgaaaatca gcgcgttggg tttttctttc
     5341 aaagtattaa ttacgggttg aacgtctttt aaagttttgt ccaaactttg cagcgtattt
     5401 tgtacgtcgc cgtagatagg cgattgcggc gatacgcctt gcagggttgt gcgcaactct
     5461 ttcagggttt ggttcagttc gttcggaatg ttttgcgtct gcggattgcc ggccagtttg
     5521 tcaatggagc ttagggcggc attggcagat ttgagtgcgg acttgagctc ggcaagcgaa
     5581 ccgttcaatt cggcaaccgt cttatccaaa ggcagtttgt cgaacttgtc cagcaaatcc
     5641 accactttcg cctgcaaatc gtccaaaccg ccgccttgtg tagcgatgac ggtatcgcct
     5701 gcataaacgg tatgcggtcg cagcttgggc gaagacgaag gctgatcgtt caactcaatc
     5761 attttgctgc cggtcagcag gttgttgctg gagatggtgg cggtcaggcc tttgtttaaa
     5821 gccgtctgaa attgttgttt ccaatgctct ttgctttgtt cgtcggcatt gatttccaaa
     5881 cgggaaggct caatgcggat gcgcacggga atccagccgt tttcaaacag gtgcaggctg
     5941 tcgttgcggt caaaataagg gacatcggaa accacgccga cattcagccc tttgtattca
     6001 acaggcgaac cgacggtcag tccgcgcacg gattgtttga aaaacgcggt gtagtacagc
     6061 gagcggtcgt caggcaggtt ggcgacttcg ctgcggctgt cgtaaagcgt gaagctgtct
     6121 tcgcttttga cgtttttact gtttttggtt ttcggcgaat caaatgaaat cgcgcccgac
     6181 agcagggcag gcagaggggc ggaattgagt ttgatgccgc tgcctgtggt ttcgatatta
     6241 atgccgcttt ccagccaaaa acggctggcg gaatgaatca gtttgtcgtt ggggctttgg
     6301 atgaagatgg tgtaatgcac gctttggtcg gacgggtcga aatgcgcgct ttcgatttgc
     6361 ccgaccataa agttttcata caaaacaggg ctgttgacgt tgaggatacg gtcgttttta
     6421 ccaatcaaat tcaagcgcag accgctttgc ccgatggcgg taacgggcgg aatgtcctgc
     6481 acttggaaca cgtcttttgc ctcgtcgctt ttgccgggtg taaaggcgat gtacgaaccc
     6541 gaaagcagcg tacccaaacc ggttacgccg ctttggtcga tacgcggctt gaccacccaa
     6601 aactgtgtgt cgctgcggat gaggccggat acgtccgcat tgagttgggc ggttacttcc
     6661 acgcctttct ggtcgtcgcg cagtttgatt cgggtaacgc gtccgacatc gatgctcaat
     6721 accttaatga ccgtattgtt gacctcaatg ccttccgcgc tgtccatcaa gagcgtaacc
     6781 acaggccccc tgttgcggat ttccttaatc cacagccagc caccggcaat cagcgcaatc
     6841 agcgggacca gccagacggc ggagagcaag gtgttgtttt tgcggacgcg tgcttgggcg
     6901 tgtccgtttg gaggagggct gttgtcagtc atgtttttcc gtttcgttga aagcaatgcc
     6961 gtctgaagcg cgtttgtccc aaagcaggcg cgggtcgaaa taataggcgg acagcatcgt
     7021 cagaatcacg accaggcaga aatagactgc cgcactgccc ggaatgacgc gcgcggcata
     7081 agtgtggaac gaacacatca aaataataat cacaaaaata tcaatcatcg accagcggcc
     7141 gaccgcttcg gtgatgcggt agaggtgcga caatttcttt gcacccgttg gcaaagcgaa
     7201 gcgggcggac gcaatcaaaa ccgacattgc cgcaatcttc agtaccggca ccaaaatact
     7261 cgcgctgaaa ataaccgccg caatcagcct gtcgccctcg tcccacatat aagcgatgcc
     7321 gttaaggatg gtattgacct ccgtggcggc aggattggac gaaatcataa tcggcaggat
     7381 attggcaggg aaatacaaaa taaccgccgc cgtcagaaac gccgacgaaa tactcagact
     7441 tttcggccgt cggcggtaca gttccgcgcc gcacacaccg cagggggatt cggcactgtc
     7501 gcggaaatac aggcagcggc tgcaacaggt tttgccttcc gatgccgtct gaaccgcatt
     7561 atcccccgtc agccgcccga tttgaaaata cacccaatgc tggggaacgg ataccgaagt
     7621 ccgaatcagc ataaccgaca gcgcgaacat cagataaaac gccgacccga agcgaacctc
     7681 tgccacagac gagagcttga tatacgccac cagcgtggaa acaaaaaaca catccaccat
     7741 catcgcctgc ctcaagcgca ccatcacacg cgttgccaaa cgcagcgcag gatacacccg
     7801 tttccgtatc agcgcggcat agacatacag gcacagcagc agaaacagaa ccggcgcgcc
     7861 gaaggtcagc acaaacatca cttcggccaa aaaaccataa tcctgaaaca ccatcaggcg
     7921 catcatctcg ggcagcgaaa ggacggatgc cgcacccggt atcccgacct cgatatacgt
     7981 cataccgtaa gcaaacgcca tcaaaatcag cgaagccgcc gcataagcgg gcggggcgga
     8041 aaaaggatgc ctgcccaccc tgaagagctt atagccgcag cgcgggcaga acgccgcctc
     8101 ccccctgtcc aaccgaggca catccgcgcg gcagccgcat tccgggcaat ctaccgtatg
     8161 cggcggcagg gcttcgtcgc ggcagttgcc gcgaaggctg tgggggatat tttcggcaaa
     8221 cggcttcatc gggaaagtgg caaatataag tggcggcatt ataaaggaaa aaaggcgata
     8281 cacccaatca ggatgcaaaa ccggcaggtt cggcagtatg aacaaaccct tggtgtccgg
     8341 caaaaatatt ttttcgagcg gctgtccgcc gactgccgaa cccatttgat aaaatcccga
     8401 ttctgtaaag aatagtaaaa tacctgctta aaaacatcac gcaaatgata ttttttccaa
     8461 aaatatgtga taataaattt cgtttatgat tctttattat ttttatagtg gattaacaaa
     8521 aatcaggaca aggcggcgaa gccgcagaca gtacaaatag tacggaaccg a
//
LOCUS       DAXHSJ010000085         8494 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000085 DAXHSJ010000000
VERSION     DAXHSJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.085"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(185..661)
                     /locus_tag="ACFM8U_001325"
     CDS             complement(185..661)
                     /locus_tag="ACFM8U_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC6334561.1"
                     /translation="MLAACQSGRAPKNAHTASADTPAGESCKVIRGDTAGSGKNIIYR
                     CSNGQAAINAAIADGVLESEIPVSFGGGGVPVANGRNLVTRQAANGVGKSDSEACERA
                     LINAARKFQQTAGKLGGRSITGFHSYFDKQPLQGGQYDCQTGSFHVRVVMRTNVVR"
     gene            complement(794..2149)
                     /gene="yegQ"
                     /locus_tag="ACFM8U_001326"
     CDS             complement(794..2149)
                     /gene="yegQ"
                     /locus_tag="ACFM8U_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC6334562.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPGGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            2474..3262
                     /locus_tag="ACFM8U_001327"
     CDS             2474..3262
                     /locus_tag="ACFM8U_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC6334563.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            complement(3308..3907)
                     /locus_tag="ACFM8U_001328"
     CDS             complement(3308..3907)
                     /locus_tag="ACFM8U_001328"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC6334564.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKLGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            4035..4652
                     /gene="gmk"
                     /locus_tag="ACFM8U_001329"
     CDS             4035..4652
                     /gene="gmk"
                     /locus_tag="ACFM8U_001329"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC6334565.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4711..4917
                     /gene="rpoZ"
                     /locus_tag="ACFM8U_001330"
     CDS             4711..4917
                     /gene="rpoZ"
                     /locus_tag="ACFM8U_001330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC6334566.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            5010..7166
                     /locus_tag="ACFM8U_001331"
     CDS             5010..7166
                     /locus_tag="ACFM8U_001331"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6334567.1"
                     /translation="MPVPQPTAPYDHLTAEARTLLFRTASYLKPEEQVELEKAVAYAF
                     HAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGVNIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(7233..8417)
                     /gene="coaBC"
                     /locus_tag="ACFM8U_001332"
     CDS             complement(7233..8417)
                     /gene="coaBC"
                     /locus_tag="ACFM8U_001332"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC6334568.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGSGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTELPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKRLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
BASE COUNT         2001 a         1999 c         2319 g         2175 t
ORIGIN      
        1 cttcgtcgcc ttgtcctgat ttttgttaat ccactataat atagtgttga tagacgtatt
       61 taacttaaat gtattcaata ggataggtct cttcctaacc gaaaaaataa ttgtcttttc
      121 ctttgcaaat aattgaaatc aacagattac taaaacacag cctaattttt tgattgccga
      181 tatgttaacg gacgacattg gtgcgcatga ccacgcggac gtggaacgaa cctgtctgac
      241 agtcgtattg tccgccttgc agcggctgct tgtcgaagta gctgtggaag ccggtaatac
      301 tgcgtccgcc gagtttgcct gcggtctgtt ggaatttgcg ggcggcattg attagggcgc
      361 gttcgcaggc ctctgagtcg gatttgccaa cgccgtttgc tgcttgccgt gtaaccagat
      421 tcctgccgtt tgcaaccggt acgccgccgc cgccgaagct gacgggaatc tcggattcca
      481 atacgccgtc tgcaatggct gcatttattg ccgcctgacc gttgctgcaa cggtaaatga
      541 tgtttttgcc gcttcctgcg gtgtcgcccc ggatgacttt gcatgattct ccggccggtg
      601 tgtcggcgga tgccgtgtgc gcgtttttag gtgcgcggcc ggattggcag gctgccagca
      661 tcagggcggc ggcggagagt atgaggattt ttttcatgtc ggatgtcctt gtttgctgtg
      721 ggaaatcatt gccattgtac cgtaagattc aacaaaatgc cgtctgaaaa cgggtttcag
      781 acggcatcgt ggcttagggg ttcaatacgc gtgcgatgag ggctttttct ttaccttgca
      841 tattggggat tttgacctga atgccgttgc cgggtgcgac atcgacaggc tggcctttgc
      901 gggtcatttg ttccaatttg atggtttggt tgccgcccgg gtggatgatt tcgagcgaat
      961 cgccgacggc gaagcggttt ttaacttcga tggtcgccca gccgttttcg tcgatttcgg
     1021 taacgtggcc gacatattgg ctttgtttgg cggtggaatg gccgctgagg tagttttgat
     1081 agtcttgggt ttggtggcgt tcgaggaagc cgctggtgta gccgcggttg gcaaggcctt
     1141 cgagttcgct caataggctg taatcaaacg gacggcccgc gacggcatcg tcaatcgctt
     1201 tgcggtagga ctgggcgacg cgtgcaacat aatagagcga cttggtgcgg ccttcgactt
     1261 tgaggctgtc cacgccgatt ttggcgagtt tctcaaccac ttcaatgccg cgcaggtctt
     1321 tggagttcat gatgtaagtg ccgtgttcgt cttccataat cggcatcatc tcgcccgggc
     1381 ggttggactc ttcaatcagg aaaactttgt cggcgtaggg gtggcgtttt tgaccgttga
     1441 tgccttcaaa gttttggttg gcttcttctt gggctttttc aaagttaaaa ccttgcagaa
     1501 gttgggcatc gcccgcatcg ctttcagtgg cgttgtggac tttgtaatcc cagcggcagg
     1561 agttggtgca ggtgccttgg ttggggtcgc ggtggttgaa atagcccgac aataggcaac
     1621 ggccggagta ggcgatgcac aatgcgccgt ggatgaagac ttcgagttcg atgtcggggc
     1681 attcttggcg gatttcggcg atttcttcca tgctcagttc gcgcgacagg atgatgcgtt
     1741 cgacgccgat gttctgccag aattttacac cccagtagtt ggtggtgttt gcctgtacgg
     1801 acaggtgaat cggcatttcc ggccattttt cgcgcacggt cataatcaaa cccggatccg
     1861 ccataatcag cgcgtcgggt ttcatcgcaa tcagcggctc catatcggcg acgaaggttt
     1921 tgagtttgga attgtgcggc agggtgttga cggttaaaaa gaattttttg ttgcgctcgt
     1981 gcgcttcttt aatgccttgc tcgagaacgt cgagtttggc aaattcgttg ttgcgggcac
     2041 gcagtgagta acgcgggctg ccggcgtaaa cggcatctgc gccgtagtca taggcggcgc
     2101 gcattctttc caatccgccg gcgggcaata agagttcggg tgctttcatc gtgtttcctt
     2161 ttcggttgaa accccgccct ttagggcggt agaatcagac tttatttggg agggacgtaa
     2221 ccctttccga atcagggcaa catatagggc gacgctttat gtgtcgtcct gtgtgttgaa
     2281 acatgatttg tatgctttgt aaaaatgttt cagacggcat tgctgcaaat gccgtctgaa
     2341 aagggtttga ggatgggcgg attatccgcc ttttcgtgtt cgcggtcaat tttgccggga
     2401 aatttataag gatttatcaa gtatttgcct gattgggata caatgcggca gtttgaatgc
     2461 gagagagaat gtgatgaatc ctaaaatcgt gtttttcgat attgacgata cgctgtaccg
     2521 caaatatacg gataccttgc gtccttccgt gaaaacggcg gtggcggctt tgcgcggcaa
     2581 aggcatattg acggcgttgg caacggggcg gtctttggcg acgattcccg aaaaggtcag
     2641 ggacatgatg gcggaaacgg gtatggatgc cgtggtaacg ataaacggac agtttgcgct
     2701 gctgcacggt aaaactgtgc gcgaagtacc gatggatgcc ggtttgatgg gcagggtttg
     2761 cgcgcatttg gatggcctgg gtatggatta tgcgtttgtc ggcggagagg ggattgccgt
     2821 gtccgcgctg tcggaatgcg tgtgccgcgc cttgaagcat atcgccagcg atttttttgc
     2881 cgataaggat tatttttcaa gcaaaccagt gtatcagatg ctggtgtttg cggaggaaaa
     2941 cgaaatgccg ccctggtcgg atattgtgga acgggaaggc ttgaaaacgg tgcgctggca
     3001 cgaggaagcg gtcgatctgc tgcctgcggg tgcgtcgaaa acggacggca tcagaagcgt
     3061 ggttgaagca ttgggtttgg aaatggcgga cgtgatggcg ttcggcgacg gtttgaacga
     3121 tgtggaaatg ctgtctgaag tcgggttcgg cgtggcaatg ggcaacgggg aacaggcggc
     3181 gaaagaagcg gcgaaatatg tttgccccgg cgttgatgag gatggtgtgt tgaggggttt
     3241 gcaagatttg ggcgtgattt gaacgcatca taacaaccct gccggtttca gacggcaggg
     3301 tcggttttca gcccttcata cagccttcgt tttgaagcag ggtaaataag ggcgcgccgc
     3361 ttgcacggat attcttgccg ccttgaaggt cggtaaattc caaaatggcg gcggcttcga
     3421 caatttcccc gccgagtttg cgaatcagct ctagtccggc aagcatcgtg ccgcccgtgg
     3481 caaccaaatc atcgaccagc agcacgcgcg aaccgagttt gacggcatcg gtgtggattt
     3541 ccaccgcagc ttccccgtat tcgagcgcgt agctttgcga tacggtttca aaaggcagct
     3601 tgcctttttt gcggatgggg acgaaaccga cgttgagctg gtaggcgagt gccgcgccga
     3661 taatgaagcc gcgcgcgtcc aaaccggcaa cgatgtcgat tttctgatcc atatagcggt
     3721 aaaccaataa atcaaccaaa aggcggaagt attccgcgct ttgcaggacg ggcgtgatgt
     3781 cgtggaataa gatgcctttt tgcggccagt tttcgatttt gcggattttg tcggcaagcg
     3841 caccgacact catagcttcg ggatgaacca gcattgcgtg ttccaagttt gatgttttta
     3901 acgccatatt gtagccgtca gcctgccgct tgccaaatca atcaaaacaa tagcggcaaa
     3961 atatgggcat cggcaaatag gaaaccgagt gtccgttgtg atacaatcgg ctggaaaata
     4021 tacaggtaaa aaatatgtcc gcttatcgaa aaggcaacat ctttatcatt tcggccgctt
     4081 ccggcacggg caaaaccacg ctggtgtcgc ggctgttggc aaaccataac ggtttgcgcg
     4141 tttccgtatc gcacacgacg cgtccgccgc gtgaaggcga agtaaacggc gtacattatc
     4201 attttgtttc taaagaagag tttgagtcgc ttatcgcgca ggaagctttt ttggaatacg
     4261 cagacgtgtt cggcaactat tacggcacaa gcgcggaggg cgtgaatgct ttggcggcgg
     4321 caggctatga cgtaattttg gaaatcgacg ttcagggcgc ggcgcaggtt cgcaacgcgc
     4381 tgccggaagc cgtcggcatc tttatcctgc cgccgtcttt cgatgtgctt gctgcacgtc
     4441 tgaacggacg cgggacggac agtcgggaag ttatccaaag gaggctgtcg aaggcaaggc
     4501 atgaaatcga acagtccgta ttgttcgatt ttgtcgtggt caatgatgac ttggcgcggg
     4561 cggaggagga tttgcgccat attgtgaatg cctgccgtct gaaaaggtcg cggcaactgg
     4621 gatttattgc agatttgttg gaaaattcct aaaaaaccgc gaaaataccc agtttcctat
     4681 tttaaatatt tttgaaagaa agcaaataat atggcacgta ttaccaccga agactgtacc
     4741 ggaaaaattt ccaaccattt tgacctgaca ttggtagcgg cccgtcgggc ccgccagctt
     4801 gaaaatggca atacgccgct tgtggacgat gtccgcaata acaaaccgac cgttaccgcc
     4861 ttaagggaaa tcgccgccgg gcatatcggt acggaactgt tgacgcgcaa taaataaatt
     4921 ctgccggaaa cgcacgtcgg cacattctgc cgccgtgcag tccgacggtt tgaaacgaaa
     4981 aataaaatat gttccgaaag ccgtctgaaa tgcctgtccc tcaacccacc gcaccttacg
     5041 accatttaac tgccgaagca cgcaccttgc ttttccgtac cgcttcctat ctcaagcctg
     5101 aagaacaggt tgagctcgag aaggccgttg cctatgcatt tcacgctcat gacggacaaa
     5161 cccggaaaag tggcgaacca tacattacgc atccaattgc cgttgcgacg cagctcgccc
     5221 tttggcatat ggacatacag ggtctgtgtg caggcgtgat gcacgacgta ttggaagata
     5281 cgggcgtgac aaaaggggaa atggcggcgg tgttcggcaa tacgattgcc gagatggtgg
     5341 acggtctgtc caaactcgaa aaactcaaat ttgaagatca tgcggaacat caggcggaga
     5401 gtttccgcaa actgattttg gcaatgacca aagatgtgcg cgtgattgtc gttaaacttg
     5461 ccgaccgtct gcataatatg cggacactcg gttcgatgcg cccggacaaa cgccgccgga
     5521 ttgcaaggga aacccttgaa atctatgcac agattgccaa ccgtataggt ttgaataacg
     5581 catatcaaga gcttcaggat ttatcgttcc aaaacctgca tcccaaccgc tacgagactt
     5641 taaaaaaagc gatggacaag agccggaaga accggcagga cgttgtcggc aaagtgttgc
     5701 gcgcattcgg ccagcggctg gtgggtgtga atatagaggc caaaatcaaa ggcagggaaa
     5761 aaaacctgta cggcatccat cagaaaatga tggcgaaaaa gctgcgcttt gccgaggtta
     5821 tggatattta cggtttccgc gtcattgtca acagcattcc agcctgttat gccgcactcg
     5881 gcgcattgca caccctctat cagcccaagc ccgggcggtt caaagactat atcgccattc
     5941 cgaaaagcaa cgggtatcaa agtctgcata cgactttggt cggtccttac ggcttgccga
     6001 ttgaagttca gatacgtacc aaggaaatgg atgctgttgc cgaaggtgga atcgccggac
     6061 attggagcta taaatcatat tctaagacgg tcgatcaggc ggtgcttcac acaaaccggt
     6121 ggctgaaaaa tatcttagat ttgcaggcaa gcagtgccaa tgccattgag tttctcgaac
     6181 acgtcaaagt cgatttgttt ccgaacgaaa tctacatcct tacgccaaaa ggaaaaatcc
     6241 taactttgcc caaaggggca acgcctgtcg attttgctta tgcggtgcat accgatatcg
     6301 ggcacaaaac cgttgccgca cgtatcaaca atatcatgat gccgttgcgt acgaagctca
     6361 aaaccggtga ttctgttgaa attatcacat ccgaacacgc caaacccaat cccgcgtggt
     6421 tgaatttcgc cgtgtcaggc agggcgcgca gtgccatacg ccaatatatt aaaaacctta
     6481 accggcacga tgcggtcgtt ttgggagaga gtctcttaca aaaagccctg tccagtttgc
     6541 tgcccaaaga tgtcctgctt tcagacggca tcaaggaaaa atatcttgcc gatctcaacg
     6601 acaaacagac ttcatttgag gaagtgctgt acaacgtcgg tatggggcat accctgcctg
     6661 tttatgtcgc catgcacatt gctgagttgg caggggagca tttcggcagc gaggtcaggc
     6721 tcagttccat taaagtcgat ggtcaggaaa gcgggcatat tcattttgca gagtgctgcc
     6781 accctgttcc cggcgattcc atccgtttgc tgttggttaa gggaaaaggc atgattatcc
     6841 atagggatag ctgcccgacg ttgttgaagt ccgatcccga acagcagctg gatgcagact
     6901 gggaaaatat gaatgggcag aactaccgtg tcgggcttca agtccaatcg gaagacagcc
     6961 acggcctgtt ggcattaatg gcgcaagcga tttccgattc cggtgcggac atcgagtcgg
     7021 tcgaaacacc atccaaatcc cagtcgggaa cggaaggttt tgtcgaattc aaattcttat
     7081 tgaaagtcaa gaatctgaat cagttgaatc agattattca aaatctgcat agtattccat
     7141 atatccgcaa agtcatcaga agttgaagca ggtaaaacgg gttgcctttt gattaacagg
     7201 caacccgttt tatggtttat ccgttcaatt gcttatttgt tcaaatatac ggcaagcctt
     7261 tcaacaatct gcatcgccgc ttcatcttta cttgtttcgg gaaaagatag ttccccgtcg
     7321 tccccgataa tggtaatccg gttggtcggt ttgcccatcg cgattgaaac atcattggca
     7381 actattaccg gtattttttt cttaatacgt ttttctcgtg catatgccat tacattctcc
     7441 gtttcagcgg caaaaccgat gcagaacggc gggttcggca atgaggcaat agaagccaaa
     7501 atatcagggt tctcatccaa ttcgatggat agccgtttgg catttttatc ttttttgaat
     7561 ttttgcgtac tcctgttttt aaccttatag tctgagacgg cggcaacaga aataaaagca
     7621 tcttgttttt caatcaaacg atgcactgcg cgatgcatat cttcagcgct gacggcttga
     7681 accgtatcgg atatgccgaa aggcagttcg gtttgaagct gtccgtgaat caggctgact
     7741 tccgcaccgg cggcacggca cgcccgcgcc aaagctaccc ccatttttcc gctggaggta
     7801 tttgtgatgc ccctgacagg gtcaatggct tcaaatgtcg cacctgcggt aatcaagatt
     7861 ttcttgcccc ttaaaatttt cggtgtccac aagtccggaa gcaaatccag caattcggca
     7921 ggttccggca tccttcccat accattttct ccgcaagcct gttcgcccga gcccggcata
     7981 tagacagtaa tgccgtctga aaccagttgt gcgatattcc gttggttggc agggttgagc
     8041 cacatttcca cattcatcgc gggagcgatg gcgagcggac atttccgtgc ggctgccaga
     8101 ctggtcagta ggttatctgc cacgccgtta cagatttttg ccacggtatt catacttgcc
     8161 ggcgcaatca gaaaaacatc cgcattccgg gtcaggttga tatgttccat accgtttgaa
     8221 ccgttgccgc cgtgcgtgtc ggtcaggaca ggattgccgc ttaaagcctg aaaagtcagc
     8281 ggagaaacaa attcagttgc cgagcggctc ataaccaccg taaccgaatg cccctgtttt
     8341 ttcagcagac gaaccaactc gcaagactta tacgccgcaa tactgcccgt tacacctaaa
     8401 agaatatgtt tacccatttt gacaatttct aattacgaag aaaaaacagc agccattcta
     8461 taacaaagtg cggatacatt agttaccgtt tgga
//
LOCUS       DAXHSJ010000086         8249 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000086 DAXHSJ010000000
VERSION     DAXHSJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.086"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(147..1079)
                     /gene="cysK"
                     /locus_tag="ACFM8U_001333"
     CDS             complement(147..1079)
                     /gene="cysK"
                     /locus_tag="ACFM8U_001333"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC6334569.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVPNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1273..1878)
                     /locus_tag="ACFM8U_001334"
     CDS             complement(1273..1878)
                     /locus_tag="ACFM8U_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334570.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1875..2726)
                     /gene="dapF"
                     /locus_tag="ACFM8U_001335"
     CDS             complement(1875..2726)
                     /gene="dapF"
                     /locus_tag="ACFM8U_001335"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC6334571.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            2863..3159
                     /locus_tag="ACFM8U_001336"
     CDS             2863..3159
                     /locus_tag="ACFM8U_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334572.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADSAVKGGFYKIIEIQGANRILIQDFNSTGNALGGFYVPDCRKSSLSPKNI"
     gene            3173..3841
                     /locus_tag="ACFM8U_001337"
     CDS             3173..3841
                     /locus_tag="ACFM8U_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6334573.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3914..6034)
                     /gene="pnp"
                     /locus_tag="ACFM8U_001338"
     CDS             complement(3914..6034)
                     /gene="pnp"
                     /locus_tag="ACFM8U_001338"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC6334574.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVREIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RVGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6271..7134)
                     /locus_tag="ACFM8U_001339"
     CDS             complement(6271..7134)
                     /locus_tag="ACFM8U_001339"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC6334575.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKVLEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERSIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDENGRLTLMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7334..8197
                     /gene="rfbD"
                     /locus_tag="ACFM8U_001340"
     CDS             7334..8197
                     /gene="rfbD"
                     /locus_tag="ACFM8U_001340"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC6334576.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSATIIRLLQHSNPVRGIYHYAGGKSVSWYE
                     FVQHIFQAASQQQTSFPIPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         1986 a         2374 c         1899 g         1990 t
ORIGIN      
        1 atttccctca agcgatgcgg cgacaccaac acctgattaa aacacaaccg gcgttttcgg
       61 catatatgca aaaacgcatt tcccagcacg gcgttacacc gataccgaaa aatgcgtctg
      121 aatgtttcga caatccgatt aaagcattat gccaaatctg caaaaagtgg cgtagagaga
      181 tagcgttcgc cataagaagg cagcagcacg actatcagct tgccttcgtt ttcaggctgt
      241 ttggcaagct gcaacgcact ccaaaccgcc gcaccggaag aaatacccac caaaatgcct
      301 tctttttccg ctattgcgcg ggcggtttca aaagccgctt cgttcggcac tttggtaatg
      361 ctgtcgtaaa ttttggtatt caaaacggtc ggaataaagc ctgcgccgat gccttgaatc
      421 gggtgcgggc ctttttcgcc gccgctcaat acgggtgaag cttcaggctc gacggcaacc
      481 actttaactt cgggtttgta ttttttcaac acttcgccca cgccggtaat cgtaccgccc
      541 gtgccgacgc cggcaacgaa gacatcgact ttgccgtccg tatcccgcca aatttcctcg
      601 gcggttgttt tgcggtggac ttcggggttt gcctcattgt cgaactggcg cggcataaaa
      661 taagtgtcgg gatgcgcgtc caccaaggat ttcgctttgg caatcgcgcc cgccatacct
      721 tcggcggcag gggtcagaat cagctccgca ccaaacgcgc gcaacagcat tttgcgctcc
      781 ttactcatgc tttccggcat ggtaatcgcc agcttgtagc cgcgtgcggc acataccatt
      841 gccaaaccga cacccgtatt gccgctggtt gcttcgacaa tgacggtgtt tttgttgatt
      901 ttgcccgctt tttcggcacc ctcgatcatt gcttcggcaa tgcggtcttt gacgctgctg
      961 cctggattga aaaattccag tttcacggca acctccgcct tcaaaccttc ggtcagacgg
     1021 ttcagtttga ccaaaggcgt gttgccgatt agttcggtga tgctgtttgc aattttcatc
     1081 ttctgctcct gaataataag gtttcgacag taaggcgcaa tccccatagg catcctgtcc
     1141 gattgcaggg gatttgcgcc gaaacataaa caaatttcgg atatggcttt atcctaaacc
     1201 tatgccgcct aaaaaaccaa tatccaaaat ttctttcttt ataacttttt gttccgatac
     1261 ggccgcaccg tattatcttc caatttctga aagcagcagc accagtaccg agacctgcat
     1321 caacgctgcc gtcaacccgc cgagtcgcag ctgcgagccg ccgaacgttg acggcggctg
     1381 ccggtctata ccgcagcggt ggacataggc aaccgccatc agcacaaact gcaacacgaa
     1441 ccaataaacc ggcttcagcg cataaagttt gagcagtgcc gcaaacacaa acacttgaac
     1501 cgttcccgaa aagcaataat gcaccaacgc acatattccg acaaaagcaa gcgtcatcga
     1561 tacattactg acggcaaaca gcccgagcag aggatgttcg gggtctgcct gccatccgtt
     1621 tccatccgtt ttccggttcc aatgcccgat gaaaaaaaat atgctgccca aagtcaggta
     1681 aaaatggggg atgaacaagg gcgcggcgcg ggtcattccc catatgccgg gcatcagctt
     1741 tgcccccaaa accgatatgc ccagccacag cataatactc gcccaaaacc attgcaacat
     1801 tcctgcgcgg aatatcagaa acagaaaaac cgccgcatac agtaaggcag acaaaaaatc
     1861 caaatgcagc aaaatcatga atacgccaac tcgccttcaa acaccgcttc cgcagggccg
     1921 gtcatcatca catcgccgcc gcaggcccat tcgatatata aagtcccgcc cggcaaaatc
     1981 acctctaccg ttttcccttc atccaacaac cccagacgga tacccgccac cacagccgca
     2041 cacgcgcccg taccgcacgc ttgggtttcg cccacgccgc gctcgaacac gcgcaaacga
     2101 atcgcggttc ggctgacaat ctgcataaag ccgacgttga cgcgttcggg aaactgcctg
     2161 tgcggttcga taagcgaacc ggtttcgcgc accggcgcgc attctacgtc atcgaccaca
     2221 atcaccgcat gggggttgcc catattgacg cagctgacag gctgaatgcc ggattcgaga
     2281 tgcaccccgt aaatacaggc atcatccccc tcgcccgatt cggggacaaa cggtatttca
     2341 gacggcataa acttcggttt gcccatattg accgtaacca tgccgttatc ggacaatttc
     2401 ggaaaaataa cgccatttgc cgtttcaaca caaatttctt tcttatcggt caaacccttg
     2461 tctgcaacaa aacgggcaaa acaacgcgct ccgttgccgc attgcccgac ctcgctgccg
     2521 tcggcattga aaatacggta acggaaatcc acgccttcgg tttccgaacg cccgaccacc
     2581 aaaagctggt cgaagcccac gcctcggaag cggtccgccc atgccgcaat cggcgcgtcc
     2641 tcgggggtaa aatcctgact gaccgcgtca atcaccataa aatcgttgcc caaaccgtgc
     2701 attttagtaa atttcaaagt gttcatatta ttctgaaatc ccgtattgtc tgttgcttcc
     2761 gaattccgaa atccccgtgc cgaagggaaa tgcttccatc tcccgccgcc tgaggattaa
     2821 aatcagccat tatactgttt gttacccctt acaagaaaca ccatgttccg catattactg
     2881 ttgcttttcc cttgtgcaat ccttgccgcc tgcacccgaa acataccggc aaacccggcc
     2941 gtttcaaatt gcgcgcatct tcaaaaccgc accatcctgt atttcgacgc acacggcaaa
     3001 cccgccgaca gcgcagtaaa gggcggattt tacaaaatta tcgaaataca gggtgcaaac
     3061 cgcatcctta ttcaagactt taacagcacg ggcaatgccc tcggcggatt ctatgtgccg
     3121 gactgccgga aatcatccct ttcaccgaaa aatatctgac ttcaaactgc ctatgaacca
     3181 cgacatcact ttcctcaccc tgttcctact cggtttcttc ggcggaacgc actgcatcgg
     3241 tatgtgcggc ggattaagca gcgcgtttgc gctccaactc cccccgcata tcaaccgctt
     3301 ttggctgatc ctgctgctta acacaggacg ggtaagcagc tatacggcaa tcggcctgat
     3361 actcggatta atcggacagg tcggcgtttc actcgaccaa acccgcgtcc tgcagaatat
     3421 tttatacacg gccgccaacc tcctgctgct ctttttaggc ttatacttga gcggtatttc
     3481 ttccttggcg gcaaaaatcg agaaaatcgg caaaccgata tggcggaacc tgaacccgat
     3541 actcaaccgg ctgttaccca taaaatccat acccgcctgc cttgcggtcg gaatattatg
     3601 gggctggctg ccgtgcggac tggtttacag cgcgtcgctt tacgcgctgg gaagcggtag
     3661 tgcggcaacg ggcgggttat atatgcttgc ctttgcactg ggtacgctgc ccaatctttt
     3721 agcaatcggc attttttccc tgcaactgaa aaaaatcatg caaaaccgat atatccgcct
     3781 gtgtacggga ttatccgtat cattatgggc attatggaaa cttgccgtcc tgtggctgta
     3841 agccccattc attcccgata cccgtaaaaa ataccgtctg aaacctgttc agacggcact
     3901 ttcaccctaa gcgttactcg gcggcatttt cctcacgggc aggcgcgtcc agcagggctt
     3961 tgatggacag acggacacgg cctctgtcgt ccacttccaa tgctttcacg ttcaccacct
     4021 gaccgacttg caggtagtcg ccgacattgc gtacgcgctc gtgggcgatt tggctgatgt
     4081 gtaccaaacc gtctttgccc ggcatcacac tgacaatcgc gccgacattg ttgtcgagga
     4141 ttttcaccac agtgccttcg tacactttgc ccacttccac ttcggcagta atctgctcga
     4201 tgcgtttttt cgccgcatcg ccggcttctt gagtggttgc ggcaatggta atcgtaccgt
     4261 cttcggcaat attgatttcc gtaccggttt cagcggtaat cgcacggatg gtttcaccgc
     4321 ccttaccgat aacttcgcgg attttgtctt ggttgatttt catcgtgaac aagcgtggcg
     4381 cgtgtgcgga cagctcttgc gggcccgcaa cggcggcttt catctgatcc aagatgtgca
     4441 gacgcgcttc tttggcctgt gccaaagcga tttgcatgat ttctttggta ataccttgga
     4501 ttttgatgtc catttgcagc gcggttacac cttcggtcgt accggctact ttaaagtcca
     4561 tatcgcccaa gtggtcttcg tcgcccaaga tgtcggtcag gacggcaaat ttgttgcctt
     4621 ccagaatcag acccatagcg ataccggcaa cgtgtgcttt caaaggcacg ccggcggaca
     4681 gcaggctcag gcagccgccg cagacggaag ccatagagga agagccgttg gattcggtaa
     4741 tttcggagac cacgcgcatg gtgtagctga aatcttcagg tttcggcaat acggccaaca
     4801 atgcacgttt agccaaacgg ccgtgaccga tttcacgacg ttttggcgca cctacgcgac
     4861 ccacttcgcc ggtagagtat ggaggaaagt tgtagtgcag catgaagcgg tcggtgtatt
     4921 caccggacag cgcatcaatg atttgctcgt cgcgtgaagt acccaaagtt gcaaccgcca
     4981 aagcttgagt ttcgccacgg gtaaacaatg cagaaccgtg cgtgcgcggc aatacgccgg
     5041 tttgaatgtt cagcggacga acagtgcggg tgtcgcgacc atcaatacgc ggttggccgt
     5101 ctaaaatttg gctgcggaca acatcggctt ccaagtgttt gaaaatgcct ttgatttcgt
     5161 tggctgccaa agtgtcggtt tcctcggtaa tcaaggcttc ttttacagca ctccaagctt
     5221 cgtccaattt ggcagaacgc gcttgttttt gacggatttt gaatgcttct ttaatggttt
     5281 caccggcaat ttcgcgtact tttgcaacca attcttcatt ggtttcaggt gctttccaat
     5341 cccaaagttc cggattgact tcgtcggcaa attcattgat tgcattgatg gcaacctgca
     5401 tttgatcgtg gccgtaaacc accgcgccca gcatcacgtc ttcgggcagg attttggctt
     5461 cggattccac catcaacacg gcttttgaag taccggcgac caccaagtcc aattgcgatt
     5521 tcgccaattc ggctttagtc gggttcaaaa cgtacacgcc gtttacataa ccgacgcgtg
     5581 ccgcgccgat cgggccggca aacggtacgc cgctcaacac cagcgcggca gatgcaccca
     5641 acattgcagg aatatcagaa tcgatttcag gatcgacgga cacgaccatc gctacgattt
     5701 ggatgtcgtg gtagaaacct tcagggaaca gcggacgaat cggacggtcg atcagacggc
     5761 tggtcaggat ttctttttcg ctttgtttgc cttcgcgttt gaagaaaccg ccgggaattt
     5821 tgcctgcggc gtaagtgcgc tcgaggtaat cgacagtcag agggaagaag tcttggcctt
     5881 ctttcacttc tttgttggtg gtaacggcaa ccaaaaccac ggtgtcgccc atagagactt
     5941 taacggcagc ggcggcttgg cgggcaattt cgccggtttc caaagtaacg gtgtggttgc
     6001 cgtattggaa agttttaacg tgtttgtcga acattattgt tcctttcaaa ataccgcact
     6061 gctaaaacac taataatgca cactaaaatc cgaatgtgca tagttagggt ttcagaccgt
     6121 gcggcaggtt gtaaacaagc tttcagacga catttcggac gctgaaagca attgcggatt
     6181 ataaaggcaa ccatccttaa aatccagtat tgatacaata aaaaggtcgt ctgaaccata
     6241 tttctttcag acggccttca aacttaaaaa tcaatcctga gtcagcaaag tcaatgcttc
     6301 ccgatacttc tcgacagttt tctgaatcac atcggcaggc actttcggcg caggggcttt
     6361 tttgttccaa ccgctttgtt ccagccagtc gcggacgaat tgtttgtcaa aagacggcgg
     6421 attggtgccg acttcatatt gatccgccgg ccagaagcgg ctcgaatcgg gagtcaatac
     6481 ctcatccatc agcgtcagcc taccgttctc atccaaaccg aattcaaatt tggtatcgca
     6541 aatgatgatg ccgcgcgatt tagcatattc cgccgcttct gtataaagct gaatcgcttt
     6601 ggcgcgcact tccgccgcca attcttttcc gatactgcgt tcgcattctt caaagctgat
     6661 gttttcatcg tgatcgccga ctgcggcttt ggttgagggc gtaaaaatca cttcaggcag
     6721 ttgttgcgct tcctgcatac cttcgggcag ccgaataccg caaaccgagc cggttttttg
     6781 ataatctttc caaccgctgc ctgccagata accgcgcaca atcgcctcta ctttcaccgg
     6841 agtgagcttt ttagccacga cggcgcgttt ttctaaaact ttggcttcgt tttcaggcaa
     6901 aacatcgtaa accgtttgac cggtaaagtg gttgggcata atatgcgcca gttttttaaa
     6961 ccaaaaattg gaaatctgcg tcagaatctc ccctttgctc ggaatcgggt cgtccaaaat
     7021 cacatcaaac gcggacaggc ggtcggaagc gaccatcagc atacgtttat cgtcgatttc
     7081 atataaatcg cgcacttttc cagaatagat ctttaccaaa ccaatctcac tcatttcgcc
     7141 cccccctgaa aatatcttga aaataccgac ccgacacccg acaggtttga atcgcaaacc
     7201 gatattctag ccgaagtcgg cgcaaaacaa tacccatggc acaaaaagcc aacccgtcaa
     7261 ccgtcggcaa agttttggca ctataatacc gacagcaagt cctacaatac acttttacca
     7321 aaggaaatac ctcatgagaa tcctattgac aggctcgaaa agccaactgg cacgctgcct
     7381 gcgcgaccgt cttccggaag actgggaaac cattgcgacg gattccgcat ccctagacat
     7441 taccgatgcc gatgccgtct gcaacatggt caaaagtttc caacccgacg ccattgtcaa
     7501 cacggctgcc tatactgccg tcgacaaagc ggaaggcgat gcggcagcgg catttgccgt
     7561 caatgcttcc gccgtttaca accttgcctt ggcagcacat cgcgcccatg cccgattcat
     7621 ccacatctca accgactatg tatttgacgg taaagggaaa agaccctatc aggaaagcga
     7681 ctttaccaat ccttccaatg tatacggaca atccaaaacc gcaggcgagc tgctcgcact
     7741 gtctgccaat cccgacagcc ttatcctgcg gacttcttgg ctgtttagcg aatacgggga
     7801 caactttgtc cgcacgatgc tgaaccttgc gcgggaacgt tccccgctgt ccgccgtcca
     7861 caaccaaatc ggctgcccga cctatgccgg cgacttgtcc gccaccatca tccgcctgtt
     7921 gcagcactcc aatcccgttc gcggcattta ccactacgcc ggcggcaaat ccgtatcttg
     7981 gtacgaattt gtccaacata ttttccaagc ggcatcgcaa cagcagacat ccttccccat
     8041 tcccgaattg actgccgttt cagacaagga atatccgacc gccgccccca ggcccgcata
     8101 cagcattttg gactgccgca aaatcgaaaa cgacttcggc atcaaaccgt cagactggca
     8161 aaaagccctt gcgcaggtcg tttccaagct gctctgatgc cgcccgcctc tccgtttccg
     8221 ccgtcaagca ccgccttggc ggttttctt
//
LOCUS       DAXHSJ010000087         8243 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000087 DAXHSJ010000000
VERSION     DAXHSJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.087"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(60..1505)
                     /gene="zwf"
                     /locus_tag="ACFM8U_001341"
     CDS             complement(60..1505)
                     /gene="zwf"
                     /locus_tag="ACFM8U_001341"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC6334577.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            2022..3857
                     /gene="edd"
                     /locus_tag="ACFM8U_001342"
     CDS             2022..3857
                     /gene="edd"
                     /locus_tag="ACFM8U_001342"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC6334578.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            4038..4676
                     /locus_tag="ACFM8U_001343"
     CDS             4038..4676
                     /locus_tag="ACFM8U_001343"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC6334579.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            4691..4909
                     /locus_tag="ACFM8U_001344"
     CDS             4691..4909
                     /locus_tag="ACFM8U_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334580.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(4933..5974)
                     /locus_tag="ACFM8U_001345"
                     /pseudo
     CDS             complement(4933..5974)
                     /locus_tag="ACFM8U_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            6137..7177
                     /gene="mutY"
                     /locus_tag="ACFM8U_001346"
     CDS             6137..7177
                     /gene="mutY"
                     /locus_tag="ACFM8U_001346"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC6334581.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     SELPRKKTAAEVPTLPLYWLIIRNRNGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            7594..8154
                     /gene="sodC"
                     /locus_tag="ACFM8U_001347"
     CDS             7594..8154
                     /gene="sodC"
                     /locus_tag="ACFM8U_001347"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFC6334582.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         2073 a         2277 c         1970 g         1923 t
ORIGIN      
        1 tgctttactt gcgttcagac ggcatttcaa accccatgcc gtctgaacgc attattgtat
       61 tactgctctt cgtgccactt gtgtccgtcg cgcgccaata gttcgcgcgc ggcttcaggc
      121 ccccacgagt gtgcgccgta gccgtgcggc ggcgtggtgt tatttgtcca gttttccaaa
      181 atcggcatca catattccca cgcggcttca agttcgtcgc ggcggttgaa caaagcgagt
      241 ttgccgttaa tcacatccag cagcaggcgc tcgtaagctt ccgcgcggcg gctttccaat
      301 gctttgccca aatcggttgc cagcggcacg gtttcgacct tatttcctgc ccccggggtt
      361 ttcatctgcg tatagaggcg cacggattca tatggttgca actcgataac gagccggttg
      421 ggcgcggtgc ggctgccttc aaaaatatgg ctgttcaaat ctttgaagtt caaaacgatt
      481 tccgccactt tgcccgccat gcgcttgccg gtacgcaggt agaagggaac gcccttccag
      541 cgttcgtttt cgatttcggc tttaatggcg acgtaggttt cggtaaagct gtcttgcgga
      601 acgttgattt cttcaagata gccgttcatg cctttggcgg cggtatattg tccgcgcacg
      661 acattttcat tgacagattc gacggtcagc ggcttcaatg acttgatgac tttgactttt
      721 tcatcgcgca ccgcgtcggc atccaagctg gcgggggctt ccatcgcagt catgcacagc
      781 atttgcatca agtggttttg caccatatcg cgcaaggcgc cagtaatgtc gtaaaactcg
      841 ccgcgctctt ccacaccgag ctgttcggcg atggtcaact gcacgctttc gatgtattta
      901 ttgttccaca gcggctcgaa cattacattg gcaaaacgca gcgcaagcag gttttgcagg
      961 ctttctttgc caaggtagtg gtcgatgcgg taaatttgcc cttctttgaa ataacgcgca
     1021 acatcggtat tgatttgctg ggaagaagcc aaatccgtac ccaacggttt ttccaaaacc
     1081 acacgcacgt tgtcggcgtt caaaccgatt ttggcaaggt tttcacatgt ttgcgcgaag
     1141 aatttcggcg cggtggaaag atagatgacg acattgtcgg tttctttgcg cgctttgacc
     1201 aaatcgccca aagcggcaaa atcgtccggc tgcgtaacat cgactttgag atatgcgaaa
     1261 cgttcgacaa acgatgccca agcctcatcg gaaaaatttt ctttcacatg gattttggaa
     1321 ctggtttcta ccttcgccag aaaaccttcg gtatccaact cgctgcggct gacccccaaa
     1381 atacgccctt cgggatgaag cagaccggca acatgcgcct ggtacagaca gggcaacagc
     1441 ttgcgcatcg ccaaatcgcc ggtcgcaccg aacaacacca aatcaaaatt tgtttgtgta
     1501 ctcatcgtat tatctcgtca ggaaagaatt tttcgatgcc gtctgaaacc tgtttccccc
     1561 atcacgctgc atcgcaatat cggaaacaaa ggcaggcggc ataatgagta gtaatactac
     1621 acaccgctac actttttgtc tattcccaat ttttacaatt tatttgacct agtccaaaaa
     1681 tcgggcaggt ttcccctatt ccgttacaac aatcgaaaga ttctgcgatt taaatcaaat
     1741 ttcttttcga tgtctgattt ttttgtaaca aaattacaaa ttttgtacta taataacacc
     1801 cgcttcccac tttcagacgg tatacctttt aaaatatagt ggattaacaa aaatcaggac
     1861 aaggcgacga agccgcagac agtacagata atacggcaag gcgaggcaac gccgtactgg
     1921 tttttgttaa tccactatac tcaccgtctg aatacccgat acaaaaatca gaaacgcaca
     1981 aacaaatccc caataccccc cgttccgaca ggagaccgac cgtgaacact actcctatcc
     2041 actccaaact cgccgaaatc accgggcgca ttattgaacg cagccgtccg acgcgtgaaa
     2101 aatatctggc gaagatccgc agtgccaaac aaatgggacg cttagagcgc aaccagctcg
     2161 gttgctccaa cttggcgcac ggctatgctg ccatgcctaa aagtatcaaa atcgaaatgc
     2221 ttcaggaaac cgtccccaac ttaggcatca tcaccgccta caacgacatg gtttccgcac
     2281 accagccgtt taaagacttc cctgaccaaa tcaaagacga agcgcagaaa aacggcgcga
     2341 ccgcccaagt cgccggcggc acacccgcca tgtgcgacgg catcacgcaa ggctacgccg
     2401 gtatggaact gtcgctgttc tcccgcgacg tgattgccat gagtaccgcc gtcgggctgt
     2461 cgcatcaaat gtttgacggc agcctgttta tgggcgtatg cgacaaaatc gttccgggtt
     2521 tgatgatagg cgcgctttcg ttcggtcata ttccgggtat cttcgtcccc gcaggcccga
     2581 tgtccagcgg tatcggcaac aaagaaaaag cccgcacccg ccagcttttc gccgaaggca
     2641 aggtcggacg caacgaactt ttgaaaagcg aaatgggttc ttaccacagc ccgggcacct
     2701 gcactttcta cggcacggca aactccaacc aaatgatgat ggaaatgatg ggcgtgcacc
     2761 tgcctgccgc cgccttcgtc cacccttaca ccgacctgcg cgaagcccta acccgctacg
     2821 ccgccggaca cctcgcgcgc ggcatcaaaa acggcacgat taaacctttg ggcgaaatgt
     2881 tgaccgaaaa atcctttatc aacgccttga ttggcctgat ggcaaccggc ggttcgacca
     2941 accacaccat gcacctcgtt gccatggcac gcgccgccgg cgtgattttg aactgggacg
     3001 acttcgacga aatttcctcc atcatcccgc tgctcattcg cgtttatccg aacggcaagg
     3061 ccgacgtgaa ccactttacc gcagcgggcg gactgccttt cgttatccgc gaattgctga
     3121 atgcaggcct gttgcacgac gatgtcgata ccgtcgtcgg acacggtatg cgccactaca
     3181 ccaaagagcc tttcctcatc gacggcaaac tcgaatggcg cgaagccccc gaaaccagcg
     3241 gcaacgacga catcctgcgt aaagctgaca acccgttctc ccccgacggc ggtctgcgcc
     3301 tgatgaaagg caacatcgga cgtggcgtga ttaaagtgtc cgccgtgcgc gaaggctgcc
     3361 gcattattga agcgcctgcc atcgtgttca acgaccaacg cgaagtgttg gctgcgtttg
     3421 aacgcggcga gttggaacgc gattttgtgt gcgtcgtccg ctaccaaggc ccgcgtgcca
     3481 acggtatgcc cgaattgcac aaactgaccc cgcctttagg catcctgcaa gaccgcggct
     3541 tcaaagtggc gctgctgacc gacggccgta tgtccggcgc gtccggcaaa gttccagcct
     3601 ccatccacat gacacccgaa gccctgatgg gcggcaacat cgccaaaatc cgtaccggcg
     3661 acctgatccg cttcgactcc gttaccggcg aactcaacgt cctgattaac gaagccgaat
     3721 ggaacgcccg agaagtcgaa agcatcgact tgggcgcgaa ccaacaaggc tgcggccgcg
     3781 aacttttcgc caacttccgc agcatgacca gcagcgccga aaccggcgcg atgagcttcg
     3841 gcggcgaatt tgcctgatgc gcgtttcaga cggccttttc agaccgaagg ccgtctgaaa
     3901 aattattcaa gtgttttaag atagacgtag gtcggattct cgaatccgac acagccgtcc
     3961 aagatgtcgg tttcttgaat ccgacctaca acctgtccca tcttaataaa ataccccatc
     4021 ccacccggag aaccgaaatg tccaaactga ccccccgcga aatcctgacc gccggcgcag
     4081 ttgtaccggt aatggcgatt gacgacttga gtactgccgt cgatttgtcc cacgcccttg
     4141 tcgaaggcgg catccccacc ctcgaaatca ccctgcgcac ccctgtcggc ctcgatgcca
     4201 tccgcctgat tgccaaagaa gtgcccaacg ccatcgtcgg cgcgggtacg gttaccaacc
     4261 ctgaacagct caaagccgtc gaagacgcag gcgcggtttt cgccatcagc ccgggtttgc
     4321 acgaatccct cgccaaagcc ggccacaaca gcggcatccc cctgattccc ggtgttgcca
     4381 ccccgggcga aatccaactg gctttggaac acggcatcga caccctcaaa ctcttccctg
     4441 ccgaagtcgt tggcggcaaa gccatgctca aagccctgta cggcccttac gccgatgttc
     4501 gcttctgccc gacaggcggc atcagcctcg ccaccgcgcc cgattacttg gcactgccca
     4561 acgtcctgtg cgtcggcggc tcatggctga cgccgaaaga agccgtgaaa aacaaagact
     4621 gggacaccat cacccgtctc gccaaagaag cggcggcatt gaaacccaaa gcctgaaccg
     4681 catcgtaaaa atgccgtctg aagccctttc cagtctcaga cggcattttg ccgcttggcg
     4741 gcgcagtcgg catacacggc agcaccactc cgacatactg cccctaaaat gcccatccgc
     4801 cttccgcata ataaaaataa cgttcagttc attcgacagc agccggacag cccatactac
     4861 gcggctgaaa aaatgccgtc tgaaacgcat tcagacggca tccgcttaaa aaaacaactg
     4921 attcaaggcc gattaatccg cttccaaaac cactttcatc gcttgatttt cggcggcgtg
     4981 tttgaacacg tcgtaggctt tttccaattc gctgaatttg aaacggtggg tcaacatttt
     5041 ggtgtaatcg acggagctgc tggaaattgc cttcatcagc atttcggtgg tattggcgtt
     5101 taccaggccg gtagtgatgg caagcttttt aatccacagt ttttccagtt tgaaatcaac
     5161 ggattgaccg tgtacgccga caacggcgat atggccgccg ggtttcacaa tgtcttggca
     5221 catattccaa gttgcaggga taccgacggc ttcgatcgcg cagtccacgc cgtcttcgcc
     5281 gacgatagcg acaacttgct tgcttacatc gccggaagca gggttaatgg tatgggttgc
     5341 gcccaactct ttcgccagtt tcaaacggtt ttcgtccata tcgcaaacaa tgatggcggc
     5401 ggggctgtac agttgggcgg tcggcaaggc ggacataccg acaggagcgg cacctgcgat
     5461 gaatacggtg tcgccgggtt tgacatcgcc gtattgcacg ccgatttcgt gtgcggtcgg
     5521 caaagcgtcg ctcaacaaca gggcgacttc ttcgttgacg ttgtcgggca gcggcacgag
     5581 gctgttgtcg gcataaggcg tgcggacgta ttctgcctgc gtgccgtcaa tcatgtagcc
     5641 caaaatccag ccgccgttgc ggcaatgcga ataaagttgg attttgcagt tgtcgcaagt
     5701 acagcatttg ctgacgcatg aaataatgac tttatcgccg actttgatgt tttttacagc
     5761 ctcgccgact tcttctacaa taccgatgct ttcatgaccg agaatacgac cgtcggcgac
     5821 ttcgggtttt tttgcctttc caaataccca aatcggtacc gcaaatcgtg gttttgacga
     5881 ttttcaccac cgcatcggtc ggatcgataa tctgcggacg gggtttttct tcaaaacgga
     5941 tgtcgtttgc gccgtgataa accattgctt tcatgctgat actccttgct tgttgataaa
     6001 taatttcaat accgcaataa agtttcttta tatgagttat atgcccctac aaaaaataag
     6061 tcaataagaa ttattttcac gatgttatac aataacatac tgttttaaat ataaataaaa
     6121 ccaccgattg atattaatga acacacccat ccccttctcc gaacggctca tccgctggca
     6181 aaaacaacac ggtcgccacc acctcccttg gcaggtcaaa aacccttatt gcgtctggct
     6241 ttccgaaatc atgctccagc aaacgcaggt cgccaccgtg ttggactact atccgcgttt
     6301 cttagaaaaa ttcccgaccg ttcagacgct tgccgccgcg ccgcaagacg aagtgttgtc
     6361 gttgtgggcg ggcttgggct attacagccg cgcgcgcaac ctgcacaaag ccgcgcaaca
     6421 agtcgtcagg caattcggcg gcacgtttcc gtcggagcgc aaagacttgg aaaccctctg
     6481 cggcgtaggc agaagcaccg ccgccgccat ttgcgccttc tccttcaacc gccgcgaaac
     6541 cattttggac ggcaacgtca aacgcgtact ctgccgcgtg ttcgcccgcg acggcaatcc
     6601 gcaggacaaa aaatttgaaa actcgctctg gacacttgcc gaaagcctgc tgccgtctga
     6661 aaacgccgat atgcctgcct atacacaagg tttgatggat ttgggcgcga ccgtgtgcaa
     6721 acggacgaaa cccttgtgcc accaatgccc gatggcggac atctgcgaag cgaaaaagca
     6781 aaaccgcacc tccgagctgc cgcgcaaaaa aaccgccgcc gaagtaccga ccctgccgct
     6841 ttactggctg attatccgca accggaacgg cgcgattttg ctggaaaaac gccccgccaa
     6901 aggcatttgg ggcgggctgt attgcgtgcc gtgttttgaa agtttgaacg ggctttccga
     6961 ctttgccgcc aaattctccc tgaccatggc agatatggac gaacaaaccg ccctgaccca
     7021 ccggctgacg caccggttgc tgatgattac gccgtttgaa ggacaaatgc cgtctgaaag
     7081 cccttcagac ggcatttgga taaagccgac gcatttgaaa gattacggcc tgcccaagcc
     7141 tttggaaatt tatttaaacg gtaataggtt agaataaaca aaataaaccc attgaactgt
     7201 tgtttgcagg tatcgcagca agaacaaccg atgaatttgg gtcgtatttt aggcggcggg
     7261 ataatgttca aatgggacat ttggaacgga agaagtcggc aatttaaaaa ggatttaaaa
     7321 agcaaagaag gtcaaaaaca tgaacacaaa cttaaatgac aaagacaaag ccatggatac
     7381 cgcaatcagg tttcagaaaa ggatgaggat tccgaaattt ttctttttaa ttctctgaat
     7441 cacaatggtt ttggcattta tccaagacgt gataacgggt tctaattttc tgcaaataac
     7501 aattaatgta aaattttcgt aaaaatttat cggcttttaa aacaaaattg actaaaatag
     7561 tcgcgagttt ttactgcaat aaaggagatt gcaatgaata tgaaaacctt attagcacta
     7621 gcggttagtg cagtatgttc agttggtgtt gcgcaagcac acgagcataa tacgatacct
     7681 aaaggtgctt ctattgaagt gaaagtgcaa caacttgatc cagtaaacgg taacaaagat
     7741 gtgggtacag tgactattac tgaatctaac tatggtcttg tgtttacccc tgatttacaa
     7801 ggattaagcg aaggcttaca tggtttccac atccatgaaa acccaagctg tgagccaaaa
     7861 gaaaaagaag gtaaattgac agctggttta ggcgcaggcg gtcactggga tcctaaaggt
     7921 gcaaaacaac atggttaccc atggcaagat gatgcacact taggtgattt acctgcatta
     7981 actgtattgc atgatggcac agcaacaaat cctgttttag caccacgtct taaacattta
     8041 gatgatgttc gcggtcactc tattatgatc cacacgggtg gtgataatca ctccgatcat
     8101 ccagctccac ttggcggtgg cggcccacgt atggcatgtg gcgtgattaa ataattcgat
     8161 tgttcgaaac gaaaagtgcg gtgaattttg accgcacttt tttgctagat atttagcatt
     8221 gagacctttg caataacata ggt
//
LOCUS       DAXHSJ010000088         8150 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000088 DAXHSJ010000000
VERSION     DAXHSJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.088"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(266..1651)
                     /gene="pntB"
                     /locus_tag="ACFM8U_001348"
     CDS             complement(266..1651)
                     /gene="pntB"
                     /locus_tag="ACFM8U_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC6334583.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALGVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKSANSVIITPGYGMAVAQAQYPVAEITELLHKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1717..2100)
                     /locus_tag="ACFM8U_001349"
     CDS             complement(1717..2100)
                     /locus_tag="ACFM8U_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC6334584.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKTGGFRFSDNHHPRDFLARTQ
                     GAAARAHAAQQNSFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2104..3645)
                     /locus_tag="ACFM8U_001350"
     CDS             complement(2104..3645)
                     /locus_tag="ACFM8U_001350"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC6334585.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKATVWACPLIYKVNAPSEDELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSDDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFKKG"
     gene            3935..4768
                     /gene="serB"
                     /locus_tag="ACFM8U_001351"
     CDS             3935..4768
                     /gene="serB"
                     /locus_tag="ACFM8U_001351"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC6334586.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SGADAALLGSRQIDHAVLPDMNFDELGLIVSDMDSTLITIECVDEIAEGVGLKNKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPSAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGVGVAYRAKPKAQAAADACINFGGLERVRGLF
                     R"
     gene            4832..6055
                     /locus_tag="ACFM8U_001352"
     CDS             4832..6055
                     /locus_tag="ACFM8U_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6334587.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGVY
                     TSFREGVAQASGMRRVAVLTLCGAVAGVGWCLLKALGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGAAAVFFQRTAQKFPFIKRDNIKIIPLAVCMFVLIGAIAVWFP
                     EILGNGKAGNQLTFSGLTGWQYALELAAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPAMSSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6216..7796
                     /gene="purH"
                     /locus_tag="ACFM8U_001353"
     CDS             6216..7796
                     /gene="purH"
                     /locus_tag="ACFM8U_001353"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC6334588.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDVYPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         1795 a         2202 c         2324 g         1829 t
ORIGIN      
        1 caagtggaaa aatacttggc agattatcag gcacacttgg aaaaagtgtt cggctaatta
       61 aaaatccatc ttcaacacgg agatggattt tgtttgtttc gttgattttg tgtcagtttc
      121 agatgtaggt gcttattcgg acgggccgtc tgaaaatgtt tgccccaatg caaaaaaaat
      181 cactgcaaac cttcataaac ggggtttgca gtgatttttt caaatcaaac agattgaaaa
      241 cctgcgccga attgttcaga cggcattatt ttttcagttc ggacagaatg tcatctacgg
      301 ttttcttcgc atctccgaaa cacattacgc tgttttcgtt gaagaacagc gggttttgta
      361 cacctgcgta gccggtattc atcgagcgtt tgaagacgac gacttctttt gccttccata
      421 cttccaacac gggcataccc gcaatcgggc tgttcgggtc ggtttgggcg gcaggattga
      481 cggtatcgtt cgcaccgatg accaagacca catcggtttc ggggaaatcg tcgttgattt
      541 cgtccatttc caacacgatg tcgtagggga ctttggcttc ggcaagcagt acgttcatat
      601 gaccgggcag gcggccggcg acggggtgga tgccgaagcg tacttctgtg ccgtttttat
      661 gcaaaagctc ggtgatttcg gcaacggggt attgcgcctg tgcgactgcc atgccgtagc
      721 ccggggtgat gatgacgctg tttgcgcttt tcagcatttc ggcaacatcg gcagctttga
      781 cttctcggta ttcccctatc tcttggctgc cggaagatga tgtgccgctg tcgctgccga
      841 aaccgccggc aattaccgag acaaacgagc ggttcatggc tttgcacata atgtaggaca
      901 gaatcgcgcc gcttgagcca accagcgcgc cggtaacgat gagcaggtcg ttggagagca
      961 taaagcctgc cgctgcggcc gcccagccgg agtaggagtt gagcatggac acgaccacgg
     1021 gcatatctgc gccgccgatg gaggcaacca agtgccagcc gaatgcgagg gcaatcaggg
     1081 tcataatcag caggatgaag ccgctgccgt caatgccgac gaatacgagc agcaacacaa
     1141 acgatacgcc aagtgccagt gcgttgagct tgtgtttggc gggcagttgc agcgggctgc
     1201 tgctgatttt gccgttgagc ttgccgaatg cgaccagcga gccggtaaag gttaccgcgc
     1261 cgatgaaaat gcccaaatag acttcgacca gatggatggt gtgcatatcg tgcgaaacgt
     1321 tgcccggcgc gatatagctg ttgaagccga ccaaaaccgc cgctaggccg acgaagctgt
     1381 gcagcagggc aatcagttcg ggcatttcgg tcatttccac ctttttggct ttgtagatgc
     1441 cgattgccgc gccgatgagc atggcgatga tgatccagcc cagtccgtgg gtattgtcgg
     1501 aaaaaacagt tacaaaaagg gcgaccgcca taccggcgat accggaatag cagccctgtt
     1561 tggcggtttc ctgtttggac agccccgcca gtgagaagat gaataaaatt gcggcaacga
     1621 tatacgccgc tgttacgagt cctgaagaca tggaaattct ccgattttcg atgatttgtt
     1681 tttaatgccg tctgaactgt tcagacggca tctgtttcaa gcagccgcga caaacagccc
     1741 gacggtgcag gcaaatccgc ccgcccacat cagcgagcgc aatgctgctt tgtctgcgat
     1801 gtagcaccag ataaaggcga ggcggaacag gatgaacagg ccggcaagcg tgttgacggt
     1861 tgcttgtccg gcattgccgg ttgcgtgtgc cgtcaaaacg gcggctgcaa atggtgcaaa
     1921 tgcttcaaaa ctgttttgct gcgcggcatg ggcacgggca gcggcacctt gggtacgcgc
     1981 cagaaaatca cggggatgat gattgtcgct gaagcggaat ccgccggttt ttttggcata
     2041 cgctgcacaa aaaagcggca ataagcaggc aatcaggatg caccaatagg cgaaagtcat
     2101 ggcttaccct ttcttaaaca tattcaacat gcggcgcgtt acgaagaagc cgccgaagat
     2161 attgatgctg gcaatcagga tggcgatgaa ggcaagcagg gtaacgaaac cgttgccttg
     2221 gctgatttgc agcagcgcgc cgacgacgat gatgccggag atggcgttgg ttaccgacat
     2281 cagcggtgtg tgcagcgagt ggctgacgtt ccaaacgacg tagtacccga tgacgcaggc
     2341 gaggacgaac acgataaagt ggttcaggaa tgctgcgggt gcgaccgcgc cgacccacag
     2401 caccaatacg gcggcgatga cggcgggcgc gagttttttc cacagggaaa caggtttcgg
     2461 ctcgggcttg gcggcaggcg cggctttttc agacggcgtt tgctgcggct gggcggaaac
     2521 ttgaatcggc ggaggcggga aggtgatttc gccgtcgtgg gtaacggtca tgttgcggat
     2581 aatcacgtct tcgaagtcca acgtgatttc gccgtctttg ttcgggctta acagcttggt
     2641 caggttgacc aagttggtgg cgtaaagctg ggaagactgt ccggcaaggc ggtttgccat
     2701 gtcggtgtag ccgatgattt tcacgccgtt gccggttacg gacaattcgc ccgggcgggt
     2761 gagttcgcag ttgccgcccg tcgccgccgc caaatcgacg atgacggagc cggatttcat
     2821 gctttccacc atttctttgg taatcagctt gggcgcgggt ttgcccggaa tggcggcggt
     2881 ggtgatgatg atgtccactt ctttcgcctg ctcggcaaag agcttcatct cggctgcgat
     2941 aaattcgtcg ctcatcactt tggcgtagcc gtcgtcgctg ccgcccgatt cttgtgggaa
     3001 gtcgagtttc aggaacttgc cgcccatcga ttcgatttgt tccgccactt ccaagcgggt
     3061 atcgaacgcg cgtaccactg cgccgagcga gtttgccgta ccgatcgccg ccaaacctgc
     3121 cacacctgca ccaatcacca aaacctgcgc gggcggcact ttgccggcgg cggtaatttg
     3181 accggtgaag aaacggccga aggcgttggc ggcttcaatt acggcgcggt agccgctgat
     3241 gtttgccatc gaagacaaag cgtccaaagc ctgcgcgcgc gaaatacggg gcaccatgtc
     3301 cattgccagc gcgttgactt tcttggcgcg caaggcttcg accaaagcct cgttttggcg
     3361 cggccacagg aagctgacga tggtctgtcc ttctttgagc agcggcagct cgtcttcgga
     3421 cggcgcgtta accttataaa tcaaagggca tgcccaaacc gttgctttgt cggcaacggt
     3481 cgcgcctgct gtttggtaag cggcatcgtc caaacttgcc gccaaacctg caccgctttc
     3541 gacaacggtt tcaaagccca gtttgcccag cagggcaacg gtggcgggcg tgcaggcgac
     3601 gcgggtttcg ccggataatg actcacgtgg gataccgatt ttcatctctg aatccttttt
     3661 cgggttgttt atatgtatcg tgggttaaat ttaaatcgtg gcggggcgga gcaacgccgt
     3721 accggtttaa aaccgattca cttcaaatgt taatatattt tagataatcc ccttataacg
     3781 aattttcatc aggctggcaa tagttgcggc attttcccgt gttgtccgac acatactgcc
     3841 gtttgcgttt gaagtcattg tccgattgca gtccctcccg ccgtaacatt ctttgccgtt
     3901 tcactatata atccgcattt tttgagccgc cgttatgccg cacgccctcg tcctccaatt
     3961 tccctccgcc gaagccctgc cttccgactt cccctcccgc ctgcccgaac ctgattgcgc
     4021 cgatgaaaag cgtatgcgtt ttatcgttga agaagggttt tctttaagcg gggcggacgc
     4081 ggcgttgctt ggcagccgtc aaatcgacca cgccgtgttg ccagatatga attttgacga
     4141 actcggttta attgtcagcg atatggattc gacactgatt accatcgaat gcgtcgatga
     4201 aattgcggaa ggcgtgggtt taaaaaacaa agtagcggaa attaccgagc gttcgatgcg
     4261 cggcgaactc gatttcgaac agtctttacg cagccgcgtc gcgctgttgg cgggattgga
     4321 cgaacagatt ttggcggacg tttatgaaaa cgttttgaag ctctcgccca gtgcggaatt
     4381 tttgttggac gaatgcaaaa ggcacaatgt gaaattcctg ctggtgtccg gcgggttcac
     4441 gttttttacc gaaaggctgc aacaacgcct cggcttcgaa taccaacacg ccaatatttt
     4501 ggaaattgaa aacggcaggc tgaccggacg tttgaaaggc agaattatcg acgcgcaggc
     4561 aaaggcggat ttattgcgcg aataccgcag ccgcctcgga ttgcagccgc atcaggtgtt
     4621 ggcgatgggc gacggtgcga acgatattcc gatgctcaaa gaagcgggcg tgggcgtggc
     4681 ttaccgcgcc aaaccgaaag cgcaggcagc cgccgatgcc tgtatcaatt tcggcggttt
     4741 ggagcgtgta cgcggcctgt tcaggtaggc ggataggaaa cggatgccgt ctgaaaggtg
     4801 ttcagacggc atttgaacgg caggaacgac agtgggacgc agaaaacttt ggtttgccct
     4861 ggcagcagcg ggcgttatcg gcggtttggt cggcattgtg ctgacggaac tgatgcactt
     4921 catacagcat acggcatacg gttatggcgc ggacggcgtg tacacttcgt tccgcgaagg
     4981 tgtggcacag gcttccggta tgcggcgcgt tgccgtgctg acgctgtgcg gcgcggtcgc
     5041 aggagtcggc tggtgcttgc tgaaagcctt gggcaagccg caaatcgaaa tcaaagccgc
     5101 cttgaagcag ccgttgcagg ggctgccgtt tctgacgacg gtttttcatg ttctgctgca
     5161 aatcataacg gtcggactcg gttcgccgct cggacgggaa gtcgcgccgc gtgaaatgac
     5221 tgccgcgttt gcttttgccg gcggcaggcg gttgggtttg gatgaagacg aaatgcgcct
     5281 cttgattgcc tgtgcgtccg gcgcgggttt ggcagccgtg tataatgtgc cgctcgcctc
     5341 cacacttttc atcctcgaag ccatgctggg cgtgtggacg cagcaggcgg ttgccgccgc
     5401 gttgctgact tccgtcattg ccaccgccgt ggcgcgtatc ggcttaggcg acgtacagca
     5461 gtatcatccc gccaaccttg ccgtaaatac ttcattgctt tggttttccg ccgtcatcgg
     5521 cccgatattg ggcgcagccg ccgtcttttt ccagcgtacc gcccaaaagt tcccctttat
     5581 caagcgcgac aacatcaaaa ttattccctt ggcagtctgc atgtttgtgc tcatcggcgc
     5641 gattgccgtg tggtttcccg aaattttggg caacggcaaa gcaggcaacc aactgacttt
     5701 tagtggtttg accggttggc aatacgcttt ggaacttgca gccgtcaaat ggctggtcgt
     5761 cttgatggcg ctggcggcag gcgcatacgg cggcctgatt acgccgtcta tgatgctcgg
     5821 cagcaccatt tcttttgcag ccgccgccgc gtggaacagc gttttccccg caatgtcgtc
     5881 tgaaagcgcg gcagtcgtcg gagcggcggt gtttctcggc gtgtcgctca aaatgccgct
     5941 gaccgccatt gtgttcgtat tggaactgac ctatgcgccg ctgtccctgc tgctgccttt
     6001 gtgcatcggc atggcggggg cattgggaac atcgggaaaa atgggcttca aatgaatgcc
     6061 gacaaatgcc gtctgaaagg ttttcagacg gcatttcttt gtaaattgtc agcaatatct
     6121 gcaaaaacct actgccaaaa aactagaaat agcggataat accgccccga aaaagttccc
     6181 ctttccccat ccaactgtca gaaaaggaag ccccaatgtc cgtcatcaaa cgcgccctga
     6241 tcagcctgtc cgacaaggca ggcgcggtcg aatttgccca aactctgcac aagctcggtg
     6301 tcgaaatcct ctccaccggc ggcacggcga aactgcttgc cgatgcgggc gttcccgtga
     6361 ttgaagttgc cgactatacc ggcttccccg aaatgctcga tggccgcgtg aaaaccctgc
     6421 atccgaaaat ccacggcggc atcctcggtc gtcgcgattt gggcgaacac gtcgccaaga
     6481 tggaagaaca cggtatcggc aacatcgacc tcgtgtgcgt caacctttac cccttcgccg
     6541 ccaccatcgc caaaccaaac tgcacactgg aagacgcgat tgaaaacatc gacatcggcg
     6601 gcccgactat ggtgcgttct gccgcgaaaa actggaaaca cgtcgccatc gttaccgata
     6661 ccgccgattt ccctgccatc gccgccgaac tcgaagccaa caacggcgca ttgagcgaca
     6721 aaacccgttt caacctctca cgcaaagcat tcagccatac cgcccaatac gacggcatga
     6781 tttccaacta cctgacctcg ctttcagacg acgtcttgag cggagagccc caaatcggcg
     6841 aattcccgag ccagttcaac caaagctgga ttaaagtgca agatatgcgc tacggcgaga
     6901 atccgcacca acgcgccgcg ttctaccgcg atgtgtaccc cgccgcaggc agcctttccg
     6961 cctacaaaca gctccaaggt aaagaattgt cgtacaacaa catcgccgat gccgatgccg
     7021 catgggaagc cgtcaaatcc tttgacgcgc ccgcctgcgt gattgtgaag cacgccaatc
     7081 cgtgcggcgt cgccgttgca gccgatacct tgaccgccta caaactcgcc tacgccaccg
     7141 acaccaccag cgcgttcggc ggcatcatcg ccttcaaccg cgaagtggac ggcgaaaccg
     7201 tcaaacagat tacagacaac cagtttatgg aagtcctgat ggcgccgaag ttcaccgccg
     7261 aagccctcga aatcgccgcc gccaagaaaa acgtgcgcgt gttggaagtg cctttggaag
     7321 caggcgcgaa ccgctttgaa ctcaaacgcg tcggcggcgg attgttggta caaacgcccg
     7381 acatccaccg catcagccgc gccgatttga aagtcgtctc caaacgccaa ccgaccgaac
     7441 aggaatggaa cgatttgctg ttcgtttgga acgtcgcaaa atacgtcaaa tccaacgcca
     7501 tcgtcttcgg caaaggcggt caaacctacg gcatcggcgc aggccaaatg agccgcgtgg
     7561 acagtacccg catcgccgcc cgcaaagcgc aggacgcagg cttggactta aacggcgcat
     7621 gtgccgcctc tgatgctttc ttccccttcc gcgacggcgt ggacgtgatt gccgagcagg
     7681 gcatcaaagc catcatccat ccggcaggtt cgatgcgcga tcaggaagtc ttcgacgcag
     7741 cggacgaaca cggcatcgcc atggtcgtaa ccgacgtgcg ccacttccgc cattaacgca
     7801 gatgaacaag gcaatgccgt ctgaagggct ttcagacggc attttgcgct attttgtgaa
     7861 ggtagggatg acggttcggg tattcctgac agggtggatt ttcaaggtgt tgtatagggt
     7921 gtaggaggat tcgtaaaagg tgggatgcag ggtgtgcttc agcccgctgc atcaaaaatt
     7981 tttggagaat cggcgggagt cggcggtttt ggtttcggcg gggacggtgg aaatgggtaa
     8041 cattgacgga atcgacggaa gcggtgggct tcagcccacc gcttgtatat tggaatcacc
     8101 gtatcatagc gccacccgcg cccacccaag gcacacaacc gttgcgtagc
//
LOCUS       DAXHSJ010000089         8147 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000089 DAXHSJ010000000
VERSION     DAXHSJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.089"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            111..1052
                     /gene="miaA"
                     /locus_tag="ACFM8U_001354"
     CDS             111..1052
                     /gene="miaA"
                     /locus_tag="ACFM8U_001354"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC6334589.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEANACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIERALEVYHLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRLYPSLTADSPAIRCVGYRQAWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            1056..1775
                     /gene="tadA"
                     /locus_tag="ACFM8U_001355"
     CDS             1056..1775
                     /gene="tadA"
                     /locus_tag="ACFM8U_001355"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC6334590.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLDGTPPQEMSQAHKARLLAELKNHPPVAAFPQQGEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIAAAHNTCIADCNVSRHAEINALAQASSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1780..2268
                     /locus_tag="ACFM8U_001356"
     CDS             1780..2268
                     /locus_tag="ACFM8U_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC6334591.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGMFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2334..3086
                     /gene="rlmB"
                     /locus_tag="ACFM8U_001357"
     CDS             2334..3086
                     /gene="rlmB"
                     /locus_tag="ACFM8U_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC6334592.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(3148..4539)
                     /locus_tag="ACFM8U_001358"
     CDS             complement(3148..4539)
                     /locus_tag="ACFM8U_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFC6334593.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATVMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(4554..4808)
                     /locus_tag="ACFM8U_001359"
                     /pseudo
     CDS             complement(4554..4808)
                     /locus_tag="ACFM8U_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4846..5721
                     /gene="dapA"
                     /locus_tag="ACFM8U_001360"
     CDS             4846..5721
                     /gene="dapA"
                     /locus_tag="ACFM8U_001360"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC6334594.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            5730..6926
                     /gene="bamC"
                     /locus_tag="ACFM8U_001361"
     CDS             5730..6926
                     /gene="bamC"
                     /locus_tag="ACFM8U_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC6334595.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSHAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(6983..7915)
                     /gene="pip"
                     /locus_tag="ACFM8U_001362"
     CDS             complement(6983..7915)
                     /gene="pip"
                     /locus_tag="ACFM8U_001362"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC6334596.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIQHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDIFPRLL"
BASE COUNT         2091 a         2686 c         1911 g         1459 t
ORIGIN      
        1 aaaggcatac ggtaaacacg cccccttgcc gtcggcgtgc ttccgcaaac caaaatataa
       61 cgtcggcacg ctttccgcta taatccgtat ttttccaaac ctgaaacgtt atgccgaccc
      121 cgaaagcctt tgccctgctc gggccgaccg ccggaggcaa aaccgcgctc gccctcaaaa
      181 ttgccgaaac cctgcctgtc gaaatcatca gcctcgattc cgcgctggtt taccgcgata
      241 tggacatcgg cacggcgaaa ccgtccgcct ccgaacgcgc ctttgtcccg caccacctta
      301 tcgacatcat cccgcctacc gaatcataca gcgccgcccg ctttgtcgaa gactgcacgc
      361 gcttggtcgg ggaaatttcc tcacgcggca ggtttgccct tattgtcggc ggcacgatga
      421 tgtatttccg cgccctgacc caaggtttga acgatttgcc cgaagccaac gcctgcctgc
      481 gtgccgactt ggacgaacaa aaacagatgt acggcttgga tttcctctac cgcaccctgc
      541 aaaaagtcga ccctgaaacc gcatgccgtc tgaaaccgaa cgacagccag cgtatcgaac
      601 gcgctttgga agtttatcat ctgaccggca aaccgatgag cgaacacctc ggcagacaaa
      661 gcccccatac gctccccttc gatttgcata ccgccgccct gattcccgaa aaccgcgccc
      721 gcctgcatga aaatatcgcc ctgcgtttcc acctgatgct tgaacagggc tttatcggcg
      781 aagtagaaaa cctgcgccgc ctctatccca gcctgaccgc cgactcccct gccatacgct
      841 gcgtcggcta ccgtcaggcg tgggaacacc tcgacggcgc aaccgacaga caaaccttca
      901 tcgaaaaagg cattgccgcc acgcgccaac ttgccaaacg ccaactgact tggttgcgca
      961 aaacaccttt agactgcgtt gccgacccgt tttcagacgg cacttcatgc acacgcctga
     1021 ttgaggcggc aaaacggttt ttcggcgcat aaaccatgct gaccacaccg ccgctcgccc
     1081 ccaaaaccgt cgccgccctg cacaggctcg gtatccgtac gctcgaagaa ctgcggcaaa
     1141 acggttcggt caaagccttt ctgctgctca aagcatcggg tttgaccctc acgaaaagca
     1201 cgctgtggca actcgagtcg ctgctggacg gcacgccgcc gcaagaaatg tcccaagcgc
     1261 ataaagctcg cctgcttgcc gaattgaaaa accatccgcc cgtcgccgcc tttccgcagc
     1321 aaggcgaaat ggaacacttc atgcgcgaag cactgcgcca agcagaacaa tccgccgccg
     1381 acggcgaaat acccgtcggg gcagtcatcg tttcagacgg caaaatcatc gcggcggcac
     1441 acaacacctg cattgccgac tgcaatgtca gccgacacgc cgaaatcaac gccttggcac
     1501 aggcaagcag cgaaatgcaa aactaccgcc ttgacggatg cgatatctac atcaccctcg
     1561 aaccctgcgc catgtgcgcg tccgcactga tacaggcacg aatcaggcgc gtgatatacg
     1621 gtgcggcaga acccaaaacc ggcgcggcag gcagcatcgt caacctgttt gccgacaaac
     1681 gcctcaatac gcacaccgcc atacggggcg gaatcctgca agaagaatgc cgcgccgtat
     1741 taagccgttt ttttcagaac aaaagaaaag gttgaatcca tgaccgccct actcgtcatc
     1801 ctcgccctcg ccctgatagc cgtcggtacg gcgggcatcg tttaccccgc cctgcccgga
     1861 ttggcattga tgtttgccgg aacatggctg cttgcctact ccggcggcta ccaaatctac
     1921 ggcgcgggcg ttttgtggac ggtcggactc atcagccttg ccggcatact ggcggactat
     1981 gtggcaggca tatgggggac aaaatatacc ggagcgggca agctcgccgt tcgcggcgca
     2041 ttggccggca gcatcatcgg tatgtttttc tcccttcccg gactaatact cggccccttt
     2101 atcggcgcgg cggcaggcga actgatcgat cggcgcaata tgcttcaggc aggtaaagcg
     2161 ggcttgggta cgctgttggg gcttgtcgtc ggtacggcat tcaaaatcgg ctgcgccgta
     2221 tccatcttgt ttatcctgtt ggtgaaatac atcgcatacc tgttttaacc ccttcagacg
     2281 gcatcaggat aaattgctat aatgccgtct gaaatttccg acaaagcacc atcatggcaa
     2341 accaaagacc tatctacggc ttccacgccg tcaacgcccg attgtggcaa aaccccaaat
     2401 ccatcgtcga actctacatc caagaaggca aatcagacgc acgcacgcgc gaagtgttgg
     2461 aaaaggcggc aaacgaaaac atccgcgtat attttgccga tgccgaccgc ctcaacgcca
     2521 tcagcaaagg cgcgcgccat cagggtgtgg tcggatttat cgatgcctcc aaaaaccacg
     2581 tccacctcga agacgtattg gaaaacctca gcgaaccgcc gctgttgctg atactcgacg
     2641 gcatcaccga tccgcacaac ctcggcgcgt gcctgcgtac cgccgacgca atgggcgtac
     2701 acgccgtcat cgcaccgaaa gacaaaagcg cggggctgaa cgccaccgtc agcaaagtcg
     2761 ccagcggcgc ggcggaaacc gtcccctaca tcaccgtaac caacctcgcc cgcaccctgc
     2821 gcgagctgaa agaatacggc atttggatca tcggcaccga tatgggcggc aacgccgacc
     2881 tttaccattg cgatctgccc gacagcgcgg catgggtgat gggcaacgaa ggcgacggta
     2941 tgcgccgcct gacacgcgaa cattgcgaca tgctggtgtc catacccatg ttcggcacgg
     3001 tcgaaagcat gaacgtctcc gtgtctgccg gaatggtatt aagcgaaacc cgccggcagc
     3061 gcgtattaaa aaacgaaaaa gcgtaagcct caaaaacaat gccgtctgaa acagggattc
     3121 tgtttcagac ggcattcgtt ttcaaaatca gtgttccaag tggtcggtat caaacttgac
     3181 cgccgcctca gttttatctt cgggcaagac caaattcagc aagactgccg taatgccgcc
     3241 ggcggaaata gagttttgga acaagacggg caggttttta aacacttccg gctcaaacgc
     3301 gacacccaag cccaaaccga ccgacgttgc tgcaattacc gcttcgcgcc tgcggatgcc
     3361 gtggctgacc aaaatccgca cgcccgcaat cgcaatcaag ccgaacatca aaaccatcgc
     3421 gccgcccaac accggactcg gaatcgtcgt aaacgcgcga ccgacaacgg ggaacagacc
     3481 caacagcacc aaaatcacgg caatatattt gcccacatga cgcgaagcca cgccggtcat
     3541 ctgaatcacg ccgttgtttt gcgcaaacgt cgtcagcggc agcgaaccca aagccgtcgc
     3601 aatcaccgac accaagccgt cagccaacac gccgccgcgc aaacgtttgg tgtattcctc
     3661 gccttcaatc ggctggtcgg acaccattac cgttgcggtc aaatcgccga ccgcctcaaa
     3721 cacgctcaac aggaaaatcg cacccgccac aataaatgcg tgccagtcaa aagcaaaacc
     3781 atatttaaac ggtacgggca gcgtaaccag cggcaggttt tgcagtgccg aaaaatccac
     3841 cttgcccaaa aacagcgcga cgatatagcc ggcaatcaga ccgaccgcaa tgccgctcat
     3901 ccgcagcagc gggtttttca tgcagttgaa caccagcaca atcagcagca ccagcgatgc
     3961 cagccccaag ttttccatcg agccgaacgt gccgtccgcc tttgcgccga agccgccgcc
     4021 gaaatcggta ataccgacgt gtaccaaact caagccgatc agcatcacca ccacaccgct
     4081 gaccgtcggc gtaatcactt ttttcaaata cggcagaagc cacgccgaga aacacaccaa
     4141 aaacgcgccg acaaacgata cgcccaagag cgtcgaaatc atcgcatcct tagtcaaacc
     4201 gccctctttc atccccgcgc ccagcgcaat catgacggta acgaacgaga aattcaccga
     4261 ctggatggac agcatccccg aaccgaccgg cccgaagcgg ttgacctgca aataagtgcc
     4321 gacacccgac gcaaccatcg ccatcgacac gagatacgcc gtcatctcca ccggcagttc
     4381 cagcgcgccg cccacaatca gcgcgggcgt aatcatcggc acgaaaatcg ccaaaagatg
     4441 ggtaaccgcg ctcaacagcg cattaccgaa cggcggccta tcctccaaac cgtacaccaa
     4501 atcaggcgca tccgcccctt ttcccatatg accgctcata atgtttcctt gcttcaaatg
     4561 ttgaaaaaac caaagcgcgg attgtagcga atccgccata ccgcttcaat ttcgatttcg
     4621 gacggcatac gcgcccgtcc ggcaaacaga ttccgaaccg tcgccgccgg aatatcgttt
     4681 acaaaacgca ataacctgat ttcaaacaca aaaatacaaa cccgacgcat ccgccgtcaa
     4741 gccgccccgc tcatcccgga cccgccggat ttcacacctg cggtaaagta ggctacaatc
     4801 ggaagcatta ccgcccccta caccacaact gataaaaggt tctgtatgtt acaaggtagc
     4861 ctggttgccc tgattacccc gatgaatcaa gacggcagca tccattacga acaactccgc
     4921 gacttaatcg actggcacat tgaaaacggc acggacggca tcgtcgccgt cggcacgaca
     4981 ggcgaatccg ccaccctctc cgtcgaagaa cacaccgccg tcatcgaagc cgtcgtcaaa
     5041 cacgttgcca aacgcgtccc cgtcatcgcc ggcacagggg caaacaacac cgtcgaagcc
     5101 atcgcccttt cccaagccgc cgaaaaagcc ggggcggact acaccctctc cgtcgtcccc
     5161 tattacaaca aaccttcgca agaaggcatg taccgccatt tcaaagccgt tgccgaagcc
     5221 gccgcgattc cgatgatact ctacaacgtt cccggcagaa ccgtcgtcag catgaacaac
     5281 gaaaccatcc tgcgccttgc cgaaatcccc aacatcgtcg gcgtgaaaga agccagcggc
     5341 aacatcggca gcaacatcga actcatcaac cgcgcgcccg aaggcttcgt cgtcctgtcc
     5401 ggcgacgacc acaccgccct accgttcatg ctctgcggcg gacacggcgt gattaccgta
     5461 gccgccaacg ccgcgcccaa gctctttgcc gatatgtgcc gcgccgcgct tcaaggcgat
     5521 atcgcccttg cacgcgaact caacgaccgg ctgattccga tttacgacac catgttctgc
     5581 gaacccagcc ccgccgcacc gaaatgggcg gtatccgccc tgggcagatg cgaaccgcac
     5641 gtccgcctgc cgctcgttcc gctgacggaa ggcggacagg caaaagtccg cgccgccctg
     5701 aaagcctcag gacaacttta aatatagcca tgccgtccga accgttcgga cggcataacg
     5761 caacaaacac tttaatatcc atcacacagg atgacacgat gacccatatc aaacccgtca
     5821 ttgccgcgct cgcactcatc gggcttgccg cctgctccgg cagcaaaacc gaacagccca
     5881 agctcgacta ccaaagccgg tcgcaccgcc tgatcaaact cgaagtccca cctgatttga
     5941 acaaccccga ccaaggcaac ctctaccgcc tgcctgccgg ttcgggcgcc gtccgcgcca
     6001 gcgatttgga aaaacgccgc acacccgccg tccaacagcc tgccgatgcc gaagtattga
     6061 aaagcgtcaa aggtgtccgc ctcgagcgcg acggcagcca acgctggctc gttgtcgacg
     6121 gcaagtctca tgccgaaatc tggccgctcc tgaaagcctt ttggcaggaa aacggcttcg
     6181 acatcaaatc cgaagaaccc gccatcggac aaatggaaac cgagtgggcg gaaaaccgtg
     6241 ccaaaatccc ccaagacagc ttgcgccgcc tattcgacac agtcggtttg ggcggcatct
     6301 actccaccgg cgagcgcgac aaattcatcg tccgtatcga acagggcaaa aacggcgttt
     6361 ccgacatctt cttcgcccac aaagccatga aagaagtgta cggcggcaaa gacaaagaca
     6421 cgaccgtatg gcagccctcc ccgtccgatc ccaacctcga agccgctttc ctgacgcgct
     6481 ttatgcaata tttgggcgtt gacggacagc aggcggaaaa cgcatcggca aaaaaaccta
     6541 cccttcccgc cgccaacgaa atggcgcgta tcgaaggcaa aagcctgatt gtctttggcg
     6601 actacggcag aaactggcgg cgcaccgcgc tcgccctcga ccgcatcggg ctgaccgtcg
     6661 tcggtcaaaa caccgaacgc cacgctttcc tggttcaaaa agccccgaac gaaagcaatg
     6721 cagttaccga acaaaaaccc ggcctgttca aacgcctgct gggcaaaggc aaagcggaga
     6781 aacctgccga acagccggaa ctgattgtct atgccgagcc tgtcgccaac ggctcgcgca
     6841 tcgtcctgct caacaaagac ggcagcgcat atgccggcaa agacgcatcc gcattattgg
     6901 gcaaactcca ttccgaactg cgttaaaccg ttcaaaacaa aagcagcttc caaacagaag
     6961 ctgctttttt gtgcggaact ttttacaaca accgaggaaa aatatcctca accgcctgaa
     7021 ccaacgcatc cgccaaaggc ggatcgaacg cacgatgccc tgcctgaacc accctcaatt
     7081 ccgcttcggg aaaggctttc gacagcgccc acgcactctg catcggcgta cacaaatcat
     7141 accgcccctg cacgataatg gtcgggatat gctgtatttt gccgatattg ttcaaaatcg
     7201 ccctatcgcc ctgcaaccag ccgccgttga caaaataatg gttttccaaa cgcgcgattg
     7261 ccagcgaggc ataagcatct tcatccactt cctccggctc gaaacggatc agatagcttt
     7321 cccaatccgc ccaagccttt gcggcagaca ggcacacttc ttcatcttga tgaaacagca
     7381 acccgtgata cgcctcaatc agccggttcc gccgattttc agcaatcggc gcgacaaatt
     7441 tttgccattg ttccggataa atccggctca caccgcccgc ctcgttcagc cacaccgttt
     7501 cagacggtct gcacaaaaat atcccgcgca acaccaatcc ctttacccgt tcaggatggg
     7561 tttgggcata agccagcgac aaagtgctgc cccacgaacc gccgaacacc agccatttcc
     7621 cgatacccag catttcacgg actttttcaa tatccgccac caaatcccaa gtcgtattgt
     7681 cttccgcaca agcatacggg cgcgaacgtc cgcaaccgcg ctggtcgatg atgacgatgc
     7741 ggaacacatc gggattgaaa aaaccccgac attcaggcga agcccccgcg cccggcccgc
     7801 catgtaaaaa aatgaccggc acaccgtcgg gattacccga ctcttcccaa taaatttgat
     7861 gaatttcaga cacctgcaag tatccgctat gaaaaggctg ttttatttca tacatcatct
     7921 tctcccatat tgcttctgac aatccgaaca tcccgacaca cgacatcccg cacgaaacac
     7981 aagccgcccc gaaaggcaaa tgccgaaaaa tcgcgatata gtgaattaac aaaaatcagg
     8041 acaaggcggc gagccgcaga cagtacggat agtacggaac cgattcactc gtgcttaagc
     8101 accttagaga atcattctct ttgagctaag gcgaggcaac gccgtac
//
LOCUS       DAXHSJ010000090         8079 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000090 DAXHSJ010000000
VERSION     DAXHSJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.090"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(546..2612)
                     /locus_tag="ACFM8U_001363"
     CDS             complement(546..2612)
                     /locus_tag="ACFM8U_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC6334597.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGWANTVEPLTGITERVGRIWGVVSHLNSVTDTPELRAVYNELMPE
                     ITVFFTEIGQDIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQA
                     ELAKLQTEGAQLSAKFSQNVLDATDAFALYFDDAAPLAGIPKDALAMFAAAAQSEGKT
                     GYKIGLQIPHYLAVIQYADNRELREQIYRAYVTRASELSDGGKFDNTANIDRTLANAL
                     QTAKLLGFKNYAELSLATKMADTPEQVLNFLHDLACRAKPYAEKDLAEVKAFARERLN
                     LADPQPWDLGYAGEKLREAKYAFSETEVKKYFPVSKVLAGLFAQIKKLYGIGFTEKTV
                     PIWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAY
                     LVCNFTPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELP
                     SQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDM
                     MIYSEDDEGRLKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWA
                     EVLSADAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHS
                     GFDNAA"
     gene            complement(2700..3572)
                     /locus_tag="ACFM8U_001364"
     CDS             complement(2700..3572)
                     /locus_tag="ACFM8U_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC6334598.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3842..6232
                     /gene="gyrB"
                     /locus_tag="ACFM8U_001365"
     CDS             3842..6232
                     /gene="gyrB"
                     /locus_tag="ACFM8U_001365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC6334599.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIETNEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            6495..7871
                     /locus_tag="ACFM8U_001366"
                     /pseudo
     CDS             6495..7871
                     /locus_tag="ACFM8U_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
BASE COUNT         1852 a         2206 c         2281 g         1740 t
ORIGIN      
        1 cgtcaccttg ccctgatttt tgttaatcca ctataaaatg ccgtctgaag cgttgtttga
       61 ccgtttcaga cggcattttt atcaaattca aaaatattcc gtcattcccg cgaaagcggg
      121 aatctagaac gtaaaatcta aagaaaccgt tttatccgat aagtttccgc accgacagac
      181 cttgattccc gcctgcgcgg gaatgacggg atttctgttt ttgatttttt tgtttttggg
      241 gaatgacggg atttgagatt gcgggcattt atcgggtaaa acggaaatta aacgttgcga
      301 aaatttatcc gaaatcacag caacttttcc gcgtcattcc cacgaaagtg ggaatctaga
      361 acgtaaaatc taaagaaacc gttttatccg ataagtttcc gcaccgacag acctggattc
      421 ccgcctgcgc gggaatgacg ggattttggc ttactgtttt tgtgaaaatg gcgaaaccgc
      481 gcaaatgctg atgccgtctg aaacactgtt tgattgtttc agacggcatt ttttgttcac
      541 atcgatcaag ccgcgttgtc gaagccgctg tggcgcaaga gtgcgtctat gctcggttcg
      601 cgtccgcgga aggctttgaa ggactctgcc gcgctgcggg agccgccgac ggcgaggatt
      661 tcctgccaaa agcgtttgcc tgtggcggcg acatcgtcgc tttcttcaaa ggcggcgtat
      721 gcgtccgcgc tcaatacttc cgcccacgcg tagctgtaat agcctgcgga atagccgcct
      781 gcgaagatgt ggccgaagct caaggcgaag cggttgtatt cgggcggctg gatgacggcg
      841 acttttttgc gcacgctgtc taaaacctgt tgccagtttt tcagacggcc ttcgtcgtct
      901 tcgctgtaaa tcatcatatc aaagagggcg aactccattt ggcggacgag gaacatgccg
      961 cgttggaagt ttttggcggc gagcattttg tcgaagagtt ctttcggcag gggaacgccg
     1021 gtttcttcgt gggcggacat ttgcgccaag acattgtatt cccaaacgaa attttccata
     1081 aactggctgg gcagttcgac tgcgtcccat tctacgccgt tgatgccgga tacgcccagt
     1141 tcgtcgactt gggtaagcag gtggtgcagg ccgtgtccgg tttcgtggaa gagtgtgagg
     1201 atttcgtcgt ggctcaaacg cgcttctttg ccgccgacgg gcggggtgaa gttgcagacg
     1261 aggtaggcgg tgggcagttg cagcgtgccg tcggcgaagc ggcggcggcc tttgtagtcg
     1321 ttcatccatg cgccgccgcg tttgccttcg cgtgcgtaca aatccatata aacgccgcct
     1381 atggtttcgc cgttttgttg caattcaaaa tagcgcacgt ctttgtgcca gatggggacg
     1441 gttttttcgg taaatccgat gccgtaaagt tttttgattt gggcgaacag gcccgccaga
     1501 actttgctga cggggaagta ttttttgact tcggtttcgc tgaatgcgta tttggcttcg
     1561 cgcagttttt cgccggcgta gcccaagtcc cacggctgcg ggtcggcgag gttcaggcgt
     1621 tcgcgggcga aggctttgac ttcggcgagg tctttttcgg cgtagggttt ggcgcggcag
     1681 gcgaggtcgt gcaggaagtt taaaacttgt tcgggggtgt ccgccatttt ggttgccagc
     1741 gacaattcgg cgtagttttt gaagccgagc agtttggcgg tttgcagggc gtttgcgagc
     1801 gtgcggtcga tgttggcggt gttgtcgaat ttgccgccgt ctgaaagttc gctggcgcgg
     1861 gtaacgtagg cgcggtagat ttgttcgcgc agttcacggt tgtcggcgta ttggatgacg
     1921 gcgaggtagt gcggaatctg caaaccgatt ttgtagcctg ttttgccttc gctttgcgcg
     1981 gcggcggcaa acatggcgag cgcgtctttg ggaatgccgg caagcggtgc ggcatcgtcg
     2041 aagtagaggg cgaaggcgtc ggttgcgtct aagacgtttt gggagaattt ggcggaaagt
     2101 tgcgcgcctt cggtttgcag ttttgccagt tctgcctgct gttcgggcgg cagttccgcg
     2161 ccgctaagga cgaaatcgcg caggtcgtgg ttgagcttgg ttttttgtgc gggggagagg
     2221 gtgtcgaatt cgggggaatt tttgatggtt ttgaagcggt tgtacagctc gatgtcttgt
     2281 ccgatttcgg tgaagaagac ggtgatttcg ggcatcagtt cgttatagac ggcgcgcagt
     2341 tcgggcgtat cggtgacgga gttgaggtgc gacaccacgc cccaaatcct gccgacgcgt
     2401 tcggtgatgc cggtcagggg ttcgacagtg tttgcccagc cggtcagggg ttcgacagtg
     2461 tttgcccagc cggtgtgcgt ttgggctttg atggcggcga tttgttcgcg cgcttcggca
     2521 atggcggttt gcagggcggg tttgatgtct tcggttttga tttgatcaaa acggggttct
     2581 tcgcccaaat ggagcagtgc gttgtcagtc ataagatggg tttcctttgc gtgttgtgct
     2641 tcagacagca tatcggcaat atgccgtctg aagccggaaa tggggtcggt cagggaaagt
     2701 taaagtgttt cggagccggc ggggtaattt aacaggcggt attgtacgcg caaaaattcg
     2761 tccataaccg cctgttggat ttccagccgc tttgaaacgg gggaggcgaa gcggacgatg
     2821 atgcggtatg ccttgtcatc gtacggcacg cgggtaacgc gcggtctggc ggcgggcgtg
     2881 ataaacagtt tttccgcctg cacgttttcc aaatgccgtt ggatggcggg gatgtagggc
     2941 gcgcacaagg gctcgagtac ggctttcaga cggcatacgg cttcatccga atccaaatgg
     3001 atgggaacgg ggatttcgac cgtatggatg acatagtcgc ccaaaatatt gtcgcggcgc
     3061 acggggtggc tcaacaacag gctgttgggg aaagaaacgg tggttcccgc aagctgtccg
     3121 accaaggggt tcggaccgac ctgcatcatc agcgtgttca acaggttgat gtcgaccacg
     3181 cgcccgcgca ggccgttgat ttcgatatag tcgccgaccg agtattgctg ggtggcagac
     3241 cttaaaatac tgcccgacag acacataatc agttccttcg tcgccacgac gaccgccgcc
     3301 gccaccgcaa acatcgacaa agccagcgtt tggatttgcg ccgaccagat aaatgccagc
     3361 gaaaacagca ccaaaagcag cgttatattg cggctggcaa ccaaaaaccg ccgcttgctt
     3421 tcgatgccga aatccggatg ccgtttgaag tggatattca acagaagggc gcgcgccagc
     3481 agcaaagccg caaccgccgc cacggattcg accgcctccg cacgtatcgg gacggcaccg
     3541 agccaagtgt ccaacatatt ccatatttcc atttccgcgc ccctgtccga aaagtatagg
     3601 gtgtagattt tagtggcaaa aaaacgcttt tgccacttta cgggaaaatc cccgacctga
     3661 tgccggaggc aaacgtaccg tttcatcttt tcccggcggc acactgatgc ggataacgct
     3721 gaattaggcg gcaaaatcgg ctataataca caattatata tgccgtcccg cccgcttttt
     3781 ccacaaggtg cggacttgtt ttggcggcat cgcaaaatct tttcaaaatc cggcaaaaaa
     3841 tatgactgaa caaaaacacg aagaatacgg cgccgacagc attcaggtgc tcgaaggctt
     3901 ggaagcggta cgcaaacgcc ccggcatgta catcggcgac acgcaggacg gcagcggtct
     3961 gcaccatatg gtgtttgaag tattggacaa cgccattgac gaagcactcg ccggacattg
     4021 cgacaaaatc acggtaacga tacacgccga ccattccgtc agcgtcgccg acaacgggcg
     4081 cggtatgccc accggcatcc acccgaaaga aggacgctcc gccgccgaag tcatcatgac
     4141 cgtattgcac gcgggcggta aattcgacaa caacagctac aaaatctccg gcggcctgca
     4201 cggcgtgggc gtgtccgtcg tcaacgcgct gtccgactgg gtaacgctga ccatctaccg
     4261 cgacggcaaa gaacacttcg tccgcttcgt gcgcggcgaa accgaagagc cgctgaaggt
     4321 tgtcggcgat tccgataaaa aaggcacgac cgtgcgcttc ctcgccagta cggaaacctt
     4381 cggcaacgtc gaatacagct tcgacatcct tgccaaacgc atccgcgaac tttcgttcct
     4441 gaacaacggc gtggacatcg aattgaccga cgagcgcgac ggcaaacacg aaagcttcgc
     4501 cctttccggc ggcgtggctg gattcgtgca atacatgaac cgcaaaaaaa cgcccttgca
     4561 cgaaaaaatc ttctacgcgt tcggcgagaa agacggcatg agcgtcgaat gcgcgatgca
     4621 atggaacgac agctatcagg aaagcgtgca gtgcttcacc aacaacatcc ctcagcgcga
     4681 cggcggtacg cacctgaccg cgttgcgcca agtgatgacg cgcaccatca acagctacat
     4741 cgaaaccaac gaagtcgcca aaaaagccaa agtggaaacc gccggcgacg atatgcgcga
     4801 aggtttgacc tgcgtgttgt ccgtcaaact gcccgacccc aaattttcgt cccaaaccaa
     4861 agacaaactg gtttccggcg aaatcggtcc cgtcgtcaac gaagtcatca gccaagccct
     4921 gaccgacttc ctcgaagaaa accctgccga agccaaaatc atcaccggca aaatcgtcga
     4981 tgccgcccgc gcgcgcgaag ccgcccgcaa agcccgcgaa atcacccgcc gcaaaggcgt
     5041 gatggacggc ttggggctac ccggcaaact cgccgactgc caagaaaaag accccgcatt
     5101 gtcagaactt tatctggtcg agggcgattc tgcaggcggt tccgccatgc aggggcgcga
     5161 ccgcaaattc caagcgattt tgccgcttaa gggcaagatt ttgaacgtcg aaaaagcgcg
     5221 ctttgaaaaa atgctcgcca gtcaggaagt agccgctttg attaccgcgt tgggcgcggg
     5281 catcggcaag gaagaattca atgccgagaa gctgcgttac caccgcatca ttatcatgac
     5341 cgatgccgat gtggacggcg cgcatatccg caccttgctt ttgaccttct tctaccgcca
     5401 aatgcccgaa ctggtcgagc gcggctacat ctatatcgcc cagccgcctt tgtataaagc
     5461 gaaatacggc aaacaggaac gttacctcaa ggacgagttg gaaaaagacc aatggctgct
     5521 cggtcttgcc ttggaaaaag ccaaaatcat ttcagacggc cgcaccatcg aaggcgcaga
     5581 acttgccgac accgccaaac aattcctgtt ggcaaaaacc gtcatcgaac aggaaagccg
     5641 cttcgtagac gaactcgtcc tgcgcgccat gctacacgcg tcgcccattg atttgacgtc
     5701 gtctgaaaac gccgataaag ccgttgccga actttccggt ctgcttgacg aaaaagaagt
     5761 cgccctcgaa cgcatcgaag gtcatgaagg acaccggttc atcaaaatca cgcgcaagct
     5821 gcacggcaac gtcatggtca gctacatcga acccaagttc ctcaacagca aagcctacca
     5881 aaccctcacc caaaccgccg ccgcgctcaa aggcatggtc ggcgagggtg ccaagcttta
     5941 caaaggcgaa aacgggtacg acgcggacag ctttgaaacc gctttggaca tcttgatgag
     6001 cgttgcccaa aaaggtatgt ccatccaacg atacaaaggc ttgggcgaga tgaaccccga
     6061 gcagctgtgg gaaaccacga tggatcccgc cgtgcgccgc ctgttgaaag tgcgcatcga
     6121 agatgccatt gccgccgacg aagtgttcgt tacgctgatg ggcgacgagg tcgagccgcg
     6181 ccgtgccttt atcgaaaaca acgcgctgat tgcccaaaat atcgacgcat aagtgccgtt
     6241 ttaaaaaagg agacgggcat cgtgccgcgt ctcctttttg gttggtcaaa cggaagccat
     6301 accgtctgaa accgcctttc ggagcaaaac catggtcagc atttttgata tagtggatta
     6361 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg aaccgattca
     6421 cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgctgta
     6481 ctggtttaaa tttaatccac tatattttca aaatcggtat cgggccttcc agttcgcata
     6541 cggtcggccc gatgaaggca gccgccgcct ttgcggcagg tttggatgca caggctgttc
     6601 gcatcgtcat cgacatttac ggctcgctcg cactgaccgg atacggacac ggtacatttg
     6661 acgcgctgat gctcggtttg gaaggcagcc tgccgcacga catcccgctt gccggcattc
     6721 ccgaacgcct cgaacgcatc cgcacgcagc acatcctccg gctcaacggg caagaaatcc
     6781 gcttcatccc cgaccgcgac ctgaacatac tcggcaatca agtgctgccc aaacacccca
     6841 acggcctgcg ttttaccgcc tatgcttcag acggcacggt attgaatgaa caggtttatt
     6901 attcggtcgg cggcggcttt gtcgttaccg aagaagattt tgaccggcag gcggaaacgg
     6961 aaaaagccgt tccctatccc tataccagtt gcgccgaact gcttgcccga tgccgtctga
     7021 accggctcga catctccgaa gtcgtgttgg caaacgaagc cgcgcttgcc ggatgcggcg
     7081 aagccgaaat ccgccgccgc gccgctgccg ttgccgaggt tatggaaggc tgcatcaaac
     7141 gcggcttggg tgcggacggc gaactgccca gctgattgaa cgtccgccgc cgcgccccgc
     7201 agcttgccgc caagctcaaa gtcctgcgcg aaaccgaaat cgtcaacacc cagctctggc
     7261 cgatggtgta cgccatggcg gtcaacgaag aaaacgccgc cgacggacgc gtcgttaccg
     7321 caccgaccaa cggcgcggca ggcatcattc ccgccgtatt gcactatttc cgcaagttca
     7381 atccgcacgc cacacaggaa cgcgtcgaaa acttcctgct caccgcaggc gcaatcggca
     7441 tcctctacaa gaccaacgcc tccatttccg gtgcggatgt cggctgtcag ggcgaagtcg
     7501 gcgtagcgtg ttcgatggcg gcgggcgcat acgccgaagt catcggcggc acgcccaaac
     7561 aagtggaaaa cgccgctgaa atggcgatgg aacaccattt ggggctgact tgcgaccccg
     7621 tcggcgggct ggtgcaaatc ccctgtatcg aacgcaacgg catcgccgcc gaaaaagccc
     7681 tcaaactcgg cacgctcgcg cttttggaag acggcacgga caaaaaagtc tcgctcgacg
     7741 aagtcatccg aaccatgctg caaaccggcc gggatatgaa ggcgacctac aaagaaacct
     7801 cgcttgccgg actcgccgcc acgctccgga aaaaagccgt ccccgtatcc gtgcgcgtgg
     7861 tcgagtgctg aggatggaca gaaacgaaaa tgccgtctga aaacagtttt ttagacggca
     7921 tttcatttat ttcttgcggc tttaccgttt cagtgccagc gtcaacacgc ccgccgtaac
     7981 cagccccaag cctatccatt cctgcgtgtt cgggcgttcg tctaagaaaa ccaccgccat
     8041 caggaatgca aaccggcgtt gtttgcagca tccggcaag
//
LOCUS       DAXHSJ010000091         8076 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000091 DAXHSJ010000000
VERSION     DAXHSJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.091"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            96..404
                     /locus_tag="ACFM8U_001367"
     CDS             96..404
                     /locus_tag="ACFM8U_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334600.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            486..1517
                     /gene="ruvB"
                     /locus_tag="ACFM8U_001368"
     CDS             486..1517
                     /gene="ruvB"
                     /locus_tag="ACFM8U_001368"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC6334601.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHTLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1584..2267)
                     /gene="rpe"
                     /locus_tag="ACFM8U_001369"
     CDS             complement(1584..2267)
                     /gene="rpe"
                     /locus_tag="ACFM8U_001369"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC6334602.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPQTLEKIRQV
                     RAMLDCYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RVELAKAAG"
     gene            2413..2847
                     /locus_tag="ACFM8U_001370"
     CDS             2413..2847
                     /locus_tag="ACFM8U_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC6334603.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(2860..3723)
                     /locus_tag="ACFM8U_001371"
     CDS             complement(2860..3723)
                     /locus_tag="ACFM8U_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6334604.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(3731..4348)
                     /locus_tag="ACFM8U_001372"
     CDS             complement(3731..4348)
                     /locus_tag="ACFM8U_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC6334605.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(4511..5125)
                     /locus_tag="ACFM8U_001373"
     CDS             complement(4511..5125)
                     /locus_tag="ACFM8U_001373"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC6334606.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRIGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            5331..7103
                     /locus_tag="ACFM8U_001374"
     CDS             5331..7103
                     /locus_tag="ACFM8U_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HFC6334607.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGESDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFSDGISLTGDDRTLLQTLIRQLGVSLASAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            7100..7756
                     /locus_tag="ACFM8U_001375"
     CDS             7100..7756
                     /locus_tag="ACFM8U_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6334608.1"
                     /translation="MTIKIILIDDHTLFRSGIKSLLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1988 a         2367 c         1888 g         1833 t
ORIGIN      
        1 agttttgagg aggataccca atccaagctt tgcaacagcc gccgccatat ccgctataat
       61 tcacgcttca gccatcctgc ccccgacata aaatcatgac cctgaaaacc gatttattgc
      121 ctaaaatcaa caacgaagat tatcaacgcc tcatcctcaa acacagtgcg gaatttagcg
      181 gtggcgaaat ccgcctgttg aacgaaatcc tcgaaaaatt caatttcgac gttgttcagg
      241 cgcaggcatt ggcgcaggcg gtgatgcagc aagtccgctt cgaccccaac gcctatcaca
      301 tcgacagcga cgacgaagac accacaggca tctgcccaca ctgcatcaac ccgcctatgc
      361 cgcccctgcg cgactatctc gtttggcgcg aaacgcgcgg gtaaaacgct tttgaccgtt
      421 atcttttcaa tgccgtctga aacgccgccg accgttcaga cggcataccc gacaaaggga
      481 acactatgct gcaaaccgac aacctgaccg ccgcgcaacc gcaacgcatc gttgccgccc
      541 aaaccgcctc cgcacaggaa gaactgctcg aacgcgccct gcgtcctaaa acgctggacg
      601 actatatcgg gcaagacaaa gccaaagaac agcttgccat cttcatccaa gccgccaaaa
      661 aacgcggcga agccctagac cacaccctgc tcttcggccc gcctggactg ggcaaaacca
      721 cactggcgca catcatcgcc aaagagctgg gcgtcaacct gcgccaaacc agcggccccg
      781 tcctcgaacg cgcaggcgac ctcgccgccc ttttgaccaa ccttgatccg cacgatgtat
      841 tgttcatcga cgaaatccac cgcctcagcc ctgttgtcga agaaatcctc tatcccgcgc
      901 tcgaagacta ccggctcgac attatgatag gcgaagggcc tgccgcccgt tccgtcaaaa
      961 tcgacctgcc gcccttcacc ctcgtcggcg cgaccacccg cgccggtatg ctgaccaatc
     1021 cgttgcgcga ccgcttcggc atcgtctccc gccttgagtt ttacgaaaac cgagacctta
     1081 ccaccatcgt cagccgttcg gcacaactgt tgcagctcga tatgtccgaa gaaggcgcgg
     1141 aagaaatcgc caaacgcagc cgcggtacgc cgcgcatcgc caaccgcctg ttgcgacgcg
     1201 tgcgcgattt cgccgacgtg aaaaacaacg gcacaatcga cggcggcatc gccgatgccg
     1261 ctttaagcat gctggacgtg gacgcgcagg ggctggacgt gatggacagg aaatttctcg
     1321 aagccgtttt gcacaaattc ggcggcggcc cggtcggttt ggacaatgtt gccgccgcca
     1381 tcggcgaatc aacagacacc atcgaagacg ttatcgaacc ctaccttatc caacaaggct
     1441 tcctgcaacg caccccgcgc ggcaggatgg cgaccgaacg cgcctacctg catttcgggc
     1501 tgcccgtcga aaaataacgc aatgccgtct gaaacagagc taattttcag acggcatttc
     1561 tatttcaatc attggcgcaa ggttcagcct gccgcttttg ccaattcaac cctcatcgca
     1621 tcaatcaccg ccttatagtc tggtttgccg aaaatcgcag aacctgccac aaaggtatcc
     1681 gcgccggctt gggcggcggc ggcaatattg tcggttttga tgccgccgtc cacttcaatg
     1741 gcaatacgtc tgccgctttt ctcttcatag cagtcaagca tggcgcgaac ctgtctgatt
     1801 ttttccaaag tctgcggaat gaagctctga ccgccgaacc cggggttgac cgacatcagc
     1861 aaaaccatat ccagcctgtc caatacgttt tccaacagat atacgggcgt tgccggattc
     1921 aacaccagcc ccgcctgaca gcccatatca cgaatcaggc tcaagctgcg gtcgatatgg
     1981 cggctcgctt cgggatgaaa agtgataatc gatgctcctg ctttggcaaa cgactgaatc
     2041 aggtcgtcaa cgggttcgac catcaaatgc acatcgatcg gcacgcttgc ataaggcttc
     2101 aacgccgcgc aaaccatagg gccgaaggtc aggttcggca cataatggtt gtccatcacg
     2161 tcaaaatgga tcagatctgc acctgccgca atgacgcttt ccacctcttc tccgaggcgg
     2221 gcaaagtctg ccgacaaaat actgggtgcg atacggtagg cggtcatatt ttttccttca
     2281 atgcctttac gggcggactc aacgcgggaa agccccgaaa cagcattatt aacaaaagtt
     2341 aactgccata ataccatctt tcacacggca atcaagtata ttgtccgaaa tatctggatt
     2401 aaaggaaaag ccgtgccact gcctgctccc tgccgttttg ccaaacctgc cgcctctttt
     2461 ttaagtatgg ctttgctttc ctgtcagctt tcccacgccg ccacggctta tatccccccg
     2521 aacgattttc aaccgaactg cgacatacgc cgactcgggc tgacccaaag tcagcacaat
     2581 gagctgcgta aaatccgcac cgccttcaaa atggcgggcg acagggcgcg tttgaaggtt
     2641 atgcattccg aacacagccg ccgccggtct gtcgtcgaaa tcatttcctc ggatgttttt
     2701 aatcggaacg aggcgcgcga ttatgtcgaa agccgctatt tgtccggtat ggattttgcg
     2761 gtggacgaat tggaaatcca acaccggttc ttccatatcc tcacaccgca acagcagcaa
     2821 atgtggcttt cttcctgcct caaataatcc ccgaaacgct cacaacgccc gttgtttcgg
     2881 cagcctgccc gcccagtcgc aggcaaactg ccacgcggaa cgtcccgaac ggctgccccg
     2941 cgtctgcgcc cactgcaatg ccgccatctg tgcggtttca tcatacggca cgccgaaatc
     3001 ttccagccaa ttttgcaccg ccgccagata atcgttttga tcgaacggat aaaaactgag
     3061 ccacaatccg aatcggtcgg acagggatat tttttcttcc accgcttctt tctgatggat
     3121 ttccccccgc atccccgtcg taccggcatt ctcgtcaaaa tattcgggca tcaggtgccg
     3181 tctgttggaa gtcgcgtaaa ccaaaacgtt ggcgcaacgt tgagacagac cgccgtctaa
     3241 cgcggttttc aatgccttat aggtttcatc gccgctttca aacgacaaat cgtcgcaaaa
     3301 tacgataaat ttttccggac gttccttcaa aagcgtcaac aggtaaggca ggccgattaa
     3361 atcgctttta tcgacttcga tcaggcgcaa tcccttatcc gcatattcgt gtagcagggc
     3421 tttgaccagc gaggatttgc ctgttccgcg cgcgccgctc atcaatacat tgttcgcggg
     3481 tctgccgaca atgaactgtt cggtattacg caccagcaat tcggtttgcc tgccgactcc
     3541 cgccagcctt accaagggaa aggtgtgcgg atcgggcaag tgttccaaaa aacctttttt
     3601 gcccgcactc tgccagcgga aggcaagcgc gttccaatcc gtatgcccgg gttcgggcgg
     3661 aagcacggca tccaaacgcc gcaaaacggc ataggcttta tcgagaaatt cgttcaattc
     3721 catctctgcc tcactttgca tatctttgcg ccatcagccg ttcgacggta tcgacgattg
     3781 cctgcgtatt cggatcgatt tcgatgttga tcctgtcgcc gacctttctg ctgccgaaca
     3841 gcgtccgttc caaagtttcg ggaatcagat ggacattgaa acggctgtct tcgactttgc
     3901 ctatggtcag gctgcaaccg tccaagccga cgaacccttt ggtcaggata tagggtttga
     3961 gttcgtgcgg gagcgaaaac caaaccgtgc ggttgtaccc gtcccgttcg atttcgacaa
     4021 taggcacggt tgccataatg tgtccgctca tgacgtgtcc gccgatttcg tctccgaaac
     4081 gcgccgcccg ttcgatgttg acgcaatcgc cttccttcag cagccctaaa ttggtttttg
     4141 ccaaagtttc cgccattaaa tcgaaactga cgcggtttcc ttcgatttcg gtaatcgtca
     4201 ggcagcagcc gttgttggcg accgatgcgc cgcgttgcag attgtccgcc gcctcttgcg
     4261 gaagctcgac gacataagtc tgaaatgcct ccgacgggcg gtggattgcc gtcagttttc
     4321 ccaatccttg aacaatgcct gtaaacataa tcctgtttcc ctgtgtcggt aaaaatggtg
     4381 caaattgtag catctccccg cgaaaaatgc cgtctgaaat gccttcagac agcattatgc
     4441 ctccgattcg ggcaaaaacc gcccggtacg gcttgacctt tcctttccac gccggtcggc
     4501 ggtcttaccc ttatccctcc tgcaaatcag tttgcgtgtt caagtcggca aaatgcccgt
     4561 caaactcgaa tctgaccggt cgcgcccttt gctgctgcaa ccagcttctt aatgttttca
     4621 tacccgaaaa cagataggga atcgcgcttt gcagaatctg cgggcggata tacataatgt
     4681 tgtagtgcat cgttatcggc gtttccacat aatacgcatt gcacaacggt gtgcgtttcg
     4741 acacggtttc aaacctcgcc accaaatcgt ccggcagata cggcatatcg cacggcacaa
     4801 ccaaaagcca gtcggcagtc gccaactgca aatcgttggc tgcggtacac aatgccgaaa
     4861 gcgggccgaa atgctgccac tgccgtgcgt ccggaaaaat atgcggactt cttcgagcat
     4921 attcttccaa attccggttg gtgctgatgg cgatatggct gacctgcggc ctgaccctgt
     4981 cgatgacatg atctatcagt gccttacccc ccaaaagagc aagccccttg tcctcgcctc
     5041 ctatacggct cgcctgaccg tcggccagta tcagggcaaa agttttcatt gcggatgttc
     5101 tcttggaaaa gttcgaggtt ttcatgattg cagtctgccg ttcccaatga ctcaaaatgc
     5161 cgtctgaagc agacggcaaa taaattcata ttatctgaat tttatcataa catgatttaa
     5221 cactgaagcg gtgcggatta agttttcctt aaccaattct ttctgagcag ttgtatctaa
     5281 ttccaaagaa tgatattgtt tgcattattt ggaacaattt ttcgccgagc atgatactgc
     5341 cagcccgttt ttcagacggc atcagccttt ccctacgcct gaaactcctg accggagtgt
     5401 gggtcgggtt ggcggcattg tctgtcgttt tgacactgct gctctctttg cgtctggaaa
     5461 acgcggcctc cgtcatcgaa gaggcgggca acttgagaat gcaggcatac cgtctggcat
     5521 acatggcggg tgaaggatct ccccgctccc aaatagacaa tcaggttgac gaatttgaaa
     5581 aaagcctgaa tcgtatttca caaagtgatg ccatccatcc gctgattcct tcggacaccc
     5641 ctcttgctta tgatttgata caatccatgc tgattataga ttggcaggca cacatcctcc
     5701 ccccgctcca gtcctaccgg cgaccgactc aggtcgatct ctaccgcttt gccggaaaca
     5761 tcgaactgtt tttgcaggca ttggaaaatg ccaacgaaaa aaacacatgg tggctcaggc
     5821 gttttcaatg ggcaattatg ttgatgacgc tggtgtcgtc tgtactgatg ctgttttggc
     5881 accagatttg ggttatccgg ccgctgcagg cgttaaggga aggtgcggaa cgcatcggac
     5941 ggaggtgttt cgatattccg gttcccgaag gcggtacgcc ggaattcaaa caggtcgggc
     6001 gttgtttcaa tcaaatgggc ggcaggttga aaattttata tgatgatttg gaaggacaag
     6061 tcgccgagca gacacgcagt ctcgaaaaac aaaatcaaaa cctgaccctg ctgtaccaaa
     6121 ctacacggga cctgcaccaa tcctacatac cgcaacaggc tgcagaacat tttctaaacc
     6181 gtatcctgcc cgccgtagga gcagattccg gcagagtttg tttggacggc gaatccgatg
     6241 tttatgtttc cattcatcat gcggattgcg gcacagcagc ttcggatttg gggaagtacc
     6301 atgaggaaat cttccccatt gagtaccaga acgaaacatt gggcaggctg ttgctcagct
     6361 tttcagacgg catttccctc accggagacg accgcacgct gcttcaaacc ctgattcggc
     6421 aattgggcgt atcgctcgct agcgcgaagc aggaagaaga aaaacgcctg cttgcagtat
     6481 tgcaggaacg caacctgatt gcgcaaggat tacatgacag catcgcacaa gcattaacgt
     6541 tcctaaacct acaggtacag atgctggaaa ccgcctttgc cgaaaacaaa cgggaggaag
     6601 ccgcagaaaa catcggcttc atcaaaacag gcgtgcagga atgttatgaa gatgtccgcg
     6661 aactgctgct caacttccgt accaaaatca gcaataaaga atttcccgaa gccgttgccg
     6721 acctattcgc ccgctttacg caacaaaccg ggataacggt cgaaaccgcc tgggaaaacg
     6781 gttcgttcct gccgcctcag gaagcgcagc tccaaatgat ttttatcctg caggaaagcc
     6841 tgtccaacat ccgcaaacac gcccgcgcca cccatgtaaa attcaccctt tccgaacacg
     6901 gcggacgctt taccatgacc atccaagaca acggacaagg tttcgacacg gagaaaatag
     6961 gagaacccac gggcagccat gtcggactgc acatcatgca ggagcgtgcc aaacgcatcc
     7021 atgccgtttt agaaatccgt tcccaagctc aacagggaac caccgtctca ttgacggttg
     7081 catctgaaga aagcttgaaa tgaccattaa aattatcctg atagacgacc ataccctctt
     7141 ccgcagcggc attaaatccc ttttgtcgcg ccaacatgat tttgaggtca tcggcgaagc
     7201 cgcagacggc ctctcgggca ttaaaatgat caaccggctc caacccgatg tcgtcctgct
     7261 tgaccttgat atgcccggta tgaacggacg cgaagcactc tcccaaatca tcagcatcaa
     7321 tccgcagcag gcagtgatta tgctgaccgt ttccgaagac aacgacgact tgaccgaatg
     7381 tatgcgcatc ggcgcgcgcg gctacctgct gaaaaacatc aacgccgact ttctgctcga
     7441 aagcatacgc aaagccgccg aaggcgataa tgtattctcg cccgagatga ccgccaaact
     7501 cgtcaaaagc ctgatttccc cccaacctgc ccaagggacg caggcactct cctcacttac
     7561 ccctcgcgaa ctggaaatct tgggctatct cgccgcagga cacagcaaca aaatcatcgc
     7621 ccgccacctc gatcttgccg aatccaccgt caaagtccac gttcaaaacc tgctccgcaa
     7681 actcaacctc agcagccggg tgcaggccgc tgtttacgcc atccggcaca acgtccccca
     7741 acccgtgctg gaataaggcg ggttcagacg gcatattagg ggttttaatc cccgtacggt
     7801 cattcggata acagaccaag catgtaagtt tatgccccca taagtacgct tggcatagca
     7861 gtaatattgt tcggttcagt gttttccgtt tgcccctatc tgatactgca atatcagcta
     7921 tgccgtctga aaacgcatca tcatgatatt ttcagacggc ataataaaaa gcggaaatac
     7981 taatgcaggg taaaatgttc cattccgaat cccataaata tacaatggct tatatcgttt
     8041 aagcatgtgt aacccaccct catatcaatc aatata
//
LOCUS       DAXHSJ010000092         8058 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000092 DAXHSJ010000000
VERSION     DAXHSJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.092"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(96..320)
                     /locus_tag="ACFM8U_001376"
     CDS             complement(96..320)
                     /locus_tag="ACFM8U_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC6334609.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDTQEPYSLRDEIPPHY"
     gene            complement(321..1709)
                     /locus_tag="ACFM8U_001377"
     CDS             complement(321..1709)
                     /locus_tag="ACFM8U_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC6334610.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLTGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1795..2616)
                     /gene="prmC"
                     /locus_tag="ACFM8U_001378"
     CDS             complement(1795..2616)
                     /gene="prmC"
                     /locus_tag="ACFM8U_001378"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6334611.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRTSDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIASNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(3132..4616)
                     /gene="tldD"
                     /locus_tag="ACFM8U_001379"
     CDS             complement(3132..4616)
                     /gene="tldD"
                     /locus_tag="ACFM8U_001379"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC6334612.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLMGTQSTGNGRRESYASAPMPRMTNTFMENGGYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGKLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      4691..4794
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4860..6083
                     /gene="cytX"
                     /locus_tag="ACFM8U_001380"
     CDS             4860..6083
                     /gene="cytX"
                     /locus_tag="ACFM8U_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC6334613.1"
                     /translation="MSGNASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            6080..7180
                     /locus_tag="ACFM8U_001381"
     CDS             6080..7180
                     /locus_tag="ACFM8U_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC6334614.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNQAPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHIFVVGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            7201..7818
                     /gene="thiE"
                     /locus_tag="ACFM8U_001382"
     CDS             7201..7818
                     /gene="thiE"
                     /locus_tag="ACFM8U_001382"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC6334615.1"
                     /translation="MTFLPLKSPLKFYAVVPTADWVGRMVEAGADTVQLRCKALHGDE
                     LKREIARCAAACRGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAVVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         1738 a         2461 c         2177 g         1682 t
ORIGIN      
        1 tcctgatttt tattaatcca ctatatatct taggtttgca tcggcaaaat attcaaacac
       61 agcctttttt aaggaaatcc ggatacggcg gcacatcaat aatgcggcgg aatctcgtcg
      121 cgcagggaat acggctcttg cgtgtcggga ttcctgtcct gcattttttg atacagcagc
      181 ctcaactgag cctgctgcaa atccagcgtc tgccgcaatt ccgccaccat cgcgttcagg
      241 ccggcgatta cgtcctcctg aagcgcggat tggatttcca gctctgtgat gcggtgttcc
      301 aactcttgaa ccgcgtccat ttacagcacc atcgcggcaa tccagccggc aatcagcagc
      361 gggatattgt agtggatgaa ggtcgggata acggaatcgc ggatgtggtc gtgctgcccg
      421 tcggcgttca gccccatcgt cgggcccagc gtggaatcgg acgcaggcga accggcatcg
      481 cccaacgccc ccgccgtgcc gacaatggcg acggtggcaa gcggcgaaaa acccaaaccg
      541 acacacaaag gcacataaat cgcggcaata atcggcaaag tggaaaagga cgaaccgatg
      601 cccatcgtta ccaaaagccc caccaccagc atcgccaatg ccgccatacc tttgctgttg
      661 ccgaatatcg ccatactgct ttccaccagc ggctggatat gcccggtcgc attcatcacg
      721 gcggcaaaac cctgcgcggc aatcataatg aagccgacca tcgccatcat cttgatacct
      781 tcgccgaata cgtcgtttgc cttgtcgcgg ttgatgaccc ccaacatcat aaatacggcg
      841 aaaccgagca tcgcgcccaa caccagcgag tcttcataca tcaactggat ggcaaaacat
      901 acggcaatgg cggcggcggc ggccaggctg cggtaggcgg acggctgcgg acggtttgcc
      961 gcatcggcgt tgcccgccgt atcggcattg ttgctttggt acaggcgcgg tttgcggtaa
     1021 tggacaaacg ccagcaggag tccggtcagc attcccaacg cgggaatcgc cattgccgcc
     1081 attacgttga tgtttttcac atcaagctgc ggcgcggcgg aatggatgtt gcccaacagg
     1141 atttcgttca aaaaaatcgc gccgaagccg taaggcagga acatataggt cgtaaccagc
     1201 ccgaaagtaa tgacgcacgc aatcagacgg cggtcgattt tcaggcggtt gaacaccaaa
     1261 agcagcggcg gaacaatcat cgggataaag gcgatgtgga taggtatgat gttctgactc
     1321 ataatgccca tcacaaggat gatggaaagc agcagccatt tgaccgcgcc ctcgcccgaa
     1381 cgcacgctgt cgggcatacc gccccggttc agcttgcgga cgaccgcgcc ggcaagctgc
     1441 tgcggcaggc cggaatgggt aatcgccatt gcaaacgcgc cgagcatcgc ataagaaagc
     1501 gcaatcttcg caccgccttc caaacctttg ttgaacacgg ggataatccc cgcctgactg
     1561 acctgtcccg ccgcatcggc aatgttttgc agcggcatac ccgccaccgc gccgccgaca
     1621 aacgcgccga ccgtcaggct caataccacg tgcacgcgcg acagcgacag caccagcata
     1681 acgattacgg caactacgac tgcattcatt gtttatcgct ccaaacctat aaatgtttac
     1741 atatcgaaac acatcataac ccaataatgg aaaacccgcc aattttgcaa acaattattt
     1801 caaatgcttc atatacttcc ccagcgtaac cctgtccaaa cccgccaagt ccggcagggt
     1861 ttccactcct gaaaaaccat tctccgccaa cacgccgcgc accgccgcgc cctgatcgaa
     1921 accgtgttcc aacaacaaaa aaccgccttc cgccaaacgg tcgggcgcgc cttgcgccaa
     1981 ggtgcggatg cagcttaggc cgtctgaaaa gtcggtcagc gcgatttgcg gctcaaaccg
     2041 caaatcgcct tgcaacaaat gtttatcgcc gttttcgata tagggcgggt tggacgcgat
     2101 aatgtcccat ttcccttcag acggcatatc ggtgtcgaac cacgaaccgt gtgcaaattc
     2161 gacccgcgcg cccaaatccg ccgcattttt ccgcgccgtt tcaagggcgg gcgtgctgat
     2221 gtcggatgtg cgcacaaacg catcggggcg ttcgagtgcg acggttacgg caaccgcgcc
     2281 gctgcccgtc cccaaatccc acacgcgccc gttttcgggc aggcgcgcca atacggcttc
     2341 gaccaaatgt tcggtttcgg ggcgcggaat cagcacgctc ggattgactg taaagcgtct
     2401 gccataaaat tcgcgcacac ctaaaatata ggcaaccggc tcgccgttca gacggcgttg
     2461 cgccagcctg tccgcccgct gtcggacttc gtccggcatt tcttccccgc cccgcgtcaa
     2521 caactgcacg cgcgtatatt ccgaaacata ttgcagcagc attcttgctt catttttagg
     2581 cagttttgac aagcccaacc atttatcaaa cgtcattttt atcccgtctg ccgctgatgc
     2641 ggcttttctt tccttattct ttccggcaaa cgtaccgatg gtggcaaccg caaatgcggc
     2701 ataccacaaa taaaatcctg caccgtagcg cacaatatcc gatgtattcc ctgcttcatc
     2761 gacgtatacg gctttcacac tgaaagccac caacgccaag ccccaaagtg ccgcatggac
     2821 aggcacgacc ttcttccgca acgccagcaa aacaatggcc gccaaccaaa cataattcgc
     2881 atagaccgca caatacctga tatccaaaga agcaaatatc gaccccaaaa tcaaaacggt
     2941 caaaccctcc atgcttccat gattgcccaa ataaaatgca acattggata aagacgctat
     3001 ccacagggca accgacacca gcaacatcac tatgggaaaa cttgatttcc gattctgttc
     3061 ctgcatggtt ttatcctaat gtaaaaggcc gtctgaaaaa actttcagac ggcatcgtgt
     3121 cggattcagc gtcagattgc gctgccgccg acggtcagcc cggcatcgat acgcaaagtc
     3181 ggctgcccca cgccgacggg gacgctctgc ccttctttgc cgcacacgcc gacaccgctg
     3241 tccaacgcgg tatcgttgcc tatcatggaa acatgtttca gcacttcggg gccgttgccg
     3301 atgatggtcg cgcctttgac gggatattgc agtttgccgc cttcgaccca ccacgcttcg
     3361 gatgcactga acacaaactt gccgctggtg atgtccactt gtccgccgcc gaagttgacg
     3421 gcgtaaatgc ccttgtcgat ggacgcaatg atttcttccg gctcatagcc gccgttttcc
     3481 ataaaggtat tggtcatgcg tggcataggg gcggaagcgt aactttcgcg gcggccgttg
     3541 ccggtcgatt gcgtacccat cagacgggcg ttggtttcgt cctgcatata gccgactaaa
     3601 atgccgtctt caatcaatac ggtgcggcgg gtttcgttgc cttcgtcgtc gatgttgagc
     3661 gaaccgcgcc ggccggcaat atcgccctga tcgacgacgg taacgccttt ggcggcgacg
     3721 cgctcgccta ttctgccgga aaagacgctg gttcccttgc ggttgaaatc gccttccaaa
     3781 ccgtgtccga ccgcttcgtg cagcaacacg cccggccagc cgttgcccaa aacgacggtc
     3841 atttcgccgg cgggcgcggg gcgggattcg aggttggtga gtgcctgttt gacggcggca
     3901 tcgacaaact gccgcaccaa gttttcatcg aaataagcca agtcgtagcg tccgccgccg
     3961 cccgcgctgc cctgttcgcg gcgttcgccc tgtttggcga taacggtaac gttcaggcgc
     4021 accatcgggc ggatgtcggc ggcgtgtttg ccgtccagac gggcgaggta aaccatatcg
     4081 tattcgcagg tcaaaccggc catcacttgc acgatgcgcg gatcggcggc tttggcgatt
     4141 gcttccactt tgttcaacag cgcgactttg gcggcggaat cgaggccggc aatggggtcg
     4201 gacgcggaac aaaccggctt gccgcgcgtt tcagacggca ttttggcgga caccttgccg
     4261 cctgccgccc caatcgcgcg gacggcgcgg gcggaacggt ttatcgaatc gatgcacagg
     4321 ctgtcggcgt aggcaaaggc ggttttgtcg cccgaaacgg cgcgcacgcc cacgccctga
     4381 ttgatttgga agctgcccga tttgacgatg ccctcttcca aatgccagct ttcataagcg
     4441 gtgcgctggc agtagatgtc ggcgtaatcg acgtggtgcg cgccgatgat gcacaggctt
     4501 ttggcgagca gttcggggga aaggcggttg gcttcgagca gccgcgcctg tacggcggaa
     4561 taggtcggat gcatagtgtc ggcgcataaa aaatcagggg cttgattata cggcatttgt
     4621 tatggaaatg cgctgtgccg ccgaaaatgt aagaattttg ccaacgcacg ttgctttgtg
     4681 taaacttaaa gctttcttgt cggagtgccg ccgccgggcg gctgagattg cgaaagcaga
     4741 atccgtagaa cctgtcgggg taatgcctgc gtaggaaaca aaacctcccc gccctttcgg
     4801 gcttccgttc ccttttccgc acttccccgc cccattttca tgttttttaa ggacttgata
     4861 tgtcgggcaa tgcctcctct ccttcatctt cctccgccat cgggctgatt tggttcggcg
     4921 cggcggtatc gattgccgaa atcagcacgg gtacgctgct tgcgcctttg ggctggcagc
     4981 gcggtctggc ggctctactt ttgggtcatg ccgtcggcgg cgcgctgttt tttgcggcgg
     5041 cgtatatcgg cgcactgacc ggacgcagct cgatggaaag cgtgcgcctg tcgttcggca
     5101 aacgcggttc agtgctgttt tccgtggcga atatgctgca actggccggc tggacggcgg
     5161 tgatgattta cgccggcgca acggtcagct ccgctttggg caaagtgttg tgggacggcg
     5221 aatcttttgt ctggtgggca ttggcaaacg gcgcgctgat tgtgctgtgg ctggttttcg
     5281 gcgcacgcaa aacaggcggg ctgaaaaccg tttcgatgct gctgatgctg ttggcggttc
     5341 tgtggctgag tgccgaagtc ttttccacgg caggcagcac cgccgcacag gtttcagacg
     5401 gcatgagttt cggaacggca gtcgaattgt ccgccgtcat gccgctttcc tggctgccgc
     5461 tggccgccga ctacacacgc caagcacgcc gcccgtttgc ggcaaccctg acggcaacgc
     5521 tcgcctatac gctgacgggc tgctggatgt atgccttggg tttggcagcg gcgttgttca
     5581 ccggagaaac cgacgtggca aaaatcctgc tgggcgcagg tttgggtgcg gcaggcattt
     5641 tggcggtcgt cctgtcgacc gttaccacca cttttctcga tgcctattcc gccggcgcga
     5701 gtgcgaacaa catttccgcg cgttttgcgg aaacacccgt cgctgtcggc gttaccctga
     5761 tcggcacggt gcttgccgtc atgctgcccg ttaccgaata tgaaaacttc ctgctgctta
     5821 tcggctcggt atttgcgccg atggcggcgg ttttgattgc cgactttttc gtcttgaaac
     5881 ggcgtgagga gattgaaggc tttgactttg ccggactggt tctgtggctg gcgggcttca
     5941 tcctctaccg cttcctgctc tcgtccggct gggaaagcag catcggtctg accgcccccg
     6001 taatgtctgc cgttgccatt gccaccgtat cggtacgcct tttctttaaa aaaacccaat
     6061 ctttacaaag gaacccgtca tgacccgtat cgccatcctc ggcggcggcc tctcgggaag
     6121 gctgaccgca ctgcagcttg cagaacaagg ttatcagatt gcacttttcg ataaaggctg
     6181 ccgccggggc gaacacgccg ccgcctatgt tgccgccgcc atgctcgcgc ctgcggcgga
     6241 agcggtcgaa gccacgcccg aagtggtcag gctgggcagg cagagcatcc cgctttggcg
     6301 cggcatccga tgccgtctga acacgcacac gatgatgcag gaaaacggca gcctgattgt
     6361 gtggcacggg caggacaagc cattatccag cgagttcgtc cgccatctca aacgcggcgg
     6421 cgtagcggat gacgaaatcg tccgttggcg cgccgacgac atcgccgaac gcgaaccgca
     6481 actcggcgga cgtttttcag acggcatcta cctgccgacc gaaggccagc tcgacgggcg
     6541 gcaaatattg tctgcacttg ccgacgcttt ggacgaactg aacgtccctt gccattggga
     6601 acacgaatgt gcccccgaag acctgcaagc ccaatacgac tggctgatcg actgccgcgg
     6661 ctacggcgca aaaaccgctt ggaaccaagc ccccgagcac accagcaccc tgcgcggcat
     6721 acgcggcgaa gtggcgcggg tttatacgcc cgaaatcacg ctcaaccgcc ccgtgcgtct
     6781 gctgcatccg cgctatccgc tctacatcgc tccgaaagaa aaccacattt tcgtcgtcgg
     6841 cgcgacccaa atcgaaagcg aaagccaagc ccccgccagc gtacgttccg ggctggaact
     6901 cttatccgcg ctttatgccg tccatcctgc cttcggcgaa gccgacatcc tcgaaatcgc
     6961 taccggcctg cgccccacgc tcaaccacca caaccccgaa atccgctaca gccgcaaacg
     7021 ccgcctcatc gaaatcaacg gccttttccg ccacggtttc atgatctccc ccgccgtaac
     7081 cgccgccgcc gtcagattgg cagtggcact gtttgacgga aaagacgcgc ccgaacgtga
     7141 tgaagaaagc ggtttggcgt atatccgaag acaagattaa agccgtctga aaggaacccc
     7201 atgaccttcc tgccactcaa atccccgctc aaattctacg ccgtcgtccc caccgccgat
     7261 tgggtggggc gcatggtcga agcaggtgcc gacacggtgc aactgcgctg caaggccctg
     7321 cacggcgatg aattgaaacg cgaaatcgcc cgctgcgccg cagcctgccg tggcagccgt
     7381 acgcagcttt tcatcaacga ccactggcgc gaagcgatag aagcaggcgc gtacggcgtg
     7441 catctcggac aagaagacat ggacaccgcc gacctcgccg ccattgccgc tgccggtttg
     7501 cgcttgggtt tgagtacgca ctccgtcgcc gaactcgacc gcgccctgtc cgtgcacccc
     7561 agctacgtcg ccagcggcgc gattttcccg accacgacca aacaaatgcc caccgcccct
     7621 caaggcttgg acaaactgtg cgagtatgtg aaacaggcag gcggcacgcc cgtcgtcgcc
     7681 atcggcggta tcgacttgaa caacgcccga gccgtactcg ccaccggcgt ttcctcactc
     7741 gccgtcgtcc gcgccgtgac cgaagcggca aatcccgaag cggtggttaa agcgtttcag
     7801 gctttgtggg atggataaaa ccgaaagaag aaaattcaat tgccgtgtag gcaaaactta
     7861 gcccgttatc gcaaacatac ttaactttaa atgtggcata tcatcaaatt ccgtcatttc
     7921 cgcgcaagcg ggaatccgcc ttaaaacttg agaaaccatc atttgaaaaa cagtttccga
     7981 atttcaaaaa tggattcccg cctgcgcggg aatgacggca actggccggt tgcgtatcaa
     8041 aaaataaagt aattcggc
//
LOCUS       DAXHSJ010000093         8042 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000093 DAXHSJ010000000
VERSION     DAXHSJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.093"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(68..814)
                     /locus_tag="ACFM8U_001383"
     CDS             complement(68..814)
                     /locus_tag="ACFM8U_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6334616.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(798..977)
                     /locus_tag="ACFM8U_001384"
     CDS             complement(798..977)
                     /locus_tag="ACFM8U_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334617.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(993..1664)
                     /locus_tag="ACFM8U_001385"
     CDS             complement(993..1664)
                     /locus_tag="ACFM8U_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC6334618.1"
                     /translation="MQIKKIMKWFPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFM8U_001386"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFM8U_001386"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC6334619.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFM8U_001387"
     CDS             complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFM8U_001387"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC6334620.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFM8U_001388"
     CDS             complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFM8U_001388"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC6334621.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTLGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTLKRAEGGQISATVDRSG
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFM8U_001389"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFM8U_001389"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC6334622.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAEHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="ACFM8U_001390"
     CDS             complement(4571..5881)
                     /locus_tag="ACFM8U_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6334623.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLSETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFM8U_001391"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFM8U_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC6334624.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="ACFM8U_001392"
     CDS             complement(6056..6355)
                     /locus_tag="ACFM8U_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334625.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="ACFM8U_001393"
     CDS             6706..7902
                     /locus_tag="ACFM8U_001393"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6334626.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTAGSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         1897 a         2401 c         1963 g         1781 t
ORIGIN      
        1 ataaatttac cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaattaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa accttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaagg aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccatataccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg ctcggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcatagt cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgca cgctgacttt cctgccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgcc
     1501 cgtcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacagggcga cgggaaacca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatatcgga atcggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tattccgttt tcgagtacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggttt gtcgatattc aagccgtgga catcgacgat ttggttgccg taaaaagtgg
     1921 tgtagccgat acgcaattca ggctgtccgc ccatagcgag cagtttgcgc gaaacaaggg
     1981 aacgcgcgct ttcgacgact tccacgggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag atgaattttt ctgatgcgct ggcgacgatt ttttcgttga
     2101 ggtgcgcgcc gccgccgccc ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 cgatatagac cgccaaaccc gatacttcgt tcagggaaac gacgggaatg tcgtactggg
     2221 caagcagttc gctggatttt ttggaggttg aaaccgcgcc tttgattttt ttgccgctct
     2281 tgcccaaggc ttcgatgaaa aagttgatgg tcgagccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cagcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acatttattt
     2461 gcacgtcctg cccgccgcgt tcaaatccgt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tacctttgat gttgacacac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcgccaag cagcgcgtcg
     2821 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg gtttgaattg aagttcagac
     3001 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggcggat gcccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagcccgg cgcggaaaag
     3181 ctgcggcgtt tgcgcctgcc aaagccccga acggtcgaca gttgcactga tttgtccgcc
     3241 ttctgcgcgt ttgagcgtat cggcaacggg aactgccaaa attccgcctt cggcagcgtt
     3301 gcccgcctgt tctatcaacc gcgtcagagc ttcagacggc aggcagcatc gggcggcatc
     3361 gtgtaccaaa atattgtcgg tttccgccgc caaaccggtt tccaacaatt ttgccacacc
     3421 gtttcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacgccctca tgccgttcaa aaatcccaag cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgttc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcaccacg
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctctatc gtcagaccgt gcaccctcgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcta aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc ggcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaagt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaggcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcagcc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacaggg caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc gtttcactca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacagacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga tacggtcaga tattcgttac
     6121 gcaaagttcc gacgggagct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat gcccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgctagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaagacc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc cattaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaacaaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aggcacgctt tggcaacgcc
     7801 ggcgtgatta ccactgccgg cagcaaagcc gttgccgtgg tcattccgac caacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggcttgt aaggttttat ccgcacacga
     7921 actgcctctg gaaacggagg cagttttttt atccggcttt ccatgcttaa acagcactgc
     7981 ctcttttcag acattgacgg ttgcagccgc ttacctgaac cttatagtgg attaaattta
     8041 aa
//
LOCUS       DAXHSJ010000094         7945 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000094 DAXHSJ010000000
VERSION     DAXHSJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.094"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(65..1312)
                     /gene="fabF"
                     /locus_tag="ACFM8U_001394"
     CDS             complement(65..1312)
                     /gene="fabF"
                     /locus_tag="ACFM8U_001394"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC6334627.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="ACFM8U_001395"
     CDS             complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="ACFM8U_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC6334628.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            1936..2943
                     /locus_tag="ACFM8U_001396"
     CDS             1936..2943
                     /locus_tag="ACFM8U_001396"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC6334629.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNMVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGEDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            2959..3138
                     /locus_tag="ACFM8U_001397"
     CDS             2959..3138
                     /locus_tag="ACFM8U_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334630.1"
                     /translation="MPSKRTFCRSDGIFISFFRLPPLENPLRAALFEIRQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            3276..3599
                     /locus_tag="ACFM8U_001398"
     CDS             3276..3599
                     /locus_tag="ACFM8U_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334631.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            3686..3967
                     /locus_tag="ACFM8U_001399"
     CDS             3686..3967
                     /locus_tag="ACFM8U_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334632.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            4020..4493
                     /locus_tag="ACFM8U_001400"
     CDS             4020..4493
                     /locus_tag="ACFM8U_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC6334633.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            4602..4838
                     /locus_tag="ACFM8U_001401"
     CDS             4602..4838
                     /locus_tag="ACFM8U_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334634.1"
                     /translation="MKRLEDMIMGFWDSAANLAKEAGEGVKRFNERSRKLQNEYQQES
                     SEKLRRVVNSEGFFGNSAQEKAIARKVLKERGEG"
     gene            5035..7725
                     /gene="glnE"
                     /locus_tag="ACFM8U_001402"
     CDS             5035..7725
                     /gene="glnE"
                     /locus_tag="ACFM8U_001402"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC6334635.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFTYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYSAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDQQTQTLPASPEQQQLLAESMGFDSYAAFSDDL
                     NAHRTKVNQLFNEILSEPEEQTLDTSEWQWAWQEKPDEEERQGRLKEHGFDAETIAAR
                     LDQIRNGHKYRHLSAHAQLRFDAVVPLFVQAAAEQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDALRRFQHAQVFRLAVQDLAGLWTVEPLSDQLSALADTILAAAL
                     PCAWADMPKKHRDTPQFAIVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSTATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTVLAGEIIEMRERMFPTHPPVDSNVKYAHGGV
                     VDVEFIVQYLILAHARKYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATA"
BASE COUNT         1967 a         2031 c         2111 g         1836 t
ORIGIN      
        1 ctataaaaat gccgtctgaa acggtttcag acggcatttc gatgtcggcg gcggctttgc
       61 ggaatcagcc tttgaagcgt ttgaagacca gcgtgccgtt ggtgccgccg aagccgaagg
      121 agttggaaat ggcaacgtcg atttccgcgt cgcgcgcttc gttggcgcag tagtccaaat
      181 cgcagccggc ttcaacgtct tgttcaaaaa tgttgatggt cggcgggatt ttgccgtcgt
      241 gtatcgccaa aatgctgtac acggcctcca cgccgcccgc cgcgccgagc aggtggccgg
      301 tcatggattt ggtcgagctg acgacggttt tgtaggcgtg ttcgccgaac gcgcgtttga
      361 gggctttggt ttcgttggca tcgcccaagg gggtggacgt gccgtgcgcg ttgacgtaat
      421 ccacgtcttc gggattgatg ccggcatctt tcagcgcgcg ggtaacggca agggcggggc
      481 cttcttcgtt cggcgcggtg atatggtaag catcggaact catgccgaag ccgacgattt
      541 cggcgtagat tttcgcgccg cgttttttgg cgtgttccaa ttcttccaac accaatatgc
      601 ccgcgccttc gccgataacg aagccgtcgc ggcctttgtc ccacggacgg gaagcggtgg
      661 cggggtcgtc gttgcgggtg gagagggctt tcatcgcggc aaaaccgccc acgcccaaag
      721 tgctgattgc gccttccgcg ccgccggcaa ccattatgtc cgcgtcgccg tatttgatca
      781 gtcgggcgga attgccgatg gcgtgcgcgc cggtggtaca ggcggaaacc atcccgtagc
      841 tcgggccgcg gtagcctttg agaatggtaa cgtgtccgga aatcaggttg atcagcgaac
      901 cggggataaa gaaagggttg attttgcgcg cgccgccttc gattacggct ttgccggtga
      961 cctcgatgct gggcagtccg ccgatgccgg aaccgatgtt cacgccgatg cggtctttgt
     1021 cgaggttttc cacatcgtcc aagcccgaat cggcgattgc ctgcaatgcg gcggcaatgc
     1081 cgtagtggat gaatacgtcc atccggcgcg cttctttcgc gctgatgtat tgtccgatgt
     1141 caaaaccgcg cacttcgccg gcgacacggc tgttgatgtc ggatgcgtca aagcgggtaa
     1201 tcgcgccgat gccgcttttg ccggcgagca gggtgtccca agcctctgcg acagtgttgc
     1261 cgacagggga aacctgacct aagcctgtaa tgactactct tctctgactc atgataacct
     1321 cgctgttggt tgtcggaatg ggggcatatg cggctgtcgt gcagatgccg tctgtaattt
     1381 tcggcagggg ttcaaacagt ttgccatata agggaaaagc ctctattgcg cggtgcagca
     1441 gaggctgttg tgtcgggcga cgaccggtta gccgttgtgg gcattgatgt agtcgatagc
     1501 cagttggacg gtggtgattt tttcggcatc ttcgtcgggg atttcgcagc cgaatgcttc
     1561 ttccaaagcc ataaccagct ccacggtgtc caaagaatcc gcgcccaagt cgtcttggaa
     1621 agaagattcg tttttcacgt cggcttcgtt tacgcccagt tgttcagcaa caattttttt
     1681 aacttgttgt tcgatgtttg acatatcagt cgttccttta tgccttgcgg caggttgttt
     1741 aagggaaata aatcggtggt attgtaccga cttttaatag agttttctat ctaatgacta
     1801 ttatatcaat atttgccgat ttgtacattt ttgggtgcgg cgggttttgt cgttcaagtt
     1861 tgacctgtgt gccgtatgtt tgacgggatt tcggttaaaa tgacggcatt tccatctgaa
     1921 gcagaaagcc ctgtcatgta tccacttgcc cgccgtatcc tgtttgcact cgatgccgag
     1981 aaagcccacc acttcacgct cgacgcgctc aacatggttt ataaattggg tctgattccc
     2041 gtaaccggca accgtaccaa acctgtaaaa ctgatgggtt tggatttgcc caaccctgtc
     2101 gggcttgccg ccggactcga taaaaacggc gaatatatag acgcattggg cgcgctcggc
     2161 ttcggtttca tcgaaatcgg cacggtaacg cccaacccgc agcccggcaa cccgcagccg
     2221 cgcctcttcc gcgttcccga atatcaaggc atcatcaacc gcatgggctt caacaaccac
     2281 ggcatcgatg ccatgattca aaacatcgaa aaaagtaaat tcagcggcgt attgggcatc
     2341 aacatcggca aaaacgctgt aacaccgatt gaaaacgctg ccgatgatta tttaatctgc
     2401 ctcgaaaaag cctacgcaca cgcaagttac attaccgtca atatttcctc gcccaacact
     2461 aaaaacctcc gcgcgctgca aggcggcgac gagttgagcg cattgcttga ggctttgaaa
     2521 aacaaacagg cacagcttgc ctctgtacac gggaaatacg tcccgctcgc cgtcaaaatc
     2581 gcccccgatt tggatgaagc acaaattgaa gacatcgccc acgttgtcaa atccgtcgaa
     2641 atggacggca tcatcgctac caataccacc atcgacaaat caagcctcgg tagccatccg
     2701 ctcgcaggcg agcagggcgg tttgagcggg ctgcccgttc atgaaaaaag taatcgggtg
     2761 ttgaagctgt tggcagacca catagacggc aagctgccga ttatcggcgt aggcggcatt
     2821 atggaaggcg aggatgcggc agagaaaatc cgcttgggcg cgaccgccgt ccaagtgtac
     2881 agcggattga tatacaaagg tccggcattg gtcaaagaat gtttgaaggc tttggcgcga
     2941 tgacgcgtcc cgcccaaaat gccgtctaaa cgcacgtttt gccgttcaga cggcattttc
     3001 atttcctttt tccgcctgcc gccccttgaa aatcccttac gcgccgccct gtttgaaata
     3061 aggcaaacgc ccgccgcgaa ccggacaatc ttgatacgca gccaaagcaa gtccggcggt
     3121 aagttgttaa atatataagc aattgataca aaaaataatt ttatactgtg gcggacggct
     3181 tgggaaaacg caaaatatga acggcgttga aattcatgat gtggaaaata aggatacggc
     3241 ggataatcaa aagattttat atttaaggat ttgatatgtt taaggacgaa ctaaatgaat
     3301 ttatccgatt gatatccgat ccggaaagtg aattggatga atggtatttg agtgatttta
     3361 aagatgaaca tatatgggaa atgcaaagtt acgaagcatt ttcatgtttg cgggaagcag
     3421 tcccctattt gtttgcttat ccccgatacg gatatgaact gttggagata atttccgcgt
     3481 taaaagaaac atctgatacg acagaattat tttatgagcc gggtatcgtg cctttgctga
     3541 ttgatttata taaagaagat agttatctgg taaatatggt caaacggata ttcaaatgat
     3601 gaaaggggcg cgggcgtttg cttggcggaa ggcaggtaac tcaatttgaa ggacgcggaa
     3661 gccgatacgt taaaaaggat gaaaaatgga aaacgggcaa cggaataacc ggtttccttt
     3721 ggaaaaacga attttttatc tggagcattc ggggcggtat ctgatgattt gcgccttatc
     3781 ggattattcc caaaacaaac atactgtcgt tatggcaaat ttcatctatc cggatgaaaa
     3841 aacggattgg cggaatttgg atgatttatt caatgagttg gttttagagg aattgcaggc
     3901 ttcatttatg gattggcatc cgactgttga agaggcaatc agccgtcatt tggaagactt
     3961 ttcgtaacag gcgtcaatcg gaaggggcgg aatgacgggg caggcaatcg gagtaaaaaa
     4021 tgaaccttga tttaaccgcg caaaaagtcc gtctttcttg gaaggatatt ctgtgggggt
     4081 atgggaataa atacttgggt tgggctgatg tggcagctta tgcccgaaaa atgacgcttt
     4141 cagatcatga tgaacgtgtg ttcaaactat ctttaatcaa caaatccaat attcttgaat
     4201 taaagcctgt tctggaagat ttggcttcgg aaatgaggga ttattcccct aaaaattggc
     4261 tgtacgtcct cttaagcgat gtattccata gaaaagaaga atttgaggat cctttggggg
     4321 aagttgaaaa aatttatgca gattttgatt atccggaaga aatagaatca tttgtcaggt
     4381 atatgccgcc caaagacggt tatattcctt ctgcccacac ctatgaagaa aatattgccc
     4441 ggttatattc tcactgggaa cactatttga acaacggcgg agggcagggt taaaaccggc
     4501 aatccgatgc cgtctgaagc attatccggc cttcagacgg cattttgttt tccgacagtt
     4561 tataaactgt cgttgtttct tgacagaaac aacgacctta tttgaaacga ttggaggaca
     4621 tgattatggg tttttgggat agcgcggcaa atttggcaaa agaagcagga gaaggcgtaa
     4681 agcgatttaa cgagagaagc cgtaaattgc agaacgaata tcagcaggaa tccagtgaaa
     4741 aattacgccg cgtcgtaaac agcgaaggtt ttttcggtaa ttcggcacaa gaaaaagcca
     4801 ttgcccgcaa agttctgaaa gaacgtggcg aaggctaaac gagggcagcg catttgccgc
     4861 tgttttttta tcggtaggta tccgtccgaa tatcggggta aggtttcaga cggcatcgaa
     4921 ggttgctatg atatagtggc ttgactttaa accggtacgg catcccctcg ccctgtcctg
     4981 atttgaagtg aatccgccat catcttcctg ccatccttcc aaacggaatc cgaaatgtcc
     5041 gacaaccgcc tcgacaccgc ccgccgccat tccctgttcc ttgcccgcca gctcgacaac
     5101 ggcaaactca agcccgaaat attcctgccg atgttggaca aggttttgac cgaagcggat
     5161 ttccaagcct ttgccgactg ggacaaaatc cgtgcggaag aaaacgagga agaattggcg
     5221 cggcagttgc gcgggttgcg ccgttatgtg gtgtcgcaga ttatcgtgcg cgatatcaac
     5281 cgcatcagcg atttgaacga agtaacccgc acgattacgc tgtttgccga ttttgccgtc
     5341 aataccgcgc tggattttgc ctacgcctat tatcgggaca tgtacggcac gccgatcggg
     5401 cgttatacca aatcgccgca gcatttgagc gtggtggcga tgggcaaggc gggcggctat
     5461 gagttgaacg tgtcttccga catcgatttg attttcacct atcccgaatc gggcgacacc
     5521 gacggcaggc gcgaacgggg gaatcaggag ttttttacca aagtcgggca gaaactgatt
     5581 gcgctgttga acgacatcac cgccgacgga caggtgttcc gcgtcgatat gcggctgcgg
     5641 ccggacggtg attcgggcgc gttggtattg agcgaaaccg cgctggagca atatttgatt
     5701 acgcaggggc gcgaatggga acgctacgcg tggtgcaaag gccgcgtggt tacgccgtat
     5761 ccgaacggaa tcaaatcgct ggtgcgccct ttcgtgttcc gcaaatattt ggattacagc
     5821 gcgtatgagg cgatgcgtaa gctgcaccgc caaatcagca gcgaagtcag caaaaaaggc
     5881 atggcggaca acatcaaact cggcgcgggc ggcatccgcg aagtcgaatt tatcgcccag
     5941 attttccaga tgatacgcgg cggccaaatg cgtgcgctgc aactgaaagg cacgcaggaa
     6001 acgctgaaga aacttgccga aaccgggatc atgccgtctg aaaacgtcga aaccctgctt
     6061 gccgcctacc gattcctgcg cgatgtcgaa caccgcctgc aatactggga tgaccagcaa
     6121 acccaaaccc tgccggcttc gcccgaacag cagcaactgc ttgccgaaag tatgggtttc
     6181 gacagctacg ccgctttttc agacgacctc aatgcccacc gcaccaaagt caatcagttg
     6241 ttcaacgaaa ttttgagcga acccgaagag caaaccctag acaccagcga atggcaatgg
     6301 gcatggcagg aaaagcccga cgaagaagag cggcaaggtc gtctgaaaga acacgggttc
     6361 gatgccgaaa ccatcgccgc aaggctcgac caaatccgaa acggtcataa ataccgccat
     6421 ctctccgcac atgcccagct gcgtttcgat gcggttgtgc cgctgttcgt acaggcggcg
     6481 gcagagcaaa gcaacccgac cgatacattg atgcggctgt tggattttct cgaaaacatc
     6541 agccgccgat ccgcctatct cgccttcctc aacgaacatc cgcaaacctt ggcgcaactg
     6601 gcgcagatta tgggccaaag ttcttgggtg gcggcgtatc tgaacaaata tccgattttg
     6661 ttggacgaac tcatcagcgc gcagcttttg gataccgcgt ttgattggca ggcactcgcc
     6721 gccgcccttt cagacggcat cgaagcctgc ggcggcgata ctgaagcgca aatggacgcc
     6781 ctgcgccgct tccagcacgc ccaagtcttc cgtctcgccg tccaagacct tgccggactg
     6841 tggacggtag aacccctctc cgaccaactc tccgccctcg ccgacaccat actcgccgcc
     6901 gccctgccgt gcgcatgggc ggacatgcca aaaaaacacc gcgacacacc gcaattcgcc
     6961 atcgtcggct acggcaaact cggcggcaaa gaactcggct acgcctccga cctcgacttg
     7021 gtctatcttt atgacgaccc gcatcccgat gcaggcgacg tgtacagccg ccttgcccgc
     7081 cgcctgacta actggctttc caccgccacc ggcgcaggca gcctctacga aaccgacctg
     7141 cgcctgcgcc ccaatggcga tgcaggtttc ctcgcccaca gcatcgccgc ctttgaaaaa
     7201 taccagcgcg aaaacgcatg gacgtgggaa caccaatccc ttacccgcgc ccgttttatc
     7261 tgcggtacgc ccgaaattca gacggccttc gaccgcatcc gtaccgaaat gctgaccgcc
     7321 gaacgcgacc aaacggtttt agcaggcgaa atcatcgaaa tgcgcgaaag aatgttcccc
     7381 acccatccgc cggttgacag caacgtcaaa tacgcgcacg gcggcgtagt agacgtcgaa
     7441 tttatcgtcc aatatctgat acttgcccac gcccgaaaat atccgcaact cctcgacaac
     7501 tacggcaaca tcgccctctt gaacatcgcc gccgactgcg gtttgattga taaaacgctt
     7561 gccggacaaa gccgcaccgc ctaccgcttc taccgccggc agcagcacaa caccaaactg
     7621 cgcgacgcgg caaaaaccga agtaaccggc gaactgttgg cacattacgg caatgtcagg
     7681 aaattgtggc gggaagtgtt cggcgaagaa gcggcaaccg cctgaacaaa aaatgccgtc
     7741 tgaagcctga caatctgggt ttcagacggt attttcgtac cgtgccgttt taaggttgcg
     7801 gcagagctaa agcgatttat cgggaatggc tgaaacccaa aaaccggatt cctctttcgc
     7861 gggaatgacg ggatttcagg cttctgtttt tgtgggaatg atgggatttt ttatccaagc
     7921 aaaaatcaaa acaaacaaat aagaa
//
LOCUS       DAXHSJ010000095         7923 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000095 DAXHSJ010000000
VERSION     DAXHSJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.095"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..479)
                     /locus_tag="ACFM8U_001403"
     CDS             complement(<1..479)
                     /locus_tag="ACFM8U_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6334636.1"
                     /translation="MIASRLSGNISSAIQEHKDGKVNINRFQNILADLYSLGGLGSTL
                     IEKNGNMQSWGIPLAIAGDIIAATAIATGDTGTISTEEFYNFDNWKGFGYELFEDWSR
                     WVYDWLPDGWNLWKELDRNRSGQYHIYDPLALDLDGDGIETVATKGFAGALFDHRNQG
                     "
     gene            complement(697..1416)
                     /locus_tag="ACFM8U_001404"
     CDS             complement(697..1416)
                     /locus_tag="ACFM8U_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334637.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
     gene            complement(1692..2126)
                     /locus_tag="ACFM8U_001405"
     CDS             complement(1692..2126)
                     /locus_tag="ACFM8U_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334638.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            2463..3152
                     /locus_tag="ACFM8U_001406"
                     /pseudo
     CDS             2463..3152
                     /locus_tag="ACFM8U_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(3284..3799)
                     /locus_tag="ACFM8U_001407"
     CDS             complement(3284..3799)
                     /locus_tag="ACFM8U_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334639.1"
                     /translation="MSAFRKILLIISCLLIASCSFVETIFYMAISPEPVVVDFPLGKK
                     TKRSIELKQKIGKPYAISLGTNFIHYDPKQGERWIDDKLNYPYNISVKIFKVEEDGKK
                     LIIDELLTERSRKLGVGVFGAGGKYSMHIYDFYLPEGEYLFEISDNSEYIPLYDEINN
                     SIRIVVNVRIQ"
     gene            complement(4060..4617)
                     /locus_tag="ACFM8U_001408"
     CDS             complement(4060..4617)
                     /locus_tag="ACFM8U_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpA"
                     /protein_id="HFC6334640.1"
                     /translation="MENNKAKSDDNSVSIEFIQDFITASTDVINLSYENFRKNFYTQM
                     STDSTNYAAKHESLGKSVQRELQKTQSQLRQVVRKMQSKYNINNKARAAEISLLRQMQ
                     SQFSRKDVNKNLGNGNTLAQQGSYTKKDGTTAQAGDLLLAADNLHSRLTDKMLSISHV
                     RENTISPFVLGCLKQITINVSHYLK"
     gene            complement(5154..5438)
                     /locus_tag="ACFM8U_001409"
     CDS             complement(5154..5438)
                     /locus_tag="ACFM8U_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334641.1"
                     /translation="MKLALKRSGKYNVYNPLALDLDGDGIETVAAKGFSGSLFDHTNN
                     GIRTATGWIAAYDGFPVRKLNSNGGIISTTDTIFQSLHTWLDHQPNDTCH"
     gene            complement(5435..5728)
                     /locus_tag="ACFM8U_001410"
     CDS             complement(5435..5728)
                     /locus_tag="ACFM8U_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HFC6334642.1"
                     /translation="MNIDDFTPSKIANFFADPDTYSNVLEEVSRFIYSLVPDDANPWK
                     GGEDYIGRGISEWGELLEKWYKQDFLPYLEKNGTNFRNLKIGCLNSLNGQESG"
     gene            complement(6066..6287)
                     /locus_tag="ACFM8U_001411"
     CDS             complement(6066..6287)
                     /locus_tag="ACFM8U_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HFC6334643.1"
                     /translation="MTSANFNINGFGDVKLTPYSPLLGYKAWDSFIGSIQSLSDLIYN
                     VDNNRNKMEITVNNAIQAADSFLSSNWKR"
     gene            complement(7146..7923)
                     /locus_tag="ACFM8U_001412"
                     /pseudo
     CDS             complement(7146..7923)
                     /locus_tag="ACFM8U_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein FrpC"
BASE COUNT         2578 a         1482 c         1217 g         2646 t
ORIGIN      
        1 ccttgattgc ggtggtcgaa caatgcacct gcaaagcctt tggtggcaac ggtttctatg
       61 ccgtctccgt ctaggtctag ggcgaggggg tcgtagatgt ggtattggcc tgaacggttt
      121 ctgtccaatt ctttccacag attccagccg tcgggcagcc agtcgtatac ccaacgagac
      181 cagtcttcaa atagctcata cccaaaacct ttccagttgt caaaattata aaattcctct
      241 gtagatatcg taccagtatc tcctgtggca atagccgttg ctgcaattat atctccagca
      301 attgctaatg gaatccccca actctgcata tttccattct tctctattaa tgtacttcct
      361 aaccctccca atgaatataa atccgctaaa atattttgaa aacggttgat attaacttta
      421 ccatctttat gctcctgaat agctgaagat atatttccag agagacggct tgcaatcaca
      481 gaggcatttg ttccgtttac aatatgcgat gacgtactac ctggtgcaat attactagcc
      541 aaattaccaa atgctaataa tgcactataa ccagcttgtt tgtaatattc attacgtttc
      601 gagatatctt tctcattttt tgcagaaact aaattagcaa ccatatttcc aaattctgct
      661 aacgcggatg agcgtgcgct cataaaatta ccaaaactat tccaactcat tgaagtaagt
      721 cctatataaa agcaaattaa attttaaata taaaataatt aatacgatac aaacactgtt
      781 ttcaataaga aaaaatgttt tatacttatc taacaagtac aaacttttgt ttccatcatt
      841 aatttcaaat attacattat ctataaaatc tccttgaggt tgattataaa aataaatttt
      901 aacaggtttt tttgcaaatt ttataaaatt ggcacatgga gcatatgaat gaataccaca
      961 aaccaagctt aatcttatgg atttattttt aacatcagat gcaactacat caatataagg
     1021 tgtttctcta attaccctac catatctatg attatctttt aatttagcat atccatttga
     1081 gtcataaaca taattataac tatcatgtgg ttttatcacc ccagtaatcg aggatatatt
     1141 ttgttccttt atttgatgct caaatttata aaattttata tttatcatac taatatgaat
     1201 taccatccat ataataatag aaatcggcaa taaaaacaat aatttgtttt ttatgttttt
     1261 ttctaaaaat aataaattta cagcaagaaa gacgtttgca aaaaacaaaa acgcaaaaaa
     1321 atcaaataga aaaacaaaat aaccaaacac tatattaatt actatcaagg ctatatgtaa
     1381 aactataaat actagtaaat aatttttatt tttcatatgt ctcattaaat aaaatatagc
     1441 tggtatgatt tagtgaatat caatttagtg gcatagagtg tatactcatc gtattcactc
     1501 tctcagtctt caggctgcct gaaaagcctt tggcggtgac tgtttctatg ccgtcgccgt
     1561 ccaagtctag ggcgagggga tcgtagacgt ggtattttcc atcacacgca gaaaattttt
     1621 tgcattgttt agtatcacca ctaccaatca ttgttactct ccttgagttt aagtcaaaca
     1681 aaaaatgaaa ttcaaatact ctctcttaac ttattgagat caatcatcaa tataaataaa
     1741 aaatataaac taccagaata accaaagcca cgaaaaattt tttccaatat ttcactattt
     1801 aaacaaaaag caccgcatac ccacgttgat gattccgaaa taaaacacat ccatatttcc
     1861 attactgcca accatgacac tactatccta atcaaaaata cttttttatc tgcccaagca
     1921 ctttttatat ttaacaatac aatatgattt tgtaatttaa aatctaagct ttgcattttt
     1981 ctaaccagtg caaacaaaca accaagcgca ataaaaaaag attggtagga ctggtaaaaa
     2041 ggtagaaata gaatatttaa gaccaatgga tagatccaca tatacctatt tgttaaatta
     2101 ttaataaatt cttttttgtt atccataaaa tttccataaa atattgcgct gatattgcac
     2161 tgagtttatt agcaaacaac gtaaagaaaa tatttattat gataagtgtt ttttaagttt
     2221 cattttcaaa tgatggcgcg taataaaaag tttcacaaat ccgtttttca gactatctaa
     2281 gtatctattt tacaatctat ttcaataatc gttatgtctt aacaactctt cttggcatta
     2341 tagatgctca ctcatatcat ttaatgatac tcttgattaa tctttatgcg atttagcgaa
     2401 attaaaattt ctcttttttg tttgaggctg tactagatta gcagatatgt tacccccgaa
     2461 atatgaagat aacacactgc aaattaaaga aaaaagtaca gaaagagctg ctccgttttt
     2521 ttgtactcga agttaccgcc cgttctgctg ccgatatttt gggtatccat cccaattcgg
     2581 cagtcctgtt ctaccgcaaa atccgtatag tcatcagcca ttatctggcc ttggctgcca
     2641 atgaggtttt cgagggcgct gtcgagttgg acgaaagcta tttcggcgga cggcgtaaag
     2701 gcaaacgtgg tcgcggtgcg gcaggaaaag tggttgtctt cggcattctg aaacgtaacg
     2761 gacgggtcta taccgttgtc gtagataatg ccaagtccga tactttaatg cctgttatca
     2821 aacagaaaat catgccggac agtattgttt ataccgatag tctgagcagc tatgacaagc
     2881 tgcacgtaag cggttttatc cattaccgca tcaaccattc caaggaattt gctgaccgtc
     2941 agaaccacat aaacggcatt gagaactttt ggaatcaggc aaaacgcgtc ttgcgcaaat
     3001 agaacggaat tgatcgcaaa tctttcccgc tgttcttgaa agaatgcgaa tttcgattta
     3061 acttcggcac accgtcccga cagctaaaaa tcttgcggga ttggtgtgga tttagggcta
     3121 atctagtaca gccccgattt ttttatctct agccgaatac agttcaaccc aaacgtaccc
     3181 gtcttggata cacccccttg tttcagtggc gtaccctgct ccgcaaattt tacagttatc
     3241 gcataataat cagccataag taaccccaca tttctagaaa aatttactga attcgtacat
     3301 taactactat tcttatagaa ttatttattt catcgtaaag tggaatatat tcactattat
     3361 cagaaatctc aaataaatat tccccttccg gcaaataaaa atcataaata tgcatactgt
     3421 attttccccc agctccaaat actccgaccc ctaattttct acttctctct gtaagcaact
     3481 catctataat aagtttttta ccatcttctt ccactttaaa tattttaacc gatatattat
     3541 atggatagtt taacttatca tcaatccacc tctccccctg ttttggatca taatgtataa
     3601 aattagttcc taacgatatt gcataaggtt taccaatttt ctgtttgagt tcaatagatc
     3661 tttttgtttt tttaccaaga ggaaagtcta ccacaacagg ttctgggcta atagccatat
     3721 aaaaaatagt ttcaacaaaa ctgcagctag caatcaatag gcaagatatt attaataata
     3781 tttttctaaa tgcagacata ataaaaattt ccaaaaaatc catatcagaa tatactaatt
     3841 atttgtataa ttaataagga agagaaagac tcaaaacggc tatgagattt ccgcctgaaa
     3901 ataatgaaat aaaagtccat agtaatgaat aatagcttta tcagcccccc ctttaatgca
     3961 aacatagaga atacttccct tgaagcacta tcaatttcta cttaactaaa actctagctc
     4021 tattttaaaa ttttgtcaaa aatagttaaa tacttttatt tattttaaat aatgagatac
     4081 atttatggtt atttgtttta gacagcctaa aacaaacggg gaaatcgtgt tttcccgaac
     4141 atggctaatg gatagcattt tgtccgtgag gcggctgtgc aggttgtcag cagccaacag
     4201 caaatcgcct gcttgtgcgg ttgtgccgtc ttttttggtg tagctgcctt gttgagccaa
     4261 agtgttgccg ttaccaagat ttttgtttac atcttttcga gaaaattggc tttgcatttg
     4321 ccttaacaaa gatatttctg ctgctcgtgc tttattattt atattatatt tactctgcat
     4381 ttttcttaca acttgtctca actgactttg tgttttttgt aattcacgtt gtaccgattt
     4441 tcctaaactt tcatgtttgg ctgcataatt ggtagaatca gttgacattt gtgtataaaa
     4501 atttttacga aaattttcgt aactcagatt aattacatcc gtacttgcag ttataaaatc
     4561 ttgtataaat tcaatagaaa ctgaattatc atcagatttg gctttgttat tttccatatt
     4621 gagcctctct taatttgaaa aaattaacat aaacatttaa aaataaaaca aaattatata
     4681 ataaaaaaaa taatgagaat ataaaggata aaaagagagt taagttattt aatcggactt
     4741 gttctctata aatcataagg caccaaataa gccataataa atttagtaaa taaaaactgc
     4801 ttatacccaa ctttcttttc ttggaaccta ttattatttc aactctggat gaaaaaatag
     4861 aatttaaaaa tttttttctt tgatatatcg ttaatatctc cctagttttt cttaaaaaaa
     4921 tataatcata tatatactac acaacattgg taataaataa attaaaagcg aaaggtaatt
     4981 aaattgataa ttgtaaatta tataataaac aacatacgaa gaaaaaatta ttatagatga
     5041 tttgctatac gaataaaaaa atatcctacc tagcccgaac cttcttataa tttgtactat
     5101 gtgatcattc atttcaatat ccatttaagc tttcaattaa ttttctatat ttttcaatgg
     5161 catgtatcat ttggttgatg atcaagccat gtatgcaaag attggaatat ggtatctgtc
     5221 gtgctaataa tgcccccgtt actgtttaat ttgcgcacag gaaaaccgtc atatgcagca
     5281 atccagcccg tggcggtgcg gatgccgttg ttggtgtgat caaataagct gcctgaaaag
     5341 cctttggcgg cgacagtttc tatgccgtca ccgtccaaat ctagggcgag gggattgtaa
     5401 acgttatatt tgcctgaacg tttgagagct aatttcaacc actctcttgc ccattcaggg
     5461 aattcaggca gccaatcttc aaatttcgga aattggtccc attcttttca agataaggga
     5521 gaaaatcttg tttataccat ttttccagta actctcccca ttcacttatc cctcgtccaa
     5581 tataatcttc gccccctttc caagggtttg catcatcagg aactaaggaa tatataaacc
     5641 tagatacttc ttctaataca ttgctgtatg tatcaggatc cgcaaaaaag tttgctatct
     5701 tacttggtgt aaaatcatct atattcatta tagattcgcc aagcctctcg tttccataat
     5761 ccttatttaa ttcaattcat gctgtaaaac agtagaggat tttagcattc tagaattaag
     5821 gacatcattt aaaccagttt ttaatttagt tggtaagaat ttgctttgag ccatcgcctc
     5881 tgtttgttgt tttatatatt gtaatccatc accagcagca atgaatatat caccaattgc
     5941 ttgttgaata tcattaggtt taaattttcc tgtttctcgt atatctcgag atacatttct
     6001 taaatttaaa aaatgttatc gaaggatgca agtaaagaag cagtatttgt tattttgtta
     6061 ttttgttatc tcttccaatt actgcttaaa aagctatctg cagcttgaat agcattatta
     6121 acagtaattt ccattttatt tctattgtta tccacattat agattaaatc agataaggat
     6181 tgaatagaac caataaatga atcccaagct ttatatccca agagtggtga atagggtgtt
     6241 aatttcacat ctccaaaacc gttaatatta aaatttgcag atgtcatatc tgctttcctt
     6301 tcaagtaaat taaaaaaata acgtgaggaa ttaaaattaa gaataaatta attatcgact
     6361 caatataata tgaatcaatt agctctctta tggtaaatat aagaggtaaa aatattaaaa
     6421 ataaactaat aaagttcaga aaaatagtaa atggaatatt ttcatatgga aaaattctac
     6481 aaaatacaag taatattcca tgacatatta gcaaatgagt aaaatttatt ataaaatata
     6541 taaatatttc cccatcgtaa gatatattca aataaagaca tataaacaat atgaagtata
     6601 taaaatatat tttttggcat ctaaaaaata aacttttagc aatcatatat aaatagcttg
     6661 tagaataaaa aacgaaaatt attaattatc gtaggtgtgc atttattcca acacggtttc
     6721 atgcagctta aaaaccaaaa attctttctc cctttatcct ttcttaaaaa tagcgttttc
     6781 aaattctgca acgacaggtt tcattcaagt aggactgccg tgattgcatc aattgcttcg
     6841 cggcatggca tccatatcac acaacaggac atttattctc gggtaagtcc accttcatcc
     6901 tcttccgcca gcaggaacag ttacaggcta cctaaaatat gctctgtgct cggatagtaa
     6961 atatttcctt tctcactatt gtttgtcatt taatcgacag tctactcttt ctctaaaatt
     7021 tcgtcaaaaa tagttaaata cttttattta ttttaaataa tgatatacat ttatggttat
     7081 ttgtttagac agcctaaaac aaacggggaa atcgtgtttt cccgaacatg gctaatggat
     7141 agcattttgt tcgtgaagcg gctgtgcaga ttgtcggctg ctaaaagtaa atcccccatt
     7201 tttgcggttg taccgtctgt tttggtgtag ctgccttgct gagccaaagt gttaccgtta
     7261 ccgagatttt tatttacatc tttataggcg agatccaaag attgaatacc caattcttca
     7321 agggtacgca attcattagc ttgggaaatg ccgtcctgat tgagatcctg ccatacacgc
     7381 agggattgga atgcggcgtc tgccgcgttg atgatgttgt cgccgtttga atccaattcg
     7441 gccaaagctg catagccgtg tttggcaaaa gaaccgtctg ccagtttggt gttgtcgccg
     7501 aagagttccg cgccgttgtc gatgatgccg ttgccgttca aatcgcggac gagtaaaccg
     7561 tcatcggcag aaacccaacc ggtggcggtg cggatgcctt gattgcggtg gtcgaacaat
     7621 gcacctgcaa agcctttggt ggcaacggtt tctataccgt ctccgtccaa atctagggcg
     7681 agggggtcgt agacatgata tttgccattg cgttttttag tttctttaac ccaaggagcg
     7741 aaacattggt gaccatcatc gatccagcct tccggatttg ggaacagatc tctaaattgc
     7801 ttgatgttat tattaacgat ctcaaatatt ccagtccact ctctttttgc aacactttta
     7861 tataaatcat tgattatccc attaccccaa cctccaattt tttgccaacc atctattgta
     7921 tat
//
LOCUS       DAXHSJ010000096         7895 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000096 DAXHSJ010000000
VERSION     DAXHSJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.096"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(51..2093)
                     /gene="recG"
                     /locus_tag="ACFM8U_001413"
     CDS             complement(51..2093)
                     /gene="recG"
                     /locus_tag="ACFM8U_001413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC6334644.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAIGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQVREIAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2179..2622)
                     /gene="secB"
                     /locus_tag="ACFM8U_001414"
     CDS             complement(2179..2622)
                     /gene="secB"
                     /locus_tag="ACFM8U_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC6334645.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDADLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2643..2900)
                     /gene="grxC"
                     /locus_tag="ACFM8U_001415"
     CDS             complement(2643..2900)
                     /gene="grxC"
                     /locus_tag="ACFM8U_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC6334646.1"
                     /translation="MQTVTMYTGPFCPYCTMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3114..4607
                     /gene="rng"
                     /locus_tag="ACFM8U_001416"
     CDS             3114..4607
                     /gene="rng"
                     /locus_tag="ACFM8U_001416"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC6334647.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4642..5892)
                     /locus_tag="ACFM8U_001417"
     CDS             complement(4642..5892)
                     /locus_tag="ACFM8U_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6334648.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(5905..6062)
                     /locus_tag="ACFM8U_001418"
                     /pseudo
     CDS             complement(5905..6062)
                     /locus_tag="ACFM8U_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6285..7598)
                     /locus_tag="ACFM8U_001419"
     CDS             complement(6285..7598)
                     /locus_tag="ACFM8U_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC6334649.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            complement(7703..>7895)
                     /locus_tag="ACFM8U_001420"
     CDS             complement(7703..>7895)
                     /locus_tag="ACFM8U_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334650.1"
                     /translation="IPRVQHAPRSAMIGAVSFESAHRDECPRAVPIPICRLFRIGCRR
                     EMTDGCRNGGRREWEEMTD"
BASE COUNT         1865 a         2335 c         2045 g         1650 t
ORIGIN      
        1 aaatcagatt tccgttacac ttttttccaa tatttcagac ggcattttgc tcacacgccc
       61 aaataccctt ccctgccgga aagccacctt gccaaatgcg cttcgacgat ttcggggttt
      121 tgctcaatca gcatcggggc gatttcgcgc acttgttcca aaaggtgtaa gtcttcttca
      181 agcttggcga agcgcagcat aggcacgccg ctttggcgcg cgccgagaaa ttcgccgggg
      241 ccgcggatat tgaggtcttg gcgggcgatt tcaaagccgt cggtgtgttc gtaaatcact
      301 ttcaaccgcg ctttggcgag ttcgcccaag ggttcggcaa acaggaggac gcacacgctt
      361 tctgccgcgc cgcgcccgac ccgaccgcgt aattggtgca gctgcgccaa gcccatgcgc
      421 tcggcgtgtt cgatgaccat cagggcggca ttgggcacat ctacgccgac ttcgatgacg
      481 gtggtggcga ccaagacgtt cagccccccc gaagaaaacc gcgccatcac ttcggccttt
      541 tcggcggcct tcatgcgccc gtgtaccagt ccgatattga gttcgggcaa tgccgtctga
      601 agccgggcga gggtttcggc ggcggtttgc agttgcaggg tttcgctttc ttcaatcaat
      661 gggcagaccc aatacgcctg ccgccctttt cggcaagtgc cgaggacgaa gccttcgact
      721 tcggcacggc ggacgttgtt gacgaggcgc gttttaatcg gtgtgcgccc gggcggcaat
      781 tcgtcgatga cggacacgtc caaatcggcg aaaaaactca tcgcaagcgt gcgcgggatg
      841 ggcgtggcgg acatcatcag ctgatggact tcgcgccctt tgtttttgag ggcgaggcgt
      901 tgggcaacgc cgaaacggtg ctgttcgtcc acaatggtca agcccaaatt gtgaaacgcc
      961 acgccgtctg aaaacagggc gtgcgtgccg acggcgattt tgacgctgcc gtcagccagt
     1021 ttggctttgg cttcgtcttt ggctttttta cgcaaactgc caaaaaggcg gacaacttca
     1081 atgcccaaag gttcgagcca ttgtttaaat ttaataaaat gctgttcggc aaggatttca
     1141 gtgggcgcca ttacagccac ctgcgcaccg gattcgatag ccgtcaaagc agacaaagca
     1201 gccacaatgg ttttgccgct gccgacatcg ccctgcagca ggcggtgcat cgggtaggtt
     1261 tgcgccatat cgcggcagat ttcggaaaca actttttctt gcgcatcggt cagggcaaac
     1321 ggcagggctt ggcgcagggc ttgggtcaat gtgccgtcgc cgcccaatgc cgccgccgtg
     1381 ccgccgatac gcttctgtcg cgccaagcgc atcgaaagct gttgcgccaa aagttcgtcg
     1441 aatttgagcc gttgccatgc aggcagcgtg ccgtctgaaa gctgatgaat cgtgaaactc
     1501 ggcggcggcg aatgcaaaag acgcaggctt tcggcgaggt gtggcagctt cagacggcac
     1561 agcagggcat cgggcagcgt gtcgtgcagc ggcgtaacgt ccaacgccgt ctgaataata
     1621 cggcgcaaag tgggctggtt caaaccgttt acggtcgggt aaaccggcgt gaggctttcc
     1681 gccaaaccgc cgccctcggc atcgcggatt ttgggatgaa tcatctcgtc gccgtaaaag
     1741 ccgtgtttga tttcgcccac ggcgcggatg cgtttgccga tcgccgtctg tttctgatgg
     1801 ctggcgtaaa agtggatgaa gcgcagaaaa aggacgctgc cggagccgtc ggcgatttgg
     1861 acaatcagct gcttgcgcgg tttgaacgtt acttcctgat ggataacctc cccctcgacc
     1921 tgacacggca cgccaatcgg cgcgtcctta atcggcataa tgtgcgtctc gtcctcgtaa
     1981 cgcagcggca ggtgcaacac caaatcccac gcggtatgga ggttgagttt gtcgagcttc
     2041 ttggcggaaa catcggtgat tttgagctgt tttcgggttt cgggcgacat cataggcaga
     2101 ttcctttgga cgcgcctatt ttatccgaaa acaaaaatgc cgtctgaaac ggattcagac
     2161 ggcatcgaca ggcaggaatc aagctccggc ggcttcggct tcctgctgtt gttggtaaat
     2221 cgcctcaaaa ttaatcggcg cgagcaggac gggcgggaag ccggcgcgcg ttaccgtacc
     2281 ggaaacggct tcgcgcgcgt aagggaagag gatgttcgga cacgccacgc cgagcagcag
     2341 gtcggcatct tcttcgggga tgttttccaa acggaaaata ccgctttggg ttacttcgtt
     2401 caaaaacatc gtgcgctcgt tatccaattt ggcggttacg gtaacggtta catccacgtt
     2461 gtagtagccg tcttccagct tttggctgcc ggtggaaacg cgcatatcca cttcgggctc
     2521 gccctgttcc aaaaagattt gcggcgcgtg cggcacttcc aaagacaagt ctttgacata
     2581 cagtcgctcg atgctgaata cgggttgcag ttcttcgctc attttgtttt cctagttggg
     2641 ggttaagggt tcagcagtcc gtccagcccg ccttcctgct ggaggcggta gaggtcggta
     2701 aatccgccga cgtgcgtttc gccgatgaaa atctgcggca cgctgcgctg tccggaaagc
     2761 tgctgcattt cggcaaaggc ttcgggactt gcatcgacac ggatttcgtc gatatgtccg
     2821 acacctgccg cgtgcagcag ccttttcgcc atcgtgcagt aggggcaaaa cggacctgtg
     2881 tacatggtaa cggtctgcat attgggtttc cgaaagtttt gcaatgataa tcaatatagg
     2941 ggcatttccc ccgtttggca agtgcggaac agatgcacgt tcaaacggca tgtgcggaat
     3001 gtgtcaaagt ttctttttta aagtatgata gacattgtga aaaatatttt tgcacccgcg
     3061 ctgcgcggcg gaacggatgc aaaaatattt ttattacatt ttcaggaaaa accatgttgt
     3121 caggactccc catccccaaa gacatcgcgc gcccgcccga aacgatattg gtcaacatca
     3181 cgccgcagga aacgcgcgta gcggtgttgg aggaaaacaa tatctgcgag ctgcacatcg
     3241 agcgcaacag cgaacacagc ctagtcggca atatctattt gggcgtggtg cgccgcgtgc
     3301 tgcctgggat gcagagcgcg tttatcgaca tcggcttgga acgcgcggcg tttttacaca
     3361 tcgtcgatgt cctcgaacaa cgccgcaacc ccgaagaaac ccagcgcatc gaacatatgc
     3421 tgtttgaagg gcagtctgtt ttggtgcagg tcatcaaaga cccgatcaac accaaaggcg
     3481 cgcggctttc cacccaaatc tcgctggcgg ggcgtttcct cgtccatctt ccgcaagaag
     3541 accacatcgg cgtgtcccaa cgcatcgaag acgatgccga acgcagcagc ctgcgcgaac
     3601 gcctcgacaa gctcctgccg gaaaatgcct gccggggcta catcatccgc accaacgccg
     3661 aaaacgccac cgacgaacag ctccagtccg acatcgacta cctgaccaaa gtgtgggaac
     3721 acatccaaga acaggcgaaa atccggccgc cggaaaccct gctttatcag gatttgccct
     3781 tgagtttgcg cgtgttgcgc gatatggtcg gctgcgacac gcaaaaaatc ctcgtcgatt
     3841 ccaccgtaaa ccacggccgc atgacgcgtt ttgccgaaca atacgtccac ggcgcattgg
     3901 gcaggataga attgttcaaa ggcgaacgcc cgctatttga aacccacaac gtcgaacagg
     3961 aaatcagccg cgccctgcaa ccgcgcgtca acctcaactt cggcagctac ctgattatcg
     4021 aatccaccga agcgatgacc acgatagacg tgaacacagg cggcttcgtc ggcgcacgca
     4081 atttcgatga aacgattttc cgcaccaacc tcgaagcctg ccacaccatc gcccgcgaat
     4141 tgaggctgcg caacctcggc ggcatcatca tcatcgactt catcgatatg gcgcaggaaa
     4201 gccaccgcga agccgtgttg caggagcttg ccaaagccct cgccttcgac cgtacccgcg
     4261 ttaccctgca cggttttacc agcctgggac tggtcgagct gacgcgcaaa cgctcgcgcg
     4321 aaaacttaaa ccaagtcctc tgcgaaccct gcccttcctg ccaaggcaga ggccgtctga
     4381 aaacgccgca aaccgtgtgc tacgaaatcc agcgcgaaat cgtccgcgaa gcgcgccgtt
     4441 acgatgccga aagtttccgc atcctcgccg cccccaacgt catcgatttg tttttggacg
     4501 aagaatcgca atccttggca atgctgatag atttcatcgg caaaccgatt tctctggcgg
     4561 tcgaaaccgc ttacacgcag gaacaatacg atatagtgct gatgtaaaaa atgccgtctg
     4621 aagccttcag acggcatctg tctatttcag ggtttccttg tccaacaacg cccgtatcaa
     4681 cagaccgtgt ccgaaacgtc ggctgtcggt cagttccaaa tgtccgccgt attttttggc
     4741 aagcgtgtcg gcgatggaca gacccagccc cgtcccctgc tgctccgttc ccaaaatacg
     4801 gtaaaacgga tcgaggacgc gggcgcgttc ggattcggga atgccgttcc cgttatcttc
     4861 cacccacacg gcaagatatt tcccttcgtc tgtgaaaccc aaatctatcc tgccttcgga
     4921 cggggtataa cgtattgcgt tatcaacaaa cgttttaacc aaggtataga tttcagtttc
     4981 atcggcaaat atttcgacat cacctccgac cgccacacca atgtcctgac gtttttccaa
     5041 ggcaagcggc atcagttcct gcaacacttg gcggaaacgg ctttgcagac cgaatgtcgt
     5101 tttcgccaaa ggggtttcgt ccgactgcga acgcgccagt gccaaaagct gctcgagcag
     5161 gtgtttgttg cgccgtatgc tttgctgcaa aacggcagac tgccgcgccg catcgggtgg
     5221 cagagacata ttgttgagcc gttccgcctg aagggaaagg gctgtcatcg gcgtacgcaa
     5281 ttcgtgtgcc gcgtcggcga caaaccgctg acggtggcgg atgtcttcat ccgcacgttt
     5341 caaaagcaag ttgatggcgg ttacgaaccc tctgatttca ctgggaatat tgtccacact
     5401 caaggcagac aggtcattga ttcggcgttg ttcgagactt tgcgacaatt tgcggacggg
     5461 gcgcatggct ttgtgcgtaa tccacacggt cagcagtatc atcagcggca gtgccgccag
     5521 caggggcaac acgctttgcc gtgccgcatc cgccgccaaa tcttcccggt attcgctttc
     5581 ctgcatcacg gcaatccgcc cccgctccgt cgtgcggata tagacgcggt aataatcgtc
     5641 gtccccgtcc gcctgaagcg tgtgcagacc gtccgccaga tgcgcaggca ggctgacaac
     5701 agggtcttcc tgctgcggca tctgtaccaa aatacgcgta tcgccgtcgc cctcgggcaa
     5761 agtttcgggt ttggaatcgg gggcgacgta caatgccgcc tgacggagca ggtcgtcctg
     5821 caacgcttcc gtttcgtgga aggtttcgta gtaggaaaac atacctgcaa gcattgccag
     5881 cggaacaaac atccaaaccg tccgccccgg caaacccaaa tccagcagca tcaagtcata
     5941 aggctgggca gcggcagccg cgcgccgttt ttgacccaat ccaccgcata gccgccgtct
     6001 ttcaaacttg ccgacaccgc ctccgcaatc atcgcatcgt cttccaccag caaaacacgc
     6061 atcaactttc cctttcagac aaaccgtgcc tattctaaca ccccaaaatt agccgcaatt
     6121 tagcggcctt tacgcttgcc ggtatttttc aaaactgcag cacaaaaaaa ccgcgccggc
     6181 aactgccttc agacggcatt ggggcgcgat tgcaacacac gggcaggcag gcagagcctg
     6241 cgacagacca caggaacgat tcgggcttca gacggcttcg ccgtttacgg cagaggcacg
     6301 attcctgccg ctatcgaact ggccaatatc gccagcgaca aaccccacgc ccaaaaaaac
     6361 gaataacgga tgtgtttgcc catcgacaat ttcgccaaac ccaagcccat ccacaaagcc
     6421 ggcgaaagcg gcgtaacaaa agtgccgacg atactgccga tcaacatcgc ataacctgct
     6481 gcttcgggcg ccacgcccgc ctgcgaggta atctgctcca caatcggaaa cagtccgaaa
     6541 taataagcgt ccgtactcaa aaccaactca agcggaatgc ccaacacacc gatggcaata
     6601 tgcagataag gcagcagcgc gtccggcagg atatgcacaa tgtctttgga aatcgcgtcc
     6661 aacatccccg cacccttcaa aatccccaaa aacgtacctg ccgccaaaat aatggacgcc
     6721 atcatcaccg cgccgccggc gtgggcataa atccgctcca tctgttcctg cgggctgcgg
     6781 taattcaaaa gcaacgccgc cgttgcagcc agcataaata cataacccgg cgggaagatg
     6841 cccgaaaaaa gcaggctcat cgccgccaaa aacagcagga cattccacca aaacagtttc
     6901 ggacgcgcca atttttgttc ttcttccgac aaaggcaccg gctttatcaa atccgccacg
     6961 gcgggcaacg cgcccaactc ccggacaatc cgcctttttt cacgcacacc caaaagcagg
     7021 gacagcgcaa ggataaacac tacaccgata atttgcaccg tcaacaaagg tttatacaat
     7081 tcgcccacat ctgcgcccaa cacgcttgca acccgcccgg tcggcccgcc ccacggcaga
     7141 aggttaatca atcccgcact ggaagtcagc agcaaaaaca gcaggtaagg attcatatgc
     7201 agacgcttgt aaagcggcaa aagggcgggg acgaccaata aaaacgtcgt cgcacccgcc
     7261 ccgtccaact gcgccaccac cgacaccaag accgtcccca cactcactgc cacgatatta
     7321 ccccgagtca gcttaatcaa accgcctatc atcggacgga acagccccac atcgttcatg
     7381 attccaaaaa acaaaatgga aaacataaac ataatcacaa tctgcatcac cgatttggtg
     7441 ccgcccgaat aaaattcttt taattgggat acatcaaacc ccgccagcaa cgccccaaac
     7501 agcggcacca agattaatgc gatgatgggc gacacttttt ccgtcagcag cagccatacg
     7561 atgaccccga taatcagcag tccgataaac gtcagcatca tttctccttt attttatttt
     7621 aaacagaaaa ccgaccgtgc aggcaaaacc gcccacagac gcgggataag ccttgcattc
     7681 tactttttta ttttgaaaca agtcaatcgg tcatttcctc ccattcacgc ctgccgccat
     7741 tcctgcatcc gtccgtcatt tcacggcggc aaccgatacg gaacaaccgg caaatcggta
     7801 tcgggacggc gcgggggcat tcatcccggt gcgccgattc aaacgaaacc gcccctatca
     7861 ttgcggagcg cggggcatgc tgtacacgcg ggatt
//
LOCUS       DAXHSJ010000097         7895 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000097 DAXHSJ010000000
VERSION     DAXHSJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.097"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..110)
                     /locus_tag="ACFM8U_001421"
     CDS             complement(<1..110)
                     /locus_tag="ACFM8U_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC6334651.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWTI"
     gene            388..1542
                     /gene="obgE"
                     /locus_tag="ACFM8U_001422"
     CDS             388..1542
                     /gene="obgE"
                     /locus_tag="ACFM8U_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC6334652.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1625..2674
                     /locus_tag="ACFM8U_001423"
     CDS             1625..2674
                     /locus_tag="ACFM8U_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6334653.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3362..4783
                     /gene="cysS"
                     /locus_tag="ACFM8U_001424"
     CDS             3362..4783
                     /gene="cysS"
                     /locus_tag="ACFM8U_001424"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC6334654.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFSAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAAFDLSENANDYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4866..5645
                     /locus_tag="ACFM8U_001425"
     CDS             4866..5645
                     /locus_tag="ACFM8U_001425"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6334655.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5632..5976
                     /locus_tag="ACFM8U_001426"
     CDS             5632..5976
                     /locus_tag="ACFM8U_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334656.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            5987..6517
                     /locus_tag="ACFM8U_001427"
     CDS             5987..6517
                     /locus_tag="ACFM8U_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6334657.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGDVPRDWAQKQADTWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6746..7861)
                     /gene="asd"
                     /locus_tag="ACFM8U_001428"
     CDS             complement(6746..7861)
                     /gene="asd"
                     /locus_tag="ACFM8U_001428"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC6334658.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         2079 a         1961 c         2033 g         1822 t
ORIGIN      
        1 taaatcgtcc acaggacaat ggtggtcaga atgccgatga aaccggcaac ggtcagccag
       61 tgcagcgcgc gtgtcaacgg gtcgtaaacg gcaggttggt ttgtgttcat aaaaaactta
      121 ttatatgtaa cggtttgaac ggacattccg tcgggtcgga atgtcaaaaa ggcgcgattg
      181 taccaaagaa gttggacata attttgtttg caggctgaag atttgcttaa aaattcatta
      241 agatgggcgg aacaaatagt ttgggtacgg cgtgttgaaa taccgcttat cccttataat
      301 agcgtccgaa attctgttca gacggcatca aaacacacgg taatccgtct gaacccccca
      361 tttgaaacca acaaacaaga gcattgaatg aaattcatcg acgaagcaaa aatcgaagtc
      421 gccgcaggca aaggcggtaa tggcgcaacc agtttccgcc gcgaaaaatt cgtaccgcgc
      481 ggcggcccgg acggcggcga cggcggcaaa ggcggtagcg tctgggcgga agccgacgaa
      541 aacaccaaca ccctcgtcga ataccgcttc gtcaaacgat accaagccaa aaacggcgaa
      601 aaaggccacg gttccgaccg ttacggcgca ggtgcggacg acatcgtcct caaaatgccc
      661 gtcggcaccc tcatccgcga cctcgacacc gacgaaatcg ttgccgacct cacttaccac
      721 ggacagcgcg tctgcctagc caaaggcggc aaaggcggct tgggcaacat ccacttcaaa
      781 tcctccgtca accgcgcccc aaaacaatcc acgcccggcg aagaaggcga agcccgttcc
      841 ctgcaactcg aactcaaagt cctcgccgat gtcggcttat tgggtatgcc caacgccggc
      901 aaatccaccc tgattaccgc cgtatccgcc gcacgcccca aaatcgccaa ctaccccttc
      961 accaccctgc atccaaactt aggcgtggta cgcatcgacg aaaaccacag cttcgtgatg
     1021 gccgacatcc ccggcctgat tgaaggcgcg gcagaaggcg cgggtttggg gcatcgtttc
     1081 ctcaaacact tatcgcgcac cggattattg ctgcacgtcg tcgatttggc gcccttcgac
     1141 gaaaccgtca accctgccga agaagcactt gctatcatca acgaattgcg caaatacgat
     1201 gaagaacttt acggcaaacc gcgctggctg gtgctgaata aactcgacat gcttgacgaa
     1261 gaagaagccc aaacgcgcac tgccgccttc ctcgaagccg tcggctggga ctaccccaaa
     1321 cccgacgacc gtttccaatt tgacatggaa accccgcgcc tcttccaaat cagcgcgctg
     1381 acccaccaag gcacgcagga attggtacac caaatcaacc aatacctgac cgagaaaaaa
     1441 cgtatcgaag ctgaaaaagc cgaagcggag aaagcagcgg caaatgttga aattatcgaa
     1501 cagcagccta aaacggatac gggcgtgttt aagccggagt gaaaaaagaa agggtttcag
     1561 acgatatttg ttctacaaaa ggttgtttga aacaatttta aagcaagatt gaaatggatt
     1621 aaaaatgtca tctaaccaat atatccagtc gcttgagttg acaaacttta ccgttcttcc
     1681 aaacgatact tttgaatttt ctgaaaatct caatgttatt gttgccgaaa acggttgtgg
     1741 caaaacacat ttgttaaaaa ttttatacag cctgctagaa gtaacatcta acaccaaaaa
     1801 cagattactg aaaacagaat tgcaaaaaag ttttgccgat aaattactga atgttttccg
     1861 tcccgattcc ttgggcaggt tgagcaaacg tttgcagggg cgcgggcgta cggaaatcgt
     1921 tttaaaactg caaaacggca ctacgcatag tagtctgaat ttttccagta actcatcatc
     1981 gcaagtcaat gttcaaagca tcggattgaa agaaaatgaa tatactccga cacctatctt
     2041 cttacccagc cgcgaattaa ttacactttg cccttggttc acttctttgt atcaaaacca
     2101 aagcatccct ttcgaagaaa cttggtttga tacctgtatg caattaaatc acccgcttgc
     2161 caaaggcccc agagaaacaa aaattcgaga attgctcgaa cctattgaga acgcgatggg
     2221 cggaaaagta tctgaagaac aaggaaggtt ttatctatcg ctgacaaata cgggcggtaa
     2281 gattgaagca cctttggtag cagagggttt gcggaaattt gtcatgattg cccggttaat
     2341 cgcaacaggg gcattgctgg ataaaggcta tctattttgg gatgaaccgg aagctaatct
     2401 gaatcctaaa ttgatcaaag tggctgcccg tattatttgg tctttatctc aacagggtat
     2461 tcaggttttt attgcaacac actctctatt cttattgaga gaattggaac ttttgaagct
     2521 tgaaaataac acggatttac ctgtacgttt tttctcgttg attgcttccg atgatggaac
     2581 aaaagtggag caagggaaca gtattaatga tttgaacaca ttgatattgt tagatgaaaa
     2641 cattatgcag gcagacaggt atctgaatat ttgaggttaa gctatgacta caatcacaga
     2701 aaagagatta acctttgctt ttcctgaaga ttattacgtt accaagtatg acgaatggga
     2761 acattataaa atttttcaga acagttgtaa tttacgtaat aaaattgata caaatgaaaa
     2821 gggaaagaac gggataaatc aaagtgtaga tgacgataat ggcagctcag gtgtagatat
     2881 aatagccctt catgagtcga cactttggct aatcgaaatt aaagattatt accgacttgg
     2941 gcttgagcca aatgcccaat caatagatga aaaactttct gacctaccat atttaatcgc
     3001 aagaaaaatt cgagattcat tggcaggatt ggtttctgct aaatttaaag cagaaaaaca
     3061 ggaagaaaaa gatttttctc gcttggcttt agattgtaac gaaataaaaa tagtccttca
     3121 tattgaaatg cctaatataa gaagtaaact ttatccctcg tcatcagatt tagcaaactt
     3181 attaaaagat aagtttaaat tatcagaatt tacaaaaaat tttgcaaact gttatgcaga
     3241 accaattttc acaaatatca accatattaa taatccgcaa ctacgtaatg ttccttggtc
     3301 cgtatctaca ggaacagaac agaaattatc atccgagcaa caaagattaa tccataatcc
     3361 catgaccacc atctacaaca cccttacccg ccaaaaagaa ccctttgccc ccatcgaccc
     3421 tgaaaacgtg cgtatgtacg tttgcggcat gactgtttac gactattgcc acttaggcca
     3481 tgcccgtgtg atggtggtgt tcgacatgat tgcccgttgg ttgcgtgagt gcggttatcc
     3541 gctcacttat gtgcgcaaca tcaccgacat cgacgacaaa atcatcgccc gtgcggctga
     3601 aaacggcgaa accatcggcg aactgaccgc gcgtttcatt caggctatgc acgaagatgc
     3661 cgatgcttta ggtgtgttgc gtccggatat tgagccgaag gcgacggaaa acattccgca
     3721 aatgattgcc atgattgaaa ccctgattca aaacggcaag gcatatcctg ccgcaaacgg
     3781 cgatgtttac tacgccgtgc gcgagttttc cgcttacggc caattgtcgg gcaaatcgtt
     3841 ggacgacctg cgcgcaggcg agcgcgtgga ggtggacggt ttcaaacgcg atccgcttga
     3901 ttttgtgttg tggaaagcgg ccaaagcagg cgagccggca tgggaaagcc cgtggggcaa
     3961 tggccgtccg ggttggcaca ttgaatgctc cgccatgagt gaaaacctgt tcggcgatac
     4021 tttcgacatc cacggcggcg gtgcggattt gcagtttccg caccacgaaa atgaaatcgc
     4081 ccaaagcgtc ggtgccagcg gtcatacctg cggccacgac cacgcgcaaa cccatcacgg
     4141 tcaaagcatc gccagccacg tcaaatactg gctgcacaac ggcttcatcc gcgtggatgg
     4201 cgaaaaaatg tcgaaatcgc tgggcaactt cttcaccatc cgcgaagtat tgaaacaata
     4261 cgaccctgaa gttgtgcgtt tcttcatcct gcgcgcccac taccgcagcc cgttgaacta
     4321 ttccgacgcg catttggacg acgcgaaagg cgcgttgacc cgtctataca ccaccttgaa
     4381 aaacacgcct gccgccgcgt ttgatttgtc tgaaaacgcc aacgactaca cccgccgctt
     4441 ctacgccgcc atgaacgacg atttcggtac ggttgaagcg gttgccgtgt tgtttgagct
     4501 ggcaggcgaa gtgaacaaaa ccaatgacgc acagctcgcc ggctgcctga aagccttggg
     4561 cggcatcatc ggactgctgc aacgcgaccc gaccgagttc ctgcaaggcg gcgcggcttc
     4621 agacggcctc tccaacgaag aaatcgaaga cttgatcgcc cggcggaaac aggcgcgcgc
     4681 cgataaaaac tgggcggaat ccgaccgcat ccgcgacctt ctgaacgaac acaaaatcat
     4741 tttggaagac aacgccggcg gcacgacttg gcggcgcggt taaaatccat cgaaaagggt
     4801 aaatgtgccg cattttgccc ttttttgttc agacggcatt tttattgtga gaacttattt
     4861 ttattatgct taaaatcatt tccgccaacg tcaacggcat ccgctccgcc tacaaaaaag
     4921 gtttttacga atacatcgcc gcatcgggcg cggacattgt ctgcgtgcag gaactcaaag
     4981 cgcaggaggc tgatttgtct gccgatatga aaaatccgca tgggatgcac ggccattggc
     5041 attgcgccga gaagcgcggt tacagcggcg tggcggtgta cagcaaacgc aaacccgaca
     5101 atgtgcaaat cggtatgggc attgaagaat tcgacaggga agggcgattt gtgcgttgcg
     5161 atttcgacag gttgagcgtt atttcgcttt acttgcccag cggcagcagc gcggaagaac
     5221 gccagcaggt gaaataccgt ttccttgatg cgttttaccc tatgctcgaa gcgatgaaaa
     5281 acgaaggacg cgacattgtc gtttgcggcg actggaacat cgcccaccaa aacatcgacc
     5341 tgaaaaactg gaaaggcaat cagaaaaatt caggtttcct gccggaagaa cgcgagtgga
     5401 taggcaaggt tatccacaag ctcggttgga cggatatgtg gcgcacgctt tatcccgatg
     5461 tgccgggcta cacgtggtgg agcaaccgcg ggcaggcgta tgcgaaagat gtcgggtggc
     5521 gcatcgatta tcagatggtt acgcccgaac ttgccgccaa agccgtgtcc gcacacgttt
     5581 ataaagatga aaaattttcc gaccatgcgc cgctggtcat ggagtatgac tatgctgccg
     5641 aataaagttt tgggtaaata tgattggaat gtggacggaa aaacaggaat aggcgcagca
     5701 tgggttgtcg ctgcgtttat cctgcccata ttggtgtggg cgatatttat gttgtcgcgg
     5761 atgcaaggct ggcttgcgcc gaccaaggca aacccgacat gggcgttggt gtggctgctg
     5821 atctgcatgc cctgcctgct gattgcgtcc aaatgtttgg gttggaaagg ctggcggcgg
     5881 gtggtgaata tttttgcctg cctgaccgtc tgcgccatat tgagcgtacc cgcttcgctg
     5941 ctcatcgcct ttaccctccg ggacctgctg aaataggcgg ggcggtatgc agggttttga
     6001 actcgaagat ttgacgcttt ggctgatacg cgatgccgga gaggatcaga tgtggataga
     6061 ccgttgggcg atcagttatc ccgtcgtgca gatgtccgaa gcgtcggccg gtcaaagcat
     6121 aggggaatgg caggcagggc ttcggacggc atttgaacgc atacgtggca aatacgtcgc
     6181 cgttgtcgca cacggtgcgg gcgcggccgc atttttggcg tggctgtatc aggcagacat
     6241 cctgacacgg aagaaaattg ccaacatcat ccttgtaccg cagcgtcccg atatttttcc
     6301 cgacgatgcg gaacacactt tccaacgcgt ccgctgtccc tgccgtgccg cattggtggt
     6361 gtccgaacac ggggatgtgc cgcgagattg ggcgcaaaaa caggcggata cgtggaacgc
     6421 gcgcctgttg gtttccccgc attcgggcag tttgaacggt atgctcggcg gctggcagtg
     6481 gggtatgaag ctgatgcagg aaatgttgct ggcgtgaacg cttgccttat cggaatttcg
     6541 gctgtagcag gaagtccgca tatcgggctg ttttcattga cgtgcaggcg catcaggttc
     6601 ggataaagtc gggctgccgc ataccgccgg aaatggaatc ctttattttt tacgtcggca
     6661 gtgccggcag ggcgttatcc ggcagggttg caaggaaaat gccgtctgaa acaggcttca
     6721 gacggcattt ccattcaaac agggcttaca ggctgcccaa cacgatacgc aatacgcggc
     6781 gcagcggctc ggccgcgccc cacaagagct ggtcgccgac ggtgaacgcg ctgatgtatt
     6841 cgccgcccat gcccagtttg cggatgcgtc cgacagggac ggacagcgtg ccggtaactt
     6901 tggcaggagt cagctcgtgg atgctggctt ctttttcatt ggggatgact ttcacccagt
     6961 cattcgcgcc tgccaaaatc gtttcgattt cggaaacagg caggtctttt ttcaacttca
     7021 gagtgatggc ttggctgtgg cagcgcatcg cgccgacgcg gacgcacagg ccgtcaatca
     7081 cggttggatt gtcgctgcgg ccgaggattt tgttggtttc cacgccgcct ttccattctt
     7141 ctttggactg gccgttgccc aaatccacgt caatccacgg aatcaggctg ccggcgagcg
     7201 gtacgccgaa gttggctttc ggatagtctt cgctgcgcag gaaatcggat actttgcggt
     7261 cgatgtcgag aatcgagccg gcaggatcgg caagttcgtc cgccacttgg gcgtgaaccg
     7321 cgcccatacc gctgatgagt tcgcgcatgt ttttcgcacc tgcgccggaa gcggcttggt
     7381 aggtcatgct ggttgcccat tcgaccaaat cgttttggaa caggccgccc aaagccatca
     7441 gcatcaggga gacggtgcag ttgccgccga tgtagttttt cacgccgttt ttgaggccgt
     7501 tgtcgataac gttgcggttg acagggtcga ggacgataat cgcgtcgtct ttcatgcgca
     7561 gagaagatgc cgcgtcaacc cagtagccgt tccagccgct gtcgcgcagg gcttggaaga
     7621 cggatttggt gtaatcgccg ccttggcagg taacgatgat gtccattttg gcgagttcgg
     7681 caacattgtt ggcatctaat aatgttttag ccgcctgacc gaaatcaggg gctgcgccgc
     7741 cgacgttgga agtggtaaag aaaaacgctt caggaatgtg ggcgaagtcg ttttcttctt
     7801 tcatacgctg catcaaaacc gaaccgacca taccgcgcca gccgacgaaa cctactttca
     7861 ttgtgttgct ccttgaggaa aaatcgttaa tatag
//
LOCUS       DAXHSJ010000098         7746 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000098 DAXHSJ010000000
VERSION     DAXHSJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.098"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            595..1470
                     /gene="prpB"
                     /locus_tag="ACFM8U_001429"
     CDS             595..1470
                     /gene="prpB"
                     /locus_tag="ACFM8U_001429"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC6334659.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDTMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            1555..2709
                     /gene="prpC"
                     /locus_tag="ACFM8U_001430"
     CDS             1555..2709
                     /gene="prpC"
                     /locus_tag="ACFM8U_001430"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC6334660.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTELCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            3035..3979
                     /gene="gntB"
                     /locus_tag="ACFM8U_001431"
     CDS             3035..3979
                     /gene="gntB"
                     /locus_tag="ACFM8U_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis L-arginine gamma (S)
                     hydroxylase"
                     /protein_id="HFC6334661.1"
                     /translation="MYHKKFDKEILLKIKELYRYDNWHAVLAILYDYLIIIFSIFLSE
                     QSYWFLPLTILLIGSRQRALATILHEASHSALTKNKKFGQILGTYFSGYLIFQSWNSY
                     CVSHVKNHHPKLGTDSDPDYKYYKDSGIFQTYSRIEFFWRFCVSQILCLKLFRNFKYL
                     IKNRLFSDITRTELFKLLLSQLILFVIGTYFVGYKFYVIYWLLPYLTAFQMITWFIEL
                     SEHYPMIQTAKNNLEASRNRFSHAIEHFFTGIHGENYHLIHHLFPAIPFWNLEKAHKI
                     LMKDFDYQEVNKDFGGIFFSKNNNAPMWKVLWSKYEAA"
     gene            3966..4877
                     /locus_tag="ACFM8U_001432"
     CDS             3966..4877
                     /locus_tag="ACFM8U_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6334662.1"
                     /translation="MKLRDLLMGIFLAVSAALLNATIGIFSKILMEQGLSVQHIAFLK
                     TLTGFVFISILLCRTGFTRQIADISRKKEAILPLLLKVAICAFFGIYTLFFFETTAYQ
                     YGNAANVVVVLMASAAVSALILDSILLDERICISSVVGVGLAVLGIAMISWTGEGSLG
                     LILNAALAGSGYGCFSVLIKKFGLNGGIYLTRILMFFGSIFLFIPSLEGIEDIHWQWS
                     FIPPLLALSLLPTILGFYCTTKALDYLSAAKVQVTELAEPLFAAVLAWLFLNEIPEGR
                     FFVGAILIIAGIVSINGLYRPLLKRIE"
     gene            4968..7574
                     /gene="acnD"
                     /locus_tag="ACFM8U_001433"
     CDS             4968..7574
                     /gene="acnD"
                     /locus_tag="ACFM8U_001433"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HFC6334663.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRRPGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLQRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGREIRLKDIWPT
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSLARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIAAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVIGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
BASE COUNT         2049 a         1791 c         1866 g         2040 t
ORIGIN      
        1 cttgtcctga tttaaattca atccactata tatttaaatt taattaaata atataaaaat
       61 caaatagata acaaactaca tcggattgct taaaaataat acattgtttt tatgtataaa
      121 atattttata agttttcagg attttgatta tcaaaaattt ttcttgattt cctgacaatt
      181 ttattgaaac aaataattaa aaattaatct agtttaatca tggaattaaa ataaaatatt
      241 aaaattatgt aatgagtctc cttaaaaatg tttgacattt tcagtcttgt gttttagatt
      301 agcgaaaaat aaaactacac aatactacaa aggaacatta ctatgaaacc aattcagatg
      361 ttttcccctt ttctgaataa tccccttgtt ttcttcttgt ctgcggtttt gccgcataat
      421 tccgaacggt ctgctgtttt tctttgattc gttttaaata tcaataagat aattgttccc
      481 atatattttt aatgattgga ttgggatgcc cgatgcgtcg gatggatgtg ttttgccgtc
      541 cgaatgtgat ggaagcctgt ccatactgaa aaaagtctat aaaggagaaa tatgatgagt
      601 caacactctg ccggagcacg tttccgccaa gccgtgaaag aatcgaatcc gcttgccgtc
      661 gccggttgcg tcaatgctta ttttgcacga ttggccaccc aaagcggttt caaagccatc
      721 tatctgtccg gcggcggcgt ggcagcctgt tcttgcggta tccctgattt gggcattacc
      781 acaatggaag atgtgctgat cgacgcacga cgcattacgg acaacgtgga tacgcctctg
      841 ctggtggaca tcgatgtggg ttggggcggt gcattcaata ttgcccgtac cattcgcaac
      901 tttgaacgcg ccggtgttgc agcggttcac atcgaagatc aggtagcgca aaaacgctgc
      961 ggccaccgtc cgaacaaagc cattgtatct aaagatgaaa tggtcgaccg tatcaaagct
     1021 gccgtagatg cgcgcgttga tgagaacttc gtgattatgg cgcgtaccga tgcgctggcg
     1081 gtagaaggtt tggatgccgc tatcgaacgc gcccaagctt gtgtcgaagc cggtgcggac
     1141 atgattttcc ctgaagccat gaccgatttg aacatgtacc gccaatttgc agatgcggtg
     1201 aaagtgcccg tgttggcgaa cattaccgag tttggttcta ctccgcttta tacccaaagc
     1261 gagctggctg aaaacggcgt gtcgctggtt ctgtatccgc tgtcatcgtt ccgcgcagca
     1321 agcaaagccg ctctgaatgt ttatgaagcg attatgcgcg atggcactca ggccgcagta
     1381 gttgacacta tgcaaacccg tgccgaattg tacgagcatc tgaactatca tgccttcgag
     1441 caaaaactgg ataaattgtt tcaaaaatga tttaccgctt tcagacggcc tttcaacaaa
     1501 tccgcatcgg ccgtctgaaa aataaaaccc ataaaaatac aaaggagaaa taccatgact
     1561 gaaactactc aaaccccgac tttcaaacct aaaaaatccg ttgcgctttc tggcgttgcg
     1621 gccggtaata ccgaattgtg taccgttggc cgtaccggca acgatttgag ctatcgcggt
     1681 tacgacatct tggatttggc acaaaaatgc gagtttgaag aagttgccca cctgctgatt
     1741 cacggtcatt tgcccaataa attcgagttg gccgcttata aagccaagct caaatccatg
     1801 cgcggcctgc ctatccgtgt gattaaagtt ttggaaagcc tgcctgcaca tacccatccg
     1861 atggacgtaa tgcgtaccgg cgtatccatg ctgggctgcg ttcatcctga acgtgaaagc
     1921 catccggaaa gtgaagcgcg cgacatcgcc gacaaactga tcgccagcct cggcagcatc
     1981 ctcttgtact ggtatcaata ttcacacaac ggcaaacgca ttgaagttga aagcgacgaa
     2041 gagaccatcg gcggtcattt cctgcaactg ttgcacggca aacgcccaag cgaatcacac
     2101 atcaaagcca tgcacgtttc actgattctg tatgcggaac acgagttcaa cgcttctacc
     2161 ttcactgccc gcgtgattgc cggtacaggc tctgatatgt actccagcat taccggtgca
     2221 atcggcgcgt tgaaaggtcc gaaacacggc ggcgcgaacg aagtggctta cgatattcaa
     2281 aaacgctacc gcaatgccga cgaagctgaa gccgacatcc gcgaacgcat cggccgcaaa
     2341 gaaatcgtga tcggtttcgg tcatccggtg tacaccattt ccgaccctcg caacgttgtc
     2401 attaaagaag tggcacgcgg tttgagcaaa gaaaccggcg atatgcgcct ctttgacatt
     2461 gccgaacgtt tggaaagcgt gatgtgggaa gagaaaaaaa tgttcccgaa tctggactgg
     2521 ttctctgccg tttcctacca aaaattgggc gtaccgaccg ctatgttcac accgctgttc
     2581 gtgatttccc gtacaaccgg ttggagcgca cacgttctcg agcaacgcaa agacggcaaa
     2641 atcatccgtc cgagcgcgaa ctacacaggc cctgaagatt tggcgtttgt ggagattgaa
     2701 gaacgctgag acggaacaca accgtaggag accaccaatc ggatttcaga cgacgtgtag
     2761 cgcgggcttt gcctgcggaa aactgaaacc cgcctacggt tccgcttttc agacggcctg
     2821 ttgaaagatg cgagagagaa ggttgtctga aaaaccagcc gcaacgtctg ccgtcattcc
     2881 cacccccgcc taagcgagga caggtcgcgg cgggaatggc ggaggcagcg cggcaacttt
     2941 tttcacggtt ctgccaaaat atgctatgaa agaatgttcg tcagcataat ggagatgaag
     3001 ccgatattat aactaatacg gagaattgtg ggatatgtac cataagaaat ttgataaaga
     3061 aattctttta aaaattaaag aattatatag atacgataat tggcatgctg tacttgctat
     3121 attatatgat tatttaataa taattttttc tatattttta tctgagcagt catattggtt
     3181 tctcccttta acgattttat taataggaag caggcaaagg gcgttggcaa caattttaca
     3241 tgaagcctct cattctgcct taactaaaaa taagaagttt ggacaaattt taggtacata
     3301 tttttctgga tatttaattt ttcaaagttg gaatagttat tgtgtttctc atgtgaagaa
     3361 tcatcatcca aagttaggga ctgattcgga tcctgattat aagtattata aagactcagg
     3421 tatttttcag acatattcaa gaattgaatt tttctggaga ttttgtgtct ctcaaatttt
     3481 atgtttgaaa ttatttagga attttaaata tttaattaaa aatagattat tttcggatat
     3541 tacaagaaca gagttgttta agctacttct ttctcaatta attctatttg ttatcggtac
     3601 atattttgta gggtataaat tttatgtaat ttattggtta ttaccatatt taactgcatt
     3661 ccagatgata acttggttta ttgagttgtc cgagcattat ccaatgatac aaactgccaa
     3721 aaataattta gaagcgtcaa gaaataggtt tagtcatgca atagagcatt ttttcacggg
     3781 gatacatgga gaaaattatc atctaataca tcatttattt ccagccattc cattttggaa
     3841 tttggaaaaa gcacataaga tattaatgaa agattttgat tatcaagagg taaataaaga
     3901 ttttggaggg attttctttt cgaaaaacaa taatgcacct atgtggaaag tattatggag
     3961 taaatatgaa gctgcgtgat ttactgatgg gaatattctt ggcagtttct gcggcccttc
     4021 tgaatgcaac catcggcata ttcagcaaga tattgatgga gcagggcttg tctgttcagc
     4081 atattgcatt tttgaaaact ttgacaggtt tcgtgtttat cagcattttg ctttgccgta
     4141 ccggttttac cagacagatt gcggatattt caagaaagaa agaggcaatt ttgccgttgc
     4201 tgttaaaagt agcaatttgt gcttttttcg gaatttatac gttgtttttc tttgaaacca
     4261 cagcttatca atatggcaat gctgcgaatg tagtagttgt attaatggca tcggctgccg
     4321 tatctgcctt gatattggac agcatactgt tagatgaacg tatttgcatt tcttcagtcg
     4381 tcggtgtggg tttggcagta ttggggatcg caatgatttc ttggactgga gaaggaagtt
     4441 tagggttgat tctgaatgcc gcactggcgg gctcgggcta cggttgtttt tccgttttga
     4501 ttaagaaatt cggcctaaac ggcggtattt atttgacacg gatattgatg ttttttggaa
     4561 gtattttttt gtttatccct tcattggaag gtattgagga tatacattgg caatggtctt
     4621 ttattccgcc actcttggca ttgtctttat tgccgacgat tttaggattt tattgtacaa
     4681 ctaaagcatt ggattatttg agtgctgcga aggtacaggt aactgaattg gccgagccat
     4741 tgtttgctgc cgtactggct tggttgtttt tgaatgaaat accggaagga cgcttctttg
     4801 tcggcgccat tctgattatt gccggtattg tgtctatcaa tgggctgtat cgaccattgt
     4861 tgaagcgaat tgaataattt ttatttccca agatttcaga cgacctcttc aacaaacgtc
     4921 gtctgaaacc ccaaaaccaa aaaaataata gatttaggag aaccgacatg gctgccaacc
     4981 aacgttaccg caaaccgctg cccggtacgg atttggaata ctacgacgcg cgtgcggcgt
     5041 gtgaggacat caagcccggc tcttacgaca agctgcctta cacgagccgc attttggcgg
     5101 agaatttggt caaccgcgcg gacaaagtcg atttgccgac gctgcaaagc tggctgggtc
     5161 agctgattga gggaaaacag gaaatcgact ttccttggta tccggcgcgg gtggtgtgcc
     5221 acgatattct ggggcagacc gcgttggtgg atttggcagg tctgcgcgat gcgattgccg
     5281 aaaaaggcgg cgatcctgcc aaagtgaatc cggtggtgca aacccagctc atcgtcgacc
     5341 actctctggc cgttgaatgc ggcggctacg accccgatgc cttccgcaaa aaccgccaaa
     5401 tcgaagacag acgtaacgaa gaccgtttcc acttcatcaa ctggacaaaa accgcatttg
     5461 aaaatgtgga cgtgattccg gcgggcaacg gcatcatgca ccaaatcaat ctagaaaaaa
     5521 tgtcgcccgt cgtccaagtc aaaaacggcg tggcgttccc cgatacctgc gtcggtactg
     5581 actcacatac gccgcacgtc gattcattgg gcgtgatttc cgtgggcgtg ggcggattgg
     5641 aagcggaaac cgtaatgctg ggacgcgcgt ccatgatgcg cctgcccgat attgtcggcg
     5701 ttgagctgac cggcaaacgc cggccgggca ttactgccac cgacatcgtg ttggcactga
     5761 ctgagttctt gcgcaaagaa cgcgtggtcg gggcatttgt cgaattcttc ggcgaaggtg
     5821 cgagaagcct gtccatcggc gaccgcgcaa ctatttccaa tatgacccct gaattcggcg
     5881 cgactgccgc gatgttcgct attgatgagc aaaccattga ttatttgaaa ctgaccggac
     5941 gcgacgacgc gcaggtgaaa ttggtggaaa cctacgccaa aaccgcaggc ttatgggcag
     6001 atgccttgaa aaccgccgtt tatccgcgcg ttttgaaatt tgatttgagc agcgtaacgc
     6061 gcaatatggc aggcccaagt aacccgcatg cccgttttgc gaccgccgat ttggcggcga
     6121 aagggctggc gaagccttac gaagagcctt cagacggcca aatgcctgac ggtgcagtga
     6181 ttattgccgc gattacttcg tgtaccaata cttccaatcc gcgcaacgtt gtcgccgctg
     6241 cgctgttggc acgcaatgcc aaccgcctcg gcttgcaacg caaaccttgg gtgaaatctt
     6301 cgtttgcccc gggttcaaaa gtagccgaaa tctatttgaa agaagcaggc ctgctgcctg
     6361 aaatggaaaa actcggcttc ggtatcgttg ccttcgcatg taccacctgt aacggcatga
     6421 gcggcgcgct cgacccgaaa atccagaaag aaatcatcga ccgcgatttg tacgccaccg
     6481 ccgtactgtc aggcaaccgc aacttcgacg gccgtattca tccgtatgcg aaacaggctt
     6541 tcctcgcttc gcccccgttg gtcgttgctt acgcgctggc aggcagcatc cgtttcgata
     6601 ttgaaaacga tgtactcggc gttgcagacg gccgcgaaat ccgcctgaaa gacatctggc
     6661 cgaccgacga agaaatcgat gccgtcgttg ctgaatatgt gaaaccgcag cagttccgcg
     6721 atgtgtatgt accgatgttc gacaccggca cagcgcaaaa agccccaagc ccgctatacg
     6781 actggcgtcc gatgtccacc tacatccgcc gcccgcctta ctgggaaggc gcgctggcag
     6841 gggaacgcac attaagaggt atgcgtccgc tggcgatttt gcccgacaac atcaccaccg
     6901 accacctctc gccgtccaat gcgattttgg ccgtcagtgc cgcaggcgag tatttggcga
     6961 aaatgggttt gcctgaagaa gacttcaact cttacgcaac ccaccgcggc gaccacttga
     7021 ccgcccaacg cgctaccttc gccaatccga aactgtttaa cgaaatggtg aaaaacgaag
     7081 acggcagcgt gcgccaaggc tcgctcgccc gcgtcgaacc cgaaggcgaa accatgcgca
     7141 tgtgggaagc catcgaaacc tatatgaacc gcaaacagcc gctcatcatc attgccggtg
     7201 cggactatgg tcaaggctca agccgcgact gggcggcgaa gggcgtgcgg ctcgcgggag
     7261 tggaagccat cgccgccgaa ggtttcgagc gcatccaccg caccaatctc atcggcatgg
     7321 gcgtcttgcc gctgcaattc aaacccggca ccaaccgcca caccctgcaa ctggacggca
     7381 cggaaaccta cgacgttatc ggcgaacgta caccgcgctg cgacctgacc ctcgtgattc
     7441 accgtaaaaa cggcgaaacc gtcgaagttc ccgttacctg ccgcctcgat actgcagaag
     7501 aagtattggt atatgaagcc ggcggcgtgt tgcaacggtt tgcacaggat tttttggaag
     7561 ggaacgcggc ttagaggtcg tctgaaaagc aagacgtagc gtgggtcggg ttcaacattt
     7621 tgctcattca cgtaattctc gatatggcag gcatctactg taaatcgtca ttcccgcgca
     7681 ggcgggaatc cagaaagtgg aattgaggaa accttattta tccgatgagt ttctgtgcgg
     7741 acaaat
//
LOCUS       DAXHSJ010000099         7667 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000099 DAXHSJ010000000
VERSION     DAXHSJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.099"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            271..1640
                     /locus_tag="ACFM8U_001434"
                     /pseudo
     CDS             271..1640
                     /locus_tag="ACFM8U_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            1884..2540
                     /gene="cmk"
                     /locus_tag="ACFM8U_001435"
     CDS             1884..2540
                     /gene="cmk"
                     /locus_tag="ACFM8U_001435"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC6334664.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            2696..4381
                     /gene="rpsA"
                     /locus_tag="ACFM8U_001436"
     CDS             2696..4381
                     /gene="rpsA"
                     /locus_tag="ACFM8U_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC6334665.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            4392..4706
                     /locus_tag="ACFM8U_001437"
     CDS             4392..4706
                     /locus_tag="ACFM8U_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC6334666.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(5057..5464)
                     /locus_tag="ACFM8U_001438"
     CDS             complement(5057..5464)
                     /locus_tag="ACFM8U_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC6334667.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            5612..6721
                     /locus_tag="ACFM8U_001439"
     CDS             5612..6721
                     /locus_tag="ACFM8U_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HFC6334668.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFELMHEGKSIRAVIHY"
     gene            6730..7557
                     /gene="fghA"
                     /locus_tag="ACFM8U_001440"
     CDS             6730..7557
                     /gene="fghA"
                     /locus_tag="ACFM8U_001440"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC6334669.1"
                     /translation="MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNLENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQSAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT         2050 a         1861 c         1836 g         1920 t
ORIGIN      
        1 ttcaaattcc ctctctttca tcattattta aatgctatca ttaatataat taacaaaatt
       61 tcatttccat acaccgatgt agaagaataa caaattaatc tatttataaa ctccgattct
      121 tcctcatcgg gttatactca tcagaaatac tcactgaaat atatgccttt tacatggaaa
      181 atcactagac agttgaaaag caatcagtca gttcctgtta aaaaacgcgt gtagaatttc
      241 cgctttatct caatcggaca cggtttcaat atgtcttccc cttcaaatac caatcgtcaa
      301 acttggtcca gccgattaac ctatatcctg accgttgccg gcgcgactgt cggtttcggc
      361 gcgacgtggc gtttcccgta tttggtcggt gaaaacggtg gtggcgcgta tgtgttttta
      421 ttctgtatcg cgatgctggt tatcggcatt ccgatgattt tggtggaaaa tgtcatcgga
      481 cggcgcaaag gcgtgaacgc gctggatgcg ttcggcggtc cgatgaacgg caaacccatt
      541 gctaaaattt ggaaactggt cggctggatg gggctgctcg gcacgttcgg catcatggct
      601 tattacatgg tactcggcgg ctgggtaatc agctatatcg ttaatattat tggaggaaat
      661 ttgaatattt ccagccccat cgacggtgtg gttacaaaag gcttctttgc cgaacacatt
      721 gaaacagccc ttgggaaatt gcgttttata cgctgctttt tgtcgccgtg aaccaatgga
      781 ttttggtcaa aggcgttatc ggcggcattg aaaaagcggc aaaatagctg atgccgctgc
      841 tgtttttgtt cctaatcgcg atggtcgtcc gcaacgttac ccttccgggc gcaatggaag
      901 gggttgcttt ctatctgaaa cctgatttca gcaagattac cgccgaactg ttcgtcttcg
      961 ttttggggca ggtatttttt gccctgagcc tgggtttcgg cgtgatgatt accttgtcca
     1021 gctatttgga taaaaacgaa aatctggtgc aaaccgcagt tatcacggca attaccaata
     1081 ccatcatcgc cgtacttgcg ggctttatga ttttcccgtc gctcttcagc ttcggcgttg
     1141 cccccgattc cggtccgact ttggtgttcc aaagcttgcc gattgtgttc tcacatatgt
     1201 gggcgggacc tgtgttcgcc gtgattttct tctctctgct cctgattgcc gctctgacaa
     1261 cttcgctgac gatttatgaa gtgttgatta cgaccattca ggaaaaaacc aaaatccgcc
     1321 gtaccgccgc aatcacgatt gtattggccg ccatctacgt tttcggcaac atcccgtcta
     1381 ttttgagcta tggcccgtgg aaagacgttt ccgtgttcgg caaaaatatt ttcgatgcct
     1441 tcgactacat cagcggcaac atcttgttta tgctgaccgc gcttggctcg gcattgtttg
     1501 tcggtttcgt gatgaaggat gaagcgaagg aagaattgct ttataaaggc aaccatacga
     1561 cggtcaatat ttggtttgcc tatgtgaaat acctcgtgcc gctggtgatt ttgctgattt
     1621 tcgtcagcaa cctgttttaa tccgcagcaa tcgatgccgt ctgaaggtca tacctctttc
     1681 agacggcatt gcattgatgt ccgccacaat acccgcaacg ccggaatccg aaccggcttg
     1741 ccggcttctg tcggatattt ccgggcaggc ggcgttccgt ctgcttagac aatcgtcctt
     1801 taaaacaggt agaatccgcc ccaacgggaa acacatcctt cagacggcaa aacccatacc
     1861 ccaaaccatc aggaatcccc attatgaaca gacaaaaagt catcgccatc gacggcccgg
     1921 gcgcatcggg caaaggcacg gtcgccgccc gcgtcgccgc cgcattggga tacgattatc
     1981 tcgataccgg cgcactctac cgcctgactg ccctatatgc acaaaaacaa ggcgtgggat
     2041 ggcacgatga agaaaacgtt tccgaactgg caaaaaaact gcccgccgta ttttcaggca
     2101 gccgcatcct gctcggcggc gaagacgttt cagacggcat ccggacagaa gccatcggca
     2161 tgggcgcatc cgcagtcgca cagttgccta aagtccgcgc cgccctgctg caacgccaac
     2221 gcgattttct gaccgaaaaa ggactggttg ccgacggacg ggataccgga tcggtcgtct
     2281 tcccccaggc cgaactcaaa atttttctga cagcagaatc caaaatcagg gccgaacgcc
     2341 gcgccaaaca aatcggcatc ccctgcgaag gtttggcatt cgagcgcatc ctgtccgaca
     2401 tcgaagccag agacgaggca gaccgaaacc gcaaagttgc ccccctgaaa caacagcccg
     2461 atgccctgct tttggacaca agccgcctga ctatagaaga aactgtaaaa aaagtgcttg
     2521 attggtatcg tgaagtttaa attttcaggt ataatcgcac aaattacgtt tcagacggca
     2581 taaaaatccc ccatatgccg tctgaaacct tatgtacccg tctgccctgc caaggacggc
     2641 agatatccac caacccaccc cgcacccctt ggcggtgtac cgaaaagagt tatatatgtc
     2701 tatggaaaat tttgctcagc tgttggaaga aagctttacc ctgcaagaaa tgaacccggg
     2761 tgaggtgatt accgctgaag tagtggcaat cgaccaaaac ttcgttaccg taaacgcagg
     2821 tctgaaatca gaatccctga ttgatgtagc tgaattcaaa aacgctcaag gcgaaattga
     2881 agttaaagtc ggcgacttcg ttaccgttac catcgaatcc gtcgaaaacg gcttcggcga
     2941 aaccaaactg tcccgcgaaa aagccaaacg cgctgccgat tggattgccc tggaagaggc
     3001 catggaaaac ggcaacatcc tgtccggcat catcaacggc aaagtcaaag gcggcctgac
     3061 cgttatgatt agcagcatcc gcgcattcct gccgggttct ttggtcgacg tacgtcctgt
     3121 aaaagacact tctcacttcg aaggtaaaga aattgaattc aaagtgatca aactggacaa
     3181 aaaacgcaac aacgttgttg tgtcccgccg cgccgttctg gaagctacct tgggtgaaga
     3241 acgcaaagcc ctgctggaaa acctgcaaga aggctccgtc atcaaaggca tcgttaaaaa
     3301 cattaccgat tacggtgcat tcgttgactt gggcggcatc gatggtctgt tgcacatcac
     3361 cgatttggca tggcggcgcg tgaaacaccc gagtgaagtc ttggaagtcg gtcaggaagt
     3421 tgaagccaaa gtattgaaat tcgaccaaga aaaacaacgc gtttccttgg gtatgaaaca
     3481 actgggcgaa gatccttgga gcggtctgac ccgccgttat cctcaaggca cccgcctgtt
     3541 cggcaaagta tccaacctga ccgactacgg cgcattcgtc gaaatcgaac aaggcatcga
     3601 aggtttggta cacgtctccg aaatggactg gaccaacaaa aacgtacacc cgagcaaagt
     3661 cgtacaactg ggcgacgaag tcgaagtcat gattttggaa atcgacgaag gccgccgccg
     3721 tatctctttg ggtatgaaac aatgccaagc caatccttgg gaagagtttg ccgccaacca
     3781 caacaaaggc gacaaaatct ccggcgcggt taaatccatt accgatttcg gcgtattcgt
     3841 cggcctgccc ggcggcatcg acggtttggt tcacctgtcc gacctgtcct ggaccgaatc
     3901 cggcgaagaa gccgtacgca aatacaaaaa aggcgaagaa gtcgaagccg tcgtattggc
     3961 aatcgacgtg gaaaaagaac gcatctcctt gggtatcaaa caactggaag gcgatccgtt
     4021 cggcaacttc atcagcgtga acgacaaagg ttctttggtt aaaggttccg tgaaatctgt
     4081 tgacgccaaa ggtgctgtta tcgccctgtc tgacgaagta gaaggctacc tgcctgcttc
     4141 cgaatttgca gccgaccgcg ttgaagattt gaccaccaaa ctgaaagaag gcgacgaagt
     4201 tgaagccgtc atcgttaccg ttgaccgcaa aaaccgcagc atcaaacttt ccgttaaagc
     4261 caaagatgcc aaagaaagcc gcgaagcact gaactccgtc aatgccgccg ccaatgcgaa
     4321 tgccggcacc accagcttgg gcgacctgct gaaagccaaa ctctccggcg aacaagaata
     4381 aggttgcaga catgacaaag tctgagttaa tggttcgttt ggcagaagtg tttgccgcca
     4441 aaaacggcac gcatcttctg gcaaaagacg tagagtacag cgtaaaagtc ttggttgaca
     4501 ccatgactag atcgcttgcc cgaggtcaac gcatcgaaat ccgcggtttc ggcagcttcg
     4561 atttgaacca tcgtcctgcc cgcatcggtc gcaatcccaa aaccggcgag cgtgtggaag
     4621 tacctgaaaa acatgtaccc cacttcaagc ccggtaaaga attgcgcgag cgggtcgact
     4681 tggctttaaa agaaaatgcc aattaaacct tagcatcaaa acgccgctgt tacgcggcgt
     4741 tttttctgtg gtttaacttc atccgctgct tcaatacctt gagccaagca agcaacggat
     4801 tagagcgtgg atttttttat agtggattaa caaaaaccag tacggcgttg cctcgcctta
     4861 gctcaaagag aacaattctc taaggtgctg aagcaccaag tgaatcggtt ccgtactatt
     4921 tacactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta tatcattgct
     4981 tacaatccgc tttttaaaca acaaattttt gatttctatt acgaacagga caaaaatcct
     5041 gcttattgca ctaaaactaa gccgtttcag gaatttgcgg caaatttaca gcttttaccg
     5101 agcctaatgc tttcgctttt tggtaaaacg ccaatttgta ttcaagcaaa tctaaatagc
     5161 gttttaattc ggcaatttga cacttcacat tttctatttg attttcaaac aaggaaaggc
     5221 gttcttcaat ggtatcgtcg ccaatgacgg tacattccgc aaagcgtttg atgtctttta
     5281 agctcattcc cgtatttttc aagcattgca ataagcccaa ccattgcaaa tcgttatcgg
     5341 taaaacagcg gttaccgtat tcatcacgtc cgatattggg caacaaacct tctttgtcgt
     5401 aaaaacgtag ggtgtgggcg gagatgccta ttttttcggc ggctttggca gtagtataag
     5461 tcattttccc tccttctaaa caaaaacagt aaaaaacact tgctttagag ttaactctaa
     5521 agtgtaaact gttgctatgt tgctcaggca aggcaacttt gtcaatgaat taagaggaaa
     5581 gacaatggaa atgaaacaag ccgattcaac catcaaatct cgtgcggcgg tggcattcgc
     5641 ccctaaccaa cccttacaaa ttgtggaaat cgacgtagaa atgccgcgta aaggcgaggt
     5701 gttaatccgc aatacccaca ctggcgtgtg ccatactgat gcgtttacgt tatcaggaag
     5761 cgatcctgaa ggcgtattcc ctgtggtgct tggacacgaa ggtgcgggtg tggtcgttgc
     5821 tgtgggcgaa ggtgtgtcaa gcgtaaaacc gggtgatcac gtgattccgc tttacaccgc
     5881 cgaatgtggc gaatgtgagt tttgttgttc aggtaaaacc aacttgtgcg tctcagtgcg
     5941 tgatacacaa ggtaaaggct taatgccgga cggcacgacg cgtttttctt atcaaggtca
     6001 gccaatctat cactatatgg gctgttcgac tttcagtgaa tactccgttg ttgccgaagt
     6061 ttcactggcg aaaatcaacc ctgaagccaa ccatgaacaa gtatgtttgc tcggctgcgg
     6121 cgttaccaca ggtattggtg cggtacataa tacggcaaaa gtgcaagaag gcgactctgt
     6181 tgccgtgttt ggcttaggcg cgattggttt ggcggtggtg caaggtgcgc gtcaagccaa
     6241 agccggccgc attatcgcca ttgataccaa tccatcaaaa tttgagttgg caaaacagtt
     6301 cggtgcaacg gattgtttga acccgaacga ttacgataaa ccgatcaaag atgtgttgtt
     6361 agacattaat aaatggggca ttgaccatac ctttgaatgt atcggcaacg taaacgtaat
     6421 gcgtcaggca ttagaaagtg cacaccgtgg ttggggtcaa tccattatca tcggcgtagc
     6481 aggtgcagga caagaaattt caacgcgtcc gttccagttg gtaacaggtc gtgtttggaa
     6541 aggctctgca tttggtggtg tgaaaggtcg ctctgaactg ccgaaaatgg tggaagattc
     6601 aatgaaaggc gacattgagt tagaaccgtt tgtaacccac acaatgacac tcgatcaaat
     6661 caatgaagcc tttgagttaa tgcacgaagg taaatcgatc cgcgccgtta ttcactacta
     6721 aggtatgcga tgaaactgat tgaacaacat caaatttttg gcggttcgca acaagtttgg
     6781 gcacacaatg cccaaacgct tcaatgcgaa atgaaatttg ccgtctattt gccaaataat
     6841 ctagaaaatc gacctcttgg cgtaatttat tggctttccg gcttgacgtg tacggaacaa
     6901 aatttcatta ccaaatcagg ctttcagcgt tatgcggcag aacatcaagt aattgtggtg
     6961 gcctccgata ccagccctcg cggagagcaa gtgccgaacg atgatgctta cgatttagga
     7021 cagagtgcag gcttttattt gaatgcgacc gaacagcctt gggcggcgaa ttatcaaatg
     7081 tatgattaca ttttgaacga gctgccccgt ctgattgaga aacactttcc taccaacggc
     7141 aaacgttcca ttatgggaca ttcaatgggc ggacacggcg cattggtatt ggcgctgcgg
     7201 aatcaggaac gttatcaaag tgtttctgcc ttttcgccta ttttatcgcc aagcctcgtg
     7261 ccgtggggag aaaaagcctt tactgcttat ttagggaaag accgtgaaaa atggcagcaa
     7321 tatgatgcta actcactcat tcaacaaggc tataaagtgc aaggtatgcg catcgatcaa
     7381 ggcttggaag atgagttttt gccgacacaa ttgcgtaccg aagattttat cgaaacctgc
     7441 cgtgcggcaa accagccggt cgatgtgcgt ttccataaag gctacgatca cagctattac
     7501 ttcatcgcca gttttattgg cgagcatatt gcttatcacg ccgcgttttt gaagtaaacc
     7561 aaagagcgtt cagtgttcaa agcagttttg ggatagccgg cacgagggcg gtaagaagtg
     7621 ccggcataaa cgtatgccgt ctgagccgga aggagccgac tctacgg
//
LOCUS       DAXHSJ010000100         7648 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000100 DAXHSJ010000000
VERSION     DAXHSJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.100"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(107..1030)
                     /gene="era"
                     /locus_tag="ACFM8U_001441"
     CDS             complement(107..1030)
                     /gene="era"
                     /locus_tag="ACFM8U_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC6334670.1"
                     /translation="MDIETFLAGERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDAV
                     VFVVEAMRFTDADRVVLKQLPKHTPVVLVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="ACFM8U_001442"
     CDS             complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="ACFM8U_001442"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC6334671.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGYANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEKKLPLKKKKK"
     gene            1963..2286
                     /locus_tag="ACFM8U_001443"
     CDS             1963..2286
                     /locus_tag="ACFM8U_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC6334672.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2334..2810
                     /gene="ribH"
                     /locus_tag="ACFM8U_001444"
     CDS             2334..2810
                     /gene="ribH"
                     /locus_tag="ACFM8U_001444"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC6334673.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2888..3313
                     /gene="nusB"
                     /locus_tag="ACFM8U_001445"
     CDS             2888..3313
                     /gene="nusB"
                     /locus_tag="ACFM8U_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC6334674.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3394..4428)
                     /gene="pyrC"
                     /locus_tag="ACFM8U_001446"
     CDS             complement(3394..4428)
                     /gene="pyrC"
                     /locus_tag="ACFM8U_001446"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC6334675.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4644..4790)
                     /locus_tag="ACFM8U_001447"
     CDS             complement(4644..4790)
                     /locus_tag="ACFM8U_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334676.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            complement(4791..5015)
                     /locus_tag="ACFM8U_001448"
     CDS             complement(4791..5015)
                     /locus_tag="ACFM8U_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC6334677.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVKHK"
     gene            5124..5735
                     /locus_tag="ACFM8U_001449"
     CDS             5124..5735
                     /locus_tag="ACFM8U_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC6334678.1"
                     /translation="MRRTTLLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            complement(5795..6667)
                     /gene="accD"
                     /locus_tag="ACFM8U_001450"
     CDS             complement(5795..6667)
                     /gene="accD"
                     /locus_tag="ACFM8U_001450"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC6334679.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPIDPLKFKDSKKYPDRLGAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(6704..7489)
                     /gene="trpA"
                     /locus_tag="ACFM8U_001451"
     CDS             complement(6704..7489)
                     /gene="trpA"
                     /locus_tag="ACFM8U_001451"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC6334680.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
BASE COUNT         1835 a         2340 c         1835 g         1638 t
ORIGIN      
        1 tgcacagata atatcaaccc gtttttaaca aagatattcc cgattgaaac aaaatgccgt
       61 ctgaaacctg ttcggacggc atttgcgtaa agttcagcaa gaaaaactac aaacccagct
      121 cgcgcaggaa gcggatgtcg tccgcccaac cggatttgac tttgacccac actttcaaaa
      181 acactttggt atcaaacagt ttttccatat ccaaccgcgc ttcggtggaa attttcttca
      241 aacgctcccc gcctttgccg attaaaatcg ccttttggct ttccttatcg accaaaacgg
      301 cgatatagat gcggtttaaa ccgtcttcct cttcaaactg ctccacttcg acgttcatcg
      361 cataaggcaa ttcctcgccc aaatagcgga acaatttttc acgcacgatt tccatcgcta
      421 aaaaacgcgc cgatttgtcc gtaaccatgt cttcgggata catcggcacg ctttcgggca
      481 gatacggttt aatcagctcc aacaggttgg caatccgcaa tccgtgtttc gcgctgactg
      541 cctccgctgc cgcaaattca aattcggcgc gcacctgggc aacaaacgcc tccaacgcgt
      601 aacggtcttt cgccttgtcc ttgtcaattt tgttgaccac taaaacgacc ggcgtgtgct
      661 tgggcagttg tttcaacacg actcggtcgg catcggtaaa gcgcatcgcc tccacgacga
      721 aaaccacggc atccacaccg ccgagtgcct cggtaacgtt ttgattcaaa cggtcgttga
      781 gcgcgttgcg gtggtcggtt tgaaaaccgg gcgtatcgac aaacacaaac tgcgcggtat
      841 cgtcggtata aatccccgtt acgcggttgc gcgtcgtctg cgcctttttg ctggtgatgc
      901 tgattttctg accgatgaga tggttcatca gcgttgattt gcccacgttc ggacgaccga
      961 caatcgccac gaagccgcaa cggtatccgt cggcggcacg ttcccctgca aggaaggttt
     1021 caatatccat attcgctttc atatccatat ttcagacggc atattcacgc gccgcctcat
     1081 ttctttttct tcttcagcgg cagcttcttt tccagccatt tcaaagcctc tttcgccgct
     1141 tcctgctccg ccgccttgcg gctcgtccct ttggcacggc acacgaaacc cagttcgccc
     1201 aaatcgcagg aaatgacaaa catactgtcg ttggcataac cgatttgctc ttcgatacgg
     1261 tatttcggca aggcgaaacg gcgcgcctgc aacgcctcct gcaaagcagt tttgccgtct
     1321 tttgcctgat tttgaaaatc ggcgcgccgg acgcgatcgg caaacaaatg gcgcaccacc
     1381 ttttccgccg tgttgaaatc ggcatcgaag ctgacggcag caaacatcgc ctccatcgcg
     1441 tctgccagta tcgaaggccg tctgaagccg ccgctcttca actcgcccgc ccccaagtac
     1501 aggccgtcgc cgacattcat ttccgccgcc atttccgcca gcacgccctc attgaccaga
     1561 cttgcccgca accgcgacaa ctcgccctcg gtcaacttcg gaaacgcgtc aaacagcatc
     1621 cgcgccaccg tataattcaa aatcgaatca ccgacaaact cgaaccgctc gttgtgcttc
     1681 gcatgatggc tcctgtgcgt caaagcctgc cgcaaaagcg aaatatcgcg gaacgcgtag
     1741 cccagtttct tctgtatcgc cgcgtgtgcc tgctgtttca aaacatcgtc tttcattacc
     1801 gtcttccgca aaaaacagcc cgaatgcgtt tccatactcc aaaaatacag aaacacctta
     1861 aggctgcaaa cggcgcacat tgtaccacca aaccgccgcc gtctgccgac aacgcacgaa
     1921 tcgtggtaaa atccgtaagt tttcccccaa cgtattcccg taatgaacgc atttgcacaa
     1981 gcactttcct cggcactcga ccgctgtatc gccaccaaca ccgtcgccaa acaaaaccgg
     2041 cccgtcggct tcctctaccg cgaagccccc gtttttgaaa acgacagcgg ctggcgtttc
     2101 ttcagcggcg acgagaccga cgaatacacc gacgatccgg acaacttcag catcgtcagc
     2161 ctcgccgaca tcgccaaaac caaccctgaa accgccgcac tgctttccca acccgaaggc
     2221 agcgcatggg agcttgccga agacggcaca ttccaaaccg tcgccgactg gcagccgcaa
     2281 gactgaaacc ttcagacggc attccgaaac ctccgaatac caaaggacac ccaatgaaca
     2341 ccatcgcccc caacctcgac ggcaaacacc tccgcatcgg catcgtacag gcacgcttca
     2401 ccaacgaaat cggcagcgaa atgctcaaag tctgctgccg caccctccaa gaattgggcg
     2461 tggcagacga aaacattacc gtcgccaccg tacccggcgc gcttgaaatt cccatcgcgc
     2521 tgatgaactt tgcctcttcc gaaaagtttg acgcactgat tgccatcggc gtcgtcatcc
     2581 gtggcgaaac ctaccatttc gagctggttt ccaacgaatc cggagcaggc gtcagccgcg
     2641 tcgcactcga ctacaatatc ccgattgcca atgccgtcct aaccaccgaa aacgacgcgc
     2701 aggcaatcga acggattgaa gaaaaagcct cggatgccgc caaagtcgcc gtagaatgcg
     2761 ccaacctcgt caaccttctg ctcgaagaac agtttgaaga cgaagaataa caaacagggc
     2821 gtacaccgat gcgccctttc cgtttcagac ggcataccgt gccgcccaac cgtttcaagg
     2881 aaaaattatg aaaacagccc gccgccgttc ccgcgagctt gccgtacaag ccgtttacca
     2941 atcccttatc aaccgcaccg ccgcgcccga aattgctaaa aacatccgcg aaatgcccga
     3001 ctttgccaaa gcggacgaag aattgttcaa caaacttttc ttcggtacgc aaaccaacgc
     3061 agcggactac atccaaaaaa tccgccccct gctcgaccgc gacgaaaaag acctcaatcc
     3121 catcgaacgc gccgtcctgc tgaccgcctg ccacgagctg tccgccatgc ccgaaacgcc
     3181 ctaccccgtc attatcaacg aagccatcga agttaccaaa accttcggcg gcacggacgg
     3241 gcacaaattc gtcaacggca tcctcgacaa actcgctgcc caaatccgcc ccgacgagcc
     3301 taaacgccgt tgacactccg gaaaatccga attggaaaat acataaatgc cgtctgaaaa
     3361 acctttcaga cggcattttg cttgaccgtt caatcaatac tgtaccgtcc agccgatttc
     3421 gccgcccgcg cgcatcggga caagttcgcc gtcgccatag ggaacacttg cggggacggt
     3481 ttggctttgt ttgacgaggg tgatcgtgcc ggcgttttcc ggaatgccgt aaaaccttgc
     3541 gccatttttc gaggcaaagg cttcgagttt gtccaacgcg cctgcttttt caaacacttc
     3601 ggcgtaaagc tcgacggcgg tcatcgcgct gaacataccg gcgcagccgc aggcgttttc
     3661 tttggcggac ttggcgtgcg gcgcggagtc ggtgccgagg aagaatttgt gcgccttctc
     3721 gccggtaacg gcggcaacca atgcctgacg gtgggtttcg cgtttgagta cgggcaggca
     3781 gaaatgatgg gggcgcacgc cgccgaccaa gaggtcgttg cggttgagca ggaggtgttg
     3841 cggggtaacg gtggcggcta cgttgtcgcc tgcttccaaa accaggcggg cggcttcggc
     3901 ggtggtgatg tgttcgaaca cgactttaag attcggcact tgcgccaaaa cgggtttcat
     3961 cacgcgcccg ataaaggcgg cttcgcggtc gaagatgtcg atttcggggt cggttacttc
     4021 gccgtgaacg aggaacaaaa tgccctgttt cgccatttct tccaacacgg ggatgagctt
     4081 gaacaggtcg gttacgccgg aatcggaatt ggtggtcgcg ccggcagggt agagtttgaa
     4141 ggcgacgatg ccggcggctt tggcttcgcg tacaagttcg ggcgtggcgt tatcagtcaa
     4201 ataaagcgtc atcaacggct caaacgcgct gccttcgggc aacgccgcca taatgcgcgc
     4261 tttgtaggca agcgcgtcgg ctacactgac gacaggcggt ttgaggttgg gcataatgac
     4321 ggcgcgcccc atttggcggg cggtataagg ggcaacggct ttgagcgcgt cgccgtcgcg
     4381 caggtgcagg tgcatatcgt cggggcggat aatggtcagg gtttgcatgg ttgttccttt
     4441 ttaggttttg gctgcgtttg aatacccggt cgattccatc cgtttgccgg gggaaagccg
     4501 gttattccgt ccggtcggaa accggttcca ttggatgaaa ggcattttgt ccgactgatt
     4561 gggttgcatc aacggaatct tcaaacacag cctttagatt ttgattgtgc ttgggttttg
     4621 cttctttgcc gacggcaagg cggttaacag ccattccgga acaaatcggc ggcgatacgc
     4681 ccgaaatgcc gacactgcac gccggacggt ttgtttttga aacggcacgc ggcacatacg
     4741 atattcaaaa acaacgccgt cgggcgggac atccgcattt cagacggcat ttatttgtgc
     4801 ttgacgacca gcgtgaacac gccgtcctgt tcggaagcat ccaacagcac atgaccggtt
     4861 tggcggcaaa aagcctcaaa atcccccggt gcgccgccgt cggttgccag aacggtcaac
     4921 acgtcgccct gctgcatttg cgccaaagcc tttttggcac gcaaaatagg caaagggcat
     4981 ttcagcccgg ttacgtctaa agtttcgctg ttcatcgttt gtaccgttta aaaaatggga
     5041 atcgggggga gattataccc cagttgcttg cagccccctt tgccaaccca taaaataacg
     5101 ccgttttcaa tttggagaaa cttatgcgcc gcaccaccct cctgctgctc gcactcgccg
     5161 ctggcacgtc ccttgctgcc ggcttcagcc aaaaagacac gccgatcaac acccgctacc
     5221 gcgaaacgcc cgaagaagcc gccgcccgcg aatttaaaga acataccgcc aaactgccgc
     5281 cgctgcccga tacgcactcg gacggctggt tcgacattta tgtggatgaa aattacggca
     5341 aacagccgaa aatcctgctc gacagcctgc aaattatgcc cgcgcccgac ggcagcatcc
     5401 gctacattct caatatccgc tccgacaaag gctacgacaa cctgactgcc gaaggcatat
     5461 tctgcgcccg cttctccatc aggttcggca acggcaaacc ttcctcctat aaagtgttcg
     5521 gctacggcga cacggtcaac cgccgctgga tacagccccg aaacgccgaa tggaaaccga
     5581 taggcggtac attaagccgc aacgacgcac tgcgcaccgt cctgtatcag gcattctgcg
     5641 agggcggtgt acccgccgac acgaaaggtt tggtacaacg cctgaaagaa cgtgccggac
     5701 gctatgcccc gtcgataaag ccgcacaaca aataaccaga cacaaaaata ccgtctgaaa
     5761 catcggtttc agacggtatt cgattcatca gccatcaagc ggcggaaact ttgtcctgac
     5821 ggcacaacag cgtaatcaaa tcactgatgc gccgcttcat atcgcggcgg tcgacaatct
     5881 ggtcgattgc gcctttttcc agcaggaact cggcgcgttg gaagccttcc ggcagcgttt
     5941 cgcgcaccgt ctgctcaatc acgcgcggac cggcaaaacc gatcagcgcg ttcggttcgg
     6001 caagcacgac atcgcccaaa aatgcgaagc tggcggatac gccgcccata gtcggatcgg
     6061 tcaacaccga tataaatggc aggcgttttt ccgtcagcaa atgcagcgcg gcactggttt
     6121 tcgtcatctg catcaatgag ttcacaccct cctgcatacg cgcaccgccg gaagccgcca
     6181 cacagacgaa cggacaattg tcggcaaccg cccgacggat accttgtacg aatcgttcgc
     6241 ccacaaccga acccatcgaa ccgccgataa agcggaattc aaacgcagca acgacgacgg
     6301 gcaggccgtt catcatcccc ttcatcacca ccagcgcgtc atcttccccg gtcagcttgc
     6361 gtgccgcccc caaacggtcg ggatattttt tactgtcttt aaacttcaaa ggatctatcg
     6421 gtttgacatt tccggcaact tcctccctgc catcctcatc caaaagcagg ttcaggcgtt
     6481 gtcgtgccga caacggattg tggtggttgc atttcggaca aacctgattg ttctgctgca
     6541 actcggtcga ataaacggtt gccgaacaag acggacattt gtgccacaaa ccctcgggaa
     6601 cattggaaga accgtctttg ctgcggtttt tgattttggg tggcaggatt ttatctaacc
     6661 agctcatgga caactcctaa aatatttaga atgcgtgaca gaatcagcgc acggcatcct
     6721 tcaactcttt gaccaaagca ccgacgaccg cagcctcgtt gcccgcattg ttttcaatct
     6781 ctttcacaat ccggctgccg acaatgaccg cgtcggcaac ccggccgatt ttgcgcgcgc
     6841 tttccgcatt gctgatgccg aaaccgacac cgatgggaat atcgatatac tgatgcaaat
     6901 actctatttt acgcgaaacc tcatccgtat ccaaacttgc cgcgcccgtt acgcccttga
     6961 gcgaaacata atagacaaat ccgcctgcca gctcggcaat ggttttaata cggtcttccg
     7021 tcgtcgtcgg cgcaatcagg aaaatacagt cgaccccgtt atccttcagc tcgcgataga
     7081 gcggatcgat ggtttcgaca ggggaatcca ccgtcaacac gccgtccaca cccgcctttg
     7141 cggcttcctg agcaaactcc cgataaccca tcttatgtac agggttcaaa tatcccatca
     7201 aaacaaccgg cgtttgcgtg tcggtttcac ggaattttct gacgacatcc aagacatcgc
     7261 gcagcgaaat cccgtttgcc aacgcccgct ccgccgcacg ctgaataacc ggcccatccg
     7321 ccatcggatc ggaaaacggc acacccaact ccaaaatatc cgcaccgttt gcaaccatgc
     7381 cgtgcatcaa tgccaaagtt gtccgaatat cggggtcgcc caccgtaata tagggaatca
     7441 atgcctttcc gccatccaaa gcggcaaaag cctgccggat tctgctcatt tgtctcttcc
     7501 atcctatcgg caaatatgcc gtaaaacaca atcagtaaat ttttcccagt ataacatttg
     7561 cggtacggat aggcgaatac ggcaaacaga aaccgcgcaa atcagagccg ccgtttgaac
     7621 gaggcggaaa taaacggcaa gcatctcg
//
LOCUS       DAXHSJ010000101         7547 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000101 DAXHSJ010000000
VERSION     DAXHSJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.101"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..419
                     /locus_tag="ACFM8U_001452"
     CDS             <1..419
                     /locus_tag="ACFM8U_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC6334681.1"
                     /translation="EAEWAGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKA
                     AAAGHGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHG
                     NLGWLLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="ACFM8U_001453"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="ACFM8U_001453"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC6334682.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1376..1723
                     /locus_tag="ACFM8U_001454"
     CDS             1376..1723
                     /locus_tag="ACFM8U_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC6334683.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="ACFM8U_001455"
     CDS             1728..2321
                     /locus_tag="ACFM8U_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC6334684.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPYPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="ACFM8U_001456"
     CDS             2385..2993
                     /locus_tag="ACFM8U_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC6334685.1"
                     /translation="MKLKLHTVRILTAAIFSLALSGCVSAVIGGAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASQPRSLGDITNDTWNTSKIRATLLGISPTTQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3651
                     /locus_tag="ACFM8U_001457"
     CDS             3001..3651
                     /locus_tag="ACFM8U_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334686.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3691..4470
                     /gene="map"
                     /locus_tag="ACFM8U_001458"
     CDS             3691..4470
                     /gene="map"
                     /locus_tag="ACFM8U_001458"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC6334687.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4625..4924
                     /locus_tag="ACFM8U_001459"
     CDS             4625..4924
                     /locus_tag="ACFM8U_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6334688.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5162..5536
                     /locus_tag="ACFM8U_001460"
     CDS             5162..5536
                     /locus_tag="ACFM8U_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6334689.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5723..7189)
                     /locus_tag="ACFM8U_001461"
     CDS             complement(5723..7189)
                     /locus_tag="ACFM8U_001461"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC6334690.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1951 a         2286 c         1840 g         1470 t
ORIGIN      
        1 aagaggcgga atgggcgggc agcctgttcg gcctgcacaa atctttcggc ttcctcacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cagttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgccgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaggtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ctatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 attgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcgccgcgcg attggttgcc
      661 gtcggcagac aatgccgtct gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt
      721 tttcgtaatt tcgcgcgcca gcattaatcg gcgttcgggg aacagttccg ccatatcggc
      781 aagcgtcgca ccgatgcggt gcggcgtttc aaacatcacg acgggaaacg ccgcccgcac
      841 ccatttggca aacagtttcc tgcgctctcc cgatttcggc ggtacaaaac cgttgaaata
      901 aaaatcggat cccgccacac cggccacact caaagccgcc atcaccgcgc ttgcgccgac
      961 aacagggaca actttaaacc cgacctcacg cacgcggcgg gcgagtttcg cgcccgggtc
     1021 gcacacggcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagata
     1081 gccgacaatc ttgtccgcca tctgccgttc gttgtgttcg cgcacgctga caagtttgcc
     1141 ctgaatgccg tacgcgctca aaagctgcgc ggtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcgcg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgtcg gaggctttct gcaagtgttt
     1321 ctgaaacata aaaaatgccg tctgaaaaac agacattata aaggttaaac tgattatgcg
     1381 cctaaaccac aaacagggcg aggcaggcga agatgccgcg cttgccttcc tccaatccca
     1441 aggctgcaca ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgtcgca tacagcattt ccccatccaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa cagaacaggt tgacaaacgt accgtgccgc ctcgacgcgg tactcatcga
     1681 aggcagccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccgcccat tttgccgaaa gcatccgtgc caagcaagaa gccgaaaaag
     1801 tactggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaatgacg
     1861 gcaaaatcct ggcgtgcggc aacggcggtt cggctgccga cgcacagcac ttcgccgccg
     1921 aaatgaccgg gcgttttgaa aaagaacgta tggaactcgc cgccgtcgcg ctgaccaccg
     1981 acacctccgc gctgaccgcc atcggcaatg actacggctt cgaccacgtc ttcagcaaac
     2041 aagtacgcgc cctcggacgc gccggcgacg ttctcgtcgg catttccact tccggcaatt
     2101 ccgccaacgt catcgaagcc gtcaaagccg cacacgaacg cgatatgcac gtcatcgccc
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatactcaa agacaccgac gttttactca
     2221 acgttcccta tcctcgaact gcccgtattc aggaaaacca catcctgctg atacacgcca
     2281 tgtgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagcc cccgatgaaa ctcaaactgc
     2401 acaccgtccg catcctgacc gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg cggtgcggcg gtcggcgcga aatccgccgt cgaccgccgc accaccggcg
     2521 cgcaaaccga cgacaacgta atggcgttgc gtattgaaac caccgcccgc tcctacctac
     2581 gccaaaacaa ccaaaccaag ggctacacgc cccaaatctc cgtcgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgaaaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtgt acaactacat taccgtcgcc tcgcaaccgc
     2761 gctccctcgg cgacatcacc aacgacacct ggaatacctc caaaatccgc gccacgctgc
     2821 tgggcatcag ccccaccacg caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc caaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgtcgtattg
     3121 ctcatctgta tcaccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcggca aaaccctcca aaccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcaacctg ccgacacagc tcaaaccgac
     3421 aggcagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cccacaaacg tcccccgtcc cgaaccccga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacac ccaaagaaaa agaaacgccc aaagaaaacc ataccaaacc ggacaccccg
     3601 aaaaacacgc cgcccaaacc ccataaagaa attctcgaca acctcttctg acccggcacg
     3661 gcaggcacac ccgcaatcca aggaagcatt atgaacggca tcatcatcaa aacccccgaa
     3721 gaaatcgaaa aaatgcgcga gctgggcaaa ctcgtcgccg aagccctcga ctacatcgga
     3781 caattcgtca aacccggcgt aaccaccgac gaaatcgaca aactcgttta cgactaccac
     3841 gtcaacgtcc aaggcggcta tcccgccccc ctgcactacg gcaacccgcc ctaccccaaa
     3901 tcctgctgca cctccgtcaa ccacgtcatc tgccacggca ttcccgacga caagccgctc
     3961 aaagaaggcg acattatcaa catcgacctc accatcaaaa aagacggctt ccacggcgac
     4021 tccagccgta tgtttaccgt cggcaaagtc tcccccatcg cccaacgcct gatcgacgta
     4081 acccacgcct ccatgatggc gggcatagaa gccgtcaaac ccggcgcgac actgggcgac
     4141 gtaggttacg cctgccaaca ggttgccgaa aacgccggct attccgtcgt acaggaattc
     4201 tgcggacacg gcatcgggcg cggtttccac gaagccccgc aagtgttgca ctacggaaaa
     4261 aaaggacagg gccccgttct aaaaccgggt atgattttta ccgtcgaacc gatgatcaac
     4321 caaggcaaac gccacctgcg tatcctcaac gacggctgga cggtggttac caaagaccgc
     4381 tccctctccg cccaatggga acacgaagtc ttggtgaccg aaaccggcta cgaaatcctc
     4441 accgtcagcc ccgcctccgg caaaccctga aaccggacgt atccgcccca taaaaacaaa
     4501 caatgccgtc tgaaagaaac ggcagatatg atatataata taaaaacagg cttgacccgg
     4561 cacattacga aaacaaagca aatcggaatt tgccccgcaa ccagacaaac ttaaaggaag
     4621 ttttatgaaa atatttgaaa atatagaaga tgttaaagcc atccgtaaaa agaccgggct
     4681 gaaccagata gacttctggg gcaaggtcgg cgttacccag tctggaggct cgcgctacga
     4741 aaccggccgc aaaatgccca aacccgtacg cgaactgctc cgcctcgtcc atatcgaatg
     4801 catcgatttg gcgaaagtca acaaaaaaga tatggaaatc gccgccctgt tgaaaaaaca
     4861 ccatcccgac ctgtatgccg aattgtccaa acagaccaag tccgaaagaa aaaaacaaag
     4921 ttaaaccgca acctccggat gcccgaaggt ttttcatttc cgaaaaacgc aaacaatgcc
     4981 gtctgaaaca ccggacaggc cgccgtatcc cgcctgccgc ccctgcctca aaccgccgaa
     5041 ccgcccgaac ccgccttttt acaaacttta tccaatttcc tgtttatttc aggatacgcc
     5101 gacattagaa tgtcaaacag ctcgaaacgg gcaaactcca catccatcca aaggaataaa
     5161 aatgaaactt ctgaccaccg caatcctgtc ttccgcaatc gcgctcagca gtatggctgc
     5221 tgctgccggc acgaacaacc ccaccgttgc caaaaaaacc gtcagctacg tctgccagca
     5281 aggtaaaaaa gtcaaagtaa cctacggctt caacaaacag ggcctgacca catacgcttc
     5341 cgccgtcatc aacggcaaac gcgtgcaaat gcctgtcaat ttggacaaat ccgacaatgt
     5401 ggaaacattc tacggcaaag aaggcggtta tgttttgggt accggcgtga tggatggtaa
     5461 atcctaccgc aaacagccca ttatgattac cgcacctgac aaccaaatcg tcttcaaaga
     5521 ctgttcccca cgttaatcag gcaacaaaaa acagcgtttt cagaaatgaa aacgctgttt
     5581 tttgactgtt ccattattca caaaagggaa aaaacgatta cctgccccgt gtaccaaaac
     5641 ctgccctgcc ggatgaaggg cataaccggc agggacggca tcaacaccat atgggggtac
     5701 ggcttttctt gaaagattcg gcttaaatat ccaacacttt cgcggtatag gcgataattt
     5761 catccgccct ttcagggttt tcgttcaact tgatgccgta acccggtacc agctctttca
     5821 gacgaccttc ccaagacggg gcgcgctcgg ggaagcattg gtgcatcagc cggatcatca
     5881 gcggcacagc ggtcgatgcg cccggcgacg cgcccagcaa tgcggcgagt gagccgtcgg
     5941 cgtgggcgac aatctccgta ccaaactgga gcacgccgcc tttttcggag tcttttttaa
     6001 tgatttggac gcgttgccct gcggtgatga gttcccagtc gtcggggttt gcctcggggt
     6061 agtattccag cagggaggcg aagcgttctt ctttggtttt acgcaattcg cccagcaggt
     6121 atttggtcag cggcatattc gcccagccgg cgcgcagcat aggatagaga ttgtccatat
     6181 ggatggacag cggcaaatcc ataagcgagc cttgcttgag gaagttggaa cggaagcctg
     6241 cgtaagggcc gaacataagg tggcgtttgc cgtccacgtt gcgtgtgtcg aggtgcggga
     6301 cggacatcgg cggcgcgccg acggaagcct gcccgtacac tttggcgttg tgttgttcgg
     6361 cggtttcggg gttgctgttg cggaagaaca ggccggacac ggggaagccg ccgtagcctt
     6421 tgccttcggg gatgccggat ttttgcagca gggtcagcgc gccgccgccc gcgccgagga
     6481 agaggaagcg ggtacggagg gtgagctgcc cgtcggggtt gcgggtatcg gcggttttga
     6541 gcacccacgc gccgtcggat tcgcgtttga tgtcttcgac gtggcggttg aactcggttt
     6601 ttacgccctt gccctgcaaa tatttcacca tttggcgcgt cagccgtccg aaatcgacat
     6661 ccgtaccttc ggcggagtag ttggcggcga cgggttggtt ttcgtcccgg ccgcgcatca
     6721 tcagcggagc ccaatcggaa attttgttcc gatcggtgga aaattccata ttttcaaaaa
     6781 gtttttgggt tttaaacgcg tcataacgtt tttgaagata agaacaatgg tcttcattca
     6841 tcaccaaaga catatgcggc acggcattga tgaaggaatt gtcttccaac ttgccttccg
     6901 cgaccagcgt cgcccaaaac tggcggctga catgaaactg ttcggcaata ttgagggcgc
     6961 gcgccggatc gataatccca tttgcaccca acggcgcata gttcaattca cacagcgcgg
     7021 aatgccccgt gccggcgttg ttccacgcgt ttgacgattc caacgccaca tcttccaagc
     7081 gttcaatcag ggtgatttcc caagacggtt cgagttcttt gagcaaaacg cccaaagtcg
     7141 cgctcataat gccgccgccc accaagacaa cgtctgtcgc ttcagccatg gtttactcct
     7201 aaaaaacagg catcttctgc ccttatggtt atttgccgta ctacaaacgc ctgaatcgca
     7261 aaagcagggg aaaccggcaa tggtgtgtgt ccgagtatgc tgtttcgggg ttggaatgcg
     7321 ttgcaagcat ggcttccgac accgcttcag gggcttgtaa tatgttatcg tgaatgtagt
     7381 ggattttact gggaaatgca aagtttttct gtcgcccgcc aagtcgggaa actgcgaaat
     7441 gaaaaataaa aatagttatt tatctatata tatcaaattt ttaatagata aaaaatcaaa
     7501 attgtttata tattaatttt taaaagattg tcagcatatt gcgttaa
//
LOCUS       DAXHSJ010000102         7546 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000102 DAXHSJ010000000
VERSION     DAXHSJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.102"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            140..721
                     /locus_tag="ACFM8U_001462"
     CDS             140..721
                     /locus_tag="ACFM8U_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6334691.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(783..1637)
                     /gene="folD"
                     /locus_tag="ACFM8U_001463"
     CDS             complement(783..1637)
                     /gene="folD"
                     /locus_tag="ACFM8U_001463"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC6334692.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1860..1937
                     /locus_tag="ACFM8U_001464"
     tRNA            1860..1937
                     /locus_tag="ACFM8U_001464"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1895..1897,aa:Pro,seq:tgg)
     gene            1962..2038
                     /locus_tag="ACFM8U_001465"
     tRNA            1962..2038
                     /locus_tag="ACFM8U_001465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1996..1998,aa:Arg,seq:tct)
     gene            2066..2141
                     /locus_tag="ACFM8U_001466"
     tRNA            2066..2141
                     /locus_tag="ACFM8U_001466"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2099..2101,aa:His,seq:gtg)
     gene            2352..2858
                     /gene="rfaE2"
                     /locus_tag="ACFM8U_001467"
     CDS             2352..2858
                     /gene="rfaE2"
                     /locus_tag="ACFM8U_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFC6334693.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2855..4633
                     /locus_tag="ACFM8U_001468"
     CDS             2855..4633
                     /locus_tag="ACFM8U_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC6334694.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLHELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVSGVDEKGILHLDTGDGRQTVVSGEISLRPDDRPVSVPKK
                     REPERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4746..5570
                     /locus_tag="ACFM8U_001469"
     CDS             4746..5570
                     /locus_tag="ACFM8U_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC6334695.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAQAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADAEEIRKITSLYGKLNLKSCK"
     gene            complement(6333..7121)
                     /locus_tag="ACFM8U_001470"
     CDS             complement(6333..7121)
                     /locus_tag="ACFM8U_001470"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC6334696.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     AGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(7335..7529)
                     /gene="thiS"
                     /locus_tag="ACFM8U_001471"
     CDS             complement(7335..7529)
                     /gene="thiS"
                     /locus_tag="ACFM8U_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC6334697.1"
                     /translation="MNIILNGEPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         1611 a         1948 c         2252 g         1735 t
ORIGIN      
        1 atgtatggcg taaaggaagc gttttaacgc cgtattggga aaatgtaaag ttctttatcg
       61 ggatttcttt aagtattggc gttttcggtg tcgtggcggt agaatcgggg tcagttaaga
      121 acaattatca ggaacggata tggaagaaaa aaacgactac accgacgcgg catccgacaa
      181 ccgcaacggg caggaaattg aagtcggaat tgccggcgca ggcgaccgga tacttgccgc
      241 gctgctgaac caattgttta cctttttggt tttgttggtg ccgtttgccg gactgattgc
      301 ttttgccatc aagaatgaag gcaggattgg cggcagtgag gaaatattcg ggctgctttt
      361 gggcatgact tccttttggg taggactggc gggcattttg gtttataccg tcgttcaaat
      421 ctactatatg agtcgggacg ggcagtcatt gggtaagaaa atcatgagaa tccgcgtgtt
      481 gaaaaccgac ggccgcaatc ccggttttgt cggcacggtt ttggtacgcg aaatcgcatg
      541 gtcggttttg gttgccatta ttgccgccgt tatcggtctt gcagtaggtg acaacggaga
      601 aaacgccatc aacctgctgg cattccttgc caactttgtc ctgctcttta tggtcaaacg
      661 cgaccgccgc acgctttacg acatactggc ggatacggtt gtcgtcaagc tgcccaaata
      721 aacggacacg gcaaaatgcc gtctgaaagc ctttcagacg gcatttttat cttcagaacc
      781 gctcaagcat cgtgcagtga agccgcgtgc agggtgtttt ccatcaatgt ggcaatcgtc
      841 atcggaccca cgccgccggg aacgggcgta atcatcgccg cccgttcttt tgccgtttca
      901 aattccacgt cgccgcacag gctgccgtcg tccaaacggt tgatgcccac atcgataacg
      961 accgcgccgg gtttgatcca ttcgcctttg acaaagttcg gaatgcccac gccgaccacc
     1021 aaaatatcgg ctccggcaac ctcgtctgtc agattttcgg ttgcgctgtg gcagaccgtt
     1081 accgttgcgc gcgccagcag cagttccaaa gcctgcgggc ggccgacgat attcgacgcg
     1141 ccgaccacga ccgctttttt ccccttcgga tcaatgccgt aagcttccaa aagcgtcatc
     1201 acgcccttgg gcgtacacgg gcgcatcagc ggcattttga ccgccagcct gccgacattg
     1261 taaggatgga agccgtccac gtccttatcc ggcgaaatac gttccaaaac cgcctggctg
     1321 tcgaggtgct tcggcagcgg tagctgaacc agaataccgt ccacttcgga atcggcattc
     1381 aggcggtcga ccagtgccag cagttcttcc tgcgatgttg attcgggcag ctcgtaagac
     1441 agtgatttga tgccgcattt ttggcaggca gttttcttgt tgcggacata aaccgcgctg
     1501 gcagggtcgc ctccgaccaa aaccacggcc aggcaaggca tgtgcagatt gtcctgttgg
     1561 cgttgcgcca ccgcttcggc aaccgcctgc aggcgttttt gcgaaacttc tttaccattg
     1621 atcagttgtg ccgacatatc cgtccttttg ttaaacaggt agaaaatgct gaaaattatc
     1681 gcattttccg tttgttttta cagcaggatg ccgaaaataa atttgccctg aaataaaata
     1741 cggctgtttg gacggattaa atattttttc aaatcaaaaa actatatttt tcatcttggt
     1801 gtcttgaaaa taaatattga cgcatctttc cctgagttgt atagttcgct tcttcaagtc
     1861 ggggcgtagc gcagcccggt tagcgcatct gctttgggag cagagggtcg tgagttcgaa
     1921 tcccaccgcc ccgaccaatc aatcagcacg aatcggcatg agcgcccgta gctcaaccgg
     1981 atagagcacc gaccttctaa gtcgggggtt acaggttcga ttcctgtcgg gcgtgccaag
     2041 aatttgattg aaaaccgttt ttatggtggc tgtagctcag ttggtagagc cccggattgt
     2101 gattccggtt gtcgtgggtt cgagccccat cagccacccc agataaaggc ccgtactttt
     2161 cggtacgggt ctttttccgt ttgcgcgtac tgtccgcgcc gtcgtaccgg tgcggcggta
     2221 tgccgtctga aagccgggcc ggtcatacgg aaaccgcttc agacggcatt tgcgccgccg
     2281 gctttgcctt tcgcatatgc ttctgtatca tgtaggtttt ctgtctttta tggtttttgt
     2341 cggagacttt gatggttgac gcttggtctg ttcctgattt tgaatcgaaa atctgtccgc
     2401 ccgaggcgtt ggcggcgcgt ttggcgttgt tgccgcgccc gctggtgttt accaacggct
     2461 gtttcgacat cctccacagg gggcacgtta cttatctggc gcaggcgcgt tcggcagggg
     2521 cggcgttggt gctggcgttg aataccgatg cttcggtgcg gcgtttgggc aagggcggcg
     2581 accgcccgat taatcctttg gagaaccgtg ccgccgttgc cgccgcgttg gaaagtgtgg
     2641 atttggtaac gtggtttgac gaggatacgc cggcggcgtt gattgaggcg gtcaaacctg
     2701 agattttggt caagggcggc gattgggctg cggataagat tgtcggtgcg gcagaaacgt
     2761 tggcgcgcgg cggtcaggtg ttttcaattc cgtttctgca ccagacttcg acaacgaaga
     2821 ctttggcaaa aatccgtgcg gcagagggcg gaaaatgacg gttttgaagc tttcgcactg
     2881 gcgggtgttg gcggagcttg ccgacggttt gccgcaacac gtctcgcaac tggcgcgtat
     2941 ggcggatatg aagccgcagc agctcaacgg tttttggcag cagatgccgg cgcacatacg
     3001 cgggctgttg cgccaacacg acggctattg gcggctggtg cgcccattgg cggttttcga
     3061 tgccgaaggt ttgcacgagc tgggggaaag gtcgggtttt cagacggcat tgaagcacga
     3121 gtgcgcgtcc agcaacgacg agatactgga attggcgcgg attgcgccgg acaaggcgca
     3181 caaaaccata tgtgtgaccc acctgcaaag taagggcagg gggcggcagg ggcggaagtg
     3241 gtcgcaccgt ttgggcgagt gtctgatgtt cagttttggc tgggtgtttg accggccgca
     3301 atatgagctg ggttcgctgt cgcctgttgc ggcagtggcg tgtcggcgcg ccttgtcgcg
     3361 tttaggtttg gatgtgcaga ttaagtggcc caatgatttg gttgtcggac gcgacaaatt
     3421 gggcggcatt ctgattgaaa cggtcaggac gggcggcaaa acggttgccg tggtcggtat
     3481 cggcatcaat tttgtcctgc ccaaggaagt agaaaatgcc gcttccgtgc aatcgctgtt
     3541 tcagacggca tcgcggcggg gcaatgccga tgccgccgtg ctgctggaaa cgctgttggt
     3601 ggaactggac gcggtgttgt tgcaatatgc gcgggacgga tttgcgcctt ttgtggcgga
     3661 atatcaggct gccaaccgcg accacggcaa ggcggtattg ctgttgcgcg acggcgaaac
     3721 cgtgttcgaa ggcacggtca gcggtgtgga cgagaagggt atattgcatc tggataccgg
     3781 agacggcagg caaaccgtag tcagcggaga aatcagtttg cgtcccgacg acaggccggt
     3841 ctctgttccg aagaagcggg aaccggaacg ttttttgctg ttggacggcg gcaacagtcg
     3901 gctcaagtgg gcatgggtgg aaaacggcac gttcgcaacc gtcggtagcg cgccgtaccg
     3961 cgatttgtcg cctttgggcg cggagtgggc ggaaaaggcg gatggaaatg tccgcatcgt
     4021 cggttgcgct gtgtgcggag aattcaaaaa ggcacaagtg caggaacagc tcgcccgaaa
     4081 aatcgagtgg ctgccgtctt ccgcacaggc tttgggcata cgcaaccact accgccaccc
     4141 cgaagaacac ggttccgacc gctggttcaa cgccttgggc agccgccgct tcagccgcaa
     4201 cgcctgcgtc gtcgtcagtt gcggcacggc ggtaacggtt gacgcgctca ccgatgacgg
     4261 acattatctc gggggaacca tcatgcccgg tttccacctg atgaaagaat cgctcgccgt
     4321 ccgaaccgcc aacctcaacc ggcacgccgg taagcgttat cctttcccga ccacaacggg
     4381 caatgccgtc gccagcggca tgatggatgc ggtttgcggc tcggttatga tgatgcacgg
     4441 gcgtttgaaa gaaaaaaccg gggcgggcaa gcctgtcgat gtcatcatta ccggcggcgg
     4501 cgcggcaaaa gttgccgaag ccctgccgcc tgcatttttg gcggaaaata ccgtgcgcgt
     4561 ggcggacaac ctcgtcattc acgggctgct gaacctgatt gccgccgaag gcggggaatc
     4621 ggaacatact taaaaaatac accctttttc aacacggggc ggcatccgtc cgaatccgtc
     4681 cggattctgc cgcccgacag actgccgccc aggcgggcgg ggtttcaaac attgaggaaa
     4741 ttctaatgaa atggctcttc acccttttgg cggtactcaa tattgccgta ttcggcggca
     4801 cggtgggcta taaattagcc gttaaagcga cgggcggcgt gccggaaaac cgggccgtcg
     4861 aaaacacgcc ccccgcaacg cccgccgccg acaatgcggc ggcatccgtg gaggatacgg
     4921 cggcactgct caaaccgggc gacatcctga ccgaagagca ggccgagcag ttgcgcttga
     4981 aaaaggaagc ggaacagaaa aaactgaggg agaaaaaaca gcgtgaagaa aaagcccgcc
     5041 gcgaaaaact cgccgccgaa aaggcgcagg cggaacgcga aaacggcgcg gcggatgcct
     5101 tatgcgccgc gcaggcaagc ctcacgatgg acgaagatga ctaccaccgc atcaaaggac
     5161 ttttgggcaa atggtcgcac gttgccagca ggagcgtcga aaaacgcacc gcccaagcca
     5221 aacctgccga caaaacctac cgcgtcgtcc tgcccgtttc cgccgatgcc gaaaatcagg
     5281 cggcggagct gtctgccaaa ggtttcaacc ccataccgtt tgacggcgca ttgagtttgg
     5341 gtgtcggcaa cagccgggaa aacgcccaag ccctgcaaaa ccggcttgcc ggcgccggat
     5401 tcggcggggc gcatattgtc gaacactttg ccgaagccga caggcaggac gattctttga
     5461 gcgtgtcgcg tatgacggtt ttgtttaccg gcgtgaatgc cgccgatgcg gaagaaattc
     5521 gtaaaatcac gtccctatac ggcaaactga acctcaagtc ttgcaaatag gcgcgaaagc
     5581 cggacgataa acgccaaacc caccgcgaag gtgggtttgg cgtttcggtt ttgagtatct
     5641 gatatataaa aagggaatgt atgtctcgta tatgccgact ccaagtgtga aagtgaattg
     5701 tgagggaata cagtgtttaa tggttcggaa tggacgactt ccgagtggtt ttgattatac
     5761 gctaataata ataattatca ataaaaattt aaaaataaat gcggattttt tgtaatacga
     5821 tgaaaaataa acaaattaac ctgtccgccg atgtttgtga ttcgggtcgg caaactttca
     5881 ttgtgtcgga caaatcgggg tcggcagtca tagctgaggg cttcgtgcct gacggaaata
     5941 agcctgccgt gtttgaacat ccgtccgatt tggcgcggac agggttgtca ttcgggggtg
     6001 tttgagcgtg gattcgggga tgcaaaagcg gtaaaaccgt tgcggatgaa ggcttacgga
     6061 cggctttagg tatttttaag tgtattcaaa caacaacaaa acgcttcttc tattgccgta
     6121 tttagggctt gcgcggcagc ctgcacaccg tggaggccga gtgtttcgtt ggttgcggca
     6181 tcgggacggg cggcaaggtt tttcggccgg tttttccatt ttttccgaca aagccgtgat
     6241 gctggggaat ggcggtttgt ccgtttcagg ccgccgccgt tttggaccgc gatgccgtct
     6301 gaagccttca gacggcattt ttgccgcctt tttcaatatt ccgccgaatg ccaaaacggt
     6361 tgtccgactg tcggcgtgct ggcttgcgct ttgtcgcgtg cttcgaccgg tccggcttca
     6421 aatgccagcc gtccggattc gacggcgagt gcaaaagcgc gcgccatatt gaccggatcg
     6481 ccgctgcggg aaacggcggt gttcaaaagc acgccgtcaa agccccattc catcacttgt
     6541 gccgcctgtg agggcaaacc caagcccgcg tcgataatca gcggcgtgtc gggcaggcgt
     6601 tcgcgcagga cgttcaacgc gtaggcatga accgcgccca aacccgtgcc gatcggggcc
     6661 gcccacggca ttaacgcctg acagcccgcg tcgagcaggc ggcggcaggc aatcaggtct
     6721 tcggtgcaat aaggcagcac tttgaagccg tctttaatca ggatttccgc cgcttcgaca
     6781 agttggaaca catccggctg caaggtgtcg tcgtcgccga tgagttcgag tttaatccaa
     6841 tcggtttcaa acacttcgcg cgccatttgc gccgtcgtta ccgcttcctg cacactttgg
     6901 cagcctgccg tgttcggcag gacgggaacg ccggtttctt gaagcagcga ccaaaacccc
     6961 tgaccgtgcg cctcgccgcc gcatcccgcg cggcgcagcg agacggtaat catcgcgggc
     7021 cgggcggttc ggacggattg tttgaggatt tcaggggtcg ggtaggcggc tgtgccgagc
     7081 agcagccgcg aagggaaagt ttctccgtat agggtgagca taatgggttc ctttgtatag
     7141 tggattaaca aaaaccagta cggcgttgcc tcgccttagc tcaaagaaaa cgattctcta
     7201 aggtgctgaa gcactaagtg aatcggttcc gtactatccg tactgtctgc ggcttcgtcg
     7261 ccttgtcctg atttaaagtt aatccaccat aacgttgttt tggggacagg ggtcgtctga
     7321 aaaggcaaaa ccgcctagcc gccgaccacc ggccgcacga tatcgatttt gtcgttttcg
     7381 tttaaaaccg tttccgcata cgcgcctttg gggacgaaaa cggtgttgac cgccacggca
     7441 aagggctttt gcggcgcggt ttgggcgatg aggtcggcaa cgcttgtgcc gtgaagttcg
     7501 gcgggttcgc cgtttaagat gatgttcatg gtttctcgat gtttct
//
LOCUS       DAXHSJ010000103         7380 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000103 DAXHSJ010000000
VERSION     DAXHSJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.103"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(86..445)
                     /gene="rnpB"
                     /locus_tag="ACFM8U_001472"
     ncRNA           complement(86..445)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFM8U_001472"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(524..1627)
                     /gene="prfB"
                     /locus_tag="ACFM8U_001473"
     CDS             complement(524..1627)
                     /gene="prfB"
                     /locus_tag="ACFM8U_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC6334698.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFTAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1730..2623)
                     /locus_tag="ACFM8U_001474"
     CDS             complement(1730..2623)
                     /locus_tag="ACFM8U_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC6334699.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKLVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2966..3415
                     /locus_tag="ACFM8U_001475"
     CDS             2966..3415
                     /locus_tag="ACFM8U_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6334700.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3489..3797
                     /locus_tag="ACFM8U_001476"
     CDS             3489..3797
                     /locus_tag="ACFM8U_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334701.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3913..4755
                     /locus_tag="ACFM8U_001477"
     CDS             3913..4755
                     /locus_tag="ACFM8U_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC6334702.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEC
                     RRAA"
     gene            4745..5491
                     /locus_tag="ACFM8U_001478"
     CDS             4745..5491
                     /locus_tag="ACFM8U_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC6334703.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETLQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLTQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5513..6100
                     /locus_tag="ACFM8U_001479"
     CDS             5513..6100
                     /locus_tag="ACFM8U_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC6334704.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6106..6285
                     /locus_tag="ACFM8U_001480"
     CDS             6106..6285
                     /locus_tag="ACFM8U_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC6334705.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            6517..6957
                     /locus_tag="ACFM8U_001481"
     CDS             6517..6957
                     /locus_tag="ACFM8U_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC6334706.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQSYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1906 a         1955 c         1668 g         1851 t
ORIGIN      
        1 gttaatccac tataaaaaca caacctaaat aaaaatgccg tctgaaccgt atttcaggtt
       61 tcagacggca tttgcgtgtc ggatgcacac cggacgggcg gtaagccggg ttctgtctcg
      121 gacagtcatt cctctaggca taccgttgcc ggtatgctca agcaacctac ccgaacgctc
      181 ggcgggcagc gtcattgcgt tctgtttggt cttgctccga atggggtttg gcctgccgca
      241 tattgttacc aaatgcgcgg tgcgccctta ccgcaccttt tcacccttgc ctgtactgcc
      301 aaagcagcca tcggcggttt tgctttctgt tccactttcc gtcgcgttac cgcgcccggc
      361 cgttaaccgg cattctaccc tgcggagccc ggactttcct ccccgtatgc cttacgcgat
      421 acgcggcgac tgtctgcccg tcccgtgtgc ggcgcggatt ataacacgaa acgcaaaaat
      481 gccgtctgaa acacaaaatg cttcagacgg catacggttc aaactacacg ccctgtttca
      541 ggctggcttc gataaagccg tccaaatcgc cgtccaatac ggctttggtg ttgcctactt
      601 cgtagcctgt gcgcaagtct ttgatgcgcg aggaatccaa aacatacgaa cggatttggc
      661 tgccccaacc tacatcggat ttaccttctt ccaacgcctg tttctcttcg ttgcgtttgc
      721 gcatttccaa ttcatacagt ttggatttca acatttccat cgcggcagct ttgttggcgt
      781 gttgcgaacg gtcgttttga cactgcacca caatccccgt cggctcgtgg gtaatacgca
      841 cggcggagtc ggttttattg atgtgctgac cacccgcacc cgatgcgcgg taggtatcga
      901 tacgcaaatc ggcggggttg atttcgattt cgatggaatc gtcgatttca gggtaaacga
      961 acacggaggc aaacgaggta tggcgtttgt tgttcgagtc aaacggcgaa tagcgcacca
     1021 agcggtgaac gccggtttcg gtacgcagca aaccataagc gtattcgcct tccacgcgga
     1081 tagtggcgcg gttgatgcct gcgatttcgc cgtcgtcttc ttcgaggatt tcgattttga
     1141 agcctttgcg ctcggcgtag cggctgtaca tacggaacag catacccgcc cagtcttccg
     1201 cttcggtacc gcccgcgccg gcggtgatgt cgataaagca gttgttcggg tcggcgggct
     1261 ggttgaacat ccgtttgaac tccaaatccg ccatctgttt ttccagcccc gccacatctt
     1321 cctgcacggc ggtaaaacct tcttcgtcgt tttcttcgac ggtcatttca atcagcatgc
     1381 ggttgtcttc gatgcccgaa gcgatgttgt cgagcgtcaa cacgatgcct tcgaggattt
     1441 tgcgctcttt gccgatttct tgggcgcgtt tcgggtcgtt ccaaagttcg gggtcttcgg
     1501 aaagaccgat aacttcttcc aatcggtctt tcttgccctg ataatccata taaacgcgga
     1561 tgtcttcgct gcgcttttcc aaatcgttca gggtattgtt gagctggttg attacttcgg
     1621 cttccatgat tcttttgttc tttcaaaatt ttaggggcgt attgtacggg attcgggtat
     1681 ttttttctat ggataaagcc ttctggaaac acgttcggac ggcatagcgt caataacggt
     1741 atgccgccag tttgcgtttg atttcaggca atgcggcacg tgctgcctcc tcgcccaacc
     1801 ggatggcgcg ttttttctga tcgaatccgc cgactgcacc caaatccaaa acctgtggtt
     1861 tgataactac atccgcctgc cccaactcat tttgcaacgc agaaacgctc attacgttca
     1921 gcgtctgatc gagataagag aagaaacctt ggctgatgtt tttgcccgga cgggcggaaa
     1981 tatcgacggc aatcacgaaa ttcgccccct gccgccgggc ggcactgacg ggcacgggct
     2041 gcgacagacc gccgtcaaca tatgtatgcc tgccgatgat aacgggttgg aacacattgg
     2101 gaatggcggc ggaagcgcgc acagcctgcc cggcattccc ctgattgaaa gcgacggcct
     2161 tgccggtttc aaaatcagta gcaacggcgg caaatttgat gggaaactgc tgaatctgcc
     2221 tgccgccgac ttttcggttg atgtaatttt gcagcttttc gcctttgata aaaccactgg
     2281 tggacaaggt taaatcgacc aaatcggttt tgcctaaaat ttcggcttcc aattcgaggc
     2341 ggtcgggcga catacccgat gcaaaaaggc tgccgacaat cgaacctgcc gatgtaccgg
     2401 taaccacctt cacaggaata ccgttttctt tcaaaacctt aataatacct acatgggcaa
     2461 atcctttaga tgcgccgcca ccgagtgcca aaccgaccac tgcggcgggt ttggcggttt
     2521 gcactagctt gcgggcagca ttatttcccg ccgtgccgca ggcggcaagc aacgcggcgg
     2581 cggcgattgc caaaagcggt ctgatttttg aaaacgttac catattttcc attcctttat
     2641 atatcacacc ccgtcaaaaa gagggattgc ttttcttaac aacccccttt gacagccaag
     2701 caaatgggga ctttgttaag tcatcatcaa aattaatatt tctttttttc ctttacggaa
     2761 attatatttg aaggcatact atccaaggtg ggaattatct cacaacaccg ccgttatcca
     2821 aatatcccgc ctttttccct ttctttccat caaaatactt tctttttata ttcattaact
     2881 tgttaaatca ttggctgccg ggtgtcagtt tttccgacaa aatccgtcta atggggtatc
     2941 aacagaacca aaacaggaac acttaatgaa aatcggaaca acttggcaga cggcatccgc
     3001 tatgctggtt ttgcgtctgt ttgccgcata tgaatttttg gaatcgggtt tgcaaaaatg
     3061 gaacggggag aattggtttt ccgaaatcaa cgatcagttt ccattcccgt tcaacttgct
     3121 gccggacgcg ttaaactgga atctcgccat gtatgcggag cttttactgc ccgtactgtt
     3181 gcttttgggt ttggcaacgc gtctgtcggc attagggctg atggtcgtta ccgccgtcgc
     3241 ttgggctgcg gttcacgccg gttcgggtta caatgtctgc gacaacggtt ataaaatggc
     3301 tttaatttat atcgtggtat taatcccgct gcttttccag ggtgcgggcg gatggtcgct
     3361 ggatacgctg ctgaaaaaac ggttttgccc ccaatgccgt ctgaagcaag attgattcag
     3421 tcgtggaatc tgactttaaa cattccaacc ttatctcgtt aacttgatat tctgaaaagg
     3481 aaatagaaat gaacaaaaac attgctgccg cactcgccgg tgctttatcc ctgtctttgg
     3541 ccgccggtgc agttgctgcc aacaaaccgg caagcaacgc aacaggcgtt cataaatccg
     3601 cccatggctc ttgcggcgcg tccaaatctg ccgaaggttc gtgcggcgcg gctggttcta
     3661 aagcaggcga aggcaaatgc ggcgagggca aatgcggtgc gaccgtaaaa aaaacccaca
     3721 aacacaccaa agcatctaaa gccaaggcca aatctgccga aggcaaatgc ggcgaaggca
     3781 aatgcggttc taaataatcc caccccttca aaccaagccg cgtttttcag taaaatgcgg
     3841 cttttttaac ggcaaacaaa gattttttaa caagcacatc attcttttgt gccatccgaa
     3901 ccgggtaaaa atatgattca acacgcaggc ttgggctacc gccgcgactt ggcggaagac
     3961 tttctctcgc tttccgaaaa cagcccgata tgctttatcg aagccgcacc ggaaaactgg
     4021 ctgaaaatgg gcggctgggc gcgcaaacag tttgaccgtg tggcggaacg gctgccgctg
     4081 gcgttgcacg gattgtctat gtcgctgggc gggcaagcac cgctggatac tgatttgata
     4141 gacggcatca aagaaatgat gcgccgttac gattgcacgt ttttctccga ccatttgagc
     4201 tactgccacg acggcggtca tctttacgat ttgttgccgc tgccctttac cgaggaaatg
     4261 gtgcatcata cggcgcggcg tatccgcgaa gtgcaagacc gtttgggctg ccgcatcgcc
     4321 gtggaaaaca cgtcctacta tctgcattcc ccgcttgccg agatgaacga ggttgagttc
     4381 ctcaacgcgg ttgcacgtga ggccgattgc ggcattcatc tggacgtgaa caatatctac
     4441 gtcaacgccg tcaatcacgg cctgttgtcg ccggaggctt ttttgaaaaa cgttgacgca
     4501 gatcgcgtgt gctatatcca tattgccgga catgacgtgg aaacgccgga attgttgatt
     4561 gatacacatg gcgcggcagt tttgccgact gtttgggact tgctcgaact tgcctatgcc
     4621 aagctgccga cgattccgcc caccctgttg gaacgcgatt ttaatttccc gccttttgcc
     4681 gaactcgaag ccgaagtcgc caaaatcgcc gattatcaaa cgcgtgccgg aaaggaatgc
     4741 cgccgtgcag cctgaaacct ccgcccaata ccagcaccgt ttcgcccaag ccatacgcgg
     4801 gggcgaagcc gcagacggtc tgccgcaaga ccgactgaac gtctatatcc gcctgatacg
     4861 caacaatatc cacagcttta tcgaccgctg ctataccgaa acgctgcaat actttgacag
     4921 cgaagaatgg agccgtctga aagaaggttt cgtccgcgac gcgcacgccc aaacgcccta
     4981 ttttcaagaa atccccggcg agttcctaca atattgccaa agcctgccgc tttcagacgg
     5041 cattttggca ctgatggatt ttgaatatac ccaattgctg gcagaagttg ctcaaattcc
     5101 ggatattccc gacattcatt attcaaatga cagcaaatac acaccctccc ctgcggcctt
     5161 tatccggcaa tatcgatatg atgttaccga tgatttgcat gaagcggaaa cagccttgtt
     5221 aatatggcga aacgccgaag atgatgtgat gtaccaaaca ttggacggct tcgatatgat
     5281 gctgctggag ataatgggtt cctccgcgct ttcgtttgac accctcaccc aaacccttgt
     5341 cgaatttatg cctgaggacg ataattggaa aaatattttg cttgggaaat ggtcaggctg
     5401 gactgaacaa aggattatca tcccctcctt gtccgccata tccgaaaata tggaaggcaa
     5461 ttccccgggc caaaaccatc tatccgcata aaattacctt gttcccgata ctatgccgct
     5521 acccgacctg accgatgccg aattaataga gtcgcgtaaa ctgcttctgc attttgcgcg
     5581 gcttcagttg cccgaccacc ctgatttggc tgaagattta gtgcaggaaa cattgctgtc
     5641 cgcatacagc gcaggcgaca gttttcaagg cagggcactt gtcaacagct ggctttttgc
     5701 catattgaaa aacaaaatta ttgacgcatt acgtcaaatc ggaaggcaga ggaaagtctt
     5761 taccacactg gatgacgagc tactggatga agcatttgaa agccattttt cccaaaacgg
     5821 gcattggacg ccggaagggc agccgcaaca ttggaacact ccggaaaaat cattaaacaa
     5881 caacgaattc caaaaaattc tgcaaagctg cctatacaac ttgcctgaaa acaccgcacg
     5941 ggtatttacc ctgaaggaaa tactcggttt ttcatccgac gaaatacaac aaatgtgcgg
     6001 tatcagcacg tccaactacc acaccattat gcaccgcgcc cgagaatcat tgcgccaatg
     6061 cctgcaaatc aaatggttca accaagaaaa cccgaagtaa acgttatgaa aaaatgccgc
     6121 gatatcgccc tgcttctttc caaacatcag gaccgggaaa ccaccccggg cgagaagatt
     6181 tccatataca cacacctgct gttctgccca cattgccgag aatataaaag acagcttcaa
     6241 accatcaaaa tatcactggc aaaaacaacc agaacttcaa aataaatgcc gtctgaaaag
     6301 gcttcagacg gcataagctg acggaaacaa atcaaaccga tttactgtta tcggcagttc
     6361 atccataata cacgcttcaa aagcagcata ttcctccata cggaatatat aaatacgcaa
     6421 aacacgaagg ctgcatcaat ttgctttatt tgccttattt cacagacggc gctacccctc
     6481 ccgcccaacc cgttctttct gaatgagcag atttcaatga ttaaggaaac cctaatgcgc
     6541 ccaatcttcc tatctttcgt tttactccct attttgataa ccgcctgcag cacaccggac
     6601 aagtctgccc gatgggaaaa tatcggcaca atctcaaacg gcaatattca tacatatatc
     6661 aataaagaca gcgtgagaaa aaacggaaat ctgatgattt tccaagataa aaaagttgtt
     6721 accaatctaa aacaagaacg ttttgccaac acccccgcat acaagactgc cattgccgag
     6781 tgggaaatcc actgcaacaa caaaacatac cgcttaagtt cgctacagtt gtttgataca
     6841 aaaaacacgg aaatttccac acaaagctac acagcctctt ccctccgccc gatgagcatc
     6901 ctgtccggga cattaaccga aaaacaatat gaaaccgtat gcggaaaaaa actctgattg
     6961 caacttatat acaaacttac ccacaaacct tatcataaaa atgccgtctg aaatactgaa
     7021 atatcagcat ttcagacggc attttgccat tccctgaaaa ttatccacaa agttatccac
     7081 attatttttt aaaaccggct tccatccgaa atatagtgga ttaacaaaaa tcaggacaag
     7141 gcgacgaagc cgcagacagt acagatagta cggcaaggcg aggcaacgcc gtactggttt
     7201 aaagttaatc cactatataa actcgctata caatttcact atccaaacgt aaattgttcc
     7261 attgatacac aaaacttctt acccccataa ttttgataaa gcatttctta cattcccggc
     7321 tccgtcccgt aaccaacaca gcctctaaaa aataatcgct tgttttgaca ccgatacact
//
LOCUS       DAXHSJ010000104         7352 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000104 DAXHSJ010000000
VERSION     DAXHSJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.104"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(108..2711)
                     /gene="pepN"
                     /locus_tag="ACFM8U_001482"
     CDS             complement(108..2711)
                     /gene="pepN"
                     /locus_tag="ACFM8U_001482"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC6334707.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYMLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPARYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNIFELTVKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQTFQFEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAHEALLDTLAAHFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLCADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2923..3717)
                     /locus_tag="ACFM8U_001483"
     CDS             complement(2923..3717)
                     /locus_tag="ACFM8U_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC6334708.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGKLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWSEFVQAWK"
     gene            complement(4467..5336)
                     /locus_tag="ACFM8U_001484"
     CDS             complement(4467..5336)
                     /locus_tag="ACFM8U_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC6334709.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGSLAYFLVKPRRRIGEIN
                     LAKCFSEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKMLDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5387..5923)
                     /gene="ruvC"
                     /locus_tag="ACFM8U_001485"
     CDS             complement(5387..5923)
                     /gene="ruvC"
                     /locus_tag="ACFM8U_001485"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC6334710.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAS
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5926..6165)
                     /locus_tag="ACFM8U_001486"
     CDS             complement(5926..6165)
                     /locus_tag="ACFM8U_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC6334711.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6196..7206)
                     /gene="dusB"
                     /locus_tag="ACFM8U_001487"
     CDS             complement(6196..7206)
                     /gene="dusB"
                     /locus_tag="ACFM8U_001487"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC6334712.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHKNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAGKTDRWACAYRQN
                     AV"
BASE COUNT         1655 a         1938 c         1926 g         1833 t
ORIGIN      
        1 atatgacttt gcggccgttt tgccattgcg taataaaacg atggggaagt gatgataaaa
       61 cgtgtgtgta actatatcag acggcattgt ttttctgttt gacggcctca atccaaaatt
      121 ttgccgacga tttcgcccac gtctttcgac aatccttcct gcgcccgaat gcgctgcaat
      181 gcttgtttca ccaagttttt gcggtgcggc tcgagcttgt tgcagaggtt gaacgcctgc
      241 accaaacggg cggcgacctg cgggttgaag cggtcgattt cgatgacttt gtcggcgatg
      301 aagcggtagc cgctgccgtc ttctgcgtga aaatgcggga cgttgcggct gaagctgccg
      361 atgagcgaac gggctttgtt ggggttttcg aggctgaatt tcggatgctg caaggcggtt
      421 tgaacctgtt gcagggtgtc gctgcggcgg cttgagccga taagggcgaa atatttgtcc
      481 atcaccagcg cgtcgtctga aaacttgtcg gcaaactgcg ccagcaggcg gttgcgcgta
      541 tcgctttcgt tgccgttaac ggcggacagg atgccccatt cgtgggtcat gttttgcgcc
      601 atttcgccgt atttttcggc aacggtttcg atgtgcgcgg ggtcggcgca caggacgaag
      661 gcgcggcaga cgttgcgcag cgtgcgccag ccggcagctt cggggctgta ctcgtagctt
      721 tggttttcct gcttcgccgc ctgacggttc aattcgtgcc atttcggcag gaagtgggcg
      781 gcaagcgtgt ccaacaaggc ttcgtgcgcc tgatggtagc gcaacgggtc gatgttttcc
      841 gtgccgtccc acagctcggc ttcagacggc acgcccaaaa gcagggcttt gaaggcgttg
      901 tccaagaggt cgtctgaaat gactttttcg acggcggcaa gcagtttttc gtgtttcggc
      961 aacccgatgc cgtctgaaag cgcggcaagg ttggcggcga cggcgcggcg gtagagcgtt
     1021 tgggcggctt cccagcgcgt gaaggcgtcg ctgtcgtggg cgagcaggag cagcaggtcg
     1081 tcgtcgctgt acggatagtt caggtgcacc ggcgcgctga agccgcgcag cagcgaggga
     1141 acgacggctt cggtcacgcc ttcgaactgg aaggtttgtt cggcttcggt cagcagcaat
     1201 acggcttcgg tcgcgcgttt gccctgatag tcgaatgccg ccgcttcgcc gttgcggttc
     1261 agcagcccga ttttgacggg aatcatcatc ggctgcttgt ccgccatatc gggcgtaggc
     1321 ggcacggttt gtttgacggt taactcgaaa atattgtttt tcagacggcc ttcggcttcc
     1381 aaaacgggcg tgcccgcctg gctgtaccac aaggcgaact ggtcgagatt gatgccgttc
     1441 gcgtccgcca tcgccgcgcg gaaatcgtcg caggtcacgg cctgcccgtc gtggcgttgg
     1501 aaatagagct tcataccttt ttggaagccc tcttcgccga gcaaggtgtg atacatccgc
     1561 accacttccg cgcctttttc ataaacggtc atggtgtaga aattgttcat ctcctcatat
     1621 cgggcggggc gcaccggatg tgcggtcgga cctgcgtctt cggggaactg gtgctggcgc
     1681 aaaaggcgga tgttttcgat gcggcgcacg gcgcggctgg cgcggtcgcc ggaaaattct
     1741 tggtcgcgga acacggtcag cccttccttc agcgaaagct ggaaccagtc tcggcaggtt
     1801 acgcggttgc ccgtccagtt gtggaaatat tcgtgtccga ccacggattc gatgccttca
     1861 aaatcggtat cggtggcggt gcggctgtcg gcaaggacga acttggtgtt aaagatgttc
     1921 aaacccttgt tttccatcgc acccatattg aaatcgccca cggcgacgac catgaaaata
     1981 tccaagtcgt attccaagcc gaagcgcgtt tcgtcccact tcatcgcgtt tttcaaggat
     2041 tccacggcaa agccgacctt gggcttgtcc gcttcggtgg tgtaaaactc gattttgacg
     2101 tttctgccgc tcatggtggt gaaatagtct tccgttaccg ccaaatcgcc cgcaaccaaa
     2161 gcaaacagat agctcggttt ggcaaacggg tcttcccatt tcacccaatg gcggccgtct
     2221 gaaaactcgc cgccgtcgat tttgttgccg ttagagagca aaacgggata gcgttttttg
     2281 tccgcgacga tggtggtcgt gaacttggac atgacatccg gacggtcgat atagaacgtg
     2341 attttgcgga agccctccgg ctcgcactgg gtaaacaggt taccggcgga cgcatacagc
     2401 cccatcagcg atttgttttc cgccggcagg atttcggttt ccacttcgac ggtgaagcgt
     2461 tcggacggca cgtctgcaat cgtcagcgtc tcgccttcca acatataatc cgccgccgcg
     2521 ccgttgattt tgacggacaa gagtttcgcc gaaccgtcca acaccagcgg ctcccctacc
     2581 ctctgcggct caaccgtcaa acgcgacttc acgacggttt gcggttcggc aatatcgaaa
     2641 tgcaggtcgg tttcgagaat gcggtaggca ggcgtttggt aatctttcag ataacggacg
     2701 gttttgctca tttttttctt tcaatattgt ttggtttgac tggaaaaggc ttcagacagc
     2761 acggatacag cttttaaagc ggattaacaa aaatcaggac aaggcgacga agccacagac
     2821 agtacagata gtacgggctg taccggtttg aatttaatcc accatatgcc gtctgaaacc
     2881 gaaacggcgg cacaagcgta ccgccttcgg acagcaggtt ttctatttcc acgcttggac
     2941 aaattctgac caatgcggtt tatcttctgt ttgggcggaa agatagtttc tgatccgctt
     3001 gatttccatt tcgtattcgt ccgcgtcgaa tctgccgctg accagacaga aacgcagata
     3061 catcaaatac gtattcacgg catcggtttc gcaataattg cggatttcct tcaatttacc
     3121 ggcatggaat gcctcccaga ccttgctgcc gtccataccc aatttacccg gaaaaccgca
     3181 cagtttcgcc atatcgtcca gcggcacatt cgccctcggc tggtaaagcg cgagcaaatc
     3241 catcaaatcg cagtggcgtt ggtggtaacg gctgatgtag ttgttccact tgaaatcgcg
     3301 gctgtcgccg aaatctccgt cgcccatatc ccaatagcgc gcggcgttga tgccatatat
     3361 cagggagcgg taatgcagaa ccggcagatc gaaaccgccg ccgttccaac tgaccagttg
     3421 cggcgtatgt ttttcaatca actcgaaaaa tttagcaatg accacttcct cgccgtcatc
     3481 catctcgccg atggtgccga catgtacttt atcctgcccc caacgcatac agcacgaaat
     3541 cgccacaacc tgatgaagat gatgctgcat aaaatctccg cccgtctgag cacggcgttt
     3601 ttgctgggca aacagcacca cttcgtcgtc gggcagcgag gacggcagct cgtacaatgt
     3661 tcggataccc tggacatcag gtacggtttc aatatcgaaa gccaaaatcg tattcatggc
     3721 agtaccttgc attcaaaaac agacttgcgc ctattgtgtc attaaaaggc ggataaaaaa
     3781 agaggcaacc ccccacagga ttgccccaat acctcaaatc agagatttac gcttcacaaa
     3841 caatacaggc tttcgcctgc ggctttaccc gcgtagctca actctacgcc ggcaaacttt
     3901 cgtttcaccg tttccaatga aaccccgacc aatcgcaaga ctgaccggaa aatcctttca
     3961 tacggcattt cctgcctgtc gtgtaattcc atgtagcgaa atgtacgcca ttttctacgc
     4021 ttcgccaagc attttttaca atataaatgt caaaacatta attttataaa attgctgaaa
     4081 atattaaata tatggatttt tattttttat atttcaataa atataaattt aattttgatt
     4141 tatatttaaa tttaagcata aaatttcaaa tattaaaggt aatatgaaag gcgtatatta
     4201 aatatttatt tataacgcta tgtttttaaa gaaaattaat tttaatatat taactagatt
     4261 gtctgcatat attcatgggt ttgcggtatt tttttcacaa cctgcttcga attttccgac
     4321 caagtcttaa aaatattgtt tttgaaatac ttaaatagca gcgattataa aatgaaatct
     4381 gttcatataa tctgccattt cgcatttaaa aaaacaatca ggagtttcga ctcgaaacgc
     4441 ctgatatgtt ttgtaatttt acgtagtcag taaaaatcgg ggctgccttc cggtcgggtt
     4501 ttgaaacgct tgtgcagcca aaaatactgc tcgggatgtt cgcgcacgcg ttcctcgata
     4561 aaacggttca tgcgctgcgc gtcggcttgc gcatcttcac tcggaaagga tttccaagca
     4621 gggtagaaat gcaatgtaac cgtattgtct gcctcgcgga cgggaatggc gggtatcact
     4681 tttgcatttg caagcgcggc aatgcggctc aatccggtaa tcgttgccgt ctgaataccg
     4741 aagaaatcca caaaaaccga atcgttgcgt ccgaaatcct gatcgggcag atacagaaac
     4801 ggcgcgctgc ttttgcggaa ctgtttgacg agggcgcgca gcccttcggt gcgcccgata
     4861 aggaagacgt tgtgatagcg gttgcggcct ttcaaaatct gttcgtccaa catcttgttt
     4921 ttttgatgag aatacatact gatcagcggg acatcctgat taagcgcgta caccgccatc
     4981 tcgaacgcgg tgaagtgcgg atacagaatg atgacttttt cccccgccgc cagcgcgtcg
     5041 tccaaataat gcttattgcg gtagcgcacc agcgatttca aacgtccggc aggcgcgtac
     5101 caatataaac cgtattccaa catcagtttc gccatgtgtt tgaaatgctg tttcaacacg
     5161 gttttacgct tttcctcact ccattcggaa aaacattttg ccaaattgat ttcgccgata
     5221 cggcggcgcg gtttgaccag aaagtaggca agcgaacccg tcaggtcggc aatcttgtgc
     5281 agcagcgcaa acggcagaaa ctgcaaaaca tacagtacaa aaaatataaa tttcatctcg
     5341 atacacattt tcttttcaga cggcaaaata caaatgccgt ctgaaactat tgaaacctgc
     5401 cgcgcttgac ctgcatcccc gaaggattga gtttggcggc aagcccgtgg ttgcgtaagg
     5461 cgtgggtcag cgcgacggca agaccgtccg ccgcatccgc ctgcggcgtt cccgaaagcc
     5521 ccagcatctg caccaccata tgctgcacct gttctttcgc cgccttgcct ttgccgacta
     5581 ccgcctgttt gacctgcaag gccgtgtatt ccgaaacggg cagcttatgg ctgaccaatg
     5641 ccgccaatgc cgcgccccta gcctgaccca gcatcagcgt cgatgcagga ttgacgttga
     5701 cgaacacctg ttccactgcc gcctgttgag gcttgtaaac ggcaacgact tcgccgatat
     5761 gccgcacaat cacggcaatc ctgtctgcca gagacgcatc ggcaggcgtt ttgatgcagc
     5821 cggaggcaac ataaaaatga tcccgccccc tgacatcgat gatgccgaaa cccgttacgc
     5881 ggctgcccgg gtcaatgccc aagatacgga cggttgccga catattcaca acaaaccgtg
     5941 ttgaatcagt tttttacgca aggtattgcg gttcagcccc agcatcacgg aggctttgga
     6001 ctggttgccg ccgcattgct ccatcacgca caccagcagc ggtttttcca cctgatgcaa
     6061 gaccatatcg tacacgccgc aaggttcggt accgttcagg tctttgaaat attgttccaa
     6121 attttgtctg atacattggg aaatatcggg aagggtatgg ggcataatcg cgctttcaaa
     6181 aggataatcg ggtgttcaga cggcattttg gcggtaggcg cacgcccaac ggtcggtttt
     6241 tccggcaagt ctttcaagat aacctgcaag catgtcgtat tgcgccgccg cactgtccaa
     6301 gcggttgatt tcacgacgtg tctgttcgcc gtcgggcatt tcgtcgatgt accagcctat
     6361 gtgtttgcgt gcaatgcgca caccggcggt ctcaccataa aacgcgtgca tggcgcggac
     6421 gtggttcaaa atggcggcac tgcattctgc caaactcaag gcaggcggta aaacaccgtg
     6481 ttcggcgtaa tgtttcaaat cgcggaagaa ccacggtctg ccttgcgcgc cgcgccctat
     6541 catgatgccg tctgcggcgg tttgtttaag gacggcttgg gctttttgcg gcgaagtaat
     6601 gtcgccgttg acccaaaccg ggatgttcag acggcatttg gtttcggcaa tcaggtcgta
     6661 agccgcttcg cctttgtaca tttgcgtgcg cgtgcgtccg tggacggcaa gggcggcaat
     6721 gccgcaatct tcggcgattt tggcgatgac gggcaggttt ttgtggtcgt cgtgccaacc
     6781 caaacgggtt ttgagggtaa cgggtacgcc cgccgccttg accaccgctt ccaaaatggc
     6841 ggcaaccagc ggctcgttct gcatcagcgc gctaccggct tggacattgc agactttttt
     6901 agcgggacag cccatgttga tgtcgataag ctgcgcccca aggctgacgt tgtaacgcgc
     6961 ggcatccgcc atctgctgcg gatcgcttcc ggcaatctgc acggcaacaa tgccgccttc
     7021 atcggcaaaa tcgctgcggt gcaaggtttt tctagtattt ctgagcgtcg ggtcgctggt
     7081 cagcatttcg cacaccgccc aacctgcgcc aaaatctcgg caaagtcggc ggaacggttt
     7141 gtcggtaatg cccgccatcg gcgcaagtgc gatggggttg tcgataaaat agccgccgat
     7201 gtgcataatg ggtccgcgtt tcaaaaaagt gcgacattgt acatttttta agcaggattt
     7261 ccaatctccg gacgcgcccg cgattggggc gggcaccgtt ttatggcata atccgcacac
     7321 agatttcctg ccccgccact cacaggcggg ca
//
LOCUS       DAXHSJ010000105         7325 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000105 DAXHSJ010000000
VERSION     DAXHSJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.105"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(190..2052)
                     /gene="hscA"
                     /locus_tag="ACFM8U_001488"
     CDS             complement(190..2052)
                     /gene="hscA"
                     /locus_tag="ACFM8U_001488"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC6334713.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTLQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVKQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVHAQIEVKPSYGL
                     DDDTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            2223..3095
                     /gene="hpnD"
                     /locus_tag="ACFM8U_001489"
     CDS             2223..3095
                     /gene="hpnD"
                     /locus_tag="ACFM8U_001489"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC6334714.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            3174..4487
                     /gene="hpnE"
                     /locus_tag="ACFM8U_001490"
     CDS             3174..4487
                     /gene="hpnE"
                     /locus_tag="ACFM8U_001490"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC6334715.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALADLQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            4571..5290
                     /locus_tag="ACFM8U_001491"
     CDS             4571..5290
                     /locus_tag="ACFM8U_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6334716.1"
                     /translation="MATLSNKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            5455..6126
                     /locus_tag="ACFM8U_001492"
     CDS             5455..6126
                     /locus_tag="ACFM8U_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC6334717.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            6136..6504
                     /locus_tag="ACFM8U_001493"
     CDS             6136..6504
                     /locus_tag="ACFM8U_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC6334718.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            6501..7148
                     /locus_tag="ACFM8U_001494"
     CDS             6501..7148
                     /locus_tag="ACFM8U_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC6334719.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
BASE COUNT         1621 a         2125 c         2031 g         1548 t
ORIGIN      
        1 gaatcgttct ctttgagcta aggcgagcca acgctgtacc ggtttaaatt taattcacta
       61 tacacgccct tgatgacggt tttcatcgaa tagacaatgc ctgacttttc catatcgatg
      121 tttttcaagt gttttcgagc cttcagacgg catcaggtta tatctatgcc gtctgaaacc
      181 gtttaagtat caaatattat cgacactctg gcctgtcaac gcgcgttgga tgttgcggtt
      241 catgcgtttg gcggcgaaat tgtcggtaat actgccgagt ttggcggcgg cggcacggat
      301 gtcttcggct tttccgtctt tcagacggcc ttgcaaatcg gcgatgcctt gccgaatctg
      361 ttgcaattct tcggcatcca gcaaatcgct gtccaactcg agggcggcgt tgacggcatc
      421 ggtcaggctt tcggcttcga ccacggcttc ggcacgggcg cgtgccgcca tatcttcggc
      481 ggcattgctc atgctgtctt tgagcatttg ggtgatggtg tcgtcgtcca agccgtagga
      541 aggtttgact tcgatttgcg cgtgtacgcc ggtgctttgc tcttgggcgg aaacggacag
      601 cagcccgtcc gcatcgattt ggaaggttac gcggatacgc gccgcacccg ccgccatagg
      661 cggaatgccg cgcagagtga atttggcaag gctgcggcag tcggcaacca gttcgcgttc
      721 gccttgtacg acgtgtatcg tcatcgcggt ctgaccgtct ttgaaggtgg taaagtcctg
      781 cgcgcgcgcg gtggggatgg tggaattgcg cgggatgatt ttttccgcca agccgccgta
      841 ggtttccaaa ccgagcgaca aaggtgtaac gtccagcagc agccattcgc cgtcggtttt
      901 gttgcctgcg aggacgtttg cctgtatggc ggcgccgagc gcgacgactt cgtcgggatt
      961 gaggttgttc agcggggttt gtccgaagaa ggtggcgact gcctgttgaa cgtgcagcat
     1021 acgggtcgaa ccgccgacca taatcacgcc tttgacttcg tttttaccga caccggcatc
     1081 tttcaacgcc tgtttgaccg gctcgagcgt tttcatcacc aaatgctgcg tcaggttgtg
     1141 gaactcggcg cgactgatgc ttgtgtcgat tgccatgccg tctgaaagcg tcgcctgaat
     1201 gcgcgcttcg gtttgcgtgg ttaattgttc tttggcggcg cggacgagcg agagcaggag
     1261 ttggctgtct tgttcgttga gttgggagag tccgttttgt tcgagcaggc ggcagaacag
     1321 ccggtggtcg aaatcgtcgc cgcccaacgc gctgttgccg ccggtggctt tgacttcaaa
     1381 caggcctttg gtcagttgca atacggatac gtcgaatgta ccgccgccca agtcgtacac
     1441 gacaaacgta ccttccgagg cgttgtccag cccgtaggcg attgcggcgg cggtaggttc
     1501 gttgagcagg cgcaatacgt tcaaacccgc cagacgcgcg gcatctttag tggcctggcg
     1561 ttgcgcatcg tcgaaatagg cgggaacggt aatcaccacg ccgaccaaat cgccgcccaa
     1621 ggtttcttcg gcgcgcgatt taagggtttt gaggatttcc gccgacactt cgacaggcgt
     1681 tttcaccccc tgcagcgtat gcagttcgat aacgcgttga ttgtcaccga aacggtaagg
     1741 caggtaatgc gtattttgat gcagatcggc aagcgtccgc ccgataaggc gtttggcgga
     1801 gctgacggtg ttcagcgggt cggttttttg ggcggacagg gcggttttgc cgacttcaat
     1861 gccgccgttt tccagatagc ggacgacgga aggcagggta acgcgccctt cggcatcggg
     1921 cagacaggcg gcactgccgc tgcggacggt ggcgaccaag ctgttggtcg tacccaaatc
     1981 gatgcctgcc gccaaacggt gtcggtgcgg ggcggcggac atacccggtt ctgaaatctg
     2041 caaaagagcc ataaattctg cctgtcggtg cggtgtgtaa aggaaggaag tcggcatttt
     2101 aacagaattc gcgccgatag ttgagcaaac cgctaaggat tgggtcgtgc cgccgaaatt
     2161 gccctctacc gccaaatgcc gataaaatcc gcctttccct cccatcatta ggaatataaa
     2221 tcgtgaaagg actcgactat tgccgccaaa aagcagaaga aagccgctcc agttttttgt
     2281 cgggcttccg tttcctgact caggaaaaac gggatgcggt aacggtttta tatgcttttt
     2341 gccgcgaatt ggacgatgtg gttgacgaat gttccaaccc cgatgttgca caggcgacat
     2401 tgaactggtg gcgcggcgat ttggacaagg tattcggcgg cgcgatgccg gaacaccccg
     2461 tcaatcaggc cttgcggcag gttaaggaaa ccttcaagct gccgaaatat gaactggaag
     2521 ccttaatcga cgggatgcag atggatttgg ttcgggcgcg ttacggcagt tttgaagaat
     2581 tgaaactgta ctgccgccgt gttgcaggtg tagtcggctg cctgattgcg cggattttgg
     2641 ggttttcaga cggcaaaacg ctggaatacg ccgacaagat gggacttgcg ctgcaactga
     2701 ccaacatcat ccgcgatgtc ggcgaagacg cgcgcagggg acggatttac ctgccgatgg
     2761 aggaaatgca gcggtttgat gtccccgcaa gcgtgatttt gcaatgcagc ccgacgggca
     2821 attttgccga attgatggcg ttccaaatca aacgcgcccg tgaaacctac cgcgaagccg
     2881 tatcgctgct gccggctgcc gataaaaaag cccaaaaagt cggactggtt atggcggcgg
     2941 tttattacga gctattgaac gaaatcgacc gggacggcgc gcaaaacgtc ctcaaataca
     3001 aaatcgccct cccttcgccg cgcaaaaaac gcattgccct gaaaacctgg ttattcggat
     3061 tcaaaccgcg ccccggcacg ccggaacggg cataaggcgc ataccgcccg cccttcagga
     3121 aaacccaacc cccgccgccc cgaaaaacgg ggtttcaacc attggaggaa taaatgatga
     3181 acacgccgca tccgcgcccg aaaatcgccg tcatcggcgc aggctgggca ggactgtccg
     3241 ccgccgtcac cttggcgcgg cacgccgacg ttaccctgtt tgaagccggc cggcaggcgg
     3301 gcggcagggc gcgcgcactg gccggaaata ccgacggttt cggttttttg gacaacgggc
     3361 agcacatttt gctcggcgca taccggggcg tgttgcgcct gatgaaaacc atcggttcgg
     3421 atccccgtgc cgcctttttg cgcgtaccgc tgcactggca tatgcacggc ggtttgcagt
     3481 tccgcgccct ccccctgccc gcgccgctgc atattttggg cggcgtgctg cttgcccggc
     3541 gtgcaccgac tgcattcaaa gccaaactgc ttgccgatat gtccgatttg cagaagtccg
     3601 cacgcctcgg acagcccgac acgacagtgg cgcaatggct gaaacagcgg aacgtgccgc
     3661 gtgccgccgt gatgcagttt tggcagccct tggtttgggg cgcgctcaac acgcctttgg
     3721 aaaccgcaag cctgcgcgtg ttgtgcaacg ttttgtccga cggcgtgctg acgaaaaaat
     3781 ccggcagcga ctatctccta cccaagcagg atttgggcgc aatcgtcgcc gaacccgcct
     3841 tggcggatct tcaacggctc ggcgcggaca tccgcctcga aacgcgcgta tgccgtctga
     3901 acaccctccc ggacgggaaa gtgctcgtca acggcgaagc tttcgatgcc gccgtccccg
     3961 ccaccgcgcc ctaccacgcc gccgcgctcc tgcccgaagg cacgcccgaa cacgttcaga
     4021 cggcatatca aaaccttcgc taccacgcca tcaccaccgt ctatctgcgc tacgccgaac
     4081 ccgtccgcct gcccgccccg ctgaccggcc ttgccgacgg cacggtgcaa tggctgcttt
     4141 gccggggcag gctcggactg cctgaaaacg aagtgtccgc cgtcatcagc gtttccgacc
     4201 gcgtcggcgc gtttacaaac cgagcgtggg cggacaaagt tcacgccgac ctcaaacgca
     4261 tccttccgca tttgggcgaa cccgaagccg tgcgcgtcat caccgaaaaa cgcgccacaa
     4321 ccgcagccga tgccccgccg ccggatttgt cgtggctgca ccggcaccgc atcttccccg
     4381 ccggcgacta cctccacccg gactaccccg ccacgctcga agccgccgta caatcaggtt
     4441 tcgcggcggc ggaaacctgc ctgcaaagcc tgagcgatgc cgtctgaaaa cgccggctgg
     4501 tatcgggacg ctttacaagg tgcggcaaaa cgctaaaata cccccgaccg accaccaaaa
     4561 aggaaaaagt atggcgacac tgtccaacaa aaccatctta gttaccggcg catcgcaagg
     4621 tctgggcgaa caggtcgcca aagcctatgc ggcggcaggc gcaaccgtga ttctggtggc
     4681 acgccatcag aaaaaactgg aaaaagtcta tgacgcgatt gtcgaagccg gacaccccga
     4741 acccttcgcc atccgtttcg acctgatgag tgcggaagaa aaagaattca atcagttcgc
     4801 cgccaccatt gccgaagcca cgcaaggcaa actggacggc atcgtccact gcgccggcta
     4861 tttttacgcc ctctcgccgc tggatttcca aaccgtcgcc gaatgggtca accaataccg
     4921 catcaacacc gtcgcaccta tggggctgac ccgcgccctg ttcccgctgc tgaagcagtc
     4981 gcccgacgcg tccgtcatct tcgtcggcga aagccacggc gaaacaccca aagcctactg
     5041 gggcggcttc ggcgcgtcca aagccgcgtt gaactacctg tgcaaagtcg ccgccgacga
     5101 atgggaacgc ttcggcaacc tgcgcgccaa cgtcctcgtc cccggaccca tcaattcccc
     5161 gcaacgcatc aaatcccatc cgggcgaagc caaaagcgaa cgcaaaagct acggggacgt
     5221 gctgcccgca tttgtctggt gggcaagtac cgaaagcaaa gggcggagcg gcgaaatcgt
     5281 ttacctctaa atccggaaaa ggcaggcatc ccgcccgcgc cgcgattccg tttgaaaaat
     5341 atccgtactt tccgcgcgca tccgtcccgc acagacaaac accttgactt aaatttacat
     5401 aaggacaata atgataaccc gttatttcct taacttgacc ttagggtaaa ttttatgaaa
     5461 accgtttcca ccgccgttgt ccttgccgcc gctgccgttt cactgaccgg ctgtgcgacc
     5521 gaatcctcac gcagtctcga ggtagagaaa gtcgcctcct acaatacgca atatcacggt
     5581 gttcgtaccc cgatttccgt cggaacattc gacaaccgct ccagcttcca aaaaggcatt
     5641 ttctccgacg gggaagaccg tttgggcagc caggcaaaaa ccattctagt aacgcacctg
     5701 caacagacca accgcttcaa cgtactgaac cgcaccaatt tgaacgcatt aaaacaggaa
     5761 tccggcattt ccggcaaagc gcataacctg aaaggcgcag attatgtcgt taccggcgat
     5821 gtaaccgaat tcggacgcag agatgtcggc gatcatcagc tcttcggcat tttgggtcgc
     5881 ggcaaatcgc aaatcgccta tgcaaaagtg gctctgaata tcgtcaacgt caatacttcc
     5941 gaaatcgtct attccgcaca gggcgcgggc gaatacgcac tttccaaccg tgaaatcatc
     6001 ggtttcggcg gcacttccgg ctacgatgcg actttgaacg gcaaagtttt agacttggca
     6061 atccgcgaag ccgtcaacag cctggttcag gctgttgaca acggcgcatg gcaacccaac
     6121 cgttaaggag aaatgatgaa tccgaaaacc ttgagccgtt tgtcgctgtg tgcggcggtc
     6181 ttggctctga ccgcctgcgg cggcaacggg caaaaatccc tgtattatta cggcggctat
     6241 cccgataccg tctatgaagg tttgaaaaac gacgacactt cgttgggcaa gcagaccgaa
     6301 aagatggaaa aatactttgt ggaagccggc aacaaaaaaa tgaatgccgc cccgggtgcg
     6361 cacgcccatc tgggactgct gctttcccgt tcgggagaca aagagggcgc gttccgccag
     6421 tttgaagaag agaaaaggct gtttcccgaa tcgggcgtat ttatggactt cctgatgaaa
     6481 accggtaaag gaggcaagcg atgaaacctt tgattttagg gcttgccgcc gtgttggcgc
     6541 tgtctgcctg ccaagttcaa aaagcgcccg atttcgacta cacgtcattc aaggaaagca
     6601 aaccggcttc aattttggtg gttccgccgc tgaacgaatc gcccgatgtc aacggaacat
     6661 ggggtgtact ggcttcgacc gccgctccgc tttccgaagc cggctattac gtcttccccg
     6721 ccgcagtcgt ggaggaaacc ttcaaacaaa acggcttgac caatgccgcc gatattcacg
     6781 ccgtccggcc ggaaaaactg catcagattt tcggcaatga tgcggttttg tacattacgg
     6841 ttaccgaata cggcacttca tatcaaattt tagacagcgt gacgaccgta tccgccaaag
     6901 cacggctggt cgattcccgc aacggaaaag agttgtggtc gggttcggcc agcatccgcg
     6961 aaggcagcaa caacagcaac agcggcctgt tgggggcttt ggtcagcgca gtggtcaatc
     7021 agattgccaa cagcctgacc gaccgcggtt atcaggtttc caaaaccgcc gcatacaacc
     7081 tgctgtcgcc ctattctcac aacggcatct tgaaaggtcc gagattcgtt gaagagcagc
     7141 ccaaataagc atatgccgtc tgaacctctc cgagcttcag gcggcatatg cgaatcttga
     7201 tacttgcaag ccgcaccgcc caaaatcggg cagccggttt caatatccga acaaggttcg
     7261 ggttctgtat tttgcagaac ccgaaccttt tattcaaatt taaacttaat tccggcgtgg
     7321 ttgta
//
LOCUS       DAXHSJ010000106         7318 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000106 DAXHSJ010000000
VERSION     DAXHSJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.106"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            591..1118
                     /locus_tag="ACFM8U_001495"
                     /pseudo
     CDS             591..1118
                     /locus_tag="ACFM8U_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation factor family protein"
     gene            1192..1494
                     /locus_tag="ACFM8U_001496"
     CDS             1192..1494
                     /locus_tag="ACFM8U_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334720.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGTD"
     gene            1841..3472
                     /locus_tag="ACFM8U_001497"
     CDS             1841..3472
                     /locus_tag="ACFM8U_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6334721.1"
                     /translation="MFIILGRNFLKIILCFSFFVSKFALASVNAPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAKLSTRAVPYVGTALLAHDVYETFKEDIQARGYQYDTETDKFV
                     KGYEYSNCLWYEDERRINRTYGCYGVDSSIMRLMSDYSRFPEVKELMESQMYRLARPF
                     WNWHKEELNKLSSLDWNNFVLNSCTFDWNGGDCSVNKGDDFRNGANFSLIRNPKYKEE
                     MDAKKLEEILSLKVDANPDKYIKATGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVV
                     ATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPANNPNPNENPGTS
                     PNPEPDPDLNPDANPDTDGQPGTRPDSPAVPGRTNGRDGKDGKDGKDGGLLCKFFPDI
                     LACDRLPESNPAEDLNLPSETVNVEFQKSGIFQDSAQCPAPVTFTVTVLDSSRQFAFS
                     FENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
     gene            4417..4701
                     /locus_tag="ACFM8U_001498"
     CDS             4417..4701
                     /locus_tag="ACFM8U_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC6334722.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     NSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            4702..5889
                     /locus_tag="ACFM8U_001499"
     CDS             4702..5889
                     /locus_tag="ACFM8U_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC6334723.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMAGDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIVLLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEQQAVLPDKTEGEPVNNGNLTADMFVPTLTE
                     KPESKPIYNGVRQVKTFEYIAGCVQGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVRQSAQQHSDRAQVATLGGKPLQNLMYDNWQERGKPFEGIGGGVVG
                     SAN"
     gene            6104..7060
                     /locus_tag="ACFM8U_001500"
     CDS             6104..7060
                     /locus_tag="ACFM8U_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HFC6334724.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLHQHQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT         2208 a         1518 c         1767 g         1825 t
ORIGIN      
        1 aatgacggga tttgaggttg cagcatttat cgggagcaac agaatccgct ctgccgtcat
       61 tcccacgaaa gtgggaatct agttttttga gtttcagtca tttccgataa attgccttag
      121 cattgaatgt ctagattccc gccttatatg atgcgctcta tcaaaggggc gcattaattt
      181 tcttaacatt cccctttgac agccaagtga aaggggcttt tttatgtcag cagtaaatgt
      241 aatattttcc tgttcttatt ggagaatatt taaaaaatca gattcttgtg ttttgtgttt
      301 ttatcagttc agacatggcg aaccgcataa actcattaat caagagaatt tttcaaagct
      361 ttatcaggcg ttcgattata tagattcggt tggttcgaat tttccagtga ttatcacaac
      421 ggatggttgt ggtctttttt gttgatcttt aaaagtttgt caggatttgg ctttcggtcg
      481 ttgaccgtcg tacgcgcttt agcgcggaag actggaaacg gctgaaagcc ccccccttga
      541 ctaacagggg gggagcgaaa ttaaaaacca attccaagag tagtgaacga atgagtgaag
      601 ttgaatattt ctcacacttt atatcggacg gaaaagggaa gcttttagaa attccgcagc
      661 gaagaggtaa gcaagacggg gtttttgttg attggatttc attcacattc catgaagata
      721 ctttactgaa agtttccggt tgccctttat tttctgatgc tgaatacatg tatgtattaa
      781 gcagaaagct ggaagaaatt ctaggttttg gcataacgcg caaatgcaaa tcaaggggca
      841 acaaattcta tgaatccatg tataggttag gttcggatga tgttgattat ggagaggtgc
      901 atttcggagg tcagcgcaat actgttttag ttgagttgaa aggtactggt tgcagcgttg
      961 caagtccggg ttgggagttg aggctaaagc agtttctcga tgattcggta aggacaagaa
     1021 taacgcgaat tgacctagca cttgattttt ttgatggaga gtacacgccg gatcaggcgt
     1081 tgttagatca cgataatggt ttttttgata acagcaatca aaggcttcaa gatggcttgg
     1141 agcatgatca aatctgtcaa ataaacagag tgaagaaaaa ggggcgcata aatgggctat
     1201 cgtgtcggca taaattgttt tgatacaaga ttgcaggcag acgactattt attgtcgtcc
     1261 cttcctccta ctgttaccca ggacggaaaa atcatcaggc cggaaagggt gggcgataaa
     1321 tggattttga acggaaagcc ggttacgttg tcttatccgg aatgttccaa ttttgagcag
     1381 ataaagcaag gttcttatgt cggttcgacg gttctaattc tgtttgtagt catttacggt
     1441 ttcaggcttc tgattaattt tttaaaagac ataggcaagg ttgggactga ttgatgatta
     1501 tagatttctg gtttcttctc ggtttcttct tggctttgtc tgttgcttgg ctgttttggt
     1561 aacggttggt agaatcggct ttttagagtg ttttaaaagg tccgaattat gtttatttct
     1621 gaatatcatt tagttaaatt tcaaactgat tcacatattt atagagattt accacaagcg
     1681 ttaatttatt atagggaatt gattagaaaa ggggttttta aaacttcgtt ttcatttgat
     1741 atttttagga atttctttca tcgttatgat agagatttta tagaaattca attccctgat
     1801 tcttctacat tattaattaa attagatgaa gcaaaatgtt atgtttatta tcctagggcg
     1861 aaatttttta aagattatcc tatgctttag tttttttgta tctaaatttg cattggcatc
     1921 agtaaatgct ccgggtaaat ttgatagggt tgaagtttat gatgatggca gatatttagg
     1981 tattcgaggt tcagatgaca aaagaagaag aatttggaaa ggtgtatttg atagagaatc
     2041 gggaagatat ttaacttcag aagctcaaga tttaaaagtt aggcatgtat ctactggagc
     2101 atcaagtacg ggtaaagtta gttcggttgt atcttcatca gtttcccgcg ctggcgtatt
     2161 ggcgggggtc ggcaaacttg cccgcttagg cgcgaaatta agcacaaggg cggttcccta
     2221 tgtcggaaca gcccttttag cccatgacgt atacgaaact ttcaaagaag acatacaggc
     2281 acgaggctac caatacgaca ccgaaaccga caaatttgta aaaggctacg aatatagtaa
     2341 ttgcctttgg tacgaagacg aaagacgtat taatagaacc tatggctgct acggcgttga
     2401 cagttcgatt atgcgcctta tgtccgatta cagcagattc cccgaagtta aagaattgat
     2461 ggaaagccaa atgtataggc tggcacgtcc gttttggaat tggcataaag aagaactgaa
     2521 taaattaagt tctttggatt ggaataattt tgttttaaat agttgcacat ttgattggaa
     2581 cggcggagat tgttcggtca ataaaggtga tgatttcaga aatggggcta atttttccct
     2641 tatccgcaat ccgaaataca aagaagaaat ggatgccaaa aagctggaag agattttatc
     2701 gttgaaagtc gatgccaatc ccgacaaata cataaaggca accggttatc ccggttattc
     2761 cgaaaaagta gaagtcgcac ccggaacaaa agtgaatatg ggtcccgtca cggacaggaa
     2821 cgggaatccc gttcaggttg tcgcaacatt cggcagggat tcgcaaggca acaccacggt
     2881 ggatgttcaa gtaatcccgc gtcccgactt gacccccgga agcgcggaag caccgaacgc
     2941 acagccgctg cccgaagtat cgcccgccga aaaccccgca aacaacccga accccaatga
     3001 gaaccccggc acgagcccca atcccgaacc cgaccccgat ttgaatcccg atgcaaatcc
     3061 cgatacggac ggacagcccg gcacaagacc cgattccccc gccgttccgg gacgcacaaa
     3121 cggcagggac ggcaaagacg gaaaggacgg caaagatggc ggccttttgt gcaaattctt
     3181 ccccgacatt ctcgcttgcg acaggctgcc cgagtccaat ccggcagaag atttaaatct
     3241 gccgtctgaa accgtcaatg tagagtttca gaaatcagga atctttcaag attccgcaca
     3301 gtgtcccgca cctgtcactt tcacagtgac tgtgcttgat tcaagcaggc agttcgcgtt
     3361 cagctttgag aacgcatgta ccatagccga acggctaagg tacatgcttc tcgcccttgc
     3421 ttgggcggtt gccgcctttt tttgtatccg cacagtatct cgtgaagtct agcaggcgca
     3481 gcaccgccgg gcttcagtaa cttgtaccaa ggcaggggga ggacgtccag aaagatttgt
     3541 aaagacggct ttatcgtctt tataaatctt tttggatacc ccttgccgcc ccgccaaaag
     3601 aacacattct gccgcaaggg caggtggtaa ggcgcgcgcc ttttgcgccg ttccccctgc
     3661 ccccgcggcg tcgcaagtga gactgggggt gcgggggcta gtccccgcaa agcctttcag
     3721 cttcggaagc cacggccgaa aggcaggcgc agcactgccg gtctgagcgg aagccaggct
     3781 acaggcaggc gcagcaccgc cgagctaggc ggaagccagg ctacaggcag gcgaagcacc
     3841 gccggttggg cggaagccac ggccgaaagg cagggcgaag caccgccagg cttaggcgga
     3901 agccacggcc gaaaggcagg cgaagtaccg ccggtctggg cggaagccat ggtaaaaggc
     3961 aggcgaagca ccgccgggct tcagtaacct ttgttcaggc agggggagga tgtccgtaaa
     4021 gaatcgtaaa ggcggggttt tttcgccttt atgattcttt atggataccc cttgccgctc
     4081 ggacaaagaa atacactctg ccgcaagggc aggtggtaag gcgcgcgcct tttgcgccgt
     4141 ccccctgccc ccgcggcgtc gcaagtgaga ctagggggtg tgggggacta gtcccccgca
     4201 aagcgttcag cttcggaaac tttggccgaa aggcaggcga agcagcgcac tttgcgacga
     4261 atgtcgcaaa tagccgagaa gcgcgggggg attggcgata agcgcgaggg gggtgtcccc
     4321 acagcgccgc cgcgccgcga atgcggcgca aaatctttca gattaagaaa catttgttta
     4381 atgaggcaac cgtgcctttt aagaaaggga tagcaaatga aattgttggc cgcattgatt
     4441 ccgcttttga tgagcgtggc aggccgtata ttgactgcat taggcttgat ggcggtaacc
     4501 tattcagggg tggatagatt ggtagcccat tttcagcagg cgataaccaa tagcataacg
     4561 ggcgcgcctc aagcgatgtt gcagcttttt tatataagcg gcggtggaac cgttcttaat
     4621 atcctgtttg gtgcgatcgc ctttattctg tcatttaaac aaatgacaaa actagcaacc
     4681 tcaatcggga agaaaaaata aatggcagag atctgtttga taacaggcac gcctggttca
     4741 gggaaaacat taaaaatggt ttccatgatg gcaggcgatg aaatgtttaa gcctgatgaa
     4801 aacggcatac gccgtaaagt atttacgaac atcaaaggct tgaagatacc gcacacctac
     4861 atagaaacgg acgcgaaaaa gttgccgaaa tcgacagatg agcagctttc ggcgcatgat
     4921 atgtacgaat ggataaagaa gcccgaaaat atcgggtcta ttgtcattgt agatgaagct
     4981 caagacgtat ggccggcacg ctcggcaggt tcaaaaatcc ctgaaaatgt ccaatggctg
     5041 aatacgcaca gacatcaggg tattgatata tttgttttga ctcaaggccc taagcttcta
     5101 gatcaaaatc ttagaacgct tgtacggaaa cattaccaca tcgcttcaaa caagatgggt
     5161 atgcgtacgc ttttagaatg gaaaatatgc gcggacgatc ccgtaaaaat ggcatcaagc
     5221 gcattctcca gtatctatac actggataaa aaagtttatg acttgtacga atcagcggaa
     5281 gttcataccg taaataaggt caagcggtca aagtggtttt acactctgcc agtaatagta
     5341 ttgctgattc ccgtgtttgt cggcctgtcc tataaaatgt tgagcagtta cggaaaaaaa
     5401 caggaagaac ccgcagcaca agaatcggcg gcaacagaac agcaggcagt acttccggat
     5461 aaaacagaag gcgagccggt aaataacggc aaccttaccg cagatatgtt tgtcccgaca
     5521 ttgacggaaa aacccgaaag caagccgatt tataacggtg taaggcaggt aaaaaccttt
     5581 gaatatatag caggttgtgt acaaggcgga agaaccggat gcacctgcta ttcgcatcaa
     5641 gggacagcat tgaaagaagt gacggagttg atgtgcaagg actatgtaaa aaacggcttg
     5701 ccgtttaacc catacaaaga agaaagccaa gggcaggaag ttcggcaaag cgcacagcaa
     5761 cattcggaca gggcgcaagt tgccacgttg ggcggaaagc cgctgcaaaa tcttatgtat
     5821 gataattggc aggagcgagg aaaaccgttt gaaggaatcg gcgggggcgt ggtcggatcg
     5881 gcaaactgaa gaaaacggca agagagaaaa aagacccata aaccgtttga atatagacgg
     5941 cttacgggtc tttgtttcgc gcaaagcaaa ggctaaggca gtcaggcagc aaatcccgca
     6001 atgtatcaaa acagacgcgt agaaatgccg gctgccttta tccatcctca aaattgaata
     6061 tcatcctagc cgtatcaagg ctgtataaat aaggaaaata ccaatgaata taatcgggct
     6121 ggacatctca aaggacacca tagacgcaac attgcataaa acaaacggaa gtatccatta
     6181 cattaaattt aagaataatg atgatggatt aaaacagttt agattgtgga taaagggaaa
     6241 cagaatcaga aaagcctata tcggcatgga ggcaacaggc atctattacg aaaaggcagc
     6301 agatatgctt tcttcctact atactgttta cgttattaat cccttaaaaa tcaaggacta
     6361 cggaaaaagc aggtttaacc gtaccaaaac cgacaaagca gattcaaacc tgatagcaga
     6421 ctacataaaa aggcatcaag atacattgat accgtatcag atacccaaaa acaaagcact
     6481 gcaaaaactg attaacctta aaaatcaatt acatcaacat cagaagcaaa ttaaaaaccg
     6541 tcttcatagc actgaagaag acttcataag gaacatacat caagacttga tagataccat
     6601 acaggacaag atggaacagg taaaaatagc catatccgaa caaatcaaaa aacaaacgga
     6661 caataaccat taccgcaatc ttcaaaccat cccgagcata ggcaaagaca ccgcatcagt
     6721 tctttatgcg caactgacag aaaaacattt taaaaccgca aaccagtttg tatcctatgc
     6781 cggattaagt cccgccatca tacaatcagg gacaagcgta agaggtcggg gcagattgag
     6841 ccgatacgga aacagacgat taaaaagtac gctgtatatg cccgcccttt gtgcttaccg
     6901 ttttaacgca tttccgaaat taataaataa tctgaaaaaa gcgggtaagc caaagatggt
     6961 aatcatcgtt gccatcatgc gcaaactggc gaagctcgcc tattacattg ttaaaaccgg
     7021 ccagccttac gatgcggaaa gacaccgatt gaatcaataa aattcaacaa aattaaacgg
     7081 ttacgcgaat atatttgtgt aaccgtgcat ttgcatatcg taaataaacg taaataaaaa
     7141 taacaatata aatcagtata ttgcaacttt gttttttatt ttgtgttgac gggcaacata
     7201 tcatctgcgc gggaatgacg aatccatcca tacggaaacc tgcaccacgt cattcccacg
     7261 aaagtgggaa tctaggacgc aaaatctcaa gaaaccgttt tacccgataa gtttccgt
//
LOCUS       DAXHSJ010000107         7270 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000107 DAXHSJ010000000
VERSION     DAXHSJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.107"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..327
                     /locus_tag="ACFM8U_001501"
     CDS             <1..327
                     /locus_tag="ACFM8U_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6334725.1"
                     /translation="TEEGIAQTVVKGKTTRAEVEARFGKRNPFGCYAYHEVSLPIYNF
                     LPTNFIYMKSERRHWEWCVDYDGEGVVRDYRFTHKVEKDERSVIRDAVGVIRKEAGKS
                     LSQPEK"
     gene            391..1674
                     /gene="hemL"
                     /locus_tag="ACFM8U_001502"
     CDS             391..1674
                     /gene="hemL"
                     /locus_tag="ACFM8U_001502"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC6334726.1"
                     /translation="MNRNEILFDRAKATIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENSTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTARTEQLVQG
                     FQTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRNVAF
                     GPSAYEAGFVSAAHTPVLIDETVAVAVEVFKAMAA"
     gene            complement(1927..2490)
                     /gene="orn"
                     /locus_tag="ACFM8U_001503"
     CDS             complement(1927..2490)
                     /gene="orn"
                     /locus_tag="ACFM8U_001503"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC6334727.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVSGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2508..3395)
                     /gene="prmA"
                     /locus_tag="ACFM8U_001504"
     CDS             complement(2508..3395)
                     /gene="prmA"
                     /locus_tag="ACFM8U_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC6334728.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSATIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAIRASKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQIEELSGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            complement(3513..4874)
                     /gene="accC"
                     /locus_tag="ACFM8U_001505"
     CDS             complement(3513..4874)
                     /gene="accC"
                     /locus_tag="ACFM8U_001505"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC6334729.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTILLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKIGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEEERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(4987..5448)
                     /gene="accB"
                     /locus_tag="ACFM8U_001506"
     CDS             complement(4987..5448)
                     /gene="accB"
                     /locus_tag="ACFM8U_001506"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC6334730.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAAAPVY
                     AAPVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEV
                     GQQVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(5430..5687)
                     /locus_tag="ACFM8U_001507"
     CDS             complement(5430..5687)
                     /locus_tag="ACFM8U_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC6334731.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            5804..6844
                     /gene="queA"
                     /locus_tag="ACFM8U_001508"
     CDS             5804..6844
                     /gene="queA"
                     /locus_tag="ACFM8U_001508"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC6334732.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSHLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVTVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT         1487 a         1820 c         2186 g         1777 t
ORIGIN      
        1 acggaagagg gaatcgcaca aacggttgtt aaaggcaaaa cgacccgggc ggaggtagag
       61 gcacgtttcg ggaagcgcaa ccctttcggt tgttatgcct atcacgaggt cagcctgccg
      121 atttataatt ttttgccgac caatttcatt tatatgaaat cagaacgccg gcattgggag
      181 tggtgtgtgg attacgacgg ggagggagtc gtccgggact accgctttac acataaagtg
      241 gaaaaagacg agcgttccgt catccgggat gcggttggcg tgatccgcaa agaagcgggc
      301 aaatccttgt cgcaacctga aaaatgataa aatagctttt tctgtttcag aacctaaaac
      361 ctgcctttag gatggaattt aaggataaat atgaaccgta acgaaatttt attcgaccgc
      421 gccaaagcca ccatccccgg cggcgtgaat tcccctgtcc gcgcattcgg cagcgtcggc
      481 ggcgtgccgc gcttcatcaa aaaagccgaa ggcgcgtatg tttgggacga aaacagcacg
      541 cgttataccg actatgtcgg ctcttggggg cctgcgattg tcggacacgc gcaccccgaa
      601 gtcgtcgaag ccgtgcgcga agccgcgttg ggcggtttgt cgttcggcgc gcccaccgag
      661 ggcgaaatcg ccattgccga acaaatcgcc aaaatcatgc cgtccgtcga acggctgcgc
      721 ctcgtcagct ccggtaccga agcgacgatg accgccatcc gtttggcacg cggttttacc
      781 ggtcgcgaca aaatcatcaa gtttgaaggc tgctaccacg ggcattccga cagcctgctg
      841 gtgaaagccg gcagcggcct gctgacgttc ggcaatcctt cttccgccgg tgtgcctgcc
      901 gactttacca aacacacttt agtccttgag tacaacaaca tcgcccaatt ggaagaagcc
      961 tttgctcaaa gcggcaatga cattgcctgt gtgattttgg agccttttgt cggcaatatg
     1021 aaccttgtcc gcccgaccga agccttcgtc aaagccctgc gcgaattgac cgaaaaacac
     1081 ggcgcggtgt tgatttacga cgaagtgatg accggtttcc gcgtcgcgct cggcggcgca
     1141 caatcgctgc acggcatcat gcccgacctg accacgatgg gcaaagtcat cggcggcggt
     1201 atgccgcttg ccgcgttcgg cgggcgcaaa gacatcatgg aatgtatttc cccgctgggc
     1261 ggcgtgtatc aggcaggcac cttgtcgggc aacccgattg ccgtcgccgc aggcttgaaa
     1321 acgctggaaa tcattcagcg cgaagggttt tatgaaaacc tgacggcgcg taccgaacag
     1381 ttggttcaag gttttcagac ggcagcggat gcggcgggca tcgagttcac cgccgacagc
     1441 gtgggcggta tgttcggtct gtatttcgcc gcacacgcgc cgcgaaatta tgccgatatg
     1501 gcgcgttcca atattgaagg cttcaaacag tttttccacg ggatgctcga ccgcaatgtc
     1561 gccttcggcc cgtccgctta tgaagcaggt ttcgtttccg ccgcgcatac gccggttttg
     1621 attgatgaaa cggttgcggt tgcggttgaa gtgttcaagg cgatggctgc atgatgtttt
     1681 gacggacagg gtttctctgt tcgatttgtt tggcagattg aagtaagaat gcacaccgcc
     1741 gtcattccca cgcaggcggg aatccagacc tttcagtttc tgtaatgatt gaaaataacg
     1801 gcaggcccga ccttccggat tcccgcctgc gcgggaatga cgggcgtgta tatttttgat
     1861 ttcaatctgc tgtaaaaatg ccgtctgaaa tgcaggattt gcgcttcaga cggcattttt
     1921 tgtttcttat tgcgcttcgg ctctcggggc ggaaatcaga aagtgttcgc ggtagtggcg
     1981 catttcttcg atgctctcca aaatgtcgtc caatgccttg tgcgaaccgc gtttgacgac
     2041 gcttttggca acgggtggat tccagcgttt ggcgagttct ttcagcgtgg aaacgtcgag
     2101 gttgcggtag tggaagtagt tttccagttt cggcatatat ttgaccatga agcgccggtc
     2161 ttggtggatg gagttgccgc acatcggcgt ggcgcgtccg gatacccatt ccgacataaa
     2221 gtccagcagt ttctgttcga cttcggcttc ggtatgcgac gattcgcgca cgcgctgtgt
     2281 cagccccgtc ctaccgtgtg tggcggtgtt ccattcgtcc atattgtcga gcagctcgtc
     2341 gctttggtgg atggcgtaaa cttcggattg cgccaataca ttcaaatccg agtcggtaat
     2401 aatcatcgcg acttcgataa tgcggtcggt ttcgggatta agccccgtca tttccatatc
     2461 gagccagcat aggttgtttt tatcctgcat gatgtttcct ttgcttgtca gcgttttgtc
     2521 ccgctcagtc gcgcccatcc ttcttctgtt tccgcaggat cgagatcgaa ccattggctg
     2581 taaatgccgc tgagttcttc gatttgttcg tccaacaaac cggataacac gatgcggccg
     2641 ccttgtttgg tgcgggcggc aagcatttcg ccgagcatac gtaaagggtt ggcgaggatg
     2701 ttggcgacaa ctacgtcgaa ttgcccttga ggcaggccgt cgggcaggaa gaattgtgca
     2761 tcgacgttgt tctgttcggc gttgtccttg ctggcacgga tggcctgttc gtcaatatcg
     2821 acgccgacgg cagaacctgc gccgagtttg agggcggcga tggtcaggat gcccgaaccg
     2881 cagccgtagt cgaggacgct ttcgccgttt ttgagttgcg tatccagcca tttgaggcag
     2941 aggcgcgtgg ttgggtggct gccagtgccg aaggcgaggc cgggatcgag gcgtaggttg
     3001 acggcagtgc cttcgggggc ttcgtgccaa gagggggtaa tccacaggcg gtcggaaatt
     3061 cggatggggt cgaattgcga ttgcgtgaga cgcacccagt cttggtcttc gatggtttcg
     3121 ccggtgtatg ccaagtcttt taacccgcat tcttgtgcgg cgatttggat gatggcggcg
     3181 gcttcgtcgt gttcgccgaa cagggcgatg actttgctct gctgccagat ttgttcggcg
     3241 ggcataccgg gttcgccgaa aatcgcctgt tcgttttgcg tgccggcgta ggcatcttcg
     3301 atggtggcgg agagtgcgcc gtgttccatc agcgcgtcgg cgaggcgttc ggcgacggca
     3361 tcgttgacgt tgacggtgat ttgttggtag ggcatagcgg gctttcttaa cagaccttcg
     3421 gaatagggtc gtactgtata aggggatggg tgtgtaacgg aaatgccgtc tgaacgcgga
     3481 cgggcggctt cagacggcat atcggcggcg gtttacttgt cctgtttggc tttgcgatct
     3541 tccagccagt gttccaaata gtggatgctg acgccgcctt tttggaaacc cgcatcggcg
     3601 aacaggtcgc ggtgaagcgg cgtattggtt ttgatgccgg ttaccgccag ctcggcgagt
     3661 gcgacgcgca ttttcgccat tgcctgttca cgcgttttgc cgtgtacgca gattttgccg
     3721 atcaggctgt cgtagtacgg tgggatgcgg tagccttggt aaatgtggct gtccacgcgg
     3781 ataccgaagc caccgggcag gtggcagctt tcaatcgggc ccgggctggg gatgaagttg
     3841 tacgggtctt cagcgttgat acggcactcg aaagcgtggc cttcgatttt gatgtctttc
     3901 tgtttgtatt gcagcggcag gccggatgcg atgcggagtt gctcctgcac gatgtccacg
     3961 ccggtaatca gctcggtaac cggatgctca acctgaacgc gcgtattcat ctcgataaag
     4021 aaaaattcgc cgtcttcgta caggaattca aacgtacctg cgccacgata gccgatgcgt
     4081 ttgcaggcgt cggcacaggc gttgccgatt ttggcgcgtt cttcttcagt gatgaacgga
     4141 gccggtgctt cctcgatgac tttttggtgg cggcgttgca gcgaacagtc gcgctcggca
     4201 agatatacgg cgttgccgtg ttcgtcggca atcacttgga tttcgacgtg gcgcggacgt
     4261 tgcagatagc gttccatgta aaccatcggg ttgccgaatg ccgcgcctgc ttctgccttg
     4321 gtcatttcga cagattggag gaggtcttct tttttctcga ccacgcgcat accgcgaccg
     4381 ccgccgccgc cagaggcttt gataatgacg ggataaccga ttttatcggc gattttgagg
     4441 atttcttcgc cgtcgtcggg caatgcgccg tcagaaccgg ggacgcaggg tacgcctgcc
     4501 gctatcatcg cgtgtttggc ggagactttg tcgcccatca ggaggatggt gtcgggtttc
     4561 gggccgataa aggtaaagcc ggactgctcg acctgttcgg cgaaatcggc gttttcggca
     4621 aggaaaccgt agcctgggtg gacagcgtcc gcgcagctta cttcggcggc ggcaataatg
     4681 gcggggacgt tgaggtagct ttgcgcggaa gcggcagggc cgatgcacac ggattcgtcg
     4741 gcgagtttga cgtgcaggct gtctttgtcg gcctcggaat gcacggcgac ggtggcaatg
     4801 cccatttcgc ggcaggcacg gagtacgcgt aatgcgattt cgcctcggtt ggcgattaaa
     4861 acttttttca gcatgatgac tgttcctgtg gttccggcaa cccgtccgta aaaaaaaggt
     4921 gcgggaacgc cgaaggggga aagcatttca gacggcatcg gaagtttatg ccgtctgaaa
     4981 acaggattat ccgatgatga agagcggttc gccgaattcg acgggcgtac cgttttcgac
     5041 caaaatttct ttgaccgtgc cggatttttc ggcttcgatt tcgttcatca gcttcatcgc
     5101 ttcgatgatg cacagcgtgt cgccggcttt aacctgttgg ccgacttcga caaaaggcgc
     5161 ggcattcggg ccgggtgcgc ggtagaacgt gccgaccata ggtgattttt gcgcgtcgga
     5221 caaatcgcgg gcggcaggtg cggcggcggg cgcggatgcc gcaacgggtg cggcggcagg
     5281 cgttacggcc ggcgcggcgg caggtacggg cgcggcgtaa acgggagctg ccgcagctgc
     5341 aatggtacgg gtgatgcgga ctttttcctc gccttcggtt acttcgattt cggcgatacc
     5401 cgattcttca accaaatcaa tcagtttttt taatttgcgc aaatccattt ctgttccttt
     5461 aaaggctgcc ggtgttccgg cgggttgttg cgttggtttt tcagaaaaca gccagcttgc
     5521 ggcaacggcg caaaccgggc ggaatggtgt tcggatacgg ttttcctgtg gtggaaaaca
     5581 accgtgagaa tgtgtttatt ttcccgaagt tgtcggtaaa tgtccagtaa aatatcaaaa
     5641 aacggcggtt tttgggggaa atgtgcagga agtttgattt tgcgcacaag atgccccggg
     5701 tcaagagtgt ttattctaat ctgttggttt ttcgggcaaa gatgccgtct gaaaaagggc
     5761 taaagtgcgt ataatggcgg cttgcccgaa cgagagtgta aaaatggata tttcagattt
     5821 tgattttacc ctgcccgaac acctgattgc ccagcatccg cccgaagtgc gcggcagcag
     5881 ccaccttttg gtcgcgctgc ccgatatgcc gctgcaagac cgggtgttcg gcgatttgcc
     5941 ggattatgtc gaggcgggcg acgttttggt gttcaacaac accaaagtca tgaaggcgcg
     6001 gctgtttggg cagaaagaca gcggcggcag gatcgaagcc ctgattgagc gcgtgttgga
     6061 caaccatacc gcactggcgc acatccgttc gtccaaatcg cccaagcccg gtatggggct
     6121 ggtgtttgaa ggcggcatcc gcgccgtgac ggtcgggcgt gagggcgaac tgttctgcct
     6181 gcgttttgaa ggcggtcaaa ccgtttacga acttttggaa cagaacggac acctgcccct
     6241 gccgccttat atcgaacgcg ccgccgatgc ggacgacgac agccgttatc aaaccgttta
     6301 tgccaaatat cagggtgcgg tcgccgcgcc gacggcgggt ctgcatttta cggaagaact
     6361 tttgcgccgc ctgaaagaca aaggcgcagt aaccgcagaa gtaaccctgc acgtcggtgc
     6421 gggaacgttc cagcccgtgc gtgtggaaaa aatcgaagaa cacaaaatgc acagcgaatg
     6481 gtttgaagtg ccgtctgaaa ccgccgccgc cgttgaggcg gcaaaagcac gggggaacaa
     6541 agtctgggcg gtcggcacga cttccatgcg cgccctcgaa tctgccgcgc gtgcgacggg
     6601 tcgtctgaaa gcggggcagg gcgacaccga tattttcatc acgccgggct accgttttaa
     6661 tgttgtcgac aggctggtta ccaattttca tttgccgaaa tcgacgctgc tgatgcttgt
     6721 cagcgcgttt tcgggtatgg gtcatatccg cgccgcgtat cgttatgcgg tcgaacgcga
     6781 ataccgtttc ttcagctacg gcgatgcgat ggttttgggg cggaacgaag gggtggtacg
     6841 ttagaacagc cccaagtcta ttgggggaag acaagtgccg tctgaacgga cgttcggacg
     6901 gcattctttt gttgatttga atgaaaggaa ctgtatgaca tagattcagg cgcgagaggc
     6961 gagaaactca gtcattgttg atgacgcgca atacgttaaa attcattccg ttttggtcgt
     7021 ataaacatat tacatacgcc atttttttgt attcaaggct gcctgaaaac agtgggcaag
     7081 aatatctaac ccaacgaaaa gctgaaaaag caacaaaaaa taatctgcac cttagtcggt
     7141 tggttagtcc aaccttaggg gtgcagacca ttcaatcttt caggctgcct ttgtttgttt
     7201 ataagataaa tttttagccg aacacttttt ccaagtgtgc ctgataatct gccaagtatt
     7261 tttccacttg
//
LOCUS       DAXHSJ010000108         7202 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000108 DAXHSJ010000000
VERSION     DAXHSJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.108"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(61..145)
                     /locus_tag="ACFM8U_001509"
     tRNA            complement(61..145)
                     /locus_tag="ACFM8U_001509"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(109..111),aa:Leu,seq:caa)
     gene            422..1075
                     /locus_tag="ACFM8U_001510"
     CDS             422..1075
                     /locus_tag="ACFM8U_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC6334733.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1154..1807
                     /gene="hisG"
                     /locus_tag="ACFM8U_001511"
     CDS             1154..1807
                     /gene="hisG"
                     /locus_tag="ACFM8U_001511"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC6334734.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1911..2810
                     /locus_tag="ACFM8U_001512"
     CDS             1911..2810
                     /locus_tag="ACFM8U_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC6334735.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2807..4096
                     /gene="hisD"
                     /locus_tag="ACFM8U_001513"
     CDS             2807..4096
                     /gene="hisD"
                     /locus_tag="ACFM8U_001513"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC6334736.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLKAAFERIPNDVQTALQTAALRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4142..5239
                     /gene="hisC"
                     /locus_tag="ACFM8U_001514"
     CDS             4142..5239
                     /gene="hisC"
                     /locus_tag="ACFM8U_001514"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC6334737.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5236..6153
                     /gene="hisB"
                     /locus_tag="ACFM8U_001515"
     CDS             5236..6153
                     /gene="hisB"
                     /locus_tag="ACFM8U_001515"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC6334738.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6248..7117
                     /locus_tag="ACFM8U_001516"
     CDS             6248..7117
                     /locus_tag="ACFM8U_001516"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC6334739.1"
                     /translation="MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         1879 a         2125 c         1723 g         1475 t
ORIGIN      
        1 gtcttatcta tttcattaaa cagcaataga caaaaaaaat aaccgctcca aaagcggttg
       61 tggtgcccag ggtcggactc gaaccgacac accttgcggc gggggatttt gagtcccctg
      121 cgtctaccaa tttcgccacc tgggctggtg aagaagtcgt cattataatg gcttttgaaa
      181 ttctgtaaac cttttttttg aaattatttt atctgttttt attttatttt tgattttaaa
      241 tagaattttt attattttaa tcttactgtt ctttccgctc caaagattct gtatgattcg
      301 gcaattcctg ccgtgcagac aacgtaaaaa aatactacat taaatctgcc aaacgcgtta
      361 agatggaaat attcaaattc cgtacgaatc aggttttgct atttattctt gggagattgt
      421 catgttttcc gtaccgcgtt cctttttgcc gggcgttttc gtacttgccg cgcttgccgc
      481 ctgcaaacct caagacaaca gtgcggcgca agccgcttct tcaagtgcat ccgcgtcagc
      541 tgcggaaaat gcggcaaagc cgcaaacgcg cggtacggat atgcgtaagg aagacatcgg
      601 cggcgatttc acgctgaccg acggcgaagg caagcctttc agcctgagcg atttgaaagg
      661 caaggtcgtg attctgtctt tcggctttac gcactgtccc gatgtctgcc cgacagagct
      721 tttgacgtac agcgacacgt tgaagcagtt gggcgggcag gctaaggacg tgaaagtggt
      781 gttcgtcagc atcgatccgg aacgcgacac gcctgaaatc atcggcaagt atgccaaaca
      841 gttcaatccg gactttatcg gtctgacggc aacgggcgac caaaacctgc cggtcatcaa
      901 gcagcaatac cgcgtggttt ctgccaaagt caatcaaaaa gacgacagcg aaaactattt
      961 ggtcgaccac tcttccggtg cgtatctcat cgacaaaaac ggtgaggttg ccattttctc
     1021 gccttacgga agcgagccgg aaacgattgc tgccgatgta aggaccctgc tctgataaaa
     1081 ccgtatgccg tctgcaccgt cggcgcctgt tcagacggca ttattgtttc aaccgacaaa
     1141 ggacatccac accatgcagg ataatgcttt gaccatcgcc ttatccaagg ggcgcatttt
     1201 tgaggagacg ctgccgctgc ttgccgctgc cggcattgtt ccgactgaag agcctgaaaa
     1261 atcgcgcaag ctgattatcg ggacgaacca tgaaaacatc cgccttgtca ttgtccgcgc
     1321 aaccgatgtg ccgacttatg tccgctacgg cgcggcggac ttcggcattg cgggcaaaga
     1381 cgtgctgatc gaacacggcg gcacggggct ttaccggcct ttggatttgg agattgccaa
     1441 gtgccgcatg atggttgctg tgcgtaaagg gtttgattac gaagcagctt cgcaacccgg
     1501 atgccgtctg aagattgcca caaagtatcc tgaaatcgcg gcatcccatt ttgccggcaa
     1561 gggtgtccat gtggacatta tcaaactgta cggctcgatg gaacttgcgc cgctggtcgg
     1621 cttgagcgat gcgattgtgg acttggtttc gacgggcaac accttgaagg caaacggctt
     1681 ggaagcagtc gaacacatcg tcgacatttc cagccgcctg gtggtcaaca aggctgcttt
     1741 gaaaacgaaa tacgcgctgc tggagccgat tattcaggcg ttcggcggcg cagtgaaggc
     1801 gaagtaagca tccatttgaa taaagatgcg ttttcagacg accctatccg ttcccgccga
     1861 caggtcgtct gaaaatatca ccggcagtaa actgtatagg agaagttaaa atggttgcaa
     1921 aaataaaaaa attctcagat tcaacccttt ccgttttgaa taacggcgag cgtcggtttt
     1981 atgtctattg tctgaccgac ctgaaaaaag acaaaatcct ctacatcggc aaaggctgcg
     2041 gtaatcgtat cttcgagcat gaatgggttg ctagtcgttc acaagaccca gtctccggcg
     2101 agattatcga tcggaaactc aaagccatct ccaaatgcaa gaaactcggt cgctatatca
     2161 tcagctatca tctgactgaa gtcgaagcac tcgccgccga atctgcctta attcattttg
     2221 ttaaatctgt cttgggtaaa aaactcaaaa ataaaattgc cgggcatggt ccgggtggta
     2281 ttagcgtaga agaactagat cgccgctttg gattctcttc tctcccactt aacgagatta
     2341 accccgacgg gctgattctc gccatcaaaa tccacaatgc tttcgattta gatactgacg
     2401 aagaattaga ctaccttttc gacaaccaag acgatgccaa cctcaaatcg cgtacgttgg
     2461 gcaactgggt tatcggtaaa gatgttgctt caaaagtgaa atacgttatc ggcgttcaca
     2521 ccggtctgca aaacgctgtt gtcagtgcat acgaagtgga cggttttgaa acaatggttg
     2581 aggaaaccaa aaacggtaga aaacaatccc gttaccgttt ccgcactacc tctcgtagcg
     2641 aagaggtatt agccaaactc ggtctgcaac aaaaatgcct gcccgaattg aagtttggta
     2701 gcgggggaga aaaagcgtat atcagaccca aaacagagac agaaactgaa caagagaata
     2761 ttcagacgac ccccaatcca aaaataaaaa aggaaaaaac caaatcatga aaaaactcaa
     2821 cacccaatcg cccgatttcc aagccggact caaagccctg ctggcttttg aaaccgcgca
     2881 aaaccccgaa accgaacgca tcgtcgccga catttgcgcc gacgtgcaaa agcgcggcga
     2941 tgcggctttg attgaataca ccaacaaatt cgatcagaca aacgctaaaa gcatcgatga
     3001 tttaatactt acgcaagccg atttgaaagc ggcgttcgag cgcattccga acgacgttca
     3061 gacggcattg cagaccgccg ccctccgtgt cgaaagctac caccaacgcc aaaaaatgga
     3121 atcgtggagc tacaccgatg aagacggcac gctgttggga caacagatta ccccgcttga
     3181 ccgcgtcggc atttacgtcc ccggcggcaa ggcggcatat ccgagttccg tcatcatgaa
     3241 cgccatgccc gcccacgtcg caggcgtgaa agaaattatt atggtcgtgc cgacaccgaa
     3301 aggcgaacgc aacgacatcg tgcttgccgc cgcatacgtc gccggcgtaa ccaaagtctt
     3361 caccgtcggc ggcgcgcagg cggttgccgc cctcgcctac ggcacggaaa ccatccccca
     3421 agtcgataaa atcaccggtc cgggcaacgc cttcgtcgcc gccgccaaac gccgcgtgtt
     3481 cggcgtggtc ggcatcgaca tggtggcggg gccgtctgaa atcttggtca tcgccgacgg
     3541 cacgacacct gccgattggg tggcgatgga tttgttcagc caggccgaac acgacgaaat
     3601 tgcccaagcc atcttaatcg gcacgtcgca agcgtatctc gacgaagtag aagccgctat
     3661 ggacaggctc atcgaaacca tgccgcgccg cgacatcatc gaagcctcgc tcggcaacag
     3721 gggcgcgatg atactcgcca aagacttgga cgaagcctgc gaaatcgcca actacatttc
     3781 ccccgaacac ttggaattgt cagtcgaaaa tccgcaggaa tgggcgaaaa aaatccgcca
     3841 cgccggcgcg attttcatgg gacgctacac cggcgaaagc ctcggcgact actgcgccgg
     3901 cccaaaccat gtgttgccca ccagccgaac cgcccgcttt tcctcgcctt tggggacata
     3961 tgatttccaa aaacgctcca gcctgattca ggtttcggaa cagggcgcgc aaaaattagg
     4021 cgaaaccgcc agcgtgctgg cacacggcga aagcctgacc gcccacgccc gcgcggcaga
     4081 gttccgtatg aaataatgcc gaaacggcgt acaggcatat tccaaccatt aaggaaacac
     4141 gatgaaatcc gtccgctcct tcatccgcga cgacatacaa gctatgtcgg catatcagat
     4201 tgccgacgtt ccgcccggct ttgccaaact cgattcgatg gaaagtcccg tccacccttt
     4261 tgccggacat gaaacgctgt tgcaggaatg gcaggcacgg cttgccgccg cgcccatcca
     4321 tctttacccc aatccctccg gcagcggttt acaggaagca ttacgttcgg cgttcgacat
     4381 tcccgactgc gccgacatcg cgctgggcaa cggctcggac gagctgatac agttcatcac
     4441 gatgctgacc gccaaaccgg gcgcggcaat gttggcagcc gaacccagtt tcgtcatgta
     4501 ccgccacaac gccgcgctgt acggcatgga ttatgtcggc gttccactga acggagattt
     4561 caccctcaac ctgcccgccg tcctcgaagc cgtcaggaag caccgccctg ccctgacctt
     4621 tatcgcctac cccaacaacc ccaccggcgt atgtttcacg cgtgccgaaa tcgaagccgt
     4681 catcgaagct tcagacggca tcgtcgtcgt cgacgaagcc tacggcgcat tcaacggcga
     4741 cagcttcctg ccgcaggcgg gcagcattcc caacctcatc gtcatgcgta ccgtcagcaa
     4801 aatcggtttt gccggactgc gtatcggcta tgcggcaggc tgtcccgaag tcatcggcga
     4861 actgcaaaaa atcctgccgc cctacaatat gaaccaactg agcctgacca ccgccaaact
     4921 cgccctgcaa cactacggca tcatctctgc caacatcgac agcctgaaaa acgaacgcga
     4981 acggatgttc gccgaattgg gcaaaatatg ccgtctgaac acctttccaa gtcaggcaaa
     5041 cttcattacc atacgcgtac ccgatgccga tttgttgttt gacacgctca aacaaaaccg
     5101 catcttggtt aaaaaactgc atggcgcgca cccgcttttg gaacactgcc tgcgcattac
     5161 cgtaggcagc cccgcacaaa acgatgccgt tctcaacatc attcgccaac tttactgcca
     5221 accaacggat ttcctatgaa tttgactaaa acacaacgcc aactgcacaa ctttctgacc
     5281 ctcgcccaag aagcaggttc gctgtccaag ctcgccaaac tctgcggcta ccgtaccccc
     5341 gtcgcactct acaaactcaa acaacgcctt gaaaagcagg cagaagaccc agatgcacgc
     5401 ggcatccgtc ccagcctgat ggcaaaactc gaaaaacaca ccggcaaacc caaaggctgg
     5461 ctcgacagaa aacaccgcga acgcactgtc cccgaaaccg ccgcagaaag caccggaact
     5521 gccgaaaccc aaattgccga aaccgcatct gctgccggct gccgcagcgt taccgtcaac
     5581 cgcaatacct gcgaaaccca aatcaccgtc tccatcaacc tcgacggcag cggcaaaagc
     5641 aggctggata ccggcgtacc cttcctcgaa cacatgatcg accaaatcgc ccgccacggc
     5701 atgattgaca tcgacatcag ctgcaaaggc gacctgcaca tcgacgacca ccacaccgcc
     5761 gaagacatcg gcatcacact cggacaagca atccggcagg cactcggcga caaaaaaggc
     5821 atccgccgtt acggacattc ctacgtcccg ctcgacgaag ccctcagccg cgtcgtcatc
     5881 gacctttccg gccgccccgg actcgtgtac aacatcgaat ttacccgcgc actaatcgga
     5941 cgtttcgatg tcgatttgtt tgaagaattt ttccacggca tcgtcaacca cagtatgatg
     6001 accctgcaca tcgacaacct cagcggcaaa aacgcccacc atcaggcgga aaccgtattc
     6061 aaagccttcg ggcgcgccct gcgtatggca gtcgaacacg acccgcgcat ggcaggacag
     6121 accccctcga ccaaaggcac gctgaccgca taaaaaacca taccgtctga aacaccctca
     6181 ggcttttcag acggtatcgg aacagataag attacactac actacaaaca gaaaaggagt
     6241 aaacatcatg tccgcaaacg aatacgcaca aatcggctgg ataggcttag ggcaaatggg
     6301 tctgcctatg gtaacgcggc tcttggacgg cggcatcgaa gtcggcgtat acaaccgctc
     6361 gcccgacaaa actgccccca tctccgccaa aggcgcaaaa gtttacggca acaccgccga
     6421 actcgtccgc gactatcccg tcattttcct gatggtttcc gactatgccg ccgtgtgcga
     6481 catcctgaac ggagtccgcg acggattggc cggcaaaatc atcgtcaaca tgagcaccat
     6541 ctccccgacc gaaaacctcg ccgtcaaagc acttgtcgaa gccgcaggcg gacagtttgc
     6601 cgaagcaccc gtttccggat cggtcgggcc cgccaccaac ggcacgctgc tgattctgtt
     6661 cggcggcagc gaagccgttt taaacccgct gcaaaaaata ttttccctcg tcggcaaaaa
     6721 aaccttccat ttcggcgatg tcggcaaagg ttcgggcgcg aaactcgtct tgaactcgct
     6781 cttgggcatt ttcggcgaag cgtacagcga agcgatgctg atggcgcggc agttcggcat
     6841 cgataccgac accatcgtcg aagccatcgg cggctcggca atggactcgc ccatgttcca
     6901 aaccaaaaaa tccctgtggg caaaccgcga attcccgccc gccttcgccc tcaaacacgc
     6961 ctccaaagac ctcaacctcg ccgtcaaaga gcttgaacag gcaggcaaca ccctgcccgc
     7021 cgtcgaaacc gttgctgcca gctaccgcaa agcagtcgaa gccggctacg gcgaacagga
     7081 cgtttccggc gtttacctga aactggcaga acactgactt ccctttccaa acaatatgcc
     7141 gtctgaaata ttttcagacg gcatttttat cgccccacgc ttaaaatcag tcccgattat
     7201 ga
//
LOCUS       DAXHSJ010000109         7176 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000109 DAXHSJ010000000
VERSION     DAXHSJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.109"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(90..305)
                     /locus_tag="ACFM8U_001517"
                     /pseudo
     CDS             complement(90..305)
                     /locus_tag="ACFM8U_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(334..663)
                     /locus_tag="ACFM8U_001518"
     CDS             complement(334..663)
                     /locus_tag="ACFM8U_001518"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6334740.1"
                     /translation="MGSLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(834..1061)
                     /locus_tag="ACFM8U_001519"
     CDS             complement(834..1061)
                     /locus_tag="ACFM8U_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HFC6334741.1"
                     /translation="MLKTLTFAALHFTTALSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YSGLTKTSTALPRLAVLFVLSAASSPCPDFC"
     gene            1184..2137
                     /locus_tag="ACFM8U_001520"
     CDS             1184..2137
                     /locus_tag="ACFM8U_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6334742.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVHD
                     AHVVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAEAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHASAVREGYVSFEDAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(2212..4269)
                     /gene="metG"
                     /locus_tag="ACFM8U_001521"
     CDS             complement(2212..4269)
                     /gene="metG"
                     /locus_tag="ACFM8U_001521"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC6334743.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGCLKDVADSALLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPKVAEN
                     AAKFLNLEAITWANTRETLGKHSINKYEHLMQRVEQKQVDDLIEANKQSIQTTPAPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(4385..6223)
                     /gene="glmS"
                     /locus_tag="ACFM8U_001522"
     CDS             complement(4385..6223)
                     /gene="glmS"
                     /locus_tag="ACFM8U_001522"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC6334744.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLSYHVALARGTDVDKPRNLAKSVTVE"
     gene            6505..7023
                     /locus_tag="ACFM8U_001523"
     CDS             6505..7023
                     /locus_tag="ACFM8U_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334745.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKKE
                     KGNYFLNKINVFTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARKALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
BASE COUNT         1515 a         1710 c         2149 g         1802 t
ORIGIN      
        1 gaaaaaacag aaaccgctcc gccgtcattc ccgcgcaggc gggaatccag cctcgtcggt
       61 acggaaactt atcgggtaaa aaggtttctc tagtttggtg tcgattttct tgtcggtgct
      121 gttgacggca ggtgcttggt ctcgatcttc ttgtcgctgt cggcggtaac gtcgtcggag
      181 ttggtgccgg tgccggtgcg cgtaaccggc tgtccgcaga accattttac cgaaccgtct
      241 tgacgcttgg cccacaggga gagttttttg tctttgattt cgttgtttac gttgcttgaa
      301 gccattatgt cagacggtat tgcccgggca gctttattcg tacactttca gcagctcgac
      361 ttcaaatatc aaagtggcgt gcggaggaat cacgccgccc gcgccgtgtg cgccgtagcc
      421 catttccgaa gggatggtca gcttgcgttt gccgccttcc ttcatgccgc cgaagccttc
      481 gtcccagcct ttgatgactt gtccgacacc gagcgtaatg gtcagcggct ggcggcggtc
      541 gaggctggag tcgaatttgg tgccgttttc cagccaacct gtgtaatgca cggtaatctc
      601 tttgccttta actgcttctt ttccgaagcc ttcctgcaaa tcttcaataa tcaggctgcc
      661 catatctgtc ctttcgttgc ttgttggtca aaacggcaag ggtaacatac cgtccggcga
      721 agtcaaatac cgccaatcgt cagccgcacc ggtgcagcgg gaacagcagt tgttgtttgc
      781 ccgttttgtt tttttcgtaa cggttccatg ctttttcatg gaaatatagt ggattaacaa
      841 aaatcagggc aaggcgacga agccgcagac agtacaaata gtacggcaag gcgaggcaac
      901 gccgtactgg tttttgttaa tccactatag aaaacgacgg tgttgattag gggttcgacc
      961 agcgcgaccg cgccagatac gcctatactg cccgtcagca cataggttac actgagggcg
     1021 gtggtgaaat gcagtgcggc aaaagtcagg gttttaagca tcatcctctc ccgaattgaa
     1081 aatttacgga gagatgataa agattatcat caggccgtgc cggtttaaat ttgctattta
     1141 ctgttagtgt aaataaatca atttttaagt aaggatagaa atgatgaatc acaaaaagat
     1201 agtcgttctg gatgcggata ctttgcccgg cagggttttt cattttgatt ttccgcacga
     1261 gctcgcagtt tacggtacga caggtgcgga tgaaacggca gaacgggtgc acgacgcaca
     1321 tgttgtcatt accaataagg taatgatttc cgccgacatt attgcggcta atccgcagtt
     1381 ggagctgatt gccgtcagtg cgaccggtgt gaacaatgtc gatattgggg cggcggaggc
     1441 ggcgggcgtt gcggtatgca atgtccgcgc ctacggaaac gaatcggttg cggaacacgc
     1501 ctttatgctg atgattgcgt taatgcggaa tctgcctgcc tatcagcgtg atgttgcggc
     1561 cggtttgtgg gaaaagtcgc cgtttttctg ccattacggc gcgccgattc gggatttgaa
     1621 cggcaaaacg ctggcggttt tcggacgcgg caatatcgga cggacgcttg ccggatacgc
     1681 gcaggcattc ggtatggggg tggtgtttgc cgaacacaaa cacgcgtccg ctgtgcgcga
     1741 aggctatgtt tcctttgaag atgcggtacg ggctgctgat gtgttgtcgc tgcactgtcc
     1801 gctaaacgcc caaactgaaa atatgatagg cgaaaacgaa ttgcggcaga tgaagcctgg
     1861 cgcggtttta atcaattgtg ggcgcggcgg gctggtggat gaaaacgcgc tgcttgccgc
     1921 actcaaatac gggcagatcg gcggggctgg tgtcgatgtt ttgacggagg agccgccaag
     1981 gggcggcaat cccctgctga atgcacgact tcccaatctg attgttacgc cgcataccgc
     2041 gtgggcgagc cgcgaggctt tggacaggct gttcgatata ttgttggcga acattcacgc
     2101 ctttgtgaaa ggcgaggcgc aaaaccgggt agtttgagct tgtcgggata acgaaaaaca
     2161 atgccgtctg aacgcccaaa gggttcagac ggcatttttt gagatttcta cttaaccgac
     2221 tttgtcgccc ggttgcgcgc ctgcatccac atccaagagc ttcagtttcc cgtctgccgt
     2281 ggcagcggag agaatcatgc cttcggatac gccgaatttt gccattttgc gcggggcgaa
     2341 gttggcgacg gcgatgacca tgcggccgtt caattcggca ggatttgggt aagacgcggc
     2401 gatgccggag aagatgatgc gtttttcaaa accgaaatcg aggtcgaatt ttaagagttt
     2461 ggtgctgcct tcgacggctt cgcagttcaa tactttggcg acgcgcatat cgattttcat
     2521 aaagtcgtcg aagctcgcct gttcggcgac tttttcgtat ttgctctctt cggcggcagg
     2581 cgcgggggtc gtctgaatgc tttgtttgtt ggcttcgatt aaatcgtcca cttgtttttg
     2641 ctccactcgt tgcattaaat gttcgtattt gttgatggag tgtttgccca aggtttcgcg
     2701 tgtatttgcc caagtgatgg cttccaaatt caggaatttg gcggcatttt cggccacttt
     2761 cggcaacacg ggggcgaggt aggcggtcag catggtaaag gcgttgatga gttcgctgca
     2821 tacttcgtgc aggcgttcgt cttggccttc ttgtttggcg agttcccaag gtttgttggc
     2881 atcaacgtat tcgttgacaa tgtctgccaa ggccatgatg tcgcgcaggg ctttggcgta
     2941 ttcgcggctt tcgtagcatt cggcaatggc ttcgctttgt gcggtcagtt ttgccagcaa
     3001 tgcgctgtcg gcaacgtctt tcagacagcc ttcaaagcgt ttggcgatga aacctgaggc
     3061 gcgggcggcg atgttgacgt atttgccgac gaggtcgctg ttgacgcggc tgataaagtc
     3121 ttgcaggttc aaatcgatgt cttcgatttt gctgttgagt ttggcggcga tgtagtagcg
     3181 catccactcg gggtttaaac cttgttccag ataggatttg gcggtgataa acgtgccgcg
     3241 cgatttggac attttttgtc cgtctacggt caaaaagccg tgtgcgtaca cgccggtcgg
     3301 ggcgcggtgg ccggagaaat gcagcatggc aggccagaac agggcgtgga aatagaggat
     3361 gtctttgccg atgaagtggt acatctcggt ttggctgtcg gctttgaagt attcgtcaaa
     3421 atcgacgccg atgcggtcgc acaggttttt aaacgacgcc atgtagccga cgggcgcgtc
     3481 cagccagacg tagaagtatt tgcccggcgc gtcggggatt tcaaaaccga aatacggcgc
     3541 gtcgcgggaa atatcccagt cggacagggt ggtttcttca ccttcgccca gccattcttt
     3601 cattttgttg agggcttcgg cttgcagatg gggcttgccg tcgtgcgggt tgttgccgga
     3661 agtccatgct ttgaggaagt cggcgcattc tcccagtttg aagaagaagt gttcggattc
     3721 gcgcaattcg ggtttggctc cggatacggc ggaatacggg ttgatcagtt cggtcgggga
     3781 ataggtcgtg ccgcagactt cgcagttgtc gccgtattgg tcttgggcgt ggcatttcgg
     3841 gcattcgcct ttgacgaagc ggtcgggcag gaacatttgt ttttcggggt cgaaaagctg
     3901 ctcgatgacg cggctttcga ttttgccgtt ggctttcagt gcgcggtaaa tgtcttggga
     3961 aaactgtttg ttttcagggg aatgggtgct gtaataattg tcgtagccga tgaaaaaacc
     4021 ggtaaagtcg gcgaggtgtt cttcgcgcac tttggcaatc atgtcttcgg gcgcaatgcc
     4081 ttgtttttgc gcggcaagca tcacgggcgt gccgtgggtg tcgtcggcgc agcagtagtg
     4141 gcactcgtgg ccgcgcagtt tttgaaagcg cacccaaacg tcggtttgga tgtgttcgac
     4201 catgtggccg aggtggatgc tgccgttggc atagggcagg gcggaggtaa cgaggatttt
     4261 gcgtgtcata ttgtgctttg caaacaaagg gtaaaggcgg attataccgc aaatcaaacg
     4321 gggaaatgcc gtctgaagcc tgaaaaatca ggcttcagac ggcatgttcg caaacaatcc
     4381 ggacttattc gaccgtaacg gatttcgcca ggttgcgcgg tttgtctaca tcggtgccgc
     4441 gtgcgagggc gacatggtag gaaaggagct gcacggggat agtatgcacg acgggggaca
     4501 gtttgccgac gtggcgcggt gcgcggataa cgtgcacacc ttcggtggca ttaaaattgc
     4561 tgtcgaggtc ggcaaagacg aaaagttcgc cgccgcgcgc gccgacttcc tgcatattgg
     4621 ctttgacttt gtccaacagg ctgtcgttgg gtgcgatgac gacgacgggc atgttttcgt
     4681 ccaccaaggc aagcggcccg tgcttcagtt cgccggcagg ataggcttcg gcgtggatgt
     4741 aggtgatttc cttcagtttt aacgcgcctt cgagggcaat cgggtaatgg atgccgcgcc
     4801 ccaaaaacag cgcgctggtt ttcttggcaa actgttgcgc ccaggcggca atttgcggtt
     4861 cgaggtttaa agcgtgctgc acgctgccgg gaagctggcg gagttcttcg gtgtaacgtg
     4921 cttcgtcttc ttcggaaacc aaaccgcgca ctttcgccag cgttaccgcc aaaccgaaca
     4981 gcgcaaccag ctgcgtggta aatgctttgg ttgaggcgac gccgatttcc gcaccggcac
     5041 gggtataaag cacgaggctg ctttcgcgcg gcagggcgga ttccatcacg ttgcagatgg
     5101 agaggctgtg gcggtgtccc aaggattttg cgtatttcaa cgcctccatc gtgtccagcg
     5161 tttcgccgga ttgggaaatg gtaatgacca gttgatccgg atcggcaatc acgctgcggt
     5221 atcggtattc gctggcgatt tcgacatcgg ctgggatttt ggcgatggat tccaaccaat
     5281 atttggcggt cagtgcggcg taataggacg tgccgcaggc aaggattttg acgctgcgga
     5341 tgctttcaaa cacgcttttg gcgtttttgc cgaagttttc ggggatgaag ccgccgtcga
     5401 ggaaaacctc cgccgtgtct gcaatcgcgc gcggctgctc gtggatttcc ttttgcataa
     5461 agtggctgta cggtcccagt tccaaagagg cgagcgagag ttcggatacc ttgactttgc
     5521 gttcggcagg caggccgttt ttatcggtca gccgtttgat gccgtctgaa gccagcagcg
     5581 cgatgtcgcc gtcttcgagg tacgccacgc ggcgcgtaaa ggcgatgacg gcggatacgt
     5641 ccgaagcgat aaaggtttca tcgtcgccca aagcgaccaa aagcgggcag cccatacgcg
     5701 ccacaactaa ttcatcaggc ttgtcttggg caataaccgc gatggcgtat gcgccgtgga
     5761 aacgtttgac cgcttcttgt accgcttcaa acagcctgcc gccgttttgc gcgtattcgt
     5821 gattgatgct gtgtgcgatg acttcggtat ccgtttgcga ttcaaaacgg tatcccaaac
     5881 cttccaaacg tttgcgttcg ctttcaaagt tttcgatgat gccgttgtgt acgaccgcaa
     5941 tcataccgcc gctgatgtgc gggtgggcgt tcggctcagt aacgccgccg tgtgtcgccc
     6001 aacgcgtatg tccgatgccg atgccgccgc tgatgccttt ttcgcgtgcc gcgtcctcca
     6061 taagctgcac gcgtccgacg cggcgcacac gtttgatttt gccgtcggtg ttgacggcaa
     6121 tgcctgatga gtcataaccc cggtattcga ggcgtttaag gccgtcggtc agaaaatcga
     6181 cgacgttgtg atgggcgcgg atggcgccga cgataccgca cataactgtt ccttagtatc
     6241 cggttgaaaa aaaacaggcg cggacggctt ccgtgccgca ccttcctctt cggattataa
     6301 accgcctccc gcgccggaaa acagcaaaat gccgtctgaa ggcttgggct tgctcaaaaa
     6361 agggagggat ttccccgcct gaccggggtg ggcgttcaga cggcattgcc tgctgctggt
     6421 ttatagtttt tgcaaatcaa tattgacaag ctgaaaaaaa aaaacaatat actcgctcgg
     6481 tcttaatgtt aacggagtat ggaaatgaaa caaatgcttt tagccgtcgg cgtggtggca
     6541 gtgttggcgg gctgcggcaa ggatgccggc ggttacgagg gttattggcg cgaaaagtcg
     6601 gacaaaaaag agggcgtgat tgccgtcaaa aaagaaaaag gcaattactt ccttaataaa
     6661 atcaacgtat ttacaggcaa ggaggagtct ttgcttttgt ctgaaaaaga cggcgcgctt
     6721 tcgataaaca cggggatagg ggaaatcccg atcaaacttt ccgacgacgg gaaagagctg
     6781 tatgtcgaac gcaggcagta tgtcaaaacc gatgcggcga tgaaggacaa aatcatcgcc
     6841 catcagaaaa agtgcggaca aacggcacag gcatacctcg acgcgcgaaa ggcgttgccg
     6901 tcaaaccaaa cgtatcagca gcatcaggcg gcgatcgagc agttgaaacg gcggtttgaa
     6961 gccgagttcg acgaattgga aaaagaaatc aaatgcaacg gcaaaccgac attgttgttt
     7021 tagcgtagga caaccgggag gatgccgccg tccgaatcgg atgtgtggtt ttcgtaccgg
     7081 tacgggcggg caggaatgtc cgcttttttt gttcggacgc gtttgaatat ccgtccgatt
     7141 ccgtccgttt gcaaagggta tttttgttcg ggcgga
//
LOCUS       DAXHSJ010000110         7135 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000110 DAXHSJ010000000
VERSION     DAXHSJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.110"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(16..771)
                     /locus_tag="ACFM8U_001524"
     CDS             complement(16..771)
                     /locus_tag="ACFM8U_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC6334746.1"
                     /translation="MKYVRLFFLGAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(896..2055)
                     /gene="nirK"
                     /locus_tag="ACFM8U_001525"
                     /pseudo
     CDS             complement(896..2055)
                     /gene="nirK"
                     /locus_tag="ACFM8U_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
     gene            2429..4684
                     /locus_tag="ACFM8U_001526"
                     /pseudo
     CDS             2429..4684
                     /locus_tag="ACFM8U_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
     gene            4863..5396
                     /locus_tag="ACFM8U_001527"
     CDS             4863..5396
                     /locus_tag="ACFM8U_001527"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFC6334747.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5459..6646)
                     /locus_tag="ACFM8U_001528"
     CDS             complement(5459..6646)
                     /locus_tag="ACFM8U_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC6334748.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTAPASNNAPSAPSHPVA
                     KARTVYPKPPRHSNSADNLLRIVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRR
                     FQRFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHG
                     TLFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTV
                     DVPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLN
                     TLYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHI
                     ADKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVAD
                     ISE"
     gene            complement(6686..7090)
                     /locus_tag="ACFM8U_001529"
     CDS             complement(6686..7090)
                     /locus_tag="ACFM8U_001529"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC6334749.1"
                     /translation="MTAQPCPICTAQNEDVLLQTPKLRVIAVHNDSGSPAFCRVIWHE
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1584 a         1741 c         1999 g         1811 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgtgca acggaagccc aagttgtgca agacatattt
       61 ggattgcagg ctggtgcgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      121 cgacccgata gacgcgccgc tgcaaaacat ttgcgcgttg gcattgccgg aagaaagcag
      181 gctgctgttg aaatcttccg tccattccca aatcagcccg tgcatatcat aaacgcccca
      241 gtagttcggg cggcctttgc cgacatcgtg caggcctttc cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      421 ggcgttggcg gcaaaccagg aaacatttgt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      541 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagccgccgc
      721 ttgagtgccg gcgagtgccg cgccgaggaa aaataaccgg acatacttca taaagcctcc
      781 tgacaggcgg ttaaaatcaa tcttccgaaa ggaaagattg gttgttaaaa aaccactgcc
      841 gtgcgtaatg aagtacagcg gcagtggttc gtcccgctaa tgacgggtat ccaatttaat
      901 aaacgctttt ttcggatgca gaggctgccg gggcagaagc tgcgggagcg gaagcagcag
      961 gagctgcacc gttaccggcg taagcggtat cactcaattt ttgagtcatg atttcagggt
     1021 tttccgcacc ctctactttc aattggccca acgcgccttt gttgaatgcg cggaagatgg
     1081 agtggtcgac caaagtgtag ctgcccggga tgtcgacttt gaattcgacg atggcggaac
     1141 cgccggcagg aacgatggta ctttgtacgt tttcgttaat cagtttgccg ccttcaacat
     1201 aaactttgtc gaagatttcg ccgatgacgt ggaaggaaga cactaggttc ggaccgccgt
     1261 taccgacgta catacgcacg gtttcgcctg ctttggcttt cagcgcgtta tcgccggcga
     1321 tagaacctac atgaccgttg aatacgacgt attcaggctg ttcggcaacg gctttgtcca
     1381 tatcgaacgg ttgcaggcct tgcgcgcctt ttttgccttt ggtgtagaag tcgccttgga
     1441 cgatgtagaa ctctttatcc actttcggca gaccttcttt aggctcgacc aaaatcagac
     1501 cgtacatacc gttggcgatg tgcataccga ccggcgcgac ggcgcagtgg tagatgtaca
     1561 gacccggttg cagggctttg aagctgaatg tggaagtgcg gcccggggcg gtaaaggttg
     1621 cggctgcacc gccgccctga ccggttgcgg cgtggaagtc gacgttatgg ggaacggtag
     1681 aagacgggtt gttggaaaat tccacttcaa ccgtatcgcc ttcgcgtacg cggatcatgc
     1741 gacccggaac gtcgccgtca aatgtccagt agcggtattc cacaccgtct tccatggtca
     1801 tggtttttcg acagtttcca tttttacgcg gactttggca ggatagtcgc ggtcgattgc
     1861 gggaggtact tcaggagcgt gggtggtaac tgcatcgata acgggcagtt cgcctgtcgg
     1921 cgtttcggcg gcggtttgtg cggcggaact tgcggcttct gcggaagcgg caggggcttg
     1981 cgcggcaggt tcgccgccgc aggcggctaa tgcgaataag gaagcaatca ttgcagctaa
     2041 ggcttggcgt ttcataatgt tttccttttg taagaaaagt agaggaattg gttaagaact
     2101 tggtgtgatt atgcgtttgc cccattgggg gcattttgat ttaagtcaaa taattcataa
     2161 aatatgaact taaaatgata cttctaatac tttatagcta ctaaaaatta caatgaatag
     2221 gctgatttgc ttaagggctt cggcaaatca ggcaaaggca gggtagttgc cgactaaaga
     2281 aaatgatagt tattatcata tatgtatttg ttttatatgt gagtttcttt gaacaaatgt
     2341 ttatttgtgc gctaaaccgt gctacaatct ttaacattca tattctgtga attttaaaca
     2401 accactttta ccaaaaggag ctctaaaaat gggacagtac aagaagctat ggtacttgct
     2461 gtttgccgtt ctggcggtat gctttaccat tcttggctat atgggcagcg aggtttacaa
     2521 aaaagccccg ccttaccctg aacaggtcgt ttccgcatca ggcaaagtgc tgatgacgaa
     2581 agacgatatt ttggcaggtc agtctgcgtg gcagagtacc ggcggtatgg aagtcggttc
     2641 gattctgggt cacggcgcat atcaggctcc ggactggacg gccgactggc tgcatcgtga
     2701 gttggttgca tagttggatt tgaccgcgca acagacttac ggcaaaaaat tcgatgaagt
     2761 ttcccctgaa gaacaagccg ttctgaaaac ccgactggct gacgaatacc gcaaccaaag
     2821 ccgtattaaa gaagacggca gcgtcgtcat cagcgatacg cgcgtgaaag ccatcgaaag
     2881 catcctgcct tattaccacg gtgtttacgg cgatgatccc gcgctgcaaa ccacccgcga
     2941 acactttgca atgaaaaaca acacattgcc aagccaagaa gcgcgtgaaa aactgttcga
     3001 cttcttcttc tggacttctt ggtctgcttc gaccaaccgt cctgacgaga cgttcactta
     3061 taccaacaac tggccgcacg agcctttgat caacaacgta ccgaccactg aaaactatat
     3121 gtggtcattc accagcgttg ttcttctgct gatgggcatc ggtttgttga tgtggggtta
     3181 ttccttcctg accaaacacg aggaagtgga agtgccgtct gaagacccga tttccaaaat
     3241 ccaactgacc ccttcacaaa aagcattggg caaatacgtc ttcctgacgg ttgccctgtt
     3301 tgtggtacaa gtattgctgg gcggtctgac cgcgcactac accgtcgaag gccagggttt
     3361 ctacggcatc gacgaagcgt tgggctttga aatgtccgac tggttccctt atgccctgac
     3421 ccgtacttgg cacatccaat cagccatctt ctggattgca accggctttt tgacggcagg
     3481 tttgttcctt gcgccgattg tcaacggcgg caaagatccc aagttccaac gtgcaggcgt
     3541 gaacttcctc tatatcgccc tcttcatcgt ggtcggcggc tcttacgcgg gcaacttctt
     3601 cgcgttgacg cacatccttc ccccggaatt taacttctgg ttcggacacc aaggttacga
     3661 atacctcgat ttgggacgtt tctggcaact cctgctgatg gtcggcctgc tgttgtggct
     3721 gttcctgatg ttgcgttgca cggtttccgc cttcaaagaa aaaggcgtgg acaaaaacct
     3781 gctggcaatc tttgtcgctt ctatggtcgg tgtgggcgta ttctacgcac cgggactgtt
     3841 ctatggcgag aaatccccga ttgccgtaat ggaatactgg cgttggtggg tggttcacct
     3901 gtgggtggaa ggcttctttg aagtatttgc cactgccgcc tttgcctttg tcttctacaa
     3961 tatgggcttt gtccgccgca gtaccgccac tgcctccact ttggccgctg ccgccatctt
     4021 tatgttgggc ggcgttccgg gtacgctgca ccacctgtac ttctccggct ctacctccgc
     4081 ctctatggca atcggcgcct gcttctccgc tttggaagtc gtgccgctgg tgttgctggg
     4141 tcgtgaagca tacgagcatt ggtcttacca acacctgtcc gactgggcga aacgcctgcg
     4201 ttggccgctg atgtgcttcg tggcagtcgc cttttggaat atgatcggtg ccggtgtatt
     4261 cggtttcctg attaacccgc cgatttccct gttctatatc caaggtttga atacttctgc
     4321 tgttcatgcg cacgccgcct tgttcggtgt gtacggtttc ttggcattgg gcttcgtatt
     4381 gctggttgcc cgttatttga aaccgaacgc gcagtttgac gacaaactga tgacttgggg
     4441 cttctggctg ctcaacggcg gcttggtcgg tatgattgcc atcagcctgt tgccggttgg
     4501 cgtgattcag gcatacgcct ccatcacgca cggcctgtgg tatgcacgca gcgaagagtt
     4561 cctgcaaatg gaaatcctcg acaccttgcg ttgggtacgt acggcagctg acttggtctt
     4621 tatcggcggt gcaatctgcg ttgccatcca agccactaaa atcgtattcg gccgcgacaa
     4681 ataagttttg cggtatgaaa tgagcaactg cacccgtttt cgggtgcagt tttttattgc
     4741 ggattgggac gggaatggaa aaggatgtgg attttttatt ttaatcggct atacaattgc
     4801 tgctttcatg ccgtctgaag cacgtttcag atggcatcgg tttacatcag ataaggataa
     4861 aaatgggtat ttacgatttt caaatgaaag atgcggaagg caatgcggtt gatttgtcag
     4921 gctatcgcgg caaggttctg ctgattgtca acacggcaac gcgttgcggt ctgaccccgc
     4981 aatacgaggc tttgcagaag ctgtatgcac aatataccgc agaaggcttg gagattttgg
     5041 attttccgtg caaccagttc cgcgaacagg ctcccgaaag cagcggagaa attgcccaag
     5101 tgtgtatgat gaagtttggc acgaagttta agattttcga caaaatcgaa gtcaatggag
     5161 caaataccgc gcccttgtat gcctacctga aatccgtcaa accgcaagat aaaggcaatc
     5221 atctgtttaa agatttcgtg ttgaagctcg cagctttggg agagaagcgc gacgaaggcg
     5281 acatcaaatg gaactttacc aaattcctcg tcaaccgcga cggcgaagtg gtcgagcgtt
     5341 ttgcgcctag tgttacgccc gaagaaatcg aagccgatat tcgggcattg ctgtaatctg
     5401 ccgccgtctt gggaaatgcc gtctgaaggc ttcagacggc atttgtccgt ttgcgggttt
     5461 attccgaaat atcggcaaca ccgctttcgg ggaagatggg caaatccggc ggtgggagca
     5521 gggtatccaa agtcagggcg gacgcatcga gtgtcgggta gccttcaaac gcgaccgaat
     5581 aaccgctgtt tccgttcata cggattcggg cgtgcgcgcc tagagggaaa gtgattttgt
     5641 cggcaatgtg tccgaacggg aagcccgtca gcacggggat tttcgccgtg cgcgaaacat
     5701 ggttggcaac ggcagaaaaa tcataagacg gatcatagac atctcgaatt ttttccatac
     5761 ggaaattgcc gaacacgatg gcgcgctgtt tcttcaaaat acccgaaaga tacagcgtat
     5821 tgagcatacg ttcgatgcgg tagggctgtt cgccgacatc ttcgaggaac aaaatgccgc
     5881 cgtcgatgtc gggcatataa ggcgtgccgg cgagcgaggc gaggacgctt aagttgccgc
     5941 cccacaatat gccttcggtt tcgacatcgg cgcgttggat ataaggaaca tcaacggtca
     6001 ggcggttttg ggttgcaccc ttgataaagg catccatcgt aaacgcaccg ggggcgggtt
     6061 tgccaaaatc gctataagcc atcgggccgg caaaactcat catattgcct tttgccaaca
     6121 atgccagctg gacggcgcat acgtcgctga atccgaaaaa cagcgtgccg tgttcgcgca
     6181 tccttgcgcc gagcgaagca aaatcgatat gcggcagaat ccgcgccgca ccgtaaccgc
     6241 cgcgcaagcc catcagcact ttaggcgtgg cgacgcggcc ggaagcgacc tcttggaaat
     6301 cggcggcacg ttgtgcgtcc gtaccggcaa accgttggaa acggcggctg cccgcctgtt
     6361 ggttggttac ggtaaaaccg gcattgtaaa ggcgggttaa ggctgtgttg acgcggttgg
     6421 tgtcttcggc aaatcctgac gaagcgacaa tacgcagaag attgtcggca gagttggaat
     6481 ggcgtggtgg tttggggtac acggttcggg ctttcgcaac aggatgagag ggcgcggatg
     6541 gggcattgtt tgaagcaggc gcagtaccgc aagcctgaag cagccccgcg ccggcggcgg
     6601 cggtgcaggt tttcaggaaa cggcggcggg aggtcggttc ggtcataatt tccctttcat
     6661 acggtttcag acggcatcgg cgggcttaaa gcagcttttt aagctgttcc gtccaatctt
     6721 gcggcagggt cataccgtgt ttccggacgg ggtttgccca aatcggcgcg gggaaagacg
     6781 catcgttttc aaagcgggcg ataatatgcc aatgcaggtg cggcacgaca ttgcccaagc
     6841 tggcgaggtt gatttttgcc ggccggaaca cttggcgcat agcggcttcg actttgtaca
     6901 ccatttccat caattcgccg cgttccgctg ccgaaaggtc ggtcatttcg gcgatgtgtt
     6961 catgccagat aacacggcag aatgcaggcg aaccgctgtc gttatggacg gcgatgacgc
     7021 gcagcttggg ggtttgcagc aaaacgtctt cattttgcgc cgtgcagatg gggcagggtt
     7081 gggcggtcat ttttccttat tcctgaaagt gtttaaaaaa cggtaggata atttc
//
LOCUS       DAXHSJ010000111         7089 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000111 DAXHSJ010000000
VERSION     DAXHSJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.111"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            152..781
                     /locus_tag="ACFM8U_001530"
     CDS             152..781
                     /locus_tag="ACFM8U_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC6334750.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILIENNSFDILWTVSGQEKIL"
     gene            937..2613
                     /locus_tag="ACFM8U_001531"
     CDS             937..2613
                     /locus_tag="ACFM8U_001531"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC6334751.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLMLMAFAWLWPGVFSRDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPTAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPTLFAQWLDYHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            2715..3320
                     /locus_tag="ACFM8U_001532"
     CDS             2715..3320
                     /locus_tag="ACFM8U_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC6334752.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LGHYDVKLGKSAAPLLKYAERIFQREAFIDALTPAEKAMRK"
     gene            3389..3781
                     /locus_tag="ACFM8U_001533"
     CDS             3389..3781
                     /locus_tag="ACFM8U_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC6334753.1"
                     /translation="MTTSTKPYILRALCEWCSDNAFTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGKAHDIWIPVGHVLSLFARETGEGMGFELE
                     KYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3810..3959)
                     /locus_tag="ACFM8U_001534"
     CDS             complement(3810..3959)
                     /locus_tag="ACFM8U_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334754.1"
                     /translation="MPSEPHIAGSDGIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(3980..4426)
                     /locus_tag="ACFM8U_001535"
     CDS             complement(3980..4426)
                     /locus_tag="ACFM8U_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC6334755.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTDAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTE
                     VDAAVTETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(4558..4770)
                     /gene="rpsU"
                     /locus_tag="ACFM8U_001536"
     CDS             complement(4558..4770)
                     /gene="rpsU"
                     /locus_tag="ACFM8U_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC6334756.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(5059..6909)
                     /gene="ltgA"
                     /locus_tag="ACFM8U_001537"
     CDS             complement(5059..6909)
                     /gene="ltgA"
                     /locus_tag="ACFM8U_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC6334757.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPADKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQSSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVMGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
BASE COUNT         1400 a         1879 c         1931 g         1879 t
ORIGIN      
        1 agcaccttag agaatcgttc tctttgagct aaggcgaggc aacgccgtac tggtttttgt
       61 taatccacta tattttgatg gggttgaagt tgacatctac aaagtttaat gtttgaatac
      121 attaaaaaaa tatatttgaa tgagatgttt tatgattcaa aatgtcgtta cttcaataat
      181 cctgtattct gggacagccg tagacttact tattatccta atgttatttt ttgccaaaag
      241 aaaaagtaga aaagacatca ttaacatcta tttaggacaa tttctaggct ctgttagcct
      301 gatattgcta agtttgcttt ttgcatttgt cttagattat attcctagta aagagatttt
      361 aggtttgctc ggcttgattc caattttcct aggcatcaaa gttttgctgt taggagattc
      421 tgatggagag gctattgcca aagagggttt gcgcaaagat aataaaaacc tgatttttct
      481 agtcgctatg attacttttg caagttgtgg tgctgacaat attggtgtct ttgtcccata
      541 ttttactacc ttaaatttag caaatttgat agtggcttta cttacctttc tagtcatgat
      601 ttatctcttg gttttttctg cccaaaaatt ggcacaagtc ccttctgttg gagaaacttt
      661 ggaaaaatat agcagatggt ttgttgccgt tgtttattta ggattgggga tatatatcct
      721 gattgaaaac aacagttttg atatactatg gactgtgtcg ggccaggaaa aaatattatg
      781 aaaaagatag tatctgcaaa gactgccatg tgcagtcttt tttgttgccg gtctttttgt
      841 gtctgatgct gtcggatttt atctgatgcc gtctctgcac gacctttgtg cgaatatttg
      901 ctacacttgg caatccgtcc attttttctc acgcttatgc tgacctacac tccgcccgat
      961 gcccgcccgc ccgccaaaac ccacgaaaaa ccgtggctgc tgatgctgat ggcgtttgcc
     1021 tggttgtggc ccggcgtgtt ttcccgtgat ttgtggaatc ctgccgaacc tgccgtctat
     1081 accgccgtcg aagcactggc aggcagcccc acccccttgg ttgcccatct gttcggtcaa
     1141 accgatttcg gcataccgcc cgtgtatctt tgggttgccg ccgcgttcaa acatttgctg
     1201 tcgccgtggg ctgccgactc atacgatgcc gcacgctttg caggcgtgtt tttcgccgtt
     1261 gtcggactga cttcctgcgg ctttgccggt ttcaactttt tgggcagaca ccacgggcgc
     1321 agcgtcgtcc tgattctcat cggctgtatc gggctgattc cagttgccca tttcctcaac
     1381 cccactgccg ccgcctttgc cgccgccgga ctggtgctgc acggttattc tttggctcgc
     1441 cggcgcgtga ttgccgcctc tttcctgctc ggtacgggtt ggacgctgat gtcgttggca
     1501 gcagcttatc cggcagcatt tgcgctgatg ctgcccttgc ccgtgctgat gtttttccgt
     1561 ccgtggcaaa gcaggcgttt gatgttgacg gcagtcgcct cgcttgcctt tgccctgccg
     1621 cttatgaccg tttacccact gctcttggca aaaacgcagc ccacgctgtt cgcgcaatgg
     1681 ctcgactatc acgttttcgg tacattcggc ggcgtgcggc acgttcagac ggcattcagt
     1741 ttgttttact atctgaaaaa cctgctttgg tttgcattgc ccgcgctgcc gctggcggtt
     1801 tggacggtat gccgcacgcg cctgttttcg accgactggg ggattttggg cgtcgtctgg
     1861 atgcttgccg ttttggtgct gcttgccgtc aatccgcagc gttttcagga taacctcgtc
     1921 tggctgcttc cgccgcttgc cctgttcggc gcggcgcaac tggacagcct gaggcgcggc
     1981 gcggcggcgt ttgtcaactg gttcggcatt atggcgttcg gactgtttgc cgtgttcctg
     2041 tggacgggct ttttcgccat gaattacggc tggcccgcca agcttgccga acgcgccgcc
     2101 tatttcagcc cgtattacgt tcccgacatc gatcccattc cgatggcggt tgccgtactg
     2161 ttcacaccct tgtggctgtg ggcgattacc cggaaaaaca tacgcggcag gcaggcggtt
     2221 accaactggg cggcaggcgt taccctgacc tgggctttgc tgatgacgct gttcctgccg
     2281 tggctggacg cggcgaaaag ccatgcgccg gtcgtccgga gtatggaggc atcgctttcc
     2341 ccggaattga aacgggagct ttcagacggc atcgagtgta tcgacatagg cggcggcgac
     2401 ctgcacacgc ggattgtttg gacgcagtac ggcacattgc cgcaccgcgt cggcgatgta
     2461 caatgccgct accgtatcgt ccgcttgccc caaaatgcgg atgcgtcgca aggctggcag
     2521 acggtctggc agggtgcgcg cccgcgcaac aaagacagta agttcgcact gatacggaaa
     2581 atcggggaaa atatattaaa aacaacagat tgagccgaat ttctggatta agtgccggaa
     2641 atcgcgtata attgcgcgat taaaccttta taaatcagcc gttttgcagg catcacacag
     2701 gagcgacaat tattatgatg accctctatt ccggcattac ctgccccttc agccaccgct
     2761 gccgcttcgt tttgtacgaa aaaggtatgg attttgaaat caaagacgtc gatatttaca
     2821 acaaacccga agacctcgcc gtcatgaatc cgtataacca agttcccgtg ctggtcgagc
     2881 gcgatttggt gctgcacgag tccaacatca tcaacgaata catcgacgaa cgcttccctc
     2941 atccgcagct tatgcccggc gatcccgtta tgcgcggccg gggccgactg gtgctgtacc
     3001 gtatggaaaa agaattgttc aaccacgtcc aagtgttaga aaaccccgcc gccaccaaca
     3061 aggaacaggc aaaagcgcgc gaagccatcg gcaacggtct gaccatgctt gccccttcgt
     3121 tcagcaaaag caaatacatc ctcggcgaag atttttctat gattgatgtc gcccttgctc
     3181 cgctgctgtg gcggctcggc cactacgatg tcaaactggg caaaagtgcc gcgccgctgc
     3241 tcaaatacgc cgagcgcatc ttccagcgcg aagcctttat cgacgcgctg acacctgcgg
     3301 aaaaggcgat gcgcaaataa gtttgaaatg cctgtaaaac ctgacggttt caggcatttc
     3361 tatattcagg cacattatgg ggagcattat gactacttcc accaaaccct acattctccg
     3421 cgccctctgc gaatggtgca gcgacaacgc ctttacccct cacatccttg tctgggtcaa
     3481 cgaacacacg cgcgtcccca tgcagtacgt ccgcgacaac gaaattatgc tcaacatcgg
     3541 cgcgaccgcc acgcaaaacc ttcaaatcga caatgattgg atcagttttt ccgcccgttt
     3601 cggcggaaag gcacacgaca tatggatacc tgtcggacac gtcctcagcc ttttcgcacg
     3661 ggagaccggc gaaggtatgg ggtttgaatt ggagaagtac cgacccgata cgccgcctga
     3721 aaacacctct gccgaaaccg cgccccggcc cgccaaaaaa ggcttgaaat tggtcaaata
     3781 aatctatgcc gtctgaacgg aatcgtgttt cagacggcat tttgtccgat ggggcgcaaa
     3841 cggaatccgt ttatcggcaa aacccgtttc ggtgtatcga aaccgtgttg cccctgccga
     3901 ttcgatattg ggacattgaa aatgccgtct gaacctgcga tatggggttc agacggcatt
     3961 tttccttata ttcatacaat caggcggtca gtacggcttt cagaattttg ttgacttcgc
     4021 ccatatcggc tttgccggcg aggcgggttt tcaatacgcc catcactttg cccatatccg
     4081 ccatacctgc cgcgccggtt tcggtaacgg cggcatcgac ctcggtacgg atttcgccgg
     4141 cggaaagcat ttggggaagg tagcggtgca gtacctcgat ttcggcgttt tctttgtctg
     4201 ccaagtcctg ccgccccgcg tcagtataga ttttcgcgct gtctttgcgc tgtttgacca
     4261 ttttggtcag gatggcggtg attttggcat cgtcggcttc ggtgcgttcg tccacttcaa
     4321 actgtttgac ggcggcgttg ataaggcgga tggtgccgag ggaaacttgg tctttggcgc
     4381 gcatagccgt tttcatgtct tcggtaaggc ggattttcag gctcataatg ttctcgctgg
     4441 gatgtcggat ggaaacggcg ggtttcgatg ccgtctgaaa agcaaaacac accgcagagc
     4501 aggtcttgcg gtgtgtcctc atatcacagg gctgacctgt aatctatact tgaatgttta
     4561 gtacattttg ggcggcagtt gttggctgcg caggcgtttt tgcaggcgtt ttacggctgc
     4621 cgcttttttg cgtttgcgtt cggtagtcgg tttttcgtag gcttcgcggg cgcgcagctc
     4681 ggtcagcagg ccggtttttt ctacggcgcg tttgaaacgg cgcatagcga cttcaaatgg
     4741 ttcattctct tttacgcgga ttgcaggcat tttatttcct ttaataaatt cggttgtttc
     4801 atctgcccat catatcggtg gaaagggtag ggcagacggt gttgtaggtt ttccgtatcg
     4861 ctgtgccttt cggcggcggg atacgcgccg tgacggaaac atcacctcct aacgggtcgg
     4921 cttgtgccgt gtcaagctgc ttgaaaaagc aggaaaaaca attttcggat tgtcttatat
     4981 ttatggttcg ccgtcaatgc cgttcgggat aaaaatgccg tctgaaaggc cgggcgagct
     5041 tcagacggca tcggtacgtc agcgggcggg aacaatgccc atacgctgtt tgagcgggat
     5101 gtgcggcgcg ccgaagaggg cggcgtagta ggcggcattg gtcatcactt ttttgacata
     5161 gtcgcgcgtt tcggaaaacg ggatggtttc ggcatatacc gcgccttcga ggggcgtgtc
     5221 cgcctgccat cgacgcgccc tgccggggcc ggcgttgtag cctgcggtgg cgaggacttc
     5281 gttgttttgc aggcggcgtt tggtgtccgc catataccac gtccccatac ggatattgcc
     5341 gtcggcggtg taaagttgtg cggcatccat accgattttg ccggcgattt cgcgcgcggt
     5401 ggcaggcata acctgcatca gcccctgcgc gcctacgcgg gattgcgcgc ccataacgaa
     5461 gcggctttcc tgacgaatca gcccgtaaac ccacgccgga tcgacattaa cattttgcgc
     5521 gtggcggatt accgtgtctt taaacggcga aatgtagcgc aaggtgtagt tgagtttgcg
     5581 gtcggtgcgt tccgcgctgt tgaccgccat atcgtaaaaa ccgtggtcga acgcggtttg
     5641 cgcggcggtc agcagcttgt cttcatcgaa gccgcgtgtg gcgaaacgcc attccgcctg
     5701 agcctgacgg cgcattttcg catcgccggc ggttcggctg tttcggaaca gcaccagcgc
     5761 gcgcttaatc gcgccgtctt ccgccatacg gaggacgctg tttttgccgg catcgggcac
     5821 attgttgcgc gtatcgattt tgcgccccaa ctcttccccc gccagcaccg cataaaaatt
     5881 cctgcccgtc gctgccgcct gtttgtaaag tttttccgcc tcttgcgtgt tgcccgttgc
     5941 ggcgcgactg cgtgccagcc agtagagcca ggtcgggctt ttttgcagtt tttcgggcat
     6001 atgcgagatt acggaagcca gctcgtccca acgtcgggcg cgcaaggcgg cgcgggcgta
     6061 ccactcgatt tggtcgtcgg tcagttggcg gcggtcggca actttgccgt aatagtccaa
     6121 ggcggcaggc acattgaggc tttgcgactg ataatgcccc aatacgcccc acgcgaaact
     6181 gctctgttcg aggcttaaac cgctttccat ttcggacagc agggcggcgg cattcggcga
     6241 tttgcgtgct tctttgccga tgacgttcaa cagggcatat tcgcgcgaac cttgtgtacc
     6301 gccgtcaaac gggctgccca atgcggcggc aaggttgcgt gcgtctgtgg tttggcggcc
     6361 ggccagcagt ccgcgcacgc gcctccaggc gtcgttgccg tccaacaagc cggatgcggc
     6421 tgcctgttcc aacagtttgg tgcagcccga aggcagtttg cccgtatttt tgaccagttc
     6481 ggcggcacgc gtatagtcgt tgcggctcga atcggcgtag cattcgactt cttgggcgcg
     6541 ccctgccggt tcgagtttgg cgtattcctg tgcaaacagc gtccactgtt tgcgtgcgcc
     6601 caaagacttc agccactcgt tgcggacatt ttccgccatc gcactgtcgc cggcgttttc
     6661 caaataggcg gcgacggcgg catcgttttt ctgtttcact gcatccagtg cggacgggta
     6721 gccgccgtaa tctgccaacg tttttccttc gggttcggca gggcgggtgg gaacgcttgc
     6781 cgaaaggtcg gcagtttcta tattgtctgc cggggtcttg tcggctggca gtgtgtttgt
     6841 cgaagaacac gcggcaagca ccagggccgc cagcagcggc agggaatgct tcatagaggg
     6901 taggtacatc ggatttcctt aagaatcgga accctgaacg gtcagggttg gaaaagacaa
     6961 aatgccgtct gaacaggcgt ttgcatgaat tatatgccga aactgcaccg gctttggaat
     7021 gtttccgaca taatttatat ttttcaatca tttgccgttt tggtgcgaac cgctgccttt
     7081 gcccgtttc
//
LOCUS       DAXHSJ010000112         7013 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000112 DAXHSJ010000000
VERSION     DAXHSJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.112"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(76..1503)
                     /locus_tag="ACFM8U_001538"
     CDS             complement(76..1503)
                     /locus_tag="ACFM8U_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC6334758.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPG
                     FEILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFIT
                     IFPVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNM
                     AGGDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGY
                     MMWSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1861..2220)
                     /locus_tag="ACFM8U_001539"
     CDS             complement(1861..2220)
                     /locus_tag="ACFM8U_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC6334759.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLMMFWQFPQRWWVGAVS
                     SIFCSLVAIWMWRRPES"
     gene            complement(2217..2822)
                     /locus_tag="ACFM8U_001540"
     CDS             complement(2217..2822)
                     /locus_tag="ACFM8U_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFC6334760.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFGVFNDMLDIAD
                     TAIIENGLEGIIQIAPFHPDFQFEGTDSDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2869..3351)
                     /locus_tag="ACFM8U_001541"
     CDS             complement(2869..3351)
                     /locus_tag="ACFM8U_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC6334761.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3555..3652
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3863..4963
                     /gene="gcvT"
                     /locus_tag="ACFM8U_001542"
     CDS             3863..4963
                     /gene="gcvT"
                     /locus_tag="ACFM8U_001542"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC6334762.1"
                     /translation="MITLKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVNNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALVA
                     LKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGQGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEVDVRVLKLPFVRNGQKQFD"
     gene            5125..5511
                     /gene="gcvH"
                     /locus_tag="ACFM8U_001543"
     CDS             5125..5511
                     /gene="gcvH"
                     /locus_tag="ACFM8U_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC6334763.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDSYGAGW
                     FFKIKPVNPADYDGLLTAEQYAGEVD"
     gene            5663..6910
                     /gene="hemA"
                     /locus_tag="ACFM8U_001544"
     CDS             5663..6910
                     /gene="hemA"
                     /locus_tag="ACFM8U_001544"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC6334764.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEIRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1782 a         1694 c         1969 g         1568 t
ORIGIN      
        1 tgtcggaagg gaaaaatatt gatttgatta tgccggaatt taaaatgccg tctgaatgtt
       61 cagacggcat agcgtttaca gcagtttgaa aacgaaaaag ataagggtat gtacgatgaa
      121 gacgggtgtc aggaaggcga ccgaccacat catatagccg aagaaagtcg gcatcggtac
      181 gccgcgctgt tcggcaatgg ccttgaccat gaagttcggt gcgttgccga tgtaggtcag
      241 tgcgcccatg aataccgaac ccatagaaac cgccagcagc gaatgaaaca gggtacccgt
      301 catcaaggct tgggcatcgc cgcccgccat attgaaaaaa acgagataag tgggcgcgtt
      361 atccaagaat gccgacaata tgccgctcat ccaaaaatac atcacattaa tcggatgacc
      421 tgccgtatcg tgaaccagcg ataccacccc gcccagcgcg cctgcctcgc ctgctttcag
      481 aatgctcagg acgggaaaga tggtgatgaa gatgccgagg aagagtttgc ccacttcggc
      541 gatgggttca aagttgaatt cgttgcctgc gcggacttgt ttgggcgtga ttgccataga
      601 tacggcggtc aatgcaatca ggatgacatc gcggacgagg ttttgcaggg cgtaacggct
      661 gccgaggatt tcaaatcccg ggtgttcggg tttccaaagg ccggacatta gaaccgcgcc
      721 gaccacgccc gaaagcagga ggaagttcca tttgccgaag atggcgattt tttcgggttt
      781 ttcctgttgt tgttgtgccg gcgtatcttg tgcaatgctt tcctgtttga agaaacggtt
      841 gtcgatgaaa tagaaggcgg tcaacaggac agcggtgctg atcaggacgg gggcgaacat
      901 atgtttgacc gtccacatga aatctacgcc tttgaggaag ccgaggaaga gtggggggtc
      961 gcccaaaggg gtcagaccgc cgccgatgtt tgcaaccagg aaaatgaaga agatgacgat
     1021 gtgcacgcgg cgggtacggt tttggttggc tttcagcagc ggacgaatca tcagcattgc
     1081 tgcgccggtc gttcccatga tagaggcaag tgccgtaccg acggcaagca gggcggtgtt
     1141 gagcttgggt gtgccgttca agtcgcccca aaccaaaatg ccgcctgaaa tggtgtacag
     1201 ggcaagcagc agcaggatga aggggatgta ttcttcaacg agtgcgtgtg cgacggtatg
     1261 gataccggcg gacgcgccaa aaaccaaact gaacgggatg aggaagagca atgtccaaaa
     1321 ggcggtgatt ttgccgtaat ggtgatgcca ggtatgcgaa aaaaacaagg gacccaatgc
     1381 gatagacagc aaaatcaggg caaagggcag gctccacagc aggtttaggt ttgcgccgtc
     1441 caaatctgcg gcgtaaaccg atgcggggaa aagcattagt gaaaacaggg gtaggtggcg
     1501 catcgtgttt cctcgattca agcactgcct tgcgcggcgc gtgggagtga tacaggcacc
     1561 gtgccgcccg gacataggcg gactgtgctg cctgtttgtt tttgaaaaaa gttttccgat
     1621 tgattgtaaa gtaaataatc aggataaacc agtttcccaa accggaaggc ggcgggaagg
     1681 cggattgctg tgcttgggaa tatatgttct ttttgataaa taattttatt taaaacaaat
     1741 aaatatatgt ctttaataaa taatctatcg aaaacgaaaa atgaatttat tttaacatat
     1801 atttgcaatg aaacaggttt gccccccgtt tgtttgccct tatccctttc agtacggcat
     1861 tcaagattcg ggcctgcgcc acatccatat ggcgacaagg gaacaaaaaa tcgatgaaac
     1921 cgccccgacc caccagcgtt ggggaaactg ccaaaacatc atcaggcagg atgcggtcat
     1981 catactgatg gcgaaaatct tggctttgcg cggcactgcg ccgttttgtt cccagttatg
     2041 aaccatcggg ccgaaatagc ggtgccggtg cagccagcgg tgaaagcgcg gggatgcctt
     2101 tgcccagcag gcggcggaga gcagtacgaa cggcgtggtc ggcaacagcg gcagaaaaat
     2161 gccgatgatt cccaacagca gggagatgcc gccgcaggca attaaaagat aacgtatcat
     2221 tttgaaatat ttttcttatt gtgcggataa gggcaggatg tgataccgag ttttgcccag
     2281 ccttcatgtc ccattttttc cagcagggcg atattgcgtt cgaatattgc cgaagcgtcg
     2341 gggaaggctt gtgcggcttt ggcaatgctg tcttcgcgta tcagatgcag tgtcggatag
     2401 ggagaacggt tggtgtagtt gccgatgtcg tccgaatccg tgccttcaaa ttggaaatcg
     2461 ggatgaaacg gggcgatttg gatgatgcct tccaagccgt tttcaataat ggcggtatcg
     2521 gcaatatcga gcatatcgtt gaatacgccg aaatcgggga atagggtcgg gtgaaccagc
     2581 agggtggttt ccagttcggt ggcgggtgta ttgcccagtc gctgcagttc ttcgtccaag
     2641 tcttccaaaa aaccgtcaag gtgtttggct tcgctgatcg cgatgcggac aaggttttta
     2701 acgtgggggg ctttggcaaa gggacacagg ttcagaccga tgacggcttt ttccaaccat
     2761 tgtccggtgt gttcggcaac agcatcttta ttttcggaag tattgatatt cattattgtc
     2821 atgtaaatgt gtttgcagat tgcacgtgcg ggaaaatcgg gaagggcact attccttcag
     2881 caggtggttg agcggcaggg atgtggtgtg tttgatttct tttaaaacaa agctcgattg
     2941 ggcatcctgt acgccgtggt gggacaggag cgtatccaaa acaaaatggg aaaacgcgtt
     3001 catatcggta aaaaacgcct gaagcaggta gtcggtttcc cctgtcaggg cgaagcagct
     3061 caagacttca ggccattttc gaaccgatgc ggcaaagtct tcccgcgcgt cttttgcttt
     3121 gcggatggaa acgcggataa atgcctgaag ccccaggttg acggattccg gagacagcag
     3181 cgcggcatat tggcggacga tgccggcatc ttccaactgc ttcagacggc gcaggcacgg
     3241 agaaggcgaa agtgcgacac gttcggacag ttcgacattg gtcagcctgc cgttttcctg
     3301 gagaacctgt aagattttaa tatcggtttt gtctaaagtg agttggggca tatttgcgtt
     3361 ccgttttaag gaattcggat tgtctgtccg tatgtttgcg gcaatccgca cagatggaga
     3421 ccatattaac atataaaaag ttataccgtc atccgggaca aattttgttt tcggaaaatc
     3481 atgtgaaaac agaggcggtc ggtttgcatc tcttttaaga cggcttgccc aaaccgccga
     3541 ttcaagacat aatcgggaaa tgtgcaggag agtgttacac ccaactacaa tgtaaccacc
     3601 gaaggcgcag acacccttaa atcgctcagg tatcagggac tgcacattga aacaaacaat
     3661 ctggagagcg gcgttggaat aacgttcacc gaaggggaga aggccgtctg aaccaccatt
     3721 cagacaaccg cgcaaagcag tgagcagact ggtttgccat catgcggata cggccgaaaa
     3781 tctcaggttc aaggacagat agggtcatcc gcgcacaggt gcgcgggtgg catctgaaca
     3841 aaaaatccgg agaaacttga gaatgattac tctgaaaacc accccatttc atcaagccca
     3901 tcaagatgca ggcgcgaagc tggtcgattt tgccggctgg gagctgccca tccattatgg
     3961 ttcgcaaatc gccgaacacg aagccgtgcg caccgacgcc ggtatgtttg acgtatccca
     4021 tatgctcgtt accgacgtag caggcgcaaa tgccaaagcc tttttccgca aactgattgc
     4081 caacgacgtt gccaaactcg cttttgtcgg caaagccctt tattccgctt tgctcaacga
     4141 caacggcggc gtgattgacg acttaatcgt ttaccgcacc aacgaagccg aaacccaata
     4201 ccgcatcgtg tccaacggcg cgactcgcga aaaagatacg gcacaattcc acaaagtcgg
     4261 acaagagttc ggcgtttctt tcaatccgcg ctacgacctc ggcatgctcg ccgtacaagg
     4321 cccgaaagcc atcgagaaac tcctgaccgt caaacccgaa tgggcagatg tcgtcaacaa
     4381 cctcaaaccg ttccaaggcg cggatttggg caacgactgg tttgtcgccc gcaccggcta
     4441 taccggcgaa gacggcgtgg aagtcatcct gcccggcact gaagccgtcg cattcttcaa
     4501 agccctgcaa caagccggcg tacagccctg cggcctcggc gcgcgcgata ccctgcgcat
     4561 ggaagccggt atgaacctct acggcaacga tatggacgac gataccagcc cgctcgaagc
     4621 aggcatgggc tggacggttg acttgaaaga cgaaagccgc gacttcgtcg gtaaagccgc
     4681 attggtggca ttgaaagaaa aaggcgttgc cgtcaaacaa gtcggtttgc tgctcgacaa
     4741 aggcggcatc ctgcgcgcgc atatggaagt gttgaccgac aaaggccaag gcgaaaccac
     4801 cagcggcgta ttctcgccca gcctgaagca atccatcgcc atcgcccgcg tgccgaaaga
     4861 ctttgacggc gataccgcca aagttctgat tcgcggcaaa gaagtggacg tacgcgtact
     4921 gaagctgccg tttgtgcgca acggacagaa acagtttgat tgatgcggtt tcagacggca
     4981 ttttcatttc atatgccgtc tgaaagcagg ttttaattgt tgtccgatac ggacgtttgt
     5041 agaaagcatt gaacaaggca tctgtggata ttgattcatg cagatgccgt ctgaaaataa
     5101 cccctatcaa tggagtatca aaccatgagc aacaacatcc cggccgaact gaaatacgtt
     5161 gccagccatg aatggctgcg ccttgaagaa gacggtacga ttaccgtcgg cattacccac
     5221 cacgcgcaag agctgttggg cgacatcgtg ttcgtcgagc tgcccgaagt cggcgcgaac
     5281 cttgccgctg aagagcaagc cggtgtggtt gagtctgtaa aagccgcgtc cgacgtgtac
     5341 gcaccgattg caggcgaagt cgttgccgtc aacgaagatt tgccaagtgc tccggaaact
     5401 gccaacagcg attcttacgg cgcaggctgg ttcttcaaaa tcaaacctgt caatcctgcc
     5461 gattacgacg gtctgctgac tgccgaacaa tacgcgggcg aagtagatta aaccgcccgg
     5521 ctgcccgacg gcaaccgccg gacaaacgga aactgcacct tcagacggca tttttgcggt
     5581 cggaggtgca gttttttgtc cgtgttttaa ggaagcagtt aggctataat aacggtctat
     5641 attcatcttt accgattttt tcatgcaact taccgctgtc ggactcaatc atcaaaccgc
     5701 acctttaagc atacgggaaa agctggcgtt tgccgccgcc gccctgccta aagccgtccg
     5761 caatcttgcc cgaagcaatg cggcaacgga ggcggtaatc ctttctacct gcaaccgcac
     5821 cgagctttac tgcgtcggtg attcggaaga aatcatccga tggcttgccg attaccacag
     5881 tttgccgatt gaagaaatcc gtccgtatct gtacgcgctg gatatgcagg agactgtgcg
     5941 ccatgctttc cgcgtcgcct gcggcttgga ttcgatggtg ttgggcgagc cgcagatttt
     6001 aggacagatt aaggatgcgg tcagggttgc tcaagagcag gaaagtatgg gtaagaaact
     6061 caatgccctg ttccaaaaaa ccttttccgt tgctaaagag atccgtaccg ataccgctgt
     6121 cggcgagaac tcggtttcca tggcttccgc ttccgtcaag ttggcagagc agattttccc
     6181 cgacatcggc gatttgaatg tcttgtttat cggtgcgggt gagatgattg agttggttgc
     6241 tacttatttt gccgccaaaa gtccccggct gatgacggtt gccaaccgga cgctggcgcg
     6301 tgcacaggag ttgtgcgaca agctcggtgt caacgccgaa ccgtgcctgc tgtccgatct
     6361 gcctgccatt ctgcacgatt acgacgtagt ggtttcttca acggcaagcc agcttccgat
     6421 agtcggcaaa ggcatggtgg agcgtgcatt gaaacaaagg cagagtatgc cgttgttcat
     6481 gcttgatttg gcagtgccgc gtgacattga agcggaagtc ggcgatttga atgatgccta
     6541 tctttatact gtggacgata tggtcaatat cgtccaaagc ggcaaggagg caaggcagaa
     6601 ggccgccgcc gccgccgaaa cgctggtgtc cgagaaggtt gccgaatttg tcaggcagca
     6661 gcagggcagg cagagcgtcc ccttgattaa agccttgcgg gacgagggcg agaaagcgcg
     6721 caagcaggtg ttggaaaatg cgatgaaaca gcttgccaaa ggcgcaacgg cagaagaggt
     6781 tttggaacgg ctgtccgtcc aactgaccaa caagctgctg cattcgccga cccaaacttt
     6841 gaataaggcg ggggaagaag ataaggattt ggttcacgcc gtcgcgcaga tttatcattt
     6901 ggacaaataa cggtgcgccg gaaaaggatg cgtctgaagg ggtttcagac ggcatatttg
     6961 tgtgccgcct gacaacatcg tgaaatccca cattatatcg atgtaatcac aaa
//
LOCUS       DAXHSJ010000113         6969 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000113 DAXHSJ010000000
VERSION     DAXHSJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.113"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            409..615
                     /locus_tag="ACFM8U_001545"
                     /pseudo
     CDS             409..615
                     /locus_tag="ACFM8U_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            615..812
                     /locus_tag="ACFM8U_001546"
                     /pseudo
     CDS             615..812
                     /locus_tag="ACFM8U_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(1004..2407)
                     /gene="mtrE"
                     /locus_tag="ACFM8U_001547"
     CDS             complement(1004..2407)
                     /gene="mtrE"
                     /locus_tag="ACFM8U_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC6334765.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREATYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDAAKLRQQAQIVAYEAAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(2462..5665)
                     /gene="mtrD"
                     /locus_tag="ACFM8U_001548"
     CDS             complement(2462..5665)
                     /gene="mtrD"
                     /locus_tag="ACFM8U_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC6334766.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(5677..6915)
                     /gene="mtrC"
                     /locus_tag="ACFM8U_001549"
     CDS             complement(5677..6915)
                     /gene="mtrC"
                     /locus_tag="ACFM8U_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC6334767.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPAVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIASITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1613 a         1956 c         1836 g         1564 t
ORIGIN      
        1 atcaaacatc aaattggact gaccaatcag ggcggattct aatgacacgc cgttcccgcc
       61 gtcaacggca tttacctcgc accgcccccg aaacacagga aaaaactaca ccaaactaca
      121 atttttgttt atgcaaatat ttgttttgac aggatttaaa cgaaagctcc gattcaaatc
      181 tgccgaaccg cccaaaaata tatagtggat taacaaaaat caggacaagg cgacgaagcc
      241 gcagacagta cagatagtac ggtaaggcga ggcaacgccg tactggttta aatttaatcc
      301 actctattga cctgaatatt aaagtttcgc aaagtaatgc aacgttgctt taattggttt
      361 gaccactatt gccgacgatt agaaaaatat tttcagagat gttcaattat ggaaacttgg
      421 gttcaaaact acacggcaat cggcggcagc ctgtatctga ctgccgccgc cgcactctta
      481 cccatcgtct ttttctttgc cgcgctgacc gtcctgaagc tgaaaggcta tcaggcgggg
      541 ctttatacgc tgctgattgc gcttgccgtt gccgtattcg gcttcgggat gccggcgggt
      601 atggcggttt cttccctgcc gccgcagccg cattgaccca acgcccctac gccacgctgg
      661 atttgaccgc gcattacaaa atcggcaaat ccacccgcat cggtttggac tttgaaaacg
      721 tgttcaacaa acgctaccgc cctatgcccg acattcacgt ttacggcacg ccgcgcagcc
      781 tgaccgcaac cgtcaaacat atagtggatt aacaaaaatc aggacaaggc gacgaagccg
      841 cagacagtac aaatagtaca acttggtgct tcagcacctt agagaatcgt tctctttaag
      901 ctaaggcgag ccaacgccgt actggttttt gttaatccac tataaagaaa gaaatgccgt
      961 ctgaaacctt atcgtttcag acggcatttt attgcccgac cggttatttg tcggtttggg
     1021 tatcccgttt caatccgccg ccgagtgcct tgtacaaatc ggcaaggttt tcggcgcggg
     1081 tcagttgtgc cgacaaagcc gcaccttccg ccgaatagct gctgcgttcc gcatcgagca
     1141 aatcaagcgc gccggatacg ccgtgtttgt aacgcagacc gaccaaacgc aacgcttctt
     1201 tagaggcgcg gctttgtttg cttaaagcgt cataggcttt atccagctgc tcgcgcgccg
     1261 ccaatgcgtt tgccacgtct tgaaaggcgg attggacggc ggcttcatag gcaacgattt
     1321 gtgcctgttg gcgcagcttg gctgcatcaa ggttggcttt gttcgtgccc caggtaaaaa
     1381 tcggcagggt aatagacggc gcaaacgacc aaacgcccgt gccgcttttg aacagcccgc
     1441 ccaattcggc agaacccgta ccaacgcttc cggtcaggcg gatggatggg aaaaaggcgg
     1501 cgcgtgccgc accgatattg gcgtttgcct gtttgagcgc gtgttcggca gcacggatat
     1561 cgggacggtc gagcaatact tcggaactca aaccggccgg cagtttttca acaaaaaact
     1621 gcttgtccaa cggcaaaccg gcgggcaggt cgtcgggtat cggttggtta atcaaggttg
     1681 ccaaggcatt gcgcgcctgt tcgcggctgc gcgcggcatg ggcataatcg gctttggcgg
     1741 attcgatgag ggcttcctgc tggcgtaagg cgacggcgga aatcacgcct gccttgtaac
     1801 gcaattcaga cagcttgtag gttgcttcgc gcgttttcaa gacacgctgc gccaaagaca
     1861 tcgcttcttc ggcataacgt tcgttgaaat aggctttggc aacggtggca atcaggctca
     1921 aatgtgccgc atcgcggttg gcggtgctgg caaaatagcc ttgcagtgct gcctcgctgc
     1981 tgctgcggac gcgtccgaac aggtcgagtt cgtaagatgc cgcacccagt ccgactttgt
     2041 agctgctgct tacattgccg ccgctcaagc tgccttggcg cgagtcgttc gcattggcgg
     2101 caagcgtggg caggaggttg ttgcgctcaa tcatgtattg tttgcggtag atttcgctgt
     2161 tcaatacggc ggtacgcaaa ctggtattgc gctcgagtgc gatgtcgatc agcttttgca
     2221 ggcgcgggtc ggcaaaatag tcatgccaac ccaaatcgac cgcacggatg ccgctgtcgg
     2281 cggtatcgtt tttgaacgtt tcggcaactt cgactttggg ctgctcgtat tggggaatca
     2341 tggtgcaggc ggataatgcg aaggctgctg caacagaagt caaggtggtt ttcaatgtag
     2401 tatccataaa aaagtcctga tgccgtctga aaacccgtgg gcgttcagac ggcatggttg
     2461 cttaatgttg gctgtcgtcc gaaccggtga tgcccgcttc ggcggcgtgt tttactgcca
     2521 tttcgtgttc gtgcgcggtt tctttgaaga atttgcgcac caccacatag aaaagcggaa
     2581 caaggaacac ggacaagagc gtgccgatga gcatccccca gaatacggtc gtgccgatgg
     2641 cgcgctggct ggcagaactt gcaccaccgg caatatacag gggaaccacg cccaaaataa
     2701 aggcgaacga ggtcatgata atcggacgga aacgcaggcg ggcggcttcc aaagcggctt
     2761 cgaccgcgct tttcccttgc gcttgaaggt ctttggcaaa ctcgataatc aaaatcgcgt
     2821 ttttcgcact caaacccatc acggtaacga aaccgacttg aaagtagatg tcgttggcga
     2881 acgagggaac gctgcccaac agtccttcaa acaggttgcg cccggttacg cccgcagccg
     2941 caccgatcaa acccaacgga atcacaagga tgaccgccag cggaatcgac cagctttcat
     3001 aaagcgcggc aagtaccaaa aatacggctg caaccgccaa accgtacaaa atcagggttt
     3061 gcgagccgcc ttttgcctct tcgcgcgact gtccgcccca ctccaggctg taaccgccgc
     3121 ccaattcgtc aaccattttt tgaaccgccg ccatagcctg cccggtggaa acgccggttg
     3181 caggcgaagc ggacagcttc atcgaaggat aaccgttgaa gcgtacgctc tgttccgtac
     3241 cgttttccca agaaacagta gcaatggtgg aaagcggtac ggcgacgccg gatttgttcg
     3301 gcacggtcag gttcaaaata tcggcaggct gcatacgggc atcctcgtcg gcctgcacca
     3361 tcacgcgttg cagacggcct tggttcggga agtcgctgac ataagacgaa ctcagcgcgc
     3421 ttgccaatgc ggtgcggatg tcggcaaacg aaatgccttg cgccgccgcg gcggcacggt
     3481 tgatgtcgat tttcaactgc ggcgagtctt ccaaaccgcc ggcacggacg gtgctggggt
     3541 caaacaaacc gctggcacgc attttctgaa tcaactcgtt gcgcttcgcc agcaatgcgg
     3601 tatggccggt attgttgcgg tcttgcaggt tgatgctcag acccgaaccg ttgcccaact
     3661 ccagaatcgg aggcgggacg acggcgatgc caaaaccgtc tttaagcgtc cccatcatca
     3721 tacccgtcag cttgccggca atcgcaacgg catcgctgcc gggcgcggta cgctcgttcc
     3781 aatctttcaa tatggcaaaa cccatcgcca tattctgacc gctgcccgaa aagctgaagc
     3841 cggaaacggt aatgatgttt tctatttcag gaatgctttt cgccagttgg gtaacttgcg
     3901 ccaaagtcgc attggtgcgc tcttgggtcg ctcctgcagg cagttgcacg ctgaccatga
     3961 cgaagccttg gtcttcggtc ggcaggaatg aagtcggcag gcgcataaac aggaacacgc
     4021 ccacaaccgc caagccgata tagacaacca tcatgcggaa agtcttacgc agcactttgg
     4081 caacccggcc ttcgtaaccg tgcgtccaac tgttgaattt cttgttaaac cagccgaaga
     4141 aacctttttt ctcttcgtga tgccctttcg ggattgtctt caacattgtg gcacacaaag
     4201 caggggtaag ggtcagcgca aggaaggcgg agaatgcgat tgatgacgcc atcgtcaggg
     4261 caaactgttt gtaaatattg cccgtcgccc cgctgaacat cgccaacggt acgaacacgg
     4321 aaatcagaac ggcggtaata ccgatgaccg cgcccgaaat ctgacccatc gcttttttgg
     4381 tcgcttcttt gggcggcaag ccttcacccg ccataatgcg ctcgacgttt tcaaccacca
     4441 caatcgcgtc atcgaccacg atgccgatga ccaaaaccat cgcaaacatg gtcagtacgt
     4501 taatcgacat gcccatataa gagatgaagg cgaaaccgcc caacagcgaa atcggtacga
     4561 cgatggtcgg aatcagcgta taacggatgt tttgcaggaa gagatacatt acgacaaaca
     4621 ccagcaccat cgcttcgatt aaagtgtgaa tcactttttc aatcgaaatt tcgacgaatt
     4681 tggaagtatc gtaaggggtt ttccagctca taccctgagg aaagtatttt tccaacgtcg
     4741 ccatgcgttc tttaaccgcc tttgccgtcg ccatcgcatt gccgctgttg gacagcatca
     4801 ccgccatacc ggtggtattt acaccgttca gacgggttga ggaagaatag tcttccatac
     4861 ccagtccgac ccttgccaca tccttcaggt aaacattaga accgtcggta ttggcgcgga
     4921 ggatgacgtt gccgaattct tctgccgtac ccaactgccc ttgcgccgtt acggtagccg
     4981 taaccgtctg tccgcgaacg gcgggaagcg aaccgataga acccgctgaa atctggacgt
     5041 tctgggcgga cagcgcgctg ccgatatcgg caaacgacaa attgtagttt tgcagtttct
     5101 taggatcaac ccaaatccgc atcgcgcgtt gcgcgccgaa caggcgtacc tgccccacgc
     5161 cttcgatacg ctgcaactcg ggaacgatat tacgctgcgc gtagtcgttc atctcttcgg
     5221 ttgactgcac atccgacgaa agcatcacaa tcatcaggaa attggaacgc gccttggata
     5281 cggttacgcc gtattgctgg acggttgccg gcagcgtgct caatacttcg gaaagcttgt
     5341 tctgcacttc cacctgcgcc agattctcgt cggtatcggg cgtaaaggtc aggctcacgc
     5401 tgccgctgcc gctcgaatcg gcggaagtgg acatataatc caaaccttcc acgccgttca
     5461 tattccgctc gatcacggaa agcacgctgt cttccattac ctgcgcggac gcacccggat
     5521 aagtggcgtg caaagtaatg gtcggggcgg cgacagacgg atattgcgaa acaggcaggc
     5581 ttttgatgcc gaaaataccc gccgcaataa tgaaaatcga aataacccac gcaaaaatag
     5641 ggcggtcgat aaaaaattta gccatcgatg ccttccttat tccgcttcag aagcagtttt
     5701 ggcttcagat gccgtctgaa cgccggattg aggcgcggcg gcttggtttt cagacggcgc
     5761 ccattctttg ggcgttacct ttttcgcacc cgttatactg gcgatactga tgccttccac
     5821 aaccaccttg tccccgtcct tcagacccga cgtaacaatc caattcgtac cctgctgttg
     5881 cgcaaccgtt acctcgcggg gttccatacc gccttgggca ttcacaatca tcacggtatc
     5941 tttcgcaccg cgcgttaccg cctgctgcgg cacaacaaat gcgttatcca ccgccacttg
     6001 gtccatcagc acgcgcacat acaggccggg catcaggata ttctgatcgt tcggtacggc
     6061 ggcgcgcagg gtaatctgac cggtcgattc gttgacggcc ggatcggcaa acagcaggcg
     6121 gcctttttca gggtaaactg tgccgtcgtc aaatttgatg ccgaccgcaa tcacaccatc
     6181 cgccgccagc agtttgcctt cggctatctg acggcgcaat ttcatcactt cggatgcaga
     6241 ctgggtaacg ttcacataca tcggattggt ttggcggatg gttgccagca cggtcgtatc
     6301 gcccgcattc aacagcgtac cttcggaaac tttggactga ccgataaagc cggaaatcgg
     6361 cgcggtaatg cgcgaacggt tcagattaat gccggcagat ttgattgccg cctgtgctgc
     6421 tttgacacct gcctcggcag aacgtttcgc cgttaccgca gcatcgtatt cctgccggct
     6481 gacggcttcg gcggcaacca aaggcttgta tcgcgccaaa tccgcatccg ctttggcaag
     6541 cgttgcctga gccgttgcca gttgcgcgcg cgcgctttcc agacctgctt cataagtgga
     6601 actgtcgatc tgatacagcg gctgtccggc acggacataa ctgccttctt ggaacaggcg
     6661 tttttggatg atgccgccga cttgggcgcg gacatcggcg gtacgcagcg attccaaacg
     6721 ccccggcaac tcgacggtca atgcgacggt ttgcggatgg acggttacga caccgacgac
     6781 gggcgcaggg gcttcccgac cagcaggctg cccgccctgc gccgcgtctc cgcctttacc
     6841 gcaagacgac agtaccaatg caacggcggc agccaacgcg gccgcacgca tcgccttaaa
     6901 agcataaaaa gccattattt atcctatctg tctggtttga tgtaaagggt tttgccaatc
     6961 aacaggcat
//
LOCUS       DAXHSJ010000114         6835 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000114 DAXHSJ010000000
VERSION     DAXHSJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.114"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="ACFM8U_001550"
     CDS             complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="ACFM8U_001550"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC6334768.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RTATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGRFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1553..1708
                     /locus_tag="ACFM8U_001551"
     CDS             1553..1708
                     /locus_tag="ACFM8U_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334769.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1705..2136
                     /locus_tag="ACFM8U_001552"
     CDS             1705..2136
                     /locus_tag="ACFM8U_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFC6334770.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2270..6598
                     /gene="app"
                     /locus_tag="ACFM8U_001553"
     CDS             2270..6598
                     /gene="app"
                     /locus_tag="ACFM8U_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC6334771.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGIFPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNVGYRDVDFGAEGRNPDQHRFTYKIVKRNNYKKDETHPYEKDYHN
                     PRLHKFVTEAAPIDMTSDMDGNKYTDRTKYPERVRIGSGWQFWRNDQDKGDQIAGAYH
                     YLTAGNTHNQGGAGGGWSSLSGDVRQAGNYGPIPIAGSSGDSGSPMFIYDAEKQKWLI
                     NGVLRTGNPWAGKENTFQLVRKSFFDEILEKDLRTSFYSPSGNGAYTITDKGDGNGIV
                     KQQTGRPSEVRIGLKDDKLPAEGKDDVYQYQGPNIYLPRLNNGGNLYFGDQKNGTVTL
                     STNINQGAGGLYFEGDFTVSPENNETWQGAGVHISEDSTVTWKVNGVANDRLSKIGKG
                     TLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVSGRGTVQLNADNQFNP
                     DKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKESTVTITGNKDIATTGNN
                     NSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNGNITQTNGK
                     LFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKAENFLIKGGQAVVSRN
                     VAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDEKVIASLTNTD
                     ISGNVSLANHAHLNLTGLATLNGNLTAGGDTHYEVTHNATQNGNLSLVGNAQATFNQA
                     TLNGNTSASGNASFNLSDHAAQNGDLTLSGNAKANVSHSAFNGNVSLADKAVFHFENS
                     RLTGQISGGKDTALHLKDSEWMLPSGTELGNLNLDNATITLNSAYRHDAAGAQTGSAT
                     DAPRRRSRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMSELFGYRSDKLKL
                     AESSEGTYTLAVNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEHVDAGAWRYQL
                     IRKDGEFRLHNPVKEQELSDKLGKAEAKKQAGKDNAQSLDALIAAGRDAVEKTESVAE
                     PARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRARRARRDLPQP
                     QPQPQPQPQRDLISRYTNSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVWTSGIRDT
                     KHYRSQDFRAYSQQTNLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNSARLAHGA
                     VFGQYGIGRFDIGISAGAGFSSGSLSDGIGSKIRRRVLHYGIQARYRAGFGGFGIEPH
                     IGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPYLSLSYTD
                     AASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLEAQHSAGI
                     KLGYRW"
BASE COUNT         1804 a         1996 c         1698 g         1337 t
ORIGIN      
        1 caaacggatc ggattcccgt ttttatggga taacggaatg ttcagccgga caccgccacg
       61 atgctttcga tccgtttatt ttccgatgca gaatctcgaa aaaatcacgc cgagcaggtc
      121 gtccgccgta aactcgcccg tgatttcgcc gcatgcgacc tgcgccagcc gcaagtgttc
      181 ggcaaacagc tcgatttgat ggttgccgca caatgccgcc agcgacaatt cttcctgcgc
      241 tgctttgagt gcgttgacgt gccgcgtccg cgccaaaaac agcccttcgc tttcgccctg
      301 ccaaccggcc tcgcgcaaca acgtccgttt cagcgcgtcc aagccgtcgc cggttttcgc
      361 cgacagcgcg atgacggttt ctgcgcccgt accgaacctg cctgccgcgt gtgcgtgcaa
      421 atcggatttg ctgtggattt cgatgcgttt caactccggc ggcaacgcgt ccaaaatcgc
      481 ccgtgtcttt tcattcaaac cctcgcgcgg atcgaccaac accagcgcga catcggcttc
      541 ggatacggct ttgcggctgc gttcgatgcc gatgcgttcg accacgtcgt ccgtctcgcg
      601 caaacctgcc gtatcgacaa tatgcactgg aacgccgtca atcaggatac gttccctgac
      661 cgcgtcgcgc gtcgttccgg caatatcggt aacaatcgcc acttcgtcgc ccgccagcgc
      721 gttcaacagg ctggatttgc cgacattcgg cgcgccgacc aatacgacat tcagaccttc
      781 gcgcaaaatc gcgccctgct gcgcgttggc aagcacatca tccacggcgc ggcgcaagcc
      841 gtccaatttg ccgcgcgcat ccgctgcttc gagaaaatca atgtcttcct cgggaaaatc
      901 cagcgtcgct tcgaccagca tccgcaaggt aatcaaatct tcgaccagac cgtgtatccg
      961 ccgcgaaaaa tcgcccttga gcgagcgcaa cgccagacgc gccgccgaac ggctggatgc
     1021 gtcaatcaaa tccgccacgc cctccgcctg tgccaagtcc agtttgtcgt ttaaaaacgc
     1081 acgcttcgta aactcgcccg gctcggcaag gcgcgcgccc agttccaaac agcggttcag
     1141 cagcatatcc atcaccaccg gcccgccgtg tccctgaagc tcgatgacat cttcgcccgt
     1201 aaaacttgcc ggtgcggcaa aaaacagcaa aagcccgcta tcgattgcct gtccgtccgc
     1261 gtccgtaaaa tcagcatagg ttgcggtacg cggtttaggc gttttcccgc acaaagcttg
     1321 cgccatcggc agcaggtttt tccccgatat gcgtatcacg cccacgccgc cgcgtccggg
     1381 cgcggtagcg actgccgcaa tcgttggaac gttatccgac ataaaacccc cgaaaattca
     1441 aaacagccgc gattatagca aatgccgtct gaagtccgac ggtttggctt tcagacggca
     1501 taaaaccgca aaaatgcttg ataaatccgt ccgcctgacc taatataacc atatggaaaa
     1561 acgaaacaca tacgccttcc tgctcggtat aggttcgctg ttgggcctgt tccatcccgc
     1621 aaaaaccgcc atccgcccca atcccgccga cgatctcaaa aacatcggcg gcgattttca
     1681 acgcgccata gagaaagcgc gcaaatgacc gaaaacgcac aggacaaggc gcggcaggct
     1741 gtcgaaaccg tcgtcaaatc cccggagctt gtcgagcaaa tcctgtccga cgagtacgtg
     1801 caaataatga tagctcggcg tttccattcg ggaccgttgc cgccgccgtc cgacttggcg
     1861 caatacaacg acattatcag caacggggca gaccgcatta tggcaatggc ggaaaaagaa
     1921 caagccgtcc ggcacgaaac catacggcaa gaccaaacct tcaacaggcg cgggcaactg
     1981 tacggcttca tcagcgtcat cctgatactg ctttttgccg tcttcctcgt atggagcggc
     2041 taccccgcaa ccgccgcctc ccttgccggc ggcacagtgg ttgccttggc gggtgctttc
     2101 gtgattggaa gaagccgaga ccaaggcaaa aattaatgac aaatcctagg gcgtgcttca
     2161 tatccgcccg aacgccgaac cgcacatata ggcacatccc gcgcgccgcc cggaagcgga
     2221 agccgcgccc tcccaaacaa acccgaatcc cgtcagataa ggaaaaataa tgaaaacaac
     2281 cgacaaacgg acaaccgaaa cacaccgcaa agccccgaaa accggccgca tccgcttctc
     2341 gcctgcttac ttagccatat gcctgtcgtt cggcattttt ccccaagcct gggcgggaca
     2401 cacttatttc ggcatcaact accaatacta tcgcgacttt gccgaaaata aaggcaagtt
     2461 tgcagtcggg gcgaaagata ttgaggttta caacaaaaaa ggggagttgg tcggcaaatc
     2521 aatgacaaaa gccccgatga ttgatttttc tgtggtatcg cgtaacggcg tggcggcatt
     2581 ggtgggcgat caatatattg tgagcgtggc acataatgta ggttatcgcg atgttgattt
     2641 tggtgcagag gggcgaaatc ccgatcaaca tcgttttact tataaaattg tgaaacggaa
     2701 taattataaa aaggatgaaa cgcatcctta tgagaaagac taccacaacc cccgtttgca
     2761 taaatttgtt acggaagccg ccccgatcga tatgacttct gatatggacg gcaacaaata
     2821 tacagatagg acgaaatatc ccgaacgcgt ccgtatcggc tccgggtggc agttttggcg
     2881 aaacgatcaa gacaaaggag accaaattgc cggcgcatat cattacctga cagcaggcaa
     2941 tacacacaat caaggcggag cagggggcgg ctggtcaagc ctgagcggcg atgtgcgcca
     3001 ggcaggcaat tacggtccca ttcctattgc gggctcaagc ggcgacagcg gttcgcccat
     3061 gtttatttat gatgcggaaa aacaaaaatg gttgattaac ggcgttttga ggacaggtaa
     3121 cccttgggcg gggaaagaaa atacattcca actggtacgc aagtcttttt ttgatgaaat
     3181 ccttgaaaaa gatttacgta catcgtttta tagcccatcg ggcaatggtg catacaccat
     3241 tacggacaaa ggcgacggca acggcattgt caaacaacaa acaggaagac catctgaagt
     3301 ccgaatcggc ttgaaagacg acaaattgcc tgccgaaggt aaagacgatg tttaccaata
     3361 ccaaggtcca aatatatacc tgcctcgttt gaataacggt ggaaacctgt atttcggaga
     3421 ccaaaaaaac ggcactgtta ccttgtccac caacatcaac caaggcgcgg gcggtttgta
     3481 ttttgaaggt gactttacgg tctcgcctga aaataacgaa acgtggcaag gcgcgggcgt
     3541 tcatatcagt gaagacagta ccgttacttg gaaagtcaat ggcgtggcaa acgaccgcct
     3601 gtccaaaatc ggcaaaggca cgctgcacgt tcaagccaaa ggggaaaacc aaggctcgat
     3661 cagcgtgggc gacggtaaag ttattttaga tcaacaagca gatgaaaata ataaaaaaca
     3721 agcctttagt gaaatcggct tggtcagcgg caggggtacg gtgcaactga atgccgataa
     3781 tcagttcaac cccgacaaac tctatttcgg ctttcgcggc ggacgtttgg atttgaacgg
     3841 gcattcgctt tcgttccacc gtattcaaaa taccgatgaa ggggcgatga ttgtcaacca
     3901 caatcaagac aaagaatcca ccgttaccat tacaggcaat aaagatattg ctacaaccgg
     3961 caataacaac agcttggata gcaaaaaaga aattgcctac aacggttggt ttggcgagaa
     4021 agatacgacc aaaacgaacg ggcggctcaa ccttgtttac cagcccgccg cagaagaccg
     4081 caccctgctg ctttccggcg gaacaaattt aaacggcaac atcacccaaa caaacggcaa
     4141 actgtttttc agcggcagac cgacaccgca cgcctacaat catttaaacg accattggtc
     4201 gcaaaaagag ggcattcctc gcggggaaat cgtgtgggac aacgactgga tcaaccgcac
     4261 atttaaagcg gaaaatttcc tgattaaagg cggacaggcg gtggtttccc gcaatgttgc
     4321 caaagtagaa ggcgattggc atttgagcaa tcacgcccaa gcagttttcg gtgtcgcacc
     4381 gcatcaaagc cacacaatct gtacacgttc ggactggacg ggtctgacaa attgtgtcga
     4441 aaaaaccatt accgacgaaa aagtgattgc ttcattgacc aataccgaca tcagcggcaa
     4501 tgtcagcctt gccaatcacg ctcatttaaa tctcacaggg cttgccacac tcaacggcaa
     4561 tctgaccgca ggcggagaca cgcactatga ggttacgcac aacgccaccc aaaacggcaa
     4621 ccttagcctc gtgggcaatg cccaagcaac atttaatcaa gccacattaa acggcaacac
     4681 atcggcttcg ggcaatgctt catttaatct aagcgaccac gccgcacaaa acggcgatct
     4741 gacgctttcc ggcaacgcta aggcaaacgt aagccattcc gcattcaacg gcaatgtctc
     4801 cctagccgat aaggcagtat tccattttga aaacagccgc cttaccggac aaatcagcgg
     4861 cggcaaggat acggcattac acttaaaaga cagcgaatgg atgctgccgt caggcacgga
     4921 attaggcaat ttaaaccttg acaacgccac cattacactc aattccgcct atcgccacga
     4981 tgcggcaggg gcgcaaaccg gcagtgcgac agatgcgccg cgccgccgtt cgcgccgttc
     5041 gcgccgttcc ctattatccg ttacaccgcc aacttcggca gaatcccgtt tcaacacgct
     5101 gacggtaaac ggcaaattga acggtcaggg aacattccgc tttatgtcgg aactcttcgg
     5161 ctaccgcagc gacaaattga agctggcgga aagttccgaa ggcacttaca ccttggcggt
     5221 caacaatacc ggcaacgaac ccgtaagcct cgatcaattg acggtagtgg aagggaaaga
     5281 caacaaaccg ctgtccgaaa accttaattt caccctgcaa aacgaacacg tcgatgccgg
     5341 cgcgtggcgt taccaactca tccgcaaaga cggcgagttc cgcctgcata atccggtcaa
     5401 agaacaagag ctttccgaca aacttggcaa ggcagaagcc aaaaaacagg cgggaaaaga
     5461 caacgcgcaa agccttgacg cgctgattgc ggccgggcgc gatgccgtcg aaaagacaga
     5521 aagcgttgcc gaaccggccc ggcaggcagg cggggaaaat gtcggcatta tgcaggcgga
     5581 ggaagagaaa aaacgggtgc aggcggataa agacagcgcc ttggcgaaac agcgcgaagc
     5641 ggaaacccgg ccggctacca ccgccttccc ccgcgcccgc cgcgcccgcc gggatttgcc
     5701 gcaaccccaa ccgcaacccc aaccccaacc gcagcgcgac ctgatcagcc gttataccaa
     5761 tagcggtttg agtgaatttt ctgccacgct caacagcgtt ttcgccgtac aggacgaatt
     5821 ggaccgcgtg tttgccgaag accgccgcaa cgccgtttgg acaagcggca tccgggacac
     5881 caaacactac cgttcgcaag atttccgcgc ctacagccaa caaaccaacc tgcgccaaat
     5941 cggtatgcag aaaaacctcg gcagcgggcg cgtcggcatc ctgttttcgc acaaccggac
     6001 cgaaaacacc ttcgacgacg gcatcggcaa ctcggcacgg cttgcccacg gcgccgtttt
     6061 cgggcaatac ggcatcggca ggttcgacat cggcatcagc gcgggcgcgg gttttagcag
     6121 cggcagcctt tcagacggca tcggaagcaa aatccgccgc cgcgtgctgc attacggcat
     6181 tcaggcacga taccgcgccg gtttcggcgg attcggcatc gaaccgcaca tcggcgcaac
     6241 gcgctatttc gtccaaaaag cggattaccg ctacgaaaac gtcaatatcg ccacccccgg
     6301 ccttgcgttc aaccgctacc gcgcgggcat taaggcagat tattcattca aaccggcgca
     6361 acacatttcc atcacgcctt atttgagcct gtcctatacc gatgccgctt cgggcaaagt
     6421 ccgaacgcgc gtcaataccg ccgtattggc tcaggatttc ggcaaaaccc gcagtgcgga
     6481 atggggcgta aacgccgaaa tcaaaggttt cacgctgtcc ctccacgctg ccgccgccaa
     6541 aggcccgcaa ctggaagcgc aacacagcgc gggcatcaaa ttaggctacc gctggtaaca
     6601 ccggatatgc cgaaaggggt ttgacgatgc caccgtgcgc tgtcaaaccc tttttctgcc
     6661 gccgccttgt gcctgctcca ttgtctgata accgtcaaat tgatttttag cccatgtttg
     6721 gtgggtcggg gaaatctata tcttcgtccg tgccgaaata gtctgagacc tttgcaaaat
     6781 tcctttccct cccgacagcc gaaaccccaa cacaggtttt cagctgtttt cagct
//
LOCUS       DAXHSJ010000115         6770 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000115 DAXHSJ010000000
VERSION     DAXHSJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.115"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="ACFM8U_001554"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="ACFM8U_001554"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC6334772.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1101..1511
                     /locus_tag="ACFM8U_001555"
     CDS             1101..1511
                     /locus_tag="ACFM8U_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding protein YggL"
                     /protein_id="HFC6334773.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQALIFGVRGSLHQAA
                     DDAEAFDRFMDELIAYLEANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            1532..3595
                     /gene="glyS"
                     /locus_tag="ACFM8U_001556"
     CDS             1532..3595
                     /gene="glyS"
                     /locus_tag="ACFM8U_001556"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC6334774.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMADDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3612..4700
                     /locus_tag="ACFM8U_001557"
     CDS             3612..4700
                     /locus_tag="ACFM8U_001557"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6334775.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGKMRRLFTM
                     RQYFSILHRLIKNRRQARSDSAGKEQEI"
     gene            4700..5527
                     /locus_tag="ACFM8U_001558"
     CDS             4700..5527
                     /locus_tag="ACFM8U_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6334776.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLKERFDPDFAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5539..5634
                     /locus_tag="ACFM8U_001559"
     CDS             5539..5634
                     /locus_tag="ACFM8U_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HFC6334777.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            5676..6518
                     /locus_tag="ACFM8U_001560"
     CDS             5676..6518
                     /locus_tag="ACFM8U_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6334778.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEELNRV
                     MAELVPGLAKQHLLSEVEKACFMSHVVLWKQALDEGVPYVAVFEDDVLLGEGAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVSPALCTQELHYAKFLSQN
                     SMLGSDLEKDREQGRRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKVYGRRV
                     ISFK"
BASE COUNT         1724 a         1803 c         1787 g         1456 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacatt ctgggcagac aaaggctgca
      181 ccgttatcca acccttcgac atggaagtcg gtgcaggtac ttcccacccc gccacctgcc
      241 tgcgcgcact cgggcccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccca
      301 aagacggtcg ctacggcgac aaccccaacc gcctgcaaca ctattaccaa ttccaagtcg
      361 ccctcaagcc cgctcccgcc aatatccaag acctctatct cgactccctg cgcgaattgg
      421 gcatcgaccc caaagtccac gacatccgct ttgtcgaaga cgactgggaa aaccccaccc
      481 tcggtgcatg gggtttgggc tgggaagtct ggctcaacgg catggaagta acccagttta
      541 cctatttcca acaagtcggc ggcattgact gcacgcctgt actcggcgaa attacctacg
      601 gcatcgaacg cttggcgatg tacctgcaag gcgtggaaaa cgtctatgac ctcgtttggg
      661 caaaaacgcc ggacggcaac accgtaagct acggcgacgt gtaccaccaa aacgaagtcg
      721 aacaatccac ctacaacttc gaatacagcg atgccgattg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgcttg ccgaagaaaa cgccgccctc gccctgcctg
      841 cctacgagct ggtcctcaaa gcggggcata cgttcaacct cttggacgcg cgcggcgcga
      901 tttccgtaac cgaacgcgcc acctacatcg ggcgcatccg cgcgttgagc cgcgccgtgg
      961 cgcaaaaata tgtcgaaagc cgcgaaaaac tgggctttcc cttaatgaag gcaaatgccg
     1021 cctaggggag cgacgatttt tacgttcggg cggcacgata tggcagccgt ccggaagcca
     1081 ttatattttt taggaaaatt atgcctgtct atttccgcac cccgagcagg aaacgcctgc
     1141 acaaaatgaa ccgccgccag cataaaaagc tgcatttggg cgagtttcaa gcattgattt
     1201 tcggcgttcg aggcagtttg caccaagctg cggacgatgc tgaagcgttc gaccgcttta
     1261 tggacgagct gattgcgtat cttgaggcaa atggcttgga aatggcgggc gggggcgatt
     1321 gcggcgattt ttcgtgcctg tttcaaaaat ccggtgcccg aagcttgggc gaaaccgaga
     1381 aaaaagccat tatctcttgg ctggcaggcc gcgaagatgt cgccactttg tacgccaccc
     1441 cgctggttga tggtttttac ggggatgacg gctgggaaaa ttacagcgaa acctataaac
     1501 aggcagatta accaaattca aagaaagccg aatgatgacc caaacccttt taatcgaact
     1561 ccttaccgaa gaactcccgc ccaaagccct gaataatctg ggcaaccatt ttgccgccgc
     1621 cgttgccgaa ggcttggaaa aagcgcaact ggttgacggt gcagccgaat ttacggctta
     1681 tgcctcgccg cgccgtttgg cggttcaagt caaaaacgtc aaagccgttc aggccgacca
     1741 aaaaatcgtg aaaaaaggcc ctgccgtggc gaatgccgta aaagacggtg cgccgaccaa
     1801 ggctttggaa ggtttcgcac gcggtgcagg cgcgaaaatc gaagacttga ccatcgtcca
     1861 cgacggcaag caggatgtgt acgcctacga atacgtccaa accggcaagc cgctgggcgg
     1921 acttttggaa gacattatca atcaagtggt taagaaactg ccgattccca aagtgatgcg
     1981 ttggggcagc agcacattta ccttcgtgcg ccctgttcac gggctgattg tgctgcacgg
     2041 cggcgacatc gtaaacgtca gcgttttggg tctgcaaagc agcaatcaaa ccttgggaca
     2101 ccgcttcttg tccaacggcg aaatcaccat tgaaaacgcc gacagctacg ccgaacaaat
     2161 gcgcgggcaa ggcaaagtcg tcgcttcgtt tgccgagcgc aaagccgcga ttcagacggc
     2221 attggaaggg caggcgcgca gtctgaacgc cgccgtcgcc gccgatgaag cattgttgga
     2281 cgaagtaacc gcgctggtcg aatggcctgt tgttttggaa gccggttttg aggaacattt
     2341 cctcgccgtg ccgcaggaat gcttgatttt aacgatgcag caaaaccaaa aatactttcc
     2401 gctgctcgac caaaacggca agctgatgaa ccgcttcctg ctggtttcca acctgcaaac
     2461 cgaagacccg tcgcacatca tccgaggcaa cgaacgcgtt ttgcgcgcgc gcctgtctga
     2521 tgccgagttc ttctacaaac aagaccaaaa agcgactttg gaaagccgcc tgccgaaatt
     2581 gtccagcgtg gtgtaccaca acaaaatcgg ctcgcaagcc gaacgcatcg aacgcctgca
     2641 aagcattgcc gcccacatcg ccaaagcatt gggcgcggat gccgccgctg ccgaacgcgc
     2701 cgcgcgtctt gccaaagccg acttggtaac cgaaatggtc ggcgagtttc ccgaactgca
     2761 aggcacgatg ggcaaatact atgcccgctt ggacggcgaa accgaagaaa tcgccgaagc
     2821 cgtcgagcaa cattaccaac cgcgttttgc cggcgacaag ctgcccgaaa gcaaaattgc
     2881 cactgccgtt gcacttgccg acaagctgga aaccctggtc ggcatttggg gcatcggtct
     2941 gattccgacc ggcgacaaag acccctacgc cctgcgccgc gctgccttgg gtattttgcg
     3001 tatgctgatg cagtatggtt tggacgtgaa cgaactgatt cagacggcat tcgacagctt
     3061 ccccaaaggt ttgctcaacg aaaaaacgcc gtctgaaacc gccgacttta tgcaggcgcg
     3121 tttggccgtg ttgctgcaaa acgattatcc gcaagacatc gttgccgccg tattggcgaa
     3181 acagccgcgc cgtttggacg atttgaccgc caaactgcaa gccgttgccg cgttcaaaca
     3241 gctgcccgaa gccgccgcgc tcgccgccgc caacaaacgc gtgcaaaatc tgctgaaaaa
     3301 agccgatgcc gagttgggcg cggttaacga aagcctgctg caacaggacg aagaaaaagc
     3361 cctctttgcc gccgcgcaag gtttgcagcc gaaaatcgcc gccgccgttg ccgaaggcaa
     3421 tttccaaacc gccttgtccg aactggcttc cgtcaagccg caagtcgatg ccttctttga
     3481 cggcgtgatg gtgatggcgg acgatgccgc cgtgaaacaa aaccgtttga atctgttgaa
     3541 ccgcttggca gaacaaatga acgcagttgc cgacatcgcg cttttgggcg agtaaccgtt
     3601 gtacagtcca aatgccgtct gaagccttca gacggcatcg tgcctatcgg gagaataaat
     3661 tgcagccttt agtcagcgta ttgatttgcg cctacaacgt agaaaaatat ttcgcccaat
     3721 cattagccgc cgtcgtgaat cagacttggc gcaacttgga gattttgatt gtcgatgacg
     3781 gctcgacaga cggtacgctt gccattgcca aggattttca aaagcgggac agccgtatca
     3841 aaatccttgc acaagctcaa aattccggcc tgattccctc tttaaacatc gggctggacg
     3901 aattggcaaa gtcaggaatg ggggaatata ttgcacgcac cgatgccgac gatattgccg
     3961 cccccgactg gattgagaaa atcgtgggcg agatggaaaa agaccgcagc atcatcgcga
     4021 tgggcgcgtg gctggaagtt ttgtcggaag aaaaggacgg caaccggctg gcgcggcatc
     4081 acaggcacgg caaaatttgg aaaaagccga cccgacacga agatattgcc gactttttcc
     4141 ctttcggcaa ccccatacac aacaacacga tgattatgag gcgcagcgtc attgacggcg
     4201 gtttgcgtta caacaccgag cgggattggg cggaagatta ccaattttgg tacgatgtca
     4261 gcaaattggg caggctggct tattatcccg aagccttggt caaataccgc cttcacgcca
     4321 atcaggtttc atccaaatac agcatccgcc aacacgaaat cgcgcaaggc atccaaaaaa
     4381 ccgccagaaa cgattttttg cagtctatgg gttttaaaac ccggttcgac agccttgaat
     4441 accgccaaat aaaagcagta gcgtatgaat tgctggagaa acatttgccg gaagaagatt
     4501 ttgaacgcgc ccgccggttt ttgtaccaat gcttcaaacg gacggacacg ctgcccgccg
     4561 gcgcgtggct ggatttcgcg gcagacggca agatgaggcg gctgtttacc atgaggcaat
     4621 acttcagcat tttgcaccgg ctgattaaaa accgccggca ggcgcggtcg gattcggcag
     4681 ggaaagaaca ggagatttaa tgcaaaacca cgttatcagc ttagcttccg ccgcagaacg
     4741 cagggcgcac attgccgcaa ccttcggcgc gcgcggcatc ccgtttcagt ttttcgacgc
     4801 actgatgccg tctgaaaggc tggaacaggc aatggcggaa ctcgtccccg gcttgtcggc
     4861 gcacccctat ttgagcggag tggaaaaagc ctgctttatg agccacgccg tattgtggaa
     4921 acaggcattg gacgaaggtc tgccgtatat cgccgtattt gaggacgacg ttttactcgg
     4981 cgaaggcgcg gagaaattcc ttgccgaaga cgcttggcta aaagaacgct ttgaccccga
     5041 tttcgccttt atcgtccgct tggaaacgat gtttatgcac gtcctgacct cgccctccgg
     5101 tgtggcggac tactgcgggc gtgcctttcc gctgttggaa agcgaacact gggggacggc
     5161 gggctatatc atttcccgaa aagcgatgcg gtttttcctg gacaggtttg ccgccctgcc
     5221 gcccgaaggg ctgcaccccg tcgatctgat gatgttcagc gattttttcg acagggaagg
     5281 agtgccggtt tgccagctca atcccgcctt gtgcgcccaa gagctgcatt atgccaagtt
     5341 tcacgaccaa aacagcgcat tgggcagcct gatcgaacac gaccgcctcc tgaaccgcaa
     5401 acagcaatgg cgcgattccc ccgccaacac attcaaacgc cgcctgatcc gcgccttgac
     5461 caaaatcagc agggaaaggg aaaaacgccg gcaaaggcgc gaacagttca ttgtgccttt
     5521 ccaataaaag gaggagagat ggacatcgta tttgcagcag acggcaggat gcggcggctg
     5581 tttaccttga ggcaatactt cggcattttg caccgattgc tgaaaaaccg ttgaaaaacg
     5641 ccgctttatc caacagacaa aaaacaggat aaattatgca aaaccacgtt atcagcttgg
     5701 cttccgctgc agaacgcagg gcgcacattg ccgatacctt cggcaggcac gacattccgt
     5761 ttcagttttt cgacgcactg atgccgtctg aagaactgaa tcgggtgatg gcggaattgg
     5821 tgccgggatt ggcaaaacag cacctgttga gcgaagtgga aaaagcctgc tttatgagcc
     5881 acgtcgtatt gtggaagcag gcgttggacg aaggcgtacc gtatgttgcc gtatttgagg
     5941 atgatgtctt actcggcgaa ggggcggaga aattccttgc cgaagatact tggttggaag
     6001 agcgttttga taaggattct gcctttattg tccgtttgga aacgatgttt gcgaaagtta
     6061 ttgtcagacc ggataaagtc ctgaattatg aaaaccggtc atttcctttg ctggagagcg
     6121 aacattgggg gacggctggc tatatcattt cgcgtgaggc gatgcggttt ttcttggaaa
     6181 ggtttgccgt tttgccggca gagtggatta aagcggtaga ttggatgatg tttacttatt
     6241 tctttgataa ggaggggatg cctgtttatc aggttagtcc agccttatgt acccaagaat
     6301 tgcattatgc caagttcctc agtcaaaaca gtatgttggg tagcgatttg gaaaaagata
     6361 gggaacaagg aagaagacac cgccgttcgt tgaaggtgat gtttgacttg aaacgtgctt
     6421 tgggtaaatt cggtagggaa aagaagaaaa gaatggagcg tcaaaggcag gcggagcttg
     6481 agaaagttta cggcaggcgg gtcatatcgt tcaaatagtt tgtgtaaaat atagcggatt
     6541 aaaattagaa atggacacac tgtcattcct gcgagaacag aaaaccaaaa acagaaacct
     6601 aaaatctcgt cattcccgcg caggcgggaa tctagtccgt tcggtttcgc ttgttttaaa
     6661 tttcgggtaa cttccacttc gtcattcccg cgaaagtggg aatccagtcc gttgagtttc
     6721 agtcatttcc gataaatcac cgcaacgtta aatttctaga ttcccgcttt
//
LOCUS       DAXHSJ010000116         6753 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000116 DAXHSJ010000000
VERSION     DAXHSJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.116"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="ACFM8U_001561"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="ACFM8U_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC6334779.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGTRENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(484..681)
                     /gene="rpmI"
                     /locus_tag="ACFM8U_001562"
     CDS             complement(484..681)
                     /gene="rpmI"
                     /locus_tag="ACFM8U_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC6334780.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(828..1349)
                     /gene="infC"
                     /locus_tag="ACFM8U_001563"
     CDS             complement(828..1349)
                     /gene="infC"
                     /locus_tag="ACFM8U_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC6334781.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1367..3280)
                     /gene="thrS"
                     /locus_tag="ACFM8U_001564"
     CDS             complement(1367..3280)
                     /gene="thrS"
                     /locus_tag="ACFM8U_001564"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC6334782.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKATVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VDEARAFNELLVRIYKQFGFHDVAVKLSLRPEQRAGSDEVWEKAEQGLRDALTACGVE
                     WEELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RVELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3334..3410)
                     /locus_tag="ACFM8U_001565"
     tRNA            complement(3334..3410)
                     /locus_tag="ACFM8U_001565"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3374..3376),aa:Val,seq:gac)
     gene            3589..4704
                     /gene="tgt"
                     /locus_tag="ACFM8U_001566"
     CDS             3589..4704
                     /gene="tgt"
                     /locus_tag="ACFM8U_001566"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC6334783.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVIEQFGGLHELIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFQSPINGDKLFLSPEVSMKIQTVLNSDIVMQLDECTPGEATHSQAQKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAIGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRSYLHHLHRVGEILGAQLNTIH
                     NLHFYQVIMAEMREAIEQGKFADWQARFHENRARGVD"
     gene            4714..5796
                     /gene="hemH"
                     /locus_tag="ACFM8U_001567"
     CDS             4714..5796
                     /gene="hemH"
                     /locus_tag="ACFM8U_001567"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC6334784.1"
                     /translation="MPSKAASDGILSLYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            5988..6389
                     /locus_tag="ACFM8U_001568"
     CDS             5988..6389
                     /locus_tag="ACFM8U_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6334785.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNNIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(6452..6742)
                     /locus_tag="ACFM8U_001569"
     CDS             complement(6452..6742)
                     /locus_tag="ACFM8U_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334786.1"
                     /translation="MAKYFTETDIGIREHFDFFGEFVVSPAARSGDLALTYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVEGGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         1747 a         1817 c         1548 g         1641 t
ORIGIN      
        1 ttaacgcaaa tttatttaaa aaaatagact ttaaagaaag aaaatcaatc acatagaatt
       61 tctgcaaaca aagaaaaaag gaagccgcag cttccttttc aattttttgg attaagcagc
      121 caaagcagct ttggcttttt caaccaattg tgcaaaagcg gctttatcga acacggccaa
      181 atcagccaat actttgcggt cgatttcaat agaggcgcgt ttcagaccgt tcataaattt
      241 gctgtaagac aacccgtttt cacgcgtacc tgcattgata cggacaatcc acaattgacg
      301 gaattggcgt ttgcgttggc ggcggtcacg gtatgcgtat tgaccggctt tcattaccgc
      361 ctgcttggca acgcggtaaa cgtttttacg acgaccgcga tagcctttgg ctaacgcgaa
      421 gattttttgg tgacgggcac gggcggtaac accgcgtttt acgcgtggca tattctaaac
      481 tccttaagcg tagggtaaca ttttagcaac agaagccaaa tcgcgatcat ttaccataga
      541 ggtaccgcgc aattggcgtt tgtttttggt ggtcttttta gtcaagatgt ggcgtttgaa
      601 cgcatgagcg cgtttcacac cgccgttacc cagtacttta aagcgttttt tcgcgctaga
      661 cttggttttc attttaggca tgggaaaact ccattcgtta tcggataagg cattaggggg
      721 ttttaaaaca tcggtttcaa acacttgaac ccacaatgcc acggtttttt gccgcaatta
      781 aaaacttact ccgaagcgac aatcggagta agcggagaat tatagcttta ttttttcttc
      841 ggcgcaatca tcatcaccat ttggcgacct tccattttgg gaaaggactc gatttgcgcc
      901 acttcagcca aatcttcttt tacacgttcc aaaagttgcg cgccgagttg ctggtgagcc
      961 atttcacggc cgcggaaacg caatgtcact ttgactttat cgccgtcggc aaggaagcgg
     1021 ttgatgttgc gcatcttgat ttgataatcg ccctcatctg tacccggacg gaatttgatt
     1081 tccttgattt gtacctgctt ttgatttttc ttggcttcgt cgcgtttctt ggcctgctgg
     1141 tatttgtatt taccgtaatc catcagtttg cacacaggcg gtttagcagt tggggaaatc
     1201 tctaccaaat cgacatcctg cccttcggcc atagccaaag cttcacgaac tgaaacgaca
     1261 ccaagctgtt cgcctgactc actgattaaa cgcacttctt tggcggtaat ttcgccgttg
     1321 attcgtgctt cgcgttcttg agcgatgatg aatactccta taaaaattaa tgattgacga
     1381 gggcatcagt gatttcttgc tgcaattgcg caatgaaatc atccaaatcc aaagaaccca
     1441 aatcttctgc tttgcggcgt accgccactt tgttttcctg cttctcctta tcgccgacaa
     1501 cgatttgata agggaaacgg tattggctgt tgtcgcggat tttgtaaccg attttttcgt
     1561 tacgcaaatc cagctctaca cggaagccag cggcttgcaa tttagcagcc acttcccgac
     1621 aataatctgc ctgattttcg gtaatattca taattaccaa ttgaaccgga gccagccata
     1681 acgggaaaga gcctgcatgg ttctcaatca gaatgccgat aaaccgttcc aaagaaccta
     1741 aaatggcgcg atgcaacata acaggtcgcg cacggtcgtt gttttcggtt acatattcgg
     1801 cattcaaacg ctctggcaat acaaagtcca actgcagggt accgcactgc caagagcgac
     1861 ccaaggcatc tttgacatga tattcaatct taggaccata gaatgcgcct tcgccaggca
     1921 actcttccca ctctacgccg caagcggtca aggcatcacg cagcccttgt tcggcttttt
     1981 cccaaacttc gtcagaacct gcacgttgtt cagggcggag ggacaacttc acggcaacat
     2041 catggaagcc gaattgtttg taaatgcgaa ccagcaattc attgaatgca cgtgcctcat
     2101 caacgatttg atcttcggta cagaagatgt gcgcatcatc ctgtacaaaa ccgcgaacac
     2161 gcatcagacc atgcaatgca ccgcttggtt cattacggtg gcaagaaccg aactcagcca
     2221 aacgcattgg caaatcgcga tatgagcgca agccgttatt aaaaatttgc acatgacccg
     2281 gacagttcat cggtttcacc gcgtattcgc gtttttccga actggttacg aacatattgt
     2341 ctttgtagtt gtcccaatgg ccggattttt cccaaaaggt tttatccatg atttgaggcg
     2401 ttttgacctc tttataaccg gcggcgttca gttctttacg catatgctgt tcaatcactt
     2461 gccacaaagc ccagccttta ggatgccaaa acaccatgcc cggcgcttcg tcttgcagat
     2521 ggaacagatc caattgcttg ccaagcttgc ggtggtcgcg cttttcggct tcttcgatac
     2581 gttgaatata ggctttcaac tcgtcttttg tcgcccaagc cgtaccgtat atgcgttgca
     2641 gcatttcatt gttgctgtcg ccgcgccagt atgcgcccgc caacttggtc agtttgaagt
     2701 ttttcaggaa acgggtattc ggaacgtgcg gtccgcggca catatcgaca tattcctgat
     2761 gatgatacat ccccatcgct tccacttcgg gcatatcgtc aatcaggcgc agtttgtatt
     2821 cttcgccgcg ctcttgaaaa attttaatcg cctccgcacg cggagtcatg attttgacca
     2881 catcatagtc ttgggcaatc aattctttca tacgcgcctc aatagcggca acatcttccg
     2941 gtgtaaacgg tttttccgtg gcgatgtcgt aataaaagcc ctcttcaatg acggggccga
     3001 taaccatttt tgcattagga tagagttgct tgacggcatg cccgacaaga tgcgcgcagg
     3061 aatggcggat gatttcgatg ccttcctgat ctttcggagt aatgatttga acagcagaat
     3121 cttcaacaat cgggtcgcac gcatcgacca atttgccgtt taccttgcct gccaccgtcg
     3181 ccttcgccaa accggcaccg atagacgcag caatttgagc cacggtaacg ggggattcgt
     3241 attggcggac tgagccgtcc ggcaaggtaa tattcaacat caaaaagctc caaaacatta
     3301 aaaaataacg gcataaatgc cgttatggga atttggtagg cacgattgga ttcgaaccaa
     3361 cgacccccac catgtcaagg tggtgctcta accaactgag ctacgtgcct tcaaagaatt
     3421 tttgcatttt atcgggtcgt tttaattttt gcaagagata tgtgcatttc tttatgcaac
     3481 attgcggcaa gatttgggga tgccgtctga aaaaccgtgg gaaatcattt ataatgccgt
     3541 cttttaaaat atatttcgac ccctgtcgcc caactgacat tcaatcttat gcttaaattt
     3601 actttacaca aaaaagacgg atacgcccga cgcggcacgc tggagttgaa ccacggcaaa
     3661 atcgaaacac cggtatttat gccggtcggc acttacggtt cggtcaaagc gatgaacccg
     3721 caaaacctgc atgacatcaa agcacaaatc atcttaggca acacttacca tctgtggctg
     3781 cgcccgggtt tggaagtcat cgaacaattc ggcggcctgc acgaacttat cggttgggat
     3841 aagccgattt tgaccgactc aggcggtttc caagtatttt cgttgtccga catgcgtaag
     3901 ctgactgaag aaggctgtac attccaaagc ccgatcaacg gcgacaaact gttcctctcg
     3961 cccgaagtgt cgatgaaaat ccaaaccgta ttgaattccg acatcgtgat gcagctggac
     4021 gaatgcaccc cgggcgaagc aacgcacagc caagcgcaaa aatcgctgca aatgagcttg
     4081 cgctgggcag aacgaagcaa aaaggctttt gaggacctca aaaacccgaa tgcgctgttt
     4141 ggcatcgtac aaggcgcgat gtatgaagat ttgcgcgaag aatcgctgaa aggcttggaa
     4201 gagctcgact tccctggctt ggccatcggc ggcttgtccg ttggcgaacc caagcccgaa
     4261 atgtaccgta tgttgcgcgc tgtcggccct atcctgccgg aacacaaacc tcattattta
     4321 atgggtgtcg gcacgcctga agatttggtg tacggcgtgg cacacggcgt ggatatgttc
     4381 gactgcgtca tgcctacccg caatgcgcgt aacggctggc tgtttacccg ttttggcgat
     4441 ttgaagatca aaaacgccaa acacaaactc gacaagcgtc cgattgatga aagctgcacc
     4501 tgctacgcct gtcagaattt cagccgctcc tacctgcacc atctgcaccg tgtcggcgaa
     4561 atcttgggtg cacaactcaa caccatccac aacctgcact tctaccaagt catcatggca
     4621 gaaatgcgcg aagccataga acaaggcaaa tttgccgact ggcaggcgcg attccacgaa
     4681 aaccgcgcac gcggtgtgga ctgactcccg gcaatgccgt ctaaagccgc ttcagacggc
     4741 attttgtcgc tttaccctac ccgcactatt tttcagaaac cgatcatgct tccattcctt
     4801 ccagaaccct ccctttccta tacgcaacaa aaccgtaccg ccgttttgct gttgaacctc
     4861 ggcacacccg acgcgccgac cgcgcaggcg gtacgacctt atctgaagtc ctttctgacc
     4921 gaccggcgca tcgtcgaact gcccaaatgg ctgtggtatc ccattctgca tggtctggta
     4981 ctgacgttgc gccccaaaaa aagcgcgcac gcctatgaaa aaatctggtt taaggaaggt
     5041 tcgcccctag aagtttacac cgcacgccaa gctgccgcgc ttgccgaacg tatgcccgac
     5101 ctcatcgtcc gccacgctat gacctacggc aatccttcgg tggcagacgt attgtcggaa
     5161 ctgaaggcgc aaggcgcggg cagactgctg gtcatcccga tgtacccgca atatgccgct
     5221 tccagcagcg gagcggcggt agataaagtg tgcgaacaat tactgctgca acggaaccaa
     5281 atgagcgtcc gcaccgtttc gcgcttttat gacgataccg gctacattga tgcgatgaaa
     5341 aaccacatcc tccgatattg ggcggaacac gggcgcggga aaaaactgat gttgagtttt
     5401 cacggcgtac cgcagaagca ccacgacctc ggcgacccct atcccgacga gtgccgccac
     5461 accgccaaac tgcttgccga agcactggaa ctgaccgaag accaatatgt cgtatcgttc
     5521 caaagccaat tcggcagggc aaaatgggtt acaccgagca cgcaggattt gttcggcaaa
     5581 ctgcccaaac agggggtaac cgagctggac gtattctgcc cgggcttttt ggcagactgt
     5641 ttggaaacca tggaagaaat cgccctgatg gggcgcgaac agttttacga agcaggcggc
     5701 aaaagctacc gctatatccc ctgcctcaac gacaaccccg actggataga cgcactcgtc
     5761 gcacttgccg aagaaaacct tggtggctgg cgttgaaact gtttgaccca tgtctaaaat
     5821 gccgtctgaa cgccgtgttc agacggcatt ttcccgctac tttagcgtat gatggcatct
     5881 atgccggaca acaacatcaa gtgcaacctt actaccgatt cggcaagaaa atctttcacc
     5941 cgctacgcaa aatcaacaac aaggtttcaa acattaagga aacaccaatg aacacaaccc
     6001 gactgccgac cgccctcgtc ttgggctgcc tctgcgccgc cgcttctgcc gccgacaaca
     6061 atatcatgac aaaagggcaa aaagtgtacg aatccaactg cgtcgcctgc cacggcaaaa
     6121 agggcgaagg ccgcggaacc atgtttccgc cgctctaccg ctccgacttc atcatgaaaa
     6181 aaccgcaggt gctgctgcac agcatggtca aaggcatcaa cggtacaatc aaagtcaacg
     6241 gcaaaaccta caacggattc atgcccgcca ctgccatcag cgatgcggac attgccgccg
     6301 tcgccactta tatcatgaac gcctttgaca acggcggcgg aagcgttacc gaaaaagacg
     6361 taaaacaggc aaaaggcaaa aaaaactaaa cagacaaaat gccgtctgaa accggcaatc
     6421 cggcttcaga cggcattcaa atcaaacctt tctaacgcgg tactttcagc cccatagacc
     6481 cgatattgcc gtccccgtca acctccttcg cggtcagcat aaagccgccg atgtcaatac
     6541 ggccgccctc gaccggctcc tgactatcgg aacgcttatc gaaaagctcg gcaaggctca
     6601 aaccctcttc gcccgcttcc agcctcaaac cgtaagtaag tgccaaatca cccgaacgtg
     6661 ctgccggcga aacgacaaac tcgccgaaga aatcaaaatg ctcacggatg ccgatatccg
     6721 tctcggtaaa gtatttcgcc attttatagt gaa
//
LOCUS       DAXHSJ010000117         6634 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000117 DAXHSJ010000000
VERSION     DAXHSJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.117"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            133..1500
                     /locus_tag="ACFM8U_001570"
     CDS             133..1500
                     /locus_tag="ACFM8U_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC6334787.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            1541..2041
                     /locus_tag="ACFM8U_001571"
     CDS             1541..2041
                     /locus_tag="ACFM8U_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFC6334788.1"
                     /translation="MTATYQLVDNEKLALLKNTDTEWQELFEELEDLEDEAAVCLDID
                     KMWDVLHFVLTGTSAENPLKGNLLSESIVGTAPISDDDGYAAYIESAQIHEIISALEG
                     FDIKSAMEHFSMQVCKEHHIYPDIWDYEDEIYDIKEEITDYFYKIGNFYKTARHSNSN
                     VLVSIY"
     gene            complement(2469..2915)
                     /gene="smpB"
                     /locus_tag="ACFM8U_001572"
     CDS             complement(2469..2915)
                     /gene="smpB"
                     /locus_tag="ACFM8U_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC6334789.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKEAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(2970..3980)
                     /gene="waaF"
                     /locus_tag="ACFM8U_001573"
     CDS             complement(2970..3980)
                     /gene="waaF"
                     /locus_tag="ACFM8U_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC6334790.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPAKHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(4111..4186)
                     /locus_tag="ACFM8U_001574"
     tRNA            complement(4111..4186)
                     /locus_tag="ACFM8U_001574"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4151..4153),aa:Asn,seq:gtt)
     gene            complement(4377..5129)
                     /locus_tag="ACFM8U_001575"
     CDS             complement(4377..5129)
                     /locus_tag="ACFM8U_001575"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC6334791.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            5440..5964
                     /locus_tag="ACFM8U_001576"
     CDS             5440..5964
                     /locus_tag="ACFM8U_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFC6334792.1"
                     /translation="MQTRILSAVLLAFSTTAFAGGAFTLQFDNPSEDGGFTQNQFLSA
                     PYGFGCSGDNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHWVVADIPADVRRRN
                     ATSLQLSRCASIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANMPQSAASA
                     ALCGTSSSASTAAA"
     gene            5925..6104
                     /locus_tag="ACFM8U_001577"
     CDS             5925..6104
                     /locus_tag="ACFM8U_001577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334793.1"
                     /translation="MRHFFIRQYRSRIIKRRAHTPGIRQLYRIAHCLSRPVLCGEPFY
                     GFLFESNPLRLIEAV"
     gene            6118..6270
                     /locus_tag="ACFM8U_001578"
     CDS             6118..6270
                     /locus_tag="ACFM8U_001578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334794.1"
                     /translation="MTSNVCPPANATASPAAILNSRLAHRSFLLPRRTISTADSYTAT
                     LRYKAV"
BASE COUNT         1726 a         1935 c         1480 g         1493 t
ORIGIN      
        1 ctgctgccga agctgcaaaa taagcatttt ccgcttgcaa aaaagcagga tacgttcagt
       61 atcctgcttt tttgattttt cagacggcat cagattccct tcctcaatct tctccctacc
      121 cttccgacaa acatgcctga ccttcatacc gaattttccc gactcctgcc ggcagatgaa
      181 attgccgaac tttctccgac gcttttaaaa gaccagcgca accgctttac gtctgcacca
      241 gacatcattt tgcagccgcg cagcgttgaa agcgtgcaaa ccattatgcg tttttgttac
      301 gaacaccgca ttccggttac gccgcaaggc ggcaataccg gtttgtgcgg cgcggcagta
      361 tcggaaaacg gcgtattgct gaacctttcc aaactcaacc gcatccgcag catcaatttg
      421 tcagacaact gcataaccgt cgaagcaggt tccgtactcc aaaccgtcca acaggcagcc
      481 gaagcctcaa acaggctgtt tccactcagt ctcgccagcg aaggctcgtg ccaaatcggc
      541 ggcaacatcg cctgcaatgc cggaggtttg aacgtattgc gttacggcac gatgcgcgac
      601 ctggttatcg gtttggaagt cgtcctcccc aacggcgagc tggtttccca tctccatccc
      661 ctgcataaaa acaccaccgg ctacgacctg cgccatctgt ttatcggcag cgaaggcaca
      721 ctgggcatca tcactgccgc cacgctcaag ctttttgccc gtccctcgga caaggcaacg
      781 gcatgggtcg gcatacccga catcgaatcc gccgtccgcc tgctgaccga aacccaagct
      841 cactttgccg aacgcctatg cagttttgag ctgatcggcc gttttgccgc cgagttgtct
      901 tccgaattca gcaaattccc cctgccgaca cattcagaat ggcatatttt acttgagttg
      961 accgactcat tacccgacag caatcttgat gatcggcttg tcgaatttct ttataaaaaa
     1021 ggatttaccg acagcgtatt ggcacaaagc gaacaagaac gtatccatat gtgggcattg
     1081 cgcgaaaaca tctccgcatc gcaacgcaaa ctgggcacca gcatcaaaca cgatattgcc
     1141 attcctatcg gacgcgttgc cgactttgtc cgccggtgcg ccaaagattt ggaacagaat
     1201 tttaaaggca tacaaatcgt ctgcttcgga catcttggcg acggcagcct gcactacaat
     1261 actttcctgc ccgaaatcct cagcaatgaa gtctatcgtt acgaaaacga catcaacgac
     1321 gcagtttatc gcaacgttct tgcctgcaac ggcacgattg ccgccgaaca cggcataggt
     1381 atcatcaaaa aacagtggct ggacaaagta cgcacgcctg ccgaaatcgc cctgatgaaa
     1441 agcatcaaac aacaccttga tccatataac attatgaatc cgggcaaact gcttccgtaa
     1501 ataactgatt caaaatgaaa ttataggaaa tatcatggga atgacagcaa cctatcaact
     1561 ggtcgataat gaaaaactgg ctttgcttaa aaacaccgat accgaatggc aagaattgtt
     1621 tgaagaactg gaagatttgg aggatgaggc tgccgtctgc ctcgacatcg ataaaatgtg
     1681 ggatgttttg cattttgtac tgaccggaac aagtgcagaa aatcccttaa agggcaatct
     1741 gttatccgaa tcaatcgtcg gaacggcacc catttcggac gacgacggat acgccgctta
     1801 tatcgaatcc gcccaaatcc atgaaatcat ctcagctttg gaaggcttcg acataaaaag
     1861 cgcaatggag catttcagta tgcaggtgtg caaagagcat catatctacc ccgacatttg
     1921 ggattatgaa gatgaaattt atgatataaa agaagaaatt accgactact tttacaaaat
     1981 cggcaatttt tacaaaaccg cccggcattc aaatagcaat gtactggtca gcatttacta
     2041 aatatatttc aaacatcacg gaaactttaa tgaagacaaa gtacggaatc gcactcaagg
     2101 aaacctaggt atttcaacct gggagaccat catgagtaaa agttacagaa aacacccctt
     2161 ttcccctata accacggcag tcagcgaaaa acaggacaaa cgccttgtca accggaaact
     2221 acggagaata gcgcgcgaat gtctgaaaca aggaaaagaa ccaccccacc atatcagggc
     2281 agtcagcaac gtttacgatt tcgccaaaga cgggcatttc cgtttttccg cttcccgtca
     2341 tccgcatctt ttacgcaaat agaaaacagg gcaaccgccc tgtttttccg tttcgtattt
     2401 caacaccatt gttcaaacaa ccgtcagacg gcattgttca aacgccgtct gaaaccgcat
     2461 cccccccatc agcgcgtatg cttaatcaac cgctgtttct cgcgtttcca atccgcctct
     2521 ttcatactct gacgtttgtc gtgctgtttc ttaccttttg ccaaaccgat ttccatcttg
     2581 atttttccgc gcgaaaaatg caaatccagc ggcacgatgg tgtagccggc acgttcggtt
     2641 ttgccgatta atttgttgat ttccgactgg ttcaacaaga gcttgcgcgg acgtacggca
     2701 tctggtttaa tgtgtgtcga ggctgtgggc aaagccgtaa tatggcagcc gaccagataa
     2761 aacgcttctt ttttccaata gatataactc tctttaagct gtacgcgcgc ggcgcggatt
     2821 gctttgactt cccagccttc caagaccaaa ccggcttcaa tccggtcttc aatgaaaaaa
     2881 tcgtgaaatg cttttttatt gttcgcaata gccataaaca tcctatcaat atccgccgtc
     2941 agacggcata aacccgaaaa cagaacccat cataccgcct cttcaaccgc ctgcacaatc
     3001 ttctcgggat acagcctgtt gaggcagtcg gtatgcccca gcggacattc ccgcttaaaa
     3061 cagggcgaac attccaagtg caggctgacg attttcgccc tgtcgctcaa aggcggcgta
     3121 tgcgtcgggc tggaagaacc gtaaaccgcc accaccttcc tgcccaatgc cgccgccaag
     3181 tgcatcagcc cgctgtcgtt gcacacgacc gtgtccgcca acgacaacaa atccattgcc
     3241 tgcgacaaat cggttttgcc gcacaaattg acacacatac cgtctgaaag gcagttgatt
     3301 tcctcggcga tttcatcatc tttttgcgaa ccgaacaacc aaacctgcca acccgccgcc
     3361 agataatgtt tgcccaactc ggcaaaatgc tttgccggcc aacgctttgc cggcccgaat
     3421 tccgcacccg gacaaaaagc cagaacaggc tttccaagat tcaaaccgaa agtttcgaca
     3481 gaaatttccc gccgccgttc atcaatggaa aactcgggga atcccgaatg cccgtcaaaa
     3541 tcttcctgac tctgatgcgc gagagccgta tatcgatcca ccatcaaagg cagacgttcc
     3601 ttatccagcc tgcgtatatc gttcaacaga aaataacggc tttcaccgac ataacccgtc
     3661 cttttaccga tacctgtcgc cagcgcgatg actgccgact tcaaagaacc cggcaacaca
     3721 ataacctgat cgtatccgcg ccgccccaaa tccctgccga cccgccagcg gcgtttcaac
     3781 tccaacgcac cgtgtccgaa cggattctca agaatttcat tcacttccgg catacgctcg
     3841 aacaccgcca tcgaccactt cggcgcgaac acatcaatcg tgcaaccggg gtgaagttcc
     3901 ttcaaacggc ggaacaaggg ctgggtcatc acgcagtcgc ctatccaact gggagaaata
     3961 atcaggattt tgatggacat aacaagaaac cgaaatcaga caggcggaat tttaccgcga
     4021 aaccgttgga aaacctatct cgccgcattc cgaacgccgg acgtgcaaat atgaaaaagc
     4081 ccgaacattc aagttcgggc ttcaaaattc tggctccccg acctgggctc gaaccaggga
     4141 cctgcggatt aacagtccgt cgctctaccg actgagctat cggggaatgg ggcgtattat
     4201 agcgtccgga aaaaatgtgt caatccttaa ttttggaaaa atgggcgaca aaacgacaag
     4261 catatgaatc agaaagacat taagaccgat gccttaaaag gattgccgtt gtatgaattt
     4321 ccacagccgt catcacacca tatttaagcc cgatgagccg ttctgccctc ccccgcttaa
     4381 aacaatgccg tctgaacttc gccgcgttcc aaagccagta aaaactgttt gcggttcaac
     4441 ccgcccgcgt agccggtcag tttgccgtcg ctgccgatga cgcggtggca gggaatcagg
     4501 atagatacct tgttctgccc gttggcggcg gcaacggcgc ggacggcttt ggggttgccc
     4561 aaacgctgcg cctgctcctt gtagctgcgc gtttcgccgt aaggaatcgc caagagcgcg
     4621 tcccatgcct gcttttgaaa ctcggtgcca atctgctcca aaggcgtggc aaaggttttc
     4681 agacgaccct tgaagtataa gtccaattcc tgccgcaaaa gttgcgtccg ctcatcctcc
     4741 cgaaacacaa accgtccgcg caaggctttt tggacggcgg caatttcctg ttccaaatgc
     4801 ttctgtccga caaactccag cagacacaat cccttatttc cgaacaccgc cagcatctcg
     4861 cccaaaggcg tggcaatggc ggcacacacc agctcgttca aactgtcagg ataacgcgct
     4921 tccaacaaac ggatggcgcg gcggatgcgg acatattctt caggcgcgca gccgatattg
     4981 tcccaaaaat cccgatcgaa ctgcccggct tcgcattccg tcaaattggg atgcggcata
     5041 acgccgcact caaaaacccg ggtttcgagg caatggcgga tttcatccca ttttgacggc
     5101 aggttgttta aggaaggcag ggtaatcatt cgtttgctcc gtatctctat catggatttg
     5161 gcggcaaaat ccccgatttc cgccattccc gcacgccgat gcaggaacgg gctatgacgt
     5221 aaatcttgag ggttgggttg cggcaatgcc taaatatgcg atatttctaa agcctcaggg
     5281 aaaggaatta aagacttcag acgacctgca aagggtcgtc tgaaaccacg atttttgcat
     5341 ttgcgcattt cggcacatca tccaaccgtt tcggcacatt cctgccgccg ttgacagcct
     5401 ataatgaatc cacttattca tcaagcaaag gaatcatcta tgcaaacccg catcctctcc
     5461 gccgtactgc tggctttttc aaccactgcc tttgccgggg gcgcattcac gctgcaattc
     5521 gacaacccgt ccgaagacgg cggcttcacg caaaaccagt ttttgagcgc gccttacggc
     5581 ttcggctgtt caggcgacaa tgcttcgccc gcgctgtcgt ggaaaaatcc gcccgccggg
     5641 acaaaaagtt tcgtcctgac cgtttacgat aaagacgcgc cgaccggact gggctggatg
     5701 cactgggtgg tcgccgacat tcccgccgat gtccgccgcc gcaacgcgac ctcgttgcaa
     5761 ttaagccgct gcgccagcat cgccgacgac cagtccgcag ccatatcggc ggtaatcagt
     5821 ttgcggattt gccgcatcag gttgacgcct tcgtacacgg caaaaccgat gccgtcatgc
     5881 tgcaaccacg ccaacatgcc gcaaagcgcg gcctccgcag cattgtgcgg cacttcttca
     5941 tccgccagta ccgcagccgc ataatcaaac gccgcgccca tacgcccgga atacggcagc
     6001 tttaccgcat cgcacactgc ctgagccgtc ccgtattgtg cggcgaacct ttctacggtt
     6061 tcctgttcga aagcaatcca ttgcgcctga tagaggccgt ctgaatcggg aatattgatg
     6121 acgtcaaacg tctgcccgcc tgccaacgca accgcctcac ccgccgcaat cctcaattcg
     6181 cgcctcgccc accgcagctt cttgcttccg cgccgcacga taagcacagc cgattcatat
     6241 accgccacgc tgcggtacaa ggcggtatga tgttgcacga tgccgcctaa agcacccaat
     6301 cgttcgcgtg tatgaaagta atttgacggc actattatct ccaacacgca gggccgcaca
     6361 taaggcgcag ccctgtttac cctgatttgg aaggggttac ttcccaaaaa taaaccaaaa
     6421 tcccactgcc ccgaatggcc gggtttcaac catattccgt cggtgtgcct gcaacttgac
     6481 tccctcccct gtgggggagg gttggggaga gggtaaaacg gggcagatac agacaatatt
     6541 tccgttaccg ccccgatgcc ctctccctag ccctctccca cgaaagaggg aatggattgc
     6601 cgtttaaata aaaaaatcta cataaaaatc aatg
//
LOCUS       DAXHSJ010000118         6492 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000118 DAXHSJ010000000
VERSION     DAXHSJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.118"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..181
                     /locus_tag="ACFM8U_001579"
     CDS             <1..181
                     /locus_tag="ACFM8U_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6334795.1"
                     /translation="YLSKTRYVVEQSFGTLHRKFRYARAAYFGLLKVSAQSHLKAMCL
                     NLLKAANRLSAPAAA"
     gene            complement(457..669)
                     /locus_tag="ACFM8U_001580"
     CDS             complement(457..669)
                     /locus_tag="ACFM8U_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC6334796.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(681..962)
                     /locus_tag="ACFM8U_001581"
     CDS             complement(681..962)
                     /locus_tag="ACFM8U_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334797.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(1009..1212)
                     /locus_tag="ACFM8U_001582"
     CDS             complement(1009..1212)
                     /locus_tag="ACFM8U_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334798.1"
                     /translation="MNIIEQEMKHSSLRKVDAEIAKIIADAHKINAESVKIAQESRWY
                     PMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1292..1864)
                     /locus_tag="ACFM8U_001583"
     CDS             complement(1292..1864)
                     /locus_tag="ACFM8U_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFC6334799.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            complement(1839..2183)
                     /locus_tag="ACFM8U_001584"
                     /pseudo
     CDS             complement(1839..2183)
                     /locus_tag="ACFM8U_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2176..3051)
                     /locus_tag="ACFM8U_001585"
     CDS             complement(2176..3051)
                     /locus_tag="ACFM8U_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HFC6334800.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(3096..3386)
                     /locus_tag="ACFM8U_001586"
     CDS             complement(3096..3386)
                     /locus_tag="ACFM8U_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6334801.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3398..3601)
                     /locus_tag="ACFM8U_001587"
     CDS             complement(3398..3601)
                     /locus_tag="ACFM8U_001587"
                     /inference="COORDINATES: protein motif:HMM:TIGR04111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6334802.1"
                     /translation="MLSQWERAIYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYL
                     VVGGQRKGNYGKGHEIAKKLGLK"
     gene            3691..4068
                     /locus_tag="ACFM8U_001588"
     CDS             3691..4068
                     /locus_tag="ACFM8U_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6334803.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            4097..4342
                     /locus_tag="ACFM8U_001589"
     CDS             4097..4342
                     /locus_tag="ACFM8U_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334804.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4576..4725
                     /locus_tag="ACFM8U_001590"
     CDS             4576..4725
                     /locus_tag="ACFM8U_001590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334805.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLT"
     gene            4741..5544
                     /locus_tag="ACFM8U_001591"
     CDS             4741..5544
                     /locus_tag="ACFM8U_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334806.1"
                     /translation="MDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQ
                     APPSGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNI
                     EFKAVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSE
                     LSDEELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQG
                     EVIQPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            5541..6023
                     /locus_tag="ACFM8U_001592"
     CDS             5541..6023
                     /locus_tag="ACFM8U_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334807.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            6113..6283
                     /locus_tag="ACFM8U_001593"
                     /pseudo
     CDS             6113..6283
                     /locus_tag="ACFM8U_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
BASE COUNT         1558 a         1508 c         1610 g         1816 t
ORIGIN      
        1 gtatttgtcg aagacccgtt atgtggtcga acaaagcttc ggtacgctgc accgtaaatt
       61 ccgctatgcg cgggcagcct atttcgggct gctcaaagtg agtgcgcaaa gccacctgaa
      121 agcgatgtgt ttgaaccttt tgaaagccgc caacaggcta agtgcgcccg ctgccgccta
      181 aaaggcagcc cggatgcctg attatcaggt gtccggggag gattaagggg gtatttgggt
      241 aaaattagga ggtatttggg gcgaaaacag ctgaaaacct gtgtttgggt ttcggctgtc
      301 gggagggaaa ggaattttgc aaaggtctca gagtgagttt attttggggc ggcggcaggt
      361 cgggggcaag cggcgtgggg cttggttgtg gtttttaggt ttgggggtgg gtaaaaaatg
      421 ccgtacccgg cgggcaggct tcgcactctg aaggtttcag acggcgtttg ttttttctgt
      481 ccaatcgagg aactgccgcc atttttccag cggcatatcg gcccgccgcg tttcggtatc
      541 gggttcggct tcccagcggc ctacctgtat gtagtgcttc accccgacga tttgcgccaa
      601 ctcggcctgt gtcagtttgc agcggttgcg cagggtgcga aggttgtaag gcgtgtagcc
      661 gagttccatg tcgttgcggt ttattttgtt tcgcatattt ttttgactgc ccggcggcag
      721 gtttcggtaa ggacggcggc aaatcgggct tcgtctgccg gttttccgtc gaaaaaaata
      781 aaatcgtaaa gggtgatgcc gttttcggaa tggcggtaat gcccgcccgg gcattcgctg
      841 tctgtctgcc tgtggatttt ggcaatcagg cggggatagc ggcaatgggt aatgaaacgg
      901 cttgcgccgt cttgcccgat aatccattcg gggtagcggt tgaatagtgc ggctctgttc
      961 attttgttcg ggttgttcgg gggataaagc ccctcgcggg gcttgtggtc aggcaaattt
     1021 gaatatcagt gccaacacgg cggcgatggc ggtaaccagc ccggtggccg ccatcatggg
     1081 ataccatcgg gattcttggg ctatttttac ggattcggcg ttgattttgt gcgcgtctgc
     1141 aatgattttg gcgatttcgg catctacttt tctcagactg gagtgtttca tttcttgttc
     1201 gattatgttc atcgtacttc ctttcgtttt tggcggttgc cgccgcttgt cggatggtag
     1261 gatgtctgcc atgtgtatat attgatacct tttaggtttt attgcaagtg ttttgggcgg
     1321 cggcttcgta tgcttggcgg tggcggcggc tgtaccattc ggcgagttcg cgccgctctt
     1381 ggaggcggta gctgtcggcg gtcaggtagt ggtgcaggct tgagaagccg gcgcgttgga
     1441 gggcggcgcg gctgatgtgg ccgagggcga ggtctgttgt gagggtgtct tttccggcgg
     1501 tcaggctgat gttggtgaag aggtggcgga atccgtgcat tgtgtgtttg gatttgccgg
     1561 gggtgctgcc gtcatagccc agtcgtcgga tggcgttgtg ggcgaatttg atgctgatgt
     1621 ggtcgggatg gggggcgggt ttgcgccgtg ggcggatgcc ggggaaaagg tggatgttgt
     1681 cgccggtctg tgtgtgcagc tctcggagta tttctaccgc ccagtccgac agtgggacgg
     1741 taaaggggtg tttggtcttc atgtcggcgg cggggatgtg ccataaccgg gcggtgaggt
     1801 cgatgtcttg ccagcgggcg gaaagcagtg cggagggacg ggatgacttc cccggtttcc
     1861 ccatcaatcc gcgccgcctc gatttttccg ttggcgcgca gcatttccac cgcctgccgc
     1921 accgacatca accgcttgcc gttgcgcctc atcgcctcca ccaaaaccgc cgaaatcaat
     1981 ttggcttctt ccggtttaag ctccgtcttg cccgcatcgc tgcgccgttt gcgcgtcggc
     2041 ttgacgctga ccgcctccag cttgcggtat aacgtggcaa ggctgatgcc caattcctgc
     2101 gcctgctgct taagatatgc agagcgtgcg ccgcgttcca ttgcttccgc ctgattctcg
     2161 actgccttaa gacgctcaat cattgccgga ttcatcgcct tctcccgttt caccgcccaa
     2221 ccattccggc acattgtctg tcggtgcttc agtcggcagg gcatagcttt cgcgcagttg
     2281 ctcgcagtcc aaaataattt gattgagcgt gccgaccatc tttgcctgat ggctgatccc
     2341 gtgtgcctca ctgtgcgcat taagttggtc gaacaaatct ttcagacggc tcacttgact
     2401 gcggataccg acctcaaggc ttgttaactg catcgccaac tcgctgccta cgtcttccgc
     2461 cttcggctct ctgacaacgg tttgcttctt agccagcttc tcggccagct catcaatttt
     2521 ggctgttttg gttttcatca cttcgtcttt ggcggcgagg ttttcgcggc tttcgcgcag
     2581 ggcgacgcgc agctcgcgca ccgtcattcg gtccacatcg tcaaaggtca tgccgttgac
     2641 ttcttcccct tcggccaaac ccaccagcgt aacgtcttct tcgaccagca gctcaagcag
     2701 cttcgacttg cccaaatcca tcagcttcgg cgcggctttc tgcatttgcg gggtcgcaaa
     2761 gcggcgagtg gctgacatca gacgtgatgt ttctgcgata cccagcccaa attggctttt
     2821 cacaatttcc ataaaccgtc cgtgttccgt atgctctttt aaaataatca gcgcacgtcc
     2881 cagttcgaac atgccttcca tcgtctgccg tactgcttga cgaccgcgtt caacccaacg
     2941 ctcttcgcta taagtctcgc cgttacccca ctgctccatc accagcacac tgcgcatcgc
     3001 tgcctgattg cttactttgt cggttgcgat aatttcaatt tctgtattca ttttttatct
     3061 ccaaagtttc cgacgtcgga aactttcaaa atccgttaat ccacatcgac ccgcttgccg
     3121 atttcggcaa tcttgctttg cagccgttca tgctgctgcc tgaaccgctc tgcgatttgc
     3181 agggttttga tgccgtaggc gtagttgccg ttttcaagtt tgatgaccaa tcccgaggca
     3241 accaaatcat caatatccct gctgacttgc gatggcgtca gccccagtcc gaccgataaa
     3301 tccttattgc tcagaccgat aatcggatgc tcgtcaagcg cgataaagac cctcaatagc
     3361 cgttgtaccc ttttactttc tgccatccgc atcctcctta tttcagtccc agcttcttgg
     3421 caatttcatg ccccttgccg taattgcctt tgcgctgtcc gccgaccacc agatacacat
     3481 cgcgcggctt aaagccgttc tctcttgccc actgcgccag cgtttggccg ttttgggcaa
     3541 aattttcttt tagtttttca acagtatagc catagatagc cctttcccat tgtgataaaa
     3601 tagtgaacta tttatgcact agaaattaat tagtgcatta ccaatgcgtg aattatgaga
     3661 ttttaaaatc tcttttacaa ggattatttt gtgatttcta aatctctttt tggtaatcgt
     3721 ttgaaagaaa aaagaaagtt tttagggctt acccaagcac aagctgctga aaaagcaggt
     3781 attgagcgcg aaacatgggg caaatacgaa cgtggcgtat ttatgcctag tggagatgta
     3841 ttgctctctt ttttgaatat gggaatagat gtgagcagct tatttgctgc tgaacaggga
     3901 attaggccgt ctgaaaattc tgaaattagt aaggaagagt cagagttatt agggtattac
     3961 cgccaagcca gcgataacgg caaatttgtc attctcagtg tggcaaaagg cgcagagaaa
     4021 aaagcagccg aaaatactca agtttcagcc acgcataaaa cagcgtgatt tttaaatcca
     4081 aaatggagac agtcttatgt ttgactttga acaacaaatc aaatggggtg agcgagcgga
     4141 agaaatcgtt aaagaggcag ccacccagaa caatatcgaa attcccgaac ctttggcttc
     4201 ggcattagct aaggcggtaa aagtgcatta tctaagccaa gctggtgtgt tttctttggt
     4261 agaagcctat gcagacacgg taaatccaac tgaaaaagaa gtagattacc aagcaatcgg
     4321 caaagagcta tttgaaaaat aaaaaatgcc gtctgaaatt ttcagacggc atttaggaca
     4381 ctccgacagt cgggctgctc cggtgttctg aaacggatat taagcaattg aagcggccgt
     4441 gcattttaaa acgcattaaa aactaccccg acatacaggt catcgggcag gtggttcagg
     4501 tgttgaaaga tttgaattga tgccgtctga atttattttt tcgacacaga tattaaaaga
     4561 gaaaggactg tacaaatggc aaaaaaaact actaaaacta ttcattattt acgtgcgcag
     4621 caaaaccata gcttgttcga tttagaggta gcattaagaa gggttttgtc agctgctcct
     4681 actgttcaag atacacaaat tgaacaatac aatcagctta cctaaatcat gcatagaact
     4741 ttggatccta aagatgtaaa aggcaattca attggtggat tactgataca cattggcagc
     4801 ggtacaaaag atgaacatat ccgcactatg agcaatagac ctgtacagca agatgatcat
     4861 ggtggaacac aagctcctcc aagtggatat tcctttttgc gtaaagaggc ttttttgtat
     4921 attgttggac accatgtaat tttttgcgga catggttttt tgtcagcatc aacggttgct
     4981 tcttatttaa gtttacttag caataaattg agagagaaca atccggatat tgttcaattt
     5041 aatattgaat tcaaagcagt aggcaattgc gacaagttgt cattgataca acaacatggg
     5101 gtaaaaagta ttcttttaga tgcttcggca tatcaattat ccagaaacag actgtatcaa
     5161 gatagccgtt caaccattgc taaagcatta gggaaagttg gtagtgtatt tacaagcgaa
     5221 ttgagtgatg aggaattgga agcacaatcc gaaattcata tcaatttaga agtcttgcta
     5281 aacggtaatt cgagagccag tattgaagcg caatcgctga tgcaggagca ggcagaggaa
     5341 attattgatg atgaaacggt taatcaagga ttttccatta ccacgcaaca aggtgaagtg
     5401 attcagccct ctgatgtaaa actgtctaaa tctgttagaa taggacgata cgatgaagcc
     5461 aactcgttac tccctgactc ggcatttaca gcaatcagtg aatatttcct agaactccaa
     5521 agcagaaact taactgaaca atgaaaaaat atctgccctt actacgtttt ttgttgattg
     5581 cttgtatatc agcattttta gcatggaaag ggcaaccttt tgttcatggg aacgagaagg
     5641 ccgttgattt aattattaac gtatttgcaa tcttagccgg ttttttaatt gccattatga
     5701 cattgtttag tgatatgagg tttgatgaag atgcaaattg gcgacaaatt caaattcgtg
     5761 aaggcgtaca agaacaacgg tatataaaac attccttact tttttacaca tatcttgccg
     5821 tattagtttg tgtttttatt gtcatcttgt tggctcataa agaagaatac aaaaatggac
     5881 cagccatttt ttggcttgaa cggagctatt tatttttagc ctgtatttcc attttttatt
     5941 ctgtattctt acctgggaat ttaattaaaa gccgaaaaga agagtttaaa aaattaatgg
     6001 aaaagaagaa acccaaaatc tagaacgagt tttaaccccc tttaaaatcc cattcagacg
     6061 accttttcta aaatccctgt attgattttt aattcaatac agggattttt ccatgtcaga
     6121 caagttcaac caattcatca accgcgtcct ctctcacgag ggtggttacg ccaaccatcc
     6181 caaagacccc ggcggcgaaa ccaattgggg catcactaag cgcaccgcac aggcaaacgg
     6241 ctacaacggc tccatgcgtg ccatgacgcg tgaacaggca atcagagacc tttgcaaaat
     6301 tcctttccct cccgacagcc gaaaccccaa cacaggtttt cggctgtttt cgccccaaat
     6361 accgcctaat tctacccaaa tcccccctta atcctccccg gatacccgat aatcaggcat
     6421 ccgggccgcc ttttaggcgg caacaggcac acttagcctg ttggccgctt tcaacaggtt
     6481 caaacacatc gc
//
LOCUS       DAXHSJ010000119         6322 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000119 DAXHSJ010000000
VERSION     DAXHSJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.119"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..96)
                     /locus_tag="ACFM8U_001594"
     CDS             complement(<1..96)
                     /locus_tag="ACFM8U_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6334808.1"
                     /translation="MRKSRLSRYKQNKLVELFVAGVTARTAAELVG"
     gene            163..465
                     /locus_tag="ACFM8U_001595"
     CDS             163..465
                     /locus_tag="ACFM8U_001595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334809.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEMER"
     gene            complement(547..2634)
                     /locus_tag="ACFM8U_001596"
     CDS             complement(547..2634)
                     /locus_tag="ACFM8U_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFC6334810.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2637..3902)
                     /locus_tag="ACFM8U_001597"
     CDS             complement(2637..3902)
                     /locus_tag="ACFM8U_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC6334811.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVENGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLVEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(3947..4084)
                     /locus_tag="ACFM8U_001598"
     CDS             complement(3947..4084)
                     /locus_tag="ACFM8U_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334812.1"
                     /translation="MNTETIYATVFCILAATISGLLVKFNVIKIETSINSKFMLLGIS
                     I"
     gene            complement(5124..5360)
                     /locus_tag="ACFM8U_001599"
     CDS             complement(5124..5360)
                     /locus_tag="ACFM8U_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6334813.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
BASE COUNT         2021 a         1123 c         1091 g         2087 t
ORIGIN      
        1 gcctactaac tctgctgccg ttcttgcagt tacacctgcg acaaatagct caacgagttt
       61 attttgttta taccggctta gacgactttt tctcataggg ataattctaa cttaatttga
      121 atttccctag ttatctagga cagcccctta aaaaatatcc ctttgaaaga ttttgatgaa
      181 gtttgtgaaa agtgggagaa atcctttaaa gaaaatggaa aagaaattga atataatcaa
      241 aaatattcaa gaatttatgg tgaagttcgt ggagctggct ttaatggcga tatcggagaa
      301 aagctacatc gttgtgccgt gatggaatgg gctgaaaatg agaccgtgaa attagcccaa
      361 aaatgggaac aagagcagaa aaaacaacaa attcaacaga aaaaggaaac tgaaaaatcg
      421 ccaaaacaca aagccagtcg tgatgattgg gaaatggaac gttaaaccgc tattttcatg
      481 tattctatta aattatttga aaaaagactt cactttttgt taaagaaaaa tgaagtcttg
      541 ttctgttcaa cctaaattca gaattttatc tgccgattga attgtttctt ggcggtgtgc
      601 aaccattatt ttcataatac caagactttt taggttatgg ttaatttttt gttcattttc
      661 tacatctaaa tggctacttg cttcgtctaa aaatagaatt ttgggtcgtt tatacaatgc
      721 acgagccaag ataactctct gcttttgtcc acctgataag atatttccca tatcgccaat
      781 caaggtctca tagcccattg gcattttaag tatatcgtca tgtatttgtg ccatttttgc
      841 acattgttca atgagctcca tatttgggct ttcatcaaaa aatgaaatat tttccccaat
      901 agaacctgca aaaaggacat catcttggcg aacaatcccg ctcaatcccc taataaagga
      961 tggagaaact tgatatatat catgcccatt aatactaact gtaccagttt caggttttag
     1021 gctacctgtt aaaatgttta acaaagtgga cttcccccga ccagattgtc ctgttaaaac
     1081 aactgcttca ttatctttaa attccaaatt aatgttttca aaaagatatg gctcattatc
     1141 agcatatctg aatgagacgt tttcaacttt aagaaccagt tgttcattat ctaatttagg
     1201 tatatgatta tacttaataa tttcagtttc tgtttcattt aaagtaatgt cagccaaacg
     1261 ttcagcatga agccctaaca ttttgatttg gatgtattgg tcaacgagag aagctgttct
     1321 gctttcaaat tgccctttat aagccaaaaa agccatcaga acaccgactg taaatgaacc
     1381 atctaaaatt gcgcttgcac caagataaat tatgataata ttttccatgc taaacaacag
     1441 tttatttgaa aattcaaata aagcagagag tttatctgtt gtcagcttgg tattgactgt
     1501 attcacaaat aggctcatcc aagtgccatg tctttgataa tgtttatcaa ataatttaac
     1561 tgattggata ccacgaatgg tttccatgaa atatgagttt tgtttggctt catgaacaat
     1621 attttcttct gttgcatttc ttaatgggta atatgcaagc caacgaatta gtatgtacaa
     1681 aacaagtgtt aaaagaacaa tcagcgataa ttgagtgctg taaattgtca ttaacacgaa
     1741 agtaaaaaca gccattaagc tatttaaaac taaaacaaaa aaagtagaag ttagtgtttc
     1801 ttggatatga tctattgaac caaatcttga aatcacatct cctaaatgtc gtttactgaa
     1861 atagtcatta ggtaagtcaa gtaacctttt aaatatattg gctttccatt gtaaattaag
     1921 agttgtagat aggtgcatac ctacccatgc ttgtaacagg ctaattaact gttgcaggat
     1981 agccagtaaa ccaaatccca aagtaagggt cagtaataaa tttttatcag cagttacaat
     2041 gacatggtct attacccatt gcataaagaa tggactaacc aatgcaaaga cttccaaaga
     2101 aatagctaat ataagcattt gaattaaaga acgttttaag cctgaaatcc cccttaatag
     2161 agataatatt ttgattttct ttgtttcttt tttctcttca aaatgggtat tggggaataa
     2221 ttctagggca atccctgtga atttttgtga aacttcgtcc attttgattt ttcgcatacc
     2281 gacagcaggg tccataatga cgatactgtc tttggaaatg gaacaaagta caacaaaatg
     2341 gtttaagttc caatggagaa tgcagggtag ttgtaaattt gacagctcat ctaactctaa
     2401 acgcaaagct cgtggcgtta aattcatttc attgccaaat ctcatgatgt ctgcaagatt
     2461 tgcgcccttt aatgacaggg tgtatttttg gcgtaacgtt cttaaatcag tgtgaaagcc
     2521 ataataggac aagatggatg tcaggcatgc taaaccacat tcagcaactt ctgtttgcag
     2581 aatgacaggt agctttttgt taaatccaaa ggacagtctt gataaataat ccatttttaa
     2641 ttgatttttc ctgaaatgct gtaaagtggg tcaagtaccc attcgtacaa tttttttcgt
     2701 tcatggagaa tatctgcttc taaaatcatg ccaatttgaa gcggcttgtt ttctccgtat
     2761 gctttaatcg tttgtttttc caatttaact ttcacaagat aggcaggttc atttaataag
     2821 gttgggttag tgaaaatgat acctaaacct gatagctctt gtttaccgag agcagttctg
     2881 gcaactgaaa taatttctcc tgtggcatgt ccaaattttt ggtaagggta cgcttggtaa
     2941 cgtaaaacaa ctttatcttt cggtttaata aaaccaacag ctttactggg tatgtaaaga
     3001 ttggcgacca attcagtttg ttcagggaca atgcttaaca gcaatttaga cggttcaact
     3061 tgttgcccta tatcaacatt aattgttgaa atataacctg atttactagc tcgtatggtt
     3121 tgttcggatt tcaaatcaaa atccaaaatt tcttggttca tttccgtaat cgcacggttg
     3181 agttggctca attcggtttt atggcgttca ggcaggctgc ttaatgtgat tttctgttca
     3241 tcaagttccc tgattgcatt attttgttca cgttttaggc tctccaaacg tgagcgttgt
     3301 tccaataaat ggctttcggc ggtcatctta tcttgttggg atactgcgcc ttgactggct
     3361 aaaaacttgt tcttgttaag ggttttttcc actaaacgaa tttgacgatt ttgccctgta
     3421 atttgctgtt taatattctc taattgattg tttaaacgat gaatgttgtt atgaacattt
     3481 ttttgctcat tttgatgaat gcgctttaaa cgttccaatt cttgtaatgc caaagttttt
     3541 ttaaggttgg cttctgctgc caatttggct tgtacgtttc ctttttcgcc aaaacgcgat
     3601 gtggaaagtt tgaacaatgg ttcgccagct ttgacaaagt taccattttc aacaaattta
     3661 tgcgtaatcg tgccgatatc ggaagagtaa acacgaacca cccccatagt tggaagtaat
     3721 tgaccttcaa cggttgtttt attggtatag ctaccaaaaa tcaaaaagat aatgatacac
     3781 agagcaatga gaaaagcgca aaaagtcaga aataaaaaag agaatggacg ggtcaagata
     3841 acctgacctg tccacttatt ttgttgggct acaaagactt cttttcggaa taagtgggac
     3901 atttattatt atttcttaaa aaaaatggat agaaaaacac caataattaa atacttatgc
     3961 ctaataacat gaatttgcta ttgattgatg tttctatttt aattacatta aatttaacca
     4021 ataatccaga aatggttgca gctaaaatgc aaaagacagt ggcgtaaatg gtttcagtat
     4081 tcataatttt tctctttcaa aattgttcaa tctaaataga aaaaggtaac ttcaatacat
     4141 attgctgtaa ctgaaattac cttttcctta atgatgtaat tatcttatgg cttcatcatg
     4201 ccacgaccga tattgtatcc gccagcagca gagaaaatac cagctccaat tgcgccaacc
     4261 cagcctactg gtcctgtcca cttgttccgt tgagctacaa agacttctgg tctgaaaaat
     4321 gagtgattgt tagacatgtg tacttattta tgtttctttg attgggataa atttgtaaaa
     4381 acaaaaaaaa gcaagttgtt attatagaag aacttaaaat gcttaacata ctcattgagg
     4441 tttgtaccac ataatgagat acgagataat ttgttgggag taaaatcaaa aaaaagatta
     4501 aatatttaaa ataatcacta tattttttca ttatttgttc ctttttgtat ttaagttctt
     4561 atgaaaaact agggtatttt gaatagaaaa tatcaaaata ccctagtttt attaaggaat
     4621 attaccacca acaagtgctt ccatattgta cagcaccagt gattgcacca ccaatagctc
     4681 cgaatttcgc acctggaata gcaccaatac cacctgcaaa tgaaccaaca attgccccac
     4741 cagctgctcc acctaatgca ctaccaatgg tattttttga gaaatcacgc cagttacaag
     4801 cagcaccaga aacttgttaa atttcattca aagtcaaaac ttgcataata ttcacctttg
     4861 ttaagtgtgg ttgagaaaaa agtaaatttt catcgttact cattatttgc aatcatgcat
     4921 atactgtaac caagcactat attcagttgg atcatagttt ttcctactgg gttgtctgat
     4981 atgactattg gcactattgg cacaattggg attagcaact ttattgtgag tacctaatgg
     5041 attgatattt cctgtaaagt ggctattgca tgcgcttaat gagcatagat ttattaatgt
     5101 cagaaaggat aaaacaaaaa atcttaccat tttttactaa taccaaaagt aggtttcgaa
     5161 aactgtacac caagaccttg tacatatcca aattgcttgc taacagaaaa ctctactcca
     5221 ctattgttag gggaatagga agcaccaatt ttaccaacag caaccatatc ttttttacct
     5281 aggttgccgc caccctgtgc agagatatgg aatttgccac ctgacacttc aactaattca
     5341 gaggtgtgta actctttcat gattttctcc tgtaaagaag tatggttgga aatgtaaatc
     5401 agaattaacc tttcttaagt ttaatttaat actttttttt gcaagtattt tattttaaat
     5461 taaatcaata ttttaaaaat atttacttgt atactgaaaa agaaactcct aatggtggag
     5521 tggggctttt gtttaagtaa gcattgaaaa aatttaaatt tcccattatg attaacttaa
     5581 tgcaaaagct ttttcaggac gtacaaacca gcgaacgggc gttctgatgt gggcaggttg
     5641 gttgtcgcta accaatttca gtagctcaat cgtcagattg gtatccgacc ctatcaagct
     5701 aaggagaggc tgcggctact acacaaagca cctgaattat tgggtgcttt tgtcttttta
     5761 tggcattgta ccaatttgtg taccacgagg gcttgttcgc ccaaacgcgg taacaactga
     5821 atacacactt tccccctcaa cttctttgcg ttctaaaacg actgtcccaa tccgagaata
     5881 gacaatcata ggacgatgat tgccccttac atcactaaat tcactaaaaa tttttgcgcc
     5941 tgattgaaaa atcatttcca cataaaaggg ttacacctgt ttcatcgtgg cttaatccaa
     6001 accgcgttaa atctgctttg tgttctgcca aaatgtgaat cacaccaaag ccgtgattaa
     6061 cctttttgtg ttcgccgtgt tttaaaacaa tttgacctgc cgggaagtct gctctattgt
     6121 tcggacaata gggggaatat accaattact ttaactacat cattttttga catttttctt
     6181 gcctttcaat ttaaaatcca aaatcatact gccataattt agcaatccaa aaaaatttag
     6241 gcagcagtaa tggtttcgta ttttagaaaa cgaaacttgg tttggtgttt tgaagcattt
     6301 cagtacacaa acatggaatt tt
//
LOCUS       DAXHSJ010000120         6146 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000120 DAXHSJ010000000
VERSION     DAXHSJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.120"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(275..709)
                     /locus_tag="ACFM8U_001600"
     CDS             complement(275..709)
                     /locus_tag="ACFM8U_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC6334814.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(712..1374)
                     /gene="exbB"
                     /locus_tag="ACFM8U_001601"
     CDS             complement(712..1374)
                     /gene="exbB"
                     /locus_tag="ACFM8U_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC6334815.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1440..2282)
                     /locus_tag="ACFM8U_001602"
     CDS             complement(1440..2282)
                     /locus_tag="ACFM8U_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC6334816.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2537..3175
                     /locus_tag="ACFM8U_001603"
     CDS             2537..3175
                     /locus_tag="ACFM8U_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC6334817.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3627..4427)
                     /locus_tag="ACFM8U_001604"
     CDS             complement(3627..4427)
                     /locus_tag="ACFM8U_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFC6334818.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4563..5069)
                     /locus_tag="ACFM8U_001605"
     CDS             complement(4563..5069)
                     /locus_tag="ACFM8U_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334819.1"
                     /translation="MDFFDFFAQGKYKKIATEQGLDRKYFIFHYIVCNLSAISSFCLM
                     KVIHSYTILCLLIMIIGIAFGFILRNRVLSIVKNKQNFLSDMFPLEMPFFSRVYKIPI
                     LARFLNRGMKWLYLQNFFCLVMICFSISLILNEERGWMPYFICYWFGLCYPANTYYVF
                     LSRDYKHI"
     gene            complement(5406..6047)
                     /locus_tag="ACFM8U_001606"
     CDS             complement(5406..6047)
                     /locus_tag="ACFM8U_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC6334820.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
BASE COUNT         1641 a         1540 c         1270 g         1695 t
ORIGIN      
        1 cggcttcgtt gccttgtcct gatttttgtt aatccactat aaaaattaga aatgcacatt
       61 ttcattattc tcgcgcaggc aggactccag acttacccat ttcagtaatg tttgaaaata
      121 aaagaaaaat cagatgtttg tattcccgcc tgcgcagaaa tggagacggc gctctgtcgt
      181 ctcatttttg ttttaatcaa ctatatatag ctgattaaac ataagaaatg ccgtctgaaa
      241 gactttcaga cggcattcgt tcaagcgtcg aactttattg cgccttggtt tcggttacaa
      301 aaccgatttt ggtgattcct gcctgacggg cggcttctaa agctttgttt acataatcgt
      361 attccaccgc cttgtctgcc gcaatcgcca caatcacgtt ttcattctgc tccttggcgg
      421 ctttcagacg gctttccact tccccgattt ccactttgct tgcagaatcc ccgccgacat
      481 aatagccgcc gttcgcatca atcgtcaggc gcagggggtc tttaggctgt ttgtcctgct
      541 tgtttgtctg ctcggacgcg gtcggcagtt ccaaagggat ggaatgcgtc agcaccggca
      601 tagtaatcat aaacacaatc agcaacacca gcatcacgtc caccaacggc gtaacgttga
      661 tgtcggacat cggagaatcg tcgccggaat tcatcgaacc aaatgccata atcagctatc
      721 cttttgatta agcaggcgga cgtgcaaatc gtgcgccatc gcatccaaat cctgggtcag
      781 tatttttgtg ccgcgattga ggaagttgta tgccaacacc gccggaatcg ccacgaacaa
      841 acccgccgcc gtcgccacca gtgcctcgcc aatcgggccg gcaaccgccg caatactcat
      901 ctgcccgctt tgcccgatat tgatcagggc gtggtaaatc ccccaaaccg tgccgaacag
      961 cccgataaac ggcgcggtcg cgccgatgga ggcaagcgcg gtcatcccgt aatcaaaccg
     1021 gcgcataatc tgcgccatac tgttgcggat ttgaatgacc aaatactcgt tcaacggcaa
     1081 agcctgcgcc agttcggacg cttcgtttcg gcggtagttg cggtaagact gcaatgcctc
     1141 ttgcgccagt ttggacaaag gcgcatcgac ggcgcgcact ttttcgaccg cgtcgttcag
     1201 cgacaaagta tcgcgcatat gccgtttgac ggcggcattc cctttgcgcg cccgatacag
     1261 cttgatgcag cgcaagacaa ccaaacacca cgttacgata ctcatcaaca gcatcaacac
     1321 aaacacaccg atcaggacgg gatcgcccga ttcaaatact aatttcaaat tcataatgat
     1381 tccaacactg aaaaaaccaa tcaaacatcc aagctgccgc aaaccgctgc ggcaaccgcc
     1441 taattcaatt caaacttgac ggggacttta aactccgtcc aggcattggc ttgaaaatgc
     1501 ccgttttgcg ccgccttgcg tgccgcattg tccaaccgag aaaaaccact gcttttcacg
     1561 attttaacgg actcgacatg accgcccgga gaaaccaaaa cgctcaaaac aaccgtaccc
     1621 tgctcgtcat tctccataga aagcgtggga taagccgggc gcggaatgct gccgttggcg
     1681 cgtaaaggat tgcctttgct gctgccggct ccttccccgt gttcgccttt gacaccgccg
     1741 ctacctttac cgctgccttc tccgcgcccc gttccgtctc ctttggtacc ggttccctta
     1801 tcttccccat taccctgctc gctgtctgct ttggcagaag cattgctggg atgttcggca
     1861 ggtttttcag acggcttctc gaccggtttt tccgccggtt tcgggacagg cttcgcttcc
     1921 ggcttaggct ctggtttcgg tttttcttcg ggtttcggct tttcttcagg tttcggctct
     1981 tccttaggct gctgaatatc cgcatccgcc tttttcgtaa ccaccggctt caaaaccggc
     2041 ttgggcggct cgacaggttt gggcggctca ggctcaggtt gcggctcagg cgcagcaggc
     2101 gcacctgcgc cttcgggtgc accgtcccct ccgccaaaat cacccaaatc gacaaattcg
     2161 ataacatttc ctgactctat cacgggcagc ttgtgcgcct gccagagcaa tgccaccatt
     2221 gccaaatgca gcagtgcgac ggaaaacacg actgcggggg ttaaaattcg ttctttatcc
     2281 ataattcggg cataataata gcaacaattc ctatttgcaa cctattttta caatttttgg
     2341 tcatatgaat gtctgttccg ttcacaggca aaccgtgttt aaacgctgta ttacagcaaa
     2401 tcatcagata acgggccggc agaaaaaatg attccgtctg atttcttatt ccaataaaat
     2461 caggttagat gatatattgc cgcttctgtc tgtcagccgt ttcgggctgc acaccacatc
     2521 tgttcaaagg aaaaccatgt ttcaaaattt tgatttgggc gtgtttctgc ttgccgtcct
     2581 gcccgtgctg ctcgccatta ccgtcaggga ggtggcgcgc ggctatacgg cgcgctactg
     2641 gggagacaac actgccgaac aatacggcag gctgacactg aaccccctac cccatatcga
     2701 tttggtcggc acaatcatcg taccgctgct tactttgatg ttcacgccct tcctgttcgg
     2761 ctgggcgcgt ccgattccta tcgattcgcg caacttccgc aacccgcgcc ttgcctggcg
     2821 ttgcgttgcc gcgtccggcc cgctgtcgaa tctggcgatg gctgttctgt ggggcgtggt
     2881 tttggtgctg actccgtatg tcggcggggc gtatcagatg ccgttggctc aaatggcaaa
     2941 ctacggtatt ctgatcaatg cgattctgtt cgcgctcaac atcatcccca tcctgccttg
     3001 ggacggcggc attttcatcg acaccttcct gtcggcgaaa tattcgcaag cgttccgcaa
     3061 aatcgaacct tatgggacgt ggattatcct actgctgatg ctgaccgggg ttttgggtgc
     3121 gtttattgca ccgattgtgc ggctggtgat tgcgtttgtg cagatgttcg tctgactggc
     3181 tttcagacgg cataaacgct ccagaaaacg cggcaggaca tattgccctg ccgcgttttc
     3241 ctgtagtgta atcttatttt tttcatcatt attagaacca ggttgcatga taataccttt
     3301 cattaactga aacactgatt aagaaactcc agtctgtcta atgatgaggt tttcacatcg
     3361 ccaaaacttg ccaatcaaat gctggattta ttgccgtctg agatttggtc aaatccaaag
     3421 gcgacattct tagaccctgt gtgtaaatca ggggtatttt tgcgtgaaat cgtcaaacgc
     3481 ttggatgaag gcttgaccaa tcaaatacca gataaacaaa ctcgcattaa ccacatttta
     3541 aaaaatcaag tttttggaag tactgccacg tatgtaggta gctttgaccg atatttgcat
     3601 aaaaactcct ttgctggtga aaggaattat tttgccaatt ttaaaatatt tctggcacca
     3661 aatagtacaa tgacaaagac aatcatgcca atgattaaat caggatagct agaatgagtc
     3721 aataacgtca atgctcccgc cgctatcaca ccgatattga tgataatgtc attggatgta
     3781 aaaatcatgc tggctttgat atggatttct ttattttgat ttttgctcag tagatataag
     3841 cacagccagt ttgcaatcaa tgccaaaaat gccgtgccaa tcatcagttg ataattgggc
     3901 agctgctcag caccgataaa acgcctaatc acttctatca ccccaaataa cgccaatatt
     3961 atctgcgtta tccccgccaa aaatgccaca cgttttttat acgccagcgt cataccaatg
     4021 gctgatagcg ccaatatata gacaaagctg tccgccagca tatctagact atcagcaatc
     4081 agccccatag aattagcaaa aataccaacc gaacactcta tgataaaaaa cacaaagtta
     4141 atcatgagca cttgatataa taatcttttt tctaagtgct catcaggctt gttaaacact
     4201 atcttatcaa caatcacttc ggtggaaatg atatgactat caaaattaag cggttcaagt
     4261 acttgtaaaa tcgttgtatc ttgattatcg tgatagacgg ttaagcaccg cccagcaata
     4321 tcaaactgta attcataaat atcagacaca tcttttaaac gcatgcgaat gagctgttct
     4381 tcggacgggc agtccatttt ggtaatgtta aaaatggtct ttttcatcta tttagttcct
     4441 tgttttgatc aggttggctc aaataaatct gtgtttatat tgctgcttgg taatttttgg
     4501 atggtttgag taaattgatt aggttaaaat ttacctttgg aagtaccgcc acgcataata
     4561 gtttagatat gtttataatc tctggataaa aaaacgtaat aagtgtttgc tggataacaa
     4621 agtccaaacc aatagcagat aaaataaggc atccaccccc tttcttcatt aaggataaga
     4681 gatatcgaga aacaaatcat aactaaacag aaaaaatttt ggagataaag ccatttcatt
     4741 cccctattca agaatctggc caagataggt attttgtata ctctagaaaa gaaaggcatt
     4801 tccaagggaa acatatcaga taaaaaattt tgtttatttt ttactataga tagaacccta
     4861 tttctcaaga taaatccaaa tgcaatgcca attatcatta ttaataaaca aaggatagta
     4921 taactatgga taaccttcat taaacaaaaa gaagaaatgg cagataaatt acatacgata
     4981 tagtggaata taaaatattt acggtctaaa ccttgttcag ttgcaatttt tttatactta
     5041 ccttgcgcaa aaaaatcaaa gaaatccatt aaactatctt tcacattaga aatttaaaag
     5101 ctaaataata cgacaaacaa tgtgaagtac tattcatggt ttatttaaaa aataatacta
     5161 ttctgaacat tatttagata cagaaattaa caaattagaa ctaaacaagc ttttaaatac
     5221 tttaatttta ttggaaagct ataaaaggaa ctataacttt acacactagt cacttctttt
     5281 taagaggcaa aagggattgg gaaggtcgtc ttggagataa gcactggtat ttcggccaat
     5341 ggtaaataga gtttacctca aatagggtag aacctccttc atctgtcagt taataacagc
     5401 cacttttaca atgccctgtc gaaataaagt ggcacgcccg atttttcact cataccgatt
     5461 aaataatcca tcgtcttccg agggaacgcg atgccgcgca ccacggtcag attcctcaaa
     5521 acggggaaaa ccaaaatatc ctccataccg attccgccgt tgatgccgtc tgaagcaccg
     5581 tccatcaaat tttccaactc ttgcaaatct gcgtttatcc gttcgaggta ttgggcggtt
     5641 ttattcaaat tggcggaaaa gctgccgatg cttttctctt ttttgtctgt aaaatatttc
     5701 accgcttccg gcgttgcaaa ttcaggcagc ccgattttga tcacgcgcgg ctgcaccagt
     5761 ttgtcgttgt atccgcccac cttgtccagc cacgcccgta tctcggggcg gacttcgtct
     5821 ttcagacggt cttcgcggtc gaaatgccgc acaatgtcca aactctcgcc cataaacgaa
     5881 ccgtcttctt tttgcaggac gggcacttgt ttcgcaccga tcataccgat cggcgttgcc
     5941 tcgtcgtcgt ttgccagcac ggcttcttca acgtccgcgc caaacagccc ggcagccatc
     6001 cgcgcacgca cgcaaaacgg gcaatggtcg taaatataca gtttcatcaa aatattcctc
     6061 gtcaacctgt cggtaccggc taccttaaca ccccgcgcca cccgaaacaa gtttatcttc
     6121 ccgcctatgc accgtaaata aataag
//
LOCUS       DAXHSJ010000121         6028 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000121 DAXHSJ010000000
VERSION     DAXHSJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.121"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            164..1276
                     /gene="nadA"
                     /locus_tag="ACFM8U_001607"
     CDS             164..1276
                     /gene="nadA"
                     /locus_tag="ACFM8U_001607"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC6334821.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            1328..2836
                     /gene="nadB"
                     /locus_tag="ACFM8U_001608"
     CDS             1328..2836
                     /gene="nadB"
                     /locus_tag="ACFM8U_001608"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC6334822.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEASAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NYDGLHLTREGGHTCRRIAHVADYTGEAVMQSLLAQIRRRPHIRVCERQMALDIQTES
                     GVACGLIVLDRRTQETYLIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTPDFVSLDISRQPEAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSENPSAEAGIFADDLQNTFSRPVLQTFNQRHLGILRSDTGLRR
                     AIAQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(3012..3677)
                     /gene="pgmB"
                     /locus_tag="ACFM8U_001609"
     CDS             complement(3012..3677)
                     /gene="pgmB"
                     /locus_tag="ACFM8U_001609"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFC6334823.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(3689..5947)
                     /locus_tag="ACFM8U_001610"
     CDS             complement(3689..5947)
                     /locus_tag="ACFM8U_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFC6334824.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFDVCKFLSVAQKELALIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGRQTDWQVSNSFEAEVGGTPETFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKIAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRRGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNMDEKRRNFDFYEPMTVHESSLSPC
                     IHAILAAELGKEEKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGTLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLELQVYGKDI
                     TLNGSHTVTLEK"
BASE COUNT         1253 a         1688 c         1756 g         1331 t
ORIGIN      
        1 caaagcggtt tgtcggaacg aagcgggtcg gttttacggt atcgcaaacc tgttttcaga
       61 cgaccttgct tattatactc aatttgagca taatataaaa atatttcaaa atataaacgg
      121 caaatcttta tgccgtctga aaaaccagcc ataaggaagt ctcatgcaaa ccgccgcccg
      181 ccgctcgttc gattacgata tgcccctcat ccaaaccccg acttccgcct gccaaatccg
      241 tcaggcgtgg gcgaaggttg ccgacacgcc cgaccgcgag acggcagatc gtctgaaaga
      301 cgaaatcaag gctttgctga aggagaaaaa cgcggtcttg gtggcgcatt attatgttga
      361 tccgctgatt caggatttgg ctttggaaac gggcggatgc gtgggcgatt ctctggaaat
      421 ggcacgcttc ggcgcggaac acgaagccgg tacgctggtg gtggcgggcg tgcgtttcat
      481 gggagagagc gcgaaaatcc tctgccctga aaaaacggtg ctgatgcctg atttggaagc
      541 ggaatgttct ttggatttgg gttgcccgga agaggcgttt tcggcgtttt gcgaccaaca
      601 ccccgaccgc acggtggtgg tgtacgccaa cacttccgcc gccgtgaaag cgcgtgccga
      661 ttgggtggta acgtcttcgg tggcgttgga aatcgtgtcg tatctgaaat cgcgcggcga
      721 gaagctgatt tgggggcccg accgccacct cggcgactac atccgccgcg aaacgggcgc
      781 ggatatgctg ttgtggcagg gttcgtgcat cgtccacaac gaatttaaag ggcaggagct
      841 ggcggcgttg aaggcggaac accccgatgc ggtggtgctg gttcatcccg aatcgccaca
      901 aagcgtcatc gaactgggcg acgtggtcgg ctcgaccagc aaactgctga aagctgccgt
      961 atcacgcccc gaaaaaaaat tcatcgtggc gaccgatttg ggcatcctgc acgaaatgca
     1021 aaagcaggcg cccgacaaag aatttatcgc cgcgccgacg gcgggcaacg gcggaagttg
     1081 caaaagctgc gcgttctgcc cgtggatggc gatgaattcg ctgggcggca tcaaatacgc
     1141 cctgacaagc ggacgtaacg aaatcctgtt ggacaggaag ctgggcgaag ccgccaaact
     1201 gcctttgcag cgtatgctcg acttcgcggc aggactcaaa cgcggggatg tgttcaacgg
     1261 catggggccc gcctgatttg ccgtccatat tgccgtttca gacgacctct ccaacaagga
     1321 caacaccatg caaaccgatt gcgacgtatt gattgccgga aacgggctgg cggcactgac
     1381 gctcgccctg tcgctgcctg aatcgttccg catcgtcatt ttgtgcaaaa accggctgga
     1441 cgacaccgcc agccgtcatg cgcaaggcgg gattgcggcg gcgtggtcgg gagaggacga
     1501 catcgaaaaa cacgtcgccg atactttgga agcgagcgca ggtttgtgcg acgaagccgc
     1561 cgtccgcacc atcctgtcgc agggcaaacc ggcaatcgaa tggctgctgg cgcagggcgt
     1621 ggcgttcgac cgaaattatg acggcctgca cctgacgcgc gaaggcgggc acacctgccg
     1681 ccgaatcgcc cacgtcgccg actacacggg cgaagccgtc atgcagagcc tgcttgccca
     1741 aatacgccgc cgcccgcaca tccgcgtttg cgagcggcag atggcgttgg acattcaaac
     1801 cgaatcaggt gtggcgtgcg gactgatcgt ccttgaccgc cgaacgcaag aaacctacct
     1861 catccgcgcc cgccataccg tactcgcagg cggcggcttg ggacagattt acgccgccac
     1921 caccacgccg cccgaatgca cgggcgacgc catcgccatg gcgatacgcg caggctgtgc
     1981 agttgagaac ctcgaattta tccaattcca ccccacaggc ttggcaagat cgtctgaaaa
     2041 cggccgtacc ttcctgattt ccgaagccgt gcgcggcgaa ggcggcatcc tgaccaacca
     2101 agcgggcgaa cggtttatgc cgcattacga ccgccgcgcc gaactcgcgc cgcgcgacat
     2161 cgttgcccgc gccatcgccg ccgaaatcgc caaacaaact ccagacttcg tctcgctcga
     2221 catcagccgc caacccgaag cgttcgtccg ccggcatttt ccgtccatcc atcggcactg
     2281 tttatcccaa tgcggcctgg acatcacgcg ccaagccatc cccgtccgcc ccgtgcaaca
     2341 ctacacctgc ggcggcatcc aaaccgaccc ctgcggcaga acctccctgc cgcagctcta
     2401 cgccttaggc gaaaccgcct gcaccggctt gcacggagcc aaccgcctcg ccagcaactc
     2461 cctgctcgaa tgtgtcgtta ccgccagact tgccgcccaa accatcgcgg acggacaagc
     2521 attccaagcc aaaacgtctc caaggccgtc tgaaaacccc tccgccgaag caggcatctt
     2581 tgcagacgac cttcaaaaca cattcagccg ccccgtcctg caaacgttca accaacgcca
     2641 tctcggcatc ctacgcagcg ataccggcct gcgccgcgcc atcgcccaac tgcggctttg
     2701 gaagcaaaac caagccgaac cgcacaccgc gtccgaatac gaaaaccgca acctgctcga
     2761 atgcagcctc gccgtcgccc aagccgcata caggcggaga cagaacatcg gcgcgcattt
     2821 taatagtgat tgttaaggtt ggctggttag aaaaaatcgt tcaaatgaat tttaatagca
     2881 tggttaagag attggcattc agtttttagg gttgcgtaag ttgggttatc aatctgttct
     2941 aaaagcagaa aggccgtctg aaacactgtt cagacggcct tttacttcct tgacacttta
     3001 tctgacgcgt tttacctgcc cgactgtgcc cacacgtttt gcaggtaggc gtaggtcagc
     3061 tcggcggtat cggacaccag cgcgatgtcg ctgcccaagt cttcggcttt gcccacgccg
     3121 ataggcaagg cgccggcggc tttgatggcg gcgacacctg cggcagcgtc ttcaatgccg
     3181 atgcagcggc ggatgtccgc gcccacgccc tcggcggcgg cgaggaagat gtcgggggcg
     3241 ggtttggaat gcgcgacggc ggcagggtcg gcgacggtgt cgaagaagtg ggtcagcccc
     3301 atgcgttcga gcaggaaggg gccgttttta ctggcggacg caagggcgat ttttttgccg
     3361 tttgctttta atgcttccag cagcggcaaa atgccgagat acacgtcttc gggtttgact
     3421 gcctgaatca tctcgacgta gttgtcgttt ttgcggcggg tcagttcggc gaactcggct
     3481 tcgccgacgg ttttgccgcc gtgtgcgagg atgcgtttga gcgaatcgtc gcgcgacacg
     3541 cctttgagct gctcgttaaa cttgcggtct atgccgatgc ccagttcttc ggcgagcttt
     3601 ttccatgcgc ggtagtggta ttcggcggtg tcggtgatga cgccgtcgag atcgaagagc
     3661 actgcggtaa acgtcatttt gcgccccctt atttttccaa cgtgacggta tggctgccgt
     3721 tgagcgtgat gtctttgccg tacacctgca attcgagcgg ttcgcctttg agcagggtga
     3781 agacgacgtt ttctttgccg acggcgactt taatcagacg gcctcggtag ttgatgtgga
     3841 aggcgtagcc tgtccacgca ctcggcagga acggtgcgaa gctgagtgtg ccgccccaag
     3901 ttttcatttg ggcgaaacct tggacgatgg cgagccacga gccggtcatg gaggtaatgt
     3961 gcaggccgtc ttcggtgtcg ttattgtagt tgtccaagtc cagacgggcg gtgcgctggt
     4021 acatttccac ggctttttct tctttgccca gttcggcggc aaggatggca tgaatacacg
     4081 gcgacaacga gctttcatgc acggtcatcg gttcgtagaa gtcgaagttg cgacgttttt
     4141 cgtccatatt gaaacggtcg ccgaagaagt agatgccttg caagacgtcc gcctgtttga
     4201 tgaagggcga acgcaggatt ttgtcccacg accatttctg gttgagcggc aaatcgtcgg
     4261 gcgaaagcgc ggacactggg cggatgtctt tatcaaggaa gccgtcgtgt tggacgaata
     4321 cgccgagttc ttcgtcatga ggacggtaca tattcgcgct gatgtccgcc catttttcca
     4381 actcggcggc acgcacgttc aaatccggac gcgggtattt cgccaaggct tcgcgggtgt
     4441 agtccaatac ccaagcggca agggtgttgg tgtaccagtt gttgttgatg ttgttttcgt
     4501 attcgttcgg gccggttacg ccgtgaatca tgtatttacc cctgcgtttg gagaagtgga
     4561 cgcggtccgc ccagaagcgg gacacttcga ccaagacttc caagccttct ttggcaagat
     4621 agctttcgtc gccggtgtag ttggtgtagt tgtagatggc gtaaggaatc gcgccgttgc
     4681 ggtggatttc ctcgaaggtg atttcccatt cgttgtggca ctcgatgccc gtaaacgtta
     4741 ccatcggata gagtgcgccc gccaagccct gttcgcgcgc gttgtgctgc gcctgcggca
     4801 gttggttgcg gcggtattgc agcaggttgc gggtaacttc gggttccgcc agtgcgaggt
     4861 aaagcggcac ggcgtaggct tcggtgtccc aataggtcgc gccgccgtat ttttcgccgg
     4921 taaagccttt cgggccgatg ttcaggcgcg cgtcttcgcc gtagtaggtg gagaacagtt
     4981 ggaacaggtt gaagcggatg ccctgctgcg cttcgtcgct gccttcgatg accacgtcgg
     5041 cgatttccca acggtgcagc cagcctgctt tgtgcgcgtc cagcaaggtt tcaaacgcaa
     5101 cgcctgcgat tttttccgac aaggcgcggc ctgcggcttt cactgcttcc aagccctgat
     5161 aatcgcggct ggtggtaaca atcacgcgtt tttcaaaggt ttcgggcgtg ccaccgactt
     5221 cggcttcaaa agaattggag acctgccagt cggtttgtct gccgccgagg gctttaaagc
     5281 tgccggcgaa ggtttgctcg gcgttgacga taaattgttc cacgccgaaa gggttggcaa
     5341 cggtttgggc ggcaatgtag gagagactgt ctgaaacgcc tttgtccaat acctgccaga
     5401 atttttcttc gtagttggag tcttcgtttt tcacgtcggc atcgatgatg gaatcgatgc
     5461 ggacttggtg ggttttgccg tcaacggata cggcttccca gcggatgaga gccagctctt
     5521 tttgtgcgac ggacaggaat ttgcacacat cgaaacgcac gccgaatacg gtgaacgagc
     5581 ggcgcaacac gccgtgctgc atatcgagtt cgacggagaa gccggcaaca tcgtttttcg
     5641 ccaagtccac ctcttgacca tcgacaaaga ttttgacttt gctgaaattg agcgcgttga
     5701 tggctttgcc gaaatatttg gggtagccgt ttttccacca gccgacgcgg gttttgtcgg
     5761 gaaaccacac accggcgatg taggtaccca agtggctgtc ggcggaatag gtttcctcaa
     5821 agctgccgcg catacccata tagccgttgc ccaaactggt caggctctct tgcagccgtt
     5881 tgtgttcttt ttccagcttt gccgaacgca gcgtccaagg gctgatttcc ataattcttg
     5941 tgtacattta tgaagctcct gtttggattg atttgaggga atggtgaaat cttatagtgg
     6001 attaacaaaa atcaggacaa ggcgacga
//
LOCUS       DAXHSJ010000122         5979 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000122 DAXHSJ010000000
VERSION     DAXHSJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.122"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(45..968)
                     /gene="truB"
                     /locus_tag="ACFM8U_001611"
     CDS             complement(45..968)
                     /gene="truB"
                     /locus_tag="ACFM8U_001611"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC6334825.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIAAARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDI
                     AEFDSPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLDETERDSLLLPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFEENLPFNTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(1001..1372)
                     /gene="rbfA"
                     /locus_tag="ACFM8U_001612"
     CDS             complement(1001..1372)
                     /gene="rbfA"
                     /locus_tag="ACFM8U_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC6334826.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            1563..2807
                     /gene="clpX"
                     /locus_tag="ACFM8U_001613"
     CDS             1563..2807
                     /gene="clpX"
                     /locus_tag="ACFM8U_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC6334827.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIAWQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGKEPELVFES"
     gene            complement(2872..4065)
                     /locus_tag="ACFM8U_001614"
     CDS             complement(2872..4065)
                     /locus_tag="ACFM8U_001614"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC6334828.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            4469..4969
                     /locus_tag="ACFM8U_001615"
     CDS             4469..4969
                     /locus_tag="ACFM8U_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334829.1"
                     /translation="MNAMPQTAAQNETLHICSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            5054..5608
                     /locus_tag="ACFM8U_001616"
     CDS             5054..5608
                     /locus_tag="ACFM8U_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334830.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            5622..5822
                     /locus_tag="ACFM8U_001617"
     CDS             5622..5822
                     /locus_tag="ACFM8U_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334831.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
BASE COUNT         1412 a         1602 c         1625 g         1340 t
ORIGIN      
        1 tatcaacaca ttattcaaac acagcccgta tttttaaccg ccgttcaggc ggcggatgcc
       61 gccgtgttca tcaggcgcaa ggcttttaga cggcatattt ccttttgata ttccgccagg
      121 ccgacaaagc ggccgttttc cgtgtaaacg cgtaccggcg tgttgaaagg caggttttcc
      181 tcgaaacgcg gtcgttgccc gcattggagc atatggacgg cataatcgtt taaaacggtt
      241 tggggaaaat gggcaaccaa tacgtcgcag ggcagcagca ggctgtcgcg ttctgtttcg
      301 tccaagtttg ccaaggcttc gagcgtgtgg ctttgggcga tggtaaagcc ggcggtttcg
      361 gtgcggcgca gggcggtcag gtgggcgaac gtgccgatgt gtttggcgat gtcttcgctg
      421 agggtgcgga tataggtgcc tttgctgcaa cgcacgtcga tgacggcttt gggtgagtca
      481 aactcggcaa tatcgatggc gtaaacggta atgtcgcgcg ccttgcgttc gatgacgatg
      541 cctttgcggg cgtattcgta cagcggtttg ccttcgtgct tgagggcgga aaacatcggc
      601 ggcacttggc ggatgttgcc tgtcagggct tggcaggccg tctgaaattc ggctaaggaa
      661 atatcggcgc gtgcggcagc gatgatttcg ccttcggcat cacccgtgct gctggcttcg
      721 ccgagtttca gcgtggcggt gtaggctttg tcggcatcca gcaggtattg ggcgaacttg
      781 gtcgcttcgc cgaagcagac gggcaaaagc ccggttgcca aagggtcgag cacgccggta
      841 tgtccggctt tttcggcacg gaacaaacgc cgcgcttttt gcagcgcggt gttgctggaa
      901 aggccttcgg gtttgtccaa gagcaaaaca ccgttgacgg cacgtttttg gggtttgcct
      961 gtattcatgg ttcgatgttc ggacggcatg ggcatatccg tcagtcttca accggttttt
     1021 ccgccgctac ttggtcgata agggcggaca ggttcaaacc gcgttccaag gattcgtcgt
     1081 atttgaaatg cagttcgggg attttgaaca gcttgatgcg tttggccaat tcgctgcgga
     1141 ggtgtccgcg cgcgtgttcc agcacttctt ccgtaatttc gcgcgcgtct tggtttaaaa
     1201 tggtgtagaa cacggtggcg tggctgtaat cgcgggtaac ttcgacttcg ttgacggtaa
     1261 tgaagccggc gcgcgggtct ttcagtccgg tacggacgag ttcggcaagc tcgcgcataa
     1321 tttgttcttt gacacggtct tggcgggcat agccgcgttg gggttttctc atgaatatcc
     1381 tcagatttca gatttggttt cagacggcat ttttgccgcc ggggcggttg cgaaagggcg
     1441 tattatagcc taatcggcgg gattttaagg taatacattg ataagaacgg atatagttat
     1501 tatacttggc aacccgaacg gcacgtgccg ttttctgtaa cgcatacatt gtaaagaaag
     1561 ccatgtccaa cgaaaaccgt acctgttcct tttgcggaaa atccaaatca cacgtcaaac
     1621 atttaattga gggcgaaaac gcctttatct gtgacgaatg cgtctcaaac tgtattgaaa
     1681 tattgcatga agacggaaat gacggcacgc cttcggaaag tgccggaggg gagccggaag
     1741 aatccggcaa gctgcccacg cccgccgaaa tcgtcgccaa cctcaacgac cacgttatcg
     1801 gtcaggaaca agccaagaaa gccttggcgg tgtcggtgta caaccattac aaacgcctgc
     1861 gccacccgaa agccggagct aatgtcgaat tgtcgaaatc caacatcctg cttatcggcc
     1921 cgaccggatc gggtaaaacc ctgctggcgc aatctttggc gcgcaaactg gatgtgccgt
     1981 tcgtgatggc agatgcaacc acgctgaccg aagcaggtta tgtcggcgaa gatgtcgaac
     2041 agattattac caaactgttg ggcaaatgtg atttcgatgt cgaaaaagcc caacgcggca
     2101 tcgtctatat cgacgaaatc gataaaattt cacgcaaaag cgacaatccg tccatcacgc
     2161 gtgacgtgtc cggcgaaggc gtgcaacaag ccttgctgaa actgattgaa ggtacggtgg
     2221 ccagcgttcc gccccaaggc ggccgcaagc atccgaatca ggaatttatc aacgtcgata
     2281 ccaccaacat cctgtttatc tgcggcggcg cgtttgcagg cttggaaaaa gtcatccgcc
     2341 agcgtactga aaaaggcggt atcggtttcg gtgcgtccgt tcacagcaag gacgaaaatg
     2401 ccgacattac caagctgttc ggcatcgtcg aaccggaaga tttaatcaaa ttcggtctga
     2461 ttccggaatt aatcggacgt ttgcctgtga ttgcaacgtt agaagaactg gatgaggacg
     2521 cgctgattaa tattttgacc gagccgaaaa acgctttggt caagcagtat caagccttgt
     2581 tcggtatgga aaatgtcgag ttggaatttg aagaaggcgc attgcgttcc attgcgtggc
     2641 aggcaatgga gcgcaaaacc ggcgcgcgcg gcctgcgttc catcgtcgaa cgctgcctgc
     2701 tcgatacgat gtaccgcctg cctgatttgc aaggcttgaa aaaagtggtg gtcggtaagg
     2761 cagtcatcga agagggcaag gaaccggaat tggtgttcga gtcctgatgg caaacaaaac
     2821 cgcttcggaa cgttgaacca cgtttccgaa gcggtttttt gtttttgatg cctaggggcg
     2881 gtttgcggcg gcaaattccg tcagcacgtg ttccaaacgc cgcaaacctt ccgccgtatc
     2941 ctcatcgtcc aacagcagcg aaggcgcgaa acgcaatacg tccgcacccg caaccaaaat
     3001 catcacgccg tgtttcaagg cggcggcggt gatttcggat gcgcgtccgc gataggcttc
     3061 gtccaacacg cagccgagca gcagtcccat gccgcgaacc tgtgagaaca agcctgtttt
     3121 cctgcccaaa tccagcaatg ccgtctgaag tttctgcccc tgttcacgga catggtttaa
     3181 agtttcgggc gtattgatga tgtcgaatgc gcggctgccg accgcacacg ccatcgggtt
     3241 gccgccgaaa gtcgagccgt gcgtgcccgg ttggaaggcg gcggcaatct tttctgtcgc
     3301 cagcatcgcg ccgatcggaa agccgcagcc caaggctttt gccgaactca aaatatcggg
     3361 aacaatgccg taatgttcat aggcaaacag cctgcccgta tgccccatcc cggtttgaac
     3421 ttcgtccaaa atcaacaacg cattgtgccg gtcgcacaga cggcgcgcgg tttgcaaata
     3481 ttccgcagtg gcgggcagga tgccgctttc gccctgtatc ggctcgatga tgaccgcgca
     3541 ggtctgttcg ccgacggcag cttccagcgc ggcaatatcg ttgaacggaa cgtgcgtaat
     3601 gccttgcggc agcggtgcat aatccttgct gtatttcggc tggccgccga cggacacggt
     3661 aaacagcgtg cgcccgtgga aactgttgat acaggcgacg atttcgcttt tgcctccgcc
     3721 gaaacggtcg cgcgcatatt tccttgccag cttcaacgcc gcctcattcg cctccgcgcc
     3781 cgagttgcag aaaaaaacct tgtcggcaaa actgtttgca accaattttt gcgccaattc
     3841 ctgcgccgga cgcgtcgtat agatattgga aatgtgccac agcttgtgca tctgcgcgtt
     3901 taaagcatcg acaagggcag ggtggcagtg tcccagcgca ttgacggcga taccgcctga
     3961 aaaatcaata tattcacgcc cttccgtatc ccaaacgcgg ctgcctgaag cgcgttcggg
     4021 aatcatcggg gcaaaggcga aattgggggt cagatagttt tgcataattg ctcctgattg
     4081 cggttttgct cgggattatg caccgttttt caaagcagat aaagggaaat tgccgacaat
     4141 atcgggcagc ggtaaaaata aaccgaaata ttttcaatcg gatagatgaa aaatcaaaaa
     4201 aatttcgata aaatgcttga ggcgcggcag cgttttgatt tagaatctcc ccaatatttt
     4261 tatcttcaga cggcatttca tcatgcaagg caaaaaattt acaaccgctt attgctattg
     4321 gtaccacaca gacgtgcggt acttttttgc gcttgcttga aatccatccc atacgcaaaa
     4381 caggccgctc gaaaacgggg cggctttttt gttaccttca cacaggtcgc ccgattcggc
     4441 aacacacgac agaatcagga aaatgaccat gaatgcaatg ccccaaaccg ccgcacaaaa
     4501 cgaaaccctg cacatttgca gcattattgt cgatgatgca caggattttt cccccatgct
     4561 tctcgatgca gggcgggacg acacacttat cagcgaatcg atgattccgc aaatccgcac
     4621 tgttgccgcc ctgatagccg ccgaacggca cgacttcagc cgcagcagcc ccgccgagtt
     4681 taccgatgcc gccgattttt ttgccgcacg gattctcgta ttgggcgtgc gacgcttcca
     4741 tctggacgta tcgctgcttc agattttgaa aaccgccaac agccgcgccc gccgctttgc
     4801 cgaaaaacac cgcctgtttt tcacacccgc acaagccgaa ttgagcctga acaaacacaa
     4861 aaaccgccgc ctgctgaccg tagaaaccga acacgaagtt gaaaacaaag gcaacccagt
     4921 cgccaacagc ctcgcatttg tccgcaaact gcacaccctg cccctgtaaa catcacgatt
     4981 tgtcggcaga atgcttgcca aaattcccga cggcgataca ataaaaccga ttttccaccc
     5041 aaacaggaca cacatgaaaa aaatcatcgc ctccgcgctt atcgcaacat tcgcactcgc
     5101 cgcctgccaa gacgacacgc aggcgcggct cgaacagcag cagaaacaga ttgaagccct
     5161 gcaacagcag ctcgcacagc aggcagacga tacggtttac caactgactc ccgaagcagt
     5221 caaagacacc attcctgccg aagcacaggc aaacggcaac aacgggcaac ccgttaccgg
     5281 taaagacggg cagcagtata tttacgacca atcgacagga agctggctgc tgcaaagcct
     5341 ggtcggcgcg gcggcaggcg cgtttatcgg caacgcgctg gcaaacaaat tcacacgggc
     5401 aggcaaccaa gacagtcccg tcgcccggcg cgcgcgtgca gcctaccatc agtccgcacg
     5461 ccccaatgcg cgcaccagca gggatttgaa cacgcgcagc ctccgtgcaa aacaacaggc
     5521 ggcgcaggcg cagcgttacc gcccgacaac gcgcccgccc gccaattacc gccgccccgc
     5581 tatgcgcggt ttcggcagga ggcggtaaac ccggcgcacc aatgccgtct gaagagcttt
     5641 cagacggcat ttttgcattt gttagggaca ttgttatgtt gccgtttgat tttcagacgg
     5701 cattttgttt ccaagcgttt gatgtcggga tggcaattct gatttacgtg cgttggcagg
     5761 gcggcattgt catgccgccg ttgggttttc agacggcatc atatcgaaca gtcaagccgt
     5821 agggcgagca ttcttgccca ccgttgcaag ggcgggattt ggtgggcagg aatgcccacc
     5881 ctgcatctga aacccttccg tgtcattccc gtgcaggaag gaatctagat atttgaattt
     5941 acggaaattt tcaatcgttg ccaaagcgga aaagggttt
//
LOCUS       DAXHSJ010000123         5968 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000123 DAXHSJ010000000
VERSION     DAXHSJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.123"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            71..1609
                     /gene="murJ"
                     /locus_tag="ACFM8U_001618"
     CDS             71..1609
                     /gene="murJ"
                     /locus_tag="ACFM8U_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC6334832.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIHHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFFLLRRHGIYRPGKGWAAFLAKMLL
                     ALAVMCGGLWAAQAYLPFEWVRVGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            1712..2668
                     /locus_tag="ACFM8U_001619"
     CDS             1712..2668
                     /locus_tag="ACFM8U_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HFC6334833.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGGSGSHYAVELMLAVRSRGWNGVVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGKKALPHAAA
                     AVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTTFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            2717..3499
                     /gene="trpC"
                     /locus_tag="ACFM8U_001620"
     CDS             2717..3499
                     /gene="trpC"
                     /locus_tag="ACFM8U_001620"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC6334834.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPPEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADDIEAEVGKLF"
     gene            3565..5865
                     /gene="recQ"
                     /locus_tag="ACFM8U_001621"
     CDS             3565..5865
                     /gene="recQ"
                     /locus_tag="ACFM8U_001621"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC6334835.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGKQEAIVNALAVGESLMVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADGAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQRLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVARFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         1297 a         1779 c         1634 g         1258 t
ORIGIN      
        1 aatttaatcc actatcaaag tctgatcctg ccgccctaaa gggcggggtt tcaaccgaaa
       61 aggaaatacg atgaatatgc tgggagcttt ggcaaaagtc ggcagcctga cgatggtgtc
      121 gcgcgttttg ggatttgtgc gcgatacggt cattgcgcgc gcattcggcg cgggcatggc
      181 gacggatgcg ttttttgtcg cgttcaaact gcccaacctg cttcgccgcg tgtttgcgga
      241 gggggcgttt gcccaagcgt ttgtgccgat tttggcggaa tataaggaaa cgcgttctaa
      301 agaggcgacg gaggctttta tccaccatgt ggcggggatg ctgtcgtttg tgctggtcat
      361 cgttaccgcg ctgggcatac ttgccgcgcc ttgggtgatt tatgtttccg cacccggttt
      421 tgccaaagat gccgacaaat ttcagctctc catcgatttg ctgcggatta cgttccccta
      481 tatcttattg atttcacttt cctcttttgt cggctcggta ctcaattcct atcataaatt
      541 cagcattccg gcgtttacgc ccacgttcct gaacgtgtcg tttatcgtat tcgcgctgtt
      601 tttcgtgccg tatttcgatc ctcccgttac cgcgctggct tgggcggttt ttgtcggcgg
      661 tattttgcag ctcggctttc agctcccttg gttggcgaaa ctgggttttt tgaaactgcc
      721 caaactgaat ttcaaagatg cggcggtcaa ccgcgtcatg aaacaaatgg cgcctgcgat
      781 tttgggcgtg agcgtggcgc agatttcttt ggtgattaac acgattttcg cgtcttatct
      841 gcaatcgggc agcgtttcat ggatgtatta cgccgaccgc atgatggagc tgcccagcgg
      901 cgtgctgggg gcggcactcg gtacgatttt gctgccgact ttgtccaaac actcggcaaa
      961 ccaagatacg gaacagtttt ccgccctgct cgactggggt ttgcgcctgt gcatgctgct
     1021 gacgctgccg gcggcggtcg gactggcggt gttgtcgttc ccgctggtgg caaccttgtt
     1081 tatgtaccga gaattcacgc tgtttgacgc gcagatgacg cagcacgcgc tgattgccta
     1141 ttctttcggc ttaatcggtt tgattatgat taaagtgttg gcacccggct tctatgcgcg
     1201 gcaaaacatc aaaacgcccg tcaaaatcgc catcttcacg ctcatctgca cgcagttgat
     1261 gaaccttgcc tttatcggcc cactgaaaca cgtcggactt tcgcttgcca tcggtctggg
     1321 cgcgtgtatc aatgccggat tgttgttctt cctgttacgc agacacggca tttaccgccc
     1381 cggcaagggt tgggcggcat ttttggcgaa aatgctgctc gcactcgccg taatgtgcgg
     1441 cggcctgtgg gcggcgcagg cttacctgcc gtttgaatgg gtacgcgtcg gcggaatgcg
     1501 gaaagcgggg cagctctgca tcctgattgc cgtcggcggc ggactgtatt tcgcctctct
     1561 ggcggctttg ggcttccgtc cgcgccattt caaacgcgtg gaaagctgac cggagcgccg
     1621 ccttttgccg gcaaacgctt tttccgcgcg tccgtgccgg acgggctgcc gcatcatcaa
     1681 tgccatctga aaacagaaaa accgatacct tatgattttg acaccgccgg acacaccgtt
     1741 tttcctccgc aacggcaatg ccgatacgat tgccgccaaa ttcctgcaac gccccgcacc
     1801 cgcataccgc cgcgagctgc ttcccgacag cacgggtaaa accaaagtcg cctacgactt
     1861 ttcagacggc atttcgcccg atgcgccgct ggtcgtgctg tttcacggtt tggagggcgg
     1921 cagtggcagc cattacgcgg tcgaactgat gctcgcggta cgcagccgcg gttggaacgg
     1981 cgtagtcgtc catttccgca gctgcggcgg cgtagcgaac accgccccgg tgttctacca
     2041 cttgggcgat accgccgaaa tcgcctttac tttggacacg ctcgccgcgc gttaccgtga
     2101 aatatacgcc gtcggcgtat cgctgggcgg caacgcgctg gcaaaatatt tgggcgaaca
     2161 gggcaaaaag gcattgccgc acgccgctgc cgccgtatcc gcccccgtcg atgcagaggc
     2221 ggcaggcagc cgcttcgaca gcggcatcac gcggctgctc tacacgcgct acttcctccg
     2281 caccctgata cccaaagcac gttcgctcca aggctttcag acgacatttg ccgcagggtg
     2341 caaaacactg ggtgagtttg acgaccgttt caccgcaccg ctgcacggct ttgccgaccg
     2401 gcacgactac taccgccaaa cttcctgcaa accgctgctc aaacacgttg ccaaaccgct
     2461 gctcctgctc aatgccgcca acgacccctt cctgccgccc gaagcgctgc cgacaccgga
     2521 cgaagtgtcc gaagccgtta ccctgttcca gccggcacac ggtggtcatg tcggctttgt
     2581 cagcagcacc ggcggcaggc tgcacctgca atggttgccg cagaccgtcc tgtcctattt
     2641 cgacagcttc cgtacaaaca ggcgttaacg gtttgatgct aatattcccc tttcccagac
     2701 gaatacggaa cccgacatga ccgacatcct caataaaatc cttgccacca aagcacagga
     2761 agttgccgcc caaaaagccg ccgtcaacgc cgaacacatc cgcgcacttg ccgcagaagc
     2821 cgcgcccgtc cgcagcttca tcgattcgat acgcggcaaa caccgcctaa acctgcccgc
     2881 cgtcattgcc gaaatcaaaa aggcaagccc gagcaaaggc ctgatccgtc cggacttccg
     2941 accgcccgag attgcccgcg cctatgaaaa cgccggggcg gcgtgtttgt ccgtactgac
     3001 cgacgaaccc tatttccaag gttcgcccga atacctcaaa caggcgcgcg aagccgtatc
     3061 gctgcccgtg ctgcgcaaag acttcatcat cgacgaatat caggtttatc aggcgcgcgc
     3121 atggggggcg gatgccgtcc tgctgattgc cgcagcactg gaacaggaac aattggaacg
     3181 ctttgaagcg gtggcgcacg aattgggcat gaccgtcctg ctcgagctgc acgacgaaac
     3241 cgaattggaa aaatgccgca acctgaccac gccgttgtgg ggcgtaaaca accgcaacct
     3301 gaggactttt gaagtctccc tcgaccaaac cctgtcgctg ctgcccgcac tggaaggcaa
     3361 aaccgtcgtt accgaaagcg gcattacggg caaggcggat gtggaattta tgcaatcgcg
     3421 cggcgtgcac accttcctga ttggcgaaac gttcatgcgt gccgacgata ttgaggcgga
     3481 agtgggcaaa ctcttctaaa tctcgatttc agacggcata ttgccgccga ccaatcgaca
     3541 cccattaccg gacacggaac catcatgcac cgacccaccg ccaaacagat tctgcacgaa
     3601 gtattcggtt atcccgaatt tcgcggcaag caggaggcta tcgtcaatgc tttggcagtc
     3661 ggcgagagtt tgatggtgct gatgccgacg ggcgggggca agtctttgtg ttaccagatt
     3721 ccggcgctga tgcgcgaagg cgtggcggtt gtcgtatcgc cgctgattgc gctgatgaac
     3781 gaccaagtgg ccagcctgca tgtggccggc atcgaagcgg cggcagtcaa cagcggcaca
     3841 tcggcagatg gggcgcgcga gattgccgac aagcttgccc aaggccgtct gaaactgctt
     3901 tatgtcgcac cggaacgcct agttaccgac cgctttttgc gttttctcga ccaacaaacc
     3961 gtcagcctgt tcgccattga cgaggcgcac tgcgtcagcc agtggggaca cgatttccgc
     4021 cccgaatatc aacggctcgg tatacttgcc gaacgctatc cgaacgttcc gcgcatcgcc
     4081 ctgaccgcca ccgccgatgc cgccacgcgc gccgacatca agcattatct gcacttggac
     4141 gatgcgcccg aatttgtctc cagctttgac cgcccgaata tttattatca ggttatcgaa
     4201 aaaaacaacg gcaaaaaaca attgctggat ttcatccgca aagaaatgac ggggcaaagc
     4261 ggcattgtgt attgcctaag ccgcaaaaag gttgaagatg tggcgcggtt tttgcgtgaa
     4321 aacggattaa acgcgattcc gtatcatgcc ggtctgagta tggacgtgcg cgaggaaaac
     4381 caacgccgct ttacgcatga agacaatatt atcgtggtgg cgaccgtggc gttcggcatg
     4441 ggcatagaca aacccgacgt gcgctttgtc gcccatctcg atatgcccca gagtgtcgaa
     4501 catttctatc aggagtcggg gcgcgccggt cgggacgggc tgcctgccgc gagctggctg
     4561 tgttacggtt tgaacgattg ggtgttgctg cgcgaacgga ttgccgaagg caacagcgac
     4621 gaggtgcaaa agcaaatcga aatgcaaaaa ctcgatgcca tgcttgccgt ctgcgaaacc
     4681 gccgcctgcc gccgcgtact gctgctcaaa catttcggcg aagcatccga accctgcggc
     4741 cattgcgaca actgcctgca tccgcccgta cggtttgacg gcacggtgtt ggtgcaaaaa
     4801 ttactcagct gcgtgtaccg cgccggacaa cgttttgccg ccggttacat caccaacctt
     4861 ttgcgaggta aaagcgacga ttggatacgc ggcaaccggc acgaacaact gtccacattc
     4921 ggcatcggta cggagttgtc cgacaaagaa tggcgcagcg tcatccgcca gtgtatcagc
     4981 ctcggctacc tcaccgtcaa cattacccga tatcaggcat tgcaactgac cgaagccgcc
     5041 aaaaaagtcc tcaaaggcga aaccgaagtg atgctgcgtc cgctcaagcg cgacaagccc
     5101 gccgcccgca ccctcaaaga caactggctg cgtaccgaac gcgaagaacg cctgtggcag
     5161 gcattgcgcg tttggcgtat gaaacaggca gaagccgaag gcatccccgc ctatatgatt
     5221 ttcggcgaca aaaccctgcg cgaccttgtc gaaaaaatgc cgcaaaacct caacgggctg
     5281 cacgacatct acggcttggg cgaagccaaa accgaacgtt tcggacacgg catactcgaa
     5341 gtctgccgca acgccgccgg cttcagccgc gacgcggtca tccgtccgca aaccgaacgc
     5401 gaacaacaac tgcgtcaaaa actcgaggcc tggcggtatg aacaggcaag ggcggaaaac
     5461 tgcgccctgc atgccgtcct ctccgacgaa agccttgccg atatgcttgc cgatacgccc
     5521 gaaaccgaaa ccgacctcga aggcgtacac ggcttgggca gcgtacgcgc cgccaaatac
     5581 ggacgggaca tcctcgccgt ctgccgtccg ttttcagacg gcatcgatga aaccgccaaa
     5641 cacaaacgcc gcctgatgcg cgccctgatc caatggtgca acgaaacggc aaaacacgaa
     5701 cagtccgaac cctaccgcat tctcagcaaa gccgccctac gcgccattgc cgccaaacag
     5761 ccggaaggtt tggcggagct tgccgccgta tacggcgtag gcgaagaaaa agccgcacgt
     5821 tacggtgcgg cggtgttggc ggtattggaa cgaaatgccg tctgaagccc gttaccaagt
     5881 ttcagacggc attgcctcta tttaaaaatt cctgttttta tagtggatta acaaaaatca
     5941 ggacaaggcg gcgagccgca gacagtac
//
LOCUS       DAXHSJ010000124         5947 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000124 DAXHSJ010000000
VERSION     DAXHSJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.124"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            22..405
                     /locus_tag="ACFM8U_001622"
     CDS             22..405
                     /locus_tag="ACFM8U_001622"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC6334836.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAIRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            519..992
                     /locus_tag="ACFM8U_001623"
     CDS             519..992
                     /locus_tag="ACFM8U_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC6334837.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1118..2221
                     /gene="mnmA"
                     /locus_tag="ACFM8U_001624"
     CDS             1118..2221
                     /gene="mnmA"
                     /locus_tag="ACFM8U_001624"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC6334838.1"
                     /translation="MNTTANTTNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQSTDKPVIITR"
     gene            2292..3962
                     /locus_tag="ACFM8U_001625"
     CDS             2292..3962
                     /locus_tag="ACFM8U_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC6334839.1"
                     /translation="MEKIWLDSYEQGVNAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETISFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITDTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVRGPQVMKGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLIVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVRKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4074..5708
                     /locus_tag="ACFM8U_001626"
     CDS             4074..5708
                     /locus_tag="ACFM8U_001626"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC6334840.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGVHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     TGSLAAKIYGSGHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         1554 a         1612 c         1504 g         1277 t
ORIGIN      
        1 gaacattttg aaaggatacc gatggaacct tcctcctacg cggcagaaaa aaaaggcaaa
       61 ggcggcatca ggcgcgtcat taacgcattc ggctattcga tagacggcat cgccgccgcc
      121 taccgttacg aagcggcatt ccgtcaggtt ttgtggctga acgtactgct ggtgtgcgcg
      181 gcattttttt gggtttccga aaccgccatc cgcctgccgt tgattatcgc gtcttttgtg
      241 tcggtcattg tcgaactgtt caacaccgcc gtcgaagctg ccgtcgatca tacttcgacc
      301 gaaaaacacg agctggccaa acgcgccaaa gacgcaggtt ctgccgcaca attgttcgcg
      361 atgctgatgt tggcggcggt ttggctgtct gccctgttcg ggtaaaacgc ttgcagcagg
      421 atttgtaatc ctttaggatt agtatgtggt atttattatt cacttaatcc actataagat
      481 taagcacatt ccccgtctga tattcaagga gcagaaagat gaaaaaatta ttggcagccg
      541 tgatgatggc aggtttggca ggcgcggttt ccgcggccgg agtccacgtc gaggacggct
      601 gggcgcgcac caccgtcgaa ggtatgaaaa taggcggcgc gttcatgaaa atccacaacg
      661 acgaagccaa acaagacttt ttgctcggcg gaagcagccc cgttgccgac cgcgtcgaag
      721 tgcataccca catcaacgac aacggcgtga tgcggatgcg cgaagtcgaa ggcggcgtgc
      781 ctttggaggc gaaatccgtt accgaactca agcccggcag ctatcacgtg atgtttatgg
      841 gtttgaaaaa acaattaaaa gagggcgaca agattcccgt taccctgaaa tttaaaaacg
      901 ccaaagcgca aaccgtccaa ctggaagtca aaatcgcgcc gatgccggca atgaaccacg
      961 gtcatcacca cggcgaagcg catcagcact aatctgctga aaatgtttga aatgccgtct
     1021 gaaaaagccc gggctttcag acggcatttt tatgaccgcc tttaaaatgt gttaaaatct
     1081 gcctttaaaa accgccgttt ccaaccatat tctacgcatg aatacgacag caaacaccac
     1141 caatatcatc gtcgggctct ccggcggtgt cgattcttcc gtaaccgccg ccctgctcaa
     1201 gcagcagggt tatcaagtgc gcggtgtgtt catgcagaac tgggaagacg acgacaacga
     1261 cgaatattgc agcatcaaac aggattcgtt cgatgccatc gccgttgccg atattatcgg
     1321 catcgatatc gacatcgtta atttcgccgc gcaatataaa gacaaagtct ttgcttattt
     1381 tcttcaggaa tacagtgcgg ggcgcacgcc gaatccggat gtgttgtgca acgccgaaat
     1441 caaattcaaa tgctttttgg actacgccgt agggcagggc gcggatacca tcgccaccgg
     1501 ccactatgcg cgcaaagaag ttcgcaacgg cgtgcattac ctgctcaaag gtttggatcg
     1561 aaacaaagac caaagctatt tcctctaccg cctcaagcct ttccaactcg aacgcgcgat
     1621 ttttccgttg ggcggtttgg aaaaacccga agtgcgccgc cttgccgccg agttcaaact
     1681 gccgaccgcc gccaaaaaag acagcacggg catctgcttc atcggtgaac gcccgtttcg
     1741 cgagtttctg caaaaatacc tgccgaccga caacggtaaa atggttacgc ccgaagggaa
     1801 aaccgtcggc gaacacgtcg ggctgatgtt ctacacgctc ggacagcgca aaggtttggg
     1861 catcggcggc gcgggcgaac cgtggtttgt tgcggctaaa gatttgacga aaaacgaact
     1921 gattgtcgtg caaggacacg accatccgct gctctatacc cgcagccttg tgatgaacga
     1981 tttgagtttc acgcttcccg aacgcccgaa ggcaggacgc tatacctgca aaacgcgtta
     2041 ccgtatggcg gacgcgcctt gtgagttgcg ctatttggat gaagaaaccg ccgagctggt
     2101 gtttgacgaa ccgcaatggg cggttacgcc gggccagtct gccgtgctgt acgacggcga
     2161 catctgtttg ggcggcggca ttatccaatc aaccgacaaa cccgtcatca tcacacgata
     2221 aaggtaatgc cgtctgaaac ggttttcaga cggcattgtt ccgctcaatt ccactttaaa
     2281 gaccgatacc tatggaaaaa atctggttag acagttacga acagggcgtg aatgccgaaa
     2341 tcgacatcac gcaatacggt tccgtcagcg atgtattttg ccaaagtgtc aagaaatttg
     2401 ccggccaacc tgcttttcaa aacatgggca agacgctgac ttatgccgaa accggcaaac
     2461 tggcaacaga ttttgcttct tatctgcaaa acgtcctgaa gctgccgcgc ggcgaacgtg
     2521 ttgccattat gctgcccaac gtattgcaat acccgattgc ccttttcggc attttgcagg
     2581 caggtttggt agcggtgaac accaatccgc tctatacccc gcgcgagctg gagcatcagt
     2641 tgaaagacag cggcgcgacc gccatcatcg ttttggaaaa tttcgccaac acgctggagc
     2701 tggtgctgcc gcgcacgcag atcaaacacg tcatcgtagc ctccgtcggc gaaatgttcg
     2761 ggctgcttaa aggttcgctg atcaatttcg tcatccggaa aatcaagaaa atggtgcccg
     2821 aataccgtat tcgggaaacc atttcctttc agacggcatt gaaagagggg gcgaagcacg
     2881 ttttccaacc tgtcgcatta aaccgcgaag ataccgcgct gttgcaatac acgggcggca
     2941 cgacaggtgt tgccaaaggg gcggtgctga gccacggcaa catctgcgcc aatatgcttc
     3001 aggcaaaaga atggattaaa aaccagttgc gtgagggaaa agaaaccgtt atcgccgcct
     3061 tgccgctgta ccatattttt gccttaaccg tgaatctgat gatttttgcc aatgccggct
     3121 cgaaaattgt cctgattacc aacccgcgcg atatgaaagg ctttatcgga gaactgaaaa
     3181 aacagcgggt taatgtattt atcggcgtga acacgctgtt taacgcgatg gttaaccgtc
     3241 ctgattttgc cgaagtcgat ttttcaggat tgcggctgac tttgggcggc ggtatggcga
     3301 cccaaaaagc cgttgccgaa aaatggaaaa aaatcaccga cacgcccatc gtcgaagcct
     3361 acggtctgac cgaagccagc cccggcgtgt gctgcaaccc cttaaacatc gaatcataca
     3421 gcggcagcat cggtttgccc gtctcgtcca ccgaagtcga actgcgcgac gcaaacggca
     3481 aagaagtccc cgtcgggcag ccgggcgaat tgtgggtgcg cggcccgcaa gtcatgaaag
     3541 gctattggaa ccgtcccgaa gaaaccgcta aagccataga cgcgcgcggt ttcttggaaa
     3601 ctggcgatat tgccgtcatg gacgaaaaag gctggttgaa gctggttgac cgtaagaaag
     3661 acctcattgt tgtttctggt tttaatgttt atccgaacga aatcgaggaa gtcatcgcgc
     3721 atcacggcaa agtgatggaa gtcgcctgta tcggcgttcc cgacgaaaaa accggagaag
     3781 ctctcaaagt gtttgtcgtc agaaaagatc cgtctttgac gaaagaagaa cttatcgcct
     3841 tttgccgcac cgaattaacc gcatataaag taccgaaaaa tatcgaattc cgcgacgagt
     3901 tgcccaagtc caacgtcggc aaaatcctgc gccgcgagtt gcggcaaagt actgggaaat
     3961 aaagaaaaga tgccgtctga aaacagccgt ccaccgttca gacggcatcc gtccgtttgc
     4021 cagaaccgcg cgcttcaagt taaaatcgca cattccaaca tgggtattcc atcatgacca
     4081 aattcatttt cgtaaccggc ggcgttgtct cctcactggg taaaggtatc gccgccgctt
     4141 ctattgccgc tatcctcgaa tcgcgcggct tgaacgttac catgctcaag cttgatcctt
     4201 atatcaacgt cgatcccggc acgatgagcc cgtttcaaca cggcgaagtg ttcgtaaccg
     4261 acgacggcgc ggaaaccgac ctcgacttag gacactacga acggtttatc gattccacga
     4321 tgacccgccg caacagcttc agcacggggc aggtgtacga aaacgtcatc gccaaagaac
     4381 gacggggcga ctacctcggc ggcacggttc aagtcatccc gcacattacc gacgaaatca
     4441 aacgccgcat ccacgaaggc gcggcaggtt acgatgtggc gattgtcgaa atcggcggca
     4501 cggtcggcga catcgaatcg ctgccgtttt tggaagccat ccgccagatg cgaagccagt
     4561 tgggacgcaa caacaccctg ttcgcccact tgagctacgt cccctacatc gccgctgcag
     4621 gcgaaatcaa aaccaagccg acccagcaca ccgttaaaga aatgttgagc atcggtttgc
     4681 aacccgacat cctgatttgc cgtatggaca ggacaatgcc tgcggacgaa cgccgcaaaa
     4741 tcgccttgtt ctgcaacgtg gaagaacgcg cgattgtcgg cagctacgat gtggacagca
     4801 tctacgaatg ccccgaaatg ctgcacgacc aaggcatcga caacatcatt accgagcagt
     4861 tgcagcttaa tgtgcagcag gcggatttga ccgcgtggaa aaaaatcgtc cacgccatcc
     4921 aaaacccgaa acacaccgtc aaaatcgcca tggtcggcaa atacgtcgat ttgaccgaat
     4981 cctacaaatc actgattgaa gccttgaaac acgcaggtgt tcacaccgaa accgacgtac
     5041 aaattacctt tgttgacagc gaaagcatcg agaaaaacaa cggcgacgtt tctatgctca
     5101 aagatatgga cgccatcctc gttcccggcg gtttcggttc gcgcggcgtg gaaggcaaaa
     5161 tcgccgctgt gcgctatgcc cgcgaaaaca acgtgccata cttgggcatc tgcctcggta
     5221 tgcagattgc gctgatcgaa tacgcccgcg acgtggcagg tttgaaaggt gcgaattcca
     5281 ccgagttcga cctcaaatgc gccgcccccg tcgttgccct gattgacgaa tggcaaactg
     5341 ccgacggcag cgtcgaaacc cgcgacgaat ccgccgattt gggcggcacc atgcgtttgg
     5401 gcgcgcaaga agtcgaattg aaaacaggca gcctcgccgc caaaatctac ggcagcggac
     5461 acatccgcga acgccaccgc caccgctacg aagtcaacaa caactatgtt cctacgctgg
     5521 aacaggcagg tttggtcatc ggcggcgtat ccgccggacg cgaacgcttg gtcgaaacca
     5581 tcgaactgcc gaaccatcct tggttcttcg cctgccagtt ccaccccgag tttacctcca
     5641 atccgcgcaa agggcatcct ttgttcaccg cgtttgtcaa agctgcgttg aacaataaaa
     5701 aagcctgata aagcgttact tgatgatcaa aatgccgtct gaaagcctga atgaagactt
     5761 tcagacggca ttttcgcaaa tcggggatta taaaagccat caatttaatt tttatggttt
     5821 tatctaatat ttaccaactg aatattgtta agcggggcgt gaaactcaag ttgagacctt
     5881 tgcaaaattc cccaaaatcc cctaaattcc caccaagaca tttaggagca ccttcttccg
     5941 gcaaacc
//
LOCUS       DAXHSJ010000125         5852 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000125 DAXHSJ010000000
VERSION     DAXHSJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.125"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..389)
                     /locus_tag="ACFM8U_001627"
     CDS             complement(<1..389)
                     /locus_tag="ACFM8U_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFC6334841.1"
                     /translation="MLCPEKMSVMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASLQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKL"
     gene            complement(425..538)
                     /gene="rrf"
                     /locus_tag="ACFM8U_001628"
     rRNA            complement(425..538)
                     /gene="rrf"
                     /locus_tag="ACFM8U_001628"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(635..3526)
                     /locus_tag="ACFM8U_001629"
     rRNA            complement(635..3526)
                     /locus_tag="ACFM8U_001629"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3934..4009)
                     /locus_tag="ACFM8U_001630"
     tRNA            complement(3934..4009)
                     /locus_tag="ACFM8U_001630"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3974..3976),aa:Ala,seq:tgc)
     gene            complement(4015..4091)
                     /locus_tag="ACFM8U_001631"
     tRNA            complement(4015..4091)
                     /locus_tag="ACFM8U_001631"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4055..4057),aa:Ile,seq:gat)
     gene            complement(4193..5733)
                     /locus_tag="ACFM8U_001632"
     rRNA            complement(4193..5733)
                     /locus_tag="ACFM8U_001632"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1271 a         1719 c         1262 g         1600 t
ORIGIN      
        1 agcttcgcca gcaccgcagg gccgatgccc tttaccttgg tcaaatcgtc cacagacttg
       61 aacgcaccgt tttgcgcacg gtattccgca atggccttcg ccttcgccgg gcctatgcct
      121 ggcagcgcct ccaactcctg cagcgaagcc gcattgatgt ttaccgccgc aagggagaag
      181 gcgcaggaga acagcataca gaacagcacg aacattttct tcatggtttt tcctttaagg
      241 gttgcaaaca ataaaccgca tcttgcgacg ataaaacgag tcattctaaa atgaatatcc
      301 caaagtttca agccgttcct ccgcaaaccc gaccggacac cgtacggatg ccgtcccgcc
      361 atcaccgaca ttttttccgg gcaaagcaaa aacccccgaa taatcggggg ttttctgaat
      421 gggtgtttgg cagtgaccta ctttcgcatg gaagaaccac actatcatcg gcgctgagtc
      481 gtttcacggt cctgttcggg atgggaaggc gtgggaccaa ctcgctatgg ccgccaaact
      541 taaactgtta caaatcggta aagccttaat caatatattc ggtaatgact gaatcagtca
      601 gtaagctttt atctcttgaa gttcttcaaa tgatagagtc aagcctcacg agcaattagt
      661 atgggttagc ttcacgcgtt accgcgcttc cacaccccac ctatcaacgt cctggtctcg
      721 aacgactctt tagtgcggtt aaaccgcaag ggaagtctca tcttcaggcg agtttcgcgc
      781 ttagatgctt tcagcgctta tctcttccga acttagctac ccggctatgc aactggcgtt
      841 acaaccggta caccagaggt tcgtccactc cggtcctctc gtactaggag cagcccccgt
      901 caaacttcca acgcccactg cagataggga ccaaactgtc tcacgacgtt ttaaacccag
      961 ctcacgtacc actttaaatg gcgaacagcc atacccttgg gaccgactac agccccagga
     1021 tgtgatgagc cgacatcgag gtgccaaact ccgccgtcga tatgaactct tgggcggaat
     1081 cagcctgtta tccccggagt accttttatc cgttgagcga tggcccttcc atacagaacc
     1141 accggatcac tatgtcctgc tttcgcacct gctcgacttg tcggtctcgc agttaagcta
     1201 ccttttgcca ttgcactatc agtccgattt ccgaccggac ctaggtaacc ttcgaactcc
     1261 tccgttacgc tttgggagga gaccgcccca gtcaaactgc ctaccatgca cggtccccga
     1321 cccggatgac gggtctgggt tagaacctca aagacaccag ggtggtattt caaggacggc
     1381 tccgcagaga ctggcgtctc tgcttctaag cctcccacct atcctacaca agtgacttca
     1441 aagtccaatg caaagctaca gtaaaggttc acggggtctt tccgtctagc agcgggtaga
     1501 ttgcatcttc acaaccactt caacttcgct gagtctcagg aggagacagt gtggccatcg
     1561 ttacgccatt cgtgcgggtc ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt
     1621 tatagttacg gccgccgttt actggggctt cgatccgatg ctctcacatc ttcaattaac
     1681 cttccagcac cgggcaggcg tcacacccta tacgtccact ttcgtgttag cagagtgctg
     1741 tgtttttaat aaacagtcgc agccacctat tctctgcgac cctccggggc ttacggagca
     1801 agtccttaac cttagagggc ataccttctc ccgaagttac ggtatcaatt tgccgagttc
     1861 cttctcctga gttctctcaa gcgccttaga attctcatcc tgcccacctg tgtcggtttg
     1921 cggtacggtt cgattcaaac tgaagcttag tggcttttcc tggaagcgtg gtatcggttg
     1981 cttcgtgtcc gtagacactc gtcgtcactt ctcggtgtta agaagacccg gatttgccta
     2041 agtcttccac ctaccggctt aaacaagcta ttccaacagc ttgccaacct aaccttctcc
     2101 gtccccacat cgcatttgaa tcaagtacag gaatattaac ctgtttccca tcgactacgc
     2161 atttctgcct cgccttaggg gccgactcac cctacgccga tgaacgttgc gcaggaaacc
     2221 ttgggctttc ggcgagcggg cttttcaccc gctttatcgc tactcatgtc aacattcgca
     2281 cttctgatac ctccagcaca ctttacaatg caccttcatc ggcctacaga acgctcccct
     2341 accatgccgg taaaccggca tccgcagctt cggttataga tttgagcccc gttacatctt
     2401 ccgcgcagga cgactcgacc agtgagctat tacgctttct ttaaatgatg gctgcttcta
     2461 agccaacatc ctggctgtct gggccttccc acttcgttta ccacttaatc tatcattggg
     2521 gaccttagct ggcggtctgg gttgtttccc tcttgacaac ggacgttagc acccgctgtc
     2581 tgtctcccga ggaaccactt gatggtattc tgagtttgcc atgggttggt aagttgcaat
     2641 aaccccctag ccataacagt gctttacccc catcagtgtc ttgctcgagg cactacctaa
     2701 atagttttcg gggagaacca gctatctccg agtttgttta gcctttcacc cctatccaca
     2761 gctcatcccc gcattttgca acatgcgtgg gttcggtcct ccagtacctg ttacggcacc
     2821 ttcaacctgg ccatggatag atcactcggt ttcgggtcta cacccagcaa ctcatcgccc
     2881 tattaagact cggtttccct acgcctcccc tattcggtta agctcgctac tgaatgtaag
     2941 tcgttgaccc attatacaaa aggtacgcag tcacacctct agggcgctcc cactgtttgt
     3001 atgcatcagg tttcaggttc tgtttcactc ccctcccggg gttcttttcg cctttccctc
     3061 acggtactgg ttcactatcg gtcgatgatg agtatttagc cttggaggat ggtcccccca
     3121 tattcagaca ggatttcacg tgtcccgccc tacttttcgt acgcttagta ccgctgttga
     3181 gatttcgaat acgggactgt cacccactat ggtcaagctt cccagcttgt tcttctatct
     3241 cgacagttat tacgtacagg ctcctccgcg ttcgctcgcc actacttgcg gaatctcggt
     3301 tgatttcttt tcctccgggt acttagatgg ttcagttctc cgggttcgct tctctaagtc
     3361 tatgtattca acttaggata ctgcacagaa tgcagtgggt ttccccattc ggacatcgcg
     3421 gaatcattgc tttattgcca gctcccccgc gcttttcgca ggcttacacg tccttcgtcg
     3481 cctatcatcg ccaaggcatc cacctgatgc acttattcac ttgactctat catttcaaga
     3541 acttctttga cttcgtttgc ctacctgttg actaagtaaa agcaaacttg aaattcctac
     3601 tttgataaag cttactgctt tgttgtgtct taatcctgcc ttttgtgttt caggattaag
     3661 tcgatacaat catcacccaa atactgtgtt tgttttcttt tctctcgcga gagattttta
     3721 tcctttgcaa agaataaaaa atcaaaacaa acgctttgtc tttgtttgtt gatttcggct
     3781 ttccaatttg ttaaagatcg atgcgttcga tattactatc tactgtgcaa gtcaaaacga
     3841 gctgattatt atatcagcat tttgttcttg gtcaagtgtg acgtcgccct gaatggattc
     3901 tgttccattc ttccgttttg atttgtacag tattggtgga ggcaaacggg atcgaaccga
     3961 tgaccccctg cttgcaaagc aggtgctcta ccaactgagc tatgcccccg ttcttggtgg
     4021 gtctgggagg acttgaacct ccgaccccac gcttatcaag cgtgtgctct aaccagctga
     4081 gctacaaacc cggattctct tcttaagcga atcttgcctt cactcaagct tcttccgcat
     4141 ctttttcagt ttaccgataa gtgtgaatgc ctaaagcctc ttctttctct agaaaggagg
     4201 tgatccagcc gcaggttccc ctacggctac cttgttacga cttcacccca gtcatgaagc
     4261 ataccgtggt aagcgggctc cttgcggtta ccctacctac ttctggtatc ccccactccc
     4321 atggtgtgac gggcggtgtg tacaagaccc gggaacgtat tcaccgcagt atgctgacct
     4381 gcgattacta gcgattccga cttcatgcac tcgagttgca gagtgcaatc cggactacga
     4441 tcggttttgt gagattggct ccgcctcgcg gcttggctac cctctgtacc gaccattgta
     4501 tgacgtgtga agccctggtc ataagggcca tgaggacttg acgtcatccc caccttcctc
     4561 cggcttgtca ccggcagtct cattagagta cccaactgaa tgatggcaac taatgacaag
     4621 ggttgcgctc gttgcgggac ttaacccaac atctcacgac acgagctgac gacagccatg
     4681 cagcacctgt gttacggctc ccgaaggcac tcctccgtct ccagaggatt ccgtacatgt
     4741 caagaccagg taaggttctt cgcgttgcat cgaattaatc cacatcatcc accgcttgtg
     4801 cgggtccccg tcaattcctt tgagttttaa tcttgcgacc gtactcccca ggcggtcaat
     4861 ttcacgcgtt agctacgcta ctaagcaatc aagttgccca acagctaatt gacatcgttt
     4921 agggcgtgga ctaccagggt atctaatcct gtttgctacc cacgctttcg ggcatgaacg
     4981 tcagtgttgt cccaggaggc tgccttcgcc atcggtattc ctccacatct ctacgcattt
     5041 cactgctaca cgtggaattc tacctccctc tgacacactc tagtcaccca gttcagaacg
     5101 cagttcccgg gttgagcccg gggatttcac atcctgctta agtaaccgtc tgcgcccgct
     5161 ttacgcccag taattccgat taacgctcgc accctacgta ttaccgcggc tgctggcacg
     5221 tagttagccg gtgcttattc ttcaggtacc gtcatcggcc gctggtatta gcaacagcct
     5281 tttcttccct gacaaaagtc ctttacaacc cgaaggcctt cttcagacac gcggcatggc
     5341 tggatcaggc ttgcgcccat tgtccaaaat tccccactgc tgcctcccgt aggagtctgg
     5401 gccgtgtctc agtcccagtg tggcggatca tcctctcaga cccgctactg atcgtcgcct
     5461 tggtaggcct ttaccccacc aaccagctaa tcagatatcg gccgctcgaa tagcgcaagg
     5521 cccaaaggtc ccctgctttc tctctcaaga cgtatgcggt attagctgat ctttcgatca
     5581 gttatccccc actactcggt acgttccgat atgttactca cccgttcgcc actcgccacc
     5641 caagaagcaa gcttctctgt gctgccgtcc gacttgcatg tgtaaagcat gccgccagcg
     5701 ttcaatctga gccaggatca aactcttatg ttcaatctct aactttttaa cttctggtct
     5761 gcttcaaaga aaccaacagg acaatgttca aaacattatc ttgtctgtct ttcaaacagt
     5821 gtgaggctca aggcactcac acttatcggt aa
//
LOCUS       DAXHSJ010000126         5821 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000126 DAXHSJ010000000
VERSION     DAXHSJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.126"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            158..964
                     /locus_tag="ACFM8U_001633"
     CDS             158..964
                     /locus_tag="ACFM8U_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC6334842.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            complement(1022..2287)
                     /locus_tag="ACFM8U_001634"
     CDS             complement(1022..2287)
                     /locus_tag="ACFM8U_001634"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFC6334843.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKIVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEISNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            2694..3404
                     /locus_tag="ACFM8U_001635"
     CDS             2694..3404
                     /locus_tag="ACFM8U_001635"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC6334844.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            3477..3938
                     /gene="recX"
                     /locus_tag="ACFM8U_001636"
     CDS             3477..3938
                     /gene="recX"
                     /locus_tag="ACFM8U_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC6334845.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPYAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            4012..4173
                     /locus_tag="ACFM8U_001637"
     CDS             4012..4173
                     /locus_tag="ACFM8U_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334846.1"
                     /translation="MSGQPEKNHNPLLEDERKNPVYRMGQAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            4326..4808
                     /locus_tag="ACFM8U_001638"
     CDS             4326..4808
                     /locus_tag="ACFM8U_001638"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6334847.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(5015..>5821)
                     /locus_tag="ACFM8U_001639"
     CDS             complement(5015..>5821)
                     /locus_tag="ACFM8U_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6334848.1"
                     /translation="PAGYAAPKAAAVKSRPAVPAAAQPPVQSAPVDINAATHTIVRGD
                     TVYNISKRYHISQDDFRAWNGMTDNMLSIGQIVKVKPAGYAAPKTSAVESRPAVPAAV
                     QTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGK
                     VVADFGGNNKGVDIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGH
                     NQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT         1480 a         1696 c         1377 g         1268 t
ORIGIN      
        1 tcgttctctt tgagctaagg cgaggcaacg ccgtactggt ttttgttaat ccactataca
       61 aaaagacagt tttcagacag caaatccgtc ttcacacgat acctattttg ttataacata
      121 acaaaatctt taacccacac gagacaaagg ctgcaccatg aagaaaacat tgacactgct
      181 cgccgtttcc gccctatttg ccacatccgc ccacgcccac cgcgtctggg tcgaaaccgc
      241 ccacacgcac ggcggcgaat accttaaagc cgacttgggc tacggcgaat ttcccgaact
      301 cgaacccatc gccaaagacc gcctgcacat cttcagcaaa ccgatgcagc tggttaccga
      361 aaaaggcaag gaaaacatga ttcaacgcgg cacatacaac taccagtacc gaagcaaccg
      421 tcccgttaag gacggcagtt acctcgtcat cgccgaatat cagcctactt tctggtcaaa
      481 aaacaaagca ggctggaaac aggcgggcat caaagaaatg cctgacgcaa gctattgcga
      541 acaaacccga atgttcggca aaaacatcgt caacgtcgga cacgaaagcg cggacaccgc
      601 catcatcacc aaaccggtcg gacaaaactt ggaaatcgtc ccgctggaca atcccgccaa
      661 cattcacgta ggcgaacgct tcaaagtccg cgttctgttc cgtggcgaac cgctgcccaa
      721 tgccaccgtt accgccacct ttgacggctt cgacaccagc gaccgcagca aaacgcacaa
      781 aaccgaagca caggctttct ccgacagcac agacgacaaa ggcgaagtgg acatcatccc
      841 cttgcgccaa ggcttctgga aagccaatgt cgaacacaaa gccgacttcc ccgatcaaag
      901 cgtgtgccaa aaacaggcga actactcgac tttaaccttc caaatcggcc attcgcacca
      961 ttaatcccgc ccgcacaaaa atgccgtctg aaggcttcag acggcatttt ttgttcaaac
     1021 atcaatacca accgcgcagt ttcatcgctt tttcaacacg gcggatactc atcatgtaag
     1081 acgcggttcg caaatcgaca tcatactctt gcgccaagtt ccatatatcg cggaacgcac
     1141 ggcgcaggac aacggtttct ttctcttgaa cttcgtcaaa ctcccagtag tagccttgca
     1201 ggttttgtac ccattcaaaa taggatacca ccacgccgcc gcagtttgcc aaaatatcag
     1261 gaacgaccaa tacgccgttt tgacgcagga taacgtctgc ctctggagta gtcgggccat
     1321 ttgcgccttc gaccacgatt gtcgcgcgga ctttaccggc attttcagaa gtcagttggt
     1381 tttccagtgc gcaaggggca agtacgtcca cttccagagc caaaagttcg gcattgctga
     1441 tttctttacc gtagccggct tcattggtga tgaagccttt ttcttggaac tctttaaaca
     1501 aagcttccat atccaaaccg ttttcgttgt agatggcaac gtcaacagta gaaacggcaa
     1561 cgattttcgc accggattga tgcgcgtaat aacctgtgtg gtagccgaca ttaccgaaac
     1621 cttgaatggc gtaagtggca cccttcacgt ccttgcccag tttttccaaa gcttggacgg
     1681 cggcgaggtt cacgccgtaa ccggtagcct cggtacgcgc caaagagccg ccgaactcaa
     1741 ccggttttcc ggtaaatacg cccggcgcgg aatgtttcac cacgttttca taagcatcca
     1801 ccatccacga cataattttg ccgttggtat tcacatcggg ggcgggaata tcgattttct
     1861 cgccaatcag cggagaaatg gcttcggaat aagcgcgagc gatgcgttcc agttccgcct
     1921 cggaataatc gcgcggatcc aaggtaatac cgcctttgcc gccgccgtaa ggaatgccgg
     1981 ccacgcagca tttgatggtc atccaaatcg acagggcttt gacttcgtcc agattcacac
     2041 cgggatggaa gcgcacgccg cctttatagg ggccgacggc gttgttgtgt tgcgaacggt
     2101 agcccgtgaa ggttttgacc gtgccgtcgt cgagtttgac ggggaaattg acttccaaca
     2161 cgcgggtcgg actcttcagg atttcataaa cggccggatc ggttttcagc cggtcgcagg
     2221 cggttttgac ctgtttgcgc gcgatttcaa acggattgag ggtttctttt gcaagggctt
     2281 cagacatttt gcttcctttt cacaaaaaga ttttcagggg cgtgcatttc gctcattact
     2341 ataaccaatt tcccgacaaa atgtaatagc gtgtagtcga cataagcccg atttgattaa
     2401 tctatatatg attttatttt tcaagccgca cggaatcagc ccggactccg cctgaaaaag
     2461 cagcgcgcgc gtccgaaaaa taaatgtcaa attaaataaa gatataggca tccttttatt
     2521 ttttaaaaat ttaattggaa catcgccggg atttcaaacc cttgcccgat tccgccccga
     2581 aatccgaaaa caccgcctgc aaaacctgtt gcgattgtta acaatctata cattagaagc
     2641 cctgtgcaaa cgatgttaaa ataaaccctt tcaacccgac aggaaatcag attatgaatg
     2701 cagccatcga acacgtccaa gccgtcgcct tcgatttgga cggcacactg tgcgattccg
     2761 tccccgacct tgccgccgcc gcagaagcga tgttggagca actcggtatg aaaccgctgc
     2821 ccgccaaagt ggtcgaaagc tatgtgggcg acggcatcgg caaactggtt caccgcgtcc
     2881 tcaccaacga ccgcgaccgc gaagccgatt ccgaactgtg ggaaaaaggt ttcgtgtcct
     2941 atatgaaata ctaccgcgac catttgagcg tcttcacccg cccctatccc gaaaccgaag
     3001 ccgggctggc attgctcaaa tctttgggca tcccgctggc agtcattacc aacaaaaacg
     3061 aaatccttgc cgtcgaactg ttgaaacagc tcggacttgc cgactacttc agcctgatac
     3121 tcggcggcga cagcctgccc gagaaaaaac ccagccccct gccgctgcgg catgccgccg
     3181 aagttttggg tatcgatgtt gcaaacatgg ttatggtcgg cgactcgcgc aacgacatca
     3241 tcgccgccaa agccgccggc tgcctgagcg tcggcgttac cttcggttac ggcgatatga
     3301 cgctgctctc gcaagacgat gcgacccgcc ccgactggat tatcggctcg ctgcccgaaa
     3361 tttacgaaaa cctgcaaccg cagaaaaata aagacgaaga ataaaacaat ctgttcagac
     3421 ggcatcggaa tccgtttccc atgccgtctg aatctttccg tgccgaaacc gccgccatga
     3481 aaccgcaaaa atccctacgc gcccgcgcga tggacatcct ctcgcgccaa gaagtcagcc
     3541 gcatcggtct gaaacgcaaa cttgcaccgt acgccgaaag cgaagaggag ttggaaaacg
     3601 tgttaaacga atttgccgaa cgcaactggc agtcggattt gcgctatgcc gaagcctata
     3661 tccgcagcaa aagccgcaaa cacggttcat tgaggctgaa acaggctttg gcgcaacagg
     3721 gcatagatga agaaaccagc cgcaacctgc ttcccgaccg ctcaagcgaa aaacaggccg
     3781 ccatagccgt gttgcgtaaa aaattcaaac accccgccgc cgacctcaaa gaaaaacaaa
     3841 aacaggcgcg tttcctcgcc tatcgcggat tcgatgccga taccgttcag acggcattga
     3901 aacacgcttg ggacgaaaat tgggaagacg gctgctgacc ttccttcttg aatctttttg
     3961 cacggcaaca taaccttacg cccgtttcca acttttccga ttgagaacaa aatgtccgga
     4021 caacccgaaa aaaatcataa ccccctcctt gaggacgaac gcaagaaccc cgtgtaccgc
     4081 atggggcagg cagttgccgg attcatgctc gtcgtttggg caggcgtatt ggcactcgtg
     4141 tttttcctgg tcttccgttt ttggctttcc taaacaaaat gccgtctgaa ggcttcagac
     4201 ggcatcggcg gcccatttct gcagactatc ccgccatagc tttttttagc ttgaattcca
     4261 cttccccatt ccctaaaatt ttcccacacc catttcaaaa caccctttct taaaacaggt
     4321 acactatgac acaacaacgc caactgcctt cccacgaact cattatgtcc gaactgatga
     4381 tgccggacac cgccaatttc agcggcaacg tacacggcgg cgaactcctg ctcctgctcg
     4441 accaagtcgc ctattcctgc gccagccgtt acagcggcaa ttattgcgtt accctgtcgg
     4501 ttgacaaagt cctgtttaaa gaacccatcc acatcggcga tttggttacc ttttacgccg
     4561 ctgtcaacta cacgggacgc acctcgatgg aaatcggcat ccgtgtcgaa gcacaaaaca
     4621 tccgcaccgg cgaaatccgc cataccaaca gctgctactt cacgatggtt gcggtcaaag
     4681 acggcaaacc cgtccctgtc cctccgctgg aaatcctgac cgaccgccaa cgctgccgct
     4741 acgaaaaagc caaaaaacgc agagacatca gcctgcaagc ctctgaagac atgtcctgcg
     4801 gctgctgacg gcggactatg ccgtctgaaa gacaggcaca tcgcgccatc cgtttctact
     4861 gcaaacggat gaaatcaagc aaatacccaa acacagtcaa acaaatttat atgccccgtc
     4921 ccttccgaat gatttgaaaa cacagccgcc aaaaacaaaa atgccgtctg aaaacctttc
     4981 agacggcatt tccaacttga tttcaggcag aaagtcagaa cgcgatatag ctgttcggat
     5041 taaccggttt gccgttttgc cgcacctcga aatgaagctg cgttctggaa gcgtcggtat
     5101 tgcccatcaa agcgacctgc tgcccgcgtt tgacctgctg gccttcgccg accagcaatt
     5161 tttggttgtg cccgtatgcg gtcaggaagg aagaattatg ctggatgatg accagattgc
     5221 cgtatcccct caaaccggaa cctgcataaa ccactttgcc gtcagccgcc gccaaaacgg
     5281 gctgtcccgc attaccggca atatcgacac ccttgttgtt gccgccgaaa tcggcaacca
     5341 ctttaccttg cgtcggacgc tgccaaacaa tgccgccgac cgaacgcgtg ccggaaggcg
     5401 aagcggcagg agattgcggg gcgggcgcgg gaaccgcttt attttccgca gcgggcgcgg
     5461 caggttgcgg cgcggactgc acaggcggtt gcgcggcggg tttcacaggg gtttgcacgg
     5521 cagccggtac ggcgggcctg ctttctacgg ctgaggtttt cggtgcggca tatcctgccg
     5581 gtttgacttt aacaatctga ccgatgctca acatattgtc ggtcatgccg ttccacgcac
     5641 ggaaatcgtc ttgagagata tggtagcgtt tggaaatgtt gtacaccgtg tcgccgcgca
     5701 caatagtatg cgtcgccgcg ttgatgtcga cgggtgcgga ctgtacgggc ggttgcgcgg
     5761 cagccggtac ggcgggcctg ctttttacgg ctgcggcttt cggtgcggca tatcctgccg
     5821 g
//
LOCUS       DAXHSJ010000127         5698 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000127 DAXHSJ010000000
VERSION     DAXHSJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.127"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(84..845)
                     /locus_tag="ACFM8U_001640"
     CDS             complement(84..845)
                     /locus_tag="ACFM8U_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334849.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            945..1196
                     /locus_tag="ACFM8U_001641"
     CDS             945..1196
                     /locus_tag="ACFM8U_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334850.1"
                     /translation="MSSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEVA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1248..2054
                     /locus_tag="ACFM8U_001642"
     CDS             1248..2054
                     /locus_tag="ACFM8U_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6334851.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNTMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            2361..3884
                     /locus_tag="ACFM8U_001643"
     CDS             2361..3884
                     /locus_tag="ACFM8U_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFC6334852.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTSPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGAAACEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            3982..5394
                     /gene="trkA"
                     /locus_tag="ACFM8U_001644"
     CDS             3982..5394
                     /gene="trkA"
                     /locus_tag="ACFM8U_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC6334853.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGEVIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1441 a         1711 c         1388 g         1158 t
ORIGIN      
        1 atattttgcg cctgttctat gatgctttca agtcgggtga gaatgcaaat gccgtctgaa
       61 acggctttca gacggcatgg caatcagcgt ttgtatttta actcgtactt gatgtcgttg
      121 aggattttgc ggacatcgtg ttccaacacg tcttcgacta ccgcacccgc ctgctcgtgc
      181 agcatctgct ggagctgata ggtaaaaacc gccatctgct tttgcaccgc cgtacggatg
      241 ataccgttga cggtatcggt cagatgcggg cgcaggcgtt tgatcagccg ttcggtcagc
      301 tcctgttcgg acaggcagaa cacttcgcgc cggttgacgg ctttcgggtt cagaatattg
      361 atttggacgg gcatcaacgc ttcttccgca ccgccttcgc cgttttccgc aaccccgtct
      421 gccgccgtac ccgattctgc cgcatcggcg ttttccccgc cttcaatctg tccggtttca
      481 aattcggcac tgtctttttt ggcatcaaac cggattctcc gccgcgattc gatgtgtttt
      541 tccgaaaccg acatttgcag ggaagcctgc gcgttgagcc agttttcctg aaggacgatc
      601 atcgggtcgg tttcgacttc ctcgccgcaa tcggcaacgg cggcattgtg ttcctcctgc
      661 cattttttca gatacgcctt caacacacgg gctcggctct catcgtccag tttcggcaca
      721 ggcgcgtccg ttccggtttc agaggggcgg gacagcggcg cgtaagtcgg cactgccttc
      781 atacggcgcg tctgacgcag gttttccaaa cgtttttccc aattcggctc tttattcgca
      841 tccattttcg gcttccggtt cttaatcttt gcaggcagac aaacccgcgc ccaaagcgcg
      901 gtttgatata atggcgcatt ttaacagatt cgcgaggata catcatgagc agcatcgaac
      961 agcgtttgga atatctggaa gaggcgaacg acgtgctgcg tatgcagaac cacgtcctgt
     1021 ccaccgcctt caaagcccta atccgcgccc ttcccgccga aaccgccgaa gtcgcggtcg
     1081 agtcgattca gcttgctttt gaagacgcac tggcggaatt gagctatgag gacagcccgc
     1141 atacggattt gttccacgac gttacttatg cgtttttccg tgaaaaggaa cgttaatttt
     1201 atgttaaact gattttttag gctttttgat taccgaaagg aattttgatg aatatgaaaa
     1261 aatggattgc cgccgccctt gcctgttccg cgctcgcgct gtctgcctgc ggcggtcagg
     1321 gcaaagatgc cgccgcacct gccgcaaacc ccgacaaagt gtaccgcgtg gcttccaacg
     1381 ccgagtttgc cccctttgaa tctttagact cgaaaggcaa tgtcgaaggt ttcgatgtgg
     1441 atttgatgaa cacgatggcg aaggcgggca attttaaaat cgaattcaaa caccagccgt
     1501 gggacagcct tttccccgcc ttgaacaacg gcgatgcgga cgttgtgatg tcgggcgtaa
     1561 ccattaccga cgaccgcaaa cagtctatgg acttcagcga cccgtatttt gaaatcaccc
     1621 aagtcgtcct cgttccgaaa ggcaaaaaaa tatcttcttc cgacgatttg aaaaacatga
     1681 acaaagtcgg cgtggtaacc ggctacacgg gcgatttctc cgtatccaaa ctcttgggca
     1741 acgacaaccc aaaaatcgcg cgctttgaaa acgttcccct gattatcaaa gaactggaaa
     1801 acggcggctt agattccgta gtcagcgaca gcgcggtcat cgccaattat gtgaaaaaca
     1861 atccgaccaa agggatggac ttcgttaccc tgcccgactt caccaccgaa cactacggca
     1921 tcgcggtacg caaaggcgac gaagcaaccg tcaaaatgct gaacgatgcg ttggaaaaag
     1981 tacgcgaaag cggcgaatac gacaagattt acgccaaata ttttgcaaaa gaagacggac
     2041 aggccgcaaa ataagcccgc ccgtccgaac acaatgccgt ctgaagccct ttcagacggc
     2101 attgttcatc aatcggccta caatgaactg cctgctgatt tctccctacc gcaaagcaac
     2161 aggcaaagat tacaaatatc aaaatccgag taaaacagta ttttattaaa acaaattgat
     2221 aatcaagaga ttagaattat gtattgtctt taccgtacaa acgctggcac tatttcaacc
     2281 tgataaaaaa cagccttcaa aaaggttgtt taaaacagca gcagacactt accgccacaa
     2341 ccttgaaaag gaacacaatc atgaccgtca tcaaacagga agactttatc caaagcattt
     2401 gcgatgcctt ccaattcatc agctactacc atcccaaaga ctacatcgac gcgctctata
     2461 aagcgtggca gaaggaagaa aaccccgccg ccaaagacgc gatgacgcag attttggtca
     2521 acagccgcat gtgtgccgaa aacaaccgcc ccatctgcca agacacaggt atcgcaaccg
     2581 tgtttttgaa agtcggtatg gatgtgcagt gggatgcgga catgagcgtg gaagaaatgg
     2641 ttaacgaagg cgtgcgccgc gcctacactt gggaaggcaa caccctgcgc gcctctgtcc
     2701 tcgccgatcc ggccggcaaa cgtcaaaaca ccaaagacaa cacccccgcc gtcattcata
     2761 tgagcatcgt gccgggcgac aaagtcgaag taacctgcgc ggcaaaaggc ggcggttctg
     2821 aaaacaaatc caaactcgcc atgctcaacc cttccgacaa catcgtcgat tgggtattga
     2881 aaaccattcc gaccatgggc gcgggctggt gtcctcccgg catcttgggc atcggcatcg
     2941 gcggtacgcc tgaaaaagcc gtgttgatgg caaaagagtc cctgatgagc cacatcgaca
     3001 tccaagaatt gcaggaaaaa gccgcgtccg gcgcagaatt gtccaccacc gaagccctgc
     3061 gcctcgaact ctttgaaaaa gtcaacgcgc tgggcatcgg cgcacaaggc ttgggcggac
     3121 tgaccaccgt gttggacgtg aaaatcctcg attatccgac ccatgccgcc tccaaaccga
     3181 ttgccatgat tccgaactgc gccgccaccc gccacgtcga gttcgaattg gacggctcag
     3241 gccctgtcga actcacatcg ccgcgcgtcg aagactggcc cgatttgact tacagccccg
     3301 acaacggcaa acgcgtcgat gtcgataagc tgaccaaaga agaagtggca agctggaaaa
     3361 ccggcgacgt attgctgctg aacggcaaaa tcctcaccgg ccgcgatgcc gcacacaaac
     3421 gcctcgtcga tatgctcaac aaaggcgaag aattgcccgt cgatttcacc aaccgcctga
     3481 tttactacgt cggtcccgtc gatccggtcg gcgatgaagt cgtcggtccg gcaggtccga
     3541 ccacagccac ccgcatggac aaattcaccc gccaaatgct cgaacaaacc gacctcttgg
     3601 gcatgatcgg caaatccgag cgcggcgcgg cggcgtgcga agccatcgcc gacaacaaag
     3661 ccgtgtacct catggcagtc ggcggcgcgg cgtatctcgt ggcaaaagcc atcaaatctt
     3721 ccaaagtctt ggcgttcccc gaattgggca tggaagccat ttacgaattt gaagtcaaag
     3781 acatgcccgt aaccgtcgcc gtagatagca aaggcgaatc catccacgcc accgccccgc
     3841 gcaaatggca ggcgaaaatc ggcatcatcc ccgtcgaatc ttgaggcacc atgccgtctg
     3901 aacacaaaat ctgccttcag acggcatttc cgcccccggt tgcggtacaa tccaccattt
     3961 cattactcgg cgacccacac cgtgaaaatc ctcattttag gcaacggaca ggtaggttct
     4021 accgtcgcac aaaaccttgc cgccataccc aacaacgacg taaccgttat cgacatcgac
     4081 gaaaaagcat tgcaggaaac aggcagccgc ctcgatgtcc aaaccgtttt cggcaacggc
     4141 gcatccccct tcacattaga acgcgccggc gcggaagatg ccgacttgct gctcgcgctc
     4201 tcccgcagcg acgaaaccaa catcgtcgcc tgcaaagttg ccgccgacct gttcaacatc
     4261 cccggccgca tcgcgcgcgt ccgttccagc gaatacctcg aatacctcag ccccaagctc
     4321 gaaaacaacg aaaacggcag cctttccata ttcggcataa ccgaaaccat cagccccgaa
     4381 cagctcgtta ccgaacagct tgccggcctg atagactgcc cgggcgcatt gcaggtttta
     4441 cgttttgcag acgaccgcgt gcggatggtc atcatacagg cgcggcgcgg cggactgctt
     4501 gtcggacgca gcattgccga catcgcccaa gatttgcccg acggggccga ctgccaaatc
     4561 tgcgccgttt accgcaacaa ccgcctcatc gtccccgcgc cgcaaaccgt catcatcgaa
     4621 ggcgacgaaa tcctgtttgc cgccgccgcc gaaaacatcg gcgcggtcat acccgaattg
     4681 cgccccaaag aaaccagcac ccgccgcatc atgattgccg gcggcggcaa catcggctac
     4741 cgtctcgcca agcagctcga acacgcatac aacgtcaaaa tcatcgaatg ccggccgcgc
     4801 cgtgccgaat ggatagccga aaacctcgac aacaccctcg tcctgcaagg ttcggcaacc
     4861 gacgaaaccc tgctcgacaa cgaatacatc gacgaaatcg acgtattctg cgccctgacc
     4921 aacgacgacg aaagcaacat tatgtccgcc cttttggcga aaaacctcgg cgcgaagcgc
     4981 gtcatcggca tcgtcaaccg ctcaagctac gtcgatttgc tcgaaggcaa caaaatcgac
     5041 atcgtcgtct ccccccacct catcaccatc ggctcgatac tcgcccacat ccggcgcggc
     5101 gacatcgttg ccgtccaccc catccggcgc ggcacggcgg aagccatcga agtcgtcgca
     5161 cacggcgaca aaaaaacttc cgccatcatc ggcaggcgca tcagcggcat caaatggccc
     5221 gaaggctgcc acattgccgc cgtcgtccgc gccggaaccg gcgaagtcat tatgggacac
     5281 cataccgaaa ccgtcatcca agacggcgac cacatcatct ttttcgtctc gcgccggcgc
     5341 atcctgaacg aactggaaaa actcatccaa gtcaaaatgg gctttttcgg ataaaccgcc
     5401 ccattccgga catattgccg ccaagcggta tggaagcgga aataatggta ggtgggcttc
     5461 agacggcatc cgccctcccc gtcattcccg cgtaagcggg aatccagacc ttgggatagc
     5521 ggcaatattc aaaggttata aaagacccgt cattcccgcg caggcgggaa tccagacctt
     5581 gggatagcgg caatattcaa aggttatctg aaaatttaga ggttctagat tcccgctttc
     5641 gcgggaatga cgaaaagttg cgggaatcca gaacgtcggg caacggcaat attcaaaa
//
LOCUS       DAXHSJ010000128         5667 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000128 DAXHSJ010000000
VERSION     DAXHSJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.128"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            74..1000
                     /gene="fabD"
                     /locus_tag="ACFM8U_001645"
     CDS             74..1000
                     /gene="fabD"
                     /locus_tag="ACFM8U_001645"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC6334854.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFTDAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICTESAQGEVVEAVNFN
                     SPGQVVIAGNAAAVERAMTAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIETH"
     gene            1139..3004
                     /gene="msbA"
                     /locus_tag="ACFM8U_001646"
     CDS             1139..3004
                     /gene="msbA"
                     /locus_tag="ACFM8U_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC6334855.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGILSTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVIIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLALQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADCIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            3104..4669
                     /gene="guaA"
                     /locus_tag="ACFM8U_001647"
     CDS             3104..4669
                     /gene="guaA"
                     /locus_tag="ACFM8U_001647"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC6334856.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLEEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVSPGNQRE
                     FGYAQVKTIDSELTRGIQDDAPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     AEKRFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPSWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAKEKKLTNAKWLAQGTIYPDVIES
                     AGAKTQKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            4731..5477
                     /gene="fabG"
                     /locus_tag="ACFM8U_001648"
     CDS             4731..5477
                     /gene="fabG"
                     /locus_tag="ACFM8U_001648"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC6334857.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQAFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         1372 a         1821 c         1384 g         1090 t
ORIGIN      
        1 tatttcgttc cgcccgaact ttttgtaaaa ctcgggcaga atacctgatt atccaaccaa
       61 ataaaggaat accatgtctt ttgccttctt ttttcccgga caaggttccc aaagcctcgg
      121 tatgatgaac ggctttgccg aacacgccat cgtcaaaaac acatttgccg aagcctccgc
      181 catattgggg caggacttgt gggcgatgat taacggcagc gatgccgaaa tcatcggtca
      241 aaccgtcaac acccagccca ttatgctcgc cgccggcgtt gccgtttacc gcgcctattt
      301 ggaagcgggc ggcaaaacgc ctgccgccgt tgccggacac agcctcggcg aatacaccgc
      361 actcgttgcc gccggcgcat tggattttac cgatgccgtc aaactcgtgc gcctgcgcgc
      421 cgaactgatg cagtccgccg taccgcaagg cgtgggcgca atggcggcga ttctcggctt
      481 ggaagatgaa caggtcaggc agatttgcac cgaatccgca cagggcgaag tggtcgaagc
      541 cgtcaacttc aactcgcccg gacaagtcgt gattgcaggc aatgccgccg ccgtcgagcg
      601 cgccatgacc gccgccaaag aagccggtgc caaacgcgcc ctgccgctgc ccgtgtccgt
      661 accttcccat tgcagcctga tgaaacccgc cgccgacaaa cttgccgaag ccctgaaaac
      721 cgttgaaatc aagcagccgc aaatccgcgt catccacaat gctgacgttg ccgcctacga
      781 tgatgccgac aaaatcaaag acgcgctcgt ccgccagctt tacagccccg tacgctggac
      841 agaaaccgtc aacgcccttg tttcagacgg cattgccgaa tccgccgaat gcggcccggg
      901 caaagttttg gcgggcttgg caaaacgcat caacaaagcc gccgcgtgca gcgcgctgac
      961 cgatgccgga caggttgccg cctttatcga aacgcactga cttcgttccg caaaaagcag
     1021 cctgcatgcc tcaggctgct tttcacgtcc gaacggcaaa cagccgcata tttgcgctat
     1081 aattgatgac gaccaaacca ccgacagcgg ctgccgttgc agttcccgcc ctaccgatat
     1141 gatagaaaaa ctgactttcg gactgtttaa aaaagaagac gcgcgcagct ttatgcgcct
     1201 gatggcatac gtccgcccct acaaaatccg catcgttgcc gccctgattg ccattttcgg
     1261 cgttgccgcc accgaaagct accttgccgc cttcatcgcc cccctgatta accacggctt
     1321 ttccgcaccc gccgcgccgc ccgagctgtc tgccgccgcc ggcatccttt ccaccctgca
     1381 aaactggcgc gaacagttta cctatatggt ttgggggacg gaaaacaaaa tctggaccgt
     1441 cccgctcttc ctcatcatcc tcgtcatcat ccggggcatc tgccgcttta ccagcaccta
     1501 tctgatgact tgggtctccg tgatgaccat cagcaaaatc cgcaaagata tgtttgccaa
     1561 aatgctgacc ctttcctccc gctaccatca ggaaacgccg tccggcaccg tactgatgaa
     1621 tatgctcaac ctgaccgaac agtcggtcag caacgccagc gacatcttca ccgtcctcac
     1681 gcgcgacacg atgatcgtta ccggcctgac catcgtcctg ctttacctca actggcagct
     1741 cagcctcatc gtcgtcctga tgttccccct gctctccctg ctctcgcgct actaccgcga
     1801 ccgtctgaaa cacgtcattt ccgactcgca aaaaagcata ggcacgatga acaacgtgat
     1861 tgccgaaacc catcagggac accgcgtcgt caagctgttc aacgggcagg cgcaggcggc
     1921 aaaccggttc gacgcggtca accgcaccat cgtccgcctc agcaaaaaaa tcacgcaggc
     1981 aacggcggcg cattccccgt tcagcgaact gattgcctcg atcgccctcg ccgtcgtcat
     2041 cttcatcgcc ctgtggcaaa gccaaaacgg ctacaccacc atcggcgaat ttatggcatt
     2101 catcgtcgcc atgctgcaaa tgtacgcccc catcaaaagc cttgccaaca tcagcatccc
     2161 tatgcagacg atgttcctcg ccgccgacgg tgtatgtgca tttctcgaca ccccgcccga
     2221 acaggacaaa ggcacactcg cgctgcagcg tgtcgaaggg cgcatcagct tccgcaacgt
     2281 cgatgtcgaa taccgttcgg acggcatcaa agccctcgac aacttcaacc tcgacatcag
     2341 acaaggcgaa cgcgtcgccc tggtcggacg ttccggcagc ggcaaatcca ccgtcgtcaa
     2401 cctgctgccc cgctttgtcg aaccgtctgc cggcaacatc tgcatagacg gtatcgacat
     2461 cgccgacatc aaactcgact gcctgcgcgc ccaattcgcc ctcgtctccc aagacgtatt
     2521 cctgtttgac gacaccctgt ttgaaaacgt ccgatacagc cgtcccgacg cgggcgaagc
     2581 cgaagtcctg tccgccctcc aagccgccaa cctgcaaagc ctgattgaca gttccccgct
     2641 cgggctgcac cagcccatcg gatcgaacgg cagcaacttg tccggcggac agcggcaacg
     2701 cgtcgctatt gcccgcgcca ttttgaaaga cgcgccgata ttattattgg acgaagccac
     2761 cagcgcattg gacaacgaat ccgaacgcct cgtccaacaa gcgctcgaac gcctgatgga
     2821 aaaccgcacc ggcatcatcg tcgcccaccg cctgaccacc atcgaagggg ccgactgcat
     2881 catcgtaatg gacgacggca aaatcatcga acaaggcaca cacgaacaac tgatgtccca
     2941 aaacggctac tacacgatgt tacgcaatat ctcaaacaaa gatgccgccg tccggacggc
     3001 ataaacaaaa tgccgtctga aatggtacaa tcgccccgac cctttcagac ggcatcatat
     3061 ccgccgaccc atccgattat cttcaatcac tgtaaaaccc attatgaccc aagacaaaat
     3121 cctcatcctt gacttcggtt cgcaagttac ccagctcatc gcccgccgcg tgcgcgaagc
     3181 ccacgtttac tgcgagctgc attccttcga tatgcctttg gaagaaatca aagccttcaa
     3241 tccgaaaggc atcatccttt ccggcggccc caattccgtt tacgaatccg actatcaagc
     3301 cgataccggc atttttgatt tgggcattcc agttttgggc atctgctacg gaatgcagtt
     3361 tatggcgcac cacttgggcg gcgaagttag cccgggcaac caacgcgaat tcggttatgc
     3421 gcaagtcaaa accatcgaca gcgagctgac acgcggcatt caagatgatg cgccaaacac
     3481 gctcgacgta tggatgagcc acggcgacaa agtgtccaaa ctgcccgacg gtttcgccgt
     3541 catcggcgac accccgtcct gcccgattgc catgatggaa aacgccgaaa aacgattcta
     3601 cggcatccag ttccaccccg aagttaccca caccaaacaa ggccgcgccc tgttgaaccg
     3661 ctttgtcttg gatatttgcg gcgcacaacc gagctggacg atgccgaact acatcgaaga
     3721 agccgttgcc aaaatccgcg aacaagtcgg cagcgacgaa gtgattttag gtttgtcagg
     3781 cggcgtagac tcttccgtag ccgccgcgct gattcaccgc gccatcggcg accaactgac
     3841 ctgcgtgttc gtcgatcacg gtttgttgcg cctgaacgaa ggcaaaatgg tgatggatat
     3901 gttcgcccgc aacttgggtg tgaaagtgat tcacgtcgat gccgaagggc agtttatggc
     3961 gaaactcgcc ggcgttaccg accctgagaa aaaacgcaaa atcatcggcg cggaatttat
     4021 cgaagtattt gatgccaaag agaaaaaact caccaacgcc aaatggctgg cgcaaggcac
     4081 gatttacccc gacgtaatcg aatccgcagg cgcgaaaacc caaaaagccc acgccatcaa
     4141 atcgcaccac aacgtcggcg gtctgcccga aaatatgaag ctcaagctgc ttgagccgtt
     4201 gcgcgacttg tttaaagacg aagtgcgcga actgggcgtt gctttgggtc tgccgcgcga
     4261 aatggtgtac cgccatccgt tccccggccc cggtttgggc gtgcgcatct tgggcgaagt
     4321 gaaaaaagaa tacgccgact tattgcgtca agcagacgat attttcatcc aagaattacg
     4381 caacaccacc gacgaaaacg gcacatcttg gtacgacctg accagccagg catttgccgt
     4441 attcctgcct gtgaaatccg tcggcgtaat gggcgacgga cgcacttacg actacgtcgt
     4501 cgcactgcgc gcagtcatca ccagcgactt catgaccgca cactgggcag aactgcctta
     4561 ctcactgctc ggacgcgtgt ccaaccgcat catcaacgaa gtcaaaggca tcaaccgcgt
     4621 ggtgtacgat gtcagcggca aaccgcctgc caccatcgag tgggaataac cagcaaacag
     4681 ggctgcaccg tccggcgcag tctttcgatt atcggaaaaa aggaaaaaat atgagcacac
     4741 aagatttaag cggcaaaatc gctttggtaa ccggcgcatc gcgcggtatc ggtgcagcaa
     4801 ttgccgacac actggcggca gccggtgcca aagtcatcgg tacggcgacc agtgagagcg
     4861 gtgcggcggc gattagcgag cggttggcgc aatggggcgg cgaaggccgc gtattaaatt
     4921 ccgccgaacc cgaaaccgtc gaaaacctga ttgccgacat cgaaaaaacg ttcggcaaac
     4981 tcgatattct ggtcaacaac gccggcatca cccgcgacaa cctcctgatg cgcatgaaag
     5041 aagaagagtg ggacgacatc atgcaggtca acctcaaatc cgtgttccgc gcttctaaag
     5101 ccgtcttgcg cggcatgatg aagcagcgcg ccggccgtat catcaacatc acatccgtcg
     5161 tcggcgtgat gggcaatgcc ggtcaaacca actatgccgc ggcaaaagca ggcttaatcg
     5221 gtttctccaa atccatggcg cgcgaagtcg gcagccgggg cattaccgtc aactgcgtcg
     5281 cccccggctt tatcgatacc gatatgaccc gcgccctgcc ggaagaaacc cgccaagcct
     5341 ttaccgccca aaccgccttg ggcagattcg gcgacgcgca agacattgcc gatgcggttt
     5401 tgttcctcgc ttccgatcag gcgaaataca ttaccggcca gacgctgcac gtcaacggcg
     5461 gcatgctgat gccctgacgg acagccgtgc cgacaatgcc gtctgaagcc ctttcagacg
     5521 gcatttgcat tcccaaacaa aatgaacaca caccacaccc tgccctgccc ctgcggttca
     5581 ggcacaagct gagacctttg caaaactcct ttccctcccg acagccgaaa cccaaacaca
     5641 ggttttcgtc tattttcgcc ccaaata
//
LOCUS       DAXHSJ010000129         5658 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000129 DAXHSJ010000000
VERSION     DAXHSJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.129"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            98..814
                     /locus_tag="ACFM8U_001649"
     CDS             98..814
                     /locus_tag="ACFM8U_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC6334858.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            963..1865
                     /locus_tag="ACFM8U_001650"
     CDS             963..1865
                     /locus_tag="ACFM8U_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC6334859.1"
                     /translation="MDSLMTLLSVLVPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            1908..2681
                     /locus_tag="ACFM8U_001651"
     CDS             1908..2681
                     /locus_tag="ACFM8U_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC6334860.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEAGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2793..4481
                     /locus_tag="ACFM8U_001652"
     CDS             2793..4481
                     /locus_tag="ACFM8U_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC6334861.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVLELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4574..5530
                     /gene="gshB"
                     /locus_tag="ACFM8U_001653"
     CDS             4574..5530
                     /gene="gshB"
                     /locus_tag="ACFM8U_001653"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC6334862.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELSERDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1309 a         1545 c         1567 g         1237 t
ORIGIN      
        1 aatttgtgca atgctttaat tcctctgaca cgatttgaaa cacagccgcc ttaaaacatc
       61 ttaaaaaatt tgaaagcgta aaaatacgga aaacatcatg aactttgaaa acgacgacat
      121 tatccatgcg ccgaccacgt cttccctgat tctcgaagag cggcacgatt cggagctgtt
      181 ccgtgtttac gccctgattt tggacggcat taccgatcag gtgctgctgc ccggcaaaaa
      241 gctgaccgaa tccgaacttt gccgtcagat ggtgtgttcg cgcaacaccg tccgcggcgc
      301 gctgtcgctt ttggcgcacg acaagattgt cgatttgcaa cccaacaggg gcgcgttcgt
      361 ccacgttccc gatttgaaag aaatgcagga tgtgttcaat gcgcgcatcg aaatggagac
      421 gatgattttg aatatcctcg cagatttgcc ggatttggaa acgcgcctca agccgcttta
      481 tgcgatgata aggcgcgaag aagaggcttc cggcaggggc gaccgcgtcg gctggaaccg
      541 cctgtccaat gccttccacg tcgaactggc gcgcctggtg ggcaacgatg tgctgttcga
      601 cattatgaac acgctgtgcg cgcggtcttc cctgattgtc gccgtggcgg gcgtgcatcg
      661 cgaggaaaaa cacgccatca atacgcatac gcattccgaa caccgcgaaa tcctcgacct
      721 gctgctggcg ggcaggcgca accgcgtggt caaaatcctg cgccgccatt tgggcaactg
      781 tatggaacgc ttggaaaaga ctttggaaga ttgaatgccg gagcggataa gccgcctgaa
      841 ccttcaggcg gttttttaat ggcgaacctg atgccgtctg aaatatggat gcgggtatct
      901 gcaattttca gacggcaatt tttaaaccgc atatatcatg cggcaataaa ggaggtcagg
      961 agatggacag cctgatgaca ttgctttcgg tattggtacc gatgttcgcc ggatttttta
     1021 tccgtgtgcc caagccttac ctgcccgctt tggacaaggt gctgtcggtt ttggtgtatg
     1081 ccgtgctgct gctgatcggc gtatcgttgt cgcgcgtgga ggatttgggt tcgcggttgg
     1141 acgatatggc gttgacggtt ctgtggctgt ttgtttgtac ggtcggggcg aacctgcttg
     1201 ctttggcagt gttgggaaag ttattcccgt ggcggataaa ggggaaaggg aagggcgttt
     1261 cggtcggcgt gtcgggcagt gtggggcagc tcggatgcgt gctgctcgga tttgcattcg
     1321 gcaaactgat gcgcgatatt tggatgccgt ctgaaagcgc gggcatgtat tgtctgatgc
     1381 tgctggtgtt cctcatcggc gtacagctca aaagcagcgg cgtatcgttg cggcaggttt
     1441 tggtcaaccg caggggtatt cggttgtcgg tctggtttat gctttcatct ctttcgggcg
     1501 ggctgctgtt tgccgcatcg acagacggtg tgtcgtggac gaaaggtttg gcgatggctt
     1561 ccggcttcgg ttggtattcc ctctcgggtt tggtcatgac cgaggcttac ggcgcggtat
     1621 ggggcagcat catgctgctg aacgatttgg cacgagagct gtttgcactg gcatttatcc
     1681 cgctgctgat gaagcgtttt ccggatgcgg cggtgggggt tggcggtgcg accagtatgg
     1741 attttacatt gcccgtgatt cagggtgcgg gcggtttgga agtcgtgccg gtagcggtca
     1801 gcttcggcgt ggtggtcaat attgccgccc cgtttctgat ggtggtgttt tccacgctgg
     1861 gctgaacacg gtaaaatcgg catcccgatg caaggaggca gaaaacgatg aaaccgaaaa
     1921 tccaaaggca cggagagatt ttaagccttg tccgccggca tcagtttatg tcggtggacg
     1981 agcttgccgc cgcattggac gttaccccgc agacgatacg ccgcgacatc cgcgagttgg
     2041 aggaggccgg cagcctgaaa cgccatcacg gcggcgcatc ttcgggcgga aacttgccgg
     2101 agggcctgcc cgccgaccgc caaacccggt gtcaaaacga aaaaaacgcc attgcccggc
     2161 tgattgcgga acacatcccc gacggttcgt cgctgtttgt cagtatcggc acgacgatgg
     2221 aggccgtggt gtcggagctg gtcaggcggc gcggcagcct gagggtgatt accaacaata
     2281 tccacgccgc ctcgattgct tcggcgcgga cggattatac ggtcattatc acttctggag
     2341 tcgtccgtcc tttggacggc ggtattaccg gcgtggcaac cgtcgatttt atcaaccagt
     2401 tcaaagtcga ttatgccgtg atgagtacgc acggcgtgga aagcgacggt tcgcttttgg
     2461 acgacgacta caaagaagtc agcgtgatgc aggcgatgat tgccaacgcg cgcgtccgct
     2521 tcctcggcgt ggatcacagc aaattccgca gcaacgcgct ggtcaggctt ggcgacatta
     2581 cggcgtttga caaagtattt accgaccggc tgcccgatac cgcgatgcag aagatgctga
     2641 aagaggcggg ggtggaatgc ctgattgccg atgccgtctg aacgctatgt caaagcgcgc
     2701 aagtcgggta caataaacac atcatcaaac cgcttcagac ggcataagga accgccccaa
     2761 tgccgtctga agccatctgt tcaaagaaaa ctatgctcaa taaagaccaa tttgcggaca
     2821 accattttat ccgcaccttc atcgaagaag acctcaaaag cggcaaacac gaaaccatcc
     2881 aaacccgttt tccgccggag cccaacggct acctgcacat cggacacgcc aaatccatct
     2941 gcctgaactt cggtttggcg tatatttacg acggcttgtg caacctgcgt tttgacgata
     3001 ccaaccccga aaaagaaaac gacgaatacg tcaacgccat caaagaagac gttgagtggc
     3061 tcggtttcca ttgggcgggc gagccgcgtt tcgcttccaa ctatttcgac cagctttatg
     3121 actacgccgt cggtttggtt aaagacggca aagcgtatgt cgatgatttg acgccagaag
     3181 aaatgcgcga ataccgcggt acgctgaccg aagcaggcaa aaacagccct tatcgcgacc
     3241 gcagtatcga agaaaacctc gacctgttca cgcgcatgaa aaacggtgag ttccccgacg
     3301 gcagcaaaac cctgcgcctg aaaatcgaca tggcatccgg caacatcaat atgcgcgatc
     3361 cggtcatcta ccgcatccgc cgcgcccatc accacaacac cggcgacaaa tggtgcatct
     3421 atccgatgta cgactacacg cattgtattt ccgatgccat cgaaggcatc acgcattcct
     3481 tgtgtacgct tgagtttgaa gcccatcgtc cgctgtatga ctgggtgttg gacaacatcc
     3541 ccgcgccgca cgccacacgt ccgcgccaat acgagttttc acgcttggag cttttgtaca
     3601 ccattacctc caaacggaaa ttgaatcagt tggttgtgga aaaacacgtt tccggctggg
     3661 acgatccgcg tatgccgacc atttccggta tgcgccgacg cggctacacg cccgaagggc
     3721 tgcgcctgtt tgccaaacgc gccggtattt ccaaatctga aaacatcgtc gatatgagcg
     3781 tgttggaagg cgcgattcgc gaagagttgg aaaattccgc cccacgcctg atggcggtgt
     3841 tgaacccgct caaagtaacc ctgaccaact ttgaaaccgg cagaacccaa agccgccgtg
     3901 ccgcgttcca tccgaaccac gaagaaatgg gcgatcgcga agtacctgtt tcacaaacca
     3961 tttacatcga agccgacgac tttgccgaaa atccgcccaa aggcttcaaa cgcctgattc
     4021 ccggcggcga agtacgcttg cgccacggct atgtcatcaa gtgcgatgaa gtcgtcaaag
     4081 acgaagcagg caatgtgctt gaactcaaat gcagcatcga ccacgacacc ttgggcaaaa
     4141 atccagaagg ccgtaaagtt aaaggcgtga ttcattgggt ttccgccgaa cacgccgccg
     4201 aaatcaaagt ccgtctgtac gaccgcctct ttaccgtcga gcgtcccgat gccgtgcgcg
     4261 gcgaagacgg cgaatacctg ccgttcaccg atttcctcaa tccggaatcc gtcaaggaaa
     4321 tcaccgccta cgccgaacct gccgccaaag atttgccggc agaaagccgt tggcagttcg
     4381 agcgtatcgg ctacttcgta accgaccgta aagaccacgg caaagacacg ccggtgttta
     4441 accgcacggt cacgctgaaa gattcttggc agcctaagta aaaccccatc cttgccgtct
     4501 gaatattgtt cggacggcat ttcccctttg cccgcgaaat gcggcacatt cggcacaccg
     4561 gaaaggaaat atgatgaaag tcctctttat cgccgacccg atggcaagtt tcaaaaccta
     4621 caaagacacc acctacgcca tgatgcgcga gatggcaaaa cgcggctggc ggctgtttca
     4681 taccttgagc agcggattgt cggttaaaaa cggtctggtg gttgctgatg cgtctgcttt
     4741 tgagtttctc ggcgcacaag acgaccacga caaaaactgg tttgccgccg ccgacaaagt
     4801 tcagacggca ttgaaagcat ttgatgccgt gattatgcgt accgatccgc cgttcgatat
     4861 gcaatacctc tacgccacac agcttctgac gctggcggag cagcagggcg cgaaggtctt
     4921 taacagcgga cagtcgatgc gtgacttcaa tgaaaaactg gcgattttga acttcagccg
     4981 ctttaccgcg cccacgctgg taacgacccg ttccgccgat gtccgcgcat ttttgaaaga
     5041 acacggcgac atcatcatta aaccgctcga cggcatgggc ggtatgggca tcttccgcct
     5101 gaccgaaaaa gaccccaaca tcggcagcat cctcgaaacc ctgatgcagc ttgattcccg
     5161 caccattatg gcgcaacgct acattcccga aatcgtacac ggtgacaaac gcatcctgat
     5221 tatcggcggc gaagtcgtcc cctatgcttt ggcgcgtatt cctcaaaacg gcgaaacacg
     5281 cggcaacctt gcggcaggcg ggcgcggcgt ggcgcaggaa ttgagcgaac gcgaccgcga
     5341 aattgccgaa acgctcgccc ccgaactcaa acggcgcggc atcctgctgg ccggtttgga
     5401 cgttatcggc agcaacctga ccgaagtcaa cgtaaccagc ccgaccggat tccaagaaat
     5461 tatgaaacaa aaaggcttcg atgtggcggc aatgtttgcc gatgccgttg ccgcgtggtc
     5521 ggtacgttaa accgatgccg tctgaaaggc ttttgcttca caaccgcctg gtttgatcgg
     5581 gcaggcaggg ttttccctca gtcggcagag atacgcttaa taccgtacgg acaccccgcc
     5641 gccccgtttt cagacggc
//
LOCUS       DAXHSJ010000130         5625 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000130 DAXHSJ010000000
VERSION     DAXHSJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.130"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            114..635
                     /locus_tag="ACFM8U_001654"
     CDS             114..635
                     /locus_tag="ACFM8U_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
                     /protein_id="HFC6334863.1"
                     /translation="MSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAKI
                     QPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDAVNTRGYSSKPIDTLMALA
                     NDGTIAGAKLVDHHEPIMLIGIPHLPAPGRAIRLNWLPAYIKPNFISTNRLRLKGLPT
                     VLQPSKGTGAGVP"
     gene            524..1723
                     /locus_tag="ACFM8U_001655"
     CDS             524..1723
                     /locus_tag="ACFM8U_001655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HFC6334864.1"
                     /translation="MASGVYQTKLYIDKPITIEGPADRSATIEGDRSGRTIAVHAPDV
                     TLRNLTVTRSGMSLPAMDAGIYLEETAPRALIEHNNILDNSVGVYLHGSAEAMVRENK
                     IVGDATLRVNERGNGVTVWNAPGAQVVGNDISKGRDGIFSNTSTHNTYKNNRFSDLRF
                     AVHYMYTNDSEVSGNISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIH
                     DNIINKAGKCVFAYNANYDKLSANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKY
                     VSTRFLDWSEGGHGNYWSDNSAFDLNGDGFGDSAYRPNGIIDQVIWRAPVARLLMNSP
                     AISIVKWAQAQFPAVLPGGVVDSKPLMKPYAPKIQTRYQAMKDELLKEAETRQSEWGR
                     AGNGSLN"
     gene            1781..2395
                     /locus_tag="ACFM8U_001656"
                     /pseudo
     CDS             1781..2395
                     /locus_tag="ACFM8U_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2412..2543
                     /locus_tag="ACFM8U_001657"
                     /pseudo
     CDS             2412..2543
                     /locus_tag="ACFM8U_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2540..3034
                     /locus_tag="ACFM8U_001658"
     CDS             2540..3034
                     /locus_tag="ACFM8U_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC6334865.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(3122..4783)
                     /locus_tag="ACFM8U_001659"
     CDS             complement(3122..4783)
                     /locus_tag="ACFM8U_001659"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC6334866.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHKDGSVKGIATGNM
                     GVGKNGEPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVMHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGFQSLPKLSFKGGVLVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPADVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(4913..5462)
                     /locus_tag="ACFM8U_001660"
                     /pseudo
     CDS             complement(4913..5462)
                     /locus_tag="ACFM8U_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(5407..5580)
                     /locus_tag="ACFM8U_001661"
                     /pseudo
     CDS             complement(5407..5580)
                     /locus_tag="ACFM8U_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1322 a         1532 c         1439 g         1332 t
ORIGIN      
        1 tcccgtatgc ggattggttt taagatttgt aaatttgatt tgcatcaaaa aatcgccgat
       61 agatgattca tataatatca atattaaaga gtatcggtat atcggggata gtcatgtcct
      121 gtttttcaat caaacgtatg tccgcgtttc gggcgcggat aacagcgttt tttgccgcct
      181 ttgtcttttt gacggcggca ctgcccgctt atgcggagcg tctgcctgat tttctggcga
      241 aaatacagcc ttcggaaatt tttccgggtg cggaccgtta cggcaagccg gaaggtaagc
      301 ctatggttgc ccgcgtttac aaaggcgatg agcagttggg cttggtctat atcacgaccg
      361 atgcggtcaa tacgcgcggt tattcgagca aaccgatcga tacgctgatg gctttggcaa
      421 atgacggcac gatagccgga gcgaaactgg tcgatcatca cgaaccgatt atgctgatcg
      481 gtatcccgca tttgcccgcg cccgggcggg cgatacgctt aaactggctt ccggcgtata
      541 tcaaaccaaa ctttatatcg acaaaccgat tacgattgaa gggcctgccg accgttctgc
      601 aaccatcgaa ggggacagga gcgggcgtac catagccgta cacgcgccgg acgtaacgct
      661 ccgcaacctg accgttaccc gttccggtat gagcctgcca gcaatggatg ctggtattta
      721 tctcgaagaa accgccccgc gcgccctgat tgaacacaac aatattttgg ataattcggt
      781 cggcgtctat ctgcatggtt ctgccgaagc gatggtacgc gagaataaaa tcgtcggcga
      841 tgcgactttg cgcgtgaacg agcgcggcaa cggcgttacc gtttggaacg cgcccggcgc
      901 gcaggtcgtc ggcaacgaca tttccaaagg gcgggacggc attttttcca acaccagcac
      961 gcacaacacc tataaaaaca accgcttcag cgatttgcgt ttcgccgtcc actatatgta
     1021 caccaacgac agcgaagtca gcggcaatat ttccgtgggc aacaatatgg gctatgtgct
     1081 gatgttttcc gagcggctca aagtgtttga caatatcgcc gtcggcagcc gcgatcaggg
     1141 cattatgctc aactatgtca actattccga tattcacgac aacattatca acaaggcggg
     1201 caagtgcgtt tttgcctaca atgccaacta cgataaactg tccgccaatc attttgaaaa
     1261 ctgccaaatc ggcatacact ttaccgccgc catcgaaggc acgtccctgc atgacaattc
     1321 ctttatcaac aacgaaagcc aggtcaaata cgtcagcacg cgctttctcg actggagcga
     1381 gggcggacac ggcaactatt ggagcgacaa cagcgcgttc gatttgaacg gcgacggctt
     1441 cggagacagc gcgtaccgtc ccaacggcat tatcgaccaa gtcatctggc gcgcaccggt
     1501 tgcacgcctt ctgatgaaca gtcccgcaat cagcatcgtc aaatgggcgc aggcgcagtt
     1561 tcccgccgtt ttgcccggag gcgtggtgga cagcaaaccg ctgatgaagc cttatgcccc
     1621 caaaattcaa acccgttatc aggcgatgaa ggacgagctg ctcaaagaag ccgaaacgcg
     1681 gcagtcggaa tggggcaggg cgggaaacgg ttctttgaac tagtctgctt cagacggcat
     1741 ccggattcaa atgccgtctg aaaacacaaa aggaacaacc atgaccacac atcatgtcga
     1801 attgaggaag gtaaccaaac gcttcggcgc gcaaaaagcc gtcaaccaag tcgatttggt
     1861 tttgaaggca ggagaaagcg tcgggcttgc cggacacaac ggtgcgggca agtccaccat
     1921 tatgaagctg atactcgggc tgattacccc gaccgaaggc gaagtgatgc ttttgggcga
     1981 acgtaccggc agcaaagcgg gggcgcggct tcgcagccaa atcggctacc tgcccgaaac
     2041 tgttgcgttg cacccttcgc tgaccggcat cgaaacgctg gatttttatg ccaaacttaa
     2101 aaaacagccg ctcacgcaga atcgggggct gctcgagcgc gtcggcattt cacaggcggc
     2161 acaccgccgc gtcggcactt attctaaagg gatgcgccaa cgccttgcct tggcacaagc
     2221 cctgctgggc gagcccaaag tcctgctgtt tgacgaaccg acaaccggcc tcgaccctgc
     2281 atcaaggcaa atgttttacg aagtcgtgcg cgaactcaac gggcgcggcg cgaccgtatt
     2341 gctcagtacc cacgcccttg ccgagttgga cgggcacgcc gaccgcatta tcgtggatta
     2401 aatttaatcc actatatgcg ggtatggcgg gtttgagcgg acaaatcagc ctgaccgtcc
     2461 ccgttttgct gaccgctcag gttttatggg ttatcattcc gcttgttttg gcagccggaa
     2521 tttttagaaa gcgacaaata tgaaaaaaac cctgttggca attgttgccg tttccgcctt
     2581 aagtgcctgc cggcaggcgg aagagggacc gccgccttta ccccggcaga ttagcgaccg
     2641 ttcggtcgga cactattgca gtatgaacct gaccgaacac aacggcccca aagcccagat
     2701 tttcttgaac ggcaaacccg atcagcccgt ttggttctcc accatcaagc agatgttcgg
     2761 ctataccaag ctgcccgaag agcctaaagg catccgcgtg atttacgtta ccgatatggg
     2821 caatgttacc gattggacga atcccaatgc cgacacggag tggatggatg cgaaaaaagc
     2881 cttttacgtc atcgacagcg gctttatcgg cggtatgggt gcggaagacg cgctgccgtt
     2941 cggcaacaaa gagcaggctg agaaatttgc aaaggataaa ggcggtaagg ttgtcggttt
     3001 cgacgatatg cctgatacct atattttcaa ataatattga aaaaaccggc cgaaacttta
     3061 aaaacgagtt tcggtcggtt tttcttttct tgttcggtat agtgtcggca ggaaagaacc
     3121 ttcacatccc gccgtaattc ggcccgctcg cgccttcggg gcaaatccaa gtgatgtttt
     3181 gcgtcgggtc tttgatatcg caggttttgc agtgcacgca gtttgccgcg ttgatttgca
     3241 ggcgcggatt gccgttttct tcaacaattt cgtacacgtc ggccggacaa tagcgcgttt
     3301 cgggcgaggc gtattctttg tagttcacgt ctatcatcgt ttgcggattg ttcagcacca
     3361 aatggtcggg ctggttttct tcgtgcgcga gattggcaag gaagacgctg ctcaagcggt
     3421 cgaaggtcaa cacgccgtcg ggtttcggat aatcaatcgg cttgcacgcg gcggcttttt
     3481 tgagctgctc gttgtctttg ccgtgatgtt tcaaggtcca cggggctttg cctctgaaaa
     3541 tcatctgatc gatgccggtg tagattgagc cgaggtaaac gccccatttg aatgacggac
     3601 ggacattgcg cgcggcgtaa agctcttgat acagccagct ttgttcaaaa cgttgctgat
     3661 aatccgccgc ctctttgccg ctgtcgaaac cctccacttc ttcaaggttt tccaacaagg
     3721 ggaacacggc ttcggcggcg agcatggcgg atttcatcgc ggtatgaatg cctttgatgc
     3781 gcggcatatt gaggaaaccc gccgcatcgc cgaccaaaac gccgcctttg aacgagagct
     3841 tcggcaaact ttgaaaaccg ccttcaatca gcgaacgcgc accgtaagca atgcggcggc
     3901 cgccttcaaa ggttttgcgg atttcgggat gggttttgaa acgttggaac tcttcaaacg
     3961 gcgacagata aggattttga tagtccaaac cgaccacgaa gccgacggca actttgttgt
     4021 cgtcgaaatg gtaaacaaac gcgccgccgt aggttttgct gtccagcggc cagcctgcgc
     4081 tgtgcatcac caaaccgggc tgatgctgtt cggacggcac ttcccaaact tctttaatgc
     4141 ccaagccgta agtttgcggc tggctgtttt ggtcgagttt gaagcgttcg atgatttgtt
     4201 tggaaagcga acctcggcag ccctcggcaa acagggtttg ccgtgcccaa agctccatgc
     4261 cgggctggaa tgaatcggtc ggctcgccgt ttttgcccac gcccatattg ccggttgcaa
     4321 tgcctttgac cgaaccgtct ttgtgataca gcacttcggc agcggcaaag cccggataaa
     4381 tttccacgcc caaattttcc gcctgctccg ccaaccagcg cacgacttcg cccaagctga
     4441 cgatgtagtt gccgtgattg tcgaaattgg gggtaatggg cagattgaac gcttttttct
     4501 ccgtcaggaa caacaccttg tcctgcgtta ccgtgcgcgt cagcggcgcg cctttttctt
     4561 tccagtcggg aatcaactca ttcagcgcaa tcgggtcaat gaccgcgccc gccagcgaat
     4621 gcgcgcccgc ctccgaacct ttctccacca cgcaaacgct gatttcgcgc ccgttctgtt
     4681 cggcaagctg cttgagtttg atggcggcgg acaaacccga tgggcctgcg ccgacaatca
     4741 cgacatcgta ttgcatactg tcgcgggtga tggattctgt catggcggtt cctgtgtatt
     4801 tattattgaa ttgcaaatcc gtaattatac aacgggaaca tatagttacc aaatacaaca
     4861 aaggtcgtct gaaaaccata ttttcggttt tcagacgacc tttgtcgaaa tttcaataag
     4921 cacgccacca ttttacctgt ccgaccgcaa actccgtctg acgtttcgga ctgcgtgtga
     4981 aaaacgcctt atccccgccg gcatccctcc ctttcggcac aaccgccaaa atcttacctg
     5041 ccaaatttcc ctcacgggtt tgccaagcat ccaaaaactg cgccctgctc atcgaaacat
     5101 gacccagcga cgggtcggca agcaaaaccg tattgccgtc tataccgcgc aataccgaga
     5161 aatgatcatc cttgcggtat ttcagataca cgatgacggg gatttgcaac tgtgcaagct
     5221 gctcgaaaga cagggcatag cctttcgcct caaaacccaa atcaggcatg atgcgcagca
     5281 tatcctcaaa cgacgcgcgc atctgctcct tatccagctt tttcaacacg tcctcttccg
     5341 tcagcttttg cccgtgaaaa ttgttcaaaa gccgtcgcca ccgaagccgc cccgcaggaa
     5401 aaatccaaat cctgctttac aatattgaaa tcgcgccttt ctttccaact ctgcactttg
     5461 atttttccat aagcaacagg attatagtgg attaaattta aaccagtacg gcgttgcctc
     5521 gccttgccgt actatctgta ctgtctgcgg tttcgtcgcc ttgtcctgat ttttgttaat
     5581 ccactatagg tttccgtgcg gacgtgttca gattcccgcc ttcgc
//
LOCUS       DAXHSJ010000131         5553 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000131 DAXHSJ010000000
VERSION     DAXHSJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.131"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(254..2050)
                     /locus_tag="ACFM8U_001662"
     CDS             complement(254..2050)
                     /locus_tag="ACFM8U_001662"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC6334867.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFVH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSYLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2508..3761
                     /locus_tag="ACFM8U_001663"
     CDS             2508..3761
                     /locus_tag="ACFM8U_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6334868.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCIISATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDKRHKLTAGMNF
                     LAIVGLLYLTGFAVLFLLNLSGLLA"
     gene            3916..5427
                     /gene="lysS"
                     /locus_tag="ACFM8U_001664"
     CDS             3916..5427
                     /gene="lysS"
                     /locus_tag="ACFM8U_001664"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC6334869.1"
                     /translation="MSEQNHPQIEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQAVPVKVAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASCTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
BASE COUNT         1211 a         1415 c         1609 g         1318 t
ORIGIN      
        1 cggaatatag cggattaaca aaaaccagta cggcgttgtc gcgccttagc tcaaagagaa
       61 cgattctctc aggtgctgaa gcaccgagtg aatcggttcc gtactattcg tactgtctgc
      121 ggcttcgtcg ccttgtcctg atttttgtta atccactata tttgcttgcg gcaagatgaa
      181 aacggtgcgg gatgttttgg gaaaccaatg ccgtctgaag ggctttcaga cggcattttt
      241 tgcgccgtgc tgtttaacgc gccagcggtt cggtgcgttt gatcagccac gcttttgccg
      301 cgccttcggt cagcggctcg aggcggcggc ggacttcggc gtggtagcgg ttgacccagt
      361 cgatttcgcc gtcggtcatg agggcggtgt ccatcaggcg ggtgtcgatg gggcagaggg
      421 tcagggtttc aaaacagagg aagctgccga attcggtttc ttgaggggcg gcgacggctt
      481 ggttggcggc aaggttttca atgcggatgc cccattttcc cgggcggtag agtccgggtt
      541 cgatggaggt aaccatgcct tttttcatgg cggtttcggg cgtggcgggg gcggcgaagg
      601 cgatgcgctg cgggccttcg tggacgttga ggaaatagcc tacgccgtgt ccggtgccgt
      661 ggccgtagtc gcattgcgcc tgccacaggg gtttgcggca aatcgcatca atcagcggcg
      721 aggggatgtt ttcggggaac acggcttcgg caagcgcgat atgggctttg agaacgaggg
      781 tgttgtcgcg tttttgttcg gcggtgggtg tgccgacagg gacgacgcgg gtgatgtcgg
      841 tcgtgccgcc tttgtattgc gcgccggagt cgatgagcaa aagcccgttg ccgctgatgg
      901 tgctgtggct ttcgggcgtt gcgctgtaat gcggcagtgc gccgttggcg ttgaagcctg
      961 cgatggtgtc gaaactcaat gaaatgaagc ctgggcgcac gctgcggtgg cgataaagca
     1021 tggtgtccac gtcgatttcg gtcaggctgc cgccgttgcc gatgatgtct tcaaactcgg
     1081 cgaagaaacc gcacaacgcc gcgccgtcgt gttccatcgc ttcgcggatg cgggcgatgt
     1141 cggcttcgga tttgcaggat ttgaacagcg tggacgggtt gattccctcg ataaggcgca
     1201 cgctttcggg caggcgcaca agcgtgctga cggcggtttt gttcggttcg ataagcaacg
     1261 cgccgccgat ttgcgcgagt ttgtcggcaa cttgggcgta aggctcgacc gtgatgcctg
     1321 cggtttgcag cgcggcggcg gcttcggcat tcagacggca tcggtcggta aacaggacgg
     1381 cgttgtcttt gccaatcaat agataggaca cgaaaacggg attgaaaggc acgtcgctgc
     1441 cgcgcaggtt ggtcagccag gcgatgtcgt cgagcgagga aaccaagtgg taatccgcgc
     1501 ctttttccgc catcacggcg cgcacgcggg cgagtttttc ggcggcggtt tcggagacgt
     1561 aggcggggtc gtggacgaac acggtttcgg cggggatagc gggacggctt gtccacactt
     1621 ggtcaagcag atcgtccggg tgttcgatgc ggatgttttt ggcggtcagg gattgcgcca
     1681 aagtgcgttt gccggtgagc gagaccatat cggaagggat gccgacggcg gcgttttcgg
     1741 gcaggtttgc cgcgagccat tcgttatacg gcggcacttg cccgcttttt tgcagttcga
     1801 tgccgctgcc cgcaagctgc ttggcggctt gttcccaata gcggctgtcc acccatacgc
     1861 ccgcttcatc ggtggtcagg acaaacgtgc cgaccgagcc ggtaaagccc gacaattcgc
     1921 gccgcgcctg ccaatgttcg ggcaggtatt cggacaggtg ggggtcggcg gaagggatga
     1981 cgagtgcatc caagccttgc gccttcatgg cttcgcgcaa tgcggacagg taattcgata
     2041 cggtattcat aacagttcct ccaatcgggt tttgcggctt cagacggcat ggacggaaat
     2101 ctgaaatgcc gtctgaaaaa cataaccatc ttatcaaatc gccagcctgc tgcaaaacgg
     2161 acgggctgat cggatggtgc gtgtttgcgc gaaaaaagcg atgtaaggtt gtttgttgta
     2221 aaattttagg cataaaatgt cgttttatgc ctttttttgg aagtaatatg gaaaatatag
     2281 tcaagtttaa gcatgaagtt ctaatagtgt gtgttggcag gtatttggat tacgttacaa
     2341 tacgtgtttt catcaaaatt tccctaccgg gcggtatcct gtttttgaag acggtaggat
     2401 ttgggtttgt ttgggggcaa tcccgcccaa attggagcaa tagatagggc tgtgtccgaa
     2461 tatgcggctc tgtgtgttga aacacagtta aaaatacgga agactttatg tccgaacaac
     2521 atatttcgac ttggaaaagt aaaatcaacg cattgggtcc ggggatcatg atggcttcgg
     2581 cggcggtcgg cggttcgcac ctgattgcct cgacgcaggc gggcgcgctt tacggctggc
     2641 agatcgcgct catcatcatc ctgaccaacc tcttcaaata cccgtttttc cgcttcagcg
     2701 cgcattacac gctggacacg ggcaagagcc tgattgaagg ttatgccgag aaaagccgtg
     2761 tttatttgtg ggttttcctg attttgtgca tcatttccgc cacgattaac gctggcgcgg
     2821 tcgccatcgt aaccgccgcc atcgtcaaaa tggcgattcc ctcgctgatg tttgatgccg
     2881 gcacggttgc cgccttgatt atggcatcct gcctgattat tttggtgagc ggacgttacc
     2941 gcgctttgga tcgcgtttcc aaaatcatca tcgttacttt gagtatcgcc acgcttgccg
     3001 ccgccggcat cgctatgtcg cgcggtatgc agatgcagcc cgattttatc gagccgacac
     3061 cgtggacgct tgccggtttg ggcttcctga tcgcgctgat gggctggatg cccgcgccga
     3121 ttgaaatttc cgccatcaat tctttgtggg taaccgaaaa acaacgcatc aatccttccg
     3181 aataccgcga cgggattttt gatttcaacg tcggttatat cgccagtgcg gttttggctt
     3241 tggttttcct tgcactgggc gcgtttgtgc aatacggcaa cggcgaagca gtgcagatgg
     3301 cgggcggcaa atatatcggg caattgatca atatgtacgc cgttaccatc ggcggctggt
     3361 cgcgcccgct ggtggcgttt atcgcgtttg cctgtatgta cggcacgacg attaccgtcg
     3421 tggacggcta tgcccgtgcc attgccgaac ccgtgcgcct gctgcgcgga aaagacaaaa
     3481 cgggcaacgc cgaattcttt gcctggaata tttgggtggc gggcagcggt ttggcggtga
     3541 ttttttggtt tgacggcgta atggcgaatc tgctcaaatt tgcgatgatt gccgcctttg
     3601 tgtccgcccc cgtgtttgcc tggctcaact accgcctggt caaaggagac aaacggcaca
     3661 agcttaccgc cggtatgaat tttcttgcca ttgtcggctt gctctacctg accggatttg
     3721 ccgttttgtt cctgttgaac cttagcggac ttttggcata ggtttcaaaa tctgaatgcc
     3781 gtctgtaata ttttcagacg gcattgccct ttcctagaaa aaaacaccga catcgcgtaa
     3841 aatatgcaca aattttgtga ttcaatcagg tcgtctgaaa cagattgcgc ctgatttatt
     3901 ttttcggaaa ataccatgag cgaacaaaac catccgcaaa tcgagccgca gttggacgaa
     3961 aaccaaatta tcgccctgcg ccgtgaaaaa ctgcacaaca tccgccaaca gcgcaacgcc
     4021 taccctaacg atttcaaacg cgacagcttt gccgccgatt tgcacgccca atacggcgaa
     4081 atcggcaaag aagaactcga cccgcaggcc gttcccgtca aagtggccgg ccgcatgatg
     4141 ctgaagcgtc aaatgggcaa agccagcttc gccaccattc aagacgttac cggtcaaatc
     4201 cagctttatc tgaacaacaa aggcgtgagt caggaagttt tggacgattt caaccattgg
     4261 gatttgggcg atatcgtcgg cgcggaaggt actttgttca aaaccaacca cggcgaattg
     4321 accgtgcgcg tgtccgacat ccgcctgttg tccaaatcct tgcgtccgct gccggacaaa
     4381 cacaaaggct tgagcgatca ggaaaccaaa taccgccaac gctatgtcga tttgattgcc
     4441 aacgaagaat cgcgcaatac ctttatcaaa cgcagccaaa tcatccaatc cgtgcgtaat
     4501 tttatggtgg gcgagcatta tctcgaagtc gaaaccccga tgatgcaccc gattcccggc
     4561 ggcgcgacgg caaaaccctt cgttactcat cacaatgcct tagatattcc gctttacctg
     4621 cgtatcgcgc ctgagctgta tttgaaacgc ctggttgtcg gcggcttgga acgcgtgttt
     4681 gaaatcaacc gcagcttccg caacgaaggc atgtccgtgc gccacaaccc cgaatttacg
     4741 atgattgaat tttacgaagc cttctccgac tacgaacgta tgatgcagat ggcagaagac
     4801 atcatcagaa acgcatcgtg cacagtaaac ggtacggcaa acatcactta caacggcaaa
     4861 gaagtcgatt tggaaagccc gtttgagcgt ctgaccatcc tcgaagccat caaaaaatac
     4921 aatccgcact acaccgacga gcagttgaac gatgcggaat ggctgaaaaa agaaatcgtc
     4981 aaacacggcg aaagcctgcc gccgtccccg ggcatcggca gcctgcaact cgcgctgttt
     5041 gaaggttgcg ccgagggcaa gctgtggaac ccgactttca tcatcgatta cccggtcgaa
     5101 gtttccccat tggcgcgcgc ttcggatacc aaacaaggtc tgaccgaacg tttcgaattg
     5161 ttcgttgtcg gccgcgaact ggcaaacggc tattccgagt tgaacgaccc cgaagaccaa
     5221 gccgaacgct tcaaagcgca agtggcgcaa aaagacgcgg gcgacgacga agcgatgcac
     5281 tacgatgccg actacatccg cgcgatggag ttcggtctgc cgccgacagg cggttgcggc
     5341 atcggtatcg accgcttggt aatgctgctg accgattcgc aaaccatccg cgatgtgatt
     5401 ctgtttccgc agatgcgccc cgagtaatca taaaaacagt tgaaatggca atgccgtctg
     5461 aacccgattg gattcagacg gcattttgta tggcaggatt gatagtgaat taacaaaaat
     5521 caggacaagg cggcgagccg cagacagtac aaa
//
LOCUS       DAXHSJ010000132         5551 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000132 DAXHSJ010000000
VERSION     DAXHSJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.132"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(43..810)
                     /locus_tag="ACFM8U_001665"
     CDS             complement(43..810)
                     /locus_tag="ACFM8U_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC6334870.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(898..1725)
                     /gene="mutM"
                     /locus_tag="ACFM8U_001666"
     CDS             complement(898..1725)
                     /gene="mutM"
                     /locus_tag="ACFM8U_001666"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC6334871.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1777..2442)
                     /locus_tag="ACFM8U_001667"
     CDS             complement(1777..2442)
                     /locus_tag="ACFM8U_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6334872.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVSSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPEWDGYLGGKALAAGAAR
                     VAD"
     gene            2621..4597
                     /locus_tag="ACFM8U_001668"
     CDS             2621..4597
                     /locus_tag="ACFM8U_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6334873.1"
                     /translation="MSKLKTIVLTASGLSVCSGSLYAQNTSSHQIGLAIMRLNSSILD
                     LHPTKQYFQSGSLWGELRQGFQMGEVNPELVRRHESKFIASRSYFDRVVNRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIAAPQSFGMNISDIDNKPYFQAIEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISASPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4665..5357
                     /locus_tag="ACFM8U_001669"
     CDS             4665..5357
                     /locus_tag="ACFM8U_001669"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC6334874.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGMLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAAGNRVLHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
BASE COUNT         1447 a         1791 c         1200 g         1113 t
ORIGIN      
        1 tccacaaaag cgaatcatac tgccacattc cgacaaccgg tttcagacag cgatatccgc
       61 atcgtcggcg acaacggcac ggatgctttc ttcgatttta tcttttaaag cataacggtc
      121 ttcgctttcc gccgcatccg ccacgcaaac gaaatcgact tttatcgtca attttttcat
      181 agacacgatg cgccacaggc aggtcggcaa accgacatcg gcatatgagg gacgagccgt
      241 cctttttccc gtttcgtcat aataacgcag cgcgaccgcc aaaacctttg cccccgcatc
      301 gatggcggat tggaacagcg cggctttgaa cggcaaaagc cccaatccgg aggaagtccg
      361 cgcttcgggg aaaaaactga cgttttgacc gcgttgcaag gtttcgcaga cggcgcggtt
      421 aatcggttcg atgtcgcgcc gcgaattgcg gttgatgaac accgttcccg cgttctgccc
      481 catcttgccc aataccggcc agcttttgat ttcctgcttg gcgataaagc tgctcggata
      541 aaccgcgctc atcgcgaaaa tatccagcca ggacacgtgg ttggcggcaa ccaagacacc
      601 gttcggatgt tcgggtgcgg gtctgcccac ctccaatccg atatccaaag ccgccaaaac
      661 ccccctgccc aactcgatta ccgcccgatt gcgcgactcg gggcagccgc cgtcaatacc
      721 gcgcaggttt ttgccggttt tgaatagcca gaccgtcaag cggcacaagc ggcgcagacg
      781 tgtaaaaaat gaagctttat ttgaagacat gaacaattct ttcccgataa tcggtgtttg
      841 ttgttgaaac gttcggacgg cataccgata aaatgccgtc tgaaaccgtt ttcagcccta
      901 tttctgacaa ttcgggcagt aaaacgtgcc gcgctgcccc aaagtttctt tcacaaccaa
      961 accgccgcac tgggggcacg gctgattgtg ccgcccgtac accgtatatt cctgttggaa
     1021 gtagccgctt ttgccgtcgc tgtccacaaa gtccctcagc gtactgccgc ccgtttcaat
     1081 ggcgcgccgc aacaccgctt tgacggtttc aaccaaaagc gcacactctt tctttttcag
     1141 gcggttggca ggacggtggg gcgaaatgcc cgccctaaac aggctctcgt tggcataaat
     1201 attgcccaca ccgaccacga ccgcattgtc catcagggca agtttgaccg cgcgcttctg
     1261 cgccttcagc cttgcataca gataatccgc acaaaatgcc tccgacaaag gctccggccc
     1321 cagtttttcc agcagcggat gatgctcttc aattccctca taccaaagta tcgcgccgaa
     1381 ctttcgcgga tcgcggtaac gcatgaccgt gccgtctgaa aacacaatat cgacgtgatc
     1441 gtgtctgtcc ggcctgccga tacgtccgtc cgacggcgta aaaatccgca agctgcccga
     1501 catccccaag tgaatcagca aaatccccgt ttggaaacgg acaatcagat acttcgcccg
     1561 cctgccgcag gacaaaacct gccgaccgga caaaatctcc cccaaatcgg gattaatctg
     1621 ccagcgcagc ttcaattggc gcaataccac ggcttccacc gttttccctt caatatgcgg
     1681 cgcgatgccg cgcaacgtcg tttccacttc cggcaattca ggcataaccc ctcccgacat
     1741 ttcttctgac agatgccgtc tgaaagacgg ctgtccctaa tccgcaaccc ttgccgcacc
     1801 cgccgcaagg gctttgccgc ccaaataccc gtcccactcg ggcaggggcg ttacgcccgc
     1861 cgcccttttg accaaaacca aaccgtacac tgcggcacat tgcggccacc aacggtcgcc
     1921 cgccttttcc ataaaccgcc aaaagcgtat ttgcccgagc gacgaaacgg gcggcagata
     1981 caccataaat ttcccaaatt caatatcgaa accggcatcg gcaagccgcc ttttcaactc
     2041 gggcaacggc agacaaaacc gtttttccgg caggcgttcg ccgtcaaacc aacggctgaa
     2101 tccccagagc gaatacggat tgaaacccgt cagcatcaag cgcccgcacg gtttcaatat
     2161 ccggtgcgct tccgacagga tttgcgaaga aacaccgcct tcaagcgtat gcggaaaaag
     2221 cagcatatcc gcagaaacat ccgccaaagc catattctcc gccgacatcg acatatctcg
     2281 cggcacacag acaacatctt cagacaggct cagccacgga ccgcccacct gaaccgcaca
     2341 cattcccgaa aaacggtatg aatccagata ccgcccgaag aaatcctgtt ccaattttgc
     2401 aacataccgc cccatcgccg tatcttcaaa ccatgcatcc atattgcgtc cgtttcaaac
     2461 agattgcctg ccgatattct attccaaaca ggatttctgt caaaaaacac atcggccgcc
     2521 catttccgaa tccgcataaa gttcctgtaa aacttgacgc tttttcagtc aaacagtacc
     2581 atcggacgat aaaatatgtt tattccgccg aatataaatc atgtccaaac tcaaaaccat
     2641 cgtcctgact gcctcgggtc tctccgtctg ttccggctca ctatacgctc aaaacacctc
     2701 ttcacaccaa atcggtttgg cgattatgcg cttaaactct tcaatactcg acctgcaccc
     2761 gacaaaacaa tatttccaat ccggcagcct gtggggcgag ctgcgccaag gcttccagat
     2821 gggcgaagtc aatcccgaac tggtacgccg ccacgaaagt aaattcatcg caagccgcag
     2881 ctatttcgac agggtcgtca accggagccg accctatatg taccatattg ccaatgaagt
     2941 caaaaaacgc aatatgcccg ccgaagccgc cctgcttccc ttcatcgaaa gcgcgttcgt
     3001 caccaaagcc aaatcgcatg tcggcgcatc gggcctgtgg cagtttatgc ccgctaccgg
     3061 caggcattac ggcttggaaa aaacaccggt ttacgacggc aggcacgacg tttacgccgc
     3121 caccgatgcc gcactcaact acctgcaata cctctatgga ctgttcggcg actggccgct
     3181 cgcctttgcc gcctacaact ggggtgaagg caacgtcgga cgcgccatca accgcgcccg
     3241 cgcccaaggg ctcgaaccga cctacgaaaa cctgcgtatg cccaacgaaa cgcgcaacta
     3301 tgttcccaag ctgctcgccg tgcgcaacat cattgccgcc ccccaatctt tcggcatgaa
     3361 tatcagcgac atagacaata aaccctattt tcaggcaatc gaaccggacc gtccgctcga
     3421 caacgaagcc atcgcccggc ttgccggcat cacgcaaagc gagctgctcg ccctaaaccc
     3481 cgcattcaac gtccccgcgt tcatccccaa aagcaaacgc aaactgctgc ttcctgtcgc
     3541 atccgtccaa accttccaaa gcaactacct caacgccgca cccgacagcc tgttttcatg
     3601 ggaagtctat acgcctgccg ccaaaaccag cctgtccgac atctcgacgg caaccggcat
     3661 gagcattgcc gacatcaaac gcctcaacaa cctgaacggc aaccttgtca acgcaggacg
     3721 cagcatcctt gtcgccaaga acggcaaaac ccttcagacg gcatcggaat ccgtcgtttc
     3781 catcgacatc gacaatacgc ccgacaccta ccgttccaat atgccggcag gcacggtgaa
     3841 cgtcggcatt gcccgaatcc gacccgccgc cgcacagaca gcggacatta ccgtcgcacc
     3901 tttgccgcag aaaaccgtcc gtacggaacc cgatcccctt gtccgtattg ccgaacctgc
     3961 ccttgcgaca gccgcagcgc aacctcaaac cgaaaaacag accgccatgc cgtctgaaac
     4021 ccaaaccgca acactcgcgc agatcatccc ccaaaacgac atgcaggcgg cagacgaact
     4081 catgcagctt gttgcccgaa acaacctgcg ccgacaggct gaagaaacca tctccgccgt
     4141 catcggcacg cctgacacag ttgccgaaca caacatttcc gcatctccgc aacataccgt
     4201 tgccgccgac ggcaaacgcc gggcacgttt ggaaacgcgc gtagccaaag ctgccgacgg
     4261 cgaagccgaa atctccccgc tccatgccag catccaccgc gttgtagaag gcgacaccct
     4321 gttcaacatt gccaaacgct acaacgtcag cgtagccgac ctgattgtcg ccaacaacat
     4381 caaaggcaac accatccaaa aaggacaggt actccgcctg gcgcaggcag cccctgccca
     4441 aacccgtatt gaaaaagtat cctacaccgc gcgcaaaggc gacaccttca aaagtatcgc
     4501 cgcgcgcttc aatatccata tcgacgacat ccgccggctc aatcccaacc tgaacaccat
     4561 caatccggga cagagggtca aactgattgg aagctgattc ggatacggca catgacagga
     4621 ctttctcagt cccgtctttt tcatcccaca tttcccatac catcatgaaa ctcatcaaat
     4681 acctgcaata tcaaggcata ggaagccgca agcagtgcca atggctgatt gctggcggtt
     4741 atgttttcat caacggaacc tgcatggacg acaccgatgc agacatcgat tcctcatccg
     4801 tcgaaacgtt ggatattgac ggggaagcag taaccgtcgt tcccgaaccc tatttctaca
     4861 tcatgctcaa caagcctgaa gattacgaaa cttcgcacaa acccaagcac taccgcagcg
     4921 tattcagcct gttccccgac aatatgcgga acatcgatat gcaggcggtc ggcaggctgg
     4981 atgcggatac gactggcgta ttgctgatta ccaacgacgg caaactgaac cacaacctga
     5041 cttcgccgag cagaaaaatt cccaagctat acgaagtaac gctcaaacac cccacaggag
     5101 aaacgctctg cgaaaccttg aaaaacggca tgctgctcca cgacgaaaac gaaaccgttt
     5161 gtgccgccga tgccgttttg gaaaacccga ccaccctgct gctgaccatt accgaaggaa
     5221 aataccacca agtcaaacgc atgatcgccg ccgccggcaa ccgcgtgcta caccttcatc
     5281 gtcggcgatt cgcacatttg gaaacagaaa acctcaaacc cggggaatgg aaatttatcg
     5341 aatgtccaaa attctgaaat aacatccaag aattccattt atattcatcc acatactcat
     5401 taaatatatg gtttaaccca atttaacgca aaaaatcaat ttacgatata atccatttgt
     5461 ctgagtaacg cctgttcaag caggcttatg agtaagacgt tttccccgta atgtgttttg
     5521 ccatctatac ttctcccctt gtatagatgg t
//
LOCUS       DAXHSJ010000133         5541 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000133 DAXHSJ010000000
VERSION     DAXHSJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.133"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(77..277)
                     /locus_tag="ACFM8U_001670"
     CDS             complement(77..277)
                     /locus_tag="ACFM8U_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334875.1"
                     /translation="MIGRLFRIFFFFALAALIIKRLFSRRQKRALCEVAKISAWVLLG
                     SAAATLFWYLVMLYFKHIPDSY"
     gene            437..1576
                     /locus_tag="ACFM8U_001671"
     CDS             437..1576
                     /locus_tag="ACFM8U_001671"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC6334876.1"
                     /translation="MSQNASVGIVTPQKIPFEVPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGKHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIEQWAAVIGGSLGGMQA
                     LQWTISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKGCQQ"
     gene            1573..2154
                     /gene="metW"
                     /locus_tag="ACFM8U_001672"
     CDS             1573..2154
                     /gene="metW"
                     /locus_tag="ACFM8U_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC6334877.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            complement(2176..2282)
                     /locus_tag="ACFM8U_001673"
                     /pseudo
     CDS             complement(2176..2282)
                     /locus_tag="ACFM8U_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2376..3212
                     /locus_tag="ACFM8U_001674"
     CDS             2376..3212
                     /locus_tag="ACFM8U_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
                     /protein_id="HFC6334878.1"
                     /translation="MKNTTSSLLLWLAAIMLAACSPSKNDKTKEVGASAASSSASSAS
                     SQTDLQPAASAPDNVKQAESAPPSNCTGLHPASGIDDLMQQIAEHIDSDCLFALSHHE
                     LETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDALME
                     QERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYIGQL
                     PGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNPNLG
                     RR"
     gene            complement(3262..3822)
                     /gene="efp"
                     /locus_tag="ACFM8U_001675"
     CDS             complement(3262..3822)
                     /gene="efp"
                     /locus_tag="ACFM8U_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC6334879.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(3865..5013)
                     /gene="earP"
                     /locus_tag="ACFM8U_001676"
     CDS             complement(3865..5013)
                     /gene="earP"
                     /locus_tag="ACFM8U_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC6334880.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWYAEAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSGALRKRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYSPETASVHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFEQPSAPEKLAAFVSKHQKIR"
     gene            complement(5032..5310)
                     /locus_tag="ACFM8U_001677"
     CDS             complement(5032..5310)
                     /locus_tag="ACFM8U_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334881.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
     gene            complement(5321..>5541)
                     /locus_tag="ACFM8U_001678"
     CDS             complement(5321..>5541)
                     /locus_tag="ACFM8U_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334882.1"
                     /translation="QTDAPGFQYARACCRLVLRHRRQRPIFRGTPVSGVFKGSAVRCF
                     PAARLQTASPVRPLDKACAWGDNGVLLF"
BASE COUNT         1382 a         1422 c         1456 g         1281 t
ORIGIN      
        1 acctcctacc gcaagccctt ttcatcaaat atgccgtctg aaacagaaaa atgcgtttca
       61 ggcggcattt tttccgtcaa tacgaatccg gaatgtgttt gaaatacagc atgaccaaat
      121 accaaaacag tgtcgcggcg gctgagccaa gcagtaccca tgcgctgatt ttggcgactt
      181 cgcacagggc gcgtttttgc ctgcggctga aaaggcgctt gataatcaac gcggcaagtg
      241 cgaaaaagaa aaaaatacgg aaaagtctgc ctatcatgtc gggaagctgg gttaggaccg
      301 tctgaaaaag tatggttcgg ttgcggcggc atgggaacga atgtttcggc gtgtcaggct
      361 gcttgtccga ccgtgtcaaa aaatcatgcc gtttgcgata taataacgct tttcgccgca
      421 tttgttggaa caggatatga gtcaaaatgc ctcggtgggc attgtaacgc cccaaaaaat
      481 tccgtttgag gtgccactgg ttttggaaaa cggtaaaact ttgccgcgtt tcgatctgat
      541 gattgaaacc tacggcgagc tgaatgctga aaaaaacaat gcggttttaa tctgccacgc
      601 gctgtcgggc aaccatcatg ttgcgggcaa acattcggcg gaggataaat atacgggctg
      661 gtgggacaat atggtcggtc ccggaaaacc gattgatacg gaacgttttt tcgtggtcgg
      721 tttgaataat ctgggcggct gcgacggcag cagcgggcct ttgtcgatta atcctgaaac
      781 gggcagggaa tacggcgcgg attttccggt ggttacggtg aaggactggg taaaatcaca
      841 agccgcgctt gccgattatc tcggcatcga acaatgggcg gcggttatcg gcggcagctt
      901 gggcggcatg caggctttgc agtggacgat ttcctatccc gaacgcgtgc gccacgcctt
      961 ggtgattgcg tccgcgccga agctttccac gcaaaatatc gcgttcaacg atgtggcgcg
     1021 tcaggcgatt ttgaccgatc ccgatttcaa cgaaggacat taccgcagcc gcaacaccgt
     1081 tcccgctcgg ggcttgcgga ttgcccgcat gatggggcac attacctatc ttgccgaaga
     1141 cggtttgggc aaaaaattcg gacgcgattt gcgttccaac ggctatcaat acggctatgg
     1201 cgttgaattt gaagtagaat cctatctgcg ctatcaaggc gacaaattcg tcgggcggtt
     1261 tgatgccaac acctatttgc tgatgaccaa ggctttggac tatttcgatc cggcggcgga
     1321 tttcggcaac agcctgaccc gcgccgtgca ggatgttcag gcaaaattct ttgtcgccag
     1381 cttcagcacc gactggcgtt tcgcgcccga acgttcgcac gaactggtca aggcactgat
     1441 tgccgcccaa aaatccgtgc agtatatcga agtcaaatcc gcacacgggc acgatgcctt
     1501 tttaatggaa gacgaagcct atatgcgtgc ggtcgccgcc tatatgaaca acgtttataa
     1561 gggatgtcag caatgaacct gcgcgatgat ttgcaactga tttacgaccg gatacccgaa
     1621 ggcagccgcg tcttggactt gggctgcggc gacggcgaac tgcttgccgc attggtcgaa
     1681 cacaaaaaat gcagcggcta cggcatcgaa atcgacaccg acagcgtgat tgccgcgatg
     1741 tcgcgcggcg tgaatgttat ccaagccgat ttggaagaag gtttaaccgc attcaacgat
     1801 caaagttttg atgtgattgt gttgagccaa accatccaag cgatgcagaa taccgaaaaa
     1861 atcctgcgct gcctgatgcg cgttgccaaa caggcgattg tcagcttccc gaatttcggc
     1921 tactggcgca accgcgtcca aatcgcactc ggcggacata tgccggtttc cgaacgtatg
     1981 ccctaccatt ggtatgatac gccgaacatc cattggtgta cgctcaaaga ctttgatttg
     2041 ttgtgcgcca aaaacaacat acgcgtcctc gagcgcgccg tgatgacggg caacagacag
     2101 gtcaaacatt tccccaacct tttgggcagc cttgcgtttt accgcgtcgg gtaagacagg
     2161 ttgagaacct tgttggtata gtggattaaa tttaaaccag tacggcgttg cctcgccttg
     2221 ccgtactatt tgtactgtct gcggctcgcc gccttgtcct agtttaaatt taatccacta
     2281 taataacgag gttcttccgt tgtactaaca agaagccgtc tgaaaaatga aacggtaggt
     2341 tttcagacgg cctctttaat attggaaaca atagaatgaa aaacacaacg tcatcacttc
     2401 tcttatggct tgccgcaatc atgctggccg cgtgttctcc gagcaaaaac gataaaacca
     2461 aagaagtcgg tgcatccgct gcttcgtcct ccgcgtcatc agcttcttcc caaaccgatt
     2521 tgcaaccggc cgcatccgcc cctgataacg tcaagcaggc agaaagcgcg ccgccgtcaa
     2581 attgcaccgg cctgcacccc gcctccggca ttgacgatct catgcagcaa atcgccgaac
     2641 acatcgactc ggactgtctg tttgcccttt cccatcacga actggaaacc cgtttcggct
     2701 tacccggcgg aggctatgac aacatacagc ggctgctgtt tcccgacatc cgccctgaag
     2761 atcccgacta ccatcagaaa atcatactgg caattgaaga cttgcgttac ggaaagcgca
     2821 cgatcagccg gcaggcacaa gatgccttga tggaacagga acgccgcctc cgagaagcga
     2881 cgctgttgct gatacagggc agtcaagaaa cccgcggaca aggcgaggag ccgaaacgca
     2941 cgcgttattt tgaagtttcg gcaacccctg cctattcgag ccggcacaac aacggacttg
     3001 gcggcaattt ccaatacatc ggccaattgc ccggctatct gaaaatacac ggagaaatgc
     3061 ttgaaaacca atcactcttc cggctgtcca accgtgaacg caatcccgac aaaccgtttt
     3121 tagacatcca ttttgacgaa aatggcaaaa tcacgcgtat tgtcgtttac gaaaaaaaca
     3181 tctacttcaa tccaaacttg gggcgaagat aaaaaaatgc cgtctgaaac gttttcagac
     3241 ggcatttttc aatgcaacaa atcaagcgcg tttgcggaat tcgccggtgc gggtatcgat
     3301 ttcgattttg tcgccgtttt cgatgtaaga catcacttgg atttcggtgc ccccgaccag
     3361 gcgcgcggtt ttcatcactt tgcctgaagt atcgcctttg acggcaggct cggtatactc
     3421 gacttcgcgt acgatgatgg tgggcagttc tacggagatg gggttgcctt cgtagaaggt
     3481 aacttcgcat tggtcttcca taccgtcaac gatgaatttc aacgcgtcgc cgatgttgtc
     3541 ggcttcgatt tcgtattggt tgaattcttc atccataaag acgtacatcg gatcggcaaa
     3601 gtagctgtac gtacagtttt tgcgggacag gatgaccacg tcgaatttgt cgtcggcttt
     3661 gtaaatggtt tcggaagccg cgccggtcag caggtttttc agtttcatgc tgactttggc
     3721 ggaagagcgg ccgcctttga tgtattcggt tttttgaacg accataggat cgttgccgac
     3781 cataaataca ttgccggcgc gcagttcttg tgctgttttc attgtgattt tccaatcaaa
     3841 tcggttgtcg taaaacgcgc tattctagcg tattttttga tgctttgaaa caaaggcagc
     3901 gagtttttca ggagctgacg gctgctcgaa aagataacgg ctccaatcct ccgcgccttg
     3961 ccgccagccg ttttgatgtt gttgcagggt ttgccaacat tcgaggcgtt gcgtggcgga
     4021 taaagcctct ccgccgttga ggtcgtctga aaggcggcgg tgtaccgatg cggtttcggg
     4081 cgagtaaaat ccatgcgcct tatcccaaaa ggcgtggagt ttatcgagat ggacattctc
     4141 gtcttgcggg tagatgtgcc aaaagaaggg tttgccggca agctgggcgc gcacgaaact
     4201 gtcttcgccg cgtatcacgg cgcagtcggc gaggtgcagc agtttgtcga agtcctgttg
     4261 cggcacgaaa gggattttga cgaggcggac ggatgccgtc tgaaaaacat cgccgtcgtt
     4321 ttgcagggca tcttgcggaa taacgccgct ttgtttgagg ctgtcgatga tttgcgtccc
     4381 cgccagcaac agtgtcatcg ggctgcctgc ctgtcgccac atttccagcc actttgccca
     4441 aacatcgctc cgatagccga aaagcagcca ttcgggggcg tttttttcgg gaagcatcag
     4501 cctcttgcgc aaggctccgc tatcgaaacg gacggcttcg cagtaatcgc gttcgcgtat
     4561 cagtccgccg cttttttcgc tgaagcccat aaaccaaaaa tatttttgaa caccttcctg
     4621 cggcgaaggc atcaggtgca gcctttcatt gctttcctcc gcgctcaaat attcccaatt
     4681 cagccaaagc ggcttgtgtc ggcggataat gtgcagcaca ttttcgggca ggtcgcaggc
     4741 aaaagtttcg atgacgacat cgggaacagg cgcggtatca atgtctgccg cttcggcata
     4801 ccaagtgcgg acatgaatat cctgatgaac gcagggaaca tcgggcaaat caggacaaag
     4861 cgcacgcaag gcggacacat cgtccgtcca caaatgcacc tgccaaccga gttcgcggtg
     4921 caaaacacgg gcaagccgcc acgaaacgcc gatgtcgccg aaattgtcga tgaccttgca
     4981 aaaaatccaa cagacaaaag gaggagtatt catagaaata acggaaacgg gctaaaacgc
     5041 gccaaaatag tggaacaaat acacaatcag caaaataatc ggaataaaca gcaacatagt
     5101 ttgacgtttc aaatcaaact cttgtttcaa atgatgcttg atggtgtcga aaaggacgaa
     5161 tttgaacaaa aacgtcgctt cgcaggcaag gatgatgccg ttaatcagat gctggggttt
     5221 gtcttcgggc ggattggcgg cgaaaacggc aagcgtcgcc aagggtatca ggcagacgac
     5281 aagccagcgg acaagcaggt ttcgggacat tgcaaggctt tcaaaaaagc aaaacgccat
     5341 tatcgcccca agcgcacgcc ttgtctaacg gacgcaccgg agatgccgtc tgaagccttg
     5401 ccgcaggaaa acaccgcacc gccgaacctt taaatacgcc cgagacgggc gttccccgaa
     5461 aaatcggacg ctgccgccga tgcctgagga caaggcggca gcaggcacgg gcatattgaa
     5521 aacccggcgc gtcggtttga a
//
LOCUS       DAXHSJ010000134         5537 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000134 DAXHSJ010000000
VERSION     DAXHSJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.134"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(8..970)
                     /gene="mafA"
                     /locus_tag="ACFM8U_001679"
     CDS             complement(8..970)
                     /gene="mafA"
                     /locus_tag="ACFM8U_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6334883.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAVYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVEADNNHEGYGYSDEVVRQHRQGQP"
     gene            complement(1154..1873)
                     /gene="pyrH"
                     /locus_tag="ACFM8U_001680"
     CDS             complement(1154..1873)
                     /gene="pyrH"
                     /locus_tag="ACFM8U_001680"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC6334884.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVITGEDEGTLVHC"
     gene            complement(2090..2944)
                     /gene="tsf"
                     /locus_tag="ACFM8U_001681"
     CDS             complement(2090..2944)
                     /gene="tsf"
                     /locus_tag="ACFM8U_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC6334885.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVISFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(3074..3802)
                     /gene="rpsB"
                     /locus_tag="ACFM8U_001682"
     CDS             complement(3074..3802)
                     /gene="rpsB"
                     /locus_tag="ACFM8U_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC6334886.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(3979..4212)
                     /locus_tag="ACFM8U_001683"
     CDS             complement(3979..4212)
                     /locus_tag="ACFM8U_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334887.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            4290..4871
                     /locus_tag="ACFM8U_001684"
     CDS             4290..4871
                     /locus_tag="ACFM8U_001684"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC6334888.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVWAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRVAQKRGAKLYLPYIEPRSQRMWFTPYPESGMERERIRGR
                     AKLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVGTLPREAHDLPLDGFVSEAGILCF"
     gene            4932..5522
                     /locus_tag="ACFM8U_001685"
     CDS             4932..5522
                     /locus_tag="ACFM8U_001685"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6334889.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDLGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMYMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         1233 a         1270 c         1542 g         1492 t
ORIGIN      
        1 gtgtgaatca aggttgtcct tgtctatgtt gtcgcactac ttcatcgctg tatccatacc
       61 cctcatgatt gttatcagcc tctacggatg gggcggagtt acccgtatga ttgccgtatg
      121 gccggatatc ggagaaatcg accattaatc cttctgtcgg tttaattcct ttgcttactt
      181 tatacggccc catccacaat gcgtaatttt ctttatagac agcttcaaac gcattggttt
      241 ttggtttgat gagcaatttt ttattggttc tgtctactgc gaaatattcc agttttgttt
      301 gggctttcag tgtttcggca ttgtataggt gcatttcggt tctgttgcgt atcgttccaa
      361 atacgtcgat gttaataaac acatctgtat cggcattggc aggagaaaca acgtctatgc
      421 cgcgcaggaa aaatacggtc tgcaccaagt gggaaagaaa ggcagtgtcg cgcgggttag
      481 tcgtcaaggt ttcatttcga taatccccca tcccgccaat atttaagccc agactgctcc
      541 tacttccgct accgtctgat tgggtgcgcg agagtgcagg ggcattaagt gtagataaag
      601 aagtggttaa acctgtcaaa ccgcctgatg ttgtttcagc ggtggtttcg taacgtggat
      661 aggtgtaatc ggtacggacg gcagggctgt ttatgtattc gccacgaatc agtgcatcaa
      721 tggagtagcg acctcctgtc aaactgcctg aaccttggtc gcccatagtt gcaatgtaca
      781 atgcaacttt tcgtccgtgt aatgcctgta aatccatgtc tttaacggca gctctggcag
      841 aagcggccac aagttcttgt tcgaccgcaa agcgtttacc tccgccatgc gatggaatac
      901 ctgtcagtgt cccgcaggcg gataaaataa aaactgaaaa aagaataggt atcagcagcc
      961 gtgcttgcat agattttctc ctttgatgaa aaacaaattg tatcaaaatt gtaaatatag
     1021 tggattaaat ttaaaccagt acggcaaggc gaggcaacgc cgtactggtt taaatttaat
     1081 ccactataat ctccatataa taaatggctg tctgaagccc atatgcgact atatctgccg
     1141 acactatggt caatcagcag tgaaccagcg ttccctcgtc ttcgccggta atgacgcgtt
     1201 tgagcgagcc ttctttggcg atgccgaaga cgacgatatt gagcttgcgt tcgcggcaga
     1261 gggcgaaagc ggtcgcgtcc atgactttga ggtttttcaa caaggcttcg tcaaaagtaa
     1321 tggtttcgta gcgtgtggcg gacgggtctt ttttcgggtc tgcggtgtat acaccgtcga
     1381 cgttggtggc tttgagcatc acgtcgcagt tcatttccgc accgcgcaat gcggcggcag
     1441 tgtcggtcgt gaagaacggg ttaccggtac cggcggcaaa aatcacgact ttgccttctt
     1501 ccaaatattg gatggctttg gggcgggcgt aggtttcggc gatttgctgc atagacagtg
     1561 cggattgtac gcgcgctttg atgcctaaag tttcaaatgc gtctttgagt gccaacgcgt
     1621 tcatcacggt cgccatcatg cccatgtagt cggcggtggc gcgatccatg ctgcctgctt
     1681 gggcggatac gccccggaaa atattgccgc cgccgacaac aataccgact tgcacgccca
     1741 ttttaacgac ttcggcaatt tcgccgacag tttgaacgat ggtatcgtga ttgatgccga
     1801 acggatcgga acccatcagg gattcgccgg agagtttcag taatacgcgt ttgtatttga
     1861 tttgctgtgt catggatacc ttgctttctt gaatgtcgtc ggtcggagtt gggtcgtctg
     1921 aatttcaaac ggtcggaata ttaatgatag ggtgtgtcgg tttattattg ttttgtttta
     1981 agacggtctg aatattcggt cgtcttaaaa taggtaccgt aaacggtttt ctcaaaaaaa
     2041 agcaccctga ttcgtttgga atccaggtgc tttctttttc ataagtgcct tacactttgg
     2101 cggctgcggc tacttcggct gcgtagtcga caacggcttt ttcgatgcca tcgcctactt
     2161 tgtagcgtac gaagctgatc acttcagtgc cgttttcttt agcgaattgg gcaacagttt
     2221 gatcggggtt catcacgaat gcttggccgt tcagagtgat ttcagccaag aatttacgga
     2281 tgcggccttc aaccatttta gcggcgatgt cggcaggttt gccggaagcg atggcttgct
     2341 cggtgtagat gtggcgttct ttttcaacgg tttcggcatc tacttcggct tcgcttacgc
     2401 attgtggttt agcggcaaca atatgcatac cgattttgcg tgctacgtct tcagagcctt
     2461 tgtactcaac caatacgcct tcggtcgcca atgcgccgtg gatgtaggca accagttggt
     2521 tggcagtgtc gatcacttgg aagcgacgga cagacatatt ctcgcccaat ttggcgatga
     2581 tggctttgcg ttctgcttca accagttcgc tcagttcttc aacagaagcc ggttttttct
     2641 cggcagcagt tttcgcaacg aagttggcaa attctacgaa gcccgcgtct ttagcaacga
     2701 agtcggtttc gcagtttact tcgaccaatg cgccgacatt gccgttgatc gcgtaagcca
     2761 atacgccttc ggcagcggta cggccggcca gtttaccggc tttcgcaccg gatttgatac
     2821 gcaggatttc ttcggctttg tcgaagttgc cttcggcttc aaccaaggct tttttgcatt
     2881 ccatcatgcc caagccggta gcggcgcgca ggtcggcaac catttttgca gtaatttctg
     2941 ccattttgaa tctcctagat ttttgggaaa tacggaaagg attttccgca ttcgggaatc
     3001 gtgtgccgcg aacggacgtt cagacggcat atttgagaaa aggggcataa cgcccctctt
     3061 catgtatact gaattactcg gcagcagctt cttgggcagc ggctacggtt tcttgcagcg
     3121 cttggttttt gccttccaaa actgcgtcag cgatgccgcg gcagtacagg cggatggctt
     3181 tggcggagtc gtcgttgccg gggatgacgt atttcacgcc gtcagggctg ttgttggtat
     3241 cgactacggc gataacgggg atgcccagtt tttcagcctc gaccagagta cctttttggt
     3301 agccggtatc gataacgaaa atcgcgtcgg gcaggccttt catgtttttg ataccgccca
     3361 aagaacgttc cagtttttca acatcgcgtt gcatttccag aatttctttt ttgctgaaac
     3421 cgctttcggc agcattttcc aaggctgcgg ttttttcttc caggcgtttg atggattgct
     3481 taacggtttt gtagttggtc agcataccgc ccaaccagcg gtaatcaacg aaaggcatac
     3541 cggcacgggt cgcttcttcg cggatgatgt cgcgggcttg gcgtttggta cctacgaaca
     3601 atactgtacc tttgttggca accaaacggc gtacggcttc ttgcgcgtct tggaacatcg
     3661 gcagggtttt ttccaagttt acgatatgga ttttgttgcg tgcgccgaaa atgtattgtg
     3721 ccattttcgg gttccagaaa cgggtttggt ggccgaagtg gacaccggct tcaatcatct
     3781 gacgcatagt aatttgagac attttgattt ccttgaaagg gttaagggca cacattcaat
     3841 cgcatagaac ttgataggcg atgcctattg tcaagcaccc ttcggcgaaa gtgggcataa
     3901 gttttaaaaa ataaaagagt gtttccgaaa tggaaaactg acggattata gcggataaga
     3961 ggccgtctga aaagggattc agggttttgt gccgctgtct ttttgccgtt gtgcacgctg
     4021 ctgttcgaat tctgcctgtt cctgctgctt catcaagcgg ttgcggcggc ggacggttac
     4081 gacgaagacc gtgaacacgg tgggcagaac cgcccaaaaa accaaataaa tcaaggcgcg
     4141 cgcaatactc ggctgcgcgg cggaaaacat cacggcaaca aaaagatagc cgatggcgac
     4201 gatgtgccac attgtgtgcg tcctgtttga atgttaacaa aatcgttata atcgcaacat
     4261 tctaccgcaa tcctgaaagg ccgtctgaaa tgaggaacga ggaaaaacgc gccctgcgcc
     4321 gcgaattgcg cgggcggcgt tcgcaaatgg ggcgggacgt gtgggcggcg gcaacggtaa
     4381 aaatcaacca cctgctcaaa cgttatatta aaaaagggcg gaaaatcggc gtgtattggc
     4441 cgatgggcaa ggaattgcgt ttggacggct ttgtccgtgt cgcacaaaaa cgcggcgcaa
     4501 aactttatct gccttatatc gaaccgcgtt cgcagcggat gtggtttacg ccgtatcctg
     4561 aaagcggaat ggaacgggag cgcatacggg gcagggcgaa gttgaacgtg ccgcagtttg
     4621 cagggcgcaa aatccgcgtg cacggtttgt cggtattgct cgtcccgctt gtcggcatag
     4681 accgcgaagg ctaccgcttg gggcaggcag gcggctatta cgatgcgacg cttgcagcga
     4741 tgaaataccg tttgcaggca aaaaccgtgg gcgtgggctt tgcctgccaa ctggtcggca
     4801 cgctgccgcg cgaggcgcac gacctgccgc tggacggttt tgtgtcggag gcggggatat
     4861 tgtgttttta gccgttcaga cggcattccg ggcggaaatg ccgtctgaag gcggaatatt
     4921 gggagaaaag gatgtcggaa aagataatac tgccggtctt gtcattgggc ggcgttcggc
     4981 tcgaaccgtt ggatgtgcat catgaaacgg gtttgcgcga ggcggtttgc gatggggagg
     5041 tttggaagct gggggtaacg tccgcgcccc atccggatag ggtggcagac tatatcggga
     5101 cagcattggc aacgcgtttg gcgtttgcgg ttgtcgatga agaggcgggc agggtggtcg
     5161 ggacaacggc gtattatcat tttgaaccgc agattccgcg tttggatctc ggattcacat
     5221 ggtatgcggc atcggcacgg cggacacgga taaacacctg ttgcaaaatc atgctgttgg
     5281 attacgcatt tgatgttttg gcttgccgtt gcgtgggatg gcgcacggat attctgaacc
     5341 ttgcctcgca acgtgccatc gaacggctgg gtgcggaaaa agacggcgtg ctgcgtatgt
     5401 atatgctccg taaagacggc agtgtgcgcg atacggtcgt gtacagtatg ctgagggaag
     5461 attggtgcaa aaacagggaa atcctgaccg gcaggttggc agggtatggc gtgcaggtgt
     5521 gaggcagtat tttcaaa
//
LOCUS       DAXHSJ010000135         5470 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000135 DAXHSJ010000000
VERSION     DAXHSJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.135"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(250..1509)
                     /gene="rho"
                     /locus_tag="ACFM8U_001686"
     CDS             complement(250..1509)
                     /gene="rho"
                     /locus_tag="ACFM8U_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC6334890.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(1729..4113)
                     /gene="ppsA"
                     /locus_tag="ACFM8U_001687"
     CDS             complement(1729..4113)
                     /gene="ppsA"
                     /locus_tag="ACFM8U_001687"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC6334891.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYSGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4524..5345
                     /locus_tag="ACFM8U_001688"
     CDS             4524..5345
                     /locus_tag="ACFM8U_001688"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC6334892.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1166 a         1281 c         1597 g         1426 t
ORIGIN      
        1 ttatacaaaa atgagcgcgg ttcggcgcaa ccttgaatca agttcccgca tcggttttca
       61 ttgccggtac ggatgcgttc aagccggctt tgcaaaggcc gcgctttcgg caagcggaca
      121 cggacactgc cgacggttgc gccgttaacg gggggaggga ggagctgcgc cgaccgtgtg
      181 aatgaaagtg ccgtctgaaa cccgattttc aggcttcaga cggcatttcg cctaatgtcg
      241 atgatgtgtt tattttccgc gcatcagttc aaagaaatca tcattgtttt tagaggcttt
      301 gattttcccg tttaagaact cggtagcttc gatttcatcc atcgggtgca ggaacttgcg
      361 taagagccac atacgttgca actggtcgtt cggcacaagc agctcttcgc ggcgcgtgcc
      421 ggatttgttg atgttgatgg cggggaagag gcgtttttcc gccatacggc gatcaaggtg
      481 caattccata ttgccggtgc ctttgaattc ttcgtaaatc acatcgtcca tacggctgcc
      541 ggtttcaacc aatgcggtgg cgataatggt cagcgaaccg ccttcttcca cgttgcgcgc
      601 cgcaccgaag aaacgtttgg ggcgatgcag cgcgttggca tcgacaccgc cggtcaggat
      661 tttgcccgag gtggggacga cggtattgta ggcgcgggca aggcgggtaa tcgaatccag
      721 caggatgacc acgtcttttt tgtgttccac catacgcttg gctttttcaa gcaccatttc
      781 ggcaacttgg acgtggcgtt gagccggctc gtcaaaggtg gaggagacta cttcgccacg
      841 gacggagcgg ctcatttcgg ttacttcttc gggacgctcg tcaatcaaga ggacgatgag
      901 ttcgacttcg ggatagtttg cggtaacggc gtgggcaata ttttgcagca tcacggtttt
      961 accgcttttg ggcggggcaa ccaagagggc gcgctgacct ttgccgatgg gggaaatcag
     1021 gtcgatggcg cgtccggtca ggttttcttc ggactttaag tcgcgttcca gcttcaactg
     1081 ttcggtcgga aacagcgggg tcaggttttc aaacaggatt ttatggcggc atacttccgg
     1141 gtggtcgccg ttgatggtat caagcctgac cagggcaaaa tagcgttcgt tgtcttttgg
     1201 gacgcgcacg cttccttcga tggtgtcgcc cgtatgcagg ttgaagcggc ggatttgggt
     1261 gggcgagaca tagatgtcgt cggggccggc aagataggac gtgtccgcgc tgcggaggaa
     1321 gccgaagccg tcgggcagga tttcaagcgt gccggagcag gtgaaaccct cgcctttttt
     1381 catcatctgg cggacgatgg caaatacgag gtcttgtttg cggaatcggt tggcgttttc
     1441 gatgccgtgt tcttccgcca attctaagag tttggaaatg tgcagggttt gtaattcgga
     1501 gacgtgcata ataatgatgt attttgaaga ggaaaaagac aggcagatgc cgtctgaaag
     1561 aagaagctga ccgttgccgg ttgctcgggg aagggggaat tgtaggcagt cggcgcgtgg
     1621 gtgtcaaata ttatcgcgga cggggcatcg gcaggaaatg ccgtctgagc ggagctgctt
     1681 ggaaaaaaat acccccgcgc ttttcaggct cgggggtatg ggcattgatt atttgttcaa
     1741 ttcattcgcc aaatatagcc aagtttcgat gacggtatcc gggttcaggg aaacgctttc
     1801 aatgccttcc tcaaccagcc atttggcgaa gtccggatgg tcggacgggc cttgaccgca
     1861 gatgccgaca tatttgttct gcttgcggca ggcggagatg gcaagatgca gcatcacttt
     1921 gacggcaggg ttgcgttcgt caaacgattc ggataccaag ccgctgtcgc ggtcaagacc
     1981 gagggtcagt tgggtcatgt cgttcgagcc gatggagaag ccgtcgaagt attgcaggaa
     2041 ttgttccgcc aataccgcgt tgctcggcag ctcgcacatc ataatcaggc gcaggccgtt
     2101 tttgccgcgt tccaagccgt tttctttcag ggctttgaca acggcttcgg cttcgcccaa
     2161 agtgcggacg aacggaatca tgatttcaac gttggtcaga cccatttcgt cacgaacgcg
     2221 tttcaaggct ttgcattcca aggcgaaaca gtctttgaag ttgtcggcga cataacgcgc
     2281 cgcgccccgg aagcccaaca tcgggttttc ttcatgcggt tcgtatacgt tgccgccgac
     2341 taggttggcg tattcgttgg atttgaagtc ggacatacgg acgatggttt tacgcggata
     2401 aaccgatgcg gccaatgtcg ccacgccttc ggcgatttta tcgacgtaga agtcaacagg
     2461 ggacgcgtaa ccggcgatac ggcgggtaat ttccgctttt aattcgtcgt cttgtttgtc
     2521 aaattccaac aaggctttgg ggtggatacc gatttggcgg ttgatgataa attccatacg
     2581 cgccaagccg atgccttcgc tgggcaggtt ggcgaagctg aatgcgagtt cgggattgcc
     2641 gacgttcatc atgactttta caggtgcttt gggcatgttg tccaaagcaa catcggtaat
     2701 ttgtacgtcc agcaggccgg agtagatgaa gccggtatcg ccttcggcac aggatacggt
     2761 aacttcttga ccgtttttca gcaattcggt tgcattgccg cagccgacaa cggcaggaat
     2821 acccagttcg cgcgcgatga tggcggcgtg gcaggtacgt ccgcccctgt tggtcacgat
     2881 ggcggaagcg cgtttcatca ccggttccca atccgggtcg gtcatgtcgg taacgagtac
     2941 gtcgccggct tcgacggaat ccatctcgga agcatcttta atcaggcgta ctttgccctg
     3001 tccgactttc tgaccgatgg cgcggccttc gcataatacg gttttgtcgc cgttgatggc
     3061 gaagcggcgc aggttgcggc tgccttcttc ctgggatttg acggtttcgg gacgggcttg
     3121 caggatgtag agtttgccgt ccaagccgtc gcgtccccat tcgatatcca tcgggcggcc
     3181 gtagtgtttt tcgatggtca gtgcgtaatg cgccaactcg gtgatttctt cgtcggtaat
     3241 ggagaagcgg ttgcggtctt cttcggggac atcgacgttg gttaccgatt tgccggcttc
     3301 ggctttgtcg gtaaaaatca ttttgatgtg tttcgaaccc atggttttac gcaggatggc
     3361 gggcttgccc gctttgagcg tgggtttgaa cacataaaat tcgtccgggt tgaccgcgcc
     3421 ttgtacgacg ttttcgccca gaccgtaaga ggaggtaaca aagacgactt gatcgtagcc
     3481 ggattcggtg tcgagggtga acatcacacc tgatgcgccg ctgtcggaac gcaccatgcg
     3541 ttgtacgccg gcggaaaggg cgacgatgtc gtgttcgaag cctttgtgga cacggtaaga
     3601 aatggcacgg tcgttataca gggaagcgaa tacatggtgc atcgcttctt taacgttatc
     3661 caagccgttg atgttcaaga aggtttcctg ttgtccagcg aatgatgcgt ctggcaggtc
     3721 ttcggcagtt gcggaagaac gtacggcaac ggaaatgtcc gcaccgccgg catcggcaac
     3781 cattttgttc catgccgctt cgatttcggc atcgagctgt tcagggaaag gcgtgtccaa
     3841 aatccattgg cggatttctt tgccgacgcg tgccagttcg gcaacgtctt cgacatccaa
     3901 ttttgccagt gcggcggaaa tgcgttcgct cagaccgttg tgtgcgagga atgcgcggta
     3961 ggcatcggct gtggtggcaa agccgccggg gacgcgaacg cctttttcgg tcagctgact
     4021 gatcatttcg cccagcgagg cgtttttacc gcccacgcgt tcaacatctg tcatacgcag
     4081 gttttcaaac cagattacgt agttgtcggc catttgtgtg tccaatccaa aatatgttaa
     4141 aaaagaaaca aatccgcgtg cttattttaa gcgattcgtt cctctgctgt catgtgtttt
     4201 atccgtttta aaatcatgat gccgtctgaa aaattgcggt ttcggcgtgt gtagcggttt
     4261 gaaacttgca gccggtatac ttcttttttt gggtattttc tttgtaaaac aggtggtttg
     4321 aataggttaa tgttttttct gtttgatttt tttgtttatt ttttaaaatt ttctgccaaa
     4381 aaatacttta tataaataat tttatttcaa aattatattg tgtctgtttg ggtgtaatcc
     4441 gaggtaggcg tgctgcgggt gctttccttg tgtctgccgc tgctgttatg atgatatttt
     4501 tattctgtat ttaaggatgg aagatgagca gtccgcgcca tgtgttttac atttccgacc
     4561 gtaccggtct gactgccgag aatatcggcg aggctttgct gaaccagttt ggcaatctgt
     4621 cgttcaaacg ccatacgcat ccgtttgtcg atacgccgga aaaggcgcgc gcggtggtgg
     4681 agaaggtcaa tcggagccgg caggaaaacg gtcagcgtcc gattgcgttt gtcagtgtgg
     4741 ttgatgacga aatccgtcgg attatcaaag gggcggatgc ttttcagatt aatttctttg
     4801 agactttttt gggactgttg gagaaagaac tcaataccga agccacggca tccgagcagg
     4861 ggcatcacag tatcggtaat acgaagcgtt acgatgcgcg tatggaggcg gtgaatttct
     4921 cattgaacca cgatgacggg gtcagcgata agaaccttca ggaagcggat gtaatcttga
     4981 tgggtgtatc gcgttcgggc aaaacgccga cctgccttta cctcgccctg caatacggca
     5041 tccgtgcggc aaactatccg ctgattcccg acgatttgga atcggccgat ctgccgcgta
     5101 tggtcaagcc ttatagggat aagctgttcg ggttgaccat ccagccggaa cgtttgcagg
     5161 ccatccgcca agagcgccgc ccgaattcaa cttatgccaa aatcgataca tgccgcagcg
     5221 aggtggcaga cgcgcagagt atgttcagac ggcatggcat tccgtttgcg aatacgacgg
     5281 ataaatcagt ggaagagctt gccgtacaca tccttcaggc gtgcaagctc aaacgcaggt
     5341 tttgacgggc tttgattcgg tttgaaggcg gaaatgccgt ctgaaatcag gtttcagacg
     5401 gcagttttat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa
     5461 atagtacgga
//
LOCUS       DAXHSJ010000136         5458 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000136 DAXHSJ010000000
VERSION     DAXHSJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.136"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            294..1727
                     /locus_tag="ACFM8U_001689"
     CDS             294..1727
                     /locus_tag="ACFM8U_001689"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC6334893.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFLIKENKEALARLMTMEQGKGLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAVLKLGNGLDEGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIAVANDTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEVAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCLDVG"
     gene            1971..2759
                     /locus_tag="ACFM8U_001690"
     CDS             1971..2759
                     /locus_tag="ACFM8U_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC6334894.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVIEAFEKDRINIVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            3127..4683
                     /locus_tag="ACFM8U_001691"
     CDS             3127..4683
                     /locus_tag="ACFM8U_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC6334895.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4700..5446
                     /gene="surE"
                     /locus_tag="ACFM8U_001692"
     CDS             4700..5446
                     /gene="surE"
                     /locus_tag="ACFM8U_001692"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC6334896.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
BASE COUNT         1275 a         1369 c         1546 g         1268 t
ORIGIN      
        1 tttggatttt tgccatactc taccacacgt tgcaactgca atctttgttc cttatatatt
       61 attaaagggc tgcattactt ttctttctaa attaccattg tataaaaagt atttttctta
      121 aacgacaggg attgcgcccg cgccatattc agacaccgcc cggatgttgc gctgcccgat
      181 cggatgcttc agacggcatc ggaagggttt gcagttttgg aatatgaaga tgataatgtc
      241 cgcgagattg acggcatttg aaagcggtta ttccgcatac cggaggatac gaaatgaacg
      301 aatattccca attaatcaag catcccgata tttcccttcc cccggtttca gacggcatca
      361 aagtcgataa tccggcaacg ggcgagactt tggcgtttgt ccgcaagacg gattcggaca
      421 agctgaaaaa cctgattcaa aaggcggcgg cagcgcaaaa attatgggcg gcaaaaaccg
      481 cgttggaacg cgccgatgtg ttgtggcgtt ggtatttttt gataaaggaa aacaaagaag
      541 cactggcgcg tctcatgacg atggagcagg gcaaaggcct gaccgaggcg cgcggcgaaa
      601 tcgattatgc ggcttcgttt gtgcgctggt ttgccgaaga ggcgcggcgg attgacggcg
      661 atgtgctgac gagtgtgaaa gcgtcgcaaa aactggtcgt gttgaaacag cctgtcggtg
      721 ttaccgccgc gattacgccg tggaactttc cgtccgcgat gattgcgcgc aaggctgcgc
      781 cagctttggc agtgggttgc gcgatgatcg tcaaacccgc gtcgctcacg cctttgagcg
      841 cgtatgcgtt ggccgtgttg gcttacgaag cgggcgtgcc gcaggatttg ctgcctgttg
      901 tcagcggcag tgcttcggaa atcggccgtg aatttgccac gaacccgatt atccgcaaaa
      961 tcagcttcac cggttcgacc gaagtcggcg cgaaaatttt tgccgacagc gcggcggaca
     1021 ttaaaaaact cagtttggaa ctgggcggca atgcgccgtt tatcgtgttt gacgatgccg
     1081 atttggacaa agccgtcgaa ggcgcgctcg ccagcaagtt ccgcaacagc ggtcagacct
     1141 gtgtctgcac caaccgcgtt tacgctcaat cgggcattta cgacgcattt tgccgcaaat
     1201 taagtgaaaa agcagccgtg ctcaaattgg gcaacggctt ggacgaaggc gtgaaccaag
     1261 ggccgctgat tgaggaaaaa gcggtagaaa aagtcgagca gcacatcgcc gacgcactct
     1321 caaaaggcgc ggtctgtctg accggcggca aacgcagcgc gttgggcgga acgttttttg
     1381 agccgactgt cttaagcggc gtaacagcac aaatggcggt ggcgcgcgag gaaaccttcg
     1441 ggccgttgtg tccagtgttc cgttttgaaa ccgaagccga agtcatcgcg gtcgccaacg
     1501 atacggaata cggtttggca gcttaccttt tcaccgccga caccgcccgc caatggcgcg
     1561 tcggcgaagc attggaatac ggtatggtcg gcatcaatac gggcttaatc agtaatgaag
     1621 tggcaccgtt cggcggcgtg aaacgcagcg gtttgggacg tgaaggcagc aaatatggtg
     1681 cggacgaata tctagaattg aaatatttgt gtcttgatgt cgggtagagt atatatttga
     1741 tttatatgca aaaagttttt gggtatgtaa atctgataga ttcacatacc ccaacttata
     1801 ccccaattta tttgaagttg gtttgaagcc agtttgctac ggcttcttcg ttccaccggc
     1861 tgctcctgcc gtctgaacgg cagggtttca gacggcgccg cattttaagc agtctctatc
     1921 tgttgtacaa tgcgccctgt ttttacggtt atttttgatt tgaacaagat atgatggaaa
     1981 acggaaaaac agtcccgaga tggcgttttg ccttgaaaag tgcgggctgg cacctcttaa
     2041 tcagcctgtc ggttgcaggg ctggcggcat tgctggtttt taaggtttgg tatccttatc
     2101 cttatgccga gctgacggga gggctgtcgc tttatcagct ggtggtggct gtcgatattg
     2161 tatgcggtcc gctgctgact ttaattttgg caagcccgaa gaaaaagaca aaggcacgca
     2221 tggtcgattt ttccatggtc ggcatcatcc agctggcggc tttggcgtac ggtctgcaca
     2281 gcgtttcgct ggcgcgccct gtgatagagg cgtttgaaaa agacaggatt aatattgtaa
     2341 ccgccgccga agtggcggtt gaagatttgc acaaagcgcc ggaagggctg caaagcctgt
     2401 cgtggttcgg catccgccgc attgcattga aagaacctga ggatgcggat gagaagaaca
     2461 agacgctgga tttgtccctg aaaggtatcg agccgagtat gcgtcccgac cggtggctgc
     2521 cgtattccga caaggaagca gaagaaatcc gcaaacattt gaaaccgctg aaagtcttgg
     2581 cggatgcgag aaaaacgacg gttgcggaca ttctgaaaca ggcaggtctc gccgaagggg
     2641 aggagctgta ttacctgccg tttaccagca gcaggcagaa agagtggata gtcattaccg
     2701 ataaagaggg caacaccaaa ggctacgcgc cgatagacgg cttcatcatc accccttaag
     2761 cgttgggact ccgtccgcac tcgaacatcc gttcttcgcg gcggtagaat cagactgtat
     2821 ttgagagggg cgcaacccct caatcagggc aacacatagg ggaaggcttt atgtgccgtc
     2881 cgtgtgttga aacatttcaa aataagctca tgccgtccga acatcctttc agacggtatg
     2941 gcgttttgcc attgccccga taagctgtta aactatttaa attatttcga ccgaaggtac
     3001 acgcacatgc aagaccccga gcagagcagc aagcccgccc gctgtttttt gtgcgtaccg
     3061 tctgttcagg cagcttgagc gcttcaggct gccttttcgc gtttgaactt gaggaatacg
     3121 aaaatcatgg atttcagttg gttggcagaa ccgcatacct ggataggttt tgccacgctt
     3181 ttggtgttgg aagtcgtatt ggggatagac aatcttgtct ttgtggcgat tttggcaaac
     3241 aaggtccagc ccgcacggcg cgaccgcgca cggattatcg ggctggggct ggcagtcgtc
     3301 atccgcatca ttatgcttgc ttttatggcg cacatcatca cgctgaccga gccgctgttc
     3361 caaatcggcg gcctcgccgt ttccggcaag gacatgatta tgctcgcggg cggtattttc
     3421 ctgctttaca aagccaccac cgaactgcat gaacgcctcg aagggcacaa ccgttttacc
     3481 gttgccgaca accaaaaaaa acacgcgccg ttttggggcg tggtcgcgca aatcctgata
     3541 ctggatgccg tgttttccat cgattcggtc attactgcgg tggcgatggt cgatcatatc
     3601 gtcgtggcga tgggtgcggt cgtcgtcgcg atggctgtaa tgatttctgc cagcaaactc
     3661 ttgaccgaat ttgtcgacag acaccctacc gtcgtgatgc tctgccttgg ttttttgttg
     3721 atgatcggtt tcagcctgat tgccgaagcc ttccatttcc acattcccaa aggctacctc
     3781 tacgccgcca tcggcttctc gattttaatc gaactgttta accagatttc ccagcgcaac
     3841 agccgcaaaa acgactacat cggcagctcg tggcgcaagc gcaccgccga aaacgttttg
     3901 ggtatgatgg gcatacgcga aagcgtgctt gccgatgcgg gcggcgaatc cggggacgac
     3961 gcgcattttg aagaaaacga aaaatcgatg atacgcagcg tgctgacgct tgccgaacgc
     4021 ccgattatgg gggtgatgat cccacgccgc gacatcgaac ggctggacat ttcccaaagc
     4081 cgcgaagaac agtgtgccca actgcaaaat acgccttaca gccgcctgct cgttgtcggt
     4141 aaggcgggcg tggacgaacc tttgggctac atcaacaaaa aagacctgct gtcccaactg
     4201 ctggaaacag gcggtctcga cattcagacg gcattgcgcc agccactcgt cctgcccgac
     4261 agcaccaccg cgctgggcgc aatcgaactc ttccgccaaa gcagcgcgga ttatgctttg
     4321 gtggtggacg agttcggcgc ggtattgggc atggtaacca tgaaagacct gctcgaaacc
     4381 atcgcaggcg agttccccga agaatttgag cgcgaagaag aaccagccgt tcaggggaat
     4441 cccgatgaaa gcctgacggt ggaaggcgcg ttggaatatg tggagctggc atcccaactc
     4501 aatctgccgc agcaggagga agatgccgat ttccatacgg ttgccgggct gattatggaa
     4561 gaattgcaaa ccatccccga tgtcggcgat tttgccgatt tccacggctg gcggtttgaa
     4621 gtggtcgaaa aagaagggca gcgcatcgag cgggtcaaaa tcaccaaatt gcccgaagaa
     4681 taagcattca ggatagaaaa tgaacgtttt gatttccaac gacgacggct acctctccga
     4741 aggtattgcc gttttggcgc gcgttacggc ggaatttgcc aacgtcaggg tggttgcgcc
     4801 cgaacgcgac aggagcgggg tcagcaattc gctgacgctg gaacgccctt tgcagttgaa
     4861 acaggcgcaa aacgggttct actatgtcaa cggcacgccg accgactgta tccacatcgg
     4921 gcagtctgta ttttcggatt ttcaggccga ttttgtcttt tcgggcatca accggggcgc
     4981 gaatatgggg gacgacacgc tttattcggg gacggttgcg gcggcaaccg aagcctacct
     5041 tatgggcata cccgctgtgg cgttttcctt aaacgacgct tccggacgct attgggcgac
     5101 cgcagaacag gcactgtgga cattgttggc gcattttttc aaaaaccccc cgcagtcccc
     5161 tattttgtgg aacatcaata tccccgccgt tgcgccggaa gatgtgcggg gcattaaaat
     5221 cgcccgtttg ggcaggaggc atcacggtca gaacgtcatc cccgcgcgca atccgcgcgg
     5281 cgaacagatt tattggatag ggccggtcgg cgaagtttcc gatcgggaag agggaacgga
     5341 tttcggtgaa tgcggcgcag gtttcattac cgtaacgccg ttgcaaatcg acctgaccgc
     5401 ctatccggac atggcggaaa cggcggcgtt ctggcatacg gactgaccgt ttcatcaa
//
LOCUS       DAXHSJ010000137         5376 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000137 DAXHSJ010000000
VERSION     DAXHSJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.137"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(218..3049)
                     /gene="lbpA"
                     /locus_tag="ACFM8U_001693"
                     /gene_synonym="tbpA"
     CDS             complement(218..3049)
                     /gene="lbpA"
                     /locus_tag="ACFM8U_001693"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC6334897.1"
                     /translation="MNKKHGFQLTLTALAVATAFPAYAAQAGGATPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIQKQNKKAEYFLAEGEREP
                     KPVAKLAGNGNYLKNQLNRWVKERIEQNQPLSAEEEAMVREAQARHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDESKKF
                     SDKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISEYS
                     DYTDKGDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRQNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQ
                     KTKLPDSAGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYHKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3046..5244)
                     /locus_tag="ACFM8U_001694"
     CDS             complement(3046..5244)
                     /locus_tag="ACFM8U_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6334898.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAGPSIETTPVNRPAVGAAMRLLRRNIATSDKDGNDFPNSRQAEEKLSFKEG
                     DVLFLYGSKKDQRQQLEDEIRQRNPNVQIRTSENENKKYGYNFVDAGYVYVKGEDEIE
                     RTSNYKYFTHRFGYDGFVYYSGEHPSQSLPSAGTVKYFGNWQYMTDAIRHRTGKAGDP
                     SEDLGYLVYYGQNVGATSYAATADDREGEGKHPAEYTVDFDKKILEGKLIKNQYVQRK
                     TDKKNPLVIYDITAKLDGNRFTGSASVNPELKKSHSDKEYLFFHDSADQRLEGGFFGD
                     NGEELAGRFISNDKSVFGVFAGKQKTNASGTNPAMPSRKHTKILDSLKISVDEASGEN
                     PRPFEVSTMPDFGHPDKLLVEGREISLVNQEQIIQLADGRKMTVRACCDFLTYVKLGR
                     IKTERPASKPKAEDKREDEESAGVGNVEEGEGEVSEDEGEEAEEIVEEEPEEEAEEEE
                     AEPEEAEEESPTEESGSGSNAILPTPEAPKGRDIDLFLKGIRTAEADIPKNGTAHYTG
                     TWEARISTPIQWDNQADKTAKAEFNVDFGKKSISGTLTEKNGVEAAFHIENGVIEGNG
                     FHATARTRDNGINLSGTGSTNPKTFQASDLRVEGGFYGPQAAELGGTIFNKDGKSLGI
                     TEGTENKVEVEAEVEVEAETGVVEQLEPDEVKHQFGVVFGAKKDNKEVEK"
BASE COUNT         1148 a         1437 c         1253 g         1538 t
ORIGIN      
        1 ccgctctgcc gccattccca caaaagtggg aatccagttt ttcgagtttc agtcattccc
       61 gataaattgc cttagcattg aatgtctaga ttcccgcctg cgcgggaatg acgacggaaa
      121 tcttgtttat attgaatcaa aaaaacctgc accttaatca gttggcggtt tagtccgact
      181 tttggggtgc agatcaagct ttcagacggt atttccttta aaacttcatt tcgagcgcga
      241 ggctgaagtt cctgcccggt gcggcatacc ttccatagtt gctgtcgccg ccgtgccggt
      301 ttgccgtgct ttccgcggtc tggcgcaagg attcccaagt aacgtagcgg tagttgccga
      361 tattgtagat agccgccctc aaggtcagcc gttttttcag attcagatag gcggaaacgt
      421 ctgccgtcga ccaagaagac gacgctcttt ttgtcgaata tcgtttttga tcgcctgcca
      481 gataagcaag ctcgtcaggg tttttccctt tggaataggt cagcataatg tttgcccccc
      541 atttcccctc aggctggtcg tatccgaacc ccaaaacata acgcgacggc tgtaccgcat
      601 ccaaagcata gctgcggagg gatagtcccg gccgattgga taccgatttc ggtttgatgc
      661 ggttgtacgc caaagtcgtg tacaaacctt cgggcagttt gtgatacacg ccgttccagt
      721 cgatttttcc caagatgttg atgccttgga gcgacatatt ttgggcattg taataatcgc
      781 gtatatcaat ctctgtcaat cgtcctgccg aatccggcaa tttggttttt tgatcggcaa
      841 cggcgatcat atcggtatag cggttgcgga agctgctgat ttccaaaaag ccgaagtcgc
      901 ccttccactg caaaccgatt tcgcggttgg ctgccttttc cgatttcagg gcgggacgct
      961 gccagccttt cggataatcg tgataaatgt ctatcccgaa aagctcttgg aaggaaggcg
     1021 ttctgaagcc gctggaggcg cggtaagaca cggaaaaatg ccggttcggt ttgaacacga
     1081 tgccgctgtt ccacgaacgg tcaacatacc gcccgctgcg gacgagttct tccgacgtgg
     1141 taaagttttg ccggtcgtac ctgccgccca agctgaaatc gaaatatttg ccgattgaaa
     1201 aacggtcgtt caaagaaatg tggatattgc tgccgttgat ttttcttggc acgcatttgc
     1261 gggaacgcag ggtttcgatg tagccgcaga ccgacccttc gacgacttcg ggcttaccca
     1321 aaagatactt gtctccctta tcggtgtaat ccgagtattc cgaaatcctt gccgcattgt
     1381 gggaaagctg ttcggggcgg gagatcgctt tggaagcatc gtaaccgaag cccaaagtca
     1441 gatggtgttt cgtccatttg tttttcaggg atttttcaaa cgaggcattc aaaacattgt
     1501 gctgttcgcg gtagtggaaa cggtcgctgc tgtcgtagga atacggtttg tctgccgacg
     1561 cgcggcagga tttgtccaca gcaggataca cggcgcaatt cagcttcagc gtgttgttgt
     1621 cggttgccac gccctgtttg tcaaacgaca acaccgcctt atccgcccag ttgtccgagt
     1681 atttctcatt ttcataacga tacagcaaac ccatacggcg gcggcggtga tgttcgtcga
     1741 taaatttggt gcgggaatat ttcaaaccta tgcccttgac caaattttta tcgcctttcc
     1801 actcttctat attcggcaca aaatacaagc cgtcgcggaa atcgtcgccg tcgtacaccc
     1861 cgctcttgtc gctaaacttt ttcgactcgt ccgtaccgta atactgtttt tccgtcatat
     1921 cgcggatgtc gtaacgctgt ttggtatcct caaacacgcc gccgacataa tgcctgccgc
     1981 cgaagcggta gcccagcttg gcaagccaag agccgctgcg gtaatccatc ggatcgggca
     2041 atatcctgcc gccgcccgtg taagcttggg cggacaggtt ttcgtggcgc gcctgcgcct
     2101 cccgcaccat cgcttcttct tcagcactta aaggctgatt ttgttcaata cgttctttta
     2161 cccagcggtt gagctggttt ttcaaatagt ttccgttgcc cgctaatttt gccacgggct
     2221 taggttcacg ctcgccctcc gctaagaaat attccgcctt tttattctgc ttctgaatat
     2281 cgtatgtctg gcggaacgcg tccaaacggt ctatgccgta ttccaccccg tccgcaatat
     2341 cgccgtgcgg gcgcgtttcc cgcccttggc gttcggttcg gattagcagc ccttcccaac
     2401 cgtctttgct gaaccccgcg ccgagcgact tcataaattg gcggttttta ctgccgtagg
     2461 cagtttttgc ctgtatcccc cagcttttgc cgtctgaaat caggtctgcc gcttctttgg
     2521 tgcggaaggc gaccgcgccg ccgagtgcgc cgctgccgtg atcggacgaa ccggcacctt
     2581 tgtcgatttc caccgtgctg atgttttcat attcgatttc gttgattgca ccgctgccgc
     2641 cgcgtccgcc gtatccgctc aacgatccct gcacggtaaa cgcctgtatt tgggcaacgc
     2701 cgtcaaccga aaccgccaca cggtttttat ccacgccgcg tatcgagtag ccgccgctcg
     2761 cgccgttgcc ctgttcgaca accgccacgc ccggatcgta gcgcgtcagg tcgcggatac
     2821 cgagtacctg ttctttgttc aacgtttccg acgttttgac gattttgccc aaaccggtcg
     2881 cctctttcga tcgccgtccc actttggcgg cacggacggt aacctctttc agggattggg
     2941 tctgcgcggc atcaggtgtc gccccccccg cttgggcagc ataagccgga aaagcggttg
     3001 caacagccaa ggcagtcagg gtaagctgaa aaccgtgttt cttattcatt tttccacctc
     3061 tttattatct ttcttcgcac cgaataccac gccgaattgg tgtttaactt catcaggttc
     3121 taactgttcg acaacaccag tttcagcttc aacttcaact tcagcttcaa cttcaacttt
     3181 attttcagta ccttcagtta taccaagaga tttcccatcc ttattgaaaa tagtaccgcc
     3241 caattccgcc gcctgcgggc cgtaaaatcc tccttctaca cgaagatcac tagcttggaa
     3301 ggttttgggg ttggtcgaac cagttcccga aagattgatg ccgttatccc gagtgcgcgc
     3361 tgtcgcgtgg aaaccgttgc cctcaatcac gccgttttca atatggaaag cagcttctac
     3421 accgtttttc tccgtcagcg ttccggaaat cgatttcttg ccgaaatcaa cattaaactc
     3481 tgcttttgcg gttttatccg cctgattgtc ccattgaatg ggtgtgctga tacgcgcttc
     3541 ccaagtgccg gtataatgcg ccgttccgtt ttttggaatg tcggcttccg ccgtgcggat
     3601 acctttcagg aaaaggtcga tgtccctgcc tttaggggct tccggagtgg gcaggatggc
     3661 gtttgaaccg ctgccgcttt cttctgtcgg cgattcttct tcggcttctt cgggttcagc
     3721 ttcttcctct tcagcttctt cttcgggttc ttcttcgacg atttcttcgg cttcttcgcc
     3781 ttcatcttcg gaaacttcgc cttcgccttc ttcgacgtta ccaacgcctg cactctcttc
     3841 atcctccctt ttatcttccg cctttggttt acttgccggg cgttcggttt ttatccgtcc
     3901 gagttttaca taggtcagaa aatcgcaaca agcacggacg gtcattttcc tgccgtcggc
     3961 aagctggatg atttgctctt ggtttaccaa ggagatttca cgcccttcga caagaagttt
     4021 gtcgggatga ccaaaatcgg gcatagtgga aacctcaaac ggtcggggat tttcaccact
     4081 tgcctcatca acggaaattt tcagagaatc caagattttg gtgtgttttc tagacggcat
     4141 agcaggattt gtgcctgatg cgtttgtttt ttgtttgcct gcgaatacgc cgaatacgct
     4201 tttgtcgttg ctgataaacc gtccggcaag ctcttctccg ttatcgccga aaaaaccgcc
     4261 ctcaagccgc tgatcggcac tgtcatggaa aaacaaatat tccttgtccg aatggctttt
     4321 cttcaattca ggattgacac tggcactgcc ggtaaagcgg ttgccgtcca attttgcagt
     4381 gatgtcgtaa atgaccagcg ggttcttttt atcggttttc ctttgcacat actgattttt
     4441 aatcagcttg ccctccagga tttttttgtc gaaatcaacc gtatattcgg caggatgttt
     4501 tccctccccc tcccggtcgt cggcagtcgc agcataagaa gttgctccga cattttgacc
     4561 gtaataaacg agataaccca aatcttcgct aggatctcct gcttttcctg tccgatgacg
     4621 tatggcatcg gtcatatatt gccagttacc gaaatatttt accgttcccg cgctcggtaa
     4681 agattgcgaa ggatgttctc cggaataata tacaaaacca tcataaccaa accggtgagt
     4741 aaaatacttg taatttgaag tcctctcaat ttcatcttct ccctttacat atacataacc
     4801 tgcatctaca aagttataac catatttttt attttcattt tctgatgtcc taatttgtac
     4861 attaggattg cgttgacgaa tttcatcttc aagctgctga cgttgatctt ttttggaacc
     4921 gtataaaaac agaacatcac cttctttaaa cgacagcttt tcttctgctt gtctgctatt
     4981 tggaaaatca ttgccatcct tatcagaagt tgcgatattc cgcctcaaca gccgcattgc
     5041 cgcaccgacg gcgggccggt tgaccggcgt ggtttctata gaaggcccgg cagggggggg
     5101 agtgggaacg tccttagact tgaaagtgac ggggtacgcg gtcggcgttg attcgacaac
     5161 aggctgcacg ccgaaattgc cgccgataca agatgctaaa agtaagggca acaagacaat
     5221 gccgccataa ttcggtttac acatccctac ttttcctcct atttgattaa taataattat
     5281 cattatatta atatgtacag ataatatcaa gccgttttta tagtgaatta acaaaaatcc
     5341 ggacaaggcg acgagccgca gacagtacag atacat
//
LOCUS       DAXHSJ010000138         5272 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000138 DAXHSJ010000000
VERSION     DAXHSJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.138"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            122..817
                     /gene="pgl"
                     /locus_tag="ACFM8U_001695"
     CDS             122..817
                     /gene="pgl"
                     /locus_tag="ACFM8U_001695"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC6334899.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            798..1784
                     /locus_tag="ACFM8U_001696"
     CDS             798..1784
                     /locus_tag="ACFM8U_001696"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC6334900.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1834..2682
                     /gene="hexR"
                     /locus_tag="ACFM8U_001697"
     CDS             1834..2682
                     /gene="hexR"
                     /locus_tag="ACFM8U_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC6334901.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2803..4449
                     /gene="pgi"
                     /locus_tag="ACFM8U_001698"
     CDS             2803..4449
                     /gene="pgi"
                     /locus_tag="ACFM8U_001698"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6334902.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGSQKHDSSTERLINLYLQTNRK"
     gene            4592..5014
                     /locus_tag="ACFM8U_001699"
     CDS             4592..5014
                     /locus_tag="ACFM8U_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6334903.1"
                     /translation="MALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEE
                     RYKLSRSEFEQLWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSEL
                     ACKTVETEARLEELYNRKKALIDEMVREADKKELPKRL"
BASE COUNT         1343 a         1469 c         1394 g         1066 t
ORIGIN      
        1 ccactataaa aagcagtccc gatatttggt atcaaaagca taaacaactg ttcggccggc
       61 atattgctca acccatttcc ataacggaat atgccgtctg aaataaaaaa aggacacaaa
      121 tatgtttgtt tggcacgaat acgaaaatgc ggcagaagcg gcgcagtctt tggctgacgc
      181 agtggcggat gctttgcagg gcgcactgga cgagaagggc ggtgcggtgt tggcagtttc
      241 cggcggacgt tcgccgattg catttttcaa cgccctgtcg caaaaagatt tggattggaa
      301 aaacgtcggc atcaccttgg cagatgaacg catcgtgccg accgtccacg ccgacagcaa
      361 taccggtttg gtgcgcgaat acctgttgaa gaacaaagcg gaagcggcaa tgtggattcc
      421 tatggtggaa gacggaaaaa ctgaaaccga attacatccc gatgctgttg tcgattatgc
      481 actgaaacat tacaaacagc ccgatgtttt ggttttgggt atgggaaacg acgggcatac
      541 ggcttcgatt ttcccgaaag ctccgcagtt tcagacggca atcgacggtt cggcaggtgt
      601 cgcgctggtg cataccacgc ccgttaccgc gccgcacgag cgcgtcagta tgaccttgga
      661 tgcgattgcc catacggggc atgtgttttt ggcgatacgg ggcgaagaga aaaaagccgt
      721 gttcgaccaa gccgcacaag gcgaaaaccg cgaatatccg atcaacctcg ttttgaacca
      781 tcaaggagtg aactgccatg tcttctacgc cgaataaaca agccggatat ccccgactgg
      841 tcgccgatat cggcgggacg aatgcacgct ttgcgctgga aaccgcgccg cgcgtcattg
      901 aaaaagccgc cgtgcttccg tgtaaagact acgatacggt taccgatgcg gtgcgtgcct
      961 atctgaatca aagcggtgca acagccgtac ggcacgcggc atttgccatc gccaacccga
     1021 ttttgggcga ctgggtgcag atgaccaacc accattgggc gttttccatc gaaaccaccc
     1081 gtcagacttt ggggctggac accctcatcc ttttgaacga ctttaccgcg caggcattgg
     1141 cggtaacgca gacttcaagc aaagacctga tgcaggtagg cgggcaaaag cctgtcgaat
     1201 ttgcccccaa agccgttatc ggccccggta ccggcctggg cgtgagcgga ttggtgcaca
     1261 gccacgcagg ctgggtggct ttggcaggcg agggcgggca taccagtttc ccgccgtttg
     1321 acgatatgga agtgctgatt tggcagtacg ccaaaaacaa atacggccat gtttccgccg
     1381 aacgcttttt gagcggcgcg ggcttgagct tggtttacga ggctttggct gcaaaacaga
     1441 aagccaaacc cgccaaactg atgccgtctg aaatcacgga aaaggctttg agcggcgcgt
     1501 cgcctttgtg ccgtcagact ttggacatct tctgcgccat gctcggcacg gttgcttcca
     1561 acctcgccct gacgctgggt gcgcgcggcg gcgtgtacct gtgtggcggc attattcccc
     1621 gcgtgttgga atatttcaaa acttccccgt tccgcagccg tttcgagaac aagggcaggt
     1681 ttgaagcata tcttgccgcg attcccgtgt atgtcgtctt gagcgagttt cccggaattt
     1741 ccggtgcggc tgcggctctc gacaaccatt tgagaaacgt ttaaccacag cggctccttg
     1801 cagcggggct gcattatcga agggcatatc attatgttaa gcaaaatcag cgaatcactg
     1861 gcaaaccttt ccggtgcgga acgcaaagtc gccgaatgtg cattggcgga acccaaatgg
     1921 ttcgtccatg ccgccgttgc cgaaattgcc gaacgtgcgt ccgtcagcca gccgaccgtc
     1981 atccgattct gccgcagctt gggttataaa gggctgcccg agttcaagct cgccttgtcc
     2041 gccagcatcg gtcatgaggg tatgccctat gtccacgaag aactcaacgc cgacgacgat
     2101 atggcaagcg tggtcgagaa agtattgggc aatgccgccg cctcgctgtt gggcgaacgc
     2161 cgcttcctga aagagtcgga gctggaaaac gccattgcca cgctgatgca cgcccgccgc
     2221 gtcgagtttt acggtgtcgg caattccggt atcgtcgcgc aggacgcgca gcacaagttt
     2281 ttccgtttcg gaatgtccac ggtcgcctat gtcgatacgc acacgcagct gatggcggca
     2341 tccgttttga gcgatcagga tgttttggtt gccatttcca acacgggttc gtctatcgaa
     2401 cttttggatg cggtcagcat cgccaaagaa aacggcgcgt ccgtcatcgc actgacccgc
     2461 aacgattcgc ctctggcgca acttgccgac tgcgtgttga gcgttgccac acaggaaaat
     2521 gccgaactct acacgcccat ggtttcccgc ctcttgcagc ttgccgtcat cgacatcctt
     2581 gccatcggac ttgccctgcg cttgggcgat gctgccagcc tgcaattgca gaaaagcaaa
     2641 aaaagcatac acaacaagca catcgattac gacaaagatt gaccttcaga cggcatccca
     2701 caaatgccgt ctgaaatgcc gaacaacggt cgtcggcggc ttgcggcagt ttccggcagc
     2761 cttttcatcc cacaacaaaa aacctcattc aggagcatat agatgaaaca ccttcacgac
     2821 ttacccgcat ggtcgaaatt gtggaaccac tttgacgaca gcaaaacatt gcatatgcgc
     2881 gaaatgttcg agcaagaccc gcagcgtgcg gaacgctact ggctgcaagt cggcggactg
     2941 acgctggact actccaaaaa ccgcatcaac gacgaaacca tgtcgctttt gttcgagctt
     3001 gcccgagaag caggcgtgcc ggagcggatg cggcagatgt tccacggcga aaaaatcaat
     3061 accaccgaaa accgcgccgt cctgcatgtc gcccttcgca accgcaccaa ttcgccgatt
     3121 gtggttgacg gtgaagatgt gatgcccaaa gtcaaccgcg ttttgcaacg tatgggcgaa
     3181 tttgcacacg aagtccgcag cggaagctgg ctgggctata ccaaccaagt cattaccgac
     3241 gttgtcaaca tcggcatcgg cggatcggat ttgggtccgc tgatgatgtg taccgcgctc
     3301 aaacctttcg gtcatccgcg cctcaatatg cacttcgtct ccaacgtgga cggctcgcaa
     3361 ctgcgcgacg tattgtccaa agtccacccc gaaaccacgc tgttcatcat cgcctccaaa
     3421 acatttacca cgcaggaaac gctgaccaac gcgctgaccg cgcgcgaatg gtttttgaat
     3481 catgcgggcg acgaagaagc cgttgccaaa cacttcgccg ccgtttccac caatcaaaaa
     3541 gccgtcgccg aattcggcat cgacaccgcc aatatgtttg aattttggga ttgggtcggc
     3601 ggtcggtaca gcctgtggtc cgccatcgga ttgccgatta tgctgtatct cggcgaagaa
     3661 aacttcatcg aaatgctcaa cggcgcgcac ctgatggacc aacacttcat caacacaccg
     3721 ctcgagcgca acctgcccgt cattctcgcc ctcatcggca tctggtatat caactactac
     3781 ggcggcggca gccacgtcat cgcgccttac gaccaacatt tgcaccgcct gcccaaattc
     3841 atccagcagc tcgatatgga aagtaacggc aaacaggtta cgttggacgg caaagcagtc
     3901 ggacacgaaa cctcgccgat tatctggggc gaaacgggca ttaacggcca gcacgccttc
     3961 ttccaactgc tgcaccaagg cacgcacatt acccccatcg acctgattgc ctcgcttgaa
     4021 aaacgcagca acctgcctgg acaccacgaa atcctgcttg ccaacgtctt cgcccaagca
     4081 gaagccttta tgcgcggcaa aacccccgac gaagtccgcg ccgaactcaa agcgcagggt
     4141 atggatgagg cgcgcatcga agagctggtc ccgcacaaaa ccttctccgg taaccgcccg
     4201 accaacctca tcctgatgga caaggtcaac ccgcgtaata tgggcagcct gattgccatg
     4261 tacgaacaca aaaccttcgt acaaggcatc atttggggca tcaacagctt cgaccagtgg
     4321 ggcgtggaac tcggcaaaca actggctaaa accattttgg gcgaactgac cggcgaaacc
     4381 gggtcgcaaa agcacgacag ctcgaccgaa cgcctgatca acctctacct gcagaccaac
     4441 cgcaaataaa acctgcggaa aaatgccgtc tgaacgccga ccgtttcaaa cggcattttt
     4501 atcgaacagg aaaaccgtcg gtaaacttgc agagcgtgtg caatcccgat atgatggttt
     4561 gcataaattt aaacatatat gttccgcagc tatggcactg attaaagagc cgttggacaa
     4621 agcgaaacaa aggaacgaag aacttgaagc ggcagaagaa gcggcggcgc aggaggcatt
     4681 gggtcgggag caggaagtcg accgcgtatc cgaatgggaa gaacgctaca agctgtcgcg
     4741 cagcgagttc gagcagctct ggaaaggatt gcctcaaacc gtacagaata agctgcaagc
     4801 ctcacagaaa acatggaaaa gcggtatgga caaaatctgt gccaacaatg cgaaagccga
     4861 aggtgaaacg ccaaacggca taaaagtcag cgaactggca tgcaaaacgg tggaaaccga
     4921 agcacgcttg gaagagctgt acaaccgtaa aaaagccctt atcgacgaaa tggtcaggga
     4981 agcggacaag aaagaactgc caaagcggct ctgaacagcg cggttcaggc actgcccgcc
     5041 gatattgccg aaaccgtcat gcccgagtac cgcaactgac aaaacggttt gaatgccaaa
     5101 tgcgccgacg gcgacgaata cggcgtggca caatctgact gccgtaccag agaaatcaat
     5161 gcgaaaacca aagaaatcca aggttatctg attgactgaa acttggatgc gggaatgtcg
     5221 gcggcttttg cgtttttgtc gtttttatag tgaattaaat ttaaaccgat ac
//
LOCUS       DAXHSJ010000139         5247 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000139 DAXHSJ010000000
VERSION     DAXHSJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.139"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            176..2314
                     /locus_tag="ACFM8U_001700"
     CDS             176..2314
                     /locus_tag="ACFM8U_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6334904.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKTASPKDQGGYGFAMRLKRRNWYPSAKENEVKLNEGDWEATGLPTEPKKLPL
                     KQESVISKVEANNGDNNIYTSPYLTQSNHQNGSANQPKNEVKDYKEFKYVYSGWFYKH
                     AKLEIIKENNLIKGAKSGDDGYIFYHGEKPSRQLPVSGEVTYKGVWHFVTDTKQGQKF
                     NDILETSKGQGDKYSGFSGDEGETTSNRTDSNLNGNHEGYGFTSNLEVDFDNKKLTGK
                     LIRNNRVTNATTGDKHTTQYYSLEAQVTGNRFNGKAMATDKPGNGETKQHPFVSDSSS
                     LSGGFFGPKGEELGFRFLSDDKKVAVVGSAKTKDKDANGNTEAASGGTGAAASGGAAA
                     MPSENGKLTTVLDAVELTLNDKKIKNLDNFSNAAQLVVDGIMIPLLPEASESGNNQAN
                     QGTNGGTAFTRKFDHTPKSDEKDTQAGTAANGNQAASNTAGDANGKTKTYAVEVCCSN
                     LNYLKYGLLTRKTAGNTGEGGNGSPTAAAQTAQGAQSMFLQGERTDEKEIPSEQNVVY
                     RGSWYGHIANSTSWSGNASNATSGNKADFTVNFGEKKINGTLTAENRQAATFTIEGTI
                     QGNGFSGTAKTADSGFDLDQSNTTGTPKAYITNAKVQGGFYGPKAEELGGWFAYPGDK
                     QAQPPASGSGASAANSATVVFGAKRQQLVQ"
     gene            2401..5148
                     /gene="tbpA"
                     /locus_tag="ACFM8U_001701"
     CDS             2401..5148
                     /gene="tbpA"
                     /locus_tag="ACFM8U_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HFC6334905.1"
                     /translation="MQQQYLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEAAGRGVQSFNRLVPVEDDNQYAHFVVEEECESGSYETCKSKP
                     KKDVVGKDERQMVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKAVFDENKKQAGSLSGNGKYAGDHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     DLRHQDYFYQNANRAYSSKIPTQNGNQNISPNGSQDKPYWVSIGGGNVVTGQICRLGN
                     NTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTL
                     SWNAGIVLKPADWLDLTYRTSTGFRLPSFSEMYGWRSGDKIKAVKIDPEKSFNKEAGI
                     VFKGDFGNLEASWFNNAYRDLIVRGYEAQIKNGKEEAKGDPAYLNAQSARITGINILG
                     KIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYD
                     QPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTI
                     KKHFTLRAGVYNLLNHRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMK
                     F"
BASE COUNT         1504 a         1340 c         1341 g         1062 t
ORIGIN      
        1 gatatacgtg ggaatgacgg gatataggtt tccgtgcgga cgcgttcgga ttcccgcctg
       61 cgcggaaatg acgggatttt ggtgtattcc ctaaaaaaat aaaaaagtat ttgcaaattt
      121 gttaaaaata aataaaataa taatccttat cattctttaa ttgaattggg tttatatgaa
      181 caatccattg gtaaatcagg ctgctatggt gctgcctgtg tttttgttga gtgcttgttt
      241 gggcggaggc ggcagtttcg atcttgattc tgtcgatacc gaagccccgc gtcccgcgcc
      301 aaagtatcaa gatgtttctt ccgaaaaaac cgcaagccca aaagaccaag gcggatacgg
      361 ttttgcgatg aggttgaaac ggaggaattg gtatccgtcg gcaaaagaaa acgaggttaa
      421 actgaacgag ggtgattggg aggcgacagg attgccgaca gaacccaaga aactgccatt
      481 aaaacaagaa tccgtcattt caaaagtaga agcaaacaat ggcgacaaca atatttacac
      541 ttccccctat ctcacgcaat caaaccatca aaacggcagt gcaaaccaac caaaaaatga
      601 agtaaaagat tataaagagt tcaaatatgt ttattccggt tggttttaca aacacgctaa
      661 actcgaaatc ataaaagaaa acaacttaat taagggtgca aagagcggcg acgacggtta
      721 tatcttttat cacggtgaaa aaccttcccg acaacttccc gtttctggag aagttaccta
      781 caaaggtgtg tggcattttg taaccgatac gaaacaggga caaaaattta acgatattct
      841 tgaaacctca aaagggcaag gcgacaaata tagcggattt tcgggcgatg aaggcgaaac
      901 aacttccaat agaactgatt ccaaccttaa tgggaatcac gagggttatg gttttacctc
      961 gaatttagaa gtggatttcg acaataagaa attgacgggc aaactgatac gcaataatag
     1021 agttacaaac gctactactg gcgacaaaca taccacgcaa tattacagcc ttgaggctca
     1081 agtaacaggc aaccgcttca acggcaaggc gatggcaacc gacaaacccg gcaacggcga
     1141 aaccaaacaa catccctttg tttccgactc gtcttctttg agcggcggct ttttcggccc
     1201 gaagggtgag gaattgggtt tccgcttttt gagcgacgat aaaaaagttg cggttgtcgg
     1261 cagcgcgaaa accaaagaca aagacgcaaa tggcaatact gaggcggctt caggcggcac
     1321 aggtgcggca gcatcgggcg gtgcggcagc tatgccgtct gaaaacggta agctgaccac
     1381 ggttttggat gcggttgaat tgacactaaa cgacaagaaa atcaaaaatc tcgacaactt
     1441 cagcaacgcc gcccaactgg ttgtcgacgg cattatgatt ccgctcctgc ccgaggcttc
     1501 cgaaagtggg aacaatcaag ccaatcaagg tacaaacggc ggaacagcct ttacccgcaa
     1561 atttgaccac acgccgaaaa gcgatgaaaa agacacccaa gcaggtacgg cggcgaatgg
     1621 caatcaagcc gcttcaaata cggcaggtga tgccaatggc aaaacaaaaa cctatgcagt
     1681 cgaagtctgc tgttccaacc tcaattatct gaaatacggg ttgctgacac gcaaaaccgc
     1741 cggcaacacg ggggaaggcg gcaacggcag cccaaccgcc gccgcccaaa cggcacaggg
     1801 cgcacaaagt atgttcctcc aaggcgagcg caccgatgaa aaagagattc caagcgagca
     1861 aaacgtcgtt tatcgggggt cttggtacgg gcatattgcc aacagcacaa gctggagcgg
     1921 caatgcttcc aatgcaacga gtggcaacaa ggcggacttt accgtgaatt ttggcgagaa
     1981 aaaaattaac ggaacgttaa ccgctgaaaa caggcaggcg gcaaccttta ccattgaggg
     2041 aacgattcag ggcaacggtt tttccggtac ggcaaaaact gctgactcag gctttgatct
     2101 cgatcaaagc aataccaccg gcacgcctaa ggcatatatc acaaacgcca aggtgcaggg
     2161 cggtttttac gggcctaaag ccgaagagtt gggcggatgg tttgcctatc caggcgacaa
     2221 gcaggcgcag ccgcccgctt cggggtcggg cgcatcagcc gccaacagcg cgaccgtggt
     2281 attcggtgcg aaacgccaac agcttgtgca ataagcacgg ctgccgaaca atcgagaata
     2341 aggcttcaga cggcatcgct ccttccgatg ccgtctgaaa gcgaagagta gggaaacact
     2401 atgcaacagc aatatttgtt ccgattaaat attttatgcc tgtcgctgat gaccgcgctg
     2461 cccgtttatg cagaaaatgt gcaagccgga caagcacagg aaaaacagtt ggataccata
     2521 caggtaaaag ccaaaaaaca gaaaacccgc cgcgataacg aagtaaccgg tttgggcaaa
     2581 ttggtcaaaa ccgccgacac cctcagcaag gaacaggtac tcgatatccg cgacctgacg
     2641 cgttacgacc ccggcatcgc cgtcgtcgaa caggggcgcg gcgcaagctc tggctactcg
     2701 atacgcggta tggacaaaaa ccgcgtttcc ttgacggtag acggcttggc gcaaatacag
     2761 tcctacaccg ctcaggcggc attgggcggg acgaggacgg cgggcagcag cggcgcaatc
     2821 aatgaaatcg agtatgaaaa cgtcaaggct gtcgaaatca gcaaaggctc aaactcggtc
     2881 gaacaaggca gcggcgcatt ggcgggttcg gtcgcatttc aaaccaaaac cgccgacgat
     2941 gttatcgggg aaggcaggca gtggggcatt cagagtaaaa ccgcctattc cggcaaaaac
     3001 cgggggctta cccaatccat cgcgctggcg gggcgcatcg gcggtgcgga ggctttgctg
     3061 atccacaccg gccggcgcgc gggggaaatc cgcgcccacg aagccgccgg acgcggcgtt
     3121 cagagcttta acaggctggt gccggttgaa gatgacaacc aatacgctca tttcgtggtc
     3181 gaagaagaat gcgaaagcgg gagttatgaa acgtgtaaaa gcaaaccgaa aaaagatgtt
     3241 gtcggcaaag acgaacgtca aatggtttct acccgagact acacgggtcc caaccgcttc
     3301 ctcgccgatc cgctttcata cgaaagccgg tcgtggctgt tccgcccggg ttttcgtttt
     3361 gaaaacaaac ggcactacat cggcggcata ctcgaacgca cgcaacaaac tttcgacacg
     3421 cgcgatatga cggttccggc attcctgacc aaggcggttt ttgatgaaaa taaaaaacag
     3481 gcgggttctt tgtccggcaa cggcaaatac gcgggcgacc acaaatacgg cggtctgttt
     3541 accaacggcg aaaacggtgc gctggtgggc gcggaatacg gtacgggcgt gttttacgac
     3601 gagacgcaca ccaaaagccg ctacggtttg gaatatgtct ataccaatgc cgataaagac
     3661 acttgggcag attatgcccg cctctcttac gaccgtcagg gcatcggttt ggacaatcat
     3721 tttcagcaga cgcactgttc tgccgacggt tcggacaaat attgccgccc gagtgccgac
     3781 aagccgtttt cctactacaa atccgatcgc gtgatttacg gggaaagcca caggctcttg
     3841 caggcggcat tcaaaaaatc cttcgatacc gccaaaatcc gccacaacct gagcgtcaac
     3901 ctcggttttg accgttttgg ttccgacctc cgccatcagg attattttta tcaaaatgcc
     3961 aaccgcgcct attcgtcgaa aatacccact caaaacggca atcaaaacat cagccccaac
     4021 ggcagccaag acaaacccta ttgggtcagc ataggcgggg gaaatgtggt tacggggcaa
     4081 atttgccgct tgggcaacaa tacttatacg gactgcacgc cgcgcagcat caacggcaaa
     4141 agctattacg cggcagttcg ggacaatgtc cgtttgggca ggtgggcgga tgtcggcgcg
     4201 ggcttgcgct acgactaccg cagcacgcat tcggacgacg gcagcgtttc caccggaacg
     4261 caccgcaccc tgtcctggaa cgcaggcatc gtcctcaaac ctgccgactg gctggatttg
     4321 acttaccgca cttcaaccgg cttccgcctg ccctcgtttt cggaaatgta cggctggcgg
     4381 tcgggcgata aaataaaagc cgtcaaaatc gatccggaaa aatcgttcaa caaagaagcc
     4441 ggcatcgtgt ttaaaggcga tttcggcaac ttggaggcaa gttggttcaa caatgcctac
     4501 cgcgatttga ttgtcagggg ttatgaagcg caaattaaaa acggcaaaga agaagccaaa
     4561 ggcgacccgg cttatctcaa tgcccaaagc gctcggatta ccggcatcaa tattttgggc
     4621 aaaatcgatt ggaacggcgt atgggataaa ttgcccgaag gttggtattc tacatttgcc
     4681 tataatcgtg tccgtgtccg cgacatcaaa aaacgcgcag accgcaccga tattcaatca
     4741 catctgtttg atgccatcca accttcgcgc tatgtcgtcg gcttgggcta tgaccaaccg
     4801 gaaggcaaat ggggtgtgaa cggtatgctg acttattcca aagccaagga aatcacagag
     4861 ttgttgggca gccgggcttt gctcaacggc aacagccgca atacaaaagc caccgcgcgc
     4921 cgtacccgcc cttggtatat tgtggatgtg tccggttatt acacgattaa aaaacacttc
     4981 accctccgtg cgggcgtgta caacctcctc aaccaccgct atgttacttg ggaaaatgtg
     5041 cggcaaactg ccggcggcgc agtcaatcaa cacaaaaatg tcggcgttta caaccgatat
     5101 gccgcccccg gccgaaacta cacatttagc ttggaaatga agttctaaac accccctcgc
     5161 cgcaaatgcc gtctgaaagg cttcagacgg cattttttac acaattccca ccattttcca
     5221 tcatccccga tacaccgtaa tctcgaa
//
LOCUS       DAXHSJ010000140         5207 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000140 DAXHSJ010000000
VERSION     DAXHSJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.140"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(72..446)
                     /locus_tag="ACFM8U_001702"
     CDS             complement(72..446)
                     /locus_tag="ACFM8U_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6334906.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVTYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
     gene            complement(443..571)
                     /locus_tag="ACFM8U_001703"
     CDS             complement(443..571)
                     /locus_tag="ACFM8U_001703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334907.1"
                     /translation="MPQTWGEAIQFRIKKQIENELAPPNWSTQFPNGSIYDPKVTK"
     gene            complement(619..975)
                     /locus_tag="ACFM8U_001704"
     CDS             complement(619..975)
                     /locus_tag="ACFM8U_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC6334908.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(980..1084)
                     /locus_tag="ACFM8U_001705"
     CDS             complement(980..1084)
                     /locus_tag="ACFM8U_001705"
                     /inference="COORDINATES: protein motif:HMM:NF018014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334909.1"
                     /translation="MDDIRGIIQGAVNPLIYKVSKQKIIYNNKLISGT"
     gene            complement(1118..1462)
                     /locus_tag="ACFM8U_001706"
     CDS             complement(1118..1462)
                     /locus_tag="ACFM8U_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334910.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(1621..2022)
                     /locus_tag="ACFM8U_001707"
     CDS             complement(1621..2022)
                     /locus_tag="ACFM8U_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334911.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(2026..2478)
                     /locus_tag="ACFM8U_001708"
     CDS             complement(2026..2478)
                     /locus_tag="ACFM8U_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334912.1"
                     /translation="MQTKALGTVDEIGDTVQQVGKQASGQKTSGGNPAIDSDPYSPSS
                     VAARIEAGKARSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDFNSLNVRNVQ
                     TRPNGTITGNLPDGRAVNARNDSSGGEPTLEITISNNRKIKIRYGNTR"
     gene            complement(2473..2766)
                     /locus_tag="ACFM8U_001709"
                     /pseudo
     CDS             complement(2473..2766)
                     /locus_tag="ACFM8U_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(2836..3153)
                     /locus_tag="ACFM8U_001710"
     CDS             complement(2836..3153)
                     /locus_tag="ACFM8U_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334913.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(3150..3410)
                     /locus_tag="ACFM8U_001711"
     CDS             complement(3150..3410)
                     /locus_tag="ACFM8U_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6334914.1"
                     /translation="MRPTTRDELRQALQEQGFRRTGSDEAQYETWKGPDGVKIDIRPN
                     GEVIRTQRVPRTDGVQGKYPQRQNYEGNPLPNNRHHSGYFIK"
     gene            complement(3408..3752)
                     /locus_tag="ACFM8U_001712"
                     /pseudo
     CDS             complement(3408..3752)
                     /locus_tag="ACFM8U_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(4178..4522)
                     /locus_tag="ACFM8U_001713"
     CDS             complement(4178..4522)
                     /locus_tag="ACFM8U_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334915.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(4560..4883)
                     /locus_tag="ACFM8U_001714"
     CDS             complement(4560..4883)
                     /locus_tag="ACFM8U_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334916.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYETFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(4886..>5207)
                     /locus_tag="ACFM8U_001715"
     CDS             complement(4886..>5207)
                     /locus_tag="ACFM8U_001715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334917.1"
                     /translation="PAVRPMQTVDGEMAGGNKSLKIGTQSVEKSTGRTIPNNLKEQLA
                     MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEIHYIENSRTGEKTDF
                     KFKD"
BASE COUNT         1532 a          984 c          783 g         1908 t
ORIGIN      
        1 tttaaaacaa ttagtaaatc gagtttatcc tagttatcca ggatggtccc atctctttta
       61 tagatagctg cttaccaagt atccttaatt gctaattcaa gccactcttc aaaactatta
      121 aaaaattcat ggttttctat attatctaaa ttaatgacaa taaccctagg atttcctgtt
      181 acatcattac catcaaaata agcttcccat tctccattac tagcgaatgg gataagattt
      241 aaatcaggca ggatagaatt tctaaatata taagttactt ctgccccttc tgttccataa
      301 ttctcaaacc accagtgaaa attatgtggc tgaatataag gtattaaaga tgtatcgtta
      361 gagatacgaa catacttttc aggataacaa aacctttcag gaagcatatt actaggatat
      421 aaattaaatt cattttgaat aatcatttcg ttaccttagg atcataaata ctaccattag
      481 gaaactgggt agaccaattt ggtggtgcta gttcattttc aatttgtttt ttaattctaa
      541 attgaatagc ttctccccat gtttgcggca tctaatgctg cttgctctgc atcaatatta
      601 ctaaaaattg gattagtctc aaaaatgaaa aattactgtt ttttcatttt ttatagcata
      661 ttcaagtaca tgaataatat ttttcactaa gggctctgta gaaaaatctg aattttgtaa
      721 tttttctttt acagccaaaa tactttcctt atctattagc caatccccct cacttggtcc
      781 aaataaccaa ttatcggtaa tttcagaaac aagtctccat aatccacgat tttgatattc
      841 aataatttga ttttccgtaa tttctacaac tgtttcagat tgaatgtttt tataagccca
      901 atcaatcatt tcagtatatt cactaattga aacattgtct tttaaacaga aaattaacag
      961 gttgtgttca ttcatttgtc taagttcctg atatgagttt gttgttgtaa ataatctttt
     1021 gcttgcttac cttgtaaatt aaaggattaa ccgcaccttg gatgatgccg cgaatatcat
     1081 ccatacggtt accccaccaa tttttattgg tgtatcgtta atcatttatt tcaaaaaagt
     1141 tatttattat catattatca gggagcaggt cgttaaaaaa aaccccctca aattccatta
     1201 aattagtatt tatccagcac ataaacgaac caaagcgttc tttccctgag tgtaaatatt
     1261 taaaattaaa ctcaaaataa gtttttttgc ttagaaaatt taatggtttt tctatagtga
     1321 aaaaaaagcc cttactcgtt ttcgttcgag atactatttt aaataaattt ttattttcaa
     1381 taaaataaga atattcattt actaagttgt tatgataaaa aagtaatttt attaaaatgt
     1441 tttcataatt ttctaggttc atttctagct caatttaaaa tgatttcatt gttgccgtac
     1501 caatgcgaaa tttaccatca acaaaatttc ccttcactga tacaggtaca ccatcaactt
     1561 gggtagtttt attttcaggc tgcctgaaag aaagataggc aacttattac tcatcttatg
     1621 ctagccctca ataattgaat acgttgcact agaaccaatt atttctatga atgtttgctc
     1681 aaacataaaa atatagtatt taaagaattc tctattcaaa ggaaaataac tttctttcaa
     1741 tatccattcc gcataacact catcaagaag ttcaaaaaaa gattgaccac cattttcatg
     1801 aagcaaagct ctatctgcat cttccgaaat taaatccata aaagtatcta taaaatacga
     1861 ttcatcaaca accctaaaat gaataacatc ttcaataaaa atttttatgg ttttatccgt
     1921 atttctataa ccaactgcta taacttctac tttatcagag tttattgctg cattatcaaa
     1981 atagaaatag ccagttggga aatctaagct ttttaatttc ataatttatc gtgtatttcc
     2041 atatctgatt tttatttttc ggttattact aattgttatt tcaagtgttg gttctccacc
     2101 actactatca ttacgagcat taaccgcacg cccatcaggt aaattgcccg ttatcgttcc
     2161 attaggacgt gtttgtacat ttcgaacatt cagactatta aaatcattta gtgcgtcatt
     2221 gtaatccccc actttatcat actgaacagc gggacctttt gttttactcc tttgagtagt
     2281 attgctcaaa atgtctttga tttgtaaatc actgcgcgcc ttaccggctt ctatgcgagc
     2341 tgccacacta ctcgggctat aggggtcgct atcaatcgca ggattaccac cgctggtttt
     2401 ttgtccgcta gcctgtttcc caacctgctg tactgtatcg ccaatttcat ctaccgtacc
     2461 taacgcctta gtctgcatat ggcgaacagc aggtttttta tagccggtat ttttaaccca
     2521 atcccatttg gtcgggttaa gttctacttt tttacctctc caaaccgtac ctgcggcctc
     2581 tgctacggca agggcagttt gggctgttgc tgttatattc ggatgggcat cagcccattg
     2641 tctggcggaa ttgatgccgt cttttaccgc aaaagcactg tcctgtaata tgctcgcggc
     2701 agcaagttgt gcttccgggc ttaattttcc taaattatta ataccttgta gagtctgctg
     2761 cgcagagatg tagcaactgt atttttaccc cgtcgggtaa aaatacagtt gctacggctg
     2821 ctgggaaagc agttactatt ttcccagtgg ctcaaaatta attgtttcag cagatatttt
     2881 tataataatc cctacatcaa taataatttc actataaaat tggtatttat atagctctat
     2941 ttcgggtata aacaagggaa acaagctatc ttccttatcc tcataatcta cgaaattatt
     3001 tgtatctaat ttaaaattca aaatattact gcatttgata tatccgaaat aattaccttc
     3061 aattgtatct gtaaaagata atgtaagggt atttccatta tcggaaaaga ttatttcata
     3121 aaggcttaca ttgtgaaaaa tatttttttt catttgataa aatatccaga atgatgacga
     3181 ttatttggca atggattgcc ttcataattt tgtcgttgcg gatattttcc ctgtacacca
     3241 tcggttcgcg gcactctttg ggttcttata acctctccat ttggacgaat atctattttc
     3301 acgccatcag gacctttcca tgtttcatat tgagcctcat ctgaaccagt acgtctaaaa
     3361 ccttgttctt gcaatgcttg acgtaattca tctcgcgtag ttggtctgat agatttaaca
     3421 actggcttgt ttcctcctgc catctcaccg tccaacgtct gcatagggcg ggcagcaggt
     3481 gttttatagc cgttattttt aacccaatcc catttagtcg ggttaagttc tactttttta
     3541 cctctccaaa ccgtacctgc ggcctctgct acggcaaggg cagtttgggc agtagctgta
     3601 atgttcggat gggcatcagc ccattgtctg gcggaattga tgccgtcttt taccgcaaaa
     3661 gcactgtcct gtaataggct cgcggcagca agttgtgctt ccggacttaa ttttcctaaa
     3721 tcattaatac cttgtaaagt ttgctgcgca gagatgtagg tcggatattt gtatccgaca
     3781 aaaatatttg acgcatctat cgtttccgaa atgccgctgt tggaaaatgt cggattcgag
     3841 aatccgacct acggctattt cgtttaagtc ataaatagcc attcatgttc cttctgaatt
     3901 tgcttttgct atccttagaa ccaatcatat aaacattatc atcattaatt aatgttctaa
     3961 gtttatgaag cggtcaaatc tgtcaaacta ccagagatga agcatatcct cctggaagta
     4021 attctgttcg tgctgtttcc atatcttctt tgccatcatc agttacttct ctgtctaaat
     4081 aaaatcttac gataagtttt tgtctaattt catcaaattc tgccgtaatt aatcttatat
     4141 tgatatgaat acacggttca tacctgctat tagccagtca ggtgtagttg gatggggatt
     4201 catttttaat aatatcttca agcaagtgaa atgtaagaaa agcgtagata cttgaaggcc
     4261 ggctatctga ttggcaaaaa gaaatttcgg agataaagaa tttccgttta tatttttcat
     4321 aaattaaatc aacaccttgt tttacataaa aactaacttc ctcaggaagg atatttataa
     4381 cattctctcc ttttactttt atagttaatg gtttgatttt tatgttttca tgaatatctg
     4441 ataggattat ttctaaaaaa ttatttttat ttccagtcat tctggctatt ttatgtatat
     4501 ttccattgtg ttcgattatc attttatttt ttccaaggat ttccttctat attgatagtt
     4561 tatctaaaga tttcatttaa aatatcctga ctaatttctt gtttatataa ttctacaatt
     4621 ttctgtttct gtgtatttgt ataaaatttt tcgttggtat ctgcttgata ttttaattgt
     4681 ctcaatattt ccatgatttc atattctgat ttttcatcat attcttcaat catgtaatca
     4741 acaaattgtt ttagaatatc aaaggtttcg taactttcca aaattttaat atgtttatcg
     4801 gcaaaattgg acatatacca ttcgtcagtg attggattag tatttaaatc atctaaaaaa
     4861 acatttacat catctaaaaa catgactaat ccttaaactt aaaatctgtt ttctcaccgg
     4921 ttcttgagtt ttcaatgtaa tgtatttcaa ttttgtttac gttttgaaca cgcttaaccc
     4981 aaccgtcttt tgctaaccaa ccatttttag tatcggacat aggaggtatt ctcgcaggag
     5041 ttttgccctg tgggtttgcc ttaacttctt ccattgctaa ttgttccttt aaattattag
     5101 gtattgtacg accggttgat ttttcaacag attgtgtccc tatttttaat gatttgtttc
     5161 ccccagccat ttccccgtct acagtctgca tagggcgaac agcaggt
//
LOCUS       DAXHSJ010000141         5202 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000141 DAXHSJ010000000
VERSION     DAXHSJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.141"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(9..425)
                     /gene="dksA"
                     /locus_tag="ACFM8U_001716"
     CDS             complement(9..425)
                     /gene="dksA"
                     /locus_tag="ACFM8U_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC6334918.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(570..1379)
                     /gene="proC"
                     /locus_tag="ACFM8U_001717"
     CDS             complement(570..1379)
                     /gene="proC"
                     /locus_tag="ACFM8U_001717"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC6334919.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLGKELGVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVDTL
                     SRYLGGTRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDCKIADRIMKSVGLTIWLEDE
                     AQMHGITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAINEGVCACVRRSQEMERQYQ"
     gene            complement(1410..1811)
                     /locus_tag="ACFM8U_001718"
     CDS             complement(1410..1811)
                     /locus_tag="ACFM8U_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC6334920.1"
                     /translation="MRAIKHLAMALLLMFTVAGCQLAGWYKCSSLSGWCKPRKPAAID
                     FWDIVGESPPSLEDYEIPLSDGNRSVRVNEYESAQQSYFYRKIGKFEACGLDWRRRDG
                     KPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1816..2502)
                     /locus_tag="ACFM8U_001719"
     CDS             complement(1816..2502)
                     /locus_tag="ACFM8U_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC6334921.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGREPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVVLAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIACGATHVR
                     IGSAIFGKRG"
     gene            2622..3665
                     /gene="pilT"
                     /locus_tag="ACFM8U_001720"
     CDS             2622..3665
                     /gene="pilT"
                     /locus_tag="ACFM8U_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC6334922.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3828..5054
                     /locus_tag="ACFM8U_001721"
     CDS             3828..5054
                     /locus_tag="ACFM8U_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6334923.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
BASE COUNT         1311 a         1602 c         1174 g         1115 t
ORIGIN      
        1 tgccgccatc agtcggcaaa ctgttttttc atcctctctc ggcgttcttg ggactcaaca
       61 gataaagtgg ctgtcgggcg tgccagcagc cgcttcagac cgataggctc tcccgtatcg
      121 gcacagaatc cataatcccc ttcatcaata ttgcggatgg tcgcctgtat tttactgaga
      181 agttttcgtt cccgatcgcg ggtacggagt tccaatgcgt actcttcttc ctgtgtggca
      241 cggtcggcag gatcgggggc tgattcgtgt tcttggagat gccctgtcgt agcggaagca
      301 ttttcgatga gttcatcttg cattttaacc agcaattcac gaaaaaaagc caaatggtcg
      361 tcattcatat aatcgtcttc cggcccattc caattcaaaa tatcttgttc tgtcagcttt
      421 gccataatgt atgtcctctc agttcaccga aaaaaaagca ccacaaagtg gctttattcc
      481 tttgattttt ataaggctta ttgcctaagg cgtttttgaa aacgcgcatc ataacacgtt
      541 ttggattggt tttttttatt ttctctacat tattgatatt gccgttccat ttcctgcgaa
      601 cggcgcacac aggcacaaac gccttcattt atggcttcgg cgacacgatg ccgtctgaag
      661 gcttccacgg cttcgtgagt tgtcccgcct tttgacgtta catttttttg aagcttctcg
      721 aaatcttcac cagtctgctc ggcaagggca accgctcctt taaatgttgc cagactgagc
      781 gcgcgtgctt ctgccatatc aaacccttgt cggatggcgg cattttgcaa tgcgtccagc
      841 agataaaaca cataagccgg cccgctgccg ctgatgccgg taatgccgtg catttgcgct
      901 tcatcttcca accaaatggt caaaccgaca gatttcatga ttcgatcggc aatcttgcag
      961 tctgtttccg atacttccgc ttcggcatac ataccggata cgcccagccc gatttttccg
     1021 ggtgtattcg gcataacccg gacaatgcgg cgtgttcccc cgaggtaacg gctgagcgta
     1081 tcaaccgaca atccagctgc gacagaaagc accaatgcac cattggtgcg gatatttttg
     1141 cacgcggctt ccatatcctg cggcttgacg gcaaggatta aaacatcgtc ggaatgaagc
     1201 tccggcaggg ttgccgaagt ttcgaccccc aactcttttc ccaaacgttc gcgtttttcc
     1261 gcaccccgat tggcaatatg gatgcggtaa ccgccttgtt tgaccaatcc gcccgcaacg
     1321 gcagccgcca tattgccgcc gccgagaaaa taaacattca ttatttctac cctattcaaa
     1381 attacctgac taaattcatt cccaattttt taccatcgga cgcgctcgga cagaccgttg
     1441 cgccgcaacc cctgcttttt caaacaatca aaaccttcct gtttgaacgt ctcaatcaaa
     1501 ggtttgccgt cacgcctacg ccaatccaac ccgcaggctt caaacttccc tattttcctg
     1561 taaaagtaag attgttgtgc ggattcatat tcgtttaccc tgacggaacg attgccgtct
     1621 gaaagcggta tctcgtagtc ctctaaagac ggcggactct cgccgacaat atcccaaaaa
     1681 tcgatggcgg caggttttct cggcttacac cagccggaca gggacgaaca cttataccaa
     1741 cctgccaact gacagccggc taccgtaaac ataagcaata acgccattgc aagatgtttt
     1801 attgcgcgca ttccatcagc cccttttccc gaaaatcgcg ctgccgatac ggacatgtgt
     1861 tgcaccgcac gcaatggcgg caggcatatc gtccgacatc cccatagaca gcacgtccgc
     1921 cttaacgcca gccgcattga ggtcggcaag cagtttccgc atcgtttgaa attgcacctt
     1981 caactccgtt tcactgctgt tggctttggc aacacacatc aagccgcgta cgacgatatt
     2041 cggcagcttc gccacttcga ccgcaagcac gaccgcttct tcgggcgcga caccgtgttt
     2101 tgccgcctcg cccgcaatgt tcacctcgat acacacctgc aaaggcggca ttgaggaagg
     2161 acgttgcccg ctcagccgga cggcggtttt cagacggcat acggtatgca cccaatgcgc
     2221 gcgttcggcg acaaatttgg ttttgttgga ctgcacatcg ccgatgacgt gccacacgat
     2281 gtcggtcaaa tccgccaact cttccgtttt gccgtaccac tcctgaatat agttctcgcc
     2341 gaaatcacgc tgtccggcgg cgtaaacttc gcggatgccg tctgaaggga aagtcttact
     2401 gacggcaatc aggctgacgg aatgcggctc cctgcccgcc tgcagaacca attttccgat
     2461 acggtcggac acctcacaat aacgttcttg caacaccgtc atagattatc ccctaattaa
     2521 aaatgattaa acagttgaaa cccctccagt cagggcggta caatcaaggt tgttagaacc
     2581 attccaacca atcgaaacat tatactaaac agagccgcat tatgcagatt accgacttac
     2641 tcgccttcgg cgctaaaaac aaagcatccg accttcacct gagttcgggc atatccccta
     2701 tgattcgggt tcacggcgat atgcggcgca tcaaccttcc cgaaatgagc gcggaagagg
     2761 tcggtaatat ggtaacttcg gtgatgaacg accaccagcg gaaaatctac cagcaaaact
     2821 tggaagtcga cttctcgttt gaactgccca acgtcgcccg attccgcgtc aacgccttca
     2881 acatcggccg cggtcccgcc gccgtattcc gcaccattcc cagcaccgtc ttatcgctgg
     2941 aagaattgaa agccccgagc attttccaaa aaatcgcaga atcgccgcgc ggcatggttt
     3001 tggttaccgg ccctaccggt tcgggcaaat cgaccacgct tgccgcaatg atcaactaca
     3061 tcaacgaaac ccagccggca cacatcctga ccatcgaaga cccgatcgaa ttcgtccacc
     3121 aaagcaaaaa atccctgatt aaccagcgcg agctgcacca gcacaccctc agcttcgcca
     3181 acgcgctgcg ttccgcattg cgcgaagacc ccgacgttat ccttgttggc gagatgcgcg
     3241 accccgaaac catcggcttg gcactgaccg ccgccgaaac cggacacttg gttttcggca
     3301 cgctgcacac gaccggcgca gcaaaaaccg tcgaccgtat cgtggacgta ttcccagcgg
     3361 gagaaaaaga aatggtgcgc tctatgctgt ccgaatcgct gaccgccgtc atctcccaaa
     3421 acctgctgaa aacgcacgac ggcaacggcc gtgtcgcctc gcacgaaatc ctgattgcca
     3481 accccgccgt ccgcaacctc atccgcgaaa acaaaatcac gcagattaac tccgtcctgc
     3541 aaaccgggca ggcgagcggt atgcagacga tggaccaatc gctgcaatcg ctggtgcgcc
     3601 aagggctgat cgcaccggaa gtcgcacgca gacgcgcgca aaacagcgaa agtatgagtt
     3661 tctgacacac aaccgccttc cggccatacc ggcgggaaaa caaggcgcaa acacgcgggg
     3721 cgggacgcag catcccgccc ggctaccttt ccgaacagga agcgtccgcc ttcctgttga
     3781 aacctgccgc cgcaaactgc aaggcttaaa ccgaaaagaa gctaacgatg aataccgata
     3841 acctgcacga catcttggac gaaatggttc aagtgtattc tcaaaaaaaa caaagccgat
     3901 ccgaaacccc ggccgaaatc ggcgcacact tccacccgct gctcgaccgc ctgtgcgaaa
     3961 ccgcagaagc acaaaacgcg tccgacatcc ttatcagcaa aggattcccg ccctcgttga
     4021 aaatcaacag cgcattaacc ccgcagccgc aaaaggcgct gacgggcgag gaaaccgccg
     4081 ccatcgccgc atcgacgatg aacgccgaac aatcggaaat attccggcgc gacggcgaaa
     4141 tcaactactc cgtccagtcg cgcagcggca cgcgctaccg cgccaacgcc taccacagcc
     4201 aaggcagcgc aggtttggtt ttgcggcgca tcaaccacgt catcccgcaa atgcaggaat
     4261 tgggcctgcc cgaaaaactc aaagacctcg ccgtcgcacc gcgcgggctg ctgattatcg
     4321 tcgggcctac cggttcgggc aaatccacca cgatggcgac tatgctcgaa caccgcaaca
     4381 aaaccctgcc cagccatatc gttaccatcg aagacccgat tgagtttatc tacaaaccgc
     4441 gccgctgcat ctttacccag cgcgaaatcg gcgtcgacac cataaactgg cagacggcgg
     4501 tacaaaacgc tatgcgccag tcccccgacg tggtctgcat cggcgaagtc cgcagcaggg
     4561 aaagtatgga atacgcgatg cagctcgccc aaaccggcca cctgtgcatt tttacgctcc
     4621 acgccaacac cgcgccgcag tcgctcgaac gcatactcaa cttctacccc aaagaacagc
     4681 acaaccaaat actgatcgac atcgccctca atctgaccgg catcatctgc caacgcctcg
     4741 ccctcaaaca agacaaaacg ggcaggacgg cggttgtcga cttgctcatc aacacgcccg
     4801 ccatccaaga cttcatcctg aagggcgacc tgatgaacat cagtaaaatc atggaaaccg
     4861 ccaaaaccga cggaatgcag acgatggatc aaaacctttt cgaactgtac cgtcacggca
     4921 tcatcagtta cgaagaagcc ctgcgccagt ccgtttccgc caacaacctg cgattgcaca
     4981 tccaactgca caaagaaggc aaaacgcccg aactccttta cgacagggtc aacggtctca
     5041 acctcatttc ctgatccgca aaacccaatg ccgtctgaaa accgcatccc cgttttcaga
     5101 cggcatgatt ttatccgccc cattcatgtg ctacacttta tagtggatta aatttatagt
     5161 ggattaacaa aaatcaggac aaggcgacga agccgcagac ag
//
LOCUS       DAXHSJ010000142         5070 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000142 DAXHSJ010000000
VERSION     DAXHSJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.142"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(90..1445)
                     /locus_tag="ACFM8U_001722"
     CDS             complement(90..1445)
                     /locus_tag="ACFM8U_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC6334924.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAASKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASGSVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1460..1732)
                     /locus_tag="ACFM8U_001723"
     CDS             complement(1460..1732)
                     /locus_tag="ACFM8U_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC6334925.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENQINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1857..2594)
                     /locus_tag="ACFM8U_001724"
     CDS             complement(1857..2594)
                     /locus_tag="ACFM8U_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC6334926.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVVAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2875..3774)
                     /gene="prmB"
                     /locus_tag="ACFM8U_001725"
     CDS             complement(2875..3774)
                     /gene="prmB"
                     /locus_tag="ACFM8U_001725"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6334927.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAHIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFDGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPKLALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            3919..4512
                     /locus_tag="ACFM8U_001726"
     CDS             3919..4512
                     /locus_tag="ACFM8U_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334928.1"
                     /translation="MADGIGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIITKLDDLLRYAKYEGSYSDKKGHPDIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDGHKHYDHFILKDE"
     gene            4624..>5070
                     /locus_tag="ACFM8U_001727"
     CDS             4624..>5070
                     /locus_tag="ACFM8U_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC6334929.1"
                     /translation="MTKLYAEIAKREAQDDGTVKVLGYASIEAVDSDGEVIATAAMKA
                     AIPDYMKFDAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDNLNKSQITGLKLTEISLVERPANPDAVSTCFKADKSAE"
BASE COUNT         1388 a         1303 c         1127 g         1252 t
ORIGIN      
        1 attcttttct aaagacattt tacccgatgc ctataaacaa aaatgccgtc tgaagccttt
       61 cagacggcat ttccccatca aaaccgcaat cagtttttca tcgattgaac cggagccggg
      121 attctgccgc ctcggttaac aaacacttcg cacgaacctt ctttaaccgg catcacaggc
      181 gcgtagccca acaggccgcc gaactcgacg ctgtcgccga cggtttttcc ggttaccgga
      241 ataatgcgca cggcagtggt tttgctgttg atcatgccga tggcggcttc gtcggcaatg
      301 atgccggaaa tggtgtgcgc gggcgtgtcg ccgggaacgg caatcatatc caagccgacc
      361 gaacaaacgg cggtcatcgc ttcgagtttg tccagcgtca gcacaccggc ttcagcagcc
      421 gcaatcatgc cttcgtcttc agaaacgggg ataaacgcgc cgctcaaacc gccgaccgag
      481 ccggaagcca tcataccgcc ttttttcacg gcatcgttca gcaatgccaa agctgctgtt
      541 gtgccgtgcg taccgcagac gctcaagccc atttcttcaa gaatgcgtgc cactgagtcg
      601 ccgacggcgg gggtcggcgc cagcgacaag tcgagaatac caaacgggat attcagcatt
      661 tttgaggctt cgcggccgat gagttcgccc acgcgggtaa ttttgaaagc ggttttcttc
      721 actacttccg caacttcggt caatgtcgtt gcatctgaat tttccaacgc ggctttgacc
      781 acgcccggcc ccgatacgcc gacattaatc acagcatccg cttcgcctga gccgtgaaac
      841 gcgcccgcca taaacggatt gtcttccact gcattgcaaa acacgacgat tttcgcgcaa
      901 ccgaaacctt cgggcgtgat ttcagccgtg cgtttgatgg tttcgcctgc cagcttgacc
      961 gcatccatat tgataccggc acgcgtgctg ccgatattga tggagctgca cacgatatca
     1021 gtagttttca tcgcttcggg aatggaacgg attaacacct catctgaagg cgacatgcct
     1081 ttttgcacca gcgcggaaaa gccgccgata aaggacacgc cgatggcttt ggctgccttg
     1141 tccaaagtct gcgccacgct gacgtaagaa tcggctttgc tcgccgccgc gatttgggca
     1201 atcggcgtaa cggaaatgcg ctgattcaca atcggcacgc cgtatttggc agacagatat
     1261 tttgccgtcg ccaccaagtc tttgccgacc gtggtgattt tgttgtaaat gttttggttt
     1321 aacacgtcga tgtcggtgct gatgcagtcg tgcaaatcaa tgccgatggt aatggtgcgc
     1381 acgtcaaaat tctggtcggc caccattttg acggtttcta aaatctcgcc ggattggata
     1441 ctcatcacat tcctccaact caaatgcggt gcatcgcttg gaagatttct tcgttttgca
     1501 tacggatatc aagcgcgagt tttttgctct cttccgcaaa caaatccaaa acctcttgac
     1561 gcgatttgct gcattttgaa gtgtccacca agataatcat agtaaaaaaa tcgtccatca
     1621 gctgttggct gatgttgaga atattgattt ggttttccgc caaaattttg gaaacatcgt
     1681 acacgatgcc gacgcggtct ttaccgatga cggtgatgac tgaattgttc acaggcttac
     1741 tccttgcaga tatccgttaa agtccgaaat tataccaccg ttggattttg aagaaatatt
     1801 gtcaacaata tatacataca aaatgccggc tgaaactatt tcagacagca tcaaatttag
     1861 ggttcgatta aataaccatc cttatcccac tgggttttcc taaccaactt atcatcctga
     1921 taaacagcct cgctcttttt agaaccatct tcataccact ccaaaaccac cccgttgcgt
     1981 tgatggtggc ggatagacag ttccgagagt aatcggccgc tttcatccca agtcagaatt
     2041 ttggcaggct catcgttgac cataaccatt tccgtcttga tgctgccgtc ggcataccat
     2101 tgcttccata cgccgtttgc cttattttgc ttaaactgga tttcgctttc cttgccgccg
     2161 ttacggtaat agcggtaccc cgtaccttca ctcaaaccat ttttataagg cataacggca
     2221 gattttttac cgttcggata ccagttgacc cactccccgt ccggcttacc cttgctgaag
     2281 ccccccgcca tttttttctg accattaaaa tgccacaaaa tcaacatacc gttttgcagg
     2341 gtaggcacaa aagatttgat ttgcgttgaa gcaacgatat aaggttcaga atatttcttc
     2401 atcgacggat aataaaaatc ctgcgcgtgc gcaatacccg ccaccacact atattgcctg
     2461 atataagcgg cagaagacat cgtcgccgtc agctttccgt tctgattaaa ataaacagaa
     2521 taggtctgcg ccggcaaagc ggccgaaaaa cccaacagga cagttgaaaa tacaatccga
     2581 gataattttt tcattgcaat agcgatataa aaacaaggct gtgttttagt aatctgttga
     2641 tttcaattat ttgcaaggga aaagacaatt attttccggt taggaataaa cctattctat
     2701 tgaatatatt gaagccaagt acgcctatca acactatatt aaaacactgc caaaaacaat
     2761 taacttataa acaatatggt aaggatttct ctgccaagca tcaaacccga gacaacgtat
     2821 cgtaaaaatg ccgtctgaaa acaaatcgtc ttcagacggc atttcccctt caactcactc
     2881 ttcacccaat aactgctcgc gcgtcaagag gaaaacaaaa ccgtcgcccc cgctggtttc
     2941 caaccaagta aaaggcaact ccggatacgc tgcttccaat acatccctgt tatgcccgat
     3001 ttccaccagc aatacacctt tgggattcag aaactttgcc gcattcagaa gaatctgcct
     3061 ggtggcatcc aacccgtccg ccccgctgcc caatgccaat ttcggttcgt gcaaatactc
     3121 ttcaggcaat aactcaaccg attccgcatc cacataaggc ggattggaaa caatcaaatc
     3181 ataagtgcct tccaatccgt caaacaaatc cgtatgaata agccggatgc gttcttccaa
     3241 accataatct tcgacattaa tccctgccac ttccaaagca tccaagctca catcaaccgc
     3301 atcaatgtgc gcatcgggat agtgatgcgc catctgaatg gcaaggcaac cgcttccggt
     3361 gcaaagatcc aaagcattat gcaccagctc atcgtattct atccaaggac gaagtccgtt
     3421 acccaacaat tcataaataa aagaacgagg tatgattacg cgctcatcca catagaaatc
     3481 aaactctccc tgccatgcct ggtgtgtcaa ataagcggct ggaatgtgtt cgacagcacg
     3541 acgctcaata accgccagca cttcctcttt ttcagcttcc aagagttttg catcaagata
     3601 tggggcaagc atatccaaag gcaaattcaa agtatgcaga atcaaataag ccgcttcatc
     3661 atgcgcatta tctgttccat gaccaaaaaa gagccctgcc tcattaaaac ggctgactgc
     3721 aaaacgtaaa acatcgcgga tagtcgtcaa ttcttgtgct gcctgattaa acataatatg
     3781 aaccattctg cgtatagata cttttaatta taacagaaac aacaagcaaa ccttttcata
     3841 tcgccaaata accacccaat ctacccatac aactacataa atgcccgcgc gaaaaccatc
     3901 tcccgaacgg aaacggcgat ggcggacggt ataggcaatc tgattggctg ggaaaaaacg
     3961 gggcttgttg tcggtaagca gtggataacc gcaaaagacg acaaggtgtc cgatgtctgc
     4021 aatgccaacg gcgagatggg cgtaatcggg ctttacgagc ctttctcaca cggcgcattg
     4081 acgatacctg gtcatccgaa ctgccgatgc gaggttgttt ccgtatcggg tggcgaattg
     4141 ggggaatttg ccgaaaaaaa ggagcttcgt aaagcggcta tgcagtatgc gcgggataac
     4201 tttatcggca aaagctatgt caatgaaaac agcgggcatg aactgaaggt aacttggcaa
     4261 ggtgtgaaac acgctgcgtc aaaggcaaat caggcggaat tatccatcat aacaaaactt
     4321 gatgacttat tgcgctacgc aaaatatgag ggttcttatt cggataagaa aggtcatcct
     4381 gatattattg cagcacataa gtatcgtgcc gttgccaagg ttgggaatga gtctttaaat
     4441 atcggtgtga ttgtaaggga atttccagac ggccataaac attacgacca tttcatcttg
     4501 aaggatgagt aaagcccttt tgcagtgtcg ttctggagcg gatagcgtta aggcaagtac
     4561 acttccagcc ttacaaaagg gctttaaatt cagcatgcca tttatacagg caggagtaaa
     4621 cccatgacaa agttatacgc agaaatcgcc aagagggagg cgcaggacga cggcacggtc
     4681 aaggttttgg gttatgcctc aatcgaggcg gtcgattcgg acggtgaagt catcgcgacg
     4741 gcagctatga aggcggcgat tcccgattat atgaagtttg acgcggggcg cgagatgcac
     4801 ggctcaaacg ctgcgggaac ggcaattgaa atcaacgttg aagacgacgg cataaccttt
     4861 ttcggtgctc atatcatcga ccccgttgtc gtgtcgaaag tcaaaacagg cgtttacaaa
     4921 ggcttttcca tcggcggcag cgttaccgcc cgcgataatt tgaacaagtc gcaaatcacg
     4981 ggcttgaagc tgacggaaat cagccttgtc gagcgccctg ccaatcccga tgcggtgtct
     5041 acctgcttta aggcggacaa aagtgcggaa
//
LOCUS       DAXHSJ010000143         5000 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000143 DAXHSJ010000000
VERSION     DAXHSJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.143"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(212..2504)
                     /gene="znuD"
                     /locus_tag="ACFM8U_001728"
                     /gene_synonym="tdfJ"
                     /pseudo
     CDS             complement(212..2504)
                     /gene="znuD"
                     /locus_tag="ACFM8U_001728"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
     gene            2926..3516
                     /locus_tag="ACFM8U_001729"
     CDS             2926..3516
                     /locus_tag="ACFM8U_001729"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC6334930.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFMGLPNPVTCTRYHSLVIERGTLPECLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLVEFQNFKPQKI"
     gene            3580..4638
                     /gene="trpD"
                     /locus_tag="ACFM8U_001730"
     CDS             3580..4638
                     /gene="trpD"
                     /locus_tag="ACFM8U_001730"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC6334931.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRYVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4695..5000)
                     /locus_tag="ACFM8U_001731"
                     /pseudo
     CDS             complement(4695..5000)
                     /locus_tag="ACFM8U_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684347.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1139 a         1155 c         1419 g         1287 t
ORIGIN      
        1 cgcctgcgcg ggaatgatgg gtttctgctt ttgatttttc tgcttttgcg agaatgacgg
       61 cgtgaaagca agaatgatga aacaaagaaa atgggaatga tggcatagtg gtttgttctt
      121 tgtctttgcc atatttccta acaaattgat taaaaacaaa aaaggttttc agaatgccgt
      181 ctgaaaacct tttttgtttg cctgtccgat tttaaaactt cacgttcacg ccgccggtaa
      241 agctgcggcc catttgcggc gtatcagaga ggaagctgct gtgggcgtaa acggattggt
      301 tgagcaggtt gtcggctttg acgtaccaat tccactcgcc atagcgcgta ttgcggcggt
      361 agtttgcgcc gaggttgagc atatggtgtc cgggcgtgcg cgtttcgtag cgggcgagtt
      421 tgttttgggc gaacacgcgg tagtagtcca aattggcatc gatgcggtcg gtcagcgagg
      481 ctttcaggtg gacgccgagg cgcgcagccg gaacgcgagg ggcgttttgg tcgtcctgcg
      541 cgatgaaagg acggttgccg taggcatctt ccctgccggg tagggaaggc aggtttttca
      601 gacggcctcg tacatagtcg ccggaaacgc cgatgcggta gcgcggtgtc ggtttgaagt
      661 agatttcgcc ttctgcgccg tagaagtccg caccggattg gttgtagcgc acgagcttca
      721 tttcgctgtc gtcttcgatg gatttggggc cgcgtccgtc gtttaaggtt tgggcgtaaa
      781 tgtagttgcc gaagcggttg cggtagagtg ccagattgta ttgccagcgg tcgccttcgt
      841 agcccagcgc gagttcgata ttgttggaac gctctttgtt gaggtgtttg ttgccgactt
      901 caaaggtgtt ggtggcgacg tgtttgccgt gtgcgtacag ctcttgcgtt gacggcaggc
      961 gttcctgatg ggaggcgttc ctgatgggag gcggtcaggc tgagtttgtg ttgtggcgtg
     1021 aaataccagt tgcccgaaag tgcgaatgag cgggcggttt ggcggtgcgc gccgaggtcg
     1081 ggcaggggat ggttgtagta gttttcccga tcaatcaatg ctttatcgta gcggatggag
     1141 gctttttgtt tttccacgcg tacgccgcct tcaagcgtga agttgtccca gtttgcctgt
     1201 tctacaccga aaaagctgta atgttgcact ttattgtcaa gcagcatcgg ttgtttgacc
     1261 gcttcggatg tggcagataa agcactggat ttttgttgta aatattgcac gccccagctg
     1321 cctttcagac ggcctatggg ttggtggcgc aactcgatgc gggcgttttg cgtttggttg
     1381 ttaaaaaagt tttcgaccgc atcgcctgct ttttcgtcgt ggcggtagtc gttgcggttc
     1441 aagtatacgc gcagggcttc aaaaccgggg aacggttgct tccattcggc acggagttcg
     1501 tagcgtttgt tgcgcaggtc tatccacggt ctgccgctgt gggtgtgtgc gtgtgcatta
     1561 tcgtcgtcgt ggaagccgca gctcaagccc ggattgtcgt aatcgatgtc ttcttcggtc
     1621 aacaggtgcg gataaagctg taaatagcgt ttgttaatca agctcttttg ccagatgatg
     1681 tcggcgtggc aatcatcgta ttcgtggctg tgggcaggca gaccatattg gtcgcgacgg
     1741 tcgctgtacg ctacgccgat aaaacctttt tcgccaaccc aagacagccc gatgctgccc
     1801 gtttgcgaat cggcgtggct gtcgggcagg cgtttcagat tgcggtaacg cggtacggcg
     1861 taatcccccg atttgcggta cagcccttcc gtgtgcaata caaagttttt gcccaaaccg
     1921 atattgatgc cgccggacgt gagtttttcc agattgccgc tgctcaaacg caatccgagt
     1981 tcgcccgata cgccgttttc aggcattttt tcggggattt tgccatcggc aacatcgacc
     2041 agccccgcca cattgcccga gctgtacaag agcgtaaccg gcccgcgcag gatttcgacc
     2101 tgttgcgaca aggcggtatc taccataatg gcgtgatcgg gcgaaaaatc cgccatatcg
     2161 cctgtttcgc cgtgatggtt caacacttta atccgcctgc ctgtttgacc gcgaataacg
     2221 ggagcggacg cgccgccgcc gtattgcgag gcgtgaatgc ccggcacgcc gtccaaagca
     2281 tcgcccaagt tgacggcttt ttgtcgcaag gtgtcgccgc tgatgatttt gtcggaggcg
     2341 gtcgaagtgt gcagcagccc ggatgtggcg cgcggacggc ttttgccgac gacgctgacc
     2401 gtttccaagc ccactgattg ctcagttcca tgcgcttggg agaggagggg tgtgttgatt
     2461 aaaagaattg ataaaacaat gggtttgagt gtagtttgtg ccattttggc ttctcgtcgc
     2521 atttcaaaag tttgttatta tataacatta cattttttat atcataagat tttgagaaca
     2581 ctcagagggc ataggcaaaa gtttttcaaa tgacacggtt gcggcatcgg gcggtgttca
     2641 tttgtatccg ccgtcattcg ggggcgcggt tgatgttgac gcagattccg ctgtgtttgc
     2701 ccactatgtt cggctgcggg ataaccaaaa tttatgagtg cataaaaacg gtacgttccc
     2761 gaacggtcga aaaggtggca aaatggcgta cttgtcagaa cgcgaggctc tgcgccaggt
     2821 tcacgagggt gcatcgggca cgctaagata taagagggta tggatggggg tatcggaaat
     2881 gcagttaata acaaacaaat tataaatcaa taggttaatc acaaaatgct tttgtttatc
     2941 gacaattacg acagttttac ttacaacatc gtccagtatt tcgctgaatt ggggcaggaa
     3001 gtcgccgtgc gccgcaacga tgatattaca ttggaagaaa tcgaggcatt gaatccgcaa
     3061 tatctcgtta tcggccccgg tccgtgttcg cccaaagaag cggggatttc cgtggcggcg
     3121 atgcgccatt ttgccggccg gctgccgatt atgggcgtgt gtctcgggca tcagacgata
     3181 ggcgaggcgt tcggcggcag gatagtccgc gccaaaacgc tgatgcacgg caaggtgtcg
     3241 cccgtgtccc attcgggcaa gggtatgttt atggggttgc ccaatccggt tacctgtacg
     3301 cgttatcaca gcctcgttat tgaacgcggc acactgcccg aatgtttgga aatcacggca
     3361 tggacggaag acggcgagat tatgggcgtg cgccataagg aatatgccgt cgagggggta
     3421 caattccacc ctgaagctct gctgaccgaa cacggacatg atatgttaaa caatttttta
     3481 gttgaatttc aaaacttcaa accgcaaaaa atctgacgtg atgccgtctg aagcccttca
     3541 gacggcattt tcgtccgaat attgaacgga ggacaaaaaa tgattacacc gcaacaggct
     3601 atcgaacgat taatcagcaa taatgagttg ttttacgatg aaatgaccga cttgatgcgc
     3661 caaatgatga gcggaaaagt gccgcccgaa caaattgcgg cgattttgac aggattgcgt
     3721 atcaaggttg aaaccgtttc cgaaattacc gccgccgccg ccgtcatgcg cgagtttgcg
     3781 acaaaagtgc cgctggagaa tgcagagggg ctggtcgata tcgtcggtac gggcggggat
     3841 ggtgccaaaa ccttcaatat ttcgacgact tcgatgtttg ttgccgcagc ggcaggcgcg
     3901 aaggttgcca aacacggagg ccggtcggtc tcttcctcca gcggtgcggc tgacgtgatg
     3961 gagcaaatgg gcgcaaacct caacctgact cccgaacaga ttgcccaaag tatcagccag
     4021 accggtatag ggtttatgtt cgcacccaat caccacagcg ctatgcgcta tgtcgccccg
     4081 gtgcgccgtt cgctcggttt ccgaagtatt ttcaacatat tgggtccgtt aacgaatcct
     4141 gcgggcgcgc cgaaccagct tttgggcgtg ttccacaccg atttgtgcgg catcctttcc
     4201 agagtattgc aacagctcgg ctccaagcat gttttggttg tttgcgggga gggcggtttg
     4261 gatgaaatta cactgacggg caaaacacgc gttgccgagc tcaaagacgg aaaaatcagc
     4321 gaatacgaca tccgcccaga agatttcggt atcgaaaccc gccgcaattt ggatgaaatc
     4381 aaagttgcca atactcagga atctttgttg aaaatgaatg aggtgctgga aggaagagaa
     4441 ggggctgcgc gcgatatcgt attgctcaac accgccgccg ccctgtatgc cggaaatgtc
     4501 gctgcttcgc tttcagacgg catatctgcc gcacgggaag ccatcgattc aggcagggcc
     4561 aaatcgaaaa aagaggagtt tgtcggtttt caaccacaac aaagatgcca ttttcttgga
     4621 aagatggagc ttgggtgatg ccgccatgat tatggaactt ttgtggcaaa acataagcac
     4681 ttcacgaaga gaacttacca aactgttttt atataaaaac ttggggctgt actagataac
     4741 cagaccaaat tcccattaac taattgtctt aaaatctgaa tttgagattc tatttaaaat
     4801 gccattggca tttctttaaa tgcagcccca aatgctcttt gggaatgccg ttaaacttac
     4861 gtaaatggct aaattcacta atatcaagca catcataact acgaaaggta tccgtgtata
     4921 caatgccatc aggcttaact ttctttcgga taattggcaa caatgtcgct gattgcgcat
     4981 taggaacaac gacggtataa
//
LOCUS       DAXHSJ010000144         4934 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000144 DAXHSJ010000000
VERSION     DAXHSJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.144"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(807..1853)
                     /gene="recA"
                     /locus_tag="ACFM8U_001732"
     CDS             complement(807..1853)
                     /gene="recA"
                     /locus_tag="ACFM8U_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC6334932.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGVEMHIT
                     EGKLDETDGERPEE"
     gene            2107..2871
                     /gene="aroD"
                     /locus_tag="ACFM8U_001733"
     CDS             2107..2871
                     /gene="aroD"
                     /locus_tag="ACFM8U_001733"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC6334933.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVADAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPVVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2891..4906
                     /gene="rep"
                     /locus_tag="ACFM8U_001734"
     CDS             2891..4906
                     /gene="rep"
                     /locus_tag="ACFM8U_001734"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC6334934.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKSQTRGLTICTFHSLGMKILREDANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRTKAETSEAGELINNLLKEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGNPSD"
BASE COUNT         1224 a         1330 c         1326 g         1054 t
ORIGIN      
        1 gcgggaatct agaacgtaga atctgagaaa ccgttttact cgataaattt ccgtgccgag
       61 gggtctggat tcccgccttc gcgggaatga cggcatcagt ttgcaggatt cggcgtgaac
      121 ggtaaaaaca gtgagaatga taagaacgca aaaacggcaa gaatagcggg aatcggcagg
      181 ctgaagccca ccctaccatt atttacacat ccgtaccgct taaatgccgt ctgaaacttc
      241 gtcattcccg tgaaaacggg aatccagccc cgtgggagca gaaacttaca ccccgtcatt
      301 cccgcgaacg cgggaatcca gtaaccgaaa aaccacagga atctatcgga aaaacagaaa
      361 cccccgaccg tcattcccgc gaacgcggga atccagtaac cgaaaaacca cgggaatcta
      421 tcggaaaaaa cagaaaccct cgaccgtcat tcccgcgaac gcgggaatct agaacgtaga
      481 atctgagaaa ccgttttact cgataaattt ccatgccaac aggtctggat tcccgccttc
      541 gcgggaatga cggcatattt tttgcatttg atataaaggg tcgtttgaat tttgttcagc
      601 aagtgcaaag tgttgcacat aaaagggcgt aggcttcatc cgtaaagtcg gcgcaggata
      661 gaggcaaagc gggcgtaggt cgggctgtag caactgtatt tttcaccccg tcgggcaaaa
      721 ataccaaaac tcaaatcaag ccgttcggag gcagctcaaa aaacggtact tcgcaacaga
      781 agtaccgttt atcgggattt taagttttat tcttctgggc gttcgccgtc ggtttcgtcc
      841 aattttcctt cggtgatgtg catttctacg ccgttgaggg cgcgaatttt tgcgtcgatt
      901 tcgtcggcaa tttcgggatt ttccttcagc cagacgcgga cgttgtcttt gccctgaccg
      961 attttcgcgc cgttgtagct gtaccacgcg ccggatttgt tgatgatgtc gtttttcacg
     1021 ccgatgtcga tcaattcgcc ctcccaactg atgccttctc cgtagaggat gtcaaactct
     1081 gcctgacgga acgggggggc gactttgttt ttgatgactt tgacgcgggt ttcgttgccc
     1141 aatacttctt cgcctttttt gatggatccg gtgcggcgga tgtcgaggcg gacggaagaa
     1201 tagaatttca gcgcgttgcc gccggtggtg gtttcggggc tgccgaacat tacgccgatc
     1261 ttcatccgga tttggttgat gaacacaacc agcgtgttgg tttttttgat gtgtccggtc
     1321 agtttgcgca aagcctgact catcaggcgc gcctgcagtc cgacgtggct gtcgcccata
     1381 tcgccttcga tttcggcttt ggggacgagt gcggcgacgg aatcaacgac taccatatct
     1441 atgccgcccg aacggacaag tgtgtcgcag atttccaaag cctgttcgcc ggtatcgggc
     1501 tgggacaggt aaagctcttc gacttttacg ccgagtttgc gggcgtaaac gggatcaaag
     1561 gcgtgttcgg catcgacaaa ggcgcacacg ccgccgtttt tctggcattg ggcgacggct
     1621 tcgaggcaga gggtggtttt gccggaggat tcgggaccga agatttcgac gatgcgcccg
     1681 cgcggcagac cgccgactcc gagggcaagg tctaatccga gcgatccggt ggaaatgact
     1741 tcgaggtttt cttcctgctg gctgccgtcc attttcatga tggcgccttt gccgaaactt
     1801 ttttcgattt gcgccagtgc ggcggcaagg gctttgcttt tgtcgtctga catgggggtt
     1861 actccggaac aaatgcggta tgtgggatgc ggcgcaacac gggctgcggc gcgggatgtg
     1921 tatcgttttc ccgatgtgcg ggctatcggt aatgctgctt cacggggttg ccattatcgc
     1981 atatttcctt gcttgccgat atgcggcagg acgcggcggc ttgtgccgga atggaatctg
     2041 gatgccgtct aaaaggcggc cggctttgtt ataatggcgg ctgttttttc tgtgtgtgcc
     2101 tgttttatgt gttcctgcct tgttgtcaaa aataccgtta tcggaagcgg acgcaccaaa
     2161 atcgccgtgc cgcttgtcgc ccgcgatgct gccgaacttt ccgccgtact tgagcaaatc
     2221 aaaaatatgc ccttcgatat tgcggagttc cgcgccgact ttttggaatg cgcgggcagc
     2281 atcggcgaag tattgcgcca cacgcagacc gtccgcgacg cgctgcccga caaaccgctg
     2341 ctgtttacgt tcagacggca tggcgaaggc ggctcgttcc cgtgttcgga cgattattat
     2401 tttgaactgc tcgacgcgct gatcgaaagc cgcctgcccg acatcatcga catcgagctg
     2461 ttttccggcg aaaccgccgt ccggcgcgcc gtggcggatg ctcaaaaaaa cggcatcgct
     2521 gccctgctct gcaatcatga gtttcaccgc acgccgccgc aagaagaaat cgtatgccgt
     2581 ctgaaacaga tggaggactg cggcgcggac atctgcaaaa ttgcggtgat gccgcaaagc
     2641 gcggaagacg tgctgacttt actttccgcc acgctcgaag cgaaacggct tgccgccaaa
     2701 ccggttgtta cgatgtcgat ggggcagacg ggggcggtca gccgccttgc cggacaggtg
     2761 ttcggctcaa gcatcacgtt cggttcggga acgcaaaact ccgcgccggg gcaaatcggc
     2821 gtatccgccc tccgtgcgac actcgactgc ctcgaaaacg gagcagactg atttcagaca
     2881 gcatcaaaac atgatgaaac tcaatcccca acaactcgaa gccgtccgct acctcggcgg
     2941 cccactgctc gtccttgccg gtgcaggcag cggcaaaacc ggcgtgatta ctcaaaaaat
     3001 taagcatttg attgtcaatg tcggctatct gccgcatacc gtcgccgcga ttacctttac
     3061 caacaaagcc gctgcggaaa tgcaggagcg cgtcgccaaa atgctgccca aatcgcaaac
     3121 gcgcgggctg acaatttgca cgttccattc tttgggcatg aagattttgc gcgaagatgc
     3181 gaaccatatc ggttacaaaa aaaacttctc cattctcgat tctaccgaca gcgcgaaaat
     3241 catcggcgaa ctcttaggcg gtacgggcaa agaagccgta ttcaaggcgc agcaccagat
     3301 ttccttgtgg aaaaacgatt taaaaacgcc tgaagatgtc gttcagacgg cctctaatgc
     3361 gtgggaacaa caaaccgcac gcgtgtatgc gagctatcag gaaaccttac aaagctatca
     3421 ggcagtggac ttcgatgact taatccgcct gcctgccgtg ctgttgcagc aaaacagcga
     3481 agtgcgcaac aaatggcagc ggcggctgcg ttatctgctg gttgacgaat gccaagatac
     3541 gaatacctgc caatttacgt tgatgaaact tctgaccggc gcggaaggta tgtttaccgc
     3601 cgtcggcgac gacgaccagt ctatctacgc atggcgcggt gcgaacatgg aaaacctgcg
     3661 caaaatgcag gaaaactatc cacagatgaa ggtcatcaaa ctggagcaaa actaccgctc
     3721 caccgcgcgg attctcaaaa tcgccaacaa agtcatcgaa aacaacccca agctgtttac
     3781 caaaaaactt tggtcgcaat tgggcgaagg cgagcccgtc aaagtcgttg cctgccaaaa
     3841 cgagcagcac gaagccgact gggtcgtcag ccaaatcgtc aaacaaaaac tcatcggcgg
     3901 cgacaaaacc caatatgccg atttcgccgt gttataccgc ggcaagcatc aggcgaggat
     3961 tttcgaggaa gcattgcgcg gcgcgcgcat cccctaccgg ctctccggcg gacaaagctt
     4021 tttcgacaaa gccgaaatca aagacgtgtt gtcttatgtg cggctgcttg ccaaccccaa
     4081 cgacgatccc gcctttctgc gtgccgttac cacgcccaaa cgcggcatcg gcgatgtaac
     4141 gctgggcaag ctcaacactt acgcgcacga acacgaatgc agcctgtatg aagccgcgca
     4201 aaacgaagaa gcccttgcca cgctgaacaa taccaaccgc caacacctgc aagcctttat
     4261 ggatatgttc ggcagctacc gcaccaaagc cgaaaccagc gaagcgggcg aactcatcaa
     4321 caacctgctc aaagaaatcg attacgaaaa ccacctgctt gccagtgaag aaggcaaagc
     4381 cggcgaaatc aaatggcgca acgtcggcga tttggtatca tggtttgcgc gaaaaggcgg
     4441 ggaagacggc aaaaacatca tcgaactcgc ccaaaccgtc gccttgatga cgcttttgga
     4501 aggaaaagac gaagaagaaa ccgatgccgt ctcgctatcc acgctacacg ccgccaaagg
     4561 tttggagtat ccgtatgttt tccttgtcgg ttgcgaagaa ggtgttttgc cgcacaacga
     4621 cagcatcgaa gagggcaacg tcgaagaaga acgccgcctg atgtacgtcg gcatcacccg
     4681 cgccaaacgc caactcacgc tgacccactg cgtcaaacgc aaaaaacaag gcacatggca
     4741 gttccccgaa cccagccgat tcatagacga aatgccgcag gaagatttaa aaatattggg
     4801 gcgcaaaggc ggcgagccga ttgtcagcaa ggaagaaggc agacgcaacc ttgccgatat
     4861 aatcggaagg ctcgacagca tgaaaaaagg caatccgtcg gattaaacca agatagggaa
     4921 acgaagccgg agac
//
LOCUS       DAXHSJ010000145         4862 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000145 DAXHSJ010000000
VERSION     DAXHSJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.145"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..248)
                     /locus_tag="ACFM8U_001735"
     CDS             complement(<1..248)
                     /locus_tag="ACFM8U_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334935.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPAAEAPAA
                     EAPATEAPAAEAPATEAPAAEAPAAEAAATEAPAAEAPA"
     gene            721..1203
                     /locus_tag="ACFM8U_001736"
     CDS             721..1203
                     /locus_tag="ACFM8U_001736"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6334936.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            1290..2102
                     /gene="hpnC"
                     /locus_tag="ACFM8U_001737"
     CDS             1290..2102
                     /gene="hpnC"
                     /locus_tag="ACFM8U_001737"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC6334937.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(2174..2644)
                     /locus_tag="ACFM8U_001738"
     CDS             complement(2174..2644)
                     /locus_tag="ACFM8U_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC6334938.1"
                     /translation="MYDVNTHDVRRFFAHVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAENQARLNPHEIA"
     gene            2735..4576
                     /locus_tag="ACFM8U_001739"
     CDS             2735..4576
                     /locus_tag="ACFM8U_001739"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC6334939.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         1067 a         1081 c         1456 g         1258 t
ORIGIN      
        1 gcgggagctt cggcggcagg tgcttcagta gcggcagctt cagcagcggg agcttcagcg
       61 gcaggtgctt cagtagcggg agcttcagcg gcaggtgctt cggtagcggg agcttcagcg
      121 gcaggtgctt cagcagcggg agcttcggcg gcaggtgctt cggtagcggg agcttcagcg
      181 gctttttcac cgccgcaggc tgccaggacc aaagacaaca aagcagcggc aaacagggat
      241 tttttcattt tagtaacctt tagaaccaat ttaaattatt gaaacaaata ggtctttatc
      301 gaccatacta caaaccaggt tggaaattca tcgctgaatt tgcgtaatta tgcactatgg
      361 aataagggat tgcaatgtcg tacagatggg aaaaaggttt gaatgaaaag ttttctggcg
      421 ggcggattga taaatcctgt aaaacggttt tgctgttaaa aattcgatag ggataaatat
      481 tatagagatt aatataattt cagtatcttg tatgatttat taaggcggat tgggaaagtc
      541 ggcagacagg tcggaatggt tttaagaatg ttgtaaaaaa gttacaccgc aataaaataa
      601 caaaaattta cattgaaata tgtgctgtac agtttatgaa caggttaacg gtaatcggcc
      661 gacgcggcgg ttgtgttaga atgcgcctgt aatcaatctg ctgttcctga aaggacaaat
      721 atggctatcg tgaaaaattc cgtggtttcg ctgcattatg agatgtatga tgccaacaat
      781 cagcttttgg acaaaaccga agaaccgatt gcgtatctgc acggcggtta cgacggcatt
      841 ttccctttgg tggaagaggc gttgcacggt aaggatgccg gcgatacggt cgatgtggcg
      901 ctgtcgcccg acgatgcgtt cggcgagcag gatccggagt tggtccgtat tgaagatgcg
      961 ggcgtgttcc ctgttgaagt cgaagtcggc atgatgtttg aagccgacga tcctgaaacc
     1021 ggcgatgttg tcgtctatcg tgtaaccgat gttgccgacg gcaaggcggt ggtggacggc
     1081 aaccatcctt tggcaggcat gaaaatccgc tttaaggcta cggttgaaag tgtgcgcgat
     1141 gcatccgatg aggaaatcgc acacggtcat gtccacggtc cgcacggtca tcaccaccac
     1201 tgattgccgg cagcttggag gtatctgatg ccgtctgaaa agggtttgtt ccatttcaga
     1261 cggcattttg tttgatggat ggagaaggta tgtcggttgg gcattatgaa aattttcccg
     1321 tcggttcgct gattttgccg cgcaggttga ggaagccggt tcacgcggtg tacgcgtttg
     1381 cacggacggc ggacgatatg gcggacgagg gcagtatgcc gtctgaagcc aggttggcgg
     1441 ggttggaggg tttgcagcgc gagttggacg tgttggcatc gggcggccgg tcggcgcatc
     1501 ctttgattgc acgattggat gccgaggcgg ttgtgccgtt cggtttggat ttgcagccgt
     1561 tttatgattt gctttctgca ttttctcagg atgtggttaa aacaaggtat ggaaatttcg
     1621 gcgagctggc cgattattgt cggcgttccg ccaaccctgt cggacgcatt atgctggctt
     1681 tgtacgggaa aacggatgcg gtgtgcgtgg cgcaaagcga cggcatttgt acggctttgc
     1741 aactggtcaa tttctggcag gatgtggctg tggattggca aaagggcagg gtttatatcc
     1801 cgcaggacga tttgttgaaa ttcggtgttt ctgaggaaca gatcgcggca ggacgggcgg
     1861 atgcggcgtt tcagcggctg atggcgtatg agtgccgacg cgcattccgt atgctgaagg
     1921 cgggttcgcc tttggcaagg gaactgaagg ggcgtatcgg tttggaactc cgtatgattg
     1981 tgttgggggc gcagttgatt ttgcagaaac tggacgcgtg ccgatacgat gtgtttgcac
     2041 aacgccccgt tttggataag aaggattggt tgattatgct gaaaaaggcg ttgtggaaat
     2101 gattggggga aatgcatgcc gtctgaaagg ggttgttccg cttcagatgg cattttgcag
     2161 gcggttggta tggtcaggcg atttcatgcg ggttcaacct ggcttgattt tctgcaccca
     2221 agccgatgag tttgcgcagt cgggtcatgt agaaattgac atccaaaccg gtgccgtagc
     2281 gttgcgccgt ccacagtgtt tccgccagtg cctccatcat ttcgtgttcg gcttccagcc
     2341 agccgcgttt ggcgcacagg gtgtcgtgga tggcgcgtat gccgtgcggc tggtctatgc
     2401 ccgcctgttc ttggacggac agatgcagcg acatatgcag gaaggggttg ctttcgccgt
     2461 tttcgggcag ccagtcggtg tccagatggt cttcgatgcg ttcgagatag cggtggtatt
     2521 cgggatgggc ttcgacaatg cggagggctt tctgttccag tgcgctcagt tgcagcggat
     2581 tgagccgctg ctgccacacg tgggcgaaaa agcggcggac atcgtgggta ttgacatcgt
     2641 acatggcggg ttcgataaga tgtttatgga aataggttcg gataatggcg ggttttacat
     2701 aaattaaatc ccaccgtcaa ccttaccatt tataatgcag acaaattatt tgtctgaaac
     2761 ggattcgatt atgaaaaaac tgatttgcct gttcgccgta tttttgatgt tgtgcggacg
     2821 agctttcgcg ctggatgcga acgatctgct gccgccggaa aaggcattcg tgccggagct
     2881 tgccgttgcc gacgacggtg tgaacgtccg tttcaggatt gccgacggat actatatgta
     2941 tcaggcgaaa atcgtcggca agaccgatcc ggcggatttg ttgggacagc cttctttcag
     3001 caagggcgaa gagaaggaag acgagttttt cggcaggcag acggtttacc atcacgaagc
     3061 gcaggttgcc tttccttatg caaaggctgt cggcgaaccg tataaattgg ttttgaccta
     3121 tcagggctgt gccgaagccg gcgtgtgcta tccgcccgtg gataccgagt ttgatatttc
     3181 cggcaacggc acttaccatc cgcaaaccga cgaaccggca tccgccaaag atcgcttttt
     3241 gcagccttcc tctcaaaacg gcagcggggc gctgccgccc ccgaaggggg atgagggcgg
     3301 cgacagccgt ttcaagctgt cttgggatac gctcaacgcc aatcttttgg cgttttttct
     3361 cgccggtttg ggtctgagtt ttaccgcctg tatgtatccc ctgttgccga ttgtttccag
     3421 tattgtggtc ggcgacaaaa aggcgggcaa ggcgcgggcg tttgtgctgt ccgtcgttta
     3481 tgttcagggt ttggctctga cttatacgct ggtcggcatt gttgccggac tgacgggcgc
     3541 gctgctgacc gtatggttgc agcaggcttg ggtcgtgttg gcggcatcgg ctttgatggt
     3601 cgtcttggca ctgtctatgt tcgggctgtt caacatccag cttcccaacg ccgtgcagtc
     3661 gtattttcag aatcaaagca gcaggctttc aggcggtaaa atcgtttccg tctttattat
     3721 gggcatattg tccgcgctga ttgtcgggcc gtgcgtcgcc ccgccgctgg catttgcttt
     3781 gggctacatc ggtcagacgg gcgatgcggt tttaggcggt ttggcacttt acactttggc
     3841 gttgggcacc ggcgttccgc tgattgccat cggcacgttc ggcgggcata tcctgcctaa
     3901 ggcaggcgat tggatgaatg ccgtcaaata cgctttcggc tttatcctgc ttgccgtcgc
     3961 cgtttacctc gccacgccgc acttgcccta ttatctcgtc gtcgcgctgt acacgctgct
     4021 gatgctggtt cctgcctgta tgctgctggt caacggacgc aggcagaaac gccgtccgaa
     4081 agctgtggca ttcgcattgg gcggcatatt gctgataggc ggcgcgtggt tcggctggca
     4141 gggcgcaaac ggcaaaacga ccgcgctgca ccatttcctg accctcaatc cgccggccga
     4201 agcaggcaaa tcttcggaac acggaaaaat gtttgccgat actgccgcgc tgaaggcagc
     4261 gatggatacg gcgttgaaag aacatcccga caaacccgtc gttttggatt tttatgccga
     4321 ctggtgcatt tcctgcaaag aaatggcggc ttacacgctt aatcagccgg aagtgcatca
     4381 ggcagtcgat atggaacgct ttttccaaat cgacgtaacc gccaacacgc ccgaacatca
     4441 ggcgttgttg aaagaatacg gtctgttcgg gccgccgggc gtgtttgtcg tccgctccga
     4501 cggcagccgc agcgagccgc tgctgggttt tgtcaaagca gacaagttta tcgagtggta
     4561 tgaacaaaac cgctgattcc gatgtttcaa atgccgtctg aagttctcag acggcatttt
     4621 tgtcagaaag gaaaacggca atatccgcta tacacacgga agcagtccaa agatgtgaaa
     4681 accgtgtgta taagttagtt tgcgtattga aattaaaaga taaaaatacg atgattaaaa
     4741 aataggcata agaaaagata aacacagccg gtgtttgccg gtgtgtgtat cttttgtgga
     4801 aaaacatctt aatgtcatgt attttatgga aaataataac atgattaaaa aatagtcaat
     4861 ca
//
LOCUS       DAXHSJ010000146         4850 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000146 DAXHSJ010000000
VERSION     DAXHSJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.146"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(257..985)
                     /gene="rmpM"
                     /locus_tag="ACFM8U_001740"
     CDS             complement(257..985)
                     /gene="rmpM"
                     /locus_tag="ACFM8U_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC6334940.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1349..2299
                     /locus_tag="ACFM8U_001741"
     CDS             1349..2299
                     /locus_tag="ACFM8U_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC6334941.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2412..3146)
                     /gene="fnr"
                     /locus_tag="ACFM8U_001742"
     CDS             complement(2412..3146)
                     /gene="fnr"
                     /locus_tag="ACFM8U_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC6334942.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3351..4772
                     /gene="hemN"
                     /locus_tag="ACFM8U_001743"
     CDS             3351..4772
                     /gene="hemN"
                     /locus_tag="ACFM8U_001743"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC6334943.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTVAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1234 a         1328 c         1173 g         1115 t
ORIGIN      
        1 cggcaatggg acacaaacct gaatctgaat ctgaatctga atctgaatct gaatctgaat
       61 ccgaatccga atccgaatcc gaacctgcac cccgattctg acgatagccg tttcagaaaa
      121 ccacccaaaa aaacaaaccc cgcaaaagcg ggggtttgtt tcacatcatc gatgccgacc
      181 gacgtttcac gaatattgca acagtacccg gtacaaaatc cgctaacctc atgcatcggc
      241 aagatattgc ctagccttag tgttggtgat gattgtgtgc cggcacaacc tgacgggtta
      301 cgatgctgcg gattttcaca tccacacggc ggtcaggttc gatacatgca atcagagcct
      361 cacgtttttt ggctttagag actttcgcac ccagtttggc aacttcggct tcacaaactt
      421 gagtcatttg cgcttgagat tcgcccaagc cgacagcaga aattctagaa acaggtacgc
      481 cgttgctgac caggttgttt gccactacgt atgcgcggcg ttcggacagg gcctgattgt
      541 atttgtcaga acccataaag tcggtatggc cttcgacgcg gacagattgg acattggttc
      601 gactcaggcg ttgcgccaat actttcaggt tgtcttgagc ttcggcgcgc aatgaatcct
      661 tatcgaaacc gaacagggtt ttggcagaca gggaaatggt ttcatcaaca tattgcggag
      721 cctgctccac aacgacgaca ggcgcgggtg cgggttcggg ttctggctcg ggttcggggg
      781 cagcaaccgc atcgccgcat tctacgcgac cttggcttgc tttatcaaag taggcgtttt
      841 tccagcattc gccatagttg ttgcgtacga tttcgttcga ctggccgctt acggtgtaac
      901 cctgaacgga cgcctcgccc gcaacagcag tgccggaagc gagcaatgca acgaataatg
      961 cgcttaattt cagctgtttg gtcattttat tccctcatta aatttgtaca gcagatacag
     1021 aagcaactgc tgaattgcca acatacaggc cgctgtagaa acatggcggc aaatcggata
     1081 tttcagtctc cactataaag aagcaggggg aggactgtca atactcaacg cctgaaaaca
     1141 accgtaaaac agcctgtttc ctgatagtct atcatgccga aaaatcaatt ccgtgtcatt
     1201 actttagggg gattttttcc atatcgcacg attgccgttg cacaaaaaca acaaacacaa
     1261 cagcaaagcc ccataccgta ccggcaagaa aatatgataa attataaaca atgttcagcc
     1321 acccgacaca gacccacacc gacccgccat gaaattacaa caattgaaat acgccttaga
     1381 agtttaccgg cacaacctga acgtttccga agcggccgaa gccttgttta cttcgcaacc
     1441 cggcatctcc aaacaaatca aattgctgga agaagaaatc ggcattcaga tttttatccg
     1501 cagcggcaag cgcgtggttt cggtctcgca gccgggcaag gtggttttgg atattgcaga
     1561 acgtattttg cgcgatgttc agaacattaa aaatatcggc agcgagttta ccggacagga
     1621 cagcggttcg ctgacggttg ccacgacgca tactcaggca cgctatgccc tacccttgat
     1681 tgttgccgat tttgtgaaac gctatccgaa agtcaacctg accatcaaac aggggagccc
     1741 tgccgccatc gcccaaatgg ttacttcggg agagtctgat ttggcaattg ttacggaacg
     1801 tatagacgac caccccgaac tgggaagact ttcctgctac gactggacac acgcggttat
     1861 cgtaccgaac gaccacccct tgctcgaatg cagaaacccc ctccgtattg aagatttggc
     1921 gaggtttccg ctgattactt atgaatttgc attcaatgcg ggcagcagca tcgcgcgggc
     1981 attttccaaa gcccgtttgg aacagccgga tgtcgcattg gcggcggcgg atacggacgt
     2041 attgaagact tatgtgcgct tgggtttggg cgtgggactg atggcgaaaa tggcgtacaa
     2101 cccggatacg gacggcgatt tgcagcttgt ggatgcggca cacctgttcg agccgtcgcc
     2161 gacgtggatt gctttgcgca gcgatactta tttgcgcgga tatgcctacg actttatcca
     2221 agcgtttgcg ccgcacctga cacgcgagaa ggtggatagg attctgtaca cgcccatcag
     2281 cgaggatttt tcgatttagg cggctgccgg ttttcagacg gcactttgcg gcagatacaa
     2341 caaacaggac agatgttttc gtctgccctg tgtttattga gaatgccgtc tgaaatgttc
     2401 gtacgggtta atcaaatggc gtgcgagcag ccggacacca tttttttcaa cacctgcaga
     2461 ttgaggattt tgatgtgctt atgctcgacg gaaatcaatc cttcctgatg aaatttagat
     2521 aatgtgcggc tgacggtttc aagtttcagc ccgagataac tgccgatttc ttcgcgggac
     2581 attcttaaga tgaagtcgtt ggcagcaaaa cctcgggaat aaaggcgttg ggaaaggttc
     2641 agcaggaagg cggcaatccg ctcttcggcg cgcatattgc ccaacagcag cataacacct
     2701 tggtcgcgca cgatttcacg gctcatcatg cggaagaagt gcgtacgcag gctggggatg
     2761 ttttgcccca gttcttcgat gtgggtaaac ggcagttcgc acacttcgct gtcttccaag
     2821 gcgaccgcgt cgcaactgtg cacatgggaa cagatgccgt ccatgccgat gagttcgccc
     2881 gacataaaga aacccgttac ctgatcgcgg ccgtcctgac tggcgacggt tgttttgaag
     2941 aagcccgaac ggatggcaaa gagcgaggta aaggcttcgc cgacacagaa caggtattcg
     3001 ccctttttca ggcggcggct ttgacggatg acggcatcga gttggctgag ctcgttgggc
     3061 agcagcccga caggcaggca gagttcccgc aaagaacagg aagaacacag cgttttcatc
     3121 tgatgtgtag tattatgcga agccataccg tacctttttg tgcgctttgc cccatcatga
     3181 ttatagtgga ttaaatttaa accagtacgg cgttgcctcg ccttgtcctt atttaaattt
     3241 aatccactat atgtgcttat tgacacatat caagacaggt ttatcatact gtggcattct
     3301 accaaactat ccaaaatttg actaacatca taaaaccgca cacacacacc atgaaaatca
     3361 ttcagataca gaacaatcac aatgtaaacg atgaccgccc cgagtttgac cgcgcgctga
     3421 ttgccagcct gcccgccagc ggcccgcgct acacttccta ccctaccgcc gaccgtttcc
     3481 atgacggttt ccgcgaaggc gaatatatca aagctttaca tttgcgcggt atgggcgcgt
     3541 taaacaaacc gctttccctt tacattcaca ttccgttctg caacaccatc tgctactact
     3601 gcggctgcaa caaaatcatc accaaagaca aaagccgcgc cgatgcctac atcgaatatc
     3661 ttgaaaaaga aatggaactg ctcgctccac atctgaacgg acggcaccag cttgcccaac
     3721 tgcacttcgg cggcggcacg ccgacctttt tgagcgacga acagatcgaa cgtgtcttcc
     3781 gcatgatacg caaacatttc gagttaatcc ccaccggcga atactccatc gaaatcgacc
     3841 cgcgcaaagt cagccgcgac accgtcctca tgctcggcag gctcggcttc aaccgcatga
     3901 gcatcggcat tcaggatttc gaccccaaag tgcaggcggc ggtcaaccgc atccaaagtt
     3961 acgaagaaac caaagaagtc atcgatgcgg cgcgcgaagc ggggttcaaa tccgtcagcg
     4021 tcgatttgat ttacggcctg ccgcaccaga cttcggaaag catcaaaacc accatcgata
     4081 ccgttttgtc gctcgatccc gaccgcctcg ccctttatca ctacgcccac ctgccgcacg
     4141 tgttcaaacc gcaacgccgc atcgataccg tcgccgttcc cgacagcgaa gagaagctcg
     4201 atatgctgca atactgcgtc caaaccctga cagagcgcgg ctacgtcttc atcggcatgg
     4261 atcatttcgc caaacctgac gacgaactct ccatcgccct caaagaaggc ttcctccagc
     4321 gcaacttcca aggctattcg acctacgcgg attgcgattt ggtcgccatc ggcgtgtcgt
     4381 ccatcggcaa aatcggcagc acctattccc aaaacgaacg cgacatcgat gcctactatg
     4441 ccgccatcga cgaaggcaga ctgcccatca tgcgcggtta tcagctcaat caggacgaca
     4501 tcctgcgccg caacatcatt caggatttga tgtgccgttt cgcgctcgac tatcggattt
     4561 acgaaagtat gttcggcatc ccgttcgacc gctacttcaa agacgaactg gcggatttgg
     4621 aaaaactcgc cggcttggga ttggtgcgcc tgaacagcca cgggctgacc gttaccccaa
     4681 aaggacgctt cctcatccgc aacatcgcca tggtcttcga ttaccacctg cgccataaag
     4741 aaaccaaggc gaaatattcg caaacggtgt gattgcgtct aacgtacaaa tgccgtcggc
     4801 aggatatcat aacgttccgc acctttgtgt ccgaccgttc cgaaaccaag
//
LOCUS       DAXHSJ010000147         4781 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000147 DAXHSJ010000000
VERSION     DAXHSJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.147"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            207..1049
                     /locus_tag="ACFM8U_001744"
     CDS             207..1049
                     /locus_tag="ACFM8U_001744"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC6334944.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFQPVGTTDSERAFCHILNRLRTRFVARPDDDTLFGAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDETWAQLAVDELVMFREGKIILNDRPRHPVYMSVEEGLETARAA
                     GVAV"
     gene            1079..1543
                     /locus_tag="ACFM8U_001745"
     CDS             1079..1543
                     /locus_tag="ACFM8U_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC6334945.1"
                     /translation="MKCPFCAHPDTRVADSRLMDERNAVRRRRYCPNCGKRFGTLETA
                     ELKMPAVIGTDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETARLTELRLYASGLRD
                     ISAAELVEIVLEELFGQSTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            1575..2684
                     /gene="ribD"
                     /locus_tag="ACFM8U_001746"
     CDS             1575..2684
                     /gene="ribD"
                     /locus_tag="ACFM8U_001746"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC6334946.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAQDSQIVGQG
                     FHVKAGEPHAEVHALHQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLESAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLHPYRKHAHVRILMPSETAGGK
                     IDLNSLMRLLADEGIGELIVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSTEILGHDIKTVFRKNDNAF"
     gene            2879..4300
                     /locus_tag="ACFM8U_001747"
     CDS             2879..4300
                     /locus_tag="ACFM8U_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC6334947.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEENAPPARLSATAESASALLAAAVCLTGIFSPLASLLLPENYTAVRFIVVSCMLPP
                     LFCTLAEISGIGLNVVRKTRPIALATLGALAANLLLLGLSVPSGGARGAAVACAASFW
                     LFFAFKTESSCRLWQPLKRLPLYLHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            4297..>4781
                     /locus_tag="ACFM8U_001748"
     CDS             4297..>4781
                     /locus_tag="ACFM8U_001748"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6334948.1"
                     /translation="MKIILTTSMAGLGGTETATVRLGRLLKRRGHDVILASSDGPFVG
                     EAQASGIRWQPVDFYRGGLAGYLKSTFTYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPAGRIAYAY
                     NA"
BASE COUNT          976 a         1609 c         1222 g          974 t
ORIGIN      
        1 ctcaaaagtt acggaacgaa aaacaaccaa aaccggacaa gtcggattcc cgcctgcgcg
       61 ggaatgacgg aatcttaagt ttccgtcttt gttttctgtt ttctgttttc gagggaataa
      121 tggggaacaa gccgtatttc agacggcatt ttcagttcgg ggtataatcc gaatacttgc
      181 gaccatctga atcatttgga caaactatgt gtcaactgct gggcatgaac tgcaatacgc
      241 cgaccgatat tatgttttcc tttgaaggct tccgccggcg cggcggcatt accgaccatc
      301 atgccgacgg tttcggcatc ggctttttcg aaggcaaagg cgtgcgcctg ttccatgacg
      361 acaaacccag tgccaattcg cccgtcgccg acctcgtgcg cgcctaccag attaaatcgg
      421 aaaacgtcgt cgcccatatc cgcaaagcat cgcaaggaca aacctcgctg gcaaacaccc
      481 atccctttat gcgcgaaatg tggggcggct actggctgtt tgcccacaac gggcatttgg
      541 tcgacttctt tcccgaacag ggcgaatttt tccaacccgt cggcacaacc gattccgaac
      601 gcgcgttctg ccacatcctc aaccgcctgc gcacacgctt tgtcgcccgt cccgacgacg
      661 acacgctgtt tggcgcgatt gcggggctga cgcacgaaat ccgcaaattc gggctgttta
      721 actttatgct ttcagacggc attgccctgt ttgcccacgc cagcacactg ctgcactaca
      781 tcgtccgcca agccccgttc ggcaaggcgc gcctgctcga cgacgacgta atggtcgatt
      841 ttgccgaagt aaccacgccc tccgaccgcg tcgccgttat cgccaccctg ccactgacgc
      901 gcgatgaaac gtgggcgcaa cttgccgttg acgaactggt gatgttccgc gaaggcaaaa
      961 ttattttgaa cgaccgaccc cggcaccccg tttatatgag tgtcgaagaa ggtttggaaa
     1021 ccgcgcgcgc cgccggcgtc gccgtctgaa cttcagacga cataggagga cgaacccgat
     1081 gaaatgcccg ttttgcgccc accccgacac ccgcgttgcc gattcgcgcc tgatggacga
     1141 acgcaacgcc gtccgccgcc gccgatactg ccccaactgc ggcaaacgct tcggcacact
     1201 cgaaaccgcc gaactcaaaa tgcccgccgt catcggcacg gacaaaaaac gttcgccctt
     1261 caatgcacaa cgcctccgca acgacctgac cgccgccgcc cgaaaatccg ccctgacacc
     1321 cgaacagatc gacgaaaccg cccgcctcac ggaactcagg ctgtatgctt cgggtttgcg
     1381 cgacatatcc gctgccgaac ttgtcgaaat cgtactggaa gagctgttcg ggcaaagcac
     1441 ggaagccgcc gtccgtttcg ccgccctgca caaacgcttc gacaatccgg cagactttgc
     1501 ctcgtggctg gcgcaagccg tcaaaacagg cggcaaagcc tgattccccc aacccatact
     1561 gatacggtat ccccatgttt tcggacacag atatatccat gatggaaaac gccctccgac
     1621 ttgccgcttt ggggcgtttt tccacttcgc ccaatccgcg cgtcggctgc gttatcgcac
     1681 aagacagcca aattgtcggg cagggcttcc acgtcaaagc gggcgaaccg cacgccgaag
     1741 tccacgccct gcatcaggcg ggggaaatgg caaaaggcgc gaccgccttt gttaccctcg
     1801 aaccgtgcag ccattacggg cgcacaccgc cctgcgccga agctctgctc cgttccggcg
     1861 taacacgcgt cgttgccgcc atgcgcgacc ccaacccgct ggttgccggc aaagggcttg
     1921 ccctgctcga atcggcaggc atcaaaacag aatgcggact gcttgaaaac aaagcacgcg
     1981 aactcaaccg gggattttta tcgcgcatcg aacgccgccg tcccttcgtc cgcctcaaat
     2041 gcgccgtttc gctggacggc aaaaccgccc tttcagacgg cagcagcttt tggattaccg
     2101 gcgaagacgc gcgtgccgac gtacaggttt tgcgtgccga aagctgcgcg gtgctgaccg
     2161 gcatcggcac ggtgttggcg gacaatcccc ggctcaacgt ccgcgctttt ccaactttgc
     2221 gccaacccgc acgcatcgtt ttagacagcc gcctgcgcct gcccttggac agccaccttc
     2281 tgaccgacgg acaatctcca acctacatcg ccaccttgga acgcgatgaa gacaaactcc
     2341 acccctatcg caaacacgcc cacgtccgca tcctgatgcc gtctgaaacg gcaggcggca
     2401 aaatcgattt gaacagcctg atgcgcctgc ttgccgacga aggcataggg gaactcatag
     2461 tcgaagcagg ctccgaactc acatctgcat ttttggcaga agatttggca gacgaaatcg
     2521 tgctgtaccg ttcgcccaaa atcctcggca gcggcaaaga cctgctttcc ctgcccgaaa
     2581 accgcgccgc cctttccgca ccgcccttgt ggacacccgt ttccaccgaa atcctcggac
     2641 acgacatcaa aaccgtgttt cggaaaaacg ataacgcctt ttaaagggtt tgcgccgttt
     2701 cactatataa taacgccgat aaaaaacggc accattcaga ccgccgccca cccgaaaaag
     2761 cgcaacccgg actgccgcac cccgccggca gccgcgacgg tctgaaagcc gtcaaattcc
     2821 gatcaagaaa ggcttcagac ggcataggca gcatcccgcc tccgtccgga catcaaaaat
     2881 ggacacaaaa gaaatcctcg gctacgcggc aggctcgatc ggcagcgcgg ttttagccgt
     2941 catcatcctg ccgctgctgt cgtggtattt ccccgccgac gacatcggac gcatcgtgct
     3001 gatgcagacg gcggcggggc tgacggtgtc ggtgttgtgc ctcgggctgg atcaggcata
     3061 cgtccgcgaa tactatgccg ccgccgacaa agacaccttg ttcaaaaccc tgttcctgcc
     3121 gccgctgctg tctgccgccg cgatagccgc cctgctgctt tcccgcccgt ccctgccgtc
     3181 tgaaatcctg ttttcgctcg acgatgccgc cgccggcatc gggctggtgc tgtttgaact
     3241 gagcttcctg cccatccgct ttctcttact ggttttgcgt atggaagggc gcgcccttgc
     3301 cttttcgtcc gcgcaactcg tgcccaagct cgccatcctg ctgctgctgc cgctgacggt
     3361 cgggctgctg cactttccgg cgaacaccgc cgtcctgacc gccgtttacg cgctggcaaa
     3421 ccttgccgcc gccgcctttt tgctgtttca aaaccgatgc cgtctgaagg ccgtccggcg
     3481 cgcaccgttt tcatccgccg tcctgcatcg cggcctgcgc tacggcatac cgatcgcact
     3541 aagcagcatc gcctattggg ggctggcatc cgccgaccgt ttgttcctga aaaaatatgc
     3601 cggcctagaa cagctcggcg tttattcgat gggtatttcg ttcggcgggg cggcattatt
     3661 gttccaaagc atcttttcaa cggtctggac accgtatatt ttccgcgcaa tcgaagaaaa
     3721 cgccccgccc gcccgcctct cggcaacggc agaatccgcc tccgccctgc ttgccgccgc
     3781 cgtctgcctg accggcattt tctcgcccct cgcctccctc ctgctgccgg aaaactacac
     3841 cgccgtcagg tttatcgtcg tatcgtgtat gctgccgccg ctgttttgca cgctggcaga
     3901 aatcagcggc atcggtttga acgtcgtccg caaaacacgc ccgatcgcgc tcgccacctt
     3961 gggcgcgctg gcggcaaacc tgctgctgct ggggctttcc gtaccatccg gcggcgcgcg
     4021 cggcgcggcg gttgcctgtg ccgcctcatt ctggctgttt tttgccttca agaccgaaag
     4081 ctcctgccgc ctgtggcagc cgctcaaacg cctgccgctt tatctgcaca cattgttctg
     4141 cctggcctcc tcggcggcct acacctgctt cggcacgccg gcaaactacc ccctgtttgc
     4201 cggcgtatgg gcggcatatc tggcaggctg catcctgcgc caccggaaag atttgcacaa
     4261 actgtttcat tatttgaaaa aacaagggtt tccattatga aaatcatcct gaccacttct
     4321 atggcaggct tgggcggcac ggaaaccgcc accgtccgcc tcgggcggct actcaaacgg
     4381 cgcgggcacg acgtcatcct tgcttcttcg gacgggcctt ttgtcggaga agcgcaagca
     4441 tcgggcatcc gttggcagcc ggttgatttt taccggggcg ggcttgccgg ctacctcaaa
     4501 agcaccttta cctacgcccg gatgctgcgc cgcgaacaac ccgacatcat cgactgtcaa
     4561 atggcgcgcg tcgttcccgc ctgcgccctt gccgccaaaa tcgtttcgcc gaaaaccaaa
     4621 atcatctacc actcgcacgg tttggacgcg gcaacctacc ccaaaatcgc caaactcttc
     4681 gacaagttgg gcgtgtacat catcggcaac tgcaaacacg agcgcgaaaa actcatccgc
     4741 cacggcttcc ccgccggccg gattgcctac gcctacaacg c
//
LOCUS       DAXHSJ010000148         4715 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000148 DAXHSJ010000000
VERSION     DAXHSJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.148"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            288..986
                     /locus_tag="ACFM8U_001749"
     CDS             288..986
                     /locus_tag="ACFM8U_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6334949.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRIEH
                     VVWQKEMLPLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQNPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMHTID
                     LLADKVREEQKAAQ"
     gene            complement(1043..2047)
                     /gene="amgK"
                     /locus_tag="ACFM8U_001750"
     CDS             complement(1043..2047)
                     /gene="amgK"
                     /locus_tag="ACFM8U_001750"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC6334950.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2117..4525
                     /locus_tag="ACFM8U_001751"
     CDS             2117..4525
                     /locus_tag="ACFM8U_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC6334951.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
BASE COUNT         1133 a         1442 c         1241 g          899 t
ORIGIN      
        1 tatatttgaa aggggtgtaa cccttccgaa ccagggcaac gcatagggcg gtgctttatg
       61 tgccgtcctg tgtgttggaa catcattagt tccccaacag ccgtatccgt ctggggcgac
      121 ggcgcgtatt gtacgacaga aaccgcttca gacggcatcg ggtttgatgc cgtccgaagc
      181 ggtttcccca aacgaaaacg tccttttccg gcggcaaact gtatcaacac gcggaaatgc
      241 aataaaatac caatatccga tttatttcca acatcacgga gagccgtatg aaatccagac
      301 acctcgccct cggcgttgcc gccctgttcg cccttgccgc gtgcgacagc aaagtccaaa
      361 ccagcgtccc cgccgacagc gcgcctgctg cttcggcagc cgccgccccc gccggactgg
      421 tcgaagggca aaactatacc gtccttgcca acccgattcc ccaacagcag gcaggcaagg
      481 ttgaagtcct tgagtttttc ggctatttct gcccgcactg cgcccacctc gaacctgttt
      541 tgagcaaaca cgccaagtct tttaaagacg atatgtacct gcgtatcgag cacgtcgtct
      601 ggcagaaaga aatgctgccg ctggcacgcc tcgccgccgc cgtcgatatg gctgccgccg
      661 acagcaaaga tgtggcgaac agccatattt tcgatgcgat ggtcaaccaa aaaatcaagc
      721 tgcaaaatcc ggaagtcctc aaaaaatggc tgggcgaaca aaccgccttt gacggcaaaa
      781 aagtccttgc cgcctacgag tcccccgaaa gccaggcgcg cgccgacaaa atgcaggagc
      841 tgaccgaaac cttccaaatc gacggtacgc ccacggttat cgtcggcggc aaatataaag
      901 tcgaatttgc cgactgggag tccggtatgc acaccatcga ccttttggcg gacaaagtac
      961 gcgaagaaca aaaagccgcg cagtaagccc gtttgaaaaa tgccgtctga aacttggttt
     1021 tcagacggca ttttgattgg gtttaaaacg taaagcccgt ttccagttct tcatcgccga
     1081 ccagttcgac caagagcgcg tagagcgggg cgagttcggc ataacggcgc gatacgcggc
     1141 gcaggtaatt gaggaaacgc gggatttcgg gacggtattt gtctttgccg tcgcggtagt
     1201 acaggcgtgc gaagatgcct gcaaccttca agtgccgctg cacgcccatc cattcgaacc
     1261 agcggtaaaa ctcgtcaaac gcttcgggga cgggcaggcc ggcagcccgc gccttttccc
     1321 agtagcggat aaccaagtcc aagacaaatt cttcttccca ttcgataaag gcatcgcgca
     1381 acagcgacac caaatcgtag gaaatcgggc cgtaaagcgc gtcttggaag tctaaaacac
     1441 ccggcctgcc gcgcgtcagc atcaggttgc ggacgataaa gtcgcggtgc acatagactt
     1501 tgggctgcgc caacaggggc ggcagcagcg tatcgacggt ctgctgccaa agttggcgtt
     1561 gtttgaatgt taattcgcgc cccaattctt ttgcgacaaa ccattccggg aacaggttga
     1621 tttcgcgcag catcgtttca cggtcatatt cgggcaaaac cccttcacgg cttgccttct
     1681 gcaattcgac cagctcgccg atggcctcca aaagcagggc tttgtgcgcc gtttcgccct
     1741 gttcctgaag catcgcggtc aaaaacgtcg tattgcccaa gtcgttcaat accacaaacc
     1801 ccagatccgt gtccgcgtgc aatacctgcg gcacattgac catgtcaaac agtttctgca
     1861 ctttcaaata aggtgcgaca ctcatcttgt cgggcggtgc atccatgcag acgacactgc
     1921 tgccgtctga aaacgttgca cggaaatagc ggcggaaatc agcatccgcc gccgcaaaag
     1981 tcagatcgaa gtcccgttcg ggataaacgg tctgaagcca atttttcagt ttgatttgtc
     2041 gttgcataac agtactaaag catttcaggt tacaataaac gctattctaa ctggcaaacc
     2101 gacttgaggg gcgattttgg ctcgtttatt ttcactcaaa ccactggtgc tggcattggg
     2161 cctctgcttc ggcacgcatt gcgccgccgc cgatgccgtt gcggcggagg aaacggacaa
     2221 tccgaccgcc ggaggaagcg ttcggagcgt gtccgaaccc atacagccta ccagcctgag
     2281 cctcggttcg acctgcctgt tttgcagtaa cgaaagcggc agccccgaga gaaccgaagc
     2341 cgccgtccga ggcagcggcg aagcatccat ccccgaagac tatacgcgca ttgttgccga
     2401 caaggtggaa ggacagtcgc aggtgcaggt gcgtgccgaa ggcaacgtcg tcgtcgaacg
     2461 caaccggacg accctcaata ccgattgggc ggattacgac cagtcgggcg acaccgttac
     2521 cgcaggcgac cggttcgccc tccaacagga cggtacgctg attcggggcg aaaccctgac
     2581 ctacaatctc gagcagcaga ccggggaagc gcacaacgtc cgtatggaaa ccgaacacgg
     2641 cggacggcgg ctgcaaagcg tcagccgcac cgccgaaatg ttgggcgaag ggcattacaa
     2701 actgacggaa acccaattca acacctgttc cgccggcgat gccggctggt atgtcaaggc
     2761 cgcttccgtc gaagccgatc gggaaaaagg cataggcgtt gccaaacacg ccgccttcgt
     2821 gttcggcggc gttcccattt tctacacccc ttgggcggac ttcccgcttg acggcaaccg
     2881 caaaagcggc ctgcttgttc cctcactgtc cgccggttcg gacggcgttt ccctttccgt
     2941 tccctattat ttcaaccttg cccccaatct cgatgccacg ttcgcgcccg gcgtgatcgg
     3001 cgaacgcggc gcggtctttg acgggcaggt acgctacctg cgaccggatt atgccggcca
     3061 gtccgacctg acctggctgc cgcacgacaa gaaaagcggc aggaataacc gctatcaggc
     3121 gaaatggcag catcggcacg acatttccga cacgcttcag gcgggtgtcg atttcaacca
     3181 agtctccgac agcggctact accgcgactt ttacggcaac aaagaaatcg ccggcaacgt
     3241 caacctcaac cgccgtgtat ggctggatta tggcggcagg gcggcgggcg gcagcctgaa
     3301 tgccggcctt tcggttctga aataccagac gctggcaaac caaagcggct acaaagacaa
     3361 accgtatgcc ctgatgccgc gcctttccgc cgattggcgc aaaaacaccg gcagggcgca
     3421 aatcggcgtg tccgcacaat ttacccgttt cagccacgac agccgccaag acggcagccg
     3481 cctcgtcgtc tatcccgaca tcaaatggga tttcagcaac agctggggtt acgtccgtcc
     3541 caaactcgga ctgcacgcca cctattacag cctcaaccgc ttcggcagcc aagaagcccg
     3601 acgcgtcagc cgcactctgc ccattgtcaa catcgacagc ggcgcaactt ttgagcggaa
     3661 tacgcggatg ttcggcggcg gagtcctgca aaccctcgag ccgcgcctgt tctacaacta
     3721 tattcctgcc aaatcccaaa acgacctgcc caatttcgat tcgtcggaaa gcagcttcgg
     3781 ctacgggcag ctttttcgtg aaaacctcta ttacggcaac gacaggatta acaccgcaaa
     3841 cagcctttcc gccgccgtgc aaagccgtat tttggacggc gcgacggggg aagagcgttt
     3901 ccgcgccggc atcggtcaga aattctattt caaggatgat gcggtgatgc ttgacggcag
     3961 cgtcggcaaa aaaccgcgca accgttccga ctgggtggca tttgcctccg gcagcatcgg
     4021 cagccgcttc atcctcgaca gcagcatcca ctacaaccaa aacgacaaac gcgccgagaa
     4081 ctacgccgtc ggtgcaagct accgtcccgc acagggcaaa gtgctgaacg cccgctacaa
     4141 atacgggcgc aacgaaaaaa tctacctgaa gtccgacggt tcctattttt acgacaaact
     4201 cagccagctc gacctgtccg cacaatggcc gctgacgcgc aacctgtcgg ccgtcgtccg
     4261 ttacaactac ggttttgaag ccaaaaaacc gatagaggtg ctggcgggtg cggaatacaa
     4321 aagcagttgc ggctgctggg gcgcgggcgt gtacgcccaa cgctacgtta ccggcgaaaa
     4381 cacctacaaa aacgctgtct ttttctcact tcagttgaaa gacctcagca gtgtcggcag
     4441 aaaccccgca gacaggatgg atgtcgccgt tcccggctat atccccgccc actctctttc
     4501 cgccggacgc aacaaacggc cctgaccgtc ggaaacccgg caggagcacc gttcccgcac
     4561 aagacggcat tccaccgaca accccaaacc cgccatcaaa ggcaggattc aaacgataag
     4621 gaaagaatga tgaaaatcaa agccctgatg attgccgccg cattgctggc agcagccgat
     4681 gtccacgccg caccgcaaaa ggcaaaaacc gcatc
//
LOCUS       DAXHSJ010000149         4612 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000149 DAXHSJ010000000
VERSION     DAXHSJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.149"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..474
                     /locus_tag="ACFM8U_001752"
     CDS             <1..474
                     /locus_tag="ACFM8U_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC6334952.1"
                     /translation="EFSDYQRVYLDETGFDRYLFRPYTRSLKGQIVKAQISGKRYQRL
                     SLVSAQVGNRLIAPMVYQNTMIGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLR
                     EMAEKLGHKVLPLAPYLPELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
     gene            complement(638..2041)
                     /locus_tag="ACFM8U_001753"
     CDS             complement(638..2041)
                     /locus_tag="ACFM8U_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFC6334953.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTGLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(2110..3537)
                     /locus_tag="ACFM8U_001754"
     CDS             complement(2110..3537)
                     /locus_tag="ACFM8U_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFC6334954.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLYDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3879..4115
                     /locus_tag="ACFM8U_001755"
     CDS             3879..4115
                     /locus_tag="ACFM8U_001755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334955.1"
                     /translation="MPTKNLGKILSGSLTAAAILMLFVWIIPKQFGPSKEEIQAQEEW
                     DRKYKEAEAVFNEQCKTAGERFTRRRTMWKGLCC"
     gene            4106..>4612
                     /locus_tag="ACFM8U_001756"
     CDS             4106..>4612
                     /locus_tag="ACFM8U_001756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334956.1"
                     /translation="MLLKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFS
                     DGEYRYVDVLQPNHSDIIRYSGKDFPINQTLNHIHPARYAVTFENNVDSKLRRHWVAG
                     ATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAILCNKERLTSSEPLSDFVL
                     SVLKPYILR"
BASE COUNT         1098 a         1199 c         1129 g         1186 t
ORIGIN      
        1 gaattttccg actaccaacg tgtttatttg gatgaaacag gatttgaccg ctacctgttc
       61 cgtccctata cccgcagcct gaaagggcaa atagtaaaag cgcagataag tggaaaaaga
      121 taccaacgct tatctttggt gtccgcacaa gtcggcaacc ggctgattgc tccgatggtt
      181 tatcaaaata cgatgatcgg agtctttttt gaagcgtggt ttcagcaatg cctactgccc
      241 gcattgactc aaaaatcggt gattatttta gataatgcgc gatttcaccg tatgggtgtc
      301 ttacgggaaa tggcggaaaa attgggacat aaggtattgc ctcttgcacc ttatttacct
      361 gagctcaacc cgattgagaa agtgtgggcg aatattaagc ggtatctgag aaccgttttg
      421 tctgattacg cccgatttga cgatgcacta ctgtcctatt ttgattttaa ttgaatatac
      481 atttgaatta attcgcactt aatttaaatg tgtttttaac tgtgctttat ttaaaggcaa
      541 tgagaatgtg aaagtatcgg atcaatccca aagcagcctg cactttcgaa acggggtgca
      601 ggctgctttg ggaatttcat aaccgtttca gcctgcttta ttccgcaaat accgtttcca
      661 accctaaccc gctctctttc accaagcgca aataagccag catgaattta taccgtgctt
      721 gagccagttt ctgttctgct tgggcgactt cctgccgcgc ccgtattact tccagccggt
      781 tgcggatgcc gtattgttgg ccggtttcgg tcgatttcag tttcaaacgg ctgctttcca
      841 aaacccgttc ttgcgccatg atttggtaac gcgccgcacc gctttcggta taagcctggc
      901 gtacggcgag tttgatgtgc cgctcggttg cggtcagctg tgcttcggcg gccccgtatt
      961 gcgcttcggc ttcatggatt ttgcccgaca attctccgcc ggtataaagc ggcaaattca
     1021 actgtacgcc gacgctcatc cctttgcccc gatagtggta gtcattattc tgcgcagatg
     1081 aagtgtagag gttattctga tagccgacat gggcagaaac ggtgggatag cggctgttct
     1141 gtgctgcccg aagcgcctgt ccgctgcttt gcagggcaag ctgctgcatc cggtattcat
     1201 gattgttgga taaggcaatg cgctgccatt catccagact gtaacgttcc agcttgggca
     1261 gatagcgtgc caacaggttg gcggtatcta tggcctcgat ttgtttgctg tccaggccgg
     1321 tgtagtcgtt caactggttt tcataggttt gtttctcagc caatacggcg atttcttggg
     1381 ccagggcatt gtcgtaaccg gctttggctt cgtgaatatc cagcgcggtg gcagcacctt
     1441 tattgaataa agcctgcgcc tgccttacct gctgggcata agcctctttt tccgccgcat
     1501 gggcggcaac ggtgtctcgg ctgagtaaaa cgttgaaata actttcggca actttcaaca
     1561 gcaattcttc gcgtgccgca tcgaaacgct gttctgcagc ctgcgtatcg aacctgcttt
     1621 ggcggtattg tgcaaatttg gcagcgtcaa ataaggtttg tcccacctgc acgctccatc
     1681 cctgtgtttc gcgggtggaa gaaatcgatg gcggctggcg ctggtagctg gcattggcgg
     1741 atacatgggg aaggaatgcg gccttggctt gttgttgccg tgcgcgcact gcatcacgct
     1801 ggtaatggga cgcttgaaaa tcagccgaat gttgctgcgc cgcccgccat gcttcaggca
     1861 gcgtaaaagc cgaaacggat ggggaaaggg atagtggcaa ggtaaaaagt gaaacgggta
     1921 ggatatattt ggaaaaatag gatttcatag ccgaaaatag ttcatgttgc aaatagggcg
     1981 tcagtgtcag gcaaacggaa ataccgtaat cttgcattat cattagattg agcaatgtca
     2041 tccgggcaat ggtttcaggc agtctgcatg tccgaaccgg cggataacaa atgcccagta
     2101 tggatccgcc tatcgctccc taaagctttc gtccaatttg gtttgcagcg ggcttaacag
     2161 ataatccagc acccgccgtt tacccgtttt aatctccgcc gtgacattca tgcccgccgt
     2221 cagattcact gctttgccgt caatattcaa ggtatgtttg tccagcgaca ccaccgccgt
     2281 ataaaccaag cccaactgtt cgtggcttac cgcatcatgg ctgacacttt tcaccttgcc
     2341 cgtcagataa ccgtagcgcg tatagggaaa actctcaatc ttcaccaccg catcctgtcc
     2401 ctgttccaca aaaccgatgt ctttgttcaa taccaaaact tccacgtcca ttttgtcgtc
     2461 atcgggcgca accaccatca ttttttgggc agcctgcacc acaccgccca ccgtataggt
     2521 ggccaattcc tgcaccgtgc cgtccgcagg cgactgtatt gtcatcagct gctgccgctg
     2581 ctttgcctta tccgtttggc cgcggtattg gtcaatctgt tcgtttgcct ggcgcagcgc
     2641 atccagcgta tcgcgtttca ggttctgcgt attcagcacc cgactctgct ccgcctgtgc
     2701 aatggccgcc tgaatctgcc tcatctgacc gcgcgtactt tccaaatcgt tccaattgct
     2761 gaccgatttg ctctgctgct ccaaaaacgc atgttccgaa ataaaattgt cggcccgcaa
     2821 acggcggtag tctgctgttt tctgctgctc gatcgccccc accgaaacca gcttctgctc
     2881 ctgcgccttg gccgactgca attccgcctg atggccgcgc aaagccgact gcaattgcgc
     2941 atcctgcgcc gcccatgcct gatactggtg ctgcgccaac acctgcgccg attgcacatc
     3001 ggcatcgtag agacctaaag accgtgcttg cgccatatcg atatgcggca cggtacggct
     3061 ttccaatgcc gccaatactg cttcataacg cagtttggac aattgggcag cctgcaaagc
     3121 ctgctccgac tgcaccacat cgctgtctgt tcccacagcc tccagttccg ccagcgtttc
     3181 tccctgtttc acatgctgcc cgtcgcgcac atgtaccgcc ttaaccaccg ccgtttccag
     3241 cggctggatg gttttgctgc gcccgcccga caccgttttg cccgaagccg ccgccacaat
     3301 atcgattttg ccgaaccagg accacaacaa agccaaaagc gcaaacgcca taataaaacg
     3361 cgccgcccat ttcggagcgg cagagaccgg cgtatcggtc agttccaaat gcgcgggcaa
     3421 aaacgcctgt tcttccgccg tgcgtttggg cggttccaac tggtcgcgca ccgcccaaac
     3481 attgcgccat acagtaatgt agcgagaaag aaaggatttc agggcggaga aaaacataac
     3541 gggtataacc ttggcaatat agaaacagga aacaatataa atatgtaaag gaattttaac
     3601 ggaaagcgcg gcagctgtta agggaaaggc gggaatattg acaaaaaata cccaagtcgt
     3661 tacaaatatt cattatttta ctgcgtaacg caacgctgaa gcgcaggctg cttttgagat
     3721 gcggcaaggt tcggcaaaaa gcagcctgca catttaacca caggaactac ccatgtttac
     3781 cacaaacgat ttacgccatt tcctagaagg tttggtcatc ctattcgcaa tcggctattg
     3841 ggacaccatg ctgctgttgc tttggtttct cgtccgcttt gcctacaaaa aacctcggca
     3901 aaatcctttc aggcagcctg accgccgccg ctatcctgat gctgtttgtt tggattattc
     3961 ccaaacaatt cggcccgagc aaagaagaaa tacaggcaca agaagagtgg gacagaaaat
     4021 acaaagaagc cgaagccgtg tttaacgaac aatgcaaaac ggcgggggaa agatttacca
     4081 gacggcggac aatgtggaag ggattatgct gttgaaggta gtacctgagc gtaccgtttc
     4141 ggcagatgca aaaaccagag acccgatgtg ggacaatgcg gctttacaga ccagcgaagg
     4201 cgtaaatttt attgctcgtt tcctaggatt ttttagcgat ggggaatacc gctatgtgga
     4261 tgtcctgcaa cccaaccatt ccgatattat tcggtattca ggtaaagatt ttccgataaa
     4321 tcaaacactt aatcatatac accccgcccg ttatgcggta acgttcgaaa acaatgtcga
     4381 ctccaagctg cgcaggcact gggtggcagg tgcgaccata cggattatcg accgccaaac
     4441 tgacgaagtg attgccaaga aaaccatcta tgtctttgaa aaaggcttgg acggcacggg
     4501 tggggcgaga atgccgtgga agtttgctat cttgtgcaat aaagaaagac ttacttcttc
     4561 agagccgtta tcggattttg ttcttagcgt tttaaaacct tatatattgc gt
//
LOCUS       DAXHSJ010000150         4589 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000150 DAXHSJ010000000
VERSION     DAXHSJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.150"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..128)
                     /locus_tag="ACFM8U_001757"
     CDS             complement(<1..128)
                     /locus_tag="ACFM8U_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6334957.1"
                     /translation="MKEYKVVIYQESQLSSLFFGAAKVNPVNFSAFLNKQTPEGWRV"
     gene            complement(203..1546)
                     /gene="argG"
                     /locus_tag="ACFM8U_001758"
     CDS             complement(203..1546)
                     /gene="argG"
                     /locus_tag="ACFM8U_001758"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC6334958.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            1873..2013
                     /locus_tag="ACFM8U_001759"
     CDS             1873..2013
                     /locus_tag="ACFM8U_001759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334959.1"
                     /translation="MQTAALKQTLPEKRKLDPLPNPLPWEREQITAPSTFSDGIGKET
                     HL"
     gene            2010..2810
                     /locus_tag="ACFM8U_001760"
     CDS             2010..2810
                     /locus_tag="ACFM8U_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC6334960.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     VTRKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYVGRFNQTKRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            2850..3836
                     /locus_tag="ACFM8U_001761"
     CDS             2850..3836
                     /locus_tag="ACFM8U_001761"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC6334961.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            4493..4589
                     /locus_tag="ACFM8U_001762"
                     /pseudo
     CDS             4493..4589
                     /locus_tag="ACFM8U_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1071 a         1181 c         1276 g         1061 t
ORIGIN      
        1 acccgccagc cttcgggggt ttgtttgttg aggaacgcgc tgaaattgac ggggttgacc
       61 tttgccgcgc cgaaaaacag gctggacaac tggctttcct gataaatgac gactttgtat
      121 tctttcatag gattcctttt aacataaggg atcatctttt cagacgaccc cttaagttgc
      181 taatgatgca aaacagcaaa ccttattgct tattgcccaa ctgcggtaat accgagcctt
      241 cgcccagcga cagcaaaccg ctttgcgagt agatgcccag tttggcgcgg gtgtcggtga
      301 tgtcgaggtt gcgcatcgtg agctgtccga tgcggtcgag cggggtaaac gccgcacctt
      361 cgactttttc catactcagg cgttcgggtt ggtaggtcag gttgggcgat tcggtgttca
      421 gaatcgagta gtcgttgccg cgccgcagtt cgagggtgac ttcgccggta acggctttgg
      481 cgacccagcg ttgggcggtt tcgcgcaaca tcaaggcttg gctgtcgaac cagcggcctt
      541 ggtagagcaa acggccgagg cgcaggccgt tgatgcggta ttgttcgatg gtgtcttcgt
      601 tgtggatgcc ggtcaccaag cgttcgtagg cgatgtggaa caacgccata cccggggctt
      661 cgtagatgcc gcgcgatttg gcttcgatga tgcggttttc gatttggtcg ctcataccca
      721 agccgtggcg gccgccgatg cggttggctt cgaggaagag ttcgacgggg tcggcgtatt
      781 ctttgccgtt cagtgcaacc ggcacgcctt cttcaaagcg cacgctgact tcttcggggc
      841 tgacttcaac gttttcgtcc caaaaggcaa cgcccataat gggtttgacg attttgatgc
      901 ccgagttcaa aaattccaag tctttggctt cgtgggtcgc gccgagcata ttggaatcgg
      961 tggagtaggc tttttcaacc gacattttgt agttgaagcc gttggcaatc agaaattcgc
     1021 tcatttcgtg acggccgccg agttcgtcga taaattgctg gtcgagccag ggtttgtaga
     1081 ttttcagcgc gggattggtg agcaaaccgt agcggtagaa acgctcgatg tcgttgcctt
     1141 tgtaggtgct gccgtcgccc cagatgttta cgtcgtcttc tttcattgcg gaaacaagca
     1201 tcgtgccggt tacggcgcgg cccagaggcg tggtgttgaa ataggcgatg ccgccggtgg
     1261 aaacgtggaa cgcgccgcat tggatggcgg cgatgccttc gtgtgccaac tgcgcgcggc
     1321 agtcgattaa gcgggcgttt tccgcaccgt attccatcgc ttttttggga atggcgttgt
     1381 agtcgtcttc atcgggctga ccgaggttgg cagtgtaggc ataaggcagt gcgcctttga
     1441 gtttcatcca caacagcgcg gcggaggtat caagtccgcc ggagaaggcg atgccgactt
     1501 tctgaccgac ggggaggttt tgtaaaatag tatggttttg gctcataagg gtatccttcg
     1561 gacttggaaa aaggagttcg tcgtgtttcc ttttcgattg aaaccccgcc acttggacat
     1621 ctgtccttcg gggcggtaga atcagacttt atttgggagg ggcgtaatcc cttccaaatc
     1681 aggacggcac atagggcggt gctttatgtg tcgtcccgtg tgttgaaaca ttatcttata
     1741 aatataaccc attgtcagcc gttaaggctg tctgaaaaga agctttgccc gtttcgctga
     1801 aaatcttttc agacggtctt tacgccatcc tcaacctgac tccctcccct gcgggggagg
     1861 gttggggaga gggtgcagac agcagccctt aagcaaacgc tgcctgaaaa acgcaagctc
     1921 gaccctctcc ctaaccctct cccatgggag agggaacaaa tcacagcacc atcgacattt
     1981 tcagacggca tcggtaaaga aacccatcta tgaacgcgtt caacctcacc atcatcggcg
     2041 acgaaatctt acacggcagc cgccaagaca agcatttcgc ctttttcaaa tccctgctcg
     2101 aatccaaagg gctgaagctc aatcaggtgc aatatctgcc cgacgaaccc gatttgctgg
     2161 tcaggcaact gcgccgcagc ttttcagacg gcataccgac gtttgttacc ggcggcatcg
     2221 gcgccacgcc cgacgaccac acgcgccaag ccgcagcggc tgctttggat ttgcctgtcg
     2281 tccgccatcc tgaagccgcc aagtttatcg aaggcgtaac ccggaaacgc ggtgagccgc
     2341 tcgattcgcc cgaacacgcc caacgcctga agatggcgga ttttccccaa ggcgcggaat
     2401 tggtgcctaa tccgtttaat aatatcgccg gattttccat tcgcgagcat tatttcttcc
     2461 ctggttttcc tgtgatggcg cacccgatgg cggaatgggt tttggatact tattacgtcg
     2521 gccgcttcaa ccaaaccaaa cgcggcagcc gcagcgtgta tgtgtttgag cagcccgaat
     2581 cgcgcattac gccgcttatg gagcatatcg agcaaactta tcccggtgtg cgttcgtaca
     2641 gcctgcccag cgtcggttgg acgcattcag acggcacgca ggtcaaaccg cacattgagt
     2701 tcggcatcaa agccgaagga gaagccgtca accttttgga cgcggcgtgg gcggaagtgt
     2761 tgcacagctt ggacggattg ggagcagagc tgaaaaatcg ggtaaactga aaaccgtttg
     2821 cccaaatcgg acgatattta aggaaaacca tgcaataccg aatcagaaga gaaaacgaag
     2881 cacccgaagc aaaaaatacg ggggaaacct tatgggagcg cgacatcatg cgcgaagtgc
     2941 tgctgtccgc ctatcaggac aggcgcaggg agcggatgtg gaaaaacatc tggcgtgccg
     3001 tcagcaccct gattctggtt gccctgattg ccggtatttt ccgaaaagac gaagccgcat
     3061 tgcagttggc gggcaacacg ccgcataccg cagtcgtcaa cctgtacggc gaaatcggca
     3121 acggtgtaga agaccaggtc aaaaaactca aagacggtat ggaggccgcc tacaaaaacc
     3181 cgcaggcaaa agccatcgtc atccgcgcca acagccccgg cggttcgccc gtcgtctcca
     3241 acaccgcttt tgaagaaata cgccgtctga aggcgcagca ccccggcatt cccgtttatc
     3301 tcgtggcgga agatatgtgc gcgtccggct gctactacat cgcggcggcg gcggacaaaa
     3361 tctatgccga cccgtccagc atcgtcggca gcatcggcgt tatcggcagc agtttcgacg
     3421 cgaccggcct gatggaaaaa atcggcgtga aacgcagggt taaaattgca ggcagcaaca
     3481 aaggcatggg cgatccgttt tcgcccgaaa cgcccgaaca gtcgaaaatc tgggaagaaa
     3541 tgctgaccgg catacacggc gaattcatca aagccgtcaa aaccggacgg ggcggccgcc
     3601 tcaaattccg acaatatccc gacgtgttca gcggtcgcgt gtacacaggc gcggatgcgc
     3661 tgaaagtcgg gctggtggac ggactcggca atatttacag cgtggcgcga gacgtggtca
     3721 aagcccccga tgtggtggat tacactccga aggacgattt cggcagaatc ttgggcagac
     3781 gcttcggcgc agaactcaaa gcaagcgtgc gcgaagccct gcaggccgtc cggtagcgtt
     3841 gtttacagta tttctaaggg aataccaaaa taaaaatata cacaaaaaca ctcacctccc
     3901 cagtatgctg ccactaagtc agccgcgtca ctatttaccg tgcacttaaa gccgtaaaag
     3961 gcaggttgtc cccgggaggt tttgtgcatc ctgataccga tcggttgttg ttgctcaaag
     4021 gacagaaggc cgctgataaa cgagattacc tgtttgtcgc tattgacgat ttttatactc
     4081 tgccattttg ccagacaaaa ccgcagacag tgctgccaag tttctgaccg aacatctggc
     4141 cgacccctgc ttgtacctga ttgagtacgt ttactctgac aatgataggt aatataaaga
     4201 gccgtccaac atgctttcgg tgcagcttgt tataataatg ggattggttg gaggcttgcc
     4261 cgatttgctt gtccgcagac caacggtaag gcggagcggg ttatccgtac cttgatggag
     4321 atgtggcatg aggaacagtc gtttgacagt ccggaacatc ggcaaaagga gttgtgctgc
     4381 tttattaatt taggggctgc cctagatcac tagggaaatt caaattaagt tagaattatc
     4441 cctatgagag aaagtcgtct aagccggtat aaacaaaata aactcatttt gttatcgtgg
     4501 ctatgatgta ttggatgcgg gcgaatttag ccatttccgt atcaatcaca gcacacattt
     4561 tgccgaacga caaaaccata ttaatggaa
//
LOCUS       DAXHSJ010000151         4477 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000151 DAXHSJ010000000
VERSION     DAXHSJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.151"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            417..492
                     /locus_tag="ACFM8U_001763"
     tRNA            417..492
                     /locus_tag="ACFM8U_001763"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:451..453,aa:Glu,seq:ttc)
     gene            1259..2065
                     /gene="thiD"
                     /locus_tag="ACFM8U_001764"
     CDS             1259..2065
                     /gene="thiD"
                     /locus_tag="ACFM8U_001764"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC6334962.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCKAVRTAKSYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            complement(2115..2984)
                     /gene="tehB"
                     /locus_tag="ACFM8U_001765"
     CDS             complement(2115..2984)
                     /gene="tehB"
                     /locus_tag="ACFM8U_001765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC6334963.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKLKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAARDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            3098..3535
                     /gene="rnhA"
                     /locus_tag="ACFM8U_001766"
     CDS             3098..3535
                     /gene="rnhA"
                     /locus_tag="ACFM8U_001766"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC6334964.1"
                     /translation="MDQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT         1131 a         1280 c         1086 g          980 t
ORIGIN      
        1 cgtctgaaaa tttaaaagtt ctagattccc gctttcgcgg gaatgacgaa gtttcagacg
       61 gcatcgcccg cctgttttga tatggcggca cccccccccc gacaaaaaaa acaatccgga
      121 acgcatctga ccgttccggc ttattttcag gcgaatccgc cgcatcagaa catactgcgc
      181 acgcccatat tgacctgcca agtctggcgc atcgtgtgca tcgaagacct ttgcgcctca
      241 aaataaagct gccttccgtt gtcggcatta ccacgcaaaa aaatgaattg cttgatattc
      301 caatgttttt atatgttttt atatggtgat gcgatcagac aaacgccccc ctgacatttg
      361 tttagacggc atcgtattgc taaatttcta taagtatgta taatgtccgt ttccacgcgc
      421 ccatcgtcta gaggcctagg acactgccct ttcacggcgg caaccggggt tcgaatcccc
      481 gtgggcgtgc caattcaaaa acctgcttgt ttcaagcagg tttttttatt atgaatcgtc
      541 attcccgcac ccttcgtcat tcccgcgaaa gcgggaatct ggagcgtagg gttgaagaaa
      601 ccgttttatc cgataagttt ccgtgccgac aggtctggat tcccactttc gtgggaatga
      661 cgagtctttt ataaccttta aggctgctct gcccaatggt ttggattccc gcttatgcgg
      721 gaatgacggg gcggggcgat gccgtctgaa acttcgtcat tcccacaacc cttcgtcatt
      781 cccatgaaaa cgggaatcta gaacttctga attttcagac ggctttataa atattgccgc
      841 tgccccaagg gctagattcc cgcctgcgcg ggaatgatga tttggaaatc acccgaaacc
      901 caaaaacaac tgaaaccgaa cacactggat tcccgctttc gtaggaatga cggaatttta
      961 ggtttctgtt tttgtgggaa tgacgaataa agcgtgccgg tttatgctca ccgcaacacg
     1021 cggttcagac ggcattgctc tcttttttca ttatcagtgg gtgtagcaac tgtatttttc
     1081 accccgtcgg gcaaaaatac agttgctacg atgcaccccg ccgccctgcc ctgtgccttg
     1141 tcctgcaata cggcatataa tgcaccacaa accccgcgct gcggttttca gacggcatcg
     1201 ccgtgctttt ttacaggcat tagccctttt tatcggacac aatattaagg aggaataaat
     1261 gaaaagctct tttgtgcaaa cgcttaccat cgccggttcg gattcgggcg gcggtgcggg
     1321 cattcaggcg gatttgaaaa cgtttcagat gcgcggcgtg ttcggaacgt gcgtcatcac
     1381 cgccgttacc gcgcaaaata ccttgggcgt gtcggcggtt catctcgtcc cgaccgaaac
     1441 catcaccgca caaatccaag caatcaggga agactttgac atccgcgcct acaaaatagg
     1501 aatgctcggc acggcggaaa tcatcgaatg cgttgccgac aagctgaaac actgcgactt
     1561 cggcaggcgc gtactcgacc ctgtgatgat tgccaaaggc ggtgcgccgc tgttgcagga
     1621 ttccgccgtt gcggcactga cgcgcctgct gcttcccgat acggatgtat tgacccccaa
     1681 cctgcccgag gcggaagccc tgaccggcgt gcatatcgaa aaccgtaaag atgcggaacg
     1741 tgcggcaaaa atcctgcttg attacggtgt caaaaatgtc gttatcaaag gcggacattt
     1801 gaacggcagc acaagcggac gctgcacgga ttggctgttt acgcaaaatg aaacgctgga
     1861 attggacagc ccgcgctttc caaccgccca cacgcacggc acgggctgca cgttttccgc
     1921 ctgcatcacc gccgagttgg caaaaggctc ggacgtttgc aaagccgtac ggactgccaa
     1981 gtcctacatt acggcggcaa tctcaaaccc tttggaaatc ggcgcgggac acggcccggt
     2041 caatcattgg gcgtatcggg actagacgta aaaatgccgt ctgaaacaaa atgttcagac
     2101 ggcatttttg aggattattc aggctttttc gccagcatgg ttacaaattt aaaccgtatc
     2161 ggattgccgt tttcgtcttt ggcgtgcatc gcgcccaatt cttctttata ttcgaccagc
     2221 tcccaatccc gataataatc cttcagctct ccctctttaa atttaaaagg gaacggcatc
     2281 ggacaaggga aatccgccgt atccattgcc gacacaatca aattgtaccc gcccgccgcc
     2341 gtatgcgcct gcatatcggc aatcacgtcg ggcacgcgct gcggcatcag gaacatcagc
     2401 accactgttg ccacaatata atcaaactca ccctgcaagg cggcggcatt caagtcatat
     2461 tccagcgtgc ggacgttcaa gccctccgcc tctgccagct ccgccacgtt tgccaaggcg
     2521 gccggattgt gatcgactgc agtaacttca aatcccttca aaccgagaaa cagtgcgttg
     2581 cgcccctgtc cgcagcccat atccaacgcc ctgcccgccg gtacggtatc ccgtgccgcc
     2641 gcgaccgcag aatgcgtggc actcatcccg tattttttgt gaaaatagtc tgccgccgcg
     2701 caatacagcg acaaacggat ttcggcatcg tccgttttca gtttgaccga aaacacctgc
     2761 tgcggcgcaa acacgcaatc gccgctttcc gccgaccaaa cctctgccgc cccgtcctgc
     2821 gtgcggactt cgacatcgcc ctgcaacaca ttcaggcaga cccattcccc tccttcggac
     2881 gaataacccg acaacaaaat ttccggcagg ttttccgcct tccatacggg catttgtccg
     2941 aaacaaaaca actcgccgct ttgacccact atccgctcct tcatattcaa aaataaagtt
     3001 gcacattata tgcctatttt aatccgccgc aatctttcag acggcacggc gcacaaaccg
     3061 cttataatca cgccggacac cacacaaagg cacaataatg gaccaaaccg tttaccttta
     3121 caccgacggc gcgtgcaaag gcaatcccgg cgcgggcggc tggggcgtgt tgatgcgcta
     3181 cggcagccac gaaaaagaac ttttcggcgg cgaagcgcaa accaccaaca accgcatgga
     3241 gctgaccgcc gtcatcgaag gactgaaatc gctcaaacgc cgctgcaccg tcatcatctg
     3301 caccgactcg caatacgtca aaaacggcat ggaaaactgg atacacggtt ggaagcgcaa
     3361 cggctggaaa accgccgcca aacagcccgt caaaaacgac gacttgtgga aagaactcga
     3421 cgttctggtt gggcagcacc aagtcagctg gacttgggtt aaaggacacg cgggacacgc
     3481 ggaaaacgaa cgcgccgacg atttggcaaa ccgtggcgca gcgcagtttt cctgactgcc
     3541 gctccggcaa aaatgccgtc tgaaaccgct aatgggcttt agacggcatc gtcctccacc
     3601 gtcattcccg cgcaggcggg aatccaaacc gtcgggcaac ggcaatattc aaaagccgtc
     3661 tgaaaattta gaggctctag attcccgctt ttgcgggaat gacgaaaagt tgcggacagc
     3721 agaggaatga cgaggtttca gacggcattg ggggctttgt cgggcttcag gcggcatccg
     3781 accgccccgt cattcccgcg aaagcgggaa tctaaaaacc caacgctgca aaaatttatt
     3841 tagaaacaac tgaaaccgct ccaccgtcat tcccgcgcag gcgggaatct agaccgtcgg
     3901 gcaacggcaa tattcaaaga ttatctgaaa gtttgaagtt ctagattccc gttttcacgg
     3961 gaatgacgaa aagttgcaag aatgacggag tttcaggcgg catccgaccg ccccgtcatt
     4021 cccgcgaaag cgggaatcta aaaacccaac gctgcaagat ttatcagaaa caactgaaac
     4081 cgaacggact ggattcccgc ctgcgcggga atgacgggat tttagtaacc gtagcaaccg
     4141 cctgcgcgac ggctaagggg cttcagcaac cgtagcaact gcctgtgtgg gaatgacgga
     4201 caatgggctt cagacggcat cagacggcat ctcttgcctg ccgctaaaac agtttgccgc
     4261 acaactgttc aaacgcgtcc gatatatttc aacacacagg acgacacata aagcacctcc
     4321 ctatgtgtcg tcctgatttg gaaggggtta caccccctcc caaataaagt ctgatcctgc
     4381 cgccccgaag gacagatgtc cgagtggcgg ggtttcaacc gaaaaggaaa tacgatgaag
     4441 tggtacaatt agcggcaatg cggacagaca aattaaa
//
LOCUS       DAXHSJ010000152         4421 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000152 DAXHSJ010000000
VERSION     DAXHSJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.152"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            38..461
                     /locus_tag="ACFM8U_001767"
                     /pseudo
     CDS             38..461
                     /locus_tag="ACFM8U_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            567..1130
                     /gene="pgsA"
                     /locus_tag="ACFM8U_001768"
     CDS             567..1130
                     /gene="pgsA"
                     /locus_tag="ACFM8U_001768"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC6334965.1"
                     /translation="MPWNLPIFLTWLRVLLIPVLTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1210..1285
                     /locus_tag="ACFM8U_001769"
     tRNA            1210..1285
                     /locus_tag="ACFM8U_001769"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Gly,seq:gcc)
     gene            1311..1386
                     /locus_tag="ACFM8U_001770"
     tRNA            1311..1386
                     /locus_tag="ACFM8U_001770"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Gly,seq:gcc)
     gene            1410..1485
                     /locus_tag="ACFM8U_001771"
     tRNA            1410..1485
                     /locus_tag="ACFM8U_001771"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Gly,seq:gcc)
     gene            1507..1582
                     /locus_tag="ACFM8U_001772"
     tRNA            1507..1582
                     /locus_tag="ACFM8U_001772"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1540..1542,aa:Gly,seq:gcc)
     gene            1608..1681
                     /locus_tag="ACFM8U_001773"
     tRNA            1608..1681
                     /locus_tag="ACFM8U_001773"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1640..1642,aa:Cys,seq:gca)
     gene            1784..1873
                     /locus_tag="ACFM8U_001774"
     tRNA            1784..1873
                     /locus_tag="ACFM8U_001774"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1818..1820,aa:Leu,seq:taa)
     gene            1977..3029
                     /gene="sohB"
                     /locus_tag="ACFM8U_001775"
     CDS             1977..3029
                     /gene="sohB"
                     /locus_tag="ACFM8U_001775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC6334966.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3080..3949)
                     /locus_tag="ACFM8U_001776"
     CDS             complement(3080..3949)
                     /locus_tag="ACFM8U_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFC6334967.1"
                     /translation="MWKMLKHIAQTHRKRLLGTFSLVGLENLWMLVLYPVFGGRAINA
                     VIAGEVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSA
                     VTARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLL
                     PRFAAISENLYFRLKNSLERDNHFIRKGDRRQLYRHYGLLARLRVLISNREAFGYLCV
                     GTAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQ
                     RIGGLERNIKAGT"
     gene            complement(3959..4390)
                     /locus_tag="ACFM8U_001777"
     CDS             complement(3959..4390)
                     /locus_tag="ACFM8U_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFC6334968.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
BASE COUNT         1087 a         1051 c         1164 g         1119 t
ORIGIN      
        1 tcgctgggta tggtttgccg ccataagctg tctgctgttg tccgccgttt ttattgcacc
       61 ttacctgacg gcatttcacg aacaagaaaa aacattagaa tatgcggaat tgaccgttac
      121 cgcccccaac cgcagcggac gggcaatcaa actggatgca gacgggcagc attaccggct
      181 ttcctgctat gggttcgaca gtttgtgcac aggcggcaat atcggcagaa ccatcagggc
      241 ggagcaggtt agagccgttt taagcgaaac agtcggcaaa ggttttttaa acggcgttct
      301 gttggaatac cgcaacagtg gcagtgtcta taagcaataa agattttgcc cgcacggaag
      361 accgccttgt cgaagtgttg gcacaacctg ccgttttcag cctgaaactg ggtattttgc
      421 ttttattgcc cgccattttc ctgcgtttga aaagaatgtg aaaacagatt ggcggggcgg
      481 gggaatgccc atgcagcaat tttacgtttt gggttatgag gataaccgtt ataatcacaa
      541 ctttacagtc tgcacagaga aaaccgatgc cttggaatct ccccatcttc ctcacatggt
      601 tgagggtctt gctcattcct gtcctgacag ccctttttta cctgcctttt ccgtggttcg
      661 cggaggaaac gattaatctc accgccgccg tcatttttgc cgttgccgcc ttgaccgact
      721 ggtttgacgg atttttggca aggttgtgga aacagacctc ggatttcggc gccttcctcg
      781 atcccgttgc cgacaagctg atggtcgccg tctctttgct tttactggtc aaactcgacc
      841 ggacctatgt tttgtttgcc atgatcatca tcggcaggga aattaccatt tccgcattgc
      901 gcgaatggat ggcgcaaatg ggcaaaagga acagcgttgc cgtcgccacc gtcggtaagt
      961 ttaaaaccgc cgcgcaaatg ctggcaattt tcctgctgct gctgaatatt cccgattttt
     1021 acggatttaa tcttgttgtt atcggcaaca tattgatgtt tatcgcatct ttgctgacag
     1081 tctggtcgat gctgtattat ctgaaaatgg cgtggaaaga aatcgcctga aaaaaacata
     1141 aaaatagctt gacggcaaaa acagaatcca taataattgc gtctcttcga tgttggagaa
     1201 tgaaatcggg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1261 agttcgagac tcgtttcccg ctccaaaaat ttcattttcc cacaatcatt gcgggaatag
     1321 ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc gagttcgaga ctcgtttccc
     1381 gctccaagtt ttatttcaaa catatcaacg cgggaatagc tcagttggta gagcgcaacc
     1441 ttgccaaggt tgaggtcgcg agttcgagac tcgttttccg ctccaaaaac cgatatgttt
     1501 gaacgcgcgg gaatagctca gttggtagag cgcaaccttg ccaaggttga ggtcgcgagt
     1561 tcgagactcg tttcccgctc caagtttttt agataagcca gttttagggc gagatagcaa
     1621 agtggttatg cagcggattg caaatccgtc tacgccggtt cgattccgac tctcgcctcc
     1681 attatcgtac tatggcgggg tggcagagtg tttatgctat gaggagtgca atcttcatat
     1741 aggccggtta aaatccgcgc ccccgcctcc acctttcaca aatgcccggg tggtgaaata
     1801 ggtagacaca acggacttaa aatccgtcgg gactaaacat cccgtgccgg ttcgattccg
     1861 gctccgggca ccaagctgaa aatgacaata ccgctccaag cggttatttt tttatctgtc
     1921 ggacggtgat ggacaatatt gacatgttca tgcctgaaca agaggaaatc caatcaatgt
     1981 ggaaagaaat tttactgaat tacggtattt tcctgctcga actgcttacc gtgttcggcg
     2041 caattgcgct gattgtgttg gctatcgtac agagtaagaa acagtcggaa agcggcagtg
     2101 tcgtactgac ggatttttcg gaaaattata aaaaacagcg gcaatcgttt gaggcattct
     2161 ttttaagcgg ggaagaggca aaacatcagg aaaaagagga aaagaaaaag gaaaaggcgg
     2221 aagccaaagc agagaaaaag cgtttgaagg agggtgggga gaaatctgcc gaaacgcaaa
     2281 aatcccgcct ttttgtgttg gattttgacg gcgatttgta tgcacacgcc gtagaatcct
     2341 tgcgtcatga gattacggcg gtgcttttga ttgccaagcc tgaagatgag gttctgctta
     2401 gattggaaag tccgggcggc gtggttcacg gttacggttt ggcggcttca cagcttaggc
     2461 gtttgcgcga acgcaatatt ccgctgaccg tcgccgtcga taaggtggca gcgagcggtg
     2521 gttatatgat ggcgtgtgtg gcggataaaa ttgtttccgc tccgtttgcg attgtcggtt
     2581 cggtgggtgt ggtggcggaa gtaccgaata tccaccgcct gttgaaaaaa catgatattg
     2641 atgtggatgt gatgacggcg ggcgaattta agcgcacggt tacttttatg ggtgaaaata
     2701 cggaaaaggg caaacagaaa ttccgacagg aactggagga aacgcatcag ttgttcaagc
     2761 agtttgtcag cgagaaccgc cctcaattgg atattgagga agtggcaacg ggcgagcatt
     2821 ggttcggtcg gcaggcgttg gcgttgaact tgattgacga gatttcgacc agtgatgatt
     2881 tgttgttgaa agcgtttgaa aacaaacagg ttatcgaagt gaaatatcag gagaagcaaa
     2941 gcctgatcca gcgcattggt ttgcaggcgg aagcttctgt tgaaaagttg tttgccaaac
     3001 ttgtcaaccg gcgggcggat gtgatgtagt ttgccgaacg gatggaaatg ccgtctgaag
     3061 cgtgttcaga cggcattttt caagttccgg ctttgatgtt ccgttccaat ccccctatcc
     3121 gttgtccgat gtctttcaaa ttggaatatt gttcgaccaa tcgcggcacg tcgtccaaac
     3181 tcatggcaaa catccacaga taagtgccga ccgaatagac atgccccgcg ctgctgtagc
     3241 ctttgagcgt catcatcaca aaagcaaagc cgaacaaaat acccatcgcc gtgccgacgc
     3301 agagatagcc gaaggcttcg cggttggaaa tcagcacacg caggcgcgca agcagtccgt
     3361 aatggcggta cagctgccgc cggtcgcctt ttcggataaa gtggttgtcg cgttccaagc
     3421 tgttcttcag gcggaaatac aggttttcgc tgatggcggc aaaacgtggc aaaagccata
     3481 aaaacaacgc aagtatgccc accgccgaca cgccgaccca aaattccagc accagcagca
     3541 tgatgcacgc gccgaatatg gatacgacgg atgtcgcggc aatcggcagg tgttcttcaa
     3601 aaaagctgac aaactcacgc gacagggcaa cccgcgcagt taccgccgaa tgcgggactt
     3661 gtcgctgccg ctgttccaac acgaccggca cggcgatttc ggtataaatc cgcgtaaacg
     3721 tgcgcgtatc ggcaatccgc cgcgccgcac cgaccagcca catcaaaagc acaaccaaag
     3781 cgtacagcaa cgcctgccac acctcccccg caatcacggc attgatcgcc cggccgccaa
     3841 acactggata taataccagc atccaaaggt tttccagtcc gaccagggaa aacgtgccga
     3901 gcagacgctt gcggtgggtt tgggctatgt gtttcaacat tttccacata tttgctccct
     3961 attttttatt ttccgagatt gttttttcca tcacttcagc cagtgcatcc aaatcggcaa
     4021 acagccgggt tgtgcgcttg tcgccgaatg tggcaaatac tttgtcgctg aattcctgcg
     4081 cgctttctgt taaaggtgcg gcatgggctt tgcctttttc ggtcagcgac agcaaccgtt
     4141 cgcgccggtc ctgttcgctt tcctgccatt caatcaaccc ttgtccggca agggttttgc
     4201 atacgcctga aacggtctgt ttgggcaaac tccactcttc gccgatatgc ttttgcgtgc
     4261 ggctgccttc ggttgccagg gtataaagta cggcaaagag gttgtaattc agatcctgct
     4321 gcccgatcca tttgtcgaag acattgcaaa tcaggttgat acgggtgcca agttggtcga
     4381 gttggttcat aattggtctt gatattgact aaatttgtgc g
//
LOCUS       DAXHSJ010000153         4417 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000153 DAXHSJ010000000
VERSION     DAXHSJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.153"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(99..1016)
                     /gene="thrB"
                     /locus_tag="ACFM8U_001778"
     CDS             complement(99..1016)
                     /gene="thrB"
                     /locus_tag="ACFM8U_001778"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC6334969.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     ADAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACQGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLG"
     gene            complement(1052..1780)
                     /gene="ubiG"
                     /locus_tag="ACFM8U_001779"
     CDS             complement(1052..1780)
                     /gene="ubiG"
                     /locus_tag="ACFM8U_001779"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC6334970.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIVPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1932..3173
                     /locus_tag="ACFM8U_001780"
     CDS             1932..3173
                     /locus_tag="ACFM8U_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC6334971.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3181..3312
                     /locus_tag="ACFM8U_001781"
     CDS             3181..3312
                     /locus_tag="ACFM8U_001781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334972.1"
                     /translation="MPSEARRRLQTALPQQTATVFRHTAHYPAPHAQIPPRQTGTQP"
     gene            3323..4021
                     /locus_tag="ACFM8U_001782"
     CDS             3323..4021
                     /locus_tag="ACFM8U_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6334973.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQHRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMRRELVGLYGVPPERIQ
                     VAPPPQIRNASSRNPEKLPTCAPNTALPTMKPFSCSHRPATRAKVWNCLPTFSNIPAC
                     PSSSPLSAPRSPAL"
     gene            3904..4377
                     /locus_tag="ACFM8U_001783"
     CDS             3904..4377
                     /locus_tag="ACFM8U_001783"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6334974.1"
                     /translation="MFPSTGHTRKGLELLADFFEHTGLPVKLAVVGSPLPRPMKNVVG
                     LGFCTDMPELYRAADFTIMASLYEPFGLVGVESVLCGTRVVLSENMACTEVMNEEAGF
                     FFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYNPSLSHHIDRLTDILASV"
BASE COUNT         1035 a         1434 c         1024 g          924 t
ORIGIN      
        1 gaaaatactc ggctatcgtc tcattttcgt tttaatcagc cataaaaatg ccgtctgaaa
       61 ccctttcaga cggcatttct gtcaaacgcc ggacgcactc aacccaaact caacagcagg
      121 ttgcggaacg cgttcgggtc tttgataaac gtcatctcgc ccgcctgcgg aaaatgaaag
      181 tccaacaggc gcgacaccca aaaacggatg cagccggcac gttgggcggt cgggaaatac
      241 gccttttctt cggcactcaa ggggcgcacg ccctcataac caccgataaa cgcctttttc
      301 aacgcctcat ccaacttatt gtccgccgtc cttgcccaat cgttgaccgc aatcgccaag
      361 tcatacataa aattgccctg gcaggcataa taaaaatcga tgaagcccga tacctgaccg
      421 ccgtcaagca acacattgtc tttaaacagg tcggcgtgga tgatgcccga aggcagatga
      481 ttgccgagat tgtccttcag cgcatcgatt tcggaacaca gcagcgcggc atcggcttgc
      541 gacaggacag gcagcagccg ggcgcacgcc tccgtccacc acgcatcgta acgcgggttt
      601 tccatttcca aagggaaatc ggcggcggca aggtgcattt tcgccaacat cgcaccggta
      661 tggaaacact gctcagccgt cggcagcgcg gtatccgaac ctttcaggca ggcaactagg
      721 caggcgggct tgcccgccaa aacggaatca agccggccgt ctttgcgcgc aaccggcgcg
      781 gcaaccgcca cgcccttcat actcaaatgc cggttaagct ccagaaaaaa cggcagctct
      841 tcctgtttca acacttcaaa cacggtcagc acataacgtc ccgaagtcgt cgtcagaaaa
      901 taattgctgt tggtaatccc ctgcgcgatg ccctgcaggg aaacaaattc ccccaaatcg
      961 taaccgctca ggaagccgcg catttcatca tcggaaacac tggtataaac agacatatcc
     1021 attcacccgt tcaaaacaat caaacccgcc gtcagaacgc cggacggcag gcaaacatat
     1081 aattcacatc ggtcgaatcg cacagggcat aagtttgcga caaaacatgg taagtcatac
     1141 ccttcgtatc cgccacatcc aagcccgcct gacggcacat ccgcgccagc tcggcaggca
     1201 cgataaattt tttccagtcg tgcgtgcctt tggggacaaa cttcaacaga tattccgccg
     1261 ccacaatcag atgcaggtac gatttcgggt ttttattgat ggtggaaaaa aacaccatgc
     1321 cgtccggttt gaccagcttg gcacaagcac gcacgatggc ggcgggatcg gggacgtgtt
     1381 ccatcatttc catgcacgtt accacatcga acgagtgcgg ttccgcctcg gcaaggtctt
     1441 ccacgcggat acattcgtat tcgatatcgg cgacattgtt caaagccgcg tgcaggcggg
     1501 cggtttccag cgactgctcc gccatgtcga tgcccttgac aaacgccgcg ccgcgccgcg
     1561 ccatgctttc cgccaagatg ccgcctccgc agcccacgtc caaaacccgt ttgccgcaca
     1621 aatccgcgtg tccgtcgata taatcgagcc ggagcggatt gatgtcgtgc aaggttttga
     1681 actcgcccga cttgtcccac catttgtcgg caatccggct gaatttggcg atttccccct
     1741 catcgacatt atattttttg tcggacattt tccctcccat ctgacgaacc gcccactcca
     1801 aaacccaaga ttatactatg gaacacaatg ccgtctgaaa gccttttcgg gcggcgcggc
     1861 gcaaatgcct tacaaatcct tacactttac ggcataatgg cggctcgctt tttctggcag
     1921 aaagacaaaa tatgcccacc aaaacccctt cactgttcgg cggcgcgatg attatcgccg
     1981 gcacggtcat cggcgcaggt atgctcgcca acccgaccgc cacatccggc gtatggttta
     2041 ccggctcgct ggccgtgttg ctgtacacct ggttttctat gctttccagc ggcctgatga
     2101 ttttggaagt caacacccat tatccgcacg gcgcaagttt cgacacgatg gtcaaagacc
     2161 tgctcggacg cggctggaac atcatcaacg gcatcgccgt cgccttcgtt ttatacctgc
     2221 tcacctacgc ctacatcttc gttggcggcg acctgaccgc caaaggctta ggcagcgcct
     2281 tgggcggcaa tgtttcactc accgtcggac aactcgtctt cttcggcatc ctcgcctttt
     2341 gcgtatgggc atccgcacgc ttggtcgacc gcttcaccag cgtcctcatc ggcggcatgg
     2401 tattaacctt tatttgggca accggcggcc tgattgccga tgccaaaatg cccgtcctgt
     2461 tcgacaccca agcccccgtc ggcaccaact actggattta cgccgccacc gccctgcccg
     2521 tctgccttgc gtcattcggt ttccacggca acgtctccag cctgctcaaa tactttaaag
     2581 gcgacgcgcc caaagtggct aaatccatct ggacgggcac actgattgcg ctggtaattt
     2641 acgtcctctg gcaaaccgcc atccaaggca acctgccgcg caacgagttc gcccccgtga
     2701 ttgccgccga agggcaagtc tccgtcctca tcgaaaccct gtccaaattc gcccaaaccg
     2761 gcaatatgga taaaatattg tccctgtttt cctatatggc gatcgccacc tcgtttttag
     2821 gcgtaacctt aggtctgttc gactacatcg ccgacatctt caaatggaac gacagcgtgt
     2881 ccggccgcac caaaaccgcc gcgctgacct tcctgccgcc tctaatttcc tgcctgctct
     2941 tccccaccgg ctttgttacc gccatcggct acgtcggcct ggcggcaacc gtctggacag
     3001 gcatcatccc cgccatgctg ctctaccgtt cgcgcaaaaa attcggcgca ggcaaaacct
     3061 ataaagttta cggcggcttg tggctgatgg tttgggtctt ccttttcggc atcgtcaaca
     3121 tcgccgcaca ggtattgagc caaatggaac tcgtccccgt atttaaagga taaaggcaaa
     3181 atgccgtctg aagcccgccg gcggcttcag acggcattgc cgcaacaaac ggcaaccgta
     3241 ttccggcaca cagcgcatta ccctgcccct cacgcacaaa tcccgccccg acaaaccggg
     3301 acgcaaccat aaaggaacaa tgatgaagct caaaatagac attgcaacca acaacttcaa
     3361 acacggcggc ggcacggaac gctacacatt ggatttggta aagggtctga acagacaaaa
     3421 catcacaccg gccgtttatg cgacgaaatt tgatcacggc attcctgaat acgccatgat
     3481 cgaaccccat cttgtcgatc aacaccggac gctgaaaaaa ctacgctcat tcctcttttc
     3541 aagccggctc gctcaaacca gaaaaaacag tgccgccaaa ctgattgcct gccaccacgc
     3601 cgattacgcc gacctcctca tctgcggcgg cacacacttg ggctacctgc accatatggc
     3661 gcaaaaaccg aacctgctcg accgcctcgc catacgccgt aaccgcagca actacgccac
     3721 cgccaaactg attatggcgc attcccatat gatgcggcgc gaactggtcg gactgtacgg
     3781 cgttccccct gaaagaatcc aagtcgcccc ccccccgcag atacggaacg cttcttcccg
     3841 caacccggag aaactgccga cctgcgcgcc aaatacggct ttgccgacta tgaaaccgtt
     3901 ttcctgttcc catcgaccgg ccacacgcgc aaaggtctgg aactgcttgc cgactttttc
     3961 gaacataccg gcctgcccgt caagctcgcc gttgtcggct ccccgctccc ccgccctatg
     4021 aaaaacgtcg tcgggctggg cttctgcacc gatatgcccg aactctaccg cgccgccgac
     4081 tttaccatta tggcctccct gtacgaaccc ttcgggctgg tcggtgtcga atccgtccta
     4141 tgcggcacac gcgtcgtcct ctccgaaaac atggcatgta cagaggtcat gaacgaagaa
     4201 gccggattct ttttctcacg ccaaaacccg gaaaccctgg cgcaagccgt tgcccaagcc
     4261 gtcagcctta aaaaacaggg cggacaccgc ctgtccgacc cgatgcgtgc actgaactac
     4321 aacccgtccc tgtcgcacca catcgaccga ctgaccgaca tactggcatc cgtataacaa
     4381 aatgccgtct gaacgagttt tcagacggca ttgctgc
//
LOCUS       DAXHSJ010000154         4316 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000154 DAXHSJ010000000
VERSION     DAXHSJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.154"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="ACFM8U_001784"
     CDS             complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="ACFM8U_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC6334975.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKAFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2288..2560)
                     /locus_tag="ACFM8U_001785"
     CDS             complement(2288..2560)
                     /locus_tag="ACFM8U_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334976.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEKFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2746..3432
                     /locus_tag="ACFM8U_001786"
     CDS             2746..3432
                     /locus_tag="ACFM8U_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6334977.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3564..3902
                     /gene="erpA"
                     /locus_tag="ACFM8U_001787"
     CDS             3564..3902
                     /gene="erpA"
                     /locus_tag="ACFM8U_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC6334978.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3919..4065
                     /locus_tag="ACFM8U_001788"
     CDS             3919..4065
                     /locus_tag="ACFM8U_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334979.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4062..>4316
                     /locus_tag="ACFM8U_001789"
     CDS             4062..>4316
                     /locus_tag="ACFM8U_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6334980.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYS"
BASE COUNT          994 a          867 c         1134 g         1321 t
ORIGIN      
        1 aaaaatttcc agagaaccga tacaacagtt ggaacttggg tttgggaata ttacggtaga
       61 tgaacttgga acctctgtta tgctatggtc ttttatctca attgaaaaaa gcgcgaatca
      121 aacggttcgc gcttttttca gacggcatca attatttttt gtcgtctttt acttcttcaa
      181 agtcggcatc tacgacatca tcgtctttct ttgcagaagc attggcttgt tcgctttcgc
      241 ctgcttgggc ttcagcttgt gcttgagcgt aaaccatttc gcccagtttt tggctggctg
      301 tgcccagcgc ctcggctttg gcatcgatgg cggctttgtc gtcgcctttc acggcttctt
      361 cggcttcttt cagcgcggct tcgatttttt ctttttcggc agcgtcgagt ttgtcgccgt
      421 agtccgccaa agattttttc acggagtgaa tcagggcttc ggcttggttg cgggaagcga
      481 ccaattcagt cagcttttta tcttcctcag cattggcttc ggcatctttc accatgcgtt
      541 cgatttcttc ttcgctcaaa cctgaagaac cttggatggt aatgttggcc gctttgccag
      601 tgcctttgtc tttggcggaa acgtgcaaga taccattggc gtcgatgtcg aaagtaactt
      661 caatttgcgg cataccgcgt ggtgcaggtg cgatgtcgcc caagttgaac tgacccaaag
      721 atttgttggc agaagcgcgt tcgcgttcgc cttgcagtac gtggatggtt actgcgcttt
      781 ggttgtcttc ggcggtagag aacacttgcg acgctttagt cggaatagtt gtgttctttt
      841 gaatcagctt ggtcatcacg ccgcccatgg tttcgatacc caaggacaga ggggttacgt
      901 ccagcagcaa tacgtcgctg cggccgccgc ttaacacttc gccttgaatc gctgcgccta
      961 cggcaacggc ttcgtcgggg ttcacgtctt tgcgaggctc tttaccgaag aaggctttaa
     1021 cggcttcttg tactttcggc atacggcttt gaccaccgac caagattacg tcgtcgatgt
     1081 cgccggtgct cagaccggca tctttcaatg cggtgcggca aggctcgata gagcgggcaa
     1141 tcaggtcttc aaccaggctt tcgaatttgg cgcgggtaat tttcatcgcc aagtgtttcg
     1201 gaccggttgc gtccatggta atgtacggca ggttgatttc ggtttgctgg ccgctggaca
     1261 attcgatttt ggctttttcg gcagcttctt tcagacgttg cagagccatt acgtcttgtt
     1321 tcaaatcaat gccttgttct tttttaaact cgtcaatgat gtagtcgatc aagcgttggt
     1381 cgaagtcttc accgcccaag aaagtatcgc cgttggtagc caatacttca aattgtttgt
     1441 cgccgtcgag gttggcgatt tcgatgatgg aaatatcgaa agtaccgccg cccaagtcat
     1501 atacggctac tttgcggtct ttgttgtcgc ctttgtccat accgaatgcc aaagcggctg
     1561 cggtcggctc gttgatgatg cgtttcacgt ccaaaccggc gatacggcct gcgtctttgg
     1621 tagcttgacg ttggctgtcg ttgaagtagg cagggacggt aatcacggct tcggttactt
     1681 tttcgcccaa gtaagcttcg gcggcttctt tcattttacg caggacttct gcggaaattt
     1741 gaggaggaga cagctctttg ccttgtgctt ttacccatgc gtcgccgttg ttggctttga
     1801 tgatttcgaa aggcatagat tcgatgtcgc gttggacttc tttgtcttca aatttgtggc
     1861 cgatcaggcg tttggcggcg taaatggtgt ttttagcgtt ggttacggct tggcgtttgg
     1921 caggcgcacc gacgaggatt tcgccgccgt ccaaataagc gataacggac ggcgtggtgc
     1981 gtgcgccttc tgcgttttcg atcactttgg tttgaccgtt ttcggaaatg gccaaacaag
     2041 agttggttgt acctaagtcg ataccgatta cttttgccat gtggataatc ctatttgatt
     2101 ttgcttattt tgagaaatat gttggaacat tttgtcccga tgggctgtaa atagggcggg
     2161 cggcgggctg tttcaagcta cagcatggct ataagtatat aactttatga atatattggt
     2221 tttatatttg atttaataca tttggctcca atgcatttaa gcataatatt tcaaatggca
     2281 ggcaggttta ttcatagacg atgccggcga gcatttcctg ttcgttcaag ttgccgtact
     2341 ctttttccca gtcgtgtgaa gactcgatga tgtcgcattc tttggaaagg gagacttgtt
     2401 ctgcatccat atctttggcg ttcagtatgt tgaatttttc gcacagggat gcggataaag
     2461 tgatgtcagg ctgtttggct tcagaacggt tttcttggaa ggcaaagcag aatgcggtaa
     2521 atgccgcagc atagataaga tatttgccgg ttttcttcat tttctatcct ttttctgtca
     2581 actcaggatt aaacctatgg aaaatcttaa aaattatgta ttgagtaaga aaaatcataa
     2641 tttaaattta gtttatcata gttgttccgt ttttgggata gctaaggtaa aatatatttc
     2701 atgtttactt tagatgattg aatgaagggg agcggaagga tatttatgga aacctttaaa
     2761 gacagactgg tttttttatg gaaaagcgaa gcgaagcagg caaaaatcgc atccgatatt
     2821 gaaatgacga ttgcgggctt cagcaggata tggaatgaag gcggtctgcc gaagtctgaa
     2881 acattaaaaa aaatcaagca gttgaagggg tgtagtatcg attggctgct gaccggggag
     2941 ggtaatccgt ttccggatga agccccaaaa aaatcccttg cttacgatac cttgggcaat
     3001 gaagttgata cggacgagtt tgtctttgtg ccgagatatg atattcgggc ggctgcggga
     3061 tacgggcagt ttgtcgatca tgaggaaccg gtatttacaa tggcgttcag acggcattgg
     3121 attgagaatt atgttacccg cgatacgaaa aacctgtctg taatttccgt caagggggat
     3181 tctatggagg gggttttgaa tgacggcgat tcgattttgg tcaatcatgg tgaaaatacg
     3241 ccgagggacg gtctgtatgt gttgcggatt aatgaaaatc tgctggttaa acgtttacag
     3301 attgtaccgg gcgggattat caatgtgatt tctgcaaacg aggcttatcc tgcttttgaa
     3361 atcaatttga acgatttgac cgatgatgtg gagattatcg ggcgtgtcga gtggttcggc
     3421 aggacgattt gagtttgggg cttgaaattg caggcggtca aacttatcta ttggaacaat
     3481 tcctttttca aaggcgaagc ctgcttgcct ttaaaggggg tttgagagag aatgcagaaa
     3541 atattatatt aaggaatcac accatgtcgg atgaaagccc tattattttt actgacagct
     3601 gctgtgccaa agttgccgat ttgattgccg aagaaaacaa tcccgatttg aaattgcggg
     3661 tttttgtcaa tggcggcggc tgttcgggtt tccagtacgg atttactttt gacgaaatca
     3721 aaaacgacga cgattttgaa attgagaaaa acggtttggt ctttttggtc gatccgatga
     3781 gctatcaata tctggtcggt gcggaaatcg actatacgga aagtttgcag ggttcgcaat
     3841 tcgtcatccg caatccgaat gcggaaacaa cctgcggttg cggatcgtcg ttttccgtat
     3901 gaccgcttgg tttgtgtgat gccgtctgaa cgttcagacg gcatttttac ttttggaaaa
     3961 tatattatcg ggatgaattc acatataatc cgattgtttg aagatgaatc gggtttcccg
     4021 aaaggaacgg gcggaacggt atcaggcgta tttgttccct tatgattgag atgagtaaag
     4081 attaccgaaa tgatttgtac gatgtatatg tttcctaccc gccccaagtg gatcgcgggc
     4141 ttatccggga gtgcctcagg gagaatctcg gcgaggaaaa ggcggaagga ttgatcgaat
     4201 cgctcgattc caaacctcaa gtgctggttg aggaaaaatg cacttgggcg aaacgggaag
     4261 agttgcacga ttatttcagc tatttgggtt tggatattat tacccggaga tatagt
//
LOCUS       DAXHSJ010000155         4314 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000155 DAXHSJ010000000
VERSION     DAXHSJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.155"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(47..1303)
                     /locus_tag="ACFM8U_001790"
     CDS             complement(47..1303)
                     /locus_tag="ACFM8U_001790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC6334981.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTSISQIKDLLDEEETAFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQPAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDTRITRLAGKADHVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSVLPEENELQVERFLSEHPEFEPVNCAELLQSLKVDL
                     DTGKYLRLDSAKHQTDGFFAAVLQRK"
     gene            complement(1338..2192)
                     /locus_tag="ACFM8U_001791"
     CDS             complement(1338..2192)
                     /locus_tag="ACFM8U_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC6334982.1"
                     /translation="MNYALDALWWKLTDPTVRDLASLLTAPPLWQSGCELGVRELLGK
                     RGFRYLLALDADPAPLTEHLARHAPFGHRLGIYAEELSAFWFANAPHAELLAHNLTVS
                     GSDGSTQGAADFVARLNGKPCHIELTCKYYGGTGKPEDMRGLDPKDTLLGKAAKLTAQ
                     LGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPQGFNAFEPPLNPYGWRGIYIQDW
                     AEYGFERREARYHLLDRMAYLAPARVAETETLNETEIRRIDNGLIAVLECRPDGFWHE
                     IERIMKAV"
     gene            complement(2263..3081)
                     /locus_tag="ACFM8U_001792"
     CDS             complement(2263..3081)
                     /locus_tag="ACFM8U_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC6334983.1"
                     /translation="MTAPKPELPDYLGNIRIVLTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFNPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTLQQRLKDGQS"
     gene            3347..4132
                     /locus_tag="ACFM8U_001793"
     CDS             3347..4132
                     /locus_tag="ACFM8U_001793"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6334984.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDVVAANPKVLAQMLKIISAHV"
     gene            complement(4113..>4314)
                     /locus_tag="ACFM8U_001794"
     CDS             complement(4113..>4314)
                     /locus_tag="ACFM8U_001794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6334985.1"
                     /translation="QIVQGSPFNQIWVSIFGSFGHLAVSTFLPPLPVKMPSEISARYF
                     RRHTRPFSRIPGSGLKFKRVRK"
BASE COUNT          881 a         1069 c         1308 g         1056 t
ORIGIN      
        1 atgaaatgcc gtctgaacgc tttgaaaagg gttcagacgg ccagggttat ttacgttgca
       61 atacggcggc gaagaagccg tcggtttggt gctttgccga atcgaggcgc aggtatttgc
      121 cagtatccaa atcgaccttc aggctttgca gcagttcggc gcagttgacg ggttcaaatt
      181 cgggatgttc ggacaggaag cgttcgactt gcagttcgtt ttcttccggc aacacgctgc
      241 aagtggcgta aaccaagcgg ccttgaggtt ttaccagttt ggaggcggca tcgaggatgc
      301 tgtgttgctg ttccaaaagg ttggcgacgg tctcagcaga ttggcggtat ttgaggtcgg
      361 gattgcggcg cagtgtgcct aagccggagc agggcgcatc cactaaaacg tggtcggctt
      421 tgcctgccag tcgggtgata cgggtatcat gttcgctgct gatgcgttcg gggtggatat
      481 tggtcagtcc ggcgcgggtc atacgcggtt tgaggttggc aaggcgtttt tcggcgatgt
      541 cgaaggcgta gattctgcct ttattggcca tttgcgcacc gacagccaag gttttgccgc
      601 cggcaccggc acagaaatcg acaatgattt cgccgcgttt tgcgcccacc aataaagcaa
      661 gcagctggct gccttcgtct tggacttcca gtgtgccgtc taaaaacagt tcgtgtttgt
      721 taagcgcgat tttgtttttc aggcggatgc cccaaggcga ataaggcgtg gcctctgcat
      781 cggcactttc ggcttgcaac agcggcagca ctttatcgcg tttgcctttc aaagtgttga
      841 cgcggatgtc gagcggggca ggctggttga tgctgcggcc gaaggcgagg atttcttctt
      901 cgcgccaatg ctgttgcagc tgttccacca gccattgcgg caattctgcg gcggtattca
      961 ggccgtctga aaactcggtt ttacgggctt tcaaattgcc gaggaacgct gtttcttctt
     1021 catcaagcag gtctttgatt tggctgatgc tggtgcttct gccgagaacc agtgcggcga
     1081 gtgcggcttt gcgtggctgc gcgtgcggac ggcgcagggc ggtactgatt ttttgatagt
     1141 ggcgcagcgc ggcaaaggcg gtttcggcga tttcgtggcg gtcttggcgg ccgagttttt
     1201 tgtgttcgcg gaaataggcg gagaggacgg catcggcagg ctgtttgaaa gtcagcattt
     1261 cagccaaaac tttggcggta tggtcgagtt gtgcggcgtt catcattaat gggttctctt
     1321 aaagttagta aatggtttca gacggccttc ataatgcgtt cgatttcgtg ccaaaaaccg
     1381 tccggccgac attccaaaac ggcaatcaaa ccattgtcga tgcggcggat ttcggtttcg
     1441 ttcaatgttt cggtttcggc aacacgcgca ggcgcgagat aggccatgcg gtcgagcagg
     1501 tgatagcgcg cttctcggcg ttcaaacccg tattccgccc aatcttgaat atagatgccg
     1561 cgccagccgt atgggttaag cggcggctca aaggcgttga aaccttgcgg aaaaaatccg
     1621 atgccgcgca cgatggaaac gggttttacg tcaagcggca gaccgtgttg ccgcaaggtt
     1681 cggatgccgt ctgaagtgtg cggcagaccg agttgggcgg tcagtttggc ggcttttccc
     1741 aacagcgtgt ctttggggtc gagtccgcgc atgtcttcgg gtttgcccgt gccgccgtaa
     1801 tatttgcagg tcagttcgat atggcagggc ttgccgttaa gtcttgccac aaaatccgcc
     1861 gcgccttgcg tgctgccgtc cgaaccggaa accgtgaggt tgtgcgcaag cagttcggcg
     1921 tgcggcgcgt tggcaaacca aaatgccgac agttcttcgg cgtaaatgcc gaggcggtgt
     1981 ccgaatgggg cgtgtcgggc gaggtgttcc gtcagcggcg cgggatcggc atccaatgcc
     2041 aaaagatagc ggaaaccgcg tttccccaat agttcgcgca cgcccaattc gcaaccgctt
     2101 tgccacaaag gcggcgcagt cagcagcgag gctaaatccc gcacggtagg gtcggtcagt
     2161 ttccaccaca gggcatcgag ggcgtagttc atggttttcc tgtttctgta ttctgatttt
     2221 tcctgaaatg ccgtctgaaa aacggattca gacggcatgc gcttaggatt gcccgtcctt
     2281 cagacgctgt tgcagcgtat tgaaaaaacc gcgcaggatg tcgatgtctt cggtttgcgt
     2341 gttggcgcgt ccgaacaggc tctgcatacg gcgcatcagg cgctcgccgt tgcggcggtt
     2401 gaaaaagccg atgtcgttca tcacgctttc catgtgggcg accatgcctt tgatttgctc
     2461 gtgggtcgca gcgtggtctt cttgttgaag atgggtcatg ggcgaatcgg tttgactgaa
     2521 gatttcgtag cacacgacct gcacggcttg ggcgaggttg agcgagaaat agtcgggatt
     2581 gccgttgatg gtcatcagtc ggttgcaggc ttggacttct tcgatgctca agccgaaagt
     2641 ctcgttgccg aaaaccagtg ccactttctc gcctcggttg gcggcctgca gtaattcggg
     2701 caccaaatcg cgcggggttt gcagcggcgc ggtaatttcg cggcggcggc tggtcagggc
     2761 gcaggcgatg gtggtgtcgg caagggcttc gtccaaagag gcggcaatgg tggcattttc
     2821 caaaacatct gccgcaccgg aagcgaggat gaagctttct tccggtaatt taaacgattg
     2881 cggatgctcc ggattgaaca cgggcggatt ctccgtcatc ggcgtagcca tcagattggg
     2941 ggcgacgatg gtcagtttgt gcaggcccat tgttttcatc gcgcgcgcgg cagagccgat
     3001 attggcggga tggctggtgc gggtcaggac gatgcggatg ttgccgagat agtcgggcaa
     3061 ttcgggttta ggggcagtca tgttcggttt tcggtcggaa tggaataggg tataatgcgc
     3121 cgtctttttc gttcggacgg tcaaatgccg tctgaaatgt tctttaacaa cgtctgaaat
     3181 gttctttaac aacgtcggaa atttaacgtt gcgcctgaag cagacacgcg tcaggcggta
     3241 ttttacccga tttggcagga tttaccccaa ccaaataaac tccgattttg ccgctccgaa
     3301 ggactgtacg tccgaaccgg cggtatgcca cccttaagga aatctgatga atccgttttt
     3361 gaatacagcc tttaaagccg cccgccgtgc cggtcagatg atgatacgcg ccgcgggcaa
     3421 tctcgatgcc gtcaaaaccg acagcaaagc cttcaacgat tttgtttccg atgttgaccg
     3481 caattccgaa atcatcctgg ttgaggcttt gaaagaagcc tatccgcacc acaaaatcac
     3541 ttgtgaagaa agcggctccc acggcaaagc cgcagccgag tacgaatgga ttatcgatcc
     3601 gctcgacggc acgaccaatt tccttcacgg tcatccccaa tacgccatct ctatggcgct
     3661 gctgcacaaa ggcgtgttgc aagaagcttt ggtttacgcc cccgaacgca acgacgtata
     3721 tatggcttcg cgtggcaaag gcgcgttgct caacgaccgc cgcatccgcg tttccaaccg
     3781 catcgaactg aaccgctgcc tgattggtac cggcttccct gttgtcgatc aaagcatgat
     3841 ggacaagtat ctggcgattt tgaaagattt cttggccaaa accgccggcg gccgtcgtga
     3901 aggtgcggct tctttggatt tgtgcgccgt agcaacagga cgttttgacg gctttttcga
     3961 gtttaacctc aaaccgtggg acattgccgc cggtgcattg attgttcagg aagcgggcgg
     4021 tatcgtaacc gatatgtcgg gtgaagacgg ctggctggaa agcggcgatg ttgtcgctgc
     4081 caatcccaaa gtgctggcgc aaatgttgaa aatcatttcc gcacacgttt aaatttcagc
     4141 ccgctcccgg gaatgcggga aaacgggcgg gtatgccgtc tgaagtatcg cgccgatatt
     4201 tcagacggca tttttactgg cagcgggggc aggaaggtgg aaacggctaa atgcccgaaa
     4261 ctgccgaaga tggaaaccca tatctgatta aacggagaac cctgtacgat ttgt
//
LOCUS       DAXHSJ010000156         4262 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000156 DAXHSJ010000000
VERSION     DAXHSJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.156"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            539..2266
                     /gene="ilvB"
                     /locus_tag="ACFM8U_001795"
     CDS             539..2266
                     /gene="ilvB"
                     /locus_tag="ACFM8U_001795"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC6334986.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQEFMPSEDALGKWWKTIEEWRSQDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            2277..2768
                     /gene="ilvN"
                     /locus_tag="ACFM8U_001796"
     CDS             2277..2768
                     /gene="ilvN"
                     /locus_tag="ACFM8U_001796"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC6334987.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2835..3128
                     /locus_tag="ACFM8U_001797"
     CDS             2835..3128
                     /locus_tag="ACFM8U_001797"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC6334988.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3208..4221
                     /gene="ilvC"
                     /locus_tag="ACFM8U_001798"
     CDS             3208..4221
                     /gene="ilvC"
                     /locus_tag="ACFM8U_001798"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC6334989.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1114 a         1011 c         1073 g         1064 t
ORIGIN      
        1 aatcaatata ttaagtcgtt gttagccctc ttggttctgt caatgccgtc caatacagtc
       61 cggtgcaatg agccattttc aggaagtatg gttgggaata tttttgatat atcaaaaata
      121 aaatcaagtt caatcaatat cccgagtctt tattgggatg aaaccctgta aattttaaaa
      181 ttcttgatat cgaagaaggg tttttcacat ctgcccctcc tacacaaact gtcaacaatt
      241 tccatctttc aaaaaaggta ctcctgattt tattctttta aaatcaatca ataatttctc
      301 tgctttcaaa aaatattaaa agatgaaaga tttttcttga ttgaactgtc ggtttgcgtt
      361 atcgtttcca ctctttttca caaactctgt gttcctttcc aattgattgg ataatccgtc
      421 tgttgccgtg ttcccccaat gcgcgtaccc taaatcgctg cttggtggaa ttgcattaca
      481 ggtgctgtgg caaagcggtt tgtccctatt ggtttgaaac cgtataaaag aggtcgttat
      541 gcagttatca ggcgcgcaaa tcatagtgca gagtctcaaa gccgaaggtg tcgagtacgt
      601 tttcggttat cccggcggtg cggttatcga aatctacgat gcccttttcc aactcaataa
      661 attcaagcac attctgaccc gtcacgagca ggcggcagta cacgcggcag atgcgtatgc
      721 gcgcgtcagc ggtaaggtgg gcgtggcatt ggttacatcc ggcccgggcg ttaccaatgc
      781 actgaccggt attgctactg cctatacgga ttcgattccg atggtggtca tcagcgggca
      841 ggtaggcaat tccctgattg gtacggatgc gttccaagaa gttgatacgg tgggtattac
      901 ccgtccgtgc gtcaaacaca atttcctggt tacggacatc aatgagttgg cggaaaccat
      961 taaaaaggcg ttccaaattg ccgcaagcgg ccgaccgggg cccgtggtgg ttgatgtccc
     1021 gaaagatgtt acgcaggcga tggcgaaatt cagctatcct caggaagaca tttttatccg
     1081 ttcgtatcaa ccggttgttc aagggcatat cggtcagatt aaaaaggccg tgcagatgtt
     1141 ggcatctgcc aaacgtccgg tcgtctattt tggcggcggc gtggtgttgg gtaatgcttc
     1201 tgaagagctg acccgatttg tccgaatgac gggtgctccg tgcacgggta cgctgatggg
     1261 cttgggcgct tatccttccg gcgaccgcca attcctcggt atgctcggta tgcacggtac
     1321 ttacgaggca aaccttgcca tgcagaatgc ggatgtcgtt cttgccgtag gtgcgcgttt
     1381 tgacgaccgt gtggtttccg taccgtccaa atttttcgag aaggcgaaga aggttatcca
     1441 tatcgatgtc gatccttcca gcatcgccaa acgcgtgaag gtggacattc cgattgtcgg
     1501 cgacgtgaaa aatgttttgt ctgagatggt tgcgctgtgg caaaaacaag agttcatgcc
     1561 gtctgaagat gctttgggca aatggtggaa aaccatagag gaatggcgtt cccaagattg
     1621 cttgtggttt gacaacggca gcgaaattat caagccacaa tatgtgattc agaagcttgc
     1681 cgagattacc ggcaattcgg caatcatcac atcggatgta gggcagcatc aaatgtttgc
     1741 ggctcaatat tatcccttcg aacgtccgcg ccaatggctc aattccggcg gtttgggtac
     1801 gatgggcgtg ggtctgcctt atgcgattgg tgcaaaactt gccgccccgg atcaagacgt
     1861 attctgtatt accggcgacg gctcgattca gatgaacatc caagagttgt ccacctgttt
     1921 ccaatatcgg attccggtta acgtcattac gctgaacaac ggttatctcg gtatggtacg
     1981 ccagtggcag gaaatatatt acggcggccg agagtcggaa acttatttcg attctttgcc
     2041 cgatttcgtc aaacttgccg aggcatacgg ccatatcggt atccgcgtgg acaagaagtc
     2101 tgatgtggaa ggtgcgttgt tggaagcatt gaaccaaaaa gacaggctgg tgtttatcga
     2161 cttcctgacc gaccagaaac agaatgtgat gcccatggtc ggcaacggca aaggtttgga
     2221 cgaaatggta cttccgccgc atatgcgtgc ggacgaaaag gcgtaaggag agacaaatgc
     2281 gacatatctt atctgttctg attgaaaacg aatcaggtgc gatgagccgc gtggtcggtt
     2341 tgttctctgc acgcgattac aatatcgatt ctttggcggt tgcgccgacc gaagacaaaa
     2401 ccctgtcccg tatgaccatc gttacccacg gcgacgagca ggttatcgaa caaatcacca
     2461 agcaactcaa taaattgatt gaagtgatta aagtggtcga tttgaacgaa agccgttttg
     2521 tcgaacgaga actgatgttg gtaaaagtcc gtgccgccgg taaagaccgc gacgaatttt
     2581 tgcgtttgac cgaaatctac cggggcagca tcatcgacgt aaccgaccgc agctatacga
     2641 ttgaaattac cggctcgacc gacaagctgg attccttttt ggaaacggtc ggacgcgccc
     2701 aaattttgga aaccgtacgt acaggcgcgg ccggcatcgg tcgcggtgag cgtattttga
     2761 aaatttaacg ccgtaacctt tcagacggca cggtatttga atgccgtctg aaaaacgaac
     2821 ggcaggagag atttatgtcg aacattaaaa ttgtcgcact ggttaccgtc aaaccggaat
     2881 acacagaaac actggcagca cagtttaaag aactggtcaa agccagccgt gcagaagagg
     2941 gcaacatcag ctacgatctc catcaggaaa tcggcaaacc gaaccgtttt gttttcgtgg
     3001 aaaattggaa atctcaagca gctattgacg aacataacgc cagcgcgcat ttccaagcct
     3061 tcgtccagtc cgtcgacggc aaaaccgaag cgttggaaat cgtattgatg aatgaagttg
     3121 ccgattaagc ggcacactct gtttaacccg tccgaagccg ctcaacattt taaggcttcg
     3181 acaaccattt accttaaagg aaatcaaatg caagtctatt acgataaaga tgccgatctg
     3241 tccctaatca aaggcaaaac cgttgccatc atcggttacg gttcgcaagg tcatgcccat
     3301 gccgccaacc tgaaagattc gggtgtaaac gtggtgattg gtctgcgcca aggttcttct
     3361 tggaaaaaag ccgaagcagc cggtcatgtc gtcaaaaccg ttgctgaagc gaccaaagaa
     3421 gccgatgtcg ttatgctgct gctgcctgac gaaaccatgc ctgccgtcta tcacgccgaa
     3481 gttacagcca atttgaaaga aggcgcaacg ctggcatttg cacacggctt caacgtgcac
     3541 tacaaccaaa tcgttccgcg tgccgacttg gacgtgatta tggttgcccc caaaggtccg
     3601 ggtcataccg tacgcagtga atacaaacgc ggcggcggcg tgccttctct gattgccgtt
     3661 taccaagaca attccggcaa agccaaagac atcgccctgt cttatgcggc tgccaacggc
     3721 ggcaccaaag gcggtgtgat tgaaaccact ttccgcgaag aaaccgaaac cgatctgttt
     3781 ggcgaacaag ccgtattgtg cggcggcgtg gtcgagttga ttaaagcagg ttttgaaacc
     3841 ctgaccgaag ccggttacgc gcctgaaatg gcttacttcg aatgtctgca cgaaatgaaa
     3901 ctgattgttg acctgatttt cgaaggcggt attgccaata tgaactactc catttccaac
     3961 aatgcggagt acggcgaata cgttaccggc cctgaagtgg tcaatgcttc cagcaaagaa
     4021 gccatgcgca atgccctgaa acgcatccaa accggcgaat acgcaaaaat gtttatccaa
     4081 gagggtaatg tcaactatgc gtctatgact gcccgccgcc gtctgaatgc cgaccaccaa
     4141 gttgaaaaag tcggcgcaca actgcgtgcc atgatgcctt ggattactgc caacaaattg
     4201 gttgaccaag acaaaaactg attgttttca aacgggacta cctacacatc gtgtaggcag
     4261 tt
//
LOCUS       DAXHSJ010000157         4221 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000157 DAXHSJ010000000
VERSION     DAXHSJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.157"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(245..1108)
                     /gene="rpoH"
                     /locus_tag="ACFM8U_001799"
     CDS             complement(245..1108)
                     /gene="rpoH"
                     /locus_tag="ACFM8U_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC6334990.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(1372..2910)
                     /gene="lnt"
                     /locus_tag="ACFM8U_001800"
     CDS             complement(1372..2910)
                     /gene="lnt"
                     /locus_tag="ACFM8U_001800"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC6334991.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(2979..3705)
                     /locus_tag="ACFM8U_001801"
                     /pseudo
     CDS             complement(2979..3705)
                     /locus_tag="ACFM8U_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            3899..4219
                     /locus_tag="ACFM8U_001802"
     CDS             3899..4219
                     /locus_tag="ACFM8U_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HFC6334992.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSARLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPLAVFGGLWKFNYSLREKAYSRIEMQSDTVLQAGIWRGTSCPT
                     ARSI"
BASE COUNT          907 a          962 c         1312 g         1040 t
ORIGIN      
        1 atttgtactg tctgcggctt cgccgccttg tcctgatttt tgttaatcca ctatatcaat
       61 tccgccaatc tgtcggaaaa gcagctgatg cggcagtgtc tggtgcatgt ctgctttttg
      121 atttcggcga ttgcgatggt ttggacggat aaaatcgtgt acggcacgac gcacaaaccg
      181 cattgatgtt taccaaataa aatacccgac aaaacaattt gtcgggtatt ttattgcgta
      241 tatttcaaac cgcttcggct tcttcggtca ggaaaccacg cagtttctgc atggcttttg
      301 cttcgatttg gcggatgcgc tcggcagaga cgccgtattc tgcagcaagc tggtgcagcg
      361 tcagcccgcc gtcgtcttga agccagcggc tttccacaat acggcggctc ctgtcatcca
      421 gttgcgccaa agcgttttgt aaaccttctg tttgcagggc gtaatgcgcc tgtttcgata
      481 gttgtcggct cggttcggaa tcgtggtcgg caagccagtc gatgggggcg aaactgtcct
      541 cgtcgtcgct gttgtctgcc atgatggcga tgtcgtgtcc cgtcatgcgc tgttccattt
      601 ccagaacttc ggaaagtttg acacccaaat cgtcggcgat gtcttgtgcc tctttgggag
      661 acagggcgtt gaggttttta cgcatactgc gtaaattgaa aaacagcttg cgttgcggtt
      721 tggtggtggc aacgcggaca aggcgccagt tcctcaggat gaattcgtga atttccgctt
      781 taatccaatg cacggcaaat gaaaacaggc gtgcacctcg acctggctcg tagcgtttga
      841 ccgccttcat cagtccgata ttgccttcct gaatcaggtc tgcctgattc agcccgtagc
      901 cgtcatagcc gcgcgcgatg gaaacgacga cgcgcaggtg ggacaggatg agttgtttgg
      961 cggcgttgag gtcgcccttt atccggcgtt cggcgaggcg ggtttcttct tcctgggaca
     1021 gcatcgggat gctgttgacg gtgtggatgt attgttcgag gctgccgttg ccgctttgga
     1081 tggcgggtaa tgcgaaagcg ttattcattt ggggcatttc ctttcggctg aaactgcgta
     1141 tcggcggttt gctgtgttgg gatgcagtat atcactgctt ggcttgtatt ttgtatgttt
     1201 ggcaggagat atgcgctaag gtttgaaaga caggaaaatt ttgtaaggca ggtttgattg
     1261 attttgtaaa cctgatggct caattcgatt tcagaattat attacatacg taattgtatg
     1321 taaatagccg ttttgaaaaa agacagcccg tccggacggg ctgtgcaggt atcagtgttc
     1381 tttgtttcgg aagatgaaaa gaatcagtgc ggctagggtc aatatgccca tcaaccacca
     1441 tgaactgccg gttttcatat agggcgtttc gccgacatag cctttgatgt gtccttccaa
     1501 tacggtttcc gtatcgggtt gggcttgggc gatgatgttg cctttggggg agatgatggc
     1561 ggttgcgccg gtgttggtgg cacggaccat atagcgtccg agttccatag cccgcgcctg
     1621 cgattgttgg aggtgctggt acatggcgtt ggattttccg taccacgcca tattgctggc
     1681 attggcaagc agtgtggcat ctttggcggc ggcaatcagt tcgtcgccga atccgtcttc
     1741 gtaacagatg ttgaaggcga ttttttggtt tttcatcagc agggcggatt gcttaccgcc
     1801 gcctttgcgg aagtcggaaa ggggcatatt catcattttg taaagcggcg tggtcaggaa
     1861 gggcagcggt ttgtattcgc cgaaggggac gaggtggttt ttggcgtagt aggggatgcc
     1921 gtcctgattg ttttcctgat aaccggtcag gttgatgacg gcgttttcgt aaccgttgcc
     1981 gtccgaagtg tattggctga tgccgacggc gagcgcgctg ccgttgtttt gcgcctgttc
     2041 ggcaaatttt gccagtatgt tttccggcag gttttggcgc ataacgggga tggcggtttc
     2101 gggcaggatg acgatgtcgg cagtggtttt gccgacctgt tcgtaatatt tctgtatggt
     2161 cgggatgact tggtcttcac gccatttgag ggtttggtcg atgttgcctt gaagcagggc
     2221 gacggtgctg cggctgccgt cggggcgggt gaagtcggtt tgtcgggcgg tgtagcctgc
     2281 ggcaagcagg gcggcaatca ggataattgg aagcaggcgt ttgcccgaac gtgcggtgtt
     2341 atcactcgcc aaaaccagcc agacaccgag aaaggcggtt gccagtgtaa ccatgtggat
     2401 gccgcccaat ggggcaaagc cggcgagcgg gctgtccggg gtgatttggg agtagccgat
     2461 tgcgccccag ccgaatccgg tcaggaaacg ttcgcgggca aactcggtca gtgtccacag
     2521 gatgggcagt accaaaccga tttttatgcc ccgaggcagg gtaaattttt tccacagcca
     2581 gaaacacagt gccggataaa gggcaaggta ggcggggagt aggaaggtca gcggtacggc
     2641 atagaggtcg ggcaggccgg aaacgtcgtg cagggcggtg tgtatccagt agaactgtgt
     2701 cgtgtatgcg gtcaggccga acaggtaggc ggaagagaca gcaaaacgcg gacgcagttc
     2761 gatgaggcgg acgaaggcac cgaaaatcaa gggcatcagc caaaagtggt agtagggtgc
     2821 gaaggtaaag ggggtggcgg cggcaaaaag gatgagcaaa ggccagtaga gggcggggtg
     2881 ctgccagtat ttgtccagtt tggaaaccat attcatctgt ctgttcggaa gataccgtct
     2941 gaacatcttt caaacggcat cggtatttga aaaagaaatc aatgcctgcc gaaacgattc
     3001 atcagcggca aggcgggggc gcaggcaatc gaaccggaaa gcagcccgac aacgaggttg
     3061 gcgaagtact ccgcccagcc agcgtcccag tgttgcgcgt gcaggaaatc gtgcagaagg
     3121 ccgaggttgt gggcgaccag tacgccgccg ataagaaaca tggcaagcgt gccgaccacg
     3181 ctcaaaccgc gcataaagca aggcataaag gcagtcagca tttgccccaa actgcgcgaa
     3241 aaggtttgtg ggcggcgcat cagcagcatg cctaagtcgt cgagtttgac gatgacggca
     3301 acgattccgt acaccaaaac agtcatgccg atgccgattg ccgccattac gagcatgcgc
     3361 gtcatgtcgg tgcagaaact tgtgcagcag cttttctacg ccttcaaagc acagataaat
     3421 gccgcctgcc gtcaaaagcg gcgtaatgag ttgcggcagg aaggcggaaa gcagcagggg
     3481 cgcaggcacc aaaaccggct tgttggaaaa agaacctttc gccatcgacc aaacaatcgg
     3541 caactcgcgt tctgccgata cgcccgtaac ccggttggca ttgggtgtca aatcgtcgcc
     3601 gaccacgccg gcggttttct ttgcggcggc tttggtcatc agggcaacat cgtccaaaac
     3661 ggcggtgatg tcgtccggca gggtaaatag tgaggcaaat gccattaaag aatcctgaaa
     3721 tgcggcgcaa agtccgacat tatataggag aacgcggatt tgggcggttt caggcggcat
     3781 gaaacaggaa aatgccgtct gaaggcggtg gcggacgtga agtaaagttt cgtgaaaaaa
     3841 atgccgtgtt acagtctttc gattttaatt taatgaattt taagggagag tcgttagcgt
     3901 ggattggatg ggcagtctgt tcctgccagg tggcgcactg ttgttcctga gcgtggtttc
     3961 gaccactttg tccgcacgtt tgggaatgcc tttgctgctg gtttctcccg caggcgtgtt
     4021 ggacggggcg gcggaagcct tggcgattgc ggcgtttctg atgctggtcg cgcgtccgct
     4081 ggcagtgttc ggcggtttgt ggaaattcaa ttatagcctg cgtgaaaagg cgtatagccg
     4141 aatagaaatg cagtccgaca ccgtgcttca ggcggggatt tggcgtggta catcctgccc
     4201 gacggcaagg tcgatatagt g
//
LOCUS       DAXHSJ010000158         4154 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000158 DAXHSJ010000000
VERSION     DAXHSJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.158"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(20..682)
                     /locus_tag="ACFM8U_001803"
     CDS             complement(20..682)
                     /locus_tag="ACFM8U_001803"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6334993.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLLKLGTGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSVKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QS"
     gene            complement(720..1547)
                     /locus_tag="ACFM8U_001804"
     CDS             complement(720..1547)
                     /locus_tag="ACFM8U_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC6334994.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            1825..2448
                     /gene="lolA"
                     /locus_tag="ACFM8U_001805"
     CDS             1825..2448
                     /gene="lolA"
                     /locus_tag="ACFM8U_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC6334995.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            2721..3860
                     /locus_tag="ACFM8U_001806"
     CDS             2721..3860
                     /locus_tag="ACFM8U_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6334996.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTGQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLLPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            3993..>4154
                     /locus_tag="ACFM8U_001807"
     CDS             3993..>4154
                     /locus_tag="ACFM8U_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6334997.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT          996 a         1169 c         1125 g          864 t
ORIGIN      
        1 tttgtggggc ggatattttt caggactgta ttttgtccag acattcgagc agatcgagcg
       61 gcgtgcggat gtggaaatcc gcctgccatg agccggtatc gtcttcggga gcgatgtagc
      121 cccattcggc gaggacggtt ttcatgccgg cgttgcggcc tgcttggatg tcgcgttcgg
      181 catcaccgac gtagagtgtg tgttgcgggt cggcgtggat ttgtccgcac gcatacagca
      241 tgggtttgac gctgggcttg ggttcgccgc aggtgtcgcc gctgacgacg acggcgggtg
      301 ggatgatgaa gccgagtttg gggacgagtt tgtcggtgaa gcgcatgggt ttgttggtga
      361 tgatgcccca tttgatgccg cgttggttga gttcggcaat gagttcgttc acgccgtcaa
      421 agagctcggt gtcttgggcg tagcggcggt cgtattcctc cagatattcg gtgcgccatc
      481 gggcatagtc ggggtggtcg ggggtgatgc ctgtgccgag cttgagcagt cctgccgcgc
      541 cgtggctggc ttgggtgcgg atctcgtcca tgctttttgc aggtagtccg tggcgagcga
      601 gcagggtgtt gagtgcgcct ccgaggtcga gggcggtgtc ggcaagtgtg ccgtcgaggt
      661 cgaagagtac ggcgtgtatc atgtgtgttc cttttttata aagtgcggga cgaagggttt
      721 cagacggcat gtttattttg tttcaaaccc tgttcgaaat cttccagcat atccaattca
      781 aagcggctga agcccgcttt ttcgcgcgct tcgatgttca catagccgcg gaagataaac
      841 atatcgtaac gggcaatcag actgcggaac agggtaatcg gttccaaacc gcgttcgcgg
      901 cagaggtgtt gataccaatg gttgccgatg gcgacatgac ccacttcgtc gcggtaaatg
      961 atgtccaata cgccgcaggt ttccgaatcg ccgcgctgcg ccaccttcgc gcgtatcccc
     1021 ggcgtaacgt ccagcccgcg cgcttccaac acgcgcggta ccaacgccat acgcaacagc
     1081 gggtcgtagg cggttttgta cgccatatcc cacaaatggt tgtgcgcctc gaaactgccg
     1141 taatcgaagc cgaaagcgcg caatctgtcg cgcatcaggc ggaaatggta cacctcttcc
     1201 ttcgccactt tcacccagtc gcggacaaac tgaaacggca gcgtgcggaa acggtatgcc
     1261 gcgtccaaag ccagattgac ggcgttgaat tcgatatgcg caatcgcgtg cagcatcgcc
     1321 gcatagcctt ctgccgtgtt cattttgcgt ggcgtcagtt gcgacggcgc gaccaaaaca
     1381 ggcttgtccg gccgtcccgc gcgggggaaa tctgccggtg gcgcgtttgc ttccgccccg
     1441 tccgcatttt gaacggcggc aaacgcctca tccgtcagcc gtcctttttc atctgggtcg
     1501 cccgaaagca gggcgcgttc cagcaaagca taaatatcga gtttcatctc aagtccgccg
     1561 tgttcggaaa acaaatatta tagcgtttta aaaaaacaag atgaggcata taatctccgc
     1621 gattcggcat tccgcgccca aaccgtcaaa tcacgcggca cacaattaaa gggcagcgtg
     1681 gcgcgcctac cttttccgag cggtcaaaaa aatcagccct cggaaaacgc tgtttgtaaa
     1741 atgcaaaccg cccgtaacgc cgcccgtatg attgttttgc cgcgccgata ctttacgcca
     1801 cacccatccc aacaaggaaa aataatgatg aaaccgcaca acctgttcca atttctcgcc
     1861 gtttgctccc tgaccgtcgc cgtcgcttcc gcacaggcgg gcgcggtaga cgcgctcaag
     1921 caattcaaca acgatgccga cggtatcagc ggcagcttca cccaaaccgt ccaaagcaaa
     1981 aagaaaaccc aaaccgcgca cggcacgttc aaaatcctgc gcccgggcct cttcaaatgg
     2041 gaatacactt cgccctacaa acagactatt gtcggcgacg gtcaaaccgt ttggctctac
     2101 gatgtcgatt tggcacaagt gaccaagtcg tcccaagacc aggccatagg cggcagcccc
     2161 gccgccatcc tgtcgaacaa aaccgccctc gaaagcagct acacgctgaa agaggacggt
     2221 tcgtccaacg gcatcgatta tgtgctggca acgcccaaac gcaacaacgc cggctaccaa
     2281 tacatccgca tcggcttcaa aggcggcaac ctcgccgcca tgcagcttaa agacagcttc
     2341 ggcaaccaaa cctccatcag tttcggcggt ttgaatacca atccccaact ctcgcgcggc
     2401 gcgttcaagt ttaccccgcc caaaggcgtg gacgtgttga gcaactgatg ccgtccgccc
     2461 cgatgccgtc tgaaagccgc cgaggcttca gacggcattt tcacgcaggc ggaacaatgt
     2521 cccgcattac cgcccgatcg ggcatcggaa cggcaaaccc gtgaaaatta acggttgcgc
     2581 cccggttgtt tttgccgttt aatgcaaacc ttgctgcatc aagggccaag aaagccgacc
     2641 ggccgccccc acagcttccg atgcaggcgg ccaggccgtc cctgcaatgt tttttatttt
     2701 tgaacgaaag gtcgaaaacc atgaaaaaaa cactggtggc ggcggcaatc ctgagcctcg
     2761 ccttgactgc gtgcggcggc ggaagcgata ccgccgccca agccccctcc gccaagcctg
     2821 aagccgaaca atcgggcaaa ctcaatatct acaactggtc ggattatgtc gatcccgaaa
     2881 ccgtcgccgc ctttgaaaaa gaaaccggca tcaagacgcg ttccgattat tacgacagca
     2941 acgaaacact ggaggcaaaa gtcctgaccg gcaaatccgg ctacgacctg accgcgccgt
     3001 ccatcgccaa cgtcggccgg caaatcaaag cgggcgcgta tcagaaaatc gacaaggcgc
     3061 aaatccccca ttacggcaac atcgataaag atttgctgaa aatgatggaa gccgtcgatc
     3121 cgggcaacga atacgccgtc ccctatttct ggggcatcaa caccttggca atcaacacgg
     3181 ggcaggtgca aaaggcattg ggtacggaca agctgcccga aaacgaatgg gatttggtgt
     3241 tcaaacccga atacaccgcc aaactcaaat cctgcggcat cagctatttc gacagcgcaa
     3301 tcgaacagat tcccttggcg ttgcactatt tgggcaaaga ccccaacagt gagaatcccg
     3361 aagacatcaa agccgccgtc gatatgatga aagccgtccg gggcgacgtg aaacgcttca
     3421 gctcttccgg ctatatcgac gatatggcgg caggcaacct gtgtgccgcc atcggttacg
     3481 gcggcgattt gaacattgcc aaaacccgtg ccgaagaagc cgcaaacggc gtggaaatca
     3541 aagtattgac cccgaaaacc ggcgtgggcg tgtgggtgga ttcctttatg attccgcgcg
     3601 acgcgcaaaa cgttgccaac gcccaccgct atatcgacta cacgctcctg cccgaggtgg
     3661 cggcgaaaaa cggcagcttc gttacctacg cacccgccag ccgccccgcg cgcgagctga
     3721 tggatgaaaa atacacctcc gatgcatcga ttttcccgaa caaagaactg atggaaaaaa
     3781 gtttcatcgt atcgcccaaa tccgcagaat ccgccaaact gggcgtgaag ctgtggcaag
     3841 ggctcaaagc gggcaaataa ccgcaacccc tgccgtctga aacctttcgg gcggcaggaa
     3901 acggcgcgtc cgttatcaaa caggggggcg tttcccctcc tgccggttat gattgggtta
     3961 agattaaaat gatttagtaa aatgagaaag atatggattt aagtatcgta gttcccattt
     4021 ataatgtcga aagttatttg gaagcgtgtt taagttccat agaacctata ttaagtaatg
     4081 aaaatgtcga acttatcctt gtgaatgacg ggtcaaaaga cggaagtgaa gatatatgtt
     4141 ataaatatat agat
//
LOCUS       DAXHSJ010000159         4128 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000159 DAXHSJ010000000
VERSION     DAXHSJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.159"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            43..675
                     /locus_tag="ACFM8U_001808"
     CDS             43..675
                     /locus_tag="ACFM8U_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC6334998.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="ACFM8U_001809"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="ACFM8U_001809"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC6334999.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIGKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIESLEIPVEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2198..4066
                     /locus_tag="ACFM8U_001810"
     CDS             2198..4066
                     /locus_tag="ACFM8U_001810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6335000.1"
                     /translation="MQTVRYRPEIDGLRAVAVLSVIIFHLNDRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTASSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHESLLKHSHGNALQ"
BASE COUNT          953 a         1118 c         1053 g         1004 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcggtc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 atggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttccgaa tacggcctga
      841 tgaaagcgcg cgtcaaagtc gaattaaact ggctcaaagc cctcgccgcc gagccgaaga
      901 ttgccgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccaccacc aatcacgatg
     1021 tcaaagccat cgaatattgg ctgaaaaaac gttttgccga agtgcccgaa gtcgccgccg
     1081 tgagtgagtt catccacttc gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgcccaa actggcagaa atcatcggca
     1201 aactcaccgc tatggcgcac gaccttgccg ccgtcccgat gatgagccgc acccacggcc
     1261 agcccgccac gccgaccact ttgggcaaag aaaccgccaa tgtcgtgtac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gcgcaagagt tcctcggcaa aatcaacggc gcggtcggca
     1381 actacaacgc ccatatggtc gcctatcctg atgtagattg ggaaacccac tgccgcaact
     1441 tcgtcgaaat cagcctcggt ctgaccttca acccctacac catccaaatc gaaccgcacg
     1501 actatatggc ggaattcttc caaaccctca gccgcatcaa cacgattctc atcgacttta
     1561 accgcgacgt ttggggttat atttcattgg gttacttcaa acaaaaagtc aaagcaggcg
     1621 aagtcggttc ttccaccatg ccgcacaaag tcaaccccat cgactttgaa aactccgagg
     1681 gcaacctcgg tatggcaaac gccgtattgg gctttttgtc cgaaaaactg ccgatttccc
     1741 gctggcagcg cgacctgacc gacagcaccg tattgcgcaa tatgggcgta ggcgtgggct
     1801 atgccgtatt gggtttcgcc gcccacctgc gcggtctgaa caagctcgaa cccaaccccg
     1861 ccgcgcttgc cgccgatttg gatgccactt gggagctgct cgccgagccg attcaaaccg
     1921 taatgcgccg ttacggtgtc gccaatcctt acgaaaaact gaaagacctg acgcgcggca
     1981 aaggcggcat cacgcccgaa gtgctgaaag gctttatcga atcgttggaa attccggtag
     2041 aagccaaagc aaaattgctt gagctgactc ctgcactgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacgggggc gatattgatg caaactgtcc gatacaggcc
     2221 tgaaattgac ggattacggg ctgtcgccgt gctatccgtc attattttcc acctgaatga
     2281 ccgctggctg cccggaggat tcctgggggt ggacattttc tttgtcatct caggattcct
     2341 cattaccggc atcattcttt ctgaaataca gaacggttct ttttctttcc gggattttta
     2401 tacccgcagg attaagcgga tttatcctgc ttttattgcg gccgtgtcgc tggcttcggt
     2461 gattgcctct caaatcttcc tttacgaaga tttcaaccaa atgcggaaaa ccgtggagct
     2521 ttctgcggtt ttcttgtcca atatttatct ggggtttcag caggggtatt tcgatttgag
     2581 tgccgacgag aaccccgtac tgcatatctg gtctttggca gtagaggaac agtattacct
     2641 cctgtatcct cttttgctga tattttgctg caaaaaaaca aaatcgctac gggtgctgcg
     2701 taacatcagc atcatcctat ttctgatttt gactgcctca tcgtttttgc caagcgggtt
     2761 ttataccgat attctcaacc aacccaatac ttattacctt tcgacactga ggtttcccga
     2821 gctgttggca ggttcgctgc tggcggttta cgggcaaacg caaaacggca gacggcaaac
     2881 agcaaatgga aaacggcagt tgctttcatc actctgcttc ggcgcattgc ttgcctgcct
     2941 gttcgtgatt gacaaacaca atccgtttat cccgggaatg accctgctcc ttccctgcct
     3001 gctgacggca ctgcttatcc ggagtatgca atacgggaca cttccgaccc gcatcctgtc
     3061 ggcaagcccc atcgtatttg tcggcaaaat ctcttattcc ctatacctgt accattggat
     3121 ttttattgcc ttcgcccatt acattacagg cgacaaacag ctcggactgc ctgccgtatc
     3181 ggcggttgcc gcgttgacgg ccggattttc cctgttgagt tattatttga ttgaacagcc
     3241 gcttagaaaa cggaagatga ccttcaaaaa ggcatttttc tgcctctatc tcgccccgtc
     3301 cctgatactt gtcggttaca acctgtacgc aagggggata ttgaaacagg aacacctccg
     3361 cccgttgccc ggcgcgcccc ttgctgcaga aaatcatttt ccggaaaccg tcctgaccct
     3421 cggcgactcg cacgccggac acctgcgggg ttttctggat tatgtcggca gccgggaagg
     3481 gtggaaagcc aaaatcctgt ccctcgattc ggagtgtttg gtttgggtag atgagaagct
     3541 ggcagacaac ccgttatgtc gaaaataccg ggatgaagtt gaaaaagccg aagccgtttt
     3601 cattgcccaa ttctatgatt tgaggatggg cggccagccc gtgccgagat ttgaagcgca
     3661 atccttccta atacccgggt tcccagcccg attcagggaa accgtcaaaa ggatagccgc
     3721 cgtcaaaccc gtctatgttt ttgcaaacaa cacatcaatc agccgttcgc ccctgaggga
     3781 ggaaaaattg aaaagatttg ccgcaaacca atatctccgc cccattcagg ctatgggcga
     3841 catcggcaag agcaatcagg cggtctttga tttgattaaa gatattccca atgtgcattg
     3901 ggtggacgca caaaaatacc tgcccaaaaa cacggtcgaa atatacggcc gctatcttta
     3961 cggcgaccaa gaccacctga cctatttcgg ttcttattat atggggcggg aatttcacaa
     4021 acatgaaagc ttgctcaagc attcacacgg caacgcattg cagtagcctg ccttcttgtc
     4081 ggatattgcc tttggcagcc tatgccgctg tttgcccttc ggggcggc
//
LOCUS       DAXHSJ010000160         4127 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000160 DAXHSJ010000000
VERSION     DAXHSJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.160"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(179..1429)
                     /gene="glyA"
                     /locus_tag="ACFM8U_001811"
     CDS             complement(179..1429)
                     /gene="glyA"
                     /locus_tag="ACFM8U_001811"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC6335001.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     DAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1495..1707)
                     /locus_tag="ACFM8U_001812"
     CDS             complement(1495..1707)
                     /locus_tag="ACFM8U_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335002.1"
                     /translation="MADKDVVGAGYLIDFAQTAENLPFNVLLLISLVLNKGDETLKTS
                     MLNKLPMLNKLPDNAKENLRIMGYLP"
     gene            complement(1928..3703)
                     /gene="ggt"
                     /locus_tag="ACFM8U_001813"
     CDS             complement(1928..3703)
                     /gene="ggt"
                     /locus_tag="ACFM8U_001813"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFC6335003.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKVLHAGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGNYELETGTTAVQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3911..>4127)
                     /locus_tag="ACFM8U_001814"
     CDS             complement(3911..>4127)
                     /locus_tag="ACFM8U_001814"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HFC6335004.1"
                     /translation="RHAPVSPMWIFSACSRGADPIGRPFAFVNILENPEIRSTLPLIA
                     NRPTFPQLWVNGELIGGSDIITNVPIR"
BASE COUNT         1037 a          968 c          971 g         1151 t
ORIGIN      
        1 gatatgtttg gcaatcatgg cttgcgcggt ttgccggaag aaggtgctca tggaaaatcc
       61 cctaaatgtc ttggtgggaa tttaggggat tttggggaat tttgcaaagg tttccgcctg
      121 aaacattatg agatttcagg cggcattgga ttgcttagcg gaatattttt aaaaaggctt
      181 acgcgccgta aacggggtat ttattgcaca aagcagttac ttgttcgcgg actttggcga
      241 ggttggcttc gtcttcgggg ttagacaata cgtcggcaac caagttcgcc aatacgcgcg
      301 cgtcggcttc gttaaaaccg cgtgtggtca tggcggcgga accgatgcgg atgccggagg
      361 taacgaaggg tttttccgga tcattcggga tggcgttttt gttgacagtg atgtgcgctt
      421 tgcccaaagc ggcttcggcg gctttgccgg taattttcat cggttgcagg tcgacgagga
      481 aaacgtggct ttcggtgcgg ccggaaacga tgcgcaaacc gcgtttgacc agttcttccg
      541 ccatggcggc agcattgatt ttcacttgtt ttgcgtattg tttgaactcg ggttgcaacg
      601 cttctttaaa cgctacggct ttggcggcga tgacgtgcat cagcggaccg ccttgcaggc
      661 tcgggaagat ggaagagttc agtgcttttt cgtgtgtgtt gtcgcggcac agaatcacgc
      721 cgccgcgagg gccgcgtagg gttttgtggg tggtggtggt cacgaagtcg cagaatggca
      781 cggggttggg atattcgcca ccggcaatca gtccggcata gtgcgccata tcgacaaaga
      841 ggtatgcgcc gactttatcg gcgatttcgc ggaattttgc ccagtcgatt tgcaacgcgt
      901 aggcagacgc acccgccaca atcattttgg gtttgtgttc gagcgcgagg cgttcgactt
      961 cggcataatc gagcacttcg ttttcatcca aaccataagt aacagcgttg tagagtttgc
     1021 ctgagatatt aacgctcgcg ccgtgggtca ggtggccgcc gtgcgccaga gacataccca
     1081 aaatggtgtc gcctggtttt aaaacggaag cgtacacggc ttggttggct tgcgagccgg
     1141 agtgcggttg gacgttggcg tattgcgcgc cgaacagttt tttgacgcgg tcaatcgcca
     1201 attgttcgac aatatcgacg tattcgcagc cgccgtagta gcgtttgcca ggatagcctt
     1261 cggcgtattt gttggtcaat tgcgaacctt gcgcgtccat tacggcgcag ctaacgtagt
     1321 tttcggaagc aatcagctcg acgtggtctt gctggcgttg gtcttcttgg gcaatggctg
     1381 ctgccaaatc ggggtcgtat tgtgcgaggg taacgctttt tgaaaacatg ttctcggctc
     1441 ctttgtgtaa tcagggtatc atgagtgttt tttgtataaa aaatacttca atacctaagg
     1501 tagatagccc ataatgcgta aattttcttt ggcattatca ggtaatttat ttaacatggg
     1561 taatttattt aacatgctgg tttttagcgt ctcatcacct ttatttagta caagactaat
     1621 caaaagcaat acattaaatg gtaaattttc ggcagtttgt gcaaaatcaa tcaaataacc
     1681 agcaccaacc acatctttat cagccataac ccgctgatac accacttgct aagacaactc
     1741 tcctgcccca ccatgtctta aaatcagatg aatggcttgt aatgctgtgt ttggtgtctc
     1801 acaaattgcc aaaatctgat cagaagcttg cttgatggct tgtatttttg tttgattact
     1861 cacaatttcc ccctatttta ataattaact taaatgcggt caaattcaca aaatacaagc
     1921 ttcacctcta atcacccatc actcgacctt ctcggcgtgg atcggcacca ccaaccagcc
     1981 tgcttggctc gataataatg gcttgaacac ctgaatttag ctcacgcaca tcagtcttat
     2041 agcccaaatc atttaatgct tgttgccact ggacggcggt tgtacccgtt tctagttcat
     2101 agttaccaaa gcgatttaat aaattgggtg cactgatggc atcttggata tccatattcc
     2161 agtcactatg tgccacaatc gtcttagcga catagccaat gatacggcta ccacctgggg
     2221 agccgattgc catataaggc ttgcctgctt taaatacgat ggttggtgcc attgaggagc
     2281 gtggtctctt gccgggctcg acacgattgg cgacctgttt gccctgcttt attggctcaa
     2341 aactaaagtc tgtcagctca ttattcagca ggtagccatt tgccatcaaa gttgagccaa
     2401 acgcattttc aatggaagtc gtcattgata gcacattgcc cgccttatcc acaattgata
     2461 tatgactggt agaaggtaac tcaatcgctt gtgaggacac ccactcatga ataaaatcgc
     2521 ctgcagatac gctaggcaat gccttatccg actgctcaag cagctggctg cgatgtttta
     2581 ggtagtcttt agaaatcaac tggcgaatgg gtactggtac aaaatcaggg tcgcccaaat
     2641 atacatcacg atccgcaaac gcaagcctag aagcgtcgcc caagagacgt aaaccttcag
     2701 catcataccc cacctgattg ggtgaaaatt catttaaaat ccccaaaatc tgacccacag
     2761 caatcccacc tgagcttggt gcacccatac cgcatacttc ataaatacga taagtcacac
     2821 aaacaggcgg gcgttccacc acttgataat cagataaatc ttgtaaggat aattgaccgg
     2881 ggttatcctt agcattttgg acaactgaaa cgatattttg ggcatattta ccagcatgca
     2941 gaacttttgc accttgagct gctaacgcct gaacactgtc agcaaattct aaatttttca
     3001 gcaagctgcc tgcttgtagc ggcacaccat tcggcaaaaa ataagcggct gtttttggat
     3061 agcgtgccaa atgctgctga ttttgctcaa ccgagatggc aagccttggc gacacctcaa
     3121 agccttgttt tgccaagcgg atcggcgtat caaataattt tccccaaggc aatacaccgt
     3181 atcgctgatg tattgtctcc atcagtttag ggatagcagg cgtacccacc gagcgaccac
     3241 cgaccaccgc ttccataaat ttcaatggtt gaccatcttt atccaaaaat aattccggcg
     3301 tcgcacgcat cggtgccgtc tcacgcccat caaatgtggt caatgttttg gcggtattat
     3361 cccaatacaa cacaaatgca ccaccgccca agcctgacga ctgtggctct accaagctta
     3421 gtgtcgtctg caccgccacc atcgcatctg cagcgctacc gccttgcttt aagatatcat
     3481 agccagcttg tgttgctaat ggattggctg acgctaccat aaaatcactt gcaatcacct
     3541 gcttttgttc ggtcagtccc gttgcatgtt caggcgtgtg agcgtctgca cctgtaatga
     3601 cagcagaatg agtattaacc ttaccttgat tggcatggat gacttgacat ccagagattg
     3661 tcatagacat tatcaatgca gtcaataaat atgttttagc cacaagcact ccttcgcctg
     3721 agtttgattg atgattcata caaggcatgc tgattattgt atttaatatg actaaataat
     3781 tcaatccaaa ctatcaatct tgaccatcaa aaaaagaccg ctaatgtcat cagcagtctt
     3841 ttttgatatt tattttaaga tattaagtaa tcagaccttt gggctatgct cttcaatgag
     3901 tggttttagc tcacctgatt ggtacatttg tgataatatc actaccaccg attaactcac
     3961 cattaaccca aagctgtgga aaggttggcc gattggcgat gagtggtaga gtactgcgaa
     4021 tttctgggtt ttctaggata ttaacaaaag caaagggtct gccaattggg tcagcacctc
     4081 tactgcacgc gctgaaaatc cacattgggg aaactggggc gtgcctt
//
LOCUS       DAXHSJ010000161         4074 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000161 DAXHSJ010000000
VERSION     DAXHSJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.161"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(379..1191)
                     /gene="murI"
                     /locus_tag="ACFM8U_001815"
     CDS             complement(379..1191)
                     /gene="murI"
                     /locus_tag="ACFM8U_001815"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC6335005.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            1381..1842
                     /gene="tsaE"
                     /locus_tag="ACFM8U_001816"
     CDS             1381..1842
                     /gene="tsaE"
                     /locus_tag="ACFM8U_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC6335006.1"
                     /translation="MSDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            1839..3089
                     /locus_tag="ACFM8U_001817"
     CDS             1839..3089
                     /locus_tag="ACFM8U_001817"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6335007.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYLNTSVLKRG"
     gene            3169..3987
                     /locus_tag="ACFM8U_001818"
     CDS             3169..3987
                     /locus_tag="ACFM8U_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6335008.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDRPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIGNPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDIFRNFSL"
BASE COUNT          973 a         1188 c         1001 g          912 t
ORIGIN      
        1 gaaacctcaa aaaaaccgaa accgaacgga ctggattccc gcctgcgcgg gaatgacggg
       61 tttcgagatt acggtgttgt tgggaatgat ggaaaatggt ggggattgtg taaaaaatgc
      121 catctgaagc ctttcagacg gcatttgcgg cattcggaca tttaaaaatc ccatcattcc
      181 cgcgcaggag ggaatccgaa tacatctgca cagaaacctg catcccgtca ttcccgcgaa
      241 agcgggaatc tagaacgtaa aatctaaaga aaccgtttta tccgataagt ttccgcaccg
      301 acaggtctag attcccactt tcgtgggaat gacgggattt taggtttctg taatctcggg
      361 cagctttccg tcatcgtttt aacccaaaga caccatttca atctgctcca tcgtcctgcc
      421 cagaaaacgc tcgccgatgg ttctgaattt caaaggaata tcgctgacgt aaaaacggta
      481 gtcgggattg ttgttgtcgg tattgagcaa tccttcctgc gcaaggacgc gtgcggtttc
      541 ttcggcagtc gtaatcgcgg aatcgaccag tgcgacattg tgcgcctccc tgccgattaa
      601 gggtttgagc aagggaaagt gcgtgcagcc caacaccagc gtatcgatgc cgtccgcaag
      661 caatggtttg aggtattcgc atacggtcag gcgggtaact tcatgttcca accaaccttc
      721 ctccaccaaa gggacgagca gcggcgcggc ctgcgtgcgg acgagcgtat cggggttgtt
      781 cctgtggatg gcgcgcgcat aggcgttgct gttgactgtc gtattggtgg cgataatgcc
      841 gattttattg ttgcgcgtcg ttgccagcgc ggctttcgcg ccggcggaaa tcacgtccaa
      901 aacgggcata ttgccggttt tttgacggat tttctgcccc gccaccgccg caatcgtatt
      961 gcacgcgata accatcgcct tgacatcgtg ttccaataaa aaatcgacaa tctgcatcga
     1021 gaaattttcg atggtcgcct tagatttcgt cccgtaaggc acgcgcgccg tgtcgccgaa
     1081 ataaatgatg ttctccatcg gtagccgttc catcagcgct cgcacattgg tcaaaccgcc
     1141 gattcccgag tcaaaaacgc cgatgggtcg ctgcctgccg atattttcca ttctttttcc
     1201 aatccgtcct aaacatacaa tccgcgtatt gtacacgcgc cgctttttct tgacagccgt
     1261 tgccgtctga aagcaggatg cggatttggc tgttataatg tacggcaatg ctgccgccgg
     1321 gcgcacggtt tgccgaccgc ctgcccctcc gatccgccac ataaaagttg taaagcccct
     1381 atgtctgatt ttcctcccgt ttcccgattc cttgccgacg aagccgccac actcgatttg
     1441 ggtgcggcgt ggtcttcccg tttaaacgca ccgctggtca tttatctcga aggggatttg
     1501 ggtgcgggca aaaccacgct gacacgcggc atcctgcgcg gattgggtca tcagggcgca
     1561 gtcaaaagcc cgacctacgc catcgtcgaa tcttatccgc tggaacgctt caccctgcac
     1621 catttcgacc tctaccgctt ctcgttcccc gaagaatggg aagacgcggg gcttgacgaa
     1681 ctgtttgccg caaacagcgt ctgcctgatc gaatggccgc aacagggagg cgaatttacc
     1741 ccgcccgccg acatcaccgc aacattgaca cacgacggcg gcggcagaaa atgcctgctg
     1801 accgcccata ccgaacgagg acgcgaaagc ctgccgctat gaccaaactg acacgaagac
     1861 aaatcatccg ccgcaccgcc ggcacactgt tcgccctaag ccccatcgca tccgccgttg
     1921 ccaaaacggt acgcgcacca caattcactg ccgcacggat atggccatcg cacacctaca
     1981 cccgcctgac gctggaaagc accgccgcgc tcaaatacca gcacttcgcg ctcgacaacc
     2041 cgggcaggtt ggtcgtcgac atacaaaacg cgaacatcaa taccgtattg cacggactgt
     2101 ctcagaaggt tatggcggac gaccctttta tccgcagcat acgcgcgggt cagaacacgc
     2161 cgaccaccgt ccgcctcgtc atcgatttga aacagccttc ctacgcgcaa gttttttccc
     2221 tcccccccgt cggcggcttt aaaaaccgcc tcgtcgtcga tctctatccg caagggatgg
     2281 atcccgacga tccgatgatg gcgctgctta acggcagcct gaacaaaacc cgtcgcggct
     2341 cccctgaagc cgacctcgct caaaacactc ccgcacaaac cgaacgcggc aaaaacgggc
     2401 gcagacccgt cattatgatc gaccccggac acggcggcga agaccccggc gccgtcagcc
     2461 cgggcggttt gcaggaaaaa cacgtcgtcc tctccatcgc ccgcgaaacc aaaaaacaat
     2521 tggaagcctt gggttacaac gtctttatga cgcgcaacga agacgtgttc atcccattgg
     2581 gcgtgcgtgt cgccaaaggg cgcgcacggc gggcggacgt attcgtctcc atccacgccg
     2641 acgctttcac cagcccctcc gcgcgcggaa cgggggttta tatgctgaac accaaaggcg
     2701 cgaccagctc tgccgccaaa ttcttggaac aaacccaaaa taatgccgac gcggtcggcg
     2761 gcgtaccgac cagcggcaac cgcaatgtcg ataccgccct gctcgacatg acccaaaccg
     2821 ccacgctgcg cgacagccgc aaactcggca aactggtgct tgaagaattg ggcaggctca
     2881 accacttgca caaaggcagg gtggacgaag ccaatttcgc cgttttgcgc gcgcccgata
     2941 tgccgtctat cctggtcgaa accgccttcc tgtccaatcc tgccgaagag aagctgctgg
     3001 gcagcgaatc cttccgtcgg cagtgcgccc aatccattgc ctcgggtgtc caacgctacc
     3061 tcaatacatc cgtattgaag cggggttgat tgaaaaggcg gtgtttcaat atagttgtcg
     3121 gatgaaggca ggcataaacc tacttccgcc ccaaaccgcg tttttatgat gaaaggtatg
     3181 gataaattga gataccaaca ggatttttta aatatccgcc ccatttttac agccggcgaa
     3241 caagaatacc tgaccgaatt gtccgatcgg cttcctttat ctgttttaac ggactccgtg
     3301 cgtaatattg aagaaatcgg tatcgatttt gtgtacagtt ctgccaaatt ggaaggaaac
     3361 acttataatc aatacgatac gcaggcattg cttaaactcg ggcaaaccgc aggcgggaag
     3421 ttatattcgg atgccgtaat gttaatcaac ctgagggaaa gttaccggca cctgctgtcc
     3481 ggtttggacc gccccgatcc gtttgactgg ttggatttct taaaaaccac ccacagcctg
     3541 atttcggaaa acttactgga aaaaggctcc ggcggcgtag tccgccgcga cagcgttact
     3601 ataagcggga cagactacac gcctttatcc aacccgcaaa gtttggatac ggaattaaaa
     3661 tggttgcttc aagaagcccc aaaaatagga aatcccttcg accgcgccgt ttatctccat
     3721 aataatttgg cgtatcttca atattttaaa gattgcaata aacgtactgc cagaaactgc
     3781 atgaccttgt cgctgatgcg ctccggtttt tttccttgcg tattctcccc cgacagttat
     3841 cccgcctatg ccgaggctgt cgtagcctat tacgaaaccg gcgattacgg tttattcaag
     3901 aaatatttta tttcggcata cgaaaatacc gtaaataagt acggcccaca gcctgatatg
     3961 gatattttcc gcaacttctc cctttaattc caaggggttg ttatccgtat cttccgcacg
     4021 cggcaaatgc ggcggcaaaa tggacacgcc cctcccttac cttcggttat agtg
//
LOCUS       DAXHSJ010000162         3994 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000162 DAXHSJ010000000
VERSION     DAXHSJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.162"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..904
                     /locus_tag="ACFM8U_001819"
     CDS             <1..904
                     /locus_tag="ACFM8U_001819"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6335009.1"
                     /translation="AKVAATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAV
                     AEAKANLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNPAL
                     KNLSPAQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQ
                     YRAQHILIKADSENAAVSAESTIRKIYDQARNGTDFAGLARRYSQDASAGNGGDLGWF
                     ADGVMVPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVREAGTPQERVRNAVRQYIF
                     QQKAEQSTVNLLRDLHSGAYIDIR"
     gene            complement(2006..3886)
                     /locus_tag="ACFM8U_001820"
     CDS             complement(2006..3886)
                     /locus_tag="ACFM8U_001820"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HFC6335010.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFTE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQLLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTSFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVKPGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAYRPAVSIYFDVDSE
                     FNTGEPTCKIEAVSIAENLRIQTIEPHFNAETGLDEAGEMMFAHHQDLIWFYQFAIAL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     SAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGKTAGN
                     AV"
BASE COUNT          904 a         1017 c         1138 g          935 t
ORIGIN      
        1 tgccaaagtt gccgccacgg cgcaaaaaga agccgcaccc gcacaacagc agggcggtat
       61 ccgcttttca gacggcattg ccgccgttgc cgacaacgaa gtcatcacgc gccgccggct
      121 tgccgaagcc gttgccgaag ccaaagccaa cctgcccaaa gacgcgcaga taagcgaatc
      181 cgagctgtcc cgacaggtgc tgatgcagct tgtcaaccaa tccctgattg tacaggcggg
      241 caaacgccgc aacattcaag caagcgaagc ggaaatcgat gccgtcgtcg caaaaaatcc
      301 cgccctcaaa aacctcagcc ccgcccaacg ccgcgatttt gccgacaaca tcattgccga
      361 aaaagtccgc cagcaggcag tgatgcagaa cagccgcgtg agcgaagctg aaattgatgc
      421 cttcctcgag caggcgcaaa aacaaggcat caccctgccc gaaggcgcac cgttgcgcca
      481 ataccgcgcc caacacatcc tgattaaggc cgacagcgaa aacgccgccg tcagcgcaga
      541 aagtaccatc cgcaaaatct atgaccaggc gcgcaacggc acggatttcg caggtttggc
      601 acgccgatat tcccaagacg caagcgcggg taacggaggc gatttgggct ggtttgccga
      661 cggcgtgatg gttcccgcct ttgaagaagc cgtccacgcg ctcaaacccg gacaggtcgg
      721 cgctcccgtc cgcacccaat tcggctggca catcatcaag ctgaacgaag tacgcgaggc
      781 gggcacgcct caggaacgcg tccgcaacgc cgtgcgtcag tacatcttcc aacaaaaagc
      841 cgaacagtca accgtcaacc tgttgcgcga cctgcattcc ggcgcgtaca tcgacatccg
      901 ctgaaacggt ttgaatcaaa aagccatacc gatcggcaaa aatccgggcg gtatggcttt
      961 tttgaatttc gagttacttt tacaccgtca ttcgtcattc ccgcgaaagc gggaatctag
     1021 aaacgaaaag taacaggaat ttatcgggaa tggctggaat ttaaaggtct ggattcccgc
     1081 tttcgcggga atgacgggat tttgggttgt ggtaatttat cggaaaaaca aaaaaaccta
     1141 tgccgtcatt cccgagcagg cgggaatccg gttatttaaa actgcagaaa tttatccgaa
     1201 gcaacaacaa tctttccatc gtcattcccg cgtaggcggg aatctagcaa cgaaaagtaa
     1261 caggaattta tcgggaaaac agaaacctct atgccgtcat tcccgcgaaa gcgggaatct
     1321 aggacgtaga atctaaagaa accgttttat ccgataagtt tctgtgccga agggtctgga
     1381 tttccgcttt cgcgggaatg acggcgcata agttcccgtg cggacagacc tagattccca
     1441 cctgtgtggg aatgacgatt cagaagttgc ccgaaactca aaaaaaaacg aaaccgaacg
     1501 aaccggattc ccgcttttac gggaatgacg ggattttggg ctgtggtaat ttatcgggaa
     1561 aacggaaacc cctatgccgt cattcccgcg caggcgggaa tctaggacgt agaatctaaa
     1621 gaaaccgttt tatccgataa gtttctgtac cgaagaatct ggattcccgc tttcgcggga
     1681 atgacggcgt ataagttccc gtgcggacag acctatattc ccacctgcgc gggaatgacg
     1741 attcagaagt tgcccgaaac caaaaaactg aagccgaacg gtctggattc ccgctttcgc
     1801 gggaatgacg gcatatcagt ttctgtgcgg ataaacctag attcccatct gcgtgggaat
     1861 gacgggattt tagattgcgg gtatttatcg ggaatggcgg tttggaagtt cactgaagcg
     1921 caaaaacaac ggaaacccaa aaaaccggat tcccgattgt gggaatgacg ggattttggg
     1981 tcgtgggaat ttgccgcaca cggcttcaga cggcattgcc tgccgttttg cctgcgggtg
     2041 cgactgcctt gatgtagttg agcgagacaa actgcttctc ggcatctaat tcggtgattt
     2101 tgaacaatgc ctgtgatttg ggcagcgcgt caaacggaat accggttgcg cgtgttacta
     2161 gcggcagacc ttcgatgcgg acgaggtctt ctttcagaat cgtcgccgtc agttcgctga
     2221 tgccttgctg ttgcagatag acgagactcc agtaggcttc catttggcgt tggaagtcgg
     2281 cgtaggcggt ataggcggca tcaaagtcgc gcagcgcggc gaagagttcg gcatcgctgt
     2341 tctgatacag cggctcggcg gagtcgtcga tcaggctgat cagctgcttt tggttgatgt
     2401 agtcggcggc gcggcgcagc ggcgaagtga accagccgta atgctgcacg cccatgccga
     2461 tatgcggctc ggatttggtg ctcatgcgca cttttccggt gggttggacg cggaagaggc
     2521 cgggcaggtc gttgtcatgg agcatttgtg cccaagtgct gttggcaaga atcatcatct
     2581 cgctgaccag cgtatcgatg ggcgaaccgc gttcgcggcg gacgacggat accttgcctt
     2641 cctcatccaa ttcgatgctg taatcgtatt gcggcgcgcg gtcgggttcg tatttgccgc
     2701 gtgctttttg cagggcgatg gcgaattgat agaaccaaat caagtcttga tggtgggcga
     2761 acatcatttc gcccgcttcg tccaagccgg tttcggcgtt gaaatgcggc tcgatggttt
     2821 ggatgcgtag gttttcggcg atgctgacgg cttcgatttt gcaggtcggt tcgccggtgt
     2881 tgaactcgct gtccacatca aaataaatgc tgacggcggg acggtacgcg cccgcatcaa
     2941 ggctgaacgc ggctatccag ttttcgggca gcatggtgat tttgccgccg gggaaataga
     3001 ccgtactcag gcgttccatg atgttttttt ccattttgtc gcccggttta acggcaagtg
     3061 acggcgcggc gatgtggatg ccgacgcgtt tcgtgccgtt gcccaagtcg gtcaggctca
     3121 aggcatcgtc cacttcggtg gtcgattcgt cgtcaatgga aaaggcggta acgtcggcct
     3181 tgggcaagtc gggcatttca ggaagcggaa ggtcggggaa gcttgttcct ttggggaagt
     3241 atttgatttc aaaaccgtct tgcaggtatt ggggaatgga cgtaatgccg cctgtttttt
     3301 tcgccaattc gtaggcggaa gttttcagcg cgtcggcagc tttggtaaag gctttgtagg
     3361 tcagcaactg cttgtcgggt gcgtgtaaga tggttttcaa atccgccgcg atttcagacg
     3421 gcatctcgcc gcgttttaag gcttccgccc aagcgtcgat ttgcgcgtct tgctgttttt
     3481 tgcgttcgat ggcggcaagc gcttgtttta aggtttcttc gggcgcggct ttgaacacgc
     3541 ctttggcttt tttgtagaaa tacatcggcg cggcgtaaag tgcaatcaaa gtcgccgcca
     3601 gctcggtttt ggtcggcgcg tgtccgtaat attcttcggc gatggcttcg gcggtaaatt
     3661 cttcttcgcc gcatacctcc cacaataaat cggtgtcgat gtccgccgcc tgcgcctgcg
     3721 cgttctccaa aaacgccgcc atatcgccgt cgaactcggt aaagacgttg ttcgccttca
     3781 ctttggtgcg tttgccgtgt ggggtatcga cttggtaggt ggcatcgttt ttttggatga
     3841 tggcggcgat tttgaattgg ccggactctt cgtaaaaaat attcattttt cggatttttc
     3901 tgtggaaact caagcgggcg attttagcag attaccgaaa atgccgtctg aaaaaaggtt
     3961 gggagagggt tggcgcgtct ttgcggtgct tgcg
//
LOCUS       DAXHSJ010000163         3992 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000163 DAXHSJ010000000
VERSION     DAXHSJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.163"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            148..1665
                     /locus_tag="ACFM8U_001821"
     CDS             148..1665
                     /locus_tag="ACFM8U_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6335011.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALSQVWRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSLAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1724..3505)
                     /locus_tag="ACFM8U_001822"
     CDS             complement(1724..3505)
                     /locus_tag="ACFM8U_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC6335012.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYGFGLPLMGVDSERGGNLAMHWFADCADIDAASWLAQHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVKLYQRLRQPVPYAV
                     LSHLPDAQGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENRQCALLDRHLCRLKTSAQALNLPLPDGCENQIKQYIAHLPDGVFRVKARLASDG
                     ISLSRAVLNRLTDKQRVIISPAVLPAQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKYQGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          842 a          886 c         1301 g          963 t
ORIGIN      
        1 gtgaaatccg ccctttgaaa atcgggcggc ggttttttgc ctgcccgctt tcaggcggca
       61 aagccggttt ttacgggctt ttgcctgttt ttcggatggt ttgacgtgct tcggcggcgt
      121 gtttgccgga aaggtaaatg acagggtatg ttgtatttca gatacggttt tttggttgtt
      181 tggtgtgcgg caggtgtttc tgccgcctat ggggcggatg cgcctgcaat tttggatgac
      241 aaagctttgt cgcaggtgtg gcggtcggtg tcggataaat gggcggaatc ggattggaaa
      301 gttgaaaatg atgccccgcg cgtggttgac ggggattttt tgttggcgca cccgaaaatg
      361 ttggaacata gtttgcgcga cgcgctcaac ggcaatcagg cggatttgat cgcttcgttg
      421 gcggatttgt atgccaagct gccggattat gacgcggttt tgtacggcag ggcgcgggct
      481 ttgctggcga aattagcggg aaggccggcg gaggcggtgg cgcggtatcg ggaactgcac
      541 ggggaaaatg cggcagacga gcggattttg ctggatttgg cggcggcgga gtttgacgat
      601 ttccgactga agtcggcaga aaggcatttt gcggaggcgg caaaattgga tttgccggcg
      661 ccggttttgg aaaatgtggg gcgttttcgg aaaaaaacgg aggggctgac gggctggcgt
      721 ttttcgggcg gcatcagccc ggcggtcaat agaaatgcca ataatgccgc gccgcagtat
      781 tgccggcaaa acggaggccg gcagatatgc agtgtcagcc gggcggagcg ggcggcaggg
      841 ttgaattatg aaatcgaggc ggaaaaactg acggcgttgg cagataatca ttatttgttg
      901 ttccgttcca atatcggcgg cacgagctat tatttcagta aaaaatcagc ttatgatgac
      961 gggttcggca gggcgtattt gggttggcag tataaaaatg cacggcagac ggcggggatt
     1021 ttgccgtttt atcaggtgca gttgtcgggc agcgacggct ttgatgcgaa aacaaaacgg
     1081 gtaaacaacc gccgcctgcc gccgtatatg ctggcgcacg gagtcggcgt gcagctgtcc
     1141 catacttacc gcccaaaccc gggatggcaa ttttcggtcg cgctggaaca ttaccgccaa
     1201 cgctaccgcg aacaggatag ggcggaatac aataacggca ggcaggacgg gttttatgtt
     1261 tcgttggcaa aacgtttggg tgaatcggca actgtgttcg gcggctggca gtttgtgcgg
     1321 tttgtgccga aacgtgaaac ggtgggcggc gcggtcaata atgccgccta ccggcgcaac
     1381 ggtgtttatg ccggctgggc gcaggagtgg cggcagttgg gcggtttgaa cagtcgggtt
     1441 tccgcgtctt atgcccgccg caactataag ggcgttgcgg ctttctcgac agaggcgcaa
     1501 cgcaaccgcg aatggaatgt ctcgctggct ttgagccacg acaagttgtc gtacaaaggt
     1561 atcgtgccgg cgttgaatta tcgtttcggc aggacggaaa gtaatgtgcc gtatgcgaaa
     1621 cgccgcaaca gcgaggtgtt tgtgtcggcg gattggcggt tttgaatggt gggataatgc
     1681 cgtccgaact ttggaaacag gttcggacgg catttttgcg cgttcaggca agggcggcgg
     1741 caaatacgcc gcgcagggcg ttggagaggc ggatttcttc ggcttcttgc agtgtttttt
     1801 gtgtgatgtg tgtttcgatt acttgatttg tttgcaaata tttttgcggt tcgtccaata
     1861 cggcttggcg cattatgccg tttaaaatgt ctaaatctaa agagggcgta agccattgtc
     1921 cttggtattt gacgaacacg ttgcttctgc cgccttcgag caggatgccg tctgaattga
     1981 aaaacaggct gtcgaacgcg ccttgtgttt cggcggtttg ccacgcttgg tcgaagacgg
     2041 tgcggcaggt ggttttgaag cggcgcaggt agttttgtgc gggcaggacg gcgggcgaaa
     2101 tgatgacgcg ttgtttgtcg gtcagacggt ttaaaacggc gcgggacagg ctgatgccgt
     2161 ctgaagcgag ccgggctttg acgcggaaca cgccgtcggg caaatgggcg atgtattgtt
     2221 tgatttgatt ttcgcaaccg tcgggcaggg gcaggttgag ggcttgggcg gaggttttca
     2281 gacggcatag gtggcggtcg agcagggcgc attgcctgtt ttccacgcgc agggtttcaa
     2341 aaatgccgaa gtcggggcgc aattcgttga ggaaacgggc tttccagccg cattcccgat
     2401 attcggcggc agggtcgctg tcgatgacga tgccggaacc gacaccgtac acgccttgat
     2461 aaatgccgtc tgaaagcggc gtgagcgaca aggtgcggat aacgacgttg aacgtgcctt
     2521 caaaccccaa gccgccggaa cacgggttca aatagccgat gctgcccgta taaagtccgc
     2581 gcgcttcggc ttcgagcgat tcgataatct gcatactcat ttttttgggc gcgccggtga
     2641 tgctgccgca ggggaaggcg gcgcggagga tgtcggcgaa cgaggtgtgc ggcaaggctt
     2701 gggcttggat ggtgctggtc atctgccaaa cgctgccgaa acgcgatact ttaaacggtt
     2761 cgggtacgca tactgtgccg gtttgggcga ttttgccgag atcgttacgc agcaaatcga
     2821 caatcatcac gttttcggcg cggtttttcg ggtctgcttg caactcggcg gcgcggcgtt
     2881 cgtcttgtcc gtcgcccaaa atcggcgcgg tgcctttcat cggttcggtg ctgatggtgc
     2941 cgtccgaacc gattttgagg aagagttcgg gcgagaaaca cagcgtccac gcggattgcc
     3001 cttgtgcatc gggcaggtgg gacaaaacgg cataggggac gggctggcgc aggcgttggt
     3061 aaagtttgac gggattgccg taggcttgca ggtgcaggcg ggtagtgtag ttgatttgat
     3121 aggtgtcgcc gcgtcggatg gcttcgtgga tttggcggat atggtcgagg taatcggctt
     3181 cggatacgga ggattgcggc gtggaaatgc cggcggggag gccgtctgaa tgttgggcaa
     3241 gccagcttgc ggcatcgatg tcggcgcagt cggcaaacca gtgcatggca agattgccgc
     3301 cgcgttcgga atcaaccccc atcagcggca aaccgaatcc gtagtctgca aacaacaccg
     3361 aatgcagccc tttttgccag cccgattgca gcgcgccgtc caaagcatcg agttcttcgg
     3421 gacggaaaaa acggctttct acatgatttt gatagcgttt tgcgcgaccg cttacggcat
     3481 cgtcaaacag ggcgaaatag ggcatggcaa ttcggggcaa atgttttgat tatacgctct
     3541 ttttacacat attttcagac gattcggcaa atatcggcaa aatgtaattt tatgtagaga
     3601 aagtgggggg cgagggtgta aaattgtgaa aacggatttt catcgtattt ccttttcggt
     3661 ttaaaccccg ccctttaggg cggtagaatc agactttatt tgggaggggc gtaacccctt
     3721 ccaaatcagg gcaacacata gggcgacgct ttatgtgtcg tcctgtgtgt tgaaacattt
     3781 ccaacctgaa atacaataag gagaccttta tggcagacca ccagctgcaa ccgtttgaaa
     3841 acgtagaatt aggcgaaaag caagatcagc tccaagtata gtggattaac aaaaaccagt
     3901 acagcgttgc ctcgccttgg ctcaaagaga acgattctct aaggtgctga agcaccaagt
     3961 gaatcggttc cgtactatct gtactgtctg cg
//
LOCUS       DAXHSJ010000164         3872 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000164 DAXHSJ010000000
VERSION     DAXHSJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.164"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="ACFM8U_001823"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="ACFM8U_001823"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC6335013.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="ACFM8U_001824"
     CDS             1439..1924
                     /locus_tag="ACFM8U_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC6335014.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFM8U_001825"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFM8U_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC6335015.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGYGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDGLSFKVPAGAIVGIIGPNGAGKSTLFKMISGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIAGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACESDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          796 a          944 c         1075 g         1057 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cctcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 tcgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataag
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgccga tgtcaaacct
      661 gaggacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggcgcg
      721 ataccgcgtt gggtgttgtt aagtgcggct ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccat ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacgg ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggcg tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgcgcctt cttcgcccaa
     2041 acggcgtttc ttatcggctt cgtattcctg atagttgccg tcgaagaaca cccatttgga
     2101 gtcgctttcg caagccaaga tatgcgtagc aatacggtcg aggaaccaac ggtcgtgcga
     2161 aatgaccatc acgctgccgg caaattccaa caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcaagg tcgttagacg gttcatccag cagcaacaca ttgccgccgc ccaacaaggt
     2281 ttttgccaag tgcagacgac cgcgttcgcc gccagacaat tgacctgcaa ttttgctttg
     2341 gtcgctgcct ttgaagttga agcgtcccaa atattggcgg gcgggaattt caaactgacc
     2401 aacctgcaaa atatcgcggc cttcggcgat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaggctcat tttcacggtt tgtccgattt tcacctcgcc
     2521 ggaatcaggc tgctctttgc ccgaaatcat tttgaacagt gtcgatttac ccgcgccgtt
     2581 cgggccgatg atgccgacaa tcgcgcccgc aggtactttg aagctcaaac cgtcaatcag
     2641 cactttatcg ccaaacgatt tggaaacatt cacaaattca atcacttcat tacccaaacg
     2701 ctcggcaaca gggataaaga tttcctgcgt ttcattgcgt ttttggtatt catagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatagccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgcgttacc gcaacgactg tgccggggaa gcgcacgaga aattgctcca gccattccac
     3061 cgattccgca tccaagtggt tggttggctc gtccagcagc agcatatcgg gcttgctcaa
     3121 caagagtttg cacaaagcga cgcggcgttt ttcaccgccg gacagattgc cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc agcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcctgtggac gaacccgccg caataattgc ttccaaacgg ccctgttctt ccgccaacgc
     3301 gtcaaaatcc gcatcgggat tggcgtactc ggcatacact tcttccaagc gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcacgc acggtttttt ccggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacaccag ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcaaa ccgattttcg cgccggggaa gaaggaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 agttttctcg tttcggtcgg acaaatttca gacggcacat tttaaccgat aatttgattt
     3721 aagccagttt atccgcgaac cggtattgcc aaaatcgggc aggattcata aaatccgtct
     3781 atccctttga aattatacaa acaaaaaaaa taataatgat aggtgatcgc cgcctcggca
     3841 accattttgg attttccaaa gcaaatatag tg
//
LOCUS       DAXHSJ010000165         3866 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000165 DAXHSJ010000000
VERSION     DAXHSJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.165"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            1..890
                     /locus_tag="ACFM8U_001826"
                     /pseudo
     CDS             1..890
                     /locus_tag="ACFM8U_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            895..2097
                     /locus_tag="ACFM8U_001827"
                     /pseudo
     CDS             895..2097
                     /locus_tag="ACFM8U_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            2221..3771
                     /locus_tag="ACFM8U_001828"
     CDS             2221..3771
                     /locus_tag="ACFM8U_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC6335016.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGNEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEA
                     GMVSEPQQ"
BASE COUNT         1074 a          978 c          986 g          828 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcgccgatt accgtcgtca actacgacga acaagccctc
       61 aacaacaccg aggcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gcgaaagcaa cacgctgacc ggattgtatt tcagaggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggtttgaca ggtatgtacg gcacgcaagg cacgaccagc
      241 gtgcaagtcg gctccgcaca actgattcaa ggcgcgtcca ccgccgtaaa cggcatgaac
      301 cctgatggcg cggtatccgg ttccgtcaat atcgaaacca aaaaagccgc tgatgaaggc
      361 aaccgcaaaa tcggtttggc ccggttcggc aacaaccgcg cccaaggtac gttcgacttg
      421 ggccaacgtt tcggcgaaaa caaagcattc ggcgtgcgcg ccaacggcaa actgcgccac
      481 ggcgacaccc cgcgccacgg ttacagcgaa gacaacaaag aatttgcggt aaatgccgac
      541 tatcgcggcg aaacactgcg cgtagcgttc gattccatct acgcaaaacg caaaaccaac
      601 ggcggtcgcg cgcgtatgca ggacattcaa aacgccggcg gacgcttgtc ctgactttgg
      661 gcggacgttt ccaagcggtt gaatacacca ataagaaaaa atcacgcgga acggcagccg
      721 gagctagcaa ggtagaagtg aaggcaaacg gaaatattga gcatcaattg gcatcgttta
      781 cagccgacta tcccaattct tgggagaact ctgccaactt ggatgccaat atcaaagtca
      841 acagcctggc tttgtccgac accttgggct tcgtggataa caaataccgt tggcgcgccc
      901 gatggtaaaa ccaacctgct gccttcttgg aactggcaaa ataccgccgg ccaaaccaat
      961 atgcttacgt ttgaatggga tgcgtttgac aatgcccaaa ttaccggcgg tatcggctac
     1021 aacaaggcgc gttattacgg cacgctgatt tccccgaccg tttgcggtac aagcggtgcg
     1081 agcgaccaaa ccgctacctg cacaagtgcc aagcaatacc acaccggcac ggcgagcctg
     1141 accgaccaat atttccgcac attgagtatg aacctgaccg cacgcggcga atttgaaacc
     1201 ggtccggtaa cacataactg gagcaccgcg ttcgaccgtg ttatccgcca gcggaaaacc
     1261 attcagagcg gcgatgccaa acgtttcagc ccgatgctga tggcggcgtg ggtgccgcaa
     1321 cctgatttgg ttgtgtacgg caactatatg gaagacttgg agcctgccga tatcaaaacc
     1381 gatgattccg gcgaaacgac aatggcgaaa ccgcgtgtca gccgtcagtt tgaagtgggc
     1441 gtgcgtaaaa actggggcaa tttcgtaacc acgttaaacg cgttccaaat caaacgcccg
     1501 ggctactggc gcggtcatac aactaccaag acaacaaaag gggtaacaac aacgctgacc
     1561 tatggcaaca attccgattt cgcccgctat aaggcgcaag gcggcgtagc cggcgacgag
     1621 caaggtatgg aacgcaatcg cggtatcgag ttcaatgcct atgctaacct gttaaacaaa
     1681 accctgcgtc cgactttggg cctgatgtat ctgcaatcga ccgtgaaaga ctacccaaat
     1741 tcacgcgata tgctggttaa cggcgtacaa gtcgccaatc cgcgcgtaat tgccaaggca
     1801 ggtttggaat gggatacacc gtttgccaaa ggcttgactt taaacagcaa tgtttcgtat
     1861 ttcggcaagt cttaccaaga cacgcaaaaa caatacgcct tcccatccta taccttggtt
     1921 gatgtaggcg cgcgctacaa aaccaagctc ggcaaaaata cgttgaccgt cagcagctcg
     1981 gtggaaaatc tgttcaacaa aaactactgg caggtacagc gcggccaata cgaccgcagc
     2041 ttcgccgtcg tcggtctgcc gcgtacttac tggctgaagg cggaattgga tttctgattt
     2101 ctccgcatct ggaaaatgcc gtccgaacag gtttcagacg gcatttcttt taaagagata
     2161 tggcgggata cgcttaaata atgctattct agcgtctttg aaacctgctt gtttgaagtg
     2221 atgaaaaaac ctttgatttc ggttgcggca gcattgctcg gcgttgcttt gggcacgcct
     2281 tattatttgg gtgtcaaagc cgaagaaagc ttgacgcagc agcaaaaaat attgcaggaa
     2341 gcgggcttct tgaccgtcga atcgcaccaa tatgagcgcg gctggtttac ctctacggaa
     2401 acgacggtca tccgcttgaa accggagttg ctgaataatg cccggaaata cctgccggac
     2461 aacctgaaaa cagtgttgga acagccggtt acgctggtaa accatatcac gcacggccct
     2521 ttcgccggcg gattcggcac gcaggcgtac attgaaaccg agttcaaata cgcgcctgaa
     2581 acggaaaaag ttctggaacg cttttttggg aaacaagctc cggtttccct tgccaatacc
     2641 gtttatttta acggcagcgg taaaatggaa gtcagtgttc ccgccttcga ttatgaagag
     2701 ctgtcgggca tcaggctgca ctgggaagga ttgacgggaa aaacggttta tcaaaaaggt
     2761 ttcaaaagct accgcaacag ctatgatgcg cccttgttca aaatcaagct ggcagacaaa
     2821 ggcgatgccg cgtttgaaaa agcgcatttc gattcggaaa cttcagacgg catcaatccg
     2881 cttgctttgg gcagcagcaa tctgaccttg gaaaaattct ccctagaatg gaaagagggt
     2941 gtcgattaca acgtcaagtt aaacgaactg gtcaatcttg ttaccgattt gcagattggc
     3001 gcgtttatca atcccaacgg cagcatcgca ccttccaaaa tcgaagtcgg caaactggct
     3061 ttttcaacca agaccgggga atcaggctca tttatcaaca gtgaagggca gttccgtttt
     3121 gacacgctgg tgtacggcaa tgaaaaatac ggcccgctgg acatccatat cgccgccgaa
     3181 catctcgatg cttctgcctt aaccgtattg aaacgtaagt ttgcacaaat ttccgccaaa
     3241 aaaatgaccg aggaacaaat ccgcaatgat ttgattgccg ccgtcaaagg cgaggcttcc
     3301 ggactgttca ccaacaatcc cgtattggac attaaaactt ttcgtttcac acagccgtcg
     3361 ggaaaaatcg atgtgggtgg aaaaatcatg tttaaagaca tgaagaagga agatttgaac
     3421 caattgggtt tgatgctgaa gaaaaccgaa gccgacatca gaatgagtat tccccaaaaa
     3481 atgctggaag acttggcggt cagtcaggca ggcaatattt tcagcgtcaa tgccgaagat
     3541 gaggcggaag gcagggcaag tcttgacgac atcaacgaga ccttgcgcct gatggtggac
     3601 agtacggttc agagtatggc aagggaaaaa tatctgactt tgaacggcga ccagattgat
     3661 actgccattt ctctgaaaaa caatcagttg aaactgaacg gtaaaacgct gcaaaacgaa
     3721 ccggagccgg attttgatga agccggtatg gtttcagagc cgcagcagta atgggtcaaa
     3781 tgccgtctga aatgttcagg tggcattttg ttgtattgga aactgccgtt ttcaaccatt
     3841 ccttcagata agccgtgtcg gattcg
//
LOCUS       DAXHSJ010000166         3783 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000166 DAXHSJ010000000
VERSION     DAXHSJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.166"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(316..2748)
                     /gene="hupB"
                     /locus_tag="ACFM8U_001829"
     CDS             complement(316..2748)
                     /gene="hupB"
                     /locus_tag="ACFM8U_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC6335017.1"
                     /translation="MHIPFKPVLAAAAIAQAFPAFAADPAPQSTQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDIEIKNDGYVYNPTDTGGPSKYLTYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFVKLYDPKILTSNQAKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAETAK
                     NFELGLAGSGKAGNFKFSGFQTRYRNFIELTYMGVSSDKPNSPNYAPISDGTALVSSP
                     VWQNQNRSSAWVKGLEFNGTWNLDSIGLPQGTHAGVNVSYIKGKAKQTNGQETPINAL
                     SPWSAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(2779..>3783)
                     /gene="hupA"
                     /locus_tag="ACFM8U_001830"
     CDS             complement(2779..>3783)
                     /gene="hupA"
                     /locus_tag="ACFM8U_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
                     /protein_id="HFC6335018.1"
                     /translation="PHVPVSIPTATPLPKGEVTLSSDNGNIENINTANTETHPPRRTR
                     RSLYASPQNTSSGISIQQREVEKIYFGVKSPEKSFIFQTPGGAQYALSSYADPIVPSY
                     SSPDFKIPDRHAGQRLADGSRIFICCSDSGATNQAEITKQDYMKFGAWIGPNGEIDLF
                     AGGFPVGKTPASSSYYSSSTLETAGKGKITYQVWGIRVRNGQFVTSSYTPPKNSGYYS
                     STPTNTPVLSFITANFNSNKLAGEIRGNSDYGPSVKIENATINQLSFSGTATSGGKTG
                     NLEGKFFGKFNGYRDTETSIGGKITFDGDRSLDTVFGGVSYKKELENNTDMSTTHLTK
                     "
BASE COUNT          716 a          852 c         1193 g         1022 t
ORIGIN      
        1 gggaatgacg tggtgcaggt ttctgtgcgg atagcttcgt cattcccgcg caggcgggaa
       61 tccaggttcg ttgagtttca gtcatttccg ataaattctt gcagctttgc gtttctagat
      121 tcccacttcc gtgggaatga cggagagtcg cgggaatgac ggagagttgc gggaatgacg
      181 gagagttgcg ggaatgacgg agagttgcgg gattgggctt ggtacaagcc tgaaaggggg
      241 cggcggtgcg accgtaaaaa atgccgtctg aaacccaaat catcgggttt cagacggcat
      301 tggcacatca ggagcttaga acttcgcttc gatggtgaaa ttgtagctcc tgcccggcga
      361 ggtaaagcgt tggatgccgg cgtgggtttt gttgtcaacg cggttgactg tgccgaattc
      421 gcggatgctg cgtaaagatt cccaagtgta gtactgcttg ttggtaatgt tgtacgcggc
      481 ggcgcggagc gtaacctgtt tgccgatgtt gaggtaggcg gaaaggtcga acagcgtata
      541 ggccttgctg tgtttggcat aaggccacgg gttgttcaaa tcgtcgttgc tgtggacggt
      601 gtcggacggc tttttggcgg cggtgcgcgt ggcgtaggcg ttgatgcccc agcgtttgga
      661 aggcgcgtca tagcccaggc tgtaaaccgc actccatggc gaaagggcgt taataggggt
      721 ttcctgtccg ttggtttgtt tggccttgcc tttgatgtag ctgacattga cacctgcgtg
      781 cgtgccttgc ggcaaaccga tgctgtcgag gttccatgtg ccgttaaatt ccaaaccttt
      841 cacccatgcc gaggagcggt tttggttttg ccaaacgggc gagctgacca atgcagtacc
      901 gtcggaaatc ggcgcataat tagggctgtt aggcttgtct gaggatacgc ccatgtaggt
      961 cagttcgata aaattacggt agcgggtttg gaaaccggag aatttaaagt ttcctgcttt
     1021 gccgctgccg gcaagtccca gctcgaagtt tttggcggtt tctgccttca ggttggggtt
     1081 tgctttaagg taaaagtcag gatgcgggaa caatagccag gtttcgtccg aagtcggtgc
     1141 gcggaagccg gtgctgtatt ttgccaacaa gtgcagatgc ttggtaaacc gccagtcgaa
     1201 ccccgtgccg tagctgaagc ccgcgtgcgc gcgttccgaa cccaaatggg gaatctgccc
     1261 ccggatggcg gtggtgtatt tcggatcgtc tttcgcgctg ctgctgtttt tgtcgtagcg
     1321 tatgcccgca ttcaggcgga agcggtcgtt gccgcccaag tgaaacgcat tgttccaata
     1381 ggcaaattta tatttcgacc ggttttcgat cagcattgtg attttggctt ggttggaagt
     1441 gaggattttg gggtcgtaaa gttttacgaa atagctgtaa tccgagttgg cattgtcccc
     1501 tctgccgccg cccaagccgt attgcgccgc ccaaacggct ttggaaaagt cgagttgttt
     1561 ttcaaaatcg gcagtccatt gcgcggtgtt ttggcggata tgccggaacg aatggtaaac
     1621 ctcgccgttg atgccgcgca aatcgtaatt tttcgggatg tcccaagtcc aagtgttcat
     1681 atcgatgcgc tgcttgtcgt agcgcagctt gaggctgtcc cacgggccgt gttccaattc
     1741 gtttttgtat tcgacaccgg agcggcggcg gtagctcacg tcttggcggt ggcggtagtc
     1801 gcccgttgcg gcagacgtgg tcgtacccgt ccacaaatta gacagctcgt tggtaaaacg
     1861 gtcggtgcgc gagtcttcaa aaatccagcc gatacggttt tgatcgttga agttgtagcc
     1921 cagcttgaac agggtgcttt tgtttaccca ttcttgcggg tcgggttggg agcgcgcaac
     1981 ccctgtggct acataggtca ggtacttgct gggtccgcct gtatcggtcg ggttatagac
     2041 atatccgtcg tttttaattt cgatatcgcc ctcggtcgag cggtttttgg tttctttgcc
     2101 gaagcggcgg gtataaacca ataaggcatc caaaccaaag agcctgccgg cggcggtgat
     2161 gctgctgaat ttttggctgt ttttgccgac gctgccgccc tttatcccca aatggtaggg
     2221 cttgtcttcg gaaacataat cgcttgcgga tttggtttgg taattgactg cgccgcccaa
     2281 tgcgccgctg ccggatttga gcgagtccgc gcctttggtg atggttactt cggaaaagtt
     2341 ttccggctcg gaagtgttgc ggttggcgtt gaagttgccg tacgcgccga acaattcttg
     2401 gaaggcttcg gaagagcggc tttccgcctg cgccagcccg tcaacgttga tggcgacgcg
     2461 gtctttgtcc acgccgcgta tggtaaagcc gttggaaccc gccctgccgc cttcgacgac
     2521 ggaaatgccg gggtcgtagc gcaccaggtc gtgttcgtcg ttgaccaaga gcttgtctaa
     2581 ggttttgcgg cggatttttt cttcgccgag tttttgggtt ttgtgcgtgc cggtaacggt
     2641 aatttcgttc agcgtttggg tggactgcgg cgcggggtct gccgcaaagg cgggaaacgc
     2701 ttgggcgatg gcggcggcag ccaatacggg cttgaaaggg atgtgcataa gaaaaccttt
     2761 ttaaaagcgg attaattatt attttgtgag atgggtggta ctcatatcag tgttgttttc
     2821 aagttctttt ttataactca caccgccgaa tacggtatcg agggagcggt cgccgtcaaa
     2881 ggtgattttg ccgcctatgc tggtttcagt gtcccggtag ccgttgaatt ttccaaaaaa
     2941 cttgccttcc aagttgccgg ttttgccgcc ggaggtggca gtgccggaaa aggataattg
     3001 gttaatcgtt gcgttttcaa ttttcacact cggcccgtaa tcgctgttgc cgcggatttc
     3061 gcctgccagt ttgttgctgt tgaaattggc ggtgataaag gaaaggacgg gggtgttggt
     3121 aggtgtggac gaataataac cactgttttt cggcggcgta taagaggaag taacaaattg
     3181 cccgtttctg acgcggatgc cccaaacctg ataagtgatt ttgcccttgc cggcggtttc
     3241 gagcgtgctg ctgctgtaat aactgcttga tgccggtgtt ttgccgacgg ggaagccgcc
     3301 ggcgaagagg tcgatttcgc cgttgggacc tatccacgcc ccgaatttca tataatcttg
     3361 tttggtaatt tccgcttgat tggttgcccc ggagtcgctg cagcagataa agatgcggct
     3421 gccgtcggca agccgctgcc ctgcgtggcg gtcgggaatt ttgaaatcgg gggaggagta
     3481 gctggggacg atggggtcgg cgtaggatga aagggcgtat tgcgcgccgc cgggtgtttg
     3541 gaagatgaat gatttttctg gtgatttaac accgaaatag attttttcta cttcccgctg
     3601 ctgaatggaa ataccggagg atgtgttttg cggggaggca tagagggagc gtctggtgcg
     3661 gcgcggcgga tgtgtttcag tgttggcggt gttgatgttt tcgatattgc cgttatcgct
     3721 tgataacgtt acctcgcctt tgggcagcgg cgtggcggtg gggatggaca cggggacgtg
     3781 cgg
//
LOCUS       DAXHSJ010000167         3740 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000167 DAXHSJ010000000
VERSION     DAXHSJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.167"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            159..496
                     /locus_tag="ACFM8U_001831"
                     /pseudo
     CDS             159..496
                     /locus_tag="ACFM8U_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
     gene            510..683
                     /locus_tag="ACFM8U_001832"
     CDS             510..683
                     /locus_tag="ACFM8U_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335019.1"
                     /translation="MSKKSLIALMTAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKKRHK"
     gene            608..940
                     /locus_tag="ACFM8U_001833"
                     /pseudo
     CDS             608..940
                     /locus_tag="ACFM8U_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            937..1347
                     /locus_tag="ACFM8U_001834"
     CDS             937..1347
                     /locus_tag="ACFM8U_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HFC6335020.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKTQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEMRQKERNSSQG"
     gene            1352..1900
                     /locus_tag="ACFM8U_001835"
                     /pseudo
     CDS             1352..1900
                     /locus_tag="ACFM8U_001835"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1916..2101
                     /locus_tag="ACFM8U_001836"
                     /pseudo
     CDS             1916..2101
                     /locus_tag="ACFM8U_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2420..2782)
                     /gene="ssrA"
                     /locus_tag="ACFM8U_001837"
     tmRNA           complement(2420..2782)
                     /gene="ssrA"
                     /locus_tag="ACFM8U_001837"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2845..3450)
                     /locus_tag="ACFM8U_001838"
     CDS             complement(2845..3450)
                     /locus_tag="ACFM8U_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC6335021.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT          937 a          843 c          993 g          967 t
ORIGIN      
        1 gctgccaatc aatcacttga tccaacttca atagcgggaa acggtcgatg tgtttggcaa
       61 tcatggcttg ggcggtttgc tggaagaagg tgctcatgag aaatctccta aatgtcttgg
      121 tgggaattta ggggattttg gggaattttg caaaggtctc aatcagcatt taccgtaaag
      181 cgttttggga gcgttaccgc gccgaccaaa tgccggaagc ggtccgttcc aattttttga
      241 tgcctgcgtc aaccacggtt acggcaatgc cgcccgtatg ctgcaacgcg ccgcaggcgt
      301 accggacgac ggcgttatcg gagcagtcag cctcaaagcc atcaattccc ttcccgaaaa
      361 cgacctttta ttgcggttca acgccgagcg tctggtcttt tataccaagc tcggtacgtt
      421 cacctctttc ggcaagggct gggtacgccg tgtggcgcaa aacctgattc acgcgtctgc
      481 agataacact gattaaaggg agataaacca tgtcaaaaaa gtcactcatc gccctaatga
      541 ccgcagccat gcagcccgat ttcagccaca gcgacctagg cattcgctac gccatgccga
      601 ctcagggatg ttggacgcaa gcccaccgca agagcggggt agccgccgcg aaacgcgcag
      661 ccaaaaaaaa gcgtcacaaa taaccgcctt tttccgatgg ctgggcggct tggtctctaa
      721 tccggccaca ggaaaaatca gccataccaa actatgggca aacgtcgccg cagccgccat
      781 gacttggaag ttcgtgcagg cggcggacgc gcccgaatgg ctctagtggg cttatggcgc
      841 attggtcggc gggtatgcat taatcaaacg cggcatcgcg gcgattccgc agttggcaga
      901 aatcaaaaaa tccgcaaatc aggaaggggg gcggcaatga ttgaatttgt ccgagccaaa
      961 aaacggctgc tttgggcatt tgtgcttttg cttgtgtgga cgtgcggtta ccgatacgcc
     1021 gccgacaagg ccgaagcgga acaaaccgcc ctgattgcca cctatcggca ttcttctatg
     1081 gttgcggcgg aacaatatgc cttgcagctt aaaaaaacgc aggacgaaag gcagcggtgg
     1141 tacgactttt cccaaaaaca aggaagaaag cccgtgaaaa aacagtatcc gccgcaaacg
     1201 aaaaaagccg gctatctgaa aaccaaggaa gaactgcttg cggaattggc ttgccttaaa
     1261 gcggaaatgg ttgccctaaa aaagcccgat gccttaatcc atgggaaaga aatgcggcag
     1321 aaagaacgca actcgtcgca gggttaaggc aatgccatcc gttgaactgc tgttggagat
     1381 tgtcggactg ccacgcggca ccttttatta ccaattggcc gtccaatcgg cagaagacaa
     1441 atatgccgat ttgaaacagc atatccatga tatttatcga cgacataagg gaagatacgg
     1501 ctaccggagg attgcagcag ccatccttca tgcaggaaca ccggtcaatc acaagaaagt
     1561 cagccgtctg atggcgaaga cggggctgaa ggcagtgata cggcggcgca aataccgctc
     1621 gttcaaagga gaagtcggca aaattgcgcc gaatatcctg cgacgctgtt tccatgcaga
     1681 aaagccgaat gagaaatggg taacggacgt taccgagttc aatgtaggcg gagaaaagat
     1741 atacctttct ccgattatgg atttgtttaa cggggaaatc gtcagttacc gtattcaaac
     1801 ccgcccgact ttcgatttgg ccgacgagat actgaaaggt gcgccggaga aaccgggatc
     1861 gtctgaaaag ccgatactgc attcggatca aggttggcaa tatcagatgt ttttttatag
     1921 tggattaaat ttaaatcagg acaaggcgat gaagccgcag acagtacaaa tagtacggca
     1981 aggcgaggca acgccgtact ggtttaaatt taatccacta tacaacaaag atagaatcaa
     2041 gttgaaatta aaagggctga gccttgttca gtacagaatt cagtccctga aagccgcttg
     2101 attaaactgt ccgacttttt ggggtcagtt cggcttcggc atttttttat ccgtttgggg
     2161 gtaacttgtt tggaaagctg caagcttata aataaaggat tacatttaag ttttgggtaa
     2221 cctttttaaa aaaatgcgtg atgacttttg catttttaag gcgttttttg gggtaattcg
     2281 tgaaaagtta ccccaaaagt taccccataa atggcgaaaa ctcaagcata cgccagcata
     2341 ttcaaggcaa caaaaaagcc ttgaaactgt tgaagttcaa ggcttttttg tgttgcagga
     2401 tactgctgaa aatagggtat ggtggaggcg gggggaatcg aacccccgtc cgaaagtcct
     2461 ctacaaagcg ttctacatac ttagttgtgt ctatttgaaa atcttatttc catcatgccg
     2521 accaacaggc cttttggaaa ccagttacct taaatctcat ttcctgccaa gtaacccggt
     2581 aggaaaccag tcaatgtaag atgacgttgc ggtggctttc gccacacagc ccattgaccg
     2641 actgctgcaa cggctagcct taagcggcta aagcgtaagt ttcgtcgttt gcgactattt
     2701 gaattcagtg ttttacggga atctgagacc ccggtatgcc cgcatctgct tcgcaaccct
     2761 cgtcgaaacc aaggtcgccc ccagaaatgg tttgcaaatt atacggatat tgtgcggtgc
     2821 tgccaagtct gtcggagaaa tttgtcagtc ttgctgcctt aatttgcgtt tgagcaggat
     2881 gcggacgcag ccgtcgttgc cttcccgggg ttcggcgtag gcgagtacgt cggggtgttg
     2941 catcagccag tttcgggtca tatttttcag aacgggtttg tagcctttgg aacctaatcc
     3001 gctgccgtgg atgatttcgc cgcatacgcc gcgtttttgg gtgaatgcga tgaattcgtt
     3061 gaggactttt tgggcttctt cctgtgtgta gccgtgcagg tcgacatcgg taacgacggg
     3121 atagtatccg tttttcaggc gttggatgtc gttttttccc tgtccgtttt tgctgaagct
     3181 ggcgggcggg tcgttgtatg tgctgccgat gtagaagtag ttttcttcgt cggcgcggtt
     3241 gtctttggga cggactttga tgggggtttt gtcgggcggc gcataatatt gctgccggtt
     3301 ttttaatggg gagacttgtc cgactgcttg tgaaaaatcg aaatcctgtt cttgtttttg
     3361 cttgtttttt tcggctgcct gtttttctgc cgcttctttt tgggcttgtt tgcccagttg
     3421 tttgaggatg ttttggaagt cggtattcat attttttcct gttatttgtc cgatggctgt
     3481 ttcgggcggg gttttaattt gccggaatgt ttaccaatcg ggggaggatg attttgttgc
     3541 ctgcgtatgt tttttgaaag tgtgattgta tatcaaaaag aaatgcggca accgtcggca
     3601 gtgttgattg ccggaaatgc ggaccggtcg aaccgatatg cccgaacgcc tgataaagtt
     3661 ttaaaaacct gccttgcgaa gcaggctgac gtgttttgcc aatcttgaat tgccggaaac
     3721 gcgaaacacg gaaatctgat
//
LOCUS       DAXHSJ010000168         3734 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000168 DAXHSJ010000000
VERSION     DAXHSJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.168"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            299..1420
                     /gene="dnaJ"
                     /locus_tag="ACFM8U_001839"
     CDS             299..1420
                     /gene="dnaJ"
                     /locus_tag="ACFM8U_001839"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC6335022.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1616..3634
                     /locus_tag="ACFM8U_001840"
     CDS             1616..3634
                     /locus_tag="ACFM8U_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC6335023.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDIEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLMADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNADRIGTLFAAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMIIFARRVLKAGR"
BASE COUNT          801 a          839 c         1110 g          984 t
ORIGIN      
        1 aaaccagtgc tgcccgcgga taaaaaataa aataggggga agtctgcagc cgcatcaaat
       61 gccgtctgaa catgcgttcg ggcggcgttt ttataacaaa aacacttcat ggcggttggt
      121 tttatgcctg tctaagtttt tgtgtcgtgc atacctgaag atttcagacg gcatcggttt
      181 atgctgtctg aaaagtgtat tccgtttcag tttgtaagct atggcagtct gtttgtcttg
      241 tgttttgcgc aattgccctt attttgagcc gtgattttat tttgaattag atgaaaaaat
      301 gagtaatcaa gatttttatg cgacgctggg tgtggcaaga acagctaccg atgatgagat
      361 taaaaaagcc taccggaaat tggcgatgaa ataccatccc gaccgcaatc ctgacaataa
      421 agaggcggaa gagaagttta aagaagtaca aaaggcgtat gaaactttgt ccgacaagga
      481 aaagcgcgct atgtacgacc agtatggtca tgcggcgttt gaaggcggcg gacagggggg
      541 cttcggaggg tttggcggat ttggcggtgc gcagggtttt gactttgggg atattttcag
      601 ccaaatgttt ggaggcggtt cggggcgcgc ccagcctgat tatcagggtg aggacgttca
      661 agtcggtatc gaaatcacgc ttgaagaagc cgcaaaaggt gtgaagaaac gcatcaatat
      721 tccgacttat gaagcgtgtg atgtctgtaa cggcagtggc gcgaaaccgg ggacatcccc
      781 ggaaacctgc ccgacttgca aaggttcggg tacggtgcac atccagcagg cgattttccg
      841 tatgcagcag acttgtccga cctgccacgg tgcgggcaaa cacattaaag aaccttgcgt
      901 caaatgccgt ggcgcggggc ggaataaggc ggtcaagacg gtggaagtca atattcccgc
      961 cggtatcgat gacgggcagc gtatccgttt gagcggcgaa ggcggaccgg gtatgcacgg
     1021 tgcgcctgcc ggcgacctgt atgtaaccgt tcgcattcgg gcgcataaga ttttccaacg
     1081 cgacggtctg gacttgcatt gcgaactgcc gatcagtttt gccacggctg ctttgggcgg
     1141 ggagttggaa gtgccgacct tggacggaaa ggtcaagctc accgtcccca aagaaaccca
     1201 aaccggcagg aggatgcgcg tgaagggtaa gggtgtcaaa tctttacgca gcagcgcgac
     1261 cggcgatttg tactgccata ttgttgtcga aacgcctgtc aatttgaccg accgtcaaaa
     1321 agagcttttg gaagaatttg agcggatttc taccggcttg gaaaaccaaa caccgcgcaa
     1381 gaaatcgttt ttagacaagc tgcgcgattt gtttgattga ttttaaggtt cggaaacaag
     1441 cagccgtatc ggggaatctc cctgatatgg ctgtttttgt ttgtttaaaa atagttttta
     1501 ttttcaatgg ggtatggggc agggtgggat aactgttttt aactgttctt tttaaaactt
     1561 gacatcatgg cgcgatgcca acaatatgcg ggaatctgtt gtcaaaggaa gaataatgaa
     1621 taaatcttta tccggttcgg tagaagaata ccgcgaactg acgctccgag gcatgatact
     1681 cggtgcattg attactgtaa tttttactgc gtccaacgtt tacctcggtt tgaaagtcgg
     1741 actgaccttt gcctcgtcga ttccggcggc ggtaatttcg atggcggttt taaagttttt
     1801 caaaggcagc aatattttgg aaaacaacat ggtgcagacc caagcttcgg cggcgggtac
     1861 gctttcgacc atcatcttcg tcctgcccgg tttgctgatg gcgggctact ggagcggttt
     1921 cccgttctgg cagacgacgc ttttatgtat tgccggcggg attttggggg tgattttcac
     1981 cattccgctg cgttacgcga tggtggtgaa aagcgatttg ccttatccgg aaggtgtggc
     2041 ggctgctgaa attttgaaag tgggcggtca tgaagaaggg gataaccgtc agggcggcag
     2101 cggcatcaaa gagctggcgg ccggcggtgc gttggcggga ttgatgagct tttgcgccgg
     2161 aggtctgcgt gtgattgccg atagcgcgag ttattggttt aaaagcggta cggcaatttt
     2221 ccaactgccg atgggctttt cactggcatt gttgggcgcg ggctatttgg tcggactgac
     2281 gggcggtatc gccattctgt tgggcatttc gattgcttgg ggcattgccg tgccgtattt
     2341 ttcatcgcac atcccgcagc cttccgatat agaaatggcg gcgtttgcga tgaagctgtg
     2401 gaaggaaaaa gtacgcttca tcggcgcggg aaccataggt atcgcggcgg tttggacgct
     2461 tttgatgctg ctcaagccaa tggtggaagg catgaagatg tcgttcaaga gttttggcgg
     2521 cggtgcgccc gcaacagaac gcgctgaaca ggatttgtcg cctaaagcca tgattttctg
     2581 ggtattgtct atgatgttta ttctaggcgt gtcgttttac cactttatcg gcgattcgca
     2641 cattacgggc ggcatggctt ggcttttggt ggtcgtttgc acgcttttgg cttccgtcat
     2701 cggctttttg gtcgccgccg cctgcggtta tatggcgggt ttggtcggct cgtcttccag
     2761 cccgatttcc ggcgtgggca tcgtttccat cgtcgtgatt tcgctggttt tgctgctggt
     2821 gggcgaatcc ggcggtttga tggcggatga ggcaaaccgc aaatttttgc tggcactgac
     2881 tttattttgc ggctcggcag taatctgcgt ggcttcgatc tccaatgaca acctgcaaga
     2941 cctgaaaacc ggctacctgc tcaaagccac gccttggcgg cagcaagtcg ccctgattat
     3001 cggctgtatc gtcggtgcgc tggttatttc acccgtgttg gaactgcttt acgaagccta
     3061 cggctttacc ggcgcaatgc cgcgcgaagg tatggacgcg gcgcaggctt tggcagcccc
     3121 tcaagcgact ttgatgacga ccatcgcgtc gggcattttc gcccacaacc ttgaatgggt
     3181 ctatatcttt accggcatcg cgattggcgc ggtgttgatt gccgccgatt tggtgttgaa
     3241 aaaatcatca ggcggcaaac ttgccctgcc cgtcctcgcg gtcggtatgg gcatttatct
     3301 gccgccgtcc gtcaatatgc ccatcgtggc aggcgcggtg ttggcggcgg tgttgaaaca
     3361 catcatcggc aaaaaagcgg aaaaccgtga aggccgtctg aaaaacgccg accgtatcgg
     3421 tacgcttttc gcggccggtc tgattgtcgg tgaaagtctg attggtgtga ttatggcgtt
     3481 tattattgcc ttctccgtga ccaacggcgg ctcggacgcg ccgctcgcgt tgaatctgca
     3541 aaactgggat gccgccgctt cttggctggg cttggcgttc ttcgttaccg gaatgattat
     3601 ttttgcacgg cgcgtactga aggcgggtag gtagcctgtc ggagaaaatg ccgtctgaaa
     3661 cgttcagacg gcatttttta tcgggaaagt gtaaggcgga gcttttcggc ttgcgcccac
     3721 gttttgccgg caag
//
LOCUS       DAXHSJ010000169         3700 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000169 DAXHSJ010000000
VERSION     DAXHSJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.169"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            330..671
                     /locus_tag="ACFM8U_001841"
     CDS             330..671
                     /locus_tag="ACFM8U_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6335024.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGTSAPDFNAGGT
                     GIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPRICIPE
                     TLQTQMTHTRI"
     gene            668..3529
                     /locus_tag="ACFM8U_001842"
     CDS             668..3529
                     /locus_tag="ACFM8U_001842"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFC6335025.1"
                     /translation="MINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNYTAYMRKEAPEDGGGKDIKASFDDEAVIETEAKPTDIRHVKEIGHIDLVSHI
                     IGGRSVDGRPAGGIAPDATLHIMNTNDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFG
                     TTSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNML
                     FIFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLS
                     APYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIG
                     AVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKG
                     GSQLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIV
                     YLADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAG
                     YLNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRG
                     NAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMP
                     GIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLD
                     HNATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTW
                     SENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGT
                     LMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGL
                     AGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVA
                     GLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1002 a         1007 c          983 g          708 t
ORIGIN      
        1 taaagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttcatcca
       61 ctataactag ggaaattcaa attaagttag aattatccct atgagaaaaa gccgtctaag
      121 ccggtataaa caaaataaac tcattgagct atttgtcgaa agttcaaatt tccattttaa
      181 aacaattagt aaaatcgagt ttatcctagt tgtccaagac aacccctata ataatataat
      241 tcaaaatata aaaatgggtt acatctaaac attacggaat ttttattccc tcgcctgaat
      301 tctattgtca gattcaagga gacctcatca tgcgaacgac cccaaccttc cctacaaaaa
      361 ctttcaaacc ggctgccatg gcgttagctg ttgcaacaac actttctgcc tgcttaggcg
      421 gcggcggcac ttctgcgccc gacttcaatg caggcggcac cggtatcggc agcaacagca
      481 gagcaacaac agcgaaatca gcagcagtat cttacgccgg tatcaagaac gaaatgtgca
      541 aagacagaag catgctctgt gccggtcggg atgacgttgc ggttacagac agggatgcca
      601 aaatcaatgc ccccccccga atctgcatac cggagacttt acaaacccaa atgacgcata
      661 caagaatttg atcaacctca aacctgcaat tgaagcaggc tatacaggac gcggggtaga
      721 ggtaggtatc gtcgatacag gcgaatccgt cggcagcata tcctttcccg aactgtatgg
      781 cagaaaagaa cacggctata acgaaaatta caaaaactat acggcgtata tgcggaagga
      841 agcgcctgaa gacggaggcg gtaaagacat taaagcttct ttcgacgatg aggccgttat
      901 agagactgaa gcaaagccga cggatatccg ccacgtaaaa gaaatcggac acatcgattt
      961 ggtctcccat attattggcg ggcgttccgt ggacggcaga cctgcaggcg gtattgcgcc
     1021 cgatgcgacg ctacacataa tgaatacgaa tgatggaacc aagaacgaaa taatgtctgc
     1081 agccatccgc aatgcatggg tcaagctggg cgaacgtggc gtgcgcatcg tcaataacag
     1141 ttttggaaca acatcgaggg caggcactgc cgaccatttc caaatagcca attcggagga
     1201 gcagtaccgc caagcgttgc tcgcctattc cggcggtgat aaaacagacg agggtatccg
     1261 cctgatgcaa cagagcgatt acggcaactt gtcctaccac atccgtaata aaaacatgct
     1321 tttcattttt tcggcaagca atgacgcaca agctcagccc aacacactga ccctattgcc
     1381 attttatgaa aaagatgctc aaaaaggcat tatcacagtc gcaggcgtag accgcagtgg
     1441 agaaaagttc aatggctcca accattgcgg aattactgcc atgtggtgcc tatcggcacc
     1501 ctatgaagca agcgtccgtt tcacccgtac aaacccgatt caaattgccg gaacatcctt
     1561 ttccgcaccc atcgtaaccg gcacggcggc tctgctgctg cagaaatacc cgtggatgag
     1621 caacgacaac ctgcgtacca cgctgctgac aacggctcag gacatcggtg cagtcggcgt
     1681 ggacagcaag ttcggctggg gactgctgga tgcgggtaag gccatgaacg gacccgcgtc
     1741 ctttccgttc ggcgacttta ccgccgatac gaaaggtaca tccgatattg cctactcctt
     1801 ccgtaacgac atttcaggca cgggcggcct gatcaaaaaa ggcggcagcc aactgcaact
     1861 gcacggcaac aacacctata cgggcaaaac cattatcgaa ggcggttcgc tggtgttgta
     1921 cggcaacaac aaatcggata tgcgcgtcga aaccaaaggt gcgctgattt ataacggggc
     1981 ggcatccggc ggtagcctga acagcgacgg cattgtctat ctggcagata ccgaccgatc
     2041 cggcgcaaac gaaaccgtgc acatcaaagg cgatctgcag ctgggcggcg aaggtacgct
     2101 gtacacacgt ttgggcaaac tgctgaaagt ggacggtacg gcgatgaccg gcggcaagct
     2161 gtacatgtcg gcacgcggca aaggggcagg ctatctcaac cgtaccggac aacgtgttcc
     2221 cttcctgagt gccgccaaaa tcgggcggga ttattctttc ttcacaaaca tcgaaaccga
     2281 cggtggtctg ctggcttccc tcgacagcgt cgaaaaaaca gcgggcagtg aaggcgacac
     2341 gctgtcctat tatgtccgtc gcggcaatgc ggcacggact gcttcggcag cggcacattc
     2401 cgcgcccgcc ggtctgaaac acgccgtaga acagggcggc agcaatctgg aaaacctgat
     2461 ggtcgaactg gatgcctccg aatcatccgc aacacccgag acggttgaaa ctgcggccgc
     2521 cgaccgcaca gatatgccgg gcatccgccc ctacggcgca actttccgcg cagcggcagc
     2581 cgtacagcat gcgaatgccg ccgacggtgt acgcatcttc aacagtctcg ccgctaccgt
     2641 ctatgccgac agtaccgccg cccatgccga tatgcaggga cgccggctga aagccgtatc
     2701 ggacgggttg gaccacaacg ctacgggtct gcgcgtcatc gcgcaaaccc aacaggacgg
     2761 tggaacgtgg gaacagggcg gtgttgaagg caaaatgcgc ggcagtaccc aaaccgtcgg
     2821 cattgccgcg aaaaccggcg aaaatacgac agcagccgcc acactgggca tgggacacag
     2881 cacatggagc gaaaacagtg caaatgcaaa aaccgacagc attagtctgt ttgcaggcat
     2941 acggcacgat gcgggcgata tcggctatct caaaggcctg ttctcctacg gacgctacaa
     3001 aaacagcatc agccgcagca ccggtgcgga cgaacatgcg gaaggcagcg tcaacggcac
     3061 gctgatgcag ctgggcgcac tgggcggtgt caacgttccg tttgccgcaa cgggagattt
     3121 gacggtcgaa ggcggtctgc gctacgacct gctcaaacag gatgcattcg ccgaaaaagg
     3181 cagtgctttg ggctggagcg gcaacagcct cactgaaggc acactggtcg gactcgcggg
     3241 tctgaagctg tcgcaaccct tgagcgataa agccgtcctg tttgcaacgg cgggcgtgga
     3301 acgcgacctg aacggacgcg actacacggt aacgggcggc tttaccggcg cgactgcagc
     3361 aaccggcaag acgggggcac gcaatatgcc gcacacccgc ctggttgccg gtctgggcgc
     3421 ggatgtcgaa ttcggcaacg gctggaacgg cttggcacgt tacagctacg ccggttccaa
     3481 acagtacggc aaccacagcg gacgagtcgg cgtaggctac cggttctgac ggacaggaag
     3541 cagacagccg caaagatcac ggtctttgcg gctgtttctt atgaaaagaa aaccctattc
     3601 caattgcctg cttctattgt ttcaagactt cttccaaaga ttcggcatca atcagatgta
     3661 tagcggatta acaaaaatca ggacaaggcg gcgaagccgc
//
LOCUS       DAXHSJ010000170         3615 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000170 DAXHSJ010000000
VERSION     DAXHSJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.170"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(214..1278)
                     /locus_tag="ACFM8U_001843"
     CDS             complement(214..1278)
                     /locus_tag="ACFM8U_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6335026.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLYALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="ACFM8U_001844"
     CDS             complement(1279..2037)
                     /locus_tag="ACFM8U_001844"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6335027.1"
                     /translation="MKKVSVLIVARNEANHIRECIESCRFDKEVIVIDDYSTDNTAEI
                     AESLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="ACFM8U_001845"
     tRNA            complement(2112..2187)
                     /locus_tag="ACFM8U_001845"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..3350)
                     /locus_tag="ACFM8U_001846"
     CDS             complement(2241..3350)
                     /locus_tag="ACFM8U_001846"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFC6335028.1"
                     /translation="MEAQLGAPIENYAPPKHWTRKQLRSIERVSYLCVDAAEQALTDA
                     GLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGMSRNFSANTYVRMMPHTAAAN
                     IGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFFPSEVYVFDS
                     LYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAIIYAELVGYGA
                     NSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHHNDSMESRAVAA
                     VFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQSNPEGKLPPQLWDGQNDPDLP
                     AINLTGSGSRWETEKRIAASSSFAFGGSNCVLLIG"
     gene            complement(3354..3615)
                     /locus_tag="ACFM8U_001847"
                     /pseudo
     CDS             complement(3354..3615)
                     /locus_tag="ACFM8U_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          817 a          882 c          955 g          961 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgat gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta tttttaagga
      121 tggattcggg gtgtttcttt ccaatgaacg aatataattt ggcggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc agtttatatt gcatccaata atttgtcggc
      241 ggtatggttg atggtaaaac ggttggcgac gcattgatgt cctgccgttg ccagtgcgtt
      301 tttcgtttca gggttcaaga cgaggcgttc gatttcgtcc gccaaagatt caggtgtcag
      361 cctgtccagc agaatccccg attgatgatg ttcgataatt tccttttgcg cccccaaagt
      421 attggaaatc accgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggcgg taaaacgaag caagtttttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatccggat gtccgtcccc tgccaatttg agcctgattt gaggatattt
      661 ccgatgcagt atcacacagg cttcaatcag gttttccaat cctttttctg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaacg
      781 gccggtatcg ataccgttat gaacaatccg gtatttttct ttaaagggat tgtcggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tcggtgtttt tctgtatcag
      901 gcggtgataa aaatcggttt tgttggcgac gacattatgt ttgacaaata tcaggcgcac
      961 tccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggtttttcc ggataaagcg ggtaagtgcg tagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgtgtcg tgaaaacgga agaaacttcg gaaaagcgtc tgtgcatcaa
     1141 ctcattattt ttattgacgg cggtaaacat cgtgcagccc cgcagtccca atgcttttga
     1201 aacatcatag acatattgtt cgcccccgcc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccggatttg tacagataat atagtttgac
     1321 atatttaatc atcgtgtaat agctgtggtt gacggacata atccaaccca ttttcccatc
     1381 gagaaaccct ttgttcagga tataaatttt gaaaaaaccc caaatcggac ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg agatggaagt
     1501 gtatttgttg aatttgttga aatattgttc ccagttgtcg tatgtgtaat ggtacataaa
     1561 atgcttcaga cggcgtttgg ggtagggggt ttgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgtct tttttcggca tcaaacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ttcgacaaaa taggcggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattccccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccgttcaaa tgccgtctga agacttttgc gcccaaactc tcggcaattt cggcagtatt
     1921 gtcggtgctg tagtcgtcga taacgatgac ttctttatcg aaacggcaac tttcaataca
     1981 ttcccgaatg tggtttgctt catttctagc aacaatcaat acgctgactt ttttcatcat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtgggattga caaacttatt tcatccgatg agtaaaacgc agttgcttcc cccaaaggca
     2281 aacgacgagc tggcggcaat gcgtttttcg gtttcccagc ggctgccgct gccggtcagg
     2341 ttgatggcgg gcaggtcggg gtcgttctgc ccgtcccaaa gctggggcgg aagtttccct
     2401 tcgggattgc tttgccggtc ggcaatgccc cacgcgaacg cggcttcgat tgcgcccgcc
     2461 gcgcccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcgcggct ttccatactg tcgttgtggt gcgtcccggt gccgtgcagg
     2581 ttaatccagc cgatgtcttc gggcgcaaga cctgcgtgtt gcaatgccgt ctgaaaggcg
     2641 aggattgcgc cttgagcgtc ggggcgcggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattatc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacactt cggacgggaa aaattcttcg cctccgcccg ccagcatcat atcggtcaga
     2941 ccgtatttga tggcttcgta ggcataacct atgccttggc tgccggacga acacgcgctc
     3001 gatgtcggga tgatgcgtcc tttcagcccg aaaaagatgc cgatattggc ggcggcggtg
     3061 tgcggcatca tacgcacata ggtgttggca ctgaagttgc gcgacatgcc ggtcagcaac
     3121 aattcgccta catcgccgat gtctttggtg ctgccgctgg aagagccgca ggcaacgccc
     3181 atccgtccgt cggtaatgct ttcgtccccg agcaaaccgg catccgtcaa agcctgctcc
     3241 gccgcatcga cgcacaaata cgacacgcgc tctatacttc tgagctgttt gcgcgtccaa
     3301 tgtttcggcg gcgcgtaatt ttcaatcggc gcacccagtt gcgcttccaa ttcggggaaa
     3361 cgttcgcgcc aatccactat attttcgcaa gacgcgtttt gcctgattcc aaaaattctc
     3421 aatgccgtta atgtggttct gacggtctgc aaactccttg gaatggttga tgcggtaatg
     3481 gataaaaccg ctcacgtcca acttgtcgca gctgctcaga ctatcggtat aaacaatact
     3541 gtccggcatg attttctttt tgatgacagg gagtaacgtt tcagacttgg cattatccac
     3601 cacaacggta tagac
//
LOCUS       DAXHSJ010000171         3604 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000171 DAXHSJ010000000
VERSION     DAXHSJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.171"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(115..1689)
                     /locus_tag="ACFM8U_001848"
     CDS             complement(115..1689)
                     /locus_tag="ACFM8U_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC6335029.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            2014..3114
                     /locus_tag="ACFM8U_001849"
     CDS             2014..3114
                     /locus_tag="ACFM8U_001849"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC6335030.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGTDELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVPHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WMAACWVNRIPGSPHKATGASKPCILGAGYYY"
     gene            3234..>3604
                     /locus_tag="ACFM8U_001850"
     CDS             3234..>3604
                     /locus_tag="ACFM8U_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6335031.1"
                     /translation="MKILLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPDSSTQYSYPA
                     YDTTATTKSDALSSVTTSTSV"
BASE COUNT          903 a         1087 c          885 g          729 t
ORIGIN      
        1 ctataccaaa cggcatatcc cgacagaaca gattgtgcat aaggcacaag cccgcacatt
       61 ccatcaacaa aatgccgtct gaacacgggt tcagacggca tcagtatttt acaatcagaa
      121 tactgcctgt aaaaccaagt agcagacaac cgacaatacg gcggcggcag gcagggtaat
      181 gacccacgcc aaaccgatgg gcttcatcag tttccagttg gcattgcggt tgaccagacc
      241 gatgccgagt accgcgccga ccaagatatg cgtactggac acgggcagcc ccatcagcga
      301 cgcgcccatc acgacggatg cggcggacag ttcggcggta aaacccgagg cggggtgcat
      361 ttcggccaaa ctcgtaccga cggttttaat cacctcttta ccgacaaacc acaaaccgac
      421 aatcagcgcg atgccgaaag tcagcatcgc aatcgggggg acgacatttt gcgcggcaac
      481 gctgttggta cgcaaaacat ccataatcgc ggcaaacgga ccgatggcgt tggcgatatc
      541 gttcgcaccg tggctgaatg cgaagccgca ggcggtaaag acctgcatcc atgaaaacat
      601 ctgaaaggtc gatttgccca agtctttacg cttgaggctt ttggcaaaaa caaacgtccc
      661 catccacacc gccgcgccta tcataaagat ggtcaggaag ctgttgacgt tgctcatccc
      721 caaatgcagg tttttcaagc ccttgaaaat cagcatagcg gaaatcatca tcgcgccgaa
      781 cgaagcgata aagggaatcc aagaatgcag cgctttgtag gaatcgacat tgtttttacg
      841 gttgtcgaac gcataaagac cgcggtaata ctccgattgc agctcttgcg gatcgaattc
      901 gggttcgtcg taaatttgcg cgtcgtgcgc cattttggtg gcgtactcga ctttttcggc
      961 ttcggacaaa ccctcgaaaa acaggcggtg ccgttcttta taggcctttt tttcctgctt
     1021 gatgcccttg agcgtgcctt ccgcccaagc gttgtaatct aagacgtttt tcttgacgcg
     1081 cgaaaacaga aaataggaca ccgcgccgcc caacacgggc gacaataccc aagaaacacc
     1141 aataccgccc agcttgcccc aacgtatcaa atcgcccgat gcggcatcgt tcattaccgc
     1201 catacatacc gcgctgccga caatgccgcc gataatggaa tgggtggtag ataccggaag
     1261 cccttttttc gaggcaaaca acagccacaa cgccgccgcc aaaagcgcgg acatcataat
     1321 aaacacaaac tgtatgggtt cgaaatcaac acccttcaaa tcgacgatgc ctttgcgtat
     1381 ggtattggtt acctcaccgc ccgcgatgac cgcgccgctg acctcaaata ccgccgcaat
     1441 cagcaaagcc tgcgggatgg tcagcgtacc cgcaccgacg ctggtaccga aagaattggc
     1501 aacatcgttg ccgccgatgt tgaacgccat aaacacgccg aacatcgtgg caataatgaa
     1561 taaggcggcg taattgtaat gggtataacc caagccccaa taaataaaat agccgactgt
     1621 acctaccagc atggcggcaa agacggcgtt gatggtttta acatttgcgc tcatttggat
     1681 ttgagccatg gcaatcttcc tttaatgtgg tggaaatcat gagtgttgcc gacactgtac
     1741 tgctgcctcc ggtcgaactg ccgtttcggg atatggcaga cgcatccttc cttctgaacc
     1801 gcatccgaat acccccaaac ctgaccgaat gcaaaactgc tgccggaaac ggtttgtatg
     1861 acacttctac tactggatgt tgcgggcgca ttataatatt tttccatccg tcttgaaaca
     1921 tttatttaca ctttatttac actgcggcgg caaatcggta tacgagcgtc aatacacgtt
     1981 aaaatggcgt tttgcaccag tttgggagtg atgatggaaa cacagcttta catcggcatc
     2041 atgtcgggaa ccagtatgga tggggcggat gccgtgctgg tacggatgga cggcggcaaa
     2101 tggctgggcg cggaagggca cgcctttacc ccctatcccg acaggctgcg ccgccaattg
     2161 ctggatttgc aggacacagg cacagacgaa ctgcaccgca gcaggatttt gtcgcaagaa
     2221 ctcagccgcc tatatgcgca aaccgccgcc gaactgctgt gcagtcaaaa cctcgcgccg
     2281 tccgacatta ccgccctcgg ctgccacggg caaaccgtcc gacacgcgcc ggaacacggt
     2341 tacagcatac agcttgccga tttgccgctg ctggcagaac ggacgcggat ttttaccgtc
     2401 ggcgacttcc gcagccgcga ccttgcggcc ggcggacaag gtgcgccgct cgtccccgcc
     2461 tttcacaaag ccctgttccg cgacgacagg gaaacacgcg cggtactgaa catcggcggg
     2521 attgccaaca tcagcgtact cccccccgac gcacccgcct tcggcttcga cacaggaccg
     2581 ggcaatatgc tgatggacgc gtggatgcag gcacactggc agcttcctta cgacaaaaac
     2641 ggtgcaaagg cggcacaagg caacatattg ccgcaactgc tcgacaggct gctcgcccac
     2701 ccgtatttcg cacaacccca ccctaaaagc acggggcgcg aactgtttgc cctaaattgg
     2761 ctcgaaacct accttgacgg cggcgaaaac cgatacgacg tattgcggac gctttcccga
     2821 ttcaccgcgc aaaccgtttt cgacgccgtc ccacacgcag cggcagatgc ccgtcaaatg
     2881 tacatttgcg gcggcggcat ccgcaatcct gttttaatgg cggatttggc agaatgtttc
     2941 ggcacacgcg tttccctgca cagcaccgcc gaactgaacc tcgatccgca atgggtagaa
     3001 gccgccgcgt tcgcatggat ggcggcgtgt tgggtcaacc gcattcccgg tagtccgcac
     3061 aaagcaaccg gcgcatccaa accgtgtatt ctgggcgcgg gatattatta ttgagcacaa
     3121 atgccgtctg aaagaccgtt ccgccgttca gacggcattt gtgcctcttt catttacaaa
     3181 cccttaaaat ccctttatac tcaaaactcg ttcaaaaaaa aacaaaccct gctatgaaaa
     3241 tcctgctcct cctcatccct ctcgtcctca ccgcctgcgg cacactgacc ggcattcccg
     3301 cccacggcgg cggcaaacgc tttgccgtcg aacaggaact cgtcgccgca tcgtcccgcg
     3361 ccgccgtcaa agaaatggat ttgtccgccc taaaaggacg caaagccgcc ctttacgtct
     3421 ccgttatggg cgaccaaggt tcgggcaaca taagcggcgg acgctactct atcgacgcac
     3481 tgatacgcgg cggctaccac aacaaccccg acagcagcac ccaatacagc taccccgcct
     3541 acgacactac cgccaccacc aaatccgacg cgctctctag cgtaaccact tccacatcgg
     3601 tctt
//
LOCUS       DAXHSJ010000172         3563 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000172 DAXHSJ010000000
VERSION     DAXHSJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.172"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            70..351
                     /locus_tag="ACFM8U_001851"
     CDS             70..351
                     /locus_tag="ACFM8U_001851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335032.1"
                     /translation="MIKGRLKNSFDKDAVAKEIDLQREVTQAFGKTPPKPQRPLPTNS
                     AIPEVTDGIRQPEPCRRPNCKTNPTQKTVRPTQKTARKIRTAVQTGCPV"
     gene            complement(348..728)
                     /locus_tag="ACFM8U_001852"
     CDS             complement(348..728)
                     /locus_tag="ACFM8U_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC6335033.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDGGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1205..3286
                     /locus_tag="ACFM8U_001853"
     CDS             1205..3286
                     /locus_tag="ACFM8U_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC6335034.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIAKYVMQLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTLLFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3276..3470
                     /locus_tag="ACFM8U_001854"
     CDS             3276..3470
                     /locus_tag="ACFM8U_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC6335035.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
BASE COUNT          797 a         1041 c          914 g          811 t
ORIGIN      
        1 tcattggata acagccctga agaaatcgga gcaggtttaa agttggcatt gagccgctgc
       61 cgataatatt tgataaaagg tcgtctgaaa aacagctttg acaaagacgc ggttgccaaa
      121 gagatcgacc tacaaaggga agtaacgcag gcgttcggca aaacgccgcc caagccgcag
      181 cggccgttgc cgacgaactc ggcaataccc gaagttacgg acggtatcag gcagcccgaa
      241 ccctgccgga ggccgaactg caaaacaaat ccgacacaaa aaaccgtccg tccgacacaa
      301 aaaaccgccc gaaaaatccg gacggcggtt caaacaggct gccccgttta acgggcgcgg
      361 caggaagttt cgaccgaatt gccgtaggca tcggtaaagc cgaaaaaggc ttcgccgcct
      421 ttctggtgcc actcggttgc gtttccgaac aaaccgtgtt cggcggtata gcgttcgccg
      481 gatgcggcaa cgtcggaaga gaggacggca cgcctgccgt ccagccgcaa cgcgactttg
      541 ccgctgtcca aatggcggac gcgcacagac aaaccgttct cgcaggaaaa cgcccgaaaa
      601 tcgtccgtgc cggcttggtt ttgaacaggc ggcatatgcc cgcgtccgcc gccatcatac
      661 gcttccggca cggcacaggc cgccaaagac aaaaccggta cggtcagcgc gaaaaacctg
      721 atattcataa aagctcccca ataaaaataa gatatgaaac aaccgccctg attccaagct
      781 gcggcaacgc catactataa acggacgcgc aaacacacaa gcccgataac cggaatttac
      841 ccgcgatgaa tcaataatcc ggattgcgcg ccccttcttt acccgtcttc cggcaccgcc
      901 ttttgcctga cgatgccgtc caaacccgcc tgtccgcccg aaggaatgta aattttttcc
      961 aaattccaag taaaaaccgc tatcagtgtg ctaatttgcg ttaaaatcct attcggcgtt
     1021 taacgttttg tgcgcccgca tccctgcact gtttgatgcg ggcataaggc acaaatcccg
     1081 acaagcgcac tgtttcatac ttcgtcaatc attcagactc cggtttgtgc ccgtgccggc
     1141 agatggttcg gccgtttccc gccgttcagg catattccga cagtgtgaga taaggattta
     1201 ttcgatgaaa tcactcaaaa ccttcctcat ttggggcata gtggtactgg tcggcttagc
     1261 atcctttacc actctggccc tcagccgagg cgaacaggtc agcgcggtat ggatggttac
     1321 cgccgccata tccgtttatt gcatcgccta ccgtttttac agtctctaca tcgccaaata
     1381 tgtgatgcag cttgatccga accgcctgac tccggcagaa cgccacaacg acggcttgga
     1441 ctacgttccg acgcacaagg gcgtattgtt cggacaccac tttgccgcga ttgccggcgc
     1501 gggccctttg gtcggcccgg ttttggcggc gcaaatgggt tacctgcccg gtactttgtg
     1561 gattatcttc ggcgtggtgt ttgccggcgc ggtacaggat atgatggtct tgttcgtctc
     1621 tatgcgccgc gacggtaagt ctttgggcga tattgtgaaa caggaactcg gcactgtccc
     1681 cggcgtgatt gcctccatcg gtattttgat gattatggtc atcattatgg cggtgttggc
     1741 gttgattgtc gtaaaagcac tggttcacag cccttggggt acgttcacca ttgccgccac
     1801 tatgccgatt gccctgttta tgggtattta cacgcgctat atccgtccgg gcaaaatcgg
     1861 cgagatttcc atcgtcggct ttattttgct gatgctggcg gtaatttacg gcgaagatgt
     1921 ggcgaaaagt tccatcggac attggttcga ccttgacggc atccagctca cttgggcgat
     1981 tatgatttac ggctttgtcg cctccgtatt gcccgtatgg ttgctgctca ctccgcgcga
     2041 ctatctctcc accttcctga aaatcggtac gattgcggcc ttggctttgg gcatcgtcat
     2101 cgtcaatccc actttgcaaa tgcctgccgt aacccacttt atcgacggtt cgggtccggt
     2161 attctcaggc gcattgttcc cgttcctctt catcaccatc gcctgcggtg cggtttcagg
     2221 cttccacgcg ctgatttctt ccggcaccac gccgaaaatg ctggaaaacg aaacccacgt
     2281 ccgtatgatt ggttacggcg gtatgttgat ggaaagtttc gtagccatta tggcgcttgc
     2341 cgccgccgca tcgcttgatc ccggcgtgta cttcgccatg aacagccctg ccgccctgat
     2401 cggtacggat gccaacaccg ccgccgaagt gattaccacc aagctgcaat tccctgtcga
     2461 tgccgcaacg ctgttgcaca ccgctaaaga agttggagaa aacaccatcc tgtcccgtgc
     2521 cggcggtgcg cccaccctcg cagtcggtat ggcgcacatt atgagccgcc tgattccggg
     2581 cgaggcgatg atggcgttct ggtatcactt cgccctgttg tttgaagcct tgttcatcct
     2641 gaccgccgtc gatgccggta cgcgcgtcgc acgttttatg attcaagact tgggcagcat
     2701 cttctacaaa cctttcggca acaccgactc catcccagcc aacctgattg cgaccttctt
     2761 cgccgtggca ttgtggggct actttctcta caccggcgta accgacccgc tgggcggcat
     2821 caactcgctc tggcctttgt tcggcatcgc caaccaaatg ctggcgggcg tggccttgat
     2881 tatgtgtgcc gtggtgctga ttaagatgaa acgcgaccgt tatgtttggg tggtactcgt
     2941 tcccgccgtc ggcgtactgt tcgtaacctg ctacgccggc ctgcaaaaac tgttccacag
     3001 cgatccgcgc atcagcttcc ttgcccacgc cggcaaatac agcgacgcat tggctaaaaa
     3061 cgaagtcctt gcgcctgcca aagacatcgg cgaaatggcg caaatcatct tcaacgacaa
     3121 gattaatgcc ggtctgaccc tcctcttctt gtcggttgtc gtgattgtcg ccgcgtacgg
     3181 tttgcgtacc gccctcaaag cacgcaaagt cggctggccg accgccaaag aaatcccggc
     3241 ggtgtaccgc gacggcaaac agccggaggc acaaagtgaa gcataagctc gcgtcttggt
     3301 ggaaaaccat caagctgacg gcaaacttga tggcaggcgt gcccgattat gaaaactacg
     3361 ttgcacagca gcgcaaacat aatcccaacg cccccgtgat gaccaagctg cagtttcaag
     3421 actattgccg caaacgccgc tgcggcgcaa acggcggacg ctgctgttag ccccgcctga
     3481 aacaaatgcc gtctgaacgc cgcttcagac ggaattttta taatatagtg gattaacaaa
     3541 aatcaggaca aggcgacgaa gcc
//
LOCUS       DAXHSJ010000173         3550 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000173 DAXHSJ010000000
VERSION     DAXHSJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.173"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(1022..1792)
                     /gene="xth"
                     /locus_tag="ACFM8U_001855"
     CDS             complement(1022..1792)
                     /gene="xth"
                     /locus_tag="ACFM8U_001855"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6335036.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1846..2133)
                     /locus_tag="ACFM8U_001856"
     CDS             complement(1846..2133)
                     /locus_tag="ACFM8U_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFC6335037.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            2235..2468
                     /locus_tag="ACFM8U_001857"
     CDS             2235..2468
                     /locus_tag="ACFM8U_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335038.1"
                     /translation="MDDCTGLYVPFPTFFANTRKGGLSDGIKLPPLPCLTLLPNGIIR
                     IVQKMPYNRRTSCPADASVPSGKQNTCLKRCFF"
     gene            complement(2601..3482)
                     /gene="nadC"
                     /locus_tag="ACFM8U_001858"
     CDS             complement(2601..3482)
                     /gene="nadC"
                     /locus_tag="ACFM8U_001858"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC6335039.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAKYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAILVKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
BASE COUNT          864 a          857 c          893 g          936 t
ORIGIN      
        1 tgaaactgat gcagatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
       61 cagtacaaat agtacggcaa cgctgtactg gtttaaattt aatccactat attttgtaat
      121 agctgccatt atgcctacta ggatatatac cttctatata atccttattt aaataaaaac
      181 ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa gattttatct ttctcatcag
      241 gtttatattt gctatcaata tcaaaaagag aataaggatt acaatgagtt cgacatacta
      301 ggtaaaaatt tatatcttgg atcatgaaat attcgatatt tatcaggatc gaaagatgct
      361 aaaaattcat caatatcggt actggatatt ttgtcatcac taataaacat cgctagagat
      421 gggcgacttt gattttccat ttgttgatta gtaaaggata taaatactaa cttgccattg
      481 tcaaaccaca tagatttata tagtggatta aatttaaacc agtacgtcgt tgcctcgcct
      541 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
      601 tatagttatt gcctttggga gaatcaattc tgacagcaca taacataggt tcatctttac
      661 agtcgttata ttgggtttca tcaaactcaa ctgcatagca caataaaggc aataggaaaa
      721 aaaggagatt aatttattac tatacataag gcagcctgac tattcattct aaaataaatc
      781 cggtttttaa tatttgattc tgctccggat tatatacata ataaatttta ctgctccaaa
      841 atctgatatt aaccctatgg atttcataaa tttttcagaa aaataggctg tgcttgaaca
      901 atccgccgac tccggctgtt tgtcagggaa aaggcaatta ttttcggtta gtcagcagcc
      961 tatcctattg aatcacttaa agatatggat gtttgtcaac gccatatcaa aacacggtct
     1021 tttaccaatc gaattctgcc gtcaccggcg cgtggtcgct cggacgctcc agcgcgcgcg
     1081 tctccaaatc aacgcggaca tccttcaacg ccgccgccat cgcaggcgac accaaaatat
     1141 ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc gcgatagtcg aaccaggtat
     1201 agaacgcgcc ttcgggatgg acttggcgca ggctgtcggt cagtcccaaa tccagcaggt
     1261 ttttgaacca ctgccgttcg acggacgaac agtggatttt ttcgtgccat ttttcagggt
     1321 cgtaacagtc cgcatcggca ggcgcgatat tgaaatcgcc cagtagcacc agtttgccgt
     1381 agcgggtcat ttcatcgcgg acaaattcgg ttaacgcggc aaaccattgt tctttatatt
     1441 tgaatttcgg gctgtcgagg gcttcgccgt tgacgcaata gacattgatg acgcgcacac
     1501 cgccgacggt tgccgcaatc acgcgccgtt gcggatcgtc cggcagcgcg ggcagcccga
     1561 tatgcacgtc ttccggcgcg ttgcggctga cgattgccac gccgttgtag gttttctgcc
     1621 cgctccaaac acagtgccag cccatcattt gcaaagcggc ggccggaaat ttgtcctgat
     1681 cgagtttgag ttcctgcaaa accaaaatat cgggcggatt gtcggcaagc aggttttgca
     1741 cctgcggcag ccgcacattg agcgaattga cgttccaagt ggtgattttc ataacatttc
     1801 cgaaaaatgc cgtctgaaac gttcagacgg caaaaggaaa ggattttaac accataccgc
     1861 gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt gccgcgtctt cggaaatcag
     1921 gcggtattcg ataatgcggt ggtaacgcgt aaagtccacc agaccgcccg ccctcaggcg
     1981 gttcaaatgg ccggaaacgg cggtggccgg cagggataag gattttgcca gttcggcgat
     2041 attgcgttcg ctgtccaaca gttggatcag tatcgccata cgttcgggat gcgccataag
     2101 tttgagtatg gtgtggagcg gtattgtgtt catcggtatt cctaaaacct ttgccgcccg
     2161 ccaagcggat tgacgggcga cagatatgga tgattgtgat tatctttata aatcgtatta
     2221 taaatcaaat atttatggat gattgtacag gattgtatgt tccttttccc actttttttg
     2281 caaacacgcg taaaggcggg ctttcagacg gcataaaact gccgccccta ccctgcctca
     2341 ccctgttgcc gaacggaatc atccgcattg ttcaaaagat gccttacaac cgccgcacgt
     2401 cttgccctgc cgatgcctcc gtgccttcgg gcaaacaaaa tacctgcctg aaacgctgtt
     2461 ttttctaaag catcacgacc ttcacgaaaa atgccgtctg aagaatcttt cagacggcat
     2521 ttcctccttg aggcaataac cggattacgg ttaggcggtt ccttgtctga catatcagac
     2581 aactgccgac accctaaaac tcatgccaag aaatccaaac ctatgtccaa cgagcggctg
     2641 ctgtgggtca gatagccgag ggcgatgccg tccacgccgg tttgcgccac gcgcttcagg
     2701 cggtcgaagc cgatgccgcc cgatgcttcg caatagacgg tgtgggggtg ggcggtttgc
     2761 gtgtggcagc ggtttgccgc ttctttcagg gtttcgtcgt ccatgttgtc cagcaaaatc
     2821 cgttccgcgc ccgctgcgat ggcttcgtcc agttgtgcca acgtgtccac ttcgatttct
     2881 acacaagtca acggtccgac cgcctgtttt gcctgccgca cggcttgggc gatgctgccg
     2941 caataggcga ggtggttgtc tttgacaagg atggcatcgt ccaaacccat gcggtggttc
     3001 acgccgccgc ccgccctgac ggcgtatttt tgcaggacgc gcagcagggg gatggttttg
     3061 cggctgcaca cgatgtctgt accgtatttg gcgacttcgg caacggcacg cgcggtggcg
     3121 gtggcgatgc cgcttaagtg cgtgaggtag ttgagcgcgg tgcgttcggc ggcgagcagc
     3181 gcgcaggcgt tgccttcgac ggcggcaagc gtctgacctg cgcggacgac ttgcccgtct
     3241 cggatttcgg cttggaagcg gacggacgga tccatcgtct gaaaggcgag gcgcgccaag
     3301 tccatgccgg cgataacgcc gtcttcgcgg ctgacaagga agagtttggc ggttttgtcg
     3361 ggggcgatga cggcggcgga cgtaatatcg ccgcgcctgc ccaaatcttc gctcaaggct
     3421 tgttcgacta tggggcgcaa cagggtgtcg ggtagggaaa agagggtgtt ttcagacggc
     3481 atggtgtttc ctttgttgaa aaagattgat attgcccgat ggggcaaaac ttcattttat
     3541 agtggattaa
//
LOCUS       DAXHSJ010000174         3521 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000174 DAXHSJ010000000
VERSION     DAXHSJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.174"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(63..2240)
                     /gene="fetA"
                     /locus_tag="ACFM8U_001859"
                     /gene_synonym="frpB"
     CDS             complement(63..2240)
                     /gene="fetA"
                     /locus_tag="ACFM8U_001859"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC6335040.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDDDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIDR
                     QAPAYRETTQSNTNLEYTGKNLGFVEKLDANAYVLEKKRYSADDKGSGYAGNVEGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGDFTISSKKKDPNNPKNE
                     IDKDPTEIAKDKEDMALVHSYKLSNPTKTDAGVYVEAIHDIGDFTLTGGLRYDRFKVK
                     THDGKTVSSSSLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIAD
                     GTKAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIK
                     NHGYELGASYRTGGLTAKVGVSYSKPRFYGYDTHKDKLLSANPEFGAQVGRTWTASLA
                     YRFQNPNLEIGWRGRYVQKAVGSILVSGQKDRQGNLENVVRKGFGVNDVFANWKPLGK
                     DTLNVNLSVNNVFNKYYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          812 a          823 c          973 g          913 t
ORIGIN      
        1 aaattatcag aacagatgcc gtctgaaagg ctttcagacg gcattttttc gggatgtgcg
       61 ttttagaact tgtagttcac gcccaagcgt acatcacgtc ccacgcccgg cagggtattg
      121 gtccagcgtt ggctgtgcgg atagtagtac ttgttgaaca cgttgttaac cgaaagattg
      181 acattgagcg tgtctttgcc caatggtttc cagttggcga agacatcgtt cacaccgaaa
      241 cctttgcgta caacgttttc caagttgcct tggcggtctt tttgacctga caccaatatc
      301 gaacccacgg ctttttgaac atagcgtccg cgccagccga tttccagatt cggattttgg
      361 aagcggtagg caagggaggc tgtccaagtg cgaccgactt gtgcgccaaa ttcagggttc
      421 gcgctcaaca gcttgtcttt gtgcgtatcg taaccgtaaa agcgcggttt gctgtagctt
      481 acgccgactt tggcagtcag gccgccggtg cggtaggacg cgcccaattc gtaaccgtgg
      541 tttttgatgt aaccggcatt gacggcttca cggacggcga cagagtcgtg gcggttttgc
      601 ggattggcaa gcgcgtcttt gatggtttgc cagaagtagc tgccgtttgc ggcaaacgtg
      661 ccgtcgttgt agttgaagcc gatttcggta ttgcgcgcgc gttcggcttt ggtgccgtcg
      721 gcaatcgaga tgatgccgcg tttgccgtgg gtttgcagcg cgtcatacag gcgcgggctg
      781 cggctggcgt agttgtggct tgcgctgaag ctccagtgtt cgtgcggctg ccaaatcacg
      841 ccgaaactcg ggttaaggct gctgcttgaa acagttttgc cgtcgtgggt tttcacctta
      901 aagcggtcgt aacgcagccc gccggtcagc gtgaaatcgc cgatgtcgtg aatggcttca
      961 acatatacgc cggcatcggt tttggtcggg ttggacagtt tgtaggaatg gacaagagcc
     1021 atatcttcct tgtctttcgc tatttctgta gggtctttat ctatttcgtt tttaggattg
     1081 tttgggtctt ttttcttact ggaaatcgta aagtcgccgt tcaaaaacgc ttgcggtttg
     1141 atttcctgat ggcggtagtt gataccgtat ttcaacaggg tttgttcggc aaggcggctg
     1201 tcgaagttga aattcatacc ccgagtggtg attcgggtat ggttggggcc ttctacattg
     1261 cctgcgtaac cgctgccttt gtcatcggcg gaatagcgtt tcttttccaa tacataggcg
     1321 ttggcatcca gtttttcgac aaagcccagg tttttacccg tgtactccaa gttggtgttg
     1381 gattgggtgg tttcgcggta agcaggggct tggcggtcaa tatttatccg tgaacttttg
     1441 tcgccgacgg taaattcttc acgcacagtg cggatgcccc ggtgttggtc tttcatgtgg
     1501 ctcaacacga tgcggtggtc gtcgtcgccg aaagttgttc cgattttggc gaggtagctg
     1561 cgtttgtcca gcgcgctgta cggtacggtt ttgccgccgt tgacattgcg gaaacctttg
     1621 ccggcttcgt aatctttttc atcgttgcgg ttgtaagaga acaaaccgtc gaagttgccc
     1681 tcttttccga atacgctggc gccgtagctt acgccttcgt tgctggcaaa gccgctgttg
     1741 aggcgcacgc cccagttttt atccaagcct ttgagcaggt cttgggcatc gacggttttg
     1801 gcgataatcg cgccgttggt cgcgccgata ccggcagagg cggaacccgc gcctttttgt
     1861 acggaaacga ctttaaccaa agcgggatcg acaataaatc tgccttggtg gtaaaggatt
     1921 tggctgtcgg aataggcgtt gtccaccttg atgtcgacag agttctgacc cataccgcgc
     1981 agcgtcagga attgggacgt gccgttgccg ccgccgaaat cgatggacgg ctcttctttt
     2041 aagagttcgc gcatatcggt tgcggtgctt tcgtcttttt gttgaagcgt aacgatgttg
     2101 gtacggattt tgctgccttg gcggtcgcct tttacggtaa cggtatccaa tgcgacattg
     2161 gcattatttt ctgccgcatg ggcaaaacct gccgccaggg taagcgagag caggctgagg
     2221 cggaaaaacg gggcgttcat ttgttcgtcc ttttgagtgt atgaagggaa gtaaatccaa
     2281 accgttaagg tttggcagga taagaaaaat aataattatt tttgtttata ttaacgagag
     2341 gggggatttg tgtaaagctg attatcgttt ttatttgcga agtgttgttt tttgttgaca
     2401 ggttttgtcg gaaatgtata gtggactaac aaaaatcagg ataaggcgac gaagccgcag
     2461 acagtacaga tagtacggca aggcgaggca acgctgtact ggtttttgtt agtccactat
     2521 aaaaaacggc ggaaatatag tggattaaca aatgcgggaa tgacgaaaag caacctttcc
     2581 gcgtccttcc cgcgaaagcg ggaatctaga acccctaacg tggcaggaat ctatcggaaa
     2641 aaaccgaaac cgaacagact ggattcccgc ctgcgcggga atgacgaaaa gcaacctttc
     2701 cgcgtccttc ccgcgaaagc gggaatctag aacccctaac gcggcaggaa tctatcggaa
     2761 atgactgaaa ccgaacgaac ctagattccc gcctgcgcgg gaatgacgaa gcctgtgcgg
     2821 gaatgacgaa aagcaacctt tccgcgtcat tcccgcgaaa gcgggaatct agaaccccta
     2881 acgcggcagg aatctatcgg aaaaaaccga aaccgaacga acctagattc ccgcctgcgc
     2941 gggaatgacg aaaagcaacc tttccgcgtc cttcccgcaa aagcgggaat ctagaacctc
     3001 taacgtggca ggaatctatc ggaaatgact gaaaccgaac agactggatt cccgcctgtg
     3061 cgggaatgac gaagcctgcg tgggaatgac gaaaagcaac ctttccgtgt cattcccgca
     3121 aaagcgggaa tctaggaact caaagctgca agaatttatc agaaacaact ggaactcaaa
     3181 aaaactagat tcccgcctgc gcgggaatga cgaagggtta aagaaatgat gagaaacggc
     3241 ggtaatttat cgggaataac cgaaaacgaa cggaaatcag gacaaggcgg cgaagccgca
     3301 gacagtacaa ataggacgga accgattcac ttggtgcttg agcaccttag agaatcgttc
     3361 tctttgagct aaggcgaggc aacgacgtac tggtttttgt tcatccacta taacagcaac
     3421 cctgtcgccg tcattcccgc gaaagcggga atccagtccg ttcagtttcg gtcatttccg
     3481 ataaattcct gctgcttttc atttctagat tcccactttt g
//
LOCUS       DAXHSJ010000175         3510 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000175 DAXHSJ010000000
VERSION     DAXHSJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.175"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..218
                     /locus_tag="ACFM8U_001860"
     CDS             <1..218
                     /locus_tag="ACFM8U_001860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335041.1"
                     /translation="RYWKMKEMKPVRYHIGDMPETSKQTASRHDDRAVGVDDDCFHDS
                     FGQFLFGCSAEATRTAPISVKRSCLFT"
     gene            326..1360
                     /gene="lpxK"
                     /locus_tag="ACFM8U_001861"
     CDS             326..1360
                     /gene="lpxK"
                     /locus_tag="ACFM8U_001861"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC6335042.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            1567..2148
                     /locus_tag="ACFM8U_001862"
     CDS             1567..2148
                     /locus_tag="ACFM8U_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC6335043.1"
                     /translation="MKLKTLLLPFASLALCAGAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEEKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPAGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            2217..2399
                     /locus_tag="ACFM8U_001863"
     CDS             2217..2399
                     /locus_tag="ACFM8U_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC6335044.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARTLSEEELKA"
     gene            2396..3157
                     /gene="kdsB"
                     /locus_tag="ACFM8U_001864"
     CDS             2396..3157
                     /gene="kdsB"
                     /locus_tag="ACFM8U_001864"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC6335045.1"
                     /translation="MTEFVVLIPARLDSSRLPRKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTVCQAHGVEVVMTSNRHESGTTRLAEAAAALKLPQHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYLRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            3180..>3510
                     /locus_tag="ACFM8U_001865"
     CDS             3180..>3510
                     /locus_tag="ACFM8U_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC6335046.1"
                     /translation="MQQHIEKWQHLSREEQKILAEVWGLVQNDDQEVHYEMLKLNAPD
                     EASGEFWFRMAETLSTLPPNRSLDLRMNGGRLATAVSILSVMIEDNPDIPQLWAQKIT
                     ALNYSGLN"
BASE COUNT          863 a          873 c          982 g          792 t
ORIGIN      
        1 aaagatactg gaaaatgaaa gagatgaaac ctgtccgtta tcatattggc gatatgcccg
       61 agacttcaaa acaaaccgcc tcccgtcatg acgacagggc agtgggtgtt gacgatgatt
      121 gttttcatga ttcctttggt caatttttgt ttgggtgttc ggcagaggca acccgaaccg
      181 cgccaatttc tgtaaagcgc agttgcttat ttacctgatt ggttcgctta tcggtttggt
      241 cttcgcgttg tttataggtg ggtctgtatc gggtacgcat gattaatgcc ccgggctgat
      301 tttgcttcga ggatttgtat cgaatatgcc gaattgtttc aaatttcata ccgttattga
      361 acggcattgg caaaaacctt atccggtttt gtcttttctg cttaagccgc tctccgggct
      421 gtttgccaaa attgcggcaa aacggcggac ggatttttta tcgggaaaac ggcaaagcga
      481 aaagctgccc gtgcctgtgg tcgtggtcgg caatattcac gcgggtggga cggggaaaac
      541 gccgattgtt gccgcgctgg tgtcgggttt gcaggaaaag ggcgtcaagg tcggcatcat
      601 cagccggggc tacgggcgca agagcaaggc ggttcatgta ttgaatgctg agagccgagc
      661 ggaagatgcg ggcgatgagc ctttgctgct gttccgcaaa accggtgcgc cgacggcggt
      721 gggcagcagc cgtgcagagg caggcagggc gttgctggcg gcgcatcccg acatcggact
      781 gattgtggcg gacgacggtt tgcagcatta cgccctgcgg cgagatgtgg aaatcgcggt
      841 gtttccggcg gcggatacgg ggcgcacgga tttggattta ctgcccaacg gcagtttgcg
      901 cgaacctttg ttgcggctgg attcggtgga tgcggtcgtc gtcagcggcg gcaaggcgga
      961 tgcgctgttt aggccgtctg aaaatatgtt tcacagccgt atcgaagcgg gacggattta
     1021 ccgtttgaac aatccgtccg aaatactgga cacaggccgt ctgaaaaatc aaaccgtcgt
     1081 cgccgtggca ggtattgcca agccggcgcg ttttttcgat tcgttgcgga atatgggcat
     1141 taccgtgaag cgaaccgtcg cgctgcccga ccacgccgac atttcggcgg cagatttgcc
     1201 cgatgcggac gcggtcatta ttacggagaa agatgcggtc aaattttcag acggcatttg
     1261 caccgataat gtttgggtgt tgcccgtttg tgcgataatc gaacctgatt tggcggcgtt
     1321 tgtgttggag cggttggaag atgtaccgaa ggccgtctga aagcacggtt tgggcggagt
     1381 gattacggat ttgaataaga acgcctcgcg ccgtcattcc cgcgcaggcg ggaatctaag
     1441 tctcgaattt tcaggaatgc ctaggaggct tcagaaatcc caaatctccg gattcccgcc
     1501 tgcgcaggaa taatggaagc cggttgtatt ttttatctgc attaatcatt tgttaaagga
     1561 ttggatatga agctgaaaac cctgttatta cctttcgcct ctttggcatt gtgcgccggt
     1621 gcatttgccg ctccgcccag cgacgcgtcg ttggcgcgtt ggctggatac gcagaatttt
     1681 gaccgggata tagaaaaaaa tatgatcgag ggctttaatg ccggatttaa accgtatgcg
     1741 gacaaagccc ttgccgaaat gccggaagag aaaaaagatc aggcggcaaa agcctttaac
     1801 cgctatcgtg agaatgtttt gaaagatttg attacgcccg aagtgaaaca ggctgtccgc
     1861 aatactttat tgaagaatgc ccgtgagata tacacgcaag aagaaattga cggcatgatt
     1921 gccttttacg gttcgcccgc cggtcagtcc gtcgttgcca aaaatccgcg cttaatcaag
     1981 aaatcgatga gtgaaatagc ggtatcttgg actgcattgt cagggaaaat cgcgcgacat
     2041 catctgcccg cgtttacgga agagttgcgg cgcatcatct gcggcggtaa aaatcccgat
     2101 gcgggctgta aacaagccgg acaggttggg aaaaggcatc agaaataaat gatagccgtc
     2161 tgaaatattg aagagggcat ccgattgatt gaaccatcaa acccgaaagc aaccctatgg
     2221 aaaaaaaatt cttagacatc ctcgtctgcc ccgttaccaa aggcaggctg gaatatcatc
     2281 aggacaaaca ggaattgtgg agccgtcagg cgaagcttgc ctatccgatt aaagacggca
     2341 ttccctatat gctggaaaac gaagcgcgaa cgttgagcga agaggaactc aaagcatgac
     2401 cgaattcgtc gtattgattc cggcgcggct ggattcgtcg cgcctgccca gaaaagcctt
     2461 ggcggacatc cacggcaaac cgatggtcgt gcgcgttgcc gaacaggcgg caaaaagtaa
     2521 agctgcgcgc gtcgttgttg ccaccgacca tcccgatatt cagacggtct gtcaggcgca
     2581 cggcgtcgaa gtcgtcatga cttcaaaccg gcacgaaagc ggcacgacgc gccttgctga
     2641 agctgccgcc gcgctgaagc tgccgcaaca tctggtcgtt gtcaacgtac agggcgacga
     2701 gccgctgatt gcccccgaac tcatcgaccg caccgccgaa gtactcgtcg aaaacaacgt
     2761 ccaaatggcg accgccgccc acgaattgca cgatttcgac gaattgatga atcccaatgc
     2821 cgtcaaagtc gtcctcgaca aaaaccgcaa cgccatctac ttcagccgcg ccccgattcc
     2881 ctatctgcgc gatgcaatgc gtgccggaaa acgcgaaatg ccgtctgaaa ccgccgtcct
     2941 gcgccatatc ggcatctacg cttaccgcgc cggcttcctg caacgctatg ccgaaatgag
     3001 cgtttcgccg ctggaaacca tcgaatcgct ggaacagctg cgcgtcctgt ggcacggtta
     3061 tcccattgcc gtcgaaaccg ccaaagaagc ccccgccgcc ggtgtggata cgcaggaaga
     3121 tttggatagg gttcgcgctg tatttcagac cgtataaaac aggttcaaag ggaaaagata
     3181 tgcagcaaca tattgaaaag tggcaacact tgagccggga agaacagaaa atccttgccg
     3241 aagtatgggg tctcgtgcaa aacgatgatc aggaggttca ctatgaaatg cttaaattga
     3301 acgcacccga cgaagccagt ggcgaatttt ggttcagaat ggcagaaaca ctcagcaccc
     3361 tgccgcccaa ccgttccctc gaccttagaa tgaacggcgg caggctggcg accgccgtat
     3421 ccatcctttc cgtcatgatt gaagacaatc ccgacatacc gcagctttgg gcgcaaaaaa
     3481 ttaccgccct caattatagt ggattaaatt
//
LOCUS       DAXHSJ010000176         3483 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000176 DAXHSJ010000000
VERSION     DAXHSJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.176"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            36..713
                     /locus_tag="ACFM8U_001866"
     CDS             36..713
                     /locus_tag="ACFM8U_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC6335047.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTPSNKCDSGKKGQGMLAFGDKNGNK
                     GYDNDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYST
                     NQDLTNTSKFVYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRGDRRTR
                     RTMCQYK"
     gene            737..1351
                     /gene="pilV"
                     /locus_tag="ACFM8U_001867"
     CDS             737..1351
                     /gene="pilV"
                     /locus_tag="ACFM8U_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC6335048.1"
                     /translation="MKNNDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHHTLSVVDGDFQVGV
                     IKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGAAPSLSDSGTFSQNCDGSA
                     NGDTLIKVLWVNDSAGDSDIAHTNLETNGNNIVYTYQARVGGRE"
     gene            1348..2334
                     /locus_tag="ACFM8U_001868"
     CDS             1348..2334
                     /locus_tag="ACFM8U_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC6335049.1"
                     /translation="MRRKMLNVPKGNYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHIGSDVVSNVAQKNPL
                     FSLKRNSTNKLIPITESLNIGYPGFTQRLNALIFQYGIDDVNASADTTVVSSCAKIAK
                     PGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGRIAGEEGLFRFQLNDDGQ
                     WGNPQLLVKKINKMDIRYIYLLVENPGIPPTNSPRYIYGCPEDDDAGKEETFKYTDTF
                     NSSTNAVTPAGVEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2313..2906
                     /locus_tag="ACFM8U_001869"
     CDS             2313..2906
                     /locus_tag="ACFM8U_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC6335050.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCTANSTDLCIDNKGMEYKKGKGSVSKPPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2911..3408
                     /locus_tag="ACFM8U_001870"
     CDS             2911..3408
                     /locus_tag="ACFM8U_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC6335051.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNTVKQIILKNPQDSNDILNIKLTKFVSGYKMNPKIAEKYSVSGEFVDAPKSRA
                     NRALKSRAYRLVGVPKAGTGYTLSVWVNSVGDGYKCRDAASARAHLETLSADAGCEAF
                     SNRKK"
BASE COUNT          999 a          697 c          875 g          912 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gtcctatgtg tacacgaaaa caacaaggtt
       61 tcacgctaac agagctgctc atcgtgatgg tcattgcagc cattatggcg atgatagccc
      121 tccccaatat gagccaatgg attgcatccc gccgcattgc cagtcacgcg gagcggattg
      181 ccaacctttt gcgtttctcc aggggcgaag ccgtccggct caatctccct gtctatatct
      241 gtcctgttca agttaaaaaa gacggtacgc ccagcaataa atgtgactcc ggcaagaagg
      301 ggcagggaat gttggctttc ggcgacaaaa acggcaataa gggatatgac aatgatacgg
      361 cggatgttct tctccgcagt gtggtattga atgatgatat caatgataag cggattaatt
      421 atgccttcaa ccatatcgct ttcggtcaga ctcagccgac cgctgaccgt gtagtttgga
      481 cattcaatca aaacgggacg ttcggttatt ctaccaatca ggatctcacg aatacttcca
      541 aatttgttta ctccgacggt tatatccaaa tcgtgctgac agatgcgaga gcagtttcag
      601 atgccgataa gaaattccgt tcggcggtgg ttttgattga cagcagcggc agggtcgaag
      661 tttgccctag gggtgatcgg cgtactcggc gtactatgtg ccagtataaa taacagtttc
      721 agttttaaaa atgaatatga agaataatga ttgcttccgc ctgaaagatt cccagtccgg
      781 tatggcgctg atagaagtct tggttgctat gctcgttctg accatcggta ttttggcgtt
      841 actgtctgtg cagttgcgga cagtcgcttc cgtcagggag gcggagacac aaaccatcgt
      901 cagccaaatc acgcaaaacc tgatggaagg aatgttgatg aatccgacca ttgattcgga
      961 cagcaacaag aaaaactata atctttacat gggaaaccat catacactat cagttgtgga
     1021 tggcgatttt caggttggtg tcataaaaac taagacgcag ttggcagagg cacaattgaa
     1081 gagatttagt tatgagctga aaaatgcctt gccggatgcg gcagccatcc attacgccgt
     1141 ctgcaaggat tcgtcgggtg ctgcgccgtc attgtccgac agcggtactt tttctcaaaa
     1201 ttgcgatggt agtgcaaatg gggatacttt gattaaagta ttgtgggtaa atgattcggc
     1261 aggggattcg gatattgcac atacgaatct tgagacgaac ggcaacaata tcgtatatac
     1321 ctatcaggca agggtcggag gtcgggaatg agacgtaaaa tgctaaacgt accaaaaggc
     1381 aattatgatg gtatgaaggg ttttaccatt attgaatttt tggttgcggg catgctcagt
     1441 atgattgtcc tgatggcggt cggatcgagt tacttcacgt cccggaaatt aaatgatgcg
     1501 gcaaacgagc gtcttgctat tcaacaggat ttgcggaatg cggcaacatt aattgtccgc
     1561 gatgcaagaa tggcggggag cttcggttgt ttcaatatgt ctgagcatat cggaagtgat
     1621 gttgtttcca atgtggcgca aaaaaaccct cttttttcct taaaaaggaa cagtacaaat
     1681 aaacttatcc ccataacgga atctttaaat atcggatatc cgggttttac ccagcgcctt
     1741 aacgcattga ttttccaata cggaatcgat gatgttaatg caagcgccga tactaccgtc
     1801 gtcagcagct gtgccaaaat agcaaaaccg ggtaagaaaa tatctacctt gcaagaagca
     1861 aagagtgcat tacagattac taatgatgat aaacaaaatg gaaatatcac ccgtcaaagg
     1921 catgtggtca atgcctatgc ggtcggcagg attgccggtg aggaaggttt gttccgcttc
     1981 caattgaatg atgatgggca gtggggcaat cctcagttgt tggttaaaaa gattaataaa
     2041 atggatatac ggtatatcta tcttctcgtg gaaaatcctg gtataccgcc taccaattct
     2101 ccccggtata tttacggttg ccctgaagat gacgatgccg gcaaagagga aacattcaaa
     2161 tatacggata cattcaacag ctccacaaat gctgttacgc ccgccggggt ggaggtttta
     2221 ttgagtagcg gtactgatac caagattgcc gcttcttcag acaatcatat ttatgcttac
     2281 cgtatcgatg cgacaatacg cgggggaaat gtatgcgcaa acagaacact ttgacgggaa
     2341 tcccgacttc tgacggacag agggggtttg cactgtttat cgtgctgatg gtgatgatcg
     2401 tcgtggcttt tttggttgta actgccgcgc agtcttacaa taccgagcag cggatcagtg
     2461 ccaacgaatc agacaggaaa ttggctttgt ctttggccga ggcggctttg cgggaaggcg
     2521 aacttcaggt tttggatttg gaatatgata cggacagtaa ggttacattt agcgaaaact
     2581 gtggaaaagg tctgtgtacc gcagtgaatg tgcggacaaa taatgataat gaagaggctt
     2641 ttgacaatat cgtggtgcaa ggcaagccca ccgttgaggc ggtgaagcgt tcttgcactg
     2701 caaattctac cgacctgtgc attgacaata aagggatgga atataagaaa ggcaagggaa
     2761 gtgtcagcaa accaccacgc tatattatcg aatatttggg cgtgaagaac ggagaaaatg
     2821 tttatcgggt tactgccaag gcttggggta agaatgccaa taccgtggtc gtccttcaat
     2881 cttatgtaag caataatgat gagtaataaa atggaacaaa aagggtttac attgattgag
     2941 atgatgatag tcgtcgcgat actcggcatc atcagcgtca ttgccatacc ttcttatcaa
     3001 agttatattg aaaaaggcta tcagtcccag ctttatacgg agatggtcgg tatcaacaat
     3061 actgtcaaac agattatttt gaaaaatcca caggatagta atgatatcct caatataaaa
     3121 ctgacaaagt ttgtctcagg ctataagatg aatccgaaaa ttgccgaaaa atatagtgtt
     3181 tcgggggaat ttgtcgatgc gccaaaatca agggcaaaca gggcgttaaa atcaagggca
     3241 tacaggttgg tcggcgttcc gaaggcaggg acgggttata ctttgtcggt atgggtaaac
     3301 agcgtgggcg acggatacaa atgccgtgat gccgcttctg cccgagccca tttggagacc
     3361 ttgtccgcag atgccggctg tgaagccttc tctaatcgta aaaaataagg ttgttttgcc
     3421 gatgccgtct gaaaatcaat gttcagacgg catttttatg ggtatagtgg attaacaaaa
     3481 atc
//
LOCUS       DAXHSJ010000177         3465 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000177 DAXHSJ010000000
VERSION     DAXHSJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.177"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            207..1679
                     /gene="pyk"
                     /locus_tag="ACFM8U_001871"
     CDS             207..1679
                     /gene="pyk"
                     /locus_tag="ACFM8U_001871"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC6335052.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVKLLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVATGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            1989..3392
                     /locus_tag="ACFM8U_001872"
     CDS             1989..3392
                     /locus_tag="ACFM8U_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFC6335053.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKADILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAMAKQLWD
                     ANKLALLGYTPSEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVF
                     EKEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRM
                     NSLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSAR
                     FKNHADIIGLQYTYKFK"
BASE COUNT          893 a         1054 c          876 g          642 t
ORIGIN      
        1 aataaattta gcctaagtca taaaaccgtg taattttgcg ccaaaaaagg cggtttgcag
       61 gtaaaatatc aaactattaa tttaaatcat atttttacag aatattccgc cgcgttcatc
      121 aaatggacat caaaccggtt ccaaatttct gtaattttgt aacaaaatac cgcaaaacac
      181 ccgattgaga ccgaaaggac tttcatatga accagacaag ccgcgatctg acccgcatca
      241 gccacaacac taaaatcgtc gccacccttg ggccgggcag caacaacgtc aaactgttgg
      301 aagacatgat ccgcgtcggc ggtctgaacg tcgtccgctt caacttcagc cacggcacgc
      361 ccgaattcca tcaggaaaac gcccgcatcg tgcgcgaggc ggcaaaacgc gccggacagg
      421 aaatcgccat cattgccgac ctgcaagggc cgaaaatccg cgtgggcaaa atcgccggcg
      481 gcggcattga attgaacaaa ggcgaaacgc tggtactgga tgccgcgctc gaaggcgaag
      541 gcacgcgcga ggcggtcggt ttggactacc gcgatctgcc cgacgacgtt gccacaggcg
      601 atgtcttgtg gctggacgac ggcctgctga ccctgaccgt ggaatccgtc gaaggcagca
      661 ggattatcac aagggtggaa aacagccacg tcctgaaaag caacaaaggc atcaacaaac
      721 gcggcggcgg tctgtccgca ggcgcgttga ccgaaaaaga cttccgcgac ctgaaaaccg
      781 cgattgccat cggttgcgac tacctcgcca tcagctttgt gaaatccgcc gaagatttgc
      841 acatcgcgcg cgccaaagtc gaagaagaaa tgaagggcag cacggccgtg cgccccggtt
      901 tggtttccaa aatcgaacgc gtagaagcga ttgaaaacct tgatgaaatc atcctcgccg
      961 gcgacggcat tatggttgcg cgcggcgact tggcggtcga agtcggacac gccgccgtcc
     1021 ccgccctgca aaaacggatg atccgccgcg cccgcgagtt gcgccgcttc agcattacgg
     1081 cgacgcaaat gatggaatcg atgattacca accccgtgcc gacccgcgcg gaagtcagcg
     1141 atgtggcaaa cgcggtattg gacggcaccg atgcggtgat gtgttccgcc gaaaccgccg
     1201 tcggcgcgta tccgtttgaa accgtcagcc aaatggcgat tatctgcgcg gctgcggaaa
     1261 aagagcagga ttcgctcaac ggcgttgccg aacaggtcga gtatcccgaa gcggtcagca
     1321 ccaacctggc ggttgccggc ggtgcggtca gcgtggcgcg cgcggttcac gccaaagcca
     1381 tcgtcgccct gaccgaaagc ggttcgaccg cctttgaaat cagccgccac aacatcaccc
     1441 tgccgatttt cgcgctgacc ccgagcgttt ccgcccaacg ccgtatggcg atgtaccggg
     1501 gcgtgcgccc gctgattttg gcaaccagca ccgaccacga cacggcgtta aacgaagtcg
     1561 aaaccatgct ggtggaacac aacatcctgc attccggcga ccaatacatc attactagcg
     1621 gctcgcaaat gcgcgaatcc ggttcgacca atacgctgga agtgctgcgc gtcaaataat
     1681 ccgccctgag tggaaaatgc cgtctgaagc cgatgcccga ggcttcagac ggcatttttt
     1741 gcggcggcgc ggcggttcgg gcaaactctg aaaaaattgt gcaaatccgg caacatcgga
     1801 taaaatcgag tacctatact aaagcgaaac aaggcatttc cgactgcctt ttttatagtg
     1861 gattaaattt aaaccagtac ggcgttgcct tgccgtacta tctgtactgt ctgcggcttc
     1921 gtcgccttgt cctgattttt gttaatccac tatatttatc caccgtccgc ctttttacgg
     1981 aaaccgaaat gaccccttcc gcactgaaaa aaaccgtcct actgctcggc actgcctttg
     2041 ccgccgcatc cgcacaagcc tccggctacc acttcggcac acagtcggtc aacgcgcaaa
     2101 gcacggcaaa tgccgccgcc gcagaagccg ccgacgcatc gaccatcttc tacaaccctg
     2161 ccggcctgac caaactcgac agcagccaga tttccgtcaa cgccaacatc gtgctgccca
     2221 gcattcatta tgaggcggat tccgccaccg actttaccgg gcttcccgtc caaggttcga
     2281 aaagcggcaa aatcaccaaa accacggtcg cgccccacat ctacggcgca tacaaagtca
     2341 acgacaatct gaccgtgggc ttgggcgtgt acgtcccctt cggctccgcc accgaatacg
     2401 aaaaagattc tgtgttgcgc cacaacatca acaaactcgg tctgaccagc atcgccgtcg
     2461 aacctgtcgc cgcgtggaaa ctcaacgacc gccattcctt cggcgcaggc atcatcgccc
     2521 aacatacttc cgccgaactg cgcaaatatg ccgactgggg gattaagagt aaagcagata
     2581 tattgacggc aaaaccgccc aaacctaacg gtgtagccga agctgcaaaa attcaggccg
     2641 acggacacgc cgatgtcaaa ggcagcgatt ggggcttcgg ctaccaactg gcgtggatgt
     2701 gggacatcaa cgaccgtgcg cgcgtgggcg tgaactaccg ttccaaagtc tcgcacacgc
     2761 tcaaaggcga tgccgaatgg gcggcagacg gcgcaatggc gaaacagtta tgggatgcaa
     2821 acaaactcgc actgctcggc tacacgccaa gcgaaaaagc ccgcgttaaa atcgttacgc
     2881 ccgagtcttt gtccgtacac ggcatgtaca aagtgtccga caaagccgac ctgttcggcg
     2941 acgtaacttg gacgcgccac agccgcttcg ataaggcgga actggttttt gaaaaagaaa
     3001 aaaccgtcgt caaaggcaaa tccgaccgca ccaccatcac ccccaactgg cgcaacacct
     3061 acaaagtcgg cttcggcggt tcttatcaaa tcagcgaacc gctgcaactg cgcgccggca
     3121 tcgcttttga caaatcgccc gtccgcaacg ccgactaccg catgaacagc ctacccgacg
     3181 gcaaccgcat ctggttctcc gccggtatga aataccatat cggtaaaaac cacgtcgtcg
     3241 atgccgccta cacccacatc cacatcaacg acaccagcta ccgcacggcg aaggcaagcg
     3301 gcaacgatgt ggacagcaaa ggcgcgtctt ccgcacgttt caaaaaccac gccgacatca
     3361 tcggtctgca atacacctac aaattcaaat aaacggcccg ccgtttgaat gtaacaatgc
     3421 cgtctgaaac agatttccgt ttcagacggc attgttttag gctcg
//
LOCUS       DAXHSJ010000178         3437 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000178 DAXHSJ010000000
VERSION     DAXHSJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.178"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            374..826
                     /gene="dut"
                     /locus_tag="ACFM8U_001873"
     CDS             374..826
                     /gene="dut"
                     /locus_tag="ACFM8U_001873"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC6335054.1"
                     /translation="MNIEVEMKVLDERMADVVPVYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVVVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            904..2091
                     /gene="dapC"
                     /locus_tag="ACFM8U_001874"
     CDS             904..2091
                     /gene="dapC"
                     /locus_tag="ACFM8U_001874"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC6335055.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVVDVATCVKAAETIVS
                     LYR"
     gene            2254..3033
                     /gene="yaaA"
                     /locus_tag="ACFM8U_001875"
     CDS             2254..3033
                     /gene="yaaA"
                     /locus_tag="ACFM8U_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC6335056.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGQYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3116..3206
                     /locus_tag="ACFM8U_001876"
     tRNA            3116..3206
                     /locus_tag="ACFM8U_001876"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3150..3152,aa:Ser,seq:tga)
     gene            3284..3373
                     /locus_tag="ACFM8U_001877"
     tRNA            3284..3373
                     /locus_tag="ACFM8U_001877"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3318..3320,aa:Ser,seq:cga)
BASE COUNT          886 a          814 c          836 g          901 t
ORIGIN      
        1 ccgtcattcc cacgaaagtg ggaatctaga aatttaatgt tgcggcacta gccaaaaaaa
       61 ccgaaaccga caggtctaga ttcccgcctg cgcgggaatg acgaatccat ccgtacggaa
      121 acctgcacca cgtcattccc acgaacctgc atcccgtcat tcccacgaaa gtgggaatcc
      181 agttttttga gtttcagtca tttccgataa attgccttag tgtgtgcata atgttcgtct
      241 tcataacggg aagggcgggc attatacact ggcaacggat ttcaaaacaa aaccgatttg
      301 ccgtgtttca gcgtaaacac ggcttgtgta taatctccca tctttgaaac cggccgtatg
      361 caggagcaag acgatgaata ttgaagtaga aatgaaagta ttggacgaac ggatggcgga
      421 tgttgtccct gtctatgcaa cggagggttc tgcaggttta gatttgcgcg cctgtttgga
      481 tgaggaagtc gttttgcagc cgggtgaaac gtttcttgtg ccgacgggtt tggcaattta
      541 tttggcgaat cccgcatatg ccgccgtttt gctgccccgt tccggcttgg ggcataaaca
      601 cggcattgtt ttgggcaatt tggtcggttt gattgactcc gattatcaag gggaattgaa
      661 ggtgtcgtta tggaacagaa gcagcgaacc gtttactgtc aaaccgtttg agcgtatcgc
      721 gcagatggtt gtcgtgccaa tcgtgcaggc gcgtttcaaa cgtgtcgagg aatttgtcgg
      781 aagcagccgg ggtgagggcg gcttcggcag tacgggtttg cattgaatat aaaatgccgt
      841 ctgatgggtg tcgtcaggtt cggacggcat atcttcccaa tatgccggta atcggagaac
      901 attatgaata ccttactcaa ccaactcaaa ccctatccct ttgcccggct gcgtgaagcg
      961 atgcagggca tttccgcccc cgaaggtctg gaagccgtcc cccttcacat cggcgaaccg
     1021 aaacatccaa caccgaaagt cattacggat gcgctgaccg cctcattgca cgagttggaa
     1081 aaatatccgc tgactgcggg tctgcctgaa ctgcgtcagg cgtgtgcaaa ctggttaaaa
     1141 cgccgttatg acggcttaac agtggatgca gataatgaaa ttctgccggt tttaggcagt
     1201 agggaggcgt tgttttcttt tgttcaaacc gtgttgaacc ctgtttcaga cggcatcaaa
     1261 cccgcaattg tcagcccgaa tcccttttat cagatttacg aaggtgcgac acttttgggc
     1321 ggcggtgaaa tccattttgc caattgcccc gcgccgtctt tcaaccccga ttggcgcagt
     1381 atttccgaag aggtttggaa acgcaccaaa ctggtgttcg tctgctcgcc caacaacccc
     1441 agcggcagcg tgctggattt ggatggatgg aaagaagttt ttgatttaca ggataaatat
     1501 ggtttcatta ttgcctcgga tgaatgctat tccgaaatct atttcgacgg caacaaacct
     1561 ttaggctgcc tgcaggccgc tgcacagttg gggcgaagca ggcaaaaact gcttatgttc
     1621 accagtttgt ccaagcgttc caacgttccg ggcctgcgtt ccggttttgt cgccggcgat
     1681 gccgaactgc ttaaaaactt tctgctttac agaacctatc acggcagtgc aatgagcatt
     1741 cccgtgcagc gtgccagtat tgccgcctgg aatgacgaac agcacgttat tgacaaccgc
     1801 cgcctgtatc aggaaaaatt tgagcgcgtt attcccattt tgcaacaggt atttgacgtt
     1861 aaattaccgg atgcctcgtt ttacatctgg ttgaaagtcc ccgatggcga cgatttggca
     1921 tttgcacgca atttatggca aaaagccgct atccaagtat tgcccggacg ttttttggca
     1981 cgggataccg aacagggcaa tcccggggaa ggttatgtgc gtatcgcttt ggttgtcgat
     2041 gtcgcaactt gtgtcaaagc tgcggaaacc attgtttccc tatatcggta aagaataaaa
     2101 aatgccgtct gaacttttgt tcagacggca tttttcaata ttttacggtt gaatttgctg
     2161 taacggtatt tacttcactt ttaatctaat cggcttgccc gcaaacacgt ttaaatttaa
     2221 aatccccgtg tttgacacaa taccgagtgg attatgtttt ttgtcctttc ccctgcgaaa
     2281 aaccttaacg aaaaagaccc tgcccctgtc agcgagttta cccaacccga cctgctggca
     2341 gagtccgaca ttctaatgca gcagttgcgc gagcttgcgc cgcaacagat tgccgaactg
     2401 atgcacgttt ccgacaaaat tgccctctta aacgcgcagc gcaatgcaga atggcacacg
     2461 ccgtttacgc cggaaaacgc caaacaggcg gtctttatgt tcaacggcga tgtttacgaa
     2521 ggtatggatg caaacacatt ggatattgga cagatacgct atctgcaaaa ccatgtccgc
     2581 ctgctgtccg gtctgtacgg tcttcttcgc ccgttagacc tgatacagcc ctatcgtttg
     2641 gaaatgggga cggcatttgc caatttgcgc ggcaagaatt tgtatgagtt ttggggcgac
     2701 atcattacca accttttaaa tgacacgctt gcccaagcag gcagcaatac gcttgtcaac
     2761 cttgcctcac aggaatattt caagtccgtc aacacgaaaa aacttcgggc gcggctgatt
     2821 accccgatat ttaaagacga aaaaaacggt caatataaaa tcatcagttt ctatgccaag
     2881 cgcgcgcgcg gattaatggt gcgctatgcg gcagaacatc atattaccga ccctgaaatg
     2941 ctgaaaaatt ttaattacga aggctacgca ttcaatgacg cggcttcaaa tgaaagcgaa
     3001 tgggttttta tgcgttcgga acaaataaag tgaaaacaat aaattaagta ttttccgaaa
     3061 aaagtgcttg gcaaaatgta taaatttcat tattattcct aatcttcaag aagacggaag
     3121 cgtggcagag cggtttaatg caacggtctt gaaaaccgtc gagggttgat agccctccgt
     3181 gagttcgaat ctcaccgctt ccgccaatcc ttgaggtcaa aaaccaaata aaaatgaagt
     3241 agcataaagc atcggcattt ttatttggtt ttacttcatt atcggaaacg tggcagagag
     3301 gctgaatgca gcggactcga aatccgctga gggtgcaaat cctccgtggg ttcgaatccc
     3361 accgtttccg ccagaaaaca taaccgccct gattcggggc ggttcttttt tgttcaagat
     3421 tgagaccttt gcaaaat
//
LOCUS       DAXHSJ010000179         3435 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000179 DAXHSJ010000000
VERSION     DAXHSJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.179"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..815)
                     /gene="cysW"
                     /locus_tag="ACFM8U_001878"
     CDS             complement(<1..815)
                     /gene="cysW"
                     /locus_tag="ACFM8U_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC6335057.1"
                     /translation="MKPYSANPNLTEPRWLRVLLTAVALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIIT"
     gene            complement(1067..1903)
                     /gene="cysT"
                     /locus_tag="ACFM8U_001879"
     CDS             complement(1067..1903)
                     /gene="cysT"
                     /locus_tag="ACFM8U_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC6335058.1"
                     /translation="MLALKTPDVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     SGFWNTITEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPAGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGSGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            2244..2576
                     /locus_tag="ACFM8U_001880"
     CDS             2244..2576
                     /locus_tag="ACFM8U_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335059.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            2907..3416
                     /locus_tag="ACFM8U_001881"
     CDS             2907..3416
                     /locus_tag="ACFM8U_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC6335060.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          918 a          840 c          885 g          792 t
ORIGIN      
        1 ttggtgatga tgttctgcac cgccagcgtc gccagtgcta aaagtgccaa tacgccggag
       61 agggcgaatg cgccggtaaa gttgtattcg ttgtagaaga tttcgaccaa aagcgggacg
      121 gtgttggttt cgccgcgtat gtgtcccgat accacgctga ccgcgccgaa ttcgcccatc
      181 gcgcgggcgt tggtgaggat gatgccgtag agtaacgccc atttgatgtt gggcagggta
      241 acgcgccaaa acatctgcca gccgcttgcg ccgagtatca atgccgcctg ttcttcgctg
      301 tcgccctgag cctgcatcag cgggatgatt tcgcgtgcga caaaggggaa agtaacgaac
      361 agcgtcgcca gaataatgcc ggggatggcg aagataatct gtatgccttg cgcttcgagc
      421 cagccaccca atgccgtgtg cgcgccgaac aataagacga acattaaacc ggccaccacg
      481 ggcgatacgg aaaacggcaa atcgagcagg gtggtcagca actgcttgcc gcgaaaatca
      541 aaacgggtca gcagccacgc catcgccacg cccaagacgg cattgacggg aacgacaatc
      601 agcgcggtaa tcagcgtcaa tttgatggca gaccacgctt cgggatcgtt taaggatttc
      661 aggtacaaat cccaaccgcc ttttaaggct tcgtaaaaca cggcgacgag cggcacgacc
      721 agcatcagca gcagaaagcc cagcgcgaca gcggtcagca gcacgcgcag ccagcgcggt
      781 tcggtcaggt tgggattggc ggaatagggt ttcatggcgg tggtgttccc cggttggttt
      841 taaaatgttt ggaggtcgtc tgaaaaaccg ttttttgtgg ggcggattcg ttttcagaca
      901 accttttatt gattaaggga ataaaaacgt ctagctaccg tcattcccgc gcaggcggga
      961 atccaccgca gggcaacagg aaaacagaaa ataaataagg cagctgaaat tcaccaatgg
     1021 attcctgcct gcgcgggaat gacggtaaca ggtatttcag acgacctcaa cccttcgcgc
     1081 ccgaacgcct gcccaacgcc cactgcatca cgttcagcgc aaacagaatc acaaacgaaa
     1141 ccagcagcat aaacaacgcc accgccgacg cgccctgcac gtcgaactgt tccagcttgc
     1201 ccgtaataat cagcggcagg atttcagaaa ccatcggaat attgcccgcg ataaaaatca
     1261 ccgagccgta ttcccccgtt gcccgcgcga acatcattcc cgagccggtc aagagtgccg
     1321 gcgtgatttc cggcagcagc acgcggcgga acgtcgtaaa gcggtttgcg cccaaagtcg
     1381 ccgccgcttc ctcatattcg cccgataatt cctccaacac cggctgcacg gcgcggacga
     1441 taaagggcag gctgacgacg accagcgcaa tccaaatgcc ggcgggcgta aacgcgattt
     1501 tgatgcccaa aggctcgaaa aaacggccta tccaaccgtt gggcgcatac agggtcgcca
     1561 acgcgatacc cgtaaccgcc gtcggcagcg caaacggcaa atcgaccagc gcgttcgcca
     1621 gacccttgcc cgggaattca taacgcacca atacccacgc caccagcgtg ccgaacacga
     1681 cattggtcag catcgcataa aacgacatcc gcaaactcag ccagacggcc gccaagacat
     1741 tgggttcggt aatcgtgttc caaaatccgc tccagccgat ttccgccgcc ttcgccgcca
     1801 tcatcgcaaa cggcaagacc acaagcagcg acaggcacaa tacggtcaga ccaaggctga
     1861 gtttgaagcc gggcagtacg tcgggcgttt tgagcgctaa cataaaacaa tgctgaaaat
     1921 aaggaaaagg aaggactact ttaacgatgc cgtccgaaaa acggaaagaa tggaaagttt
     1981 ggtgcaaaga cgaatttgtt ataaagcggt tggcagtttc tcaagcgggc gcgatgtttt
     2041 aaaatatagt ggattaaatt taaatcagga caaggcgacg aagccgcaga cagtacaaat
     2101 agtacggaac tgattcactt ggtgctttag caccttagag aatcgttctc tttgagctaa
     2161 ggcgaggcaa cgccgtactg gtttaaattt aatccactat aacgccttgt tttgacggaa
     2221 aaccatcata taaaggaaca cttatgcaga ttttatcttt tcagccggac attgcggaac
     2281 gtatgctgga aggtacggaa ggcgagtcgg tcaacgaaaa cgcacaattc gtccgtacgg
     2341 acaacggcta ttggattgcg tggcatgaag gcgtagcggc actgcttgcg cccgatacgc
     2401 cgccgggcat tccctgtttt tgggtggaag gcgcggaaag ccttgaagag ttgtgcgtca
     2461 tggtggaacg cggcgagttt gacgaagtgg aagagtttga cggcgatgac gacgaatggc
     2521 tcgaaacggc acagggttgc gggcaccacg gcgacgcttg cgcctgcgga cattaaaggc
     2581 attgcaggct tgtcgcaagg gggcaaggct ttgccgtttt ttaaataatc ggtggattgc
     2641 atctgtttgc aatgggctga acgcttccct ttgtatttta ttgaataaaa aaacttatct
     2701 tggtattata attaaggcag catcaattat tttgggatgg caataaacgc aaagcattga
     2761 tttgcgccga ttgcagactt attatagcag gttgcggcgc ggacttaatg ggaattgttt
     2821 atagagcatg atggtctgaa acatcattaa ttaaaaatac cattaatccg atttatattt
     2881 atttcaattt caatggaaaa acatcaatga caatgatttt aagcatttta agcctgtttt
     2941 ttatcatcag actgttattt ttagccgtct ctattaaaca tgaaaaagcc ttgattgcca
     3001 aaggggcgaa acaatacgga aaaaccaatt ccacgctgct tgcggcagtt catacgcttt
     3061 attatttggc gtgttttgtt tgggtatggc tttctgacac tgcttttaat ggcatatcct
     3121 tgattggtac gctgacggtg atggcttcgt ttgtgatatt gtcattgatt attaagcagt
     3181 tgggggagat ttggacggtt aaaatctata ttttaccaaa tcatcaaatt aatcgttcgt
     3241 ggttgtttaa aacattccgc caccccaatt attttttaaa catcataccc gaactgattg
     3301 gcatcgcctt attatgtcaa gcgtggtatg ttttattgat tggcctgccc atttatttgc
     3361 tggtcttatt taagcgtatc cgacaagaag aacaggcgat ggcaacactt ttttaacccg
     3421 tttcatcaat tatag
//
LOCUS       DAXHSJ010000180         3349 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000180 DAXHSJ010000000
VERSION     DAXHSJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.180"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            117..1232
                     /gene="lst"
                     /locus_tag="ACFM8U_001882"
     CDS             117..1232
                     /gene="lst"
                     /locus_tag="ACFM8U_001882"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC6335061.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLNKSFDFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDGHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            complement(1396..1854)
                     /locus_tag="ACFM8U_001883"
     CDS             complement(1396..1854)
                     /locus_tag="ACFM8U_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6335062.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            1973..2794
                     /locus_tag="ACFM8U_001884"
     CDS             1973..2794
                     /locus_tag="ACFM8U_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6335063.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQVLADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            complement(2845..3291)
                     /gene="yciA"
                     /locus_tag="ACFM8U_001885"
     CDS             complement(2845..3291)
                     /gene="yciA"
                     /locus_tag="ACFM8U_001885"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC6335064.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKIEVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
BASE COUNT          797 a          716 c          956 g          880 t
ORIGIN      
        1 aatttaaacc actataatat tcggtaactg tcggaatatc tgctaaaatt ccgcattttt
       61 ccgccccggg acactcgggg cgtatgttta atttatcgga atggagtttt agggatatgg
      121 gcttgaaaaa ggcttgtttg accgtgttgt gcctgattgt tttttgtttc gggatatttt
      181 atacatttga ccgggtaaat cagggggaaa ggaatgcggt ttccctgctg aaggacaaac
      241 tcttcaatga agagggggag ccggtcaatc tgattttctg ctataccata ttgcagatga
      301 aggtggcgga aaggattatg gcgcagcatc cgggggagcg gttttatgtg gtgctgatgt
      361 ctgaaaacag gaatgaaaaa tacgattatt atttcaatca gataaaggat aaggcggagc
      421 gggcgtattt tttctatctg ccctacggtt taaacaaatc gtttgatttc attccgacga
      481 tggcggagct gaaggtgaag tcgatgctgc tgccgaaggt caaacggatt tatttggcaa
      541 gtttggaaaa agtcagcatt gccgcctttt tgagcactta cccggatgcg gaaatcaaaa
      601 cctttgacga tgggacaggc aatttaattc aaagcagcag ctatttgggc gatgagtttt
      661 ctgtaaacgg gacgatcaag cggaattttg cccggatgat gatcggagat tggagcatcg
      721 ccaaaacccg taatgcttcc gacgggcatt acacgatatt caagggtttg aaaaacatta
      781 tggacgacgg ccgccgcaag atgacttacc tgccgctgtt cgatgcgtcc gaactgaagg
      841 cgggggacga aacgggcggc acggtgcgga tacttttggg ttcgcccgac aaggagatga
      901 aggaaatttc ggaaaaggcg gcaaaaaact tcaacataca atatgtcgca ccgcaccccc
      961 gccaaaccta cgggctttcc ggcgtaacca cattaaattc gccctatgtc atcgaagact
     1021 atattttgcg cgagattaag aaaaacccgc atacgaggta tgaaatttat acctttttca
     1081 gcggcgcggc gttgacgatg aaggattttc ccaatgtgca cgtttacgca ttgaaaccgg
     1141 cttcccttcc ggaagattat tggctcaagc cggtgtatgc cctgtttacc caatccggca
     1201 tcccgatttt gacatttgac gataaagatt aatcgcatag caaatcaaaa tagaaaatgg
     1261 cggagtaagt aaggcaaaaa tcaggatatg gcgtattttt tgaattgaag ataatttctg
     1321 attgctttgc gtgtgctgaa atgacaaaga aaatgccgtc tgaaggcttc agacggcatc
     1381 gttctgtttc ggatgttatt ccggcgcgcg gaaactgtcg tggcaggatt tgcagcttgc
     1441 gccggtttcg ccgtaggcgg ctttgatttc atccagtttg ccggtttgtg ctgcggcgtt
     1501 gagtttttcg acggcggcgg cgaattttgt tttttcggct tcaaattttg caccatccga
     1561 ccaaacggcg ggcagggcgc ggccgttgcc ttgcggatcg gactcaaaaa gtgtgaacgg
     1621 tttcttgctg ctttcggcaa acgccgccgc cgcctgtttg aatttttcga catcgtaagg
     1681 ctcttcgcct ttgaccattt tgcccatacg cgagaagtcg ggcatcatag atttgaacgc
     1741 ggcggtgcgg ttttcggaaa tttcgccttt gggttgggaa ggtattccgc tgcctccgca
     1801 ggcggaaagg agcagtgtga tggcggcagc agcaaggctg atttgggttt tcatattgaa
     1861 tgtgtcctgt cgtggtggta tggttgttgt cattttcagc cggcgcgatg ccgtctgaag
     1921 agcgttatga tacctgaaac aggtttggga aacgagaaag aaaggaaaaa caatggctgt
     1981 tttaattacc ggtgcttcgg caggattcgg cgaagcgatg tgccgtgcgt ttgtcggggc
     2041 tggataccgc gttatcggtg cggcgcgccg tgcggacagg cttcaggttt tggcggatga
     2101 attgggtgct ttgttttacc ctttggaaat ggacgtgtcg cgacgcgagt cggtggaaaa
     2161 tgccttaaac ggcatccccg atgaattttc cgacatcgac tgcctcatca acaatgccgg
     2221 gctggctttg ggtttggaca cggcggacaa ggcggatttt gaagattggg aaacaatgat
     2281 tcaaaccaat gttttgggtt tgacgttcct gacgcgcaag attttgccgc aaatggtgga
     2341 acgcggcggc ggctatgtga tgaatttggg ttcgattgcg ggtaattatg cttatgccgg
     2401 cagcaacgtt tacggggcga ccaaggcgtt tgtgcgccag ttcagcctga atttgcgcgc
     2461 ggagttggcg gataagaaca tccgcgttac caatatcgag ccgggtttgt gcggcaatac
     2521 ggagttttcc aacgtgcgct tcaaaggcga tgacgagagg gcggcgggcg tgtatgaggg
     2581 tgtggaattt atccgccccg aagatattgc ggaaaccgca ttgtggctgt accgccggcc
     2641 ggcgcatatg aatgtgaaca cgattgaaat tatgcccgtg gcgcagactt ttgcaggaat
     2701 gaaagtgata aaaaaagccg tgcccgaagt gcgggaagac tttgaaaaac agagtatgtc
     2761 gctgttttcc cgcatcaggt cttggttcaa atgatacgga atgccgtctg aagacagttt
     2821 cagacggcat ttttacgggt atttttacgg agtaggcaat aagcccgaca atttggggtt
     2881 gccttctttc ggaatcgggc gcggattgcc ttccgcatcg atggcaacat aagtgaacac
     2941 cgcctcggtt acgaggtagc ggtcttcggt aacgcaatcg ttcatcaaag ttttcaccca
     3001 gacttcgatt ttaagctgga gggaagtatt gcccacgcgg acgcaatgcc cgtagcagca
     3061 gacgacgttg ccgaccttga ccgggcggat gaagttcatt tcctgaacgg cgacggtaac
     3121 gatgcgtccc cgcgcgattt ccgccgccaa tatgccgccg cccaaatcca tctgcgacat
     3181 aatccagccg ccgaaaatgt cttggttggg attggtatcg cgcggcatag cgacggtacg
     3241 caggagcagt tcgccttgag ggcgttggcg gttgccttct tcgtgctgca taatgtttcc
     3301 ttgttttatt gaaatatata gtggattaaa tttaaaccag tacggcgtt
//
LOCUS       DAXHSJ010000181         3343 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000181 DAXHSJ010000000
VERSION     DAXHSJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.181"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            60..689
                     /locus_tag="ACFM8U_001886"
     CDS             60..689
                     /locus_tag="ACFM8U_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC6335065.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGDKPYEYGKPLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            864..3238
                     /locus_tag="ACFM8U_001887"
                     /pseudo
     CDS             864..3238
                     /locus_tag="ACFM8U_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
BASE COUNT          896 a          873 c          826 g          748 t
ORIGIN      
        1 gggcaagtgc ggattttgcc cgccccgcat ccttatccct taccgacaaa ggaaaactta
       61 tgagtgaaac cgaaaatcaa gccctgacgt ttgccaaacg tctgaaggcg gataccacgg
      121 cggttcacga cagcgtggat aacctcgtta tgtctgtcca accgtttgtc agcaaagaaa
      181 actacatcaa atttttgaaa ctccaatccg ttttccacaa ggccgtcgac cacatctata
      241 aagatgccga attaaacaaa gccattcccg agctggaata catggcgcga tacgatgccg
      301 taacgcaaga ccttgcagat ttgggtgaca aaccttacga atacggcaaa ccgctgccgc
      361 atgaaaccgg caacaaagca atcggctggc tttattgcgc cgaaggatcc aatttgggcg
      421 cggcattttt gttcaaacac gcccaaaaac tcgattacaa cggcgaacac ggcgcgcgcc
      481 acctcgcacc ccatcccgac gggcgcggca aacactggcg cgctttcgtc gagcatctga
      541 acgctttgaa cctgactccc gaagccgaag cggaagccat ccaaggcgcg cgcgaagcct
      601 ttgcattcta caaagtcgtg ttgcgcgaaa ccttcggctt ggcagccgat gccgaagcac
      661 cggaaggaat gatgccgcac aggcactaaa aaataatcga accaaataaa caaggtctcg
      721 gcatagctgt ttgcagggac ctttaattac acggcgcggc tttgtttaca tggattactg
      781 tcttattaaa tattaatgat tatcataaaa tctattattc gctaaccgat ggatgaacaa
      841 tccatacatc ttgagttgat aatatgaaac cattacaaat gctccctatc gccgcgctgg
      901 tcggcagtat tttcggcaat ccggtctttg cggcagatga agctgcaact gaaaccacac
      961 ccgttaaggc agaggtaaaa gcagtgcgcg ttaaaggcca gcgcaatgcg cctgcggctg
     1021 tggaacgcgt caaccttaac cgtatcaaac aagaaatgat acgcgacaac aaagacttgg
     1081 tgcgctattc caccgatgtc ggcttgagcg acagcggccg ccatcaaaaa ggctttgctg
     1141 ttcgcggcgt ggaaggcaac cgtgtcggcg tgagcataga cggtgtaaac ctgcctgatt
     1201 ccgaagaaaa ctcgctgtac gcccgttatg gcaacttcaa cagctcgcgt ctgtctatcg
     1261 accccgaact cgtgcgcaac atcgaaatcg tgaagggcgc agactctttc aataccggta
     1321 gtggtgcatt gggtggcggt gtgaattacc aaaccctgca aggccgtgat ttgctgttgg
     1381 acgacaggca attcggcgtg atgatgaaaa acggttacag cacgcgtaac cgtgaatgga
     1441 caaataccct cggtttcggc gtgagcaacg accgcgtgga tgccgctttg ctgtattcgc
     1501 aacggcgcgg ccatgaaact gaaagcgcgg gcaagcgtgg ttatccggta gagggtgctg
     1561 gtagcggagc gaatatccgt ggttctgcgc gcggtattcc tgatccgtcc caacacaaat
     1621 accacagctt cttgggtaag attgcttatc aaatcaacga caaccaccgc atcggcgcat
     1681 cgctcaacgg tcagcagggg cataattaca cggttgaaga gtcttacaac ctgcttgctt
     1741 cttattggcg tgaagctgac gatgtcaaca gacggcgtaa caccaacctc ttttacgaat
     1801 ggacgccgga atccgaccgg ttgtctatgg taaaagcgga tgtcgattat caaaaaacca
     1861 aagtatctgc ggtcaactac aaaggttcgt tcccgataga ggattcttcc accttgacac
     1921 gtaactacaa tcaaaaggac ttggatgaaa tctacaaccg cagtatggat gcccgcttca
     1981 aacgcattac cctgcgtttg gacagccatc cgttgcaact cggggggggc gacaccgcct
     2041 gttgttcaaa acctttgcca gtcaacgtga gtttgaaaac ttaaaccgcg acgattacta
     2101 cttcagtggt cagatatctc gtaccaccag ctcgattcaa caccctgtga aaaccaccaa
     2161 ctacggtttc tcgctgtctg accaaatcca atggaacgac gtgttcagca gccgcgcagg
     2221 tatccgttac gaccacacca aaatgacgcc tcaggaattg aatgccgact gtcatgcctg
     2281 cgacaaaacc ccgcccgcac cgaatactta cagcggctgg agcggtttcg ccggtttggc
     2341 ggctcaactg aatcaggctt ggcgtgtcgg ttacgacatt acttccggct atcgcgtccc
     2401 caatgcgtcc gaagtgtatt tcacttacaa ccacggtgcg ggcacatggc tgcctagtcc
     2461 taatctgaaa gccgagcgca gcaccaccca caccctgtct ttgcagggac gcggtgaaaa
     2521 aggtacgctg gatgccaatc tgtatcaaag caactaccgc aatttccttt cggaagaaca
     2581 gtttatacaa cttgtgcgtg ataaatattg tgatgaagat aatgctctag caggttattg
     2641 taaactctac cgaaatcagc tttattggca tatggaaaat atcgacaagg ccagaatccg
     2701 cggcatcgag ctgacaggcc gtctgaatct ggacaaagta gcgtcttttg ttcctgaggg
     2761 ctggaaactg ttcggctcgc tgggttatgc gaaaagcaaa ctgtcgggcg acaacagcct
     2821 gctgtccaca cagccgctga aagtgattgc cggtgtcgat tacgaaagcc cgagcgaaaa
     2881 atggggtgtg ttctcccgcc tgacctatct gggcgcgaaa aaggccaaag acgcgcaata
     2941 caccgtttat gaaaacaagg gctggggtac gcctttgcag aaaaaggtaa aagattaccc
     3001 gtggctgaac aagtcggctt atgtgttcga tatgtacggc ttctacaaac cggctaaaaa
     3061 cctgactttg cgtgcaggcg tatataatgt gttcaaccgc aaatacacca cttgggattc
     3121 cctgcgcggc ctgtatagct acagcaccac caactcggtc gaccgcgatg gcaaaggctt
     3181 agaccgctac cgcgccccaa gccgtaatta cgccgtatcg ctggaatgga agttttaatc
     3241 tggtattatt gaattaatcg ccttgttgaa aattaaagcc gtccgaattg tgttcaagaa
     3301 ttcattcgga cggcttttta ccgtaaatct gtgtgatggg gtt
//
LOCUS       DAXHSJ010000182         3341 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000182 DAXHSJ010000000
VERSION     DAXHSJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.182"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..188
                     /locus_tag="ACFM8U_001888"
     CDS             <1..188
                     /locus_tag="ACFM8U_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335066.1"
                     /translation="IFVNPLYRHSRKNKKIKNTKLKYRHSRAGGNLGLSVRKLIGKNG
                     FSNPETPDSCFRGNPVF"
     gene            complement(506..751)
                     /locus_tag="ACFM8U_001889"
     CDS             complement(506..751)
                     /locus_tag="ACFM8U_001889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335067.1"
                     /translation="MPSELQTAFPASQLQRERTHTAIPAKNRKSKKKPKIRHSRAGGN
                     PIRPVSVFFEFQVTSKPPFPQKRESRNSKLQEFIKND"
     gene            complement(764..1348)
                     /gene="rnhB"
                     /locus_tag="ACFM8U_001890"
     CDS             complement(764..1348)
                     /gene="rnhB"
                     /locus_tag="ACFM8U_001890"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC6335068.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQTLF"
     gene            complement(1417..3312)
                     /gene="mnmG"
                     /locus_tag="ACFM8U_001891"
     CDS             complement(1417..3312)
                     /gene="mnmG"
                     /locus_tag="ACFM8U_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC6335069.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNADMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTYEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGITPAAVALLMVHLK
                     RGFKDAK"
BASE COUNT          609 a          798 c         1011 g          923 t
ORIGIN      
        1 tgatttttgt taatccacta tatcgtcatt cccgcaaaaa caaaaaaatc aaaaacacaa
       61 aactgaaata tcgtcattcc cgtgcaggcg ggaatctagg tctgtcggta cggaaactta
      121 tcgggaaaaa cggtttttcc aaccctgaga ctccggattc ctgttttcgc gggaatccgg
      181 ttttttgagt ttcagtcatt tttgataaat tcttgcagct ttgagtttct agattcccgc
      241 ttttgcggga atgacgcgga aaagttgctg tgatttcgga taaattttcg tcacgcttaa
      301 tttctgtttt atccgataaa tgcctgcaat ctaaaatttc gtcattcccg caaaaacaaa
      361 aaatcaaaac agaagcctaa aatttcgtca ttcccgcgaa ggcgggaatc taggtctgtc
      421 ggtacggaaa cttatcgggt aaaacggttt tgccagccct gagactccgg attcctgttt
      481 tcgtaggaat ccggtttttt gagcttcagt catttttgat aaattcttgc agctttgagt
      541 ttctagattc ccgcttttgc gggaatggcg gtttggaagt tacctgaaat tcaaaaaaaa
      601 cggaaaccgg acggattgga ttcccgcctg cgcgggaatg acggatttta ggtttctttt
      661 ttgattttct gtttttcgcg ggaatggcgg tatgggttct ttctctttga agttgcgatg
      721 ctggaaatgc cgtctgaagt tcagacggca tttttgtgcc ggtttaaaac agcgtctgct
      781 gcgcgagcag gtttctgacg ggggcgaagt cgcggcgatg ttcgggcagc acgccgtatt
      841 gtttgagggc ttccagatgc tgcttcgtgc cgtaaccttt gtgtttgtcg aaaccgtatt
      901 ggggatggcg ttgcgccagt gcgtacattt ccgcatcgcg tgcggtcttt gccaaaacgg
      961 atgcggcgga gatttcgatg attttgctgt cgcctttgac gacggcttcg gcagggatac
     1021 ctaaatgttc tggaatgcgg ttgccgtcga tgaatattct ttcgggacgc acagccaaac
     1081 cgtcaacggc gcgtttcatg gcgagcatgg tagcgtgcag gatgttgagg ctggcgattt
     1141 cttcgggcgt ggcggcggcg acgtgccacg cggccgcctg attttttatc atttcggcaa
     1201 gcgcgtcgcg ttttttctcg ctgagttttt tggagtcggt cagtccgggc aggtcgaatg
     1261 tttccggaag gatgacggcg gcggcaaaca cgctgccgac taaaggcccg cgtcctgcct
     1321 cgtccacgcc ggcggtcagt atgtgcatga tgtttcctgt cgggatagta ggaatgccgt
     1381 ctgaaaaggg tttcagacgg catcgtaccg atgtgtttat ttcgcgtctt taaacccgcg
     1441 cttcaaatgc accatcagca atgccactgc cgcaggggtt atgccggaaa tgcggctggc
     1501 ttgtccgacg gtttcgggtt tgtgctgatt gagcttttgc tgcacttctg ccgacaagcc
     1561 tttgactttg ccgtaatcga tgccgtcggg cagttttaag gtttcgatgt cgcggcggct
     1621 gtcgatttct tcgttttggc ggtcgatata gccttggtat ttgacttgga tttcgacttg
     1681 ctcgatgact tcggcggaga ggttttcaga cggcatcgcg ccttcgagcg tcatcagcgc
     1741 ggcgtagtcg aggtttgggc ggcgcaggag gtcgtgcagg ttggcttcgc ggctgagttt
     1801 ttgtccgaac acgcggattt gttcgtcttc agcgagtttt tgcggcgtgt accacgttgt
     1861 tttcaaacgt tggatttcgc gttcgatggc ttcgcgtttt tcgttgaaca tacgccattg
     1921 cgcttcaccc accaagccga ttttgtagcc gtcttcggtc aggcgcatat cggcgttgtc
     1981 ttccctgagt tgcaggcggt attcggcgcg gctggtgaac atgcggtaag gttcgttcac
     2041 gcctttggtg atgaggtcgt ccaccaatac gccgaggtag gcttgttcgc ggcgcagcag
     2101 gagcgggtct tgttcgcgca catattgcac ggcgttcgcg cctgccaata aaccttgcgc
     2161 ggcggcttct tcgtagccgg tcgtgccgtt gatttgcccg gcgaaaaaca gtccctcaat
     2221 ggttttggtt tcgaggctgg ctttgaggtt gcgcggatcg aagtagtcgt attcgatggc
     2281 gtagccgggg cgcaggatat gggcgttttc caaacctttc atgctgcgta caagcgcgat
     2341 ttggatgtcg aacggcaggc tggtggagat accgttagga tagtattcgt acgtagtcag
     2401 gccttcgggt tcgaggaaaa tctggtggct gtctttgtcg gcgaagcggt tgattttgtc
     2461 ttcgatagac gggcagtagc gcggaccgac gccttcgatt ttgccggtga acatggggct
     2521 gcggtcgaag cctgagcgga tgatgtcgtg ggtttgcgtg ttggtatgcg taatccagca
     2581 ggatacttgg cgcgggtgca tatcggcgtt gccgcgtacg gacatgacgg gaacgggcgt
     2641 gtcgccgggc tgttcggtca gttgggagaa gtcaatcgtg cgtccgtcga tgcgcggcgg
     2701 cgtgccggtt ttcagacggc cttgcggcag gttcaattcg cgcaaacgtc cgcccagcga
     2761 tttggcggcg gggtcgccgg cgcgtccgcc ctcgtagttt tccaaaccga tgtggatttt
     2821 gccggacaaa aacgtgcctg cggtcaacac cacggcgcgt gctttaaact ccacgcccat
     2881 cgcggtgatt acgcctgaaa tgcggtcgcc atcgagcgtt acgtcttcga cggcttgttg
     2941 gaaaaggtcg aggttttctt ggttttccaa catttcgcgg atggcggctt tatataggat
     3001 acgatccgcc tgcgcgcgcg tggcccgtac tgccgcgcct ttgctggcgt tcagacggcg
     3061 gaactggatg ccggatttgt cggttgccaa cgccatcgcg ccgccgagcg cgtcgagttc
     3121 gcgcaccaaa tgccctttgc cgatgccgcc gatggaaggg ttgcacgaca tttgtccgag
     3181 cgtttcaata ttgtgtgtga gcaaaagcgt ctgcgcgccc atacgggcgg cggcgagcgc
     3241 ggcttctgtg ccggcgtgtc cgccgccgac gacgataacg tcgtaggttt tggggtaaat
     3301 catgtgggtc atgtgtgtat tgcctgacgg tgtttcagac g
//
LOCUS       DAXHSJ010000183         3251 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000183 DAXHSJ010000000
VERSION     DAXHSJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.183"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            411..1034
                     /locus_tag="ACFM8U_001892"
     CDS             411..1034
                     /locus_tag="ACFM8U_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC6335070.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGTLLLSPLAQAGAQREETLADDV
                     ASVMRSSVGSINPPRLVFDNPKEGERWLSDMSARLARFVPDEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1145..2527
                     /locus_tag="ACFM8U_001893"
     CDS             1145..2527
                     /locus_tag="ACFM8U_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6335071.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2531..3055
                     /gene="ssb"
                     /locus_tag="ACFM8U_001894"
     CDS             2531..3055
                     /gene="ssb"
                     /locus_tag="ACFM8U_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC6335072.1"
                     /translation="MLLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          678 a          863 c          968 g          742 t
ORIGIN      
        1 gaaaacctgt gtttgggttt cggctgtcgg gagggaaagg aattttgcaa aggtctcaag
       61 atatagtgga ttaaatttaa atcagtacgg caaggcgagg caacgctgta ctggtttaaa
      121 tttaatctac tatatttccg cctgctgccg cccgaaaagc gtgatgcgct gatggtgctg
      181 ctgcataatc cggacgacgg ataaccggtg aaaatgggaa atgcccgggc ggtgttgcct
      241 gtgcctgttt ggacaagcgt tcttgagggc ggtaaaatca aggtttgccc gaaaagggca
      301 gggcgatatg ccgcgcttgg attttgtgcg cggtttttcg cgttggaagc aatcatgtct
      361 gtttgaatat ttaatgccgt ctgaaacgtc cggacgcgcc cccgttccct atgaaaaaac
      421 cgaccgatac cctacccgtt aatctgcaac gccgccgcct gttgtgtgct gccggcacgc
      481 tgttgctcag cccgctggca caagccggcg cgcaacgtga agaaacgctt gccgacgatg
      541 tggcttccgt gatgaggagt tccgtcggca gcataaatcc gccgaggctg gtgtttgaca
      601 atccgaaaga gggcgaacgt tggttgtccg acatgtcggc acgtttggca aggtttgtcc
      661 ccgatgagga ggagcggcgc aggctgctgg tcaatatcca gtatgaaagc agccgggccg
      721 gtttggatac gcagattgtg ttgggactga ttgaggtgga aagcgcgttc cgccagtatg
      781 caatcagcgg cgtcggtgcg cgcggtctga tgcaggttat gccgttttgg aaaaactaca
      841 tcggcaaacc ggcgcacaac ctgttcgaca tccgcaccaa cctgcgttac ggctgtacca
      901 tcctgcgcca ttaccggaat cttgaaaaag gcaacatcgt ccgcgcgctt gcccgcttta
      961 acggcagctt gggcagcaat aaatatccga acgccgtttt gggcgcgtgg cgcaaccgct
     1021 ggcagtggcg ttgattttga acccgcgccg caaccgaaat acggcgaatc ctgtataatc
     1081 cgaaaatctg ttcactggaa gttcagacgg cattgcaact gttgatgccg tctgaaaaaa
     1141 tatgatggca agagacaacc gcatccaaat gtttccgcac gaatggcgcg ccagtacgac
     1201 gctttccggc gtgtacgcgc tgcgtatgct gggtatgttc ctcgtgctgc ccgtattggc
     1261 ggtgtatgcc gcctcgctgc ccggcgcgga aggcaacaaa acgctggtcg ggctggcaat
     1321 gggcatttac gggctgacac aggctctgct gcaactgcct ttgggcatcg cttccgacaa
     1381 gttcgggcgc aagaaaacca tttatgtcgg gctgattgtg tttgcggcgg gcagctttct
     1441 tgccgccgcc gccgatacgc tgcccatgct ggtcgccgca cgcgccatac agggtgcagg
     1501 ggcggtcagt gcggcggtta ccgcgctgct ggcggatttg acgcgcgacg gcgtacgtac
     1561 ccgcgcaatg gcgatgatcg gtttgagtat cggtttgacg ttttcggtca gcctcgtcgt
     1621 tgcccccgtg attgccgacg cggtcggcgt tcgcggactg tttatgctga ccggcattct
     1681 gaccgtcatc agcatcggcg tggtggcgtg gatgactccc gatcccgaag tttccaagct
     1741 gcacgaagat acgcaggcgc agccttcgcg cataggcgaa gttttgaaaa accgtaggct
     1801 gctgacgctc gatttcggca ttttcgccct gcacgccgcg caaatggcat tgtttaccgc
     1861 gctgcctttc gcgatgaccc agctcggttt ggaaaaaatc cagcactgga aagtctatct
     1921 gccctcgacc attacgggat tggtggtaat ggttccgctg attatcgtcg gtgagacgcg
     1981 caacaagctc aaacaggttt ttatcttggg tatcgcctgt attgcggcgg cgcagctcgg
     2041 tttgctgtcc ggtatgcgct cggtaggctt gattaccgct tatttggttg tttactttat
     2101 cggttttaat gtgttggaag cgagcctgcc gtctatggtt tccaaaatcg cgccgtccga
     2161 cctgaagggt acggcgatgg gcgtgtacaa cacgatgcag tcgctcggac tgtttgccgg
     2221 cggcgcggca ggcggtttgc tgtttcaaaa atacggcttt tccggcgtgt ttgccttttg
     2281 cagcatattg atgctgctgt ggctggtgat tgccgtttta tcgcctgcgc ccaagcccgt
     2341 caaaaacctc agttaccctg tcggcggcgt gtggcaggtc aatcaggaag ggttataccg
     2401 cgccttgtcg gagcttgagg gcgtggaaga catcggtttc agtttcgacg ggcagaccgt
     2461 ctatctcaaa gtgttgcaga agggtttcga tcaggctgcc gctgaaaaaa tcatcacagg
     2521 agtttaaaaa atgttattga acaaagtcat ccttatcggc cgcctcggac gcgatcccga
     2581 agtgcgctat atgcccaacg gcgaggcggt gtgtaatttc agcgtcgcca ccagcgaaac
     2641 atggaacgac cgcaacggcc aacgtgtaga gcgcaccgag tggcacaaca tcacgatgta
     2701 ccgcaaactg gcggaaattg ccgggcaata cctcaaaaaa ggcgggctgg tttatttgga
     2761 aggcagaatc caaagccgca aataccaagg taaagacggc atcgagcgca ccgcttacga
     2821 tattgtcgcc aacgaaatga aaatgttggg cgggcgcaat gaaaacagcg gcggcgcacc
     2881 ttacgaggaa ggttacggtc agagtcagga ggcttaccaa cgccccgcgc agcaaagccg
     2941 gcagcctgcc cccgacgcgc cgtcccatcc tcaagaagca ccagccgcgc cgcgccgcca
     3001 acccgtgccg gccgccgccc cggtcgagga tattgacgac gacatcccgt tctgaatttt
     3061 acggcagcac atcctcgcgg agggaaatca taaaggacgg agaaacctta aaccttacgg
     3121 ggcagggttt ctcttttttg catttgtgcc ggcttgttgc tgccccctgc gttgggcgga
     3181 acgttgcgat tgcgccgttc tggcgttttg aggcagtgat gcctgccgca cgtccccgct
     3241 gttttcaggc g
//
LOCUS       DAXHSJ010000184         3236 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000184 DAXHSJ010000000
VERSION     DAXHSJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.184"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            107..1060
                     /locus_tag="ACFM8U_001895"
     CDS             107..1060
                     /locus_tag="ACFM8U_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC6335073.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1177..1500)
                     /locus_tag="ACFM8U_001896"
     CDS             complement(1177..1500)
                     /locus_tag="ACFM8U_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335074.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1673..3034)
                     /locus_tag="ACFM8U_001897"
     CDS             complement(1673..3034)
                     /locus_tag="ACFM8U_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC6335075.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASQEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVEGT
                     DWE"
BASE COUNT          759 a          943 c          780 g          754 t
ORIGIN      
        1 ttagagaatc gttctctttg agctaaggcg aggcaacgct gtactggttt aaatctaatc
       61 cactatagat taaaatcaca caatatccaa ctattcggac cttcatatga cacataccgt
      121 ccacctgcat ttagaagaaa ccgacaactt ggcgttgcag cgtctgtgcg gttcttttga
      181 caacaacctt gatttacttg ctaaagcact cgatatccac atcagccgcc gttttgaaca
      241 tttcactttc aacggcgcat ttgcacacgc cggcaaacgc gcactgctca aactcttgga
      301 aacggcgcag acgcgcgacc taaacgacgg cgacatcagg cttgccgccg tcgaagccca
      361 aaccgaagat gccggtcatc aagaaaaaaa ccatgaccac gcctattatt tccgcaccaa
      421 gcgcggcagc atcggcggca gaacgccacg gcagaacggc tatatccgcg ccctactcaa
      481 ccacgacatc gtattcggtc tcgggccggc aggtacgggc aaaacctatc tcgccgtcgc
      541 cgccgccgtc gatgcgatgg aaaaacacca agtcgaacgc atcattttag tgcggcctgc
      601 cgtcgaagcc ggcgagaaac tgggttttct gcccggtgac ctgacccaga aagtcgatcc
      661 ctaccttcgt ccgctttatg atgccctcta tgacctgatg ggctttgacc gtgtaaccaa
      721 gctgattgaa aaaggcctga ttgaaatcgc cccgctcgcc tatatgcgcg gcaggacgct
      781 caacggcgca tacatcatcc tcgacgaagc gcaaaacacc acgcccgaac aaatgaaaat
      841 gttcctgacc cgcatcggct tcggcgcgaa agccgtcatt accggcgaca ccagccaaat
      901 cgacctgccc aaaaacatca aatcgggatt aaaagatgcg cgtgagaaac tgcacaacgt
      961 ggaagggctg tatttccaca cctttaccgg cgaagacgtt gtccggcatc ctttggtgca
     1021 aaaaatcgtc gaagcctacg aatcggcaga acacgactga cattccaatg ccgtctgaaa
     1081 cccgatgcat ggaaaatcgg gcagagaaaa acagatacgg aagccaagct ccgtatccgt
     1141 atccgtatcc gtttctttat ccgtccaacc gatacatcaa tcgaattttc tgcatcggat
     1201 tgcctcatct tcgcaaaaca ttgccgaagt ttcgcggttg cacgcctgac gcactttctg
     1261 ccgcgccacg ccttcattcc gatcgcttgc ctcaaacgtc ttctgaaaag gcgtaacgct
     1321 gcatacatac atggtggaat ttgaaccgaa aaggtcgatt ttgacggtat tgctgccgct
     1381 ttccaatacg gcgacacggt gttccaactg cgccagacgt gcttccaaat cgccgattct
     1441 gtcggcaaat gcaggcatgg cggcacacgc ggtcaaaatg cccaaaatac attttttcat
     1501 caatttttcc ttgtttcttg aaccctgccc acccggacat cctttttccg agaggcaggg
     1561 ttagacttta tttacgggga tgaagcccct tacaaatcag gataatacat agaacggcac
     1621 tttatgcttc atcccatgtg ttgaaacttc attgctcctt gtctgttgac ccttattccc
     1681 aatccgttcc ctcaaccttc ggcttgggtt tgcgcctgcg acggcggcgt tttttcgcct
     1741 gtccttcgtt tttttcgtgc cgggcagcag cctcgttttt ggtcatttcc gtccgttgtt
     1801 cctgcgatgc cgtctgaaac gccgtccacc actcggcaag ggcgcggtcc gcattgccgg
     1861 tttcggcgcg caagagcagg aaatcatagg cggcacggaa acgcgcctgt gcaaacagtt
     1921 tgtgcggcct tgcgcctttg cggttttcaa actgcggctg gaacatccaa atttcgcgca
     1981 tcgtggcgga aaagcgttgc ggtacgcccc aaccgcgttc gacggtttcg cgcatcgtat
     2041 tgattgcatc ggacagggcg ggcacgggtt tcaaaccctg ttgcagattg cttttccaat
     2101 ggcgttccaa ctcgggccac atcagggccg ccagtacgaa accgaccgaa accgatttgt
     2161 cggcacgcag ccgctcatcg gtatttttca gggcaagcac cgtcattttt ccggcgatgc
     2221 cgtctgaaac gcgcaaggca ttgagcagcg gatggatgtc gtccggtatg tcaaatccgt
     2281 tcaaacgttt cagacactcg cgcgcgtgcc ctgaaaacag cagtttcata atttcgtcga
     2341 acagccgcgc taccggttcg tgcttcagac ggcatatcga ttcggcaatc ggtgcggcgg
     2401 tttcttccga cagatcaaag cccaatttgc tcgacaggcg gatggcgcgc aaaatcctga
     2461 cagggtcttc ctgatagcgt tcggcggcat caccaatcat aaccagcctg cgggcggcaa
     2521 catcggcaat cccgttgtgg aaatccaaaa tctcttcttt ttcaggatcg taatacaagg
     2581 cattgcaggt aaaatcgcgc cgcatcgcat cttcttcgat gctgccgtag gtgttgtcct
     2641 tcatgattct gccgtgcgcg ttctgatgaa ccttcgcacc gccgcgaaac gtcgttactt
     2701 cgataatctc cgcaccgttc atcacatgga caatctgaaa acgcctgccg atgatgcggc
     2761 tgcggcggaa gagtttgtgc acctgttcgg gcgtggcatc ggttgcgaca tcgaaatcct
     2821 tgggttcgat gccgagcagc aggtccctga ccgcgccgcc gaccacataa gcctgaaacc
     2881 ccgccccttt caggcggcgt atgacgtttt cggcggcaaa gctcaacatt tcggcacgga
     2941 tgttgtgtct ttcggcagga atgatcgttt tactttccgc ttttttactg ctccgaccgg
     3001 aaggcagcat cttattcaac cattttttca gcataaatat tctaacccga agagcggcat
     3061 ctccacgggc aaccgtgttc agacggcatc aggcggtacg attccgcaag atgccgtaac
     3121 cataaacaaa aggcgtagaa actacgccgt ccgaatcacg ccgccctcgc ggcggcggca
     3181 acgcgccatt ataacagatt gccctttaaa aaaatcagac cctgcttttg tggcgg
//
LOCUS       DAXHSJ010000185         3210 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000185 DAXHSJ010000000
VERSION     DAXHSJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.185"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..593)
                     /locus_tag="ACFM8U_001898"
     CDS             complement(<1..593)
                     /locus_tag="ACFM8U_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6335076.1"
                     /translation="MDRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNGDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPLADFLELLSLCRDIHALTEGAFDPTVQVLWKLYADYFAAHPDAETPPPQN
                     SVDEALKRVGFGKVSFDDREIRFAEKGMGLSLNGIAQG"
     gene            complement(604..1398)
                     /locus_tag="ACFM8U_001899"
     CDS             complement(604..1398)
                     /locus_tag="ACFM8U_001899"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC6335077.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQTAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1782..3116
                     /gene="gdhA"
                     /locus_tag="ACFM8U_001900"
     CDS             1782..3116
                     /gene="gdhA"
                     /locus_tag="ACFM8U_001900"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC6335078.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
BASE COUNT          838 a          912 c          770 g          690 t
ORIGIN      
        1 ccttgcgcga tgccgttcaa ggacaaaccc ataccctttt cggcaaaacg gatttcacgg
       61 tcgtcaaacg agactttgcc gaagccgacc cgtttcaggg cttcatccac gctgttttga
      121 ggcggcggcg tttccgcatc gggatgagcg gcaaaataat cggcatacag tttccacaaa
      181 acctgaaccg tcggatcgaa tgccccttcc gtcagcgcgt gaatatcgcg gcacaggctc
      241 aacagttcca aaaaatccgc cagcggcgaa gtcagataac cgtcacggtt cagacggctg
      301 atcaggctgt cttcacggta aaggctgaac attttttcca aacgcgccac ttccgccaaa
      361 accttgttga ccaaatccgc cgcacgcctg tcgtccacac cgaacagacg gagctccgca
      421 ccggaaccca gtgcgacacc tttccagaaa aacacatttt cattgcgttt ttcatccccg
      481 ttgcgttttt catcatcggc ggcaaaagga ttcggcagga aagaaaccgc cgcgcccgcc
      541 gccgcaacgg cggcaaccgt cagaaaacgc ctgcgcccga aatgcctgtc catatacccc
      601 tcctcagacc gacactgccg ccttgatgtg cggatggggg tcgtagcctt ccaactcgaa
      661 atcttcaaac ttgaaggaaa acaaatcttt gacttcagga ttgattttca tcactggcaa
      721 ggcgcgcggt tcgcgttcca actgcaatgc ggtctgctcg aaatggttgc ggtacaaatg
      781 cgcgtcgcca aacgtatgga caaactcgcc cgcctccagc ccgcacactt gcgccatcat
      841 catggtcaac aatgcgtagc tggcaatatt aaacggcaca ccgaggaaaa tatccgcgct
      901 gcgctggtaa agctggcagg acagtttgcc gtcggcaacg taaaactgaa acagcgcgtg
      961 gcagggcggc aaggccattt catcgaccaa agccggattc cacgccgata caatcaggcg
     1021 gcgcgagtcg gggtttttct taatttgttc caccacattg gcgatttggt cgatatgcct
     1081 gccgtcgggc gcgggccagt tacgccactg gtagccgtaa accgggccta agtcgccgtt
     1141 ttcgtccgcc cactcgtccc aaatggaaac attgttgtcc tttaggtatt tgatattggt
     1201 atcgcctttg agaaaccaaa gcagctcgtg gataatcgag cgcaaatgca gctttttggt
     1261 cgtcagcagc ggaaaacctt tgcccaagtc aaaacgcatc tgataaccga atacggagcg
     1321 cgtgcccgta ccggtgcggt ctgatttgtc cgtaccgttg tcgaggacgt ggcgcatcaa
     1381 gtccaaatag gctttcatag cagtctttca tcaaattaaa cggcgcatat tgtaacattt
     1441 ccggataatg cccaaaacac ggatacaggc aggcaggatt gttggcaatt tcagtccttt
     1501 tccacagtaa aacccagtgg gaaaacaaaa ttaccttgat tggaatcaaa aaatctagtt
     1561 taattactta gaataaaatt tcaataatat ttttatttac gaaattaatt tatgattatt
     1621 gattaattat cggcaacaaa catcaaacat cgaaaacatg gaaaaaataa tgtcaacaat
     1681 ttttgccaaa tcgggcttgg catcagaaaa aaataggttt atattcccac ctacaaattt
     1741 gttttcccat tagtacacta tcaaccaaaa ggagtatccg aatgactgac ctgaacaccc
     1801 tttttgccaa cctcaaacaa cgcaatccca atcaggagcc gttccatcag gcggttgaag
     1861 aagtcttcat gagtctcgat ccgtttttgg caaaaaatcc gaaatacacc cagcaaagcc
     1921 tgctggaacg catcgtcgaa cccgaacgcg tcgtgatgtt ccgcgtaacc tggcaggacg
     1981 ataaagggca agtccaagtc aaccggggct accgcgtgca aatgagttcc gccatcggtc
     2041 cttacaaagg cggcttgcgc ttccatccga ccgtcgattt gggcgtattg aaattcctcg
     2101 cttttgaaca agtgttcaaa aacgccttga ccaccctgcc tatgggcggc ggcaaaggcg
     2161 gttccgactt cgaccccaaa ggcaaatccg atgccgaagt aatgcgcttc tgccaagcct
     2221 ttatgaccga actctaccgc cacatcggcg cggacaccga tgttccggcc ggcgacatcg
     2281 gtgtaggcgg gcgcgaaatc ggctacctgt tcggacaata caaaaaaatc cgcaacgagt
     2341 tttcttccgt cctgaccggc aaaggtttgg aatggggcgg cagcctcatc cgtcccgaag
     2401 cgaccggcta cggctgcgtc tatttcgccc aagcgatgct gcaaacccgc aacgatagtt
     2461 ttgaaggcaa acgcgtcctg atttccggtt ccggcaacgt ggcgcaatac gccgccgaaa
     2521 aagccatcca actgggcgcg aaagtgctga ccgtttccga ctccaacggc ttcgtcctct
     2581 tccccgacag cggtatgacc gaagcgcaac tcgccgcctt gatcgaattg aaagaagtcc
     2641 gccgcgaacg cgttgccacc tacgccaaag agcaaggtct gcaatacttt gaaaaccaaa
     2701 aaccgtgggg cgtcgccgcc gaaatcgccc tgccctgcgc gacccagaac gaattggacg
     2761 aagaagccgc caaaaccctg ttggcaaacg gctgctacgt cgttgccgaa ggtgcgaata
     2821 tgccgtcgac tttgggcgcg gtcgagcaat ttatcaaagc cggcatcctc tacgccccgg
     2881 gaaaagcctc caatgccggc ggcgtagcaa cttcaggttt ggaaatgagc caaaacgcca
     2941 tccgcctgtc ttggactcgt gaagaagtcg accaacgcct gttcggcatc atgcaaagca
     3001 tccacgaatc ctgtctgaaa tacggcaaag tcggcgacac agtaaactac gtcaatggtg
     3061 cgaacattgc cggcttcgtc aaagttgccg atgcgatgct ggcgcaaggc ttctaagcaa
     3121 acgccgccgc tccgcaaaca aaatgccgtc cgaaccgcaa atgctgttca gacggcattt
     3181 cattatccgc ccgttcgaat cgggtgatac
//
LOCUS       DAXHSJ010000186         3170 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000186 DAXHSJ010000000
VERSION     DAXHSJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.186"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            110..2878
                     /gene="tdfH"
                     /locus_tag="ACFM8U_001901"
     CDS             110..2878
                     /gene="tdfH"
                     /locus_tag="ACFM8U_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC6335079.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQDGQLKFNSDSGKWER
                     DLQRQQWKYPWYKKYENPQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYANLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
     gene            2908..3147
                     /locus_tag="ACFM8U_001902"
     CDS             2908..3147
                     /locus_tag="ACFM8U_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335080.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VDFGYFWLFRVIYESSFPQKRESRHSMLRQFIEND"
BASE COUNT          903 a          790 c          765 g          712 t
ORIGIN      
        1 attgaaatct tcagacggta tatcgaattt acacttttta atgtttatgc cgcctaaaaa
       61 aatgctaata tatttcttaa ttgtctgact gtttattgtt gaggaaaata tgagatcttc
      121 tttccggttg aagccgattt gtttttatct tatgggtgtt atgctatatc atcatagtta
      181 tgccgaagat gcagggcgcg cgggcagcga ggcgcagata caggttttgg aagatgtgca
      241 cgtcaaggcg aagcgcgtac cgaaagacaa aaaagtgttt accgatgcgc gtgccgtatc
      301 gacccgtcag gatatattca aatccagcga aaacctcgac aacatcgtac gcagcatacc
      361 cggtgcgttt acacagcaag ataaaagctc gggcattgtg tctttgaata ttcgcggcga
      421 cagcgggttc gggcgggtca atacgatggt ggacggcatc acgcagacct tttattcgac
      481 ttctaccgat gcgggcaggg caggcggttc atctcaattc ggtgcatctg tcgacagcaa
      541 ttttattgcc ggactggatg tcgtcaaagg cagcttcagc ggctcggcag gcatcaacag
      601 ccttgccggt tcggcgaatc tgcggacttt aggcgtggat gacgtcgttc agggcaataa
      661 tacctacggc ctgctgctaa aaggtctgac cggcaccaat tcaaccaaag gtaatgcgat
      721 ggcggcgata ggtgcgcgca aatggctgga aagcggagca tctgtcggtg tgctttacgg
      781 gcacagcagg cgcagcgtgg cgcaaaatta ccgcgtgggc ggcggcgggc agcacatcgg
      841 aaattttggc gcggaatatc tggaacggcg caagcaacga tattttgtac aagacggcca
      901 gttgaaattc aattccgaca gcggaaaatg ggagcgggac ttgcaaagac aacagtggaa
      961 atacccgtgg tataaaaaat acgaaaaccc ccaagaactg caaaaataca tcgaagagca
     1021 tgacaaaagc tggcgggaaa acctggcgcc gcaatacgac atcaccccca tcgatccgtc
     1081 cagcctgaag cagcagtcgg caggtaatct gtttaaattg gaatacgacg gcgtattcaa
     1141 taaatacacg gcgcaatttc gcgatttaaa caccaaaatc ggcagccgca aaatcatcaa
     1201 ccgcaattat caattcaatt acggtttatc tttaaactca tatgccaacc tcaatctgac
     1261 cgcagcctac aattcgggca ggcagaaata tccgaaaggg tcgaagttta caggctgggg
     1321 gcttttaaag gattttgaaa cctacaacaa cgcaaaaatc ctcgacctca acaacaccgc
     1381 caccttccgg ctgccccgtg aaaccgagtt gcaaaccact ttgggcttca attatttcca
     1441 caacgaatac ggcaaaaacc gctttcctga agaattgggg ctgtttttcg acggtccgga
     1501 tcaggacaac gggctttatt cctatttggg gcggtttaag ggcgataaag ggctgctgcc
     1561 ccaaaaatca accattgtcc aaccggccgg cagccaatat ttcaacacgt tctacttcga
     1621 tgccgcgctc aaaaaagaca tttaccgctt aaactacagc accaataccg tcggctaccg
     1681 tttcggcggc gaatatacgg gctattacag ctcggaaggc gaatttaagc gggcattcgg
     1741 agaaaactcg ccgacataca agaaacattg caacccgagc tgcgggcttt atgaacccgt
     1801 attgaaaaaa tacggcaaaa agcgcgccaa caaccattcg gtcagcatta gtgcggactt
     1861 cggcgattat ttcatgccgt tcgccagcta ttcgcgcaca caccgtatgc ccaacatcca
     1921 agaaatgtat ttttcccaaa tcggcgactc cggcgttcac accgccttaa aaccagagcg
     1981 cgcaaacact tggcaatttg gcttcaatac ctataaaaaa ggactgttaa aacaagatga
     2041 tacattagga ttaaaactgg tcggctaccg cagccgcatc gacaactaca tccacaacgt
     2101 ttacgggaaa tggtgggatt tgaacgggga tattccgagc tgggtcagca gcaccgggct
     2161 tgcctacacc atccaacacc gcaatttcaa agacaaagtg cacaaacacg gttttgagtt
     2221 ggagctgaat tacgattatg ggcgtttttt caccaacctt tcttacgcct atcaaaaaag
     2281 cacgcaaccg accaacttca gcgatgcgag cgaatcgccc aacaatgcgt ccaaagaaga
     2341 ccaactcaaa caaggttatg ggttgagcag ggtttccgcc ctgccgcgag attacggacg
     2401 tttggaagtc ggtacgcgct ggttgggcaa caaactgact ttgggcggcg cgatgcgcta
     2461 tttcggcaag agcatccgcg cgacggctga agaacgctat atcgacggca ccaacggggg
     2521 aaataccagc aatgtccggc aactgggcaa gcgttccatc aaacaaaccg aaacccttgc
     2581 ccgccagcct ttgatttttg atttttacgc cgcttacgag ccgaagaaaa accttatttt
     2641 ccgcgccgaa gtcaaaaatc tgttcgacag gcgttatatc gatccgctcg atgcgggcaa
     2701 tgatgcggca acgcagcgtt attacagctc gttcgacccg aaagacaagg acgaagacgt
     2761 aacgtgtaat gctgataaaa cgttgtgcaa cggcaaatac ggcggcacaa gcaaaagcgt
     2821 attgaccaat tttgcacgcg gacgcacctt tttgatgacg atgagctaca agttttaaag
     2881 gcagcccgcg ttttgtggaa aaccgcaatg ccgtctgaaa gcccttcaga cggcatttgt
     2941 ttccccaaac gcatcatcct gccgcaagcc tatgccaatc cgttttatcg catcggcaac
     3001 tcaaagaaaa atccatttca ttcccacgca gggaagccgg ttgatttcgg ttatttttgg
     3061 ttgtttcggg taatttatga gtcgtcattc ccgcaaaagc gggaatctag acattcaatg
     3121 ctaaggcaat ttatcgaaaa tgactgaaac tcaaaaaact ggattcccac
//
LOCUS       DAXHSJ010000187         3147 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000187 DAXHSJ010000000
VERSION     DAXHSJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.187"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(255..1769)
                     /gene="katA"
                     /locus_tag="ACFM8U_001903"
     CDS             complement(255..1769)
                     /gene="katA"
                     /locus_tag="ACFM8U_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC6335081.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            2117..2967
                     /locus_tag="ACFM8U_001904"
                     /pseudo
     CDS             2117..2967
                     /locus_tag="ACFM8U_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
BASE COUNT          696 a          817 c          884 g          750 t
ORIGIN      
        1 ggaaaagttt tcgtgccggt ttgaaccggt ggggatggtg cgggaatgac ggcggaaaag
       61 tgggctgtgg ggcgggaatg acgggatatt ttgcgtttaa taaaaaacgc ccactgaaac
      121 ggcgggggcg ggatggggaa atgccgtctg aaacggtcaa acgacgtttc agacggcatt
      181 tttatgcccg gttatttccg gtagcggacg gcgcgggaca ggatttcttc aatttccatc
      241 cacataatgc ccccttacag caaaccgggc tggccgagtg cggggtcggt cgcgcgggcg
      301 gcttgtgcgt cttcgacagt caatccgagg gcttttgcca cgccttcacc gtatgccgga
      361 tcgcaacggt agcagttgcg gatatggcgg tatttgatga agtcgggcgc gtcgcccatt
      421 gcggcggcgg tgttgccgaa caatgcctgt ttctgcgcgt cgttcatcag gttgaacagg
      481 gcgcgcggtt ggctgaagta gtcgtcatca tcttggcggt agtcccagtg tgccgcgtcg
      541 ccgttgattt tcaaaggcgg ttcagcgaag tcgggctgct gctgccattg tccgaagctg
      601 ttgggttcgt agtgcggcag gctgccgtag ttgccgtcgg ctctgccttg tccgtcgcgc
      661 tggttgctgt gaacagggca acgcggacgg ttgacaggga tttggcggaa gttcacgccc
      721 aagcggtagc gttgcgcgtc ggcgtaattg aacaaacgcg cctgcaacat tttatctggg
      781 ctggcgccga caccgggaac gaggttgctc ggtgcgaagg cggattgttc cacatcggcg
      841 aagaagtttt cgggattgcg gttcaactcg aattcgccca cttcaatcag cggatagtct
      901 tttttcggcc aaactttggt caggtcaaac ggatgataag gtactttttc ggcatcggct
      961 tcgggcatca cttggatgta catcgtccat ttcggaaact cgccgcgttc gatggcttcg
     1021 tataagtcgc gctgatggct ttcgcggtcg tcggcgatga ttttggcggc ttcttcgttg
     1081 gtcaggtttt taatgccttg ttgggtgcgg aaatggaatt tcacccaaaa acgctcgcct
     1141 gcttcgttcc agaagctgta ggtatgcgaa ccgaagccgt gcatatggcg gtagccggcg
     1201 gggatgccgc ggtcgctcat cacgatggta acttggtgca gtgcttcggg cagcagcgtc
     1261 cagaagtccc agttgtttgt ggcagagcgc atattggtgc gcgggtcgcg tttgacggct
     1321 ttgttcaggt cggggaactt acgcgggtcg cgcaggaaga acacgggcgt gttgttgccg
     1381 accacatccc agttgccttc ttcggtatag aacttcaacg caaaaccgcg gatgtcgcgt
     1441 tctgcatcgg ctgcgccgcg ttcgcctgcc acggtggtga aacgggcgaa catctcggtt
     1501 tttttgccga cttcgctgaa gattttggcg cgggtgtatt tggtgatgtc gtgcgttacg
     1561 gtaaacgtac cgaacgcgcc cgaacctttg gcgtgcatac ggcgttcggg gatgacttcg
     1621 cgcacgaagt cggcgagttt ttcattcagc cacaaatcct gcgccagcag agggccgcga
     1681 ggaccggcgg tcaggctgtt ttgattgtcg gcaacaggcg cgccgttgtt catggtcaga
     1741 tgggttacag ggcatttgga ggtagtcatc gctcttgttc cttttctcag gttggtcaaa
     1801 tgggggtaaa cggcttacag tacgatttgg cggaaagcgt attcgtaacc ggtttcttga
     1861 ttgcaataaa tttcttgaat cgacatttta tttccctttt gtaaaaacta tggatgcgac
     1921 tatacgccaa gattttcgct attaaaacta tgaaatcgat ttaatattat tataagcaat
     1981 cggttcttga ttttcgtttg ttttttgtta tcgaacggaa tccgaacccg ctcattaaaa
     2041 ccatttataa tgcaatgacg ctttgcggca ttttttgcgc cgacaggctg aaaataacaa
     2101 ttttccccac attatcatga ccttactcgg atataagctc aaacagaccc agcagctcaa
     2161 ccagcggctg caacaatctt tgcgcgtatt gcagatgtcg ggtatcgaac ttgaacgcga
     2221 ggtcgaaaac tggctgtcgg acaatcccct gctcgaacgc aaagacacgg atgaattttc
     2281 cgatgccggg tttcccatta caccgcgccc gcgcgtcaaa tcggcggaga cgaaggcgaa
     2341 gatatgctgt ccaacatcgt cggcgaagaa gatttcaagc aatacctgca cgcgcaagtg
     2401 tgcgaacacc cccttcccga ccaagaagcc gcctgtgtcc acatccttat cgatttcctt
     2461 gacgagcagg gttatctgac cgacagcatc gaagacatcc tcgaccatac gcccttagag
     2521 tggatgttgg atgaagcaat gctgcaacac gcgctgaccg cattgaaaaa attcgacccg
     2581 gcaggcgtgg ccgccgccga tttgaacgaa tcgctgatac tgcagataga aagattgggc
     2641 gaatgtgctg ccaaacccgc cgccctgcat atcgtccgaa acgccctcga cagcattgac
     2701 ggcaaccgca gccaaaccct cgcacgaata aaaaaacacc tgccccaaac cgacagcggc
     2761 acactcgaag ccgcactcga cctcattgct tcgctcaatc cctttcccgc cgccggtttt
     2821 gcctcgtcca cacccacgcc gtattctgac gaggcgctcg ccaacctgct ggctttccgc
     2881 ggcatggagg tttctcgccg caccattgcc aaatacagag aatcctttga gattccggca
     2941 gcacacaaac gcaaaaccgc agaataattg ccgaataatc ttataaagac aacaaaccaa
     3001 aagccggcat ttctgcgaaa gcgggaatgc cgaatccgtc cgcgcggaaa cctgcatccc
     3061 gtcattcccg cgaaagaggg aatctagaaa cgcaaagttg caagaattta tcggaaatga
     3121 ccgaaactca acgaacctgg attcccg
//
LOCUS       DAXHSJ010000188         3062 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000188 DAXHSJ010000000
VERSION     DAXHSJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.188"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..292)
                     /locus_tag="ACFM8U_001905"
     CDS             complement(<1..292)
                     /locus_tag="ACFM8U_001905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335082.1"
                     /translation="MIKDPSFPRRRESGLFGFSCFWVSGNFQIVIPAQAGIQTIGQRQ
                     YSKIIRKFEVLDSRFHGNDERLWESRPSGSGNIQRLSESLEVLDSRFHGNDGM"
     gene            complement(295..1287)
                     /gene="pdxA"
                     /locus_tag="ACFM8U_001906"
     CDS             complement(295..1287)
                     /gene="pdxA"
                     /locus_tag="ACFM8U_001906"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC6335083.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAPGEGELEVLHIPAAEAVESGQLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1446..2873
                     /locus_tag="ACFM8U_001907"
     CDS             1446..2873
                     /locus_tag="ACFM8U_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6335084.1"
                     /translation="MNENFTEWLHGWINAINDQMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRVSKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
BASE COUNT          633 a          838 c          852 g          739 t
ORIGIN      
        1 acattccgtc attcccgtga aaacgggaat ctagaacctc taaactttca gataatcttt
       61 gaatattgcc gctgcccgaa ggtctggatt cccacaacct ttcgtcattc ccgtgaaaac
      121 gggaatctag aacctcaaac tttcggataa tctttgaata ttgccgctgt ccaatggtct
      181 ggattcccgc ctgcgcggga atgacgattt ggaaattacc cgaaacccaa aaacaactga
      241 aaccgaacag accggattcc cgcctgcgcg gaaatgacgg gtcttttatc atctttaaag
      301 gctgccgcgc gccatctcga cggcggtctc cacggcagtt atcaggctgc cggaatccgc
      361 cctgccggtt gccgccaaat caagcgcggt gccgtgatcg acggaggtgc ggataaaggg
      421 cagacccagc gtgatgttca cgccctgtcc gaagctgtgg tatttcaaca cgggcagccc
      481 ttggtcgtgg tacatcgcca atacggcatc cgcaccttcg agcataaacg gttggaacaa
      541 ggtatccgcc ggatacgggc cggcgaggtt tatcccttcg cggcgcaggt tttcaagcgc
      601 ggggataatg gtgtcggttt cttcgtgtcc gaggtgtccg ccctcgccgg cgtgggggtt
      661 gagtccggcg actaggattt tgggattttt gatgccgaat ttgtgtttta agtcgtgatg
      721 cagaatgcgt gcgacgcttt caatcagcgg ttgcgtgatg gcggcggcaa cgtctttcag
      781 cggcaggtgg gtcgttacga gggcgacgcg caggcctttg ccggcaagca tcatcacaac
      841 ctgccccgtg ccgctttttt ccgccagata ttcggtgtgt ccgctgaaaa aacctgtgct
      901 tgcgcgcgcg tcgttgatga tgcctttgtg cagcggcgcg gtaacgatgc cgtcgaaaat
      961 gccgtctgaa atgcctgcga gcgcggtgtc caaaagttgc agcacatagg cggcgttggc
     1021 gggattaagc tgcccggact caaccgcttc ggcggcaggg atgtgcagca cttccagctc
     1081 gccttctccg ggtgcgccgc ctgattccgg atcgaagtcg cgcaggacga cgcttttgcc
     1141 caaggcttcg gcgcgcgcgc gcaacaggtg tttgtcgccc aataccgcgc agcggcaggg
     1201 caggcgtgca aacgccaagt ccaaacaaat atcggggccg atgccggcag gctcgccgga
     1261 agtaacggca aaaacaggct gtttcatcgt gtttgctcca aacaaaatgc gattctaacg
     1321 ccgcagccgc gcggcgatgt aaatttttct gattttgttg acaatctgct agaatagaag
     1381 tttacaaaat ttaaaccctg cttacatacc gccatatgtg cgggtttcaa ctttaaggaa
     1441 gcgatatgaa cgagaacttt accgaatggc tgcacggctg gataaacgcc atcaacgacc
     1501 aaatgtggtc atacttggtt tatatgcttt tgggtacggg gcttttcttc accgtaacca
     1561 cgggctttgt ccaattccgc ctgttcgggc gcagcatcaa agaaatgctc ggcggccgca
     1621 aacaggggga cgaccctcac ggcatcacgc cgtttcaggc atttgtaacc ggccttgcca
     1681 gccgcgtggg cgtgggcaat atcgcgggcg tggcaatcgc catcaaagtc ggcgggccgg
     1741 gcgcggtgtt ctggatgtgg gtaaccgcct taatcggtat gagttcggcg tttgtcgaat
     1801 cttcgctggc gcagctcttt aaagtccgcg actacgacaa ccaccacttc cgtggcggcc
     1861 ctgcctacta catcactcaa gggctggggc agaaatggct gggcgtgttg ttcgccctga
     1921 gcctgatttt ctgtttcggc tttgtgtttg aagccgtaca gaccaatacc atcgccgata
     1981 ccgtcaaagc ggcgtggggc tgggagcctc attatgtcgg cgttgccctg gtgattttaa
     2041 ccgcgccgat tatcttcggc ggcatcaggc gcgtatctaa agcggcggaa atcgtcgtcc
     2101 ccctgatggc ggttttgtac ctctttatcg cgcttttcat cattttgacc aatattccga
     2161 tgattccgga cgtgttcggt cagatttttt cgggcgcgtt caaattcgac gcggcagcag
     2221 gcggcttact cggcggtctg atttcgcaaa cgatgatgat gggcatcaaa cgcggcctgt
     2281 attccaacga ggcgggtatg ggttccgcgc cgaacgccgc cgccgccgcc gaagtgaaac
     2341 accctgtttc acaaggtatg attcaaatgc tgggcgtgtt tgtcgatacc atcatcgttt
     2401 gttcttgcac cgccttcatc atcttgattt accaacagcc ttacggcgat ttgagcggtg
     2461 cggcgctgac gcaggcggcg attgtcagcc aagtggggca atggggcgcg ggcttcctcg
     2521 ccgtcatcct gtttatgttt gccttttcca ccgttatcgg caactatgcc tatgccgagt
     2581 ccaacgtcca attcatcaaa agccattggc tgattaccgc cgtcttccgt atgctggttt
     2641 tggcgtgggt ctatttcggc gcggttgcca atgtgccttt ggtctgggat atggcggata
     2701 tggcgatggg cattatggcg tggatcaacc ttgtcgccat cctgctgctc tcgccactgg
     2761 cgtttatgct gctgcgcgat tacaccgcca agctgaaaat gggcaaagac cccgagttca
     2821 aactttccga acatccgggc ctgaaacgcc gtatcaaatc cgacgtttgg taaatcccgc
     2881 ccttaccgga gccgcttccc cgcgaagcgg cttttccctt tccgcacact gtaaaaacag
     2941 ggcgaacaag cgtacaatcc caacccttta cttttgaatc catttcgttt ttcagacggc
     3001 atattgaata tagtggttta acaaaaatca ggacaaggcg acgaagccgc agacagtaca
     3061 ag
//
LOCUS       DAXHSJ010000189         2968 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000189 DAXHSJ010000000
VERSION     DAXHSJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.189"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            123..1667
                     /gene="purF"
                     /locus_tag="ACFM8U_001908"
     CDS             123..1667
                     /gene="purF"
                     /locus_tag="ACFM8U_001908"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC6335085.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNADSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTIITRQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLNKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            1767..2258
                     /gene="greB"
                     /locus_tag="ACFM8U_001909"
     CDS             1767..2258
                     /gene="greB"
                     /locus_tag="ACFM8U_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC6335086.1"
                     /translation="MSTETKNYITPAGWQALKDELYRLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            2317..2943
                     /locus_tag="ACFM8U_001910"
     CDS             2317..2943
                     /locus_tag="ACFM8U_001910"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC6335087.1"
                     /translation="MRKIRTKICGITTLEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT          711 a          911 c          777 g          569 t
ORIGIN      
        1 cttattctcc ataaacccac ctatctgtcc ttcgctcaaa tattgtcaac aattcaggca
       61 aaaaaccacc gtttacttta taattccgtt ttacccttaa acctcgaatc ggagaagacg
      121 atatgtgcgg cgtattaggt ttggtcagtc atgaacccgt gaaccagctt ctgtacgacg
      181 gtttgcagat gttgcagcac agggggcagg atgcggcggg cattgcaacg gcggaaggcg
      241 gtaccttcca tatgcacaaa ggcaaaggca tggtgcgcga agtgttccgc acacgcaata
      301 tgcgcgattt ggtcggcaac gccggcatcg cccacgtccg ttatcctact gcgggcaacg
      361 ccgacagcag cgcggaggcg cagcctttct acgtcagctc gccgttcggc atcgttttgg
      421 cgcacaacgg caatctcacc aacacggccg aactgtatga aaacgtgtgc aacaaacacc
      481 tgcgccacgt caacaccagc tccgattccg aagtcttgct caacgtattc gcgcacgaat
      541 tacgccgcga agtctctaaa aacgccgacc cgcaccggct caatgccgac aatattttca
      601 acgccgttgc cgaagtccac cgcctggtgc gcggcgcata cggcgtggtt gccatgattg
      661 cgggctacgg tatgctcgcc ttccgcgacc cttacggcat ccgcccgctg gtattgggtt
      721 cgcaaaccga cagcgaaggc agaaaatcct atgccgtcgc ctccgaatcc gtcgccttca
      781 acgcgcttac ctacgatttg gaacgcgata tccggccggg cgaagcggta tttgtcggct
      841 ttgacggcac aatcatcacc cgacaatgca gcgaccgcgc caaactcagc ccctgccttt
      901 ttgaatatgt ttattttgcc cgccccgact ctgtgattga cggcgtatcc gtttaccagt
      961 cgcgcttgga tatgggcgtg tccttggcgg aaaaaatcaa acgcgagctg cccgtggacg
     1021 gcatcgacgt tgtaatgccc atccccgaca ccagccgccc cagcgcgatg gagcttgccg
     1081 tccacctcaa caaaccctac cgcgagggct tgattaaaaa ccgctatatc ggccgcacct
     1141 ttattatgcc cggccaggcg acgcgcaaaa aatccgtgcg ccaaaaactc agcccgatgg
     1201 aaaccgaatt tgcaggcaaa agcgtgttgc tggtggacga ctccatcgtg cgcgggacga
     1261 ccagccgcga aatcgtcgaa atggtacgcg cggctggcgc gcgcaaagtc tatatcgcct
     1321 ccgccgcgcc cgaagtgcgc tatcccaacg tatacggcat cgatatgccc acacgcgaag
     1381 agttgattgc caatgggcgc agcgcggcgg aaattgccgc cgaaatcggc gcggacggca
     1441 tcgtgtttca agacttgggc gatttggaag ccgtcgtcaa agcactcaat ccgaaaatcg
     1501 aatctttcga ttcgtcctgt ttcaacggca tttatcaaac cggcgacatc gatgatgcct
     1561 acctcgaccg cctgtctgcc gagaaatccg gctgcggcgg cctgaaaatc cacccgagca
     1621 ggatggaaca cagcatcagc atcagcgatg cgggcgacga agaataaggc ttcccaaacc
     1681 gcgatgccgt ctgaaatccg aacgccgggt tttcagacgg catttcccat atcggcatca
     1741 catttcccat ttcatccgca cgcaatatga gtaccgaaac caaaaactac attacgcccg
     1801 ccggatggca ggcattgaaa gacgagcttt accggctggt caacaaagaa cgccccgaaa
     1861 tcgtccaaat cgtcaactgg gcggcaggca acggcgaccg cagcgaaaac ggcgactatc
     1921 tttacggcaa acgccgtatg cgcgaaatcg accgccgcat ccgtttcctg accaaacgtt
     1981 tggagtctgc cgtcgtcgtc gatcccgaat tgcgcgaagc gaccgaccaa gtgtttttcg
     2041 gcgcgacggt cgggctgttg cgcggcgacg ggcgcgagca aaccgtcaaa attgtcggca
     2101 tcgatgaaat cgataccgcg caaaacaaaa tttcttggat ttcgccgttg gcgcgttgcc
     2161 tgattaaagc gcgcgaaggc gacgaagtgg ttttgaacac gccggaaggg cgtgaggaaa
     2221 ttgaaatcct ttctgtcgaa tacatcaaaa tcgactgatc gggaatgccg tctgaaacgg
     2281 cacaaacgca tcgtttccca aataaaggaa taccgaatga ggaaaatccg caccaaaatc
     2341 tgcggcatca ccacactgga agacgcagcg gccgccgcag cggcaggtgc ggatgccgtc
     2401 gggctggtct ttttccaagg cagcagccgg gccgtcgata ttgcccgcgc caaaaaaatc
     2461 accgccgcac tgccgccgtt tgtcagcgtt gtcgcccttt tcgtcaacga aagcgcgcaa
     2521 aacatccgcc gcatccttgc cgaagtgccg atacacatca tccaattcca cggcgacgaa
     2581 gacgacgcat tctgccgcca gttcgaccgc ccctatatca aggccattcg tgttcagacg
     2641 gcatcagaca tccgaaacgc cgccgaccgc ttccccgacg ctcaggcact gctgttcgat
     2701 gcctaccacc cttcggaata cggcggcacc ggacaccgct tcgactggac gctgctggcg
     2761 gaatattcgg gcaaaccgtg ggtgcttgcc ggcgggctga cccctgaaaa cgtcggcgaa
     2821 gccgtccgca tcaccggagc ggaagcggtc gacgtatccg gcggcgtgga agcgtctaaa
     2881 ggcaaaaaag acccagccaa agtcgccgcc tttatcgcaa ccgccaaccg cctatcccgt
     2941 taaagcaaca aaaattgccg ctggaatg
//
LOCUS       DAXHSJ010000190         2955 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000190 DAXHSJ010000000
VERSION     DAXHSJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.190"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(124..459)
                     /locus_tag="ACFM8U_001911"
     CDS             complement(124..459)
                     /locus_tag="ACFM8U_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335088.1"
                     /translation="MMADKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
     gene            complement(471..830)
                     /locus_tag="ACFM8U_001912"
     CDS             complement(471..830)
                     /locus_tag="ACFM8U_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335089.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKNGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDS
                     YKVNWKINNVNLIQTTN"
     gene            complement(1049..1504)
                     /locus_tag="ACFM8U_001913"
     CDS             complement(1049..1504)
                     /locus_tag="ACFM8U_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335090.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     RTEYYESPDSNLLIAVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIITGGKDNYSTEPYYDE"
     gene            complement(1485..2114)
                     /locus_tag="ACFM8U_001914"
     CDS             complement(1485..2114)
                     /locus_tag="ACFM8U_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC6335091.1"
                     /translation="MVKTADGYKAIAHIQAGDRVLSKDEASGVTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQIS
                     AESRIAELKNKIIPGMHKNERLKIERTIRNIAKNANRKAKGEEHGRRGR"
     gene            complement(2259..2489)
                     /locus_tag="ACFM8U_001915"
     CDS             complement(2259..2489)
                     /locus_tag="ACFM8U_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335092.1"
                     /translation="MEKITQQYAYSELLRLFNQNASDEKIANLAFDFLYAWSKDNSPE
                     SRNIIYDLALIGEPGMELTRNDIKELIDSLVE"
     gene            complement(2477..>2955)
                     /locus_tag="ACFM8U_001916"
     CDS             complement(2477..>2955)
                     /locus_tag="ACFM8U_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335093.1"
                     /translation="ANILPFAKAEKLAKLGKAAKPGRAAKLGKAKVSEDFAVATQAKY
                     KQYGDAHSANAQSALAKKLSGLEDAQNSASRTKTLPDGRIRYYEAERFARTEGRTRGN
                     SFVTEYNPKTGSTRQWTENYDHSGNVTMVHPKSINGQSVTSQHYPPTKQELDSWKK"
BASE COUNT          790 a          626 c          537 g         1002 t
ORIGIN      
        1 agccaacaac ttcggtaaaa tcaagccggc aagcccctgc atcctgtgct aatcggagaa
       61 aatgccgtct gaaagccgaa taccgcttca gacggcattt gccagatttg accttgccgg
      121 tgcttacgga cgctttttaa atataaagaa ggaaatatcg tctaacgcat cttcggcaaa
      181 accttgaaaa tccggattgc cttgttttga gcgcaataaa gcaatcactt tatcgcaatc
      241 gccgatattt tcatgcatcc tggcgagatg tccgaaacag gttaatgaca atctggcaat
      301 atccaagtct ttatgcttcg acaactccgt taatttctca gaaatccaag ggatttcatc
      361 ctcctctgaa ctgaaggtca taccgagcat accgtcaata acactctgtt tattctcaga
      421 ataaatcatc ttttctactt gttccttatc agccatcatt ttttgtccat ttaattagtc
      481 gtttgtatta aattgacatt atttattttc caatttactt tataactatc ttcataataa
      541 tctaattcaa aaaaacctga tatttcaata tccaattcca ttattgtttt aatgcatttt
      601 tcaaaataaa taatgaaata agatttcacg catgcgccaa aaaaaatata gctgctccaa
      661 ttaaaactat ttgtcgggaa aacccaccca tttttatata tttttgcaga ttctttctct
      721 tcgatattga agggacaatt attccaaaaa ttattaatgt tttcttggat gatttttata
      781 tcatcgtcag agcattcaat ccatcctttt atggaaacat atgatgccat ttaatctcct
      841 aaacctgttt taacaatgcc gccttttgat tcaatatatg acttaacttg tgaatgaaca
      901 ccgtgcctgt tccgttcagc aagcacaggt tggattaaga ttcgacaatc tgtttggatt
      961 gacagaaagg tcgtctgaaa aatcgattac ggtttcagac gaccttttgt tgttgagttc
     1021 tcaacccaac ctatgcctgc cattaatgtt attcatcgta gtaaggttct gttgaataat
     1081 tgtctttgcc cccggtaatg atagtaacaa ttttcccttt tgcttcccaa gcttgtactc
     1141 ctatttcatc aaactcatag acatatgtcg gataagattc atttgataaa taatatttat
     1201 caacaccgta tgatttaggg tgatggaaaa gctgtttaaa atcttcaaaa ttcagaccta
     1261 ttatattaac gcccataaaa tatagctcct gataacaaaa tatcgaaata attttgtttt
     1321 tcttgacggc aattagtaaa tttgagtcgg gagattcata atattctgtt ctaaactcat
     1381 caggaggttc atacataaaa gtttccagta tgtttttgta ctgtgttata tccgcaccaa
     1441 aacggaatat tcctacagaa gtaaaaggta aaaattcggg agttttaacg accgcgtcga
     1501 ccatgctctt ctccttttgc ttttcgattg gcattttttg caatatttct gattgttcgc
     1561 tcaatcttta atcgttcatt tttgtgcatt ccgggaataa ttttattttt taattcagca
     1621 attcgtgatt ctgctgatat ttgacttcga ccgccatctc catgtttttc attcttggag
     1681 cttcctgttc ttttaggcgg acaagaatta tgaacccaaa ccccttccgt ctccgcctga
     1741 ttgcccttga cgaagtaagt atgccagtcg gcaacggtca gattgtaggc tttgagcggc
     1801 tgctgtttga gggtaatgtt ttgaactgtc tgttttgcac cgctttcgga aagcagggtg
     1861 tcgccttttt tcagacgacc tgcctgtatc cactttcctt gactgtaaaa cggatggatg
     1921 cggttggaaa tcagggtttg gctgttgccg atgccgtctg aaatttcaat gtaaacggtt
     1981 tcttgatacg gattgccgta tcgggcggta acaggtttgt atcccgttac cccgcttgcc
     2041 tcgtccttgg aaaggacgcg gtctccggct tgaatatggg caatagcttt gtagccgtct
     2101 gccgttttga ccaaggtgct gtcgtggaag gagcaggtgt aggatttttg aagaacttag
     2161 ttctcaatat cctgtttcaa atgaggggaa ggtcacctga aaatcaaaaa cggtttcaga
     2221 caaccttttg attgtgggat tttcagccca acctatgctt attcaactaa actgtcaatc
     2281 aattctttta tgtcattacg ggtcagttcc atacccggtt caccgataag tgctaaatca
     2341 tatattatat tacgcgattc tggagaatta tcttttgacc aagcatacag aaaatcaaaa
     2401 gcaagattcg caattttttc atccgaagcg ttttgattga acaatctgag cagttcagaa
     2461 tatgcgtatt gttgagttat tttttccatg aatctaattc ctgttttgtt ggtggataat
     2521 gctgagaggt tacactttgt ccgttaattg atttaggatg aaccatagtt acgttcccgc
     2581 tatggtcata attttctgtc cattgtcggg tactacctgt tttaggatta tattctgtaa
     2641 caaagctatt acccctcgtt cttccctcag ttctagcaaa tcgctcagct tcataatacc
     2701 tgattctccc atcaggtaaa gttttcgtcc tagacgcact attttgagca tcttccaaac
     2761 ctgacaattt cttagctaat gccgattggg cattggcaga atgcgcatcc ccatattgct
     2821 tgtatttggc ttgtgtggca acagcaaaat cctcgctaac ctttgccttc cctagttttg
     2881 ccgccctccc cggttttgcc gccttcccca gtttcgccaa cttctcagct ttggcaaacg
     2941 gcaggatgtt ggcca
//
LOCUS       DAXHSJ010000191         2945 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000191 DAXHSJ010000000
VERSION     DAXHSJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.191"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(107..271)
                     /locus_tag="ACFM8U_001917"
     CDS             complement(107..271)
                     /locus_tag="ACFM8U_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335094.1"
                     /translation="MRFVVLASLQAAFSGRSCNESVIGFDVSLRLTALSLTLSHRERG
                     RVAVASLFAA"
     gene            302..625
                     /locus_tag="ACFM8U_001918"
     CDS             302..625
                     /locus_tag="ACFM8U_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6335095.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            739..2916
                     /gene="tdfF"
                     /locus_tag="ACFM8U_001919"
     CDS             739..2916
                     /gene="tdfF"
                     /locus_tag="ACFM8U_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
                     /protein_id="HFC6335096.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKALDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTATTDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDLSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQGETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          712 a         1042 c          746 g          445 t
ORIGIN      
        1 gcttccttcg aaagctccgt tttcagacga ccttttggaa caccacaggc acacgcatcg
       61 aacggctgaa tcaaagattc agaccgatgg cagtccgcac ccgagtttat gcggcaaaca
      121 gcgaggctac ggcaacccgc cccctctccc tgtgggagag ggttagggag agggcggtaa
      181 gccgcaggct tacatcaaag ccgataacgc tttcgttaca actccgccca ctgaaagcag
      241 cctgcaacga agccaaaacg acaaaccgca tcgtaaacca cccaacccat aggagaaccc
      301 catgcaaaac gaaaccatca acctgaaaca gcaccttgcc gccatcaaag aatactggca
      361 gcccgaaatc atcaaccgcc acgggttcca attccgtttg gtcaaacttt tgggcgatta
      421 cggctggcat acgcacggat acagcgacaa agtgctgttt gccgtggagg gcgacatggc
      481 ggtggacttc gccgacggcg gcagcatgac gatacgcgag ggcgagatgg cggtcgtgcc
      541 gaagtcggtg tcgcaccgcc cgcgttcgga aaacggctgc tcggtggtgc tgattgagtt
      601 gtccgacccg tccgaggccg tctgaaaacg aagtttccga aggaagctga gtttctgcga
      661 agctaaaagc agcctgcacc ttcaatcaat atgccgaaaa tacaacccca ccgcacacca
      721 acacacaaag gaaatcccat gacacgcttc aaatattccc tgctgtttgc cgccctgttg
      781 cccgtgtacg cgcaggccga tgtttctgtt tcagacgacc ccaaaccgca ggaaagcact
      841 gaattgccga ccatcaccgt taccgccgac cgcaccgcga gttccaacga cggctacact
      901 gtttccggca cgcacacccc gctcgggctg cccatgaccc tgcgcgaaat cccgcagagc
      961 gtcagcgtca tcacatcgca acaaatgcgc gaccaaaaca tcaaagcgct cgaccgcgcc
     1021 ctgttgcagg cgaccggcac cagccgccag atttacggct ccgaccgcgc gggctacaac
     1081 tacctgttcg cgcgcggcag ccgcatcgcc aactaccaaa tcaacggcat ccccgttgcc
     1141 gacgcgctgg ccgatacggg caatgccaac accgccgcct atgagcgcgt agaagtcgtg
     1201 cgcggcgtgg cggggctgct ggacggcacg ggcgagcctt ccgccaccgt caatctggtg
     1261 cgcaaacgcc cgacccgcaa gccattgttt gaagtccgcg ccgaagcggg caaccgcaaa
     1321 catttcgggc tgggcgcgga cgtatcgggc agcctgaaca ccgaaggcac gctgcgcggc
     1381 cgcctggttt ccaccttcgg acgcggcgac tcgtggcggc agcgcgaacg cagccgcgat
     1441 gccgaactct acggcatttt ggaatacgac atcgcaccgc aaacccgcgt ccacgcaggc
     1501 atggactacc agcaggcgaa agaaaccgcc gacgcgccgc tcagctacgc cgtgtacgac
     1561 agccaaggtt atgccaccgc cttcggcccg aaagacaacc ccgccacaaa ttgggcgaac
     1621 agccgccacc gtgcgctcaa cctgttcgcc ggcatcgaac accgcttcaa ccaagactgg
     1681 aaactcaaag ccgaatacga ctacacccgc agccgcttcc gccagcccta cggcgtagca
     1741 ggcgtgcttt ccatcgacca caacaccgcc accaccgacc tgattcccgg ttattggcac
     1801 gccgacccgc gcacccacag cgccagcgtg tcattgatcg gcaaataccg cctgttcggc
     1861 cgcgaacacg atttaatcgc gggtatcaac ggttacaaat acgccagcaa caaatacggc
     1921 gaacgcagca tcatccccaa cgccattccc aacgcctacg aattttcccg cacgggtgcc
     1981 tacccgcagc ctgcatcgtt tgcccaaacc atcccgcaat acggcaccag gcggcaaatc
     2041 ggcggctatc tcgccacccg tttccgcgcc gccgacaacc tttcgctgat actcggcggc
     2101 agatacagcc gttaccgcac cggcagctac gacagccgca cacaaggcat gacctatgtg
     2161 tccgccaacc gtttcacccc ctacacaggc atcgtgttcg acctgaccgg caacctgtct
     2221 ctttacggct cgtacagcag cctgttcgtc ccgcaatcgc aaaaagacga acacggcagc
     2281 tacctgaaac ccgtaaccgg caacaatctg gaagccggca tcaaaggcga atggcttgaa
     2341 ggccgtctga acgcatccgc cgccgtgtac cgcgcccgta aaaacaacct cgccaccgca
     2401 gcaggacgcg acctgagcgg caacacctac taccgcgccg ccaaccaagc caaaacccac
     2461 ggctgggaaa tcgaagtcgg cggccgcatc acgcccgaat ggcagataca ggcaggttac
     2521 agccaaagca aaacccgcga ccaagacggc agccgcctga accccgacag cgtacccgaa
     2581 cgcagcttca aactcttcac tgcctaccac tttgcccccg aagcccccag cggctggacc
     2641 atcggcgcag gcgtgcgctg gcagggcgaa acccacaccg accctgccac gctccgcatc
     2701 cccaaccccg ccgccaaagc ccgcgccgcc gacaacagcc gccaaaaagc ctacgccgtc
     2761 gccgacatca tggcgcgtta ccgcttcaat ccgcgcgccg aactgtcgct gaacgtggac
     2821 aatctgttca acaaacacta ccgcacccag cccgaccgcc acagctacgg cgcactgcgg
     2881 acagtgaacg cggcgtttac ctatcggttt aaataagatc gtctgaaaac ggagtttctg
     2941 cgaag
//
LOCUS       DAXHSJ010000192         2883 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000192 DAXHSJ010000000
VERSION     DAXHSJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.192"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(36..512)
                     /gene="greA"
                     /locus_tag="ACFM8U_001920"
     CDS             complement(36..512)
                     /gene="greA"
                     /locus_tag="ACFM8U_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC6335097.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            904..2205
                     /gene="aroA"
                     /locus_tag="ACFM8U_001921"
     CDS             904..2205
                     /gene="aroA"
                     /locus_tag="ACFM8U_001921"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC6335098.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGKEHYPPLHIGKRQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADARYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPKTLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETTE"
     gene            complement(2248..2781)
                     /locus_tag="ACFM8U_001922"
     CDS             complement(2248..2781)
                     /locus_tag="ACFM8U_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6335099.1"
                     /translation="MFPPDKTLFLCLSALLLTSCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELILHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          611 a          782 c          789 g          701 t
ORIGIN      
        1 cctgcgtgtg tcgggtgtat cgaaatcaag ccgaatcaaa tatatcggac ttcgataatg
       61 tcgtattcgc gcacgccgcc cggggcttgg acttccgccg tatccccctc ttccttgccg
      121 attaaggcgc gggcgatggg tgagccgaca tagattttgc cctgtttgat gtcggcttcg
      181 tcttcgccga caatttgata ggtaacgtgt tcttccgttt ccaaatcttc cagcgtaacc
      241 gtcgtaccga acacgatttt gccttcggcg tggatttcgg tcggattgat gatgtgggca
      301 acggaaagtt tgtgttccaa ctcggaaatg cggccctcga taaaaccttg gcgttctttg
      361 gcggcttcgt attcggcgtt ttcggacaaa tcgccgtgcg atctggcttc agcaatcgct
      421 tcgatcactt cggggcgtgc cacgcttttg agctgctgca attcctgttt cagcaattcc
      481 gcaccgcgta cggtcagggg gattttttgc atcggtcttt tttctccata ttccggcaca
      541 ccgttttgcg gcagcaagca taccgcgtac cgtcttgttt tgtgcgtccg gatattaaaa
      601 taaaaataca agccgcccgg aaaatcggcg gcttgtctgt cgttgaacag cggctattct
      661 accaaattct atgaaattgg caatcgtgcc gcgccgccgg caaacgcgcc atgtccgcaa
      721 caaaagctga aaatatgccg acaaagaaat tttagaaaca aaaaatttaa aaataatcaa
      781 ttttcggcat aaaaaaccac atttacggac tttaaaaccg aaaatgccaa acctgagatt
      841 tttcatacgg catttgcacc agtataatgc aggctgtttt tatctttata tttacgtttt
      901 gccatgaccg aatccgtccg cctccccgtc gcccgcctca aaccttccac cgtcgccctg
      961 cccggctcca aaagcatcag caaccgcacc ctgctgctcg ccgccttgtc cgacaatgct
     1021 tgcgaaatcc attccctgct caaatccgac gatactgacc gtatgctcga agcactcgat
     1081 aaactcggcg ttgaaatcga atatcttgcc gaagaccgtc tgaaagtgca cggcacaggc
     1141 ggacgcttcc ccaaccgcac tgccgatttg tttttaggca acgcgggaac ggcgttccgt
     1201 ccgctgactg ccgcgcttgc cgttttgggc ggcgattatc atctgcacgg cgtgcctcgt
     1261 atgcacgaac gtcctatcgg cgatttggtc gatgcgctgc ggattgccgg cgccgatgtc
     1321 gaatatctcg gcaaggaaca ctatccgccg cttcatatcg gcaaacgtca agacaacggc
     1381 gaacgcgtga ttccgattaa aggcaatgtg tccagccagt ttctgaccgc ccttttaatg
     1441 gcgttgccgc tgaccgggca ggcgtttgaa atccgtatgg tcggcgagtt aatttccaag
     1501 ccctatatcg acattacttt aaaactgatg gcgcaattcg gcgtacaggt tatcaatgaa
     1561 ggctaccgcg tcttcaaaat tcccgccgat gcgcgctacc acgcgcccga acacttgcac
     1621 gtcgaaggcg atgcctccag cgcgtcctac ttcctcgcag ccggtttgat tgccgccacg
     1681 cccgtccgcg ttaccggtat cggcgcaaac agcatacagg gcgatgtcgc ctttgcccgc
     1741 gagctggaaa aaatcggtgc ggacgtggtt tggggcgaaa acttcgtcga agtctcgcgc
     1801 ccgaaagaac gtgccgtcca agcctttgat ttggatgcaa accatatccc cgatgccgcc
     1861 atgaccctcg ccatcgtcgc gcttgctaca gggcaaacct gcacgcttcg caacatcggt
     1921 tcgtggcgcg tcaaagagac cgaccgcatt gccgcaatgg cgaacgagtt gcgcaaactc
     1981 ggggcaaaag tcgtcgaaga agccgaagca attcacatca ccccgcccaa aacgctgaca
     2041 cccgacgccg tcatcgacac gtacgacgac caccgcatgg cgatgtgttt ctcgctggtt
     2101 tcgctgttgg gcgtacccgt cgtcatcaac gatccgaaat gcacccataa aaccttcccg
     2161 acttatttcg acgtgttctc ttcactgacc gaaacaacgg aataaggcgg cattttgccg
     2221 cgattccggc gcggcgcggc gggcggctca ttctgtaaaa aaagtatgtg cgccgaggta
     2281 gtttttggcg taaaacggtg tggagagttt ttcggttttg atggttttgc cgctgctggg
     2341 ggcatggatg aattcgccgt tgccgatgta gagtccgacg tgtgagtagc ggtgtgcgcc
     2401 gccggtgttg aagaatacga ggtcgccggc cttgaggcgg ctgtcgggaa ttttgcggct
     2461 tgccgccgcc atgtcacggg cggtgcgcgg cagcttgacg ttgagggcgt ttttgtaaac
     2521 gaattggatc atgccgctgc aatcgaagcc ggttgcggtg ctgctgccgc cccatttgta
     2581 gggcgtgccg atgagtccga ggctgtggag tatgagttcc tgcgagcctt gtgtgcggtc
     2641 gatgtggctg atgcggacgg cttggatttg ccggactgtc tgtttgggtt tcggttggcg
     2701 gtgtttgccg gaggttgtgc cgcatgaggt gaggagcagt gcgctgagac agaggaaaag
     2761 ggttttgtcg gggggaaaca tggtttttcc tttgcgggtt cggatatgcc gtctgaaggt
     2821 gtttcagacg gtatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
     2881 acg
//
LOCUS       DAXHSJ010000193         2789 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000193 DAXHSJ010000000
VERSION     DAXHSJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.193"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            30..1247
                     /locus_tag="ACFM8U_001923"
     CDS             30..1247
                     /locus_tag="ACFM8U_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6335100.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTSIAIIGLANIAGCIF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            1244..1375
                     /locus_tag="ACFM8U_001924"
     CDS             1244..1375
                     /locus_tag="ACFM8U_001924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335101.1"
                     /translation="MKECRLKTLRRHFPNTPPVSGLHDTQISLIKPQTTAAIKPPFK"
     gene            complement(1450..1590)
                     /locus_tag="ACFM8U_001925"
     CDS             complement(1450..1590)
                     /locus_tag="ACFM8U_001925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335102.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPAQAGAASDGI
                     LS"
     gene            1603..2730
                     /locus_tag="ACFM8U_001926"
     CDS             1603..2730
                     /locus_tag="ACFM8U_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6335103.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLTTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDSRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGGDLERLAARDIIRFPGFVNNVSEVIKAH
                     HIFVLPSYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEK
                     MIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          659 a          834 c          663 g          633 t
ORIGIN      
        1 gcaaacttaa ccgactcaaa atacacatta tgacgcacac cgcatcgaaa acacccaaat
       61 tttggctgac catcaccgcc gccgcattca tcctgctgat taccatcggc atgaggatga
      121 cgctcggact gttcgtccaa cccgtcgtca acaccaccga attgagcatt gcccaattca
      181 gcctcatcat cgccgttttc caactgatgt ggggcatatc gcaaccattg tccggcgcgc
      241 ttgccgaccg tttcggcgcg ttcagggtat taagcggcgg cgcagtcctg ctcgtctgcg
      301 cctgcctgat tgcccccaac atcccgactt attgggggct gatgattgcc gtcgggctgc
      361 tgctcgcatt cggcacaggc tcaggcggtt tctccatcat tatggggcag gtcgccgccc
      421 aagtccccgc acacaaacgc ggcttggcat ccggactggt caacgccggc ggttcggcag
      481 gacaattcct gttcgcaccg ctggttcaag gactcgtcgt cctgcccgaa gtcggctgga
      541 cgggcacatt ttacgtttgg ggcgcaatcg ccctgctgat tctgcccgtc tcatggtggc
      601 ttgccggcgg caacaacgcc gcccacaccc aacacgccca agccacacac ggacaaagcc
      661 tcggagaagc agtcaaaacc gccttcaaaa ccccaagcta catcctgctg cacctaagct
      721 tttttgcctg cggcttccac atcgcctttc tcgtaaccca cctacccacg gaagtcgccc
      781 tgtgcggact gcccgccacc gtcgcctcga catccatcgc catcatcgga ctggcaaaca
      841 tcgccggctg cattttttcc ggctggtgca caagccgcgt tcggggcaaa tacgtcctgt
      901 tcggactgta cgcctcgcgc gccgcgatga tactcatcta cattttttcg cccaagaccg
      961 acctcaactt ctacattttc gccgccgcac tcggatttac ctggctcgcc accgtcacgc
     1021 cgaccgcctc cattaccggc aaactcttcg gcacacgcta cctcgccacc ctgttcggac
     1081 tgacgatgct cagccaccaa atcggcggat tcctcggctc gtacatcggc ggcatcgtga
     1141 ttacacaatt cggcgactac ggctggatgt ggtacgccga cgcactgctg gcgggaaccg
     1201 ccgccctcct caacctcccc atccgcgaac cccgaaccgc cgcatgaaag aatgccgtct
     1261 gaaaaccctc agacggcatt ttccaaacac cccgcccgtt tccggactgc acgacacgca
     1321 aatcagcctt atcaaaccgc aaacaaccgc cgcgattaaa ccgccattca aataagtccg
     1381 ttttcccttc gggtgtaaaa tcatcacaaa acgcgatact gtatatcgcc gggcgttatg
     1441 cagcggattt caggataaaa tgccgtctga agccgcccct gcctgggcgg gcgaagcgtc
     1501 ggatgcggtg cgcttggcaa aggtttgccc gccgcgcccc gtatccggca tttcatcaat
     1561 gcagaccgca tttccaatcc tataattcaa ggtgtccgcc ttatgaaaat cgtttttatc
     1621 acaacagtcg catccagcat ttacggtttc cgcgcccccg tcattaaaaa attaatcggc
     1681 aaaaaccatc aggtgtatgc ctttgtatcg gagttttccg ataatgaatt ggatattatc
     1741 agggaaatgg gggtaacacc cgttacctac cgttcaaacc gcagcgggct gaacccgttt
     1801 tccgatataa aatccacctt cctcatattt aaagaactca aaaaaatatc gccggatttg
     1861 gttttccctt atttcgcaaa acccgtgatt ttcggcactt ttgccgcaaa actggcaggc
     1921 gtgcccagaa tcgtcgggat gctggaaggt ttgggattcg catttacccc gcagccggaa
     1981 ggcataccgt taacaacaaa aatcataaag gggattttga ttgccttgta ccacattgcc
     2041 ctgccgatgt tggaaagcct gattgtatta aaccccgacg acaaagacga actgacggac
     2101 aaatacggca tcaaaataaa aaacatccat attttgggcg gaatcggtct ggattcgcgg
     2161 caatatcctt attccgaggc ggatattccc gatgaaaaag aacccgtaaa attcctcttt
     2221 atcggcagat ttctgaaaga aaaggggatt gatgatttta ttcgggcggc ggaacaggtt
     2281 aaggacaaat accccgatac ggtttttacc gctttgggcg caatcgacaa atcacgcggg
     2341 ggggggggcg atttggaacg gcttgccgcc cgcgatatta tccgtttccc cggttttgtg
     2401 aacaatgttt ccgaagtgat aaaggcgcat catatattcg tattgccgtc ttatagggaa
     2461 ggcgttcccc gaagcactca ggaggcaatg gccgtcggca gggcagtgat tacgacggat
     2521 gtccccggat gcagggaaac ggtcgccgac aaggtcaacg gcttcctgat cgagccttgg
     2581 aatccccgca tcttagccga aaaaatgatt tattttatcg aaaacaggga agccgtccgc
     2641 ctgatgggga atgcaagtta tgcgattgcc aaagataaat tcgatgccga aaaagtcgat
     2701 ttgaaattgc tcgatatttt gaaggcgtaa acaaggctgc ccgcttttga gtttcggtca
     2761 tttctgataa acccccgtca ttcccgcca
//
LOCUS       DAXHSJ010000194         2744 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000194 DAXHSJ010000000
VERSION     DAXHSJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.194"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..137)
                     /locus_tag="ACFM8U_001927"
     CDS             complement(<1..137)
                     /locus_tag="ACFM8U_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6335104.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YL"
     gene            complement(207..425)
                     /locus_tag="ACFM8U_001928"
     CDS             complement(207..425)
                     /locus_tag="ACFM8U_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335105.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            438..608
                     /locus_tag="ACFM8U_001929"
     CDS             438..608
                     /locus_tag="ACFM8U_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFC6335106.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(653..958)
                     /locus_tag="ACFM8U_001930"
     CDS             complement(653..958)
                     /locus_tag="ACFM8U_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335107.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTTAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTVTLLEHAAEFGGYVSELENQNQAWRDWAGNHSRKVGN"
     gene            complement(855..1274)
                     /locus_tag="ACFM8U_001931"
     CDS             complement(855..1274)
                     /locus_tag="ACFM8U_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335108.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYNRALGYGN"
     gene            complement(1246..1482)
                     /locus_tag="ACFM8U_001932"
     CDS             complement(1246..1482)
                     /locus_tag="ACFM8U_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335109.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
     gene            complement(1485..1622)
                     /locus_tag="ACFM8U_001933"
     CDS             complement(1485..1622)
                     /locus_tag="ACFM8U_001933"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335110.1"
                     /translation="MIELWSSKSREASEQADLAAFEFAEGELANYQEMLKRHLQTKSV
                     E"
     gene            complement(1652..1858)
                     /locus_tag="ACFM8U_001934"
     CDS             complement(1652..1858)
                     /locus_tag="ACFM8U_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335111.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRH"
     gene            complement(1855..2400)
                     /locus_tag="ACFM8U_001935"
     CDS             complement(1855..2400)
                     /locus_tag="ACFM8U_001935"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6335112.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGRHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            complement(2556..>2744)
                     /locus_tag="ACFM8U_001936"
     CDS             complement(2556..>2744)
                     /locus_tag="ACFM8U_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HFC6335113.1"
                     /translation="KALIQLRNHRFYQEFVKLRDIDKESGLMAMTKLCPKYGISSRTG
                     YTIINEMSRPAAQQAALF"
BASE COUNT          628 a          758 c          626 g          732 t
ORIGIN      
        1 aggtaacggg ttttttgacg gttcaggtat tgttcgatcg gctgccaatc aatcacctgg
       61 tccaacttca atagtgggaa gcggtcgatg tgtttagcaa tcatggcttg tgcggtttgc
      121 cggaagaagg tgctcatgag aaatctccta aatgtcttgg tgggaattta ggggattttg
      181 gggaattttg caaaggtctc ggtaggtcat tctttgatac ccattaaata ttttgtccgc
      241 ttgtacaatt tcttaaccca cgaaatattt acaaatgtat aaacctttgc tacaacttcg
      301 ccgtcatagt gcgcattttc tcgtgcagcc cgaaatttcg ccctggcttc ttcagggttg
      361 tccgcccaaa tgctcaatga ccaggacttg ccgttaaagc ggtaagaaaa cgtgtactca
      421 ttcataggag aaaccttatg tattttgaaa tctataaaga cgcaaaaggc gaataccgtt
      481 ggcgtttgaa agcagccaac catgaaatca tcgctcaggg cgaaggctac accagcaagc
      541 aaaactgtca gcacgcagtc gatttgctga aaagcactac cgccgctacc cctgtaaaag
      601 aggtataaaa tccgctttca ccctcagccc gcgccctacg cgggcttttt tgtcagtttc
      661 cgactttgcg ggagtgattg cccgcccagt cgcgccaagc ctgattttgg ttctccagtt
      721 ccgaaacata gccgccaaac tcagcggcgt gttccaacag cgttactgtc ttgccgtctt
      781 tcggcggatt cgggcgtacc ggcgcaacca tcaatgcggc aggcggtgtc ggcatgaccg
      841 ccttttcgac aaccttaatt tccgtagccg agggcgcggt tgtagagctg caagccgtga
      901 tggccaaagc cgtcaataca accgccgcct gcatttttac ggtcttgagt aaggacattt
      961 tcgatttcct ttttattttc cgttttcaga cggctgactt ccgcctgttt tttcgccaaa
     1021 gccatgccga cagcgtgcgc cttgacttca tattttttag cttccgcacg cgcctgttcc
     1081 agctcgcggg cgtagttttg agccgacaac agcagggctt gcgctttgtc tttttccatc
     1141 ttgtcaatga ccgcctgctg cttcgcaaaa gccgacttgt agccttgatg gtgcgacacc
     1201 gccaagagag tgccgacaag cgcgatgatg gcaatcggtt gccagttatt cgccagcagt
     1261 ttcacgagat tcattctcga cctcctgacg tttgacgctg accaacgaac gcgccaccgc
     1321 atagccgccg acaatgccca aatacaccgc ccaaatctcc gccgacgggt cgggcagcat
     1381 cacaaactta accgtccccg ccgcgcaggc aacgtttgcc cacagtttcg agtgcgacac
     1441 attgcctgtc gccgggtttt taaaaatatc caaaatacgc attgctattc cacacttttg
     1501 gtttgcaggt gccgtttcag catttcctga taattggcca gttcgccctc cgcaaattcg
     1561 aacgcagcca agtctgcctg ttcgcttgcc tcgcggcttt tgctcgacca tagctcaatc
     1621 atcttttcgt aaaacgcaac ctgtcccatg attaatgacg attcttgcgt ttgcgcgccg
     1681 cacgtttcgc ggctgccacg cctgagtttc ttaaatttgg gcgtggatag gtgtgtatcg
     1741 gacgaacatt tggcatctgt ataagtgcgc ggtttacctg ctcattacta ggattttctc
     1801 caatcaattt tgtaagaaaa taggaagcag cttctttcaa tttaccaata atattcatac
     1861 tcttgccgcc cccaattcca tcgcaatcgc gtccgcaatc gcgcggcaga tgccccattt
     1921 ggtcgtctta aacaaggcca aatcagtgtc gttgctgata aaaaacggct caaacacaat
     1981 gccgccggct tgcgcataag ccaggcgcga atgccgaccc gcattgtcgg gtttaaagcc
     2041 gtcttcgccg cgcagtttcc agccggtttt cttggcaacg gctttgctca acacctgaca
     2101 ccagcgtttg tttttaacgg tactcaaggc ttcgatgcct gtcgccgctt tgctgacggc
     2161 agcgttggtg tgaaactcaa tcgccacatc cgagccgcga atcagtttta cggcttcacg
     2221 cagcggcata ttgcctttgc ctgtgccgtc ggttttaaca gtcaaaccgt agtcatcgcg
     2281 caaaatagca gccacgatat tgcgcatatc ctgcgccaag tccgcctcac ggtcgctgcc
     2341 gttgaccgca cccgggtcgg tgttgctgtg tccagcggtt aaggttacgg ttttgcccat
     2401 tcatcatctc caaaaaaggt tgattgcata acatttgaaa accccattaa accgtcttta
     2461 acccatcgtc ccgaacggta gatgtttcat cccctgcaaa ccaaaaaaaa gaccgtctga
     2521 atacagacgg ccaaagcctg gtcacacatc actgcctaaa ataaagctgc ctgctgtgcc
     2581 gcaggtcggc tcatttcatt gataatcgta tatcccgttc gtgaagagat gccgtattta
     2641 gggcatagct tcgtcatcgc cataagcccg ctctccttat caatatcgcg caatttgaca
     2701 aactcctgat aaaacctatg gtttctcaac tgtattaacg cctt
//
LOCUS       DAXHSJ010000195         2702 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000195 DAXHSJ010000000
VERSION     DAXHSJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.195"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            778..2556
                     /gene="nhhA"
                     /locus_tag="ACFM8U_001937"
     CDS             778..2556
                     /gene="nhhA"
                     /locus_tag="ACFM8U_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin NhhA"
                     /protein_id="HFC6335114.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVETAVLATLLF
                     ATVQASANNEEQEEDLYLDPVQRTVAVLIVNSDKEGTGEKEKVEENSDWAVYFNEKGV
                     LTAREITLKAGGNLKIKQNGTNFTYSLKKDLTDLTSVGTEKLSFSANGNKVNITSDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKKRAASVKDV
                     LNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKKTEVKIGA
                     KTSVIKEKDGKLVTGKGKDENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVKYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSV
                     SLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          832 a          655 c          649 g          566 t
ORIGIN      
        1 caacgatgta ctggtttttg ttaatccact atatccgttt gcgcccgcca agccgtgttg
       61 cacagatgcg acacggcttt tttctttccg ttgtcagaat gtaaattttc gtcaaaaaac
      121 atcaaaaatc accacccccc cgaatttgcg taaaataaag tgtaatccct ttgattataa
      181 gggaatgttg acaattagat cgattctcct aggctgactt tcgcaataaa tggggttgtc
      241 aataattgtg tatggtcgtt tgccgtccgg cggccgtcat tcccgcgcag gcgggaatcc
      301 agcaacacaa cgcggcagga ctttatcgga aaaaacagaa accccaccga ccgtcattcc
      361 cgcaaaagcg ggaatccaga cccgtcggca cggaaactta ccggataaaa cagtttcctt
      421 agattccacg tcctagattc ccgccttcgc gggaatgacg agattttaga ttatgggaat
      481 ttatcaggaa tgattgaatc catagaaaaa ccacaggaat ctatcagaaa aaacagaaac
      541 cccaccgccg tcattcccgc aaaagcggga atccagaccc gtcggcacgg aaacttaccg
      601 aataaaacag tttccttaga ttccacgtcc cagattcccg ccttcgcggg aatgacgaga
      661 ttttaagttg ggggaattta tcagaaaacc cccaaccccc aaaaaccggg cggatgccgc
      721 accatccgcc cccaaacccc gatttaacca ttcaaacaaa ccaaaagaaa aaacaaaatg
      781 aacaaaatat accgcatcat ttggaatagt gccctcaatg cctgggtcgt cgtatccgag
      841 ctcacacgca accacaccaa acgcgcctcc gcaaccgtgg agaccgccgt attggcgaca
      901 ctgttgtttg caacggttca ggcaagtgct aacaatgaag agcaagaaga agatttatat
      961 ttagaccccg tacaacgcac tgttgccgtg ttgatagtca attccgataa agaaggcacg
     1021 ggagaaaaag aaaaagtaga agaaaattca gattgggcag tatatttcaa cgagaaagga
     1081 gtactaacag ccagagaaat caccctcaaa gccggcggca acctgaaaat caaacaaaac
     1141 ggcacaaact tcacctactc gctgaaaaaa gacctcacag atctgaccag tgttggaact
     1201 gaaaaattat cgtttagcgc aaacggcaat aaagtcaaca tcacaagcga caccaaaggc
     1261 ttgaattttg cgaaagaaac ggctgggacg aacggcgaca ccacggttca tctgaacggt
     1321 atcggttcga ctttgaccga tacgctgctg aataccggag cgaccacaaa cgtaaccaac
     1381 gacaacgtta ccgatgacga gaaaaaacgt gcggcaagcg ttaaagacgt attaaacgca
     1441 ggctggaaca ttaaaggcgt taaacccggt acaacagctt ccgataacgt tgatttcgtc
     1501 cgcacttacg acacagtcga gttcttgagc gcagatacga aaacaacgac tgttaatgtg
     1561 gaaagcaaag acaacggcaa gaaaaccgaa gttaaaatcg gtgcgaagac ttctgttatt
     1621 aaagaaaaag acggtaagtt ggttactggt aaaggcaaag acgagaatgg ttcttctaca
     1681 gacgaaggcg aaggcttagt gactgcaaaa gaagtgattg atgcagtaaa caaggctggt
     1741 tggagaatga aaacaacaac cgctaatggt caaacaggtc aagctgacaa gtttgaaacc
     1801 gttacatcag gcacaaatgt aacctttgct agtggtaaag gtacaactgc gactgtaagt
     1861 aaagatgatc aaggcaacat cactgttaag tatgatgtaa atgtcggcga tgccctaaac
     1921 gtcaatcagc tgcaaaacag cggttggaat ttggattcca aagcggttgc aggttcttcg
     1981 ggcaaagtca tcagcggcaa tgtttcgccg agcaagggaa agatggatga aaccgtcaac
     2041 attaatgccg gcaacaacat tgagattacc cgcaacggta aaaatatcga catcgccact
     2101 tcgatgaccc cgcagttttc cagcgtttcg ctcggtgcgg gggcggatgc gcccacttta
     2161 agcgtggatg acgagggcgc gttgaatgtc ggcagcaagg atgccaacaa acccgtccgc
     2221 attaccaatg tcgccccggg cgttaaagag ggggatgtta caaacgtcgc acaacttaaa
     2281 ggtgtggcgc aaaacttgaa caaccgcatc gacaatgtgg acggcaacgc gcgtgcgggc
     2341 atcgcccaag cgattgcaac cgcaggtctg gttcaggcgt atctgcccgg caagagtatg
     2401 atggcgatcg gcggcggcac ttatcgcggc gaagccggtt atgccatcgg ctactcaagc
     2461 atttccgacg gcggaaattg gattatcaaa ggcacggctt ccggcaattc gcgcggccat
     2521 ttcggtgctt ccgcatctgt cggttatcag tggtaagggc ttttcgcctg tctgctgttt
     2581 gggataggcg gaaggtttga agggaagggt ggcgatttgc cgcctgagac ctttgcaaaa
     2641 ttccccaaaa tcccctaaat tcccaccaag acatttaggg gatgttccat gagcaccttc
     2701 tt
//
LOCUS       DAXHSJ010000196         2622 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000196 DAXHSJ010000000
VERSION     DAXHSJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.196"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            77..640
                     /gene="gmhB"
                     /locus_tag="ACFM8U_001938"
     CDS             77..640
                     /gene="gmhB"
                     /locus_tag="ACFM8U_001938"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC6335115.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTGADGCGCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            670..1413
                     /locus_tag="ACFM8U_001939"
     CDS             670..1413
                     /locus_tag="ACFM8U_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6335116.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            1410..2102
                     /locus_tag="ACFM8U_001940"
     CDS             1410..2102
                     /locus_tag="ACFM8U_001940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6335117.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CHKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPKAKQW
                     LKIHGRELFALG"
BASE COUNT          624 a          879 c          634 g          485 t
ORIGIN      
        1 acgcggcaca ggcgcatcac tgccgatatt tcagtataat gccacccccg acctgcccca
       61 atccaaagga aacgcgatga aactcatcat tctcgaccgc gacggcgtca tcaatcagga
      121 ccgcgacgac ttcgtcaaat ccgttgacga gtggatacct gtcgaaggca gcatggatgc
      181 ggtggcattt ctgacgcagg caggctacac cgtcgccgtt gccaccaacc aatccggcat
      241 cgggcgcaaa tattttaccg ttcaaaacct caccgaaatg catgccaaaa tgcaccgcct
      301 cgtccgtcag gcaggcggcg aaatcaacgg catctggttc tgccctcata ccggcgccga
      361 cggctgcggc tgccgcaagc ccaaaccggg tatgattgaa gacatcctcg aacgcttcaa
      421 cgcccaagct tcggaaactt ggctggtcgg cgacagcctg cgcgatttgc aggcaatcga
      481 tgccgtcggc ggcaaacccg cgctggttct gaccggaaaa ggcaaaaaaa cgctctccca
      541 acacggacac gaattgcccg aacacacaca ggttttcgat accttgctcg atttctcaca
      601 atacatcatg caggaaaacg ccgcaccgca agccgactga acataccgca tttccgacaa
      661 ggcaaaacca tgctcatcat ccgcaacctg atttactggc tgatactctg ttccaccctg
      721 attttcctct ttccctttat gctgctcgcc tcgcctttcc gagacggggc gcacaagatg
      781 gcgcgggtct gggtcaaaat cctcaacctc tcgctcaaac acatcgtcgg gctcaaatac
      841 cgcatcatcg gcgcggaaaa catccccgac cgccccgccg tcatctgcgc caaacaccaa
      901 agcggctggg aaacgctcgc ccttcaggac atttttccgc cgcaggttta cgttgccaaa
      961 cgcgagttgt tcaaaatccc ctttttcggc tggggcttga aactggtcaa aaccataggc
     1021 atagaccgca acaaccgccg cgaagccaac gagcagctca taaaacaggg gttggcgcgc
     1081 aaaaacgaag gctattggat taccattttc cccgaaggca cgcgccttgc acccggaaag
     1141 cgcggcaaat acaaactcgg cggcgcgcgc atggcgaaaa tgtttgagat ggacatcgtc
     1201 cccgtcgccc tcaacagcgg cgaattttgg ccgaaaaact cctttctgaa atatccgggg
     1261 gaaatcaccg tcgtcatctg tccgaccatc ccgcacgcaa gcggcagcga agccgaattg
     1321 atggaaaaat gcgaacacct catcgaaacg cagcagccgc tcatttccgg cgcaggcccg
     1381 tttgccgcca aaatgccgtc tgaaaccgca tgaccgcctt tgtccacacc ctttcagacg
     1441 gcatggaact gaccgtcgaa atcaagcgcc gtgccaagaa aaacctgatt atccgccccg
     1501 ccggcacaca taccgtccgc atcagcgtcc caccctgctt ctccgtctcc gctctaaacc
     1561 gctggctgta tgaaaacgaa gccgtcctgc ggcaaacact ggcgaaaaca ccgccgccgc
     1621 aaactgccga aaaccggctg cccgaatcca tcctcttcca cggcagacag cttgccctta
     1681 ccgcccatca agacacgcaa atcctgctga tgccgtctga aatccgtgtt cccgaaggcg
     1741 cacccgaaaa acagcttgcg ctgctgcggg actttttgga acggcaggcg cacagttacc
     1801 tgattccccg cctcgaacgc cacgcccgca ccacacaact gttccccgcc tcctcctcgc
     1861 taacctctgc caaaaccttc tggggcgtgt gccacaaaac cacaggcata cgcttcaact
     1921 ggcggctggt cggcgcaccg gaatacgttg ccgactatgt ctgcatacac gaactctgcc
     1981 acctcgccca tccagaccac agccccgcct tttgggaact gacccgccgc ttcgccccct
     2041 acacgcccaa agcgaaacag tggctcaaaa tccacggcag ggaacttttc gccttaggct
     2101 gacgcggccc gggcaggcat ccgccaattc agacctcctc ccaaacggac atccggaaag
     2161 cagcatcaca gccggcgaca cataccgctg cctccgcttt cagacggcat ccgtgccgga
     2221 acaggcacgc gcccgcccga ttcaaaccgc gatgacgctt tgccgccggt tcggggcagg
     2281 atggcggcac acgcgccgtc tgccgcgttt catttcacac cgctcttccg aaacccgaaa
     2341 cccgcccggt ccgacgtgcg gtatgaaacg cttaagctga cgcgaagtct tttactgatt
     2401 tgcccgcgaa aatgccgtct gaaaggtttt cggacggcat ttttttgcgt ttcccaggat
     2461 ggcggcggat tcgtaaaagg cggtcagggt ggattgtagg atgggttgag acctgccgaa
     2521 tccgccgcat ctgccaaatc taccgccgtc attcctacga aagtgggaat ctagaacgcg
     2581 gggttaagaa aacctgcatc ccgtcattcc cacgaaagtg gg
//
LOCUS       DAXHSJ010000197         2619 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000197 DAXHSJ010000000
VERSION     DAXHSJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.197"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(81..359)
                     /locus_tag="ACFM8U_001941"
     CDS             complement(81..359)
                     /locus_tag="ACFM8U_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335118.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            complement(366..584)
                     /locus_tag="ACFM8U_001942"
     CDS             complement(366..584)
                     /locus_tag="ACFM8U_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC6335119.1"
                     /translation="MKFINTCRKYGAKLAVVTAAPLALAAHANATLPDTAKNALEAAK
                     ADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            complement(658..855)
                     /locus_tag="ACFM8U_001943"
     CDS             complement(658..855)
                     /locus_tag="ACFM8U_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335120.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(860..1150)
                     /locus_tag="ACFM8U_001944"
     CDS             complement(860..1150)
                     /locus_tag="ACFM8U_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335121.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            complement(1195..2538)
                     /locus_tag="ACFM8U_001945"
     CDS             complement(1195..2538)
                     /locus_tag="ACFM8U_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC6335122.1"
                     /translation="MEDKQGMTKAVVGVMTDAPADGRKPATASNLPPLSNRGGTETET
                     AGRVQEVFECYETYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYGVRVS
                     LLEDEDFIRAASMLAEEVFGFGLYKESKGSGGRFYERCWLMGSEDVLYGRVHFGGQNN
                     TILFELTGTGCGVAKEGWESRLFAFLTNAIRPKITRVDIAKDFFNGEYSPNQAREDRN
                     KGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLGDKT
                     STWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFAQKATRIHAVKEDKVISAD
                     RYLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLAPEAYDCAFLKAQA
                     IHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
BASE COUNT          637 a          613 c          600 g          769 t
ORIGIN      
        1 aacttcatgc gctcaattct taaacagaac cgccccgatt aatacgggta cggaaacgcc
       61 gagataaaaa taaaaatcca tcatttcaaa acctttttca gcagggaaac aaagtaaacg
      121 gacgcgagga tgccgaatac tatccagcct gtttcaagac cgctttgcag gttgtctttc
      181 ggactgcatt ccgccaataa aagccttagc ggctgaccgt ccgacatctt ccacaggctg
      241 ccgttatatt ccggcctgac aatctgtccg ttttctttga ttcttggtac taccaagctg
      301 aaataaaggt tttcagcctg gtgcttctca agacatttat ttccgacttg gtagtacatg
      361 ccgtcttact tcatcactct cttaacgatg gaaaatacaa aaagcgcggc gaaaacgccc
      421 actacaatcc aaccggcttc cataccgtcg gcttttgcgg cttccaaagc gttttttgcc
      481 gtatcgggca acgttgcatt tgcatgtgcg gccaaagcca ggggagcagc tgttacaaca
      541 gccagttttg caccgtattt acggcaggtg ttaataaatt tcatgatatt ttccttcaaa
      601 aagtgtttgg cggtaatgga tggagcgttt ttcagacgac cgccgaacat ccgaaaatca
      661 gtctttcaaa aatccgaata cgacaaattc gtattggttg ccgatttctt ccaaacctgc
      721 gttaatcgct tcttcgaagt cgtagaaata atcggcattg gtgattaatt tggtatgtcc
      781 gatgtcgccc gtttcaggag agagatacag aaagtcccct gttgatacgg actggacaac
      841 atagactttc tgcattcaat cagcctttct tcacgagttg aaaaccgatg actttcagtt
      901 tttgggtttt gcccgtagtg acgatttcta cgttcaggtt tgcttcgatc ggaaattggg
      961 cgtttcggaa ctgctcgaaa ttggcagagc cgccgaaatc gtattcagta gtagagctgc
     1021 ccaatgcgtt gccttgggag ctgtctaagg gtgtggcgac aatcaggcgg caatagtcga
     1081 agctcttgcc ttcgatttgt ccgttgaatt ttttaacgcc gacgatgtgg ccttgaagtt
     1141 ggatgttcat tttttggttt ccttgtgtga ttaaacgtct ttcgggcaga cactttaagc
     1201 ccatgaaatc ggtagtcttg cgaatttgtc gtaaatgaag ttgttatagc tttcttcatt
     1261 gttgacgtgt ttttgctgtt caagctgttt ttcaagattc tcgtaatatt cgtacatata
     1321 gtaagggtct ttgtacggtt tgaatgcggg ctgttcatga atggcttgag ctttcaaaaa
     1381 ggcgcagtcg taggcttcgg gagccaaaga cttgggcaac ttgtgatgac tcggctcaat
     1441 cagttcaaac agtttggctt tgtccaattc gggaaaaatg aatttcagac cgtttgccgc
     1501 acgtccgaac tgttttttta cccattcaag gtagcggtcg gctgaaatga ccttatcttc
     1561 cttaaccgcg tgtatgcgcg ttgccttttg ggcgaatcgt tcgcaaatcg gatatgcgcc
     1621 gccgaaatat tcgcccggat tctgcaaaac ttcgaaaggg ataacgatgt cttttgcttt
     1681 gaattcaatt tcaaagcgcg tccatgtgct tgttttatcg cccaactgct tgcctttttc
     1741 atagacgcgg acatatttgg acgattcacg ggagccgata ccataggtct tgcctttggt
     1801 cattttggct tcatcgtctt cttcccaatc tgaccccaaa cattcgcctt ttggtttgac
     1861 gtgatgacag gtaaacatac ctttatttcg gtcttcacgg gcttggttcg ggctgtattc
     1921 gccgttgaaa aagtcttttg cgatgtcaac gcgtgtgatt tttgggcgga ttgcattagt
     1981 cagaaatgcg aaaagtcgtg attcccagcc ttcttttgcg acgccgcaac cggtgccggt
     2041 cagttcgaaa aggatggtat tgttttgtcc gccaaaatgg acgcgaccgt ataggacgtc
     2101 ttccgaaccc atcaaccaac agcgctcata aaaacgaccg cccgagcctt tggactcttt
     2161 gtagagaccg aaaccgaaaa cttcttcggc gagcatggac gcggcgcgaa taaaatcttc
     2221 gtcttccaaa agacttacac gaacgccgta tttatcgaaa aaggtttttt catgaaatga
     2281 aaagctaatt tgatcaatga aagccgaatc tgatacaccg cgccgaagag gaacgcctaa
     2341 caggtttcct ttaccgtccg ttatgtacgt ttcgtaacat tcgaagactt cctgaaccct
     2401 gcctgccgtt tcggtttctg taccccccct gttagataag gggggaaggt ttgaagcggt
     2461 tgccggcttc ctgccgtccg ccggtgcgtc cgtcatcacg ccgacaaccg cctttgtcat
     2521 cccttgctta tcttccatgg tgcgaatcct caaaaacggg caaaaaaaaa gccctgttac
     2581 ttgtagaaag taaaggacgt taatttttgt taatcgtcc
//
LOCUS       DAXHSJ010000198         2612 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000198 DAXHSJ010000000
VERSION     DAXHSJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.198"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..200
                     /locus_tag="ACFM8U_001946"
     CDS             <1..200
                     /locus_tag="ACFM8U_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6335123.1"
                     /translation="FAERQNHINGIENFWNQAKRHLRKFNGIPKAHFELYLKECEWRF
                     NNSEIKVQISILKQLVKSSLS"
     gene            399..1019
                     /locus_tag="ACFM8U_001947"
     CDS             399..1019
                     /locus_tag="ACFM8U_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6335124.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            1042..2493
                     /locus_tag="ACFM8U_001948"
     CDS             1042..2493
                     /locus_tag="ACFM8U_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6335125.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
BASE COUNT          589 a          599 c          613 g          811 t
ORIGIN      
        1 attttgctga acgacaaaac catattaatg gaattgagaa cttttggaac caggcaaaac
       61 gtcatttacg caagtttaac ggcattccca aagcgcattt tgagctgtat ttaaaggagt
      121 gcgaatggcg ttttaacaac agtgagataa aagttcaaat ttccatttta aaacaattag
      181 taaaatcgag tttatcttag ttgtccagga cagccccatt atttttataa caccgtgaag
      241 ccgcacagca gtttgaacag tgatacgccg tttgcgggct tacgagttta ttttcccggc
      301 ctgcagtttg agcaatacgg tgatttccta cggttaatac aaatgtttac acattgatac
      361 atttcattta tagttccgcc tatttgaaaa tagaaaatat gaattcgacc gcaagtaaaa
      421 ccctgaaagg attgtcgctg gtgtttttcg cctctggatt ctgcgccctg atttaccagg
      481 tcagctggca gaggcttcta ttcagtcaca taggtatcga tttgagttcg attactgtca
      541 ttatttctgt atttatggtc ggcttgggtg taggtgcgta tttcggtgga cgcattgctg
      601 accgttttcc ttcaagtatc atccccctgt tttgcatcgc tgaagtatcc atcggtctgt
      661 tcggtttggt aagcaggggt ctgatttccg gcttggggca tcttttagtt gaggctgatt
      721 tgcccatcat cgctgctgcc aatttcctct tattgctgct tcctaccttt atgatgggcg
      781 cgaccttgcc cttgctgacc tgttttttta accggaaaat acataatgtt ggcgagtcta
      841 tcggtacctt atattttttc aacactttgg gtgcggcact cggatcgctt gccgccgccg
      901 aatttttcta cgtctttttt accctctccc aaaccattgc gctgacagcc tgctttaacc
      961 ttctgattgc tgcttcagta tggctgcgtt acagaaagga tggatatagt gaacactaaa
     1021 ccgaatacta gtttgattta tatgctttct ttccttagcg gcttattgag cttgggtata
     1081 gaagtcttgt gggtaaggat gttttcgttc gcagcacagt ccgtgcctca ggcattttca
     1141 tttactcttg cctgttttct gaccggtatc gccgtcggcg cgtattttgg caaacggatt
     1201 tgccgcagcc gctttgttga tattcccttt atcgggcagt gcttcttgtg ggcgggtatt
     1261 gccgactttt tgattttggg tgctgcgtgg ttgttgacgg gtttttccgg cttcgtccac
     1321 cacgccggta tcttcattac cctgtctgcc gtcgtcagag ggttgatttt cccgctcgta
     1381 caccatgtgg gtacggatgg caacaaatcc ggacgacagg tttccaatgt ttatttcgcc
     1441 aacgttgccg gcagtgcatt gggtccggtc cttatcggct ttgtgatact tgatttcttg
     1501 tccacccaac agatttacct gctcatctgt ttgatttctg ctgctgtccc tttgttttgt
     1561 acactgttcc aaaaaagtct ccgactgaat gcagtgtcgg tagcagtttc cctaatgttc
     1621 ggcatcctca tgttcctact gccggattct gtctttcaaa atattgctga ccgtccggat
     1681 aggctgattg aaaacaaaca cggcattgtt gcggtttacc atagagatgg tgataaggtt
     1741 gtttatgggg cgaatgtata cgacggcgca tacaataccg atgtattcaa tagtgtcaac
     1801 ggcatcgaac gtgcctatct gctaccctcc ctgaagtctg gcatacgccg cattttcgtc
     1861 gttggattga gtacaggttc gtgggcgcgc gtcttgtctg ccattccgga aatgcagtcg
     1921 atgatcgttg cggaaatcaa tccggcatac cgtagcctta tcgcggacga gccgcaaatc
     1981 gccccgcttt tgcaggacaa acgtgttgaa attgtattgg atgacggtag gaaatggctg
     2041 cgtcgccatc ctgatgaaaa attcgacctg attttgatga atacgacttg gtactggcgt
     2101 gcctattcca ccaacctgtt gagtgcggaa tttttaaaac aggtgcaaag ccaccttacc
     2161 ccggatggta ttgtaatgtt taataccacg cacagcccgc atgcttttgc taccgccgta
     2221 cacagtattc cctatgcata ccgctatggg catatggtag tcggctcggc aaccccggta
     2281 gttttcccta ataaagaact gctcaagcaa cgtctctccc ggttgatttg gccggaaagc
     2341 ggcaggcacg tatttgacag cagcaccgtg gatgctgcag cacaaaaggt tgtctctcgt
     2401 atgctgattc agatgacgga accttcggct ggggcggaag tcattaccga cgataatatg
     2461 attgtagaat acaaatacgg cagagggatt taaccgtctt aaagggtttc aggcaacgca
     2521 ggttttaggt aacgtcctgc tagttcaaaa aaaaccgcat cacagcagtc gggacaaaat
     2581 ggtttaaaca ttttgtcccg aattcttatt cc
//
LOCUS       DAXHSJ010000199         2580 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000199 DAXHSJ010000000
VERSION     DAXHSJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.199"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(196..267)
                     /locus_tag="ACFM8U_001949"
     tRNA            complement(196..267)
                     /locus_tag="ACFM8U_001949"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(232..234),aa:Arg,seq:cct)
     gene            complement(287..733)
                     /locus_tag="ACFM8U_001950"
     CDS             complement(287..733)
                     /locus_tag="ACFM8U_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC6335126.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(738..1712)
                     /gene="ispB"
                     /locus_tag="ACFM8U_001951"
     CDS             complement(738..1712)
                     /gene="ispB"
                     /locus_tag="ACFM8U_001951"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFC6335127.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1936..2244
                     /gene="rplU"
                     /locus_tag="ACFM8U_001952"
     CDS             1936..2244
                     /gene="rplU"
                     /locus_tag="ACFM8U_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC6335128.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2269..2541
                     /gene="rpmA"
                     /locus_tag="ACFM8U_001953"
     CDS             2269..2541
                     /gene="rpmA"
                     /locus_tag="ACFM8U_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC6335129.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          755 a          696 c          575 g          554 t
ORIGIN      
        1 ataaaacaac agatttccaa gccggaacat cttttacgga gcctgaaaat catttcatga
       61 ttctaccgtc ctaaaggtcg gtttttcaag caggaagaaa aagttttcag atggcaaaaa
      121 agccctccaa cactgaaagg ccttatatcg gaaacttccc gcaacacggg aaacagacaa
      181 atgcaaatcg tcaaacctcg ccaacaggaa tcgaacctgt attttacgct taggaggcat
      241 acgttctatc cgttgaacta tggcgagccg aaatgaaaag gagattttaa ccctttccga
      301 cgacaaaaga caagatgccg gccgcaatca gcggcccgac agggataccg cccataaatg
      361 ccaccccgat aaccgtcccg attaacagcc ctgtaattaa aacaggctgc tgtcccatca
      421 aaggcacgcc gcgtcccgcc agccaagcca cgaaaatacc gataaaaacg gcggatatca
      481 ttttaaaatt caaaaattcg gcaacgggag gaacctgcgc ctttcctgaa accaacggac
      541 tcaaaacccc tatggtcaaa agaatgatac cgagattcaa cccgtgcttc tcgaccaacg
      601 ggacaaactg taccaatgcc gtctgctgca tcagcagcaa tatggttgcc gagatggtaa
      661 tcgaattgtt gttgctgacc acccccaaca gaatcagcgt aaccagaaac aggggaacaa
      721 aactgaaatt catcgcctca agacaccctg accaaagatt ccttcgccag ctgaatcatg
      781 gcatccttca cttcgctgtc gggcagggca tccaacgcgg caacggcaca atcgactgct
      841 ttgcgcgcct cgcctatcga atatgccaac gcatccgaac ggacgacata atcgtggatt
      901 ttctcaaaat agctgcgatc tgcattttcc aaagcagtac gcacatcgtt cgcaacctgt
      961 tcggaaccct gacgcatcag ataaatcaaa ggcaaagtcg gttttccttc cgccaaatcg
     1021 tcgccgacgt ttttgccggt ttcttcggtt tcgccagaat agtccagcac atcgtcaata
     1081 atttggaatg ccgtgccgac gtacataccg tagtctttca acgcctgttc gtgttcgggg
     1141 gaagccttgc ccaaaattgc gccgacttga gcggcagctt caaacaattt tgccgtttta
     1201 tattggataa cttggatata ttgttcttcg gtaatgtccg tattgccgat gttcatcagc
     1261 tgcatgactt cgccctcggc aataatgttg gttgcatccg ccatcacttc caaaacgcgc
     1321 atactgcccg aggcaaccat cagttgaaag gcgcgcgtgt ataaaaagtc gccaaccaac
     1381 acagccgccg cattgccgaa cagattgttt gccgttgccc gcccacggcg caaatcgctt
     1441 tcatcgacga catcgtcgtg caggagggtg gaagtgtgga taaactcgac catcgccgcc
     1501 agcgaataca gtttctcgtc atcataaccg accgccttac ccgccaaaat cgtcataatc
     1561 ggacgcaggc gtttgccgcc cgcgctgatg atatatgtac cgatttgcga aatcagtgcg
     1621 acatcggatt gcaccgcacg gttgatgact tcattgactt tggcaaggtc ttcaggcaga
     1681 tgtcgctgga aatagggcag attctcgagc atggcagact ctcaggttta cgtttccgtc
     1741 aaacggcaga taccgcttga aagctgattc aaatagcggg caattatatc agcaaagcca
     1801 tcagggtaca tcttttcaga cggcatatgg ttatccgaac cgcgcaaact ttgacaaaac
     1861 aggcaaataa caatacaatg ccgagttccg cacaatggtg tgcggatatt aaccataacc
     1921 cttatggagt tgagtatgta cgcggtcgta aaaaccggcg gcaaacagta taaagtttcc
     1981 gtcggcgaaa aattgaaagt agaacagata ccagccgaac tcgacagcca aatcgaactg
     2041 accgaagttt tgatgattgc tgacggcgaa tctgtaaaag ttggcgcacc ctttatcgaa
     2101 ggtgcaaaag taacggctaa agtagtggca cacggtcgtg gcgaaaaagt ccgcatcttc
     2161 aaaatgcgcc gccgcaaaca ctaccaaaaa cgccaaggcc accgccaaaa tttcacccaa
     2221 atcgaaatcg tggcaatcgc ctaaccttaa aacttaggag cattacaaat ggcaagtaaa
     2281 aaagcaggcg gcagcacccg caacggtcgc gattcagaag ccaaacgctt gggcgttaaa
     2341 gcctacggca acgaactgat tcccgcaggt tccatcatcg tacgccaacg cggtaccaaa
     2401 ttccacgcag gcgacaacgt aggtatgggc aaagaccaca ctttgttcgc caaagttgac
     2461 ggttatgtcg aattcaaaac caaaggcgcg ctgaaccgta aaactgtcag catccgtcct
     2521 tacaccggtt ctgaagaata atccaaccga tacctgaagc cgcatctttt cacgatgcgg
//
LOCUS       DAXHSJ010000200         2549 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000200 DAXHSJ010000000
VERSION     DAXHSJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.200"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            226..2451
                     /locus_tag="ACFM8U_001954"
     CDS             226..2451
                     /locus_tag="ACFM8U_001954"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC6335130.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPANPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQGGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDATVMSKKALLAFLAEQVQDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT          691 a          774 c          595 g          489 t
ORIGIN      
        1 atcaatttca ccgaaaatat gccgccgcac atcatgcaca gcacctccga caccgccgcc
       61 aaacgcggga aaaccatttc ccccttcctt tcacgccgcc gcgttcaaac acgccaagat
      121 cgaacaaagc gcgaacaaag ccgccaaaat atgccataat agccgcgcac attccccgcg
      181 caatgtgttc caaaagaaaa aaccaaccta caagagaata atcctatgac tcaaaaatcc
      241 accattgttt atacccatac cgacgaagcc cccgcgctgg cgacccaatc gctgttgccg
      301 attgtgcagg cgtttgcccg ccacgccgat attgatgtca aaactgccga catttccctg
      361 tccggccgta ttttggcggc attccccgaa tacctgaccg aagcgcagcg cgtacctgat
      421 gcgcttgccg aattgggcga actggtgaaa cagcccgatg caaacgtaat caaactgccg
      481 aacatcagcg catccgtacc acaactgact gctgcgatta aagaattgca gtctaaaggc
      541 tttgccgttc ccgactatcc cgccaaccct caaaccgatg aagaaaaagc cgtgcgcgaa
      601 cgctacgacc gcatcaaagg cagcgcggta aaccctgtcc tgcgtgaagg caactccgac
      661 cgccgcgcgc ctaaagcagt gaaaaacttt gccaaaaaaa atccgcacag catgggcgca
      721 tggacaaaag actccaaaac ccacgttgct accatgcaag gcggcgactt tttccataac
      781 gaacaatccg ttaccgtacc cgatgcgact tccgtatcca tcgtgttcac cgacaaacaa
      841 ggcaacaaaa aagagctgcg cgagcctgtt gccctgaaag ccggcgaaat catcgacgcg
      901 accgtaatga gcaaaaaagc cctgctcgcc ttccttgccg aacaagtgca agacgcaaag
      961 gcaaaaggcg tattgttctc gctgcacatg aaggccacca tgatgaaagt gtccgacccg
     1021 attatcttcg gacacgccgt caaagtgttc ttcgcgcctg tatttgaaaa attcggcggc
     1081 aaactggctg ccgccggtgt caacgtcaac aacggcttcg gcaacctgct tgccaatttg
     1141 gacaaactgg atgcggacac ccgcgctgcc gttgaagctg aaatcgccgc cgtttacgcc
     1201 gccaaccctg atttagccat ggttgattcc gacaaaggca tcaccaacct gcacgttcct
     1261 agcgatgtca tcgtcgatgc ttctatgcct gcgatgattc gtaattccgg ccgtatgtgg
     1321 gacaaaaacg gcaaagcaca agacaccaaa gccgtgattc ccgacagcag ctatgccggc
     1381 gtttaccaag caaccatcga cttctgccgc gaacacggcg catttgaccc gacaaccatg
     1441 ggtactgtgc ccaacgtcgg actgatggcg caagcggcgg aagaatacgg ctcgcacaat
     1501 aaaactttcg aaatcgaagc cgacggtcag gttcaagtca tcgatgcagc gggcaaagtc
     1561 ctgatgcagc acgacgttga agccggcgat atttggcgta tgtgccaaac caaagacgct
     1621 ccggttaaag actgggtaca actcgccgtc aaccgcgctc gtctgagcaa cacgcctgcc
     1681 gtgttctggc tcgacgaaaa ccgtccgcac gacaagagcc tgctcgccaa ggtcaaagcc
     1741 taccttgccg aactggatac caatggcctc gacatccgcg tcctcgctcc tgaagaagcc
     1801 gccaagttca gcttgggtcg tctgaaaaac ggcgaagaca ccatctccgt aaccggtaat
     1861 gtcttgcgcg actacctgac cgacttgttc ccaattttgg aactcggcac aagcgcgaaa
     1921 atgctgtcta tcgttccatt gatgaacggc ggcggtatgt ttgaaactgg cgcgggcggt
     1981 tctgcaccga aacatgttca acaattcctc gaagaaaacc atttgcgctg ggactctttg
     2041 ggcgaatttc tcgcgctcgc cgtatcgttt gaacatttgg cgcaaaaaac cggcaatgcc
     2101 aaagcccaag tcctcgccga cactttggat gcagccaccg aaaaactgct gttgaacgac
     2161 aaatcgccta aacgcaaagc gggcgaactc gacaaccgcg gcagccattt ctaccttacc
     2221 ctctactggg cgcaagaatt ggcggcgcaa gacaaagatg ccgaactgaa agccgcattt
     2281 acgccattgg cagccgcttt gaccgccgac gaagcgaaaa tcgttgccga gctttccgcc
     2341 gtacaaggca aagcggccga catcggcggc tactacgccg ccaatcctga aaaagccgca
     2401 caagtgatgc gtccgagcgt aacctttaat caggcactaa acgccttata agcacaaagg
     2461 taaaaatgcc gtctgaacat tcgtcgttca gacggcatta gttatcccta tctgcctgat
     2521 tatgattaaa cgccgcaccc attggatat
//
LOCUS       DAXHSJ010000201         2542 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000201 DAXHSJ010000000
VERSION     DAXHSJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.201"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            53..610
                     /locus_tag="ACFM8U_001955"
     CDS             53..610
                     /locus_tag="ACFM8U_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC6335131.1"
                     /translation="MGFGCREGREFCKGLSLPEIIRDLLEMPSETAIADITRTYSAHY
                     LNPNNRNMSLFPQALPCLDKLKAQGYWLAVATGKGRAGLDNAISQTATGGYWLATACA
                     GEYPSKPSPEMVFGICGKLGLDPKEALVVGDTAHDLHMAANAGAAAVGVATGAHSREQ
                     LLGAPHLAVLDDLSELPGFLAQHYA"
     gene            complement(697..1665)
                     /gene="ispH"
                     /locus_tag="ACFM8U_001956"
                     /gene_synonym="lytB"
     CDS             complement(697..1665)
                     /gene="ispH"
                     /locus_tag="ACFM8U_001956"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC6335132.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     QEVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1694..2221)
                     /gene="lspA"
                     /locus_tag="ACFM8U_001957"
     CDS             complement(1694..2221)
                     /gene="lspA"
                     /locus_tag="ACFM8U_001957"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC6335133.1"
                     /translation="MVLFYRLLKNMSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQ
                     YRERVNVIPSFFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVTVSAYLVRAILRDEF
                     AILGKTGAAMIIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLA
                     VLDNIVHRKTQEEKY"
BASE COUNT          586 a          752 c          623 g          581 t
ORIGIN      
        1 gtttgggtag aattaggagg tatttggggc gaaaacagcc gaaaatctgt gtttgggttt
       61 cggctgtcgg gagggaaggg aattttgcaa aggtctcagc ctgcccgaaa tcatccgcga
      121 cctgctcgaa atgccgtctg aaaccgccat tgccgacatc acacgcactt attccgcaca
      181 ttacctcaat cccaacaacc gcaatatgtc cttatttcct caggcactgc cctgtctgga
      241 caagctcaaa gcacaaggat actggcttgc cgtcgccacg ggcaaagggc gggcgggttt
      301 ggacaacgcc atcagccaaa ccgccaccgg cggctattgg ctcgccaccg cctgcgcggg
      361 ggaatatccc tccaaaccct cgcccgaaat ggtattcgga atctgcggca aactgggact
      421 cgatccgaaa gaggcattgg tcgtcggcga tacggcgcac gacctgcata tggcggcgaa
      481 cgcgggtgcg gcggcagtcg gcgttgccac cggcgcacat tcgcgcgaac agctccttgg
      541 cgcaccgcat ctcgccgtat tggacgactt gtccgaactg cccggttttc ttgcacagca
      601 ttacgcctga ttggtttccg catccggcac gcggcaaaaa tgccgtctga agcctgttca
      661 gacggcattt gtgttgccca aacattcaac gcttgctcaa cgcttgcaca aatcgggttt
      721 ggtttcgccc tcgcggcgca actctttggg caggacgaac acgatgcttt cttccgcacc
      781 ctcgccttcg cgcacggttt catgccccca cccgcgtatg gttgccaata cttcctgcac
      841 caacacttcg ggcgcggacg cgcctgccgt tacgccgact ttgtttttgc cctcaaacca
      901 tgcgcgttgc aggtagcctg cattatccac catatacgca tcgattccgc gcgatgccgc
      961 cacttcgcgc aagcggttgc tgttagacga attgggcgaa ccgaccacaa tcacgatgtc
     1021 gcactgttct gccaactctt tgacggcggt ttgccggttg gtcgtcgcat agcagatgtc
     1081 ttccttgtgc ggattgcgga tattggggaa acgcgcgttc agcgcggcga tgatgtcttt
     1141 ggtttcatcg accgagagcg tggtttggct gacataggcg agtttgtcgg ggtttctgac
     1201 ttcgagtttt gccacatctc ctaccgtttc gaccaaaagc attttgcccg gcgcaagctg
     1261 ccccatcgtt ccttcgacct cgacatgacc cttatgtccg atcatgatga tttcacagtc
     1321 ttgggcatcc agtcgggcga cttccttatg cactttcgtc accagcgggc aagtcgcatc
     1381 aaatacccgg aaaccacgtt ccgccgcttc ttgctgcacc gccttcgata cgccgtgtgc
     1441 cgaataaacc agcgtcgcgc ccggcggcac ttccgccaag tcttcgataa acaccgcgcc
     1501 tttttcacgc aggttatcga cgacgaattt gttgtggacg acttcgtggc gcacataaat
     1561 cggcgcgccg aattcttcca aagcacgttc gacaatactg attgcccgat ccacaccagc
     1621 gcagaagccg cgcggattgg caaggatgat ggttttctcg ttcataagcc cggtatttcg
     1681 ttttcagacg gcatcaatat ttttcttctt gggttttacg gtggacgatg ttgtccaaca
     1741 ctgccaacac cgcaccgacg cagataaagc tgtcggcaat attaaaggcg ggataaaacc
     1801 aattttgcca ataaaacaat aagaaatcga cgacatgacc gtgtatcagg cggtcgatga
     1861 cattgcctaa cgcaccgccg ataatcattg ccgcacccgt tttgccgagg attgcaaact
     1921 catcgcgcaa gatggcgcgt accaaatacg cgctcaccgt caccgccagc accaaaaaaa
     1981 agtatttttg ccagccgccc tgatcggcaa ggaagctgaa cgccgcaccc gggttgtaca
     2041 ccagcgtcaa atcgaaaaag gaaggaatga cattgacgcg ttcccgatac tgaaacgacg
     2101 acagcaccgc ccacttcgac cactggtcca gcacgatggc ggcaagtgcc aatacccaat
     2161 agcgcgtttt acttgaaaca gatgaagaca tatttttcaa cagccggtaa aagagtacca
     2221 ttttacccga aaaccccctt tcctgtaccc gaaacggcaa atgccgtaat cttaaaaccc
     2281 gtcattcccg acaacaccgt aatctcgaaa cccgtcattc ccgcgcaggc gggaatccag
     2341 acctgtccgc acagaaactt atcggataaa aacagttgcc caaaccccgc gttctatagt
     2401 ggattaaatt caaaccagta cggcattgcc tcgccttaac tcaaagagaa cgattctcta
     2461 aggtgctgaa gcaccgagtg aatcggttcc gtactatttg tactgtctgc ggcttcgttg
     2521 ccttgtcctg atttaaattt aa
//
LOCUS       DAXHSJ010000202         2527 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000202 DAXHSJ010000000
VERSION     DAXHSJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.202"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..357
                     /locus_tag="ACFM8U_001958"
     CDS             <1..357
                     /locus_tag="ACFM8U_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6335134.1"
                     /translation="TTMGERLLTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIK
                     KGENPAIITHWNNRVNQAEEDNYSTSVGSCINGFTVQYYPFIREKQQLTQQELVGYHQ
                     QVEQLVQSFVNNSSKK"
     gene            374..648
                     /locus_tag="ACFM8U_001959"
                     /pseudo
     CDS             374..648
                     /locus_tag="ACFM8U_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(735..1868)
                     /locus_tag="ACFM8U_001960"
     CDS             complement(735..1868)
                     /locus_tag="ACFM8U_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335135.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGKGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYYIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     IYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQIYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVRRYAEAAARRSGGRRDLSY"
     gene            complement(1988..2482)
                     /locus_tag="ACFM8U_001961"
     CDS             complement(1988..2482)
                     /locus_tag="ACFM8U_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335136.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEVENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQLVKNLFAEEVPENNIVCDISHFLHFADWA
                     EADIPNQSRLYAEYWWEMETFKISVLEDWERLGRPLDWKKWKDQYQSEAFELVKKLNR
                     SKQE"
BASE COUNT          706 a          603 c          478 g          740 t
ORIGIN      
        1 acgactatgg gagaaagatt gcttacttac ggggttaaaa aaatgccatc tgcctatcct
       61 gaatatgaag cttatgaaga taaaagacat attcctgaaa atccatattt tcatgaattt
      121 tactatatta aaaaaggaga aaatccggcg attattactc attggaataa tcgagtaaac
      181 caggctgaag aagataatta tagcactagc gtaggttcct gtattaacgg tttcacggta
      241 cagtattacc cgtttattcg ggaaaagcag cagctcacac agcaggagtt ggtaggttat
      301 caccaacaag tagagcaatt ggtacagagt tttgtaaaca attcaagtaa aaaataattt
      361 aaaggatctt attatgaatg agggtgaagt tgttttaaca ccagaacaaa tccaaacctt
      421 gcgtggttat gcttcccgtg gcgataccta tggcggttgg cgttatttgg ctaatttggg
      481 tgaccgttat gcggataatg ctgctgcaat tgtcggtaag gatgcaaact taaatggttt
      541 gaatttatgg atgaaaaaag ggtggaaaac ctatgggatg atacggtcgg taaaaagatc
      601 cgtttaatgt gtatttccgt tttttggatt gtggttttca atttatagcg aatcggattc
      661 ggcatatacg gcattgcaaa aagcgtctga ctctccaatg ccgtctgaaa accggtttca
      721 gacggcattt gcgttcagta agaaaggtcg cgcctgccgc ccgaacgtct cgccgcagcc
      781 tctgcataac ggcgcacctc tttttccaag tcttccaagt tcaaaggaaa atcaggcagt
      841 ctgtctccct gtttctcttc gcggataatc cgcccaccat ccaaatacca cgtctgttgc
      901 gcatgataga tctgcatatc cgccgttacg ccatccgctt tcaatgctac cgtcgaagat
      961 tgtgcaataa aaagatttcc gtttttcaaa taatattcga aactctggcg ttttttccca
     1021 ttgtcgaaac tccaatagat tttctgcggc agaccgtccg catcatagcc gaccacaaga
     1081 ctgttcgcct tcatcccccg gggcatcaat tcccgcatat tctgataaaa cacataatcg
     1141 cgcgagtccg acgcaatccg gttgctctct tcgcggaagt cccaaacctt ctgctcatca
     1201 ttcgcgacat cccggtattt cgccaaatat acctgggcca tctgataaca cccgaggcaa
     1261 tgctcataaa catcttcccc gattttcccg cgccccgacg catcaaatac cgaaccgtcc
     1321 ggttgccaaa caacccgata ttctcctgtc gtttcataat tttctccgtg agccgttccg
     1381 ccgtacacat ttacagaaaa cggacgatcg tttcgataca gatattcggc attaacaaac
     1441 gcttccggcg agcgttgcga aagcgaaacc gcaaccaaac caccctcgcc gatatagtaa
     1501 tccagccaaa cctctttccc atgttcctgc tccgttacgt gaaaccattt cgccttttct
     1561 ttcaaacgac tgagccggat agcgagcgcg agataatcct tctccgactg caacggaccg
     1621 tcatccacag tttcggcaag actttcctcc gtcctcatcg attccttcac gatgacgacc
     1681 gccctgtcgg catttcggaa caggcgggca agtttcgcca caaaagcatt cggattttca
     1741 ggtatttcag ttgccgtatc gctcaaaaac caacgcggat taatctcata ggcaataccc
     1801 ttccccagcc aaaaggcaaa tacaagtgca aaaaatgaca acaataccgg tttgaatttt
     1861 ttaaacatat ttattttccg tttaacagga tatatcgatt atatcagacg agctttaatt
     1921 gtttgatttt taatctaatc tacggttgct accgtacttt aaaaacccct atgcaggctg
     1981 cggcttgtta ctcttgtttg cttcggttca attttttgac taattcaaat gcttcagatt
     2041 gatattgatc tttccatttt ttccaatcta acggccttcc caagcgttcc caatcttcta
     2101 aaaccgatat tttgaacgtt tccatttccc accaatattc ggcatataaa cgtgattgat
     2161 tgggaatgtc ggcttctgcc caatcggcaa aatgcaaaaa atgagaaata tcgcaaacta
     2221 tattattttc aggaacttct tcggcaaata gattcttgac caactgtaac gcttcggtct
     2281 gatatttttc tgtccatttc tcccattttt caggctttcc cgatatttcc caatcttcca
     2341 atgcggaggc atttaagatt tccaattccc accagcagtc gtaataacgg gaggattggt
     2401 tgggaatttt attttctact tcggaaacaa attgggtaaa actaaaaata tccttaacga
     2461 ttttttcaaa tctattttcc ataaattgct gcctcttcca aattatagtg gattaaattt
     2521 aaatcag
//
LOCUS       DAXHSJ010000203         2524 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000203 DAXHSJ010000000
VERSION     DAXHSJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.203"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(42..848)
                     /locus_tag="ACFM8U_001962"
     CDS             complement(42..848)
                     /locus_tag="ACFM8U_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC6335137.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1068..2438
                     /gene="ffh"
                     /locus_tag="ACFM8U_001963"
     CDS             1068..2438
                     /gene="ffh"
                     /locus_tag="ACFM8U_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC6335138.1"
                     /translation="MLDNLTGRFSNVFKNIRRQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          693 a          635 c          662 g          534 t
ORIGIN      
        1 gtctgaaact cggggactca gacggcatct gttggctctt cttatctttt cagaatgatt
       61 tccaatacga acttgctgcc catataggca atcataaggc tgacaaatcc gatgatggtc
      121 cacacggcgg cttttttgcc gcgccatgcg gtcatgctgt gcttgagcag cagtccgccg
      181 taaatcagcc atgacaatat gccgaatacg gttttatggg taaaggtcat gggtttgccg
      241 aatacggctt cggcgaagaa tgtgccgctg acgacggaat aggtcagcag gatgaaacct
      301 gcccacatgg cctggaacat gagtttttcc aaactgagca gcgacggcag gaatcctgcg
      361 agcttggaga agctcctgcg gtgcaggctc cgattcagca gcagggtcaa aacggacaat
      421 aatgttgcga tgccgaacag cccgtatgca agcagtgatg tgccgatatg cagcataaag
      481 ggaaggtcgg taatttcata tcccgagaat tttccgggaa aaaccaaacc tgacagcagc
      541 atcagtgcgg cgcagggata cagcagcaac tgcactccgc gcagcggata aaagaagctg
      601 ccggcaaaat aaataaacag catcatccaa acaatcaggc tgccggaata cccgaagccc
      661 ataatgatga ttttgtcttg aatgaccggc atcagcagtg ccgcgccgtg gacggtcaat
      721 gccgcgccca aaaccggcaa ttccgtcttc cacgggtaat cccgaccgcg cccctgctgt
      781 tggcagtgcc atgcaaatgc acccaatcct gcgtaaaccg ccgtcaaaaa gatgaaaact
      841 gtcggcatgg tggactttct ctatctatac tgttgcgccg tctgcggccg cttatgaaat
      901 attggaactt ttaacgttgg aattgtaaaa tccccatttc gggcaagcct tgacggattt
      961 gccgatatgc tgtccggcac acaagccgca tcaaattatt ttgattttat tttaacaaag
     1021 aatgcccctg atggggcaag ctattcttat tcagaccaag gaccagtatg ttagacaatt
     1081 taaccggccg cttcagcaat gtcttcaaaa acatccggag gcaggccaaa ctgaccgaag
     1141 acaatattaa agaagccttg cgcgaagtcc gcctcgccct gcttgaggcg gatgtcgccc
     1201 tgcctgtcgt caaagagttc gtcaacaacg tcaaagaaaa ggccctcggt caggaagtgg
     1261 tgggcagcct gacgccggat caggcattta tcggcgtggt caacaaagcc ctgaccgagc
     1321 tgatgggcag ggaaaacaaa acgctggatt tgtcggttgc gccgcccgcc gtcgtgttga
     1381 tggcaggttt gcagggcgcg ggcaagacga cgaccgccgg caaactcgcc cgcctgctga
     1441 aaaacgatca gaagaaaaag gttttggtcg tatctgccga cgtttaccgc cctgccgcga
     1501 ttgaacaact gcgtctgttg gccgaacagg tcggcgtgga ctttttccca tccgatacca
     1561 accaaaaacc ggttgagatt gcaactgccg ccgtcgatta cgccaaaaaa catttttacg
     1621 atgtattgat ggtcgatacc gccggccgtt tggcaatcga tgaggagatg atgaacgaaa
     1681 tcaaagccct tcacgcggcg gttaacccgg tggaaacttt gttcgtcatc gatgcgatgc
     1741 tgggtcagga tgcggtgaac actgctcagg catttaatga agccctgccg ctgaccggtg
     1801 tcgtattgac caagatggac ggcgattcgc gcggcggcgc agctttgtcc gtgcgccacg
     1861 taaccggcaa gccgattaaa tttatcggtg tcggcgaaaa aatcaacggc ctcgaacctt
     1921 tccaccccga ccgtcttgcc agccgtatcc tcggcatggg cgacgtattg accctgattg
     1981 aagacgtcca aaaaggtatt gatgaagaag ccgccgccaa aatggcgaaa aagctacaaa
     2041 aaggcaaagg cttcgacctc aacgacttta aagaacaaat ccagcaaatg cgcaatatgg
     2101 gcggtttgga aaacctgatg tcgaaaatgc cgggcgaact gggtcaaata tcgaaacaaa
     2161 tccccgaagg aacggctgaa aaagcgatgg gcaaagtgga agccatcatc aactcgatga
     2221 ctccaaaaga acgcgccaac cctgccctgc tcaaagccag ccgcaaacgc cgtattgcaa
     2281 tgggtgcggg cacgaccgta caggaagtga acaaattgct caaacagttt gaacaaatgc
     2341 aacaaatgat gaagatgttc agcggcaacg gcttgggcaa actgatgcgt atggcgaaag
     2401 gaatgaaggg aatgaaagga atgttcccgg gtttgtaagc cgatttgacc gaaaacgccg
     2461 tctaaaattt cagacggcgt ttttgtttta tattctgatt tatagtggat taacaaaaat
     2521 cagg
//
LOCUS       DAXHSJ010000204         2523 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000204 DAXHSJ010000000
VERSION     DAXHSJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.204"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(227..1861)
                     /gene="groL"
                     /locus_tag="ACFM8U_001964"
     CDS             complement(227..1861)
                     /gene="groL"
                     /locus_tag="ACFM8U_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC6335139.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGMGGMM"
     gene            complement(1954..2244)
                     /locus_tag="ACFM8U_001965"
     CDS             complement(1954..2244)
                     /locus_tag="ACFM8U_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC6335140.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            2455..>2523
                     /locus_tag="ACFM8U_001966"
     CDS             2455..>2523
                     /locus_tag="ACFM8U_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6335141.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAP"
BASE COUNT          526 a          658 c          658 g          681 t
ORIGIN      
        1 ttcgcgggaa tgacggcgga aaggttgctg tttttccgat aaattcctgc cgctcttcgt
       61 ttttgggatg gcgggaaata aaacaaaagc gcgcgtatca aaaaataaca atgcaaagaa
      121 cggtgttggc aattttaagg tttgcgcctt agccgcactt attcgcaaaa aaaccgcacg
      181 gcgttgaccg tgcggcttgt tgtctgaagg cttcagacgg cattgcttac atcatgccgc
      241 ccataccacc catgccgccc atatcaggca cagccggttt gtcttcgggg atttcagcga
      301 tcatgcaatc agtggtcagc atcaagccgg cgatagatgc ggcgtgttgc agcgcggaac
      361 gggttacttt ggcggggtcg agtacgccca tttcgatcat gtcgccgtat tcgccgctgc
      421 cagcgttgta accatagttg cctttgcctt ccaacacttt gttcacaacc acgctgggtt
      481 cgccgcctgc gttggcaacg atttggcgca gcggagactc aacggcgcgc aagacgattt
      541 gtacgcctgc gtcttggtcg gcattgccgg tgtgcaggtt ttccaaagca gcacgggcac
      601 gcaacagggc tacgccgccg cctgcaacca cgccttcttc aacggctgcg cgggtagcgt
      661 gcagcgcgtc ttccacgcgg tcttttttct ctttcatttc gacttcggtc gcggcaccga
      721 ctttgatgac tgccacgccg cctgccaatt tagccacgcg ctcttgcagt ttttctttgt
      781 cgtaatcgct ggttgcggtt tcgatttgtt ggcggatttc ggcaacacgc gcttcgattt
      841 gggctgcgtc gccaaagccg tcgatgatgg tggtgttttc tttaccgatt tcgatgcgtt
      901 tggcttgacc caagtcgtcc aaagtcgctt tttccaaaga cagaccgact tcttcggaaa
      961 tcaccacgcc gccggtcagg atggcgatgt cttgcaacat ggctttgcgg cggtcgccga
     1021 agccaggggc tttcacggca acggttttca ggatgccgcg gatgttgttc accaccaaag
     1081 tcgccaaggc ttcgccttct acgtcttcag cgataatcaa cagcggacgg ctggctttgg
     1141 ccacttgttc caaaacaggc aacaggtcgc ggatgttgct gatttttttg tcgaacaaca
     1201 atacaaacgg attgtccaaa gcagcgattt gtttttcagc gtcgttgatg aagtaaggag
     1261 acaggtagcc gcggtcgaac tgcatacctt caaccacatc cagctcgttt tccaaagatt
     1321 tgccgtcttc aacggtaatc acgccttctt tgccgacttt ttccatcgct tcggcgataa
     1381 tcgcgccgac ttgttcgtca gagttggcgg aaatcgagcc gacttgggcg atttctttag
     1441 aagtgtcgca aggtttggcg atgtttttca gctcgtcaac caaagcggca acggctttgt
     1501 cgataccgcg tttcaggtcg gtcgggttca taccggcggt aacgtatttc ataccttcgg
     1561 caacgatgga ttgcgccaat acggtggcgg tagtcgtacc gtcgcctgcc acgtcgttgg
     1621 ttttggacgc gacttctttt accatttgcg cgcccatatt ttcaaacttg tctttcagtt
     1681 cgatttcttt ggcgacggtt acgccgtctt tggtgatgtg cgggccgccg aatgcgcggt
     1741 caaccaccac gttgcggcct ttggggccca aggttacgcg cacggcgttt gccagaatgt
     1801 tcacgccgtt taccattttt tggcggactt cattgccgaa ctgtacgtct tttgctgcca
     1861 tttcaattct ccaaaaatca ttaaaactgt ctgataaaac cgtttatgcc gtctgaaggc
     1921 ggtttgccgt ttcagacggc atcgtgtccg tatttatttt tcaacgatgc cgaaaatatc
     1981 ttcttcacgc attaccaaca gctcttcgcc gtcggctttg acggtttggc cgctgtattt
     2041 gccgaaaatg attttgtcgc cgactttgac atccagcgga cggcgcgcgc cgtctttgcc
     2101 gattttgccc gcgcccacgg cgatgacttc gcccatatcg ggtttttcgg cggccgcgcc
     2161 cggcaaaacg atgcccgatg cggttttttc ttcagcttcc aagcgtttga cgacaacgcg
     2221 gtcgtgtaaa ggacggatgg tcatatttat gctccgataa atgttttgaa aacaatcatc
     2281 tgcccgaacg gttcggacag attgaagtgg aaaccggaca gccgtcaagc agctgcccgt
     2341 ataagtcggc aaaattaggg tgtgtgcggg taaattcaag tgaggcggaa aaaatttatt
     2401 tccgccgttt tttatagtgg actaaattta aggggctgta ctagattagc agatatgtta
     2461 ccctcgaaat atgaagataa cgcactgcaa attaaagaaa aaagtacaga aagaactgct
     2521 ccg
//
LOCUS       DAXHSJ010000205         2514 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000205 DAXHSJ010000000
VERSION     DAXHSJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.205"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(8..133)
                     /locus_tag="ACFM8U_001967"
                     /pseudo
     CDS             complement(8..133)
                     /locus_tag="ACFM8U_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            complement(463..873)
                     /locus_tag="ACFM8U_001968"
     CDS             complement(463..873)
                     /locus_tag="ACFM8U_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335142.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(1461..1844)
                     /locus_tag="ACFM8U_001969"
     CDS             complement(1461..1844)
                     /locus_tag="ACFM8U_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335143.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(1865..2071)
                     /locus_tag="ACFM8U_001970"
     CDS             complement(1865..2071)
                     /locus_tag="ACFM8U_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC6335144.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(2037..>2514)
                     /gene="mafB"
                     /locus_tag="ACFM8U_001971"
     CDS             complement(2037..>2514)
                     /gene="mafB"
                     /locus_tag="ACFM8U_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6335145.1"
                     /translation="PTARPMQTLDGEMAGGNKPPKPSTQQHPTHSDNNIGLPASYVKP
                     DTSISPTGTIQDRIRWTKSKFPTEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQT
                     SDKILGYQTEHRRVRYDINNNIYVLANPKTFKIKTMFKPNLGKKYYDGEFKKDMGN"
BASE COUNT          720 a          461 c          410 g          923 t
ORIGIN      
        1 gtgtgaatca aggttgccct tgtctatgtt gtcgcactac ttcatcgctg tatccatacc
       61 cctcatgact gttatcagcc tctacggatg gggcggagtt acccgtatga ttgccgtatg
      121 gtcggatact tgtatccgac aaaaatattt gacgcatcta tcgtttccga aatgccgctg
      181 ttggaaaatg tcggattcga gaatccgacc tacggttgct gaacaatgtt ttgtagaact
      241 tgctcaaaat ccatgtttcg ccattttcct ttatatatac ctaatacagc acgggttacg
      301 atttcgtttt aagtcataaa tagccattta cgtcctctgc taattttcag cgagtagatt
      361 gggttaccaa acccaacaaa aaagcagcct gaaaaatttc aggatgcttg ttttatgttt
      421 tttatcaatg aattaggttt taatccagcc tgcgcttgct agttaaacag tatttacata
      481 aaaatcttgt actgaacaaa caagcgtatc atgttcatca tgaaatgatg ggcataacgt
      541 taatctacaa atgtttacat ttaaagatat tttttcagtt tcaatatttt taatttgtaa
      601 ggttaataca tgaggatatt tgtgatatag ctgatttttt aattgttcgt tgtctaatat
      661 agcaaaaggt aattttgcac acaaataacc aataactttg tttttatgaa aaaaaactat
      721 ccaattttca attccttcat cccaatcaaa agtaaaatcc gttccttcta tgataaattt
      781 agcacaagat attaaatcta acttaaccca agtggattta tacagactca atgctgatga
      841 agttataata ttatctaaat tataaatttt cataaatcaa cgttccttac ctggtgttat
      901 atgcacaggt ctaatcccat ctttattcgg ctttctacga taaatgttaa ttatatgtgt
      961 cggttttccg tctgtatagc caactacgtc gttcgttgga acttttgcct tattttgact
     1021 atcccataaa ttatgtttat ctgcatatct agcagcatct aatgtagctt tttccgcatc
     1081 aacatctgat ttaaataaac ttttgctatc tatcttgtct agactagatg catcaggttt
     1141 gtgtcgtaaa aagcactgtc ctgtaatagg ctcgcggcag caagttgtgc ttccggactt
     1201 aattttccta aatcattaat accttgtaaa gtttgctgcg cagagatgta ggtcggatat
     1261 ttgtatccga caaaaatatt tgacgcatct atcgtttccg aaatgccgct gttggaaaat
     1321 gtcggattcg agaatctgac ctacggctgc tgactatttt cttgtttata taattctaca
     1381 actgcaaact actatcaaat ctttttatct ctggctctac actcagcaag aatattttta
     1441 ttttacaaaa cataggctag ttatttgagt atattgtcaa attctaattt gtttttttga
     1501 tagatttttt ttataccatt aatacattca ttagttgtaa ttctgccaag cgaatgtaat
     1561 ttatcaatat caaaacaatc ggtataaacc ctattcacag ttttatcttg tatttcctta
     1621 tgaatactta atatattgag atagtttaga aattttattt tctcagattg aggtaactca
     1681 tcaaaaccaa gccaataata aaaattgtta tatgatttta aaaaatcaac aatttttatt
     1741 tttcctaaaa ccaaatcttc gactaactgg gttgcataaa taatatcatt tatgatttta
     1801 acaggtaaat tatttgatag gtaaccatgg gattccgtca tcattgattt ctctaggtgc
     1861 ttatttatct gattggtaag ccttttgcgt aagcttcttt cgcctccgca agtgtcattt
     1921 cattagggcc accatctata ttagtttctc ctgtattttg ccactgacaa acgtcacaga
     1981 tatcatagtc cataacttca gttccgcaaa caggacaatg tagccatatt tctccgtcaa
     2041 tttcccatgt cttttttgaa ttctccatca taatacttct ttcctaagtt tggtttaaac
     2101 attgttttga ttttgaatgt ttttggattg gccaaaacat agatattgtt attgatatca
     2161 tagcgcactc gtctatgttc cgtctgataa cctaatatct tgtccgatgt ctgttttgat
     2221 aacaaatcag acgccatttt ttggtagtct tcaatggtta tatcgccaaa ttcttttcca
     2281 tgagctttga aatgtccatt taaagatttc tcagtaggaa acttggactt tgtccatctg
     2341 atgcggtctt gaattgttcc tgtcggagaa atagatgtat caggtttaac atatgaggca
     2401 ggtaagccga tattgttatc agagtgtgta gggtgttgct gcgtacttgg ttttggtggc
     2461 ttattcccac ctgccatctc cccatctaaa gtctgcatag ggcgggcagt aggt
//
LOCUS       DAXHSJ010000206         2446 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000206 DAXHSJ010000000
VERSION     DAXHSJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.206"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            36..1391
                     /locus_tag="ACFM8U_001972"
     CDS             36..1391
                     /locus_tag="ACFM8U_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HFC6335146.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIHNQYALILSYTLIGIAWAGIITYPLTIVTNALSGEHMGT
                     YLGLFNGSICMPQIVASLLSFILFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1395..2414
                     /locus_tag="ACFM8U_001973"
     CDS             1395..2414
                     /locus_tag="ACFM8U_001973"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFC6335147.1"
                     /translation="MSDTPTTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQ
                     EFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEAN
                     EGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDR
                     LTVSYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDL
                     EVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDR
                     NGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDIREATIAYG
                     IESLS"
BASE COUNT          452 a          669 c          729 g          596 t
ORIGIN      
        1 actatacttt tcaaatcaaa gaaaggattt accttatgtc ggaatatacg cctcaaacag
       61 caaaacaagg tttgcccgcg ctggcaaaaa gcacgatttg gatgctcagt ttcggctttc
      121 tcggcgttca gacggccttt accctgcaaa gctcgcaaat gagccgcatt tttcaaacgc
      181 taggcgcaga cccgcacagc ctcggctggt tctttatcct gccgccgctg gcggggatgc
      241 tggtgcagcc gattgtcggc cattactccg accgcacttg gaagccgcgt ttgggcggcc
      301 gccgtctgcc gtatctgctt tatggcacgt tgattgcggt tattgtgatg attttgatgc
      361 cgaactcggg cagcttcggt ttcggctatg cgtcgctggc ggctttgtcg ttcggcgcgc
      421 tgatgattgc gctgttagac gtgtcgtcaa atatggcgat gcagccgttt aagatgatgg
      481 tcggcgacat ggtcaacgag gagcagaaag gctacgccta cgggattcaa agtttcttag
      541 cgaatacggg cgcggtcgtg gcggcgattc tgccgtttgt gtttgcgtat atcggtttgg
      601 cgaacaccgc cgagaaaggc gttgtgccgc agaccgtggt cgtggcgttt tatgtgggtg
      661 cggcgttgct ggtgattacc agcgcgttca cgattttcaa agtgaaggaa tacgatccgg
      721 aaacctacgc ccgttaccac ggcatcgatg tcgccgcgaa tcaggaaaaa gccaactgga
      781 tcgaactctt gaaaaccgcg cctaaggcgt tttggacggt tactttggtg caattcttct
      841 gctggttcgc cttccaatat atgtggactt actcggcagg cgcgattgcg gaaaacgtct
      901 ggcacaccac cgatgcgtct tccgtaggtt atcaggaggc gggtaactgg tacggcgttt
      961 tggcggcggt gcagtcggtt gcggcggtga tttgttcgtt tgtattggcg aaagtgccga
     1021 ataaatacca taaggcgggt tatttcggct gtttggcttt gggcgcgctc ggctttttct
     1081 ccgttttctt catccacaat caatacgcat taatcctgtc ttatacctta atcggcatcg
     1141 cgtgggcagg cattattacc tatcctttga cgattgtaac caatgccctc tccggcgaac
     1201 acatgggtac ttatttgggc ctgtttaacg gctctatctg tatgccgcaa atcgtcgctt
     1261 cgctgttgag cttcatattg ttcccgatgc tgggcggctt gcaggccact atgttcttgg
     1321 tagggggcgt cgtcctgctg ctgggcgcgt tttccgtgtt cctgattaaa gaaacacacg
     1381 gcggggtttg agcgatgagc gataccccca ctacccccgc tacccgcgat ttcggcctga
     1441 tcgacgggcg tgccgtaacc ggctatgtgc tgtccaatcg gcgtggtacg cgcgtctgcg
     1501 tgctggactt gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa
     1561 acctcgtggt gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca
     1621 aacagatagg gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga
     1681 cttaccgcgt ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg
     1741 ccgttacccg tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc
     1801 tgcaacagtc ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg
     1861 acgaggacga ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg
     1921 acccgacgct gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata
     1981 ttccgcaggg cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag
     2041 acgacctcga agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc
     2101 tgcgccgcga aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag
     2161 gccgtcccgc cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc
     2221 gcaacggctt ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg
     2281 tttacgacgc gctggcgacc gaggcgcaga cgctgcccga cagcctgcat tggcccgagt
     2341 tcggcaatat tcgtctgaac aagggtgata tcagggaggc gacgattgct tacggcatcg
     2401 aatccctttc ttaggagctt cctaacaccg gttgcagacg accttt
//
LOCUS       DAXHSJ010000207         2402 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000207 DAXHSJ010000000
VERSION     DAXHSJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.207"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..336
                     /locus_tag="ACFM8U_001974"
     CDS             <1..336
                     /locus_tag="ACFM8U_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC6335148.1"
                     /translation="LYIIATTVPAQQTFLDAAELMQWSIETEGLGLNVISHKILGKDH
                     AQVEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGSGKKFKAC
                     CGKYLKPVA"
     gene            complement(555..1349)
                     /locus_tag="ACFM8U_001975"
     CDS             complement(555..1349)
                     /locus_tag="ACFM8U_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6335149.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSCRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1453..2178
                     /locus_tag="ACFM8U_001976"
     CDS             1453..2178
                     /locus_tag="ACFM8U_001976"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC6335150.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFNGKG
                     KAHAAQLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGERMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          652 a          760 c          525 g          465 t
ORIGIN      
        1 ctatacatca tcgctactac cgttccggcg caacaaacat tcctcgatgc cgccgaactg
       61 atgcaatgga gtatagaaac cgaagggctg ggcttgaacg tcatctcgca caagatactc
      121 ggcaaagacc acgcccaagt cgaatttgaa gcctacttcc gagacggaca acaccgatcc
      181 gcgcatcacg aactgtccgg cttcgtcaac atcggcggac aatggtattt tatcgatccc
      241 accgttccgc atcctgcgat gaaacaaccc tgcatttgcg gatcgggcaa aaaattcaaa
      301 gcctgctgcg gcaaatatct gaaacctgtc gcataaaaat gccgtctgaa cgttcagacg
      361 gcattttcaa cgtgcaaaaa aaaaccattc ataccaaggg taagtatgaa tggtcaatac
      421 attgcgggaa aacgtcttac ttgctgcact gccgaaaagg gagaaacggc agcggtaatc
      481 agcggaaatg attgtacccg aattaatgtt aagaaacgtt aatcgcgaaa atatattaac
      541 aaacctgttg aaacctattg gttttcccgt atccacccga cccagcgttc aaacagcttc
      601 ggttcgagcg cggcaacgac cgagcgtttg aacacgtgtt caccactcca aaacccgtcg
      661 ccttccaaag tcgtcagcct gccgcccgcc tcctcgaaaa tcaacgcgcc ggcggcataa
      721 tcccacagct tctgcccacc gtgaacataa acatcataac gcccgcacgc cagataacac
      781 caatccaacg tactgctgcc catactccgt atcgttccaa aaggcgcgag cgtactcata
      841 cggctggaaa gtttgcccga acgcaaatat ttgatttcca cgcccgcaat cgcctcattg
      901 agttttttat ccacgagacg caaaggcagg cgggttccat taagaaacgc accctgcccc
      961 cgttcggcat aaaaacattc gccgctgacc ggattgtaaa tcacacccaa ttcggcgcgc
     1021 ccgttgcgga caaacgccac cgacacggca aaatgcggca gcccgttgac aaaattgttc
     1081 gtcccgtcta tcggatcgac aatccacagc cccttttccc ccgaatattg ttcccacaaa
     1141 gccgactgtt cctgccgcga catttcctcg cccaacatcg gactgtcgat taaaagcggc
     1201 aacgcggcgg caaaagccgt ctgcgcggca atgtccgcct cgctcaacat cgaaccgtct
     1261 tccttgcggc aagacggcgt attcaaaaaa cgcggcataa tttcggtttg cgcgatatgg
     1321 cgcacgactt tctgcaaacg gtgcaacact tcctactgtc ctcatatttt gaacttgcgg
     1381 cgcgcaaacg tataatgtcc gtttccatca cgccgctgcg acggattata accgtccgaa
     1441 ccgccaaaaa ctatgccccg attctacctg cccgaaaacc tttccgtcgg acaaaccgtc
     1501 gccctgcccg acaacatcgt ccgccacctc aacgtcctgc gcgtccgccc caacgaaaac
     1561 atcaccctct tcaacggcaa aggcaaggca cacgccgcac agctgaccgt tttggaaaaa
     1621 cgccgcgccg aagccgaaat cctgcacgaa gacacaaccg acaacgagtc cccgctcaac
     1681 atcacgctca tccaatccat atcttccggc gaacgtatgg actttaccct gcaaaaaagc
     1741 gtcgaactcg gcgtaaccgc catacagccc gtcatcagcg aacgctgcat cgtccgcctc
     1801 gacggagaac gcgccgccaa acgcctcgca cgctggcagg aaatcgtcat ctccgcgtgc
     1861 gaacaaagcg gcaggaacac cgttcccccc gtgctgccca tcatcggcta tcgtgaagca
     1921 ctcgacaaaa tgccgtctga aagcaccaag ctgattatga gcatcaaccg cgcccgcaaa
     1981 ctcggcgaca tccgccaccc gtccggcgca atcgtcttta tggtcgggcc cgaaggcggc
     2041 tggacagaac aggaagaaca acaggcattt gaagccggct ttcaggcggt tacactcggc
     2101 aaacggattt tacgcacaga aaccgcccca ctcgccgccc tcgccgccat gcagacgctt
     2161 tggggcgatt tcgcataaac agaaatgccg tctgaaaccc gttcagacgg cattttgcag
     2221 ccgattaaga tagtaggttc aaataagatt tcccccgccg tcattcccgc gaaagcaaga
     2281 atctagaaac gaaaaactac agggatttat ccgaaacaac aaaccctctc cgccgtcatt
     2341 cccgcgcagg cgggaatcta gaaatttaac gttgcggtga tttatcggaa atgactgaaa
     2401 ct
//
LOCUS       DAXHSJ010000208         2381 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000208 DAXHSJ010000000
VERSION     DAXHSJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.208"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(206..2338)
                     /locus_tag="ACFM8U_001977"
     CDS             complement(206..2338)
                     /locus_tag="ACFM8U_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFC6335151.1"
                     /translation="MTAVPTPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGEGEHAQFLIQ
                     DLVTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
BASE COUNT          652 a          722 c          534 g          473 t
ORIGIN      
        1 cttggtggga atttagggga ttttggggga ttttgcaaag gtctctagat gagtgaaaaa
       61 gaagtgcagg ctgcctaaaa agacagaaaa agtctttccg gcagcctgca ctttggtttc
      121 atttcagtca gtaaacccag taaacgacgg tctgaaaacg cagaacgtta cgaaaaaagc
      181 agcctacacg cccatccccc gccttctacc cgttctgtaa atcatacaga tagcggtaat
      241 atccgttcgg cttcgccagc aattcctgct gtgttcccgc ttccacaatc ctgcctttat
      301 ccatggcaat gattcggtgt gccgttttaa cggtggacag acggtgggcg ataatcagca
      361 ccgtccggtt ggcgcaaatg gcctgcatgt tctgcataat cgctcgttca ctttcataat
      421 ccagcgcgct ggtggcttca tcaaaaatca gaatgcgcgg attggtgatt aacgcgcggg
      481 caatcgcaat acgctgccgc tgtccgcccg acaagccggc cccttgttcg cccaccacgg
      541 tgccgtagcc ttccggcagc tccataataa actcgtgtgc gcccgccagt ttggctgctt
      601 cgataatgcg ttccagcggc atacccgtat ccgtcagcgc gatattgtcg cgtatgctgc
      661 ggttgagcag cacattctcc tgcaagacca cgccgacctg ccgccgcagc caggcaggag
      721 cggccaaagc caaatcgttg ccgtccacca acacccgtcc ctgctccggt acatacagac
      781 gctgcaccaa tttggtgagt gtggatttgc ccgaccccga acgtcccaca atccccagca
      841 cttcccccgc ccgaatccgc aggttcaaat cctgcaaaat cagcctgccg tccgccttat
      901 agcggaaatc gacatgttcg aacgtaatct ccccccggat atcgggcaaa gccaaatgcg
      961 aagacgcatt ctcggtcggc gcattcagaa tatcccccaa acgcgccacc gaaatcccca
     1021 cctgctggaa atcctgccac aactgcgcca aacggataac aggcgccgcc acctgtcccg
     1081 agagcatatt aaacgcaatc agctgcccca ccgtcagctt gccctcaatt accagccgtg
     1141 cgccaatcca caacgtcgcc accgtcacca gcttctgaat cagctgcacc ccctgctggc
     1201 cgaccaccgc caacttcgtt acccgaaatc ccgaagccac ataagccgcc aactgattgt
     1261 cccaacgctg cgtcatctgc ggctccaccg ccatcgcctt taccgtaccc accgcagtga
     1321 tgctttctac taaaaacgac tggttgtctg cattgcgcgc gaacttatcg ttcagacgcg
     1381 tccgcagtat cggactgata aatgccgacc aaaacgcata ggcaggcaac gaagccaata
     1441 ccacccaagt cagagtggag ctgtaatacc acatcaccgc cagaaagata aacgaaaacg
     1501 ccaaatccaa caccgaagtc agcgcctgac cggtcaagaa attgcgaatc tgctccaatt
     1561 cccgcacccg agccaccgta tcacccactc gtctgtgctc gaaataggat aaaggcaggg
     1621 aaagcagatg ccggaacaaa cgcgcgccca attccacatc aatacgtgaa gtcgtatgtg
     1681 caaacagata cgtccgcaaa ccgcccaaca caatctcaaa cagcgacacc accaacaaag
     1741 ccaccgacac cacatccaaa gtagagaatc cccgatgtac cagcaccttg tccatcacca
     1801 cttggaaaaa cagaggcgta atcagcgcaa acagctgcaa caccaccgac accaccaata
     1861 cttcaaaaaa caaccggcgg tatttgatta ccgccggaat aaaccaggta aagtcaaact
     1921 ttgccaaact gcccaatacc gaagcgcggg aagcaaccaa tatcagtttg cccgaatatc
     1981 tgttagaaaa ttcggcaaaa gacaataccg cagacttatt cgtaaccaaa tcctgtatca
     2041 aaaattgggc atgctcaccc tcaccgtctg ttttggccaa aatgaaatgg ttgccgtcat
     2101 cacaccatac caatgcgggt aaagtcgcca tagccaaacg tttaataggc tggcggacta
     2161 cctttgcctt caatcccaaa gatttggcgg ctaacagcca ttgcgtttca tttaaatcgc
     2221 tctgtgcgga agtacaaaat tcatgctgta tatcggcagg attggcggca atgccgtggt
     2281 aatgggcgag gatgatgagg gcggaaaggg cggggagagg tgtgggtacg gcggtcatga
     2341 tattgagatt gaataacggg taacaaaaaa tataccaaaa a
//
LOCUS       DAXHSJ010000209         2344 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000209 DAXHSJ010000000
VERSION     DAXHSJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.209"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            45..2258
                     /locus_tag="ACFM8U_001978"
     CDS             45..2258
                     /locus_tag="ACFM8U_001978"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6335152.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRGELKKYNVHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAISKIRAYIRQQNAD
                     TVREEGRVQIDKQLAKLTPKPNLQELAENLGFKKLDDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEQAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          597 a          760 c          569 g          418 t
ORIGIN      
        1 gctgttacaa taaactcgtt tttatcggaa cggaagaccc catcatgacc gccatcagcc
       61 cgattcaaga cacgcaaagc gcgactctgc aagaattgcg cgaatggttc gacagctact
      121 gcgccgcgct gccgaacaac gataaaaaac ttgtcttagc cgcccgttcg ctggcggaag
      181 cacattaccc cgccgatgcc gccacgccgt atggcgagcc gctgcccgac cacttcctcg
      241 gcgcggcgca aatggttcat gaactcgacc tgctccccga tgccgtcgcc gccaccctgc
      301 ttgccgacat cggacgctac gtccccgatt ggaacctatt ggtttccgaa cgctgcaaca
      361 gtaccgtcgc cgagctggtc aaaggtgtgg acgaagtgca gaaactcacc cacttcgccc
      421 gggtggacag cctcgccacg ccagaagaac gcgcccagca agcggaaacc atgcggaaaa
      481 tgctgctggc gatggttacc gacatccgcg tcgtgttaat caaactggcg atgcgtacgc
      541 gcaccctgca atttttaagc aacgcccccg acagccccga aaaacgcgcc gtcgccaaag
      601 aaaccctcga catcttcgcc ccgctcgcca accgtttggg cgtgtggcag ctcaaatggc
      661 agctcgaaga tttgggcttc cgccatcaag agcccgaaaa ataccgcgaa atcgcgctgc
      721 ttttggacga aaaacgcacc gaacgcctcg aatacatcga aaacttcctc aacatcctgc
      781 gcggtgaact caagaaatac aatgtccatt tcgaagtcgc cggccgcccg aaacacatct
      841 actccattta caaaaaaatg gtgaagaaaa aactcagctt cgacggcctc tttgacatcc
      901 gcgccgtgcg gattctggtt gataccgtcc ccgagtgtta caccacgctg ggcattgtcc
      961 acagcctttg gcagcctatc cccggcgagt tcgacgacta catcgccaac ccgaaaggca
     1021 acggctataa aagtttgcac accgtcatcg tcggtcccga agacaaaggt gtggaagtgc
     1081 aaatccgcac cttcgatatg caccaattca acgaattcgg tgtcgccgcc cactggcgtt
     1141 acaaagaagg cggcaaaggc gattccgcct acgagcaaaa aatcgcctgg ttgcgccaac
     1201 ttttggactg gcgcgaaaat atggcggaaa gcggcaagga agacctcgcc gccgccttca
     1261 aaaccgagct gttcaacgac acgatttacg ttttaacccc gcacggcaaa gtcctttccc
     1321 tgcctacggg cgcgacaccc atcgacttcg cctacgccct gcacagcagc atcggcgacc
     1381 gctgccgggg cgcgaaagtc gaagggcaaa tcgtgccgct gtccaccccg cttgaaaacg
     1441 gacagcgcgt cgaaatcatt accgccaaag aagggcatcc ttccgtcaac tggctgtacg
     1501 aaggctgggt caaatccggc aaggcaatca gtaaaatccg cgcctacatc cgccagcaaa
     1561 acgccgacac cgtgcgcgaa gaaggccgcg tccaaatcga caagcagctt gccaaactca
     1621 cgcccaagcc caacctgcaa gagcttgccg aaaacctcgg cttcaaaaaa ctcgacgacc
     1681 tctacactgc cgtcggacaa ggcgaaattt ccaaccgcgc catccaaaaa gcctgcggca
     1741 cgctgaacga acctccgccc gtacccgtca gcgaaaccac catcgtcaaa cagtccaaaa
     1801 tcaaaaaagg cggcaaaaac ggtgtgctca ttgatggcga agacggtttg atgaccacgc
     1861 ttgccaaatg ctgcaaaccc gcgccgcccg acgacattgt cggcttcgtt acccgcgatc
     1921 gcggcatttc ggtacaccgc aaaacctgcc cctctttccg acacctcgcc gaacaagcac
     1981 ctgaaaaagt gctggacgca agctgggcgg cgttgcagga aggacaagtg tttgccgtcg
     2041 atatcgaaat ccgcgcccaa gaccgctccg ggcttttgcg cgacgtatcc gacgcgctcg
     2101 cccgccacaa actcaacgtt accgccgtgc aaacccagtc ccgcgacttg gaagccagca
     2161 tgaggttcac gctcgaagtc aaacaagtta ccgacctccc acgcgtcctc gccagcctcg
     2221 gcgacgtcaa aggcgtattg agcgttaccc ggctttaaat acaaaaatgc cgtctgaaag
     2281 ccgtataacg cttcagacgg cattttgatt gccggggttt gctatttttt gttgcataat
     2341 caat
//
LOCUS       DAXHSJ010000210         2344 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000210 DAXHSJ010000000
VERSION     DAXHSJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.210"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            33..2183
                     /gene="yccS"
                     /locus_tag="ACFM8U_001979"
     CDS             33..2183
                     /gene="yccS"
                     /locus_tag="ACFM8U_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC6335153.1"
                     /translation="MKTPPLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLVAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVETLNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT          557 a          909 c          468 g          410 t
ORIGIN      
        1 tattcacttc ataacaataa accggtaaaa ccatgaaaac cccacccctc aagcctctgc
       61 tcattacctc gcttcccgtt ttcgccagtg tttttaccgc cgcctccatc gtctggcagc
      121 taggcgaacc caagctcgcc atgcccttcg tactcggcat catcgccggc gggctggtcg
      181 atttggacaa ccgcctgacc ggacggctga aaaacatcat cgccaccgtc gccctattca
      241 ccctctcctc acttgttgca caaagcacgc tcggcacagg cctgcccttc atcctcgcca
      301 tgaccctgat gacctttggc ttcaccattt taggcgcggt cgggctgaaa taccgcacct
      361 tcgccttcgg cgcactcgcc gtcgccacct acaccacact tacctacacc cccgaaacct
      421 actggctgac caaccccttc atgattttat gcggcaccgt actgtacagc accgccatca
      481 tcctgttcca aatcatcctg ccccaccgcc ccgtccaaga aagcgtcgcc aacgcctacg
      541 acgcactcgg cggctacctc gaagccaaag ccgacttctt cgaccccgat gaggcagcct
      601 ggataggcaa ccgccacatc gacctcgcca tgagcaacac cggcgtcatc accgccttca
      661 accaatgccg ttccgccctg ttttaccgtt tgcgcggcaa acaccgccac ccgcgcaccg
      721 ccaaaatgct gcgctactac ttcgccgccc aagacatcca cgaacgcatc agctccgccc
      781 acgtcgacta ccaagagatg tccgaaaaat tcaaaaacac cgacatcatc ttccgcatcc
      841 accgcctgct cgaaatgcag ggacaagcct gccgcaatac cgcccaagcc ctgcgcgcaa
      901 gcaaagacta cgtttacagc aaacgcctcg gccgcgccat cgaaggctgc cgccaatcgc
      961 tgcgtctcct ttccgacggc aacgacagcc ccgacatccg ccacctgcgc cgtcttctcg
     1021 acaacctcgg cagcgtcgac caacaattcc gccaactcca gcacaacggc ctgcaggcag
     1081 aaaacgaccg catgggcgac acccgcatcg ccgccctcga aaccggcagc ctcaaaaaca
     1141 cctggcaggc aatccgtccg cagctaaacc tcgaatcagg cgtattccgc catgccgtcc
     1201 gcctgtccct tgtcgttgcc gccgcctgca ccatcgtcga aaccctcaac ctcaacctcg
     1261 gctactggat actgctgacc gcccttttcg tctgccagcc taactacacc gccaccaaaa
     1321 gccgcgtccg ccagcgcatt gccggtaccg tactcggcgt aatcgtcggc tcgctcgtcc
     1381 cctacttcac cccgtctgtc gaaaccaaac tctggattgt catcgccagt accaccctct
     1441 ttttcatgac ccgcacctac aaatacagtt tctccacctt cttcattacc attcaagccc
     1501 tgaccagcct ctccctcgca ggtttggacg tatacgccgc catgcccgta cgcatcatcg
     1561 acaccattat cggcgcatcc cttgcctggg cggcagtcag ctacctatgg ccagactgga
     1621 aatacctcac gctcgaacgc accgccgccc ttgccgtatg cagcaacggt gcctatctcg
     1681 aaaaaatcac cgaacgcctc aaaagcggcg aaaccggcga cgacgtcgaa taccgcgcca
     1741 cccgccgccg cgcccacgaa cacaccgccg ccctcagcag caccctttcc gacatgagca
     1801 gcgaacccgc aaaattcgcc gacagcctgc aacccggctt taccctgctc aaaaccggct
     1861 acgccctgac cggctacatc tccgccctcg gcgcataccg cagcgaaatg cacgaagaat
     1921 gcagccccga ctttaccgca cagttccacc tcgccgccga acacaccgcc cacatcttcc
     1981 aacacctgcc cgaaaccgaa cccgacgact ttcagacagc actggataca ctgcgcggcg
     2041 aactcgacac cctccgcacc cacagcagcg gaacacaaag ccacatcctc ctccaacagc
     2101 tccaactcat cgcccgacag ctcgaaccct actaccgcgc ctaccgccaa attccgcaca
     2161 ggcagcccca aaatgcagcc tgaaaaagtt tcggcatttt gtaagagagg acagattgtc
     2221 agacaggttg caagatagtg gataaggctt tgcgcagggt aaatgcgtag caactgaacc
     2281 gtcattccca cgaacctaca tcccgtcatt cccacgaaaa cagaaaacca aaaacagaaa
     2341 ccta
//
LOCUS       DAXHSJ010000211         2334 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000211 DAXHSJ010000000
VERSION     DAXHSJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.211"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            144..530
                     /locus_tag="ACFM8U_001980"
     CDS             144..530
                     /locus_tag="ACFM8U_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6335154.1"
                     /translation="MKGLDKYILFKNCETLGELFRIYQDYLSLNIDDPNLKIKFFDLL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRKIWPDMEEMLLYFPDNPWHYVEWF
                     WWGATCPIELTQLPKIEIYEEQMKQA"
     gene            558..1883
                     /locus_tag="ACFM8U_001981"
                     /pseudo
     CDS             558..1883
                     /locus_tag="ACFM8U_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            2211..>2334
                     /locus_tag="ACFM8U_001982"
     CDS             2211..>2334
                     /locus_tag="ACFM8U_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6335155.1"
                     /translation="MSTFFQQTAQAMIARHIDRFPLLKLDQVIDWQPIEPYLNRQ"
BASE COUNT          818 a          541 c          529 g          446 t
ORIGIN      
        1 ccttggaaag gaacgaccat cattttaagg aatttttcaa ctagccgaga acaaactgga
       61 gcaaaatgga cgattgagtt tcaaaatcat ccattagtag gtaggaaaaa aatggaatta
      121 aaatttagat aaggaattga ataatgaaag gtttagataa gtatatccta tttaaaaatt
      181 gtgaaacatt aggagaattg tttcgaatat atcaagacta tctatcatta aatattgatg
      241 acccaaattt aaaaattaaa ttttttgacc ttttggaaga acttttatca gatggtgtta
      301 ttgaattatg cgattatcgt gaaaatcctc caaaaatatt aaccggttcg ccaaaaacac
      361 aggtagatga attaagaaaa atttggccgg atatggaaga gatgttgcta tattttccag
      421 ataacccttg gcattatgtt gaatggtttt ggtggggagc aacttgccct attgaactga
      481 cacaattgcc taaaatagaa atttacgaag aacaaatgaa acaagcttaa agccagcgta
      541 accagcctgt atgacaaggt gcaggtgaac aatccaatat ccacattacc ggctcagatg
      601 ttgccggcaa agaaggaacc ggcctgattg cagataagca aatccatctg caatcagccg
      661 agcaaagtaa taccgaacgc agccaaaaca aatcggcagg ctggaacgca ggtgctgccg
      721 tatcattcgg acaaggaggc tggtcattag gcgttaccgc aggcggcaat atcggcaaag
      781 gccacggcaa tggcgacagc atcacccacc gccatagcca tatcggcgac aaaggcagcc
      841 aaacccttat ccaaagtggt ggcgatacta ccatcaaagg cgcgcaagta cgcggcaaag
      901 gcgtacaagt caatgccaaa aacctaagta ttcaaagcgt acaagatagc gaaacctatc
      961 aaagcaaaca acaaaacgcc agtgcacaag tgaccgtcgg ttatggcttc agtgccagtg
     1021 gcgattacag ccaaagcaaa atccgagcca atcatgcttc ggtaaccgag caaagcggta
     1081 tttatgccgg agaagacggc tatcaaatca aggtcggaaa ccatacagac ctcaaaggcg
     1141 gcatcatcac cagtacccaa agcgcagaag acaagggtaa aaaccgattc agcacaggca
     1201 cactcgccgg cagtgatatt caaaattaca gccaatacga aggaaaaagt tttggattgg
     1261 gcgcaagtgc gtccataagc ggcaaaacac tgggacaggg cgcacaaaat aagcctcaaa
     1321 acaaacacct gacaagcgtg gccgataaaa acggcgcaag ttcatcagta ggctacggca
     1381 gcgacagcga cagccaaagc agcatcacaa aaagcggcat caatacccaa aacattcaaa
     1441 tcaccgacga agccgcacaa atccggctga caggaaaaac agcggcacaa accaaagccg
     1501 atattgatac aaacgtaacc acagacaccg ccgaacgaca ttcgggcagc ctgaaaaacg
     1561 tatttgacaa agatagagtg caaagtgaac tggatttaca aagaaccgtc agccaagatt
     1621 ttagtaaaaa tgttcaacaa gccaataccg agattaacca acatttagac aaactcaaag
     1681 cagacaaaga agcagccgaa acagcagcag ccgaggcatt agccaatggc gatatggaaa
     1741 ctgccaaaca caaagcccat gaagctcaag atgcggcagc aaaagcagat aattggcaac
     1801 aaggcaaagt cattctcaac atgttagcct caggtttagc tgcgccgacc caaagcggag
     1861 cgggcatcgc tgcggctacc gcatcgccag ccgtatcgta tgcgattgga cagcacttta
     1921 aagatttagc cggtcaaaac gcgaatggca aactaaccgc cagtcaagaa accgctcacg
     1981 ttcttgccca cgcggtatta ggagcagcgg ttgcagcagc aggaggcaac aatgccccgg
     2041 cgggagcatt gagtgcgggc gggtcggaag cggctgcgcc ttacatcagc aaatggttat
     2101 atggcaaaga aaaaggaagt gacttaacgg cggaagagaa agagactgag acctttgcaa
     2161 aattccccaa aatcccctaa attcccacca tgacatttag gggattttcc atgagcacct
     2221 tcttccagca aaccgcccaa gccatgattg ccagacacat cgaccgtttc ccgctattga
     2281 agttggacca agtgattgat tggcagccga tcgaaccata cctgaaccgt caaa
//
LOCUS       DAXHSJ010000212         2315 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000212 DAXHSJ010000000
VERSION     DAXHSJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.212"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..574)
                     /locus_tag="ACFM8U_001983"
     CDS             complement(<1..574)
                     /locus_tag="ACFM8U_001983"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC6335156.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRF"
     gene            737..2143
                     /gene="dnaB"
                     /locus_tag="ACFM8U_001984"
     CDS             737..2143
                     /gene="dnaB"
                     /locus_tag="ACFM8U_001984"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC6335157.1"
                     /translation="MNDYTAMPSEDREIGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          574 a          592 c          614 g          535 t
ORIGIN      
        1 caaaacgttt ggcgacttcg tcccagttga cgatttccca aaaacctttc aggtagttgg
       61 gacggctgtt gcggtagtcg atgtaatagg cgtgttccca cacgtcgcag gtcagcagcg
      121 gcgtgttttc agtggtcagc ggagttgctg cgttggaagt ggaaaccaaa tccaatccgc
      181 cggcaggggt tttcaccagc catgcccaac cggagccgaa agtaccggca gcgcaggcat
      241 tgaacgcttc ttggaatttc tcgaagctgc cccatttcgc gtcgatggcg gcggccagtt
      301 cgccggaagg tttgccttga cctttgggcg tgaaacccag ccagtagaag gtgtggttcc
      361 aagtttgcgc tgcgttgttg aacacgccgc ccgaagattt tttcacaatc tcttccaaag
      421 gcaggttttc aaattcggta cctttgattt gattgttcag gttggtgatg taggtttgat
      481 ggtgtttgcc gtagtggaat tccaaagttt ctttgctcag atgcggggac aatgcgtcca
      541 gttcataagg cagttgcggc agcttatgtt ccattttgtg ctcctaatat tgttttaaat
      601 gttgtatttt ggcagtgttg ctgcaaatag ctcagcagcg cacatatttt acctgttttg
      661 agggatggaa accaattaaa cctgctttac gctataatag aagattgcaa tttcggcacg
      721 acagatagga tgtaccatga acgattacac agccatgccg tctgaagacc gtgagatcgg
      781 cgcattgtcg cttccgccgc actcaatgga ggcggaacaa tccgttttgg gcgggttgat
      841 gctggaaaat ccggcatggg acaggattgc cgatgtggtt tcgggagagg atttctaccg
      901 gcatgaacac cgcctgattt tccgttccat tgccaaatta atcaatgaga gccgtcccgc
      961 agatgtgatt acggttcagg aagatttgca gcggaacgaa gaattggaag cggcaggcgg
     1021 attcgaatac ctgattacgc tggcgcaaaa caccccgtct gccgccaaca tccgccgcta
     1081 cgccgaaatc gtgcgcgagc gttccattat gcgccaactc gccgaagtgg ggacggaaat
     1141 cgcccgcagc gcctacaatc cgcaaggcag ggacgcgggg cagcttttag acgaggcgga
     1201 aaacaaagta ttccaaatcg ccgaaagcac cgccaaatcc aagcagggct ttttggagat
     1261 gcccgatttg ctgaaagaag tcgtacagcg catcgatatg ctctactcgc gcgacaatcc
     1321 cgatgaagtt accggcgtgc cgacagggtt catcgacctc gacaaaaaaa cctcgggtct
     1381 gcaacccggc gacctgatta tcgttgccgg tcgtccgtct atgggtaaga ccgccttttc
     1441 catcaatatc gccgaacacg ttgccgtaga aggcaagctg ccggttgccg tcttctcaat
     1501 ggagatgggc ggtgcgcaac tggtcatgcg tatgctcggc tcggtcggac gcttggatca
     1561 aagcgtcctg aaaaccggca ggctcgaaga cgaacactgg ggtcgtctga acgaagcagt
     1621 cgtcaaactc tccgacgcgc ccgtgtacat cgacgagacc ccgggtttga ctgcgctcga
     1681 actgcgcgcc cgcgcccgcc gtctcgcccg tcaatttaac aataagctgg gattgatcgt
     1741 catcgactac ctgcaactga tggcgggatc cggccgttcc gacaaccgag cttcagagct
     1801 gggcgaaatt tcacgttcgc tcaaagcgtt ggcgaaagaa ttgcaagtcc ccatcatcgc
     1861 cctgtcgcaa ttgagccgca cggtcgaatc gcgtaccgac aaacgcccga tgatgtccga
     1921 ccttcgtgaa tccggtgcta tcgagcagga tgccgacctg attatgttta tgtaccgcga
     1981 cgaatactac aaccaagact cgcccatgaa aggccttgcc gaatgtatca tcggcaaaca
     2041 ccgcaacggt cccgtcggta aaatcttcct cacatggacg ggacaattca ccaaattcga
     2101 caatgctgcc tatattcccg aggaggcaaa gatagaggat taaatggcta tataaaaatt
     2161 tattaggcga aatcaggcaa aatcgtttaa aatcatgctt agagattgcc ctaaaaaata
     2221 aaacgcggtc ttgaggcatt tttgcattca gcccgcatat aattgaaaat atagtggatt
     2281 aacaaaaatc aggacaaggc aacgaagccg cagac
//
LOCUS       DAXHSJ010000213         2282 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000213 DAXHSJ010000000
VERSION     DAXHSJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.213"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(26..1435)
                     /gene="dacB"
                     /locus_tag="ACFM8U_001985"
     CDS             complement(26..1435)
                     /gene="dacB"
                     /locus_tag="ACFM8U_001985"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC6335158.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAISLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1600..2166
                     /locus_tag="ACFM8U_001986"
     CDS             1600..2166
                     /locus_tag="ACFM8U_001986"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC6335159.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          519 a          560 c          657 g          546 t
ORIGIN      
        1 atttaatcca ctatattttc ctgtttcagg ctcggcgttc tttgcacatc agtttcgcat
       61 ccagccagcc gtcgccgccg gagatgatgt ttttggcaac gaagttgtcc aagtctggca
      121 gcagggaaat ggcgcgaccg ctgttgatga tgacgaccac cgccatcggt ttgtcgccca
      181 gccaataacc tgcaagggcg cggacattgt tgagcgtgcc ggtttttaag cgcaacagcc
      241 cgccgctttg tttgaagcgg ttgcgtaaag tcccgtctgt gccggcgatg ggcagcgtgt
      301 cgatgaaatc ttgtgcaaac gggctgaaat aagccgtttc caacatttgc gccatcattc
      361 tcgccgttac cctttctttt ctggacagac ccgaaccgtt ttccaaaacc aaatccgcaa
      421 catcgatgcc cgacacggca agttctcgcc ggacggcaga cgctgcctgt tcggaaacgg
      481 cgggcagttt gccgtcgccg ccgagtttga ggaagacgga acgcgcaatc agattgtccg
      541 aacgcttgtt catgtccgtc aaaatttcct tcatcggctt tgagtgtgca acggcaagcg
      601 tctgcgcgcc ttccggcgtg tcggctatgc cgataccgtc tgaaatccgt ccgccgccga
      661 gcagccagtg gttggtaaaa ctttgccgga tcagttcgtc aagcgcgaac atccggacac
      721 cgacaggctt gcccaaacag ctctcgggaa tattgccgcg caatttcagc gtattgtccg
      781 aaaaagatgc acgcatcagt tttttgatcg aagggcaggc agcttgggag gcggtaattt
      841 tcaagttgtt ttgggcgaaa atatgcggca aaggcggatc ggtgaggatg tcggtactgc
      901 cggcggcatt gcgttcggcg cgcaccataa ccataccggc agacagcata gttggattgg
      961 ggggcgtcat aaacggcgaa ccgctgtcgg cttcgaaatc gtcggggctg ccgacttcgc
     1021 cccacaggct gtggtcgagc atcaggtgtc ccgtgatatt gcgtatgcct tgttcgcgca
     1081 actggcgttg gacggcaagc aggttttcct gattgaaaac ggggtcgccg ctgcccgccc
     1141 aatacaggtt tccgtcaagc gtgccgtcgt ttaccgtacc gttgctttta aactcggtcg
     1201 cccagcggta attgctgccg aaggttttga aggcggcaaa cgcggtaacg agtttcattg
     1261 tggaggcggg gttgacgggg acatccgagc ggtggtcaat gatgactttt ccgctgtcaa
     1321 gctcttggac atatacggcg atttcgtttt gcggaatgcg acccgtatcg agcgcgtgtg
     1381 cggcgaggga ggcgagaagc agcagcaggg aggccgctgt tttggggaaa ttcataggta
     1441 aatctgttac ataaaaaaag caattataag gcaaagccgg ataagtggga acgctgtggc
     1501 ggcgggccgg cttgtttgca ggggaaatca catatataat aatcgctatc attatgaaat
     1561 gattgagacg cacaaactta atattcagga ggaatgatta tggctaagaa aatcagtatt
     1621 ttggtgggca gcctgcgccg agcttctttt gcgcgcaaag tggcattgaa tgcggcggag
     1681 atgttccctg aaggctggca ggcggaaatc gtcgaaatcg gacatctgcc gctttacaat
     1741 ttcgattatg acgaccctgc ggtggaagat gtgccgctgc ccgaaagcta cacggttttc
     1801 cgcgaaacga ttaaggcttc ggacggcatt ttgttcgtta cgtccgaaaa taaccgcacc
     1861 attcccgcct gtttgaaaaa tgcggtggac atcggctcga aaccgaatgc cgacgtggcg
     1921 tggaaaaaca aaccggccgg catcatcagc cattccgtcg gcaagatggg cggttacagt
     1981 tcccaaaaaa acctgcgcct tgcgctgtct tatttcgata tgcccgtaac cggacagccg
     2041 gaagtgtttt tgggcaattc gccgacgctg tttgatgaaa acggcaaatt gattgactcg
     2101 gcaagggatt ttgttcagtc atatattaat cagtttgtcg gtttgattga aagaaatgcc
     2161 aaataaacaa accaagtcga aaggccgcaa aaccggttcg ggttttgcgg cttttttata
     2221 tgggctgggc agcgtcaagg tttgccgttt gccggataat gcgcggcaaa tgaccggcgt
     2281 gt
//
LOCUS       DAXHSJ010000214         2218 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000214 DAXHSJ010000000
VERSION     DAXHSJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.214"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..2064
                     /gene="pilC"
                     /locus_tag="ACFM8U_001987"
     CDS             <1..2064
                     /gene="pilC"
                     /locus_tag="ACFM8U_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6335160.1"
                     /translation="PVQNGKAEMYSASVSTYPDSSSSRIFLQNLKRKNDPNKPGRYSL
                     ADLSASDIQSKEPTFTSRQTVIRLDKGVHQIKLQGNEVTGFNGNSNNATFGIVSEGSF
                     MPDASEWKKVLLPWTVRASNDDGQFNTFNKKENNGKPTYSQKYRSRDNSKRERDLGDI
                     VNSPIVAVGGYLATSANDGMVHIFKKGNGDARNYSLKLSYIPGTMPRQYFDNDTSALK
                     DSTLAQELRTFAEKGYVGDRYGVDGGFVLREVERGGKKHVFMFGAMGFGGRGAYALDL
                     TKVDSNNPTAVSLFDVKNDNNNGNNGVKLGYTVGTPQIGKTHNGKYAAFLASGYATKT
                     IDSTDNKTALYVYDLENNNGTPIATINVPDGKGGLSSPTLVDKDLDGTIDIAYAGDRS
                     GKMYRFDLSSQSPDQWTVRPIFEGTKPITSAPAISQLKDKRVVIFGTGSDLSEEDVLS
                     TSEQYIYGIFDDDTATTGTVNFTGTGGGLLEQHLTEENKTLFLTDYKRSDGSGSKGWV
                     VKLKDGQRVTVKPTVVLRTAFVTIHKYTGTDKCGAETAILGINTADGGKLTKKSARPI
                     VPEANQAVAQYSGHKQTAKGKSIPIGCMWKNNETVCPNGYVYDKPVNVRYLDEKKTDG
                     FSTTADGDAGGSGIDPDGKRAGKNNRCFSQKGVRTLLMNDLDSLDITGPTCGMKRISW
                     REIFY"
BASE COUNT          621 a          575 c          569 g          453 t
ORIGIN      
        1 cctgtgcaaa atggtaaggc ggagatgtat tccgcttcgg tttccaccta ccccgacagt
       61 tcgagcagcc gcatcttcct tcaaaatctg aaaagaaaaa acgaccccaa caaacccggc
      121 cgctattccc tcgcagactt gagtgcgtcg gatattcaaa gtaaagagcc gactttcaca
      181 agtcggcaaa ccgtcatccg attggataaa ggcgtacatc agatcaaact tcaaggcaat
      241 gaggtcaccg gttttaatgg aaacagcaac aacgcaactt tcggcattgt tagtgaaggg
      301 agcttcatgc ctgatgccag cgagtggaaa aaagtattgc tgccttggac ggttcgtgct
      361 tccaatgatg acggtcaatt taacacattc aacaaaaaag aaaacaacgg caagccaaca
      421 tacagtcaaa aataccgcag ccgcgataac agcaagcgcg agcgtgattt gggcgacatc
      481 gtcaacagcc ccatcgtggc ggtcggcggg tatttggcta cttccgccaa cgacgggatg
      541 gtgcatatct tcaaaaaagg caacggggac gcgcgcaact atagtctgaa gctcagctac
      601 atccctggca cgatgccgcg ccaatatttt gataacgaca cttccgctct caaagactcc
      661 accctcgccc aagagctgcg cacctttgcc gaaaaaggct atgtgggcga ccgctacggc
      721 gtggacggcg gctttgtctt gcgcgaagtc gaacggggcg ggaaaaaaca cgtgtttatg
      781 ttcggcgcga tgggctttgg cggtagaggc gcatacgcct tggatttgac caaagtcgac
      841 agcaataacc cgaccgccgt ttccctgttt gatgtcaaaa atgacaataa taacggcaat
      901 aatggcgtga aattaggcta caccgtcggc acgccgcaaa tcggcaaaac ccacaacggc
      961 aaatacgccg ccttcctcgc ctccggttat gcgactaaaa ccattgacag cacagacaat
     1021 aaaaccgcgc tgtatgtgta tgatttggaa aacaacaacg gtacgccgat tgcaacaatc
     1081 aacgtacccg acggcaaggg cgggctttca tcccccacgc tggtggataa agatttggac
     1141 ggcacgatcg atatcgccta tgccggcgat cgcagcggga aaatgtaccg ctttgatttg
     1201 agcagtcaaa gtcctgatca atggactgta cgccctattt ttgaaggcac aaaaccgatt
     1261 acttccgcgc ccgccatttc ccaactgaaa gacaaacgcg tggtcatctt cggcacgggc
     1321 agtgatttga gtgaggagga tgtactcagt acgagcgaac aatatattta cggtatcttc
     1381 gacgacgata cggcgacgac gggtactgta aacttcaccg gtacgggagg cgggctgctc
     1441 gagcaacacc ttactgagga aaataaaacc ttattcctga ccgattataa gcgatccgac
     1501 ggctcgggca gcaaagggtg ggtagtgaaa ttgaaggacg gacagcgcgt taccgtcaaa
     1561 ccgaccgtgg tattgcgtac cgcctttgta accatccata aatatacggg tacggacaaa
     1621 tgcggcgcgg aaaccgccat tttgggcatc aataccgccg acggcggcaa gctgaccaag
     1681 aaaagcgcgc gcccgattgt gccggaagcc aatcaggctg tcgcgcaata ttccggccat
     1741 aagcaaaccg ccaaaggcaa atccatccct ataggttgta tgtggaaaaa caatgaaacc
     1801 gtctgcccga acggatatgt ttacgacaaa ccggttaatg tgcgttatct ggatgaaaag
     1861 aaaacagacg gattttcaac aacggcagac ggcgatgcgg gcggcagcgg tatagacccc
     1921 gacggcaagc gtgccggcaa aaacaaccgc tgcttctccc aaaaaggggt gcgcaccctg
     1981 ctgatgaacg atttggacag cttggacatt accggcccga cgtgcggtat gaaacgaatc
     2041 agctggcgtg aaatcttcta ctgatttgca cgcgaaaatg ccgtccgaaa ggttttcgga
     2101 cggcattttt tgcgtttttc gggaggggcg ggttcgtaaa aggcgggcta tagggtaggc
     2161 ttcatctcgc caatctcact gaatccatca atttccacaa ttcaattaaa taccgtca
//
LOCUS       DAXHSJ010000215         2205 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000215 DAXHSJ010000000
VERSION     DAXHSJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.215"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            373..885
                     /locus_tag="ACFM8U_001988"
     CDS             373..885
                     /locus_tag="ACFM8U_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC6335161.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            965..2044
                     /gene="aroB"
                     /locus_tag="ACFM8U_001989"
     CDS             965..2044
                     /gene="aroB"
                     /locus_tag="ACFM8U_001989"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC6335162.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEVHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          535 a          684 c          534 g          452 t
ORIGIN      
        1 ttcagcacct tagagaatcg ttctctttga gctaaggcga ggcaacgctg tactggtttt
       61 tgttaatcca ctatagtatt gataaacata ttatcttcaa tatattcaat tggataattg
      121 tttacctaag caaagataat tgccttttcc tgacaaataa gtgaaatcaa cggattgtca
      181 aaacacagcc tgaaataaaa aacctccctg atttctttta tttttcctta aaatcagaaa
      241 ggttcgggat ggtcgggtta tttttccaaa cgtaccgctg ccctgccgat ttcgtataaa
      301 attccgccgt aacccgacaa gcccgaaccc tgtcgccccg aaaggcgggg tgtcaaacat
      361 taaggaattg tgatgaaaaa ctttaacggc aaactcatcc tcatcggact gatgggcgcg
      421 ggcaaaacca cgctgggccg gcaaatggcg cagcggctgg attaccgttt ttacgacagc
      481 gatcacgaaa tcgccgtagc cgccggcgtt cccatcccca ccatatttga aatggaaggc
      541 gaacagggat tccgttcgcg cgaaaccgcc atactcaaaa agctggttat cctgccccat
      601 atcgtcctgt ccaccggcgg cggcgcggtg ttaaaagaag aaaaccgcgc ccttatccgc
      661 aaaagcggca cggtcgtcta tctgcacgcc ccgcccgaaa ccctgctcga acgcacgcgc
      721 tgcgacaaca gccgtccttt gctgcaagtt gccgatcctt tggcgaaatt acgtgaactc
      781 tacgccgcac gcgaccccgt ttaccgccaa accgccgact ttaccgtaga atcggcaaac
      841 tgccgggaaa ccgtgcaaac cctgctcaaa cgcttatccc gataaaccgg catatgcgcc
      901 gcgcccagaa aaccaagccg cgccccgccc ggcgggccgg cggttcaaac tttaaggaac
      961 aacaatgaaa acactgaccg tacacacccc gtcccacagc taccccatct ttatcggcaa
     1021 cgggctgctg ccgcaggcag gcagcctgct caaaccgcat ttgggcaaac gcgccgccat
     1081 catcaccaac gaaaccgtcg ccccgctcta cctcggcacg cttcagacgg cattggatgc
     1141 ggcaggcgta tcccatttca gcatcatcct gcccgacggc gaggtgcaca aaaactggca
     1201 gacgctcaac ctcatctttg acgggctgat gcaaaaccgc gccgaacgca aaaccacatt
     1261 aatcgcactg ggcggcggcg tgatcggcga catggtcggc tttgccgctg ccacctacca
     1321 gcgcggcgca ccgttcgttc aaataccgac cacactgttg agtcaagtcg actcatcggt
     1381 gggcggcaaa accgccatca accacccgct cggcaaaaat atgatcggcg cattctacca
     1441 gccgcaggcg gtgcttgccg acttggacac gctgcacacc ctgcccgtcc gcgaattgtc
     1501 cgccggtatg gcggaagtca tcaaatacgg cgcgctcggc gacatcggtt ttttcgaatg
     1561 gctcgaacaa aacatatccg gactgatgac gctcgatcgg gaaaaactcg cccaagccgt
     1621 gtaccgctgc tgccaaatga aggcagacat cgtcgcccaa gacgaaaccg aacagggcat
     1681 acgcgcatgg ctcaacctcg gacacacctt cggacacgcc attgaaaccg agatgggtta
     1741 cggcacttgg ctgcatggag aagccatcgc cgccggctgc gtgttggcgg cgcgtttgtc
     1801 cgaacaactg ggcaaaacct ccgccgcaga taccgcgcgg ctcgccgccc tgctcgaagc
     1861 cgctggactg ccgtccgcac cgcccgtgtt tgcctttgaa aaatggctcg aacatatgag
     1921 ccacgataaa aaagtcagcg gcggcatcat gcgctttatc ggtctgaacc ggctgggcga
     1981 agccaacatc accgaaatta ccgacacgga catcctccgc cgcaccctgc aaccgtatct
     2041 ctgatttcct ctgccgatgt gctgccgcgc gggtttgacg cacgatgatg attttccatc
     2101 atctttctcc gtaaaagcgg gaatccggtc cgttcggttt cggtcgtttc cgataaattc
     2161 ctgctgcttt tcatttctag attcccactt tcgtgggaat gacgg
//
LOCUS       DAXHSJ010000216         2194 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000216 DAXHSJ010000000
VERSION     DAXHSJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.216"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            31..516
                     /locus_tag="ACFM8U_001990"
     CDS             31..516
                     /locus_tag="ACFM8U_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335163.1"
                     /translation="MFVNEKYPYAALFAGLVFLTLPFALAVHDAFALAFGRAGLLVSV
                     SAGGFGRRGGWDGTVWFVFGVFAFLNLVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSAALGGLRGEAVLEYAFAVWLVAMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            613..1236
                     /gene="rsmG"
                     /locus_tag="ACFM8U_001991"
     CDS             613..1236
                     /gene="rsmG"
                     /locus_tag="ACFM8U_001991"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC6335164.1"
                     /translation="MERKERLQSGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1335..2108
                     /locus_tag="ACFM8U_001992"
     CDS             1335..2108
                     /locus_tag="ACFM8U_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC6335165.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
BASE COUNT          423 a          502 c          755 g          514 t
ORIGIN      
        1 tgcggtctgt ttcggaagag ggggacggcg atgtttgtga acgagaaata tccttatgcg
       61 gctctgtttg cggggctggt gtttttgacg ttgccgtttg cgttggcggt gcatgatgcc
      121 tttgcgcttg cgttcggacg ggcagggctg ctggtgtcgg tgtcggccgg cggattcggc
      181 cggcgtggtg gttgggacgg cactgtttgg tttgtgttcg gtgtgtttgc gtttttgaac
      241 ctggtcgtgt cggcaggcct gacgaagctg gcgtacaaaa agatgatgcg gcggcattcg
      301 cgttacgcgc tgtttctgtc gggcgtggcg gcttgcgcgg cggcggcggt ggcttggatt
      361 ttcgagctgt tgttgggcag tgccgccttg ggcgggctgc gcggggaggc ggtgttggaa
      421 tatgcgtttg ccgtgtggct ggtggcgatg ctgacgctgc ccaaacgctt gacgcgcgcg
      481 ccagtgcagc cggtagtgtt tcacaggaaa aaataggttg gaacgtgaaa tgccgtctga
      541 aacgccgata cgcggtttca gacggcatgt ttttccgtta acattacgcc tgaacatgga
      601 caggataggg atatggaacg caaagaacgc ctgcaatcgg gcatcgccgc attgggtttg
      661 gatatttccg cagcggcaca ggacaggctt ttggcctatg tggatttgtt gaaaaagtgg
      721 aacaaaacct acaatctgac cgccctgcgc gacgaggaaa aaatgattgt ccatcatctt
      781 ttggacagcc tgacgctgct gccccatatc gagggtgtgc aaacgatgct ggatgtcggt
      841 tcgggcggcg gtcagcccgg cattccggcg gcggtgtgcc gtccggatgt gcaaataacc
      901 cttttggatg cgaatacgaa gaaaacggct tttttacagc aggcggttat cgagttgggg
      961 ttggacaatg tgcgcgtggt atccggacgc gtggaggcgg tttcggacgt gcgtgccgat
     1021 gtggttacca gccgtgcgtt tgcagaactg gcggattttg tgtcgtggac ggtgcatctg
     1081 ttgaaagacg gcggctactg ggcggcgatg aagggcgtgt atccgcagga agaaatcggc
     1141 cgcctgccgc aggatgtgtg cgttgaaaaa gtccaaaggc tcgacgtgcc gggcttggat
     1201 gcggaacgcc atatcgtcat cctgagcaag cgttgagcgt acttcagacg gcatgaatac
     1261 cttttttgtg cggataaagg taaaattccg cactgttttt cttttttcaa catcagacgg
     1321 gacacgggcg ggacatgagt gcgaacatcc ttgccatcgc caatcagaag ggcggtgtgg
     1381 gcaaaacgac gacgacggta aatttggcgg cttcgctggc atcgcgcggc aaacgcgtgc
     1441 tggtggtcga tttggatccg cagggcaatg cgacgacagg cagcggcatc gacaaggcgg
     1501 gtttgcagtc cggcgtttat caggtcttat tgggcgatgc ggacgtgaaa tcggcggcgg
     1561 tacgcagcaa agagggcgga tacgctgtgt tgggtgcgaa ccgcgcgctg gccggcgcgg
     1621 aaatcgagct ggtgcaggaa atcgcccggg aagtgcgttt gaaaaacgcg ctcaaggcag
     1681 tggcggaaga ttacgacttt atcctgatcg actgtccgcc ttcgctgacg ctgttgacgc
     1741 ttaacggctt ggtggcggcg ggcggcgtga ttgtgccgat gttgtgcgaa tattacgcgc
     1801 tggaagggat ttccgatttg attgcgaccg tgcgcaaaat ccgtcaggcg gtcaatcccg
     1861 atttggacat cacgggcatc gtgcgcacga tgtacgacag ccgcagcagg ctggttgccg
     1921 aagtcagcga acagttgcgc agccatttcg gggatttgct ttttgaaacc gccatcccgc
     1981 gcaatatccg ccttgcagaa gcgccgagcc acggtatgcc ggttatggct tatgatgcgc
     2041 aggcaaaggg tgccaaggcg tatcttgcct tggcggacga gctggcggcg agggtgtcgg
     2101 ggaaataggt caatccaaat cgggctgccc gtgcctttat gctgtttggc cgggtgcgtt
     2161 aatatggcgg attaaaataa aaatacttat atcg
//
LOCUS       DAXHSJ010000217         2189 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000217 DAXHSJ010000000
VERSION     DAXHSJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.217"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            481..1938
                     /locus_tag="ACFM8U_001993"
     CDS             481..1938
                     /locus_tag="ACFM8U_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HFC6335166.1"
                     /translation="MSRHPAPTGEKAFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          508 a          637 c          505 g          539 t
ORIGIN      
        1 attttgcaaa ggtctccgac ggtctttata gcggattaac aaaaatcagg acaaggcgac
       61 gaagctgcag acagtacaaa tagtacggaa ccgattcact cggtgcttga gcaccttaga
      121 gaatcgttct ctttgagcta aggcgaggca acgccgtacc ggtttttgtt aatccactat
      181 aatgcgccct tcggcgtggc ggatatataa ggaagtgatt ttccatctaa gtaaaaaccg
      241 ccctatcgga taagccctta acagaaaagg ctttacccgc gccgtatcgg aacacgtact
      301 ctaaaataca atccattgaa ttgaaaaaaa tataaaaaca tccgcccgcg aaaaacggca
      361 gcgcgtcgtt tgacaaagaa tgaaaatatc ggttaaaaac cgattttcat acaaaaaaca
      421 ccgctgccgt ccgcatccgt ttcagacggt attgagagaa aatcttttag gagaaccttt
      481 atgtcccgac atcccgcccc caccggagaa aaagcattct tcggccaccc cttccagctt
      541 tccaccctct tccatatcga attgtgggaa cgtttttcat tttacggaat gcagggcatc
      601 ctgctgattt acctctacta caccgccgac aaaggcggct tgggcataga caaaaccctc
      661 gccggcggca ttgtcggcgc atacagcggc agcgtgtacc tgtccaccat tttgggggcg
      721 tggtttgccg accgcgtatg gggtgcggaa aaaaccctct tcctctcggg catcgtcgtg
      781 atgctcggac acatcgtcct tgccgcagca ccgggcctgt acggcctttt aatcgggctg
      841 atattcatcg cattgggcag cggcggcgtg aaatctacgg ccagttctat ggtgggcgca
      901 ttatacgaac aggacgaaat gcgcccgctg cgcgatgcgg gattttccat tttctacatc
      961 gccatcaata tcggcggctt cctaggcccg ctgctgaccg gcctgctgca ggaaaacatc
     1021 ggtttccatt atggtttcgg tgcggcggcg gtcggtatgg cattcggctt gtggcgttat
     1081 tccctgggac gtaaaaacct gccccacccc accgtccccc atccgctttc aaaaggacag
     1141 ggcaaaactg cggccgccgt cggcatcgcc ctcatcgccg cacttgcaac cgccatcaaa
     1201 accgggcttg tcaacctcga caatttctcc ggcatcctct tatctaccgt catccttgcc
     1261 gtcatcgcct atttcgcccg cctgctgact aatccccgcg tcagttccga caacaaacgg
     1321 cacatcatcg cctacatccc gcttttcctg accatctgta tgttttgggc ggtctggttt
     1381 cagatttaca ccgtggcaac cgtctatttc gacgaaaccg tcaaccgcac catcggttcg
     1441 tttaccgtgc ccgtcgcctg gaaagattct atgcaaagcc tgtgggtcat cctgttttcc
     1501 ggactgatgg cggcaatgtg gacaaaaatg gggcgcaaac agcccaaaac cccgctgaaa
     1561 ttcgctatgg cggtatttgt taccggcgcg tcgtttttgg gattcgtccc ctttatttcc
     1621 gccggcacgc cgatgcctat tgcggttttc gcactgattg tccttgccat cacgataggc
     1681 gaactgatga tttccccgat tgcactgtcc atctccacca aaatcgcacc gcctttattc
     1741 aaaacccaaa tggtcgccct taatttcctt gccttttcat taggcttcac tttgggcggc
     1801 gtattgtttg aaaaaggcta tcaggccggc gacgaaatcg gcttctaccg gctgctgttc
     1861 tacatcggcg cagccacagg cttcctgctg ctcctgctcg tccccaaatt gaacaaaatg
     1921 ctcgaaggca cagactaagt cctaccccga tgcgccgatg ccgtctgaac ccttcagacg
     1981 gcatttttcc cgcataatga aaccaaaccg tttccacccg acaggacagg ctccccccaa
     2041 ccggaaggca gcctgccgat tgtcatttaa atatctgact gattccgtct gtttgcaagg
     2101 gaaatagccg tttatttccg cttgggcgga aaccggttct attgagtaaa aggcatttta
     2161 tccgactaaa ttagtgctgc atcaacgga
//
LOCUS       DAXHSJ010000218         2146 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000218 DAXHSJ010000000
VERSION     DAXHSJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.218"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(239..667)
                     /locus_tag="ACFM8U_001994"
     CDS             complement(239..667)
                     /locus_tag="ACFM8U_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6335167.1"
                     /translation="MTNPSFLAEGQKSAVTEYYLNHGEWPANNSSAGVATSASDIKGK
                     YVQSVEVKNGVITAEMKSSGVNKEIQGKKLSLWAKRQAGSVKWFCGQPVTRAANNTAD
                     DAVKADTNGKIDTKHLPSTCRDASSAVCIETPPTAFYKNT"
     gene            complement(772..1104)
                     /locus_tag="ACFM8U_001995"
                     /pseudo
     CDS             complement(772..1104)
                     /locus_tag="ACFM8U_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1586..1990)
                     /locus_tag="ACFM8U_001996"
                     /pseudo
     CDS             complement(1586..1990)
                     /locus_tag="ACFM8U_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          462 a          465 c          587 g          632 t
ORIGIN      
        1 atgatgaaat tttaagtttt aggaatttat cagaaaaaac agaaaccgct ccgccgtcat
       61 tcccgcaaaa gcgggaatcc aagtctgtcg gtacggaaac ttatcgggaa aacggtttct
      121 ttagatttta cgttctggat tcccgctttt gcgggaatga cgggatttaa gtttcaaaat
      181 ttattctaaa taactgaaac tcaacgaact agattcccgc ttttgcggga atgacggttt
      241 aggtattttt atagaaagcc gtaggtggtg tttctatgca aacggcagat gaagcgtcgc
      301 ggcaggttga cggcaggtgc ttggtgtcga tcttgccgtt ggtgtcggct ttgacggcgt
      361 cgtcggcggt gttgttggcg gcgcgcgtaa ccggctgtcc gcagaaccat tttaccgaac
      421 cggcttgacg cttggcccac agggagagtt ttttgccttg gatttctttg tttacgccgc
      481 ttgatttcat ttcggcggta atgacgccgt ttttgacttc aacgctttga acatatttgc
      541 ctttgatgtc ggaggcggag gttgccacgc cggcagaact gttgttggcg ggccattcgc
      601 cgtgattcag gtaatactct gtgacggctg atttttgacc ttcggccaaa aaggatggat
      661 tcgtcattcc cgcgcaggcg ggaatctagg tctgtcggca cggaaactta tcgggaaaac
      721 ggtttcttga gattttacgt cctagattcc cactttcgtg ggaatgacgg tttaggtatt
      781 tttatagaaa gccgtaggtg gtgtttctat gcaaacgaca gatgaagcgt cgcggcaggt
      841 tgacggcagg tgcttggtgt cgattttgtc ggtgccggtg gcggcggtaa cgtcgtcttt
      901 ggctttggcg gcgcgcgtaa ccggctgtcc gcagaaccat tttaccgaac cgttttgacg
      961 cttggcccac agggagagtt ttttgccttt gatttcgttg tttacgccgc ttgaaagcat
     1021 tgtggcggta atgacgcctt tttcaacttt aacccctgca acatatttgc ctttgatgtc
     1081 ggtggaggat gccacgccgg cggcatcatt aaatcccgtc attcccacga aagtgggaat
     1141 ctagaactca ggaccggaga aaccttttta cccgataagt ttccgtgccg acagacctgg
     1201 attcccgcct gcgcgggaat gacgaagttt ttcggccatg tgccgtgatt cgggcaatac
     1261 tcggtaacgg ctgatttttt ggaagtgttt gaagtcggcg cgtggtgttt ctatgcaatc
     1321 ggcagatgct gcgtcgcggc aggttgacgg caggtgcgac gacatataag tttctgtgcg
     1381 gacgagccta gattcccgct tacacgggaa tgacgggatt aaagtttcaa aatttattct
     1441 aaataactga aactcaacga actagattcc cgcttttgcg ggaatgacga attttaggtt
     1501 gctgtttttg gtttcctgtt tttgtgggaa tgatgaaatt ttaagtttta ggaatttatc
     1561 ggaaaaaaca gaaaccgctc tcccgtcatt cccgcgcagg cgggaatcta ggtctgtcgg
     1621 tacggaaact tatcaggtaa aacggtttct ttagtttggt gtcgattttg tcggtgccgg
     1681 tggcggcggc aacgtcgtct ttggcgttgg cgtcgcgctc aaccggctgt ccgcagaacc
     1741 attttaccga accgtcttga cgcttggccc acagggagag ttttttgcct tggatttctt
     1801 tgtttacgcc gcttgaaagc attgtggcgg taatgacgcc ttttgcgact gtaacttcct
     1861 taacatattt gcctttgatt gttgaagaag atgctacgcc ggcggcacta ttgtcggacg
     1921 gccattcgcc gtgattcagg taatactcgg taacggctga tttttgacct tcggccaaaa
     1981 ggatagcttc gtcattcccg cgcaggcggg aatccaggtc tgtcggtacg gaaacttatc
     2041 gggtaaaacg gtttcttgag attttgcgtt ctagattccc gctttcgcgg gaatgacggg
     2101 attaaagttt caaaatttat tctaaataac tgaaactcaa cgaact
//
LOCUS       DAXHSJ010000219         2005 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000219 DAXHSJ010000000
VERSION     DAXHSJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.219"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            430..1539
                     /gene="fic"
                     /locus_tag="ACFM8U_001997"
     CDS             430..1539
                     /gene="fic"
                     /locus_tag="ACFM8U_001997"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6335168.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCITARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSF"
BASE COUNT          611 a          472 c          423 g          499 t
ORIGIN      
        1 tttctgtttt tgattttctg ttttcgtagg aatgacggtt taaaagttac ccgaaacctc
       61 aaaaaaaagg ctgtgtttta atatagtgtt gatagacgta cttggcttcc atgtattcaa
      121 tcgtggaaat ctatatcttc gtcctcgccg aaatagtcta tgcccgatat acaattttga
      181 tacacaaact tggaaatatg ggtatcgtcg ccggagcgat agaatgcgga cagttatata
      241 tatacggttt ttttagggaa gcgggcagat gaagggcgga ttttgaatta accccatgca
      301 aattgacttt tgcaggcggt tttacctccc atatatttac aaaagccaaa tttttaaaca
      361 tatatccttg atatatacac ggcgtaaaca tatactggaa acatctttaa attttccgaa
      421 attttaaata tgagcaactg gaaacccgat attccctata acgatttacc acccctgccg
      481 ccaaaacagg atattgaaag caaaaccatc ctgaaacgtt gtataaccgc ccgtgcatcc
      541 cttgcccgtt taaagcaggc ggcagaactg ataccgaatc aagccatgct gattaacacc
      601 cttcctgtta tggaagcccg tgcaagttcg gaaattgaaa acatcgtaac cacaacggat
      661 aagctgtttc aatccctgca aatggatacg gaacggcaag accctgccac gaaagaagcc
      721 ctgcaatacc gcaccgccct gtttgcaggc tatgaatcac tgacgagccg ccctttatgc
      781 acacaaaccg ccatcatggt ctgcaacgcc atcaagcacc cctacgaaat ggccatccgc
      841 aaaacaggcg gcacagccct aaaaggaggc aacagcggaa atgttgtcta taccccgccc
      901 gaaggagaag aaaccatacg cggcaagctg gcaaattggg agcggtttat tcacgaaagc
      961 ggcgatttag acccgcttat catcatggcg gcggcacatt accaatttga agccatccat
     1021 ccgtttacgg acggcaacgg gcggacgggg cgcatattga acagcctgct attgattgaa
     1081 aaagggcttt tggatttgcc tattttgtat ttgagccgct acatcatcga aaacagggcg
     1141 gactattacc gcctgctttt aggcgtaacc gaacggcagg attgggaaag ctggataatc
     1201 tacatcttag acggcgtagc cgataccgcc gattggacgg tatcgaaaat agatgcgata
     1261 cgccgcctgt tcgagcagac acgtcaacac atacggacac acgcacaagg aatctacacg
     1321 cacgaactgg taaatcttct gtttgagcag ccatatacac gcattgccaa cctagaagcg
     1381 gcagggatag ccaaacggca gacggcctct aagtacctga aagagctttc agacataggt
     1441 gtgctgcaag aaatcgtcat cggcagggac aaactattca ttcatccgcg cctaatggaa
     1501 ctattgcggg gagagggcaa cagctttact tcattctaac cccctcttcc cccccacatg
     1561 actaacacga aacagggatt ttgacacccg aaccgagacc ccttgtattt cccccgcgaa
     1621 aagccggcat ccgcccgcgt atcatgggag caacaaaacc cctgcctaaa attttgactt
     1681 gtgcaaattg ggggtatatt tgggggtata ttgaaaaatg gctaaaataa aatgtttaat
     1741 aaacaaaatg ttgaaactta atttcgatag agcatctgca tatcgtattg aggcgttcat
     1801 ggaatttgag aaagctattt ttaaataaga aaaggtaact attaaatagc tacctttcta
     1861 aaattaaata tcaacacatc gttaaaacac agcctttatt tttaacaaag ttgcaaatgt
     1921 ttttttattt ttttagggaa tacaccaaaa tcccgtcatt tccgcgcagt cgtgaatccg
     1981 aacgcgtccg catggaaacc tatat
//
LOCUS       DAXHSJ010000220         1841 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000220 DAXHSJ010000000
VERSION     DAXHSJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.220"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..218
                     /locus_tag="ACFM8U_001998"
     CDS             <1..218
                     /locus_tag="ACFM8U_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6335169.1"
                     /translation="HMELETVVPPEEGEGEGEGADGEMPEYLELHGGREDDISAPSQP
                     EPPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            253..1764
                     /gene="ubiB"
                     /locus_tag="ACFM8U_001999"
     CDS             253..1764
                     /gene="ubiB"
                     /locus_tag="ACFM8U_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC6335170.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFADGRRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     DMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
BASE COUNT          410 a          496 c          505 g          430 t
ORIGIN      
        1 tacatatgga gttagaaacg gtcgtgccgc cggaggaagg tgagggcgaa ggagagggcg
       61 cggatgggga aatgcccgaa tatcttgaac ttcatggcgg gcgggaagat gatatttccg
      121 caccttcgca acccgaaccg ccgacccgca atatcaaacc gctgcttttc gggctgctga
      181 ttgccttttt gggctatctg ctcggtaaga ttttttgatt gtccgataaa tgctgtattc
      241 gggattttat atatgaaatg gttgaaacgc ctgacggtca ttgtcgggac tttttaccgc
      301 tatcggctgg caggtctgtg tgcttcgctg atgggtagcg gttggatatg cgctctgctg
      361 aaaatgatgc cgcagtcgtc caaattgaaa aacgaaccgc ctgccgtccg tctgcgcctt
      421 gccttggaaa gcctggggcc gattttcatc aagttcgggc aggttttgtc cacacgcccc
      481 gatttgattc cgcacgatta cgccgtcgaa ctggcaaagt tgcaagacaa agtgccgcct
      541 tttgacgcgc ggctttcgcg tgaacaaatc gaaaaatcgc tgggccagcc catcgaaaag
      601 ctgtatgcgg aatttgaaac cgagcccgtc gccagcgcgt ccatcgccca agtacacaaa
      661 gcccgcctgc attcgggcga acgagtggcg gtcaaagttt tgcgccccaa tcttttgccc
      721 cttatcgaac aggatttgtc gctgatgcgt ttcggcgcgg cttgggtcga gcgtctgttt
      781 gccgacggca ggcgtctgaa gccgcgcgaa gtggtggcgg agttcgacaa atatctgcac
      841 gacgagttgg atttgatgcg cgaagccgcc aatgccggac aacttggacg caatttccaa
      901 aacagcgata tgctgattgt gccgaaggtg ttttacgact actgcaccag cgacgtgctg
      961 accatcgaat ggatggacgg cacgccggtt tccgacatcg ccaaactcaa agcagacggc
     1021 atcgatttgc acaaactcgc cgattacggc gtggaaatct ttttcacgca ggtcttccgc
     1081 gacggctttt tccacgcgga tatgcacccc ggcaatattt tggttgccgc cgacaaccgc
     1141 tacatcgccc tcgatttcgg catcgtcggc acgctgaccg attacgacaa acgctacctc
     1201 gccatcaact tcctcgcctt cttcaaccgc gattaccggc gcgtcgccac cgcccacatc
     1261 gaatcgggct gggtgcctgc cgacacgcgt gcggaagaat tggaagccgc cgtccgcgcc
     1321 gtgtgcgaac cggtgttcaa caaaccgatt tcgcagattt ccttcggctt ggtgctgatg
     1381 cgcctgtttg aagtcagccg ccgcttcaat gtcgaaatcc agccgcagct ggtattgctg
     1441 caaaaaacgc tgctcaacat cgaaggcttg ggacggcagc ttgatcccga tttggacttg
     1501 tggaaaaccg ccaaaccgtt tttggtgaaa tggatgaacg ggcaggtcgg ccctaaagcc
     1561 ctttggcgca acctcaaaaa cgaagccccc gactgggcgc aaatcattcc ttcattgccg
     1621 cgcaaaatca gtgcgttgat tgatgaaaac cgccagcagg aaatgcgtga tgcctatatc
     1681 catttggtca aagtgcagca gcggcaaagc ctgtggctgg cggcgatagc ggttgttttg
     1741 ctgctgattt tgcttttgaa ataggttttg tccgaatcat tgcccgactc cgcccgttta
     1801 taaggaaacc ggttatagtg gattaacaaa aaccagtaca g
//
LOCUS       DAXHSJ010000221         1793 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000221 DAXHSJ010000000
VERSION     DAXHSJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.221"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(458..889)
                     /locus_tag="ACFM8U_002000"
     CDS             complement(458..889)
                     /locus_tag="ACFM8U_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335171.1"
                     /translation="MMFFQIACNSGNSYISLLKSMRFYIDNKECDYIFFRKAVNISDD
                     FIQSGFITPEGLITKNSNPKLFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDA
                     YIEFAYSEDFYVLPEIKIDKRTLKSLNFYIDFGVKYIINKI"
     gene            complement(963..1406)
                     /locus_tag="ACFM8U_002001"
     CDS             complement(963..1406)
                     /locus_tag="ACFM8U_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335172.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQTFENQVFHTQANAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1494..1718)
                     /locus_tag="ACFM8U_002002"
     CDS             complement(1494..1718)
                     /locus_tag="ACFM8U_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335173.1"
                     /translation="MMKNKYILIKAAISITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
BASE COUNT          582 a          316 c          268 g          627 t
ORIGIN      
        1 aactttttgg aagcatctct aatttattaa tatcgttcat atcccctaca cacagaaaaa
       61 atcttgggga atttccttta atctttttaa taaatcttca caaagcctaa aatgatttaa
      121 ttttagtttc tttttatttt tttgataaat tctagataac ctcgcttatt aggaaaaatt
      181 tcaggatata ttcctttttc ttctaaagaa gtatagtgga ttaaatttaa atcaggacaa
      241 ggcgaggaag ccgcagacag tacaaatagt acggcaaggc gaggcaacgc cgtactaatc
      301 cactatatat atccattcat atagagaaaa attcttatac ccaatataaa acaaggtctt
      361 tcattatatt ttgaaaaacc tagaagcttt gtatctattg gtttatcaaa attaataatt
      421 attgcagaag acatgtaggc taataatctt gatttatcta tattttatta attatatatt
      481 taaccccgaa gtcaatatag aaatttagtg attttaaagt tctcttatca attttaatct
      541 cagggagtac ataaaaatcc tcagaatagg caaactctat atatgcatca tcattcgata
      601 ataattcaat aatatttttc atttcatccg aaagaaatgt aaccatctca tattgacatt
      661 tatttttaga taccattttt gaatattgat taaataattt tggattacta tttttagtaa
      721 ttaatccttc cggagtaata aaaccacttt gtataaagtc atcacttata ttaacagcct
      781 tccgaaaaaa aatataatca cactctttgt tatcaatata aaatctcata ctctttaata
      841 atgaaatata agaatttcca gaattacaag caatttgaaa aaacatcatt actcctaaaa
      901 ctcataaata gtaaggaata tatatatatg catattcctt actatttttt cacaaattat
      961 gcttatctat gcttatttcc ataaaaagca tgccctcgaa ttttttgact tgagttctgg
     1021 aaatgagaat aatttgaatg gatttccttc tgtccatgtc taataacatt ttgtcttccc
     1081 caagcttggt ttgctaccgc tcttgcttgt tgagatgttc gtgcatagac attagctcct
     1141 cttgataaag cttgtgctgc aacacgctgc gtaacccaac gctgcgcaag gaatctgccc
     1201 gcatgcaaaa ttcctattgc caccaacggt gccgcagccc cttcagtttc tttcatctcc
     1261 ttttgagaaa gctccgccaa ctgcatcggc gcatttgcct gcgtgtggaa cacttggttt
     1321 tcaaatgtct gattgtccaa tccgtttgcc attgcggggg cgattataga cagcatcatc
     1381 gtggcttcag tgatttgttt tttcataaca aattcttagg gaggagataa tgttccatca
     1441 aaatcctcct gaaaggctgc ctgaaatttc caagcagcct ttttgattgc ttattaatcg
     1501 gatagtgcga caaatataaa gtgggcggca atcattgccg taacggcagc cataactgtt
     1561 ttattttctt caaaccaaag caagttgaga aacataaaca tcaagaaata tgatggttct
     1621 tcagttttgc cgctgctttc ttgagttaga agataaaata tagatatggc ggttatgctg
     1681 attgctgctt ttattaaaat atatttgttt ttcatcatct tgtatggttt ttcatgcaag
     1741 tttcaacaat aaaattcgga ataagataga gagatgttga tatatcagat aaa
//
LOCUS       DAXHSJ010000222         1776 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000222 DAXHSJ010000000
VERSION     DAXHSJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.222"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            50..1219
                     /gene="metZ"
                     /locus_tag="ACFM8U_002003"
     CDS             50..1219
                     /gene="metZ"
                     /locus_tag="ACFM8U_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC6335174.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1314..1520
                     /locus_tag="ACFM8U_002004"
     CDS             1314..1520
                     /locus_tag="ACFM8U_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC6335175.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT          428 a          465 c          463 g          420 t
ORIGIN      
        1 caacgggcgc acgctccgtt gccgaacgga taaaaccgag gaatcaccga tgagcaaaaa
       61 actccatccg caaacgctcg ccatacgcgg cggcaaagaa caaaccgaat accgcgaaca
      121 caatcaggca ttgttcctga ccagcagctt catgtgggac aacgcccaac acgctgccga
      181 tttgttttca aaaaaaatca aaggcttcac ttatacccgt accgccaacc cgaccacagc
      241 cgcctttgaa aaacgcatcg ccgctttgga aggtgcggaa cgcgcggtcg ccacttcgac
      301 cggcatgtcc gcgattcagg cggcgttttt cacctttttg caggcgggcg accatgtgat
      361 ttccagccgc agcctgttcg gcacgaccgt cggctttatc aataacatcg ttaccaaatt
      421 cggcatcgaa gtgagtcacg tgtcgccgac tgatataaac gaatggaaag ccgccgtcaa
      481 agccaacacc aaactgctgt ttttggaaac gccgtccaat cccttgggag aagtggccga
      541 cttggaagcc ttggcggaat tggcgcacgg catcggcgcg ctcttggtgg tggacaacag
      601 cctgctgtcg cctgtcggct ctcagccttt gaaacacggc gcggatattt ccgtttcctc
      661 cgcgaccaaa gccatagacg gacacgggcg cgtgatgggc ggcgtgttgg cgggttcgga
      721 agaactgatg gcgcaggtcg ccgtttactg caactcttgc gggctggcaa tgtcgccgtt
      781 taacgcatgg cagttgttga gcggcgtgga aaccctgtct ctgcgtatgg aaaaacagtt
      841 cgacaacgcc ttgaaaatcg cgcaatggct gcaagcgcag ccgcaggttc aagccgtgta
      901 ttacaccgga ttgcccgacc atccgcaggc ggcgcttatc cgcaaacagc aaaacggcgg
      961 tggcatcgtc atcggctttg aagtagccga ccaagaagcc gcgtggaaag ttgtggacgg
     1021 cgtggaactc ttttcccgaa ccgccaacct cggcgacgtg cgctcgacca tcacccatcc
     1081 gtggacaacc acgcacggca ggatgcagcc cgaagaaaaa ctcgccgcca acatccgccc
     1141 gggactggtg cgcctgtcgg tcggtttgga atatgttggc gatttgattg acgatttgaa
     1201 acaggcactc gcacgctgat tgcgtgttca gacggcatcg gcggaaaaac accgcaaatg
     1261 ccgtctgaaa cccttatctt atatggcata cacgtcaaca ataggaaaaa aatatggatg
     1321 aagcggtttt tgccgattgg gcgttgaaaa tctgcctgac cggtttgatt atttttttag
     1381 gttttatcgt ttggaatttg ggcaaggagt ccaaagcggg caaattcggc atcgccattt
     1441 tgtttttggt tttgggcttg ggcgtgttcg gttttgtgtt caaagagtta ttgattaagt
     1501 ttttggtatt gccgaaatag gtttgcggcc tgtcaaaatg ccgtctgaaa tcataaaaag
     1561 taatctgcac cctaaaagtt tgatttaacc gccaactgat taaggtgcgg atttttatga
     1621 caaaataaaa atcatctttc cgcagtcatt cccacggacg cgaaagtctg tctttttgaa
     1681 tcccggataa ttttacatcg tcattcccgc gcaggcggga atctagtccg ttcggtttca
     1741 gtcatttacg ataaattcct gttgcttttc atttct
//
LOCUS       DAXHSJ010000223         1762 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000223 DAXHSJ010000000
VERSION     DAXHSJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.223"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..953)
                     /locus_tag="ACFM8U_002005"
     CDS             complement(<1..953)
                     /locus_tag="ACFM8U_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335176.1"
                     /translation="MEIFEVNVYTKYNPKDPKQNIIGTVKASYVSDGAGGYLKDKNGN
                     PYIAPVGYNPHEAWAYGAENPLNLLNYGTGKEKDLQRSFHGKSYGGFVPAFQPIASFD
                     VGVACAGAIGVSKDICIAGGGAVNLRNSLSNSNINTEGKYYNNPPNVPNIENGYDWGK
                     NLGTFNDSLENSLKETLNNIRNKFKEIQNSFHNLTDRNISFDSESIGSLLKEKFEDWS
                     HRVYDWLPDDWNLRKELYRNRSGKYHVYDPLALDLDGDGIETVATKGFAGALFDHRNQ
                     GIRTATGWVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADG"
     gene            complement(1541..>1762)
                     /locus_tag="ACFM8U_002006"
     CDS             complement(1541..>1762)
                     /locus_tag="ACFM8U_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6335177.1"
                     /translation="QDGISQTNELRTLEELGIQSLDFAYKDVNKNLGKESKPQSAVLA
                     IHTPFVCFLGNLKGIICTRHNPILYFTKN"
BASE COUNT          594 a          309 c          298 g          561 t
ORIGIN      
        1 ccgtctgcca gtttggtgtt gtcgccgaag agttccgcac cgttgtcgat gatgccgttg
       61 ccgttcaaat cgcggacgag taaaccgtca tcggcagaaa cccaaccggt ggcggtgcgg
      121 atgccttgat tgcggtggtc gaacaatgca cctgcaaagc ctttggtggc aacggtttct
      181 atgccgtctc cgtccaaatc tagggcaagg gggtcgtaaa cgtggtattt gcctgaacga
      241 tttctgtata attctttccg tagattccaa tcatcaggta gccagtcata taccctatga
      301 gaccagtctt caaatttctc cttcaggaga ctgccaatgc tttcagaatc aaaagaaata
      361 tttctatctg ttaaattatg aaatgaattc tgaatctcct taaatttatt cctgatatta
      421 tttaatgttt cttttaatga attttctaaa gaatcattaa atgtaccaag gttttttccc
      481 caatcatatc cattttctat atttggaaca ttaggcgggt tgttatagta tttcccctca
      541 gtattgatat tactattaga taacgaattc cttaaattta ccgctccacc tccagcaatg
      601 caaatatcct tagaaacacc aattgcacca gcacaagcta ctccaacatc aaaagatgca
      661 atgggctgaa acgctggcac aaatccccca tacgattttc catggaatga acgctgcaaa
      721 tctttttctt tacctgtacc atagtttagt agattaagtg gattctccgc tccataagcc
      781 catgcttcat gagggttgta tccgacggga gcaatataag gattaccatt cttatctttt
      841 agataaccac cagctccatc tgaaacatac gaagctttaa ctgtaccaat aatattttgc
      901 ttaggatctt ttggattata ttttgtatat acatttacct caaaaatttc cataactgag
      961 cctttacttg ttgataaaag ataaaataaa attagaaact aaaagtgaga aaaagttaat
     1021 aataataggg atgtataaat gtaaaggttc tgtttcatag ctaaggttat ctgaatatat
     1081 ggaaaaaaag taaaagtcca taaaagtaaa atatatagat aatgataatg ataatgataa
     1141 tgataataga attatcactt tcctagagta aggataatat ttttttacga taaaaaaata
     1201 taaaatagga taaataattg ccatatagat agattttgta gcaaaattaa aaataaataa
     1261 taaaaataat gtaataaagt atattttttg gctatgaaat aaaattgtac ataattgaac
     1321 gagcagatca aaaaatgaac tacatataac aataaataat aacgtattta ccatactaaa
     1381 tttaataggt ctcattatca tatttaataa ccacttcata gtaaatgcag agtgggtgga
     1441 aacactcact ttatggtttg ctacgctctg ctcaattagc aacccgataa cccaatatag
     1501 ataatagggc aattaatcca atctaatttg ttaaaatacg ttaatttttt gtaaaataaa
     1561 gtattggatt atgtcgggtg caaatgatgc cttttagatt gcctaaaaaa caaacaaacg
     1621 gtgtgtgtat agcaagtact gcactttgcg gcttactttc tttaccgaga tttttattta
     1681 catctttata ggcgaaatcc aaagattgaa tacccaattc ttcaagggtg cggagttcgt
     1741 tggtttggga aatgccgtcc tg
//
LOCUS       DAXHSJ010000224         1742 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000224 DAXHSJ010000000
VERSION     DAXHSJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.224"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..918
                     /locus_tag="ACFM8U_002007"
     CDS             <1..918
                     /locus_tag="ACFM8U_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6335178.1"
                     /translation="GSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKP
                     DKTALEKIIQGIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGWTA
                     PLIGTAGKLAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGALSKAKA
                     AKQAIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQPVSAGFEHVLE
                     GHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTVDVGKVIGTTSI
                     KEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            925..1434
                     /locus_tag="ACFM8U_002008"
     CDS             925..1434
                     /locus_tag="ACFM8U_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335179.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
     gene            1503..>1742
                     /locus_tag="ACFM8U_002009"
     CDS             1503..>1742
                     /locus_tag="ACFM8U_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335180.1"
                     /translation="MIYGSMPSEKLTIFQTAFVMQVNIQIPCMLYRRGSVKPPLFEAP
                     RLLPSFTDPVVPKLSAPGGYIVDIPKGNLKTEIEKL"
BASE COUNT          553 a          346 c          367 g          476 t
ORIGIN      
        1 ggcagcgtgg cggcactcaa cggcggcgat gtgaatactg cggcgaatgc ggctgaggtg
       61 gcggtagtga ataatgcttt gaattttgac agtaccccta ccaatgcgaa aaagcatcaa
      121 ccgcagaagc ccgacaaaac cgcactggaa aaaattatcc aaggtattat gcctgcacat
      181 gcagcaggtg cgatgactaa tccgcaggat aaggatgctg ccatttggat aagcaatatc
      241 cgtaatggca tcacaggccc gattgtgatt accagctatg gagtttatgc agcaggttgg
      301 acagctccgc tgatcggtac agcgggtaaa ttagctatca gcacctgtat ggctaatcct
      361 tctggttgta ctgtcatggt tacgcaggct gctgaagcgg gcgcgggaat cgccacgggt
      421 gcggtaacgg taggcaacgc ttgggaagct ccagtaggag cgttgtcgaa agcgaaggcg
      481 gctaagcagg ctataccaac ccagacagtt aaagaacttg atggcttact acaagaatca
      541 aaaaatatag gtgctgtaaa tacacgaatt aatatagcga atagtactac tcgatataca
      601 ccaatgagac aaacgggaca accggtatct gctggctttg agcatgttct tgaggggcac
      661 ttccataggc ctattgcgaa taaccgttca gtttttacca tctccccaaa tgaattgaag
      721 gttatacttc aaagtaataa agtagtttct tctcccgtat cgatgactcc tgatggccaa
      781 tatatgcgga ctgtcgatgt aggaaaagtt attggtacta cttctattaa agaaggtgga
      841 caacccacaa ctacaattaa agtatttaca gataagtcag gaaatttgat tactacatac
      901 ccagtaaaag gaaactaact aaatatgagt aactttgaaa aaaaatatat tttagaatta
      961 aatgatgctt taagccattt aaatcataac tctacctcat ttgatttatt gaaagttttg
     1021 atttcatggt tatcaaacga tattgtcatt gataaattta aaattttagg ttatgacttt
     1081 agtaaatata tcgaaatgaa tcccgatgac tatccggttg aaaaatctat attgaataga
     1141 gaggaaatta tttatctcaa aaacaatatt tatcgtaaaa tatcatcagg aaattttaaa
     1201 tttcaatact ttgtacaata tattagagat attttagaat atttatttat tgaacatatt
     1261 gaaagagtct gtccttactg cgaatggggt gaaatgcaaa aattagaaga acaaaatacg
     1321 catgaaacgg tgtatctctg tactcaatgt ggatgtgctt tttataacga taattcacaa
     1381 tttttattaa aaaccccttt aaccattcca atgaaacgtg atgaatttaa ataaacaagc
     1441 cgtagcctgc atgaacccta aaatccacgt gtagcgtgtg tgcgccagca cgcatgcgtt
     1501 ccatgattta cggctcaatg ccgtctgaaa agctcacaat ttttcagacg gcatttgtta
     1561 tgcaagtaaa tattcagatt ccctgtatgc tgtacagacg cgggagtgtt aagcccccct
     1621 tgtttgaagc tccgcggctc ctgccgagct tcaccgaccc cgttgtgccc aagctctctg
     1681 ctcccggcgg ctacattgtc gacatcccca aaggcaatct gaaaaccgaa atcgaaaagc
     1741 tg
//
LOCUS       DAXHSJ010000225         1729 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000225 DAXHSJ010000000
VERSION     DAXHSJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.225"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(45..362)
                     /locus_tag="ACFM8U_002010"
     CDS             complement(45..362)
                     /locus_tag="ACFM8U_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6335181.1"
                     /translation="MVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVLIV
                     PMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVLQE
                     MFA"
     gene            complement(356..595)
                     /locus_tag="ACFM8U_002011"
     CDS             complement(356..595)
                     /locus_tag="ACFM8U_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HFC6335182.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(718..1515)
                     /gene="truA"
                     /locus_tag="ACFM8U_002012"
     CDS             complement(718..1515)
                     /gene="truA"
                     /locus_tag="ACFM8U_002012"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC6335183.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTAAVRPVQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          393 a          416 c          494 g          426 t
ORIGIN      
        1 aagttaatcc actataatga acacaatcca ttcagactat tcaatcaggc aaacatctcc
       61 tgcaatactg caaacagttt ttcagccgta ctgttgtcta aatttccaag atgtttgacc
      121 aatccggctt tatccacagc cctaatctgt tcgggcaaaa gcaaaccgtc tttatcctga
      181 aagcggacat tgacgcggaa cggggcagga cggcttccgc tcgtcatggg aacgatcagc
      241 acagtcttga gatagttgtg tatttcagga ggagagacta cgacacaagg acgtgtcttt
      301 ttgatttcgc ttcctacggt cgggtctaag gagaccagat agattccgcc gcgtactacc
      361 atatccattc tttatccgct tcgttttcaa tttcggaaaa aaaatgctcc tgctcggttt
      421 cgacaagcat tgcggcagct tctgcccatc ccctgcgaac agtaggacag cttaaaataa
      481 tattgccctt ttcaactgta acagccaagc tgtccactgc ccctatttga cccaataatg
      541 atttgggcag aatcacacct tgcgagtttc ccattttgcg tatgttgaga atcatatacg
      601 taccatattt acctgtttat gtaataacaa tgttagtacc ttgatgaggt agtgtcaaca
      661 tggaaaaaga ttgcctgaca gtttgtccga tttcaaaatc tccgcgacaa gaatgtttta
      721 aagccattcg gggatttggg ggcggatgat gccgtatgcc tcgggatagt cgacgccggt
      781 caggtaaagc ccgtcgggca tgaaggtcgg cggggctttg aggcggctgc gttcttgaat
      841 cagtgcggcg aagccttcga cgctgagtct gccgctgccg acatagacga gcgcacccat
      901 gatgttgcgt accatgtggt gcaaaaaggc gttgccgtgc aaatcgaggc ggacgagtcc
      961 tgagctttgg gtaaggtcgg cgcggtagat ggttttgacg ggggattttg cctggcattc
     1021 ggcggcgcgg aagctggaga agtcttgttc gccgatcaat aaggcggcag cctgccgcat
     1081 ctgcccgatg tcgagtttga ggtgtgtcca gcctgccctg tttttgagca ggggggaacg
     1141 gacgggggcg gattcgagca ggtagcggta atgccgtccg tatgcgtcga agcgggcgtg
     1201 aaattcgggg gcgacctgtc gggcgtgcaa aacggcaatg ccttcgggca ggtgggcatt
     1261 tacgccgcgc acccatgcct gaaccggacg gacggcggca gtatcaaaat gaacgacttg
     1321 ggcagtcgca tgaacgccgg tatcggtcct gccggcaacg gtggtggaaa ccgcttcccc
     1381 tgctatttgg gcgagcgcgg tttccaatgc cgcctgaacg gtcggtacgc cgtcagcctg
     1441 tttctgccag ccgtaaaagc ggctgccgtc ataggatagg gttattgccc agcgttgttt
     1501 ttgtgcggta tccatcggat ttgggattcg gataaatgtt cagactgcat tgtatcgcag
     1561 attttgcagg gaaacggcaa acgcccaggg cgagcggcgt tgtttgggga gttgttgggg
     1621 ggggatgcag ttgctacgaa tcgctatcct gtgaatttac cctgtcagga gtgcccgaat
     1681 cgtcattccc gcgcaggcgg gaatctagga cgtaaaatct aaagaaacc
//
LOCUS       DAXHSJ010000226         1720 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000226 DAXHSJ010000000
VERSION     DAXHSJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.226"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            342..1646
                     /locus_tag="ACFM8U_002013"
     CDS             342..1646
                     /locus_tag="ACFM8U_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC6335184.1"
                     /translation="MKKHIWAASLLPTSLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          354 a          431 c          507 g          428 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggatcggat gcgatatttc catattcaaa caagggattg gcggcacatc ggcaaaatcc
      241 ccgcgccgcg ccggtccggc agggcttgcg tccctcccgg acaagccccg accccgcctt
      301 tccgaaagac gggctcaacc attaaggaaa ctttaatcaa aatgaaaaaa cacatatggg
      361 cggcatcttt gctgccgaca tccctatcgg cagaaccttt aaactggtgg aagccttatt
      421 ccgccgtcaa ttcgggcgat accgcctggg tgatgactgc ggctgccttg gtactgttga
      481 tgacgcttcc cgggctggct ttattctacg gcggtatggt gcggaaaaaa aacctgctct
      541 cgacgatgat gcacagcttt tccatcgcga cattggtggg catcctttgg gtcgccgtcg
      601 gctattcttt agcgttcacg ccgggaaatg cctttatcgg cggtttgggg cgcgtatttt
      661 taagcgggat gcagatagac gctgccgcac agatgctgac cgtgtcgccc aatgcgccga
      721 ctgttcccga accggtattt atgttttttc agatgacgtt tgccattatt tcgaccgcca
      781 ttattaccgg cgcgtttgcc gaacggatga aatattcggc aatgatgctg ttttcgggca
      841 tatggttttt attggtttat gtgccgggcg cgcattgggt gtggggcggc ggctttatga
      901 gcaagggcgg cgtattggat tatgccggcg gtacggtggt gcacatcaat gccggtatcg
      961 cgggactcgt cgccgccttg gttttgggca ggcgcatagg ctacgggcgc gaggcgatgc
     1021 ctccgcacaa tatggcgatg acactgatcg gcgcggcaat gttgtggttc ggctggttcg
     1081 gctttaacgc cggatccgca cttgcggcag acgcggcggc gggtatggcg atggcggtaa
     1141 cgcaggtgtc ggccgtattc ggcgcggcag gctggcttgc ctgcgaaaaa atagcgggac
     1201 acaaaccttc cgctttgggg ctggcttccg gcgcggtttc cggtctggtc ggcatcaccc
     1261 ctgccgccgg ctttaccggc ccgtcgggcg cggccgccat cggtatattg actgccgccg
     1321 cgtgctttgt gtccgtcacc gtcgtcaaac acaaattgcg ttacgatgat tctttggacg
     1381 ctttcggcat acacggattc ggcgggctgg tgggcggaat attgaccggc atctttttcg
     1441 acaaccgcat tttcggcggg gatgcggcag tttggcagca gttgtggata caggtaaaag
     1501 acgggttcgt tatggcggca tacagcgggc tgatgagttg ggcgattttg aaggtcgtgg
     1561 ggaaaatctg cggcggcctg cgcgtcggca aggatgtcga acgcgaaggt ttggatctga
     1621 atatccacgg cgaacgcgtg gaataagggc ggctatgccg tctgaagcct gaaaatcggg
     1681 tttcagacgg catttttcac gtttgccgcc gatggataaa
//
LOCUS       DAXHSJ010000227         1701 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000227 DAXHSJ010000000
VERSION     DAXHSJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.227"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            61..1434
                     /locus_tag="ACFM8U_002014"
     CDS             61..1434
                     /locus_tag="ACFM8U_002014"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6335185.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1614..>1701
                     /locus_tag="ACFM8U_002015"
     CDS             1614..>1701
                     /locus_tag="ACFM8U_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HFC6335186.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYS"
BASE COUNT          437 a          557 c          409 g          298 t
ORIGIN      
        1 tccccatcct atcggtttcc cgcttcagac ggcataaggt ctgaaagaaa gactacaatt
       61 atgagtaatc cattttcctc tttaggtttg ggtacggaac tcgtttccgc actgaccgcg
      121 caaggttacg aaaacccgac gcccatccaa gccgccgcca ttcccaaagc actcgccggt
      181 catgatttac tagccgccgc gcaaaccggc acgggcaaaa ccgccgcctt tatgctgccc
      241 tcgctcgaac gcctcaaacg ttacgccacc gccagcacct cgcccgcgat gcaccccgtg
      301 cgtatgctcg tgttgacccc cacgcgcgaa ctcgccgacc aaatcgacca aaacgtgcag
      361 tcctacatca aaaacctgcc gctgcgccac accgtcttgt tcggcggtat gaatatggac
      421 aaacagaccg ccgatttgcg tgccggctgc gaaatcgtcg tcgccacagt cggacggctg
      481 ctcgaccacg tgaaacaaaa aaacatcagt ttgaacaaag tcgaaatcgt cgttctggac
      541 gaagcagacc gcatgttgga tatggggttt atcgacgaca tccgcaaaat catgcagatg
      601 ctgcccaaac aacgccaaac cctgctcttt tccgctacct tctccgcacc gatacgcaaa
      661 ctggcgcaag acttcatgaa cgcgcccgaa accgtcgaag tcgccgcgca aaacaccacc
      721 aacgccaacg tcgaacaaca catcatcgcc gtcgatacca ttcagaagcg caacctgctc
      781 gaacggctga ttgtcgattt gcatatgaac caggtcatcg tgttctgcaa aaccaaacaa
      841 agcgtcgacc gcgtaacgcg cgaactggtg cgccgcaacc tgtccgcaca ggcgatacac
      901 ggcgaccgtt cccaacaaag ccggctcgaa acactcaacg ccttcaaaga cggcagcctg
      961 cgcgtcctcg tcgccaccga catcgccgcg cgcgggctgg acattgccga actgcccttc
     1021 gtcatcaatt acgaaatgcc cgcccagccc gaagactacg tccaccgcat cgggcgcacg
     1081 gggcgcgcgg gcgcggacgg cgtggcgatt tccctgatgg acgaatccga acagaaaatg
     1141 tttgaatcca ttaaagagct gaccggcaac aagctgctca tcgagcgcat cgagggcttc
     1201 gagccgcaat ggtgggaaca gggcggcgca aaaccggaaa aaaccgaaat gcgcgaaccg
     1261 agacaacgca accgctacga atccgccagg gctcagcgcg aaaaaaatac ccgaccggaa
     1321 accacggcaa acgatgcggg cgcggcttgc ggaaaaattg ccggacgcag ccgccgaagc
     1381 cgtcgggaac accggacgtg cgccctgctc caaccgcgtt acggcgtaaa ataaccctga
     1441 aaatcaaatg ccgtctgaac atttcccgtt tcagacggca tttttcaaac cggactgacg
     1501 catcgggagc aaccgcccgc accggataaa tttcttccgc aaacagtttc agacggcatt
     1561 tgctgcctgt acaatatcat ttccttttcc actcaaacca tcaggatttc ccaatgtcca
     1621 cccaattcga tgtcgccgta atcggcgcag gtccggcagg ctcggtcgca tccgccctgc
     1681 tccgcaaaaa aggttatagt g
//
LOCUS       DAXHSJ010000228         1700 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000228 DAXHSJ010000000
VERSION     DAXHSJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.228"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(195..1367)
                     /gene="porA"
                     /locus_tag="ACFM8U_002016"
     CDS             complement(195..1367)
                     /gene="porA"
                     /locus_tag="ACFM8U_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin PorA"
                     /protein_id="HFC6335187.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEAQ
                     AANGGASGQVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGG
                     ATQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDM
                     PVSVRYDSPDFSGFSGSVQFVPIQNSKSAYTPAHVVVNNKVATHVPAVVGKPGSDVYY
                     AGLNYKNGGFAGSYAFKYARHANVGRDAFELFLLGSTSDEAKGTDPLKNHQVHRLTGG
                     YEEGGLNLALAAQLDLSENGDKAKTKNSTTEIAATASYRFGNAVPRISYAHGFDLIER
                     GKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          435 a          440 c          392 g          433 t
ORIGIN      
        1 tatatgtgca aaccaagcca aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tgctgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgaagcagat
      121 tggcagtcag attgcaatca aagaatgaag gcgagccgtc aaaaacaaag ctatccgctt
      181 caccgccccg atatttagaa tttgtggcgc aaaccgacgg aggcggcatt aatttgagtg
      241 tagttgccga tgccggtatt gcgtttcagc caagcgccag acacgatggc ggaagtgcgt
      301 ttggaaaaat cataatcaac gccggcgatg atttgatcgt agctggtatt ttcgcctttt
      361 ttaccgcgtt cgatcaagtc gaaaccatgg gcatagctga tacgtggaac tgcattaccg
      421 aagcggtagg aagcagtcgc ggcaatttcg gtcgtactgt ttttggtttt ggctttgtcg
      481 ccattttcag acaaatccaa ctgagccgcc aaggcgagat tcaagccgcc ttcctcatag
      541 ccgcccgtca ggcggtgtac ctgatggttt ttcaagggat cggtaccttt ggcttcatca
      601 ctcgtgctgc cgagcaagaa caactcaaaa gcatcacgtc cgacattggc gtgtctcgca
      661 tatttaaagg catagctccc ggcaaatccg ccatttttgt aattcagacc ggcataatac
      721 acatccgatc cgggcttgcc gacaacagcc ggaacgtgag tagcaacctt gttattcaca
      781 acaacatgag ccggcgtata ggcggacttg ctgttttgga tcggaacgaa ttgaacgctg
      841 ccgctgaaac cggaaaagtc cggagagtcg tagcgtacag aaaccggcat atcgtcgtgg
      901 cgtttgaaaa tacccaattg cgaagccaca tcattgttgc tgtcccaagg atcaatggct
      961 tggctggcat cgtcaaactg attcgcaacg cgaccggcgc gcagcgtacc gaattcgcct
     1021 gccaagccga taaaggattc cctgttgccc cactgggtcg cgccgccgcc ggcaacggat
     1081 acgtcttgct caagctgcca aacagctttc agaccttcgc ccaaatcctc gctcccctta
     1141 aagccgataa acgagccgaa atcactgatt ttcgtcctga tgcggctttt ggccttagta
     1201 acttttacct gaccgctcgc tccaccgtta gcggcttgtg cttcagtcaa ttgcagctgg
     1261 tagttcctgc cttccacgcc ggctttgatt tcgccgtata ggctgacatc ggcaacggcc
     1321 gcaagcggca gtgcggacaa tacgagggcg gtaagttttt ttcgcatatc ggcttccttt
     1381 tgtaaatttg ataaaaacct aaaaacatcg ggcaaacacc cgatacgtct tcaattatac
     1441 cccccccccg caaaaaacca tttttcagaa caaatatctg ataaatgccg caacctttat
     1501 tttaaaaatg attatatttt gatataaaac aatagcttat tttttcaaaa acgttgtgtt
     1561 tctacaacac aattcaagcg cagacctcgt gcgagccgat gcgctgctgc ccggatgcag
     1621 tctcggcttt ttaaaacgcc ataaaaaaac acacgcggca ctttatagtg gattaacaaa
     1681 aaccagtacg gcgttgcctc
//
LOCUS       DAXHSJ010000229         1665 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000229 DAXHSJ010000000
VERSION     DAXHSJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.229"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     repeat_region   30..989
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=30..65
                     /rpt_unit_seq="attgtagcactgcgaaatgagaatgggagctacaac"
     gene            1429..>1665
                     /locus_tag="ACFM8U_002017"
     CDS             1429..>1665
                     /locus_tag="ACFM8U_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6335188.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEH"
BASE COUNT          488 a          344 c          403 g          430 t
ORIGIN      
        1 agttgattac atttacggtg cagacggtta ttgtagcact gcgaaatgag aatgggagct
       61 acaacttgat tgcgattgta gggtttgtag gctggattgt agcactgcga aatgagaatg
      121 ggagctacaa catttatttt tctgatgagt ctgttatttt tattgtagca ctgcgaaatg
      181 agaatgggag ctacaacggc tcggcgtggc tccggatgag ttcgtatatt gtagcactgc
      241 gaaatgagaa tgggagctac aacgagcttg attccgtatg gctcgaggcg attattgtag
      301 cactgcgaaa tgagaatggg agctacaacc tgcgccatgg cgtaacgcga tttgacgtca
      361 ttgtagcact gcgaaatgag aatgggagct acaactcgtc atgcggtcag agttgccgaa
      421 cacacattgt agcactgcga aatgagaatg ggagctacaa cgtgcatggt attggtttgt
      481 tttggtaaat tattgtagca ctgcgaaatg agaatgggag ctacaacttt tgatttttta
      541 tattgagcgt ttttgatatt gtagcactgc gaaatgagaa tgggagctac aacgtatttt
      601 ttccgacccc cctgaccccc cttattgtag cactgcgaaa tgagaatggg agctacaacc
      661 ttgtatttaa ttttgaagtt tttaaattta ttgtagcact gcgaaatgag aatgggagct
      721 acaacccctg ctgatgtgtt gcgccgaaac attgtattgt agcactgcga aatgagaatg
      781 ggagctacaa cttggcgcaa gcaactatgt tacgatgaaa aattgtagca ctgcgaaatg
      841 agaatgggag ctacaacgcg ttgcggtcag attacaacaa tgttgagatt gtagcactgc
      901 gaaatgagaa tgggagctac aaccggtttg aacagcagta tatgggaaga aacattgtag
      961 cactgcgaaa tgagaatggg cgctacaacc agccgttgcg ataagcgaac gtgcgttaaa
     1021 gtcattgaga cacagtgcaa ttatggcaag aaaggcgaca gatattgtgt cacagatttg
     1081 aaataagaaa aaaccgccca ctcacagagt aggcggaaaa cttgcgtttt tttgattgtg
     1141 gtcgggttag ctactgcatt gacttaacag caaagcaacc gctaaaatga acttgtggtt
     1201 cacttcaacg gacagcagca actttttccc acgcataagg aatttaagca ttttagcttc
     1261 tcctagtgaa gttaatcaca caggcgagag ccgaacaaaa atccccgttg cagcggggat
     1321 ttttgctgtt ccctgtttga tgattaaact aaaatcttgc aaaatgagac ctttgcaaaa
     1381 ttccccaaaa tcccctaaat tcccaccaag acatttaggg gatttctcat gagcaccttc
     1441 tttcagcaaa ccgcacaagc catgattgcc aaacacatcg accgtttccc gctattgaag
     1501 ttggaccagg tgattgattg gcaaccaatc gaacaatacc tgaaccgtca aaaaaaccgt
     1561 taccttagag accaccgcgg ccgtcccgcc tacccgctgt tgtccatgtt caaagccgtc
     1621 ctgctcggac aatggcacag cctctccgat cccgaactcg aacac
//
LOCUS       DAXHSJ010000230         1636 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000230 DAXHSJ010000000
VERSION     DAXHSJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.230"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(591..1514)
                     /gene="lpxC"
                     /locus_tag="ACFM8U_002018"
     CDS             complement(591..1514)
                     /gene="lpxC"
                     /locus_tag="ACFM8U_002018"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC6335189.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          394 a          391 c          415 g          436 t
ORIGIN      
        1 gggattctag attcccactt tcgtgggaat gacgaatttt aggtttctgt ttttggtttt
       61 ctgtttttga gggaatgatg aaaatttaag ttttaggaat ttatcgggag caacagaaac
      121 cgctccgccg tcattcccgc gcaggcggga atccagcctt gtcggtacgg aaacttatcg
      181 ggaaaacggt ttctttagat tttacgttct ggattcccac tttcgtggga atgacggcat
      241 cagtctgccg tttacagcac ggtttcttta gattttacgt tctagattcc cgcctgcgcg
      301 ggaatgacga atccatccat acgaaaacct gcaccacgtc attcccacga acctacatcc
      361 cgtcattccc acaaaaacag aaacctcaaa tcccgtcatt cccgcgcagg cgggaatcca
      421 gacttgtcgg tgcggacgct tatcggataa aacggtttct tgagattccg cgtcctggat
      481 tcccactttc gcgggaatga cgaattttag gtttctgatt ttggttttct gtttttgtag
      541 gaatgatgaa attttaagtt ttaggaattt accggaaaaa acagaaagcg ttatccacaa
      601 gttctgatgt tcagctcgtg aaatgcgtcg ggcaaatcat cgctgtcggc aaattccacc
      661 cggtcgtaag ccgtttcgtc tgccaaaact gcgcgcaaga gtgcgttgtt gatggcgtgt
      721 cccgatttgt agccttcaaa cgcgccgaca atcggatgtc cgacgatata caaatcaccg
      781 atggcatcaa ggattttgtg gcgcacaaac tcatcgggat agcgcaagcc ttcaggattc
      841 aggacatccg tgtcgtcaat cacgatggcg ttgttcaaat tgccgcccaa acccagattg
      901 tgggcgcgca tcatttccac ttcgtgcata aagccgaaag tgcgcgcgcg cgcgatttcg
      961 tcgatgtagg atttgccggc gaaatcgatt tcaaaagtgg gcgagctgcg gttgaaaacc
     1021 ggatggtcga attcgatggt cagcgttacc ttaaagccgt catacggcgt aaagcgcacc
     1081 catttgcccg cttctttgat ttcgacaggc ttgaggattt tcaaaaaacg cttttgcgcc
     1141 ttttgatcga ccacgcccgc atcttgcaaa aggtaaataa acggcaggct ggagccgtcc
     1201 ataatcggga tttcgggtgc gttcagctca atcagcgcgt tgtcgatacc gtaggcggac
     1261 agcgcggaca taatgtgttc gattgtgccg acgcgcacgc ctttgtcggt aacgatggtg
     1321 gaggaaaggc gggtatcgtt gatcaaataa ggggtcagct tgatttgttc gcccatctcg
     1381 ccgtccaaat cggtacggcg gaaggaaatc ccgctgtttt caggcgcggg gtgcagggtc
     1441 agcgcgacgc gttcgcccga atgcagcccg actccggtaa cgctgatgga tttcgccaaa
     1501 gttctttgca gcataaaccg cttccttatc aatggggtaa gttttggaat aatacgataa
     1561 aaccggaaaa acaggctatg tttttccata gtatttgcca atgtatccgt tttcaatacg
     1621 taagccgcat aaaaat
//
LOCUS       DAXHSJ010000231         1605 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000231 DAXHSJ010000000
VERSION     DAXHSJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.231"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..969
                     /locus_tag="ACFM8U_002019"
     CDS             <1..969
                     /locus_tag="ACFM8U_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6335190.1"
                     /translation="GSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKP
                     DKTALEKIIQSIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGWTA
                     PLIGTAGKLAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGALSKAKA
                     AKQAAPKETINNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRKGAGLLDASNIP
                     ITINGKTIKPVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVLPDGRLANGIIS
                     TGEWAGTKIALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            988..1362
                     /locus_tag="ACFM8U_002020"
     CDS             988..1362
                     /locus_tag="ACFM8U_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6335191.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIEIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
BASE COUNT          503 a          321 c          375 g          406 t
ORIGIN      
        1 ggcagcgtgg cggcactcaa cggcggcgat gtgaatactg cggcgaatgc ggctgaggta
       61 gctgtggtga ataatgcttt gaattttgac agcaccccta ccaatgcgaa aaagcatcaa
      121 ccgcagaagc ccgacaaaac cgcactggaa aaaatcatcc aaagtattat gcctgcacat
      181 gcggcaggtg cgatgactaa tccgcaggat aaggatgctg ccatttggat aagcaatatc
      241 cgtaatggca tcacaggccc gattgtgatt accagctatg gagtttatgc agcaggttgg
      301 acagctccgc tgatcggtac agcgggtaaa ttagctatca gcacctgcat ggctaatcct
      361 tctggttgta ctgtcatggt cactcaggct gccgaagcgg gcgcgggaat cgccacgggt
      421 gcggtaacgg taggcaacgc ttgggaagcg cctgtggggg cgttgtcgaa agcgaaggcg
      481 gctaagcaag ctgctcctaa agaaacaata aacaatttgg caaatttagc caaagcagaa
      541 cagcagattt tattccgtat tgcccaacgc gatacgcaac tggatgcatg gaagacggga
      601 tttaacaata gagtaaggaa aggagcaggc ttgcttgatg caagtaatat tccgataacc
      661 attaacggaa aaaccatcaa acctgtacaa gccataagct taaagggagc acccgtttac
      721 agcggcgtaa gcgaacagga gatttttgcg ctttatcggc agatgactgg ccagaatccg
      781 aattttagag ttttgcctga cggaagatta gcaaatggca ttatcagtac tggagaatgg
      841 gcaggaacaa aaattgcatt aagaaatttt tcaaaaacag agaattcaac tcaagcacga
      901 tggacattag atttgcagaa tcctccatca tttattaaag gtactaaatt ggagcttaaa
      961 ttccaataat ttacaaagga ttttaccgtg gatgagaaac aaaaaattaa gattcttgat
     1021 tttcaaatcg atttatcctc aatttttaac tcttataaaa atcaaatggg tattaatatt
     1081 caagataaaa acttaaaaaa caatttctgt tcttttatgg aagaactgtt aaatgacggt
     1141 tcaatccgtt tacatgatta taccgacggt atcgaaattc ctctaactgg aacttcaaaa
     1201 gaacaagtgc agaaattgaa agacatatgg cctactttgg aagatgccca agcaatatgg
     1261 cctgaagacc cttggtatta cttagaatgg ctttggtggg atattgcgtg tccaatagat
     1321 ttggccgatt tgccgaatat tgatatttat gagcaagcgt aggtatggtt agccgccttt
     1381 agcggcgtaa ccgtacgcat atcagcaaac tttataaaat aacaaggccg tctgaaatct
     1441 gtttttcaac tttttcagac ggccttgcaa cttggcattt cattcgtacg gttacgccgc
     1501 taaaggcggc taaccgtacc tacgagctct gataaaaatg atttatggaa gcaagctgta
     1561 gcctgcatga aacctaaaat ccatgcgtaa ggtgtgtgct tcagc
//
LOCUS       DAXHSJ010000232         1542 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000232 DAXHSJ010000000
VERSION     DAXHSJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.232"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(129..815)
                     /locus_tag="ACFM8U_002021"
     CDS             complement(129..815)
                     /locus_tag="ACFM8U_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC6335192.1"
                     /translation="MYAFTDAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(892..1365)
                     /gene="queF"
                     /locus_tag="ACFM8U_002022"
     CDS             complement(892..1365)
                     /gene="queF"
                     /locus_tag="ACFM8U_002022"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC6335193.1"
                     /translation="MSRNTEELQGISLLGNQKTQYPAEYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     regulatory      complement(1369..1413)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT          387 a          326 c          468 g          361 t
ORIGIN      
        1 gaaacccaac gtaccggatt cccgctttcg cgggaatgac gaagtgggcg ggaatccgga
       61 tttatccgtt ccgacagtgt ttgcaaataa aagaaaaccc aaccgtcccg attcccggca
      121 gggctgtttt acggattttg cagcgagggc gcggggcggt cttgcgcctg tttggtttgc
      181 agggtcgtca gttttttcgt cagcagattc agaatcacgc cgtaggcggg caggaaaaac
      241 agaccgcaga cggtgagttt gaacaggtaa tcgacaaaag cgatgccctg ccagtttgcc
      301 gccataaatt catcgctgct tgcatagaag gcaacggcga aaaataccaa cgtatccaag
      361 gcgttgccga tgacggttga tgcggttggg gcaatccacc acgctttcag acggcgtaat
      421 ttgttgaata caaaaatatc aaggatttgt ccgagcgcat aggcggcaaa gctggctaag
      481 gcgatgcgtc cgacaaaggt gttgaattcg gacagcgcgc ccaagcctgt ccaactgccg
      541 ttgtggaaca aaacggaaaa gacgtaggaa agcaaaaggg cggggaacat cacccaaaag
      601 ataatccgcc gtgccaagtg cgaaccgaaa atgcggacgg tcaggtcggt ggcaaggaag
      661 atgaagggaa aggaaaatgc gccccaagtg gtgtggatgc cgaaaatttg gaaggggaac
      721 tgcaccagat agttgctggc ggcgatgatg aggatatgaa aaagcaccag ccagaagagt
      781 gccttctgtt gctgtgcgtc ggtaaatgcg tacataaaaa aacctttcgg aaaggcgttc
      841 agacggcata tcgtatcgaa ggaatgccgt ctgaaatatg ggaaggatgg tttattgtgc
      901 gtcgtgctca aacaggcgtt tacgcgccaa tgcttcaaac tcggtgcctg ctttgccgta
      961 attggcgaaa ggatgaatgg cgatgccgcc gcgcggtgtg aactcgccga atacttcgat
     1021 gtatttcgga tccatcaggg caatgaggtc tttcatgatg atgttgacgc agtcttcatg
     1081 aaaatcgccg tggttgcgga agctgaagag gtagagtttc agggatttgc tttccaccat
     1141 tttgatgtgc ggaatgtagc ggatgacgat ggtggcgaag tcgggctgcc cggtcatggg
     1201 gcagaggctg gtgaactctg ggcagacgaa tttgacgaaa tagtcgttgt cgggatgttt
     1261 gttgtcgaac gcttccaaaa tttcgggcgc gtattcggcc ggatattggg ttttttgatt
     1321 acccaaaagc gagatgcctt gcagctcttc ggtattgcgg gacatagggt ttccttagtt
     1381 ttttagtgtg ggaggttttc gaaccacggg gcggcgattg taatataagc ggcggtatct
     1441 gtgtagtttt cttcagacgg catggtttgg acggcggcgt tttccgtgtc atatatagtg
     1501 gatgaacaaa aaccagtacg gcgttgcctc gccttagctc aa
//
LOCUS       DAXHSJ010000233         1477 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000233 DAXHSJ010000000
VERSION     DAXHSJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.233"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            27..572
                     /locus_tag="ACFM8U_002023"
     CDS             27..572
                     /locus_tag="ACFM8U_002023"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6335194.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGTGKGNMPLRDAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAAKV"
     gene            569..742
                     /locus_tag="ACFM8U_002024"
     CDS             569..742
                     /locus_tag="ACFM8U_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335195.1"
                     /translation="MKKSLIALALSVLKPQMPEFEIKPASIGYLKQHPSMRLGKSGVA
                     AAKRAARKRKNRR"
     gene            745..909
                     /locus_tag="ACFM8U_002025"
     CDS             745..909
                     /locus_tag="ACFM8U_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335196.1"
                     /translation="MGQVEFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            912..1148
                     /locus_tag="ACFM8U_002026"
     CDS             912..1148
                     /locus_tag="ACFM8U_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335197.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            1120..>1477
                     /locus_tag="ACFM8U_002027"
     CDS             1120..>1477
                     /locus_tag="ACFM8U_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335198.1"
                     /translation="MNLVKLLANNWQPIAIIALIGTGLAVSHHQGYYKSAFAKQQAVI
                     EKMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTERD
                     LCKIPFPPDSRNPNTGF"
BASE COUNT          379 a          344 c          438 g          316 t
ORIGIN      
        1 caatcaatat tttttggaga tgatgaatgg gcaaaaccgt aaccttaacc gctggacaca
       61 gcaacaccga cccgggcgcg gtcaacggaa gcgaccgtga ggcggatttg gcgcaggata
      121 tgcgcaacat tgtggcttca atcctgcgta acgattacgg cttaaccgta cgcaccgacg
      181 gcacgggcaa aggcaatatg cccttgcgcg atgcggtcaa gctgattcgc ggctcggatg
      241 tggcgattga gtttcacacc aacgctgccg tcagcaaagc ggcgacaggc atcgaagcct
      301 tgagtaccgt taaaaacaaa cgctggtgtc aggtgttgag caaagctgtt gccaagaaaa
      361 ccggctggaa actgcgcggc gaagacggct ttaaacccga caatgcgggc cagcattcgc
      421 gcctggctta tgcacaagcc ggcggcattg tgtttgagcc gttttttatc agcaacgaca
      481 ctgatttggc cttgtttaag acgaccaaat ggggcatctg ccgcgcgatt gcggacgcga
      541 ttgcgatgga attgggagcg gcgaaggtat gaaaaagtct ttgattgctt tggctctgtc
      601 tgtcttgaaa ccgcagatgc ctgaatttga gattaagcct gccagtattg gctatttgaa
      661 acaacatccg tctatgcgcc tgggcaagtc gggcgtagcg gctgcgaaac gcgcggcgcg
      721 taaacgcaag aatcgtcgtt aatcatggga caggttgagt tttacgaaaa gatgattggg
      781 ctgtggtcgg ccaaaagccg tgaggcaagt gaacaggcag acttggcggc gtttgaattt
      841 gcggagggcg aactggccaa ttatcaggaa atgctgaaac ggcacctgca aaccaaaagt
      901 gtggaatagc aatgcgtatt ttggatattt ttaaaaaccc ggcgacaggc aatgtgtcgc
      961 actcgaaact gtgggcaaac gttgcctgcg cggcggggac gtttaagttt gtgatgctgc
     1021 ccgatccgtc ggcggagatt tgggcggtgt atttgggcat tgtcggcggc tatgcggtgg
     1081 cgcgctcgtt tgtcagcgtc aaacgtcagg aggtcgagaa tgaatctcgt gaaactgctg
     1141 gcgaataact ggcaaccaat tgccatcatc gcacttatcg gcacgggttt ggcggtgtcg
     1201 caccatcaag gctattataa gtcggcgttt gcgaagcagc aggcggtcat tgagaaaatg
     1261 gaaaaagaca aagcgcaagc cctgctgttg tcggctcaaa actatgcacg cgagctggaa
     1321 caggcgcgtg cggaagctaa aaaatatgaa gtcaaggcac acgctgtcgg catggctttg
     1381 gcgaaaaaac aggcggaagt cagccgtctg aaaacggaaa gagacctttg caaaattcct
     1441 ttccctcccg acagccgaaa cccaaacaca ggttttc
//
LOCUS       DAXHSJ010000234         1464 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000234 DAXHSJ010000000
VERSION     DAXHSJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.234"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            443..>1464
                     /gene="pilC"
                     /locus_tag="ACFM8U_002028"
     CDS             443..>1464
                     /gene="pilC"
                     /locus_tag="ACFM8U_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6335199.1"
                     /translation="MNKTLKRQVFRHTALYAAILMFSHTGGGGAQAETHNYAIVMNEQ
                     NQLKVKQEGSYSTLREKDRERKFIYNRGRQGGGSVFFDNTDTLVSRQSGTAVFGTATY
                     LPPYGKVSGFDERKLKERNNAVDWIHTTRAGLAGYAYTNVICRSSDQCPQLVYETKFS
                     FDGIGLVKNAGRLDRHSDPSRENSPIYKLKDHPWLGVSFNLGSENTVKNGNSFNRLIS
                     SFSEDNNNQTIVSTTRGHLISLGDQQREHTAMAYYLNAKLHLLDKKGIKDIAQGKTVD
                     LGTLRPRVEAKVRRGGNLLNFWATWKIEDKGNITVRLDLPEVKAGRCTNAAHPNSKAK
                     PPSPALT"
BASE COUNT          389 a          396 c          391 g          288 t
ORIGIN      
        1 gcaccgacag gtctggattc ccgctttcgc ggcaatggcg ggccaaggag gcggttgcgg
       61 attgccgaaa accgctttac catgatcagc gcgcaagggt agaataatgc ggtaacccta
      121 tacattgcat ccggccaaag gggcgcgtta cctttgcgaa catccccctt tggcagccgg
      181 gcgaaggggg ctttgcaacc agcaattcgg cgggcgcgga atcgggcggt ttgccgaatc
      241 ccgccgtttg cggcgcgcct gccgccgacg gtatcccgcg aagcaagatt taagggataa
      301 aatatgttcc aacacacagg gcggcacata aggcgccgcc ctgattcgga agggcttgca
      361 cccctcccgg acaaagcctg atcctgccgc cccgaaggac ggatgcccga agggcggggg
      421 gtttgaccga aaaggaaata cgatgaataa aactttaaaa aggcaggttt tccgccatac
      481 cgcgctttat gccgccatct tgatgttttc ccataccggc gggggggggg cgcaggcgga
      541 aacccataac tacgctattg tcatgaacga acaaaatcag ctgaaggtaa agcaggaggg
      601 ttcatattca accttaaggg aaaaagacag ggaacgcaaa tttatctata atagaggcag
      661 gcagggtgga ggctctgtct ttttcgacaa taccgatacc cttgtttccc gacaaagcgg
      721 tactgccgtt tttggcacag ccacctacct gccgccctac ggcaaggttt ccggttttga
      781 tgaaagaaag ctgaaagagc gcaacaatgc cgtcgattgg attcatacca cccgggccgg
      841 gctggcaggc tacgcctaca ccaacgtcat atgcagaagc agcgaccaat gcccccaact
      901 tgtctatgag accaaatttt ccttcgacgg catcggtttg gtaaaaaatg cgggcaggct
      961 ggataggcac tcggacccaa gccgcgaaaa ttcgcccatt tacaaattga aagatcatcc
     1021 atggttgggc gtatctttca atttgggcag cgagaatacc gtcaaaaatg gcaactcatt
     1081 caacagattg atatcttctt ttagtgaaga caataataat caaaccatcg tctctacgac
     1141 acgaggccac cttatttccc ttggcgacca gcagcgcgaa cataccgcca tggcctatta
     1201 tctgaacgcc aaactgcacc tgctggacaa aaaagggatt aaagatatcg cccaaggcaa
     1261 aacagtggat ttgggcacct tgagaccgcg cgtcgaggca aaggtaaggc ggggggggaa
     1321 tctgctaaat ttttgggcta cgtggaagat tgaagataaa gggaacatta cagtccgcct
     1381 cgacctgccg gaagtcaaag ccgggcgctg caccaacgcc gcccacccca attccaaagc
     1441 caaaccccct tcgccggccc tgac
//
LOCUS       DAXHSJ010000235         1460 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000235 DAXHSJ010000000
VERSION     DAXHSJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.235"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..549
                     /gene="mafA"
                     /locus_tag="ACFM8U_002029"
     CDS             <1..549
                     /gene="mafA"
                     /locus_tag="ACFM8U_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6335200.1"
                     /translation="TKNSGRKGERSAGLSVNGTGDYRNETLLANPRDVSFLTNLIQTV
                     FYLRGIEVVPPEYADTDVFVTVDVFGIVRSRTELHLYNAETLKAQTKLEYFAVDRDSR
                     KLLITPKTAAYESQYQEQYALWTGPYKVSKTVKASDRLMVDFSDITPYGDTTAQNRPD
                     FKQNNGKNPDVGNEVIRRRKGG"
     gene            553..>1460
                     /gene="mafB"
                     /locus_tag="ACFM8U_002030"
     CDS             553..>1460
                     /gene="mafB"
                     /locus_tag="ACFM8U_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6335201.1"
                     /translation="MKPLRRLTNLLAACAVAVAAFGQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHG
                     YEEHAPFDNHAADSASKEQGNVNEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKF
                     AAIGGFGSVAGFEKNTREAVDRWIQE"
BASE COUNT          395 a          516 c          337 g          212 t
ORIGIN      
        1 acgaaaaaca gcggacgcaa aggcgaacgc tccgccggac tgtccgtcaa cggcacgggc
       61 gactaccgca acgaaaccct gctcgccaac ccccgcgacg tttccttcct gaccaacctc
      121 atccaaaccg tcttctacct gcgcggcatc gaggtcgtac cgcccgaata cgccgacacc
      181 gacgtattcg taaccgtcga cgtattcggc atcgtccgca gccgtaccga actgcacctc
      241 tacaacgccg aaacccttaa agcccaaacc aagctcgaat atttcgccgt cgaccgcgac
      301 agccggaaac tgctgattac ccctaaaacc gccgcctacg aatcccaata ccaagaacaa
      361 tacgcccttt ggaccggccc ttacaaagtc agcaaaaccg tcaaagcctc agaccgcctg
      421 atggtcgatt tctccgacat taccccctac ggcgacacaa ccgcccaaaa ccgtcccgac
      481 ttcaaacaaa acaacggtaa aaaccccgat gtcggcaacg aagtcatccg ccgccgcaaa
      541 ggaggataaa ccgtgaaacc gctgcgcaga ctgacaaacc tccttgccgc ctgcgccgta
      601 gcggtcgccg ccttcggaca gcccgccctc gcagcggact tggcgcaaga cccgttcatt
      661 accgataaca cccaacggca gcactacgaa cccggcggca aataccacct cttcggcgac
      721 ccgcgcggca gcgtttccga ccgcaccggc aaaatcaacg tcatccaaga ctatacccac
      781 cagatgggca acctgctcat ccaacaggcg gcaatccaag gcaatcttgg ttacaccgtc
      841 cgcttttcag gacacggata cgaagaacac gcccccttcg acaaccatgc cgccgacagc
      901 gcaagcaaag aacaaggcaa cgtcaacgaa ggctttaccg tataccggct caactgggaa
      961 ggacacgaac atcatcccgc cgatgcctac gacggcccga agggcggcaa ttaccccaaa
     1021 cctacgggcg cacgagacga atacacctat cacgtcaacg gcacagcccg cagtatcaaa
     1081 ctcaatccga ccgacacccg cagcatccgg caacgcatat ccgacaacta caacaacctc
     1141 ggcagcaatt tctccgaccg cgccgatgaa gccaacagaa aaatgttcga gcacaatgcc
     1201 aagctcgacc gctggggcaa cagcatggag tttatcaacg gcgtcgccgc cggcgcgctt
     1261 aaccccttta tcagcgcggg cgaagccttg ggcataggcg acatactgta cggaacgcgc
     1321 tatgccatag acaaagccgc aatgcgcaac atcgccccct tgcccgccga gggcaaattc
     1381 gccgccatcg gcggctttgg cagcgtggcg ggctttgaaa agaatacgcg cgaagccgtt
     1441 gaccggtgga tacaggaaaa
//
LOCUS       DAXHSJ010000236         1451 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000236 DAXHSJ010000000
VERSION     DAXHSJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.236"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            27..1364
                     /locus_tag="ACFM8U_002031"
     CDS             27..1364
                     /locus_tag="ACFM8U_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6335202.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRALRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPVGALGVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLDIL"
BASE COUNT          267 a          345 c          394 g          445 t
ORIGIN      
        1 tatcttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtgct tgctgcggcg ggttcggcca tcggtttggg cgcgatttgg aaattccctt
      121 atacggcggg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttactatct
      181 tggtcgccct gcccgttcag cttgccgaat tttatatcgg gcgcacgggc ggtaaaaatg
      241 ccgtcgattc cttcagggct ctgcgtccgg gtacgcaatg gctttgggtc gggcgcatgg
      301 gcgttgccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattga
      361 attatgtcgt ccacagtttt acaggggcaa tccatgcggg agcggacttt gaagccctgt
      421 tcggcacaac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
      481 ttacggtttg ggtggtcaaa ggcgggattt cagacggcat cgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttaggc caggcgtttt ttgccctgag catcggcgtt tccgccatga
      721 ttacctacgc ttcatatttg ggaaaagatc aggatatgtt ccgctcgggg catacgatta
      781 tgtggatgaa cctcttggtt tcgctgttgg caggtttggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg ccggcggtgt
      901 ttatgaaaat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcattt tcgatgttgg aaacggtcat cgccgcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa cacacttggc ttatcggcac ggccattttc attgtcggca
     1081 tcccatccgc gctgtctttc ggcgtatggg gtgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactatgtt atttccgccg tcattatgcc tgtcggcgcg ttgggcgttg
     1201 ccgtatttac cgcctggatt caggacaagc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ctgctgctgt ggctgaatac cttgcgctac cttgccccga
     1321 ttgccattat tattgttttc gtcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaagcc tttcagacgg catttttgct tcaggttcag cctatttcgt
     1441 tcaaagtata g
//
LOCUS       DAXHSJ010000237         1429 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000237 DAXHSJ010000000
VERSION     DAXHSJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.237"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(30..167)
                     /locus_tag="ACFM8U_002032"
                     /pseudo
     CDS             complement(30..167)
                     /locus_tag="ACFM8U_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            474..722
                     /locus_tag="ACFM8U_002033"
     CDS             474..722
                     /locus_tag="ACFM8U_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335203.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIIKNTVSSDE
                     KLLIINDFLSGFLEFDPETGKPVGETLKIEQMIDFFLV"
BASE COUNT          443 a          207 c          296 g          483 t
ORIGIN      
        1 aaatgtgggc tgttttgata aatcagtcat cgtaaacgat gaaaataata ggctgcggta
       61 tttttattaa cgcctactaa ctctgctgcc gttcttgcag ttacacctgc gacaaatagc
      121 tcaatgagtt tattttgttt atactggctt agacgacttt ttctcatagg gataattcta
      181 acttaatttg aatttcccta gttatctagg acagccccaa agtttattgt ctttaaatga
      241 gataacagat aaaaccttat ttaaagaaaa tgtagtttat cgtttcagta agtattaggt
      301 gtgattttta ttaagttatt tgaggttttc ggtaagtgta gatataaggt tgagttataa
      361 ccaatcattc aggctctgtt aggcttggca actcaacctg tgcttgctga aattgggtat
      421 acagttatac gttttaagcg ggcttcgctc cctttatcat ttaagggtaa tttatgaata
      481 ttaaaaatga aatagttgct ttaatttcta aaaaaattga tggggggcaa tatagttctt
      541 tagaaaattt tgctttatcc gtactaaggg aaaaaattat aaaaaatact gtttccagtg
      601 atgaaaaatt attgattatt aatgattttt tatcaggttt tcttgagttt gatcctgaga
      661 ccgggaaacc tgtcggggag acgcttaaaa ttgagcaaat gattgatttc tttttagtct
      721 aaatcataca taccagtgaa caagctaggc ttatatccgg cttttaaata cgattgtcag
      781 gtttatactt gggataaaat caaaaataaa gttgtttaaa ctctagtaga ttaacaaaaa
      841 tcaggacaag gcgacgaagc cgcagacagt acagatagta cggaaccgat tcactcggtg
      901 cttcaacacc ttagagaatc gttctctttg agctaaggcg aagcaacgcc gtactggttt
      961 ttgttaattc actatattgc atggtgcagc attaaatcag tcttctagcg ccgcaatggg
     1021 aaaacataga agataaacga aatggatgat ctaatactat attttttatc aggtatattc
     1081 ggaagtcaag ttgctgaatg tattataaaa aacaaccgtg agataaaagt tccctttatt
     1141 gtgctttacg caatattttt tacgttgatt tatactgtgg ctttgttgtt tctcagtctg
     1201 atctattggg tcaatggtgc ggagattgca tggaagggga taggtatttt cagtatgtcg
     1261 gtcagttttt gtatagtctt ctgtctttat ttgattgaca aggcaggaag atgtaaggat
     1321 aagaaacaat agggatcaga tgcggcagca aacgtagatt gggtctaggc ttaacctgcg
     1381 tttgctgcat ctttggaaga aatagtggat tcgaaaaaat aggaagaat
//
LOCUS       DAXHSJ010000238         1416 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000238 DAXHSJ010000000
VERSION     DAXHSJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.238"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            188..1372
                     /gene="ubiM"
                     /locus_tag="ACFM8U_002034"
     CDS             188..1372
                     /gene="ubiM"
                     /locus_tag="ACFM8U_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC6335204.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDNIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNRHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT          378 a          409 c          311 g          318 t
ORIGIN      
        1 ataccccaca cccgatttga tacacattaa gctgaaagta aaaatccgca tacaccctcc
       61 cctgcatatt tcttcaacag tgggttttga tataatcgcc tatctgttac agatagttca
      121 aacggcattc aaacccttac aaataccgtc tgaaatcatc ggcatccgtc tatgcaaagg
      181 atattttatg agtttacaca gcgacatcct cgtcgtcggc gcaggcccgg cgggtttaag
      241 ttttgcagcc gaacttgccg gcagcggttt gaaagttacc ctgatcgaac gcagcccact
      301 gactgttctg caaaatcctc catatgacgg acgcgaaatc gccctgaccc atttttcccg
      361 cgaaatcatg cagcgtttgg gtatgtggga caacattccc gaaaacgaaa tctacccttt
      421 gcgcgatgcc aaagtgctga acggacgttc cgactaccag ctccactttc cccaaccgac
      481 tgaagcgcgc ggcgaacctg ccgactgttt gggctatctg atttccaacc acaatatccg
      541 ccgcgccgcc tatgaagtcg tatcacaact cgacaatgtc agcattctga ccgatacggt
      601 tgtcaaagaa gtcaaaacat ctgacaacga agcacaagtc attttggaaa acggcaaaat
      661 cctgactgca cgcctgcttt tggcggcaga cagccgtttc tcacaaaccc gccgacaact
      721 cggcatttct tcagatatgc acgactacag ccgaaccatg ttcgtctgcc gcatgaaaca
      781 tactctttcc aatcggcata ccgcttacga atgcttccat tacgggcgca ccatcgcact
      841 gctgccactg gaagaacacc tgaccaatac cgtcattacc gtcgataccg ataaaatcaa
      901 cagcgttcaa aatctttcgc ccgaagaatt ggcggcaagc gtaaaagaac agctcaaggg
      961 caggctcggc gatatggaac tggtcagcag catccaccat tacccacttg tcggtatgat
     1021 tgccaaacgt ttctacggca aacgcagcgc gctgatcggc gatgcggctg tcggtatgca
     1081 ccccgttacc gcacacggtt tcaacttggg tctgtccagc gcagatattt tggcaaaact
     1141 gatactcgaa gccgagcagc gcggtcagga catcggcgcg gcttccttgt tggaaaaata
     1201 cagcagcaaa catatgctgc acgcccatcc gctttatcac gggaccaata tgatgctgaa
     1261 gctgtttacc aacgaaaccg cgccggcaaa actgctgcgc ggtttggtat tgcgtgccgg
     1321 caacaacttc ccgccgctga aaaagctgat taccaaacaa ctgaccggtt gaaaactgcc
     1381 gaaatgccgt ctgaaacgtt tcagacggca tttttg
//
LOCUS       DAXHSJ010000239         1400 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000239 DAXHSJ010000000
VERSION     DAXHSJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.239"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(41..1030)
                     /gene="porB"
                     /locus_tag="ACFM8U_002035"
     CDS             complement(41..1030)
                     /gene="porB"
                     /locus_tag="ACFM8U_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HFC6335205.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAHNGAQA
                     ASVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVDNVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVEDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDD
                     ADYTNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGESKFVSTAGGVGLRHKF"
BASE COUNT          314 a          322 c          372 g          392 t
ORIGIN      
        1 gtctggcgac aggctttttg ttgataccaa tctttgcaga ttagaatttg tggcgcagac
       61 cgacaccgcc ggcagtcgat acgaatttgc tttcgccttt gccttcttgc aaccaaccgg
      121 cagaaaccaa ggcagaagtg cgtttggaga agtcgtattc cgcaccgaca accacttggt
      181 cgtaatcgtt ggtgtagtct gcatcatcaa acgagccttt gaagccgtgg gcgtaagaaa
      241 cgcggggcgt tacgttgccg aagcggtatg ccaaggtagc ggcaacttcg gtttgagagt
      301 tgtgcgaatt gtcttcaacc agtttcgcgt cttgttgctg tacggctacg gaagcgtata
      361 gggcatcatt gtcgtaaccg ctgaccaaac ggtgaatctg gtatttctca atcttcacgt
      421 tatccacatc ctgatgtctt ttataggcac cgccatattg cacgaagaag ccgccgtttt
      481 tgtagttgaa gccggcatgg taagattcgc tgttatgtct gcctgcattg tcgttaagcg
      541 cgtattgtac gctgccgctg aggccggcaa attcgggaga atcgtagcgt acggaaatga
      601 ggcgtgcttc gggttcggca attttgttta cacccaaata gtcgcttttg ctatcccaag
      661 gattgatgtc gccggtgtct ttcaggacgc tgttcaaacg gccgacgcgc aatttaccga
      721 agccgccttt caaaccgatg aaggattggc ggttgcccca accggagtca gtaccggcga
      781 tagatgcctt ttgctcaacc tgccaaatgg ctttcaggcc gttgccgagg tcttcttggc
      841 ctttgaagcc gattttcgaa cccaaatcaa cgatgccggt accggtttca acgctagccg
      901 cctgagctcc attgtgagct acggagcggg aagtttctac gccggctttg atggtgccgt
      961 acagggtaac gtcagccatt gctgcaacag gaagggctgc caaagtcagg gcaatcaggg
     1021 attttttcat tgctgtattc cttttttggt taagaaattt aagcaggccg ggctttccaa
     1081 gccgcttagc tttgcattta ccgccgacgt tttcggcgat tccatgtctg aaactataga
     1141 tgttcctgtc tgaaaaaaca aacttctgcc tgtttttgtg tggaattcga tgtgtatttt
     1201 gaagggcgga tagagatatt gtgggtattt tttgttttat aacatatggt tatttaaatt
     1261 ttttaagatt tgcattttta caacacttac tcgggagggt attggagggc attgcaaacc
     1321 gggggttata aagacggcaa aaaacgcgca cgcggtttct ttcggaacgg gcggatgcaa
     1381 aacccgcccc tgcggcagga
//
LOCUS       DAXHSJ010000240         1357 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000240 DAXHSJ010000000
VERSION     DAXHSJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.240"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..380)
                     /locus_tag="ACFM8U_002036"
     CDS             complement(<1..380)
                     /locus_tag="ACFM8U_002036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335206.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="ACFM8U_002037"
     CDS             complement(438..803)
                     /locus_tag="ACFM8U_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335207.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="ACFM8U_002038"
     CDS             complement(785..1330)
                     /locus_tag="ACFM8U_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335208.1"
                     /translation="MKKQITAAVMMLSMIAPAMANSMDNQIFENQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
BASE COUNT          414 a          309 c          209 g          425 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccctctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat atctgattgt
     1261 ccatgctgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtaattt
     1321 gttttttcat aataactcct ttggattaca aggttgg
//
LOCUS       DAXHSJ010000241         1251 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000241 DAXHSJ010000000
VERSION     DAXHSJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.241"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            234..1187
                     /locus_tag="ACFM8U_002039"
     CDS             234..1187
                     /locus_tag="ACFM8U_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6335209.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNAESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT          196 a          268 c          435 g          352 t
ORIGIN      
        1 accggatgat tcgggctaaa taattcctga ttctttggat atagccaacg gtatgcctgc
       61 tgatgccgtc agaaagggct tgatgggcgt ttgcggttcg ggttaatatt tgtgctgtat
      121 ctgttaattc cggttagtaa aatggggcgg ggacgatgag tggccgtcgg tcggttttgc
      181 acgggcggta ataatgtccg gcgcaaggtg cgccgtttcg ggaggggtgt gtgatgggtt
      241 ttctgattgg tttgagccgc aacctgacgc ggtttacggc tttggtcatt ttgcttgcgt
      301 cggcggttgc gtttatcgga ccggcttcgt ttgcgtgggt taagggcgat gttcaggtct
      361 tggtgctggg catcattatg ctgggtatgg ggatgacttt gggcagggag gactaccgga
      421 ttttggcgca gcgtcctttg gatgtgttta tcggtgcggc ggcgcaatat accgtgatgc
      481 cgttgctcgc catcggtatt gccaagctgt tcgacctttc gccggggctg actttggggc
      541 tggtgttggt ggggacgtgt ccgggcggcg tgtcgtccaa cattatgagt ttccttgcca
      601 aaggcgatgt ggcgttttca gtcggtatga cgacggtgtc tacggtgctg gctccggccg
      661 ttacgccttt gtggatgacc tgcctggtcg gtcagacggt ggatatggac ggttggggga
      721 tgttccggtt tatgctgctg gtcacgcttt tgccggtggt gctgggttcg gcggcaaata
      781 tcctgctgca cggaaaacat tggtttgaaa atgtccgtgc cgttatgccg ggcgtggcgg
      841 tattggcatt tgcctgcatt gtcggcggtg tggcttcggt tcacggacac cgttttgccg
      901 aatcggcgtt ggtaatggtg ttggcgattg cggtacacaa tatcgccggc tatgtgtcag
      961 gctattacgc cgccgcgctg tttggtatgg atacggcaaa aaaacgcacc ttggcaatcg
     1021 aagtcggcgt gcagaatgcc ggtttggcaa cgggattgag cgcgaaattt tttccgggca
     1081 acgccgaatc ggcggttgcg gcggcggtcg cctgcgtgtg gcattcggtt tcggggacgg
     1141 tattgggcaa cttgttcgct ctgtgggaca agcgtcggga cgcttagtcg ggcgtgtccg
     1201 aaccgtccca cgatatatag tggattaact ttaaaccggt acggcgttgc c
//
LOCUS       DAXHSJ010000242         1210 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000242 DAXHSJ010000000
VERSION     DAXHSJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.242"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            23..1126
                     /gene="dnaN"
                     /locus_tag="ACFM8U_002040"
     CDS             23..1126
                     /gene="dnaN"
                     /locus_tag="ACFM8U_002040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC6335210.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPHAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
BASE COUNT          271 a          337 c          336 g          266 t
ORIGIN      
        1 aaaccgattt aaaggagcga acatgttgat tttacaagcc gagcgcgaca gcctgctcaa
       61 gccgctgcaa gccgttaccg gcatcgtcga acgccgccac accctgccca tcctgtccaa
      121 cgtcctgatt gagggaaaag gcggtcagac caaactcttg gcaaccgatt tggaaatcca
      181 aatcgacact gcgggtcccg agggcggcgc gggcgacttc cgcatcacta ccaatgccaa
      241 gaaatttcag gacattttgc gcgcgctgcc tgccggtgcg ctggtgtcgc tggattggga
      301 cgacagccgt ctgacgctga aggcgggcaa atcgcgtttt gccctgcaga ccctgcctgc
      361 cgccgatttt ccgatgatga atgtcggcga ggacatcagc gcgactttct cgctggagca
      421 ggagcgtttc aaaaccatgc tgtcgcaagt gcagtacagc atggcggtgc aggacatccg
      481 ctattatctc aacggtctgc tgatgcaggt tgagggcagc cagttgcgcc ttgtggcgac
      541 cgacggacac cgccttgcct atgcagcctg cgcgattgat gcggatttgc cgcacgccga
      601 agtgattttg ccgcgcaaaa cggtgctgga actgttcaaa ctgttgaaca accccgacga
      661 tccgattcaa atcgagctgc tggacaaaca ggtgcgtttc caatgcaacg gcacgaccat
      721 cgtcagcaag gtcatcgacg gcaaattccc cgatttcaac cgcgtgattc ctttggacaa
      781 cgacaagatt ttcgttttgt cccgtgccga acttttgggt gcgctggaac gtgtgtccat
      841 tcttgccaac gaaaaattcc gtggcgcgcg cctgttcctg caatccggcc tgttgagcgt
      901 cgtgtgcagc aacaacgagc aggaagaagc gcgcgaagaa atcgaaatcg cctatcaggg
      961 tggcgaactc gaagtcggtt tcaatatcgg ctatctgatg gacgtgttgc gcaacatcca
     1021 ttccgacgat atgcagcttg ccttcggcga tgccaaccgc tcgacgctgt ttaccgtgcc
     1081 gaacaatccg aatttcaaat atattgtgat gccgatgcgg atttgacggt tttccggaac
     1141 acgatgcccg tattggagat ataccccgaa ccgtgcagac ggattcgggg ttttgttcgg
     1201 ctgtcggaaa
//
LOCUS       DAXHSJ010000243         1170 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000243 DAXHSJ010000000
VERSION     DAXHSJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.243"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            53..1111
                     /gene="dinB"
                     /locus_tag="ACFM8U_002041"
     CDS             53..1111
                     /gene="dinB"
                     /locus_tag="ACFM8U_002041"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC6335211.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYAGDVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPQKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDERPVEAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCSALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGRLVPKNQQQGLWA"
BASE COUNT          256 a          341 c          327 g          246 t
ORIGIN      
        1 gaatttgccc gatgccggca gtagaatatc ccttttattc tcagcagagc cgatgtcttc
       61 acgcaaaatc atccacatcg acatggacgc attctacgca tcggtagagc tgcgcgaaca
      121 gccgcatttg aaagggcggc cggtggtcgt cgcgtgggag ggtgcgcgtt cggtgatttg
      181 cgccgcatcg tatgaggcac ggcagttcgg gctgcattcc gcgatgtcgg tggcaacggc
      241 gaaaaggctg tgtccgcaag cggtgtatgt gccgccgcat ttcgacttgt accgtcaggt
      301 ttccgcgcag attcacgccg tattcaggcg ttataccgat ttaatcgaac ccctgtcgct
      361 ggacgaagcc tatcttgacg ttacccgtaa tttcaaaaac atcccttacg ccggcgacgt
      421 tgccaaagaa atccgtgccg ccatttttgc ggaaacgggt ttgaccgcct cggcaggcat
      481 cgcgccgaac aagtttttgg cgaaaatcgc gtcggactgg cgcaagccga acgggcagtt
      541 tgtgttgccg ccgcaaaaag tcatggcatt tttggaaacc ctgcctttgg gcaaaatccc
      601 cggagtgggc aaggtaacgc tgaaaaaaat gcagtcgttg ggtatgcgga cggcgggcga
      661 tttgcgccgt ttcgagcgcg gcgagctttt aaaccatttc ggacgctacg gctaccgcct
      721 ctatgatttg gcgcgcggta cggacgaacg ccccgtcgaa gccgaacgcg aacgcctcca
      781 aatctccaca gaaatcaccc tgcccgaaga cctgccgctt gagcaggctg ccggacacct
      841 gccccatctt gccgaagacc tgtggcggca aatcacgcgc aaaaacgtcg aagcccaaag
      901 cgtaacgctc aagctgaaga cctacgattt ccgcatcatc acgcgcacgc tgacttattc
      961 ctccgtattg cccgactgct cagctctgct gcaggctgcg caaatgttga tggcgcgcgt
     1021 cccgccgcag acggaagacg cgttccgcct tatcggtatc ggtgtcggcc gccttgtgcc
     1081 gaaaaatcaa cagcagggct tgtgggcgta aactgattta ccacaccgtc caaaaaacat
     1141 gccgtctgaa cggttttcag acggcatggc
//
LOCUS       DAXHSJ010000244         1132 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000244 DAXHSJ010000000
VERSION     DAXHSJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.244"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            237..908
                     /locus_tag="ACFM8U_002042"
     CDS             237..908
                     /locus_tag="ACFM8U_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6335212.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
     gene            970..>1132
                     /locus_tag="ACFM8U_002043"
     CDS             970..>1132
                     /locus_tag="ACFM8U_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6335213.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWHPIEHYLNRQKTR
                     YLRDHRGRPA"
BASE COUNT          325 a          274 c          243 g          290 t
ORIGIN      
        1 ttttgcaaag gtctcaaacg gatgaaatca accgatattc aaacacagtc atttttagcg
       61 cattttcagc gtatcgttaa tgcggaaaat ttcgtgaaca ggttttttgc acaggtctcc
      121 gcctcgtttc gcgggatggg aaaccgtatt agaaaacgga cgcacgcaac ataaaccccg
      181 aaagtgatga taagatgatg atttaacgta ctgctttaat tatttaagga attatcgtgt
      241 ttcccgacaa atacaagttg agtttggaag aaaatatttt tctggcaaag aaagtattgg
      301 ttgcccaaat tcacaacctc agccgttttg agaattgtca gacgaccttg ttgcagaccg
      361 aacaaattat ccatggcaaa aatgtagcct ccgcgtcact ggaagacatc caaaccatct
      421 tgaacctgaa acgtgcctat caatatgtga tttcgcatat ttcaaatggc gaaccggtca
      481 atatttcact ccttaaaaaa atcaacaaca ttgttgccaa ggacgattct ttggcacccg
      541 gtgatttccg taccggttcg gtcggcgtaa cgctattgga cggttcccgt catgccccga
      601 atccagtgaa ggaaattgaa gtggcccgcg tgttacaaaa tatcggactg caaagcggtt
      661 cgacgacgga ggcagccgtc cgttttatgc tttattgtat gcggcagcag gttttttggg
      721 acggcaacaa acgaacggca accttatttg ccaacggtct gatgatggcg gggggctgcg
      781 gcatcttgga aatctccgaa atgcagatgc cgcaattcaa tgaaaaactg tccgcattct
      841 atcgctccgg cgacgatacc gatatttcca agtttgtgta tcaaaattgt atatcgggca
      901 tagactgaga cctttgcaaa atcccccaaa atcccctaaa ttcccaccaa gacatttagg
      961 agatttctca tgagcacctt cttccaacaa accgcgcaag ccatgattgc taaacacatc
     1021 gaccgcttcc cactattgaa gttggaccag gtgattgatt ggcacccgat cgaacactac
     1081 ctgaaccgtc aaaaaacccg ttaccttaga gaccaccgcg gccgtcccgc ct
//
LOCUS       DAXHSJ010000245         1122 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000245 DAXHSJ010000000
VERSION     DAXHSJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.245"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(230..1084)
                     /locus_tag="ACFM8U_002044"
     CDS             complement(230..1084)
                     /locus_tag="ACFM8U_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6335214.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIAAFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWNVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          309 a          339 c          294 g          180 t
ORIGIN      
        1 cccgtcattc ccgcgcaggc ggaaatccgg acatccaacg ctgcagcaat ttgtcggaaa
       61 taaccgaaac tcaaaaaacc ggatgcccgc ctgcgcggga atgacgacgc aaagatggtc
      121 gttatttcgg ataaatatcc gtcttttcgt tccggatacc cgctttcgca ggaatggaga
      181 tggaaagatt tcagcctatt cggcaatcat caaataccgc ttcccaactt tatttttgat
      241 gcgacagccg gccggcaacc aaggtggcgg cgatgacgac aaacacgccc aaggcggaca
      301 cgggcgacag gtgttcgccc aaaatcaaaa ccgccagcag cacgccgacg acgggttcga
      361 gcgaaatcaa cagtcccgac acgttggcag gaacacggct catccccttg ttccacagcc
      421 aataggcgta ccagccgcac cccaagccca aatacagcag cgacaatacc atcccgacgt
      481 tccagtccac ggtataactt tgcgccaaag caagcgaaaa cggcaggcac atcaacgatg
      541 cggcggcaat ggaaacagat gtgaatgccg gtgcgccgat gcgtgcaatc agcctttgcg
      601 tcggacgcat agcggcacaa aagcccgcgc ccgccaacaa caccagcagg cagccgaacc
      661 agccgacttc gccgccctct tccgcaccgc ccgccatcag cagcgcgaca ccggcaaatg
      721 ccgccgcgcc gcatatccag tggtaggcac gcgctttgtc gttgaagaaa aagtgtccga
      781 caaacaccat cagcagcggc tcgagtccga caatgaccga tgcgctggcg gcggaagtgt
      841 atttcaaccc gacaaactga agcagcaggg tcagcacata gttgacaaac gccgcaatca
      901 gcaacggctt ccattcctca cgcggaatct tgccgacatg acggcggcag gcgggcagtg
      961 caggcagcgc ggcaattagc aggcgcacgc cgaccatcaa tgcgggatcg atgccgccat
     1021 agacatattt ggcggcaata aacgagctgc tccagataat cagggcaagg atttggtaaa
     1081 acatagtgag tgataaagtc agatggtcat acaaaagtat ag
//
LOCUS       DAXHSJ010000246         1118 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000246 DAXHSJ010000000
VERSION     DAXHSJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.246"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..759
                     /locus_tag="ACFM8U_002045"
     CDS             <1..759
                     /locus_tag="ACFM8U_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC6335215.1"
                     /translation="AGIAVIIANPRQTHQFAQSQPLTKTDAKDAKMPAFFTQMMTQKE
                     DWQTMPYQPPTEAEEVLEALVNRRNQSADMRTAEKNRLHQVHETQVGSVKQLIAHFDR
                     LIDELDKQIDNHTHTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASLVG
                     IAPHPRESGETKFKSRCFGGRSAVLKALYMATVAATRFEPLIRDFHQRPLSEGKPYKV
                     AVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
     gene            916..>1118
                     /locus_tag="ACFM8U_002046"
     CDS             916..>1118
                     /locus_tag="ACFM8U_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein GemA/Gp16 family protein"
                     /protein_id="HFC6335216.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKP"
BASE COUNT          292 a          268 c          303 g          255 t
ORIGIN      
        1 gcaggcatag ccgtgattat cgccaacccg cgtcagacgc atcagtttgc ccaatcgcag
       61 ccgctgacca aaaccgacgc caaagatgcc aaaatgcccg ccttcttcac acagatgatg
      121 acgcagaaag aagattggca aaccatgccc taccaaccgc ccaccgaagc ggaagaagtg
      181 ttggaagcct tggttaaccg ccgcaaccaa tcggcggata tgcggactgc cgagaaaaac
      241 cgtctgcatc aggttcatga aacgcaagtc ggaagcgtca aacaactgat tgcccatttt
      301 gaccggctga ttgacgaatt ggacaaacaa atcgacaacc acacccacac gcattttgac
      361 ggcaaagccc aagtggcaga gcaaatcaaa ggcatcggtt cgataacgac ggctacgctg
      421 atggcgatgt tgcctgagtt ggggcggctg tcgcacaaac ggatagcgag tctggtcggc
      481 attgccccac acccgaggga gagcggggaa accaaattca aaagccgctg ctttggcgga
      541 aggtctgcgg tgcttaaggc gctgtatatg gctaccgtgg cagcgacacg ttttgaaccg
      601 cttattcggg atttccacca acgcccgctg tccgagggta agccgtataa ggttgccgtt
      661 acggcatgta tgcgcaaact gctgacgata tcgaatgccc ggatgcgtga ttattttgcc
      721 gaaaacgata ccgccgaaaa cggtatccgg acggcttgat ttgagttttg gtatttttgc
      781 ccgacggggt gaaaaataca gttgcttttt tatgtctgtc cgtttcgcaa aaaacatcgg
      841 cttaatacta tatattgtat tttattggta taatatgcgt taattagtca atatattgtg
      901 ttttatgggt ttgagatgcg ccgggcgttg attgcgaaaa ttaagattgc tcaaaaggag
      961 ctgggcttgg acgacggtac ctatcgcgcg gtgttggagc gtgtgacggg caagcggtcg
     1021 tgtgcggata tggatgtttc cgaacttgag tctgttgtcg ctgatatgcg gtcgcacgga
     1081 tttaagccta aagcaaaagg taacccacac ggcaaacc
//
LOCUS       DAXHSJ010000247         1074 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000247 DAXHSJ010000000
VERSION     DAXHSJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.247"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(1..437)
                     /locus_tag="ACFM8U_002047"
                     /pseudo
     CDS             complement(1..437)
                     /locus_tag="ACFM8U_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(557..1015)
                     /locus_tag="ACFM8U_002048"
     CDS             complement(557..1015)
                     /locus_tag="ACFM8U_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335217.1"
                     /translation="MPSEHVFGRRFVGGYRSGTSEKGFQAVFGRVVTVENVIRLPEKF
                     GRFSGGLWYWAQQTQVQIRRCAVIVRMPIQPKQTSVFRKWMFDGAKVVRRMEGNLWVF
                     GMVALEKTCFKGQMPSENRFQTAFSVYLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          279 a          286 c          208 g          301 t
ORIGIN      
        1 ataaaaccgc tcacgtccaa cttgtcgccg ctgctcagac tatcggtata aacaatgctg
       61 tccggcatga ttttcttttt gatgacaggg agtaacgttt cagacttggc attatccacc
      121 acaacggtat agacccgtct gttgcgtttc agaatgccga aaacaaccac ttttcctgcc
      181 gcccgcgacc acgtctgcct ttacgccgtc cgccgaaatc gctttcgtcc agctcgacag
      241 ggctctcaaa aacctcatcg gcagccaaga ccaaatgata gttggtaacc gtgcggattt
      301 tacggtagaa cagtgctgcc gaattgggat ggatacccaa aatatcggcg gcagaacggg
      361 cggtaacttc cagtacaaaa aaaatggagc agttctttct gtactttttt ctttaatttg
      421 cagtgcgtta tcttcatatt tcgagggtaa catatctgct aatctagtac agcccctagt
      481 ttaaagcttt gtttcttaag tccgcagagt atgccatggt tagaccttca aagttgagta
      541 ttgtactatt ttgtttttaa ttaactataa aacgcttgtg tgaataggaa gactgcagaa
      601 tattgctgcc ttttcctgtt tgctttaaat aaacagaaaa tgccgtctga aaccgatttt
      661 cagacggcat ttgtccctta aaacacgttt tttcaagcgc gaccatacca aacacccaca
      721 ggttgccttc catccttcgt acaacctttg ctccatcaaa catccacttc ctaaatactg
      781 atgtctgctt aggttgaatc ggcattcgta cgattacggc acaccgccta atctgtacct
      841 gcgtctgttg cgcccaatac caaaggccac ctgaaaatct cccaaacttt tcaggtagcc
      901 ttatcacatt ttcgactgtc accacacgcc caaagaccgc ctgaaaccct ttttcagacg
      961 ttccgctccg atacccccca acaaaacgcc gcccgaacac atgttcagac ggcattttct
     1021 ttgcgtcgat tcattgcggt gtctacctgc tttaaggcgg acaaaggtgc ggaa
//
LOCUS       DAXHSJ010000248         1035 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000248 DAXHSJ010000000
VERSION     DAXHSJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.248"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(299..685)
                     /locus_tag="ACFM8U_002049"
     CDS             complement(299..685)
                     /locus_tag="ACFM8U_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC6335218.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(682..930)
                     /locus_tag="ACFM8U_002050"
     CDS             complement(682..930)
                     /locus_tag="ACFM8U_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFC6335219.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
BASE COUNT          283 a          246 c          182 g          324 t
ORIGIN      
        1 cagccatata tattaagtac ctgctcttta tgcgcttttt taggaaagga agatcaaacg
       61 gttcaatagc gaagtcttgc ccaaatttga tggacaggat atagaataca tgcgtgcata
      121 ccacaccctg ccaaagttgg gagatgcact taaggttgca cttgtggtcg ccaactatgc
      181 ctatacacgc ggatttcccg cgtttatagg ccgcgacgga agtctggctt ttaagcgaag
      241 acaataaccg aaaagaccgc ctcggcggtc tttcttgtgt acacagtttt tgtatttgtc
      301 agcttgatgc gttgacaact ctaattccat actgcggaat atattcatcg acagtcatca
      361 gttcaaagcc ttccgcttgg gtttgtgcaa tcaacatcct atcgaaaggg tctttgtgta
      421 tctccggaag gcttccagcc tgttttgcat gaaacagacc tataggcaac atttcaaaat
      481 cctcttcttg aagcacatca aaaaactctt ccggtaattt caacaacccc ttgttctgct
      541 tgatggaaat ttcccaaata cttgctgcac tgacaaagat cgcatttctc ggattttcta
      601 tcagtttgcg tgcagatatc cccagtttct tgtcatccaa caaccaccac agcaacgcat
      661 gggtatcaag cagaatcttt ctcacagagc cgactcctca aaaaataaag ctgccgtttc
      721 attgtcatcc tcaagaatac gtgaaatatc cgtattttcc atatgactga attttttcaa
      781 ccttcctgca tttcgtgccg gtttttcaat accgattagt tggacgcaag gcttacctgc
      841 cttcgcaata ataacaattt cccctgcttc tgctctttga atcaattgac tcaaattggt
      901 ttttgcctga tgaatatttg cttgaaacat aacacttctc atgattagct aacttaacta
      961 atatacatca ttaccaagat tttgggaatc tcattacata tatttgatta tatccgccgt
     1021 tttattcaca ccttg
//
LOCUS       DAXHSJ010000249         1034 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000249 DAXHSJ010000000
VERSION     DAXHSJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.249"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..227
                     /locus_tag="ACFM8U_002051"
     CDS             <1..227
                     /locus_tag="ACFM8U_002051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335220.1"
                     /translation="YRFLLIRYTTMNPSQYLFIDLNFDKIPDTRLCCFSIFQPTVNLK
                     QPVHAMLQFLFLSADCFDKELKIHFMHLKI"
     gene            335..895
                     /locus_tag="ACFM8U_002052"
     CDS             335..895
                     /locus_tag="ACFM8U_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HFC6335221.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          297 a          286 c          207 g          244 t
ORIGIN      
        1 cgtaccggtt tttgttaatc cgctatacca cgatgaatcc ttcgcaatat ctgtttatcg
       61 acctcaattt tgacaaaata ccggatacgc gcctttgttg cttttccatc ttccaaccaa
      121 ctgtaaatct caaacagccg gtacacgcca tgcttcagtt tcttttcctg tcggcggatt
      181 gtttcgacaa agaattgaaa atccatttca tgcaccttaa aatttaatct gcattcaaac
      241 cttttcactt tggaagcacc atttatcgga tgtcccttcg caataaacaa attttcccga
      301 taccgccgcc catttcaacc caaacccaaa acctatgaaa aacctcatcg ccttcaacaa
      361 accctatggc gttatctgcc aattttcacc gcacgaaaaa cacaaaagcc tcaaagactt
      421 tatcaatctt cccggcttct accccgccgg acggctcgac accgacagcg aggggctgct
      481 gctgctgacc gacgacggca ggcttcaggc acaaattacc gaccccaaat tcaaacaccc
      541 taaaacctac tgggcgcaac tggagggcgt acccgacgaa agccgattgg aaagcctaag
      601 aaaagggata gacttaggcg gtttcgttac ccgtccggca agcatccgca tcttgaaaca
      661 cggagaagca gattcgttat gggagcgcat cccgccgata cgcgtccgca aaaccgttcc
      721 cgatttttgg attgaaatta ccatttctga gggcaaaaac cgccaagtca ggcgaatgac
      781 cgccaaggcg ggctatccct gcctgcgtct gatcagagtg gcaagcggca gactgaaact
      841 gtttgatttg gatttaaaac ccggggaatg ggcatacgcc ccgtttaaac cataatcacg
      901 tttatctcat catttccaca aaagtgggaa tccggaattt tatagtggat taacaaaaat
      961 caggacaagg cgacgaagcc gcagacagta caaatagtac gaaaccgatt cactcggtgc
     1021 ttcagcacct taga
//
LOCUS       DAXHSJ010000250         1020 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000250 DAXHSJ010000000
VERSION     DAXHSJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.250"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(49..987)
                     /locus_tag="ACFM8U_002053"
     CDS             complement(49..987)
                     /locus_tag="ACFM8U_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335222.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT          188 a          287 c          321 g          224 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat aggtcgtctg aaaacgggct aacggctgcc
       61 cagcggcaat ccgatgtccg aaatcagcct gtcgggcagg acgtcgtcgc ggaagcggca
      121 aagctgcgcg ggacggcctt tgctgcccga tacgccggta tccgacggct cgataaggtt
      181 ttgctgctga atctggcggc ggaagttttg cttgtgcagc aatcttccgc tgatggcttc
      241 gacactgttt tgcagttgca gcagcgtgaa ttcgggcggc atcagttcaa aaatcacggg
      301 gcggtatttg attttggcgc gcaggcggga cagggcggtc gccagtacgc gacggtgatc
      361 gtggcgcatg ggctgccccg tgagcgcgaa gtcgaagttt gcctgcggct cggcggcttc
      421 cactatcaga ccgctttcat acagcatttc atagcgttgt aaaacgtatt cttccgacca
      481 gttttccggt tcgacccccc agcacaaatg aatgcgcttg agccgcattt gacgcacttc
      541 ctccgtgtcc gccgaatttg cccaaatgcg caggcggctg acgacggcgt cgcgctgccc
      601 gccgtcggtg cgcaagtctt cccacgggaa atagccgtag cagtcctgcc atttcgcatc
      661 cgggtgcagg atgctgtcgg ctgcctcgcg caccagcccc aaatagctga cgtacaagac
      721 gggcatgccg tgttcgttgc ggcggtgggt atcgacaaag gtgtaaagct gttccacata
      781 gcccataggc tgcgaagtct gcttggcaac ccacagcttc acgcccgcct gcagggaatt
      841 gcgcagaggg gagagcgggc cgttgggcag gagtgtgcct tgggcgacgg ttaatacccg
      901 caggccgccg tcggtaacgg caatcaatac gggaaccagc tcgacgatgc tttgcggcgg
      961 ggcttcggct tcggggtagg catccatgct cggctttctg cgggtgggaa aggctttatt
//
LOCUS       DAXHSJ010000251          997 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000251 DAXHSJ010000000
VERSION     DAXHSJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.251"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..>997
                     /locus_tag="ACFM8U_002054"
     CDS             <1..>997
                     /locus_tag="ACFM8U_002054"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6335223.1"
                     /translation="RQSGLDIYAWHGMYFFPRFPFIDIDRIRWVRVGLKAFKHYIREN
                     GLPDLIHAHRMNYAGILAQKISQKYGIPYVVTKHSSTITRGLIRPHQWQPMKNAAAHA
                     AARLAISRHFAHVLQHKYGCEWQYLPNILGGIFNRPFEQREKNNKPHFVFCTVSHLRR
                     LKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQRLKQQAADLGITHAVTFLGALQPEA
                     VLDLMRNSDAFVLASRTETFGVVYIEALSQGLPVIATRCGGAESIVSDGNGYLVPVDD
                     DDALADALIKMYEHHSDFEPARLRENCLNEFGENAVIGRLIGIFRQAIAEYGKKIP"
BASE COUNT          250 a          301 c          241 g          205 t
ORIGIN      
        1 cggcaaagcg gtttggacat ctatgcatgg cacggcatgt atttcttccc ccgctttccg
       61 ttcatcgaca tcgaccgcat ccgctgggtg cgcgtgggtt tgaaagcctt caaacactac
      121 atccgcgaaa acgggctgcc cgacctgatt cacgcccacc gcatgaacta tgccggcata
      181 ctcgcccaaa aaatttccca aaaatacggc atcccctatg tcgtcacgaa acacagcagc
      241 accatcacgc gcggtctgat ccgtccccac caatggcagc ctatgaaaaa tgcggcggcg
      301 cacgccgccg cacgtctcgc catcagccgc catttcgcac acgtcctgca acacaaatac
      361 ggctgcgaat ggcagtacct ccccaacata ctgggcggaa tattcaaccg accgtttgaa
      421 caaagagaaa aaaacaacaa accgcatttc gtgttctgca ccgtctcgca ccttcgccgt
      481 ctcaaaggac acgatgtcct gctcactgcc tttgcccggg cgttggcaca atgcccgcaa
      541 ctgcgtctaa acatcggcgg cagcggacag gaagaacagc ggctgaaaca gcaggcggca
      601 gacttgggca ttacccatgc cgttacattt ttgggcgcat tgcagcccga agcagtcttg
      661 gatttgatga ggaacagcga cgcattcgtc cttgccagcc gcaccgaaac cttcggcgtg
      721 gtctatatcg aagcactgtc ccaaggattg cccgtcattg caacacgctg cggcggtgcg
      781 gaatctattg tttcagacgg caacggatat ttggttcctg ttgacgacga cgatgccctt
      841 gccgacgcac tcatcaaaat gtatgaacac cactctgatt ttgaacctgc ccgacttagg
      901 gaaaactgtc tgaacgaatt tggcgaaaat gccgttatag gcaggttgat cggcattttc
      961 cgacaggcaa tcgcagaata cggtaagaaa ataccgg
//
LOCUS       DAXHSJ010000252          990 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000252 DAXHSJ010000000
VERSION     DAXHSJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.252"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..980)
                     /gene="mafB"
                     /locus_tag="ACFM8U_002055"
     CDS             complement(<1..980)
                     /gene="mafB"
                     /locus_tag="ACFM8U_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6335224.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKKRVYAVQTFDATAVSPVLPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKINTVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWENDPNQNW
                     WGNRMDDIRGIIQGAANPFLTGFQGLGVGAITDSAVNPVTYAAAQQTLQGIHDLGNVS
                     PAAQLAAATALQDSAFAVKDSINSAKQWADAHPNITATAQTALAVAEAAGT"
BASE COUNT          233 a          249 c          232 g          276 t
ORIGIN      
        1 gtacctgcag cctctgctac ggcaagggca gtttgggcag tagctgtaat gttcggatgg
       61 gcatcagccc attgcttggc ggaattgata ctgtccttaa ccgcaaaagc actgtcttgt
      121 aatgcggttg cagccgcaag ttgtgctgcg ggacttacat ttcctaaatc gtgaataccc
      181 tgtaaagttt gctgtgctgc cgcataggtt accgggttta ccgcgctgtc tgtaatcgcc
      241 ccaactccca atccctgaaa acccgttaga aaaggattgg ctgcaccttg gatgatgccg
      301 cgaatatcat ccatacggtt accccaccaa ttttgattgg ggtcgttttc ccatatcagt
      361 tttcctgctt catccgcacg gctgaaaaaa ccagaggcgg caccggcatt ttcttttagc
      421 cagcggtctg aaaaaggggc ttgcggaaca gtgtttatct ttgtttttgt agaagttcct
      481 ttgacataat agctgtatat atcccttgct cctccggggg gcggataatc gctgccttgc
      541 ggcccgtcat atccatcctc cggatggatt tccgaccctg tccgatgaag ttggtaaaca
      601 gtaaaaccac cgtctacacc gccgctgaaa tcagaagtgc tttttgaatc atgatgatcg
      661 aacggactgt gtacttcatg tccgtgccct gaaaaatggg tttcataacc gataatgcct
      721 tcaaaccctg tccgttcgtg tgtaataggc agtacaggac tgaccgcagt tgcatcaaat
      781 gtctggacgg cgtaaacccg ctttttaaca ctgccgcgag cattaccaaa cagatggtat
      841 ttaccacccg gttcgtagtg ttttgcctgc atatcatcgc gcaaacgggc atccaaaccg
      901 ttcgcatgac taatggggat ttgcagcaac gatattgctg ctgcaaacag catcatgaat
      961 ttttgaatag gcaaattcat tttgttttcc
//
LOCUS       DAXHSJ010000253          983 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000253 DAXHSJ010000000
VERSION     DAXHSJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.253"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..>983
                     /gene="pilC"
                     /locus_tag="ACFM8U_002056"
     CDS             <1..>983
                     /gene="pilC"
                     /locus_tag="ACFM8U_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6335225.1"
                     /translation="QAQTQTHKYAIVMNGQQLPEVKWGNSYNSLTVKSNEREVTHTSN
                     FVVAKKHISFSFNNTDEVVAEKKDAVVFGAATYLPPYGKVSGFDTAKLVERKNAVDQI
                     GTTHPGLIGYSYQDVTCASGNCPELSYRTQFTFNNGLKRNGNGGRLDIYEDKSRDNSP
                     IYKLKDHPWLGVSFNLGGESSFKPKRQGSLVSSFREDVTQQNGTQDQYKSKNLVYTTD
                     DYNSKGNKNHQDKHHAVAFYLNAKLHLLDKKQIQHIAQGVTVDLGTLKTRIEPTEAWK
                     KQRYNFFNGGTWTYEDKGSVSVKLKLPEVKAGRCINKQNPNPNAKALSPALT"
BASE COUNT          323 a          225 c          219 g          216 t
ORIGIN      
        1 caagcgcaaa cgcaaacgca taaatacgct attgtaatga acggacaaca gctgcctgag
       61 gtaaagtggg ggaattcata taactcattg acggtcaaaa gcaatgaacg cgaagttacc
      121 catacgagta attttgttgt agcaaaaaag cacattagtt tctcattcaa taataccgat
      181 gaagttgttg ctgaaaaaaa agatgctgtc gttttcggcg cggcgaccta cctgccgccc
      241 tacggcaagg tttccggttt tgataccgct aagctggtcg agcgcaaaaa tgccgttgat
      301 cagattggta cgacccaccc tgggttgata ggctacagct accaagatgt cacttgcgcc
      361 agcggaaact gtcctgaact tagctataga acccaattta ccttcaataa cgggttgaaa
      421 agaaatggaa acggcggcag gctggatata tacgaagaca aaagccgcga caattcgccc
      481 atttacaaat tgaaggatca tccatggttg ggcgtgtctt tcaatttggg cggcgagagc
      541 tccttcaaac caaagagaca aggttctttg gtatcttctt ttagagagga tgtgacgcag
      601 caaaatggta cacaagacca atacaaaagc aaaaacctcg tttatacgac agacgattac
      661 aatagtaagg gtaataaaaa ccatcaggac aaacaccacg ccgtcgcctt ttatctgaac
      721 gccaaactgc acttgctgga taaaaaacaa atccaacata tcgcgcaagg cgtaacagtt
      781 gatttgggta ccttgaaaac acgcatcgag ccgacggaag cgtggaaaaa acagagatat
      841 aactttttta atggtggtac ttggacgtat gaagataaag gatcagtcag cgtcaaactc
      901 aaattgccgg aagtcaaagc aggtcgctgc atcaacaaac agaaccccaa tcccaacgct
      961 aaagcccttt cccccgccct gac
//
LOCUS       DAXHSJ010000254          959 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000254 DAXHSJ010000000
VERSION     DAXHSJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.254"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(137..769)
                     /gene="mtrR"
                     /locus_tag="ACFM8U_002057"
     CDS             complement(137..769)
                     /gene="mtrR"
                     /locus_tag="ACFM8U_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC6335226.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          220 a          209 c          249 g          281 t
ORIGIN      
        1 agattgagca taaaatttta gtaacctatg ttattgcaaa ggtctcaata tgtattttta
       61 gaagaaggct ttgtgtcatt ttgatgccgt ctgaaagggt ttgttcgttt cagacggcat
      121 tgctaccaag gcttgattat ttccggcgca ggtcgggatg gttttccaag ttgtccatca
      181 ttatcccgat gatgcgcggg gcggttttgc ccaaatcgaa acgttcgcag gaagagaacc
      241 accgccaaat cagcccgtcc aaggttgatt tgatgaagat aactgccgtt tccttgtcca
      301 aatcgtcagc caaatcctga ttttccaccg cttcggtcaa aacggcggta attttctcgc
      361 gccagattgc ctgatgcttg cgggcaatgg cgataacggc ggcgttttgc tccgtgtgtt
      421 cgcattttaa aaacaggatg ttgtggaatt tgtagtagat gtcgttgctt tgcagccgct
      481 cgaaaaagtg cagcagcgtg tggcggaata ccgcccaaga ccctccttcg gcatcttcgg
      541 catcttgcgc gatgcagttt tcgatgtcgt cgcagatacg ttggaacagc gcgtcgaaca
      601 agtcttcctt atttttgaaa tgccaataga gcgcgccgcg cgttacgccg gcggcttggg
      661 cgatttcgtt gagcgaggtg cgcgcaatcc ctttgcggta aaaggtttcc aaggcggcaa
      721 gcatcaggtg ttctttggtt tttaaggctt cggttttggt ttttctcata atggcggttt
      781 cgtttcgggt cggtttgatg agggcggatt ataaaaaaga ctttgtaacc atgcaatcgt
      841 gtatgtataa tgaaacccat gaaattgaga ctacatctca actttgaaaa cccatgaaac
      901 ctgcttcgca acccgtttga acatcgggtt ggcgaagcag gcggttttta tatcctgaa
//
LOCUS       DAXHSJ010000255          943 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000255 DAXHSJ010000000
VERSION     DAXHSJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.255"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(92..871)
                     /locus_tag="ACFM8U_002058"
     CDS             complement(92..871)
                     /locus_tag="ACFM8U_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC6335227.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWAV"
BASE COUNT          229 a          299 c          191 g          224 t
ORIGIN      
        1 tctttgagct aaggcgagcc aacgccgtac tggtttaaat ttagttcact ataccgatac
       61 atccgaatat gccagaccgt ctaaacggta ttcataccgc ccaatcctgc acgcgcttca
      121 aatcgttttg cgccaaagta tccgcgtggc ggttacggct ctgatattcc ctgtctttca
      181 tgatgctgct tgccacataa ttcaaatgtg cctttgccgc ctccgaagcc tcgcccggcc
      241 gacggttcga tatcgcctca tacagcacgc ggtgctgcgc catcagtttc ggacgcggat
      301 cttcttcctg attcagataa ataaggctgc tgcgcgtctg ccggtacagc attttcaaca
      361 aaccgcccga caaatggctg aacaacaaat tgtgcgccgc atccgcaatc gtctgatgga
      421 agctgacatc ggcttcgctc tgatgttcca gattgccgct ttcgcacgcc tcctcaaact
      481 tttcaagcca aaacccaatc cgcttcaaat cggcatccgt ccggcgttct gccgccaatg
      541 ccgccataca gccctcgatg tggcagctga aatcaaaaac atcctgttcc caattggaat
      601 gcttgcccaa aagctcctgc caactttgca aaaaatcctg ctgcggcttg accgaaacat
      661 aataaccgtc tccctgcctc gcttccaaaa cctgacgggc gaccaaaaca ttcaatgccg
      721 acctgaccga cgggcgcgaa acgccgaact cttccgccaa aacgcgttcg ggcggaatct
      781 tgcccccttc cgcgtaaacc ccttccacga tgcggccttc caataccgac aatacctgat
      841 cgctgatttt ctgaggcctt accagtttca tcactcctcc tttataaaga ttccctgcag
      901 aacccttccg aaatatagtg gattaacaaa aatcaggaca agg
//
LOCUS       DAXHSJ010000256          905 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000256 DAXHSJ010000000
VERSION     DAXHSJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.256"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..215
                     /locus_tag="ACFM8U_002059"
     CDS             <1..215
                     /locus_tag="ACFM8U_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335228.1"
                     /translation="DAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATLLALDWQGN
                     KPLGAEELADLKPLYKDLKNNIGNIV"
     gene            546..>905
                     /locus_tag="ACFM8U_002060"
     CDS             546..>905
                     /locus_tag="ACFM8U_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC6335229.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQISEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT          281 a          146 c          212 g          266 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggca aaaaaacaca tgcgaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg aggagctggc ggatttgaaa ccgctttaca
      181 aagacttaaa gaataatatt ggaaatattg tatgaacaaa aaattaaact atatttttat
      241 gttggactgt ttagggttgg tgatattgtt tacttgtata atagctactt ttgaaagaga
      301 ttatggattt aaaattttta ctaattctaa gaggcctgaa ttttattatt ggattggaat
      361 gttttattat ggaattattt cttgctggtt tgattatcaa ttaatttcaa caaaggcgaa
      421 ttcgtataaa agaaaagtta aacaatataa aattttttca gtaatatttt cagttttgat
      481 atttatttct actatagtaa aactttaaat tttggagcaa aaatttatga gcgattcaat
      541 tgaatatgta ttgggaacgc ggtctgcaca tgtataaggc aagtgccgtc gtgccgacgg
      601 gatatgtacg ggttgggaat accgcgccgc tggtcggcga agacacgcaa cggtatgcct
      661 ctttttgggg cgacggctac gacgtgtacc gtcagttgag atggcagcag atatccgaaa
      721 aacagagaaa ggcattcaaa aaagccgcta aaagcaaaaa gaccgtgatg tttgccggac
      781 gggaatacgg catatccaaa cagaatttga gcgatgtttg ggatgatttt gaagacgcga
      841 tggaactgaa ggcgtttccc tgcctgtctt cgctgtttct gaccaaatgg cataaaaatc
      901 tatat
//
LOCUS       DAXHSJ010000257          891 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000257 DAXHSJ010000000
VERSION     DAXHSJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.257"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT          218 a          218 c          218 g          237 t
ORIGIN      
        1 gcgaggcaac gccgtactgg tttttgttaa tccactatac cgtcgttccc acggtcagga
       61 tttcagattg cggacatctg tcagaaaaga caaaaaacct tccgccgtca ttccctacag
      121 gcgagaatcc gatccgttga aattcggttg ttttaaataa attcttacag ctttgatttt
      181 ctgtttttcc gataacgccg taactttgaa acgcgaaagc ggtaatccga tccgttggga
      241 ttttgcaact tcaaatcaat ccgcaaactg aaatcccgtc attcccgcgc agtcgtgaat
      301 ccgaacgcgt ccgcacgaaa acctgcatcc cgtcattccc acggaagtgg gaatctagga
      361 cgtaaaatct caagaaaccg ttttacccga taagtttccg caccgacaga cctggattcc
      421 cgcctgcgcg ggaatgacga aatttcagcg agccgtaggg tgggctgtag ggtgggcttc
      481 agcccaccaa tttcaccgca tcaaaggttt ttgggaatac ggcggtttcg gttttcggtg
      541 ggaatgggcg gaatggataa cattgacgga gttgggggaa taggtggaaa cggtgggatt
      601 ggtgggctga agcccaccct acagccagcc ctatgtccac gctgcacatt ctacgcccgc
      661 cttgcgtttc ttataagcag cattttacgc aatactttgc gtccgctgaa aaaatttcaa
      721 acggtctttt ttatcaaatg cgaaaaatat aacgtcattc ccgcgcaggc gggaatctag
      781 tctgtcggtg cggaaactta tcggataaaa cggtttcttt agattttacg ccctagattc
      841 ccgcctgcgc gggaatgacg ggatttgagg tttctgtttt tgattttccg c
//
LOCUS       DAXHSJ010000258          888 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000258 DAXHSJ010000000
VERSION     DAXHSJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.258"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..>888
                     /locus_tag="ACFM8U_002061"
     CDS             <1..>888
                     /locus_tag="ACFM8U_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335230.1"
                     /translation="RGDTYGGWRYLANLGDRYADDAAAIVGKDTNLNGLNLWMKKGVE
                     NLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGRLPNTSEIERSYYKAVTENGVS
                     SSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQAVNNPNGSEWDNRKQLISALDK
                     GFDGSFKEKPFTFLQSVIMDVTKLSVEYTIDGWQKIGGWGNGIINDLYKSVAKREWTG
                     IFEIVNNNIKQFRDLFPNPEGWIDDGHQCLAPWVKETKKRNGKYHVYDPLALDLDGDG
                     IETVAAKGFSGSLFDHTNNG"
BASE COUNT          286 a          134 c          215 g          253 t
ORIGIN      
        1 ccgtggcgat acctatggcg gttggcgtta tttggctaat ttgggtgacc gttatgcgga
       61 tgatgctgcc gcaattgtcg gtaaggatac aaacttaaat ggtttgaatt tatggatgaa
      121 aaaaggtgtg gaaaacctat gggatgatac ggtcggtaaa aagacccgtt tagagaaatt
      181 tgatcgggtt gcactgcaac atttcagcca atatgtagat ctaattaatg aaaataatgg
      241 tagattacct aacactagtg aaattgagag aagttactat aaagccgtta ccgaaaatgg
      301 tgtttcttct agtgcagcta ttgatttagt tattaatcgt tcacttccgg atatggcgga
      361 tggttattgg gcattaggtt tggggataga agccgaacgt atccacaatg agcaagcagt
      421 aaataatccg aacggtagcg aatgggataa tagaaagcag ttaatatctg ctttagataa
      481 aggatttgat ggatctttta aagagaagcc ttttactttt ttacaatctg tgataatgga
      541 tgtaacaaag ttaagtgttg aatatacaat agatggttgg caaaaaattg gaggttgggg
      601 taatgggata atcaatgatt tatataaaag tgttgcaaaa agagagtgga ctggaatatt
      661 tgagatcgtt aataataaca tcaagcaatt tagagatctg ttcccaaatc cggaaggctg
      721 gatcgatgat ggtcaccaat gtttagctcc ttgggttaaa gaaactaaaa aacgcaatgg
      781 caaatatcat gtctacgacc cccttgccct agatttggac ggcgacggca tagaaactgt
      841 cgccgccaaa ggcttttcag gcagcttatt tgatcacacc aacaacgg
//
LOCUS       DAXHSJ010000259          830 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000259 DAXHSJ010000000
VERSION     DAXHSJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.259"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..639
                     /locus_tag="ACFM8U_002062"
     CDS             <1..639
                     /locus_tag="ACFM8U_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6335231.1"
                     /translation="DYPQATGANNTSTVSDYFRNIRAHSIHPRVSVGYDFGGWRIAAD
                     YARYRKWNNNKYSVNTKNVQVHKSNGNREDLKTENQENGTFHAVSSLGLSAVYDFDTG
                     SRFKPYAGVRVAYGHVRHSIDSTKKTTNVLTVPSNIPGGTPTIYNQGSTQNAYHESHS
                     IRRLGLGVVAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYRF"
BASE COUNT          229 a          250 c          185 g          166 t
ORIGIN      
        1 gattatccgc aagcaaccgg tgcaaacaac acaagcacag taagcgatta tttcagaaac
       61 atccgtgcgc attccatcca ccctcgtgtg tcggtcggct acgatttcgg cggctggagg
      121 atagcggcag attatgcccg ttacagaaaa tggaacaaca ataaatattc cgtaaacaca
      181 aaaaatgtgc aggtgcataa aagcaatggc aacagggaag acctgaagac ggaaaatcag
      241 gaaaacggta cattccacgc cgtctcctcg ctcggcttgt ccgccgttta cgatttcgat
      301 accggttccc gcttcaaacc ctatgcaggc gtgcgcgtcg cctacggaca cgttagacac
      361 agcatcgatt cgaccaaaaa aacaacaaat gttcttaccg tccccagcaa tattcctggc
      421 ggaacaccta cgatttataa tcagggaagt acgcaaaacg cctatcacga aagccacagc
      481 atccgccgct taggtcttgg tgtcgtcgcc ggtgtcggtt tcgacatcac gcccaagctg
      541 actttagaca ccggataccg ctaccacaac tggggacgct tggaaaacac ccgcttcaaa
      601 acccacgaag tctcattggg catgcgctac cgcttctgat tccccgatac cgatgccgtc
      661 tgaaccttca gacggcattt ttgattcacc tgccgtttac agacgcgggg cgggcgtgga
      721 aatacccaaa ccgtcattcc cgacaacacc gcaatcttga aacccgccat tcccgataac
      781 agcgcaatct tgaaacccgt cattcccgcg caggcgggaa tccagccccc
//
LOCUS       DAXHSJ010000260          811 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000260 DAXHSJ010000000
VERSION     DAXHSJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.260"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..>811
                     /locus_tag="ACFM8U_002063"
     CDS             <1..>811
                     /locus_tag="ACFM8U_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6335232.1"
                     /translation="AKNVDWKQVQLAYDKWDYKQEGLTGAGAAIVVIIVTALTYGYGA
                     AAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASLYSQAAVAIINNKGDVGK
                     ALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSSTGNQTIANLGGRL
                     ATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASKIKTTFSDDYVAKQ
                     FAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATLSDAEKHKV"
BASE COUNT          238 a          194 c          218 g          161 t
ORIGIN      
        1 cggccaagaa cgtcgattgg aaacaggtgc agctggccta cgacaaatgg gactataaac
       61 aggaaggctt gaccggagcc ggtgcagcga ttgtggtgat tattgtaacc gctctaactt
      121 atggatacgg agcggctgca gcgggtagcg taactgccgc aggaagtagt acagccgcag
      181 ctgcaacaac gacagcggca gcaactacca ctgtttctac tgcagctgcc atgcaaaccg
      241 cagctttagc ctccttgtat agccaagcag ctgtagccat catcaataat aaaggcgatg
      301 taggcaaagc attaaaagat ctcggcacca gtgatacggt caagcagatt gttacttccg
      361 cactgacggc gggtgcatta aaccagatgg gagcagatat tgcccaattg aacagcaagg
      421 taagaaccga actgttcagc agtacgggca atcaaaccat tgccaacctt ggaggcagat
      481 tggccaccaa cctcagcaat gcaggtatct cagctggtat caataccgcc gttaacggcg
      541 gcagcttgaa agacaactta ggcaatgccg cattgggagc attggttaat agcttccaag
      601 gggaagccgc cagcaaaatc aaaacaacct tcagcgacga ttatgttgcc aaacagttcg
      661 cccacgcttt ggcgggttgt gttagcggac tggtacaggg aaaatgtaaa gatggggcaa
      721 ttggcgcagc agttggggaa atcgtagctg aatccatact tggcggcaga aaccctgcta
      781 cactcagcga tgcagaaaag cataaagtta t
//
LOCUS       DAXHSJ010000261          726 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000261 DAXHSJ010000000
VERSION     DAXHSJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.261"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(44..610)
                     /locus_tag="ACFM8U_002064"
     CDS             complement(44..610)
                     /locus_tag="ACFM8U_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HFC6335233.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRVLGGLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          206 a          249 c          143 g          128 t
ORIGIN      
        1 caacgacgta ctggtttttg ttaatccact atattttccg acatcattga atcaaaccca
       61 aatgcgacaa gagcgtccat gtgccgatgg caatcaacac caaacctccg gcaaattccg
      121 cacgcctgcc gaacaatccg cccaatactc gccccgccgt cagcccgacc gccaccatca
      181 ccgtcgtcgc cataccgatg attgcggcgg caaaggcgat gtttacctcc ataaacgcca
      241 agcccacccc gactatcatg gaatcaatac tggttccaaa agcagtcaaa accgtcatcc
      301 ataggctttc ccgtttgctt tcgcgcacat cttccgcctt gccggacagc ccttcgcgca
      361 tcattttcag acccagcccg cccagcagga cgaaagccgc ccaatggtcc cattcgctga
      421 taaacggctt ggcataaaaa ccgcctaccc agcctgccag cggcgtgagc gcttcaaccg
      481 tgccgaacac caaagccgtt gctgcaattt tgcgcggggg catcctgacc gccgcaccct
      541 ttgccaatgc gacggcaaac gcatccatcg acatccccag cgcaatcaag agcaaagcgt
      601 aaagactcat actgcacccg tcctcaaaaa gggcggatta tagcaaaaca gcaaagcgcg
      661 aaaaacgccg cacgaaaacc cacatcccgt cattcctgca aaaacaaaaa atcaaaaaca
      721 gaaatc
//
LOCUS       DAXHSJ010000262          717 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000262 DAXHSJ010000000
VERSION     DAXHSJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.262"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            98..478
                     /locus_tag="ACFM8U_002065"
                     /pseudo
     CDS             98..478
                     /locus_tag="ACFM8U_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218444.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          197 a          196 c          173 g          151 t
ORIGIN      
        1 acgaaagtgg gaatccagaa cgtaaaatct caagaaaccg ttttcccgat aagtttccgc
       61 accgacgagg ctggattccc gcctgcgcgg gaatgacgaa tccattcttt tggctgaagg
      121 tcaaaaatca gccgtcaccg agtattacct gaatcacggc gaatggcccg gcgacaacag
      181 ttctgccggc gtggcatcct cctctgaaat caaaggcaaa tatgttgaga aagttgaagt
      241 cgcaaaaggc gtcattaccg ccacaatgct ttcaaccggc gtaaacaaag aaatccaagg
      301 caaaaaactc tccctgtggg ccaagcgtca agacggttcg gtaaaatggt tctgcggaca
      361 gccggttacg cgcgccggca ccggcaccga cgacgccgtc aacaaagcca ccggcaacaa
      421 cgaaatcgac accaagcacc tgccgtcaac ctgccgcgac gcttcatctg ccggttaagg
      481 caaattatac cataaatttt aaataaatca agcggtaaat gattttccac ggccactcgg
      541 attaatccgg gtggcttcct ttttaaaggt ttgcaaggca agcggggtcg tccgttttgg
      601 tggaaataat atatcgattg cgcttcaagg ctctgcgcgt acctcattgc cgcccgttta
      661 aacacggggt ttatctgaca ggcgcgcaat ccgccctctc atttaccgaa caagcgg
//
LOCUS       DAXHSJ010000263          704 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000263 DAXHSJ010000000
VERSION     DAXHSJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.263"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            52..>704
                     /locus_tag="ACFM8U_002066"
     CDS             52..>704
                     /locus_tag="ACFM8U_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6335234.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGEQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARA"
BASE COUNT          226 a          203 c          157 g          118 t
ORIGIN      
        1 ttgttatgca agtaaatatt cagattccct atatactgcc cagacgcgtg cgtgctgaag
       61 acacctccta cgcttgctgc agaactttcg ggtaaaaccg gtgtgagcat tagcgcaccg
      121 tatgccaatg agaacagtcg catcctgctc agcaccacgg atatcagttc ggaaaacggc
      181 aaaatcaaaa ttcaatctta cggtgaacaa tattactatg cgagacagag cgaactctat
      241 acctttgaac gccgcagcta caaaaccggc aaatggtaca accgcaaaca cattaccgaa
      301 gtcaaagaac acaaaaacgc caagcccgac gcagtaaacc tcagcgcatc ccaaggcatc
      361 gacatcaaat ctggtggcag catcgacgcc tacgccaccg cattcgatgc ccccaaaggc
      421 agcattaaca tcgaagccgg gcggaaattg acactctatg ccgtagaaga gctcaactac
      481 gacaaactag acagccaaaa aaggcgcaga tttctcggca tcagctacag caaagcacac
      541 gacaccacca cccaagtcat gaaaaccgcg ctgccctcaa gggtagttgc agaatcagcc
      601 aacctccaat cgggctggga taccaaactg caaggcacac agtttgaaac cacactgggt
      661 ggcgcaacca tacgcgcagg cgtaggtgag caggcacggg caga
//
LOCUS       DAXHSJ010000264          692 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000264 DAXHSJ010000000
VERSION     DAXHSJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.264"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..336)
                     /locus_tag="ACFM8U_002067"
     CDS             complement(<1..336)
                     /locus_tag="ACFM8U_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6335235.1"
                     /translation="MYALSEKYNDNGFKAYKVLGEGGGIHTEYNYKFDKSLNLNVLES
                     STGARSLEKVPVKVTASVSRAAVLSGVGKLARLGAKLSTRAVPYVGTALLAHDVYETF
                     KEDIQAQGYQ"
     gene            complement(377..667)
                     /locus_tag="ACFM8U_002068"
     CDS             complement(377..667)
                     /locus_tag="ACFM8U_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335236.1"
                     /translation="MNKNERDFFYISNSDLDKLSESYPDRPLSYVFYCYLKETGLLKN
                     FSMDKCHNFFNRINFNESCFEIKFKDDSFFIIGNGKIDVSDSNNFFSVSFEC"
BASE COUNT          232 a          126 c          104 g          230 t
ORIGIN      
        1 ttggtagcct tgtgcctgta tgtcttcttt gaaagtttcg tatacgtcat gggctaaaag
       61 ggctgttccg acataaggaa ctgcccttgt gcttaatttc gcgcctaagc gggcaagttt
      121 gccgactcct gacaagacgg cggcgcggga aactgatgca gttactttaa cggggacttt
      181 ttcaagagag cgtgcgcctg ttgaactttc taatacattc aaattcaaac ttttatcaaa
      241 tttgtaatta tattctgtat gaattcctcc tccctcacct aaaactttgt atgccttaaa
      301 tccattatcg ttatattttt ccgaaagtgc atacatcaat ttcccatttt taatttctaa
      361 atctgctgaa aaagatttag cactcaaaag aaacagaaaa gaaattatta gaatccgaaa
      421 catcaatttt tccattgcca ataatgaaaa atgaatcatc cttgaattta atttcaaagc
      481 aagattcatt aaaattaatt ctattaaaaa aattatgaca tttatccatt gagaaatttt
      541 tcaatagacc agtttctttc aaataacaat aaaacacata agaaagaggc ctatcaggat
      601 aagattctga caatttatct aaatcagaat ttgatatata gaaaaagtca cgttcatttt
      661 tattcatggt ttcaagcctc aaatgtctaa tt
//
LOCUS       DAXHSJ010000265          680 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000265 DAXHSJ010000000
VERSION     DAXHSJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.265"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..354)
                     /locus_tag="ACFM8U_002069"
     CDS             complement(<1..354)
                     /locus_tag="ACFM8U_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6335237.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGVGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQAQGYQ"
     gene            complement(338..655)
                     /locus_tag="ACFM8U_002070"
                     /pseudo
     CDS             complement(338..655)
                     /locus_tag="ACFM8U_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
BASE COUNT          189 a          139 c          124 g          228 t
ORIGIN      
        1 ttggtagcct tgtgcctgta tgtcttcttt gaaagtttcg tatacgtcgt gggctaaaag
       61 ggctgttccg acatagggaa ccgcccttgt gctgaatttc gcgcctaagc gggcaagttt
      121 gccgaccccc gccaatacgc cggcgcggga aatgctggcg gttattttgg cgttgattcg
      181 ggcttttgcg cccgtaggga tgtgtgttaa atctaccgtt tttattaaat cagatgaata
      241 agttttacta tttttaggta caaacttatg aattttcgca ccttgtccgg tatcaactga
      301 aacagtttca gatattttta ctgcatttgc attcgcttca aacgaataca tcatcaaaat
      361 tgcaattatc gacaatttcg caaaattcaa atttgtatat tttatgacca tctttccgag
      421 attctttaat taccatttct caattatcag aaaatgagat tagccaaata tcatgtttaa
      481 ttcttctatt ccagaaaaaa gagaaacaat caataacatt ttcagactta ttaatcttcg
      541 caaattcaac aaattcagat tgcgctataa ccgccatcga ttgcccaaaa tacttgctag
      601 acagctgata tttataaagt gccaactgcg cctgcgtaat aaacggcttg ttcatggttc
      661 tgcctttcaa tgattgtttt
//
LOCUS       DAXHSJ010000266          676 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000266 DAXHSJ010000000
VERSION     DAXHSJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.266"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(91..642)
                     /locus_tag="ACFM8U_002071"
     CDS             complement(91..642)
                     /locus_tag="ACFM8U_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335238.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          187 a          270 c          126 g           93 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc ccaagctgcg gctgccaaac acatcggctg
       61 cttctcgacg cacccgctga aaaatgcgtc ctatccgtcc gccttgcccg caccccctga
      121 ctttgccgcc ctgcccgatg ccgtctgaag cctgaaccgc cccaccagcc cgatgacgca
      181 cgacaccgcc ggaaagggca tacacaaagc cgtcagcaca taacgcgcat tccccgtatt
      241 gaccacgtcc gcgcccttca gcagcagcgg tagcgcgttc gcccactccc acaccgacgg
      301 cgcgctgaac gccaataccg ccaccagccc caacatccgg aacaccccga tttccaacac
      361 ctgccgccgc cgcaccgaac ggttgagcaa aaacacaacc gccaacccga aacagaccac
      421 cgccgccata ccgccgttgg cgacaatctg caaactgttg aatgcaatat cgggcgtaac
      481 cgccaacacg ccttccgaat ccggcacggc acgctgcaaa tccaaaccct gcaacacatt
      541 caacacaaaa ccgtagatca aatcaaaaaa caccacgcct atcggcaaag cactcaaaac
      601 ccgccacatc ttcctgtccg accaaccgtt caaaactttc attttacccg accacgcaag
      661 ccgccgaaca aaaaat
//
LOCUS       DAXHSJ010000267          673 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000267 DAXHSJ010000000
VERSION     DAXHSJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.267"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(1..425)
                     /locus_tag="ACFM8U_002072"
                     /pseudo
     CDS             complement(1..425)
                     /locus_tag="ACFM8U_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            408..>673
                     /locus_tag="ACFM8U_002073"
     CDS             408..>673
                     /locus_tag="ACFM8U_002073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335239.1"
                     /translation="MKEGAHGKSPKCLGGNLGDFGEFCKGLNLQIGFKTIRPNSSASK
                     NGMPIYSNVSRREIFDLYEQYSGQRPDFRNIPNKGQLSSTTITSG"
BASE COUNT          178 a          124 c          186 g          185 t
ORIGIN      
        1 acggcggcgg atgctttctc tacttttagg cctttttcgg tcagttggca gttaatcagt
       61 tccagcaatt cggacagggt gtcgtcttgc gccagccaat tgcggtaacg gcataaagta
      121 ctgtaatcgg ggatgctcag ttcgtcaaag cggcaaaaca ggttgaagtc gatgcgggta
      181 atgaggctgt gttcgagttc gggatcggag agactgtgtc attgtccgag caggacggct
      241 ttgaacatgg acaacagcgg gtaggcggga cggccgcggt gatctctaag gtaacgggtt
      301 ctttgacgat tcaggtattg ttcgatcggc tgccaatcaa tcacctggtc caacttcaat
      361 agcgggaaac ggtcgatgtg tttagcaatc atggcttggg cggtttgttg aaagaaggtg
      421 ctcatggaaa atctcctaaa tgtcttggtg ggaatttagg ggattttggg gaattttgca
      481 aaggtctcaa cttacaaatt ggttttaaaa cgatcagacc aaattcatct gccagcaaaa
      541 atggaatgcc gatatattct aacgtaagtc gaagagaaat atttgatttg tatgaacaat
      601 attcagggca gaggcctgat tttaggaata ttccgaataa agggcaatta tcttctacga
      661 ctattacatc agg
//
LOCUS       DAXHSJ010000268          653 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000268 DAXHSJ010000000
VERSION     DAXHSJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.268"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(52..537)
                     /locus_tag="ACFM8U_002074"
     CDS             complement(52..537)
                     /locus_tag="ACFM8U_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC6335240.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRFRNAIEHKKD
                     E"
BASE COUNT          192 a          209 c          121 g          131 t
ORIGIN      
        1 ttcaaaccgg aaaaatgccg tctgaacctt cagacggcat tccattcaca tttattcgtc
       61 ttttttgtgt tcgatggcgt ttcggaaacg attgccgccg taaggctctt caaccgtata
      121 ttccccctcg ataatatcgt catcgcggga aaagccctct tttctgcccg attggttcat
      181 gttgaaaaaa ttttccgcac ctcctgcctg caacactgca cctcccttaa acggcagcag
      241 cagcaatacc gccaacaccg aggatacgaa tcccggactc atcagacaca ccgccgccac
      301 cgtataacgg ataggccaca acatctgata aacggatacc ctcccgccgc ttctcattgc
      361 cgcgcccgcc aataaaagac cggacagccc cgtatgcctg agcatcagca cgccggcggc
      421 aaaggttgcc gccattagaa acagcgtcca accgccgccc aaccaatcgg caacccacac
      481 aatcgacata atctccaaaa acagcagcac caaaaaaccg ataccgaaaa atctcattga
      541 ccgtcatcct tatatttaag taaacagcaa accgcccgaa caggactcca agcgagctgc
      601 ctgtaaatga ttacaaaacc atgtgcttca agccgaaaca atgtgaaatc acg
//
LOCUS       DAXHSJ010000269          637 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000269 DAXHSJ010000000
VERSION     DAXHSJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.269"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(1..103)
                     /locus_tag="ACFM8U_002075"
                     /pseudo
     CDS             complement(1..103)
                     /locus_tag="ACFM8U_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(140..631)
                     /locus_tag="ACFM8U_002076"
                     /pseudo
     CDS             complement(140..631)
                     /locus_tag="ACFM8U_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          169 a          163 c          141 g          164 t
ORIGIN      
        1 gggttttcga caaatatcgg ttacgtttgg tttgcacttc cgccagcgga cggttgcggc
       61 aggctttgcg cataatgccg tcctgcagcc gatgctcttt cagttttccg taggtcggat
      121 tcttgaatcc gacattactt taatcgtatt caatagaaaa gtccgcattg ccgccacccc
      181 aattatgcgg ataaataccc tgtttgacat aacggtgaaa cgtagaaaac caccaatcgg
      241 aaatttgtcc tacatagcca tgtttgaccg gattgaaatg cagataatca aaatggcagg
      301 caaaatcggc ctcatcgcgg atagtatgtt cccaaaagcg tttttgccaa agcctgagat
      361 tgccgccgat taaatatttg ctgtgccgct tgatctgccg ccagcgttcc gaataagcag
      421 aatcattgtc cggcagccgc catatggtat gcagatggtc gggcattcaa cacccatgcc
      481 aaaatttcaa acggataccg ttcgcgcacc gccattaccg cctgccgtaa agccaaacgc
      541 accgcatcat cggtcaaaat cttctgccgt ttattggcta caaccgtaaa aaagtaagtg
      601 ccgccattgc ggtaaaacga cggtatttca tagtatt
//
LOCUS       DAXHSJ010000270          585 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000270 DAXHSJ010000000
VERSION     DAXHSJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.270"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            100..387
                     /locus_tag="ACFM8U_002077"
     CDS             100..387
                     /locus_tag="ACFM8U_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335241.1"
                     /translation="MDDLIAAFLGYLSGKYREGKISRLGISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWNFITEMMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
BASE COUNT          199 a           70 c           99 g          217 t
ORIGIN      
        1 ccaaagaggc tgaagggtaa gagacgctta tatcatagat atagtggcaa aacaggtaat
       61 gaattaaaga cattacctgt ttttacagaa atttttaaaa tggatgattt gattgcagct
      121 tttttaggct atttatcagg taaatataga gaaggaaaaa taagcaggtt aggaatatcc
      181 ctaacggcag gaagcatttt ctttgcagta ttctttattc atgaactgat aatttttatt
      241 gttgatggaa ataaggaaaa tctttggaat tttattactg agatgatgaa agatttattt
      301 ttattttcat gttctgtttg tttattattc tacttcttat tatttattgc ggaaactatt
      361 gggaaaacaa tcaataaaat taaataagat atttaagccg ctaaaaacca tttaagtatt
      421 ttatcttcta tgcggcattt atcttggaat tgaaatactg caatcgtgag gctaaggctt
      481 ccgttgtaac gtttaccata tatttcatta ttttaatttc atcaaaaaac aaaattcaag
      541 attatacata ccgccaatag tttcttgatt gttttgtata gtgga
//
LOCUS       DAXHSJ010000271          581 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000271 DAXHSJ010000000
VERSION     DAXHSJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.271"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            172..>581
                     /locus_tag="ACFM8U_002078"
     CDS             172..>581
                     /locus_tag="ACFM8U_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6335242.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSINNSYTKYANRIGLTDGNRFL
                     KDSSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRN"
BASE COUNT          197 a          115 c          114 g          155 t
ORIGIN      
        1 ttccgcaaaa tcacgacaca ctcggacacc cgccccgctt atcgcaactt tgcgaacgcc
       61 cccggaaaca gcaaagacat caaataattg attttattag aatctatttg caaagccatt
      121 tgccgttaca caagaatggc acattaaaat aactgatgag gatttataac gatgaagaca
      181 gacattcaaa ccgaattaac ccaagccctg ctaccacacg aaaaagtatg ggcgaacgaa
      241 gaaaaaacca ttttagccaa aaacattctg ttggatttgg tggaaaaaac cgacccgact
      301 attatcggtt tgttattgaa taatgatgag ttaaaacgcc atttctttgt ggaagtgaat
      361 ggtgttttgg tgtttaaatt gcaggatttt cgttttttct tggataaaca cagcatcaat
      421 aattcctaca caaaatacgc caaccgcatt ggtttgacgg acggcaaccg ctttttgaaa
      481 gacagcagtg atattgtgtt ggattttccg tttaaagact gtgtgttaaa tggcggacaa
      541 agcaccgaag aaggcgaaga aatttatttt aaacgcaata a
//
LOCUS       DAXHSJ010000272          559 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000272 DAXHSJ010000000
VERSION     DAXHSJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.272"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..173
                     /locus_tag="ACFM8U_002079"
     CDS             <1..173
                     /locus_tag="ACFM8U_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6335243.1"
                     /translation="HINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPS
                     QQLKILWDLGLI"
BASE COUNT          127 a          121 c          150 g          161 t
ORIGIN      
        1 accacattaa cggcattgag aacttttgga atcaggcaaa acgtgtcttg cgaaaataca
       61 atggaatcga tcgcaaatct ttcccgctgt tcttgaaaga atgcgaattt cgatttaact
      121 tcggcacacc gtctcaacag cttaaaatcc tgtgggattt agggctaatc tagtacagcc
      181 ccttagattt aattcactat acctgcgtaa tggtatcggg tactcatcat caacctgcct
      241 tgcattcggt tgtgttgtcc ggcaactgtc cggttgtgtc gatgggttgc cctgccgctc
      301 cctgtgctgc gcaacggttg tgtgccttgg gtgggcgcgg gtggcggccg ggcggtttgt
      361 tgctatgata cgggagttcc gatatacaag ggtgggcttc agcccaccaa tcaacgaccg
      421 aaaaagaaaa aacgttgggc tgaagcccac tctacgcaat tacgcaactg ttatcgtggg
      481 gatgacggga ttttaggttt ttgtttttct gtttttgcgg gaaggatggg ttttaggttg
      541 cggtcattta tcggaaaag
//
LOCUS       DAXHSJ010000273          553 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000273 DAXHSJ010000000
VERSION     DAXHSJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.273"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..>553)
                     /locus_tag="ACFM8U_002080"
     CDS             complement(<1..>553)
                     /locus_tag="ACFM8U_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335244.1"
                     /translation="RGDTYGGWRYLANLGDRYADNAAAIVGKDANLNGLNLWMKKGVE
                     NLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGRLPNTSEIERSYYKAVTYHGVS
                     SSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQAVNNPNGSESDNRKQLISALDK
                     GFDGSFKEKPFTFLQSVIMDVTKL"
BASE COUNT          169 a          135 c           77 g          172 t
ORIGIN      
        1 taactttgtt acatccatta tcacagattg taaaaaagta aaaggcttct ctttaaaaga
       61 tccatcaaat cctttatcta aagcagatat taactgcttt ctattatccg attcgctacc
      121 gttcggatta tttactgctt gctcattgtg gatacgttcg gcttctatcc ccaaacctaa
      181 tgcccaataa ccatccgcca tatccggaag tgaacgatta ataactaaat caatagctgc
      241 actagaagaa acgccatgat aggtaacggc tttatagtaa cttctctcaa tttcactagt
      301 gttaggtaat ctaccattat tttcattaat tagatctaca tattggctga aatgttgcaa
      361 tgcaacccga tcaaatttct ctaaacgggt ctttttaccg accgtatcat cccataggtt
      421 ttccacccct tttttcatcc ataaattcaa accatttaag tttgcatcct taccgacaat
      481 tgcggcagca ttatccgcat aacggtcacc caaattagcc aaataacgcc aaccgccata
      541 ggtatcgcca cga
//
LOCUS       DAXHSJ010000274          551 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000274 DAXHSJ010000000
VERSION     DAXHSJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.274"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(105..197)
                     /locus_tag="ACFM8U_002081"
     tRNA            complement(105..197)
                     /locus_tag="ACFM8U_002081"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(161..163),aa:Ser,seq:gct)
     gene            complement(354..>551)
                     /locus_tag="ACFM8U_002082"
     CDS             complement(354..>551)
                     /locus_tag="ACFM8U_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC6335245.1"
                     /translation="LAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
BASE COUNT          153 a          133 c          112 g          153 t
ORIGIN      
        1 aattcagaat caagacaggg caataccata ctggttttgc accaacccgc tataaaattg
       61 cataaaaaaa tagacactgt aaaacacagc gtctattttt tatctggcgg agtaagaggg
      121 attcgaaccc tcgatgcagg ttgaccccac atgcttcctt agcagggaag tgccttcagc
      181 ctctcagcca ttactcctaa ggaagctgta actataatca acttttccga taccgtcaag
      241 gaaaaatgga aagcaaatat taatcttttg aaataaaata agatttaccc tgattatacc
      301 caatcaggca cttcttttct tcctgaatgg aaatattcct gactgccaca tcctcaagca
      361 aaatgctttt gtgcatcctg tttgatttcc tgaaaccacg cgtcttcagg aaaacgtgca
      421 agcgcgacat ccaatgttga cagcaggttt tgcgacaggg ctttggtcag gtattcctcc
      481 tcgttggctt ctgccgcttt gtcgggctgt tgtgccaaac cgtcggcacg ggctttgtgt
      541 ccgtgtgcca a
//
LOCUS       DAXHSJ010000275          532 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000275 DAXHSJ010000000
VERSION     DAXHSJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.275"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(114..386)
                     /locus_tag="ACFM8U_002083"
     CDS             complement(114..386)
                     /locus_tag="ACFM8U_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335246.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
BASE COUNT          175 a          105 c           73 g          179 t
ORIGIN      
        1 cgtatgttgc acacccaatt tcaataggta aatcagattg caaatccagc aatttgaata
       61 ttgtcattgt tccgtgcaaa agggattttt attgatgagt tgtgtactgg gtttcagctt
      121 ggctttttag ataatctttt cacaagaaca tacatcaata ctgtccaaaa aaaaagattt
      181 gataaatata ttgcaacata ttttattcca cctccatccg aaacagaaac caatatattt
      241 ttaattaata catagctgat tattacattt attattccta taatatataa ggatcttgcc
      301 aaaatttttt ttgattttat tttaggaata tctcttatcc atgctaaaat acagcccaat
      361 gtcgaaaaga aaataacagc aaacactacc ttacctcctc tcacttccaa ccaaaccgat
      421 agcaggtttg gttggaagtt ggtttattta ttatttaacg gcgaatgtca gtgttcttac
      481 ccgtagaacc tgcataacca tttatgccac cagcaacagt ccccactgct ac
//
LOCUS       DAXHSJ010000276          498 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000276 DAXHSJ010000000
VERSION     DAXHSJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.276"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(1..492)
                     /locus_tag="ACFM8U_002084"
     CDS             complement(1..492)
                     /locus_tag="ACFM8U_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6335247.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIRDEADFACHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
BASE COUNT          147 a          129 c          104 g          118 t
ORIGIN      
        1 tcaatcgtat ccaatagaaa agtccgcatt gccgccaccc caattatgcg gataaatacc
       61 ctgtttgaca taacggtgaa acgtagaaaa cccccaatcg gaaatttgtc ctacatagcc
      121 atgtttgacc ggattgaaat gcagataatc aaaatggcag gcaaaatcgg cctcatcgcg
      181 gatagtatat tcccaaaagc gtttttgcca aagcctgaga ttgccgccga ttaaatattg
      241 gctgtgccgc ttgatttgcc gccagcgttc cgaataagca gaatcattgt ccggcagccg
      301 ccatatggta tgcagatggt cgggcatcaa cacccatgcc aaaatttcaa acggataccg
      361 ttcgcgcacc gccattaccg cctgccgtaa agccaaacgc accgcatcat cggtcaaaat
      421 cttctgccgt ttattggtta caaccgtaaa aaagtaagtg ccgccattgc ggtaaaaacg
      481 acggtatttc atagtatt
//
LOCUS       DAXHSJ010000277          485 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000277 DAXHSJ010000000
VERSION     DAXHSJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.277"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT          164 a           94 c           68 g          159 t
ORIGIN      
        1 actaactctt tagttaaaaa taatttacta acttttacta cttaggtata aattccaata
       61 cttaactaaa ctgagggtaa atatatttat ccaagataga agagaaactt ttttctctgt
      121 ttccgcaaga atagttacgg gttgtgaaat atgctctata cccaagcagt agatattttc
      181 attgttactt accgtattgc ttcatagaat tggcactcta cgctttcctt taaaaatcgt
      241 caataacgat tatcattttc tttaaattta ttcaaaacaa tattcttata cacatctcaa
      301 gcccgctgaa ataaagcaaa gtggtggttc atagatggca ataccgattt caaagcagcc
      361 tgcactttga caacacacga gaaatacggg aagaacaggg catcagcata tatttcagcc
      421 cgaagccgtt tcattcgttt aaaaaactgc ttttttcaca atttaccttt acaaagccta
      481 tacgt
//
LOCUS       DAXHSJ010000278          485 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000278 DAXHSJ010000000
VERSION     DAXHSJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.278"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..270
                     /locus_tag="ACFM8U_002085"
     CDS             <1..270
                     /locus_tag="ACFM8U_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6335248.1"
                     /translation="VKTETKTIFTAPTGGAQQRGTIPERPSSKPPYHESHSISSVGLG
                     VIAGVGFDITPKLTLDTGYRYHNWGRLKNTRFKTHEVSLGMRYHF"
BASE COUNT          129 a          152 c          113 g           91 t
ORIGIN      
        1 gtgaaaacag aaaccaagac tattttcact gcaccaacgg gaggcgctca acagagaggc
       61 actatcccag agagaccgag tagcaaacct ccctatcacg aaagccacag catcagcagc
      121 gtgggtcttg gtgtcatcgc cggtgtcggt ttcgacatca cgcccaagct gaccttggac
      181 accggatacc gttaccacaa ctggggacgc ttgaaaaaca cccgcttcaa aacccacgaa
      241 gtctcattgg gcatgcgcta ccacttctga ttccccgaca ccgatgccgt ctgaaccttc
      301 agacggcatt tttgatgcac ctaccgttta cagacgcggg gcgggcgtgg aaatacccga
      361 accgtcattc ccgacaacac cgtaatctcg aaatttgtca ttcccgcaca ggcgggaatc
      421 cagccccccg acgcggcggg aatctatcgg aaatgactga aaccccgaga ttctagattc
      481 ccact
//
LOCUS       DAXHSJ010000279          463 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000279 DAXHSJ010000000
VERSION     DAXHSJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.279"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT          113 a          138 c          113 g           99 t
ORIGIN      
        1 ccccctgacg cggcgggaat ctatcggaaa tgactgaaac cccgcgttct agattcccac
       61 ttccgtggga atgacggttc agttgcgctc cgacaacacc gtaatctcaa aacccgtccg
      121 acaacaccgc aatcttgaaa ttcgtcattc ccgcgcaggc gggaatccag acctgtccgc
      181 acagaaactt atcggataaa aacagttgct caaaccccgc gttctagatt cccactttcg
      241 tgggaatgac ggttcagttg cgtaggactg ggttgtcgaa agggacggat tcgatggatt
      301 cgatgaaaac ggtagaaatg ctggattgat ggaaatggcg ggctgaagcc cactccacag
      361 ccccgcccta caatacgcct tgcgaatctg tcccgcaccc gcccttgtat gcctcaaatg
      421 ggttaaacta cgcccttccc gacttcccgg gtacaggtcc gat
//
LOCUS       DAXHSJ010000280          449 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000280 DAXHSJ010000000
VERSION     DAXHSJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.280"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT          114 a           99 c          129 g          107 t
ORIGIN      
        1 tgcaggtttc cgtgcggatg gattcgtcat tcccgcgcag gcgggaatcc agaccttaga
       61 acaacagcaa tattcaaagg ttagctgaag ctttagagat tctggattcc cactttcgcg
      121 ggaatgacgg gattttaggt tcgcgggaat gacgggattt gagattgcag catttatcgg
      181 aaaaaacaga aaccgctccg ccgtcattcc cgcgtaggcg ggaatctagg ccattggaca
      241 gcggcaatat tcaaagatta tctgaaagtc cgagattcta gattcccact ttcgtgggaa
      301 tgacgggatg taggttcgtg ggaatgacgc ggtgcaggtt tccgtgcgga tggattcgtc
      361 attcccgcgt aggcgggaat ccagaccttg ggataacagc aatattcaaa ggttataaaa
      421 gacccgtcat tcccgcgcag gcgggaatc
//
LOCUS       DAXHSJ010000281          434 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000281 DAXHSJ010000000
VERSION     DAXHSJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.281"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT          120 a           88 c          112 g          114 t
ORIGIN      
        1 tgttaatcca ctatattatt tttgacacat tttcatgatt ttaaatcacg tcattcccaa
       61 gtaggcggaa accggcaatg agaagccgca gagacttacc aggaaaaacg gcaagccctc
      121 cgccgtcatt cccgcgcagg cgggaatcta gaaatgaaaa accacggtgt tatcggcaat
      181 gactgaaacc ggagaaatga cgaaattgaa gcgtgcggaa atgtgtcgga aatcgctgaa
      241 gccggataaa ctagattccc gcttttgcgg gaatgacggg attttaggtt tttggtttgt
      301 tttcgcggca atgatgggat tttaggtttt tggtttgttt tcacggcaat gatgggattt
      361 taaattgcag gtatttaccg ggaaaaacgg caagccctcc gccgtcattc ccgcgcaggc
      421 gggaatctag aaat
//
LOCUS       DAXHSJ010000282          430 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000282 DAXHSJ010000000
VERSION     DAXHSJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.282"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT          176 a           34 c           79 g          141 t
ORIGIN      
        1 gtcctagatt cccactttcg tgggaatgac gggatgtggg tttttgtgcg gatttgaacc
       61 agtaagggtg gtgtgggatt ggtggtttgc ttaggatctt ttggattgta ttttgtatat
      121 acatttactt gttgataaaa gataaaataa aattagaaac taaaagtgag aaaaaattaa
      181 taataatagg gatgtataaa tgtaaagact ccgtttcata gctaaggtta tctgaatata
      241 tggaaaaaaa gtaaaagtcc ataaaactaa aatatataga taatgctaat gataatagaa
      301 ttatcacttt cctagagtaa ggataatatt tttttatcgt aaaaaaatat aaaataggat
      361 aaataattgc catatagata gattttgtaa caaaattaaa aataaataat aaaaataatg
      421 taataaagta
//
LOCUS       DAXHSJ010000283          429 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000283 DAXHSJ010000000
VERSION     DAXHSJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.283"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..>429)
                     /locus_tag="ACFM8U_002086"
     CDS             complement(<1..>429)
                     /locus_tag="ACFM8U_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6335249.1"
                     /translation="LIFVNPLYITDKVVALLKANGVPAALVDMGEIRGFDTNGKRTWN
                     VGIRNLDDEEGVLANITMKDKAFATSGGYGTVMDKAGRFTHLFDPRTGAGTPRYKSMS
                     VMADDAAVADALSTAFSIMDLPLIRSVAESRRLKVWLVMP"
BASE COUNT          104 a          139 c           93 g           93 t
ORIGIN      
        1 ccggcatgac cagccaaacc ttaagccgcc tgctttcggc aaccgaccgt atcaagggca
       61 aatccataat ggaaaacgcc gtcgacaagg catcggcaac cgccgcatca tccgccatca
      121 cgctcatact tttataacgc ggcgtacccg cgccggtccg cggatcgaac aaatgggtaa
      181 acctgcccgc cttgtccatc accgtcccat agccgcccga agtggcaaac gccttgtctt
      241 tcatcgtgat atttgccaac acgccttctt cgtcgtccag attgcggatg ccgacattcc
      301 aagtccgttt accgtttgta tcaaatcccc tgatttcgcc catatcgacc agcgcggcag
      361 gcacgccgtt tgctttaagc agcgcaacaa ccttgtccgt gatatatagt ggattaacaa
      421 aaatcagga
//
LOCUS       DAXHSJ010000284          408 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000284 DAXHSJ010000000
VERSION     DAXHSJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.284"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..210
                     /locus_tag="ACFM8U_002087"
     CDS             <1..210
                     /locus_tag="ACFM8U_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein GemA/Gp16 family protein"
                     /protein_id="HFC6335250.1"
                     /translation="AAMLDKVEALLTVGGKHWNYAHAMARRMFGKDKVEYLDDTQLHK
                     LVAALQIAENRKTEKAGGDDGVRKS"
     gene            191..>408
                     /locus_tag="ACFM8U_002088"
     CDS             191..>408
                     /locus_tag="ACFM8U_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HFC6335251.1"
                     /translation="MAFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGE"
BASE COUNT          110 a           73 c          138 g           87 t
ORIGIN      
        1 gcggcaatgt tggacaaagt cgaagccctg ctgaccgtcg gcggcaaaca ttggaactat
       61 gcacacgcaa tggcgcggcg gatgtttggt aaggataagg tcgaatattt agacgatacg
      121 cagctacata aactggttgc tgcgttgcag attgcggaaa acaggaaaac ggaaaaagcg
      181 ggtggggatg atggcgttcg aaaaagttga acatttattg ccggataccg tgttggacat
      241 tgtggatgtc atcggactgg cagcgacgga acagctggtc aaggcgattg gcggggcgcg
      301 gtttaaattt ggtaagggca aggtggacac cgagcgtttg gcaattttgg tcgaagccat
      361 cggcgaagtg aaaacacatg agctgttgca ggtatatggt ggcgagga
//
LOCUS       DAXHSJ010000285          403 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000285 DAXHSJ010000000
VERSION     DAXHSJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.285"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            108..323
                     /gene="rpmE"
                     /locus_tag="ACFM8U_002089"
     CDS             108..323
                     /gene="rpmE"
                     /locus_tag="ACFM8U_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC6335252.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT          124 a          105 c           74 g          100 t
ORIGIN      
        1 attttatcga atatcctctt gacgaaacaa cagaaaatag cgagaatgcc gagcttgtct
       61 gaagcatagg tacatcccct atgcccgctt ttttgaaagg aaatcaaatg aaacaaggta
      121 ttcacccgaa ctaccacgaa gtcaacgtta cctgctcttg cggcaacaaa ttcgcaacca
      181 aatccgcgat ggaaaaagaa aacttcaata tcgaggtttg ctccctgtgc cacccgttct
      241 ataccggcac ccaaaaaatc gttgatacca ccggccgcgt ggacaaattc aacaacaaat
      301 tcggcaacct gttcaaacgc tgattgccgc tttgcaagaa ttaagccctg ccatcatttc
      361 gatggcaggg ctttttaata tttggcacac tgacttctta tct
//
LOCUS       DAXHSJ010000286          384 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000286 DAXHSJ010000000
VERSION     DAXHSJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.286"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            123..302
                     /locus_tag="ACFM8U_002090"
     CDS             123..302
                     /locus_tag="ACFM8U_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335253.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYERRLGLG
                     AKLKNKNPDTDEKRQ"
BASE COUNT           99 a           72 c          102 g          111 t
ORIGIN      
        1 atttgtactg tcttcggctc gccgccttgt cctgatttaa atttaattca ctatatttgc
       61 ccgtgcgttt gtgataaatt cggcactgta tttgatatga ttttaacaaa tcgggagggg
      121 caatgttttg gtatgtgatt ggcttttgcg cctttgtcgt cgcgctgctg tcgctgtggg
      181 tcaatgccgg cgcgttcggt atgcaggaag acgatactcc acaatcggat tatgaaaggc
      241 gtttgggctt gggtgcgaag ctgaaaaaca aaaatccgga tacggacgaa aaacggcaat
      301 aaagacaaca aatgccgtct gaaaggtttt cagacggcat ttgctttgga gcggatatag
      361 tggattaaca aaaatcagga cacg
//
LOCUS       DAXHSJ010000287          366 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000287 DAXHSJ010000000
VERSION     DAXHSJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.287"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(153..>366)
                     /locus_tag="ACFM8U_002091"
     CDS             complement(153..>366)
                     /locus_tag="ACFM8U_002091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335254.1"
                     /translation="VKEYQPPVGIFLFVCFEKCLFPDFAIPIRFCAVRCVLARTCSKS
                     HVIPVFEALIRDADFQYIFSATTTKA"
BASE COUNT          123 a           85 c           60 g           98 t
ORIGIN      
        1 ggtcttttct aaaatatgca gatattcgaa tatcgaattt gccttatgat tacggttttc
       61 tgtcttatct accttaaaac gccgatattc cgtttgaacc aaattatatt tgccgaaacg
      121 ctcaaaaatc tcccttacct gcccgacaga catcaagcct tcgttgttgt agctgagaaa
      181 atatattgaa agtccgcatc cctaatcaaa gcctcaaata ccggaatcac atgcgatttt
      241 gagcaagtcc gcgctaaaac acatcgtaca gcgcaaaacc ggatgggaat ggcaaaatcc
      301 ggaaacaaac atttttcaaa gcatacaaac aaaaaaatac caaccggagg ttggtattcc
      361 ttaact
//
LOCUS       DAXHSJ010000288          365 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000288 DAXHSJ010000000
VERSION     DAXHSJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.288"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            174..>365
                     /locus_tag="ACFM8U_002092"
     CDS             174..>365
                     /locus_tag="ACFM8U_002092"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6335255.1"
                     /translation="MTMQVFLTIPGYDVEAEIEKFVWMDAVIWQMPSWWMHEPWTVKK
                     YIDEVLTAGHGKLYQSDGRH"
BASE COUNT          104 a           94 c           92 g           75 t
ORIGIN      
        1 cgcctgcgcg ggaatgacga atccatccgt acggaaacct gcaccacgtc attcctacga
       61 acctacattc cgtcattccc acgaaagtgg gaatccagga cgaaaaatct ccagaaaccg
      121 ttttacccga taagtttccg caccgacagg tctggattcc cgcctgcacg ggaatgacga
      181 tgcaggtatt tctgacgatt cccggctatg atgttgaggc agaaatcgaa aaattcgttt
      241 ggatggatgc tgtgatttgg cagatgccga gctggtggat gcacgagcct tggacagtga
      301 aaaaatacat agacgaagta ttaaccgctg gacacggcaa actctaccaa agcgacggca
      361 gacac
//
LOCUS       DAXHSJ010000289          364 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000289 DAXHSJ010000000
VERSION     DAXHSJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.289"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(<1..283)
                     /locus_tag="ACFM8U_002093"
     CDS             complement(<1..283)
                     /locus_tag="ACFM8U_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6335256.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYIPS"
BASE COUNT           97 a           91 c          108 g           68 t
ORIGIN      
        1 aagaaggaat gtatgtaccc gaaacggcag gtgcagacgg cggaacataa gcaggaggcg
       61 tataaaccgg cgcgctttgc accggcggca cataaggcgc atcgccggca ggagccgggc
      121 tgtacggcgt tgctccatag gggttgttgt aaactgccga agacggcgcg tcctgcatac
      181 ctgaattgcc tgcaatgacg ggggcaggct gctgggtagc acaaccgccc aacagtgcgg
      241 caaaggaggc gcaagccgcc aaaagtgtcg tttgtttcaa cataagataa ccttcatgtt
      301 ccgatatata gcctgaatgc ggtatatcat aataaaaatg cgcgttcttc tcaagcgcaa
      361 agcc
//
LOCUS       DAXHSJ010000290          355 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000290 DAXHSJ010000000
VERSION     DAXHSJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.290"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT           91 a           80 c           85 g           99 t
ORIGIN      
        1 aaccgttttt ctcgataagt ttccgtgccg acagacctgg attcccactt tcgtgggaat
       61 gacggtggaa aagttgccgt gatttcggat aaattttcgt aacgcataat ttccgtttta
      121 cccgataaat gcccgcaatc tcaaatcccg tcattcccca aaaacaaaaa atcaaaaaca
      181 gaaatatcgt cattcccgcg caggcgggaa tctaggtctg tcggtgcgga aacgtatcgg
      241 ataaaacggt ttcttcagat tttacgttct ggattcccac tttcgcggga atgacgggat
      301 gtaggttcgt aggaatgacg tggtgcaggt ttccgtgcgg atggattcgt cattc
//
LOCUS       DAXHSJ010000291          342 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000291 DAXHSJ010000000
VERSION     DAXHSJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.291"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT           76 a           75 c           95 g           96 t
ORIGIN      
        1 taccgccgaa aacggtatcc ggacggcttg atttgagttt tggtattttt gcccgacggg
       61 gtgaaaaata cagttgctac ggctcgatga atcgtcagaa atacccgaac cgtcattccc
      121 gcgcaggcgg gaatctagac cggtcggtgc ggaaacttat cgggaaaaaa ggtttctccg
      181 tccctgaatc ctggattccc actttcgtgg gaatgacaga atgtaggttc gtgggaatga
      241 cgtggtgcag gtttccgtgc ggatgaattc atcattcccg cgcaggcggg aatctagtct
      301 gttcggtttc agttattttc gataaatgct tgttgctttt ca
//
LOCUS       DAXHSJ010000292          341 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000292 DAXHSJ010000000
VERSION     DAXHSJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.292"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..>341
                     /locus_tag="ACFM8U_002094"
     CDS             <1..>341
                     /locus_tag="ACFM8U_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335257.1"
                     /translation="YLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEML
                     AYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNV
                     GFGHNKNVSLYG"
BASE COUNT          110 a           50 c           80 g          101 t
ORIGIN      
        1 tatttgaatc aaatcagttt caaaatggaa aatgatacgt tcactttgga ttttagtggt
       61 cttgttcaag catttaacca tgtcaaagaa actaatccgc aaaaagcttt tgtggatttg
      121 gccgagatgc ttgcatatgg cgaacttcgt tcttggtatg aaggccgaag actaatggcc
      181 gattatgtgg aggaggcaaa aaaagcaggt aaatttgaag attaccagaa agtgttgggt
      241 caggagaccg ttgcattatt agctaaaaca tcgggtacgc aagcagatga tatcctgcaa
      301 aatgtaggct ttggtcataa taaaaatgtt tctttatatg g
//
LOCUS       DAXHSJ010000293          333 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000293 DAXHSJ010000000
VERSION     DAXHSJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.293"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT           86 a          104 c           74 g           69 t
ORIGIN      
        1 ccgcacaggc gggaatccag acctgtccgc acagaaactt atcggataaa aacagttgcc
       61 caaaccccgc gttctagatt cccacttccg tgggaatgac ggttcagttg cgttccgaca
      121 acaccgtaat cttaaaaccc gtcatttccg acaacgccgc aatcttgaaa ttcgtcattc
      181 ccgcacaggc gggaatccag acccccgacg cggcgggaat ctatcggaaa tgactgaaac
      241 cccgagattc tagattccca cttccgtggg aatgacggtt cagttgcgtt ccgacaacac
      301 cgcaatcttg aaattcgtca ttcccgcgca ggc
//
LOCUS       DAXHSJ010000294          324 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000294 DAXHSJ010000000
VERSION     DAXHSJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.294"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(1..324)
                     /locus_tag="ACFM8U_002095"
                     /pseudo
     CDS             complement(1..324)
                     /locus_tag="ACFM8U_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710767.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          118 a           70 c           47 g           89 t
ORIGIN      
        1 aaatacagaa actcaagaaa ataaccttgt gtattgacca tctcaagcaa ttcagaaaaa
       61 tcaagaaatt ttctgaccgt aaacaaacgt ttccctaaaa aaaacgatgt cttcaaaaat
      121 atcgaccaaa taatcatctt cgccgttttc atttttatgg gttaaaacaa cacgggaaaa
      181 atctgttttc agatgcttgc ccgcttgatt gttcggatta ttgtccggaa cgacaaaacc
      241 gtcctcaaaa tcaaagtaga cgttgcgtcc ttctaccttt atctctgtgc aataacaatc
      301 atgcagagaa ataccatccg aaaa
//
LOCUS       DAXHSJ010000295          319 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000295 DAXHSJ010000000
VERSION     DAXHSJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.295"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..300
                     /locus_tag="ACFM8U_002096"
     CDS             <1..300
                     /locus_tag="ACFM8U_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6335258.1"
                     /translation="GFKAADVHFIRSGSDLVLSASEQDNVRISGFFYGENHRVDTFVF
                     DDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGNVDANTQSVQQPLLVTPSA"
BASE COUNT           87 a           62 c           70 g          100 t
ORIGIN      
        1 ggatttaaag cagcagatgt tcattttatc cgttccggaa gtgatttggt gcttagcgct
       61 tctgaacaag acaacgtacg tatttccgga ttcttctatg gtgaaaacca tcgtgtagat
      121 acatttgtct ttgatgatgc agctatcagt aatccagatt ttgccaagta tattaatgct
      181 ggcaataatt tggtacagtc tatgtctgtg ttcggttcta atactgctgc gacaggagga
      241 aatgtggatg ccaatacaca atccgtacag cagccgttat tggtaacgcc atctgcataa
      301 ggagcctaat cacattcat
//
LOCUS       DAXHSJ010000296          302 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000296 DAXHSJ010000000
VERSION     DAXHSJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.296"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..>302
                     /locus_tag="ACFM8U_002097"
     CDS             <1..>302
                     /locus_tag="ACFM8U_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335259.1"
                     /translation="AAALSGDLANMLKAYSAAETKEAQLALLDNLIHKWAETDSNWGK
                     KSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSDKAKAAIDAARDRIAVLDA"
BASE COUNT           89 a           75 c           68 g           70 t
ORIGIN      
        1 gctgccgcat tgtccggcga tttggccaat atgctgaaag cttattctgc cgccgaaact
       61 aaagaagcac agttggcatt gttagataat ttgattcaca aatgggcgga aaccgattcg
      121 aactggggca aaaaatcgcc aatgcgactt tcaaccgatt ggacgcaaac ggctaatgaa
      181 ggtattgcac tgacaccatc ccaagtagca caactaaaaa agaacgcttt agtttccctt
      241 tctgataaag ctaaagcagc tattgacgcc gcccgcgacc gcattgccgt gcttgatgcc
      301 ta
//
LOCUS       DAXHSJ010000297          296 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000297 DAXHSJ010000000
VERSION     DAXHSJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.297"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT           76 a           68 c           92 g           60 t
ORIGIN      
        1 cggaaaaaac agaaaccgct ccgccgtcat tcccgcgcag gcgggaatct aggacgtaaa
       61 atctcaagaa accgttgtac ccgataagtt tccgtgccga caagtccgga ttcccgcctg
      121 cgcgggaatg acggttcagt tgcgtaggac tggattgtga aaaggggcgg attcggtgaa
      181 aacggcggaa atgtgggatt gatggaatcg gtgggctgaa gccctcccta cagagctttc
      241 agacggtatt gtttgcgttt tcgggatggg ggcaaatgaa acgccgacaa accgat
//
LOCUS       DAXHSJ010000298          290 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000298 DAXHSJ010000000
VERSION     DAXHSJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.298"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT          102 a           58 c           60 g           70 t
ORIGIN      
        1 tttttgttaa tccactataa tttattgaag atggctgaaa ttcaaagtta cagaaattta
       61 taaacaaaaa acagcaaccc gccgccgtca ttcccgcgaa agcgggaatc cagtcccttg
      121 accttaagat aatctttaat tcgtcattcc cgcaaaagcg agaatccgga agcgaaaagt
      181 ggtgggaatg tattagaaat gactgaaact caaaaaaccg gattcccgcc tgcgcgggaa
      241 tgacgattga tacgtttcga tatagtggat taacaaaaat caggacaagg
//
LOCUS       DAXHSJ010000299          286 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000299 DAXHSJ010000000
VERSION     DAXHSJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.299"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT           77 a           76 c           66 g           67 t
ORIGIN      
        1 tatcccgtca ttcccacgaa agtgggaatc tagaacctct gaattttcag acaacctttg
       61 aatattgccg ccgtcccgcg ttctagattc ccgcctgcgc gggaatgacg attcatcagt
      121 ttcccgaaac gcccgaaaaa ccgaaaccga gcaaaccgga ttcccgcctg cgcgggaatg
      181 acgatttata agtttcccga aacgcccaaa aaaaccgaaa tccgacaggc gggatttttg
      241 ctttcgtggg gatgacggga ttttaagtta cggtcattta tcggaa
//
LOCUS       DAXHSJ010000300          276 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000300 DAXHSJ010000000
VERSION     DAXHSJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.300"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            1..276
                     /locus_tag="ACFM8U_002098"
                     /pseudo
     CDS             1..276
                     /locus_tag="ACFM8U_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT           76 a           94 c           53 g           53 t
ORIGIN      
        1 tgtccatgtt caaagccgtt ctgctcggac aatggcacag cctctccgat cccgaactcg
       61 aacacagcct catcacccgc atcgacttca acctgttttg acgttttgac gaactgagca
      121 tccccgatta cagcacctta tgccgctacc gcaaccggcg acgcaagacg acaccctgtc
      181 cgaattgccg gaactgatca accgccaact gaccgaaaaa aacctaaaaa tagagaaagc
      241 atccgccgcc gtcgttgacg ccaccattat tcagac
//
LOCUS       DAXHSJ010000301          271 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000301 DAXHSJ010000000
VERSION     DAXHSJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.301"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT           90 a           34 c           92 g           55 t
ORIGIN      
        1 cgcaggcggg aatctagact ttagaacaac agcaatattc aaagattatc tgaaagtttg
       61 agattctaga ttcccacttt cgtgggaatg acgggatgta ggttcgtggg aatgacgaaa
      121 agtggcggga atgacgaaaa gttgtgggaa tgacgaaaag tggcgggaat gacgaaaagt
      181 tgcgggaatg acgaaaagtt gcgggaatga cgaaaagttg cgggaatgac gaaaagttgc
      241 gggaatgacg gaaagttgcg ggaatgacgg t
//
LOCUS       DAXHSJ010000302          269 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000302 DAXHSJ010000000
VERSION     DAXHSJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.302"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..>269
                     /locus_tag="ACFM8U_002099"
     CDS             <1..>269
                     /locus_tag="ACFM8U_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6335260.1"
                     /translation="IPDYSTLCRYRNWLAQDDTLSELLKLINCQLTEKGLKIEKASAA
                     VVDATIIQTAGSKQRQAIEVDEEGQISGQTTPSKDSDARWIKKNG"
BASE COUNT           87 a           81 c           61 g           40 t
ORIGIN      
        1 catccccgat tacagtacct tatgccgcta ccgcaactgg ctggcgcaag acgacaccct
       61 gtccgaatta ctcaaactga ttaactgcca actgaccgaa aaaggcctaa aaatagagaa
      121 agcatccgcc gccgtcgttg atgccaccat tatccagacc gccggcagca aacagcgtca
      181 ggccatagaa gttgacgaag aaggacaaat cagcggccaa accacaccga gtaaggacag
      241 cgatgcccgt tggatcaaga aaaacggcc
//
LOCUS       DAXHSJ010000303          269 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000303 DAXHSJ010000000
VERSION     DAXHSJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.303"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..264
                     /locus_tag="ACFM8U_002100"
     CDS             <1..264
                     /locus_tag="ACFM8U_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFC6335261.1"
                     /translation="FYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQT
                     DFRNISDREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH"
BASE COUNT           89 a           67 c           51 g           62 t
ORIGIN      
        1 ttctaccaac acaccaaaat aaccaaagca ttgaaagcgg agacttattt ttgtcgccct
       61 taccattctt gggagaaagg gctgaatgag aacaccaacg gactcatccg gcaatacttc
      121 cccaaacaaa ccgatttccg taacatcagt gatcgggaga tacgcagggt tcaagatgag
      181 ttgaaccacc gaccaagaaa aacacttggc tacgaaacgc caagtgtttt attcttgaat
      241 ctgttccaac cactaataca ctagtgttg
//
LOCUS       DAXHSJ010000304          254 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000304 DAXHSJ010000000
VERSION     DAXHSJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.304"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..>254
                     /locus_tag="ACFM8U_002101"
     CDS             <1..>254
                     /locus_tag="ACFM8U_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6335262.1"
                     /translation="RTDAEGYIEKLHITPANAHECNHLSPLLEGIADGTTVYADKGYD
                     SAENRQHLEEHRLLDGIMRKACRNRPLSEVQTKRNRYVVE"
BASE COUNT           70 a           74 c           67 g           43 t
ORIGIN      
        1 cgtaccgatg cagaaggcta tatcgagaaa ctgcacatta cccccgccaa tgcccatgag
       61 tgcaaccacc tgtcgccttt gctggaaggg atagccgacg gtacgaccgt ctatgccgac
      121 aaaggctatg acagtgcgga aaaccggcaa catctggaag agcatcggct gctggacggc
      181 attatgcgca aagcctgccg caaccgtccg ctgtcggaag tacaaaccaa acgcaaccgt
      241 tatgtggtcg aaca
//
LOCUS       DAXHSJ010000305          252 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000305 DAXHSJ010000000
VERSION     DAXHSJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.305"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            complement(65..>252)
                     /locus_tag="ACFM8U_002102"
     CDS             complement(65..>252)
                     /locus_tag="ACFM8U_002102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6335263.1"
                     /translation="NYKVSLPPVKISLDIPNILCYTFQFVRRFCSSEEPNYTPTGKNG
                     QCFQWDFFGEIRHVAVG"
BASE COUNT           76 a           45 c           73 g           58 t
ORIGIN      
        1 ggtcagagga gggggaggaa gggattgttg gaggtggtgc agcatttagc agaaataaaa
       61 aaccttatcc gacagcgaca tgacgaattt ccccaaaaaa atcccactga aagcattgac
      121 cgtttttccc tgtgggcgta tagttcggtt cttcgctgct gcagaagcgg cggacgaact
      181 gaaaagtata gcacagaatg ttggggatat cgagagatat cttgacaggc ggaagagata
      241 ctttataatt cg
//
LOCUS       DAXHSJ010000306          246 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000306 DAXHSJ010000000
VERSION     DAXHSJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.306"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT           86 a           66 c           45 g           49 t
ORIGIN      
        1 caaaaacaga aaaccaaaat cagaaaccta aaatcccgtc attcccacga aagtgggaat
       61 ctagaacgta aaatctgaag aaaccgtttt atccgataag tttccgtacc gaacggtcta
      121 gattcccgcc tgcgcgggaa tgacgattca taagtttccc gaaatcccaa cataaccgaa
      181 acctgacagt aaccgtagca actgaaccgt cattcccgca aaagcgggaa tccagtccgt
      241 tcagtt
//
LOCUS       DAXHSJ010000307          246 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000307 DAXHSJ010000000
VERSION     DAXHSJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.307"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..>246
                     /locus_tag="ACFM8U_002103"
     CDS             <1..>246
                     /locus_tag="ACFM8U_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011145223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFC6335264.1"
                     /translation="IYRYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVG
                     IENRPAIVDQKSRIGDWEADTIVGKGQKSALLTLVERV"
BASE COUNT           76 a           73 c           58 g           39 t
ORIGIN      
        1 catttaccgc taccttcgcc aagacaaaag caacggcagc acgttgtggc aacatctcag
       61 aatatgcagc aaaccctacc gcaaacgcta cggcagcaca tggaccagag gcaaagtacc
      121 caaccgtgtc ggcatagaaa accgacccgc tatcgtcgac cagaaatccc gtatcggcga
      181 ttgggaagcc gacaccattg tcggcaaagg acagaaaagc gcattattga ccttggtcga
      241 acgcgt
//
LOCUS       DAXHSJ010000308          244 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000308 DAXHSJ010000000
VERSION     DAXHSJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.308"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT           77 a           50 c           50 g           67 t
ORIGIN      
        1 atgcgttgag tttcagctat ttagaataaa ttttgaaact ctaatcgcgt cattcccacg
       61 aaagtgggaa tctagaaatt taatgttgcg gcactagcca aaaaaccgaa accgaacgga
      121 ctagattccc gcctgcgcgg gaatgacggc tgcagatgcc cgacggtctt tatagtggat
      181 tgagaccttt gcaataacat aggttactaa aattttatgc tcaatctcat tttcaaaatg
      241 caaa
//
LOCUS       DAXHSJ010000309          237 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000309 DAXHSJ010000000
VERSION     DAXHSJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.309"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT           61 a           62 c           65 g           49 t
ORIGIN      
        1 ctgtcgggca tctgcagcgg tttgctaaaa aacgctttac cgtgatcagt gtgcaaagtt
       61 aaaatgggga agtaagcttt tcaaccagca attcggcggg cgcggaatcg ggcggtttac
      121 cgaaccccgg cgttcgcggc gcgccgcccc gtcccgtgaa ggcaaactca aggaataaaa
      181 gatgaataaa acttggaaac ggcaggtttt ccgccatacc gcgctttata ccgccat
//
LOCUS       DAXHSJ010000310          228 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000310 DAXHSJ010000000
VERSION     DAXHSJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.310"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
BASE COUNT           53 a           54 c           60 g           61 t
ORIGIN      
        1 ggaacttatc ggaaacgacc gaaaccgaac ggactggatt cccgcttttg cgggaatgac
       61 ggcgacaggg ttgctgttat agtggattaa caaaaaccgg tacggcgttg tctcgcctta
      121 gctcaaagag aacgattctc taaggtgctg aagcacgagt gaatcggttc cgtaccatct
      181 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccact
//
LOCUS       DAXHSJ010000311          212 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M09 240264
            SAMEA1706878-rid23340143.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXHSJ010000311 DAXHSJ010000000
VERSION     DAXHSJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706878
            Sequence Read Archive: ERR484089
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378078.1
            Genome Coverage       :: 229x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 13:23:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,104
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,943
            CDSs (with protein)               :: 1,943
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 46 of 105
            Pseudo Genes (incomplete)         :: 63 of 105
            Pseudo Genes (internal stop)      :: 19 of 105
            Pseudo Genes (multiple problems)  :: 21 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706878-rid23340143.denovo.311"
                     /strain="M09 240264"
                     /db_xref="taxon:487"
     gene            <1..>212
                     /locus_tag="ACFM8U_002104"
     CDS             <1..>212
                     /locus_tag="ACFM8U_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6335265.1"
                     /translation="IAKQLNRHKSTISREIRRHRTQGQQYSAEKAQRQSRTIKQRKRQ
                     PYKLDSQLIQHIDTLIRRKLSPEQVC"
BASE COUNT           71 a           73 c           43 g           25 t
ORIGIN      
        1 aatcgccaaa cagcttaacc gccacaaaag caccatcagc cgcgaaatca gacggcaccg
       61 cacccaaggg cagcaataca gcgccgaaaa agcccagcgg caaagccgga ctatcaaaca
      121 gcgtaagcga caaccctata agctcgattc gcagctgatt cagcacatcg acacccttat
      181 ccgccgcaaa ctcagtcccg aacaagtatg cg
//
